LOCUS       DAZKOW010000001       115637 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000001 DAZKOW010000000
VERSION     DAZKOW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 115637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 115637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..115637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.001"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..1407)
                     /gene="eno"
                     /locus_tag="ACG912_000001"
     CDS             complement(109..1407)
                     /gene="eno"
                     /locus_tag="ACG912_000001"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFQ0511359.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1523..2278)
                     /gene="tpiA"
                     /locus_tag="ACG912_000002"
     CDS             complement(1523..2278)
                     /gene="tpiA"
                     /locus_tag="ACG912_000002"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFQ0511360.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2382..3572)
                     /gene="pgk"
                     /locus_tag="ACG912_000003"
     CDS             complement(2382..3572)
                     /gene="pgk"
                     /locus_tag="ACG912_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFQ0511361.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3682..4683)
                     /gene="gap"
                     /locus_tag="ACG912_000004"
     CDS             complement(3682..4683)
                     /gene="gap"
                     /locus_tag="ACG912_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0511362.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4724..5815)
                     /locus_tag="ACG912_000005"
     CDS             complement(4724..5815)
                     /locus_tag="ACG912_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFQ0511363.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5964..7298)
                     /gene="rpoN"
                     /locus_tag="ACG912_000006"
     CDS             complement(5964..7298)
                     /gene="rpoN"
                     /locus_tag="ACG912_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFQ0511364.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7358..7429
                     /locus_tag="ACG912_000007"
     tRNA            7358..7429
                     /locus_tag="ACG912_000007"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7391..7393,aa:Arg,seq:ccg)
     gene            complement(7621..8505)
                     /locus_tag="ACG912_000008"
     CDS             complement(7621..8505)
                     /locus_tag="ACG912_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0511365.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8592..9491)
                     /locus_tag="ACG912_000009"
     CDS             complement(8592..9491)
                     /locus_tag="ACG912_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFQ0511366.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9508..10014)
                     /locus_tag="ACG912_000010"
     CDS             complement(9508..10014)
                     /locus_tag="ACG912_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0511367.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10203..10823)
                     /gene="rpoE"
                     /locus_tag="ACG912_000011"
     CDS             complement(10203..10823)
                     /gene="rpoE"
                     /locus_tag="ACG912_000011"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFQ0511368.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(10899..11336)
                     /locus_tag="ACG912_000012"
     CDS             complement(10899..11336)
                     /locus_tag="ACG912_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFQ0511369.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11499..12329
                     /locus_tag="ACG912_000013"
     CDS             11499..12329
                     /locus_tag="ACG912_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFQ0511370.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12313..13680
                     /locus_tag="ACG912_000014"
     CDS             12313..13680
                     /locus_tag="ACG912_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0511371.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13699..14508
                     /gene="yidA"
                     /locus_tag="ACG912_000015"
     CDS             13699..14508
                     /gene="yidA"
                     /locus_tag="ACG912_000015"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFQ0511372.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(14551..14994)
                     /locus_tag="ACG912_000016"
     CDS             complement(14551..14994)
                     /locus_tag="ACG912_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0511373.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            15128..15499
                     /locus_tag="ACG912_000017"
     CDS             15128..15499
                     /locus_tag="ACG912_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0511374.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(15668..16450)
                     /locus_tag="ACG912_000018"
     CDS             complement(15668..16450)
                     /locus_tag="ACG912_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFQ0511375.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16447..17418)
                     /locus_tag="ACG912_000019"
     CDS             complement(16447..17418)
                     /locus_tag="ACG912_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFQ0511376.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            17510..18904
                     /locus_tag="ACG912_000020"
     CDS             17510..18904
                     /locus_tag="ACG912_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0511377.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            complement(19096..20451)
                     /locus_tag="ACG912_000021"
     CDS             complement(19096..20451)
                     /locus_tag="ACG912_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0511378.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(20481..21116)
                     /locus_tag="ACG912_000022"
     CDS             complement(20481..21116)
                     /locus_tag="ACG912_000022"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFQ0511379.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            complement(21123..22499)
                     /locus_tag="ACG912_000023"
     CDS             complement(21123..22499)
                     /locus_tag="ACG912_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFQ0511380.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            complement(22492..24273)
                     /locus_tag="ACG912_000024"
     CDS             complement(22492..24273)
                     /locus_tag="ACG912_000024"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFQ0511381.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            complement(24295..24606)
                     /locus_tag="ACG912_000025"
     CDS             complement(24295..24606)
                     /locus_tag="ACG912_000025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFQ0511382.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            complement(24596..25582)
                     /locus_tag="ACG912_000026"
     CDS             complement(24596..25582)
                     /locus_tag="ACG912_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFQ0511383.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            complement(25597..26181)
                     /locus_tag="ACG912_000027"
     CDS             complement(25597..26181)
                     /locus_tag="ACG912_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFQ0511384.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            complement(26202..26672)
                     /locus_tag="ACG912_000028"
     CDS             complement(26202..26672)
                     /locus_tag="ACG912_000028"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFQ0511385.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            complement(26691..28694)
                     /locus_tag="ACG912_000029"
     CDS             complement(26691..28694)
                     /locus_tag="ACG912_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFQ0511386.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            complement(28681..29013)
                     /locus_tag="ACG912_000030"
     CDS             complement(28681..29013)
                     /locus_tag="ACG912_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511387.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     misc_feature    complement(29113..29160)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            29350..30069
                     /locus_tag="ACG912_000031"
     CDS             29350..30069
                     /locus_tag="ACG912_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFQ0511388.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            complement(30399..32297)
                     /locus_tag="ACG912_000032"
     CDS             complement(30399..32297)
                     /locus_tag="ACG912_000032"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFQ0511389.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(32458..33720)
                     /locus_tag="ACG912_000033"
     CDS             complement(32458..33720)
                     /locus_tag="ACG912_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0511390.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(33713..34612)
                     /locus_tag="ACG912_000034"
     CDS             complement(33713..34612)
                     /locus_tag="ACG912_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0511391.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(34757..36535)
                     /gene="uvrC"
                     /locus_tag="ACG912_000035"
     CDS             complement(34757..36535)
                     /gene="uvrC"
                     /locus_tag="ACG912_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFQ0511392.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTCQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(36717..37031)
                     /gene="trxA"
                     /locus_tag="ACG912_000036"
     CDS             complement(36717..37031)
                     /gene="trxA"
                     /locus_tag="ACG912_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFQ0511393.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(37118..39478)
                     /locus_tag="ACG912_000037"
     CDS             complement(37118..39478)
                     /locus_tag="ACG912_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFQ0511394.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(39593..40141)
                     /locus_tag="ACG912_000038"
     CDS             complement(39593..40141)
                     /locus_tag="ACG912_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFQ0511395.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            complement(40199..40636)
                     /gene="zapA"
                     /locus_tag="ACG912_000039"
     CDS             complement(40199..40636)
                     /gene="zapA"
                     /locus_tag="ACG912_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFQ0511396.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            40838..41755
                     /gene="rnhC"
                     /locus_tag="ACG912_000040"
     CDS             40838..41755
                     /gene="rnhC"
                     /locus_tag="ACG912_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFQ0511397.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(42133..43044)
                     /locus_tag="ACG912_000041"
     CDS             complement(42133..43044)
                     /locus_tag="ACG912_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0511398.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(43445..44023)
                     /locus_tag="ACG912_000042"
     CDS             complement(43445..44023)
                     /locus_tag="ACG912_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0511399.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(44043..44711)
                     /locus_tag="ACG912_000043"
     CDS             complement(44043..44711)
                     /locus_tag="ACG912_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0511400.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(44726..45796)
                     /locus_tag="ACG912_000044"
     CDS             complement(44726..45796)
                     /locus_tag="ACG912_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0511401.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(45967..47331)
                     /locus_tag="ACG912_000045"
     CDS             complement(45967..47331)
                     /locus_tag="ACG912_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0511402.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(47328..48887)
                     /locus_tag="ACG912_000046"
     CDS             complement(47328..48887)
                     /locus_tag="ACG912_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0511403.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQISYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(48943..51423)
                     /locus_tag="ACG912_000047"
     CDS             complement(48943..51423)
                     /locus_tag="ACG912_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511404.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(51407..51742)
                     /locus_tag="ACG912_000048"
     CDS             complement(51407..51742)
                     /locus_tag="ACG912_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511405.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(51756..52790)
                     /locus_tag="ACG912_000049"
     CDS             complement(51756..52790)
                     /locus_tag="ACG912_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0511406.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(52880..53560)
                     /locus_tag="ACG912_000050"
     CDS             complement(52880..53560)
                     /locus_tag="ACG912_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0511407.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(53730..54689)
                     /locus_tag="ACG912_000051"
     CDS             complement(53730..54689)
                     /locus_tag="ACG912_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0511408.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            54852..55103
                     /locus_tag="ACG912_000052"
     CDS             54852..55103
                     /locus_tag="ACG912_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511409.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            55216..55458
                     /locus_tag="ACG912_000053"
                     /pseudo
     CDS             55216..55458
                     /locus_tag="ACG912_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            55548..55787
                     /locus_tag="ACG912_000054"
     CDS             55548..55787
                     /locus_tag="ACG912_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511410.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            55934..60217
                     /locus_tag="ACG912_000055"
     CDS             55934..60217
                     /locus_tag="ACG912_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFQ0511411.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            60316..60681
                     /locus_tag="ACG912_000056"
     CDS             60316..60681
                     /locus_tag="ACG912_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511412.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            60683..61135
                     /locus_tag="ACG912_000057"
     CDS             60683..61135
                     /locus_tag="ACG912_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511413.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            61584..63059
                     /locus_tag="ACG912_000058"
     CDS             61584..63059
                     /locus_tag="ACG912_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFQ0511414.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            63082..63393
                     /locus_tag="ACG912_000059"
     CDS             63082..63393
                     /locus_tag="ACG912_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511415.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            63754..65010
                     /locus_tag="ACG912_000060"
     CDS             63754..65010
                     /locus_tag="ACG912_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0511416.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            65007..65552
                     /locus_tag="ACG912_000061"
     CDS             65007..65552
                     /locus_tag="ACG912_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511417.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            65553..66053
                     /locus_tag="ACG912_000062"
     CDS             65553..66053
                     /locus_tag="ACG912_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511418.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            66412..66690
                     /locus_tag="ACG912_000063"
     CDS             66412..66690
                     /locus_tag="ACG912_000063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511419.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVMQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            66710..67054
                     /locus_tag="ACG912_000064"
     CDS             66710..67054
                     /locus_tag="ACG912_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511420.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            67073..67984
                     /locus_tag="ACG912_000065"
     CDS             67073..67984
                     /locus_tag="ACG912_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0511421.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            68063..69055
                     /locus_tag="ACG912_000066"
     CDS             68063..69055
                     /locus_tag="ACG912_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0511422.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            69052..69270
                     /locus_tag="ACG912_000067"
     CDS             69052..69270
                     /locus_tag="ACG912_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFQ0511423.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            69283..69693
                     /locus_tag="ACG912_000068"
     CDS             69283..69693
                     /locus_tag="ACG912_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFQ0511424.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            69745..72273
                     /locus_tag="ACG912_000069"
     CDS             69745..72273
                     /locus_tag="ACG912_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0511425.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            72289..74499
                     /locus_tag="ACG912_000070"
     CDS             72289..74499
                     /locus_tag="ACG912_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0511426.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            74507..75598
                     /locus_tag="ACG912_000071"
     CDS             74507..75598
                     /locus_tag="ACG912_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0511427.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            75619..76161
                     /locus_tag="ACG912_000072"
     CDS             75619..76161
                     /locus_tag="ACG912_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFQ0511428.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            76356..76613
                     /locus_tag="ACG912_000073"
     CDS             76356..76613
                     /locus_tag="ACG912_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511429.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            76627..76959
                     /locus_tag="ACG912_000074"
     CDS             76627..76959
                     /locus_tag="ACG912_000074"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511430.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            77115..78434
                     /locus_tag="ACG912_000075"
     CDS             77115..78434
                     /locus_tag="ACG912_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFQ0511431.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            78639..78926
                     /locus_tag="ACG912_000076"
                     /pseudo
     CDS             78639..78926
                     /locus_tag="ACG912_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            78985..79425
                     /locus_tag="ACG912_000077"
     CDS             78985..79425
                     /locus_tag="ACG912_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511432.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            79437..80105
                     /locus_tag="ACG912_000078"
     CDS             79437..80105
                     /locus_tag="ACG912_000078"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0511433.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            80169..80792
                     /locus_tag="ACG912_000079"
     CDS             80169..80792
                     /locus_tag="ACG912_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFQ0511434.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            80848..81234
                     /locus_tag="ACG912_000080"
     CDS             80848..81234
                     /locus_tag="ACG912_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFQ0511435.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            81447..82838
                     /locus_tag="ACG912_000081"
     CDS             81447..82838
                     /locus_tag="ACG912_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFQ0511436.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            83449..83817
                     /locus_tag="ACG912_000082"
     CDS             83449..83817
                     /locus_tag="ACG912_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFQ0511437.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            83997..84938
                     /locus_tag="ACG912_000083"
     CDS             83997..84938
                     /locus_tag="ACG912_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFQ0511438.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            85007..85738
                     /locus_tag="ACG912_000084"
     CDS             85007..85738
                     /locus_tag="ACG912_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0511439.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            85882..88062
                     /locus_tag="ACG912_000085"
     CDS             85882..88062
                     /locus_tag="ACG912_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFQ0511440.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            88082..88564
                     /locus_tag="ACG912_000086"
     CDS             88082..88564
                     /locus_tag="ACG912_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFQ0511441.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            88580..89341
                     /locus_tag="ACG912_000087"
     CDS             88580..89341
                     /locus_tag="ACG912_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0511442.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            89472..90806
                     /gene="murC"
                     /locus_tag="ACG912_000088"
     CDS             89472..90806
                     /gene="murC"
                     /locus_tag="ACG912_000088"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFQ0511443.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            91113..91598
                     /locus_tag="ACG912_000089"
     CDS             91113..91598
                     /locus_tag="ACG912_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFQ0511444.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            91591..92274
                     /locus_tag="ACG912_000090"
     CDS             91591..92274
                     /locus_tag="ACG912_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFQ0511445.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            92400..95045
                     /gene="polA"
                     /locus_tag="ACG912_000091"
     CDS             92400..95045
                     /gene="polA"
                     /locus_tag="ACG912_000091"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFQ0511446.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            95090..95926
                     /gene="mutM"
                     /locus_tag="ACG912_000092"
     CDS             95090..95926
                     /gene="mutM"
                     /locus_tag="ACG912_000092"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFQ0511447.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            95890..96519
                     /gene="coaE"
                     /locus_tag="ACG912_000093"
     CDS             95890..96519
                     /gene="coaE"
                     /locus_tag="ACG912_000093"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFQ0511448.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            96571..96903
                     /locus_tag="ACG912_000094"
     CDS             96571..96903
                     /locus_tag="ACG912_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0511449.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            97215..97706
                     /gene="nrdR"
                     /locus_tag="ACG912_000095"
     CDS             97215..97706
                     /gene="nrdR"
                     /locus_tag="ACG912_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFQ0511450.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            97729..99102
                     /locus_tag="ACG912_000096"
     CDS             97729..99102
                     /locus_tag="ACG912_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0511451.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            99102..100028
                     /gene="dnaI"
                     /locus_tag="ACG912_000097"
     CDS             99102..100028
                     /gene="dnaI"
                     /locus_tag="ACG912_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFQ0511452.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            100157..100597
                     /locus_tag="ACG912_000098"
     CDS             100157..100597
                     /locus_tag="ACG912_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFQ0511453.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            100741..101442
                     /locus_tag="ACG912_000099"
     CDS             100741..101442
                     /locus_tag="ACG912_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFQ0511454.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            101439..102560
                     /locus_tag="ACG912_000100"
     CDS             101439..102560
                     /locus_tag="ACG912_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFQ0511455.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            102575..103807
                     /gene="pepT"
                     /locus_tag="ACG912_000101"
     CDS             102575..103807
                     /gene="pepT"
                     /locus_tag="ACG912_000101"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFQ0511456.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            103939..104847
                     /locus_tag="ACG912_000102"
     CDS             103939..104847
                     /locus_tag="ACG912_000102"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0511457.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            104882..105154
                     /locus_tag="ACG912_000103"
     CDS             104882..105154
                     /locus_tag="ACG912_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFQ0511458.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            105165..106211
                     /locus_tag="ACG912_000104"
     CDS             105165..106211
                     /locus_tag="ACG912_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFQ0511459.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            106462..109071
                     /gene="clpB"
                     /locus_tag="ACG912_000105"
     CDS             106462..109071
                     /gene="clpB"
                     /locus_tag="ACG912_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFQ0511460.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(109222..109908)
                     /locus_tag="ACG912_000106"
     CDS             complement(109222..109908)
                     /locus_tag="ACG912_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511461.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(109886..110380)
                     /locus_tag="ACG912_000107"
     CDS             complement(109886..110380)
                     /locus_tag="ACG912_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0511462.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(110400..111620)
                     /locus_tag="ACG912_000108"
     CDS             complement(110400..111620)
                     /locus_tag="ACG912_000108"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0511463.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            111767..113602
                     /gene="lepA"
                     /locus_tag="ACG912_000109"
     CDS             111767..113602
                     /gene="lepA"
                     /locus_tag="ACG912_000109"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFQ0511464.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            113798..114709
                     /locus_tag="ACG912_000110"
     CDS             113798..114709
                     /locus_tag="ACG912_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0511465.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
BASE COUNT        36079 a        21589 c        22576 g        35393 t
ORIGIN      
        1 actctctacc tgctgatatt tcagcaagta gagagttcga ttacttaact aagtgatcat
       61 actcgactat ctttcaatag tcgatcatgt atcttcatta gaaaagaatt atttgttttt
      121 taagttgtag aaagatttca aacctttgta ttctgcaact tcaccaagtt ggtcttcgat
      181 acgtagtaat tggttgtatt tagcaatacg gtcagtacgt gatagagaac cagttttgat
      241 ttggccagca tttgttgcaa cagcgatatc agagattgtt gaatcttctg tttcaccaga
      301 acggtgagat acaactgctg tgtagccagc ttctttagcc atttcgatag cttcgaaagt
      361 ttctgttaat gtaccgattt ggttcacttt gataaggatt gagttagcaa ttcctttttc
      421 gataccttca gataattttt gagtgtttgt tacgaacaag tcgtcaccaa ctaattgaac
      481 tttgtcgcct aatacgtcag ttaatttctt gaatccgtcc cagtcgtttt cgtctaatcc
      541 atcttcgatt gagatgattg gatatttaga aactaattct tcgtagaatt tgatcatttc
      601 gtcagttgtt ttttcgcctt cgcctgagtc agctagtacg taaacacctt tttctttgtc
      661 gtagaattct gaagaagcag catccatagc aagaacaacg tctttaccag gtacatagcc
      721 agctttttcg attgcttcga tgattacttc aaaaccttct tcgtttgaac caaggtttgg
      781 agcaaatcca ccttcgtcac caacagaagt tgctaatccg cgagctttca agattgaagc
      841 taatgcgtgg aatacttcag cacccatacg tagagcttct ttgaatgttg gagcgccaac
      901 aggcatgatc atgaattctt ggaagtcgat tgagttatca gcatgtgatc cgccgttgat
      961 gatgttcatc attggtgttg gcaatacttt tgtgttgaat ccgcctaggt agtggtataa
     1021 aggtacttct aggtagtcag cagctgcacg tgcaacagcg atagaaacac ctaaaatagc
     1081 gttcgcacct aatttacctt tgttaggagt tccgtctaaa gcgatcatag ctttgtcgat
     1141 agccatttgg tcacgaacat cgtagccaat gattgcttca gcaatgatgt tgtttacgtt
     1201 gtcgacagct ttagttacac ctttaccgcc ataacgagct ttgtctccgt cacgtaattc
     1261 aactgcttcg tattcaccag ttgaagcacc tgaaggaacc attccgcggc caaaagctcc
     1321 gctttctgtg tatacttcta cttcgattgt tgggttacca cgtgagtcta agatctcgcg
     1381 agcgtaaaca tcagtaataa ttgacatgtt ttgtctctcc tttgagtttg ttttttccta
     1441 agggatttcc ctcatatacg attttagtta attttgctca ttagtgcaaa catttcttgc
     1501 atttttcgca aattttcaac aattatttta cagcgtctaa caatgacaaa aatgaatctg
     1561 cttgcaagct tgcgccaccg actaaagcac cgtcaacatt ttcttttgcc atgtattcag
     1621 caatattttc tggttttaca gaaccgccgt attggatacg aactgcttct gatacttctt
     1681 ttccataaag tttttcaact gtttgacgta caactccaca aatttcgtca gcgatttgtg
     1741 cgtcagctga tttaccagta ccgattgccc agattggttc ataagcaata accatgtttg
     1801 acacttgttc agctgaaaga tcagctaaac cagctgtaat ttgtccttcg atccattcag
     1861 cagttttgcc tgcttcatag gtttctaaag attcgccgca gcataggatt ggtgtcatac
     1921 catttgcaaa gatagctttt gcttttttat tgatgtcttc gtctgtttca tggaaatatt
     1981 cacgacgttc tgaatgtccg atgatcacat aatgaacacc tagatcagca agagctgctg
     2041 gtgaagtttc accagtgaac gcacctgagt tttcccagta gcagttttgt gctgagattt
     2101 ttagttctgt accttcagct gatgtcatta attcttgtaa gaataatgca ggagatccga
     2161 tgactgaatc aactttgtca tttgaaggga ttttgttttt tactgcttca gcaaattctt
     2221 ttgcttctga tgcagttttg ttcattttcc agttacctgc gataataggt ttacgcattg
     2281 aggaacacac tccttaattt attgaaaaat ggctagggag tcatgaaaac tcccttcacc
     2341 ttttgatcga ttactcgtca gaattgagca aacttggtcg tttatttatc gttgattgct
     2401 gctagtcctg gtaattcttt accttcaagc aattccaagc ttgcgccacc acctgttgag
     2461 atgtgtgtga atttgtcagc aaatcctaat tgttctgctg ctgcagctga gtctccgcca
     2521 ccgatgattg ttgttgcgcc ttctaagtta gcgattgctt cacaaacacc gattgtacct
     2581 ttagcaaagt tactcatttc aaatacaccc ataggtccgt tccaaacaac tgtttttgca
     2641 ccttgtaatg tatcagcaaa taattttact gatgctgggc cgatgtctaa agccatcaaa
     2701 ccatcaggaa tgtctccttc gattacttgt gtttcaacat catttgaaaa ttctggtgca
     2761 cagacattgt cgattggcaa gacaagtttg tcgcctgctt tttcgattaa ttcttttgct
     2821 agttccactt tatcagcttc tactagagag ttaccgatct tgattccttt tgcttcgtag
     2881 aatgtgtaag tcattccgcc accgatcaag attttatctg ctttaggaat caagttttcg
     2941 attacgccga ttttatcaga aacttttgcg ccaccaagga ttgcaatcat tggtcgtttt
     3001 ggttcttcta ctgcttcacc gatgaacttg atttcttttt ccatcaagaa tccagcaact
     3061 gttgggatac cagttgaagc gatacctacg ttagaagcat gtgcacggtg agctgtaccg
     3121 aaagcatcgt taacaaatac atcgcctaat gaagcccagt atttacctag ttctgtatca
     3181 tttttgcttt cttttttacc gtcaatatct tcaaatcgtg tattttcgaa tacgacaacg
     3241 tcgccgtcat tcatgttgcg gattgcttct tctaattgtt ctccgcgagt ttcaggtacg
     3301 aatgttactt ctttacctaa taattcgcct agacgttttg caactggagc taaagatttt
     3361 ccttctttat cttcttctgt ttttacacgt ccaaggtgag agaaaaggat tgcttttccg
     3421 ccttgttcta gaacgtagtt gattgttggc aatgctgctt tgatacgagt gtcgtcagtg
     3481 atcacgccgt ctttcaaagg aacgttgaag tcaacacgga caagaacttt tttgtctttt
     3541 aaatcgatat ctttcactgt tttcttagcc attttatttc ctcctgaata aacttattat
     3601 caaaaaaagc ggaagaagcg cgattgcttc cccgctttta aatgtacatg tctataattt
     3661 acacttgtac gtttgtctat cttataagtt agcgaagtat tctaaagtac gtactaattg
     3721 tgcagtgtat gacatttcgt tgtcgtacca agcaacagtt ttaactaatt gtttgtcgcc
     3781 aactgtcatt actttagttt gtgtagcatc aaataatgaa ccgtaagtca taccaacgat
     3841 atctgaagaa acgatttcgt ctgtgttgta tccgtaagat tcgtttgaag cttctttcat
     3901 tgctgcattg atttcgtcaa cagttacttc tttttcaaga actgttacta attctgttaa
     3961 tgaacctgtt ggtacaggaa cacgttgagc agctccgtct aatttaccgt tcaattcagg
     4021 aattactaaa ccgatagctt tagcagcacc tgttgagtta ggaacgatgt ttgcagcagc
     4081 agcgcgtgca cggcggaagt cacctttagg atgaggtccg tctagagtca tttggtcacc
     4141 tgtgtaagcg tggatagttg tcattaatcc ttcaacaaca ccaaatttgt cgttcaatgt
     4201 tttagccata ggagctaaac agttagtagt acatgaagca cctgagataa ctgtttcttt
     4261 accagttaat gtttcgtggt ttgtgttata aacgattgtt ggaacatcgt ttccgccagg
     4321 agcagagata acaacgcgtt tagcaccagc tgttaaatgt ttttcagctg ctgatttaga
     4381 agtgaagaaa ccagtacatt ctagaacgat atctacgcct agttcgcccc atggtaattc
     4441 ttctgggttg cggttagcta atactttaac ttcttttccg tttacgttga atgaaccttc
     4501 atgaacttca actgttccgt tgaaacgtcc ttgagttgtg tcgtatttta acaagtgtgc
     4561 caacatttta gcatctgtca agtcgttgat tgctactact tcgattccat ctacatcttg
     4621 gatacgacgg aatgctagac gtccaatacg tccaaaacca ttaataccta ctttaactgt
     4681 catttaagat ttcctcctta tgaaaatcaa aaaaatattt attttaaagg gttacccctt
     4741 ttaaaatctc atttgcagca gcttcatcag tgatgagcca cgtttgtttt ggtgcatttt
     4801 tcatataagc tcgaattgct ttagcttttg attttccgcc cgcgattgcc atgatgatcg
     4861 ggactttttc caaatcttgc aattctatgc cgattctagg gaccttataa acgacttcac
     4921 ctttttcatt gaaaaagtag ccaaaagatt ccgcaacagc atttgcttgt ttcagcatga
     4981 ctaattcttt ttcagtcatc ttacgacgtg ctgccatgtg taatgcacgc ccaatactat
     5041 gaataacaca attcgcttca ctaatcaagt ttaagacttc ttggatagat ggctcattga
     5101 gtaaagaatt gtaagttgcc agacttaatt gttccggaac ataaagtgca cggtggttac
     5161 cgtttgcttt agcagccatt cttgcactga cagaatttgc ctgtacagtt atcgcttcac
     5221 caatcccgcc tcttgcaggg acaaacaggt tatgccgttt cttattttcc agattagaca
     5281 attgttcggc taccacagcc atagtagttc ctcccatcac agcgatgata ttctcgcctt
     5341 ctggaagtag tcggtctagg gcttcgctca gcgcttcgcc gaatgcatca gccactttgg
     5401 tctgctcttc gctgtttcct gaaacaacga tacagcgttg gttaccaaaa tattctgcca
     5461 actgttgttc ggtttgatgc gtacctaaaa gctgatccat gaagttttca aggctttgat
     5521 aaacttcttc gcccttagtt gtcagcgtca taccgctttt tgaggtgtcg atcaaattca
     5581 gatttttcaa aagatctgtt tctgttcgca atactcgttc tgtgatcccc atactttccg
     5641 ataaacttcg acgtccaatc ggctgcatcc aatagatatt tcgtaaaatt ttatagcgtt
     5701 cttgcaacac ttcgataata tctggtgcaa cagattcaat catttttatt tcatccagca
     5761 tagcgatctc cttagttgga cactttacgt ccaatgtaga cttaaaacga cccatatacg
     5821 ataaaaaaag aaattaacta ttcgaatttc acatcaataa agggaaatct caacctcacg
     5881 actgtaagtc taacagtttt gctctcgttt ttcaactaaa aagactagaa aaagtgaaat
     5941 actttttcta gtcttttgac tgattagtta tcataacgtt ttctttttga tgaactcggg
     6001 attcctaata aatcacgata ttttgctact gttcgtcttg agatcgctat ctcatctttt
     6061 ttcagcaatt caaccaattt ctggtcagac aacggcttgt ttttgtcttc ttgatcgact
     6121 aattcctgca gctttttctt cgctgtatct gctgaaaggt cttctgtctg ttctgtacta
     6181 ttcgtaggaa ttcgtgtagt gaaaaatttt tttaattcaa atacaccgaa gtctgtttcc
     6241 aaatatttcc cgttgactgc acgactgacc gtggattcat gcacatctat ctcagttgca
     6301 acttccttta acgtaagagg cttgattgga cgttccttgt cgataaagaa agctttttgc
     6361 cgggaaacaa tgacttgagc aacccgcaaa attgtatccc cacgttgtag gatcgttttc
     6421 ttcaaccatt cgaattcctg tttccgttct tttaaataat tttcagtttc tttatctgct
     6481 tgttgtgaca tttgtttgaa gtaactctct tgaaagcgta tatttggctg attttttcgg
     6541 tttgagataa cttgtacatt tttatcgtcg tccactaata cccggacatc aggaataatg
     6601 tacaattcgc tgctacggtc gaaaatccgg ccagggcttg gactcaacgt ttgtaaatag
     6661 tcgaatatct gctgaacagc atgtagatca acagaaaaac gttgtgcgat ttctttccat
     6721 tttcgctcca ccagttcctc aaaacattct tccaacacga tataagctaa ttccggcgca
     6781 taatcatctc gttctgtttg aagcatgaga cattcttgca aactacgtgc acctactcca
     6841 gctggttcta attgctgaat cagagtcaac gcatcgagca tttggatagg cgtagctcca
     6901 atctcttttc cggcttcttc taaactaatc gtcaaaaatc cgttcaaatc gatgtattct
     6961 acaagataaa gaacgatttt cctcaaaaaa gtatctcggt aattcaaatg gacttgttcg
     7021 atcaatgaat caaataaaga cccttttgta tcaggaatct gatttaggta atttgtctct
     7081 tctccttcat aagaagatga agaagctttt atgtaatctg gctgccattc cggctcaaca
     7141 acttccacca atggattttc cattgcttga ttctcaacga atgccaacag ctcctcactg
     7201 ttatattgta aaacttggat ggactgttgc aactgttgcg tcatggctag tttctgtgtc
     7261 tgctgctgtt tttgtgaaaa attttgctca aatttcattt ttttgtccaa tccccttgta
     7321 attttttatc tgtttagtta caattatccc agtgcttgcg tccttagtgt agtggatatc
     7381 acgtaagatt ccggttcttg agacgggggt tcgattccct caggacgcgt tctactgcta
     7441 ttttagcata atttacgaat agttgcttct ttatttttaa gtgaaaactg tccgccaaag
     7501 aaaattggtg gacagttttt tgttgtacct gtatagatat tgttaagcct ttgcaagtaa
     7561 ggtagttatg tgttcaacta cttattaaaa tgtgttattt attaaagata cttgtctcac
     7621 ttactgaact ggccgatgat cgtttctgcc ataatagttc tcgacagccg ttggatgccc
     7681 taataaatcc ccaactgtcg tgatgcgata cccttgctct ttcagataat cgatcacttg
     7741 aggaacggca cgaatcgttt caggatagat atcatggaat aaaataattg ccccatcgta
     7801 tactgttgct tggatcttct gcaaaatcaa atcaggattt cttgtcttcc aatcttctga
     7861 atcaacagac cattcgatga tcggtcgctg gatgatcgtt gctcctacat tcgtgacaga
     7921 accatagggc ggacgaacaa aagtgggggt tttgccagtg acttttttga tctcagtgtc
     7981 tgctttcgtc acttcctccg tcatcgactg ggcagaaaga gtggccagat tctgatgatc
     8041 gtatgtgtga ttgccaatct cgtgccctgc ttcagctgtc tgcttcacga tttcaggatg
     8101 ctgcgccgta ttttctccta agacgaaaaa agtggcatgc acgttttttt cttttaagat
     8161 tcgcagaagc tgcggtgttg tggtcgctcc tggtccatca tcaaagctga ttgcgacgct
     8221 tttgacagcc ggatctcggc tcgtttttgt actgtcttgt gttgattgtg atgactgact
     8281 ttgtaccttt tccgctgatt cttgaatggc ttctttgacg ttagctgttt cagttgattg
     8341 gcttttaatg gttgattgtt ctgattgttt gaaagattgt gacgaatgct ccgaagagtg
     8401 tgtttgtcct tttacttcgg tgatcaagga ggccaccgaa gtaatttcga aaaacaaaag
     8461 cacggataaa gcaataatac tgataactaa ataatttctt ttcactatat ctcttctttc
     8521 tctttttctc ttcttattat ttacttgtac ttacttgaaa attgaaactg ctagtcgcaa
     8581 ctacttctcg tctaatgatc caaataatct tccgatgcca aaatcgtttt tgtcacggac
     8641 ttaggaatga caaattctgg tgtacccata taaccaggag caatttcgta ttgagaaaaa
     8701 gtcaaaacta attgatgctc tttgttcaaa taaaaacttc gtgtaggcgt aacaagtgtt
     8761 ggtttttcta tcgtcccttc ttgaatatct tgctcagtcc aatagtactt agatggatct
     8821 tccttgactt gttcggccat ctgccgtttg acttcttttg tcaaaacaga tagataggtg
     8881 tcatctttga ataaaagtgg aagtgagagc tggacactgt ttttcttgtc gattgtgtcg
     8941 tatctttttt caacatggct atctgctgct gtttgggtga tctgcctttc aatcacgaga
     9001 aaccggttat cgtctacgat tttctgataa tctcccaaaa cttggtaatg atccgtttca
     9061 aaatttccat attctttttc agctttttca aaatctgctt gtccttcgcg aaagtatttt
     9121 tgatttaatc catgaattgt tttattttct ttctcatttt gtgtattgat cttcggtacc
     9181 tgaatatcga ttttttcaga tcgatcagta aagcgttcgc ctaagatcaa ttcaatgact
     9241 gatcctaaga tgggtaaatc ttctgcaaag cttcgaactt gactattaaa aagaaggcta
     9301 cttgttgtaa tcattacgat agccaaagat actccgaacc cttggagacg tctctttctc
     9361 cgctttgctt ttataaatga ttgctcttcg ataataaaac gctggcggat ctcctcttta
     9421 agccctttgg gaattgcttg ttctttataa gttttcttta gtttttttaa attttctttt
     9481 tgggaataca tggcactctt ctctccttca ttctaggatt tgacgtaaag cattcaatga
     9541 gcggtacatt ttcgttttaa ctgtgttttc attcatatcc aagatagtcg caatttcttt
     9601 caaacgaaat ccttcaaaaa agtagaggat cagaatctct ttttccggac tgtctatttt
     9661 atctaaaatc atatggagct ctgcaacgga agtttctaaa ggtaactgtt gggggatatc
     9721 ttgaatttca aataagtcca caggcgtcct tttttgtctg cgccaactat ctaatgccgt
     9781 gcggatcaat atccgataaa accatgcact aaaatgctcg atctcttttt ctttttcaaa
     9841 atagttcagg gctttatgaa aactatcttg catcacatct aaggcctctt ctttatcatg
     9901 tacgtaattc atcgtgatac ggaacatttt ttgataatca gtttcgatga gtccattcag
     9961 caggtgacgg tatttttcga tctgccgttt tttcttatat ccagtaaaaa tcattctcca
    10021 ctcctttctg cctcacttaa tatgacgtac aaggtgcgct aaaagtttca atttttttat
    10081 tttttatgta tttacttggg atttttatat acaaaaaaga aggtgtgaca aaaatcgtgt
    10141 cacacctcct tattctgata ggctgatcgt ctctgtcaca acctctcgta agaaaataat
    10201 gtttattctt tttcagagtc agaaaattct tcctcttcat aatcttcatc atcatcgtct
    10261 tcgtctatga tacttaaatc ttcttcgata tttcctggca agtcttcttc atcatctgtc
    10321 gggtcatctg ctccgatttc ttgcaagtca gaattataag cagcgatttc ttcatcttca
    10381 tcatcatcgt catcaaacag atcatcgtca tcttcttctg tcagatctgt atcttctggg
    10441 tcatcgtcat tgtaatcgat cgcgtcttca tcatcattga taaaggcgtt gactttcttg
    10501 cgtttacggc gacgtggagt atcttcatcc tcttcgtcca ttccatggtt tacttcttca
    10561 tcgatcgaat cgatagcata ccatgaacgc aagccccaac gattatcccc taatgagata
    10621 aagctgccgt caatattcaa gtcagtataa aattgtgcga gtgaatcacg aatttcactg
    10681 tctgatttcc caagatagtt ttggatctgg ttagctaaat cagaaaaatc catgacatcg
    10741 ccacgttgtt ctaagatcgc gtgtgctact tcgatcattg ataattcgct tttgtttgca
    10801 ccttcaaata cactaatttc caatcaggac acgtcctttc acagtctact ccttatcata
    10861 caaaatcaga cacaaaaaat caaacttttc ttttatattt acgctgtaaa atccaaggtc
    10921 aaataatatt cgccgatttt ttccggtccc atgaacagat cataatcgat tcgaaccttc
    10981 cctgctgtgg gacggtcttt caaactaaca tgcagttctt tcgtaaaagt agcaatctgg
    11041 atcataccgt atggtgttcg ataggatgtt tctaaccgtt ctttatagcc aaatttcaaa
    11101 cgcatccgca tttctcctgc acggatcagc tgtacatgac catctggaac taatttgatc
    11161 gttacaggaa tctcatcgcc cgtctcctcc tgaatctctt tataacgtat gtacagcgta
    11221 tcgccaattg tcaccatttg ccctttcaaa tcaaaataaa aatcttgcac ttccccttgc
    11281 tgttttactt ttgtacgcaa tttgattgaa atcggtgttc cttgtgataa ttccattatt
    11341 cattctcctt tacttctcat agctacattg ttcctagttt cttttgtttt gtcaaccaac
    11401 ttgtccattt ttacagaaaa ccttttttcc aaaacaactc tttgatttta gagtataata
    11461 ttgtagaaaa tatgaattta tacaaaggag ccggttgaat gaaccctgcg attcgactag
    11521 atcagcacat ttatcaaaaa aacgactttt ccccttttgc attaacagat atattaacgg
    11581 aatattctaa agaacaccag caaatctttt tacatttctg gcaatatgac tgcacagtca
    11641 tcttaggtat gaaggatgtt cgaactcctt ttctaagtga cgggatccaa acactggaag
    11701 atgcaggtta cgctccagtg atcagaaatt caggtggatt agggatcgta tcagatgaag
    11761 gaattttgaa tatttctttg attttccccc aaagtgacac aaaaaaaata accatagatg
    11821 acgcttacga gcaaatgctc ctattgacta aatacgcttt tccaaaggca ccgatacaag
    11881 cattcgaaat cacccattct tattgtcctg gaacttatga tttaagcatc catggcaaaa
    11941 aatttgccgg tatcgctcag cgtcggatca aaaatgggat ttctgttatg atgtacttaa
    12001 gtgtcaacgg taatcaggaa aaacgtgggc aagtcatgaa gcgattttat caatctgctt
    12061 taaaagaaga ctttggtaca aatggctatc ctgcagtgga tcctgctaca atggcaacac
    12121 ttgaagaact gcttgaccaa cccctttttg taaaagaagt caaagaagca tttgcaaaag
    12181 cttttgaaaa gctgtattct gcaagtgatt tacaggattc taccgactgg ctaaaaaaag
    12241 aaatccagct ggagcaatgg aacaaccaaa tccagagaat ggaacaacgt aataacataa
    12301 aggagctaca ttatgacaac cccttataaa tatcaaatgc tgcctatgga aaaagttttt
    12361 cgtgatccag tccataacta tatccatgtc cagcatcaag taatcttaga tttgatcaat
    12421 tcgaaagaag tccaacgtct acgacgcatt aaacaattag gcacttcttc tttcactttt
    12481 cacggagcag aacatagccg ttttactcat tcattaggcg tttatgagat ttcaagacga
    12541 atctgcgata tcttttctcg aaatttttca aaagaaaaaa taggcaacgg aggctgggat
    12601 gatcaggaac ggctggtgac tttatgtgcg gcactgttgc atgatgtcgg tcatggtcct
    12661 tactctcata catttgaaca tatcttccat acgaatcatg aagccatcac tgtagagatc
    12721 atcacttctc cagaaacaga agtgtatcag attcttaacc gagtagaaga aggttttcct
    12781 gaaaaggtag ctagtgtgat ccaaaaaact taccccaacc cacaagttgt ccaaatgatt
    12841 tctagtcaga tcgatgcaga tcgaatggac tatttgcttc gggatgctta tttcactgga
    12901 acagaatatg gaacatttga tcttacccga atattacgtg tgattcgtcc ttatgaaggc
    12961 gggatcgctt tttccatgag tggcatgcat gcagtggaag attatatcgt tagccgatat
    13021 caaatgtatg tgcaagtgta cttccatcct gtttcccgtg gtatggaagt aattttggag
    13081 catctgcttc atcgcgcaca cgagttgtat caagaagatg ccagcagttt tgaactccat
    13141 tctcaattac ttctgccatt tcttgacgga agctttactt tagaagaata cctcaaactt
    13201 gatgatgggg tcttgggcac ttactttact caatggtctg aatctgctga tcctatttta
    13261 aatgatttag ctaaacgttt tttgaatcgt aaaccactga aatctgctac tttttctggc
    13321 aaccgggatt caaaattagt ccaagaactc actttgttag tagaaaaggt cggatataat
    13381 cctgtttatt acactgctgt gaattctagc tacgatctac cttatgattt ttatcgccca
    13441 gaacaaggca gacatcgcac acaaatcgag attttgcgaa atgacggaac tttgatagaa
    13501 ttatcacagg ttagtcagct tgttgctgca ttagcgggac aagaacaagg agatgagcga
    13561 tttttctttc caaaagaaat ggttgatccg tccttgcgtg atcattatga tttatttgac
    13621 gaaacttatc aagaatttgc cagccatatc cgtaatggcg cattgattga aataaattaa
    13681 aatatattgg aggaaattat gtctatcaaa ctagtcgcta tcgacatcga tggtacttta
    13741 ttaaatgaga aaagagaagt aacaaaagaa gtaaaagaag cgattgccgc tgctgttgca
    13801 aaaggtgttt cgatcgtttt atgcactggc cgtcccttac caggtgtcca ggaacagtta
    13861 aacgagttga acctttttca agataatgat tacgttatta cttataacgg ggcactcgta
    13921 caacaaacga aatcaggtaa aatcatcgcc cgtcacggac tgacacatga agatttccta
    13981 gagatcgaag taatggcacg ccgtgtcggc agtcatctcc attcgatcga tgacaaagcg
    14041 atctacacgc ctaatcgaga tatcagcgct tatacgatcc acgaagcaag tttagtcaag
    14101 atgccgctaa aatatcgaac acctgaagaa atgacaccag acatgaacat cgtcaaaatg
    14161 atgatgatcg acgaaccaga aattttagat gcagcaattg cgcgtttacc gcaaactttc
    14221 cgtgataaat acacaacggt caaaagtgcg ccctattact tcgaagtact aaataaagaa
    14281 gcaagtaaag gtgcggcagt tgcgaatctc gcacagcacc ttggtattga tcaagatgaa
    14341 atcatggcta tcggagataa cgagaacgac ttatccatga ttgaatatgc tggtctagga
    14401 gtagcaatgg gaaatgccgt ttctcttgtc aaagaagcag caaatgtcat cacttcatca
    14461 aacgacgagc acggtgtagc tgaagcaatc aaaaaatatg tactgtaata aataaaaaaa
    14521 ctgagagaaa gctgatctac tcgctttctt tcagttttta tttttttcga tccatctctc
    14581 gactgcccat cggtggctac cacgtttcaa tttcacaagc gcttcttgag ggctcaccca
    14641 ccaaatttta ttggtctttt cagttggttc tcctatacgc ttccaagaat cagcaacaaa
    14701 gaaatatcct ggattataat aataagttgc acgataattc gagtaaaaat actcatcagc
    14761 ttctcccaga taagaagaaa tttctgccac aattcccatt tcttctatca gctcacggac
    14821 aatcgcttct tctttcgtct cttctccttc aatttccccg ccaggaagaa aaaaagcacc
    14881 atttggagcc tcgattactg caatctgctg atctctttct atgacaatat atgctgcata
    14941 acgggtttga taatcagcaa attgcttttt ttcaccaaaa attggtttat ccatgaatag
    15001 tttccacctt aacagtttta ttttctatcc attttacatg agaaatttct catgaacaag
    15061 cacaaatcaa ctatttcctt acttcttttt gttatgattg agtaaaagga aaaggagaga
    15121 tgaatctatg aaaatggctc acacatgtgt tcgagtgaaa gatcttgaag catcaatcga
    15181 attttatcaa aaggcatttg gttttgaaga aagccgccgc cgtgattttc cagaaaacaa
    15241 atttacactt gtttatttga cacttcctgg ggatgactat gaattagagt tgacttacaa
    15301 ctatgatcat ggtgcgtatg atctaggaga cggctatggt catatcgcta tagcagctga
    15361 tgatctagaa aaattacacg agcaacataa agctgctggt cttgagatta ctgacttaaa
    15421 aggattacct ggcactgcgc cttcttacta ctttgtcatt gaccctgatg gttataaaat
    15481 cgaagtcatc cgaggatagt tagtatgggg cattttactc ttgattgaat aaaaaacaaa
    15541 caccaataat taaaatgaga cgcaaacttt tcattaaatt gaaaaagttt gcgtcttttt
    15601 ttaacaacca ttgtagctat aaggttgcca ctctccacca atttattttt aattcatttg
    15661 aaagaaatca aataaacgag gtacctttag aaaacaaaac tgcttttcct cctattatca
    15721 cacgatcttc ttttaattca cattctagat ccccgcccct tgatgacatt tgataagctg
    15781 acaattttgt tttattcatt ctttctgccc aataaggaat aagattagaa tgtgctgatc
    15841 cacatactgg atcttcattg attgttaatt ttggaaagaa ggttctagaa acaaagtctt
    15901 tttgcacact ttctgctgtc acgatgactc ctattcctaa ttcaaaattt ttgatagctg
    15961 taaaatctgg ctgcaattta gccaccgttt tttcatcttc taatacgaaa aataaatcac
    16021 gtgctaagta ggcttcttta atcttagccc caatcgcttc ttcatattca gacaatatcg
    16081 gcactttttt cggcataata ctaggaaaat ccatgtaata ataatcttta attcttgtca
    16141 caaatagatt accgctttga gtagaaaatt taatcgtttc atcaggtatt ttgtaatagt
    16201 taaataaaac aaatgctgtt gctaatgtcg cgtgaccaca aagatcgatt tctcgatctg
    16261 gcgtaaacca tctaagctca aattcttggt ttttcttcac tgtaaatgct gtttcagata
    16321 aattgttttc aatggcaatt ttttgcatcg taccttctgg cagccattct tctagcacat
    16381 aaaccgctgc tggatttcct ttgaacacct catctgcaaa tgcatcaact acgtaataat
    16441 ctaagttcat tttaaagccc ctttctctaa tttgttataa atatattctg cacaacaaag
    16501 atcttcaata gctagaccaa ctgcgtcgaa aatcgtcggc ctcgattctc ctttataaaa
    16561 tgtttttttc tcagaaacta attcgcctaa tgtaccaaca atacaagatt cctctaaatc
    16621 tccttctgcc atggctataa gcaggtcccc gctttctgtt aaggcagctg agtaatcatc
    16681 tacatagatt tgagaagttt ccatcaacga tgtacttatc tctcttgtat caggagtaaa
    16741 tgtgcccaca gcattgatat gtgcgtcttc ccttacccac tctttttgaa gaaatgcctc
    16801 tttactagag gtcaacgtac aaatgatttc cgctggacgt actgcatctt ctactgatat
    16861 tgcatttata aacgttattt taggatactg ttttttagct ttagcgataa aagtatttcg
    16921 tctttcttct aaaatatcgt aaacaaacac tgtagtgatg ggacgaatgg tagaaatcgc
    16981 agtcaaatgc gacaatgctt gttgaccagc tccaattaaa gcaagtgttt ctgcatgcgg
    17041 atttgctaag taatctgttg ccagcgcaga tacagcagct gttcgaatcc aggtgactgc
    17101 atttgcgttt atcaatgctt tgggtagtcc gttttgagca tcaaacaaga aaatctctcc
    17161 taaatgagaa ggtaaatgat actgatgatt atccggaaaa gcggtaatga cttttgtgcc
    17221 aaaatatctg ttctccccca aataagcagg catcatcgca aacatatttt tgccttttcc
    17281 gtcaggtaaa atttccacca atcgaggatt ttggctgatt ttaccacttt cataatcaaa
    17341 aaaacatttt ttcattactt gaatcgcttc ttcaaaacta aaagaagcta ctacttcctc
    17401 aaaattaata attttcatag acaattccct cctcacaaaa gattaacttc taaaatcaat
    17461 acaatcaatg gtaaaattgt atggtataca attttaagga gtggagaaaa tgcctattaa
    17521 ttcttatgac aattacccgt taacttggcg accagacaaa caaaaattga agcgtcctta
    17581 ttatcgatcg ttgattcaac aattagaaca agatatttta aagaataaat tacagaaaaa
    17641 cactcagtta ccctcccaaa gagagctagc tgattattta gatttaaatt ttactaccgt
    17701 tggcaaggct tataaatatg gaattgagaa aggtttgctg tatacgaata ttggaagtgg
    17761 cacatttatt tctcccaatg tctttgagtc catcaccatc tctaccaatg aagtaaccga
    17821 acatatcatt gattttggtc tggttagttc ttttgaagaa tgtaataaat tcgttgtccc
    17881 gtttattcaa tcaactagta ataacccaaa tcttgttggt ttgcttaatt accaagatcc
    17941 caaaggcaca aattttcaat tgactactgc agcaaattgg ttaaaaactc aaggagtcat
    18001 tccgtcctta gaatctctat ctattgtttc cggtgttcaa aatggattgg ctatcacact
    18061 ttctgcactt ttctctccag gtgatagaat agcaacagac cgatatacat acgctaattt
    18121 cattgaattg gctcagttat atcatttaga gatcgttcca attgattttg acgagcaagg
    18181 aatgcgacct gatatattga ttcaggaatg tagaaaaaag aagattcatg gtgtctttct
    18241 tatgccttct tgtaataatc ctatagggtt tcaaatctct cacaaaagac gagagcaatt
    18301 agccgaagtg attgaaaaag aaggtctatg ggttattgaa gatgatatcc attcattttt
    18361 aacaacttac tatcaacata cgatcattcc tcctttccaa aatttattac ctaaccaaac
    18421 aatctactta gctggaatga caaaatttat ttgttctgga ttgcgagttg cttatcttgt
    18481 agttccagta catgtccaaa gcaagataga acaagcaatt tttaatataa atgtgaaaac
    18541 ttctggatta gatgtggaag tcatcactca aatcctccat tcaaatgaag caaataggat
    18601 cttagaaact aaattaagtc ttacaaaaag agccaatcag ctttttgatt ctcttttcaa
    18661 agtagaaaaa ccaacgaatc gtgtccctta ctatcgaatt cttccagttg atcctgagat
    18721 tgaccctaag ataattgaga atgactttct tgctaacggt atccgacttt atcattccag
    18781 acgctttact acacgtgcgc aaccagatgc attcgttcga atttccctat cctctaataa
    18841 ccttgaaatt ttagcttctg gactaagtat cgtaaaagaa aaaataaaaa aatatgaatt
    18901 ataacctatt gataaataaa ggaatcaaat cttattgatt cctttcaata aaaaagaggc
    18961 actacccgag gttcaacaac gggtagtgcc tcaaaaaata aacgaacatt ttgcacttaa
    19021 agtatccaag aagcaagaaa aattatttta ttttcttatt ttactaaatc actaggtttc
    19081 tgtcagaaga tgaaattatc caatcatgac actttcttct ggatatttgt attttgattg
    19141 tgttctttgt gcttttgtca tcagagagaa gaccacagtc aatacaccaa ctcgtccaat
    19201 gtacataagc gctataatga tcaatttccc tattgcagtc aagtctggcg tcaatcccat
    19261 cgtaagacct actgtaccaa acgcagagaa gacctcaaaa gcaatatatt cgatgccaga
    19321 agttttaggt atcgtttcag tcaccgataa aatcatgatg gcgactacac acaacgtcaa
    19381 cgtaacaaaa aataaagtaa gtgcccgcaa cactgcgcct tgtcggatgg tacggccaaa
    19441 tgcttccgct ctcgtacgtc ctttcaacat cgaacgcatc tggatcagca acacacctaa
    19501 cgtagttgtt ttcaatccgc cggccgtcga cccagatgtt ccgccgatgt acatcaaaaa
    19561 catcgttaaa atcagacctg catggctcat atggaggtaa tcaactgaat aatatccagc
    19621 cgttctaggt gtcacactca taaaaaaggt gtttgccaat cgttgaaaca tattcccttc
    19681 cactagcgtt gccccattgc gctctgtgaa aaagaacaca acgaatccgc ctaataaaag
    19741 ggtacctgta acggttaatg cgatttttga atgaatcgtc atcttctttt tctgatgata
    19801 ttccagtaaa tcacgccata cgatgaagcc taacccacca gaaatgatca aagcagatac
    19861 caccatgatc aaataaacat tttgctggtg attggctaaa ctgtctccta acaaatcaaa
    19921 ccctgcatta caaaaactag agattgcatg gaacaaactg taccagatac ctttcaacca
    19981 gccaaattca ggcacaaata caaaagataa agcaaccgta ccaatcaatt ggatcacaaa
    20041 ggccaatttt aggatgtaaa gcatgagctt catcacacct gatacttctt ctagattcag
    20101 cgcttctttt aatataatac gtgtgctgag actgactttc ttctttatca atgcaaagca
    20161 tagaatcgga accatcataa aaccaagacc gccgatctca atcaaaagca tgatcaaaaa
    20221 ttgaccagca ctgttccaat gctctgctgt attcaatgtt gtcaatcccg ttacacagac
    20281 agcagaagtt gcagtgaaaa gcgcatcgat aaaatttgtc ccatgcccac tattactaaa
    20341 aaatggtagg gtaagtaggc cgccgccaac taaaatcagg agaaaaaatc ctaatgcgag
    20401 aagctgaaca gccggcaaac gtttctttat ttgtttgctt tgtttaatca tcgttttcct
    20461 cttttaaaaa aagataccac ttagtcagtg gtacccatat tttttacttt gatcaacctt
    20521 gttacttctg cgcgctcgtt ttcttccagc ttcatacgaa tataatagat cgtttcttct
    20581 aattgaggga tcgtcatata ttctaatgca ttgactcgcc gacgtgtctt ttcgatttca
    20641 ctcgccatca gttgacaagt tttttcgatt tctgtcagtt ccaaaagttt tggaagcagg
    20701 gtagtaaacc gatcgattga ccgatccagt tcggcactgg aattcaaata cccgtattcc
    20761 aaaggtgtgt ctgtcagctg ttcatcatat tcaaaattca tcacaggtac ttttacactc
    20821 atgatatttt tgtctacgat agacaaatca acgttttccg ccggcaaaac aaataattct
    20881 tcgatgaaag gttccgtgat cgaagcattt gccaatacaa aatcagacat tgcttctgtc
    20941 atagactctt ccatttcttg acgcagctgg ttgttctttc gaatcaacag gatgaattga
    21001 cgcatcagtt catcttgctt atcttttaat aatttatgcc ctcttgtggc agttgtcaac
    21061 tgttttttca atcgagtcaa ttccatgcgg gtcggattca cattcaatcg tgccatcaga
    21121 actcacttcc cttctggcag atatttgtcc agcatgtcgt ctttgattcg ttttaattcg
    21181 gttcttggca acatcgatag cagttcccac cctagatcaa gtgtttcagt aatcgaacgg
    21241 ttcgtgtaga atccttgatt gacatattct ttttcaaacc gctcagcaaa cttcgcatag
    21301 atcttgtcta catcagataa agcagattca cctaaaacta cggctaattc ttttgcctgt
    21361 tttccttgtg cataggctgc aaaaagctga ttcatcgtag ctgcatggtc ttctcttgtc
    21421 tttccgggtc cagttccctt atcttttaac cgcgaaagag acgggagtac atcaattggt
    21481 ggctgaatcc cactcttgta aagctctcta gtcaagatga tctgtccttc tgtaatataa
    21541 ccagtcaagt cagggattgg atgagtttta tcgtcttctg gcattgttag aatcggaatt
    21601 tgagtaaccg atccttttag tccgcggatt cttcctgctc gttcgaataa agtagctaag
    21661 ttcgtgtaaa gatacccagg atatcctctt cttcctggaa cttctcgccg tgctgcagaa
    21721 atctctcgca atgcctctgc atagttggtc atgtctgtca tgataaccaa aacatgcatt
    21781 cccttttcat aagcaagata ttctgcagca gtcaaagcca tacgaggagt agcgatacgt
    21841 tcgattgcag ggtcgtttgc taaattcata aacatgacag aacggtcgat tgcgcccgtt
    21901 tgacgaaaat cttccatgaa aaattcagct tcttcaaaag tgattccaat agcagcaaag
    21961 actacggcga aatcatctga tgaatcgagt accgttgctt gtcgcgcgat ctgagcagct
    22021 aattctttat gaggaagacc agagccagaa aatactggta atttttgccc acgaactaat
    22081 gtattcaaat gatcgattgc agagatacct gtttggataa actcatctgg ataatcccga
    22141 gctactggat tgattacttc tccgttaata tccaaatgct tttcaggcag gatctctggt
    22201 ccgttatctt tcggtcgtcc caaaccatcg aatactcgac cgatcatgtc ttccgataca
    22261 cctagttcta aaggatggcc taaaaatcgt acagaagagt cacgtaaatt gataccactt
    22321 gttccttcga atatttggac cattgcttta tcttcttgga cttccaatac ttgtccacga
    22381 cgaatctcgc cattttgcat acggacttcg attaattctt catatttgac accagcaact
    22441 ttttcaaccg ccatcaatgg accgacgact tcaccgatcg tacgatattc tttaatcatt
    22501 gctcatgcct ccttctgaaa cgatcgtttg catcgtttgc ttgatttcac caataatttg
    22561 atctagtttt tctaattctg tttccgggat atatttgctt cgtccgatcc tttcacgaat
    22621 cgatacggta ccgtccatga tctcacttaa ataagcacct aacgacaacg cacggcgtcc
    22681 ttcttcacca aaggtcaaga tcatcttcaa cattttgaat tgtttctctc ttgaagtgaa
    22741 tgtatcgaca tcatcaaaag cattttgctg caaataatct tcacgaatgg attttgccac
    22801 ttctaatgtc agacggtcat tatctgaaag ggaatcaatc cctaccaaac gcacgatttc
    22861 attcagctgc tgttcttcct gcaaaatacg catgccctcg gtgaccatgg atgcccagtc
    22921 agcttgaagg atatgatcca tatacttccc tacttctgtt gaataaagag aatagctttg
    22981 tatccaatcg atcgaaggaa aatgtctttt ttgggctaaa ctggaatcaa gaccccagaa
    23041 tacttttacg acccgcaatg tattttgtgt gactggttcc gaaatatccc ctccagacgg
    23101 ggatacagca ctgattgcgg ttatacttcc ttcgcgacct tctgatccta atgcaatgac
    23161 tcttccagat cgttcatagt attccgcaag ccgtgaacca agataagctg gataaccttc
    23221 atcaccaggc atttcttcca aacgtccgct catctcacga agtgcttctg cccatcgtga
    23281 agtggagtct gccatgatag caacgtcata acccatgtca cggaaatatt ccgcaattgt
    23341 gattcccgta tagatagaag cttctcgagc tgctactggc atgtttgatg tattagcaat
    23401 caataccgta cgttccatca acgattctcc tgtattaggg tcaatcaatt cagggaattc
    23461 atttaaaaca tccgtcatct catttccacg ctcgccacat cccacataga caaccatgtc
    23521 cacatcggcc cattttgcaa tttgatgctg aacgactgtt ttccctgcac caaaaggacc
    23581 aggtacagct gctgctcctc cttttgtcac tgggaagaat gtatcaatca ctcgctgtcc
    23641 agtgatcatg ggtgcatccg ggtttaattt ttctttgatt ggccgactac gtcttactgg
    23701 ccatttttga agcatcgtca aatttttgtc accattttcg gtttcaatga cacatactgt
    23761 ttcatcaata gtgaatgaac ctttttcgat tgttttgatc gttcctttta tcccatttgg
    23821 caccatgatt ttatgtttta taacttttgt ttcttctacg atcccgataa catctccagc
    23881 actcaccgtt tctccggctt ctactgttgg ttcgaaccac cattttttct catggtccaa
    23941 tgcgggcaat tgaactccgc gccctaagaa attactttgt gtgatttcca taaaggtatc
    24001 taatggccgt tggattccgt caaacatttg tgaaatgatc cctggtccta attcaacaga
    24061 taaagcttca ccagtagagc gaaccggttc gcctggacca attccagaag tctcttcata
    24121 tacttgaata gacgccacat cgccacgcat ctcgataatt tctccgataa ctcctagatc
    24181 tcccactaga cacatgtctt ggatactggc atcagacatg ttctcagcca ttacaagagg
    24241 tccggagact tttatgattt ttccaatttg caaaatttct atctcctttt catactataa
    24301 gatattttga cctactgctt tttctacgtt tttttgaatt tcttccagcc ctatccctaa
    24361 tgatccttga tgattgggaa tcaatacgat agcaggtatc atttgttcct tgtaacgttc
    24421 gatcgtttct gggctcaatg ctgcacattg ctctgtgaca taaatcacgc catactcatt
    24481 ctttgccatt tcttcaattg cctgacgaac ggtttgcttg gttgtaccat aacgaacatc
    24541 aaagccaaac aatttgaatg gcaaaacaga atctttgtcg ccaactacac cgatcttatg
    24601 cgtcatagac ttgtctcacc cgctttctga ttgtttctga ttcgaaatgg cttcttttgc
    24661 cgataaccag taaacgaagg tttttgcttt ctacctcttt tgcgttcaaa aaggctagta
    24721 gtggcagtgg tccgaaagcc tgggtttgtg cttcttgata caatgaactt agataatcat
    24781 ctttcaactg ttcaagcttt acgagatcaa tatttccctc acggatgaca gggcgcagaa
    24841 aatcagcata atctgtctct aatagaaaat tcgtgtaatc ttccatatct ttatctacaa
    24901 agttcaaaaa tacttctttt tcgatacttc ccgagctgga aagtaccgtc gtcatgaacc
    24961 catatgttct tttctgcaat attcctcgag ccatcgttgt gatattggtc aagtcaataa
    25021 aagcaatgac ttctgtcagc aattcttgat atcctagctt atcggcaagc cgtcgctgct
    25081 ctgtcaaaaa ctgtcgatca taaatcacgt caattccctg aagaatggta gactcttcaa
    25141 aataatcacg tacttctcgt atactgtcca taatgctttc aggaagttca tttgaaactt
    25201 gcgtatgaat ggcatccttc aaggtttcta atgaataaaa tccatcgtcg atatacaaat
    25261 ggtccagatt ttgatttgtc atctcagctt ttgtcaaaac cttcagatta tggaaagtat
    25321 aacgcatcgt atagatccaa acgatttcag gctccggtgc tgattccttt aaccagcgaa
    25381 atagacggct ctgctccttt aaaagattct gttcgaagtc atattcatat ccgtcataaa
    25441 tatatggact gtaaatcgtg gatttcaata tttcaccaag tgcttcgaca gaatttgttt
    25501 gaatcatccg ttcaaatgtc tcattggaca aaagttcaag ctctcttccg cgaatcaagg
    25561 gatttagttc gtggtattcc atacactgat ccctccttat tcgaataatt gattggcaat
    25621 ctcaaaactc atcgttcctt ggatatcttg tacaaggctg ctgaaaagaa aattatactg
    25681 aactccttga tcgtttatca gaaaacccgc ttgatcagca acagtttcat cacttaaaac
    25741 catcataaag ggaagttcat tgttccattc atccaacaac gtctgagaaa gataggctgc
    25801 tgatttttct ccagcaatga atgctacttt tcctgtcaaa ggtaatgaat acaatgcatt
    25861 tttgataaac tggatttgtt cagattcatc ccaattttcc atttcagtta ccgcatctgc
    25921 aaacaatcgt cttaaaaatt cctgcttcgc attcaaattt tcttgtcgta cttctacttg
    25981 ctggcgattc cttagctgac ggtatttttt ttcgagtaat tcgatttgtt tttctttttg
    26041 tttttgatgt tctgtttcaa cttgcgtacg tttttgttca aagttttgat cgatcttcat
    26101 tcgctcttct tgttctaacg ccgctctttc ctgctctgct gcttcgttca tctgagagat
    26161 gattttttca atggcgttca tctgcttttc ctcctttatg cttaggcatt caacactaat
    26221 aggaaggaaa taacgaaacc taaaatcgcg tatgtttcaa ccatcgcagc aaaaatgatt
    26281 ccttttgtag catgttcagg ttttttcgct aaaatttgaa tacctgccgc tgcgactttt
    26341 ccttgggcaa tacctgaaaa caagcctgta aaagcaatcg gcagagaagc acctagaaag
    26401 ttcagtcctt gtacaactga catgtcattt cctaagttga taaaaatcaa aaaggcaata
    26461 acgaatccat acaaaccttg cgtgcctgga agcaattgca gaatcagagc ctgcccgaat
    26521 ttttcgggtt gacttgttgt caatgctgct gctgcctcac ctgtcattcc aacaccttta
    26581 gctgaaccga tccctgaaaa aatcgtcgct gttgccatcg ctaataccgc aaataccatt
    26641 ccaccatttt gactaattaa gtaatccatc attatataaa aatcctccaa ttatttattg
    26701 ttttttttct tgtgattgat gttgacgtat ttttccgccg ttttcagcgg atcaaaggca
    26761 cgtccgcctc ctgtataaaa cttcccaaaa aattcgacat actgcaatcg tgcgccatgg
    26821 acataggcac ttagcagtgt caaaaacata ttcagtgcgt gtaaagcgat gatcaaaagg
    26881 atccctacac tgaatcgagc agctggcggc atgaaagcaa ctagcatatt gaatgccgct
    26941 ccaatacttc cgcctgaaat acccaatgcc atcaaacgtg tataactgac taaatcaccg
    27001 atatagccgg tcagtccgta aagattatac gcacctttgg cagcaccttt gactttagac
    27061 ggcgtttgga taatcggaac gatcaagata cataaggcag caatgatcga tagcccagcg
    27121 ccaaggtaaa caaatgctgt attttttatc aacatggcac caaccaacgc aataacgatc
    27181 ccaattagga tcccttgcca ggcaaatcca tcactgacag cactcaagta atcttttctc
    27241 ctgatatttt cctttgctgc cacaaataat ccaaccaaaa tctgtatcag accgaaaatc
    27301 acggataaaa tcaaaatcgt attgacgtca tccgtcgtag ataggattgg gaaaggcaag
    27361 cggatgccaa acagttcttt tggtaatgac tgtccgaaaa atgaactgta gatcaatccc
    27421 cagatgatcg atggtatcga gagaatctca aagaatttta cgaaacgttt catcccgcgt
    27481 ggtagaacaa gtagtttttg agctagtatt gtcccaatga gcatcagcaa accataacca
    27541 acatctgcga ccatcatccc gaagaagaca agataaaatg gtgtcatcca aggtgttgga
    27601 tctacttctt catattttgg cagactgtac atttcagtca gcaattcaaa aggttcgaca
    27661 agcggatggt ttttcaattt agtagggact tcctgctgaa tctcttctga gtttggtgtt
    27721 tcgaaactga cgtatatatc ttcttctgat acggtttcgt ttaacacatg cagcaaattc
    27781 tgtttttctt ctgcatctac ccaaccttgc aaaacgatca gataagtcgc atggataaac
    27841 cgttctttta ttttttctcg ttctgcaacg gcaagtgtga cttcttctac ccattcaaat
    27901 tcttggatat agccactgca ctgacctaac gctgtcgtta tcttcttttg ctgctcgtga
    27961 acaatggata attgctgctt ggtctcttgc agtttttctt taggattttg ttcataagga
    28021 tacgtttctt tattgaaccc gtatttccca gcaatttctt ctataacagc aactgatttt
    28081 tttaaagcaa catacgaaag ataaacttgt tttggcgtat aatctatttc ttccatataa
    28141 acgtcatcaa gcttactcag ttcttccttg aaactttcaa aatttgctgc actcacagtc
    28201 cccatctcaa aaatggtcgt cttacttgca tacatacttg gagcaatatc cagctgttgc
    28261 caatgaagaa gccattcttc ctcttcttct aaacgatcac gttcttcagt tagttgttcc
    28321 cacttttctt ttaaatcaag gacttctttc aatttattta tgaaattttc ttcggaataa
    28381 ttggcttcca aatcttgtaa ggaaagttct tgacgtttca aatggagttt cttttgacgg
    28441 ctatcgccgt ggtgttgtat gaattggata gcttcttgga tgtctttcaa ccgattacct
    28501 aaagcgctta attttgcttc tttatcaata atcgtggtat ctggaaaata cttctccacc
    28561 cactggttgt ttgttctatt ttgatacaga tcccggattt caacgttttg gattccttgt
    28621 actgcttgta aaatcctctc ctgatttctt ttatctgaaa tgagggtcac tttttccatc
    28681 ttagtgacgg ccataagttt ctttcaccct ttcaatgatc gcaccgatcg cttgactctg
    28741 atgcttttga tattgatctt tcaaagttgc ttgcaccttg tctgcttctg ctttcaattt
    28801 cattttttct ttttctaatt cttgtatttc aaattgttct ttttcttcca ccaactgttt
    28861 tagttgttgt cgatagtttg tttggatcgt ttccaattcc tgctcttttt ctgttgcata
    28921 gatcttcagc tgctgccgca gtgtttgagc acgcttttca ttttcttctt ctgcttttcg
    28981 gatttgttcc aaagcttcta acacttgctt cacttccttt cgatttaaaa aagaggtaca
    29041 tgctttcttt tttcattgaa agtatgtacc tctaaagata acttagtata ggcaggcccc
    29101 ttcaataaaa tgtccttctc accccttgaa gaactagatc cttgaagctt acgcccaacc
    29161 tttttatttc tgcttacttt ttattataac aaattaagca atattttcaa ctatcgtcat
    29221 gaatttcaca cgaaatttct ctttacagat agtaacacag caagaacaga taagtatagg
    29281 ggcaagtctt acatagttta atttttttta aatccttgtt acaataagtc tactttaggg
    29341 ggaatacaaa tgaaaccaat catcggcatt gccggaaatc aactgattcg agcaacagat
    29401 actttccaag gaaatcaagt gagctataca ccgcaaggat tcgtggatgc agttttagat
    29461 gctcaaggat tgcctcttat tctgccagtc atgtctccag attcagctcc tcaactgatt
    29521 ggacaaatcg acaaacttat tcttgcaggt ggtcaagatg tcagtccaca actttacatg
    29581 gaagatcctc atccaaagct aacggaaaca aatattcaac gagaccaatt tgaacaggca
    29641 ctgatcttgg aagcattgaa acaaagaaaa ccaatttttg cagtctgccg gggactccaa
    29701 ctattgaatg tcgttttgga aggtacactt tatcaagatc tttcattata tccaaagtgg
    29761 tcagtaaaac atgaacaaca gcccactgcc ccgcaattta cgactcacga agtcaagatt
    29821 gtttcagaca gtttgttaag caaccttctc cccgatccat attttgtgaa ttcttaccat
    29881 catcaggcag tcaaagatct ttcaccttta ttaaaagcca tcgctttttc taacgatgga
    29941 ctcgttgaag cggttcaaag caaagatgat atgcataaaa tcctcggtgt tcaatggcat
    30001 ccggaactta cccaccgtgt acatccatct gaacagcgct tattcgattt ctttgtgaat
    30061 gaattttaat agactttctg agggggttgt gaaaaagctg tttagtttcg agtattaaga
    30121 aaaattttgt gaaaatggct tttcacattt ttgcataaat ttggcttaat accgagaaac
    30181 tggcttttga acaccgttta taccaagagg tcgtgagcct gccgttcaac tctgagtcac
    30241 aaggagcaat ttcaaaaaac agcttttcat gtttgttgaa tagtgtgact tgtgaccgag
    30301 gagttggcag aagaacaccg tttatccgga aaaagagact gcgacacgac ttttgtctca
    30361 gtctccagtc tatggaacaa tgttcactgg aattcttctc aatcgtcctt tcgatagtcc
    30421 aacagccttt tgagctgggt aatctgctgc tgcagttcca taccttttgt tgtatcacga
    30481 ttgagtttgc gtggtagttg ctgttcgatt acttttttca catttgtttg gtcggatttt
    30541 tttgcaaatt gttgttcaat aggcaaaaac ggataatgtg tcacgatttg gaaaccataa
    30601 gaattgcata atagtgaata accggcaatt cctgtcgttt tttgataagc tttggataaa
    30661 cctccgtcaa tgacaaatac ttttccattt gctttgatcg gtgactcccc tttttttact
    30721 tttactggcg tgtgcccatt aatgattgct gacccttctt cttttgtgtc gaattctgct
    30781 aaaatttttt cactgaacca ctcttcatct cgcaagtgat aataaggatt gcttttctcg
    30841 acgtgcgtat gaggctctgt gagaaaataa cgttcaaaag tcgccatttt actcttgccg
    30901 aataaaggcg aaaatggccc actccacgca taccaaatca aatcggttgc taaatcttct
    30961 tttgcagtca gatcttttgc gctttcagtt atctgttttt caaaaaagcc cagcagttct
    31021 tttccgcgat aagattggtc taattcaaat gctaagagct gtcccttctc gtctacaggt
    31081 atacagccat gaaacagcac atgctgattg ttcgtgagat acattccccc tttttcgatc
    31141 aaaaacgtca tatgtttttg cattctcaaa gagtgctgga atgaatacat caaactatca
    31201 atcacttttt gttcctcttc caagaatttt tttggatcat ctggctggat cgtttgaaaa
    31261 caagttcctt tcaactcata gagctgacct tctaaatcaa tcgttgatgc ttcatagtcg
    31321 attttttcca gcaatttacg atcgtccatt ttaaattcag gtcgacgatc caataactga
    31381 tgttctgatt tgaattgaag gatagaaagt gcctgatgga ttttttctaa ttcttcttgc
    31441 tctctttcgg atagattttt actgttttta ggcgcaaata ctggattctt ctgataagtt
    31501 tgatgagcaa aagaaaacaa tggacgtaaa ttcaacccat attctttctc taattcaaat
    31561 aaataattat agcgtgctgc aatccgtaaa agtgtcagta aacatgcctc agagccgcaa
    31621 tacgcaccaa tccacaaaat atcatgattt ccccattgga tatctacaga agaaaaatcc
    31681 atgattcgat ccatcacttt ggctgcttga gttcctcggt caaaaatatc tccaacgata
    31741 tgcaaatgat cgatgacaga tttttggatc gtctgtgcaa gtttcgttaa aaatttttct
    31801 tcttccttca tcaaaacaag ttgttcaatg atttttttta catagtcttt ttttccattg
    31861 atacggtcat cgatatacaa gagttcctca atgatatagc tatattcttt cggcaatcct
    31921 tttcgaactt ttgaccgggt atatttgact gtcgtaaacc gtaaaagatc gatcaaatga
    31981 tggatcagct gcgatttatc ttgttctttg taccaatcag tcgttaatgc atattctgga
    32041 taagccgtaa aaatagtgaa ttggctgata tccgtttccg tcatttgttc agaaaaaagg
    32101 tctttgatct tttctttaag atttcctgaa ccgatacgca gaatatgatc aaaagcagga
    32161 aattcgccat gtatatcact caaaaataac tctgttcctt ttggaagctg caaaattgct
    32221 tcgagattga tgatttccgt gatgacatcc tccgttgagg ggaatttttc tgaaagtaaa
    32281 tctaaataac ggtccattaa aacgcctcca aaaattggta tactcaatat tataaagttg
    32341 ttatacacta ttttcaatga atatatatcc aatatagtaa aataaataaa ataggtctat
    32401 acaaaaagaa gctgggaaca atagatgtcc agcttccttc tgtattttag gattgtttta
    32461 tgcttttttt cgttcattcc tcataattga aatcgattgt ttcaatgaac tccataatcc
    32521 gccttcgcta taaacgagta cattcgattt gtacatgttt gcagacaaga tcatcaataa
    32581 gaatgttacg gctacaagta ccaacaagga aatcaatgct ccacccatac cgactgtctc
    32641 atttgctaag cgaatcggca taatatatga agataagaat ggaatatacg aagtgatccg
    32701 aataacgata ttgtttggat ctgaagctcc tagtatcaat ccaagcatgt atccgcctag
    32761 agatagataa gtcactggca aaattgcttt tgctgtatct tctgctttgt tgaccaaaga
    32821 gccacataaa gctgctaaaa cagaaaaaat caaaatgcca aagatcatga atagaagaga
    32881 ataccacaag aatggtccaa tcaagttatc caatgagata ccgtctaata tatttttgac
    32941 catttccata tctttgaatt gactgtatcc tataccaaaa ataataccgt ataatgccat
    33001 ttgcgttaat gcaacaagta aaacacctgt taattttccg taaaaatgct tttgtgcagt
    33061 cgtactggac aggatcactt ccatgatccg cgtgcctttt tcagaagcga tctcttgtgc
    33121 aatgatctgc gcatacgtca aaataataat gaacaagaca atcgttgcta cataacttac
    33181 gacgtattgg actgtcgagt tatcttctcc gactgtcatt tttccttttg aatcaaaatt
    33241 gactttttgt cgagtcaaac ttgcttgttg gctcaagcta gctacttctt ctggtgaaat
    33301 gcctaattga ctcgctcgaa gcatcgattg caagccagtc agttgttgtt gtatcagtag
    33361 ttgtgtactt tgtcctaatg aattttcgct ataaagcatc ccatttatct tatcattctc
    33421 agttgagact actaaatacg catcgatttt ttcttccgaa agctcttttt gtgctttttc
    33481 ttcagaatca actgctttga actcaaaatc atctgttttg acttggctca tttgctgact
    33541 gatttgctca ttatcagcca atacgccgat tttattgaca tcactatttt gttgagcaaa
    33601 gtttcctgct acataaatga tacccagcat gataaaaggg actaatatca tgatcaagaa
    33661 agaaatcgac ttaacatttt ttaaatagac atctttggta ataatccaaa acttacgcat
    33721 cgatttcacc tgctttcaat ttgaaaatct cttccaaagt tggcggttgc tggttaaaca
    33781 taggaatgta gccgaactga gtcgcacgag taaagatttc tttcccagct tcaggatctt
    33841 ctaaggtaat ctctaacgta ttgtcttcat gaaggatcac ttttttcacg ccttcgattt
    33901 cttctacttc ctgttggctt aaaccagatt ccaagaataa cttcgtacgg ccaaatgatt
    33961 cacgaatctc atgtactttt ccattcaaaa ccatttttcc gtttcgcaac atgatcaaat
    34021 gatcgcagat tttttctaca ttatccatat tgtggctaga aaaaatgaca catgaaccat
    34081 gttttttcaa ttccaatatt ccatctttta gcaattcagc atttaccgga tctaacccgc
    34141 tgaatggctc atccaaaatg atcaatttag gttcatggat taacgtggcg atcagctgga
    34201 ctttttgctg atttccttta gacaaagatt ttactttatc cgtctttttc ccttttacct
    34261 gaaatttttc catccaaaag tcgatttttg gttcaatctc ttttcttgat ttccctctaa
    34321 gttctgcaaa ataaagcagt tggtcttgaa tcgttacttt cggataaagt ccccgttcct
    34381 ccggaagata accgatgaca tcataatctt tctccccaag atgatggcca ttccataaca
    34441 cttctccttg gtcttgtgtc aaaaaatcca agatcaaacg aaaagtcgtt gtcttcccag
    34501 ccccgttttg gccaattagc cccatgattt ttccatcggg gatttggaaa gagacatgat
    34561 cgacagctgt gtagttccca aaagttttaa caagatcctt gacttctaac atttcttcac
    34621 tccttcttca agaaatcaca tatatcctta gacaccagac gtaagcgatt tattatttac
    34681 caacaaaacg taaacgctct ttttgttgta cataaaatga taggacactt tactttcttt
    34741 gtaaagtgcc ctttcctcat tcctgatgta atttatcaaa tacgttttgt gcgacatttt
    34801 tcggcagtcc gatttcttgt aattcttctg tcgtggctgc tgtgatattt ttcaacgatt
    34861 tgaactcttt aagcagcatt ttttttcgtt ttggtcctaa cccttcaatt tcatctaatc
    34921 gggaagcaaa actgttttta ctgcgtagct gccgatggaa agtgattgcg aatcgatgga
    34981 cttcatcttg gattcgctgg agtaagaaaa actctggtga atttcgctca agcggtacta
    35041 cagccaaatc tgtaccgaat agcaactcat ttgttttatg tttatcgttt tttaccatgc
    35101 cagcaatagg aatatcgatt cctaattgat tttctaagac atcttttgcc acatctactt
    35161 gtccttttcc tccatcgatc aagatcaaat caggtaaagg caacccttcg cgaatcactc
    35221 gcgaatatct gcgatagatg acttctctca ttgatgcata atcatccggt ccgttgactg
    35281 tttttatttt atatttccga tattcttttc ttgccggttt tccatcaatg aatacgacca
    35341 tcgcagccac aggattcgtc cccatgatat tagagttatc aaaagactcg atacgtattg
    35401 gggtcgggat attcatcgcg tttcctaaac gttcaactgc tccgatcgtc cgttcctgtt
    35461 ttctggcaat cagatcaaac cgttcattca aagcaactga ggcattctta ctcgccaatt
    35521 caactaattt tttcttttct cctcgttttg gttgcagcac tttcgtagct aacaaagctt
    35581 ctacactggg cttgtcgatc gtatcaggaa tcaagacttc tttaggaatg aaatgctcat
    35641 tttcctgata aaactgaccg aggtaagtca agaaatcttc ttctgcttcg ttatagaaag
    35701 gaaacattga aacatctcgt tcaattagct ttccttgtct tacgaaaaac acttggacac
    35761 acatccatcc tttatcaatc gcataaccaa atacatctcg atccagtaaa tcagtatttg
    35821 tcattttttg gcaagtcatg attgtctcaa tggcacggat ctggtctcgg tattctgcag
    35881 ctttttcaaa ctccatgttt tcagcagctt tttccatttt ctgatggatc tctgactcga
    35941 ttttttcgtg tcctccgttt aaaaagtttt tcacttcatc aacgattttc gtatagactt
    36001 tcggatctac gtcgaaataa taaggacaca ggcattgtcc caaatgatag taaagacaag
    36061 gctccttcgt ctgactaggt ttgcattttc tcagaggaaa taatcgatcg aggatcttct
    36121 ttgtctcatt cgccgcaccg acatcgggat atggtccaaa atacagtgct ttgtctttta
    36181 gtaccttacg agtaatcacc aaacggggat atttttcatt cgttatctta aggaaaggat
    36241 aggttttatc atcctttaac ataatgttgt atttaggatc atttttcttt atcagattga
    36301 tctctaagag caatgcttcg atattcgatt ctgtaacgat gtattcaaag tcttcgattt
    36361 cactaacaag ccgttccgtt ttcgtattat ggctaccagt aaaatatgag cggacacgat
    36421 tttttaatat tttggctttg ccgacataaa tgatcgttcc atttttatct ttcatcaagt
    36481 aacagccggg ctgatcagga agtaaagcta atttgttttt gattcgttcg ttcatctggt
    36541 actccccttc atattcaatt aaagttatta tagcatgtct gtcaaaaaaa caatccagcc
    36601 tgacaacgta attatactca aaaaatatta atttgaataa ccttaactca accgcaaaaa
    36661 aaccgaggac ggaactggat tcagtcccac cctcagctta tttttgctgt aagtttttat
    36721 aaatattgat cgatcatttg acgtagttgt tcttttgaat gaacaccaac agctttttct
    36781 actacttgac catctttttt caacattaat gttggaatgc tcatgatacc gaattgttga
    36841 ggagtttctg gattttcatc cacatccatt ttagcaattc tgaattcttc ttcatcatat
    36901 tcttgttcca actgatccaa aatcggtgct tgcatacgac atggaccaca ccaagttgcc
    36961 caaaaatcaa tcaatacaag tcctttatct gtttcttcat taaatgtctt atctgtaatt
    37021 acttgtgaca tatcaaagac ctccattttt aaaatctatg actagtatag catttctata
    37081 cttggatagc gaaccatctg cttaccttta gtaagcttta cttaaattta acaattgtcg
    37141 caccatttcc gccttggttt gccggtgcaa actcaaagct tttgacgctc cgatggtttt
    37201 taagataatc agtaattccc ttgcgaagtg cgcctgttcc ttttccgtgg acaatagtca
    37261 cttgaggata accagctaga atggcagaat ccaaatattg atctacctca cttagcgctt
    37321 cttcgtatcg ttttcctcga aggtccaatt gattcggcac gtgactacta gattcagatc
    37381 gtacagcatg gataactcgt tgtgtcggct ctttttgagg agcaacaggc gtcagctcgt
    37441 cttcggacac attcattttt aatataccga tttctacttg ccactgattc ccgtttttac
    37501 gtaaaagcgt ccctctttgg ccgtaagtcg taaccaacac ttcgtctccc ggtttcaacg
    37561 ttttttgttc tttcgccttc ttcaatactt tatttttctt caaagtttct tcttgatgta
    37621 gatttgctaa ttgggtcttt gcatcgatca actgatgctc tttcacattt ccttgcccaa
    37681 tctgctgttg cattttacga atatcagcaa tgattttttc tgctttttct tccgcctctg
    37741 acacgacttc atttgctttt ttctttgctt tttccatttc tttttcacgt tcttcaaaga
    37801 aatagctgta agcttctttc aaatcatcgt gaagttcttg tgcctcagaa acaaaatgac
    37861 gcatttccag atattcggtc tctgccattt tccggcgatt ctctaaatct gtgatcattt
    37921 cgtttaaatc ttggctctca tcattcatca actgcttcgc ttcatcaata acttctgtat
    37981 caagacctaa tcggctagag atttcaaacg cattgcttcg tccaggaacg ccaatcagca
    38041 aacgatacgt tggacttaat gtatccacat caaactccat gctggcatta atcgtatttg
    38101 cccgattata tccataaact ttcaattcag gataatgcgt tgttgccatc acataagcag
    38161 actttttacc aagatcatct aagattgcga ttgctagagc agcaccttct tgcggatctg
    38221 ttcctgcacc gagctcgtca aataatacca gacttttttc attgactttt gataaaatat
    38281 ctacagtgtt cgtcatatga gaagaaaagg tcgatagact ttgctcaatc gactgttcgt
    38341 ctcctatatc cgcaaatact tcctcaaaaa taccgatttg actttcttca cctgcaggaa
    38401 tcggtaatcc tgcttgcccc atcagttgta acagacctaa agtcttcaac gtgatcgttt
    38461 tcccacctgt gttaggtcct gtaatgacga tggcttgata gtcttttccg attgtgatgt
    38521 cattagaaac tactttttcc tgatcgatca gtggatgtct tgcttgtttc aagacgacat
    38581 gattgtcttc actgattccc ggcacgatgg cttttacttc tttaccaaat cgtgcttttg
    38641 cattcatcag atccatttta ccaatcacat aagtattgtg gatgatttct tgacgatggg
    38701 gaacaagttc agcagaaagt tcacttaaaa tacgttgaat ctcatttcgt tcagcgattt
    38761 ggtattgacg caaacgatta ttcaaatcca ccacttgttt tggttcaata aataaagtct
    38821 gacctgaagc actttgatcg tggactacac cgccaaatac accgcggtat tcttgtttga
    38881 ctgggataac gtagcggtca tttcgcatgg tgatgatcgc atcacttaaa tactttgcat
    38941 ttttcccacg gacgatccca tctaactgtt cacgaacagc ttgttcactt cgccgaatgt
    39001 tctgtcttat gctctttaac tcaggagaag cgtcatcagt cacgcgtccg tcttcatcga
    39061 ttgcttcctt taaacggcgg ctcaaaacag ggatcgtgac taactgatct gcccacatat
    39121 acaaacgtgc aaattcgatt tcactgtcag aaagatcatc aatgaaacga ttcaattcgc
    39181 ttgtcgtaga caatactctg gaaacttgtg caagctccac tccattcaag tctgctccga
    39241 tctcaatccg tttcatatgg ggacggatat tttcaatttt aggtataggg atcccgcctc
    39301 gcagacgttg cactttcaat ccatcctcag tttcttgaag ccagtttgtg atcgtttgtt
    39361 tctcactcac tggtaccaat tctgccagtt cttcttttcc ttgtgcagtc actacaaatt
    39421 gtctgaccat ctgtttgact ttttcaaatt ccaatgtttc aaggattcgg ttattcattt
    39481 tatcacctta tctttcacta tgaaaaaaga gctgtgccgg acatacgcta cgcagccttc
    39541 ttaaataaac tactctgtac aaaaaacgaa atattttcca gcactatttt ccttaattca
    39601 aaatattcgt tacccaaagc tcatacatct tgttggtaag aaatggcgtt ctctcaacga
    39661 taaaacgagc gaaactatgc tcgccgaatt gattttggac cacatctaat ggaacaaagg
    39721 caagtaactg caaaatcaaa aatatagtaa cgtaagccat gattagtgac aggatgcctc
    39781 ctgcaatcca gtctacctgt ctcaagatcg gcacatacgt caatccatgg acaaaaaccc
    39841 ccacaaatcg ggtaagcagc cagccaatta gcaaaataat cagaaaagca gttcctgcat
    39901 aaaaagcttc atctaggtga aaggcaaata cctgatcaaa aaatgctaac tttgatgttg
    39961 gggtgacagc tgggtatgga atgtataatt ccaaatgact tgctaatggt ttgtaaaaat
    40021 gctgggcagc aataaatgac aatacatatc caatcgcgaa tatgatttgc aaagcaaacc
    40081 cgcgacgtgc tccagaaaaa aacgcaatta gcaataaaaa taaaatcagc aaactcagca
    40141 tgctacgttc ctttctcttc taactttttt aaatttttct tattttgata aaaggatctt
    40201 aattagaaga tttttgttta atttgtcctt ttcgtttctc gtttaaaatc tgttgtgctt
    40261 ccacatgatt tttaatggta tgatctgctt gaccattttc tttcaatact tcccgagctt
    40321 cgttctcgat tgcttcaatg cgtttcacac gattttcaag ctctgtaaat ttgatcattt
    40381 ttttctttaa ttctgctgtt tcttcttcca gctccaatat tctttcttgt ttcttcaatt
    40441 gatccgaaag cgcattgaca gccatcaaga ttgctgcttg ttcattatcc atttgtggtg
    40501 ataattgttt caattctgct aactgttcat tgatcagctt cgttacgata tccatatgat
    40561 gcttggtttc ccgtccgata atagtatatg tttggttggc aatcaccgct ttgtagcgtg
    40621 ttttttcgtg cgccatacga atcatttcct cctatttgat gaaagtttta tttagtgtac
    40681 catgaagttg gttctttcac cgttctccac aaatattttt cacatactta actattatat
    40741 gataaacttt aaaaaaatgc tatgtctcgc aatctcttag aaaacgaaaa gtcgtgagtt
    40801 gcggtatagt tttatgtata aaaacgagga gaattttatg agcaacgtca ttatcaaagc
    40861 aacaaacgac caacttagtc aaatgaaagc atactatcgc tcttatttgt taaataaaca
    40921 gattccttat actagctttg ctgcaaaaaa agacgggacg accattaccg cttatacatc
    40981 cggaaaagtg atgtttcaag gtacgaatgc agcaatagaa gctgataaat ggggggaggc
    41041 acaggataaa caagccaagc cagcaaaaac agcctcttct ttgccaaaaa atatcagtca
    41101 attatccgtt ttaggtagtg atgaggtcgg aaatggcagc tatttcggtc cgctaacagt
    41161 atgtgctgct tatgtgaaac gtgatcagct tgcagaatta cgtaaacttg gtgttagaga
    41221 ttcaaaagag ttaaaagata gccaaatcat ccagcttgct gacatcttaa aaaaaaccat
    41281 cccctataaa ctgcttgtct tatctccaga aaaatacaat gagatccaac caaaatacaa
    41341 tgccgttcgg atgaaagtcg ctttgcacaa tcaagcgatc catttgctgc taaaaaaaat
    41401 cgagccagaa aaaccagaag cgattttgat cgatcagttt acgccggaag caaattttaa
    41461 aaaatatgcg cgtttagaaa aaaatcaatt acaagaaaaa ctgtacttcg ttactaaagg
    41521 agaacaatat catttagctg ttgctgctgc ttctatcatc agtcgtgcca gctttttgga
    41581 agagttagac aaagcatcag ctgaagctgg aatcaccctt ccgtcaggag ctggaacgaa
    41641 atctgaccaa gtagcggcaa aactattgga gaaaggcggc atgcctatgc ttgaaaaata
    41701 tgccaaactt cattttgcca atacagaaaa agctttgaag ctactaaaaa aataatggaa
    41761 ctgtgacaaa agtcatgtca cagtcccttg ttccgaataa acggtgttca atcatctgat
    41821 cctcggtatt agctcaacaa acggcaaaat atgaggaact atttttccgt ttgtttctct
    41881 aatactcaga acaaaacgtt gatttcacaa cctttttctt aaacggtgtt ctcatgccaa
    41941 ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt gaaattgctc
    42001 cttgtgactc agagttgaac ggcaggctca cgacctcttc cttaaacggt gtttaaaagc
    42061 ttgcatctgc gataataagc ccggccaaac gaaaaatatg aggagctatt tttgtttggc
    42121 tgggcttatt tattactgaa aataagtggt caatgcttgt gtcaaacttg cagcaatctg
    42181 ctgctgataa ctgtctgtta ctaattcctt taaatcacta tcattattca tataacccat
    42241 ctcgattaaa atagaaggtt gaagattttc tcgaagaact tgatagttcc cagtggagat
    42301 tcctcttgaa tccagaggca gatcagtgaa ggagtcagca attgtctgtg ctaatgtttc
    42361 ctcttgtcca tagtaataat atgtcgtgat tccagttgct tcattcgcat attccgtaga
    42421 gtctgcgtgt aaactgataa agacatctgc ctttttttct tcactgtagt cgcatatatc
    42481 tccaagctgt actaattcat ctgtagtatg ggtcaaataa accgttgctc cagcatcctc
    42541 tagcgccttt tttactttta tggctgtact taatgttata tctttttctt ctgtttgttc
    42601 atcattgctt aatgccccag tatcatttcc gccatgccca ggatctaata caatcactgc
    42661 atcagatagc tcttccggtt ttgtttgtat attctttgtg actttggttc catcttcgag
    42721 ggctaaatat ccagtatagc catcatttgt cttgatctga taagcatttt ttgtttcaga
    42781 aagaaactcc gccagctctc cgccgtttat agttgcaagt ggggtagtta gcgagtcatc
    42841 tgtatatagt gtacggtcat tttttccgat tttgattaat gtctcttgtt ctgtatcatc
    42901 aaatgattcc tttgtctttg taaccgtact gttcgtactg ctgaccgttt gtccaactat
    42961 ctttttttga ccaaaaacat ctatttgaaa acagataata atgataatga ttgcaaatac
    43021 aaaagctcca attatttttt tcattcttga ctctcacctc gtagatccag actaagggcc
    43081 aaaacttaaa atcagcttaa gtaagcaaaa aaggggctgt aaaagatata accgtagaaa
    43141 ataacattac tgctaaaatg atttttttca tttttggcat atcctgaaat ctccttttga
    43201 ctattcgtgg tgattcatct acccaataaa tagtttcctt cattctacaa acaattaatt
    43261 tcttcgttat taaattaaaa cacttcctgg aacaccgaca taataaataa aaatataccc
    43321 tgataatcat gagagaataa caaatttgta tttatgaaaa actacctata taaagaagca
    43381 agatataacg atcttcgtca tatcttgctt cttcacctaa ctattttctc cctatataac
    43441 agctttacgc ttctcctaat acaccattca aatataaatc gatcaattgc gccacattta
    43501 gcggttcttc cacaggtctt ttcgaccgag tgatgtaagg tgctaacact aagaaaaaat
    43561 gccttgctgc gatttctttt gaaatcccat ttcttagcac agattggttt gctgaaaaga
    43621 tttcttcaaa aggttcacga taatttttct tccatagaaa gaaaagatga tctcttgttt
    43681 cctttgaaag attgctttca agatcgtgaa gcatcagaaa caaatcgacc agatgtgtct
    43741 caatcaaaaa ctgcgccatt tttgtcaagg ttacttgaaa tcccgtttcg cttgtttgaa
    43801 cgatttctct caaagctttt ccaacatctt ttaagtggtt ttctaaaact gccgtatata
    43861 gtttttcttt atcaccgaaa taatgataga gattgggctg ggtgatccca actttttgtg
    43921 cgatttctct tgttgaagtg ccgagatatc ccttggacat aaacaagtca gtcgcttctt
    43981 tcaaaatttt ttgctgtgta tctttgttgt atcgatggac catttttcca cctgcttttt
    44041 ggttattctt tttctttcaa tacaccatct tccatcataa atattctatc gcatttgtca
    44101 atcagccgtg tatcgtgagt aaccatgatc gttgctttat ttttgtcttt tgattctttt
    44161 gccaagatat cgactacttc tcgtgccttt tctgaatcca aactagcagt tggttcatct
    44221 gctagaataa tggacggatc attgtacaat gcacgagcga ttgcgaccct ttgccgttct
    44281 ccaccagata gttcttctgg atacttgttg atcaacttat ccacgccaag ctgctcaaat
    44341 aattcgtttg cttctttttg acgatgttca tgtttaatct tatctactaa taataactgc
    44401 ttttttactg tcagaaaagg aaccagattg gaagcttgca aaataaagcc aatttcttta
    44461 aaacgtagtt taacgcgttc gctttctttt tgattactga attcttgatc attgataaat
    44521 acagagccac tgctaggcgt ctgcaatccg cctgccaaag tcaaaaaagt acttttacct
    44581 gaaccagaag gaccgatgat tgcaacaaat tcccctttat cgatactcat ggttgtttct
    44641 ttcaatgctt taactacttg gtcgccatct tggaaacttt tttctacttc tttaaattct
    44701 attgctttca tgttcttcct ccttcttatc caattgcctt caacggatcg atttttacga
    44761 ttgtccgaac agaaaatagt gcgcctaaca cagctactac taacatcaac aagctaatga
    44821 cgccaaaaaa cagccaattg ctttggaatg gaaccgcatc aggtagaaca agggatgttc
    44881 ccactgttcc taatagacca agaagaatac caacaaatga taaaatgaat gtttgtgcca
    44941 ctacagaacg cgcaataaat cctcctgtga tcccttgtgc tttcattaca ccaaagatat
    45001 taatcttttg catcgtcagt acataaatga aaatcccgat cacaatagcc gcaataacga
    45061 tcaaaaatcc gatcataaat ccaaaagtca atacttgcgc attatatcca ggtaattcat
    45121 tgatgaattt agaaattttg atctgttcta aatcttcagg taaattactg attttacctc
    45181 tgacaactat tgcgttaatc cgtgcatttt cacttgtatc ttctttttcg aagcggattt
    45241 cttggtaagc attgatggtt gtatagagaa ctggtgagac attgaatttg gcatgatccg
    45301 taaagccagt tagtttaaac gtcttgtcac taccagataa cttaactgta tctcctatgg
    45361 ctagaccata ttcttctttc aaactaatat cgcctactgc ctcatcatcg ttatcaaaag
    45421 cttttccttc aacaagattg ggcatgatga attgattttt atcgataccg aagaagctaa
    45481 cattgatttt tccttcttct gtactgtctt tactagtcac gactccaggt gtttgggcaa
    45541 gatacgccac tttcccacct tcgacttctt ctgctatctt cttagtaatc atggacatcc
    45601 ctagatttga atttgcatct ttcgaaagca caatcgaatc tgcctcccac ttgtcgactg
    45661 ctgttcgatt atcttgagct aagccataag ctaacccagt caaaaaaaat accaggtagg
    45721 cgatcaaaaa catcacccct gcaactaacg cataacgtaa ctttgaatgc ataatttcat
    45781 ttaatgctaa aaacatttaa ctcctcctcc tgaaataatc attttataca acaattttat
    45841 cacttgataa atttttggcg aatattatgc tcaaaaaatt cttccgctat ttcattatat
    45901 gaatttttta ttctcgattg attaatattt attatatccc cacacaataa aagaccgtga
    45961 tatttctcac agtcttttat ctactctatt gctatttgct ctcttagcta gtactttaaa
    46021 agctaactca ttgaggtagt gaaaacgaga ggtgtattca gacagaaatc caaaatcctc
    46081 aactgctagc tgtggaatcg tagttttgtg tttcacgata tccgcaagca ggttcaaatc
    46141 tgataataag aattcttggc ttatcagctg tccgccaatt atttctaatg tatccggatg
    46201 gtagatcagc tttgcattta ctgttttgtt ttcttcgtcg tattgtggaa atagatcaaa
    46261 ttctcctgaa cacgaaccgg tattttgttc caagaatggc gcttcatctt ctgtcagccc
    46321 tacagttcct aaaaagtagt ctataatgac tgctgctgtt atcttttgtc tgaggtcgta
    46381 agcggttggc tgaccactca aagttagcgc tactgctctt gccatatgta cagctcgagt
    46441 aactaaaggt aaaaagagat gccgtttatt tttgtcaaaa ctgactggga cgatatctcc
    46501 aatcgcatag atgtccggat cacttgtctg cagatattca ttgactatca cagtctcatc
    46561 ctcatacaaa gccacttttt cttttaacaa tgtcgtatta ggacgagttt gggcagaaaa
    46621 aatgaccata tctgccttta ttgtttgaga cagaagttgt acggatacta ctttctcttc
    46681 tgattttttc caatcgatca agaattcatt tggataaaaa tttatttgct tagtttgcag
    46741 ctgattttga aaactgttga tcatatcctt atcgaaatat cttgataaaa cattcggcat
    46801 ataatcaact agatgaataa tgtatccttt atttctcaaa gcatctgcta attccaatcc
    46861 aatgtaacca gcaccgaaga tcactacttc ttttgcttct tttaaagcgg caagtacttg
    46921 cttagattgc actagatttt tataggtgta gacattttca gcttcaattt gtttatacaa
    46981 ggtgacagga gaggaacctg tcgctacgat taatttatca taggttatgg tctcgttttt
    47041 tccttctttg gatgcgagga tgagctgatg tttgtctgga tcaagttcag ttactctcca
    47101 ttcggtgatc agttttgccc cactttcttg aagagtccgc atgttatggg caacttcagc
    47161 taaactacgt atctttcctt ttaaataaag gttgattcca tttgaaacat agcctagtcc
    47221 atctttatga tcttcatcga tcagtaaaac ttctgctttc ggttgaagtt ttcttagatt
    47281 tagggcagct gtgatgcctt catgggaagc accaatgatc acgattttca tgttttattc
    47341 atccttaaat tcaatttttc taaataacat tcttttaaaa attggttcaa ataggggaaa
    47401 tcataattat attcgttgct agtgaataca aaaactttag cggacttctg tgacgggtac
    47461 tctttcaatt ggctagtcaa aatgatatca tactgctttt ctttctcgta ttcgactaaa
    47521 tcatatttga tcgtattcag caattgttcc tggatgattt ctttcacgat atccgccatt
    47581 agcctttcat aaggaagatc ataagcaatt tgcagacgta attctaactg cttgatcagc
    47641 gtccgtaaaa tagatgcata gcgagcaaat aattcaaagc gggcattttc ctcgtctact
    47701 tcagtaaaat caacaatagc aaatgtttgt ctgaccaatc tttcacataa aaccaggaat
    47761 tgtttatctt ctacttcagt taaccgctgt tttaatcctt cttggccaaa aatattcatc
    47821 catcctctaa aataggcaaa atgagcgtga ttttgcatca ctgtcaacgc aatcttttgc
    47881 gaaatctctt ctggaaaaac ataaaaagag aaatattctt ctatcgattg tttaaacgtt
    47941 cgattcaatt gagctactac gtcgtctttg tatttttcgc ttatcaaaaa ctcctggaca
    48001 tgcgagttat gtgtatctaa tgtaaagtta ctgatcatga aaagataaaa aaccaacgtt
    48061 tcattttgat tccaaggaaa ggcatattga aagaagtaac gggtcaaaaa tgtatggagg
    48121 tccaataaaa attgtgtcgt ttgaaacaat ccaacaggaa gattgctata atctggtttg
    48181 gattttttcg tacagcgcat tcgcttcttc ataatggaaa gccatatttc aaatttcaat
    48241 cgtccttctt ctgtaatagc aaaactaaac tctttttgca gcagatctaa aaaagtgttt
    48301 cctgataaat cactgtattc ttttttgttt tcaagatagg gacgtccaaa ccaaaataat
    48361 tggaaataaa aataactaat ttgactttct ggaccggtca gccgcccgct ttttattttc
    48421 agatgaaact catttaaaga atcatttaat tccttcactc tgcgagacaa agtagccgaa
    48481 ctgatattaa gttccatgac aagtttctgc cgatcaaatg agccattttc caacagataa
    48541 agaagtatct gatattttgt agaatagcgc aagtaacaat gataataaac atcgactgct
    48601 gctatatttc catgtctgat taataaaata ttcccttctt ggataacaat ttccaattgt
    48661 ttgatatttt ccaagttagc ttgtaaataa aacagatctt ctttcaaggt agcccgatca
    48721 actgataagc gggtaagtac gttattaacg gaaacctctc ctttttcctc taataaaagt
    48781 tggaaaagtt tgatttgccg ttgttcattt ttttcaagca aactgaaaag ttccaagtcc
    48841 actccttcaa gcatttctga ttttaacagc agaagtttcc ttctcatcct tgaaaaagag
    48901 gatctgtgac agttatcaca gatccataca aattctaatc acttagctgg ttggcccagc
    48961 tgtcagttcc catgtgacca tgccttgata cgttgcgcca ggttgagcat tcgtactgct
    49021 atttacctct aagagtaaac catcggtatc gggaaagtca acgagaacct ctcctttagc
    49081 tgttccatca ctctcataaa tcaacgtgtt ttgaccttgc tgaatcaatt gctttgatcc
    49141 agaatgattg aagtataatt ttgttccgct taattcttga ccttgatcat tcttaaataa
    49201 agaagtcaat gtacctgtca gttgccaagg agacgtgctt ggaagccgag tatcccgcac
    49261 atcaaaagaa aaatcgcctt ttgattcata ataacttgtc ttcaacggtt tggggagatc
    49321 gttaccaaat gtaaaatcat ctggtacatt aactaatgtt aattcttgtt tggggacaag
    49381 aattgtttta gcagatgttt ggacaactgg gttaggaagc ttagcaccat ctgctaatgt
    49441 aatattgctg gaaagtacag ctgcctttgt cagtatgctg acggattgta aagaagtatc
    49501 tacagtattt aattgatagt tgtaagtaag atgaatggta tctcctattt tgctcaatag
    49561 atctgctggg acagaaactc cttgttgaat gccggtatct gcgatagttg taccatttaa
    49621 ttttaaggaa ccattgatat aagtagtatt ttcaggaagt gagatcgtga tttttttgtc
    49681 ggttatcgca tccccagatt ttgttaacgt gaatgtcaca ttttcttgaa tcgtatctcc
    49741 tgatagagca gacaggggat ctcctgtctc aggttgtctg ctaaggtttt ttagctgact
    49801 atctgcactc acagatggaa aattagccac tgttatttga ggaagagtgg tgattccagc
    49861 ttgttcagac tgccatttgt acgcaaaaga cggatacatg ttagtaagta catcgtccac
    49921 caagcgataa tcaaaataat attgccctga agccaatgca ggtagtgtag aagtagctgt
    49981 atttacaccc ccgttatttg attggatcgt ttggaaaagt tgatctgtcc atcctgtcgc
    50041 taatttttgg gttcctttcg aataaagcct gtaatggatc gaaccctttt ttagttcagt
    50101 tgctgaccaa ttggccgtga cttggtacga tgcagaagaa aggagctttc cccactgact
    50161 atcggctgtt aagtttgttg gtaaaaccgt tccgtctagc ttgtactgct ccaatacctt
    50221 taaagcaaca ggagaaagca atccgccttg cgcatctttc ccactgtaca cgatattggc
    50281 tgtctgattc aatccacttg tgcttgctgg agtatgagtc agtattgctc cttgtgtaac
    50341 gttttttcct gtaaaatagt tgactacgcg atcaaagcga ctaaatggca actgatcaaa
    50401 acgtccttca gtcagcccca tcgtcaatgt ccgactattg gtagagcctg gaaggttctg
    50461 gttatttcca tccacgtatt ctgtggtatc agcaaatgta ttatattttc ctgttgaatc
    50521 acttggtgag atgtaactat tccacgtatt tatctctgca cttgcagaaa atgcatatgc
    50581 tcctggattc aagtttccgc cagttaattt aggataaaat ctacctgcag ctggagctaa
    50641 aatcgaattg aacatacgat cttggataat attattcgaa tcataaaaat caacagaaaa
    50701 aacggccttt ttagacatag aaggttgtaa tttacccttg gtcaaaattt tgtttggact
    50761 cgtatcatat ttatctccgt tttctgtcat cttcgcgaca ttagctgtaa catcaaattg
    50821 aagcgggata tcgtccacgt tgaattcgcc tcgcgtaata atagcaataa tatctgtcaa
    50881 tagagtattt gtatcccacg accccatccc aaaacgcaag gtatggcttc ctgctgataa
    50941 taaacttccg ctagtaacct tgatgggggc accggcaata tacaagtagg cgttgttcca
    51001 gtcaattgct tttaatacat cactgcttga tacatgttcc ggcaaattga tgatcgccac
    51061 aaatgcaaat ccttgtgcag tgatattcac caaatcaagt acatttgtta gtttcacatg
    51121 gctttcccaa tgaaaacgga ccgtgccacc tttccaagga tcatttgaac catcgttcaa
    51181 ataagtagca gtcaatttag gcaattggtt agccccagtt agttggacaa gcttcccgcc
    51241 attgactgca ctacgtccac catttggagg tacgatattt tcatacgtgt atggatcata
    51301 agaaatggca ggaggcgttg ctccaacagc ttctgccgaa ggtgaaaaga taaatatact
    51361 taaaacaaaa agcgaagtaa aaaagaagga gcgaaaaaat gaaaatttat tttgtagcaa
    51421 catgttcatc ctccttttcc tttcttctcc ataacaacaa ccacgcaaga agccataatt
    51481 ctataccaat gatcaaataa agaaaacgga tcttttgttc attcgtctcc gggaatacgc
    51541 catgttgatt agattcttta taaatcggta gctgtgtctg ctgttttgat gcgccattga
    51601 ctgaaccact tggatgcgga attggactta gatcataaaa tgtaatcgta cctgaacttt
    51661 tggtatctgc ttggactggc tcttgccagc tgaagaacag caaacttgca ataatccctc
    51721 ccaataagat cgatgttttc aacgaaatca cctcatcatg ttgtttctcc ttttttcttt
    51781 ttcttgtgga cccacaaaaa cagcaaaatc aaccataaca ctagaactgc agcaaccaat
    51841 cctgcataaa gccatgggaa agattgttct tgtactgcaa aaacggaatt ttcatttact
    51901 ttttgtgctt tttctttttt gatcgtaaag ttttgtgtaa atcgccattc attactgtcg
    51961 cctgattttg ctatgatttc tgcgcggtaa tcgccggctt ttgctttatc accagctaaa
    52021 tcaagattca agtgaaaatt cgaatttggt gcaactaaaa atgcattaga cgtattttca
    52081 atataaagct gattttcata aaagatcttg ctcgtaaata tcaaattgct gataatccta
    52141 ggctgaggat tttggaaacg aagctgaaca gttggctttg cgttgacctc gtccaaatct
    52201 cctgataaaa gtttcagatc cggttctatc gtctcttgac tttcttgtaa aacgactgcg
    52261 agattataag caatacgatt tttgatttgt gctccttctg tagcattttc tgtttcagct
    52321 tcttctttag gtgaaacatg aatacccgct aatacacgtc ccttaaagct ttttgctggg
    52381 attttcactt ctataggtac agttgtttct gcatgtgctg atacattgat ttcctttttc
    52441 gcaatgagca aaacgtcacg gacatcaaac ggcaatgtct catctaacgt tgggtttttt
    52501 tgactataat cgatcgttcc accatcactc gttaccgccg gattgacaga gacctcaaat
    52561 gttcgttctt catctgaaga attgctgata attacttcta gtgtctgatt ttgttccggt
    52621 gccactaata aatcaaaata accgattgct ttatctattt ggttctctgg aaataccggt
    52681 ttgattccaa agtctcctgc tgaatccttt gcttggctga ttgacggaaa aatgaaaaat
    52741 agacttaaaa tagaaaaaaa cgaaacaaac agtcgtttac tttgattcat tgtccaccac
    52801 atcctttcaa caacaactgc ggtaaaaaat ccttcaccta tagtattttc accgcagttt
    52861 gttgaattta tttcatactc tatgctactg ggccagccac gaggttccaa gtcaaatcag
    52921 ccgtgaatgt tccagctgct gctttcggca ctttcagtgt gatatttgct ggatcataaa
    52981 cagaaagcca tgttcccaga ccttgatctt ttgctgctgt aaatattggt gtcgctgcac
    53041 ttgctttact caacccagtt acagtttgac tagttggtcc agtggattcg ttatctgacg
    53101 tacttttaac agtggatgtt ccaaaatcta attcagcgcc ttgcaaaact tgtgaaccgt
    53161 ttacaaaatc tgaaaccgaa actgtcacgt tccacccttg tgcttggcca tctgcaccta
    53221 cacctcgacg atctgatact tggagataag gtgtatcgtt ttttgaagcg tctacttgat
    53281 acgtttttgt tccgctttca atctcgtgtg taccaaatgg caattcggga actacatcca
    53341 gtgttaaagg acctgtctgg ccagttgctg gattttccgg atctgctggc tctactggtt
    53401 ttgaaggatc agtagggtta agaggctcca cggtatttgt tggttcagta aaggtaatat
    53461 ctccttttga agtcgcttga ccctcagcaa aagctggtgt tgccaagccg cctagaagaa
    53521 gtgttgaaag taataatgtc ccaatcatgt tctttttcat aataaatcac tcctttttct
    53581 tagtgaccta agtatagtag agacagtatg caaaaaatcc tcatgaattc atccgcttac
    53641 ttgaaattat gacccttttt gtataaaaga gaaaaaaaca ataaaatcgt ttacatgaat
    53701 attcttcctt ttttattaag tttcgtttgt caaattaagt tagacaaatc atcttttgtg
    53761 acgtaactca aaaatacttc cattggtgtc cgatagtgta atgattttct aggaatatga
    53821 tttcgtttcg atgccaccga ttggataaag gtttcagata caccattaaa atccatagct
    53881 ttatacaaac catctttacg taataagcca tttgaatgtt cattcaatcc tcgttgagaa
    53941 ggtgttcctg gatccgcaaa ataaattgaa atatcgttgg tattactaat ctttttccaa
    54001 tttgagaatt cttttccaca atcaaatgtg atggatttaa ataagttttt tgggacacaa
    54061 cgaaaccatt gattcagcct attttcaatg tctttagcct gtctgccttc gggtttcaag
    54121 gtaatgatga ctttagatag ccgttcaact aatgtgatga ccgcgctttt atgttttcca
    54181 cccacgatag tgtcaccttc caggtgacca aattcatgat caaactgggt atagtctgtc
    54241 tttctgtctc ggattgaccg gcgaaaccct tgttttcctc ttttttcttt atgaccgttt
    54301 ggttttcttt ttcccttcat tggtaaatcg tgagtgtcaa aggttttatc tttaaacatt
    54361 cgatacagtg tgcgtaccga acaagcaata ggaaactctg ctcgaccaat aaggacatca
    54421 ggagcccacc cttgaacaac ctttttttga atgtattcct gttgctcatc aggtaaaaca
    54481 atcggatgtc ttccgcagtt acgtttgttt ttcttatatt gtttatagta gctcattgcg
    54541 cttccaccag atttaagaaa acgatagacc ttataaatag tttgtcgaga acgttgtaac
    54601 agattggcta cttttgaaac aggttgtgat tgattgaaat aagcttctat cattacgagt
    54661 tcagttggtg taagatggtt ataggtcatt tgtactcact ccttataatt ctttcgtcgg
    54721 aactattttg agtgtagcac aaatgacttt ttttagttgt ctagcttaat tttacaatcg
    54781 gcgatttata aagaagaagc gaataagttg tgattcctag atttcgcgcg tgattaatta
    54841 atgagcaaaa aatgcggaaa gttttagtgt ttaatacacg aggacaaagt ttcttaggta
    54901 ttgaaggcac acgtgtaacg gttgatgggt ggtgtgattg tttcgaggtt ggaaaacatg
    54961 ttgaacttat ggcatttact gagctaaaag ataagaacaa cgtggaattt ttcgagagtg
    55021 atattggttg ggacgatcat gaagaagttt atggaaaatt tattttgcgg aagggaaatt
    55081 cttatacgaa tggggaagca taagtagcga tttgttagag atcattgata ggattgaaat
    55141 tgtcgggaat atttatgaga atctagagtt atttgaggtg atagaatgag tgatggtttt
    55201 gaacaagtat caataattaa aaatcaagtt cgggagattc ttagaaaaaa aggctatttg
    55261 gtagatagtt attttgaagg agattacgaa acatgggtag gtgtctatgc tcgtccagag
    55321 aataaaccta cctatttaga tcctacgacg tcagaagatg cctatttaca aaataggtat
    55381 cgagtagatg gttttaagca agactttgcg gagtggtttg aatgggaaat tgaaaacgga
    55441 gaagtaaaag aagaataaga gtacttgttg tatcaaaata agccagtaga acaaccaaac
    55501 cagtagctag taatgacaat aatactgagg aatcggatca ttatggtatg attgatattt
    55561 ctcttccaga agatcttaag ccagatcctg taacaattgg caatagtgtc atgatttttg
    55621 attcattaga tgaagctatg tcatatggtt ggaaagaaat taaggatgaa ggtagtaatt
    55681 ggtatggcat gagatttggt gatggtgaag ttgccaaaaa agctggtatt ccaattggga
    55741 aatggacagt tgatttccac gaagcaggtt ctttttttgt tctgtaatta tagtgggagg
    55801 gtaaaatcat tgatgaaatg tgttcagata agagcgccag ataaaaatag aagagacagt
    55861 tcacgtcgtt atcacttttg gtaacggcgt tttgttttat tatataaaat tctcaggaaa
    55921 aggaggaatt cttgtgtatg caaaaacgaa aatgaattgg ttgaaagcac tcgcattggt
    55981 ggctttttct caattctgtt tcttggtggg aactgctcaa gcacatgcag aagaaacgac
    56041 ggctatacaa cctacgatgg aagccattgc ccaagtcgct tctgaagtga cagaagtcca
    56101 accagcagaa gccggggtcc cagctggtga agccactgtt gaacaggccc ctgtgacaga
    56161 agcgccaacg gtcaattcca aaacgactct cgatcagacg attggggaaa cacaacaccc
    56221 agggaatagt cctggtactt cacaaccagt tgaagaaact gcctcaatta caacccctgc
    56281 ttcagaaacg tccactctag aaggagaaaa agctgccgaa gcaacgcctt ctgtgcagcc
    56341 agagtcttct gagaaacaag cggaagaaaa gaaacgaata gaagaaacag tggaacaacc
    56401 agcggctact gctgacaaag aaggaaaaaa tgaggaaaac gttgaaaaaa caatctcttc
    56461 aaccgcagct agtcaaaatc aaccaacgaa accaaacaaa gaatctacgg ctagtattga
    56521 aaatcaaaag aaaaaagagc agaacattcc aaaaacaggt acacctacac tgttagtaac
    56581 agtaccaacc aatcgttcgg ttcgtgttcg ccgtgcaacc aatgcagctg ttttaggtgg
    56641 tcggaaaatt gattggggcg aaaatattgg tgttacttct agtccgaaca atctaaactt
    56701 cactttaaat tatatggttc gttttgagat taatggacaa gtggcgtttt gtattgagcc
    56761 tggtgtctca gccgcagaag gtcaagttta tacagctact gctttgaaaa actatttgaa
    56821 aaattcaaat gtacggaaaa aaacaagctt gatctcttat ttaggatatg tcacaaataa
    56881 agacaaatcc gatgaacaat acattgcggc acaatttatg atttgggaag ctttgggcac
    56941 aaaaatccca atcaaaaata tccgtgttga ttatgatcgt cgaaaagcag aaattcaacg
    57001 tttgattaat aactttggtg aaaaagcaac ttttaataat caaacacata cggtaaaaat
    57061 tggcgaaacg ttgaaagtaa agaataaaaa tgattttttg aaatatgtga aagacattca
    57121 aaaaccaaaa ggtgtcaatg tatctattca cggcaatgaa gtatggatta cagtcacaaa
    57181 agaggcaccg gataaagcaa aaattcaatt gaataaaatt aaagttcaag gtgcgccatt
    57241 agcctatcgt cgtccaggag ctcaaacaat tggtgtgtta catccaacag aacctaatgc
    57301 gtctgttatt aatttacaaa ttctaaaaca aggaaacatt cgtgtgttta aagaagatgc
    57361 ggaaactggt gtgaaagcac aaggtgcagc ttctttagaa aatgcggtct atggtttgta
    57421 tcaagcagat gggaaaaaga ttaaagaaat taccttgaaa aaaattaacg gcaaagtaca
    57481 ggcagaaatt aaagatcttg atcctggcaa atatgtttta aaagaaatca aagcgccaaa
    57541 aggttatgtg ctttctgatg aagcaattga aattgttgtt gaacctggaa aaactgtcaa
    57601 tgcgaccgca aaagatcaag tgatcaaagg aaccattgat ttagtgaaag ttgctaataa
    57661 agatttagtc gattcaacga atccagataa caaaccaaaa ttagctggca ttgaaatcac
    57721 tttgacgtca aaaacaactg gcaaagtggt gaaaaccgtt gtgacagaca atgatggtta
    57781 tgctactttt ggtaaaaata ccgttgtctt tgatacgtac attctttctg aaaccaaagg
    57841 aaaagaaggg tataaactat ttgaaccact tgaagtcaca atttctgaaa atgggcaaac
    57901 tttccattat gttttagaag ataaagtaat tgaacaacgt cttaaagtgg tgaaagtgga
    57961 tcaagaaaca ggaaaagtga ttccacttgc taatacagaa ttcaagattt ttgatacttt
    58021 agccaatcgt tatgtcacaa tggagattcc aaatgataca gaaacaacgg atatctttaa
    58081 aaccaatgaa aaaggctttt tcctaactaa cggtacactt acttatggaa aagatcgcta
    58141 tcgtttggaa gaaatcaaag cccccgtaaa ttacgttttg aatcaaaacc cattagtgtt
    58201 ctcgattacc aatgatacag aaccgatcaa agtgatccat tttgcgaacc gtcaagcacg
    58261 gaaaagtgta aaattattca agtatgaaga atggaacaag aagaaaacgc cgctcgcagg
    58321 tgtgaacttt accttgtata acggtaaaaa tcaagcaatt ggtgaatata ccactgatca
    58381 aaacggggaa attttagtga aaggtctagt tgccgatgac tattacttct tggaaaatgc
    58441 cccattagaa agtttccaac caaatacaga taaacaagca ttcacggttg cattcaaaca
    58501 aaatgcgcca gatgatgggg aaacgttagt ggtttctgta cgtaattact tgattccacc
    58561 aaaactagaa acacaagcgt caaataaagc cgatggtgaa aaagtggttg atccattaga
    58621 aacgatcgtc attaaagaca gtgtttctta taccaacttg tttgtcggaa aagaatacac
    58681 catttcagga gtattgatga acaaggcaac gaatgcgcca attttacaag atggcaaaga
    58741 aatcacaagt accttggcct ttattgcaga caaagtcaat ggcacaaaag accttgaatt
    58801 tgtggttaat gctagtgttc tgaaaggtca aacgatcgtt gtttttgaaa aacttttccg
    58861 tgatggcatt gaagttgcag cacatgcgga tatcaacgat gaagcccaaa gtgtgaaagt
    58921 gaataatcca accgttcata ccacggctgt ctttgaagac ggattgacag tcgcagaccc
    58981 aacaggattg tttaccttaa acgataccgt tgaaattacc gagattcttc caggaaaagc
    59041 atacttggtc aaagggatct taatggataa agcaaccggt gaacccttgc ttgtgcatgg
    59101 aaatacggta gaaacgagtg ttggctttat tgccaaagca gaaaatgaaa cggtgatcgt
    59161 accatttacc tttgatttga caggtataaa tggtcgtgaa atcgtcgtgt ttgaaagtct
    59221 ttttagaaat gatgaagaac tagcaaacca taaagacatc aacgacaaag ggcaaacggt
    59281 acgagtaact aacccaagtg tacgtacaac ggctacacat aaacccactg gcttgaaaga
    59341 ggttaatcca ttaacgaaag tcaccattac agacgctgtg aaataccacg atttggtcgt
    59401 tggaaaaaca tacacagtca acggtgttct aatggacaaa gtaacggaaa aaccagtgct
    59461 agtagatgga aaagaagtca caagtacctt aacgtttgtg gcagaaacaa cggatggtac
    59521 agtcaatcta gactttattc tagacgcacg tgcgttgcga ggcaaagaag tcgtggtctt
    59581 tgaagatctg tttagagaag gcgtattatt ggcttctcac gcagatatta ccgacaatga
    59641 ccaaaccatc aaaatcagta atccaaccat tacgacgaaa gcgacgaata aaaatggcgg
    59701 aaaagagatc ttggccttgc caaatcaaac tgtgcttgaa tgggtcaaag tagacggtct
    59761 tgcgattggt caaatctatc gcttaattgt tcaaggctac ttaacaccag acggcacacc
    59821 atttgaaggc actcatgcgg aaaaagtctt tgcggccgat aaagaaatga tggaattctt
    59881 atttgaattc ttagtggacg gtcgcaatct tgcaggaaaa ggcttgagtt ttgcggaatg
    59941 gctccaagcg aaaacggaag atgagaaaga aacgtttgaa gaagtggcga aacacaatgt
    60001 agatctaaca aacaaagatc aaacggttca aattgtggaa gcgccaaaac caccgcaacc
    60061 agtgaaacca gaacaacggg ttaaaccagc tactgttgta ccaatgttgc caaaaacagg
    60121 tgaacaaaca agtagtatac ttatggttct tggattagta gtattagtca ttgctttagg
    60181 tataacactt tataaagtac gtaaagccaa agattagtca tgaggaacaa gcggttagga
    60241 tttttaaccg cttgtttgat tgtaatgtag aaatatttag aatttatttt ggtttttaag
    60301 aggaggaaaa actttatggc agaaaaagca ttgcgtatga agaaagagtt tttaggtcaa
    60361 gtatttttat tgggttcacg ggaacctaat ccaccaaagg aaattaatgg tgtgtctggg
    60421 cgatatgttc aattacaagc agagtgttcg cgagaagaac agcctgcttt ttttgtttat
    60481 ctcacagctg aagaggataa aagattacga ttaccagaaa aagaggtgcg tatttcattt
    60541 gaagaagctt attttgtttt tcgtaaagaa cgagtgaata gtcgtgaaac aaatgatatg
    60601 ggacaaacag agcgtcgaac aactggctat caagatgttg gtgtcttttt aactggtgta
    60661 aaaattgggg aggttttgta agatgggatt aaaatttcca aataattatg acattacaga
    60721 agcattagat gaaacttgtt taggagccgt acgctttggc gcattagaag agtctgatcg
    60781 tgtatttgat aatgaaacag gcgattatac gggagaaatt cgatcacagc gagcacaatt
    60841 agtttttgga aatccagatg aaccaaagat tgcacgacga gttaaatttc cagcagatgt
    60901 agatttgact ggttttaaac gacgtgatcc tgtgaaatta attgatccag aatatcatgt
    60961 ggtatatatt ccagcacaag atggcaatcg tgaatattca gatttagtga tttatgcgaa
    61021 aggattagag aaagtagaag ctccaacaaa taatcctaat atgaaacaat cacaagaaaa
    61081 aggtttagat caaaaacaac caagtgttga ttctaaggaa aaagagaaaa aataaggttt
    61141 ttttgaaaga ataggaagga gtcttcctat tctttttatc taagatagaa ggaaggggag
    61201 attttgaatt cagataaaac caatcaaata ctttgagaga ttgtgcttgt tatcaaaata
    61261 tccaacaatt gattgagtat tttggagaca atccaacgat ttttgtacaa acaaatgcat
    61321 atatttgtgc ggttattggg cagtgtatgt ttttacgtga tcttgttcgt ttttcattac
    61381 cgtaccagtg gaaacaagat catcaggaag tgatttggat tcgtatagta cgaatatgta
    61441 atatgtgtgc acacccttat aaaaatcgat taaatctttt tcgattatgt ttttttacta
    61501 aatgtgatat tcctgtatta gttgcttttt gtcaaaagtt tttagcacag gaaaagtaaa
    61561 aacagaaagg aggaatgtga caattgaaag ctcatttttt caagtatcgt ggtcggcgaa
    61621 ttcgctattt caatcgttat ttatggtttt gctattatgg tttctttttg tttccatttt
    61681 tgattctgtt aggttggttt agttggaaag tattttatcc acgtttaagt acgttttcga
    61741 attggcaaat gtatcttatt cctggaataa gtattgtgtt gtatttgttg ctggttagtg
    61801 ggttaatttt agggttgatg cattggtcgc gactaaaaga aggatatttt gcctctgttt
    61861 attggcgtca gttacttgtt cgaatgttaa ttgacaatcg gtttgtttat acaaagaaac
    61921 aagttagtga gaaacaaaca agggaaaaaa tgagattacc aaaagtttat ttttatcaaa
    61981 agaaacaaga attagtggtt tcttttcctt tagatggtgg gcgttttcac gaacgatttt
    62041 taaaaatggg tcatttgtta tctgaagtat ttttgcgtga tttaattcgt gaagaacgag
    62101 aaaaagcccg aatcaattat gtattccttt cagattctgg tcggattcct attgatcaat
    62161 gtatagcaga aaatggacgt attcatttaa tgaaaacttt ggattgggta tatgatcaat
    62221 caccaaatat gttgattgct ggtgcaatcg gtggtgggaa aacctattta ttgtattctt
    62281 taattcaagc ttgtttaagt gttgggacag tagatatttg cgatggaaaa gcggcagatt
    62341 tagcagcatt aggtgacgtc gatgtattca aaggacatgt gttttacaaa ataaatgaag
    62401 aaatgattat ttgtttacga aatgcattaa aagaaatgaa tcaacggtat gcctatatga
    62461 aaaccatgaa agatcctaaa tatgaaccag ggaaaaatta tgcttattat gggttaccac
    62521 cgcattttat cttttttgat gaatgggcag ctttttatgg ttcgttggat atggaagcga
    62581 gaaatagagt cgataaattg attcagccat tagtattaaa gggtcgtcaa gcagggatgt
    62641 acattatttt agcaatgcaa cgaccagatg cagaatattt tccaagtggt gttcgggata
    62701 atttaacatt ccgtatttct gttggtcgat tgtctcccat tggttattta atgacttttg
    62761 gagaagattt taaaaataag ccattttaca acaaagatca acctggtcgt ggatatgctt
    62821 ttccaggatt tgatgtacct gtagagtttt ttgcgccatt tgtttacaaa gattatcact
    62881 ttatcaaaga atttgagaaa tttactgaaa tgattgtcta tgattttaca gtaaaagagg
    62941 atagtcaaga tcaaacgatg ggagatgata atcagttgtt acaagaacaa ctagagcgcc
    63001 aaatgtattt agcaaaagat ccaaaaggaa caagtttttc tgatgcgcat attttgtaat
    63061 caactaagga ggtgaagtta aatggcagag agttgttatt atagagtagc tacagcaata
    63121 cgaatgatta atccttctct ttcttccaga acgttttatg attggttgaa tcgtattgaa
    63181 caagtaactg attaccgctt tttgcgcaaa gaacgtgttt ttacaggaaa agttattaat
    63241 caagtattgc taacgaaaaa agatatagaa cgattgactc gattgtatca ttatcgagta
    63301 gatttagaag aggatttaac tttgtcaatt tatcgcgttt tttctcctga aaaatatagt
    63361 gaaataacaa aattagatca tttaattttg tagttattat ctatcgggag ggctctagac
    63421 gtagtctggt agccgcactc tgcctggcgg tgcgcattaa ggctgcgaag catgggccta
    63481 taatgcgcac tcgctagcag cgtgtgcagc tcacaaagag gtcgtgcgct tgcgcacggc
    63541 ctctttgtgt aaagcccccc gatatataat atggggggca gaaataacat aacacataga
    63601 aaaaagtttt ataaaccctt ttgtatcaag tgattatcta aggtcagcag ttgtactact
    63661 tttcccaaaa aacgaggggg actagttgat atatcggtgt ttctaaaggg ttaaaaaaaa
    63721 tcgattttgt tattttataa aaggagcgag aaaatgacac cgattgagct aaaaagctac
    63781 agaaagaagt taagtgcaat tcataatata agtggagata aaaagccgct atcacaagca
    63841 agatttgcgc aattgatggg ctgctcaaaa gattacatta gaaagatgga acaagggaaa
    63901 agaaaaatcc cagaggattt tgaacaacga atcaaggaac gctttcgatt gtatatgaaa
    63961 agcggaatag atcttcgagc aatgattgat tatttgcgtc tttccttttt tgggacagac
    64021 tacaaaacag tgattcaacg tgtattacga atggatctga cagaatttga aacacgtcca
    64081 acgggcttgt atctttttga ctgtctttct gttcgtggga atatctgggt gttctggcat
    64141 tcaaaacgaa ctgtaacaga aaatgtactt gtgcaatttt caggacaagg ttgccgggaa
    64201 tatgaggaag tcatgcgtga acaacaaaca gattggcgaa catggctata ttccatttgg
    64261 aaaaatcaag gaaagttgga agctggttat acacgtgtgc aatgtagccg attcgatatt
    64321 gcgttagatg aactttgggg attggaaacg gaagaacatt tcgatctctt tgaattacta
    64381 gaaaaacaaa aagcaggttt attagaaatg gactttaaga gattccagaa tattggcggt
    64441 tgttttttag atgatggcta ttttcgaagt gaaggattat ctttgtattt tggatcgcga
    64501 aagagtcctc tcttttttaa tttttatgag aaacgttttg aaattgcgaa ccgagaaaaa
    64561 atttcagaat tagaagcatt aacgaaatat ggtatttata atcgatatga gttacggtta
    64621 gccaatgaga aagcaacaca agcagtagaa gcatttattt taggtgattc accgaaacgt
    64681 ttaggagaga ttggcgttgg tttaattaat gcaaaactta ttgtttggga tagactggaa
    64741 agtgatgcac ggattgttga taaacgatgg gcaaaattat ttcgttcgat ggaagaattg
    64801 gattttacag taaagccaga aaaattctca ttagatcgtt cagaacgctg gtttttgaaa
    64861 caagtagctc cgacattaaa gttgttacaa gaagcagatc gtattttagg aagagacttt
    64921 gtacaagaag ggattgataa tgccgaacta aatgacgagc aacaaaacta tctagaatgg
    64981 atgggaacgt caggagggaa aattgcatga atgcatatac gacaacagaa tggctgcgcc
    65041 tgttggattt aaaggctgca atcgaagcat taaatgaaaa gatggttgat ttgtcttatt
    65101 ttcgttttcg agttccttat attgaacaag cggtaaaagc tggacgttat caggaaaaag
    65161 agaactggca agaaattgca cgcttattag aagttcgtaa gggatacgaa caagaactcg
    65221 aagagttgga atttagccga aggaaaggaa ggctagaatt cattcgcttt tatcgatttt
    65281 cattgccaat acctgcgatt ttagcggtta aaaaaggctg cgataaaatg aagatatatg
    65341 aaaactgtgt ggcagcactt agcagcgaaa aacctcttat ggaagaaatt tcattggtga
    65401 ctgtcacttg ggaaatgagt cgccagccaa cagaagaaca gacatatctt tctttagaag
    65461 agattgagat agaattagag gaaattggtc gctatgccac ttgttctacc tattgtggat
    65521 cggtcattag tattgctggt gtgattgtct aaatgaaaaa aattatgatt tatgttggtg
    65581 cgtattggtc acgtgatcca acagtattag aaaatccaga ggcggtacac tattgcttgc
    65641 gccaattatt ctatctgtat aaagaacgat tagaatcgtt gattcgtcag ttaccatata
    65701 ctgacagacg tttagatgaa cttcttctaa ggtatccagc aatgtataaa cgacgaaaaa
    65761 atagattgtt accagaagag tatcccattg aaaaacatga acttgaaggg cgatttgtgg
    65821 cttatttcta tgatgatgtt cgtatgcgct tagtagaaca acgaatggaa atagaaaatg
    65881 atcgctacta ttttatcaat tattgtaaga agcgtaagta tcaagtaact gatgattttt
    65941 atgattgtat tttacaagat ggggaagtga ttctatccaa aattgctcca agttttcgtg
    66001 aacttgtgcc gattgatttt ttgaaaaaat gtcatttgcg gattcttccg taaaaaagag
    66061 ctataaaagg aaagaaaagt agaagccaaa tcggtttctg cttttttatt ttttttagga
    66121 aggagaagat ggtatgtcac tggaattttt aggagagcca attgaaaagg aagagttcgt
    66181 ttcgcattat atggatttgt ttattcaggt gataaaaaac tttgtgcgat tgatgaattt
    66241 tatccacgcg aacaagacta tctagccgct gaacgaataa aaatcagttt attatgggaa
    66301 aaattcacag atgaaaataa ccgaccgcca gattatcgct atttatcaga ttgtttaacg
    66361 aataatgtgc tttctaaaga atacctattt cacaaacgtg agaaaaatca aatgtcggtt
    66421 gaacattttt gtgattgtta tattgcatta ttgcgtagtc aacagttatt aacagtagaa
    66481 cgatatggtg cagagtttgg ttatgtcatg caagctgagc gtcatagagc tacaagatgc
    66541 tatgaaaaag aagatcaagt gattgatggc tatgaagaat tatgtattcg aaacaatcgc
    66601 tatttgcaac aacgtttata tgaacaattg atgatggatt tttcaaatct ctatagaggg
    66661 tatgaatgtt cgatgattca agaaaggtag aagaaaaggg ggaattttta tgtggcgatt
    66721 attatttttg gtggtatgtg gtatggcatt gttctatgtg tatcgtgtct atctctatcc
    66781 acgaatggaa cgatggacaa gttggtatag agatccttta aaacaacgaa aaggattgaa
    66841 gcggtatcgt tatgaattac ttgttgaatt ttcggatggg cgacaagcac agtattattt
    66901 ttttgcaaac tatccaaaat acaacaccag tgaactattg attcaattat tcaaaaataa
    66961 tccatatgtt gggttagaag aaaacggacg ggagttcttt tatcgttcag ataatattag
    67021 tgcggcgtct atgaacaaaa cacgaattgc ataaaaaatt ggaggaataa tcatgaaaaa
    67081 aacaacatta gcagtcgtaa caacactagg aatttcatta ttttcgtttc aattagggag
    67141 ttcattggtt tatgcagatg aaactgtaga aacacctaag tcagaaattg tagaaacatt
    67201 gccaggggaa ggattaccag atgttgagac agagctaaat ggaggaacaa cggaaaaacc
    67261 agcagaacct gaacaattgc agtcagagaa acctgaaaaa gagccagtga caccagaacc
    67321 agagtttcct gaatcaactt taccgactga ttcgatcgaa gaagaaggtg tgcatagttc
    67381 aacaacaacg gattcaagta tagtcacaac tccggaacaa tcaggaacaa ccagttcttc
    67441 tgaaaagaat actgattcgt caagtacgga acagccaacg atgtcgtcta ccagtgaaga
    67501 acaaaaagaa gaaccgaaac aacctgttga aaaatcagaa gcaattccaa aagaaaataa
    67561 acctattgtt ccagcaaaag aagtcacagt atcggtaacc ccaagcggtg aaattacgac
    67621 gaatacttct caaggaatga gtgtaccgat tgtcactagt aatgtagagg aattgaccca
    67681 tattccaaca ccaaccaccc cattaaaagt agcaggtgat caaacaattg ttggtgtaaa
    67741 agatggtatt ccattaatcc aagatagtga agggaatttg gtaaaggatt tgtcgattcc
    67801 tgtaaagaaa cttccaagtg gcaatattga aatcaaaaca gcagatggaa aaacaaaagt
    67861 cttaccaaag actggtgaag aaatacatgt tgcactttct gttctaggag gagtgttgac
    67921 aagtattgct ggatttgttg gatataaaag aaagagaaaa ggaatagaaa atgagggtaa
    67981 ttgaatggac tatttttcag aaaaacaagc ttatcgttgt caatcgtacg tactagtaaa
    68041 aacatactag aaggagtagc ttatgaaaaa acgacaacta cggcggaaag aaaaagttta
    68101 tacagagaaa gtaccgaagc aaaagaaaat ccattcaaaa aaagcagtga aatggatttg
    68161 gtgtttggtt ttatttcttt gtttttcagg gggcttggca ttttttcaat ccatgaaaac
    68221 gaaggttcaa gtgtcgtttt tgaaagatga aatccaacaa ttacaaaaga aagaagtaca
    68281 aagtcggtca gaattaacgt acacacctga aattgaatcc tttatgaatc gttttgttgc
    68341 tttgtatatg aatacattcg ataatcatga acaagaacaa caacgtcaag aaacattaaa
    68401 aaaagagttt tatgcacaaa actttattca agaaccagga agtgtttcag gtgttcgaaa
    68461 attaactgct gcttcgttta ttggattaaa agaaattgaa accattccaa ctgctagtta
    68521 taaagtaact tatgatattc gctcgatgaa tccagagagt aagaaagaaa caagtgtgac
    68581 aatcacacaa gtactcaatc ttcccttttc tatttcaaac aagggctgtc gtattattgc
    68641 ttatccttat tttacggcac taccaaagaa tacagaaaaa caacgaatga ttacttatga
    68701 tgacacatta tacgaaacgg ttgatgcgtc ggaagcgaaa gatgttgaag gatttgtgga
    68761 agatttctta gataagtatg cgaaaaacaa tgcaaaagat atgtcataca tgatgaaaga
    68821 acctgaagga ctcaatcaaa cagctaaagt tgatcaagta gagatgaaag tattgcgtta
    68881 taaagaaacg aaaaaacctt ttattgtaaa agcaacggtc gtctttcgag atggtgatac
    68941 gtcactttta caaaaagaac aaatgacgat tgtcattacg aaaaaaacaa atcagtatta
    69001 tgttgaaaaa ttaacacata attggttgga taggaaagga gaagaacgga aatgacactg
    69061 aagggaattg tagattttat attaacgcaa ggtggctatt ttttaataat ggtggttgtt
    69121 tatctgggat tgcgaaattt tatgaaatca aaagtaggaa atgcattaat atctatcgca
    69181 gcaggtgccg cagtttattt cttcttaaat gatccaacac gtgtatttga tgcgtttggt
    69241 aaaatcattg aaaaattatt tatggattag gaggatagtg aagtggattg ggaaccttat
    69301 gattattcga ttgtttatgc ggaaccttat cgcgtacaac agattacaag caattggcgt
    69361 cttccatttc cagttgcatt agtcaatgca gcaacttttt tgttggtcgt aatctttgaa
    69421 tatttatttt tacgttcatt tattggttgg gtcatttctt tttttccacc agcaggagtt
    69481 gttttttata taggactccc ctatttatgt gtgttagcac tggcaaaact aaaacctgat
    69541 ggaaaaaaag tgcattttta tttatgggat tggttttgtt atatgattcg tatgatgaat
    69601 ggtgattgtg tgtattataa ctgggacaag gtatcaaaca atgcattgga agaattactt
    69661 tttgtgaata agtatgttta tttagaggat tagaaaaagg gacaatcaac tggttgttcc
    69721 ttttttattg ggaggtgaaa ttcattggtg cttgtaaaag aaccagtaaa agcattcaag
    69781 aaaaattgtt tgttaaatgg agaggattgt atctgggcct actatcatgt ttttccacga
    69841 acaatttcaa aaaacacacc aaaaaagaca caggaaataa aagaaatgat ggctcgttta
    69901 ctgttgcttt tagcagatta tgaagatgtg gagttggtga tgtatccacg aacaatgaat
    69961 ttacggacga agtttgacac attaggaaag ggattttctc ccgatagtca agcaattggt
    70021 gcgtattatg caaaagaaac agtgacattg ttggaacgag aaggtcaagt ctatcgtcct
    70081 tcatttattt taggaatcaa agtcaaagaa aaagcaacgg tggagagttt tcatgatgta
    70141 ataaatctaa tctggttaga tttagcagga agtttagcac gcttatttgg attagaagta
    70201 gatgatttgg aaactttgtt ttctgaaagt gatccttatg taaaagaagt gacacaatta
    70261 ctttatagct tttctattcg tcccttagta aaaaaagagc tgttgtatct caatcgtctg
    70321 caatatattc gaggaatggc tcacaaagta gaagaacaaa ataattggga agtactgaaa
    70381 gacaatcaag gaacgtatgt acgtataccg aaggaacata aaggccttct tcaattagaa
    70441 gatgagttag ggagtagtta tttagcattg ttgcctattg gggaaacacc tgaaaatatg
    70501 agaaattgtg atctatttca atttgctcag gatctttctt tcccagcaga attgcgaatg
    70561 aaaattcact atccacgctt gaaaggacag ggaggtattg gtttaaaact gaattggcta
    70621 tcggaacggt tcaaaacaga gcataaggaa ttggttcaaa atgatgaaga acctagtgaa
    70681 cgcttattaa ctgttcgagg attagtaaaa catttaaagc aacgagtaga taatgggcaa
    70741 cctgtcgtcg attggatcgc atgttttatt gtatacggtc aaacggttga agaagttcgt
    70801 caaaatagtg caacggttat tcaacaactt catacgatta tccatatcca ccgtgcaaaa
    70861 aatgatcaac gagaattgtt ctataaatgt ttattagggc aaccattggg acggcagtta
    70921 cattggaagc accgaacaac tgtagaagca gttggcgaat ggttgttttc agcagtatca
    70981 gaattaggta tggaatccgg ctggtatatt ggtcggcaag atacattagg ggatggtggt
    71041 tctcgaggag aaaaaacacc attagaagaa ttaatttatg cgtcaaatcg ttttgtcttt
    71101 ttgaatcttc tagccattgc ggaaggaatc aaaggtgcgt tgtttgatgc gcctcatgta
    71161 gcagtaactg gaaaaaccgg taaagggaag acctttttag ttggcttgct ctttttgtat
    71221 agttcgttta tgaatgtaca aagtttattt gtggatccca aaggagaaaa gcgcaaatgg
    71281 tttacgaaac tcattcaaga cccgtattat cagaaacatt accctttgtt tgtagaacat
    71341 ttgcaaaaaa tgagttatgt cacgttagat gctacaaatc cccgtaatta tggtgtatta
    71401 gatccaattg tttgtttatc ccgagtagat gcgaaacaag tagcccagga tatgattgat
    71461 gagttaagtc cgttaggtac caatcacctt ttaaaagggg cggtattaaa gggaatccaa
    71521 gaagttttag ttcaaaaaga agccggacaa caagttggct tgatgcatgt ggtcgactat
    71581 ttagaacaaa tggaacaaaa agaggttcga gaatatggtg agtttttacg cttgacggta
    71641 gaagattcga ttttacgttt aggattttct tatggagaaa acccgggatt agactttaat
    71701 gccaaaacaa cgattttaga gatccaagat ttgaaattac caaatgacaa tgtccgacca
    71761 gaactatata cagatgcgga tcgaaagtct ttgtgtttga tgattagttt aggtcgtttt
    71821 tgtgaaatgt ttggaaaacg agatagtagt aagaaaacag cgatttattt tacagaagca
    71881 tgggtattta ataattcgaa cgcaggacgt agtattattg cagcaatggc acgtgtgggg
    71941 cgttcccaga tgaatcaatt agtgttagat acacaattta ttggtgactt aggttcagat
    72001 aaagaaaaag ggaattttgg tgtcgtcttt gcttttgatg aagacagtga gcgagaaaag
    72061 attttgaatc atttacgttt ggaagtaaat gaagcgaact tagctgaaat gagccacatg
    72121 attaaaggac agtgttggat gttagatccc tatggacgtg taggaaaact caatgtacac
    72181 agtttgtttg aagaaatgac cgctgcatta caaacaacgg aaaaaacaag tcactctcaa
    72241 gtagaagaac aggatacgtt aatcatgcaa tagaaatgag gtgaaacaat ggcttatata
    72301 aagcataagg aaaagaaatg gtgcgttttg ttttttttaa cgattggatt cggactgctt
    72361 tttgttggtg gattggtgta tgcggcagaa aagcaggtgg atcaatcgat ctatgagatt
    72421 gggaaatttc atattgatac cttttcttct tatttatctg atagtgaatt ttcattaggc
    72481 aaaatgttat ttggggcaaa tggcacagat tttctaaaaa tgatgacgaa tatgttcttt
    72541 agtttaagta aattgatttg gcaaggcctt gattttgtca ttgagaagct ctatgaagga
    72601 gctgcaatga acgagttaat tcaccagttt ttcacttttt cggatactat ttattcaaaa
    72661 atatacgatc ctattggtgc ggggattgtc gtcttgtatg tgttatatct atttactctt
    72721 cgtatattta gagggaaaca ttacgcaaaa atggtgttta ttcgatttct cttcgttgtc
    72781 attttttcaa tggtttggtt tggctatggt agtaacgtac ctagtaaagg agaagctttt
    72841 accaaagggt taaatgattt gtccgtggag gtagaaggct ttattttttc tgctacaaat
    72901 agtctagatg aattggcgac aaaagatgtc aatggtgttt tagtgccaac gacacaaaag
    72961 gaagccattc aaaaagtgcg ccaactttat tataaagcgg ctgttgttga tccgtatata
    73021 ttgttaaatt atgggacaac aaatttagaa gaattgtatc aagcagagat tgaaccacaa
    73081 gaatttctag ccaaggaagc gtcaagtgaa gaattatctg atattgagac aaaagtgacc
    73141 actgctgcag gagaagatag agggaaaaag aaggcttatc gtgtctatat tcaaccaact
    73201 aaagctgttt ataaagcagt cgttggttct atggtgccat tgcttaatct aagtattgga
    73261 ttaccacttg tagtgattgg catgattcgt tttatctttc aaatgttggt gttgtgttta
    73321 ttacttgtga ttcctttttc attactcgct tcttttgttc cagtatttga ttatatgctc
    73381 atgaatacct taaagtcctt tttgggcttg atgtttcaaa aatcttttta cagtgtgatt
    73441 attttggtgg catttttgat ctataacata gtagacgatt taattccaat gaatactgtt
    73501 tttggttttt taggaaatat gtttgtcaaa gcactcctat caattttact cattgtaaaa
    73561 cggaaagatt tattaaagaa actagggtta ggagctgcag atcaaactat aaacaacacg
    73621 aaaaacatag caagtcattc tttgtctcaa atgaaacaac tgcctggtca actatcaact
    73681 gctggacaaa atttggcaat caaaggtgca ggattagcag ggaatgtgta tcgagcaacg
    73741 aatccactcg cttctgcagc gatacatgga tttcaaaagt ttcaacaaaa acgtacacca
    73801 caagaatcgg taaaagggag gcaagtggtt aatccctttg aactaggtgg gcgttcaaat
    73861 caagccttgc cttatagtgg tcgtcatgtt cgttatcatg gggtagagcc tgaaatgtct
    73921 tctaccaaat cgaaaacaga gtggaattct ggtctttcaa atggtttgat tccaaattcg
    73981 tccatgaatt cagtaagacc aaaacaacag gtacaatcat tggcacaatt gagtaagcaa
    74041 caagggaata atcaaagaaa accattgtta aataaaggta tcgcttttgt tggtcaatcg
    74101 attccgttgc cttctggaaa agtacccgtg ccacaattaa cagctgctgg ttctctttca
    74161 caacagattc gccaagtgaa aaatcaggcg aaaaatggga gttatccagc aatgcagcag
    74221 ttattgaacc aatactctgt tcctaattcg tcggtagcca ctctatcagg aaatcgttct
    74281 gtgcaacaac cggggcaacg ttcgtctgaa agtaaaccgt cgatttccgc accaaaatcg
    74341 ttacaacctg cattttcttc tttagataag ccaacagagc aatcggaaaa aatagaaaag
    74401 gatcctaaaa cactgccgat tttggacgca aaaccaggaa ctgattttac tttaccaaaa
    74461 ggacgaacag tccaaaaacc attatcgaag aaaggatgag gaagaaatga agaaagggtg
    74521 tctaggttgt cttcttttct ttattcttcc cttttttcta ttgccattct ttttttcagg
    74581 agaactttct gagcatggag gagaagctac aggtctagct gtcgctttta atggggaata
    74641 taccgaagga ttaccgattt tttcagagat taaaggacgt ggtccaatca cagatgaaca
    74701 tgcgcaagca gcagttggtg cagctgtaaa gtatcagtta ttaccgagtg tgattctttc
    74761 tcaattaggc tatgaatcag cttatggaca gtcacaatct ggaagaaacg atacgaactt
    74821 ttttgggatt acctggtttc ctggttgtcc ctacccacaa ggttctgcac gtggaattgg
    74881 tggttctgaa ggtggtttct atatgaagtt tccgaactta aaagcttgtt atgactatta
    74941 tggctttatg gtggcgagcc aaagcaactt taatcaatgt gtgggcaata aagatcctgg
    75001 aagttgttta ttgattctag gtcgtggagg ctatgcggca gcaggtatta ccgaagggag
    75061 tccgtattat cgaggagcaa tgagtatcat aacgtcctat aacttaacgg aatatgatac
    75121 ttttgcgatc aagcattgga atagcctacc aacaaaaaaa acttcgctta ttgcaggaaa
    75181 aagtaaaggc aatgcgaagg ttttggatcg gatcattgga cgatccgtaa ataatgggga
    75241 atgttatggt ggtacggcat ggtatgtatc tcaattaggt ggcccacagt taatgggttc
    75301 tggacacatg tttgcgatgt taatagggga agattataac tggacaacct atggttggca
    75361 agtaatcatg aacccaaaac caagtgattt acaagcagga gatgtggtga attggcaagc
    75421 aggtggtgca ttgtctcctg gaatctatgg tcacaccgga attataacag atgtttcata
    75481 tggcggacaa catttctcta gtgtggaaca gaatgccgag caaggacgga ttatggctcg
    75541 gtatcaacgc acgtacaatc aaacaaaaat tcgtagtctg gttcgtaagg tgaaataaga
    75601 aaggcagtga ctagaaacgt gaaaaaaggg ataattttag tatttagttg gttatgtttg
    75661 ataggtctta ctggttgtca ttggttttct tctgaaaaac aaaaaacaca attgagaacg
    75721 acagaagaaa caatcgaagc agtaaaagaa acaagtggaa ctcttcaaag tactgaaagt
    75781 aacagtcaaa ttggttcatt agaagacgag acagcgcaac aaatgcaaat agatgaaatg
    75841 acaaaaaatt tcttaaaaga ttttgctaga aaatggagta atttcacaga tgtttataaa
    75901 cgcaatcaag ctgtgaggga ctatatgaca gatcgttgta ttaaagacaa tagtattgat
    75961 gtagacccgc atgtacaaat gaaaacaact gggaaacttt accaggtgac acaagatata
    76021 gagaatccgg atcaatattt gttatttgga gaagaaacaa tgaataaggt tactcaattt
    76081 gtattacttc aattgaaagt agatcaagaa gctcaaaaga ttgatgcgat caagatttat
    76141 tatgtacgga gtgcgtatta aaataaaaaa tgaacactca tatttattat acaccgaaga
    76201 aatagaaaaa aacagactgt ttttttaaga aaaactatgt cattctttac ttgaaaaaag
    76261 ttgttatagg acagaaagga taaaacaaaa acggaaaaaa tatgtcttat gaataactat
    76321 aagaactatt ctatttttta ggagatggat acgagatgaa aaaactaatt ggctatagtg
    76381 tgatgtgtat tggatttgtt ggtgtgggat tgtttgtatc gactgaatct gcttctgcgg
    76441 ctgaatggta taatcctatc tggtataatc cgaacattga atgtcaaaaa ggctatccgt
    76501 atggaccgac aaatctgaaa aatggaaaat gtagagtaaa ttggaatggc gttgtagata
    76561 gtgtaattac tggttcaatg gactctgctc tacatggctt tacgggtcac tagaaggagg
    76621 aagaaaatgt ctgaagagaa aaaagaaatc gttgaatcat tagtagcttt aaaagattct
    76681 ctttctaaga ttgaatatgt tgataggcaa gaaattcaaa aactaattga tgatactatt
    76741 attgaaatcc aagatgcgcg ttgtgaaggt ataaaaattt ctgtagcttt atctaaagta
    76801 atagaaaaaa tgaaccgttc attagcgttt aatggattga agctagatag acaaactagt
    76861 ctgatatggg atcatttaaa agacttgtat gacaagtcta aaagaagtga acggactgct
    76921 gtatcaatct taaaaggcct ttggggaatg aattcataac ctgaataatt catactttta
    76981 attaaaacca tgtgaaactg cctcacggca gcttacaatt actttttcat cttcacccgt
    77041 taagtgatga aaaaagaacc cctttctgct atggttaaag tgactaaacc aaccaaaaga
    77101 aaggagttct tttcatgact agcttacaca aaaaccaagt aaaattcaat tcaaatatca
    77161 ctatttctca tacaggtggt caattatcga gtgattcggg tctcgtccta gtgaaagagc
    77221 tgatgaacat ctttggtttt tctgaactgg ctaagcaaca cattcacatt gaagacgaac
    77281 gagcgtattt tactcatgac aacttatcca tacttgagca gttcattatg caattaattg
    77341 ctggttactc agctgattct gcagctaatc tcctgagaca agatcctgtg tttaaagctg
    77401 ttttagatag gaaagaactc gcttctcaat cttcactttc ccgattttta gatcgactat
    77461 ctgaggagaa tatccatgaa cttcaagctt tgaaccaaga acttatcgat aaagcacgcc
    77521 tcatccgtaa tgatacggaa ttaatcattg atttagattc gacccattca gatacatttg
    77581 gtcaccaaga acaaacgaat tataatacac actaccaaac ctatggttac catccattgg
    77641 tagcttttga cggattgact ggtgacttct taaaagctga actacgttca ggaaatcaat
    77701 atacatcaaa aggcgtaaag gagtttctca cacctttatt agagcactat aatcactctc
    77761 taccaaacac tgacatcttg gttcgtggag acagcgggtt cgctacacct ggtgtgtatg
    77821 attcgtgtga atcaaaaaag agtcattatg ttattcgact gaagaataat cgtagactag
    77881 gtcaaatagc tgagaaatca gtactttatg gcgataatca aaagtgggaa gaacgagaag
    77941 ttcagtactt ctcgaccact tatcaagcac aatcctggtc acaaagtcgt cgcgtgtgta
    78001 tacgctcaac acgtgaagcg ggcgaactac tctttcgaca tgaatttatc gtgacgaatc
    78061 tatcagaaaa tgtttctcct gataccatct tttctatcta tgccaaacgt ggcacaatgg
    78121 agaacttcat taaagaagcg aaagctggct tttactttga caaaacagac agtcctcgct
    78181 ttttggagaa tcatgtccga atgatgctca gcttgatagc ttacaactta gtcaacttct
    78241 taagaacgat tggctttgaa gaagtccaaa agggaatgac cattcattct attcgattga
    78301 agtttctgaa agttgctggg aaattagtcc aaacgggtag acgagtctat ctcaaattat
    78361 ctagttatca tgtgtatcag aatgaattct acagggtctt tgctcgcctg aggcgagcca
    78421 gtcaatggat ctaatccaac aatctaacga tttttttact gggaagttat ccagggagaa
    78481 gtatgctcaa aatgccttac acccaaaatt tattcctcaa tatttttgaa cagtgaatgt
    78541 ttgaagtaac tttttagtca aaatcagttg atagggatat tagtgataca tatttcaata
    78601 aaatgtgcca aagaattaaa gctatgaatt attcaggtct aaaaagcttt ttaaaagtga
    78661 tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat ccaggtctgc
    78721 ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt atacgtatgc
    78781 aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga gtagtcaaca
    78841 gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat gtccttcacc
    78901 aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt gtcataataa
    78961 atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct aatacgattt
    79021 cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca gttgaatcgc
    79081 ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc ctatagaatc
    79141 ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct atcaaaaaaa
    79201 atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag agtcaaagaa
    79261 aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag ttatactttt
    79321 tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg acgaatacgt
    79381 gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag aaaagaatga
    79441 aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac tggacaggat
    79501 ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc ggtcaaaaaa
    79561 tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa cgtgcgattc
    79621 ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata ataaaaacat
    79681 ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat aagatgaaag
    79741 aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa gttccgccgt
    79801 taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat ttgatcccat
    79861 acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa gatcatctag
    79921 caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat agcatccgag
    79981 cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc gatatcccaa
    80041 atgcacaacc aatccagtat acattagcag atgatttgac aattctggac aaaaaaatac
    80101 tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag gaatggaaca
    80161 aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat acgtatcccg
    80221 caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg gacaaaaaag
    80281 aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa tcaaacttga
    80341 atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg gaagtttccc
    80401 gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc ggtcgtcatt
    80461 tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa catcgattgg
    80521 cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa cctgatccag
    80581 catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg atgatgatcg
    80641 gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact tgtctttacg
    80701 acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat ctgaatgaaa
    80761 tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag atatgttata
    80821 attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga agttgtagaa
    80881 gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt gaaagtaggc
    80941 ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat cgtaggagca
    81001 cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt gagtcagatc
    81061 aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga agaagtggac
    81121 gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt gacagagatc
    81181 gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga atagacagta
    81241 aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta ctaattgtaa
    81301 gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact gaaaaaaatt
    81361 ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc gatggacttg
    81421 tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta gtggttctag
    81481 tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca aaattttcaa
    81541 cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt caattgatct
    81601 ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt gtaggtcaag
    81661 gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg atcattggtg
    81721 cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact gtattgactt
    81781 ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca atgctgttcg
    81841 gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc tctgaactgg
    81901 caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta tcctttatcc
    81961 ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt ggagtcgtta
    82021 ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg ccaaaagaag
    82081 cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg atcgtgacgc
    82141 ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt ttggcaacaa
    82201 gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac gtagcattgc
    82261 ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat ccaatcaact
    82321 atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca agtgctggag
    82381 ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat gcaacagcta
    82441 atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc gtttatccag
    82501 ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt ttggaattta
    82561 ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga gtcggtgggg
    82621 gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta gcaatcgttg
    82681 ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta aacgtcagcg
    82741 gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat cgcgatgtca
    82801 tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt tgtgatagaa
    82861 gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa atttttgtga
    82921 aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag gttgtgaaaa
    82981 agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttacgt
    83041 ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggtcgtg
    83101 agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt ctcatgtttg
    83161 ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac cagaggttgt
    83221 gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca cattatttat
    83281 tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt aaagaaaagc
    83341 gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt tcctatggta
    83401 aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat gtttacgaat
    83461 gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt ttggagagca
    83521 gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat tgaagtagga
    83581 gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga gtcccattca
    83641 cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat ctttggacta
    83701 tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct tggagaagaa
    83761 tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg taaataatag
    83821 aaataaagac tttgtttttc tgtgcgaaac aggaaagcga agtcttttta attcaaataa
    83881 taatattatg taaacaaaaa actttccatg cgtaattata cacaagcaaa taaatcgtca
    83941 aatgcgaacg ccttctttat actgtttagg aacgaaaaga aggaaggaag aaatacatgg
    84001 attatcaagt actgctttat tacaattata caaaaatcga agaaccagag gaatttgcca
    84061 aaaatcattt agccttttgc aaatctttgg atcttaaagg tcgtattcta gtagctgaag
    84121 aaggaataaa tggtactgtg tctggaaccg ttgaaaacac gactgcctat atgaaagcaa
    84181 tgaaagctga tcctcgattt gctgatacga tgttcaagat cgatccaagc gaaggacatg
    84241 cctttaagaa gatgtttgta agataccgtc cagagcttgt aagtttaaag ctagaagagg
    84301 acattgatcc aaaccaagtg acaggtgcct atctttcacc gcaagaattt aaagaagcta
    84361 ttttagatga acagacagtg gtgattgatg cacggaatga ttatgaatat gacttgggac
    84421 attttcgagg ggctgtacgc ccagatatac gcagttttcg agagcttcca gactggatcc
    84481 gcgagcacaa agaagaattt atggaaaaac gggtagtgac ctactgtact ggtggcattc
    84541 gttgcgagaa gttttctggt tggcttgtcc gtgaaggatt caaagatgtc ggacagctgc
    84601 atggcggtat tgctacttat ggtaaggatc ctgaagtcca aggagaatta tgggacggca
    84661 agatgtatgt gtttgatgaa cggatcgctg tcgatatcaa ccaagtagat aaacaaatcg
    84721 tcggtcgcga ctggtttgac ggaacaccat gtgaacgata tatcaattgt agcaatcctg
    84781 agtgcaatcg tcagattctt gcttccattg aaaatgaaga aaaacattta ggagcatgta
    84841 gcatagagtg tgcgaaacat ccgcgcaatc gctatgttca agaccatgga atctctccag
    84901 aagaagcaga actgcggttt acagctttaa ttaaatagaa aacaatagtt ttgattgtcg
    84961 accatttcca atatgcgcta tactagcgtt gaggatggtg atccacatga aatcattgat
    85021 ttatgctatt ggggatgtcc atggcaaaca tgagcttttt gaaaaactta ttcactatta
    85081 tgatccaaag atccatcagc ttgttttatt aggggatctc aatgatcgag gtcctcgtac
    85141 aaaagaatgt tttttattag gaaagcaatt agtcgaagaa acaggagcca tttacctccg
    85201 gggaaaccat gaagaatatt ttttgcaatt tctgcaatca ccggaggact ggtatgcgcc
    85261 ttatatgaga aatggtggaa aagaaacgtt tgaaagcctg attcacccag gggcatcgga
    85321 ggaatactcc ccaactgaaa tctcgatgat gatccaatcg agatattcag aactgattaa
    85381 atttttagtc cagcgcccct tgtattatga atgggaaaat tatctttttg tccatgccgg
    85441 tgtggatttg acaaagaaag actggcgtaa gacaagtcta agagacttta tttggataag
    85501 ggaaccattt catgaaggga aaaacaatac aggtaagaca attgtatttg gacatacgat
    85561 tacaccaatg ctccatggag atatgcagac aacagacctc tggattcagg atcataaaat
    85621 cggaatggac ggtggggctg tattcggagg gtcattgcat ggggttgttt ttgacaagga
    85681 aggtattctt caagatattg aatatcagaa caacgaagga ccttggcatg gaatctgact
    85741 aatggaaaga atgaaggagc tgtgactttg ttgcggctct ttttattttt atagagaata
    85801 ttgtttcttt cagtagttta acccaggata aaaagtatga tagaataaag aagcaatttt
    85861 tagcagaaaa gaggcatttt aatgacggaa aatttgaacc agacgatcat tcagcttgta
    85921 caaaaagaac tttcagacta tcgtcctaag cagttgacta ctgtacttaa tttgttaaat
    85981 gaaggaaaca ctgttccttt tatcgcgcgt taccgaaaag aaatgactgg aagtcttgat
    86041 gaagtccaga ttcgtgaaat cgaagagcgg tatgcctatc ttgaaaactt agaaaaacgt
    86101 aaaactgaag tgattcggct aatcgatgaa caagggaaac tgactcccga gttagaagca
    86161 gaaatcaccc aagccgtgaa aatgcaacaa gtggaagatt tgtatcgtcc atacaaacaa
    86221 aaacgacgga caaaagcaac gattgctaaa gaaaaaggac ttgaaccact cgcgaagtgg
    86281 ctgatgcagc ttacagatgg agaggtgcaa tctgaagcag aaaaatatat tgacaaagaa
    86341 aaagaagtct cttctgccga agaagcgttg cacggtgctc atgagattat tgctgaacaa
    86401 gtcagcgata atgcaaaatt tcggacttgg attcggtctt atacgtataa caaaggtatg
    86461 tatgtcagca atgtcaagga tgagcaagca gatgaaaaag gcgtatatga aatgtattat
    86521 gactttgcag aacccgtcca taaaatggtt tctcatcgaa tattagctac taatcgtgga
    86581 gaaaaagaag atattctgaa agtattcttt caagtagatg aagcagcaat tttagcttac
    86641 ttggatcgcc aaatagtcaa aaatcctgcc tctccttctt cttcattcgt cagagaagcc
    86701 tatcaggata gttataaacg ctttattcag ccagcaatcg agcgagaact gcgcaatgaa
    86761 ttgactgaaa aggcagatga gcaagcaatc gctattttcg gcgaaaacct ccgtaatctt
    86821 ttgttgcaac cgccactaaa aggtaaagtt gtcctgggat ttgatccggc ttatcgtact
    86881 ggatgtaaac tagcagtcgt agatgcgaca gggaaagttt tagcaatcga agttatttat
    86941 cctcataaac ctgcagcgca agcaaaaaga gaagctgctg gaccagcttt tatccaatta
    87001 attaataaat atcaagtaga tatggtagct atcggaaatg gtacagccag tcgggaatct
    87061 gaactttttg tggctgagca gttgaaatcc gccgaccata aaacctatta cgctatcgtc
    87121 aacgaagcag gcgcttctgt ttactccgct agcgaaattg cacgaaaaga atttccgcat
    87181 ctacaagttg aagaaagaag tgctgtttct attgcccgtc gcctgcaaga tcctttagct
    87241 gaattagtca aaatcgatcc taaagctgtc ggagtaggcc agtaccaaca tgacgtttct
    87301 caaaaacgtt tggctgaaca attggatttt gttgtggaaa cagcggttaa ccaagtaggt
    87361 gtggatgtga atacagcaag cccgcagctc ttgcagcata tatcaggatt gaataaaaca
    87421 actgcccaaa acattgtcat ttatcgtgaa gaaaacggag aattcaccgc acgaactcaa
    87481 ttgaaaaaag tgccacgctt aggaccaaaa gcatatgaac aagcaatcgg attcttgcga
    87541 gtccctggtg ggaaaaacat tttggacaac acaggtatcc atcccgaaag ttattctatt
    87601 gcaaaagaaa tattaacttc agtccaactt tcagaaaaag agcttggtac agaagaagca
    87661 gtagaaaaat tgacacgatt atccgtagag aaactggctg aaagcctatc agtcggagaa
    87721 gaaacgttag ccgatatttt agctgggctg acacaacctg gacgagatat gcgtgatgag
    87781 atgccagcgc ccttattacg tacagatgta ttgagtatgg aagacttgaa accagggatg
    87841 gaattgactg gaacggtccg taatgtcatt gacttcggcg catttgttga tatcggtgtg
    87901 aagcaagacg gacttgtcca tatatcgaaa ctcagcaaaa aattcgtcaa gcatcctaca
    87961 gatgtcgtgt cagtaggaga tatcgtaaca gtttggatag aacaagtcga tacgaaaaaa
    88021 ggcagaatca gtttgacgat gctttcccct tatgaagagt aacaatgagg agtaacaaaa
    88081 attgacacaa gaagaactgc agcagttagt agaagagata tcattaaaaa gcttccagct
    88141 tcctttcaag caccaagcaa tgttcaatcc acgcttacgt acaactggcg ggcgctatca
    88201 tttaaacgat catcatttag attttaatgc gaagctattt gaagagtcag acgaagagac
    88261 gattgcagga atcatcaaac atgaactttg ccattatcat cttcatctag ctggaagagg
    88321 gtatcagcat aaagatatgg atttcaagta tcttttgaaa aaaacaggag ggttgcgcta
    88381 tgcaccagcc ttgacgaaga aacagataaa gatggagtat tatcgatgcc aagattgttc
    88441 tgccacgatc tgccgcaaga gaagaatcga tgtacgcaaa tatcgttgtg gaagatgtca
    88501 tggagaaatc aaatggattg agacaaaaag agaagacttt aatgaagcac aaaaaatcag
    88561 atgaaaggat agaaggcctt tggaaaaaag tgagcagagg aatctaaaag actggaatca
    88621 gctattaaat tttttagccg cacctgaaga gggaaatttt gaggaactga cggcacctac
    88681 acttatttta gctggaaact gtcttcctat cttagctgat aaagcagcac aaatgtattt
    88741 aaatggccaa gtagatcaat tgtttttggt cggaggggtg gggcatgcga ctagaatcct
    88801 ttatgagaat tttgaaaagc aaggatttca ttttgaagat ggcatgagtg aaagtgaaat
    88861 ctgtcgtcag tatctaaaag aagtctatga tttgcccgat aaggcttttc tgatagaatc
    88921 gaagtctaca aattctggag agaacgccac tttttcactg gagattcttc attccttagg
    88981 agctgtacca gaaaaaatct tattgatgaa tgatccgaca ctgcaacgca ggactcgagc
    89041 aacttttgag aaagtatggc aaaatgaaca aactattttc gcgaatgctg tgccatttgt
    89101 gccggaaatc cttcactttt ctgaagagat catctttaca gcaaaagaac tgaatcatca
    89161 atggcctaag gaatatttcc atgcgctagt attaggagaa atggaacgtc tccatgacga
    89221 tgagaacggc tatggtccga aaggaaaaga ttttattcct cacattgata tatcagagga
    89281 agtttggtct tcttacacca gaattaaaga gtcaataaaa acggattttt ctcgtacttg
    89341 accattttag caagcgaatc ctcctttcca aataaatttg gttgaaattt tcaatcgttt
    89401 ataagataat gaggagcgtg ttgaatgtaa tagctaaaag aatggacgct agataagaga
    89461 agaggattca aatggaaaat caaaataaat tgtatcattt tgttggtatc aaaggttcag
    89521 gaatgagctc cttggctctt gttttacatg aacaaggtct aaatgtccaa ggatcagaca
    89581 ttgaaaaata tttctttaca caaagagatt tagaaaaagc aaatatcacg attctgccat
    89641 ttaacgcaga taatgtaaaa ccagggatga cgatcattgc aggaaatgca tttcctgatt
    89701 cacatgaaga aatccagcga gcaaaagaat taggtttgga agtgattcgc tatcacgact
    89761 ttattggtca ttttatccaa aattatacga gtatcgctgt aacaggctct cacggaaaaa
    89821 caagtacgac tggactgctc tctcatgtat tgagtggtgt ccgtccaaca agttatctaa
    89881 ttggagatgg aacaggacat ggtgatccgc aagcggaatt cttttcattt gaggcctgtg
    89941 aatatcgccg tcatttcctt gcctattcac cagactatgc aatcatgacc aatatcgatt
    90001 ttgatcatcc ggattattat acaagtatcg acgatgtatt tacagctttc caaacaatgg
    90061 ctggacaagt gaaaaaagct atttttgcgt atggtgacga tgcttattta cgcaaattaa
    90121 aagcaaatgt gccgatttat tattatggtg tcacagaaaa cgatgatatc caagcacgaa
    90181 acatcgaacg cacaacttct ggttctgctt ttgatgtcta tcatggggat gaatttgttg
    90241 ggcacttcac tgtaccagca ttcggtaaac acaatatctt aaatgcttta ggtgttattg
    90301 cagtagctta tttcgaaaaa ctggatctga aagaagttgc agaagaaatg ctgactttcc
    90361 caggagtaaa acgtcgattc agtgaaaaga tcgtagcaga catgaccgta gtcgatgatt
    90421 atgcgcatca ccctgcagag atcaaagcaa cgattgatgg cgcacgccaa aaatatccag
    90481 ataaagaaat aattgcagtt ttccaacccc atacgtttac gcgtacgatt gcattgatgg
    90541 atgaatttgc agaagcactt gatttagctg ataaagttta cttatgcgat atttttggtt
    90601 cggctcggga agaacaagga aacgttaaga ttgaagacct tggagcgaaa atcaaaaaag
    90661 gtggagaagt catcaaagaa aataatgtat ctccattgtt agattatcat gatgcagtag
    90721 taatcttcat gggtgctgga gatgttcaaa aatttgaaca agcgtatgaa aaacttttaa
    90781 gcagcacgac gaaaaatgta ctgtaataca attactgcag tgtccttata aaaatagaaa
    90841 gagggggagt ggcaaaaatc ttgccaagcc cccttttttg caacttatga taaacggtgt
    90901 tcaaaagctt gcatctgcgg taataagccc aatcaaatca aaaacatgag aagctgtttt
    90961 ttcttggctg ttcttattat ttgatgcagg acagcttttt cacgacctct gtaaactata
    91021 ggaatctgtt aaatcaaggg agcagaactt gccttttacg ctacttttgg tacaataatg
    91081 tatgattaaa aataaactgg agggtttcta tcttgaacat aggtaagccg agaaaactag
    91141 gaaaaacaat aaatgaaatc gaggaaggcg actcgttatc cttaacagaa tctattgaag
    91201 ataaccagct tttgctgtat ttgggattga cgaatgatgc aaatcctttg tatatccaac
    91261 atgactttgc acaaaaaaca gaatatcaac agccaatcgt tccatcagtg atgctgatgg
    91321 ggatcatcac aagtgcaatt tcaaagcatc ttcctggacc aggatcacat gttgtcaatt
    91381 tttctgtcaa ttttattgaa ccggttttcc attatgaaac actgactttt cagtttgagg
    91441 tcataaaagt agataagatg aaggatgtgg tgacgatttc tgtagaagcg gagaatacga
    91501 acgaagatcg tgtgttggat gctgtcgtta tggtccaacc gccacaagtt cctgaattgg
    91561 aaatagcaat cgaaagtgaa ggtgaattga atgaataatc caagtacatc aatgtcaggt
    91621 atcaatcgtt tttatgcaaa agtttatgga tttttaggta tgggacttgc ccttagtgca
    91681 gtcacttctt acttggctct ttgggtattt cctttgcaat tagcaacttt tatcaataat
    91741 tttccactag gctttgtggg tctgtggatc gttgaactga ttttagtaat ggttctaggc
    91801 gtaaaagcac aaaagaatcc gagcttagca atcggtggat ttattgccta ctctcttttg
    91861 aatggcataa cattagcggt tactcttgca tactacgata ttggaacagt aacgcaagct
    91921 ttcttgacag ctactttgat gttcatgggg atgtctgttg tcggagtaac aacaaaaaga
    91981 gatcttagcg gggttggaag agcaggacag gcagctttaa tcggagtgat cattgcacta
    92041 gtcttgaaca tcttcatttt gagaagtagc gtagttgagc tgttcatctc cctactgtta
    92101 gtcgtcatct ttgcagggat tacagcttat gataaccaaa gcatccgtaa tatctatgtc
    92161 cagacaaacg gaacagcaca tagtggaatt gctgtattca tggcattgaa gctgtatttg
    92221 gactttatca acttgttctt ggcgtttctg agaatctttg gacggaacaa ctaaaaaaga
    92281 aaaaaattca tcagttgggg ctataccgaa ttatttggta tggtcccgct ttttcttttc
    92341 gcattccgca ggattctttg ttagaatgaa taagaacttt acagtaagga gttaagaaga
    92401 tgacaaaaaa taaattatta ctcgttgatg ggaacagtgt ggcttttcgc gcattctttg
    92461 cgcttcacaa ttctcttgaa cgtttcaaaa atagaaacgg tcttcataca aatgcaatct
    92521 atgcattcaa tacgatgttt gaaaatgtta tgcaaaaaga gcaaccgact catgttctgg
    92581 ttgcgttcga tgcaggaaaa acgacttttc ggacagaaat gtatgcggag tacaaaggtg
    92641 gaagatccaa aacacctggc gaattcaaag aacaaatgcc atatatacgt gatttgttga
    92701 caggactagg ggtacagtat tacgaattgc ccaattatga agcagatgat attatcggta
    92761 ctttggcaga aaaagtagac aaagatcaat ttgatgtcgt cgtgctttca ggtgatcggg
    92821 atttgactca attagctagt aaagaagtaa aagtggatat cactgtaaaa ggtgtcagcg
    92881 atatcgaaag ctatacacca gaacatgtag cggaaaaata tgatgggttg acgccaaaac
    92941 agattattga catgaaggga ctagccggag atgcctctga caatatcccc ggtgtgacta
    93001 aaatcggtga aaaaacagct atcaagctat tgaaacaata tggctcagtc gaaggaattt
    93061 atgaacatat cgatgaaatg aagcaaagca aaatgaaaga aaatctgata aatgacaaag
    93121 agcaggcttt cttatcgaaa aaattggcga ccatcaatac ttcagcgcct gtggatgtct
    93181 ctatcgactc attgaagtat gaagggaaaa atctcgataa actcgttccg ttttataaag
    93241 aaatggattt caatcagttc ctttctaaat tgaatatcgt agaagaagaa gtaaaaatgg
    93301 atgatatttt gtttgaagtc gttcatgaat tcaaagagga aatgtttact acggatatgg
    93361 cgttgtacgt ggaaatgatg ggcgacaatt atcatacaga ggaaatcgtc ggagtagcgt
    93421 gggggacaga aaagaaaatt tatgtcacga acgatctatc tgtattcgaa aaccgagcgt
    93481 tccattcttg gatcactgat ccgactcggc tgaaaaaagt atatgatgcg aaacgaactt
    93541 atgttgcttt aaaccgctat actggaaaaa cgaaaggaat cgattttgat gtcttgctag
    93601 gtgcatacct tctcgatacg aatgaaaaaa gcacagatat cgaaggaata gctgctcatt
    93661 acggatacaa cgatatccaa tcagatgaag ctgtatacgg caaaggggcg aaaaaaggtt
    93721 taccagaaga agaggaactc ttttttgctc atttagcgcg aaaagtagct gcaatcaatg
    93781 cgttgacacc aaaattgagc caagaactgg cagaaaaaaa ccaagaagat cttttccgga
    93841 aaatggaact gccattgtct atcattttgg cagagatgga aataagcggg atcaaagtag
    93901 atgcgactcg cttacaagaa atgaagggcg agttctctgc tcgcttaaga gagatcgaac
    93961 aaaaaatcta cgaagaagcc ggcgaagaat tcaatttgaa ttcaccaaag caattaggag
    94021 tgatcttatt tgaaaaaatg ggattacccg ttatcaaaaa aacaaaaact ggatattcca
    94081 cagccgtaga tgtactggaa cagcttcgtg aacaagcacc aatcgtagag gatatcctca
    94141 cctatcgtca aattgcaaaa attcaatcta cctatgtcga gggactgctg aaagtgatcc
    94201 aatcagatgg aaaagtgcat acccgttatg tccagacatt gacccaaaca ggacgattaa
    94261 gctctgttga tcctaatttg cagaacatcc cgattcgatt agatgaaggg agaaagatac
    94321 gtcaggcatt tgttcctaga gaaaaagact ggctgattta ttcttctgac tattcacaaa
    94381 tcgagttacg cgtacttgcg catatctcaa atgatgaaca tttgaaagct gccttcctag
    94441 aaggtcaaga tatccatgcc agcacagcga tgcgtgtatt tggaatcgaa aaggcagagg
    94501 atgtcacacc aaatatgcgt aggcaggcaa aagcagtgaa ttttggtatc gtgtatggca
    94561 tctctgatta cggactttca caaaatctag gaatcagccg aaaagcagca cagcaataca
    94621 tcgatacgta ctttgaaaaa tacccaggtg tcaaagaata tatggaaaga atcgtacgcg
    94681 aagcaaaaga ccaaggctac gtcgaaacgc tgtatcaccg ccgtcgatat ctgccagaca
    94741 tcaattcaag aaattacaat atccgttctt ttgcggaacg aacagctatc aatacaccaa
    94801 tccaaggaag tgctgctgat attttgaaaa tcgcgatgat cgaattggat aaacgattaa
    94861 aagaaacagg tcttcaagct acgatgctgt tacaagttca tgatgaactt gtcttcgagg
    94921 taccggaaaa agaacttgaa tcattagata aattggtgaa ggaagttatg gaacaagctg
    94981 tttctcttca tgtaccactg atcactgaca gcagttgggg aaaaacgtgg tacgaagcaa
    95041 aataaacatt gtttaagaca gagcaatata atgaaggtga aaagaaaaaa tgccagaatt
    95101 accagaagta gaaaccgttc gtaaaggatt ggaacggtta gtagtaggaa aaacgattca
    95161 aaaagttcag gtcttgtggc ccaagatcat cgagcaacca gagacaccca tctttgaggc
    95221 ttctcttgtt ggcgaaacca tccaatccat tggacgtaga gggaagtttt taatttttca
    95281 tctcgatcat tgtgaactga tctcacactt gcgaatggaa ggaaaatatc aatttacgaa
    95341 agaaaataca ccaatcgata aacatacgca tgtacttttc ttttttgagg atggaagcca
    95401 acttcgctac aacgatgtac gaaaatttgg tcgtatgaca atcgtcgaaa aaggagcaag
    95461 tgcaacttac cgaggaatca tgaaattggg acctgagcct ttgccagata gcttcttatt
    95521 agcagatttc gcaaatggac tgaaaaagtc ccacaaagca atcaagccat tgctgctaga
    95581 ccaacgtcta gtgactggtc taggtaatat ttatgtagat gaagcattat gggaagcgaa
    95641 gatccatcca gaacaaccgg cgaacacact gcgccccaaa gaggtagaac aattacgtct
    95701 atcgatcatt gatgttttgg atcgtgcaat agaagccgga ggtaccacga ttcgcagtta
    95761 tttgaatgca ttaggagaat caggcggatt tcaagtagca ctgcatgtct atcagcaaac
    95821 tggcaaacca tgtatacgtt gtggaacgcc gatcgtgaaa acaaaagtgg cacaaagggg
    95881 gacgcactat tgtccgaatt gtcaaaggct aaaaatcgca aagtaggatt tgttttaggt
    95941 ttgacgggtg gaatagctac cggtaaaagt acggcagcca aagtctttca gtctcatgga
    96001 tttcctttga tcgatggcga tgtgattgcc agagaaactg ttgagcctgg aacgcttgct
    96061 ctgaggaaaa ttgtatcagt gtttggccaa gaggtattgc agcctgatgg gcaactagat
    96121 cgagggaaac ttgggatgat tgtttttcca tcaaaggaaa aacggcaaaa acttgatcag
    96181 ctattagatc cgtttatccg aaaagcgata aaagatcaaa tcgcctctct aagttccaat
    96241 catcctttag taattgtaga tatcccattg ctctacgaag gtcattatga ccactatatg
    96301 gatgcagtag ctgtcgttta tactacgcca gaaactcagc ttcagcgttt gatgaaacgt
    96361 aatcaactga cgctagaaca agcgcagcag agaattagta gtcaacttcc aatcgaagaa
    96421 aaaaagcagc gtgcggatat tttgttcgac aacaatggaa caaaagaaaa acttgtggga
    96481 caaatcgaaa attggctttt tgctaatcgc tttctttgag tatctgtgtc aattttgcta
    96541 tcctgagtat atagaaagtg agggatagcg atgggatcat taaaaaatca aaaggataaa
    96601 gtcgtaggta acgcaaaaga aaaaatcggc gagatgctca ataaagaaaa tctaatcgat
    96661 gaaggtcgta cgcaatcaga aacagcagaa gaaaaagaaa agcagtacca agcaagaatc
    96721 gatgaaatag aagaccagca ggcagaagaa gaagcgaagc ggcaagattt gactcaagaa
    96781 cgaaaaaatc cgacacctga tcgtagtaaa acaccgctcc cagaaagtga ccacattccc
    96841 gatgagcaaa aaatcgagga acagcgaatg aaacactata caggaatcga agagaagtta
    96901 taaaatagta aaaaatcgag atgtgacttg aagcagatga gaaggtgttc atctgcttca
    96961 agttttttgt gctagatgac taaatcagct tttgtgtaag ttatttctca gtaatcaagg
    97021 tgatcagtat tggaaaattg aaatttagta aggacagggg acacaattac gcatgaaagt
    97081 agaaaaaatt gttcttatat ccgtgttttt tgggtaggac agtcgcgttt gtgatttgtt
    97141 tcgtgttata atacaactag ttgtaaagaa agacacatcc tttatataaa atgcgatgca
    97201 agaggtgaga caaaatgaga tgtcccagat gtcaacataa taattcacga gtaatcgaca
    97261 gccgtcaagc agatgatgga cgtgcgatcc gtcgccgtcg cgaatgtgag aattgttcgt
    97321 tccgattcac tacatttgaa cgaattgaag ctgctccgtt attagtaatc aagaaaaacg
    97381 gggaacgcga agagttcaat cgcgataaaa ttttaagagg actcatccgt tcagcagaaa
    97441 aaagacctgt agctatggaa caaatggaac aaatcgtcaa ccatgtcgaa aaccgcgtac
    97501 gcagtctcgg agaaaacgaa gtatcgacga atctaattgg tgaatatgtg atggaagatt
    97561 tagttgattt agacgaaatc gcttatattc gttttgcaag tgtttatcgc cagttcaaag
    97621 atatgtctgt tttcttaaaa gagttgcaag atatcgtgga taaagcaaaa gaaaataata
    97681 aagagaataa taaagaaaac aattaagaaa caaaaaggag gcatttcctt ggaaaatgct
    97741 tggaaagagc ttcaaccaaa aaatatttac caaggaagaa aagcggctcc gctgagagaa
    97801 gacagcaaga acgccttgct gtatctttat cagccactaa tcggcggaga agccttgagt
    97861 atctatttca ccctgttatc agaaatctct caagaatctg gcaaaggtcc tgaagggatg
    97921 catgcagatc tgcttagtag tctaggctgc ggtatcccgc aattttatga tgcaagaaaa
    97981 aagctagaag gaatcggctt actggatgtt tattttaaag aagatagtga tctgggacct
    98041 cgttttattt atgagctgtt agaaccactc attcctaaag catttttcaa agactctgtt
    98101 ctggcttttc ttcttctgga aaaagttggt gaacgacgat tccagaagct tacccaattc
    98161 tttgaaccta aaaggcatac agcagaggga tatcaaaaaa tcacaaaaaa atttttagat
    98221 gtttatcgct tcaatgaagc agtgtacgct gctaatcaag aaaacatcga atcgctgaat
    98281 aaatcttttg aagaatctga atcagtagca ttacttgaag cagattcgac attcgattgg
    98341 cagtttttga atgattggct ggcaaaaaaa catattgcaa gtcttgatga tccaatcatc
    98401 aaacaagtcg acatgtatca tcagctttac ggtttcgatg aattgacact tggcgaactg
    98461 atcagtcaat cttatgactt tagtgaacaa aaagtatctg aaaaagaact gcaaaaagcc
    98521 attttggcaa tgcagagacg tccggtgaag caaacaaaag aaacagttgc ggtttctgaa
    98581 aacagtaatg aaccctcgtc aaacgaacat ttgacaaacg cagcgcttca gctgataaaa
    98641 gaagcacaga gcattccacc gatgaaatat ctggaagcaa tcaagtcaga aaaaggcgga
    98701 tatgtttcta aacaagaaaa ctggttactg caagatcttg tttctcaatc aggtctttcc
    98761 agtagcgtga tcaatgtttt gctgaattat gtactagtga tcaagaataa tgctagctta
    98821 aatgccagtt atgtaaatac agtggcaaat gaatgggcac aaaagaaaat cgccacagca
    98881 gaagaagcga tccagcacat tcggcaaata agtaatcaag caaaacaatc aaaacaaaag
    98941 aagcaaacaa attatcaaac tggcaagcga aatgttcgac gggaaaagct tcctgattgg
    99001 gtcaaccagc caaaagatga aaaacagatc agcagcgaga aaaaagcaga gattgaccgc
    99061 cgttttaaag aatacctggc aaaaaaggag ggtgatatct agtggaagac gtaggcaaag
    99121 aattgaagag gatcatcagc aaacgcaatt atcaagaaaa gtatgctgaa atgatcgctg
    99181 aagtcttaaa tgatgcggat gttcaagctt ttctaagcgc acaccaagaa gaattgacac
    99241 aagcggatat tgaaaaaagt tacgctaaat tgtatgaatt cgtgcaagaa aagcgtaaat
    99301 atcaaaaaaa cgatccaacg atgatcgctc ccggctatga gccgaagctg acgttgaatt
    99361 ttcattatgt ggatgtcact tatgtaccaa cggaagcatt gttagcacgg caaagacaag
    99421 aagaaatcag aagccgagta aaagcaatgg atatgccaaa agatattcaa tcggcttctt
    99481 ttgaaaactt tgaacgtaca gatggacgag gatatgcgag cttagaagcc ctagattttg
    99541 tagagaaata tactgctgat ccaaaagggt atcataaagg actgtatcta gtaggaagtt
    99601 ttggtattgg aaaaacatat ttattaggag caacagcgag agaattagcg attgctggat
    99661 tcaccacgac gctagtccat tttccgacat ttgccgttga gatgaaacag gcaatcggaa
    99721 aagatcaagt ggcagaaaag ttagatgcag tgaaacgttc acctattttg atgatcgatg
    99781 acattggtgc agatgcgatg agtagttgga tacgagacga agtattcggt gttatccttc
    99841 aatatcggat gcaggaacag ttaccaacct tcttctcatc gaattttaca atggatgaat
    99901 tggaacagca tttgtccgtc actcaacgtg gagatgaaga gccgctaaaa gcgaaacgga
    99961 taatggaacg catacgttat ttgacaaaag aaatcgaaat gactggtcgc aaccgacgca
   100021 atggttgaac agttgacaag tccaactgac atttgaccta gtagtaaaag caggtcagcg
   100081 aatcattata tggacagaaa tgaccggaga caatcaccgc aacaagtaat gcagaaactt
   100141 aaacggagga ttttttatga gggctatttt ttctagtata tctgtagttg gtctattttt
   100201 tcttttagca ggctgcggta gcgcagatag caacacaggt tctgttgatt cgattgatgc
   100261 gggtcaaaca gtaaaaacaa gcagtacgaa aacggagaga caaagcatat ttgtcggaac
   100321 gattgaaagt atcactcaag tagaagcaga agaagaaacg cttcagttga aactcgtcga
   100381 tgtcatggcc aaagaagatc cagcgaatat gggaacgtca ttcagcaatg acggagtgat
   100441 cttaaatgct actgctgaaa aaataagcgt ggacataaat gaattgaaaa tcggtgacaa
   100501 agtccaattt acattagttg agatgccgat catgacgatg tctatcccac cgcaagttcc
   100561 tggagattcg attattgatg tttcattgaa caaataacca ataacatttt ttcaaaaata
   100621 agaaaatgtt gttttataga acgaaaaggt atatttttta aggagatatg ggaatttttt
   100681 tattcctagt ctccttattt tatttggaaa atgctataat tatctagaag gagtgaagct
   100741 atgaaccatt tagatttatc tcagcgctta gccgcagtgg gtgactttgt ccctgaatcc
   100801 gctcgtttag cagacattgg atcagatcac gcttacttac ccgttgcatt gatattaaga
   100861 ggaaaaatcg aatatgcagt tgctggtgaa gtcgttcaag gtccttatga atcagcacgt
   100921 aagcaagtca aaaaagatgg attagaagac cgtatcaccg tacgccttgc aaatggtcta
   100981 gatgctatcc agccacaaga tggtattaca gctgtcacga ttgcgggtat gggaggttca
   101041 ctgatccgag atatcctaga agctggcaag ttgaatcaac gcttaagcgg acaagaacgt
   101101 ttgatccttc agccaaacat cggagagcgt acattacgat cttggctgca agaaaatcag
   101161 tataaaatcc aatcagaaaa aatacttgaa gaaaataaaa aaatatatga aatcattgta
   101221 gccgaaaaag aacaaaaacc agttacttac aatgaaatgg agctgatgtt cgggccattt
   101281 ctgcttgaag agaaaagtga gatcttcaaa tcaaaatgga caagagagct tcaacaaaga
   101341 aaagctatcc gtgaacagct caaacaagca gctaatcaag aggcacgtca aaaacaaatc
   101401 gagcaggaaa tccaaatgat tgaggaggtt ttaaagccat gacaatcagc ggacgcactt
   101461 tcatccaaaa gtttgaaagc tattgtcctc agtggctagc tgaagaagga gacccagtcg
   101521 gactgcatat cggaacattg gataagccaa tccaacgagt aatgatgaca ttagacgttc
   101581 gtccagaagt ggtcgaagaa gcaatcaaga aaaaaatcga tttgctgatt gctaaacatc
   101641 ctccaatctt tcgtcctgta aaacggctgg ttactgatca gccacaagaa aaaatgtatg
   101701 cagatttgtt gaaacatgat attgccgtct atgcagcaca cacgaacatg gatattattt
   101761 gggatggact gaatgactgg ttttgtgaac ttctcggtat tgaagtggag agttatttag
   101821 taaagactca tgagattcat tataaaaaac tggccgttta tgtacctgtg gatcatgcac
   101881 agaaaatgcg tgaagtatta gctgcagctg gtgctggtac acaaggtgat tataccggta
   101941 caagttttac ctcgattgga cacggtcgat ttactccgga agctggtgca caaccagcga
   102001 ttgggaaagt agggaagaca gaacaagttc aagaggcaaa agttgaggtt attttgccgg
   102061 aaactatcga aaaacaagtg attcaagcca tgcggtcagc tcatccttat gaggaacctg
   102121 cttatgatct tttcgctata gatgaaccag tcgaaatgtt tgggttagga cgagtaggtg
   102181 aattgccgca agaaatttct atagaggcat ttgtagaaca agtaaaagaa gcgttccaac
   102241 tagatggact gagaatagtc cagccaaaaa atgcaaaatc ctctgtgaag cgcattgcga
   102301 tttgcggtgg aagcggtgaa aaattttatc cgcaagcaat cgcccaaaga gcagatgtct
   102361 atatcacggg agatatctat tatcatacgg ctcatgatat gcaaagcgct gggttgattg
   102421 caattgatcc cggtcattat attgaatcgt tatgcaagca acgatttatc gagaaatttg
   102481 aatcttggaa acaagaagaa aattgggata ttaacttttt cgtttctgaa acaaatacaa
   102541 acccatttca atttaattaa aaggagagaa tactatgtac gaaaatcttt tgccacgttt
   102601 tttacgatat gtcaaaacag aaacacgctc cgatgcaaca agcaaaacga ctccatcgac
   102661 tcagacacaa gttgcttttg cgcaagaatt gaaaaaagaa ttagaagagt taggcttagc
   102721 tgatgttttc tataatgaag ataacggatt cgttatggga acattaccaa gcaatgttga
   102781 tcatgaagtg cgttccattg gatttatttc ccacatggat acagcagatt ttaatgcagt
   102841 aggagtcaat ccacaaatca ttgaaaacta tgatggcgag tctacgatcg tactagatgc
   102901 ggaagaacgc tatacattga atacaaaaga ttttccaaat ttgaaaaatt atgaaggcca
   102961 cacattgatc acaacagatg gcagtacatt actgggagca gatgacaaat cagggattgc
   103021 ggaaatcatg actgcaatgg agatcttgat caataatcct tctatccctc acggtaccat
   103081 caaagtggct tttggtcctg atgaagaaat cggtgtcgga gcggataaat tcgatgttga
   103141 gcaattcgat gtagattttg cttatacagt cgatggtggt cctttaggtg agttgcagta
   103201 tgaaacgttt agtgctgctc aagcaaatat tacgatccaa gggaaaaatg tacatccagg
   103261 gacggcaaaa gatacaatga tcaatgctct acagttagca atcgattttc ataatcagct
   103321 tccagccgat gaagttccag aaaaaacgga aggatacgaa ggtttcttcc atttgatggc
   103381 attggatggg aatcctgaag aagcttctat gagttatatc attcgcgatc atgatcgtga
   103441 aaaattcgaa gcacgcaaag cacaaatcac acatatccaa gaagagttga atcagcgatt
   103501 tgatcaagaa cgaatcaaag tagatatgta tgatcaatat tacaacatga aagaaatcat
   103561 tgaaaaagat atgagcatcg tggaattagc gaaacaagca atgatcgagc ttggtatctc
   103621 tccagtgatc gaaccagttc gtggaggaac agatggttca aaaatctctt acatgggtat
   103681 tccgacgcca aatctatttg ccggaggaga aaatatgcac ggacgattcg aatttgtttc
   103741 tttacaagta atggaaaaag caacagacgt gattgtaaaa attgcagaat tgaacacaaa
   103801 gagttaaaat cgaaaaagaa cagcttatga caaggcgttc gtcccaatga tggaatgtca
   103861 tctgtctaag gctaaagggc aggacaatgg aattggttgt cccgccctct tttttgaaga
   103921 gtaaatagga ggtaaagaat ggaaaagctg ctagtttttt ataacaaaag ttcagggaaa
   103981 gatgagggtg agaaattagc acgctggttt caagattatg taaaagaaaa gcagccagaa
   104041 ttatatgtag aactagctga aactggccca tcgattgatg atgcggaatt agtcaagaaa
   104101 gcagaagcca tagaagctga tactttagta attataggtg gagacggcac gattcatcat
   104161 atcgtccaag cgttccaaaa aacattatct caataccatg tcggattact tccaggtgga
   104221 actgtcaaca accttgcacg tgttcttgag attcccctca aaaaagaaga agctgctgac
   104281 gtgatcctgg aaggcaatgt acggaaaata gactatggtc aagtcaatga tgaagtcatt
   104341 atctctacac tgacaatcgg aatcttagca gatacagcag tttggatcag tcaagaagaa
   104401 aaacagcgtt acggctcgtg gatctttatc aagaagtttt ttaagttatt gctgaagaag
   104461 aaaaaatatc atttggattt agaaacagac gaggagcatt ggaaaggaaa atcccaactg
   104521 ttgacagtca cgatgtctaa ctccgtgggc ggttttacaa attttgacga ctcagcgacc
   104581 ctagatgatg gaaaatttca tgtaacgatc gtaccttcct tagatatttt ccgatttatt
   104641 ttttatttgc ctagaatcat gagaggcaaa ttctatacga tccccggcat tacttatttc
   104701 acagccaaac gcatgaaaat ccaagcgcag gaaaaatctg tgggcactcg aacggatgga
   104761 gacacgacag atgatcttcc agttgaatta tctgttgtat caaaaggatt aacagtttat
   104821 tcaccaaaga aagaagaaaa agcttagaaa tagtagttaa cagaaagttg gaagaagagt
   104881 aatggatgta ttgttacaca atcctttgtg gttttacctt ttcattgtga atttttactt
   104941 attttgtctg atggggtatg ataaacatca ggctagaact agcggctggc gtgttcctga
   105001 gaataatttg atatttgtca gcctgttagg cggtggtttg ggaggattga tctctcgaaa
   105061 agttttccat cataaaactc ggaaaaagaa gtttactata ggattcgctg caggtgtact
   105121 tgttgatttg attctcattt attttttcca ttaaggagca ggagatgtcg tctaaaggaa
   105181 cgaaaactaa aatcattctg aatctgcttt tgctgattgt gatttttgcc attatttatt
   105241 atttaatcaa tcaatcattt gcagatatct ttgccgaatt gctgtctacc agtgttgttg
   105301 ttttggcagc ggctgttata ttagggacag tttaccaaat cgctgaaggg cgctcaatca
   105361 aagaaattgc tgaacctttc gcgccgaatt ttacaacgaa agatgggttc ttgacctcta
   105421 gctatattgc gttttaccga atcgtttcgt ttggtacagg aacgctgatc tcggagattt
   105481 atttttataa caaaaaagga atgaagctat cccagggagc aggggtgaca gcactccata
   105541 tgattatgta caaactggca gtgatcactt atgcattagc tggcttggtc atccaattct
   105601 ctctttttta ttctaatggt ccatcaatga tctggtttat catcgctggt atccttttga
   105661 cgatcgtgat tgttgccttt ttattggcga tgtccgtcag cttgaatgtt caaatatttt
   105721 ttgtcagctt atcgaataag ttatttaaaa gtgagcgttt gcggaaatgg gtggatacat
   105781 gcaatcagca gatctactct ttaagggaaa cggtccaaac cattatccaa gacaggactg
   105841 cgctattgcg gatttatcta tggaatatgt taaaacttgt attttggtat gtacttcctt
   105901 atttgattct gattgaaaac catcctaaaa tagattttct actgaccatt tcgtttatca
   105961 gctttgcagt agtgctttca ggggttattc ctacgcctgc tggtattggt tcctttgaat
   106021 ttgtctattt gttattattc agaccagttg ttggaacagt tgacgcagtt tcttcgcttt
   106081 tactgtatcg tttcagttcc tttatcttgc catttctcta tggatttgtg tatgtgattg
   106141 ctgaacggcg aagagtgatc aaagaagaaa taatagaagt aaaaaaagag aataaagaaa
   106201 atcaagggta ggtcttttat aagatctacc ctttttatca aatcaaagaa aaacgttatg
   106261 gataagataa ttttacgttt gtttggctct ttttttatat acatacaatc aattttgttt
   106321 ttttaagatt tatataagtc gagaaaaaaa gtcaaaaaac tatgtgtata ttttgaccaa
   106381 aattgaccaa agcgatataa tagtttttgt aaagatcaaa gaaataagtg atcttacctt
   106441 taattaggag ggtatacctg tatgaatatt gagaaaatga ctacgacgct tcaagaagcg
   106501 attgctgaag ctcaacagat tgcggtcact cgaaaacatc aagatatcga cattgcccat
   106561 gtatggaaaa tctttttaca atctaatcat tttggtcgaa atttctatac ggatgccgga
   106621 ttggatgtcg aatcttttga acatgaaatt gatcgtctac tggatgagta tccagttgtt
   106681 tctggtggaa acgtccaata tggacaaaac ctcagccaga atttctttcg attattgaat
   106741 gaagcagacc aaatacgtga aagcttcggc gacgagtttc tttcaacgga agtcgttatt
   106801 ttagcgttga tgaaattaaa aaattatccg ttaactgttt atttgaataa aaacggatta
   106861 tctgaaaaag aattgcgtaa aaacatcgaa gagatgagag gaggagaaag agtgacctca
   106921 caaaaccaag aagaacaata caaagcatta gaaaaatacg gcgttgattt agtacagcaa
   106981 gtcaaaagcg gaaaaatgga tccaatcatc ggtcgtgatg aagaaatccg tgatgtcatc
   107041 cgtattcttt cacgaaaaac aaaaaataat cctgttctga taggagaacc cggtgtaggg
   107101 aaaacggcaa tcgttgaagg gcttgctcaa cgtatcgtac gcaaagatgt gcctgaaaat
   107161 ttaaaagata aaacgatttt ttcattagat atgggtgcat tgatcgctgg agcaaaattc
   107221 cgtggtgaat ttgaagaaag actaaaagca gtcttgaaag aagtcaaaaa aagtgatgga
   107281 cgtatcctct tgttcatcga tgaaatccac aatatcgtag gggcaggaaa aacagaagga
   107341 agtatggatg ccggaaatct gttgaaacca atgcttgctc gcggtgaatt acatttgatc
   107401 ggtgccacaa ctttagatga gtaccgtcaa tatatggaaa aagacaaagc gttggaacgt
   107461 cgctttcaaa aggtattagt caaagaacca acagtggaag atacgatcag tattctgcgt
   107521 ggattaaaag aacgttttga gatccaccat ggtgttaaca tccatgacaa cgcattagta
   107581 gcagcagcta ctttatctga ccgttatatt actgatcggt tcttaccgga caaagcaatc
   107641 gatttgatcg atgaagccag tgcaacgatt cgggtagaaa tgaactctat gccaacggaa
   107701 ttagatcaag ttactagacg tttgatgcag ttggaaatcg aagaagctgc actgaaaaaa
   107761 gaatcagatg atgcaagtaa aaaacgcttg aaaaatttgc aagaagaatt agctgaactt
   107821 cgtgaagaag caaatgcgat gaagatgcag tgggaaacag aaaaagaaga agtcaattct
   107881 gtttcagcaa aacgtgctga aatcgataaa gcaaaacacg aattagaaga tgcagaaaat
   107941 aactatgatt tagaacgcgc tgctgtattg cgtcatggta cgatacctca attagaaaaa
   108001 gaattgaagg aattagaagc taaagcaaaa gacagcgaga ttaagatggt tcaagagtcc
   108061 gttaccgaaa atgagattgc tcaagtcgtt ggcagattaa cgggtatccc cgtaacgaaa
   108121 cttgtagaag gtgaacgaga aaaactaatt aaattgaatg aaacacttca taaacgagtg
   108181 attggtcaag atgaagcagt tgatgcggtc agtgacgcgg taattcgttc acgggctgga
   108241 ttgcaagacc caaatcgtcc tctaggttct ttccttttcc taggaccaac tggtgtcgga
   108301 aaaaccgaat tagcgaaagc gttagctgag aacttgtttg actcagaaga tcatatggta
   108361 cggatcgata tgagtgaata tatggaaaaa catgctgtgt ctcgtctagt cggagctcct
   108421 ccaggctatg taggttacga agaaggtggt caattaacgg aagctgtcag acgaaatcct
   108481 tatacaatcg tcttgttgga tgaaatcgaa aaagcgcatc ccgatgtctt caatatcttg
   108541 ttacaagtcc tagatgacgg acgcttgact gattcaaaag ggcgagtagt agatttcaaa
   108601 aatacggttc tgatcatgac cagcaatatc ggttcacagt tactgctgga aggcgtgact
   108661 gctgatggaa cgattccaga agcagtggct gaacaagtca acacgctatt acgtggaaac
   108721 ttcaaaccag aattcttgaa ccgaatcgac gacacgattt tatttacacc attgagccta
   108781 gacaacgtga aaggaatcgt tgacaaaatg gtggcgcaat tggcacaacg tctggaacat
   108841 caagaaatcc tgctgacaat cagtgatgaa gccaaaacgt ggattgctga aaatgcttat
   108901 gaaccagcat atggtgcaag accattgaaa cgatttatca ctaaggaagt agaaacacca
   108961 ctagctaaag aaatcgttgc aggacatgtt atgcctaaat caaaagtaac gattacttta
   109021 cttgatggac aacttcattt caaaacagaa gagctagaag aaatcgtcta atcttccatt
   109081 gagactataa aagaaaaaag gggtctgaca caactagtgt cagacctcct tttttcaaat
   109141 aaacagtgtt ccagaagtag tttcttcgaa aaataagccg aaatttcttg aagctgcccg
   109201 cttctgtcac aacctctttt attaggtatt tttcactttt tctaatttat aactttcaaa
   109261 gtaacgctca tcttttttac tttgagacca tgcaaacaat cccattaagg cagcacctat
   109321 cgtatttgtc agcaagtcgc ccatcgtatc catcaatgct gctcgtccga caaacagtgt
   109381 gccatcagaa gcagcaaaac gttgaagatt catgcctaag aactggtcgc ataagaattc
   109441 ccagaactcc caaaacacgc cgcaaagtcc agcaaacgca aagccgaata agaggaacaa
   109501 ccaaggactg gtttttgata tctcagcatc tttcatcaag taaccaatca atccatagcc
   109561 aagagctgtc aggaccattg gactcaccgc atgtaagatt ttgtcccaaa aagagatgat
   109621 actgatcaaa tgcatccctg tgcctaaaaa gacagagatg aacaagaaaa accagtaaaa
   109681 caaaacgatt gctgctggta ttttaagttt gaaaactttc aaaatgactt gtggcatgaa
   109741 aatcaatgca atccctaata aagattccac gataaatgtg attccttgag aagtcgaata
   109801 tttttgtgtg ataaattcat aaaaattata gcagacaacg aatacaccaa aagcaagtaa
   109861 agctatttgc acctttttga tttctttaat cactgaaagt ccctccatct aaaaatcttt
   109921 ttacttctga aagcaactca atttctgaag aaaccacttt tccgttcaat ttgatcaaac
   109981 ctattgtata taagttcagg tagtgaaatt gattttcagc tgtttcttgt agtgcagcga
   110041 tttttttcgg gtgggttccg ccttgctgtc gagtatcagt atataagccg atcactggga
   110101 tgcctttcgc ataagccaca ccgatttccg atgctacccc ggcatcgatc gtcagcccat
   110161 ctagtaaagc aatcattaaa tcactagcca acactttttc tgtgtcagca agagcaatca
   110221 ttcgactgtc tgcataagca gttttgtcat tgattgcttc attttcttga ggaagataga
   110281 tcgaaagctc agaagagagc tctctgattt ctttaactaa ctgttggtta tataacaaat
   110341 cacttttagc aaacattggt gcggcaaaat aaattttcat ctaaacatca tcctttttat
   110401 caaaaataat cgctaaagta agcttccatg tttggaatga tacgctctag tgacttcagt
   110461 gtttcttcat ctgtttctaa ccgttcgatt cttgccaaat aagaagcacg gcgataattc
   110521 atcatcaagc aagtcaatgt ttggatatcc agtcgaacgg attttcctat aggttcattt
   110581 aagacacgga cttgtccctg ttcatcccat gttaaagcga agacaccatt attccaagga
   110641 gcgacaggat cttcaatgat gaaatgaaaa gcatcagctg ttccaacaaa cggaaaccgt
   110701 tgaaggaatt cttttacatc aacgatccga gccataaaat atggttcgat ctgctctttg
   110761 atctgactat cttcaatcaa aaatgctaaa ggctcatttt tataaatatc tccatgtacc
   110821 cagtagatca tggaaaaatg tgcggaaata aagttccata agccatttcg agcttcttga
   110881 tcaagataga acatttcttt tacgtgaaat atttcttcag ctacccagta gaacagaaca
   110941 cctcttggtt catggttgct atcgtagtag acagcggctg tgcgctcatc ttcattttcg
   111001 aaacgccaat actcttccca atgaaaatca ctgcggaata aagcaccatg gttttgttgg
   111061 gcaaatttgt cataaacgga aaatacgtct ggatgatcaa ctggtaatcg ctcaaccata
   111121 ccacttactg ggatttgttt aggtagctgt gtatctctga ttttaaagga aagtttatca
   111181 gacatgattt cccatccttt acgccgatag taggggatac tgtaaggata caaataagag
   111241 atccattgct tattcttgcg catattatcc aatgcaacga taattagatc ttgcatcaat
   111301 ccatggttag catattctgg ataagttcca actccagtca caccacccat tttataacgt
   111361 gcaccgtgaa tattgacttc acaaggatag atagcgattt gagagatcag tttattttcg
   111421 tggaaccatc cgaacacttt cgatacttcc aaaatcggtt ttttggaacg gataaaagct
   111481 cgtttattct caaacccgct ttcctcgatg tctgcttcag tcacttggaa cacataggaa
   111541 agcaattcat tgaattgatc gatatgttct tgagtgaccg gttttaattc aagattttct
   111601 ctaaaatctt tttgatccat tttttcatct ccatttatta gcttgataac agtatagcaa
   111661 aattctacaa aaaaaacttc tatagctgac taattaaaaa ctctttaaat tactgctttt
   111721 cgttggtata atggaaagtg ctgattatat gaaaagagga gacgctatgg atataaagaa
   111781 aatgaaagaa cgccaagagc agattcgtaa tttttcgatc atcgctcata tcgaccatgg
   111841 gaaatctaca ttggccgatc gtattttaga aatgacgcac actgtgacgt ctcgcgaaat
   111901 gcaagatcag ctgttagact cgatggattt ggaacgtgaa cgtggaatca cgattaaatt
   111961 aaatgctgta gaactcaatt acacagcgaa aaatggagaa acatatatct ttcacttgat
   112021 tgacacccca gggcacgtcg acttcaccta tgaagtctct cgaagccttg ctgcctgtga
   112081 gggtgccgtt ttagtggtcg atgcagcaca aggaatcgaa gcacaaacat tggccaatgt
   112141 ttatttagcg ttagacaatg atttggagat cctgccagtc atcaataaaa tcgacctacc
   112201 cgcagcagat cccgaacgtg tccgtcaaga aatcgaagac gtaatcggaa tcgatgctag
   112261 tgaagctgta ttagccagtg ccaaatcagg gattgggatt gaagatattc tcgagcagat
   112321 cgtggaatat gttcctgcac caagtggaga cttagatgca ccgctaaaag ctttgatctt
   112381 tgactctatt tatgatagtt accgcggtgt tgttctaaat gtccggatca cggacggtat
   112441 ggtcaaaccc ggagacaaaa tcaaactgat gagtaatggc aaaacatttg atgttacaga
   112501 ggtcggggtc ttctcgccaa aagcagttgc tagagacttt ttgatggtcg gtgatgtcgg
   112561 ttatatcaca gcaagtatca aaactgtcca agatacacga gtaggggata cagtaacgtt
   112621 agcagacaat cctgccgctg aagctttacc aggttaccga aaaatgaatc cgatggttta
   112681 ttgtggtctt tatccaattg atacttctcg ttacaacgat ttgcgggaag ctttagaaaa
   112741 actgcaattg aatgatgctg ctttacaatt cgaaccagaa acctctcaag cattaggttt
   112801 tgggttccgc tgtggattcc tcggacttct tcatatggat gtcgtccaag aacgcttgga
   112861 acgggaattc aatctagaat tgatcacgac tgcaccatcc gttatttacc atgttaataa
   112921 aacggatggt acgactgcta cagtagataa cccggcagat tttcctgaac ctgtaacgat
   112981 tcaggatgta gaagaaccat tcgtcaaagc acagatcatg gtcccaaatg attttgtcgg
   113041 tgcagtaatg gaactttctc aacgaaaacg gggagaattc attacgatgg attatttaga
   113101 tgattaccga gtaaatgtcg tctataatat tccgctttct gaaattgtct ttgacttttt
   113161 cgacaaattg aaatcaagca cgaaaggcta tgcctcattg gattatgaaa tgtctggtta
   113221 tcaaaaaagc aagttagtga agatggacat cttgctgaat ggagaaaaag tcgatgcatt
   113281 aagctttatc gttcaccgag actttgccta tgaacgtgga aaagcaatcg ttgaaaaact
   113341 gaaaaaacta atcccacgcc aacaatttga ggtgccaatc caagcagcta ttggacaaaa
   113401 aatcgttgcc cgttcagaca tcaaagcctt gcgtaaaaac gtcttggcta aatgttatgg
   113461 tggagatgtt tctcgtaaac gtaaactctt agagaaacaa aaagaaggga agaagcggat
   113521 gaaacaaatc ggctccgtcg aagttccgca agaagccttc atggccgttc tgaaaatgga
   113581 tgagcaagat agtgtgaaat aacttataat caatcgaaaa acctattaaa taaacctttt
   113641 tgagtagatg caacactgag gattatctgt ataaaagatt ttaaactgtc atttggttat
   113701 cacggcgata ctaaaattaa caaaattgga atatttttct tgaagatttt tagctgtcag
   113761 tatatgatga aagcatctca taggaggtgc ttttttcatg gttgataatt caagtggaag
   113821 ggttcttaaa tctctaagaa aagagaaaaa actatctcaa aaaaagttag ctgatttagc
   113881 aggtatttca caaagtactt tagttaaata tgaaaaaggg tcaagaaaaa tccctaaaga
   113941 cgtagataat actctaagca agattctgaa tatagaaact ctaataaaag atgaggaaga
   114001 taagattgag attctcatcg gtaaactaat tgcatacaga gatatgaata agttacttaa
   114061 caaagagtta gctgataata taggaattag tgaagtatta ctcagttatg tgttaaatag
   114121 aaagagaaat ccgtcaaaag aaatgcaaaa aaaaatcgat atttttctat taagtaatga
   114181 aaaagaaatt ttgaaggaga ttaatcgtga tagtgagata tttagtttat caaaagacga
   114241 taaaattgtt atgggaaaaa gaatacgaga agtccgaaag aaccgggaag agacgcttga
   114301 aaaatttggt aaaaacttta ccatctatac aggcaaaaat gttataagta gatgggaaaa
   114361 aggtattaat attccagata ttgaaaaact aatgaatatt gcctatcttg gtaaggtcac
   114421 tgttccttac cttatgtacg gagaagatta taagaatata cttccaaaag atgaaagagt
   114481 tagcgatttt aaaaagataa attcttttag tatgggatta aggatgagga aaattaggaa
   114541 agattattat ctagaaaggg aggagtttgg aaagcttttt tctccttcaa tttcaaaatg
   114601 gtccatagat aggtatgaga atggtagaga tatacccaat actaatcgaa ttatccaata
   114661 tgcttatata ggaaatcttt ctttagaatt tttgatttac gggatataaa aatagtttac
   114721 gtcgatactt attaagttat cgacgttttt tattatagaa aaaatttata aaaggaggtt
   114781 tcttgatatg ataagaaaca aaaagggttt gttgaaagtt gctttactgg cagctttttt
   114841 ctaagtaact ttttgacaag ctcaatggca acacaagcgg aagaagtaat acaagaacta
   114901 actgttatga ataacagcta gataaaatgg gctatgtatg ttatccaaca atttagtaat
   114961 cactagaagc ctagcgaaag ttcgcttgat aagttataga tgagtattga tttgtagtgt
   115021 ttccaaatca aaagggtaca gagccacatg aaatatgttg tcctctgcag cgtcaggatg
   115081 ggaatccata ccatggagat tcggagatct gtgagggaat acttacgtgg ttaatatgtc
   115141 agttgtgttg gacgtagtaa tggtcatagt gggtagattg actgaattct tatatgagaa
   115201 aatgaaacca acaagaagat agggttattt atacagaatt ggggatcttt tctggaaaag
   115261 taatcggata atgcgagatt cgtacggtct tttttaaatt tctctaaaaa aaacggcatc
   115321 gaatgggttg ggtgattgat aagcaatgaa tcatagactg atatagaata aaaaatgatt
   115381 ctatttatct ttttacatct atcgtttttt caagtagccg caaattatat cagcattcaa
   115441 caatttttgt atacaagttc tagtaaaagg atctgaagaa aaagttggtt gttgtgaaga
   115501 taaaaaaaga atcaggtttt actgagtttt tcttcaaaat ttgtgttatt aggttaggac
   115561 aaagataaga agaagtatga agatttaaaa aaggaatgag gaaaagatag taatttccga
   115621 ttattggagt tttttta
//
LOCUS       DAZKOW010000002        93137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000002 DAZKOW010000000
VERSION     DAZKOW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 93137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.002"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..420
                     /locus_tag="ACG912_000111"
     CDS             <1..420
                     /locus_tag="ACG912_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFQ0511466.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(504..1226)
                     /locus_tag="ACG912_000112"
     CDS             complement(504..1226)
                     /locus_tag="ACG912_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0511467.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(1216..1953)
                     /locus_tag="ACG912_000113"
     CDS             complement(1216..1953)
                     /locus_tag="ACG912_000113"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0511468.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1965..2957)
                     /locus_tag="ACG912_000114"
     CDS             complement(1965..2957)
                     /locus_tag="ACG912_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0511469.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2972..3715)
                     /locus_tag="ACG912_000115"
     CDS             complement(2972..3715)
                     /locus_tag="ACG912_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0511470.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3789..3893)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3956..5278)
                     /locus_tag="ACG912_000116"
     CDS             complement(3956..5278)
                     /locus_tag="ACG912_000116"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0511471.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(5265..6230)
                     /locus_tag="ACG912_000117"
     CDS             complement(5265..6230)
                     /locus_tag="ACG912_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0511472.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(6227..7765)
                     /locus_tag="ACG912_000118"
     CDS             complement(6227..7765)
                     /locus_tag="ACG912_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFQ0511473.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(8181..8468)
                     /locus_tag="ACG912_000119"
     CDS             complement(8181..8468)
                     /locus_tag="ACG912_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511474.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8602..9270
                     /locus_tag="ACG912_000120"
     CDS             8602..9270
                     /locus_tag="ACG912_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0511475.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9561..10301)
                     /locus_tag="ACG912_000121"
     CDS             complement(9561..10301)
                     /locus_tag="ACG912_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511476.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(10431..11378)
                     /locus_tag="ACG912_000122"
     CDS             complement(10431..11378)
                     /locus_tag="ACG912_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0511477.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11507..12271)
                     /locus_tag="ACG912_000123"
     CDS             complement(11507..12271)
                     /locus_tag="ACG912_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0511478.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(12275..12664)
                     /locus_tag="ACG912_000124"
     CDS             complement(12275..12664)
                     /locus_tag="ACG912_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511479.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12799..13341
                     /locus_tag="ACG912_000125"
     CDS             12799..13341
                     /locus_tag="ACG912_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFQ0511480.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13489..14121
                     /locus_tag="ACG912_000126"
     CDS             13489..14121
                     /locus_tag="ACG912_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511481.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(14255..14380)
                     /locus_tag="ACG912_000127"
     CDS             complement(14255..14380)
                     /locus_tag="ACG912_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFQ0511482.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(14514..15407)
                     /locus_tag="ACG912_000128"
     CDS             complement(14514..15407)
                     /locus_tag="ACG912_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFQ0511483.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEIAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(15589..16515)
                     /locus_tag="ACG912_000129"
     CDS             complement(15589..16515)
                     /locus_tag="ACG912_000129"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFQ0511484.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACG912_000130"
     CDS             complement(16600..17361)
                     /gene="pflA"
                     /locus_tag="ACG912_000130"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFQ0511485.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACG912_000131"
     CDS             complement(17605..19836)
                     /gene="pflB"
                     /locus_tag="ACG912_000131"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFQ0511486.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACG912_000132"
     CDS             complement(20113..22563)
                     /gene="parC"
                     /locus_tag="ACG912_000132"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFQ0511487.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACG912_000133"
     CDS             complement(22575..24620)
                     /gene="parE"
                     /locus_tag="ACG912_000133"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFQ0511488.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24786..25220)
                     /locus_tag="ACG912_000134"
     CDS             complement(24786..25220)
                     /locus_tag="ACG912_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFQ0511489.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25347..25985
                     /gene="plsY"
                     /locus_tag="ACG912_000135"
     CDS             25347..25985
                     /gene="plsY"
                     /locus_tag="ACG912_000135"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFQ0511490.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(26117..26992)
                     /locus_tag="ACG912_000136"
     CDS             complement(26117..26992)
                     /locus_tag="ACG912_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFQ0511491.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACG912_000137"
     CDS             complement(27074..27865)
                     /gene="codY"
                     /locus_tag="ACG912_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFQ0511492.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACG912_000138"
     CDS             complement(27883..29283)
                     /gene="hslU"
                     /locus_tag="ACG912_000138"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFQ0511493.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACG912_000139"
     CDS             complement(29297..29845)
                     /gene="hslV"
                     /locus_tag="ACG912_000139"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFQ0511494.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACG912_000140"
     CDS             complement(29882..30787)
                     /gene="xerC"
                     /locus_tag="ACG912_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFQ0511495.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACG912_000141"
     CDS             complement(30985..32361)
                     /gene="trmFO"
                     /locus_tag="ACG912_000141"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFQ0511496.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACG912_000142"
     CDS             complement(32330..34408)
                     /gene="topA"
                     /locus_tag="ACG912_000142"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFQ0511497.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="ACG912_000143"
     CDS             complement(34530..35387)
                     /gene="dprA"
                     /locus_tag="ACG912_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFQ0511498.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35443..36210)
                     /locus_tag="ACG912_000144"
     CDS             complement(35443..36210)
                     /locus_tag="ACG912_000144"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFQ0511499.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="ACG912_000145"
     CDS             complement(36203..37063)
                     /gene="ylqF"
                     /locus_tag="ACG912_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFQ0511500.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            37323..37721
                     /locus_tag="ACG912_000146"
     CDS             37323..37721
                     /locus_tag="ACG912_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0511501.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37958..38500
                     /locus_tag="ACG912_000147"
     CDS             37958..38500
                     /locus_tag="ACG912_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0511502.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="ACG912_000148"
     CDS             complement(38583..39134)
                     /gene="lepB"
                     /locus_tag="ACG912_000148"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0511503.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(39286..39513)
                     /locus_tag="ACG912_000149"
     CDS             complement(39286..39513)
                     /locus_tag="ACG912_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFQ0511504.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="ACG912_000150"
     CDS             complement(39510..40028)
                     /gene="msrA"
                     /locus_tag="ACG912_000150"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0511505.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(40048..40761)
                     /locus_tag="ACG912_000151"
     CDS             complement(40048..40761)
                     /locus_tag="ACG912_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFQ0511506.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40764..41660)
                     /locus_tag="ACG912_000152"
     CDS             complement(40764..41660)
                     /locus_tag="ACG912_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0511507.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41818..42660)
                     /locus_tag="ACG912_000153"
     CDS             complement(41818..42660)
                     /locus_tag="ACG912_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0511508.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42792..43445
                     /locus_tag="ACG912_000154"
     CDS             42792..43445
                     /locus_tag="ACG912_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFQ0511509.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43508..44035)
                     /locus_tag="ACG912_000155"
     CDS             complement(43508..44035)
                     /locus_tag="ACG912_000155"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0511510.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(44055..45002)
                     /locus_tag="ACG912_000156"
     CDS             complement(44055..45002)
                     /locus_tag="ACG912_000156"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFQ0511511.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(45014..46897)
                     /locus_tag="ACG912_000157"
     CDS             complement(45014..46897)
                     /locus_tag="ACG912_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0511512.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(47060..49138)
                     /locus_tag="ACG912_000158"
     CDS             complement(47060..49138)
                     /locus_tag="ACG912_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0511513.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(49151..49426)
                     /locus_tag="ACG912_000159"
     CDS             complement(49151..49426)
                     /locus_tag="ACG912_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511514.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49788..50243)
                     /locus_tag="ACG912_000160"
     CDS             complement(49788..50243)
                     /locus_tag="ACG912_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511515.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(50397..51605)
                     /locus_tag="ACG912_000161"
     CDS             complement(50397..51605)
                     /locus_tag="ACG912_000161"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFQ0511516.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51717..52049)
                     /locus_tag="ACG912_000162"
     CDS             complement(51717..52049)
                     /locus_tag="ACG912_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0511517.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52197..53072
                     /locus_tag="ACG912_000163"
     CDS             52197..53072
                     /locus_tag="ACG912_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0511518.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(53154..53735)
                     /locus_tag="ACG912_000164"
     CDS             complement(53154..53735)
                     /locus_tag="ACG912_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFQ0511519.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(53741..55000)
                     /locus_tag="ACG912_000165"
     CDS             complement(53741..55000)
                     /locus_tag="ACG912_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0511520.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(55002..56048)
                     /locus_tag="ACG912_000166"
     CDS             complement(55002..56048)
                     /locus_tag="ACG912_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511521.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(56283..56558)
                     /locus_tag="ACG912_000167"
     CDS             complement(56283..56558)
                     /locus_tag="ACG912_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFQ0511522.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(56801..58111)
                     /gene="der"
                     /locus_tag="ACG912_000168"
     CDS             complement(56801..58111)
                     /gene="der"
                     /locus_tag="ACG912_000168"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFQ0511523.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="ACG912_000169"
     CDS             complement(58293..59522)
                     /gene="rpsA"
                     /locus_tag="ACG912_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFQ0511524.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(59657..60337)
                     /gene="cmk"
                     /locus_tag="ACG912_000170"
     CDS             complement(59657..60337)
                     /gene="cmk"
                     /locus_tag="ACG912_000170"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFQ0511525.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(60386..61021)
                     /locus_tag="ACG912_000171"
     CDS             complement(60386..61021)
                     /locus_tag="ACG912_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFQ0511526.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(61087..62490)
                     /locus_tag="ACG912_000172"
     CDS             complement(61087..62490)
                     /locus_tag="ACG912_000172"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFQ0511527.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(62477..63508)
                     /locus_tag="ACG912_000173"
     CDS             complement(62477..63508)
                     /locus_tag="ACG912_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0511528.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            63539..63760
                     /locus_tag="ACG912_000174"
     CDS             63539..63760
                     /locus_tag="ACG912_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFQ0511529.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(63925..64662)
                     /locus_tag="ACG912_000175"
     CDS             complement(63925..64662)
                     /locus_tag="ACG912_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFQ0511530.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(64815..66155)
                     /locus_tag="ACG912_000176"
     CDS             complement(64815..66155)
                     /locus_tag="ACG912_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0511531.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(66392..67915)
                     /locus_tag="ACG912_000177"
     CDS             complement(66392..67915)
                     /locus_tag="ACG912_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0511532.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(68293..68904)
                     /locus_tag="ACG912_000178"
     CDS             complement(68293..68904)
                     /locus_tag="ACG912_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0511533.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(69027..69152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(69234..69950)
                     /locus_tag="ACG912_000179"
     CDS             complement(69234..69950)
                     /locus_tag="ACG912_000179"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0511534.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="ACG912_000180"
     CDS             complement(69956..70525)
                     /gene="scpB"
                     /locus_tag="ACG912_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFQ0511535.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(70509..71279)
                     /locus_tag="ACG912_000181"
     CDS             complement(70509..71279)
                     /locus_tag="ACG912_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFQ0511536.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(71276..71647)
                     /locus_tag="ACG912_000182"
     CDS             complement(71276..71647)
                     /locus_tag="ACG912_000182"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0511537.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="ACG912_000183"
     CDS             complement(71666..72553)
                     /gene="xerD"
                     /locus_tag="ACG912_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFQ0511538.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(72649..73122)
                     /locus_tag="ACG912_000184"
     CDS             complement(72649..73122)
                     /locus_tag="ACG912_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0511539.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(73227..74090)
                     /locus_tag="ACG912_000185"
     CDS             complement(73227..74090)
                     /locus_tag="ACG912_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFQ0511540.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(74286..76385)
                     /locus_tag="ACG912_000186"
     CDS             complement(74286..76385)
                     /locus_tag="ACG912_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFQ0511541.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="ACG912_000187"
     CDS             complement(76605..77714)
                     /gene="rpoD"
                     /locus_tag="ACG912_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFQ0511542.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(77738..79579)
                     /gene="dnaG"
                     /locus_tag="ACG912_000188"
     CDS             complement(77738..79579)
                     /gene="dnaG"
                     /locus_tag="ACG912_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFQ0511543.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVRNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(79761..82076)
                     /locus_tag="ACG912_000189"
     CDS             complement(79761..82076)
                     /locus_tag="ACG912_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0511544.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(82261..83121)
                     /locus_tag="ACG912_000190"
     CDS             complement(82261..83121)
                     /locus_tag="ACG912_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFQ0511545.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(83180..83812)
                     /locus_tag="ACG912_000191"
                     /pseudo
     CDS             complement(83180..83812)
                     /locus_tag="ACG912_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            83979..84740
                     /locus_tag="ACG912_000192"
     CDS             83979..84740
                     /locus_tag="ACG912_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0511546.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            84766..87357
                     /gene="mprF"
                     /locus_tag="ACG912_000193"
     CDS             84766..87357
                     /gene="mprF"
                     /locus_tag="ACG912_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0511547.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(87545..88732)
                     /gene="tuf"
                     /locus_tag="ACG912_000194"
     CDS             complement(87545..88732)
                     /gene="tuf"
                     /locus_tag="ACG912_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0511548.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            89100..89456
                     /locus_tag="ACG912_000195"
     CDS             89100..89456
                     /locus_tag="ACG912_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0511549.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(89697..90701)
                     /locus_tag="ACG912_000196"
     CDS             complement(89697..90701)
                     /locus_tag="ACG912_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFQ0511550.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            90867..91718
                     /locus_tag="ACG912_000197"
     CDS             90867..91718
                     /locus_tag="ACG912_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0511551.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            91735..91980
                     /locus_tag="ACG912_000198"
     CDS             91735..91980
                     /locus_tag="ACG912_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511552.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            92167..92871
                     /locus_tag="ACG912_000199"
     CDS             92167..92871
                     /locus_tag="ACG912_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511553.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT        25865 a        19521 c        15980 g        31771 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacaac ctgttgtaca agaaacggtt
      121 caaacggaaa aacaagcagc gcctgttgct gaaacacaac ctgctcctgc tgtaacagaa
      181 actgctgcta ctccagcaag cacaagctct gcaaaagaat ggatcgcaca aaaagaatca
      241 agcggttctt acacagcaac gaacggacgt tacatcggac gttaccaatt agattcttct
      301 tacttaaatg gtgactactc tgctgcaaac caagaaagag tagcagaaca atatgttact
      361 tcacgttatg gttcttggga agcggcgaaa gcattctggg aagcaaacgg ttggtactaa
      421 gattcattta ttaatcaaac agtttacttt tatacaaaga aagccaaaaa gaggtgcgac
      481 actagttgtg tcacatctct tttttattta cctaatctaa tgaaacgttc caagttttga
      541 taagtatttt ttttaacttc ttcaactgat agctctttga tctcagcgat tgtctttaac
      601 tgttcttcaa gcagtgagac ataatctttt tctgaaaaaa tcccccctaa agcccactct
      661 gctgcatgta gtccatccgt ttcaataagt aagtggtcga ttgggactgc tttagctagt
      721 tgccagactg ctggattctt tttcaaatca agaccaatgc tgaaataaca gcctaaatca
      781 atatagtctt gttgatagtc caaagaagaa taccaatgga tcaaatagtg attagggtaa
      841 gcttggatat aatttaagat ctctttttcg catccttttg tgtgcagtac aacaggtttc
      901 tcatttatgg ctgcaaaagc aagctgtcgc tcaaatactt ttttttgtgt agtcatcggt
      961 acattcgtcc aaatattatc taaccctatt tcaccgatga tccttgcttt tttcaaaaaa
     1021 ggctcaactt gttcaaacgt atagctatct gctttccaag gatggatacc gtaacttaag
     1081 gcttgagtct tacttgctgc aagctgtctg ttttccttcc attcttctgg cgaatcacaa
     1141 ttgatgatcg tcaaaatcgt gtgttcctgc tgaaggagtg ctaaactctg actcccatct
     1201 aaatgacaat gagcatcact taacatctaa ttcacctgcc aataactgat aaatcgtttg
     1261 cttcaactct gattgctgca gtagccattc acgtgaccgt cgtttttcag gcacttgaat
     1321 ctcagcaatc acatgtgccg gacgggcgga aagcacaaga atcctgtctg ataaataaat
     1381 tgcttcttcg atatcatgcg tcacaagtaa agtagtccgc tttgcttttt taggcagctt
     1441 caacatccag tcttgcatct gccctctggt catagcatct aacgctccaa atggttcatc
     1501 aagaagcaag taagaagaag gattcatgat tgttcgtaac aaggccgttc gctgcttcat
     1561 cccgccagat aattgttccg gcaaaaaatg ttcatagccg tttaacccaa agatatccag
     1621 ataatccctt acttgttctt cattgattgt tagatgattg attttttgat ataacgtaac
     1681 gttttgatag acgctcagcc aaggaagtaa catatcttct tgcggcatat aactaaatgc
     1741 tgtatggaga ccagttactt tttgatcgtc tactttgatc gttccagaag tcataggctg
     1801 gactcctgtt aaagttttca gcaatgttga cttaccggct ccgcttggcg cgacaatcga
     1861 tgtaaaggta tctttaggga tagtgaaatg gatgtcttct aatattttca ggttttcaat
     1921 gactacattc aagtggctga tttggattga catatctatc actcctattt tataaactga
     1981 ttagtgaata gttcttgcga aggtacatct tgcttgatca atccgttttc ctgcataaaa
     2041 gctgtataat tgcgccaaac tttttcttcc atcaatcccc aattctcagg atcatccgta
     2101 taattttttg acaagaatgc ttgtgattct tctaaaaacg tccgatcatt ttcttttgca
     2161 tatttttgca acagtttagc actgtcattt ggatccttaa tagcttcctg atagcctttt
     2221 ttcgtggcat ccataaaaga ctgtaccaat tctggatcac ttttgatcag ttgatcattc
     2281 gtaataatta ccggagtata gtaatctaac cgttcatcta attctttcaa tgggatatag
     2341 tttagttcaa tacccgcttc ttttgctttg atcatatccc aaccttcaaa gtaccattgg
     2401 atatctgagg attttcctaa gctttctggc ccttctccat tgcttcctac cattgtcagt
     2461 tttgaaaagt ccccgccgtc tttttccatc acagctttta agacagcttc ttcacttgga
     2521 ctttgccaac ctgcatatgt ttttccttca aaatcttttg gtgaatggat gttactgtct
     2581 gatagggtgg cgaatccaga agtattatgt ttgatcactg tagcaattgc tttgactggt
     2641 atattctgtc catctgcgtg agcataagtg acatcttctt gataacttac accaaattgt
     2701 gctttgtccg cacctactag accaatactt gtcgaattat ctcctggttc gatgatttgg
     2761 acatttaaac cagcctcttt ataatagcct ttttcttttg ctaggtagat gccagtatga
     2821 ttagtattcg gtacataatc caaaacaagt gtcacttttt tcaaatcctt tatttcttgt
     2881 ttgctacttg tttcttttgc agtgccacaa gcggataaaa gcaacagtag cggtaataaa
     2941 agccataatt ttttcatttg atccccttct cttaaaaaat ataaagcata cgataatacc
     3001 cttttttcaa taatgaacaa gcgccattca gcaataaact caatagaatg acacaaatga
     3061 tcgcagcaaa aactttatcg atctggtagc tgttttttac gcgtatcatg taatacccta
     3121 agcctgcttg agcgcttagc cattctccaa cgactgctcc gctgacacaa taagtagcag
     3181 ccacttttaa accggaaaag aagccggata aacccgctgg tattttatat attcgataaa
     3241 tcttccctgt atctgccccc atcgttttca aaaagatgat ttgctctttt gatactttcc
     3301 tcaatgtatc cgaaaaagaa actacgatcg gaaaatagct tatcaagaca acaaggatca
     3361 cttttggaag cataccgaaa ccaaaccata gggtaagtaa tggccctaac accatgattg
     3421 gcaacatttg tgaaatcact agtaacggat aaaaagacat ttctaaaaac ttccaataat
     3481 ccattgccaa ggcagtaata aaagaaagaa cgctagctat taccaaacca agcactgctt
     3541 catttaatgt cgtgacagaa tgggaaagta atacttgtgc atctgccgct aatgcagaaa
     3601 atacagcagc aggtccgggt aaaataaagg caggtatttt ccaaactatg acaacaatct
     3661 gccaaataat taaaatgaat gccatactga ctgctggata aagatttttc ttcattttgt
     3721 tacctccttt cttctaatat gaataacaaa aaacgcttga aatgggcacg acatagtaac
     3781 ccgcttcaag cgtctgctat gtttctccct tcaccagtat taactgacag gttcaatggg
     3841 tctaagttca caacttactc tcatgtcgtc tacgacatcc ccagaaacag gtattcattt
     3901 tttaaagtgt agcacaagaa ggaaaaagca acaagcgctt tagttatcat gctgattatt
     3961 tcaataaata ggagagatca cttgccaatg tcggataccc catgatatag ctatccaact
     4021 cttttttagt caatttttga ttgatgacga gtgtcaaatc atcgatcaat tcgtctgctt
     4081 gctcgctaat cactgcagca ccaatcaaat aattttgctg gtcaaaggtc aattcagcct
     4141 tagcaactgg ttcattgaca cgatgatagg tgaaccaact ggtaagatcc atctctacca
     4201 cttgatctga cgaagaggcg tgactttttg taactcctac ttcagcaagt ttaggactgg
     4261 cgtagacgat cgtaggtata atcggatatt tgataggttc gcttgttgca tccgttatcc
     4321 gcttagctac ataatttcct tcaaaagtgg cgactggtgt caattttggg gttttccttg
     4381 aaacaatatc tccacacgca aaaatttttg gattgctcgt ttgcagataa tcattgactg
     4441 caattccgtg tttatcaaag acgatattcg cttgctcaag tgctaaagat tctgtattcg
     4501 gctgtctccc agtcgcacaa aaaatcatat ctgctactaa ttccgtctct tttccgacta
     4561 aacgatagcg agtaccttct gaaatgatct tttgggtttc aacaccaaaa gcaaactgaa
     4621 tgccagatgc ttccatttga tggacggctt cctctaccaa agacgcctca aattctttta
     4681 atggccgttg attatgatgg acaatggtta ctttgctccc tgcagcattg gcaatcgtag
     4741 ctagctcaaa agcaatataa ccaccaccga taaaaatgat ttctttaggc agtacaggca
     4801 atgataaaaa gtctgcacta gttttcaagt attcctttcc ctccactggt aagatcgtag
     4861 gtctttgccc ggttgccaaa actaagtaat ccgcatggaa gacttcttcg ttgacttcaa
     4921 tagatgaatc atccaaaaaa cgagcggttc ctgaaagatg atcgatttca gcttctgcta
     4981 attgtttttt tcggctttct gggactggat ccgtaaatgt tcgtttgaat ttttgaagtt
     5041 cttcccagtt ggcagttggg atctcgttaa aaccttttcc actcaattgt tttacccgat
     5101 tacgtgcctc tactgcactt aacaagactt ttttaggatc acatccacgg ttaggacaag
     5161 tcccgcccca tagatcttct tcgaccacta gtactgtttt acctgcttct ttcaatccat
     5221 aagcagcact taagccgctc acacctgaac cgatgatgat tgcatcatat gttttcattt
     5281 ccttcctcct ctcgacttaa aaaagcaaag atttctttac ttgtcagcga cccaatactt
     5341 ctttgctttt ctactatcac aatgcttgga tacaccgaaa gaagcgaata tacttctttc
     5401 ataggcgtat caaagcaaac ttgttgacga gcattttctg gtatttcatt taagaaatat
     5461 ccattgacga ctaaatcttc tactcgccac tctttcgtct gtttcggttc atcaaagaaa
     5521 tgccgaacgt agtcgttttt aggattcatc aataactgct ctggtgtgtc acattggata
     5581 atttctccat ccttcataaa tgcaatacga tcacctagct tgattgcttc ttccatattg
     5641 tgcgtaacga aaacgatggt cgtgcccaat tcttcgtgta aggacaaaac caattcttgt
     5701 aatgagtttc ttgaaatcgg atctaatgcg ctaaatggct cgtccatcaa aatcacatct
     5761 gggcttgcag caatagctcg gatgatcccg atccgttgct gttctcctcc agacaactct
     5821 ctaggcatac gatctcgata aatatcagga gataagccaa ctttttgaag aagttcatct
     5881 acacggtctg ctcttttctg cttttcccaa cctaacattt cagggatgac ttcaatattt
     5941 tgcttgactg tcatggtcgg gaacaaagcg atttgctgca gcacatatcc catattccac
     6001 cgcattttct gcagattata ttcatttaac gattttcctt tgaagtagat gtctcctgct
     6061 gatggtttag ataatccatt gatcattttc aaagtagttg tttttccact accggaagga
     6121 ccaaccaaaa caaagatttc tccttttttg attgccagat ttaggtcatc gatgacatac
     6181 tttccatcat actttttttg cacatgtttg aactcgatta atttagtcac tgatcttcct
     6241 cctttactaa tccttcattt ttcaggaaga ttcgtgctac ttctgtcggc tttttctttt
     6301 cgacattgac ttggtaattc atatcaatca tttgatcctc tgttattttt ccgcttaatt
     6361 tattcagtgc tttaacgatt tcaggatgtt ctttagcaaa atcttttttc atcagcggtg
     6421 cgccttgata agctgggaat aaccctcgat catcttctag agtcactaga tcgtactcct
     6481 tgagctggct atctgtggaa taggcatcca caatattgac atctccatta ttgattgctt
     6541 ggtaacgcaa acttggctcc atgctttgta cacttgaaaa attcaagcca tatgcttctt
     6601 ggattccttt atatccatct gaacgatcaa taaattccag tgtgaatccc gctttgatct
     6661 gatcagagat agctgccaaa tcggaaatct ttttaagttg atgttcgttc gcaaaagatc
     6721 tcttgactgc caaggcatac gtattttggt aagccattgg ttttaataat gtcatatcga
     6781 attgcttatt caataattct tctgcttcgt catatgtttt ttcttttgtc aaatcagcac
     6841 ttttcattgc ttcaggtacc ttcaccaagc tttctaggac agtacctgtg aactcaggat
     6901 agatatcgat ttgaccttga tccagtgcat tgaacaaaaa actcgtcttg ccaaaattcg
     6961 gttttaattc aacttctgtt tttgtgttgt cttcaatcaa ttctttatac atttggatca
     7021 ggatttcagg ttccgctcct aattttccag caatcgtgat ggtttcattt ttaaacaagg
     7081 cagactgata taaagaaacg ccgccaaata aacacaccac tgctgctaaa gtaatgatgg
     7141 aatatcgagg tttcttatgc tgcaaccagc gaatcagtcc gctgaatata attgcaagca
     7201 aggcagaact gatcgcacca atcaatgtca aaacaggtgt atttcggtca atacctaaca
     7261 aaataaatgt ccctaatcca cctgctccga tcaatgctgc caaagtagcc gtaccgatga
     7321 ttaaaactaa ggcagttcgg atacctgaga taatgacagg taatgcgatg ggaagctcta
     7381 ctttcagcaa gcgtctcatt ctggacatac caaatgctac cgctgcttct tcaatagacg
     7441 ggtcaatttc agctagacca atatacgtgt tttggaaaat cggaagcaaa gcgtaaataa
     7501 caagtgcaat caatgctgga acagttccga tccctacaaa tgggataagt aaacctaata
     7561 aggcaagtga aggaatcgtt tggagcacac ccgtgatctg caataagact tctgccattt
     7621 ttttatgatt ggctgcccaa atcgctaaag gaatagcaat cagtgctgca atcagcaaag
     7681 aaatgagaga aatggacaga tgttgaaatg cagcttccag caactcgcct tttctttcac
     7741 ttaatgtttg tataaaatta ttcatgcgac cttcctttct gttattcttt aagtataaac
     7801 atttctttcc tttttcccaa ctaacttgtc ttgccttaac cttacagcac gaacaaaaaa
     7861 aagaaaaaaa tacctctaaa tatgctgaca aaaaaagaat ccaagcaaca aagtcggatc
     7921 aactttttgg ttctcgtttt ttcactacga ataccataaa aatctatacg aaattgtcac
     7981 ttggaatcag ctatatcttt tttacacaca caacttttat tttaataaaa agaaacagcc
     8041 aataaagtag aaatctcgtg ggggagaaca cctttattgg cctgttcaac gaatattata
     8101 ctgtatcatg acactttttt gccaaaaatg taaaaaatgg ttgttttgac atcataaacg
     8161 tcttgttttc ttatttttac ttatatcacc actttatttt ctatctcttt cactccataa
     8221 agaggaatga gttcttcttt tgactgataa taaacagctt tctcacggtt cttcatcaaa
     8281 aaaagaaaga tgtctttcat agcaagtggt gctttgccgt gttcaaataa aaagataaat
     8341 tgatttgctt tatttatctg gaccattgtc acaggcacca gtttttgttc ttgagccaaa
     8401 aaaactcctt gatactctgt ttttttgggt gattttgtaa gaattgtcaa taagtctgct
     8461 gctcgcattt ttccactcct tatttgataa ataaaaagaa acagccaaaa ataatttttt
     8521 ttcattttcc tcttgaagta agagtataag tcagtataat aaagaagttg ctgaaaattt
     8581 tcaaaagaaa agaggctaga aatgaaaaaa ttaaatttat ttacagaatt gtgcggaatc
     8641 attttaggcg caggaatcta cggattagct gtgacaggaa tcaatattcc atctaagctg
     8701 gcagatggtg gggttactgg tattgctttg cttttgcata atctttttgg attcgctcct
     8761 tctgtcactt cactgctttt taatttacct ttattgctta tctcattgtt tctttttgga
     8821 aaacacgctt ttctccggac gatcgtcgga acatttgcgc tagttttctt tttacattta
     8881 tgggagagcc ttcatataca ttttgctgta gatagtctac tgatcaatag tttgatgaca
     8941 ggtattcttt ctggcattgg ttgcggtctc gtctttcgat tcggcggaag cacaggcgga
     9001 acagatatca tttatcaagc cattgaaaaa tattttcata ttaagattgg caaaagtctt
     9061 ttcttgatta cttttggtat tttagttgtc tccttgcttt atttggatct cacacatttt
     9121 ctgtatacat tacttagctg ttctattcta tcttatacat tgaataaagt gaagtatctg
     9181 gaaattggat ctgttttgcc ccaattggaa gcaaaacaga ctacagaaac atttgaacca
     9241 cttgatcctc ttgaacagtt agagcactga ttcttatttt tcactacttt atttaattta
     9301 cagaaatatc attatgtaaa ctaaaatgat attattcagt aaaatcttct ttataaaaaa
     9361 ataaactatt tttgcaaacg atctgcgaaa atagtttgtt tttttatttt gccattattt
     9421 tcttacttat tgggagaaaa aattcgctta aactcaaaaa aatttgagga tacgatataa
     9481 actttcggat agagtataaa ataaggaaac gccgcagcgt ttcctttcca actgtagccg
     9541 aatataaatt tgtatatcac ttatttaaaa tagctatttt ctcccaaaga gtttccttga
     9601 tcatcgatca atccttgtaa tgagcgttct aagataccat ggcttttttt cgattttttt
     9661 tcttctgact gctttgtttc ctctaacgtt tttgttcgat tgaatgttcg tacagacggc
     9721 tcaccatttc ttttttcttg atacggcgtc ggttctgtat cgaacaacag ataagagtca
     9781 tccgtttttt tcatcgattc gattaaatga tcctcagaaa ttttgctttc tctagggtct
     9841 gggataatcg atgcgggtac atatttagga acaaaataag aacgctcccg caccttcgtg
     9901 tcaacagatg cgtttcgtgt agtatgcttg gactctgtcc ttgtttcact ttgtctgaca
     9961 gaacgagagg aatcaccaaa aagttttttt cgtcctgtcg atgtgatttc tgattggtaa
    10021 tggccggtat aatccggtaa ttttccttta tgcttttcca aattttcctt acgcggagct
    10081 gacttcaatc catttgttgc acgacgcttg cgacggattt tctcggtgtt cgtgttcgtg
    10141 aaatgaaatt ctttcatctg ttcaaacggg gactgattct gttcagtcaa aagatcttct
    10201 tgtccgtcaa ataatactcg cttcttgtcg ttggttaatt ttacgcctaa ctcatcatcg
    10261 tacgcaggaa aacggaaatg ttttcgtctg atttctttca ttcaaaccat ccttctcaaa
    10321 aatggaactt ttctcaataa tagcacagat tcattgtttt cagtagcaat gcgctgatta
    10381 ttttaagaaa atagcgatga atttcataaa tatatatata gtaataagac ttaattcgtt
    10441 gtctctctga taactagttc aggaaaaaaa cgacgataac cttgactcgc ccctttttct
    10501 tctatttttt ttaacaacat ttctccacat acttcaccca tacgtataat atcttgcttg
    10561 atcgttgtca atttaggtga actgatttgg tctaaaaata ccccatcaaa tccaatcaca
    10621 ccaaattctt cgggcaagtg tcctccagca tttataatcc ctcttactgc tcctagtgct
    10681 agccgatctg aactacatac aaaacaagtt ttatctgtaa aatctgccca gttttctgtc
    10741 atatatcttt ctacttctgt cgaatgattt ggaaaccgca agattttact aggaagatca
    10801 acttccttca tcacagataa ataaccagtt tcccttgaaa tttcaaaagg ctcgtccact
    10861 tcgataccga tataaataat cgtttgataa tctctttcta aggcatattg cgttgctttt
    10921 gctgtaccat attcattatc gctgtctaca taatcatatc cttcagaatt ttccccaaac
    10981 aagacaactg gtttttccaa acggtcgatc caatcgaagt ctttttttct gacacctgtg
    11041 atgatgtatc catcacaaga accgacgtcg aaggcatcat ttgtaactaa ttggagagaa
    11101 taatgcgctt taccgatccg tttagcgatt cccatcagca aattcatgta atagggctca
    11161 gttgtgtcga tttcttccag tataaataat ttgatgatct gcgaccgatt cgtgacaagt
    11221 gcttttgcag caatatttgg acggtaatcc agttcagcca ttgctgagaa aactaactct
    11281 ttcaattcgt cagtcacctg ttccggatga ttgatgactc gagagaccgt catttttgaa
    11341 acattggcac gttttgcaac atctgctaaa gtgggcatgc tctcttcctt tctaacatgt
    11401 tgtcattctt actatcaatt ataccaaatt ttcctaacag agtttattat agcccaaaat
    11461 aaaaaacgga aaatcctcta tcgtcatgag tgattttccg tttcaattat ttatactttt
    11521 ttatcaaatt ttttgctaaa aaagtggaag cttcttccat tagtggtatt tgtaccatat
    11581 gtcgctgtgt ttttgaggaa cgaaaaacaa tttcttttcc gtaagactta tgccgatttt
    11641 tttctacaaa tcgttccgtt tgtatataag ggattttttc gtcagcttgt ccatgccaaa
    11701 agaacagagg acgtccagct aacttttccg gctgcaatga caaatcatag ctcttaaccc
    11761 atgaaatcaa atcaaaatag tcttttggta cgacataatt atattccgat gcgcgcctta
    11821 cgatttcttt tccatattcc aaaggtgcag gggaacccat caaacacacg cccgctacta
    11881 tatgaggatg tttagccaac aaggcgcatg ttgtcattcc ccccatcgaa accccgccaa
    11941 ctgcaagctg atcatgtaga agacctcttt tttctaaatg cttgataagt gtgtcaaatt
    12001 caaacagatt tccgtagata ctatcccaaa atgtatagga tggaatagaa gaaaccggct
    12061 gtttgcgctc cccatgattc atcgcatctg gcaggatcac acgaatgccc tgctgcgcaa
    12121 tttttcttcc ttgcgtcaaa actaactcct tacttgtctg ccatccgtga taatagatga
    12181 caacgggaat acgttcctct cttaattctt tcagaacaac ttctaacact ggaatatgat
    12241 ggacagaacg tttcaatata attggattca attgttactc acctattttc attaaatttc
    12301 ctgatcgcca tagcaaccat gctccaacag ttgtctggaa caggttcagt gtaccaaata
    12361 atataaatgc aggactgaat ggctcgggaa ataagcacag agaacaaagt cccaccactc
    12421 ctaatgctag cgaaaggagt ggataaatag aaaaattcaa aaaactggca acgctaccga
    12481 aaatattacc aataaacagt atcaaaaaga aaaaaacgct taaaattaaa ccatctgtca
    12541 gcgacatctg aaaaacaatg ttctctacct caattggctg ttgaatcgaa tagacaccta
    12601 agtatatact gagaaggaga cttagcacgt ggctggcaca aaacacacga ttgaccgttt
    12661 tcattttaat cacctaacct atctttataa ttctattata atcattttat catttttttg
    12721 tatgtataaa gtttcaattt ttcttcactt acgatttttt ttgatattgt tgttatataa
    12781 atgaaaggag ttttacaaat gattgatcca aaaatgttgg ggatgatctt cctcattaat
    12841 tttgcttata tcactctgaa cacgttacgt ttcatgttga caatgaaagg ctatcgactg
    12901 attgctccac ttgtcagtat ggtcgagatc acgatttata tcgtcggctt atcccttgtt
    12961 ttagatcgtt tagataatcc actgaattta tttgtctatg cgttaggtta cgcagtaggt
    13021 atcagtgtcg gaattttgat tgaagacaaa ttggcattag gatacatcat ggtcacaacg
    13081 attttgccag caaatagcgc agaagataaa agtttgccgg gtatactgag agaacacggt
    13141 tatggtgtga cacagacttc tgcaatgggg ctggaaggtg agcggctagt tctcgaaata
    13201 ctttctccta gaaaaagcga acgtgaactg tatcgcctga tcaaagaaat cgaagagcgt
    13261 gcttttatca tttcttatga gccaaaatat atttcaggtg gtttctggac taaaaaagtg
    13321 agaaaacgac aaggaaaata actcacaaac ttaaaaactg tctaatatct aaattaaatt
    13381 ttaaatgtta gacagttttt atttattatt ttgattaaaa aacatcgaca tatactcatc
    13441 aatttgttat tatactttat atacttatca atttaatttg gagatattat ggaaattcaa
    13501 cgtagaacca ctaatttttt acatttaggc tctttttttg caactttgac gatcttattt
    13561 tttctatgtt attcatacct taagacctca catgcgcctt ctagtgcatt aggctggttg
    13621 cttttggatc attctgatat cacagaagcc gaagaaaatc cgtttttttt gattttgtct
    13681 tctctttgcg gcttactgtt ttctgtttat ttcggtgcct atttttttca tcgacatcct
    13741 ggtacgttaa acgaagcttc aagcgtattg attgcattta aaacgttttc ttttttagca
    13801 attctttttt attcgtttaa tcacaatacg cttttcttct ttatcatgaa tggtttagtc
    13861 gtgattttat ccatttttac ttattttatt ttttcactca tttttagcca attgaaatgt
    13921 ccgctcttct ttcgattagt tcctgtcacc tgttttattt acagcttgtt ttcctttttt
    13981 gtccttgctt taatgccact gaccaaccct tcgacgatcc aagtcgcagg aaatctgttg
    14041 gatatgctct tcatcgtcag tctaggtgta tttatgtggc aaagaaaaaa caaacaaaat
    14101 aaaaagcatt ttgaagcctg actattacga cttcataaat gcttttttag ttttaaacac
    14161 ttcaatctaa aagaataacc caagattgga taaattatta atgactcgcc attcgtttct
    14221 tagtgattct ggattatcaa atggacttcc taatctaatc agctagttct tcccataaaa
    14281 tgccatactt gactgaatca taatattgat tatttaaaaa tctaactttt cttatcacta
    14341 cttcctttgt cattccacat ttctcaccat ttttttgcat tcctttgttt ccagaccatg
    14401 tagtgaatct aataggtggt agataggcaa aatactcaaa taattctttt aaccataaag
    14461 gaagagaaag gctcctcctg caagagtcag tataattttt gcttactgct ctcttaggtt
    14521 ttactcacta aaactgttcc atattctttg atataagaaa atgcatgcgg tataaatttc
    14581 ttgctcagct cagaaaagat ttgtcgttta agaaaggcta tttcctccat tgtcaatgaa
    14641 ggatccaatg ttggtaattc aatctcaaaa ttgttttttt cgacaatagg taattctaat
    14701 gtgaatgatt cttcttcctt tatagtattc gtcacaagta gagactgatg gaaaatacta
    14761 ggccggcgat ttttatattt attcttacgt atcctgtcaa cgataacttc tatttgatca
    14821 aaaaagtacg attcatttac gtctccaata agttcggcta attctttgaa tgccttcgtt
    14881 acaggaagct gattcttctg tacaaaaaag taattagcca aagtaagcgg gaagtgttct
    14941 atagaggaaa gtgtgataaa acggtacttc tcatttcctg cagttgtcct cagcaattct
    15001 gtctctcggc tgtttcttac tccataaaaa tacgcttctt tccgttcatc ttcgaaacaa
    15061 tacgcccaaa taacttctcc catcgctaat ttttgttcat caacagagag tctcttcaat
    15121 tgttcagcta tggtttcagc agccgaagtg gcttgttgca agtaatcttt aggatcaaat
    15181 attggaaagt tcaaatgata tcttcgttct tccctcttga ttaatcctgc atcgatcaat
    15241 aaatcaagaa aatgggaaaa tttattttgc gaaaattctt ttttcaattc tcttagaatc
    15301 gtttcttgtt ctcgatgttc atatagataa tcggtcaatg aagaaaaaag cggatcatcg
    15361 gtcagttttt gataaaagtt ttccttattt tttgaataat agttcatata tgttctcttt
    15421 tctatccgtt ttattttact taaaaggctt ttacgtaaaa aggaggaaga acaagagtca
    15481 taattgactg ttattctcct ccatttcttc ggcctttagt atgttctatg tttcttttta
    15541 tatcaatcgc ttatccattc attttgaatg cttcatacta ccatttgatt agctaaatac
    15601 agccgtcaat tgaggtacga cttgtttttt acgtgaaacg acacctttaa gcaaagcacg
    15661 gttgttggtc aaagttgttt ggaaagcttc ttctactttt tcttttcctt taccagcaac
    15721 aagtaattct gaatcactgt ctaagatgtt tgtgacaact aatacgaaca gatcgtagcc
    15781 atttgctttc atttcatctt ccattgctgc ttctagctct gcttgtcgat ccatcacttc
    15841 attaaggtcc actgtattga cttgagcgat acgaacgttg gaactgccca ttgggaaact
    15901 ttttgcatcc aaatcaagaa gaaccgcagc tgatttatca cttagatttg ttcctgcttt
    15961 caacatttca agtccataag tgtttagatc gacttcagcg attgctgcaa gttcttttgc
    16021 agctgcttca tcttcaggcg tacatgttgg tgatttgaac aacaatgtat ctgaaatgat
    16081 agctgagagc atgattcctg cgatttcttt aggaattgtt acttggtttt ctttatatag
    16141 ttttaaaata atcgtacttg tacatccaac tggttctgca cgatagtaca atggattagc
    16201 tgtttcaaag tttgcaatac gatggtggtc tactacagct aaaatattta cttcgctgat
    16261 atctgaaata ctttgttgga attcattatg atccacaagc atgacatctg cattttctgc
    16321 tttagctgac tctactactc gtggtgcaga tagctgaaag tggtctaatg cgtattgtgt
    16381 ttcttcatta ggtgtaccta gagcaactgc ttctgtttct ttacctaatt cattttgcaa
    16441 gtgtgaaaac gcaatggctg ccccgattgc atctgtatct ggattttggt gaccaaaaac
    16501 taaaattttt gacatgtcat tcgctccttt gtgatttctc ctatttaata atagcaaaaa
    16561 aacggaaaga agcaatactt atcttctttc cgatgcgacc tatcgcgtta aatattttgt
    16621 atactcttct gtatgaagca atcgtttagc attttcgaca cgttcttttg taggtggttc
    16681 gatcccttct aatggatatt tcaaaccaag tgtttcccac ttgtattttc ccatcgtatg
    16741 ataaggaaga atctctactc tgtctacatt tgacaaggtt tgaataaagt tgtctagtcg
    16801 gatcaaatat tcatcataat cacttcgctc aggaactagt acatggcgaa tccaaaccgg
    16861 tttgttgatt tttgacaaat attgagccat ttctaaaata ttttcattgt cccaatgcgt
    16921 tagtttttta tgctcttcat tatcgatgtg tttgatgtca aataacagta aatctgtata
    16981 cttcatcaat tcttgaaaac gtgaaaagaa aggttcttca tacgtaaaag gttttccgca
    17041 tgtatccaat gtagtatgca tcttccgctc ttttgctttt ttaaatagtt cgatcaaaaa
    17101 atcgatctgc aatagaggtt ctccaccact aacagtaata ccacctttat ctccccagta
    17161 aggtctgaag cgttctgctt tatctaaaag ttcatctgct gtgtattctt tccctccgcc
    17221 aatattccaa gtatctggat tgtgacaaaa ttggcaacgc atgcggcatc cttgcataaa
    17281 cacgacaaag cgcagtccag gtccgtctac tgaaccgaaa gtttcgatag aatgaacata
    17341 gccgattgtt ttttcttcca tttaagttca catcttttct ttgatttcat tttaagtaca
    17401 agttagctgt cgaagcgaag ctttctgaac aaaatttttc gcaattgccg cttcgttatg
    17461 tgtaaaattt acagggacgg aacatacaag tcctgcccct gtaaatcgtt tctttcaaat
    17521 aagcatactg attattcgat tttgatgcga tggctattca acgtcctttc gacgctaagt
    17581 cgaaaaactt ttttcacgaa ctttttacat tctgtcgtgt gaagttctca tcaagacatc
    17641 taattgttgt tctcttgtca agtcacggaa tttaactgca taaccagata cccggatcgt
    17701 caagttcgga tatttcattg gattttccat tgcatcacgc aataaatcat tactgaatac
    17761 attgacattc aaatgataag ctccttttga gaagtatcca tctaagacat tacgcaagtt
    17821 ttcgattctt gtatcttgtt cttttccaag acctgaagga ttgattgttt gggtatttga
    17881 aattccatcc aatgcacagc cgtaattcat tttagctgtt gagttcaggc tagctaataa
    17941 cccactttgt tccgcattgt aacttggatt tgctcctggc gcaagtggtt ttccagcttc
    18001 acggccatca ggagtatttc ctgtattttt accgtaaaca acatttgaag taatcgtcaa
    18061 taatgaagtt gttggttttg agttgcggta agttttatga cgtttgattt gtcccatgaa
    18121 gtattccaag atccatgaaa ctaattcatc tgcacggtca tcatcatttc cgtatttagg
    18181 gaagtcgcca tccacttcaa aatcaactgc tattcctttt tcattacgga ttgcttttac
    18241 ttttgcatat ttgatcgctg acaagctgtc tgccgcatga gagatcccag caatacctgt
    18301 agcaaatgtg cgttgcaagt cagaatccat caaagccagc tgagctgctt cataagaata
    18361 tttatcatgc atataatgaa tgatgttcaa cgtattgaca tatagttctg ctaaccaatc
    18421 catcatattc atgaagttat gtttcacttc ttcaaagtct agtatttctg atgtgattgg
    18481 acgatattct ggtccaactt gggcgccagt cacttcatcg actccgccat tgatggcata
    18541 taatagtgtt ttagccaagt tagcacgagc gccaaagaat tgcatgtctt ttccgacaac
    18601 tgttcctgat acacagcaag caatcgcaca atcatcagaa ccccattgtt cacgaagctg
    18661 attatcattt tcgaattgaa tcgaagaact ttcgatagcg attctgctag catatgttct
    18721 gaatccttct ggaaggtgtt cagaatacaa aacagtcaag tttggttctg gtgaaggtcc
    18781 catatttgtt aatgtatgaa gcagacggaa atcattcttt gtaactaatg aacgaccatc
    18841 taatcccata cctgcaatgg ataaagtagc ccagataggg aatcctgaga ataattggtt
    18901 atattcaggt gtacgcgcga atttgaccat acgtaatttc attactaagt ggtcgatcaa
    18961 ttcttgtgct tctttttctg tgattactcc attatccaaa tcacgttgga tatagatatc
    19021 aaggaatgct gagatacgtc cgattgacat cgcagcacca ttttgtgatt tgattgctgc
    19081 taaatatccg aagtatagcc attggacagc ttcttttgca tttgcagctg gtttagaaat
    19141 atcatagcca tatttcgcag ccatttcttt catttgcgct aatgcacgat attgttcagt
    19201 gatttcttca cgtaaacgaa tcacttcttc actcattgta tgataaccag tcatatcatg
    19261 atcttttttc ttttgttcca tcaaatagtc gattccatac aatgccacac gacgatagtc
    19321 accgatgata cgtccgcgac cgtaagcgtc tggaagccct gtaatgattt tgtttttacg
    19381 agcagcacgc atttcagcag tataagcgtc aaatacacct tgattatgtg ttttgcgcca
    19441 gtcagtgaaa atatgtgtca attcatcgtt tggtgtatag ccattgcttt ttagcgcatt
    19501 gttagccata ttgattccgc cgaacggcat gaatgcttgt ttcaaaggtt tgtctgtctg
    19561 tagaccgacg attttttcca aatctttatc caaatagcct gcatcgtgtg aagtaatagt
    19621 tgatacgatt tctgtgtcca tatcatagac accgttacgc tcgaattgga tatcatttaa
    19681 cttttgcaat tcatcccata atttttctgt tgcttctgtt gggccttcca aaaagctgtc
    19741 atcaccgcga tattcagtgt agttattttg aataaagtct ctagtgtcta cgccttcttt
    19801 ccatttggta cctttaaatc cattccattg ctccattgta tggacctcct aaatttctgt
    19861 tttatgtaac agtttttacg tgattagtat aacacagcac cctatctttg caagggtttt
    19921 cttgtttttt atttaacaat ttttatgttt tatttacata tatttaaatc aacgttgaca
    19981 taccaacatt taagcattgt gaacactttc atcttttttt gcaaacgtat aaggatttat
    20041 taaaaatgaa tgaaaaagag acgccacctg tattaataca gatggcgtct cttcattaaa
    20101 atactacaac agttacgact cgatgaaaat ctcaggagca tcgataactt cgatgacttc
    20161 tccacctttt ttctcatcca tcgcaaatga tccattcgat gttcgatcac ctatagggaa
    20221 atcagttggg tcaatcgtta actgctgccc tttttgattc atcaaggtta tttcttttga
    20281 tcttccttcc gacatataga taatacggtg cggatttttt ttcagctcac gcatgaccgt
    20341 cactccccgc ttcgcacgtg aagtttggct gatttcttga gcaagcatgc gtttaacagc
    20401 tcctcgctga gtaacgatta cgatcggcgt atcaccttct gctaacacaa gcagcccgtt
    20461 gataatccta tctccatctt tcaaattcat agacttcact ccagctgcct ttgctccaac
    20521 aacgggaaca tctgttaaag gatagcgcaa acccattcct tgatgcgtta ctaagaaaac
    20581 atctaagcct ggctgatctt ctttcagata cacagcaatc agttcatctt cttccgcctt
    20641 tagcttcata cagctgagcg gacggctctt ataggtgcgc catggttcga actctgtcat
    20701 tcgtgtttgc ttgatcaatc cattcttgct gatgaataca aatgttttct ctgcttgaat
    20761 tttttgatat gggaaaacag ctaaaatcga ttcgtctaca gacagattgg agattgtttg
    20821 agaaatgtgc tcaccagcat ctttccattt caaatcgggt agttcatgga cagggcggta
    20881 gatcacattt gctttatttg tgatcagaag aacatggtcc aatgtactca attcacctga
    20941 gtatagcaag tagtcgcctt ctcgcatccc gatttcttct ggtttggaag cactataaga
    21001 acgaaggctt gttcgcttca catagccttc tcgcgtcaca ctgacaatta cgtcttcttg
    21061 tgccactaag acttgcgtat caattttgat ttcttcgatt tcgtcttcga ttgtcgttag
    21121 acgagcggaa gaataatttt ttttcacttc tcgcaattct tttttcataa cagagaaaag
    21181 ctctttgtca tttgaaagga ttttattcaa ctctgtgatt tgcgcaatca agctttcgga
    21241 ttccttttga agctctgtga tatccgtgtt cgtcaaacgg tataattgca atgtgacaat
    21301 tgcttctgct tgttcttctg taaattgaaa gacgtcgact aaattctttt tggcgtcttt
    21361 tttgtcttta ctttcacgaa tcgttgcgat tacttcatcc agaatcgata aagctttcat
    21421 caatccttct acgatatgct gtcttttttg agctttggct aaatcgaatt gactacgttt
    21481 tgttatgact tgctttcgat gctcgatata acttcttaaa atgtctttca gtccaacttg
    21541 atgcggagtc atatggtcga tagcgaccat attgaaattg taattgatct gtagctcagt
    21601 gtttttgaac aaataattca aaattccttg agcattggcg tcttttttca attcgacgac
    21661 aatttgcaga cctgttctat cactttcatc tcgtacttca gctattccat cgattttctt
    21721 attcaaacgg atctcatcca tttttttcac caaggtcgct ttatttactt catatgggat
    21781 ttctgtgatc acgatttgct gtttattacc cttcattgcc tctatctttg ttttcgaacg
    21841 aagaataact tttcctcgtc ctgtttcata agcttttttg atttcatctt ttccttgcaa
    21901 gatccctcct gttggaaagt caggtccagg aatatattcc atcaattttt caaggctcgc
    21961 ttgtggatga tcgatcatat agattgtgcc atcgatcact tcagctaagt tatgagtcgg
    22021 gatttctgtt gcatatccag cagatatacc tgtcgatcca ttgacgagaa ggtttgggta
    22081 tcgagctgga aggactgttg gttctttttc tgtatcatca aaattccaca cgagatcgac
    22141 tgtttctttc tcgatatctg ctagcatctc tccacttaac ttggataatc tggcttcagt
    22201 ataacgcatc gcagcaggtg gatcaccgtc catactcccg ttattcccgt gcatttcgat
    22261 cagtacttcc cgcaacttcc agtcttggct catgcggacc atcgcttcat aaatactgat
    22321 gtcaccatga ggatgataat tccccatgat atttccgaca gattttgctg atttacggaa
    22381 tcctttgtca tacgtattgc catctttgtt catggcaaat aatatccggc gttgcactgg
    22441 cttcaatcca tcacggatat caggcagtgc acgttcttgg ataatatatt tggaataacg
    22501 tccgaagcgg tcacccatca cttcttctaa agttaattct tgtacttctt gtcgattttc
    22561 catcaggtga cctcctattc tacttcaaat aactgattgt tttcattttt atctgctcgt
    22621 tcttcatcta ataaatcatt agaaactgaa ggagagattt cagtatcctc ttttttatct
    22681 aagatactgc catcttcttc gagcgtaaat tgcacgtgat tttcgatcca ttttcttcga
    22741 ggttctactt tgtcacccat cagtgtggtt actcgacgtt cagcttgcgc agcatcatcg
    22801 atacggacgc ggatcaacgt acgggaagtc ggatccatcg ttgtttccca caattgttcc
    22861 gcattcatct ctccaagtcc tttatatcgc tgcagcatat atcctttgcc gactttttta
    22921 atcattgctg ccaattcgtc atctgtccac gcatattcaa tgacttgctt ctttccttgt
    22981 cctttggata ctttatataa aggcggtaac gcgatatata ctttcccagc ttcaatcagc
    23041 ggtttcatat aacgataaaa gaaagtaagc agtaatactt gaatatgggc gccgtctgta
    23101 tccgcatcgg tcatgatgat tactttgtcg taattacagt cttcgataga aaactcagga
    23161 ccgactcctg caccaatcgt atagatcatc gtattgattt cttcattttt caagatatct
    23221 tgcattttag ctttttcggt attgatgact tttcctcgta aaggtagaat tgcttggaac
    23281 tttctgtctc ttccttgttt agcagatccg ccagcagaat ccccttcgac taaatacaat
    23341 tcatttttct tagggtttcg ggattgtgcg ggtgtcaatt ttcctgaaag aagagattcg
    23401 ccttttttac gcttcttccc atttcggctt tcttctctgg ctttacgtgc tgcttctctc
    23461 gcttctctgg ctttgattgc tttacggacc aacatctggc tcatttcgct attttcctgc
    23521 aaataaaagc ccatttgttc actgatgacg ttatctacta cggtacgagc aactggcgta
    23581 ccaagttttt cttttgtttg cccttcaaat tgcagaagat tttcaggtac acggatagaa
    23641 agtacagcag ctaacccttc tctgaaatca ctaccttcca gattcttgtc ccgttctttc
    23701 agcaaaccaa cttttctcgc atattcatta taagatttcg tcatcgccgc tttcatacca
    23761 gcttcatggg ttcccccatc ttttgtccga acgttattga caaaagataa aacattttct
    23821 gaataaccgt cattgtattg ataagcgacc tctacttcga taccttcttt ttctcctgaa
    23881 aaataaacaa ctggtgtcaa tgtatctttt tcttcattca gataatcaac aaattctttg
    23941 attccttctt cgtaatgaaa aatttccttg actggttctt ctccacgaag atcagaaaga
    24001 gagattttaa cgccttttag aagaaaggca gattctctta accgttcggc taaaatttca
    24061 tatgaaaatt tcgttgtact gaatattgta tcatctggta aaaaatggac agtcgtcccg
    24121 tttgcttttt ttgtttttcc tatctttttc aaagtaccgt caggtttacc gccgttttta
    24181 aatttctgct gatactctac gccatctcta acgatcgtta caattagcca tttcgataag
    24241 gcatttacca cactggctcc tacaccgtgc agtccaccag aagttttgta ccctccttga
    24301 ccaaactttc cgccagcatg cagcacagta aaaatcactt caactgttgg tattccggaa
    24361 gcatgcattc ctacaggcat cccacgtccg ctatctgcaa cagttatact attgtcttca
    24421 tggatggtca catcaatctc actgccataa cctgataatg cttcgtcaac tgcattatcg
    24481 acgatttcat agactaaatg atgcaagcct cggctgtcag tggatccaat gtacatccct
    24541 ggtctttttc taactgcctc taatccttcc aatacttgga tggacgagtc attgtattcg
    24601 ttgtttactt tttttgccaa ggcaaacgct ccttcaataa tactttgcgg caaaaccgca
    24661 ccacgattat catactagaa aaggtcgtga aaaaaaagag ggtagacgag aaatcaattt
    24721 taattatctc ctaaccgctt tttcgacatt cccctctatc gatcatctct tctttgtggt
    24781 catttttatt tttggttttc tttcaactta ccaagttcaa tcttaataca acgatccatt
    24841 acgatatctg tacgtcctgc ttttcttagc atctcagctg cttctttgtt ttcaatccct
    24901 aattgtgccc agaacacttt agcatcagtt ttcaaaaaat cacctgctat ttcaggtaaa
    24961 aattcactcc gtctaaatac atctacgata tcgattggat catcgatttc tgtcaaattc
    25021 gcatagactt tctcgcctag aatttcttca ccagccaata atggatttac tggtatgatt
    25081 ttatatccat tgtcttgcaa tacctgtgca accatgtaac ttgttcgttc tggtttattg
    25141 ctcaagccta cgacggcaat attttttgct tcttccaaat agttgaaaat cgtttcttgt
    25201 gttggatttt ttacagacat ttctgccacc ccttgctttt ctttttattc catctattat
    25261 acttgtatct ttcatttttt caattgttcc tgttacctat tcatgctaaa ataaaggata
    25321 gttttcaact tgaaagaggg taaaaaatga aattgatctt attacttatt ttagcatatc
    25381 ttttaggttc tattccttcc ggtgtctgga ttggcaaatt gtttttcaaa aaagatatcc
    25441 gacaacacgg gagcggtaat actggaacga ccaatacatt tcgtgtatta ggcaaaacag
    25501 ccgggattgt tgtcctgctt atggatattt taaaaggtac attggctact tgtttgccga
    25561 tgatcttcca cctttctggg attaaccctc tttggtttgg ggtatgcgcc attcttggcc
    25621 atacatttcc gatttttgct gggttcaaag gaggaaaagc tgttgcgaca agtgcaggaa
    25681 tgcttttagg atttaatcct gtattttttg tttattcttg tatcatcttt atcgttagtt
    25741 tattttgtac aagtatggtg agtttgagca gtatgatcag tgcagtattg atcactttat
    25801 ctacgatcgt ccttccttat gtagctcctg tgattttggc taagcccaat tggttactga
    25861 cgatcattgc ctttttagtt tcttccttta tcttttatcg gcataaagac aatatcaagc
    25921 gtatcagaaa ccatactgaa agtcgaatcc cgtttggttt gaatagcagc aagaaaaaat
    25981 cctgatactt tttcattcca aagagataaa acgggagtgt gacaaatcga tgtcacactc
    26041 ccttattact tacaggatag cttttttaca caccttggta aacggtgttc cctgctaact
    26101 tctcggtcac aacctcttac ttcactgtga tggaatattt tgtcttgaag atttcatggg
    26161 cacctagttg attgatccct actttttcca tcaagtttcc actactgttc accgtgtcag
    26221 cgattccgca ccacggttcg atacaaacga aaggcgcttc ttttggataa ggagaccaga
    26281 tccctacata aggcatatct ttatagctta aagttacgct atgggaagat ttttcacttc
    26341 gaatggcgta cgcatttaga ccccttgttt caaaaaccaa ggcatcttgc tcaaaaagtt
    26401 cacgattcaa agccagattc gtatttgtct gacctaacgt cttttgatcc atatcgatga
    26461 atgggccttt caatggtagg cgtaatcttg atttcaatgg agaaaatgcc agatagaaat
    26521 cttcaaatga caatccttct tcaagaggta cattgaaagc aggatgtcca ccgatagaaa
    26581 agaacatttc tttttcaccg gtatttgcca cttgataatg aaccgtgatg ccgtctcctc
    26641 ctaattcata gctgatcact aattcaaaat caaatggata cttttctaat gtctccggcg
    26701 tactccttaa gatgaaacta gccatttctt gtccatgttc aatgacttca aaatccatat
    26761 cccgagcaaa tccgtgttgc cccatttgat atgttttccc ttgataaaca tactgatcat
    26821 cttttaaacg tccaacaaaa gggaataaaa ccggtgcatg tctgccccaa tacgctggat
    26881 caccttgcca gatatactct aaaccatgtt cttttgattt cagactgatc aattccgcac
    26941 cgtgttcagc gatttcggcg atcagctggt cattttgaat aataacagtc attgccattc
    27001 ctccaacttc gacttacttt ttaaaatggg aaggctggaa caaacatttc tatctgcccc
    27061 agtcccctta cttctaataa ctgtgttttg ctaactcgtc tttaaaacgg gaattcaata
    27121 cttttaaata agttcccttc atgcctaacg aacgagattc aatgattcca gctgattcca
    27181 attttcgtaa agcattgacg atcacagaac gagtgatgcc gatactatcg gcaatattag
    27241 aagcagtcag acgaccttca tctccatcca atgcttcaaa gatagcttgt acagccttca
    27301 attcgctata agataatgtg ttgattgcca tttggacagc cgttgcactc cgtacatctg
    27361 cttcgatgct tcgagacttc tgataaagga tctgcatacc gacaaccgta gctccatatt
    27421 ctgctaagac tagatcgtca tctgcaaacg attgttccat acgagataag ataatcgtac
    27481 ccaaacgttc gccagctcca aagatcggaa cgacagtcgt cagaccaaat ggatatttct
    27541 ctcttgactc taccggaaat gcagtaatat cactagtaat tgggatatta gcttcagttt
    27601 tcatcaactg tgccactgta tcagtataag attcaggaaa ttgtttctca acaaacattg
    27661 ttttgattcg ttcattgttc acatcgtgac gttcattata acctagtaat actccgtcac
    27721 ttcctatgat ataggtgttg ctgtcaagaa taccacctag tgtgacagcc attttatcgt
    27781 aaggcaaatc tgcttgcatg tcaaaagtat tcttttgctg caaaagtgtg tttatcttgc
    27841 gggttttcgt tagcaagtct gtcattgtcg tcctccgatt ttttataaaa tatagcgact
    27901 taaatcttca tttgccgcaa ttgagccaag tttgtcatcc acatatgctt ctgtgatcgt
    27961 tatttctccc atttgcatat ctggtgcttc aaataataga tcttctagta gtttttccaa
    28021 aatggtatgc agtctgcgtg cgccgatatt atctgtgtca cggttgacat cgaaagcgat
    28081 ttgagcgatc cgttcaattg cttcttttgt aaacgtaact gacacatttt ctgtaccgat
    28141 caacgcgacg tattgtttta ctaaagcatt atttggttcg gtcagaatct tcacaaaatc
    28201 ttcagctgtc aaatcatcta attctacccg tatcgggaag cgtccttgaa gttctggaat
    28261 tagatcactt ggtttagaca aatggaaagc tcctgaagca atgaatagga cgtgatctgt
    28321 ttggatgatc ccgtattttg tgtttacttg tgaaccttcg acaattggca atatgtctct
    28381 ttggacgcct tctctggata cttctccaga ttgctgtgat ttagaagtga tcttatcgat
    28441 ttcatcgata aaaataattc cactttcttc cgctaatttg attgcctcac tatggatatc
    28501 agcatccttg actaattttg ctgattcttc cttgatcaat aactctaaag cttctttgac
    28561 tgttaccgtc cgttccactt ttttcttcgg tgtcaaagca cctagagttt catttaggtc
    28621 gatccccatt tgctccaatc cattgttcat cattggtgca ggtttctttt ggtcttcaac
    28681 ttcaatggtt acttcacgac tatccagtaa acccttttgt agttgttcca agactgtacg
    28741 gcgattgatt cgaatatctt ctgtcacttc ttcttgcggt tcagcggtat tttgctgact
    28801 agcattaaaa agattcatca tttgttcaaa ttgattagtg gattgtttct gttctttttt
    28861 gataccaggt accaagattt ttaccaaacg tttttctgca ttttttttag cacttgtgta
    28921 tacacggctg tactgctctt tttctacgat ttggatcgca ttttctacta aatcccgaac
    28981 cattgattct acatctcttc cgacataacc gacttcagta aattttgttg cttcgacttt
    29041 gacaaatggt gcattgacga tttttgccaa acgtcgtgcg atttctgttt taccgacccc
    29101 tgtaggtccg atcatcagca tattttttgg tgtaatatct tgttgcattt tttcatctaa
    29161 ctgcaaacgg cgatagcggt tacgcaaagc aacggcaaca gatttttttg ctgcgtgctg
    29221 tcctacgata tattcatcta attcacttac gatttgtctt ggtgtttgtt ttaattcata
    29281 catatctact cacctcttac agttcttcta cgataatatt atgattagta aagatacaga
    29341 tatctgcggc aatatttaac gcattttctg caatttcttt cgcactcatg tcgttttgag
    29401 catagttttt cattgctcgg gctgcagcca acgcaaaatt ccctcctgat ccgatagcta
    29461 agataccatc atctggtgtg ataacttctc ctgtacctga aacaagaagc atttctttat
    29521 cattcattac gatcagcatc gcttcaagtt tttgcataga ttgctgtgtc cgccattctt
    29581 gagctaattc gacagctgcg cgttgtagat tcccgttata ttcgtttagt ttgccttcaa
    29641 atttttcttc taaagtaaac gcatctgcaa cacttccggc gaaacctaca acaacttctc
    29701 cattgtagat acgtcgtact ttctttgcag tgcctttcat gacaacagat tcacccattg
    29761 ttacttggcc atctccagcc atagcaaatt tcccgtcttt ttcgacggca cagatagtgg
    29821 ttgaatgaaa tggtgattcg accatgtttg tttcctccta aatttgttct tttccttatt
    29881 tttacgctct tggatggaaa gaacgatagt ttttttgtaa actttctttt gttacatggg
    29941 catagatctg ggtagtcgat aaattggcat gtcctagcaa ttcttggact gtccgcaaat
    30001 ctgccccatt atttaataaa tgagtagcaa atgtatggcg cagcatgtga ggatggatat
    30061 cactatccaa actgcttttt ttgatcattt gattcaagac atattcaatt ccagtaggcg
    30121 tgattggttt tccatgatga ttcacaaaaa cgatatcatg ctcttcgtga tattttgtca
    30181 tcaatacctg gcggccttcc ctgaaatatt cttgcagagc atcttgagca aaagaaccta
    30241 gaggagcata tctctctttg ttccctttac catggatcaa catgacctct gaatcaaaat
    30301 caatgtctga taccgacaaa ctgctacact cacttaaacg gatgcctgac ccgtaaagga
    30361 cttccagtaa agcgcggttc ctcaaatcca aaggactatc tcctgctacg ctgtcaaaaa
    30421 gtgcttgcat ttcattttca taaaaaaaac gtggcagtct caaatttttc tttttgagat
    30481 gaacgtaaga aaatggattt tcttttatca cttcttcttt gagaagatac tgataaaacg
    30541 aacgtaggct ggctatcttg cgactgatag aatttctgct atagctagga cgatgatcag
    30601 ctaatgcact caaatagaca cgaaccgtaa aatattccgc cttcagcaga tcagcgtctc
    30661 cagattcctc caaaaaatgt ttgaaatgga gaatgtcttc ttcatatgct tctttcgttt
    30721 tttctgaata tcctctttcg acaatcaaat aacgcaaaaa tttttgttcc cacgtggctt
    30781 ctgtcatttt ctcaccttcc ttttgtccca gagaataatt tagcacagcg tttataaaaa
    30841 gacaaataaa ttgtcagaat ttaaccaata tttctaagtt tgttcacaat tggcgcaatt
    30901 cctcacattt tgtttgagaa attcttaaga aaaacgcaag aaaaagagcg agactatccg
    30961 tctcaccctc ttcatggagt ttccttactc atttgggatt gcagtttttt ctaattctgt
    31021 ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt gcttcatatc ttgctttttt
    31081 gtctcgaatg cgctctggca gttcaggaaa cagaccatag ttggcattca tcggttggaa
    31141 atgttttcct tccgcatgag taatgtagta agccatactt ccaatagctg tttcttgtgg
    31201 aaactcgact ggttccattt cttttgctaa gcgtgcggcg ttgattccag caagtagtcc
    31261 gctcgctgca ctttcaacat agccttctac accagtcatc tgacctgcaa agaacagatc
    31321 ttcgcgtttc gttgattggt aagtcggttt cagcaattct ggcgaattca taaagctgtt
    31381 tcggtgcatc acaccatagc gaacgaattc agcattttct aacccaggaa tcatccggaa
    31441 aacacgtttt tgctcgcccc atttcaagtg agtctgaaag cccacgatgt tgtataaaga
    31501 agctgcagca ttgtcttgac gcagctggat aacagcataa ggacgtttgc cagttttagg
    31561 atcttccaat ccaactggtt tcataggtcc gaaaagcatc gttttgaatc ctcgctgtgc
    31621 cattacttca attggcatac atccttcaaa atatttttct ttctcaaaag atttaagttc
    31681 tgccacttcg gcattgatta gtgcctcatg gaaagctttg aattcctctt ctgtcatcgg
    31741 acagttcaag taagctgctt ctcctttgtc atagcgggac ttcaaatata ctttatccat
    31801 atcgatcgta gctttgtcta cgattggtgc agctgcatca tagaaataaa aaccatctga
    31861 accattgaat gctttgatgc tttctgctaa tggatgagaa gtcaaaggtc ctgttgctac
    31921 tacggtgatt ccttccggta tttcagtaat ttcttcgttt atcactgtta ctaatggatg
    31981 ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg cggtcaacag ctaaggcacc
    32041 accagctggc actgctgttt catctgccga agtgattact accgaattca atcggcgcat
    32101 ttcttctttc aataacccta ctgcattagc caaactgttt cctcttaatg aattggaaca
    32161 tactagttca gcaaaattac ttgtgtgatg cgcctcagtc gatttgactg gacgcatttc
    32221 atataaacgg acagggactc ctgcttgtgc gatctgccaa gcagcttcac ttccggcaag
    32281 ccctgctcca acaacattta ccattttcaa tgaaagaacc tcttttctat tatttttgga
    32341 tgtcttcttc gtaatctcca tttacacaga ctacttgttt accgcctttg actttcttct
    32401 cgaccagata tccgtcacat tttggacaat ttctgcccac tggtttatcc caagaagtga
    32461 attcacattc tgggaaacgg gaacaaccat agaatagtct gtttttctta gattttcgtt
    32521 caatgacttg tccttcatga catactgggc aagtcacacc gatttctttt acgattggtt
    32581 tcgtattccg acaatcagga aaattactgc aagcataaaa cttcccatat ttccctaatt
    32641 ttatcaccat cggatgtcca cagacatcac aatcgaatcc tgctggttca tctttgattt
    32701 gaattttttc gattttttct tccgcattcg tcagttcttt ttcaaacggt ttatagaaac
    32761 gatcaaccac tttgacccac gcttccgtgc cttcttcaat tttatccaaa tctccttcca
    32821 tttctgctgt gaaatggata tcgacgattt gagggaagaa ttcacagatt aatgaattga
    32881 caatttctcc tagttcagtc ggctcaaatc ttttttgtgt caattttaca tagtagcgtc
    32941 tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg ccctaccccg ttttcttcta
    33001 gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg ttgagtaaaa tgttgttttg
    33061 gttcaatatc aacagattgg actacatcgc cttcttcaag ttctggaaga atattttctt
    33121 tctcttcttt gccgtcatct ctaccttcca catacacttg catgaatcct ttgaatttga
    33181 tttttgaacc gttcgcaatg aagatgacac cattttgttc taatgtcact ttcattgtat
    33241 ctaaaatagc tggtgtcatc tgactagcca caaaacgtga ccagatcaat gtataaagtt
    33301 tcaattgatc tttatctaaa tattgtttga gttcatctgg tgtacgaagc acactcgttg
    33361 gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt tttgatcttt cggtgatcat
    33421 gtctagaaaa ttcttttcca tacgtatctt caataaatgc tgcggcctct gcttttgctg
    33481 tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat caagccgact gttccttgtt
    33541 tacccaaagt gattccttca tataattgct gtgctaccat catggttttt cttgttcgaa
    33601 agtttaactt acgggcagct tcttgttgca ggctacttgt agtgaaagga ttcgcagggt
    33661 ttcttttgcg ctctttcttt tctacttttt taacttcgaa gtctgttcca tcaattctag
    33721 cagtgacttc ttttacactt tgtgcatctg gtaatttctt tttcttaccg tcgattcccc
    33781 agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt accgtcgatg ctccaatatt
    33841 cttctggaac aaattctcgg atctcttttt cacggtcaat aataattttt aaagcgacag
    33901 attggacacg tcctgcactt aaccctttct tgaccttacg ccagagaata ggactgatcg
    33961 aataaccaac gattctgtct aaggtacgac gagcttgctg tgcatctact aaatctacat
    34021 cgatcgttct tggttcctta aaagctgctt tgactgcgtc ttttgtgatt tcattgaaga
    34081 ccacccgatt tttttctttc agatccaacc caagtaggta cgctaaatgc caagcaatcg
    34141 cttctccttc tctatccggg tcacttgcca agtaaacttt ttgagctttt tttgctgcgg
    34201 ctttcaggct tttgatgaca tcgccttttc cgcgtataga aatataatgg ggttcataat
    34261 tgttttcaaa atcgataccc attttacttt tcggtaaatc tcttatatgg ccaacactgg
    34321 ccattacttt atagtttcgt ccaagatatt tctcaattgt cttggctttt gcaggtgatt
    34381 ctacaatcac taaatattta tacgccatta ctgaatggac tcctttccat tgtcgtgttc
    34441 ctactattaa aagaggattc atacaaatcg tagctaatca tatccattca gaaaacgttt
    34501 gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa attctgccaa gatatcttca
    34561 cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat gacatccggt agaatgttct
    34621 gttaaaatat ttcctggaac agaaaaaact gttcttccaa gatccaatgc ttgctgtgct
    34681 gttatcagag agccgctttt tttcttggct tcaaagacac atgttccttt agacaaagct
    34741 gcgataatcc gatttctcat tggaaaacaa tgctttttcg ggccggattg aggaggatat
    34801 tcactcaaaa gaagatgatc atttgcaatc acctcttgca agtcacgatt ttcagcagga
    34861 tagacttgct ctatccctgt tccaatgact gcgattgtcc gtccaccatg agagattgct
    34921 gcttggtgac cgtatgtatc tatccctttc gcgcagccac tgacgatcac atatccagct
    34981 tctatcactg gttttagtaa ataataaact gtttttttgg ctaaagcaga agtgtgtctt
    35041 ccaccaacaa cgcttaacat gttacaagaa agtaaagata gatcaccatt ataaaacagt
    35101 acaattggcg gataggtcaa gtgaagcagc tgagggggat atttgggatc aaagcaagtg
    35161 ataaacgact gtttcgattg gattttgccc cagttagcgt gaatttcctg ccaacttttg
    35221 cgaaacagct cccggtgttt ggttatttta cctatcttaa tgatgtcttc aaatccgatt
    35281 tctttataag gatactgcat catccagtta gcaagtgccc attttccttg actactgatt
    35341 ccttttgtat aagcaagttt caataaattt tcttctattt gatacatcat aaaaccgcct
    35401 ttcgtctctc tttctaataa gatacgtaaa agcggctgtt ttttattgta aatatttttt
    35461 tacaggtgca aaacttttac gatggattgg cgtaattcct tgtcgttcaa gtccttctaa
    35521 gtgttccttt gttccataac ctgcattatg cgagaaacca tatccaggat aaagcacgtc
    35581 atattctttc atcaattggt ctcgatatac tttagcaatg atacttgcag cagcaatcga
    35641 cacagatcgt gcatcccctt tgattatttt ttcctgctga ataggcaaat ccaaggtcat
    35701 cgcatcgatc aacaaatagc ttggtacaat ggacaatttt tctactgctt ggatcatcgc
    35761 taatttactt gcttcataaa tattcacatc atcgattgtc tgctgatcaa cgatcccgat
    35821 cccgattgcc agggcttttt cttgaacttg ttgataaaga agttctcgct ttttctctga
    35881 taattgtttg gagtcattca gccctaatat gatctcattt ttcggcaaga taactgcagc
    35941 tgccacgaca ggtccagcta atggtcctct tcctacttca tcaatgccgg caatccattg
    36001 gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt tcttcatatt tttccttaat
    36061 taatgcttcc tttttcaatc gtcgttcaaa agaagccagt gcttgctgca cgcctttgcg
    36121 tggatcattt cttaatgatt ctagtgtagg atcatctatt ctctgtatgc ttttcaatcg
    36181 ttctttgata ctttggatgg attcagtcat cacttttttg ctccatttct tcgcaacgat
    36241 ccagtgtgta tcgtccaagt tttccttgac gaatatcatg cacgactaat tcgctgccac
    36301 gatcatagtc ttctttatac ccacgttttg ctgtaatatc catcagtaat tccggtgcca
    36361 atttttcttc ttgctcgatt gataaaccat aacgatcgct gattcttcct gaataatatc
    36421 gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa atggacaagc tggtctttga
    36481 tcgcgcctgt cagcgctagt tttttgccga tttcttcatc ttcaaatttg ggccataaaa
    36541 tccctggtgt gtctaacaat tccaactcat tacctaaacg aagccactgc tgaccttttg
    36601 ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc tactagtcga ttcatcaacg
    36661 tagatttccc aacatttgga atacctaaga ccattgcccg aatagcgcga ggcttgatcc
    36721 cccgagcagc atctcttgcc cgtttttctt tcaatgcttc tttagccgca ggtactagac
    36781 ctttaattcc tttactttct tgtgccgtga tagctaatgc aagatggcct tgtttacgaa
    36841 aatattcttg ccacagcttt gtctgttgtg gatctgccag atccgcttta ttcaataaaa
    36901 caaggcgagg tttttgctga atgatctgat ctagcattgg attacgagaa gataaaggta
    36961 atcgtgcgtc taccaattca aatacgatgt ccacatattt cattttttca gaaacttctc
    37021 gtcgggcttt tgccatatgt cccggaaacc attggatggt cattgttctc tccattcctt
    37081 tcataccgga atattccccg gctttacttg tttttttctt tttcaatata aattcacttt
    37141 tttattataa agaaattcca tctattagtt aagtgaaagt gaaccagtcc tcattctatc
    37201 acatcttcac ttttttgatg atgcaaattt ttattttggt ttctatatat gaatctaaat
    37261 ttttgacagc gtttcctaaa taagagatga tagacgaaca aaacagaaag gagacgaaac
    37321 aaatgaatag tatggttttt gttaattttc ctgttgctga tgtcaatcgt tccgttgctt
    37381 tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc tactgaagaa gctggttcaa
    37441 tggtatggga tgacactttt tggataatgc ttctctctca tgatttttat aaaaaattta
    37501 taaaagataa agaaatagct gataataaaa aaacaagtgg tgctttgatt tcgtttagtg
    37561 ttgaaaatgc ggatgaagta aaaaaatttg ctcaaattgc aaaagaaaat ggtggtactt
    37621 tccacgaagt agatatggga atgcctgaag aagaaatgtt ttcattagaa gtccaagatc
    37681 tagacggaaa tacattagaa cccgtatgga tgaagatgta agcagataac taatctgacg
    37741 ttttttataa caaaaaaaca aaggcggatg atacgataag tattctccgc ctctattttt
    37801 tttattcgta caatctcttt tttacagttt ataaaagcag tcttctcacc ctttttttaa
    37861 ttgttagatg ccagtgtatg taaatcacca atgtgtcatg acacattggt gattttatgg
    37921 tatcctttta ttattaactg acaaaggaga gaatattttg aacactttga ttgcaaaaga
    37981 cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg acctctgctc ttttattagc
    38041 ccttgatgtc atcttataca agatagcgat tggtcctgct tattttcaaa tcacattcgg
    38101 atttttaagt atggcgctta tgggttatct atatggaccg atctgggctg gtttgcttga
    38161 aatgttgtcg aacactttaa atttcacgat atttggttca ggaacgtttc aagctgcttt
    38221 tcttatcacg gctttccttg gtggatttat taatggctta tttttatatc ataaaaagat
    38281 aaacctgcct tccgtcatcg ctgcccaatt attgattatg atcccagtca gcctttttat
    38341 caattcttgg ctgatgtctg ttttgtttgg tgcagatttc aaagtaattt ttctaggtag
    38401 attcatcagg aatatgatcc aaatccctat tcaaataatg gttgtttatt catttatcaa
    38461 aactttagag gctagaaaag tgtttaaacg agctgtttga ttttacgtac taaggtagta
    38521 aactgatttt ggattaaaca ataaagcgtg cagtctcaac agactgcacg ctttattgtt
    38581 tctcactgga tccattttat ttcatcaagt ggataataga caaatcttgc tttacctaga
    38641 atatcagttt tattgatcgt gccaaacgaa cgactatctt ttgaaactct tcgattatct
    38701 ccaagtacaa aataattatc ttccggaagc ttggattgtc ccgttagctc ttgtagtgta
    38761 aagtccgtcg tataggatgc atttgcatga tctgatttca ggtttttagt tagataaggt
    38821 tccttaacta ctttcccatt gatcttcaat tgatcatttt ggtagctgac attttctcct
    38881 ggaagaccga ttactcgttt gatataaatc gtgccatctg ctaattgaaa aacaacaata
    38941 tcaaatcgtc tgatctccga aaatttttcc atcacaacca tatcaccttg cttcaacacg
    39001 ttttccattg aattgccttc cactggtacc gggatgaata aaaatccccg caacatgaac
    39061 gcaaggaata cagaaacaag taagtatttg aatacaagcc agaagcgatt aatatagcgt
    39121 tgtttttttg tcaatctcta tcaatccatc cctctttttt tcattctttc tttattataa
    39181 gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa aataattctt tcaattaggc
    39241 tattggacaa aaaacttttt tgatcataaa gtacaaaact atctcttata tgtcatgatt
    39301 attttctaaa tatttttccc atgcttcgtc aaagatatcc atggacggca aatagtcgac
    39361 attcagttcc agataattag aaatttcttc gtagctagag ctgtgcttag gaaattggat
    39421 gtctttaccg gcttctgtag cgaaaacaga taaagcatca tgtgtgccac cgcctcgcag
    39481 tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt tcctccaatt ttcttcgata
    39541 aaggcagccc ggcgtgcatg gtttctttta aaattttcag gatgcttttt ataatactct
    39601 tgatggtctt cttctgctag ataaaatggt tgggctggtt cgatttttgt aacgattggc
    39661 tgttcgaaac gtccactttc agctaactgt ttcttacttt tttcagcaat gatccgctgt
    39721 tcttctgtca aataaaagat caccgggcga taattgtcac cgcgatcctc gaattgtccg
    39781 aaagcgtcag ttggatcagt ttgctgccag taaagttcta ctaattgttc atatgaaatc
    39841 aatgttggat catagataat ctccactgct tctgtatgtc cagtcgtttg gctttttact
    39901 tgttcataag tcggatctgg tacatgccct ccagtatatc ctgacatcac tgtatggata
    39961 ccaggaagcg tgtcgaaagg ctggatcata caccagaagc agcctcctgc aaatattgct
    40021 cgttccatta tgattccccc tctaatacta ctcatccgat gattttttat tttcttttgt
    40081 cttgctttca gtactgctgg tgctagtttt gttggtattt gagttgtttg aagcatcaga
    40141 ggtatctttt ggcaaatata gactaaaccg aatgtcatca tttactaagt cgattttatc
    40201 tgctttaata tacatcccgt tttgcattcg gaattgatcc aacctaagca tgatagtcag
    40261 atcatctgta ttgacttcga tccaatctgg taatttatag tttctcttga tcatattcat
    40321 cacatctttg attggcaaac tcaatgttcc gatagaaaga cttctagctt ttagttggac
    40381 atttccgttt tccatcacat acggatcaaa atataaatag aaatcgatag gaaaacctag
    40441 tacttcgaaa gtaccattga gtagtgcttc attttccaaa taaaatttat attcaatatc
    40501 actatctttt tgatactcac taaggaaaaa gctgatgatt tgattgactt tttctttatt
    40561 tgaattgatc gttaagacag gatctccttg tctttccgta agagctggta tttctgggag
    40621 ttctggttcc cgattagcaa aaatacggct gcctaaaaat acggtcgaac caagtactac
    40681 cccaagaagg ataaggaagg cgaatttcca gtaatttgtt ttctttgttt tgtttgaaga
    40741 tacgtcagat cttgttttca tgtttattga tcatcctcct tctctaacca ttggttttgt
    40801 gtctcgatca gctgatctct cactgcattt gccatcaatt gataacctaa attatttgga
    40861 tggaaatgat cttcttcata gagcacatta tttttgatgc catttagatt ttctgtcgtg
    40921 ctagttgttt gatctgttcc ttcttctgtg acacctactt cctgattcaa tccttgataa
    40981 agcaaatcat tgattgggat aaagtagcag ttcggcattt tttttgtcac cgtttctgtt
    41041 cctttgttcc aattgttaac aattgtctgc atgtcagtga tctcaggaaa gttcaaataa
    41101 aatggattgt agatgcctaa aatgtaaata ggtgcggttt tattgaggga tcgaaattct
    41161 tccaatagtt ctgtgacatt ttcctgatag tttttcagtg gctttttaaa ggaattgacc
    41221 gttaacccga ataaattatt ttggataact ttcatcaaat cattaccgcc aacagtcagt
    41281 gtgataaaat cagctgtttt gatattgttt cgaatttctt cttttgtttt tactcgcttt
    41341 aagatctggt cacttcgttc accatttaca ccatagtttt ctgtttcaac cgcggtcaaa
    41401 ttataacggt cttttagatc atttgcgaca aggggaacga atcctccttg cttggtttga
    41461 tctcctactc cttcagtcag cgaatctccg atagctatgt agtgaagttt ttctttttta
    41521 tttggatctt ctctcacagt agttactggt ttcattagtg ggccagcttt cggtatagaa
    41581 gcagctaaac ctgcatagat gatgatcgca attaataatg ggaacaaaac gaggatcaaa
    41641 tgtcgtttcg ttaatttcat tttttcacct cttgtattcg attggttcta atgttatcaa
    41701 aaattttatc aaaaaggtag tctttatgaa aaaagaaaac aattattttc ctaattttat
    41761 aaagaaaaag agaataagct gcaagccttt caggctgagg gcttatcctc ttttcgacta
    41821 atctacataa tacatgattg caaacgcgtc tttaccagta tgcgtagcga taaccggatt
    41881 cgtatgcagt acagggatat gcatgtccgg gaataattct tgcaaaccct ctttgaattc
    41941 ttgcgccaat tcttttgcat ccgcatgtga aataccgatt tgtcgcacat ttggcaattc
    42001 tttcagttca tttttcaaat tattaaacca tttatcgaac gttttctttc ctcgcccctt
    42061 agttacaggg attaattcag aatgggcgaa attcatcacc acgcgtatgt tcaaaacact
    42121 agacagcaaa ccagttgttc ggctgattct tccacctttt accaagttat ctagtgtgga
    42181 tattccgatg tataattttg tattgtcttg tacttttttg acttcttcaa ggattttctc
    42241 tactgtttta ccttcttttg ctaattcagc tgctcgaatt acctgaaaag ataatccttg
    42301 gtcagtggta tcactgtcga taaccgttac tttcgttttt gttagattac tggcttgtct
    42361 tgctgcttct actgttccac tcaagccttt tgtcatatgg atcgaaatca cttcactgcc
    42421 gtcttctcct agctcatcat acatcgagat gaattctcca attggtggtt ggcttgtttt
    42481 gggcagattt tttgctttcg ccatcatttc cataaatctt tcgccgtcta gttggtcatc
    42541 atcgggatat actgttccgt cgatcatgat ggatagcggc atgacacgaa tgcctaaagc
    42601 atcgcgtaca tttttttcca tcgtacatga agaatctgtt acgattttta catttgtcat
    42661 tattttctct ctctctttct ttaacaagat gccttattca tgttaaacta catgtaagac
    42721 aatttctatt tttagtataa cagacaaata atgatttttc attaattaca cctagtaaag
    42781 gagccgtttt catggaaaaa acgaaattta atcgaacgta tgaaatatta aatgaagtgt
    42841 ttaatgcagt cacccatgga attgggactg gattgagtat cgcgggacta gtggttcttt
    42901 tagtaaaagg tgcccacttg cattcctccc tccatattgt ctcttatgct atctatggat
    42961 cgatgatgat tcttttattt ttaacgtcta ctttgtttca ttcattgatt tttacaaaag
    43021 caaaaaaggt gttccaagta ttcgatcata gttcgatttt tcttttgatt gcaggaagtt
    43081 atactccttt ttgtttgctg agtatccgag gatggctagg ttggacttta tttgtattaa
    43141 tctggttgtt tgcgattctt ggcattatat ataaagcgtt gacgttgcat aaacgggaga
    43201 gagtatcaaa agtatctacg atcatctata tttttatggg gtggctctgt gtcgtggctg
    43261 ctgttcctct ttatcactct cttggaatga ctggtgttat tttgatggta gcaggcgggc
    43321 tttcttacac gataggcgca ttcttttata gcttgaattc tatccgctat atgcatgttg
    43381 tttggcattt gtttgttatg ttaggtgcag gttttatgta cttctctgtt ttattcttta
    43441 cttaataaaa aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat
    43501 agctttttta tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat
    43561 acgtttcaaa ttgatgcgga taacgatttt tttcatccat ttctccttga ctagtattga
    43621 tcagtgacca atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat
    43681 ggatgactgt acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc
    43741 ctccagcgat gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac
    43801 tatgcatgac atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct
    43861 gacgattcgg caacggacgg ctacccatcc cttcgaatgt ttttcgtccc atcactagtg
    43921 tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac
    43981 ggtcttcttt tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct
    44041 catcctttct gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct
    44101 tctaattgga tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact
    44161 aaagtcggaa atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg
    44221 atataaagat gagcatctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca
    44281 tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac
    44341 acatccccac tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga
    44401 aacatcgtat gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa
    44461 acgatcaatc ttctagaatc aggagtcttt ttgattgctt cgatcacttg tttgatttga
    44521 tcgataaagc caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca
    44581 agatcgccgt atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg
    44641 ctctgctttt tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca
    44701 gtcatatctg gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc
    44761 caaatatggt ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa
    44821 agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct
    44881 ttttgcagat caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg
    44941 tcttctttac gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc
    45001 atcctttttc ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt
    45061 cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat
    45121 tatctccttg atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa
    45181 tctcatcttc aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag
    45241 caggttcctt gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt
    45301 gcttcagtaa ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga
    45361 atactaataa cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca
    45421 cagcacctgg aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat
    45481 cattggtcgg ttcatccaat aataaaacat taggttgctg aatcagcagt ttcaacaaaa
    45541 ataaccggcg tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg
    45601 gaaataaaaa tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg
    45661 cttcttcagc agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat
    45721 tttcttgtgt ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca
    45781 ggggaattct gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag
    45841 taatacctag tctttcccga gattgaatca ataaatctaa atgttctagc agtgtttggt
    45901 catttatacg gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg
    45961 ctaggttcag ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt
    46021 cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc
    46081 aagctaattc ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat
    46141 cacgttctgc tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc
    46201 caaatgaaag ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa
    46261 ccatcaatag tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat
    46321 aatctaaatg gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt
    46381 gcgccagact aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt
    46441 cactgattcc taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt
    46501 ctttattcat ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt
    46561 tttcccctag ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt
    46621 ttccttgaaa gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa
    46681 gataaccaat acggtaatca gttggataaa acactgtttg ccgatcaccg tcgctgctat
    46741 cgataccggc aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc
    46801 cgatacggtc tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat
    46861 aagttttttt gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat
    46921 attgtatcat aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt
    46981 tctcaaaaaa agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa
    47041 cttgaaaatt tttacctttt tattttattg gactcgtttt ttttgacttt gctcgaaacg
    47101 caaatcggaa agaaaagtta tcagatggtt tgttcatcgt ataagattct aacaaattga
    47161 tattcctgcg atagctgttc gcagaaaacg tcgtgatttt ttcagcaact tcgtattcat
    47221 caatgatttt tctttcaaga taataacctc ttagcatttt tttcgtaaac agactatctt
    47281 gctggatcat ccacgaagaa atcaaattcc ccgagttcat ttttattcct tcaagtttgc
    47341 gttgctggat aaaaaattga accatcgttt catcataaat atcagtcaag ttatctaaac
    47401 tttcaataat caactgagta tttttttgat atgtctctct gatttcttgg atatcttcat
    47461 cactaataaa tgtctgacga tcagcatatc ttctttgcca aaacattttc ggatggagaa
    47521 cgattcgcat taacctacgg acaacaatga accaaaagcc gataaaagac aaaatctggc
    47581 ttgtgataga atgttcgaat cgagaaagaa aacgggagta aaaacgataa ccattagaag
    47641 aaagcttgcc cgaacgataa ctttcatcca atccatcccg ttcgatcgac aaaatcagtg
    47701 cttgtatttc ttgtacttct tgcttttcat catcggttaa atcttccaag tacagatcac
    47761 gtatccgttc ttgataattt tctatcactg cttcagtggc taaaaattct tttgtattac
    47821 gatcaatttc ctttatttct tttcgcaaaa cttcaacgac ctcttctaaa atcatcaaag
    47881 cattcaaatc cgtgcttttc gcttcctctg tttccgtcag cataggcaag acaatgatcc
    47941 ctatgaccaa agtcactaag atgacacccg ccgtcagaaa caaaagcaaa gaccgctggt
    48001 aaaacatcat attattgatt gaaaatggca aaataaaaat cgtagccaaa ctgactgttc
    48061 cctttacacc gccaaatgtt aagatcaaga tctcgttcaa ctgttttttg aactttttac
    48121 tgccatcttt gaatacataa aaaagactga tagaaatgaa tctgatcaca aaaagcatga
    48181 cagagattaa tacgataatc gctaataaaa gaccgtttgg atataaaccg tctccccata
    48241 ctggcgagaa cacttgcgtc aattcgattc ctaaaaatat aaatactaat gcattcagtg
    48301 taaaagcaat agttgtccat gtactgtggg acaagctcga caattctgca tcaaacacag
    48361 agattttcct aaaaccagaa gcttggagaa tacctgctgc cactgcagca atgattccag
    48421 atacatcaaa tgcctctgcc aatacatagg ctagaaaagg aagcaaaagt tcgatcagca
    48481 gataccctgt gacatcttgt gctgatgctt tttcgatcaa atgaattatt ttttgtttga
    48541 accagacaag caaaaagccg atcaaagctc ccccaatact agaaactact agactcatcc
    48601 ccgcgtttac tgctgaaaaa gatccggtga tcagcgcacc aagagcaaac tggaaggcag
    48661 taacaccaga tgcatcattc aaaagacctt ctccttccaa aatatgcatc gcctttttag
    48721 ggatattcac tcgtccagaa agagaactga ctgctaccgc atctgtcgga cctaacgctg
    48781 ctccaaaagc aaagcaggca gcaagtggga cacttggcaa caggaatgat aacgtcgcac
    48841 ctaccccgac aagggtcaaa atgactcctc caaatgccaa gaacaaaatt gtcccgaaat
    48901 ttttgagaat cgaagaaata tctgcttttt ctccttctct aaataacaat ggtgcaataa
    48961 tcataactaa gaacaattca ggttcaaaat cgattgattg tccccactct gtcagaccta
    49021 ataccactcc aaggaaaatt tggatcagtg gagctggaat cattggcaaa atgcggctag
    49081 ctatgttcga taaggtgatc gttactgcaa atacaataac taactcagca aattccatcg
    49141 tccatcccct ttatttttta ttttgtgtaa gcatttttaa aaattgttct tttttcttta
    49201 aaattttggt ttcgactact tcaacctgtg tttggttccc ctctaattca cactgtacac
    49261 gttttgactc caaacgaagc agatcatgtt tcagacgttc caactgacga tacatcaata
    49321 tatttctttt acgcaactta caaagtattt gacggcggtc atccatcgaa agggcgccat
    49381 agcgatcatg caacagtttt ttttgttgtt caaaactctc tctcattttc cagatctcct
    49441 atacacaaac ttccatcatt atagcaaagt tctttttgga tgaaaaatat ttcattgttt
    49501 catcgtttat tgctagttag aaatttccat aaatttttca acgcctcatt ctattccaaa
    49561 tagtatcaga tatcaagtaa aacttaagaa agataaaagt ttctgacaaa aggatgttta
    49621 ctttcccatt cctgcctttc ttgttctttt atcacaacaa aaaaatgggg ctgcgacaaa
    49681 aatcttgtca cagtccctta ttctgaataa acggtgtgga agagacagcc tatcagtagt
    49741 aataagacac atctctttca cagcctcatt tttgcgtaaa aatcgattta ttcgaaaaat
    49801 tccccgtata catcttctac acgttgatct ggattgatca cgatatacaa gttacctgtt
    49861 ttttcgctga tttttgttga ttgatattga ttgtctaatc cttctgttcc tttatctaga
    49921 tatgggaagt acacaagatc aggtgtgcca tttcggtaaa ggatttccat tcgctcgata
    49981 tcttcgtatt tcaatgcgcg attaaacatg cccatttcca gtccgcctag gttgatatca
    50041 tgtgtcgcaa ctgcgtcccc ttcatggaag atttcgattt taaatccagc acacggatgg
    50101 atttcaacaa attcactgcc attgattcgt ccaaaacttg ttgtgatttg tttgatccaa
    50161 agatcgccga tatgacggcg gttgatcgtc caggtttctc catttctaaa ataaaatttt
    50221 agcgctgtca tttctttact cattaaaaag actcctttat gatgtaattt attcttgtac
    50281 tacctcagtt tagcacaaat ttatgtaagt ggtcacatct tatgtttttt cttctaatga
    50341 cgtatctgcc cactttaagt agccaacaaa gctgagcaga tacagtctag tgttttttaa
    50401 atcaattttt ctttcgataa aaaatccaaa acctgttttt tatcattttt tattctttgt
    50461 tctacgacgg ctttttcagc taattgcaaa gcttcactaa cccacttacc aggttttttt
    50521 ccagtttctt ctagcaagat tttaccatct actgctaatt cgcttaatga cttgataggt
    50581 aaacgatcat aggattctcc tacggcatcc atctgaggtg tttcgtcaaa atagatcatc
    50641 aattcttcta cttgcagtgc atgttttttg cctaagaagt acaattcgaa tggttccaaa
    50701 gctccagttt ctcgttgtcg tagtccttgg acaagtgctt gaacgtcttt aatcatttgg
    50761 ttagaacact tccacgattt caaaaaggaa cgaatctcat tttcttttag atccagttgg
    50821 ctgatcaata atgcccatgc ttggctctct tctttcaatg ggacggatgg taattcagca
    50881 aaacgtaaaa gtgcttcgcc ttcttctttc aaaccgggac agcaaatgta gcactctgtt
    50941 tcaataaatg catggattcc cgcggcccgt tcatttccca agagcatctt gatgaattcc
    51001 acatttatac gttctacaga tattttacct aatagctcgt ggttttcttt gatggcgtag
    51061 aaggtttctt cctctaattt gaaccctagt tgactgacaa aacgcaaccc gcgcatcatt
    51121 cgtaaggcat cttcatggaa ccgctcatga ggattgccta cagctcgcaa gatctgttct
    51181 tctagatctt gcagaccatc gaacaaatcg atgatttctc catcttcttt caaagcaaac
    51241 gcattgatcg taaaatctct tcttttcaaa tcttctgcca acgaacgaac gaattcgaca
    51301 tgatctggtc tacggtagtc ctgataagtt gattccgtcc gaaatgtggt gatctcatat
    51361 tgttcatcat tttctaatac caacacagtt ccatgttcga tcccaatatc tatcgttttt
    51421 ggaaaaagct gtttgatttc ttctggaaat gcgcttgttg cgatatccac atcatggatt
    51481 ggctggttca gcaagatatc acgaacactt ccacctacaa aataagcttc atatcctgca
    51541 ttctctaatt ttttcagaac tggaattgcc ttttggtatt ccagaggtaa ttgttttaat
    51601 ttcatttttc caacctttcc tgcgtgtttc ttgtcctgag ataaagactc agttgcatgg
    51661 tgactgtttg acaagtccgt ttcagagtct tcaaggccag gaccacttca tcttgtttac
    51721 tcgtccttac gctcaaagcg attatggtct ctttgattga atttttccat gtttttttca
    51781 aaaacttcag ttaagtcgat attcaatgaa ttagccatga tcatcgtaac gaataaaaca
    51841 tcaccaagtt cttctgctac cgagttaact ggttcagaag cctttttgct tttttctcca
    51901 taagtgtggt tgacttctct tgccaattca cctacttctt cagttaatcg agccatctga
    51961 gctaatggag agaaataccc caccttgaat tgctggatat attcatccac ttcctgctgc
    52021 atactgtata aagaacgctt tttatccatc tttttccctc caaaactatc ttaccaaaac
    52081 tttaacgcaa atcctagata agagcaattg agaaaatcga atttccatgc tacatttagg
    52141 aaggaatttc taaatggaaa ttctgtgaaa atacataatt gaaagctgga attcatatgg
    52201 tcaaacgaaa aacagtaaaa gaagttcttt ttatcattat aggaacaagt atttatgcat
    52261 ttggtcttgt ttacttgaac atcgctaatc atttagcaga agggggagtc tcagggatca
    52321 ccttgatctt aagagcttta tttggtattg atccagctta tacgacttta ttaatcaaca
    52381 tcccattgat tttactagga ggaaagatcc tcggtaaacg ttccttggcc tatactgttc
    52441 taggtacact ttctttgtca ttctttttat ggatctggca aagggttcca ctagaaatca
    52501 atttacagca tgatctactg atcgtttctc tacttgcggg tttagttgca ggtgtaggaa
    52561 gcgggatcgt ctaccgcatg ggtggaacaa caggcggcag tgatattatc gctcggattt
    52621 tagaacaaaa ttacggcatc actatggggc gttccctatt ggcatttgat atcatcgtct
    52681 tacttgcttc attaacctat atcgacttga aacgtatgat gtatacgttg attgtttcgt
    52741 atgttttcag tcgtgtgatt gattcgatct tagatggcgg atattctgca aaaggtctac
    52801 ttgtcgtctc caacaaaagc gaagagattg caccactact gatgaccggt ctgcaaagag
    52861 gtgtgacttt cctttctgga gaaggcgcat tctctggtaa ttcaaaaaaa atcatctata
    52921 tggtcgttag ctctagtgaa ttaactgaag ctaagaggat cgtccacgag attgacgaaa
    52981 aagctttcct ttcaatcatc aatgtgcatg aagtggaagg gcaaggcttt acttatttga
    53041 agccacagac aagattactg aaaaaactat aagaaaaaag acaaagatag gctatgagga
    53101 aagattccac atccagctga cctttgtctt ttttagtact atactatttt taactaattg
    53161 ttatgttcac ttacccggtt acttttcttt tgtgtctgca aaaggatctt tcggttaact
    53221 tcatcagata atttcaaaaa agcatcccta tctccatctt ctagtgcttg atcgatttga
    53281 gcataaagca tttttagttt tgcctcttct tctttttctt gaaaatatcg ttcaattaat
    53341 tcagtatctt cttcactgac tgaagaatcc caagaagaaa atggattgtc ttctaagata
    53401 gatagatatt ctcttgtctg ccaagcattt tcgaatatac attccacata taaaggcttt
    53461 ttccaattca aacggatttc atgaaaaatc tggtctgtgt ccgtaaattc ttttccctgc
    53521 aaaaacaatt tcatgggttc cccttcaaca gatagatcac tgatctgcag tcctcgatcc
    53581 gcaaaattcg ccccttcaac aaagtggaca ttgcttaaaa tcgcttcatg attggcaaga
    53641 taattcaaga tccaaaaaac ttctcttcgg ctaaaagata catgattgac catccacgtt
    53701 aaaaaattct tcttttcccg tacatcaatt agcatttttc tcacctctct gttaggtcat
    53761 ctaataatga cagtgcctcc atatctcctg gttcaagtgt taaataataa gtcaataatt
    53821 cttgcgctct tttcagctgc ccttcttctc ttaagaaaaa ggcatactct ttcaagaaat
    53881 ctggttcatg tgatagttca tgataagcct gttcatagtg tacagctgca acggtaaagt
    53941 cttctaattc attataagca tgggctaggt tccattctgc ataaggatta gcagtttctt
    54001 ccatctgatt gatcacttgt atcacatctt catatctttc atcatccaag taaagattac
    54061 ttaaagtaag taatgtttca tcttgtttgt ctcctacttc taaagctttc agtaaaagtt
    54121 cttctgctcg ctgtctatca tgcagacgga aagcattttc cgaggccagg tgataaagtt
    54181 cgacttggta aggattttct ttgattcctt cttctaacac tgtttgtgct tcttcaatca
    54241 tttcttcttc ttgaagagct tgtcctagat atagatataa cgattgataa tgcggattaa
    54301 cttctcgaag ttcttccaga tagttgattg cctgctgatt ttctttcaat tgaagattcg
    54361 taaaagctag ctggaacagt cgatcatctg tcctttcttc tttcagtgct tcttctaaag
    54421 gtacgatagc tgcttcgaat tctccttgca tacttaagga ttgtcctaaa cgttcctgca
    54481 tcgagattcc tgaaatttca tttatcccgc ttgccaacaa tgtttcataa accactgctg
    54541 cctctttgaa acgatcaact gaaaaataca attcagctag cgcaaactgg atcaatggtt
    54601 catcaggaag caacccagct gcttctttca attttgcttc actcacttca gggattccga
    54661 taacttggta caaatcagct atcgccaata agctttgcgg atagtaatca ctttgcttat
    54721 ccactttttc caggtattca aaagctaagt cgatctcatt atcttcgatt gcaatttctg
    54781 ctaacgggat gttcagtccg tcattttctg ggtattggtt gatcagttgt tcaaaaatct
    54841 gttttgcttc gatcaaaaag ccaatagcca gcaattcttc gcctagttca gctaatatat
    54901 ctgctgggtc ttcttttaat gctttttcta aaaagatttg tgcttgtgac aagtcttcat
    54961 ctgataaagc ttgcaacatt ttttcgctat ttgtattcat ttcaattccc tcttatctca
    55021 ctgacgattt gttgaaatac cgtcattgtt ctcaataatt ctttttcgtt tggatgttgg
    55081 tatccgtcta ttatacctat tttttctaaa acaagtattt ttttgctttt ttctgccaaa
    55141 aaaagaactt tttccaaata ctccatcttt gaaatctgtt ccagccagtg gattggatag
    55201 ccaagcaagt ccagccacac aaaaaagttt tttagttgga aatcaaaagg atatgcttgg
    55261 agattccaca cgaaatgaaa catcattcct aaaaattttt tcatactagc agaaagtaga
    55321 tggccatttt cggtcagata aaaagcttgt tcaaacagtt taccataatt tgacaggttt
    55381 tctgcctcta attgataaaa atagatcatt tcttcgatca tcccggcaaa cggccgagtc
    55441 gttaattgct ttttatttgg atagttttta tacagatttt gcaaaaaacg atgatcacaa
    55501 atcattccgc aacagataaa agtcaaaaag tcaatctgct ttccgtccac ctgccgctcg
    55561 ctgattgttt gatcataaac gatgcgttct ggcagattga tggtttgcag cactacacga
    55621 tttggtagct gcaaaatcgt ccgctgcggt gttaatgctc ggcttagaga acggatcgat
    55681 acagggatca tccatagtga tgaatcaagg gttgtatgtt tttgaaaaaa acctgccaga
    55741 tccatgatcc cttcatttcc aaatccgatg ataaacatgg attgattttc gggatagcgt
    55801 ttgacgaaat caagcaggtt ctttaattct gttaaatgat tgcattgagt gttggcacaa
    55861 atataccagt cattgtttgc ttgatttgca aataacgggt tgatcttatc tgcaaacaaa
    55921 tcataatagc gctgattcgt taaaaataag attggctgat caatggaaag ttctcttagc
    55981 tgactgacta gcgtttctcc gtaaaacact ttcgtcaacg catcttctac ttgataatca
    56041 agttccatac gcatccctcc tcttcttatt tttattttac cattagtaag caaattctag
    56101 aaaagataag agcaaagctc acctcataac ttgcaaataa ataaaaatta aataaaatac
    56161 atgtgttttc tatgtatgac atacggattc ggaatctctg tctcttattt attccttttc
    56221 tgctaggaaa taaaaagaaa agcacttgta gccctagagc cacaagcgct ttttcaactg
    56281 tcttatttaa cagcgtcttt taatgcttta cctggtttga atgcaggtac tttgctagct
    56341 ggaatttcga tttcttcacc agtttgtggg ttacgacctt tgcgagctgc gcgttcgcga
    56401 acttcaaagt taccaaaacc gattaattga actttttcgc cgttagctaa agcatcttga
    56461 atagttgaaa atacagcatc tactgctgca gttgcatctt ttttagttaa atcagtagct
    56521 gaagcaactt tttcgattaa ttctgctttg tttgccatgg atagattcac ctcctccaca
    56581 agagatatga tagaaatatc atttagttaa aactttctga gtggtccaga agtttaaaaa
    56641 gaaatattga ttaacttcaa tattctgtta tcacgatatc atatgaaccg cttaatagca
    56701 aggtttttac caattttcac atgctttttt tagtcataga aacgtaacaa acgaaactgt
    56761 ctttttcttt tgaaaaaaaa acgtcgatat ggtaaaatgc ctattttctc ctacgaggga
    56821 taattcggat ggacgtccct tcaaaagtaa atgcttttct gatttgattt tctaagaaac
    56881 gttcataaga aaaatgcatc aattcctctt cattgacaaa aacgacaaat gtaggtggtt
    56941 tgacagcgac ttgtgtagca taaaaaatct tcaggcgttt tcctttatct gttggtgttg
    57001 gattgattgc tacagcatcc ataatgatat cattcaacac agccgaagag atacgaagat
    57061 tctggttcat acttacttgt tcgatcattt caggcagacg ttccaaccgt tgtttagtca
    57121 ctgcagaaac aaaaacgatt ggagcgtaat caagataccg gaattcatca cgaatctctt
    57181 gttcgaaatc acgcattgta ttggtttctt tctttacaag atcccattta ttcacgacga
    57241 taataattcc acgaccagct tcatgggcgt aacccgctac tcgcttgtct tgttcccgaa
    57301 tcccttcttc agcattcagt accatcaaaa cgatatcaga acggtcaatg gcacgcattg
    57361 cacgcatcac gctgtatttt tcagtatttt cgtatacttt tccgcgttta cgcataccag
    57421 ctgtatctat cattaagaat ttctgaccac tttcagaaac gaagtgggtg tcgattgcgt
    57481 ctcgggtcgt tccttcaata tccgaaacaa ttacacgttc ttctcctaaa atcgcattga
    57541 tcaaagagga tttaccaaca tttgggcgac caatcaaact aaacttaatc gtgtcgtcgt
    57601 cctcttcttc agtctcttca gagaaatact tcactgcttc atctaaaata tcaccaatcc
    57661 ctagtccatg gcttcctgaa acaggataag gatcgcctag ccctaaggaa taaaattcat
    57721 agatatcgtt tctcatttct ggattatcta ctttattgac tgctaaaatc actggtttat
    57781 tacttctgta taaaattttc gcaaccatct catctgcgtc tgtcacgcct tcgcgtccgc
    57841 tgactacaca aatgattaca tctgcttctt caatcgcgat ttctgcttga tgcttgatct
    57901 gatccataaa aggttcatcg ccaagatcaa ttccgcctgt gtcgatcacg ctgaattctc
    57961 gacctaacca ttcacctttt gcataaatac gatcacgagt tactccagga gtatcttcga
    58021 caatcgagat acgctcacca gcgatgcggt taaaaatcgt agacttaccg acgttcgggc
    58081 ggccaacgat tgcaattgtt ggatttgcca tattatttcc tcctcacaag tttccaatct
    58141 cacttagttt aacgaatgca ttgccaactt tcaagcatag attgtcaatt aaacgaagta
    58201 tttttattcc ttattgaaat actaatcgct tttcaaaaga aaaactgaga caaaagcagc
    58261 tgctttcatc tcagtcacag atacaaaatc aattattttt cagaatcatc ggttgctttt
    58321 tcttctactt cttctttcaa cgcatcacct aaaatgtctc ccatagtaaa tccagtattt
    58381 tcttctggta attcataaga ctcaacattt tttggttctt cttctgaagc tggtttttct
    58441 tctaaagctt tgatactcaa tgcaatacga tgttcttctg gatggatctc taatactttc
    58501 actttcactt cgtcaccttc atgaagcact tcatgtggtg tcgcaatatg tttatgtgaa
    58561 atttgagaga tatggactag cccttctact ccagggaaca cttcaacaaa ggcaccgaag
    58621 cttgtcaaac gtttaactgt tccgtctaat actgtgccaa ctggagcttt tgtatcgatt
    58681 tctgaccaag gtccaggctg tgtgtctttg attgacaagg aaacacgttc ttgttcagga
    58741 ttgatagaca acactttgac atttacatca tcattgacag ccaatacatc acttggtttt
    58801 gctacatgac tatgtgaaat ctctgagaca tggactagac cgtcaattcc gcctaggtcg
    58861 acaaatgcgc caaaatcagt aagacgtgca acacgtccac tgagaacatc tccctcatgg
    58921 atagttgaaa gaatttcttt tttcttttct tctctttcgt tttccactac cgctttatgc
    58981 gataagatca aacgattttc tgatggttcg atttcgatga ttttaaagct caatgttttg
    59041 cctttatagt ctgaaaagtc tgcaacaaaa tgatcttcca ccattgatgc agggacaaaa
    59101 ccacgtacac cgacgtcaac gactaaaccg cctttgacaa catttgtaac aggtgcttcg
    59161 atgaccttgc cctcttggaa atctctttcg atatcttccc aaactttttt agcatctaaa
    59221 cggcgttttg aaagtagata gctgccgttt tctttatctt tgccgatcgt actgataacg
    59281 actaaatcta atacatcacc gacttttact acttcgctga tatcttctac cggcaaagtt
    59341 gataattctt ttgccggaac gactccttca actccagccc cttcgattcc gacaatcact
    59401 tgtttgtctt caatgactaa aacttcgccc ttaacaacat cgccaacttt tacttcttgt
    59461 acgcttttca ttgcgtcttc cattgactgg ttgttctctt gattttgatc aaattctgtc
    59521 ataacacttg tcctcctaac caacattctg ccgtaagtac ggtactgttt ttagtttaat
    59581 tgattattca aaaaaaataa agcgatttcg ttcgttttcc ttgaaaaaac aattgttttt
    59641 catttttttt cttaatttaa aaaagaagat aacctttttt caaaacaaca gcttttattg
    59701 ctgcaacgac ttctggaatc gatttgccag tcgtatcgat acgaacagca tcctctgctt
    59761 gcttcagcgg agagacgtcc cttgttgaat cataatggtc tctttcctgt atctctttcg
    59821 tcagtgtttc aagatcagtc ggaattcctt tttcttggtt ctctttgaaa cgtctttctg
    59881 cacgctcact tacgcttgct actaaaaaga ttttcacttc tgcattcggt aatacagctg
    59941 tcccgatgtc tcttccgtcc atgacaacgc caccgcgttc ccctattttt cgttggacag
    60001 caactaattc ttctctgact tttccatgtg cagagacttc agaaacagct tttgtgacat
    60061 ctggttgacg gatttctaaa gtcacatctt ttgatgctat gaatacatgc tgtccatctt
    60121 cttgttgcga aaaagagata ggatactttt tgatcagcga aactagccct tcttcatctg
    60181 aaaaagatac attgttcgta atagctaaat aagttactgc gcgatacatg gcaccggtat
    60241 ctgtataaat ataattcaat tcttttgcta atatcttggc aacggtactt ttcccagatg
    60301 aagcaggccc gtcgattgca atactgattt gtgtcatgat ataactcctt tttctcagaa
    60361 aaaaagccgc aaagcgcggc tattattatt taattcttaa ttcttgacca gggtaaagca
    60421 taaagttgtt tggatctaag ccattcaatt caaacaattg atcagtggtg atccctgcac
    60481 gctcagccac ttgttttgga ccttcgcctg cttgaacagt cgttgttgaa ccggctgcag
    60541 ctgatgaaga aggtgttgtt tctgctgcat tgctgcttga atcccctgct tgataagttg
    60601 cctcacttga ggaagtcggt gttgtctctg ctgcaatact gctttctgac gttacacctg
    60661 tatcttgaga cacagtagat tcttccgcga ttgtactaga ttctactgtt gaagtctcag
    60721 ttgactcctc ttttgttgta cttgtagaac tttctactac tgatgtcgaa gtttcagaag
    60781 aagttgctgc tgttgcattg ttgcttgatc catttctgat ccagaaataa gcacctgctg
    60841 gaatagcgat acataagacc agtaaaatca aaaatagcga aagaaataat gtatttcctt
    60901 tcttttgttg tctctgagaa cttcttgatg aaacttcatc atctgtatcg tagattggtt
    60961 gttcccatgg ttcttgtgtt tcgttatttt ctgtagattg atgtctatct tttctgctca
    61021 ctgttttacc tcctaaactc tatcgtgtgt attataccat aggaaaaagt gtcttgtcct
    61081 aaaaaatcaa tgttccatgt tttttttaaa taaatttagt aaaatcgttc cccaatgaag
    61141 ttgttcagtc ttagaaaaaa gcttcctctt tggcagaagt tctgcttttg ctccatcaat
    61201 atcacaacaa ttctgcggct tttcggagag tttttcgcca aaataagcta agatgaattt
    61261 tctgcggcag ttttcttcat taatatagcg aagcatctgc tgcaggcgga attctttttc
    61321 tttttcctga cgtttgagcc gttccaccca attttctggt ttttcagact gctggatcag
    61381 ctccatccat tttttttgca gctcatcaaa gggagcttgc tctgtggcat attccaaata
    61441 aagctcaaaa ctttgtcgct gttctctaga aagttgatta aagaaataat ggatacgttc
    61501 atccccactt tgataaaaaa gaattgctgc actttcttcc tgatctctgc ctgctcgacc
    61561 gatttcttgt acatagtttt ctagactgtc tggcaaatca taatggacca cataacggat
    61621 atcagattta tcgatcccca taccaaatgc attcgtagca atgagaaact gcagctgatt
    61681 ttttacaaac tgctgttgaa gttgtcttct ttgagctgca tcaagaccac cgtgatagta
    61741 tccaactgta aaacgttccc tgaataaatg atacaatcgt tcgacttctt tttttgttgc
    61801 acaataaatg attgccgctc cttgtgctct ttccatgaag tgttcaagtt cttgttcttt
    61861 ttgctgcgtt ttccgaacaa aaagcgagat attttgccga ttgactgatt gtctgatctc
    61921 ttttggtggt cgatcaaata gtacatgacg tatatctctt gctactaaat ctgttgctgt
    61981 cgccgttagt gccagtgtga ccgggtttcc taaaagcttt cggatttttc ctaactgttg
    62041 atattctgga cggaaatcaa ctccccattg agacacgcaa tgagcctcat ctactacata
    62101 taacgcaata ttttgttttt tcagatgctc taaaagagaa ggattagtca acatttcggg
    62161 actcaaaaac aaaaatttat attgcgatag atgacggagg acgtatcttc tttcagattg
    62221 cgaaagtaaa ctattgaaag cagctactcg tttctcccct cgtttttgta attgcgtcac
    62281 ttgatcttcc attagagaaa gcagcggtga cacgatgatg accagacctt ccattaaata
    62341 ccccgtcaat tgatagcaaa ggctttttcc cgttcctgta ggcaaaatag cgagagtatc
    62401 ttctccatca agcaacgcct gaataatttc ttcttggccg ggacgaaatg aatcaaatcc
    62461 aaaatatttt tttaattcat ttattaacat tctgtatcgc ttcctttctc aatagataga
    62521 tttgatacaa acgaaattct ccataatcta attgtccatc tacattccat tcgctaaagc
    62581 gccactctcg cacggaatcc ttatttggtt ccaaacgttc gattgtttct gcagagagaa
    62641 tccactcgta tggaaaatca tcaaaaaaca aggcccactc aataagatga tctgttactg
    62701 ttcctctttt taaatgacgg attgccatga tctggtcaat tgtctgacca ttcataaaca
    62761 tctgcttggt gatcatcatg ctttgattga aattttgctg taataatgaa tcgatcaaag
    62821 cgtgccaaag cgaatctggc cgtttttcga tttgtgctaa caaaaggtgg atacatctgc
    62881 tttgttgcag ataaaccgct gactcatcat tgttttttgc cagctgataa ggaagcagcc
    62941 ctgttcgctc atttccagat aattggttag ctaaaaaatc tgctgcttct tctggtatct
    63001 ccgaaaacag ctgcaccaaa ctctcatacg cagtatcaat caaaatttca cgtggcaatt
    63061 ggctttcact aagccatttt tttagttgga aagtataaaa aggacttgtc tctgctggaa
    63121 gatagtcttg aatacccgtt gccagattag aaattacttg aacagcaaat ttaatgagcc
    63181 gccaactagt ttcccctgtt ctgccatagc ggtcaaaacg caaaccagta tgttctattt
    63241 gatgttcgga caacatatca gcccctgcag gcgttagctt tgcttcattt tcatttattt
    63301 cgatccaacc ttgctgcgca attttttgca aaatttgata aaacttatcc tgcttcaatt
    63361 ctggaaaact gttatggata aacaaaagtt cgtgggtaaa tccataaata agtactgaag
    63421 tcgttcgttt tcccacaagg agttggtaaa gagaactaat ccgcagcttg tgtcctgatg
    63481 aaaatagcga taaaataaaa acaatcatat tattctcctt aagttaggat ggttagaaat
    63541 gtcactacta tgtaaacttg tacctgaacg ctgtattgca tgcggtcttt gtcaaatcga
    63601 agcgccagat attttcgatt atgatgaaga aggaatcgtt ttatttgccc aagaaccaga
    63661 ggctcatcaa cagttcgtga tgccggaaga agaaacatat gtaaaaaaag cgtatcaaaa
    63721 atgtccagtt cgagcgattt tattagaaaa taaaatataa atataaacga aactttaaaa
    63781 acgtacacta agttctctaa acttgaaaat aagccgggat ctgtttctct tgttccgtga
    63841 aatcccgact tattttgaaa gattcatttg ctatctatag ttaattgact agttttttca
    63901 cggaccctat aggacccatt tatattattt caactttttc agccagtttt ccaatgtatt
    63961 gacgatcaca gtttgctggt ctgcgttaga aatttctgcc tctccatctc ctttttgctc
    64021 accatagctg ccaaaacctc catgatttcc gccattgatt gatacatact ctgttctttc
    64081 cggtaaatac tgcttagctt cttgataagc ttcctgattc aatactttat cttttgtcgc
    64141 cgtgatcgat aaaacaggaa cagctgattc tgtcaatgtt ccttttttgt cgggataact
    64201 tgccatgaag aatacccctt ttaaatgttt gtctgaatga ttcttattga cataacgact
    64261 tgccattacg ccacctaatg aatggccacc gatcacataa tcggaatttg gaaaatcttc
    64321 tttgacctta tccgctttgt ttccgcccat cactgctaga tcaagtggca ttttgacaat
    64381 agcgactgaa aagccgtttt ttgctaaatc ttgcgcccaa atactataac tttctggctc
    64441 tacgagagca ccaggataaa aaataagtaa tggagacttt ccatgattct ttttctctcc
    64501 ttcaaaaaat aagtaatctt ctttgttttt tccttctttt accaacactt gcgcttctga
    64561 cgatggttcg tacgtatttt gctgtatata gtaataaccg gctccaccga gtaatcctaa
    64621 aatgatgact aagccaatta gtattcgtct aaatattttc actttctccc accttttcta
    64681 tctgagtgac ccctttttct taatcaagta tgattatacc ttaaaaaaat aagagagaaa
    64741 tgcaaacttt ccagcatcaa ataaaaaaag acggaatatc catttgaata tccgcccttt
    64801 ttgtatgttt atttttattt agcacataaa cgatacacag cttctgcata aatttccatt
    64861 gctttataca tactacttaa ttcccatcgt tcattcgcct ggtgcataaa gtcaggagta
    64921 tcagggaaca tcgcaccgaa tgccacacac tggttcatcg tacgagcaaa agtagcgccg
    64981 cctgatacaa acggttcagt catgtctcca gtaatatcgc gatatgtccc caatagattt
    65041 ttcactaact cactgtctag tggcacgtat aatgaatcaa ggaagtcaaa ttcttcataa
    65101 gtcaacccat atttttctgc tgttttagtt aatttagcta ctaaatcatc tttcttgaat
    65161 gtaacaggga ttctcatatc tacaccgatt tttgtttgtt ctggtgtgat ttccaaactt
    65221 gcaaagttca tcgttagctc gccggattgt tcatcttctg ttttgcccac gacagcttca
    65281 ccagttgcat tttcttgaac taattttcct aagaaattga ttgggccaaa atcaaatact
    65341 tctgaaagag caattgccag acgagaaata gcattcactc cttgtgctgc atctttcgcg
    65401 tggatacttt taccaagaac gacgatgcct tcaccttgtt cttcgaaatc gaattcgtgt
    65461 tttttcaatg cttcttttac ttcagaaagc ttttctccag agtaaggagc tgcatcagga
    65521 acaacattca acgctcctcc agcttttaca gaaaactcgt tcgttcctgg tcctgtcaag
    65581 taagcttgca ataagccttt ttctgcataa attaaaggaa attcagcatc tggcgcaaat
    65641 ccttgcgtaa ttccttcttc tttttcgtta tatttttcta aacaacgcca taaattttct
    65701 tcatctgtac caaagataaa acgaatacgt gtcttgaatt ccacgccagc atcaatcaaa
    65761 gctttgacgg catacattgc cgcaatagaa ggacctttgt catcttggga tccgcgacca
    65821 actaaccagc cgtcttttat agttggatcg aatggttttg tttcccaatt attttcgtcc
    65881 cctgctggaa caacgtccat atgacacaag ataccgaata attcatctcc agagccaatc
    65941 tctgaataac cgtaatagcc ttctggatct ttaaatgttc taaatcctag cttttcactg
    66001 atttccaata ctttatccaa agcttcaatc acttttttac cgaagggatg tccttgtcct
    66061 gaatctgctt catctagtac ggaaggaatt cggatcaatt cgctcaatga ttctaatgct
    66121 tgttcatgat gttgttctgt cacaaatttt ttcatgatat tatcctccta atatgttaaa
    66181 aagggtcatg tcaaattcct ccgacacaac ctcttcttct caaataatga cgttttaagc
    66241 ctgctccatc agcaagcaga atgttcttaa caaatgatga aagaaaaaac actcactgaa
    66301 tggagctatt gacttatcca cgtgcttatc gatagccgtt tatcggtttc ttcattctta
    66361 tgatagacct tgtacctgta tttgtctagg tttagaacat tgctgctaca cctaggatca
    66421 cacagatggc tacaaataat atcgcaatta atttaccggt aaatttccac cacactgaaa
    66481 catcgaaacg agcaattgct acagcaccca ttacgatagc agatgttggt gtaattaagt
    66541 ttactaaacc agatgcagat tgataagcag tgattactaa atccttaccg acgccggcaa
    66601 attcacccat aggtcccatg attcccattg ttgcagcagc taagccggaa gttgaaggaa
    66661 tcaagaatga catagggata tagaaaataa atgtaataat gatgaataca cttgaagata
    66721 atgaacttaa tccttgttcg ccccaatgta agatggtgtc tgtgattaat ccattgttca
    66781 taaccacttg gataccacga gcaacagcta ctacgattgc tacaccgatc aaatcagaag
    66841 caccagctaa aaattcagag acaaattcag actctttcat gccataagtg attgctacta
    66901 cgatggacat tactaagaat aacatagtaa tttctgggaa gtaccagtca cctaatggaa
    66961 gcatgctttt acctagaacc gtacctagta ccggaaggtt tgttaaccag tttgtcaatg
    67021 attcaaaaat cgtgaaattc gcattcaagg ttgtccaagg aatcaaactg atgatcatga
    67081 taccaaaagt aataaagaac aatacattaa catgtttttg ttttttcgtg attacttctt
    67141 gacgaccgat tgcttcaata tcgaaatgct tcatatcttc tttacgttgg ttgtatacta
    67201 atgattttgt tggatctttt tcgatctttg atgcataacg atacacatat acaatagaaa
    67261 tagctaacaa aataacgaac atcaagaatc gaagaccaat accttcaccc atcgaaatcc
    67321 caacagcttg tgaagctacg cctgttgcaa aaggatttac cgttgaagct agacagccta
    67381 cttgagaccc tactaatacg attgctacag ctactactgt atcaaagcct acagccatca
    67441 tgactgggat cagtaaagca tagaatggaa tcgtttcttc tgccattccg taagtagagc
    67501 cgcctaatgc aaataacagc atcaatactg gaataagcat tttttctttt cctttgaatt
    67561 tcttcacaac tgaagcaatc ccggcatcta gtgcccctgt tttgttgata atacctaaaa
    67621 atccaccaac aaccaaaatg aagaaagaga ttgggattgc agcaggtgtt tctttagtta
    67681 gctcccccgc tggtacacct agcatcccat tgattggcgc catgaaaata tcccacagcc
    67741 cttgaggatt ttgttgtact cgttcatatg atccagcgac gatgttgcct gcatcgtcta
    67801 cactatagtg cccagctggt ataaaccaag taagtacagc gattactgca ataataataa
    67861 ataagaccgt atacgctgaa ggcatttgaa attttttctt ttttgtttgt tccatcttct
    67921 tcttcctttc ttttccgtta gagacaacaa ttttggctct aatgttattt tatcaaaaag
    67981 agtatcagaa caaaaataat aacacgcatt ttgtaaaact tttgttcaaa aagttcgttt
    68041 ttccctattc cctcctcttt tttcaggttt ttaaagcttt cttctttttc attgtagcgt
    68101 ttcctttttt ttataaacaa acattttcgt cttttaaaag gagaagaatt tcacatttgc
    68161 aaagaattga ttgattttta tgcataaatc aaaaaaatac tcaaaacaaa caaataaaac
    68221 gtttacatta ttgcatgtct tgtatttttt tccaaaaaaa aagaccaaga aatttcttga
    68281 tctttttgat atttatttga ctgctgtatg acgttgttcg aactgatgtt gttttcgact
    68341 taaccatgga agaagttgcg catgcagcac taaaaagaca gcactcacga tgaatccttt
    68401 gatcaagttg aaaggaacga tcccaatcag tacatagttt gctaaagaca tgcccagcat
    68461 ctgtgttgcg ttcagcccta aaaacattaa gtaaagaggc gtaataacga aataatttgc
    68521 gatactcata aaaatagtta gagcgatggt tcctgtaatg acacctaaaa atttgtttct
    68581 tcctttatgc tgtttcttta cactaaagaa ataataaatc gggaatgtaa agatcgttgt
    68641 tgataagaaa ctagcagtgt ctcctaccaa attatccgga gaaaaacctg tagtcagcag
    68701 atgtaatact gaacgcaaga acgctgttgc aactccggcc gctggaccga ataagaacat
    68761 gctgatcaat accgagatat cactaaaatc gatcttcaaa aaactaaaag ccggcatgat
    68821 cggaaaagca aaatactgta aaactaatgc gatcgcagca aacatcgcta ctgcaaccat
    68881 tttctgtaca cgggtgttct tcatttgaaa tcctcctgtt aggactcctc ttcagcgaac
    68941 tttttgacgt ttctctccaa atgaaaaaac tctattttcc agggaaaata gagttagagt
    69001 tatttgatca agcgtcgtca aataagctct atcttcttta tccagactat actgtcggta
    69061 tcggaatttc accgattcag ctattttttt gcaaaatagg tcgtggacta taaccaccgg
    69121 tcgggaattt caccctgccc ctgaagacga accttatata atttttgttt acatattcat
    69181 tatacatatc tattcaaaaa acgcaacatc taacacttat aaaaagtaag tgttcatttt
    69241 gagtggttca gcaaactgct gacttctttt ttgttcagtt cacggtaatc cccaggacgt
    69301 aggccttgta aagtcagatc tccgtatttt tcacgtttta gtttttgcac aggaagacca
    69361 actgcttgaa gcatattttt cacttgatga ttacgtcctt cgtggatcgt caattccacg
    69421 atactgtggt ttgtcttagg atccactgat aaaatttgga atttagctgg agatgttttt
    69481 cgtccttcga tctttattcc ttttgtcaaa ggtgcaagca tcgattttgt agcgatccct
    69541 tttacctttg caacgtaaac tttatccact tcatgttttg gatgtgtcag gcgctgagca
    69601 aaatctccat catttgtcag tagcaagatc cccgaagtat cgtaatccag tcgacctaca
    69661 ggatagatac gttcttttac catcggcaaa agatccgtaa caacttttct tcctttgtca
    69721 tctgcaacag cagaaataac tcctttaggt ttgtacaata aatagtaacc gtattcttct
    69781 tgataaattg ggacttcatc cacttccaca gtgtcatgtt tcccaacttt tatccctaat
    69841 tcccttacaa cttgtccgtt tacttttaca cgtcctgcaa ggatgaattc ttctgctttt
    69901 cgacgagaag tgatgccagc gtgggcaatt actttctgca atctctccat tacaattatt
    69961 ctcctttatt cattccatca aagaatagat ctggtgacac gtcttcttca agtgaaattt
    70021 ccatttcttg tgtatcaggc agttcttcta atgatttcaa accaaaataa tctaagaagt
    70081 agccagttgt tccatataat atcggccgac ctggtccttc tactctacct ttttcttcaa
    70141 tcagtttatg ggcagctaga cgctgtaccg ctcctgaaac ctgaactcct cgaatctgct
    70201 cgatttcagc tcttgtgatt ggctgtttat aagcgatgat tgctaaggtt tcgacggcgg
    70261 cttgagataa tgtgtttgat gtcccttgcg catatcgctt aagtaaggga gccagctctt
    70321 ttttagtcgt taatacataa tggctttccg tttcaaaaat atgcaaagct gagtgcgagt
    70381 tttgagtgta agattctttt aattctttca ctaaacttgt gacttctgct tcgctgagat
    70441 ttaacagtcc gcttaattct tcattactga tcccttcctc gccaacaata aaaagaaggg
    70501 cttcaagttc acttaatttc gtcatgttcg gtatctccta ctgctggata aagcataatt
    70561 gattcatagt tttctttttg ttgcgcaatg atctgccctt ttttcatcaa ttctagcaaa
    70621 gccataaaag tagtgacaac ttcactttta gaaggtgact caaaaaaaga atcgaaagta
    70681 cagccatgcg catttctttt tatacgctct tcgataaata ccattcgttc ttcgatcgtt
    70741 gtgtcatcgg gcgtgatcgt tttttccact ttttgagaaa atttcttacg ttctaagatt
    70801 ttctgcatgg cacggatcaa atcgattttt ttgacttgtc ctcgcttcaa cggcttttca
    70861 gcatccgcta acccatcgat gttcataggt tcttttgtgt aataaagact tcgttcctct
    70921 gctttttctg tcagtctttc agctgcatat ttatatttac ggtattctaa aagctgagtg
    70981 accaattctt ctcgaggatc ttcttcccat aattcttcat cgtctgtgac gaattcttgg
    71041 acaggaagca atgtcttgct tttgattgcc atcaatgtag cagccatcac taagtattct
    71101 cctgcaacag ctagctcgag tgttttcatt gtatggatat aattcatata ttgctccgtg
    71161 atcgtagcga tcggtatatc atatatatcg atttccattt gctgaatcag atgaagcagc
    71221 agatcgagag gaccctcgaa tacatctaat ttaatattga tctccgtcaa ctcattcact
    71281 ctttcttctt tttctttgct agccattttc caatgtaagg ctctgtgatt tcagttgcac
    71341 ggatggagac atccgggtac ttttcctgta attcatctaa catttgaaag cccatattct
    71401 caccgcgata agacggattc aaagaaatgt catggatgat cagctgatcg ttcgttgccc
    71461 agtaaatacc aataatccct tgaatgttgg ttgaaccttc tggcgtaaag aaaaaaagtt
    71521 cataatcgtc ttcagattcg tacgtatcaa tctccttcaa caaagaagtt ttacctgtca
    71581 gtttcttatg gaaactcaat agtcccatag caattttacg gttttctttc cggtaatgtg
    71641 ttaacatctt ctcaccttcc tatgactaag cccttggaaa atgctgctta taaacatccg
    71701 tcattctttt cttcgtgata tgtgtataaa tctgagtcgt agagatatcc gcgtgaccta
    71761 acaattcctg tactgtccgc aaatcagctc cattttctag cagatgtgtg gcaaagctat
    71821 gccgcagcgt atgtggtgtt acttctttgt aaatcccagc ctctcgtact aactgcttca
    71881 gatttttcca gattccttgc cgagaaagac ctttcccatg attattgaca aatacatgca
    71941 tttcagatgc gtcctttaca agtaatggac gtgcttcgtc taagtagcgt tccagccatt
    72001 ggatcgcata atcacctaaa ggaatgatcc gctctttatc tccttttcca actgtctgta
    72061 ccagtccaag agaaagatga agatctccca atttcaatcc aatcaattca ctgacgcgca
    72121 tcccagtagc atacatgact tccaagattg cacgatctct tatccctaac gtttttgtcg
    72181 tatcaggtgc ttcaatcagc ttttctactt ctgtcagagt caatgtactt ggtaatttct
    72241 gtgctttctt cggcgtatcg atatgttgca tcggatcatg atcagtgata cgctcttgcc
    72301 gtaaaaactg atggaatctt ctgagacttg agatcatccg agtaacagtt gctgtcgctt
    72361 ttttttcttc atgcaacttc tccagaaaac ggatgactaa aaagcgatcg acatcctgcc
    72421 atgaagcaat tttctgctcc tgtagaaaat gcaagtactg ttccaaatca cgttcatagc
    72481 tttgtcgtgt attggcggac aatccccgtt caatattcaa gtagtggaga taatcgttga
    72541 tttgctcttt cataataatc cccctcatgc ctctctatca tatcagatac cgcttcattc
    72601 tgatagtctt tttccataat ctgagcaaag aaaaagccgt ctagacactc agacggactg
    72661 gtttcttttt tctttttcat agcattcctg acagattcca tggaacgtta gccgatgatc
    72721 ttttactaaa aaatgataac gttcttctac tacttgctcc acttcaagta aaagatcctc
    72781 ttctacttcc tcgatcgtgc cgcagttcaa gcatagtaaa tgatgatgga aatgtttggc
    72841 gccttctttt ctcaaatcat aacgggctaa tccatcatta aaacttactt tatcgacaat
    72901 tttcaattct gtcaagattt caagggttcg ataaacagta gcaagtccaa tttccggact
    72961 cttttttttc actaaaaaga agatttcttc tgcagataaa tgctctttct cattttccaa
    73021 aagcacaagc agagtagctt ctctttgcgg tgtcagctta aaccccgctt catgtagctg
    73081 ctgttttgtt tttttcatcg ccgaaatagc attcatgacc atttactcaa ccccaacaat
    73141 ttataataat tacaaataaa aaaagatata acactatgtt aattaaaatt attataagct
    73201 agttaagaaa aacatgcaag ggattctcaa ttagattttt tgactagttt tgtcacaccg
    73261 ttttcttgtg taatcaatcc ttgtttcaaa agattaccta atgcgcgctt aaatgctcct
    73321 ttgctgatac caaaagcttt catgatttct tctggatccg atttatctgt aaagttgatt
    73381 tgctgatctg cagaacgttc taaaatggtt aaaatcattt gtgcatcatc tgatatagct
    73441 tcatatgctc ttggcttcaa tgaaagattc agtacaccgt ctggtcggac accaatcact
    73501 cgacctttta tctgctcgcc tagacgaggt tcattatatc gttcagaagg atggataaac
    73561 ccaagataga aatcttccgt taaaacgtac gtacctgtta atttcaagcg gtatgctgtt
    73621 cctatcacat ctttgttttt catttcttct gttccatatt tgcttaaaga tttgaatatt
    73681 ttttcatctg ctaaactgcc ccaaatacgg tcttttgcat caactctaag ggaaatcatc
    73741 aagcgatccc cttttttagg ccatagctct gtcattgtcg gcaattcatc aagagatacg
    73801 acaaagtctt tatctggaag accgatatcg acaaacgcac ctaaatcttt tcgtgtagca
    73861 gtaacgattc caaaagcgta atgaccgata cggcttttag gaatttgtgt agtgatccga
    73921 ttttcatgtt tttgattttg gtaaccgaat ccttcaactg cttcaccgat tttgtgttca
    73981 ccttctgatt tgcttaagcc aaaggtttgt ccgttttttt ggacaaagta gtttttttca
    74041 ttttcatcaa taataatacc tgtaaatata gttgctaata aatcgttcat ttttttctcc
    74101 ctctatagtg aaaggttttc ttaatacatt tctttgctat cataacataa tgttatctac
    74161 ttgaatagct gagccttggt ttggaaaggt gaaaaaagga gtatgacaac agttgtgtca
    74221 tactcccctt tttcgaataa acggtgttaa acggcaggct cacagcctct ttcatttgcc
    74281 taaaattaat tgctcccatt tcctccgaac catccagttt tacagttgat ggttacgatg
    74341 taaaatggca aagcactttc tccaatgcct ttcatcaatg cagattttgt tgaagtggca
    74401 tatagattat aatagccttc cccattttcg attctggctt tttctaatct gtaacctcct
    74461 tcaacgatat atccacgttg gatacatgta gcaatcactt tgctttggac acctggtgcc
    74521 cacgtccatg ctggattaga tgcatcggca atatcacttg ctctggcaga tttgattggc
    74581 tgattcgctg agtttgaaga ggaaggggtc gaggatgccg cttcttgctg tgctgcttgt
    74641 gcgaccgcag cttgttgagc ggcttgagct gcagctgctg cttgagcggc ttgttttgct
    74701 tgttcttcag ctgctttttt cgcttcttct gcttttttct tttcctcttc ttcttttttc
    74761 tctttatcag tgagcatctt atctacttgt ttcaattgtt cattcaactg agcagataga
    74821 tctgtatttt tcactttttc tactgcttct tttgccgtct tgtattggtc gcgtgttgct
    74881 gaatccacta cttttccgtc tttaaagact atagctgttt tttctttagc tgtttgcaat
    74941 tcagcatatt gatttttagc ttctgtttgt gcttgttgaa tcaattttcc aaaatctgtg
    75001 ttatccgttt cgattgcttg gatctcttta tctgctttca acttgatgtc atctgctaga
    75061 tgatcatcga cgatggcagg tgctatgaac cactcattca ctttttggat atctcggatc
    75121 ttagattgga tatcttggta agttgtttct aactctgaat atcctttttc attcttaaga
    75181 ctggataaat cattttttag ttttgataaa tcttgatgaa tcatatctgc acgaataaat
    75241 tgatggtgat catctgtata aaaggcagca agtttctcat gaagttcttc catttgttgc
    75301 tgtctttttg cttctgctgc tacttcggct tctacttttt gatgatggtc ataataagcc
    75361 cagcttcctc caccgagtag aagtactgct gctaaaacga gataaggtac tttttttgat
    75421 tttttctttt gcgctttatc tggatctgtt ttttctaagt ttgattcttg atgtgttttt
    75481 tcagtatctg gcacgattgt tattgatttc gcttctgctt caaacagctt aggctggttc
    75541 tcttcttttt ctatttttgc ttcaagatca gtaccctctt tgcttacaga ttgattttca
    75601 gcatcggatt cactatttat ttcatcgggc tctgttgact ttttgtctgt gttttcgagt
    75661 atttcttttt tctctgcttc cggcagttta ttcgtttttt ccttttctaa ttgtttttca
    75721 gtaaatactt cagttttttc tgtgatatcc gtttttacgt cttttatttt tactgtttct
    75781 ttacctgtgg cgttttctct atcagagtct ttttcagttt cttgttcctt ttctggctct
    75841 actttgtttt cctgtgcaat tttttcactg atcattttga gcagctcttt cgcgtgctct
    75901 tcttttgtat cttcacgatg tgctttgata taagcggata gtatcgaatt ttcttcctca
    75961 ttatcgacga tttctttcag ttcagctact cttctttttt gttttgattc aagcgtttct
    76021 tcttgattct ttcctgattc ttccgaagtc tcatgaacag ttgctttctt ctgttcattt
    76081 ttctctatat tagttacttg atcgtgagca ggtggttctt cgtcttctgg ttctgctttt
    76141 tcgaataatt ccattacaga ttctagaggt aaatctttca attccgacca ttcgattgga
    76201 tcgtttaatt ctgtatcagg aaagactgca tcttcagttg atttatcctg catagctgca
    76261 tctgtatttg tttcttcaat tgattctttt atttgctgat cttctgcagt tatttgatga
    76321 ccgcagtctg gacagatagt attctgctcc atttttttcg atccgcaatt tggacatttt
    76381 ttcatcactt agcgatcggc tcctttacta ggtttacgct accatttaat ctatcatatt
    76441 ctatacgtca cgccaatagc ttatttttag gatttactta aaaataatga atgaatttcc
    76501 ttcctataga aaaaaaagct tgagactgcg tagtctcaag ctttttttct tttaaaataa
    76561 accaaacagt tttactgatt tctcttctgg tcaatagtcc acgcttattc taagaaatct
    76621 tttaattgtt ttgaacgaga aggatggcgc aattttctta atgctttagc ctcgatctga
    76681 cgaatacgtt cccgagtaac accaaatact ttccctactt cttccaatgt tctcgtccgg
    76741 ccatcatcta aaccaaaacg taagcgaagg acattctctt cccgatcagt caatgtatct
    76801 agtacatctt ccaattgttc ttttagtaac tcataagctg cgtgttctgc tggacttgtt
    76861 gcttcctggt cttctatgaa atcacctaag tgagagtcat cttcttcacc gattggcgtt
    76921 tcaagggaaa caggttcttg cgcgattttc aagatttctc ggactttttc tgttggaaga
    76981 tccatttccg caccgatttc ttctggcgta ggttctcttc caaggtcttg cagcaattga
    77041 cgttggatac gtatcaattt attaattgtt tcaaccatat gaactggaat acggatcgta
    77101 cgtgcttggt cagcgattgc tcgagtgatg gcttgacgaa tccaccatgt agcataggtt
    77161 gaaaacttaa atcctttgcg gtagtcaaat ttttcaactg ccttcatcag tcccatgttt
    77221 ccttcttgga tcaagtcaag gaactgcatg cctcgtccta cataacgttt agcgatactt
    77281 actactaaac gaaggtttgc ttctgccaga cgctgtttcg cttcttggtc gccttcttcg
    77341 attttcaatg ctaacgctac ttcttcttcc gcagttaaaa gggagacacg gccaatttct
    77401 ttcagataca tacgaaccgg gtcattgatt tttacaccgg ttggcgcaga aagatcttct
    77461 gtttttgctt gttcagcttt tttctcagca ctcttcaagc tgtgaattga tggatcacca
    77521 ttttcatcca ccacactgat acctgcgtct tcaatctttt ggatcaattt gtccatttca
    77581 tctgcattca aagtgaatgg tgtagcaagc tgattggata aatcatcata tacaacttgt
    77641 cgtttgggtt tgttttcttt gatgaaagct gctactgctg cttcatacgt ttttttatct
    77701 gttccatttg ccataaaaga aggcccccct tgagtagcta tttggcttgt tttaactgtt
    77761 tcgttaaatt gatgatttca atcgtcaatt caagctcaag ctgtttgttt ccagttcgtc
    77821 tggcttcttg ctgcatttgt ttttttaatg ttatttcatc agctaagcca gatttgctga
    77881 tcacatctag caagtcttgg atttctcgtg gcgaactttc ttctgccacc ggtaaatatt
    77941 cgatttccac caccaaccgc cttaaatttt catcttgcaa aaagtctaaa aactctgcaa
    78001 ggttgaattc ataatgttgt gagacatatg catcgaataa aaaataaact tctgcatatg
    78061 catcatgaat aaactgaaat tctcgttctt ttaaaatatt acggacagaa acttctttaa
    78121 atgcacgata taatagaatc tgctctgcct tttctacttg cgtcaaaggt ctctttttcc
    78181 gtgcagtttc ttgtctcgta acctgagtgt gttgactttc ctgaggttct tgacgatttt
    78241 gctgatttgt ccttcgaaga tcgcgcagtt gtttttgtat cgtctctctg ccgatctgaa
    78301 actctgttga cagttgagat agatacatat cttgttcgat tggtgaaaca actcggctaa
    78361 gttcaactag taattcttcg aggtattcga tcttgtcttt ttcattatct aaatttttcc
    78421 cttgttttaa ataacgtgat ttaaaagtaa agactgtttc gcgcccatga gccactaatt
    78481 cttggaaaga tgcttctcca tacttacgga gatattcatc aggatcaagc ttttctggaa
    78541 cgactacgac agaaagtgct aatcgactgt tttgccgaag caattcaatc cctcgattgg
    78601 ttgcttcaaa acctgcgttg tctccgtcat agcaaaagac caattctttc gtcagctttt
    78661 ccatttgttg gatctgttga gctgttagac ttgtccccat agaagctaat ccagttttga
    78721 taccagcctg ccatgcagca atcacatcca taaacccttc gaacaacaaa atttctccgt
    78781 ttttccggat atcgcttctt gctttatcaa gattaaataa aacttgtctt ttattgaaca
    78841 gttctgtttc tggactattc aagtatttgg gctgatcttt ttcctgccct ttttcaggtg
    78901 caagccagcg tcctgaaaaa ccgatcgtct ggccttgaga attacgaagt ggaaacatca
    78961 ctctttgata aaaacgatca gacatacggc catcatcatg cgtgacaaac aatccagagc
    79021 gttctaacaa ttcatttgaa attgattcat tcttgaatac ttgctgaaga aaagaacgct
    79081 cattaggtgc aaaacctatg ttgaattctt caattaattc taaagacaac ccacgacttt
    79141 gcaaatattc taaagctggc tgtccagctt ttgtatgcaa tagcatgtga tgatagattt
    79201 ccgctgcttt ttcatgagca gcaatcagtt tccccgtagc agattcagga tttcggacag
    79261 ctatagtacg ctgacgccat tgatcttcta acggtatctg ttcgaagtct gccactttta
    79321 ccactgcttc tggaaaactt agaccttcta gttcttgaag aaaagtaaac acgtttccgc
    79381 ctttaccaca accaaagcaa tgaaaaatct gcttgtcttc tgcaacagaa aaagacggtg
    79441 tacgttcttc atgaaaagga cacagcccca agtaattttt accagacttt ttcaactgga
    79501 catattgtcc aatcacctca acgatattcg tttgactgcg tactgtttcg ataacctctt
    79561 gaggaatccg ttgagccatt ttttcacccc ctgctctatt gatatctttt gggtttaaca
    79621 gaagatattt caggagaata accggtaaaa aatcgcacta actgaaatca gtgcgatttt
    79681 aaaggacaca attatacatg tttgattgta tcatatttca ttgctttttc aagtataaca
    79741 catttctatc tcattaagca tcaatttgct actcttttca gtaactgatt ttgaatatca
    79801 gccttccagt acaatactcg aagaacaata tttacgagca tgaatacaaa gaagtatcct
    79861 acgctctctc ccaaccctaa gtttacttcc aaaattccat ggaacagcat tgcagctaca
    79921 tatatcccta cgatcgtcgt tactgctagg aagatgcgca atgggttgaa gggataacaa
    79981 gcttttacaa ctgccatgca actgatccca atcagcaagt aatacatcaa agtagtagtt
    80041 tcagcatttc cccatgcgaa tgtttttcca agtagataca ccgcaatgat attcactact
    80101 acaagcaagg catttggtaa agcattaata agtgctgtag gcaaataacg gtaagtgatt
    80161 tttcttttat cttgttcaaa agataaaaag aaggaaggat acccttcaat cgctaaatca
    80221 atcagcgtga tttggatagg gataaacgga aatccaattg ctgtaacggc acatataatc
    80281 gataaaataa atgaataaat cgttttgata aaaaagatgc ctgagacttt cgtcacattg
    80341 ttgaccactc ggcgtccttc aaacaggact tctggcaatg tagtaaaatc agagtccaac
    80401 aataccagat tcgcaatctg acgtgttgct ccatcacctt ctgccatagc aatgctgcaa
    80461 tctgcttctc ttaacgccaa aacatcattc acaccgtcgc ctgtcattgc aaccgttctg
    80521 ccgctttctt tcaattcatt taccagcaat ttcttttgct gcggactcac tcgtccaaaa
    80581 accgtatagc gatgagcagc ttctcgcact tccatttctt cagtaatttg agaaagatcg
    80641 acatacgcat cataattcgg taatcctgca cgtctagcga tattggagac agtaaaagga
    80701 ttgtctccag aaatcacttt cagatccaca ccttcctcat gaagataagc tagtgtttct
    80761 tgagcgtttt ttcggattgg atcatcgatt tcaaacagag caaccggctc aacaaatgct
    80821 ggtttttctt cgttcaattg ctgatttgga gcgatcccca acataagtac gcgatagcca
    80881 ttcgcttggg ctgtttttag actgtccgga agccgatctt cttccaccaa acgttcaggc
    80941 gctcctacgt acacgactcc cagttctgga aattccatcg cgccccattt gcgatcagaa
    81001 gaaaatgcga ctttgtttgt cacatcatac cgattagaaa gctcaaaata atctctcata
    81061 gcccgcatag tgatgttgtt atcactgctt tctgccagat acgtccccat aatttctggg
    81121 atggctttct cgtacagcgg atgtagtgct tctacccgct ggatactcat tttcccttca
    81181 gtaatggtcc ctgttttatc caagcataat gtatcgacat gggccagcgt ttcaattgaa
    81241 tacatgtctt gaacaaggat tcgttttttt gccagtttgg ttacccctgt cgttaatgca
    81301 atacttatta gcaataccaa accctttgga agcatgccta acaaagctgc tgcagatgca
    81361 ataacagaaa ttttgataga agcatcgcgg atgaacagtg cttccaaaaa tagtatgatc
    81421 cccaaaggaa taatcacgaa actagtgaat ttggatactt tgcgaatcga atctacaagt
    81481 tcagaacgaa tagctttgtg agtctttgcc tcgctagtca agcggacagc ataattatct
    81541 gcacctactc ggataacttc agccatgaca gaaccgcttg tcaaaaagct tccagaaagc
    81601 aattcagctc cagtttcttt gacaatcaaa tcagattcgc ctgtcaaaag agcttcattt
    81661 gcttctgccc gtccagatat aacctgtaaa tcggctggta tctgttctcc tgctccgatt
    81721 tttaccaaat ctcctaatac cagctcttta gtagaaatcg attgttcttt accgtcccgg
    81781 atcactagta cttctgattg ggaaacaatc gataatttcc gtacaagatt tcttgcatgt
    81841 atttcttgat aaattccaat aacgatattg acgacaataa ttgctaaata cgccatgttg
    81901 gaatacgcac ctacaaataa cagacaaatc ccaataccaa agttcagaaa attgaataac
    81961 gtcaatatat tatcgctgaa tatttgtttt gtactttttg atgcatcttc ctggtagtca
    82021 ttttgctggc cagaagccat tttctgaact acctcttgct gtgtaagtcc attcattttc
    82081 tttttctcca ttttccgcca actttcaaaa ataatcacgt gtttaagtct agcgtgttcc
    82141 aagattaaaa acaacttgtt tttataaaat ttatagaaaa acacacgaat gattttacaa
    82201 aaaagatgcc aacctttttg tttgaatcac ccgtgtgctg tttttattat caatctatct
    82261 ttacttatat tgttcctcca aacggctcat ccaccaacct agtgctgtac cggctataaa
    82321 aagaacagga caagctagta ccccagcaac tcccaatccc gcgtaatcgg taggaatgat
    82381 cataatcgtt gaagcaaaaa ctacacctaa aatgaaatgg aatagcttgc tatatgcctt
    82441 tgagaaaatg taatccatga tttttgacaa tccaagaaca caaagaatcc caccaattcc
    82501 gatcggcaca atcacttcaa gtcgtacttc tttaaatccg tcagccatcg ctttatacaa
    82561 tcccatatac accaaaaaat tagaagggct caatcctggg acgatcatac ccaaaccaat
    82621 caatccgccg gcaatcagcc atgttccaaa attctgatcg acttgcccgt caaataattg
    82681 agcgccgtat aataaaaaca aataagccag aacaaaagta acaaccatga tgatcacatc
    82741 agaagtatct cttccttttt tccctgcttc tttccataac gcaggaacag ttccagcgat
    82801 acatccgaca aaaaaccata atatcgttgt ttcatacgca cctaataaaa aactgactaa
    82861 aaaagaaagt agaaagatgc ctgtcactcc gcctagacca acaggcagaa aaaacaaaac
    82921 attttctttg aaattttttg taaggtgggc taaaaaagaa atcagccgct catacatgcc
    82981 aaagacagct gctaatgcac caccgctgac tcctggcaag ataaatcctg aaccgataaa
    83041 cattcccttc acaaaccgta acatccagtc tttggttgtg taccccactt cttgattttc
    83101 cattttttgc cttttttcca ctatttgcgt actccttctt tcatttaatt ttattcatga
    83161 ttgaacattt cctgctattt tacttaaaga ctttttcata ggtctctatt attttttcat
    83221 tcattttgac aatatcagga taaaacatgt tacctccatt atgatagaga tagccgccat
    83281 tgtaaagcaa ggactggatc cgccaatatc ggtattgttt cccttcctca tttcctaaaa
    83341 gtgggctcag cacatcttta gaatacgctt ctgctaattc tactgtgttt tctccgccat
    83401 gattttttac ataggcaata taatctttcc caaaattata tgcttggaca gctgtccata
    83461 aatcgcatcc ttgtttcttt gcttctacta tcatttccgc caaatatttg acaccttgat
    83521 caatactgtc agctggatta tcaatgatct cagcttctcc atgcagactt tccgagctct
    83581 gcatgagatc tgtccctttt ccttttgatt cggtcaatat tatcgccttg atcaaattct
    83641 cgtagttttc catatgataa ttttttgcag ctgttgttat ttctatttga tagctttcca
    83701 ctcgttttac cgaacgataa gttcttcctc ataaaaacaa caccgcacca ataaccgtca
    83761 gaatgatcat gtaaaaaaga atacggataa tagataatcg ttttcgcttc atttcatcac
    83821 ctagttattt tgacatcttt ttttatctta ctatttcttt atcttcttgg ctatttcttc
    83881 cttgatttct gtgtacgcct taagaaaaaa tgaattttcc tgtatttttt gacatcttac
    83941 gaaaatgatc gttgtttttc agtaaaaaca attgcccaat gactgatata atcgttatac
    84001 tttacacagt aggtgattat gtgaaaaaag aaaagaaata tgcaaaaatg ccagatggga
    84061 gcgaaattta ctatgaaaag agcggtcaag gcttccctct ctttttactg catggcaatg
    84121 acggcagcgg ccgctttttt tcagaacagg tccctgtact cgaacgctac tataccgtgt
    84181 atctagtaga tagccgagga catggtcgtt cgacaaacga agcatctatg ttgaattttc
    84241 agttaatggc cgaggatctg aatacgatca tgttgctgga aaaaatcgat caggcagatt
    84301 ttcttggatt tagtgatgga gcaaatctag cactggtatt tgccagcagc ttccctaaaa
    84361 aagttcatcg attgatttta aattctggaa acacgcttgt taaaggcgtt cgtttttctg
    84421 ccagagtcat cagtaatatt cattatgcat gggtatggtt actctcttta tttcgcccaa
    84481 gtcttcgcaa aaatctttta gttatcaaac ttttgctgca tgacatcggg cttacagaaa
    84541 atgatttaaa aaaaatcaac tcccccactt tgatcatcgt gggaaaaaaa gatgtaatca
    84601 agctaaaaca ttctttgtat atagctaaaa ctataccaaa agcttccttt gtgcttgtga
    84661 aagaacaagg acatgaatta gctagaaaag atcctgaacg cttcaatcga gaagttcttc
    84721 aatttctttc tgaaacataa ctattaggaa tagaaggtga tttatttgtt aaaaaatacc
    84781 atacaatggc tgaaaaacca cctaggactg ttcaaaacaa tctttttgat ttctgtcatt
    84841 gtcattattg tcggtgaatt gatgtccatc ggtaaaactc tatctatcca gcaattagga
    84901 gaaacgtttg ccactatacc tgtatggaaa agcggactaa tgctattgat tggtcttatc
    84961 tctgtgttac ctatggttgg ttatgatatt attttgaatc ggatgcttga acaaaagcag
    85021 aatcctcgtt acctgtttga gacaagctgg ctgatcaata cgatcaacaa tattgccggt
    85081 ttcggcgggt ttgttagtgt ggggcttcgc tctgaactat atggacaaaa gaaagagagc
    85141 agaaacgtga tccaagcatt atcgaaagtt ttcttgtttt tgatggctgg actttctatt
    85201 tacagtctgc tttctttttt acttgtagtc ttcacgccag ttgctccttt tttgaagcaa
    85261 tattggatat ggttgatcgg cggcggtcta tattttcctg tcgtcttttt atttactact
    85321 ttcaaaaaga aaggattcct tggtggtacc tcacttaaaa ctcaaggaca gctattgcta
    85381 gtttcattgt tggaatggac cggtgtcctg tttagtttcc tttctgttgg ttatttaatg
    85441 gaaatccgga ttgatctgtg gcaaacaatt ccattattca ttgctgcttc agtgatagga
    85501 atcgtgtcta tgattccagg agaaattggg agtttcgatg taatgatgat tatcgggttg
    85561 tccgctattg gtgtgccaag agaaatagtc gttgtgtgga tcttgctcta tcgcctgttc
    85621 tactacatca ttccgttttt gattggaatc gtctttttct tcaaaaatat cggttccact
    85681 tttgatcagc gttattctgg tatccctaaa caattagcaa cagaaattgc gcataaaatc
    85741 gtggttgtct tattatactt ttctggaatc atgctagtac tttctgccac tatcccgcaa
    85801 gcttttacag agtttcgctg gcttcatagt ttgaacccat tgaaatttca ttttattatc
    85861 caatttccta gtatactatt gggatttctt ttaatcatta tgggcagagg gatcgcagca
    85921 cgtgtgaaac gagcatattt gcctacaatc ttcttgattg ttctagcttt actttatgtt
    85981 ttattaagtg attttagttt cacaccggtg gttttcttgt ctattctgct tctgatcatt
    86041 cttgcttcta aaaacgaatt atttcgggaa caattgatct attcatggga gtggcgtacg
    86101 atcgatggaa tcattattgg tgcactgtcc ttgctttaca tcgttattgg ggtatacaat
    86161 ctgccggatt tccctcatcg gcgtcaccat tttatttcct ttttcttatt tccttctgaa
    86221 aagatttggt tttcgggatt gttggccatc atcgctgtta gtttcatgat cgttctattt
    86281 gttcatttcc ttcaaggaga aaagaaacag attggcgaag cctttaatga agaaaaggcc
    86341 ctgaagatcc ttacaactta tggaggaaac tccgatagtc aattgatctt cttaaaagat
    86401 aagcggatgt ttgcctatga aaaagacggt gaaccaactg ttcttctcca attcgcttgc
    86461 ttcaataata aatgtattgt aatgggtgat ccttctggta agaaagaaga ttttccagaa
    86521 gcaatcgaag cttttatcga agaaacagat cgactatgct atttaccagt tttctacgaa
    86581 acaagcgaag agattgttat gattttgcat gaattcggct acgatttcat taagatgggg
    86641 gaagaagctt atgtagacct gaatagtttt acaacttcag ggaaaaaaat gaagggcact
    86701 cgtgctgtct tgaatcggat cgaacgagaa ggatttacat ttgatgtctt acaaccacca
    86761 ttttctactg agcaaatgag tactttcaaa aatatctctg ataattggtt gggttcacga
    86821 aaggaaaaag ggttttcttt aggctttttc tctgaagatt atctacaaag agcgcctatt
    86881 gcagttgtca aaaataccca agaagaaatt gtcgcttttg caacgatcat gcctacttac
    86941 acgaacaata aagtaggaac gattgatctg atgcgatatg attctaaaaa agcaccttct
    87001 ggcagtatgg atttcttgtt cttgcatttg tttgcctata tgaaagaaga acatatccaa
    87061 tggttcaatt tagggatggc ccctctttca aatgtcggaa cttccagaaa gagtttttta
    87121 caggaaagga ttgcatactt ggtttacgag tttggttctc atttttattc tttccatgga
    87181 ttaagagaat ataaaagcaa gtacgctaca aactgggtct cacgttatac tctatattct
    87241 agagatagtt ggattgcata tgttatgatt gctttgctga ttattgataa tgcgccagtt
    87301 gagcagacac aaggtagaat aagtggtttg aaaaaatgga tacgaagaaa gtattaaaaa
    87361 ctgcataccg atagatgaac actctcatga tgaataaaag agactgaagt aaaacgaaag
    87421 ttcatgcttc tacaatctct tcttatacaa ttgaagaaca aagagcggtc caaacagaaa
    87481 tcctgtttgg accgctcttt tattttaaac atttttcatt accaaaatat tcgagtcgcg
    87541 tttcttatgc ttcgatctct gtaacgatac ctgaacctac tgtccgtccg ccttcacgaa
    87601 ttgagaatgt cgttccgttt tcaacagcga ttggatggat caagtcaaca tcaatcgtca
    87661 cgttgtcacc aggcatgacc atttctgtat cttctggcaa tgtgattgtt cctgtaacat
    87721 cagttgtgcg gaagtagaac tgtggacgat agttgttgaa gaatggagta tgacgtccac
    87781 cttcttcttt tgtcaacacg tacacttctg ctttgaattt tgtatgtgga gtaatggaac
    87841 ctggtttagc aagtacttgt ccacgttcga tatcgtcacg ttggatcccc cgtaacaaca
    87901 cgcctacgtt atccccagct tccccgtaat ctaacgtttt acggaacatt tctacacctg
    87961 ttacaactgc tttttgtgtt tcaggtttga tccctacgat ctctacctca tcaccgacac
    88021 gaacagcacc acgatcaata cgacctgagg caacagttcc tcgacctgta attgagaaga
    88081 cgtcttccac tggcaatagt aatggtttat ctgtatcacg ttctggtgtt gggatatatt
    88141 catctactgt atccatcaat tccataatag cagcttcagc atctggatcg ccttgcaatg
    88201 cttttaatgc ggaacctttg atcacaggag tgtcatcgcc tgggaaacca tattcgctca
    88261 ataactcgcg aacttccatt tctaccaaat cgatcaattc ttcatcatcg actaaatcaa
    88321 ctttgttcaa gaaaacaatc aggtatttta caccaacttg gcgtgacaat aaaatgtgtt
    88381 cacgtgtttg aggcatcgga ccgtcagttg cagaaacaac taagattgca ccatccattt
    88441 gtgctgcccc tgtaatcatg tttttcacat agtccgcgtg tcctggtgca tcaatatgcg
    88501 cgtagtggcg tttttctgtt tcatattcta cgtgtgcggt attgatcgta atcccacgtt
    88561 cacgttcttc tggagctgca tcaatactag catagtcttg aggatttgct aggccttttt
    88621 tgcctaatac tgtagtgatc gcagcagtta aagtagtttt cccatggtca acgtgtccaa
    88681 tcgtaccaat gttaacgtgt ggtttacttc tgtcataatg ttcttttgcc ataatttaca
    88741 aacctcctaa ataatataga tcactgaaaa atttcagctc tcaccttttt attatagtct
    88801 ttttttgact aaatcaaaaa gaatacgtcc gacttacttt ttgtaagatt cgaactaagc
    88861 ttattatacg tcaatattgg tcaaagtcaa ctaagagcac tcgtctgtct tatacgcgaa
    88921 aaaagtacct tttataaata aaaaaatgtg ttttttgaga aaaacttcaa gatagtcctt
    88981 aactttcttt tgaagcaata agaacaaaat gaggaaaagt ttaaaattca gctgttgcca
    89041 acaactttca atagacgaaa tcttctattt cgttaataat aaggaaagag gtgaagtaca
    89101 tgaaaaaatt aattggcgta cttgtcgttt taggcatatt tctcggcgga agttttgcag
    89161 cttatcatta tttttacggc ggcgaagctt attatacaaa aatcacgacg aatggcgaaa
    89221 tgtcagctac ccaagcagat aacggagaga aattcacgac ttatacctac aagcaagcgg
    89281 cttataataa agatggtgaa aaaaaacagg tgacgcttcg cgaagaaaga gaacatccat
    89341 taaagatgaa tgcgtatctt aaactcaaag tcaatcctcg taaaggtgta ttaagctggg
    89401 aagaaataaa ggcttctgaa gtgcctaaaa atgcggcaga aaaaatcgat aattagtaaa
    89461 tggtcttaaa attttattag ataaaaagag gctgggacag aagcgtttaa ctccaagaaa
    89521 taagaaggag ttgcttctgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg
    89581 ttctttactt ttgtctcacg ctcttttttt gttatattcg tcttttccga tccttctctt
    89641 gacacaattc atttaactga tcactggtac ttgaacacct aatattctag tcatttttat
    89701 aaacgattca atacttcatc cacatttttg atcatcacgg atatcgtacc atccggatta
    89761 ttcacaaatt caatcaaatc tgggttgcga tatacatcta ctggtacgat cagttcgatc
    89821 ccgtttgata gcttgaattt ttgttttcca aattttttct cagaaatttc ttgtacttct
    89881 tttacaggag gtgcttctgg gacaaatcct tgctcttgta cttcttcttt aaaagccatt
    89941 tttgccgtga tgttttcctt gaagactttt tccgcaatca tctcatgatc caaccgacct
    90001 ttttcttcta tcgtatcata gacagcttct ttcaaatcag ccatgatatc aaattcttct
    90061 gtctcaaatt tttttccaag atgcttggct actttttgga tcacttttac attttcttct
    90121 aaagaaggtg caggaacact ttcgatcact cttccagaaa aataaagctc ttttttgcca
    90181 gaaaactcat attttttttc aattaattcg taggtccaat cagtcaaatt cacagcgaat
    90241 gcttcatctg ctttttgtgt tttcgcccct agaattgctc gattcaaaat caagcggttg
    90301 tcgatccctg cttcgtctgc ttcaacaaag tgagtgtaag cctcacgata attgactttc
    90361 aataaagata agtacatcac tgtatctaat tcatacaaga caacaaatac atctgcacta
    90421 ggtgcttctt cactttcttg gtagatatcg aaccaccgct gaacgattgc ttcacttgct
    90481 tccacaaaat tttcttgagc gattttaagt gattcaacga tcatgctttc ttctgctagt
    90541 tgtccagttt ttgtttgagc agaagagatt ttttggatct tcttttgaag aaaatcacga
    90601 atatattctt ttgtcagatc cagctctttt tgtgaaaaga ctggagaacc agtttctctg
    90661 tcaataatat gcagaattgc tttttttaaa tagatatcca ttttctcatt cccttctgtc
    90721 ttagtttacg gaaaaaaaat caaaaatacc agaatcaaaa tcattttctt ttaaacaaaa
    90781 atttcaagag tagaaacaaa atggattctt tttttcgaaa aatgcgctac acttatagtt
    90841 gaacgaataa aaaaggagct gaaagtatga attcattaac aagcacttat cgtctatcaa
    90901 atggttacga aatcccggta gtaggattcg gcacatggca aacacctgat ggggatgtcg
    90961 ctgtttcctc tgttaaagaa gcgctggctg caggttatcg ccatatcgat actgcccaag
    91021 gatacaagaa tgaagaaagc gtcggccaag cgattaagga aagtggaatt cctagagaag
    91081 agatattctt gacaacaaaa ttatggaatg caaaccacag ctatgaactt gtgatgtctt
    91141 cttttgaaga atcattgaag aagctccaaa cagattattt agatctgttt ttgatccact
    91201 ggccaaaccc tgttgctttt cgtgataatt gggaacaagc aaatgcagat acatggcgag
    91261 catttgaaga actgtatgaa gcaggtaaaa tcaaagcaat cggtgtgagc aatttcttac
    91321 cccaccatct agatacacta gcaaaaacag caaaaattat gccgatggtc aatcaagttt
    91381 tccttgcacc aggcgaatta cagacaactg tagttgagta tgcaaaaaaa catgatatga
    91441 tcctcgaagc atatagtcca ttaggcactg gtaaaatctt cgatgtgcct gaaatgaaac
    91501 aaatcgctga agcacatgac aagacgattg cacaagttgc tttgcgttgg tcgctgcaac
    91561 acgaattcct tcctcttcct aagtcagtga caccaagcag aatcaaagaa aatactgaat
    91621 tatttgattt tgaattaaca gaagaagaaa tgaaacaaat cgatcagtta gatggtgtag
    91681 tcggcaaagc aaaagaccca gatactaccc aattttaagt cgaaaggtga ttttatggat
    91741 ccaatcgaac gcttgaattc tctctcggaa gaagttaccc agacttttca ttccgatttt
    91801 gtctttttga tcgatgcaga gaaaatccaa cattttcctg caagaaactg gacacatgat
    91861 caaatcatcg aagaactgaa aaaacggttc gatcattcgc taatggtcaa accctggcac
    91921 gaacatgaag tcatttattc acctgaacta cctgtttttg ccttgatacc gaaaaaatag
    91981 aaagaatgga acggattatg tatatataca taatccgttc cattttttat ttttctttca
    92041 aaaaacggga aaatacgcaa atttttctgt tgtaatttgc gtatacgcaa gtttttttgc
    92101 attactcctt ttcatttggc tgtttttttc ttataatcga aagggtatga gcgaaggaga
    92161 gaacatatga agaaaaaaca gaaacaaaaa ttattaaatc aattccgtcc ttcattggat
    92221 ggtgtaagag gacaattatt ccgttctcta gaagaagaga gcttgcgacg ttatggtttg
    92281 ccgttacaag tcatgcttga tcccaaaaac cgtcaaatct taacgatcgg attagctggg
    92341 aaaacaaatg aagatacacg gatcaacgtg ccaatcgatg acaacttcaa tactgtatta
    92401 aagagaattc gttcaggaga aaacgggatt ttcgaacgat tccgtgacaa tcttcttatt
    92461 gaaatcgtgt cttactggaa tgatcaacga ttgaaaaacg atgaaccaac tgcgcaagct
    92521 gttgcgacac cagttacaga agaaacagtc agtgtgccag aagaaaaaac ggaaaaacca
    92581 aaagcggaca caactgaaaa tacacgctca actgatacga atgccgcttt aagttttgct
    92641 gatttttcaa aagccgttgc tgagtatcct aaattttaca cagaacaaaa tgaacatgaa
    92701 gtagttatta atgaaaaaaa tgctgatgaa cctcgtaaat tagcaactat ttctatgact
    92761 actgaaaatg agttcgttat cgaaaaagca ctcgaacgga aatacaaagt caaattgact
    92821 ttgattcctt tgatcgaaca atttgcagcg acagcgatta ctgatcgata aaaattctat
    92881 ttgtcataaa aggctgggat ttaaaatccc agccttttta gtcatataat aatttctaca
    92941 gcaagtaaaa agaaattcct tctaacatca ggctcaaata tcagcttgac ctgtccaaca
    93001 aatcacctat gatcctacaa tgttaggcga cttctcttct acctgaagat atcttggagt
    93061 tgcctgattt cttaagatgc ctaaccttaa ctataaaaaa ttctaataat aaacatattt
    93121 tctgatagag aaaaaag
//
LOCUS       DAZKOW010000003        83108 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000003 DAZKOW010000000
VERSION     DAZKOW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.003"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(87..815)
                     /locus_tag="ACG912_000200"
     CDS             complement(87..815)
                     /locus_tag="ACG912_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFQ0511554.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            974..1855
                     /locus_tag="ACG912_000201"
     CDS             974..1855
                     /locus_tag="ACG912_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0511555.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(2092..3135)
                     /locus_tag="ACG912_000202"
     CDS             complement(2092..3135)
                     /locus_tag="ACG912_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0511556.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(3138..3806)
                     /locus_tag="ACG912_000203"
     CDS             complement(3138..3806)
                     /locus_tag="ACG912_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0511557.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERVGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            3948..6284
                     /locus_tag="ACG912_000204"
     CDS             3948..6284
                     /locus_tag="ACG912_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFQ0511558.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(6399..6710)
                     /locus_tag="ACG912_000205"
     CDS             complement(6399..6710)
                     /locus_tag="ACG912_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511559.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            6891..8366
                     /locus_tag="ACG912_000206"
     CDS             6891..8366
                     /locus_tag="ACG912_000206"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFQ0511560.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            8413..8997
                     /locus_tag="ACG912_000207"
     CDS             8413..8997
                     /locus_tag="ACG912_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFQ0511561.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            8999..9418
                     /locus_tag="ACG912_000208"
     CDS             8999..9418
                     /locus_tag="ACG912_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFQ0511562.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(9699..9983)
                     /locus_tag="ACG912_000209"
     CDS             complement(9699..9983)
                     /locus_tag="ACG912_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFQ0511563.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(10335..11045)
                     /locus_tag="ACG912_000210"
     CDS             complement(10335..11045)
                     /locus_tag="ACG912_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFQ0511564.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(11042..12514)
                     /locus_tag="ACG912_000211"
     CDS             complement(11042..12514)
                     /locus_tag="ACG912_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0511565.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            12652..13125
                     /locus_tag="ACG912_000212"
     CDS             12652..13125
                     /locus_tag="ACG912_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0511566.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(13278..13829)
                     /locus_tag="ACG912_000213"
     CDS             complement(13278..13829)
                     /locus_tag="ACG912_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0511567.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            13934..14464
                     /locus_tag="ACG912_000214"
     CDS             13934..14464
                     /locus_tag="ACG912_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFQ0511568.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(14947..15390)
                     /locus_tag="ACG912_000215"
     CDS             complement(14947..15390)
                     /locus_tag="ACG912_000215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0511569.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(15406..16566)
                     /locus_tag="ACG912_000216"
     CDS             complement(15406..16566)
                     /locus_tag="ACG912_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0511570.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            17073..18773
                     /locus_tag="ACG912_000217"
     CDS             17073..18773
                     /locus_tag="ACG912_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511571.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(18875..19708)
                     /locus_tag="ACG912_000218"
     CDS             complement(18875..19708)
                     /locus_tag="ACG912_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFQ0511572.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(20012..22177)
                     /locus_tag="ACG912_000219"
     CDS             complement(20012..22177)
                     /locus_tag="ACG912_000219"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFQ0511573.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            22437..24731
                     /locus_tag="ACG912_000220"
     CDS             22437..24731
                     /locus_tag="ACG912_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFQ0511574.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            24724..25389
                     /gene="pgmB"
                     /locus_tag="ACG912_000221"
     CDS             24724..25389
                     /gene="pgmB"
                     /locus_tag="ACG912_000221"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0511575.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            25382..26389
                     /locus_tag="ACG912_000222"
     CDS             25382..26389
                     /locus_tag="ACG912_000222"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0511576.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            26411..27430
                     /locus_tag="ACG912_000223"
     CDS             26411..27430
                     /locus_tag="ACG912_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0511577.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(27711..29078)
                     /gene="nhaC"
                     /locus_tag="ACG912_000224"
     CDS             complement(27711..29078)
                     /gene="nhaC"
                     /locus_tag="ACG912_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0511578.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(29187..30608)
                     /gene="tyrP"
                     /locus_tag="ACG912_000225"
     CDS             complement(29187..30608)
                     /gene="tyrP"
                     /locus_tag="ACG912_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFQ0511579.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(30851..32728)
                     /gene="tdc"
                     /locus_tag="ACG912_000226"
     CDS             complement(30851..32728)
                     /gene="tdc"
                     /locus_tag="ACG912_000226"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0511580.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(33019..34275)
                     /gene="tyrS"
                     /locus_tag="ACG912_000227"
     CDS             complement(33019..34275)
                     /gene="tyrS"
                     /locus_tag="ACG912_000227"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFQ0511581.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(34387..34592)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            34855..36156
                     /locus_tag="ACG912_000228"
     CDS             34855..36156
                     /locus_tag="ACG912_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFQ0511582.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(36225..36824)
                     /locus_tag="ACG912_000229"
     CDS             complement(36225..36824)
                     /locus_tag="ACG912_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0511583.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            37078..37959
                     /locus_tag="ACG912_000230"
     CDS             37078..37959
                     /locus_tag="ACG912_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0511584.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            37972..39582
                     /locus_tag="ACG912_000231"
     CDS             37972..39582
                     /locus_tag="ACG912_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0511585.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(39659..40318)
                     /locus_tag="ACG912_000232"
     CDS             complement(39659..40318)
                     /locus_tag="ACG912_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0511586.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(40601..41266)
                     /locus_tag="ACG912_000233"
     CDS             complement(40601..41266)
                     /locus_tag="ACG912_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFQ0511587.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(41413..42018)
                     /gene="dhaL"
                     /locus_tag="ACG912_000234"
     CDS             complement(41413..42018)
                     /gene="dhaL"
                     /locus_tag="ACG912_000234"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFQ0511588.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(42046..43056)
                     /gene="dhaK"
                     /locus_tag="ACG912_000235"
     CDS             complement(42046..43056)
                     /gene="dhaK"
                     /locus_tag="ACG912_000235"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFQ0511589.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(43031..43426)
                     /gene="dhaM"
                     /locus_tag="ACG912_000236"
     CDS             complement(43031..43426)
                     /gene="dhaM"
                     /locus_tag="ACG912_000236"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFQ0511590.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            43580..43900
                     /locus_tag="ACG912_000237"
     CDS             43580..43900
                     /locus_tag="ACG912_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFQ0511591.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(44012..44869)
                     /locus_tag="ACG912_000238"
     CDS             complement(44012..44869)
                     /locus_tag="ACG912_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0511592.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(44977..46281)
                     /locus_tag="ACG912_000239"
     CDS             complement(44977..46281)
                     /locus_tag="ACG912_000239"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0511593.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(46278..46787)
                     /locus_tag="ACG912_000240"
     CDS             complement(46278..46787)
                     /locus_tag="ACG912_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511594.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(46962..49097)
                     /locus_tag="ACG912_000241"
     CDS             complement(46962..49097)
                     /locus_tag="ACG912_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFQ0511595.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(49180..50835)
                     /locus_tag="ACG912_000242"
     CDS             complement(49180..50835)
                     /locus_tag="ACG912_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0511596.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(50837..51151)
                     /locus_tag="ACG912_000243"
     CDS             complement(50837..51151)
                     /locus_tag="ACG912_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0511597.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            51321..51581
                     /locus_tag="ACG912_000244"
     CDS             51321..51581
                     /locus_tag="ACG912_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFQ0511598.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            51595..51909
                     /locus_tag="ACG912_000245"
     CDS             51595..51909
                     /locus_tag="ACG912_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFQ0511599.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(52639..53193)
                     /locus_tag="ACG912_000246"
     CDS             complement(52639..53193)
                     /locus_tag="ACG912_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFQ0511600.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(53319..55248)
                     /locus_tag="ACG912_000247"
                     /pseudo
     CDS             complement(53319..55248)
                     /locus_tag="ACG912_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(55554..56270)
                     /locus_tag="ACG912_000248"
     CDS             complement(55554..56270)
                     /locus_tag="ACG912_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0511601.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(56271..58094)
                     /gene="glpO"
                     /locus_tag="ACG912_000249"
     CDS             complement(56271..58094)
                     /gene="glpO"
                     /locus_tag="ACG912_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFQ0511602.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(58108..59604)
                     /gene="glpK"
                     /locus_tag="ACG912_000250"
     CDS             complement(58108..59604)
                     /gene="glpK"
                     /locus_tag="ACG912_000250"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFQ0511603.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(59740..61200)
                     /locus_tag="ACG912_000251"
     CDS             complement(59740..61200)
                     /locus_tag="ACG912_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0511604.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(61190..62485)
                     /locus_tag="ACG912_000252"
     CDS             complement(61190..62485)
                     /locus_tag="ACG912_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0511605.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(62817..63494)
                     /locus_tag="ACG912_000253"
     CDS             complement(62817..63494)
                     /locus_tag="ACG912_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFQ0511606.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(63524..64615)
                     /locus_tag="ACG912_000254"
     CDS             complement(63524..64615)
                     /locus_tag="ACG912_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFQ0511607.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(64716..65150)
                     /locus_tag="ACG912_000255"
     CDS             complement(64716..65150)
                     /locus_tag="ACG912_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0511608.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(65734..67191)
                     /locus_tag="ACG912_000256"
     CDS             complement(65734..67191)
                     /locus_tag="ACG912_000256"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0511609.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(67188..69083)
                     /locus_tag="ACG912_000257"
     CDS             complement(67188..69083)
                     /locus_tag="ACG912_000257"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0511610.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(69141..70916)
                     /locus_tag="ACG912_000258"
     CDS             complement(69141..70916)
                     /locus_tag="ACG912_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0511611.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(70966..72462)
                     /locus_tag="ACG912_000259"
     CDS             complement(70966..72462)
                     /locus_tag="ACG912_000259"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0511612.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(72656..74278)
                     /locus_tag="ACG912_000260"
     CDS             complement(72656..74278)
                     /locus_tag="ACG912_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0511613.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(74606..75919)
                     /locus_tag="ACG912_000261"
     CDS             complement(74606..75919)
                     /locus_tag="ACG912_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0511614.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(76164..76388)
                     /locus_tag="ACG912_000262"
     CDS             complement(76164..76388)
                     /locus_tag="ACG912_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511615.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(76408..76830)
                     /locus_tag="ACG912_000263"
     CDS             complement(76408..76830)
                     /locus_tag="ACG912_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0511616.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(76972..77682)
                     /locus_tag="ACG912_000264"
     CDS             complement(76972..77682)
                     /locus_tag="ACG912_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFQ0511617.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(77700..78197)
                     /locus_tag="ACG912_000265"
     CDS             complement(77700..78197)
                     /locus_tag="ACG912_000265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0511618.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(78234..78686)
                     /locus_tag="ACG912_000266"
     CDS             complement(78234..78686)
                     /locus_tag="ACG912_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0511619.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(78821..79426)
                     /locus_tag="ACG912_000267"
     CDS             complement(78821..79426)
                     /locus_tag="ACG912_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0511620.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(79876..80496)
                     /locus_tag="ACG912_000268"
     CDS             complement(79876..80496)
                     /locus_tag="ACG912_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0511621.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(80498..81367)
                     /locus_tag="ACG912_000269"
     CDS             complement(80498..81367)
                     /locus_tag="ACG912_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0511622.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(81390..81797)
                     /locus_tag="ACG912_000270"
     CDS             complement(81390..81797)
                     /locus_tag="ACG912_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFQ0511623.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(81816..83105)
                     /locus_tag="ACG912_000271"
     CDS             complement(81816..83105)
                     /locus_tag="ACG912_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0511624.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        24361 a        17414 c        14530 g        26803 t
ORIGIN      
        1 caaaatcagt ttgtcaaata caaaaaaact gactaaaaga tttgatcctt tttagtcagc
       61 aaaaaatggc ttctctttga tttttattat aacagtggca taatccaatt tgtccacatc
      121 cagctcagtg gcatgataac gatcattgca ggtatcatgt cagctactgg gaacattttc
      181 acgttgatca ttctaaaacc ggtagcgacc atcaagaatc cgccgcaagc tttaaaatct
      241 aagatcatgt ctggtgtagt caatggataa ataaattttg ccaataagaa caaaatataa
      301 aaaataatga attggggaat tgctatcaca gaaacgacat accctaaatt acacgcaaaa
      361 atagctgctg tgaaaaaatc aaggacagat ttagaaatca aaatcgtact gtctcctgtc
      421 atcccagaat ctaaacttcc atagatcccc gtaccacttg cacaaaatag aacaatgacc
      481 gtcactaatg tgctgataaa ttcatcgtgg gtcataccaa gcttttcact agggaagatt
      541 ttagaaatag gtacttgcat cagcgcagcc cccttattta tcccattacc taaatgaaca
      601 atcaaaccaa tcgctgttcc tatcactaaa gcaaaaataa cagccggcat atttttcatc
      661 ggcgcaatgg aatagattcc catccccatc gaacacacac caaaaaccat cgtcagctgt
      721 gctttaaaat gttcacttaa tttatttcct actaatccgc caattagtcc gccaaacaaa
      781 acggaaccta cattgatcaa aactccagtt ggcatcaaaa aactcctctc tttttctacg
      841 aagcgaaaaa aatcagaaaa atttctgtaa ttattgcttt cgctgcaaga aagattgtaa
      901 cagacagaga agattacacc aattcatttt ttgtgttaaa gtattcttta ttggaataag
      961 taggaggaat atgatggata tccataaatt cgaagttttt cttgatttag ccgaaacaat
     1021 gaatttcacc aaaactgctg atcgacaatt caccacccaa ggaaatattt ctaaacagat
     1081 tctttcgtta gaaacagaat tagacgtcaa attattcgaa cgcgcccata gaaaaatcga
     1141 attgaccgaa gctggaagct tattgctccc atatgtaaaa aatgtcgtag aacaatacca
     1201 tgctatgcaa gaagtgcttg aaacctatac gaaagacaac aatctttcat taaatatctt
     1261 aacgataccg acaatgatca attataaagg tttttccaaa atcaccgagt ttttgaaacg
     1321 ccatcctgaa tttacagtcc agttgaaaga agtggaaagt gtcgaattaa cttacaatga
     1381 cgccaatgaa catagtgaca cgatccactt tgctagaagt ttccaggccc caccaagttc
     1441 gattgagtgg ttgcctactg aaacagacga ttttgtagca gttctaccta aaaatcatcc
     1501 tttagctagt aaaaagaaat tggatctaag cgaactgaaa aaggagaact ttttgctcct
     1561 tggccccact acgaacttgt atcaacctgt actagatctg tgtcgcgaag ctggatttga
     1621 accaaaaatc tcttataaag gagcaagaat cgacttgatc atcaatatga ttgcaaatga
     1681 catggggatt gctattgtta tggaaaaaac agtcaagaat ctattaaaag aaaatactgt
     1741 gattctccct atttctccaa caacagagag ttatcttgca tttactcgta aagtcggtga
     1801 gcattctttg gcttcagata cattttggga gtatttaaat caagaagaaa actaagccct
     1861 acattttctg tccatctatc cacccaaaaa aagagagcga cgcatgactt tcgctctctt
     1921 tcttgcatca tgtccttgtt tcaatgacac ctctaacatt tctcgtagtt caataagaaa
     1981 gcagcgattt ttatgccaaa agaaccgtaa gaacatttcc ctaatcacaa aaaaacgact
     2041 aaaaacccct aaaaaccttc aatagaattt ttaggggttt tattcttttt cctattgttt
     2101 acttaaccgt atcattgctg tcgttcctac tccttcgata ctgctataat caatacgtcc
     2161 atcatgcagt tctagaattt ttttaaccag tgaaagtcct agcccattcc cagaaatttg
     2221 acgacttgta tctccttgat aaaatttttc aaacattcga tctctggtct cttcattcat
     2281 tcctacacct gaatcggtca cttccagtac aatctctgtg gctgtctcaa atagtttgat
     2341 atgaatctgt ccattgattt gattgtactt tatggcgtta tccatgatat tcagccacac
     2401 ttggtacaaa aactcttcat tgcctgtgta atttactgca gcaagttcga tatccaattc
     2461 tagctgttct ttctcccatt taggctgaaa aaaaacgatt acttctctga tttgttcatc
     2521 taatcgatac tctttctttt ccagttggat acgctggttt tccagtttgt tcaattttaa
     2581 gatgttctct gttaattgtg aaagctgcgt gatactttcg ataatccgat ataaaaatat
     2641 ttggcgttct tcgtcagaga gatttggggc ttgtaacaat tgaacatatc cttggattgt
     2701 tgctagtggt gttttgaatt catgggatac actggacaca aagtcattcc tcagtgtctc
     2761 gatactattg agttcttgga ccataacgtt gaaatcttta tacagttgct gtacttctgc
     2821 tactttctgc tgttcatcca ttcgaatcga aaaatctccc gttgctacca gactcatatt
     2881 tgtccctagc gcactgattg gatgcaggat acgccgccca acaaaaatcg atattcctgt
     2941 acccacgacc atactgaacc ccgcaaagac gaataacggg tataacgttg gttttccagc
     3001 cgattcagca ggcgagatag aatgtcgaga caataagaaa ataaaaccga gaaaacacag
     3061 cagtgtcact aagatcgaaa tgaagacaat actagcaaaa tagatccata attgtgaaaa
     3121 tagctttttt ttcatcttta aataaccgcc ttatagccta gtccccgtac tgtgatgatt
     3181 tgaaaatctt ccgtcttttc aaaacgggtc cttaaacgtt tgatatgtac atcgatcgtt
     3241 cgttcttctg tattcgtatc taatccccaa atatcatcca tcaactgttg gcgagtgaag
     3301 atcttgttgg gataagacag taatttatac agaagcagaa attctttttg aggcagaatc
     3361 aacgaatgct gatcttgtaa cactgtataa gcatctagct ctaattttgt ttgtccgatt
     3421 atcaagctac tgctgtaatt gatctttgaa cgtcgaagca atgcttccac acgtaggatc
     3481 atctcattga catcgatggg ttttgtcata taatcatctg ctcctaattt gaacccgcgt
     3541 ttcttatcct caaaatcggc ctttgccgta atcattagaa caggtgtgtc aaatctggtg
     3601 ctacgtaatg agtctagcaa gtcatagcca tccattttcg gcatcatgat atctgtaatg
     3661 attaattcta tacgtgtcgc ttctagtttt ttcagcgcat cctctccatt caatgcggtg
     3721 atgactgaaa agcccactcg ttccaacaca gaacggtaaa gcaactgtaa ttgcggatca
     3781 tcttctacta ctaaaatacg attcattgat ctccctctgt tttttgaaaa atttctagat
     3841 tttttcttgt agttcatctt cagttaacat ttatttcgta tactacgatc attgttctga
     3901 tatacttatt agtataccga atatttcaat acgggagaag ggtgattatg cttcaattaa
     3961 aagatataaa aaaatattat aaagtcggcg aaaccaccac caaagcattg gatggtgtgt
     4021 ctgtcgcttt cagacaaaaa gaattcgtag cgattttagg tcctagtggt tctggtaaaa
     4081 cgactatgct gaatgtgatt ggtggactag ataattatga ttccggtgat atggtaatca
     4141 acggaaagtc tactaaagac tttaaggaca gtgactggga tgcctaccga aacaactcta
     4201 tcggtttcat ttttcaaagt tacaacttga ttggtcatct aggcattatt gaaaacgttg
     4261 aacttgggat gacattaagt ggtgtttcta aagacgaaaa acgaaaacgg gcagaagatg
     4321 cattgcaccg agtcggcttg acaaatcata tgcataaaaa gccaaatcag ttatctggtg
     4381 gacaaatgca gcgagtggcc atcgctcgag cactagccaa tgacccagat atccttttgt
     4441 gcgatgaacc aaccggagcg ttagatacag aaacaagtat ccagatcatg gaattgatcc
     4501 aagagttatc gaaagaaaaa ttagtgatca tggtcacaca taatcctgaa ctggcccatc
     4561 aatacgcaga tcgaatcatt gaattttctg atggtaagat cttaacagat tcccatcctc
     4621 acatcgaacg accaaaagat gatcaattta acttacgcag aacaaagatg agtttttgga
     4681 cagcgttaaa gctttccttc aacaatatcc gaacaaaaaa aggacgtact ttcttgacct
     4741 cctttgcttc cagtattggg atcatcggaa tcgcgatcgt tctgtccctt tcttctggat
     4801 tccaaaaaca aatcgataac acgcaagctg aaacgatggc gaaatttccg attactattt
     4861 ctaaggtaac gactaaccag actcgtgacg atgccggatt gggtgcaagc aaagcggatt
     4921 atccagacag caaaacgatt actgcaaaag tcagcgaaga agataaagca cagcacacga
     4981 ataagattga tcaaacatac gtcgattacg taacagacat cgatcctaac ttaagtaata
     5041 atatcggatt tacccgtact acaggaatca atcttttacg tgatgtaaat ggcaaagtac
     5101 agcctgtcag cttctcgaat caaaatcctg atacggaatc tctttcttta tctagtacga
     5161 tgtcagcaat gacaggtgtg ggagtgtcaa gtttccctac acagcttgac actagcaaag
     5221 aaaacttttt aaaagacaat tattctttac ttgccggaag ttatccagcc tcagcaacag
     5281 atgtcgtgct gatcgtagac ggcaataata atactaatat caacgcattg aagaatcttg
     5341 gatttgatgt gaaagaagac gaaaaacttg attttgatga tatcgttggt acaacattca
     5401 aattagtaaa caacaatact tactatacaa aacttccaac tgggaatttt atccctaata
     5461 cagattatga tgcgatgtat cagaatgctt ctgatgaatt gaagatctcg gggatcctac
     5521 gcgtgaaatc ttcttctaca atgaatctct tatctccagg aatcgcttat agcgatcaat
     5581 taacgactca aatcgtcaac gaaaacaaag aatcagaaat cgtcaaagct caaagagaca
     5641 gtggtgtaaa tgtcttaact actgaaaaag ttgacgaaaa tgccaaacaa acattgctta
     5701 gttatttagg tggagacagc ttgccttcaa gtatcatgat ttatccgaac aattttgaag
     5761 acaaagaaaa gatcttggat tatctagatg attacaataa aggcaaatca gatgaagata
     5821 aaatcattta tactgattta gctggaacga tgactgaact gactggcgga ttgatggatg
     5881 ctatcactta tgtccttatt gcttttgcag ggatctcttt agtaacaagt atgatcatga
     5941 tcagtatcat tacttataca tctgtgattg aacggacaaa agaaatcggt gtactaaaag
     6001 ctttaggtgc acgtaaaaaa gacatcactc gcgtatttga cgcagaaacg tgtattttag
     6061 gtatttcttc tgggatatta ggtgtgttta ttgcatggct agctacgttc cctatcaata
     6121 gcatcttata caacatgacg gatctaaaaa atgttgctca actaaatcct gtccatgcga
     6181 ttattctagt gatcgtctct actatattga cgatgcttgg cggtcatctt cctgcacgaa
     6241 tggcagcgaa aaaagacgcc gctatcgctt tgcgtgcaga ataaggttgt gacaaccaca
     6301 tttagatttg agtcataaga gcgctgaaat agtagaaaac agcaagaaac gaggaagtaa
     6361 tatgttcctt gtttcgcgct gtttttttta attatttttt ataattctgg tatacttttc
     6421 tttcctttgt accacaactc ttctggcgta tacctgtctt ttccatttat gaatactgtt
     6481 ccatctgctt caatcgttac ttcagtccca atccacatat tacgagaaag tgcgagaaga
     6541 ttttttcttt ttcctcttcc tttttttcca aattgcaagg catcggttat ctcttctgtc
     6601 caatgagatt tcacgtattt tacttctccg ttttcaaatg taactttcaa aatattcgct
     6661 atattttcca cttttactgc tttaggaaat gactcctcca tttttttcac tttccttcct
     6721 ccaacactaa gtatgcgctt tcttttagtg ataagtatat catagtttat tctattatca
     6781 aaaaaacgca tccattccat tgtagaatac tttaccgaac aagttgaatt gttttcctct
     6841 ttttcacctt ttactataga taggtaaaga caactaagga ggaatctctc atgacagatc
     6901 aaccgtatga tttaagaaaa gtcatcgaag aactaaaaac acttccaggt caatatcacg
     6961 aaacagatat tgaaatcgat cctgaagcag aattatctgg tgtttaccgt tatatcggtg
     7021 ctggcggtac agtaaaacgt ccgactcaag aaggaccagc tatgactttc aacaatatca
     7081 aaggtttccc cggcacacgt gtgaacatcg gaacaatggc tagtcgtaaa cgtgtaggtg
     7141 aaattttgca ccatgattat cgaacacttg gtcgtttctt gaaagacgct gtagaaaatc
     7201 caatcaaacc tgtgaaagta gacaaagcag acgctccagc acaagaagtc gttcatctag
     7261 ctaccgatgc agacttcgat attcgcaaca ttttgcctgc acctacaaat actcctgaag
     7321 atgctggtcc atacatcaca atgggtgtag tcttaggatc agatccagaa aaaacaatga
     7381 gcgatgtaac gatccatcgt atggttctag aagataaaga tacgatcggt atgtacatca
     7441 tgcctggcgg acgccacatt ggtcacttcc aaaaacaatt cgaagccttg aataaaccaa
     7501 tgcctatcac tatcaatatt ggtttagatc cagcaattgc cattggcacg actttcgaac
     7561 cacctaccac tccacttggc tacaatgaac tatggtgtgc aggagctttg cgtaacgaac
     7621 ctattcaaat cgttgatggt atagcagtcg atgaagtggg tattgctcgt gctgaattca
     7681 tcattgaagc agaaatcatg ccaaatgaaa caatgcaaga agatatcaac acgaactcag
     7741 gaaaagcaat gccagaattt cctggctata ctggggatgc taatccagct gtacaagtag
     7801 tgaaagtcaa agcagttact caccgaaaag acaatccaat catgcaaaca acgattggtc
     7861 ccagtgaaga acacgtatct atggccggta ttcctacaga agcaagtatt cttgccttgg
     7921 tcgataaagc tattccagga aaagtagtaa atgtctacaa tcctcctgca ggtggtggta
     7981 aattgatgac catcatgcaa atccgtaaag aaaacgaagc ggatgaaggt attcagcgtc
     8041 aagcagctat tcttgcttta tcagccttca aagaattgaa gacagtcatt ttagtcgatg
     8101 aagatgttga tattttcgac atgaacgatg tagtttggac tttcaacact cgtttccaag
     8161 gagataaaga tatcatcgtg atcccaggaa tgaggaacca tccattggac ccatcagaat
     8221 taccagaata taatcctggc tctattcgtt tccgtgggat aagtgcaaaa actatctttg
     8281 acggtacagt tcctttcgat atgaaagaac attttgtacg tgcaaaattc caagaagtag
     8341 aagattggga aaaatatttg aaataatgaa tgatctgcaa ctgttttgac atcataaaaa
     8401 ggagtgcgga aaatggaaca attggcacaa cggaagaaaa aaatcgtgat tggtgtaagt
     8461 ggcgcttcag gcaccattta tgccatcaac ctagtgaaga aactaaaaga atatccgatg
     8521 atagaaacac acggaatcat cagtccatgg gcaaaacaga acttaaagct ggaatccgac
     8581 ttatcactaa ctgaattcga aagcctgttt gattaccatt attcaaacaa agatttaggt
     8641 gctgctgtcg ccagcggatc atttttgacc gatggaatga tcatcgtacc ggcaagtatg
     8701 aaaacagtcg caggtatctc tgttggttta ggcgacaacc tgattgcacg tgctgctgat
     8761 gtaacaatga aggagcaacg aaagttagtc attgttcctc gtgaaacgcc attaaatgcc
     8821 attcacttgg aaaacttgac taaattagca cgactaggtg tgcaaatcat accaccaatt
     8881 cctgcttttt ataatcagcc tcaaacgatc caagatcttg ttaatcacca tacaatgaaa
     8941 cttttggatc aatttgaaat tgtaaatgag cattcaaaac gttggcaggg tgattaatat
     9001 ggaaaaagaa acgatagttg aagtaaccaa actaggtttc acgatccaag ctgctgttaa
     9061 agtaatcggt aaagacttat tgatcacttt aacaggcgga gatacaccac atatcggaac
     9121 agtcactaca ttttctcgtg atacagagat gcaaactgta cgtttcccta gccacgatgg
     9181 tcggttccat aaagacgatg tcttagccga aaaaatagct gtgatcatca aaactgccgt
     9241 tccgggaagt tgtacgatca cttccggtat ccatgttgac catatctcca atgcacaaat
     9301 caacgcctct tttccgatgt cagaagagtt aggttacaag attttagatt ggttggaaaa
     9361 aatagatttc catatcaaag aaccagttta ttataaagat ggtgaaaaac cattatgaaa
     9421 gaagaggttg tgaaatcagc gttatgacct gagtatgaga gaaaaaaatg gaaaaatagc
     9481 ttctcatatt ttgccgtttt tgagctaata ccgagggaca gctgattgaa cgccgtttat
     9541 caagaggttg tgataaagcc gcttcgtttc gagtagtaag aaaaattttg taaacatggg
     9601 tttccacatt tttgcataaa tttgacttac taccgagaaa ctggcttttg aacaccgttt
     9661 atcaagaggt tgtgacacgt cttttgtcac aacctctttt atttttgctt cactgtaact
     9721 gctacaatat caccgatttc ttttccgatg tttttacgaa tatcttttcg tagtccaagg
     9781 atgtagcaga ctgaaccatc tggatttttc acgcccatat tcacgatact gccttcatac
     9841 ggcacattgt caaaagttgc ttggacttta actcttccag caccgaattc ttttcttata
     9901 tcatacggaa aaataatata tgccccgccc ttgccgactt cggaagcata gatttttgat
     9961 tcaaaagaat aaatttccct cattttattt ctcctatttt ttacatctag tatagcatag
    10021 caaaaaaaag aaggaactgt gacaaaagtc ttgtcgcagt tccttttttc gaataaacgg
    10081 tgttctcctg ccaactcctc ggtcacaagt cacaatagtc aacaaacatg agaagctgtt
    10141 ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct cttccttaaa
    10201 cggtgttcaa aagcttgcat ctacggcact aagcccaatc agatgaaaaa tatggagtgc
    10261 tatttttatc tgattgttct tactgcttga tgcagagcag ctttttcaca acctcctaca
    10321 tggtgttcaa aatattattc gccttttgct tctttcacta attttgcgaa tgcttgttgt
    10381 tggaattcag cagctaaggc tttcgtacgt tgttcttgct ctgctttgct catttttgga
    10441 ttttcagtca ttgcttttac cataccgtct tgaacataag cttttgcttc actttccatc
    10501 gtactttgct gtttcttcac taattcagct aacttggctg cttggtccgt acttaattct
    10561 acccatgtct ctgggatttt gaatgtattc actcggctgt catattcgtg attgttatca
    10621 gcaatattga accagaattt atacatgtcg tttttgtatt cccaataagt agttttcgtc
    10681 actttttcag catcgccgtc tgtcttttct actttatttg tttcgttatc cgtaccagtt
    10741 gcttttggtt tttcttgttt ttcatttgtt cggtaaagat acactttttc ttttccattc
    10801 cctaaagctt tatacaaaag catatctgct ccttgggaat cagcactgga aacaattttt
    10861 tctgtttttg tttccgttac tttttccatt ccaaagtgat tggacaaatt agcgacgatc
    10921 aaacttagtg aagcgacgaa gattagtccg aaaatcaaag ataaaaaggt ttgccatgtt
    10981 tttttagcga acacgttcgt taaagcaaaa gcaagcaagc tgacaatcaa aactccaata
    11041 atcatgcatt atcccccgct ttcattagaa caggttcttt tttcttcaat aaaaaggcaa
    11101 taataaagcc aagaatccca aagatgatcg ctacgataaa cgcggcatgg taacctgaca
    11161 gtgtggcatt gattgcctga tctttataag ctaatggcgt attatccaat acagatttgc
    11221 ttgggatatt tgcttttgtc acattcgtca acacactgat caaaatagct gtaccaatcg
    11281 agcttgcgat ttgacggaat gtattattta ccgctgtccc atgactgatc aagttatttg
    11341 gcaatgcatt cataccagaa gtcgttaccg gcatcatgac catggagatc ccaaacatac
    11401 ggatcgcata aaggatcacg atatataaaa ctggagtcgt ttttgtcaag aaagcaaatg
    11461 gtgcagttgc cgctgtcaaa atgaacatcc ctgtgatggc taaacgtttc gcgccgtatt
    11521 tatcgaaaat cgcacccgtc acaggcatca tagcccccat gatcaatgca cccggcaaca
    11581 acatcaagcc agaatggaat gctgattctc cacgaatatt ttgaatatac aatggtacga
    11641 ccatttctgc ccctaccatc gccatatttg ttacaccagc taaaacagct gcaatcgtga
    11701 atgtagatga tttgaataca cgaagttcca agaatggatg ttccaaatgt aattgacgcc
    11761 atgagaacaa ggcgatgaaa atcacaccaa caactaagaa accaattact ttcaagcttc
    11821 cccagccgtc atctcctaca ctagaaaatc cgtaaagtaa actaccgaat ccaatcgttg
    11881 ataaaatagc ggataaaata tcaatgctag gatttgacaa tggaataaca cttttcatta
    11941 ggaagaatgc taaaaccaag acaagaacaa cgattggcag taccatacca aacaagtcac
    12001 gccaatggta gttatcaatg atataaccag ataatgtagg gcctaaagca ggagcaagtc
    12061 caataacgat ccctgacatc cccattgctg atcctctttt ttctggcggg aaaatagata
    12121 gcatgatatt ttgcaataat ggcatcgata ctcccacacc gcaagcttgg atcaaacgac
    12181 cgattagtaa tgtactaaaa ttaggtgctg taaagcaagt aatcgttccg atcaaaaaga
    12241 tcgtcatggc agaaagatat aaatttcttg aactgaattt attgatcagc catgctgtta
    12301 ttgggatcat gatcccgttg actagcaaga aaccagtcgt taaccattgc acatcagacg
    12361 ctgaaatatc gaaagctttc atcaatgtag gaaaagctgt cgtcaataag gtctgattca
    12421 aaacggtaca aaatgtacca attaataaga caacaactaa aagtgtccga ttatatggtt
    12481 ttccgtaaat atctacgggt tggttgttac tcatatttct ccttctttct gaattttttc
    12541 tgaaactact agaatacttt agtcctaaat tcgctatttg tcaacctact tgacacataa
    12601 gttaatacgg atataattaa gggaagcaag aggacaaaag gagtgacaaa aatggttggg
    12661 gcttccctta aacgtttgtt ggaaaatgat caattggttg ttggcatcag tgaattaagt
    12721 gatatcgtag gtgtgtctcc acgtcaatta aggtattggg aacaaaaggg attcattcgt
    12781 tcgatcgaag ctgatgcgaa ttgtcctaga aaatatcgtt tacctacagt cataaaagta
    12841 gaactgatca aaaaatattt agatgacgga tttactttgt caaaagctgc agaaaaggca
    12901 gaaatccgcc aaaagaaaat gcaccatgtc cgtaaagttt tttcaaaagc aatcaaagat
    12961 atcgaattga tcgacgaacg ctatacgatt ttacaaatcg gtaacttcaa agaagagaat
    13021 gctaagatgt acatcattca tgacaacgag acagacgaat taacttacaa gctccttcct
    13081 gtcgatgaaa aagctgacta cgaacagttg ttaaaagaac tctaaacgcc actaatctcc
    13141 taagtaccaa gctttttaat aagtagatgc tacataaata cttgaaaaaa ggcctgtttg
    13201 aacataagaa tatccgaacg gtgatctgcg tttctttcat atagaaagga attctccatc
    13261 actgttcgga tattttttta tttttttcgg ttactttctt ctaatacatc tgttagccaa
    13321 tttgcatatt ctgtttcccg attgatcgca tgcgctaaaa tcaggtagtg tccgtaattt
    13381 tcatgttttt ctttttcagt aggaaagatc gttttttttc gctctaatag atgaagtcgt
    13441 ttttcttcat gaaggtcacg ctgctgctga ataatttcta ctaatgccgg atcattcacg
    13501 tctttgacga aatacaattt cagaacaaat tcgtctcgat taaccggtag ttcattggtg
    13561 ggtgtatgaa tccactctgc caattgttcc ttcccttctt ctgttaattc ataaactttc
    13621 ttttccaatt tttcacctgt gatctctact tgatgctgaa tgattccttg ctcttccatt
    13681 tttgcaagtt caggataaat ctgactatgt tttgcttgcc aaaattctcc tatttcattt
    13741 ttgaacgatt gattcaattc atatcctgtc atcttcttct tacttaataa ccctaacaaa
    13801 atatatcgaa gtgtattttt tcttgccatc agttttccct ccttgacaaa tctttaaatc
    13861 cattctatca taaatgagta atcgaaaaca tgtagtttat tacatgtttt aatttataag
    13921 gagatgctta aacatgaaag aatttaaaga attggaagat tttattggaa ctcactttat
    13981 ctatacgtat gacaatggtt gggaatatga atggtatgcc aaaaatgacc atactgttga
    14041 ttaccgtatc catggcggaa tggtcaaagg ccgttgggta aaagaccaag aagctgatat
    14101 cgttaaattg acggacggcg tattcaaaat cacttggaca gagccaacag ggacagacgt
    14161 ggcattggac tttatgccaa atgaaaagaa acttcacgga atgatctttt tccctaaatg
    14221 ggttgaagaa catccagaaa tcactgttac tttccaaaac gaacatatcg cagaaatgga
    14281 agcagctcgt gaaaaatatg aaacttatcc aaaattactt gttcctgaat ttgctacgat
    14341 cacttatatg ggtgatgcag gtatagacaa tgaagatgtt attagcgaag aaccatacga
    14401 tggaatgcca gatgatatcc gtagcggaaa atacttcgat gaaaattata aacgagtaaa
    14461 ataaggttgt gaaagagagg ttgtgaaatc agcgttctga cctgagtatt agagaaagaa
    14521 acggaaaaat agttcctcat gttttgccgt tttttgagct aataccgagg gtcagctgat
    14581 tgaacaccgt ttatcaagag gttgtgaaat cagcgttatg ctttgagtaa taagaacgct
    14641 agttagaaaa tagcttttca tattttctag gctagtgggc ttattaccga gaagcagccg
    14701 tttgaacacc gtttatcaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa
    14761 caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg agggtcagct
    14821 gattgaacac cgtttatcaa agattgtaac aactgcatat tcacacagtt aaattagtga
    14881 cttcaaaatg aaataatgct cccagcaaaa aattaggctg ggagcattat ttcgttattc
    14941 tgttcatcaa aaatcagcgt tttgattcca tttcatcaca agttcctcct catttactgc
    15001 ttcatctttt tgctgtgttt gaattgtaaa accttgtgaa agatagaaat gaatagctga
    15061 ttgatttttt tgatagactg tcaaagtcag tgttttattg gtttctttta tttttttcat
    15121 tagttgcgta ccgattcttt gttgccggta ttcccttttc acaaagattc ctgcgataaa
    15181 ctgatcaatc accccagcaa aaccaataat ttcttgatct atttctgcta agtaaatcac
    15241 tgcatctggt aaagctttcc taacagattc tacttgattc tcccaataag ttgatggaat
    15301 gaattcatga gaatccaagt tagcatgcag ccaaatcgat agaacagatt ccagttcttt
    15361 ggcagacaat tttgttgttg tgcggatcat tttttctctc cttacttatt cattgatttt
    15421 aaaataaaaa ccattttttt gaagctcttt tacaagtttt cgcccctcaa aaaagtatat
    15481 cttctcttgt tttacttttc taataccaaa agaaacagca actttaggat catcagctga
    15541 aacgattcga taagttccac caggcaattg atccagcttg actttcccat gatggtcgct
    15601 ttgtactgaa aacggcacac catttctagt aagtgttttt ttcttatgtt tcaaaatgaa
    15661 tgaactacca tttaccggtt tccctgacga atcactaata ataaatgaaa aatcaagcac
    15721 ttttttactt aatctatact gatagattag tcgtccgata aacactaaca gtatgactac
    15781 agctattatc gttcctgcaa gaataaacca ttgccgcatg gttctttcct gatttccttt
    15841 tccttcttct ttttttaccg attcgttata aggaatccga tgtcctgtca ctagcaaacg
    15901 atgcgtgttg atcatatacg gcgtacatgt aagcaaggta actaagtctt ctccttctga
    15961 aatcgttaga acagacgtgt catctggcag taccacttgt atatttttaa cttgataagc
    16021 caatttctcc cccaaaacag tcaaaataaa cgtgtctccg ttttctaaac gatctaattc
    16081 ggtaaataac cgtcgattag gtaaaccgct gtgagcagag atcactgaat gcgtcgaatt
    16141 tccaccaagc gggaaagagg ttccttgcaa gacgcctgct ccattttcaa ggatcctacc
    16201 attgagccga tcataaagtg aaatatttaa ttggatctta ggtatattga tacttccgat
    16261 cagccattcc tctaattggc tggatgttgt actttcattt ctgttctttt cattgaaagg
    16321 atcagtacca ggattgaatc cttggtcagt taatcgttgg tttttctcct ccattttctt
    16381 tcgctgcact tcaatttcag ctgcggtccg tttttgtact tgttcgatac gtttttgatc
    16441 aataaaacta tttaaagaat ccacataaaa tggatataaa gcgatcagta accctacgac
    16501 gatacatatt aataaaaaca aacgaaacaa aagtcgacga gactgttttt gcttactctg
    16561 cttcacatgt gtcctccctt gcaagttttt ttgttctttc ttttattata tgggttcagg
    16621 aaaaaaatag aaaatgaaag ccgatgaggt tgtgaaaaag ctgtcctgca tcaagtaata
    16681 agtacagccc aaaaaaatag ctactcatat tttttgttgg gttgggctta ttaccgcaga
    16741 tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa actgcgttat gctttgagta
    16801 ataagagcgc tagtcagaaa atagcttctc atattttcta ggctagtgag cttattaccg
    16861 agaagcagct gattgaacac cgtttaccga gaggtcatga aaaagctact ttgtttcgag
    16921 tattaagaaa aattctgttt caaaacaagg agatgcaaca aaacttatgt cgcatcctct
    16981 ctccttagaa ctttcactta tgtttttctt gtaaaattca taaatatttt catgaatttt
    17041 ctaatttctt gcctacctct cgcataggat atatgatgaa tttatctaaa gtttgcaaag
    17101 gagttcgaca attgaaaaaa tatggcccat ttttagcaat ttttttcatt gctttggcat
    17161 ttctcatgcc tcatttattc acacataaac taattttggg tgctgatgcc ccattccatt
    17221 acaatcggtt ctatgatgcc gcgcaacaaa ttaaatatgg ggatttccat tattttcctt
    17281 ccctttatgg attccagcaa agtggcagga tcgtgaatgc cgtttacggc ccatttttcg
    17341 cttatttaca aggattgatc gtgttgcttg caggtagctg gtttcattat cagattcttt
    17401 caaatttcat tctttatctt atttcaggat tttctatgct gattttactg agatatgtca
    17461 atgttcgaaa ttgggtaagt gtaccagtag ccgttttatt catgagtacg tattctattc
    17521 aatactggtc tatcaaccaa ggattcacta gctgggggac ttgtttttat cctttatgta
    17581 tgatccctat tgctgatttc gtccttcata aaaagttccc tgttatcaaa gtcgctgtct
    17641 cagtgggact tatgacacag atccatttac tgtcttcgat catgttagct ttgatgtata
    17701 tcccttttta tctttattac ttttttaacc agactgaaaa aggaaaagca ctgcttcaac
    17761 tgattaagtc ggtcttgctt tattttgttc tgacagcaaa tatttgggca ggattagcta
    17821 ttgtttacag tgggaataag attcttcctc cctttgttaa cagagaaatg agtcaaaagg
    17881 cgatagatct ggatggcagt tattggctcc attaccctaa ggcctttcct atattgatat
    17941 tatcaggttt tttgcttttt attttctttc aacaaaagaa caccttattc caaaaaatcg
    18001 tgttgtacac aagtcttgtt ttcttttttc tttctactag cttgattcca tggacgacat
    18061 tgatcgaaaa aaatgtgcct tttattagtt tgatccagtt tccatttcga tttttcgtgc
    18121 catttacgct attgcttcta ctttaccttg ccctttcact gaaccagtgg tctgaacaaa
    18181 agtggttccg tcttttaagt atatttggca ttcttatttt ttcagcacag acgatccaga
    18241 acttatacca gcatctggaa aagtgggaaa atgaaacatt tgtcagtcga catacttatc
    18301 tattcgatac gccggaagaa gcaaggaaaa cattcttcag caaagatatg tctaccagct
    18361 tgttcgtttt ccaaaaaacc acacctgact atctgccaat ctataaggag acaaaagaca
    18421 ataaatacga ccggtataac gaagaaatac tagaaaacga aacaactttt ataaaaacac
    18481 atccaggcgg tcttttgaca atcaagtgga aaaacactgg ggacaaaact gttcatatcc
    18541 ctgttatcgt ctacgatcgg actgtgctac agcaaaatgg aaaaacattg actgattatg
    18601 aagtaacaga tatcgggacc ccaatcgtga agcagcaaaa aggaatcaac gagctcacgc
    18661 ttcattatca aacgccaatc tatttctatt tcattctttc tcttactttg attggctggt
    18721 ttaccttgct atgtttattt atttatcata gatacaagct tttacgtgct taggcaaaag
    18781 ggataaatac atccgatcaa aaaactctcc aaacggtata atcgtttgga gagttttatt
    18841 ttaatcagtt tttttatgtt atatgttata ttttttattc gttcacatct aattcgattt
    18901 ccaccccgta gtgatcactg atgattggac tatcgatccc atcaaaaatc cgttgatatt
    18961 ttttaacctg cattccttca gggacgaaaa taaaatctat acgcaatttt tctgtatttt
    19021 gttcccaacc atctattttc ttttccacag tagctgcttt tcctttttca ttcgcgacaa
    19081 catacgaatc ttgaagaaga aggcaacttt ctcttacagt ataataacct tgagagtcta
    19141 ctggattatt gaaatcacct aggaaaaaaa gtggtacgcg agtttccaaa aagtagtttt
    19201 ccaagttttt ccactcaaaa taaaagccac tttttttatc tgtccaccaa gaaaagtggc
    19261 agctcgagac aaaaatgtcc tgatcatcta tctttgtttt cccaatcaag atcttgcgtg
    19321 tctgataatc ttctttcttt tgaaattggg agacggtaag gacctgactt tccaatgttt
    19381 ttttcgtaag cagtgcattt ccttcttcat atttatcata accgatgtgg ctcatttccc
    19441 aactccaaaa atactctttt ccgtgttcct ttaaaagctg aactaaacgg taggcaaaat
    19501 tatctttacg gataggtact tgattcttta ccggacagaa cttcattagc ttcgcaggtt
    19561 ctacttcttc agattccagt aattggttga tttcttgtaa agcaatcact tcataatctt
    19621 ctaacaggat cttatctgct aattcttgca attttttctc tggttctggc tcaagccagc
    19681 tatgcgtgtt gagtgtcatg aatttcattg gttttctcct tgtcttcatt tcgaaacaaa
    19741 agggttgtga caaaattttt gtcacaacct ctccagcaaa tagtgttctc ctgctaactc
    19801 ctcagtaata agctcaatgg accaggagct attttttgtt tggctgtttt tatcacttgg
    19861 tgcagggcag ctttttcacg acctctccac taaacagtat tcaaactgct gcttctcggt
    19921 aataagccca accaaacgaa aaataggagg agctattttt gtttggctgt tcttatgact
    19981 tgatgcagga cagctttttc acaacctctc cttacatttt aatggagccg atcactttac
    20041 cagctgtcac tgtacctaat tgcttgatca ctacttcttc tactttatcg tttgtaaata
    20101 cgatcatgat cgttggatct ttttcttcgt tttggatcac atcaagatcc atctcagcta
    20161 atttcgttcc tgttcctact tcttggcctt ctttgacagc aatctcaaaa gctggctgat
    20221 tcatttggac tgtatcgatt cccatatgga tcaaaacttc gataccatct ttcgtttcca
    20281 gcccaattgc atgtttgcta gggaaaatac ttgtcacttt tccagcgatc ggcgcaacaa
    20341 cttctcttga tgttggaata acagcaaagc cgtcacccat cattttttgt gagaataccg
    20401 gatcttttac ttcagataaa ggaatcactt ctccagaagc tgcggaaact agttcttgtt
    20461 ctatacctaa gtattgctta ctacctgctt gtccatttcc tgtagaaacc atttctggct
    20521 ctggaatctc tgctcctgat tgcagcagat cttcgatatc tgatttcaag acatctgctt
    20581 ttggtccata aactgcttgg actccattgt ctttgacgat caatcccatc gcccctgctc
    20641 gtttccaggc ttcttcagtc cctacttttt ctcgatcatt tacacttacg cgtaaccgag
    20701 tcatacaagc atcgacatct ttgatatttt cttttcctcc cagcaaatga atgatctgga
    20761 ccacttgctg atctgctgtt cctgttccgc tgttagatac aacggtttct tcactgtcat
    20821 tgtcgtaatt tccgttacgt cccggtgtag caaagttgaa ttttttgatc aagaaatttg
    20881 ctaagaaata cgtaacaaca ccaaaagcga tcacacacaa cacgaagttg ataagatctc
    20941 cacctagacc cgctttgata gctaacggtg tccgagtcag caattcgata ttcccaaatg
    21001 aatggacccg caatggcaag atatctgcca tcgcaaatgc agccccttgg atcactgaat
    21061 aaatgacata taatggcaca gcagcaaaca taaacatgaa ctccaatggt tctgttacac
    21121 ctgttagaaa gacagccaac gctgctgaaa aatacattga tttatatctt gctttcttat
    21181 caggatcgac attacgatac atcgcaaaag ccattcccat caagatacct gaagaaccaa
    21241 tcatttgtcc tactttgaaa cgagccggcg tccagttatg caacacaaac tcatattgcg
    21301 acatatcccc tgcaccttta agattcacta aatcagtggc ccaagccaac cataacggat
    21361 cttgcccaaa tacttgtgtc cccgcctgtg cgcctgataa aatctcatac gatccgccaa
    21421 gctgtgtata gttgatagga atcgtcagca tatgatgcaa tccaaatggc aatagtaaac
    21481 gttctaatgt tccaaataaa aacggtgcta gaatcggtgc gctttcttgt gattctgcaa
    21541 tccataaacc aaaattattg attccagcct gtacgtttgg ccacaccaca gcaagaatca
    21601 atgccacgat tgttgaccat aagataacaa caaacggaac aaagcgcttt ccattgaaaa
    21661 atgacaaagc atccggcaat tttctataat tatagtactt attataagcc attgccccga
    21721 caaatccagc gatgatccct acgaatactc ccatattcaa agctggtgct tccaatacac
    21781 ttgtaaaaaa tcctttaacc atgatcttcg taccgaacag agtgtgggta aatgcatctt
    21841 cattcgcaag catgtcactt gtgacaccaa agatagcccc agtgatccga ttgattaaaa
    21901 taaatgagat cccagctgca aatgctccgc ctgctctttc ttttgcccaa cttcctccta
    21961 ttgctaaagc aaataaaata tgaagattac caataattgc ccaaccaata ttttcaatta
    22021 ctccgcccgt tgtgacaagc ggcgtccaat caggattgat caagggaatt gatttcccta
    22081 tactgatcat caacccagct gccggcataa ctgctaccac aaccattaaa gcctttccaa
    22141 atttttgcca aaactcaaaa ctaaatatct ttttcattcc gtttagctcc tttacgcaat
    22201 cggtttcgtt aaacgtatta tagcgtgcgt tttctttttt gtaaatgctt tattttttat
    22261 ttttctgtct ataccattct ataaaaccta taatacgtgt gttcccttct ataattttct
    22321 cctaaaaatt tattttttta agcttttttc tatgaaatat tttcttttgt ctattgactt
    22381 gtgcaatcgg ttgcgtttat tatgaacgta taaaacagct cattaggagg agaaaaatga
    22441 aacagatcaa acgactattt gctattgatc catggaaaat cagcacgaca caattagata
    22501 aaaaaaatct tcgactgcaa gaatctttaa caagtattgg aaacggttat atggggatgc
    22561 gcgggaactt tgaagaaact tactcagggg accaccatca aggtacttat ttggcgggtg
    22621 tctggtatcc cgataagaca cgtgtcggct ggtggaaaaa tggttatcca gaatactttg
    22681 gaaaagtgat caacgccttg aactttatcg cgatagatat ttcgatcaac gatcagctga
    22741 tcgacttggc ttctttggaa ccagaagact tttactggga attagatatg aaaaatggtg
    22801 tattatctcg tacattcaca atcactactt ctactaataa agtacgcatt tccttcgaac
    22861 gttttctgag cattgtcaaa aaagaatcag cttatatcta tttaactata gaaatgcttg
    22921 aaggtagcgg aacagtcaaa ctcgtatcaa aattggatgg aaatgttcaa aacgaagaca
    22981 gcaactatga tgaacatttt tggaatgaga ttgatcgtgg aactatagaa gaaatcggct
    23041 atttgacgac taaaactatt ccaaacaact ttgggattga acaatttact gttactgcag
    23101 caatgcgcca tttcgttaat ggaaaatcag tcatccctga ctatcacgaa gatactttag
    23161 cattacatgg ttgcttgtct gcagaattat ctcaaggtga gacatggtca gtggaaaaag
    23221 aaattatcgt tcttactagt agagatattc cagaagcgca gcaagcagaa aaagctgtct
    23281 cagaattaac gaaccagaca aacaattata tccaagcaaa agaagagcaa acagcggctt
    23341 gggcagaacg atggaagctt gccgatgtgg tcatcgaagg agacgaagaa gcacaacaag
    23401 gtattcgatt caatttgttc caactattct ctacttacta cggcgaagat gaacgattga
    23461 atatcggccc caaaggattc acaggagaaa aatacggcgg agccacttac tgggatacag
    23521 aagcgtatgc tgtccctctt tatcttgctt tagccaaacc tgaagtaact aagaatctgc
    23581 tgaaatatcg gcataatcaa ctgccgcaag caatccacaa cgctcagcaa caaggattaa
    23641 aaggcgcgct ttatcctatg gtcaccttca ctggtgtcga atgccataac gagtgggaaa
    23701 tcacattcga ggagattcac cgaaatggcg cgattgccta tgcaatctac aattatacga
    23761 attacaccgg cgatacggaa tatttgaaaa atgaaggact ggaagtttta gcagaaatcg
    23821 cacgcttctg ggcagaccgt gtccattttt caaaacgagc aaataaatac atgatccacg
    23881 gtgtgaccgg cccaaacgaa tatgagaaca atatcaataa caattggtat acaaatacga
    23941 tcgcagcttg ggtcttgcgt tacacgcgag aaaattattt aacttatcaa aaagaagctg
    24001 ctatcactat cagcgaagaa gaactagcca aatggaaaga tatcgcagag aacatgtact
    24061 tcccagttga cgaagaatta ggaatctttg tccagcatga cacattctta gataaagatc
    24121 tcatgcctgt atccgatatt cccacaagcg atcttcctct gaatcagcat tggtcttggg
    24181 acaaaatcct tagatcttgc ttcatcaagc aggcagatgt actacagggg atttatttct
    24241 tcaatgatac ttttacaaaa gaagaaaagc aaaaaaactt tgatttctat gaaccaatga
    24301 ctgtccatga gtcttccctt tcgccaagta tccattcgat tttagcagca gaattaggaa
    24361 tggaaagcaa agctgtagaa atgtaccagc gaacagctcg cttggatttg gataattaca
    24421 ataacgacac agaagacggc ttgcacatca cttccatgac tggtagctgg ttgtcgattg
    24481 tccaaggatt tgctcaaatg aaaactgccc atgatcagtt gagattcgca ccatttctgc
    24541 caaaaacttg gaaagcttat tctttccata tcaattatcg cggacgttta ttgttcatct
    24601 cagtcacaga tgaacaagtt actttcgagt tattagaagg ttctcctcta gaaatttacg
    24661 tctatgaaca gtcttattct ttagcggatc gtctagaaat aacgctaaga aaggaggaag
    24721 caaatgttta aaggggtatt gtttgattta gacggtgtca ttaccgatac tgctgaattc
    24781 cattatcgtg cttggcgaaa gcttggtcag gaaatcggca ttacgatcga ccacacattc
    24841 aacgaacagc taaaaggcgt cagccgagaa gactcactct ctttgctttt agcttatggc
    24901 ggaaaagaac actctttttc aaaggaagaa tttgcagaat tagcgaaaag aaaaaacgat
    24961 tattatttag agatgatcca gacgatcgaa ccaaaagatg tcttccccgg ggtcgtgcct
    25021 ttactggatt ctttaaaaga agaaaaaatc aagattgctc ttgcttctgc aagtaaaaac
    25081 gggccattcc tacttgaaaa aatgggtctt acaccttact ttgatgctat tgccgacccg
    25141 gcagaagcaa caaacggaaa acctgcgcca gatatcttcc tccttgcagc aaaagctgtc
    25201 ggactaaaag cagaagaatg tatcgggatc gaagacgcgc aagccggtat ccaagccatc
    25261 ctctcaagtg gcgctcaacc aattggcgtg ggacgtgctg aagaccttgg agaggagatc
    25321 ccgcttgtcc ctgatacgac ttttcttacg atcgattact tgaaagaaaa gtggcatgac
    25381 catggataat ccagagatct ccgtcgtacc agttccagag actgaaataa agaaaatcac
    25441 attgaagaac gactacttag aagtcgttct tctcaattac ggtgcccgcc tgcaccaaat
    25501 cttcgcacca gacaaagaag gaaaatcaga aaatgtttta ttgtcttacg atcgtttcga
    25561 agatgttcta acagacaaaa gcttttttgg agcaactgtc ggacctgtag ccggaaggat
    25621 ccgggatgct tcttggggtg agcatagatt ggagaaaaac tgcggatcac accatatcca
    25681 cggtggcacg aaaggctggt ctttccaatt ttgggaagta gaagttttca aaactccatc
    25741 atcaataggt gtggtctttt acttaaaaga tgaattttct acctatccag gaccaatcac
    25801 tgctacgatc acttaccgtt taacaaaaaa cgaattagag atgacaacct ctgctagtag
    25861 ccttgttgaa accatctgca atccgaccaa ccatgcctac ttcaaccttt caggaaatgg
    25921 aacaagagac atctatgaac atcagttaac ggtcttttta gatggtgtgc ttgaattaga
    25981 tcaagaaaaa ctgcctactg gaaattggac aaaaaaagaa aaactgccaa tcgactttag
    26041 aaaatcccca acactgcaag agatccttgc atgttatcca gatggactag atgatgtatt
    26101 tcttttgcat catcctaggc tttccaaaac ttccttacaa ttattcgaga agcattctgg
    26161 tcgccaaatg actatagcaa cttctaataa aagtatggta ttattttcta cgactggatt
    26221 cgaagctgat ttctctgtca atggaaaaca gatgcattca aactacggat tagcaatcga
    26281 accgcaagaa atccctgata tcgttcattt ccccaaattt ggatcgatca acctccatcc
    26341 tggtcaagaa cggatcagtc agacgattta tcggttctct gcacaataac aagagaaagg
    26401 tgtttttatc atggctatta ccgtaaaaga tgtggcgaaa aaagccggtg ttgccacctc
    26461 gaccgtctct cgtgtcatca acgatcatcc tagtatttcc gagacaacaa agaagaaagt
    26521 ccgaaaaatt atggatgaac taggttatgt tcctaacatg actgcccgca atctaggaaa
    26581 acgcatttcc agtgccatcg gcgtaatctt accccctctt gattcaaaag agcggatcgg
    26641 taacccattt tatcttgaga tcatggaagc aatcaacgaa gaagcgcgga attatgatat
    26701 gactacagca atcgctactg ctaaaaattt tgaattgctg ttagagaacg tccagcgtat
    26761 gcatttacaa aaacaagtag atggttttat tctagtgtat tcagatagtc aagatactgt
    26821 cattgattat ttataccaaa accacatccc tttcactttg atcggacagc cttatcatca
    26881 tgagaacgaa atcgtttatg tagataatga taaccagctt ctaggtaaac aagcaacaga
    26941 atttttgatc cagaatggac ataagaatat cctgtttgtg acaaacacga ctcatgaaag
    27001 tttgtatttc gaacgatatt tcggttatca aaaagcaatg atgatggctg gtcttcctgc
    27061 gttatccagt gtagtactag agcatccgga ggattatctg tcctttgaga aaacgttgaa
    27121 agaaaacgaa gccacagcat tggtggtcat tgacgatatc ttcgcccttc gcacgatgca
    27181 gttggcgcaa atgtatggct atgtcattcc agagacactc tccgtcatta gcttcaacaa
    27241 ctcgatcttc tcaacattaa cacaccctta ccttacaagt atcgatatcg atatctccga
    27301 attaggacgt gtcgctatgc aaaagctgcg cgaattgatc catgaagaat tgagcagtgg
    27361 cgtacgtttg gtcatccctc atcgcttgat caaaagagag acagtcgttc ctttagaaaa
    27421 cccatactaa atagctgact cactaaatac gctgaccatt tatcctatgg tcagcttttt
    27481 atgtaaaaga agatactact tctaacacat tgtttatctg taaaaaagag cgtgggacaa
    27541 aagtaaagaa cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagcaa
    27601 ctccttcgga aataagctga aattcacaaa aatttaaaaa gcaattttct tgagtttctt
    27661 cttatttctt ggagttaaac gcttctgtcc cagcctcttt tttccttcct tcaatttttt
    27721 acacgtcgac gattcaataa aaaaccgcct aatattgtaa agacaggtgc aagaatcgag
    27781 aaaaaggcga atggcaagta atgcaacgcg ctaactttca tcgttcccat aatgaatgtg
    27841 ccgctgactc cccacggtac gagagaattg acggtcgcac ctgaatccgc tagtgttcgt
    27901 gtcaaatagg tttttgataa gccaacgtca tcaaaagaac gcttgaatgt ttctcctgga
    27961 agaatgatgg acagatactg ttctccaacg atcagattga ttccgataca gctcaaggca
    28021 gtaaacccga ctaaacgaga aggtcggtcc atcttttctt ttaattcttg aacgatggta
    28081 tcaacgatca gatatttgat cagtaaccca cctaatgcca atgcaagtaa aatcaaacta
    28141 gcagaaccta acatactcat gatcccgcct cgagaaagca gttcatctag ctttggatca
    28201 ggtgtatttg cgacataacc attcatcaag atgtctgctg cttttccgac cgtcaaactt
    28261 ttctcatgga taaaagaaag aacgagagca gctgtcgatc ccgctagtaa tgtagggata
    28321 gccggaattt ttttgattgc ccccccaaat aaaataacta gtggaatcaa tgtccaagga
    28381 gagatccaaa agctcgcttt catgttttgg atcatttcat tgatactagc tagatcggct
    28441 gaatgactgt tatgtcctaa tatcgtataa aaaatcaacg aaaggacgaa tgctgggaaa
    28501 tccgtataac gcataccagc gatatgttcg aatagattaa ctcctccaat acccgctgcc
    28561 aagttggtcg tatctgataa aggcgagcag ttattcccta aaaaggcacc ggaaacgact
    28621 gcgcctgtag tcattgcatc attgaatcca agtaaatgac cgatccccaa aaaagcaatt
    28681 ccaatcgtcg aaatcgttgt aaatgaactg ccgacagtca ctccgacaat tgcacaaaca
    28741 agaaatgctg tcggtaaaaa gaaacgcaca gaaacaagtt taaatccata aaccataatc
    28801 gtagggatgg ttccagataa aatccatgaa gcaactaaga caccaatcaa aagaaaaatc
    28861 aggattggaa tgatccctgg agtgattcct ttgatgatcc cgtcatggat ctcatcccat
    28921 gtgaactttt taaatctgcc ataaaacaac aaacacatga aagctgcaag gatcgggata
    28981 tgtggcgaga gctggaatcc gatgatcagt actcctagga gtgccaacaa cacgatcaat
    29041 acagcgatac tttcttttaa agaaatattt ttattcatcg tcgatctcct atttcaaatg
    29101 aaaaggccct gccatgaaaa aactattcac ttggcaggac ctcctactaa atcagcgaca
    29161 aaacgtcggc tgaaagactt atttttttat ttttcttcta aacttttttc tacctcaatc
    29221 ccccaagaat ctttcttgaa gtgattgatg atcaatggaa taacgaacat gaccactgtg
    29281 atagctgcta ctaataatgc atcactcaat gtcgctgtac ctaatgtagc aaatacgaca
    29341 acgatgatag agaagatcag catgcagctg actacccaag ctaatccgtt tccgttttta
    29401 ccaattcggt aaggacgttc gaattctgga tgtttcttac gcattttaag caatgaaaca
    29461 gcaatcaaaa tgtacacgat acaataaata acagttgtag catttgtaag tgtcaagaat
    29521 acagcactta cgtcatccat tacaccataa agcaaagcaa ataatgaaat aacgattgat
    29581 tgtgttagaa cgacatttct agatacgccg tatttgtttt ctttatggaa tccgaatttt
    29641 ggtggaagga atccttcacg agctacttgg atgattgttt ttgatggtcc agtaacccaa
    29701 gctgataatt gtaaaagcac accaataaat accatgaagc tgaagatatt tccgatcact
    29761 tctggtaagc ctaggatttg acagtcaatc aagataggct gcgtgatatt tgctaattcc
    29821 atttttccat ctggtacgac atttgctaca agcatcgcat tgatgacatt caataaaact
    29881 aatccgatca aagcgataaa tacgcctttt gtataatttt ttgttgcgtc tttcaaacga
    29941 ggcatgtaaa cagaactcat ttcgatccca acaaaaataa atgtgatacc agctaagtat
    30001 ttcaatgaat caatatgttc caagtcaggg aaagcttttg accatgaaaa gtcacctaga
    30061 tagccgtttg agactagtcc gactttgaag gctgccaaaa cacctaaaac gaacatgatg
    30121 actactggga tatatacccc tagccataca ccgatgttcc cgccgacttt tgccatatcg
    30181 aatttcaagt tcaagatcgt gatgatccag tagataacta aaatacaacc taaaacgaac
    30241 cagtgattat tgccaagttc tacattacca aatgtgtttc ctaacaatgg tcctaaagta
    30301 gaagcgacca ttaccatacc tgggaacatt tgcacccata atagccatgc aacgacaaat
    30361 ccccatttgc tgcccagtgc tgtttttacc cataactgag gaccgccttc ttgaggaagc
    30421 atcgtggaaa gctcaccgga aaccattgat atcggaccgg caaaaaacaa aactgcaaaa
    30481 attgaataaa aaatcaatgt ccaaccgact gcggctaacg taggaatact ccgaaccgtt
    30541 gcacacagtg ccattgtcag accgataaag gtcccaagac ccattttact tacttcagat
    30601 ttactcaccg ttgacactct ccttgatttt ttggataaaa taacggtgtc tttgaagtag
    30661 tttcagcaat aaacgaaaca attcagcgaa tatttgaggg gattctttga gtttattgct
    30721 tgaagggttt cttcaaagac aacctcaaaa caaagggatc gaagagctta ggtctcccct
    30781 tgctcttcag atccaaacat gcgtcagaaa cagttagtat tcaagctgtt ttctaacatg
    30841 attaccttgc ttattttgct tcgcttgcca ataattggtc tgcgtagatt ttttccaatt
    30901 tttcttgaag agctgctttg attttttcag catattcttc aaagtgttct tctttgttca
    30961 tgtatggtgt cattacgctg gcacgcaata cagttacttt tccggcacgg ttccattctt
    31021 catctgagaa tcctagttgg ttcacaaatt gtaatgggct gttgccatag tctgggatag
    31081 caaagtcagt atgtgaagtt aagaactcat taccgtaaat gcttcctttc acgtatgaag
    31141 aataatcata tacgtcatgg ttcaatttat tcattgcaac taagtcatcg ttgccttttt
    31201 ctttgaatac atagtctacc atattgaaat ctggataagt taatggatga acttcgattt
    31261 ctttgtctcc tactttaaat gacaagtcat tcaagaagtt gtagaaacga tgtgcacctt
    31321 cgattgatgc acccattaat ttaccatatc ctgtgacatt caaaggtaag acatggtgtg
    31381 ctgcccaaac gctggctgct gttgcccctg cttttgaacc ttccaagata taagccccaa
    31441 gtaatgctgg gatatctgcg cctttttcaa atacatatgt cgcaaagtaa gagataacgt
    31501 ctctcatgcg gatatctttg atgacgatac caccggcaga atatggaaca tagcccattt
    31561 tatgtgggtc aatcgttaca gattctgctt cttcgattgc tttatatgca ctgtgtactt
    31621 cttctaagat ataatctttg ttttctgtaa agacattgta tttgtaatga acatctttta
    31681 aatcttcaaa tggaatgaag ttgttgtctt cgtctaagaa aatcgcacgg ccgtatccgc
    31741 cataagcagc atctacgtgt aagtagaagt aaataccgtc tttttctaat acgcgacgca
    31801 atgcaacgat tttatcgata ccgtcgattg ctccttcttc tgttgatcct acaacaccga
    31861 ctacacctag gattggtgtt ttttcagctg ctagaccacg aacgattttt tcaagttcgt
    31921 tgatgtccat gcgatagttg tggtcaactg gcactggaat cacttggtct aaaccgatac
    31981 cgatgatgtc ggcagctttt agccatgaat agtgtttagt ttgtggaacc aaccattttc
    32041 ctaatttttc taaatgtttt ccgctacgtg ctgagtgagc tttgatttcg tcgatttttt
    32101 ctgggacgct gtctaacaga ttcatgattt cttctgttga caagttcatt aattcccaat
    32161 cgctttttcc agcaactagt tcaggtgtca cttctttcat tgcaagcggc aatgatttga
    32221 tgttgcgggc ataccaaagt ccttctaagt ttgctaaaga accatcagca acgatgtggc
    32281 cccagccatc tttatagctc attaattttg caaattccat tcctacttct tcttccattt
    32341 ggcttgttgc aggagaagat tcataggcaa cgttattacc attccatagc atagcaaaat
    32401 tgtacgctag taaagatggc atcaatgttt cagagttcat atgaccccag taacgtccgg
    32461 cattatgcca aggaacggaa tgggtacgca tccgcgctga aatttctgat agaacatcct
    32521 ttgttttatt cacagtgtgt tcaaaactag cactgctttt ttcttctggc gtaataatag
    32581 gcatgtcttg aggcatgtag ttttgacgcc agcctaaatg ttcatccacc aattcattca
    32641 acaaagcttt atagatttgg ccgttctcag ctttgtcccc aatgaatagg gcatttaggt
    32701 ttagatcttt cgacaatgat tcactcatag tgaaaccctc ctaaaaaaat atttaaattt
    32761 tgtcgagccc tacattacac ctgttcttct attctgtaaa ctaagttttg gcctgatttt
    32821 taatcatatt ttaatatgta tcaattatcc gaattgttca cacttttgaa ataattaaaa
    32881 aaaacgaggg aatttacaaa tgtaaactcc ctcgcaaaga ttgtattaac gacaaccagc
    32941 gttctatttt aggatgggtt tcaaaattgg aatgttgtaa tagtatttcc ctttttcaag
    33001 gtaaaaactt actaaattct aatcaattac tttggccaaa aagtaatttt tcttaccttt
    33061 tcgaataacc acaaacttac catcgaattc atcggatgga tttatgacaa aatttaaatc
    33121 ggtaacgcgg tcaccgttaa tacttatagc tccgtttgaa acatcttcac gtgcttgacg
    33181 tttagatggt tcaatttttg tggaaacaag cagttccacg atattttcag gtgtgcttga
    33241 aatttcaacg ttcggcattt taccgaatcc ttgcgcgatt tcttccgcat ttagttcttt
    33301 aatatttcca gagaacaatg cttgcgttat tttttgcgct tctttcaagt cttcttcact
    33361 atgcacaaat cttgtaactt cttctgccaa acggcgttgt gcttcacgtt tctcaggttc
    33421 tgtctctact tttttcgcta agtcttcgat ttcttcttga gacaagaaag taaagaattt
    33481 caagtatttc actacatcgc gatcatcttg attcaaccag aattggtaga actcaaatgg
    33541 tgaagttttc ttcggatcaa gccaaacggc accgcctgca gtttttccga atttcgttcc
    33601 gtcagccttc agcatcaatg ggatcgttaa accgaacgct tctgcttctg gtccttctaa
    33661 ttttcggatt aggtctaatc cagctgtgat atttccccat tggtctgctc caccgatttg
    33721 caattgcaca tcgtatgttt tatgcagatg caaaaagtcg atagattgca ggatttggta
    33781 agtaaactct gtaaatgaga ttcctacttc caaacggctg gcaacgatat cttttgctaa
    33841 catcgtgttg atgttgaagt ttttaccgta gtctctcaaa aattcaagta aagagatttt
    33901 tgacaaccag tcgtagttgt tcacaaatgt gatatttgca tctttaccaa acaattttct
    33961 catttgattg gaaagtgcgt ccacgttatg ttgcacttgt tccatcgttt gcaacacacg
    34021 ttctgttttg cggccgcttg ggtcgccgat cgttcctgtt ccgccgccaa tcagaatata
    34081 tggatgatgg cctgccaatt ggaatctttt catcatcata aatggaatca aatgtccgat
    34141 atgcatcgaa tcaccagttg gatcaacgcc gcaataaagt gaaatgctct tttcttcaac
    34201 gagctcgcga agtctttctt cgtttgtttg ctgattaatg gcgtcgcgcc aagttaattc
    34261 atcaataata ttcattttac tgacctccgt gtagtttttt tatgaaagca ggaacctgct
    34321 agcatatgta attaattatt tcttcaggat aggataggaa ataaaaaaag tcccttggca
    34381 tataagccaa gggacgaaat ctcgcggtac cacccaattt gtatgtctag atatccaaca
    34441 tacctctcga acatttaacg ctgtcgcgtg gcaatcactg ccaaagactc ctgaaatgta
    34501 attcgcttta aagcatgtac tggtttgcag cgaccaccag ctttctgaaa cattggcttt
    34561 aacaactact tcgtttcgat cttcgtcttg ttattattaa tatgtgtcta tcatagagcg
    34621 caactttttc tttgtcaaca gaaaaaggat atttttttta aattcctcat tttttctttt
    34681 ttgaatccgc tctaattccg aacattttat tttcaattat tgttaaaaga aataactttt
    34741 tctgtatttt ttctgcaatt tatcgagaag tcactttact ttgcctctta ttctttcttc
    34801 gttacaataa ggaatgggac aagtaaaaaa atgaaaaagc aagggacgag gtatatggaa
    34861 aaaaagcaaa aaattatttt aggagccact tctgctttaa ctatcgttat tttaattcta
    34921 agtttgtttt ttgtcatccg atcatctaca aaaaaagaac caatcattcc tgaaaaaaaa
    34981 ataacagaaa aagcaaaaga accgacagat gataagcaac aactagttac tcaagcaaaa
    35041 aatctggctc aaagctacga ttatgaccaa gcgattgcgt tattggaaaa agatagtaag
    35101 aacggatcta ctgaaacaca acagcttctt caaacactaa aaaaggagaa agaacaactg
    35161 atcgactggt ctgatcctac tcagatctct catgtctttt tccacagctt gatcgtggac
    35221 ccgcaaaaag ccttccactc tcaacaggca caaggataca aagattatat ggtgactgtt
    35281 gaagaattca accgttcgat cgaacaactt tatcaaaatg attttgtttt agttaatttg
    35341 aatgatttga tccaaaaaga cgaacaagga aatctttcct ttaacgggct tgcgctacct
    35401 gaaggaaaaa aaccattggt tctttcacaa gatgatgtca gctattatga atatatggat
    35461 aattccggtt ttcctagccg attggtcgta tcaaataaca atgaaatcaa aaatgtctat
    35521 aaagagaaag ataaagaaga aatcggtgac tttgacatgg ttcctctgat cgatgccttt
    35581 gtcaagaaac atcctgattt ttcttatcga ggcgcaaaag gtaccttggc cctgacaggt
    35641 tacaacggag ttttaggtta tcgaacatca aaatctcaat atggagacaa tgaaaaaaca
    35701 aaaaaagaga ttaaagaagc taaaaaagtg gcggaacagt tgaagaaaga cggctggagc
    35761 tttgcttccc atacatgggg tcacttgaat atgactcaag cttcactaga agatgtaaaa
    35821 aaagacaatg aattgtggca gcaagaagtt gcacctatcc taggaaaaac aaatattctg
    35881 atttatccat ttggtgcaga catcagcgac tggcagcctt attcagcaga aaatcaaaaa
    35941 tttgcttact tgaagagtca aggtttcgat attttctgta atgtcgatgc ttctacacca
    36001 gcttggggac aattaggtac ggatttctat cgaaatgctc ggatcaatat cgatggcatt
    36061 cgttttcaat ctgatttaga cggaaaaaat cctattcttg atccattcat caatgtccat
    36121 gatgtctatg acaaagaagc acgcgaatct gcttgatttt acgtcattta tgaaccaaaa
    36181 aagccgaact gacattttat cgtcagtttc ggctttttta tgctttattt cttcttcggt
    36241 gcgattccct tgatcaggac ttctgtccat tctgctgtat atggaagcag ttttgcgtat
    36301 atctccggct tagaaattcc ttctaccatt gcttgaaaat agaggatcaa gaactcatcc
    36361 gaatatttca aatccacttt tccttctttc cgccctcgat ggaaaagatc aagcatgata
    36421 ctgaaacttt cttgcgtata cgcatccatc gcgtcagaaa attctttttt ttcttttttt
    36481 gtatagtatg tcatcaaatc gatataaaat tgtttgttca ctcgattgag ataacggatt
    36541 ttgttttcac tcatggcaat cagtgtttct tcaaatggaa gattttccgc aatcaacgct
    36601 ttcgcttctt gccctaaatc attcataaat gcaaaaaata cttcataaat cagcccttcc
    36661 ttgctgccaa aatatttaaa aatcgtcgct ttacttacct gagcgttctc agctacctta
    36721 tccattgtca ctccagcggt tcctatgtct gtgttcatca agtcaaaagc tgcttgtaaa
    36781 atctctttct ttttctcttg tgctctcttt tcaaaaccgt tcatttgtag agcctcccgt
    36841 aattttaaac ttttataata tatatagttc aaatttattc tgaggtttat tataactatc
    36901 tattgagaaa gtatcaagct agcaattcct tgtttatgaa ttcattgaca gaaatttata
    36961 aaaagaatat tatgtactaa aataataaaa aaagttttat aaaaaggtat aaaacctttt
    37021 tattaaacta tttaatcttg tttagtatat atttttagaa aaaggagtgg ggtttttatg
    37081 gcagaaatcg tcaaagtcac acatttgaaa aagagctttg gtcgtttcga agctttaaaa
    37141 gatgtctctt tcaccgtaca ttcaggtgaa gtcgttggat ttatcggacc aaatggttct
    37201 ggaaaatcca cgacgattcg gacactttta gggattataa aaaaagacgg cggaacggca
    37261 acgatttttg ggcaagatgt atgggaagat agtttatcta tccatgaacg tctttcttat
    37321 gttccaggcg atatctcttt gtggggaaat ttgactggag gagcgatcat cgatctcttc
    37381 atgaagcttc atggaaaagg gagtgctcaa aaaaaagatg aactgattca acgtttctct
    37441 cttgatccta agaaaaaagc aaaaaactat tcaaaaggaa atcgccaaaa agtgggcttg
    37501 attgctgctt tggctgttga ttcggatctc tatttatttg atgagcctac ttctggttta
    37561 gatcctttga tggaaacgat tttccaagaa gaagttgaaa aattgaaaga acagggcaaa
    37621 tccatcctac tttcttctca tattctcagt gaagtcgagc gactagcgga taaagtcgtg
    37681 atcatacgcg aaggaacact cgatgaagaa ggaacactcg atgaattgcg tcatttgact
    37741 cgttcgacca tcactataga aacagaagca gagaatagtc acttagcaca attgaacggt
    37801 gtccatgact ttgtgcaaaa aggtcacctc gctcaatttg ctgctgataa cgaagctttc
    37861 gatgtgattt tagctgaagc gtccaaatta ggtgtgaaaa aatttgaatc tattccaccg
    37921 actcttgagg atttattcat gagacattat gaaggatgag gaggaagcga aatgactgat
    37981 aaattttcac gctggtccac ccttttttct caaaacctta gccgtgactg gaaaaagatc
    38041 ctgatatggc tagtcggtgt cactgctttt tctggtggtt ttgtccctgc gtttgaagaa
    38101 ttgacttccg gacaaggatt agctggatta tacgaaacct tgcaaaaccc ggcaatgatt
    38161 gcaatggtcg gaccaactcc agcttcgaat gctgctgagt atactttagg agcgatgtat
    38221 gcaaatgaaa tgttgctttt ttgcagtttg tttgctgctg tgatctctgt tttacacgtg
    38281 gtgagccaaa cgagaaaaaa agaagaactt ggtctgattg aacttattcg ttcctttcga
    38341 gtcggacgtc aagcaaactc tttagctgtc atcgctgaaa gtattctgat caatttgctt
    38401 ttagcaatat cgatcagctg tacgatgatt tttttccaag cggattcgat cactgcagaa
    38461 ggtgcttggc tttttgggct atctgtaggt agtatgggga ttttggggag tgccgttgca
    38521 ctgtttgctg cacaactgat gccaagttca gccagttcga atggtctgtc tttagcgttt
    38581 ttaggtggac tttaccttct acgagcaata acagatgtag ctgattcctc cctttcgatg
    38641 tggaatcctt taggctggtc ttatttgacc tatccattta caactaacaa ctggattcct
    38701 ttgctttatt tgtttttgtt cagtctaatc ttgataatca gttcatttgt tttagaggaa
    38761 catcgagatt tgaacaccgg ctatatcccc gaacgtgaag gaaaaccgta tgctaaacgt
    38821 tctcttttat ccttgcgtgg tttattattc tatctaaata aagggatgac aatcagttgg
    38881 cttatcactt ttggtttgat gggggcggca tacggctcta tctacgggaa tatgcaacag
    38941 tttttagaaa gtaacgactt aatgaaacag atgtttatcc aatctggcgt ctcaattgaa
    39001 aaaaacttta caagcacgat catggtagtc ttgatttgtc tagctgctat tttaccaatt
    39061 gctagcatca atcgtttatt tacagaagaa acccacggac gtatgaatca gctgttcgca
    39121 acaaaaattt ctaggaacca actttattgg accaatatgt tactagctat tctatttgct
    39181 atattggcaa tcctgattag ttctggtgca ctagggcttt ctgctttggc ttcgatgaag
    39241 gatgcgaaag acatggatct attgatgttt cttaaagccg ggttcaattt cctgcctgcc
    39301 gttttagtgt ttatcggaat tagtggcttt ctattcggat ggcttcctcg aatcggtaaa
    39361 cttgcttata tctacttagc ttactccttt gcactcaatt actttggcgg aattttggat
    39421 ttaccagaat ggttttccaa aacagctgtc cctagctggg ttccacggat gccaaacgat
    39481 tcttttgatc tttccgtgtt ccttacgtta tcatgtatca gccttgcact gttcgtcctt
    39541 ggttctattg gttataggcg aagagactta ctagaaggat aataaaaaag catgtacaat
    39601 ttcatgctag cctccaaatc tgggtcaaaa atccaacatt tggaggtcat ttttttattt
    39661 acaagtcttc taactgctct ttatcaatga tccagacttc tcgattcttt ctttttacat
    39721 aaccattctc ctctaataat ttaaattttc tagataaagt ttcgggggtc gtaccgataa
    39781 atgcagcaag ttctttcatt ttcattggca ggaaaaagtg atccttccct gtcgctttag
    39841 ctaagtcaag cagatagaca gcgactcgtt cttctacttt ttccatcatc aaaaaagttg
    39901 tctgctgctg tacttttgtt aatttctcaa cgttgattgc taaaagtttc agactaaggg
    39961 cgggataatg cagtaagagt tccgagaaat ctttttgtct caatatgcaa atttccgttt
    40021 tttctagtgc ttcgccaaat aaagactcat ttggaacgcc tagcaactgg ctctctccct
    40081 catatccgcc cggctctatt actcgtaaaa gctgttcctt tccgctagca gataattgat
    40141 acactttcat actgccggag gccacgatca ctaattgagg ttctccattc ggcgcaagaa
    40201 tctgttctcc tttttccaca tagtgatgtt tgacaagtgc gtgtactttt tcttgatctt
    40261 gctgtactaa gtgattgaac aaaggaacaa atgaaacaca taaatgatca tgactcataa
    40321 tctctcctcc ttctaaacag ctatttctaa taaagaaaca taacttccct tattccaact
    40381 gataattttc ttttatcata gcataaacgt caagcgaacc agataccagc aaatagagtg
    40441 gatttctttt tgtcccttgc tttattttcc ttcttttgag accttaagta attgattcct
    40501 atcaattttt taaatattta ctttttgaat aatcaaaaac aaaccttcac tcgcaagccg
    40561 agagtaaggt ttgtttttcg cattgcttat ttcgtgttgt ctattgaaat ttttccgcac
    40621 ctagcttttg atatagtggt gctagttctt gtaaggtttc agcggttatt tgctcaacgt
    40681 gttcgatttc ttgcggatag aagaatagtt tacccatttg aaaggttcgg tctctcatcg
    40741 cttttttcaa ctcttcttgt ttctttgtat cggacaaata gtcagacaaa gctaagtggt
    40801 cgtcaagttc atgttctggt tgcggccata tgtaataatc cttgatatcg actgttttgc
    40861 tccactcttt cagtatcgca agcaactggt tatacgctgc aatgctacca tatcgttctt
    40921 cgcttttata atgttggtac atcaaataga tttggtattg tttttgattc aacaaaactg
    40981 ctagacaggc attctcgtct tgacgagatt cgcttcggta cgcgctccaa aaatgactgc
    41041 gtaaattcca gccattcgtc cagctttcga ttttaggttt gctcatgccg tacatatcag
    41101 gcaaaagtgc agctgtctgg agctgaatct ctttccattt ttgccagtgt tctttatatt
    41161 ctttcttgat ttgttcaatc ttatctgttg gatggctctc tttgagctgt ttgaatgcgt
    41221 agattggtcg atcaaacaac tgataatctg attttccaag ttccattgtt tcgcctcttt
    41281 ccgttatctt ttgtattcta cctgaatttt gcgatatgga caaatagagg ttgtgaaaaa
    41341 gctgttctgc atcaagtcat aagaacagcc aaacaaaaaa ctgcgatgag catcaccgca
    41401 gttttattga acttaaggtt gattgatcac atctaaaagc gttgaaaata aaatggcact
    41461 agaagcagct cctgggtcga tatgtccgat tgcacgctct cctagatagg aagctcgtcc
    41521 ttttttagct accaagactt tggtgtgttc ttttgcctgg tcaatgattt cttttgttaa
    41581 attccctgcc ttcaatgcct ctgtgactgg cagccaaaca tctaacatcg ttttatcgtc
    41641 agcttctgcc tttcctcggc tttgaatacc cgctgttccc tgctcgatca cttcgcctaa
    41701 tttttcttga gaatcaatag aatcgacctc ttttgtcgct ttcatcatgt tcatgaatgc
    41761 agatccgtat aacggaccag aagccccacc gactttcgat atcatcgcca tagagaatac
    41821 tttgaatgta tcgctgattg tttcaggatg atcggcttga aaagcttctt cataagctga
    41881 aacccctctt cccatgttat ttccatggtc tccatcgcca atcggggtat ctagatcact
    41941 gagatattgt ttgttctgat tgattgcctc tgcaaattgc gtgagccatt cttggatttc
    42001 ttgaacggtt agtttcatat tatcaccctt ttatcttgag attttttacc atgaaatcgt
    42061 ctgtacattg ctttctaaag aatctttcca ttgtgtctcc gtcaagtcga tcagtgttag
    42121 agaaagacct tgcatatcta gtgaggtcat aaaattaccg acttttcgga aaactacatt
    42181 gacgcctctt tcttccaaca gagctaaaac atcattcatg aacacgaact gttccatcaa
    42241 aggcgtcccg cccattccgt tgactagtac acctgcttct tttggtaaat tcgagtaatc
    42301 atctaaaatc ttagtaacaa gttcctttgc cagtacttta gaaggctgca tcttttctcg
    42361 acgatagcct ggctcaccgt ggataccaac accatactcg atctcatctt ctcctagttc
    42421 gaatcctggt ttccctactt ctggtacagt tgctgctcga agcgctagac cgatcgtctt
    42481 agttgcctgt acgacctttt ctcccagttc tttcaactca gctagggaag caccgtttct
    42541 tgctgcatcg cccacgattt tgtgtacaag gacagtacct gcaactccgc gttttccagc
    42601 agtatacgta ctattttcaa cggcgatatc atcatctacg atcacacttt ctacttcaat
    42661 atcgtccata gcagctaatt cctttgccat atcgaagttc aatgcatccc ctgtataatt
    42721 cttaacgatc agcaaaactc cttttccact atcggcttcc ttaatagccg tctgaatctg
    42781 atctggagtt ggagaagtaa acacatcacc aagcaccgct gcacttaaca tcccttcccc
    42841 tacaaacccg gcatgggatg gttcatgtcc gcttcctcca ccagaaacca aaccaacttg
    42901 ttccgctttc tgattcttgg ctacaacacg ggaagattct acacgatgaa caagttctgg
    42961 atagcttttt actaatcctt ccagcatttc ttcgacaata ttgcttggat cattcatgat
    43021 ttttttcaat ttattcccat cctttctttc cggcattttg tgcttctgtt aagatttgct
    43081 gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa agctccttcc actaaaggcg
    43141 catccaccag atgataacgt tgctgctgct cttcttccaa catgtctttt gccagttccg
    43201 agttcaatac cgcgctaccg atatccgcaa aaacaaaata actagctgca tcgcttgatt
    43261 cattgactgc ttctacgatt ttcatcgggt ctgatccgat ctcgccattt attccgccga
    43321 gagaaaaaat ttgcacctct tcggattttt gcatctgctc aatcatttct ttaataccat
    43381 ctgttacttt ttgactatga gaaataagca aaatactttt tttcacaaga atcttccttt
    43441 cttttacata gttgctaacg attggtttcg ctttcattaa tgaacaattc aagtatataa
    43501 taaatttcta attttgggaa gatatgtgta gtgcttcttg aagtttgtta tactttaatt
    43561 aaaagagagg agtgatcgaa tgattacaac aacaacgaac agtgtagagg gaaaagccgt
    43621 agtagcttat aaagggatcg tttttggtga ggtcattact gggatccatg catttaaaga
    43681 cttggaggcg gggttgcgaa atatctttgg cggccgctca aaaagctatg aaaacgaact
    43741 aatgggtgca agagaagaag cacttgccga aatggccgaa cgtgcaaaag cattaggagc
    43801 tgatgcgatc attggcgtaa aaatggacta tgaagtatta ggcagcgaca atggcatgct
    43861 gatggtcaca tgtagtggaa cagctgtctc cacgagataa gtaggggaca tctgttttaa
    43921 taactaaaga gagatgggac atgtagtggc actcaagaat tagatttttc agtctaactt
    43981 cgaggtactc taccttccca tcctctcttt tttatttctt tttgcggatg attccgtaag
    44041 ctgctgcgac taacacaccc gcccctgcta aggaataact gacagaagct gttttttcat
    44101 ttgtttttgg caaagtctga cttgttgatt tggatggctc tgtcgtttgc tgaactgttt
    44161 ctgtcgtgct tccagaggtc actgattttt ctcctgccga atgcaaaata tcttttattc
    44221 gttggaaact agctgctaag tcagcttttt ttgcttcaaa atcattagca actgtttgtg
    44281 cttcgctgat ctttccgtgg attgcttgac tggccgctgt gatatccgct tgagtttgac
    44341 ttagctttcc agaagctgtt tgagcaacct gatttaattg ctgattgaat tcttttgctt
    44401 gttggatctt cccgtcaaga tctgtttgga cagcttccgc ttttgctttc gcttcggcaa
    44461 gctttttctc aagttgtgca cggtcatttc ccactttatt tttaagatca gctaaatctt
    44521 gatcgattgc tgcttggtct tctttcaatt tgttttttgc agcttctaat gcttcttcga
    44581 gttcttttct tttattttcc aagtcttgca aatgatcgat ttggtcagaa gaggtagaac
    44641 tatttattga tgtatcttct gaagatgaaa tcgtttctcc tgaattatct gtcggtacat
    44701 tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc agaagagctt atggttgttg
    44761 aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc tgtctgtccc aataagaaaa
    44821 tactggaaac agcaattggc cagattaacg gcttcattct ttttttcatc acttttccca
    44881 tccttactac tttttaacta atttcttatt ataagcaaaa atgtttggaa gaaccataaa
    44941 cacaacaggc tgtttgtcta aaaaacggtg catcttttat ttttttgtac gttccgggct
    45001 tttccatgtc ccgcttccat aacctaaaat cgttttgact gctggaatga aagtcgtcac
    45061 gatcgttatc gcgttcacta tccagaacaa aagcatatat agtggtgcga aaagaaggta
    45121 tttcaatttt cttcgtcgat catctacaat cagcgaagct agcaactgga aaaatcctgc
    45181 gaccatttca aagcagatga acacaaaact gaatgtaaaa gtaatataaa tctgttcgta
    45241 gtttccttga tatacataat gcccgattaa tccagcaaaa agaactacgg aaatacaaaa
    45301 gaatattgac cagacaatac tcaatgtttg atcaatgaag atgatcgttc ttccaatatg
    45361 tttaaaagga tggagcatca cttttttgaa attcgtcagc cagacttctg ttccaccttt
    45421 tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc acaggtactt ccatgaaaaa
    45481 gataatctca ggagcaaaaa cagataacca gtcatttaac tgatgatccc aagcaatact
    45541 gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca tcgatcaagg cttcttttcg
    45601 atacatcgta tttgctccac tgtacgcata aagaccaccg aaaacagctg actgtgttcg
    45661 tttaatgatg cccacgatac ttgagaattc aaccgtttga gattttgcaa tcaatttggt
    45721 acgattttgg acatccatat ttgcagtgac cgcagcaata tgtcttgcac cagggcgaat
    45781 gaaataattc acatacctaa tcagtgcatc tggctccggt accgtgtctg catcattgct
    45841 taaaatcaat ttcccttttg caaaggcaag tccgatatta aacgcgtggg cttttccttt
    45901 atttttttcg atccgaacca cacgaagatt agcgtatttt ttcatcagtc tagctaatat
    45961 ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat acctcatagt tgtgataatt
    46021 caatttcgtc atcaaatact cgattgtata ttcgatcaca atttcttcat tatgggctgg
    46081 caccatgatc gtgatgaacg gttcgacact caagggaaca tctacccatt ctttttgctt
    46141 atgtttgtat aaaaacacat aacaccagac accaataaac catacaaatc cgcctaaaat
    46201 cggataagaa acgagaaaaa aatacataat agacaaacaa gtatctaaga ataaattacc
    46261 tgtatgggtg attgagatca tttcagttcc accccatttt ctttatacaa atctgcaatg
    46321 aatgtcgtat ctagattttt ctcttcggag acagaataaa aacggaccgt ttctctctct
    46381 tcttttgttc caaaacgttc cgtatacact tcttcaagta attcttgtct ttttttcaat
    46441 ttttcatctt catattcaaa agatttattt acttttttgt atagatttcg attgttccac
    46501 ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt
    46561 aagaaataca gcattttctt ttctgataaa aaagcgtaca aggaaaaatc cttggctctt
    46621 tctggtctcg ttaatgggaa aaagacccat ataaaaggaa gaacaacgat catccatcct
    46681 aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat taccttttga aaaatactta
    46741 tctttcacca aaattgatgc aacttcttct cttgttactt ttttcatatc ctcacctcac
    46801 gtttttttgt tacgacgtag gagtttacca tacatttcat ttcttttcac ataaagtgtt
    46861 cctaaatttt tcctaagaaa aagaacaaaa catttctcat cagcaaatga aaaatatttt
    46921 gttcttttta tcagttgttc tttttatttt ttatctctta attatgttgc tctttccaat
    46981 acgacaatgc ttttcattcc tctagatatc gccgtgtaca tttgtctgcg tcttctttgc
    47041 tcatctccat cgttttcaaa tggatataag attacatgat caaattccag tccttttgct
    47101 agactcaaag gaatgatttg gcagttttct aatccatatg aatttttttt aatcgattct
    47161 tgtagcgttt gggtttcctc ttctgtttgg gtgataactg ccgctttttc tccttttaga
    47221 gaaaccaaaa tctcagctag tattttcaac aattcttcag aggatcgaaa agagcggtat
    47281 tcgggttctt ttccttgcgg tcggatcgat acgatgtcga tcttctcttg atccacagca
    47341 taggttttga aaagctctgt gattgctccg ctagaacggt aactatttct aagatcatac
    47401 cgatggatcg gcagatggta gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa
    47461 gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa agtgagtttt tggaaataat
    47521 tcgatcaata gacctaattg tgccagggta taatcttgta cttcatcaat caatagatag
    47581 cgaaatgctg gtacatttac cttttcaaca agtaaatgac gaatagctaa tataatgact
    47641 gcttcatcaa cagtacgtgt gggttgccaa gcataagctg agccatatcg tttttcataa
    47701 attctttcaa acaactgatg ctcatccacc catgccattt cttcgatttg tctggttatt
    47761 tttcgatatt ttttttgtaa aagtttcctt gcataagcta caatactttg ttccgaatca
    47821 tcagaaatca atttaccgaa taattccaac tgcctatctt ctgtcaacga gagtacctga
    47881 ttctgcacag caggactttt tgcttgtttc aaaagatgat tcttccattc gctcatcagt
    47941 gcctttttcg ttgcttgaag cctatcgatc aatttaggat gagcaggaac tttctcatat
    48001 attttttcga tcgttttttt agaaaggact gtttttccat ttttagtaag tccacggata
    48061 aaattcggat gatctaaaaa caaaggatct gattgtttta gattatcgat aaactttttg
    48121 ctacgcaaac gttctgtctg ctccgataca ttttctcccg tgatacgttt gaaataagcc
    48181 tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca gctgtatgat ggttagattc
    48241 agtggatttc tttcacctaa tgatggtagg acattcgaaa tatagtcgat aaaccgatga
    48301 ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat ctcgatataa atagagcaga
    48361 tacgcaatac gctgcatgat agcagaggtt ttaccacttc ctgctacgcc atttaccaag
    48421 atattttgac ttttcacatc ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg
    48481 atagccttca tttcattggt ctcatcttgg cctaagactt ctaataatac gtcatcttga
    48541 atagctacag acgtatcaaa aaacttgacc aaacgatctc tttccaaaag aaattgtcgt
    48601 ctttctttaa taaccgtatc gattttattt tgacgaaccg tataagaaga tggaccaatc
    48661 atgttattgt aaaaaagttc ggcaattggt gaacgccaat cataaacaag attttcatct
    48721 gcatcatctg tgaaatttgc tgtaccgata taaaaggatt cttttggctc attatccaga
    48781 aagtctacaa cgacttttcc aaagtaggga cttttcaata aacgttcgat ctttgctagc
    48841 tgattttctg cgtttttctg tttgatattc atctgatcga tttctctatt tttcatttca
    48901 atcatcgaaa aagtatccag gttatctaag taactgtcaa aattcaatcg aagctcgcca
    48961 gccatttctt tcaacgactg gtttccttct atttttgccg tatccatcga ttcccgcagt
    49021 tcgttttttt tatcaattag cttttgatac acgttcttca gatatgcttc ttctaactgt
    49081 ttttgtgtgt tgaccataca cattctcctc tttttttaaa gtggtggaac cgattagttt
    49141 acctctttta agagtcgaat gcaagctttt taacgagtat catttgttag gatatatcag
    49201 ttcttcaggt cggacagttt gatataaaga aaatgcacca tcaaggtttt ttacgtggta
    49261 gccagactgc ttcaagattc tttccgctag atagctacgt aatccgctat gacagctgac
    49321 gatatattct tgttggctgt ctaattcatt taatcggtcc cgaagttcat tcaaagggat
    49381 atgtttggct tccttgaatt tgcccttttc caattcttct gctgttcgca catctaataa
    49441 aatttttcct tttgctagtt ctgtcggcaa ttcatgccat tgtacggttt cactaagccc
    49501 ttctgcaatg ttcattgctg catatcctag catgtttact ggatctttgg ctgagccaaa
    49561 tggcggtgca tacgtcaatt ctaattccgg taaatcgaag atcgtcaaat gccctttgat
    49621 tgctgttgct aaaatatcga tccgtttatc cacacctttt gccccaacac cttgtgcacc
    49681 ataaatctcg ccagtctctg gatggaagat caacttcagt aaaatatctg ttgcttctgg
    49741 ataatagctt gcatgatctt ttcctgatac atgaatgact tcaaaagaca gttcgttttc
    49801 tttggctgtt ctttcactta aaccggtgga tgcggcagtc attccaaacg ttcgtataat
    49861 tgctgttcca ataccgcctt gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg
    49921 tctaccttga cgattggctg gtgaagcaag agaaataagt gcatcttttc cagtgatttg
    49981 ctgttttaca acgatagcat cccccaccgc ataaatatca ggaatattcg tttgataacg
    50041 ttcgtctact aggatacccc cgcgtatgcc taattcgatt ccagcggtca ctgctagctg
    50101 gttatcagga cgaacaccaa tggataaaat aactaaatca gcagggatgg tacgcccatc
    50161 atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg acagcatgag aagtaatcac
    50221 ttctactcct tgatgagaca attctttttc tataaaagct gccatttctt catccagtgt
    50281 tggaagaata tgtggtgctt gttcgatcac catgacttct aaacctcttc tttttaggtt
    50341 ttctgccatt tccaatccga tgaatcctgc accaacgatc acggcacgct ttggctgttt
    50401 ttctaatgca tgcatcactt tatcgatatc tggtacattt ctaatagaaa aaacattgtc
    50461 ggcttctgtt atccctggaa tcgatggaac aactggtttt gcaccaggag ataaaaccaa
    50521 tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg acttctaccg ttttgttttc
    50581 gggaaagatg gctgtcactt catgatttgg acgcacatct aaacaaaaac gagcggataa
    50641 tgattctggc gtttggacaa gaagattttc ccgttcactg atttctcccg aaagataata
    50701 aggcaagccg caatttgcaa aagaaacata cggtcctttt tcaaaaacaa cgatttctgc
    50761 atcttccatt aatcggcgaa gccgtgtagc agcagacatt ccacctgcta cacctccgac
    50821 aataacgatt ttcatcttat ttccctccta ctgttcgatc aaaccattga ttcatgccac
    50881 cacgtacatt cactacatca taaccagatt tctttaattc tttagctgct tgtttactcc
    50941 tcatccctga ttgacaaatc acatagacgg tcttttcttt gtctccatca tatctgttta
    51001 tactttgtaa tggaacattt ttagctcctt ttatatgccc gccccggtat tctgcaggtg
    51061 ttctaacatc aagtaacgct ggattttcca aaagcttttc ctttaattct gggactgtta
    51121 tcgattttat tgattgaaat agcaaattca ttttctgcac ctcatttaca tttttataaa
    51181 tacctcatgg ggtatattaa acaggagctt tattaatatg tcaaccagaa tgactcttca
    51241 tttttttcac tactttttat atttgcataa aataccgata aggggtatta tctaattata
    51301 aaaatagagg agaatgtgtt atggagattg ataataaaaa tatcctgaac cgtttgaaac
    51361 gtacagaggg acaaattcgt ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg
    51421 atgtcatcac tcaattaagc gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg
    51481 ttgcagataa tctgaaaaat tgctttgaga aacctgatag taatccagaa gaacaagcgg
    51541 ctaaactaga acaagcgatc aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa
    51601 gtcgttttcc atattgatga attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac
    51661 ctgataaaag cttctccaga aacgacgatt gtcgtatctg taaatggcat cgcgattacc
    51721 ggttatctag actctgccaa tgcagaattc ttagatttgc aaggagtcgt ttttcacgct
    51781 tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt ctttacctga acaagtaatc
    51841 gttgtaccag caggtgtcct tgatttagtt gaactgcaat cccaaggtta tgcttatatc
    51901 aagccttagt cagtagaggt tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt
    51961 tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt tgtgaaaacg ctgtcctgca
    52021 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcgttt ggttgggctt
    52081 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa atcagcggtt
    52141 tgacctaagt attagagaaa aaaacgggaa aatagctcct catattttgc cgttttttga
    52201 gctaataccg aaggtcagct gattgaacac cgtttaagga agagattgtg aaaaagtcgc
    52261 ttagtttcga gtattaagaa aaattttgtg aaaatggatt tctacatttt tgcataaatt
    52321 tggcttaata ccgagaaact ggcttttgaa taccgtttaa ggaagaggtt gtaaaatcag
    52381 cggtttgacc tgagtattag agaaacaaac ggaaaaatag ctgctcatat tttgccgttt
    52441 tttgagctaa taccgaaggt cagctgattg aacaccgttt accaagaggt tgtgagccta
    52501 ccatttaact ccgaatcaca agaaataatt tcaaaaacaa cttctcctgt ttttggttcg
    52561 gttggcttat tatcgagaag tagctactgg gagtctattt atcaaaaaaa ggaatgcgac
    52621 ataggtcaca cccctttttt attttctttg aaacatcttt cggtactcga cagcatacaa
    52681 gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa atgactctca cccacagcgt
    52741 tggtaattct gggaaaagca gcaaaaggat catgccaaca tagatgacag cagtcgctac
    52801 tttgccatac cacttcgctc cacttaattt gatattttta gatagcccga ttaatccagc
    52861 tacaaacata aagccttctt ttatcaagaa tagaccaaac aacagccaaa gccacggtcg
    52921 ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat ttatctgcaa acggatcaag
    52981 caccttccct aagtctgtta cctgattata tctccgagca atgaccccgt ctagataatc
    53041 agacaaacct gagacaacaa tgaccgctcc tgctatataa aatgactgtc tcagcacaaa
    53101 ataaagataa atcggcagca aaaaaatacg gaatatcgat aaaagattcg gaatcgtttg
    53161 tatttcttta cgccagtctg tgcttaattt cattttatcg ccctacctcc tcagcactct
    53221 tttcttttta ttttagctta cacggggaat aaaaccaatg atttcactgt ttttattttt
    53281 ctttacagac actgatatta tgagctaatt tgagataatc acttgctgtt ttcatcgttt
    53341 actcttcttt cttcctgtcg tttgcgcatt tcttttacta gtactttatg agcttttttt
    53401 gatcctcgct ctaggtcacg ctgccgttta cgtgttttat aatcgataaa cagactgtct
    53461 aaatcgtaac ctgtcgggta cagatcttct gctgaacgca ccagacgaac acgctgacga
    53521 ggcacgtgga ttattttttt gtctaaatat acatcaatcg tatcttcacc tatatcttca
    53581 taaactaacg cttctttttg gtattcattg acaaatatcc gatcaccttt tgcaaagagc
    53641 tgacgttctt ttttattctc cttcctattt gtaactgctg tcttaggaag aaattctttt
    53701 ttctgtgtcc gataggcttt ttccgccaag taacgctctg cttgatggat caatttcatc
    53761 gacatatcca ttttatgagc aatccacaaa gcttggcttt ctcctgtttc ttccaacccg
    53821 caactgatat ttaggacgaa gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc
    53881 ttcatgatct ccggcgaatt tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc
    53941 cccttgctca tacatagatt ccatgattgc gatcgctagt gcagcccctt catttggctc
    54001 tgttccactg ccaatctcat ctaaaagtac caacgtattc ctttttatct ttttcaaaat
    54061 agcagcgatg ttttgcatat gaccagaaaa tgtactcaac gcattttcta aattttggtg
    54121 gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc tctgtccctt cttttgccgg
    54181 aacttgcata ccgaacatcg ccatcaaagt caataaaccg actgtcttca acacgactgt
    54241 tttgcctccg gcatttgctc cggtgataat caagcctcga tagtctttac cgatttcaac
    54301 atccaatggt accgcatgct caggtaaaaa tggatgtctg ccttgcttga taatgatatg
    54361 ttccgattta ttgattctag gtgtgcttcc attgatttct cggctaaatt ttgcccgagc
    54421 aaagatgata tctaacaccg tgatcgtttc gatcaataaa tcgatcactt cttcgttttc
    54481 tgcgatagca ccagtcaatg ccgccaggac ttgatattct tcactgattt cttcagcttt
    54541 taacaactga tagtgttcat tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt
    54601 tcttttattt gattcatcga taatcgttcc atctatttta tttttatagc tggctttgat
    54661 aggaatcgta tagtattcgc ctttttgaac aatcattgct tcttggatca tttctttgtt
    54721 tttcggatga cgcaagaatt tcagtaattt tgactggatt tctttttcga ttagttgtaa
    54781 ttgtttgcgt actttacgca aattacgtga cgcgtcgtca ctgactttat tattttttat
    54841 tttctggtca atcaactctt ctacatttat caaatcagga agatgtttgc tataagcaaa
    54901 caacaagggt gtctggtatt gattcttatc aaaaaacttt gtgatcatcc gactgcttct
    54961 aagaaaatcc gcatattcga tcaaatcggt tggctgcaga atcaaccctt ttttgacttg
    55021 ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga acatgctgac tgctttctaa
    55081 gatcaagcgt gcttctcttg tttcttcttg acgatccaag acagtttgta ggtttgtcga
    55141 gaccgtcata tcgctgattc gttttttgct atagtttcca attgctcttg cttggatttc
    55201 ctttttgatc cgatcaaatt gtaattgact aatcatttcg ttgtccatat tctctccttt
    55261 tcttgaagag atacctgtcc caaaataaat aagggcctag agataaataa aaggatagaa
    55321 cacagcttaa aaaagtcatg tcctatcctt attagaatac aaagatattg ttttcctgac
    55381 aggtaccgtt tcataagcac accaaataaa ctttgtttat tggctattcg acttactgat
    55441 tagataccag ctacactcac aaaacatacc ccattcttct aggtcttatt tattttccac
    55501 ttaactatag caagacacat tttggttgtc aaactttagt atgttttata gatttacacc
    55561 attaatgaat agacacctac cgcgataatt gctccgatca ttggacctgc gattggtacc
    55621 catgaatatg cccaatctga agttccttta ttagctattg gcaataccgc atgtgctaat
    55681 cgaggaccaa agtcccgagc agggttgatt gcatatcctg ttggccctcc taatgaaaga
    55741 ccgatggcta agatcaatcc acctacagcg aatacgttcg ttccaggtgc aaattcgttt
    55801 tgtccaaaag ctagcaaccc aagaactaat acaaatgtac caattaattc tgtgatcaca
    55861 tttgctggat aattccgtat agcaggacct gttgcaaacg ttcctaaaat cgcgctttca
    55921 tctttcgttg cattccaatg tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa
    55981 gctcctaaaa cttgcgcaat gatgaaagga acgacaagat tccaactaat tgcccctgtc
    56041 atcgccatag ctagagagac tgcaggattc aaatgagctg ggcccatgaa tccagaaata
    56101 tagacagcca ttgttacagc gagtccccaa ccaaatgcga tgacaatcca gccagaagcc
    56161 tgtgctttac ttttgtttaa attcacagcc gcacacacac catctccaag caaaactagg
    56221 atcagtgtcc caataaactc ccccatgatt tgagtcatat cactgttcat ttatttctcc
    56281 ccctttagtt cccgcagatc tgattctgca atcaatgctt ctaaacgttt agtctgttgc
    56341 gccttctcct cttctgtcca ttcaaaatat ccagcaattg catcgatcac aggttgcttg
    56401 atctcatcaa gctgatctcg ttcaaacaac agatgatttg tacgacgaat gagataatcg
    56461 cctggggcca aaaccatttc ttcttctaaa ccataacgta accgagctga ctcagcgaga
    56521 ctcaaaccag gataaggtgc catttctttt gctaattcaa agatacgtcg ggcattagtc
    56581 ccgtaaaaat cagcgatata agtagcatct tcttcctcta aaccggctgc tactccttct
    56641 tttgctaatt ctgtcactgt ttcttctaat ttagttggat cgaagtttcc acctgaaatc
    56701 tgatattttt tagagtcgat ttctttcgtc tctattccgt attcttcttt taataattgg
    56761 cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat cggtgatttt cccaccagat
    56821 aaagtcagca aaccatctgg ttctctttct aaagaactac ctctggaaat cgtagaaggt
    56881 gctttttcat cacggctgtt ttccaaatgg ttcaacacgt cttctacttc agcacgagaa
    56941 actttgtttt ccttatattc acttactgta tcgacaactt tattgaagct cttgtctgaa
    57001 atcgatccat tatctccacc attgtaatca gaaccagaat taccaatcaa cagtggacga
    57061 agtcctgccc agcttgcttc gatatcattc aatgtgatat ttgcttctgg atagcgatga
    57121 ttgatcacat ccaatagata atctacatct tcttgtgtga ctttcggatc cgtaaaatct
    57181 ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa
    57241 accatccgcc catcttgctt tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt
    57301 ttcgcatcta caactaaatg gatcccttta gttggacgca ttttaggaga gactggacgc
    57361 gtaaaattca agtttcgtac tttatctacc caaggaccac tcgtattgat cactaatttt
    57421 gctttgattt cgatcacttc atctgtcagc aggtcacgag ctttgacgcc aacgatttga
    57481 tcgccatcat ataaaaagcc aaccgctttc attttactta ctagataagc cccatcttcg
    57541 gcagcctttt tgatattatc aatcactaaa cgggcatcat tgttgcggaa atctagatag
    57601 acacccgcgc cttttagccc ttcttttttc aaaaacggtt ctctttccaa tacttcttct
    57661 ggtgtcagcg tataattctc atatttggtt cctgtcacat ttgccagttt gtcataaagg
    57721 tccattgcta ctttgaccga gaacatattg aaggttgttg ctccttcgcc ctcatagatt
    57781 ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa tactttgtac gactgcacgt
    57841 tcaccaactg tgtcagctac tacttccaca tcaaatgtct tcagataacg aataccgcca
    57901 tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat
    57961 cctgttttga ttcctgatgc tgctgactgg actgccacac cggcaccagt gattccgcca
    58021 ccgatgatca acagatccaa ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc
    58081 ttgtttgaaa acatatggat ttcctcctta ttcaaatgct tgggtagctt ttactgcttt
    58141 tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc atttgaactt caaatagctt
    58201 gccagcttca taaaattcgc ggatctcgtc tgtatctttc caataaccga cagctaatcc
    58261 tgctaaaaat gctgccccta gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac
    58321 attcaagata tctgtttgga attgcatcag atattcattg tttgctgcac cgccatctac
    58381 tttcaataca ggaatcttga ttccagtgtc atcttgcatc gtatcgatga tatctctcac
    58441 ttgataagca atcgactgta aagtagcttt aatgatatct tcttttgtcg taccacgtgt
    58501 caagccaaac atagcccctc gtgctgcctg atcccaataa ggagcaccta gcccaacgaa
    58561 tgcaggaaca acataaacct catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc
    58621 gctagctttt tgcaacattt gaagtccatc tctcagccat tgaatcgaag aaccagccac
    58681 aaaaatactt ccttctaaag catagtaaac ctttccattt atgccgtaac caattgtcgt
    58741 caataaatta tttttggata attgcggttt ttcaccggta ttcatgacaa taaatgaacc
    58801 tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc
    58861 ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt ccataaaaat gataaccttg
    58921 cgtataacca taaacttctg agttagaagt gacttttggt aacatcactc gaggaatatt
    58981 tagtaaatct aaaatctcct gatcccaatc tagctgatgg atattgaaca acatcgtccg
    59041 actggcattc gagtaatcag taacatgtgc ttgtccatcg gtcagtttcc atactaacca
    59101 agaatcgatc gttccaaaca tcaattcgcc tttttcagct cgttcttgcg cgccttctac
    59161 atgatcaaga atccagcgta tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt
    59221 tttttcgtgg aaaaattctt gatagccttc attttttaag ttatctgcaa ttccagctga
    59281 ctgtctggat tgccaaacaa ctgcatgata gattggacgt cctgtatctt tttcccatat
    59341 tactgttgtt tcacgctggt tcgttatgcc aatcccagca atctgatttg gtttgatccc
    59401 tgattcgatg aacgctccgg caataactga ctgtacagaa ttccagattt catttgcatc
    59461 atgttccacc caactttctt gcgggaaata ttgcgtgaac tctttttgag aactgccgat
    59521 gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta gttgtacctt ggtcaatcgc
    59581 catgatatac gttgattctg tcattgtgat tccctccatt atatgcaaac gctttcttta
    59641 actactctta tcatactact gttttaaaaa aagaaactgt caaaatttta ttcttttttt
    59701 ctttttttga cagtttttta ttatgaagct attttttaat caattctttt cttttttcag
    59761 cgtctctatt cttcttttca atcgaataat gtcgtaaggt gacgcaaatt ctgacagata
    59821 atattcacac tgttttttgt ttctctggca aaatgtgatc accaaatcat attctttacc
    59881 ttcttcataa cttccaatct caataccgcc gattcccctc agttctctga tcagatattg
    59941 ttgaaaagca atacgataaa tcggcagact ttctaaatcg attccaatca ctactggttt
    60001 tgcaatataa aaatcgatca tcaaaagaac attggcatac tggatcatca ggtaactaag
    60061 ctcactatct tcagctcttt tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc
    60121 cagtaactgt gttaaggaat gcccgagcat tttctgctgc tgttccaaaa gccgatcacg
    60181 ctcatagact tcgatctttc caacaaagaa aaaccattca ttgttgatct gggaaatttg
    60241 gtagcctact gccttttctt ctttgatttt caaacgacga ggacgataat gccataacat
    60301 cgtttcccga atgtaagtat ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc
    60361 atactgataa aatatttctt cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat
    60421 cattgtttcg tatacattca gttctgctgc tgttcgacta agatgcatca cgatgattgg
    60481 atcgattgct tgataaagcc ggtccgactg atagagtaat ttcttttctt tcaaagttgc
    60541 gtacgtactt ttttcattca atagtctttt tttcgtaatc ccgagccagc aagcaatctg
    60601 tgcttctgct gatgccgtaa acgttgtttc taatacacgg tccaatccta gaatgaacgg
    60661 tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga tcttccggga tatattggaa
    60721 tagttcataa taaaaatatc gtatctgtag ctcttctccg tataattgac cgtttttgat
    60781 ttgtaatcca aattcctcta agagctggtt caattctttg atctttcgaa aaacagaaga
    60841 ctcactgata ttaaacgctg tcgctaatcg aacgatggat acttgctgat gttctaataa
    60901 atagacgagc atcttaaact tcaagctttc ctgcaataaa aacaatagaa ttttttccaa
    60961 acttacatct tcgtccaaca caagcagcgc ctgatactca tttcgctgta cttctacttt
    61021 tttttccatc attcggccaa tcaattcaat atcttccaaa tattgatcaa acgaggcttt
    61081 tgacagttgg acatgctctc tcaaaccatt gatagaggct gttccccctt gcagaacaag
    61141 gtttcgcaaa atcaggatct gtaactgctc ttttctttct aataatgctt caatcttcat
    61201 aggcagtatt cgccaccttt gaccacacca tatctggcac acggtattct ggctggaaaa
    61261 atagctcatg ttcttctaac tgttcaaacg tccatccttc ttctactgca attgtcagtt
    61321 ggtcaagaag gtggaacatc tcttttttgc tttggagctg tgctccaaga atcttttttt
    61381 cagaatcaaa tagaaatttg agtttaactt tgctttgatt tattgctgta agcttgtagt
    61441 ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct ctcttcttct gtcaaaccta
    61501 cgcttcctaa gaaatagcca aaccactgat tgccgactgg acgataggtt ccctgatcct
    61561 ttgtctgtga gccactaatt gtcttgctga catttcgtgc tgtacggaca gcgttattta
    61621 ctagagaaac atacattttt tcatttgttg gacgaaaagt tacttggatc gcatcgccaa
    61681 tcgcataaac gtctttttga gacgtttgca agtagtcatc tacccagatc gttccatcat
    61741 cattcaatgt taattgttct tcccacatct gattgtcggg acgagaatga ttagccagca
    61801 aaacaagatc atagttctcc tttctttttt ccgtctctac aatagcggtt tcattttcgg
    61861 tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc ttgatttttc atttccttta
    61921 ctaacggttc tgtcatttca ggatctagat aacgaaaaag aattgacttt cggctttcgt
    61981 aaagatggat ttgttttttt cttgatgcca acccttcagc tatttcgatt cctgcttgtc
    62041 ctgcaccaat aattgcaatt ttctttgcat tcatgatctt ttgctgaaga gccgttacgt
    62101 tttgtatctt tttaactggg tgaataagcg ttgattctgc agaaatattt cgaaactttt
    62161 gatttgaacc agtcgctaga atcaaacgat caaaatccaa tttttttcct cctgctaaaa
    62221 cgactttttt ttctttcaat cctattactg tcttttctgt gtaaacatgg attcgatagc
    62281 ggtcagtcag ttcttctttc gtgatccaag aaagttgttt ttcactggta acctttcctt
    62341 ttaatatcaa attgaacccg ctgggaatcc atcctaactt cgattgcttt tcaatcaaat
    62401 atatttcact tgttggcatc aattggcgaa gataaatagc cgcatgaatc cctgcaaacg
    62461 acccgccgac aattacaatg ttcatctgca ttcctctttc ttataaggtg ttctactgct
    62521 aacttctcaa tcagaaaaca cagtattcat aactcgcttt caaccttatg atacatagcg
    62581 atgcagcaga atcaaccctc tccatcatac cgtgttctag gtctgcttct caataatgta
    62641 ctagtggacc taattgaaga gtatcatttc tgaagagatg tttcaaaaaa tgtactctta
    62701 taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa taggaaacag aaaagaacga
    62761 aaagacgagt tttagcgtgt gtaatgtaat agttagaata gacacatcat ttatttttaa
    62821 ttctttaatg cttcaacaac tgcttcgcct aattgatgag cagattgagg attttgccct
    62881 gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag ctgctccaga ctgatagttt
    62941 gctccttttt ctttcaaagc atcttctgct aagaatggta cttcattcgt tgtaccattt
    63001 gcttcctctt cttcattact gaacccagtc actgtacgtc cagctaaaat agattttcca
    63061 gactcatctg taactggcaa caatcctaca acaccgtgac aaacagctga gaccagccct
    63121 tcatttcgat agatttgtga agcgactttc ccaagatcta ttgcttctgg aaaatcccac
    63181 attgttccgt ggccaccagc ataataaacc gctgcataat catttgcatc cacatcatca
    63241 ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt catcttcata ataggcccaa
    63301 tcgatttcat ccatattcaa aacactttgc ggttctaaag gtacatatcc gcctttagga
    63361 ctgacaaaat ctgcatcgat cccatttttt actaatacat cataaaaatg agtcagttca
    63421 cttaaccaca aacctgtagg acgctcgatc ttttcgtact tttctacatt tgttaatacg
    63481 atcaatactt tcattttccc atcctctttc tataaaaatt gcgttattga taacgtgctt
    63541 tcaagaaatt atataatggg cttggcaaat aaagctcttt atatgctttt tcaaaaggtt
    63601 tcgttacaaa ttcatcctca cggttttcca ataatttctc tgcccagtca ggatcgacga
    63661 tcatttgctg tccaattgct gcaagttctg catatttcaa tacctgctct acatcttcgc
    63721 gattgcggat cccaccgaca ccaactaaag ggatctttcc ttgaatcgtg ttatggacca
    63781 cttcaagaat cgatttatcg ttgtatttat cagaacgagc tattcgatca tacatattta
    63841 atgaaacatg cagataatct agttttgact cacgtaatcg ttcaagaagc cagatcgtgt
    63901 cttcaaaacg gatacctggt gtttcatatt cttctggtga gaaacgatac cctacgatga
    63961 atggtttttt agcatattgt tcaaccgctg agaaaaccgt tgttaataat tcttcaataa
    64021 aacgataacg attttccaag ctaccgcccc attgatcctc tcgtctattc gaatgcggag
    64081 agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg
    64141 attcaatcgc tctacgtgtg gctttgccaa atgcttggat tgtttcaacg atttcttctt
    64201 cactcattgc tcgtggtgtt tccgcatcag gacgttttgc tgcaactgca cttgcagata
    64261 caggctgttc tccagataaa gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa
    64321 taatcgcttt tgcgccttct ccttggattg ctttcgctag ctcacttaat cgaggaatca
    64381 tttcatcatg agcgatgctg agttcacctg gccagccttt tccgatatct tggacatttg
    64441 cagcaccagt aataacagca ccaagacctt tagaacgttg tttataataa gtgatctcat
    64501 cttcggtgat cactccattg aaaaagctga gttgtgtcgt cataggcgac agcatcaatc
    64561 ggttcttcaa ttgaactcct gaaggtaaag taattgattg atcaaattgt gacattgtca
    64621 ttcctcccat aattcgctct gattaaccta tcgagaatca tctatcatta aaccactcga
    64681 ttagttgtac atgcaactaa tttgcttaaa aaattttatt ttttttgtaa cttatctgtt
    64741 gcatcattca ttgtctgggc taattcttta cttgcttcat ttcccaaaac tgctttgtat
    64801 gactgatgaa gttcttccca gctaccatcg acttccttag caaattggcg tccttgttct
    64861 gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt tgacgtatcc tttatattct
    64921 aatttttcaa caaaacgagc aatcgttgat ggggcggaat agagcatttg tgcaatatct
    64981 ttttgtggta ttccatcttt ttcttttaat atcaataaaa taaaaccata agttggtgcc
    65041 agacctgtcc ttctaaacgc ttcatcagct aatttattca acatgcgatc tagctttttc
    65101 acgctaaaga aaagacattc atctaacgct ttttcaattg gcttttccat ttcatcccct
    65161 ccattggcgt acatatcata gtatgatttt tattttttgt caaaaaacac ggaataatgt
    65221 ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg
    65281 ttttcgacaa aactccttct ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt
    65341 tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
    65401 taataccgaa aaactggctt ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc
    65461 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttgt cgttttttga
    65521 gctaatgccg atggtcagct gattgtacac cgtttattga gaggtcgtga gcctgccgtt
    65581 gaactccgag tcacaaggag caatttcaaa aaacagcttc tcatgtttta tgactatggt
    65641 gacttgtgat cgaggagttg gcaggagaat actgtttatt cgtaaagaag gggctgtgac
    65701 aagacttttg tcacagtccc ttatttcctt ttattatagg atttcgccat ttgattggat
    65761 gacttgtctg taccaatcaa atgatttttt ctttaaccgt ttctttgtgc cattcccttc
    65821 gtcatcttga tcaacataga tatatccata acgttttgac atttgtgaag tagaagcgct
    65881 gacgatatca atgcagcccc acgacgtata accaaataaa tctactccat ctttgatagc
    65941 ttccttcatc tgctcaatat gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt
    66001 tccatcttct tctactttgt ctatagctcc gagtccgttt tcagctatga acagtggttt
    66061 ttgataccta tcatataatt gattcaaagc aattctcaaa cccttaggat caatctgcca
    66121 gccccattca cttgatggaa gatacggatt tttaattcct ccagttatta agtttccttg
    66181 gaccccttcc caattttctg gatgagcact gactgcacta gacatgtagt agctaaaaga
    66241 aacaaaatca acagggtagg ctgctaagat ttcttcatca cctacttcta tttttaagtt
    66301 gacacctttt tcttcaaaca ttcgttgcgt atacgctgga taatatcctc ttgcttgaac
    66361 atcactaaag aacaaagaag ttctttgctg ctcaattgtt ttttgaatat catccggatc
    66421 acaagaatac ggaacaagaa gctgatagct gatcatacaa ccgacttgtg aacctttgat
    66481 aatttcatga caagcttttg tagctaaagc acttgctata aactgatggt gtgcaccttg
    66541 gtatttcgct tgttccaaat ggggatggtt ttcttcaatt actcctacgc taacaaaagg
    66601 gtgatgtttt acacagttga tctcattgaa agtcatccaa tattttactt tatttttata
    66661 gcgtttgaat actgtttcag cgaaacgaac gtaaaactga ataagctttc gattaggcca
    66721 tccaccatat tctgtaacca aatataacgg catttcataa tgggaaagag aaacgactgg
    66781 ctcaatccca tattttagac attcgtcaaa cacttgatca taaaaaaata atcccgcttc
    66841 attgggtgtt tcttcatcac catgaggaaa aattcttgtc catgcgatag acatccgata
    66901 gcatttaaaa cccatttcag caaataaacg gatgtcttct ttaaagtgat gatagaaatc
    66961 atttccgtgt cttttaggat aataatactt gctgttggga tcctgcgccg cttttacttg
    67021 ctgatgcgtc atcatatgcc attgatctgt ccatttttct ttaggtaaat cgctgtcgaa
    67081 tgtatacaca tcagacacag ataatccttt ttcatctaca tcccatgcac cttcacattg
    67141 attggccgct gtcgctccgc cccataaaaa tccttctgga aattttttca tatcgatccc
    67201 cctaaacttg ctagttttct tgtttgatta tttttcttat gactagtaat gatatttttg
    67261 aatctactac tatttgtaac aataaccatt gtggtcggat catatccctc tttgattaat
    67321 tcttgcaaat ctacttccat caataaatcg cctttacgta ctttttttcc atcagatact
    67381 gcactttgaa agtatttgcc atttaaattc actgtatcaa taccgatatg gatcagtatc
    67441 tctgctcctt cttcactgac taccccaatt gcatgtttcg ttggaaaaac tgttgaaact
    67501 tccccgttta cagaactgta gatttttccg tccgttggaa tgatcgcaaa cccttctcct
    67561 aaagatccac tagcaaatgt tggatcgttt acttcagata aatcaattat ttcacctgct
    67621 gcaggagtac ttaacactat atcctttgag gattcttctt cgatttcatc cttgtagccg
    67681 aaaagaaaag ttagcataca ggaactgatc atcgctaaga aataactagc aactagccaa
    67741 acaaatcctt caccaatata aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa
    67801 gcaccggctc ctgcggcacc agctactcca ccaaatatag ctccacatat acaagcaatg
    67861 taaaatggtc gtttatattt aagagtcaca ccataaacag caggttcagt gattccaaat
    67921 aatgctgtga taaatgcaga taaagataaa ctttttactt tcttattttt cgttttcaaa
    67981 aataccccaa gtactgctcc tgcttgcgca gctacggcag tcatacatgc tggacctagc
    68041 gtatcacgac cgtagaaaga aagattgttg gtcattactg gtacaagtcc ccaatgtaac
    68101 ccaaaaatca ctaacacttg ccaaaatccg ccgataacag caccagataa aacagggcta
    68161 aagttataaa cagctaaata tccagctgca atcagctgac ttgcataatc tgcaattgga
    68221 cctacaacta ataaaataat tgggaccatc acgatgatac aaatgaatgg tgtcaataaa
    68281 tttcgaatac tttcgtgcaa gcttttgcga atcttaggct caatataaga aagaacccat
    68341 acagctaaaa ttgctggaat aaccgtactt gtataccttg ctagaatcac gggcatccct
    68401 agaaatcgca aggttatgct atcactatat gcactgacaa tcgtaggata aacgagagct
    68461 gctgcaataa ccatagcgat gaaacgatct gtattgaact tttttgcagc agtatatgcc
    68521 aaaaatatag gcaagaaata gaaaaatgca tcagctgctg cattcaaaat gatataagtt
    68581 cctgattcag tagttaataa attcaaactc gtacataagg cgagtactcc tttgatcaaa
    68641 ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag cggcgatcat gtctataaaa
    68701 gagtttaaaa tgcctttttt ctcagttcct ctctttccac tagatgcatc atcttctagt
    68761 ttcatttgtt tggataatac gtcatacact tcatttactg catttcctac tacgacttga
    68821 aacattcctc cagcttcgac tacagtgatc acaccttcta gttgctctac cttctcctta
    68881 tctgccttct tattatcttt tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg
    68941 ctgacatttt cttcgcctcc gacaaatcgg acaatatctt tcgctaattg tgaatagtcc
    69001 atttttactt cccccttgtt aattaatgcc gtagcaatcc ttcttacaag ataagtatag
    69061 ttcttagaaa acggtttctc cattcaatta gtaccccttc taatagcgaa cgaaactgta
    69121 caatagtgtg tatggtttac ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt
    69181 atatcgtttc tcttatagtc aaaggagaca aaaaaaacac gcttgatctt cctttgctga
    69241 tgtataagtg gtatctcaag ctgatactct gtgatttttg tttctacttg gcttttgata
    69301 agacagcaca acaaagtctc ggcatttaac ggaagcataa ctaggtcaga actaggagag
    69361 attagttgct gaatggaatc tatcttttta tggattttta tttgattttc ttctttgtag
    69421 taatgatcaa gcagttttgt tttctttttt tcttcaacta tggtcagctt ttcacgcaaa
    69481 atctgataaa tctcccaaga ttcaaactca tctagaacac taggtagata aatcaaatca
    69541 tcccactcaa aaataagctc ttgctcagta gtcaaaattg catcatattc cgttattttg
    69601 tttgattgat ttttgaaaag atacactggt tctatccggc attctttgat ccacatgttc
    69661 atactttgtt gaatctgttt ttttagtgta ttcaaaatac taattggctg gttactaaca
    69721 atcagtatct ccccttgttt tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa
    69781 taagcgcttt cagctaaatt gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt
    69841 aaataaattt ctaaaggata acgggagatg atcttttgta caaaatgatt catggtatta
    69901 tgtccggcat ccatcctcgt tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca
    69961 tggaacaata acgaaacagg aagatctaaa aaacgaataa tcgtttgctc aaatgctctt
    70021 agattcttct ctatttcttt gtttttttca tcattaatca tcaaataatt taactctaat
    70081 agtcgatttt caattgcttg tttttcttga ttggaaaaag aatagggtag ttctgatgat
    70141 aacttttttg tgagggtctc taccatcgga ataaataatg tatacctgtt tgttttttct
    70201 aaaagttctt ctcttcgaag aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc
    70261 tctcctgaaa caatataatt atatgatata agaacctctt ttaatgtctt ttggacagtc
    70321 ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa accttgcgtg ttggatcacc
    70381 gtttctgttc caacaagaga taagaaaatt ctttttgaat tttcttcagc aatgattttt
    70441 agtccttttg tcgacgaaac ttccagttct aatttaggat tgcgctcgat ccaacgcaaa
    70501 atcgttttta tttgtaatga tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt
    70561 tgcatactga catacccttt ttgaaacaat aagtacgtca caatagttga agctcggaaa
    70621 tccatgctgg aatagatttt atgtttcgtc acctcttgta tgatttgctg tttagtttca
    70681 gattcaattg attctaaaaa ataacctgaa cgtttatttg actggataat gattttcttc
    70741 tcgtagatag caagcatatt tttgatttca gtttggatcg ttttcttgtt ggtacctaaa
    70801 gcttcagcta attcttcgct tgttatccaa tgattcttat ttcttaatac tacaagaatt
    70861 tggagttgtc gtttatttaa gtccatttgc ttacacctcc tctttgttat aaacatacag
    70921 gaaaaacctc tcaaatgaca atagtcactt gcagaggttt aaaaatcatt tgaagaaaga
    70981 tctatattca aggtttttac cattagactg gataatttgt ttataatatt caaaactatc
    71041 ttttttcaat ctcctaaagc ttccgctccc atcattttgt ctatccacat aaataaagcc
    71101 atatcgcttc ctcatttccc ctgttcctgc cgatacgata tcgattggtc cccaccataa
    71161 gtaaccaatg atttggcaac catcttttat tgcctcagca atttcctgaa gatgtatttt
    71221 tagatactca caacgttttg gatcgtggat tcgattcaat tcatctggtg cttcatctag
    71281 tccaatgcca ttttcaacta tgaataatgg taattcataa cggtctgtca attcattcat
    71341 gacataacga aaccctagag gatcaattgg ccaactccaa ggttcgggag atttttcctt
    71401 caaaaaggga ttatcgaaac catttgctcc gccagtatct acgcgcattt ctgcttcttt
    71461 tttatagaca gcgctacgat aataactaaa tgcaatataa tctgccgtgt tagcttgcaa
    71521 gcactgttcg tctccatcta acattactgg tgcacagtct tcttccttcc atgtcctgta
    71581 aatataagct ggataatgcc ctcggcacat gacatcggag aaaaaccatg aattgcgttt
    71641 aaattgcagt gaaccaaata cattctctgg atcacagtca tatgcataag tcgctaatgc
    71701 agaaaagcta cacataatac ccatcttagc atttggatcg attttcttta ataattgagt
    71761 agcacgggcg ttcgctagaa acatatgatg acaggcttga taaatcactt tttttgttag
    71821 tgtagcaatg ggttcaagtt tatctctagc agggaatgta accaatccag cagctgcata
    71881 aggcatacga aaagcattat tgatctcatt aaaggtcatc caatatttca ctttcccttg
    71941 gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg
    72001 ccacccccca tactctccta ccaatgctaa gggggtttca taatgactaa gagtaattac
    72061 tggctcgata tcatatcgat ttaatgtatt gattagtctg tcataaaata cgagaccagc
    72121 ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg
    72181 aaaagctttt aaacctaact ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa
    72241 atcaatcgct tcatgagaaa gataatggag tgattcgtct aaagcaatct cccaaatctt
    72301 tttttcttca ttccactgaa tactaggatg ctggttcatg atcccgataa gcgtatctgt
    72361 cacattaggt aatttgccgt cttccaacca tgcaccttca cattgattag cagctactgc
    72421 tccgccccat aaaaaatttt ctggaaatat ttcctgatcc ataactatct ctcctcgcta
    72481 tcaacaattt cttacttgaa gtatagctcg attcatcaaa acaagatatt ctaactattt
    72541 cggcagaaga gaagaaaaaa attgaatgct actcttcatt tttaatattt atatataaca
    72601 aatacataag acgatccccg aaagaaaaca ctcgttaacc atctaagcaa acgatttatt
    72661 ttacttccaa cacaaatgct tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt
    72721 tcagaggata atttgacaca ataaccctta aagcttcacg tggaatagtg aacgtctgct
    72781 tttcactgga catattacaa ataacgagcc attgctggtc ttcatatatg cgagaatagg
    72841 caaagacatg ctcaggcgtc ttttccacta attcatagtc tccccagacg atgatgtcat
    72901 gttttctacg caaatcgatc aatttcttgt acatataaaa aacagaatct tgatctgcta
    72961 acgcttcttc tgcattgatt tctttataat ttggattgac tcgaagccat ggaattccag
    73021 tagtaaaccc agcatgttca cgggcgttcc attgcatagg cgtcctagca ttgtctcttc
    73081 ctttcgcctt gattgattcc aagatttcct cttttgtaaa tccagaagaa atcctttcgt
    73141 tatacatatt gattgtctca atatcttttg cttccgcgat atcatcgatt gggcaattcg
    73201 tcatgcctaa ctcttctcct tgatagatat aaggggtgcc tttcatcata tggagcaaga
    73261 tagcaaacat cttggcactt tcttttcgat acacatcgtc atttccccaa cgagaaacaa
    73321 ttcgcggaag atcgtgatta ttccaaaata aactattcca tccttcatca cctaaagatg
    73381 tttgccattt ggataatact tttttcagtt cagagatact caagggtttc aaatcccatt
    73441 tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa ttggaagacc atggataatt
    73501 catttctttt aggattggaa taaagtttag cgatttcggt agttgctccc catgtttcgc
    73561 caacagtcat cagatttttg tcaccaaaag ttgcttgatt catttcctga agatactcat
    73621 gcagtttagg tccattactt gtgatttctt tatccggttc tttgccaatc atatcgatca
    73681 cgtccatacg aaagccccca atacctttat cgatccagaa attcatcatt tgataaatct
    73741 cttggcgaac ctgtttattt ttccaattca aatcaggttg acgcttgcta tacaagtgca
    73801 gaaaatattg ccccgttttt tcatccaact gccaagcaga accactgaat attgagcgca
    73861 atctattagg ctcttttcca ttgaccggat cacgccaaat ataatagtcc cgataaggat
    73921 tttctttatt tttacgggcc tcaatgaacc acggatgttc atcagaagta tgattcacca
    73981 ccagatccat gatgatacgg atatttcttt tctttgcttc ttcaatcagt tcttccattt
    74041 cttccatcgt accgaattct gccatgattg cttgataatt actgatatca tacccattgt
    74101 catcattcgg agactcgtat actggagaaa gccaaattgc atcaatacct aaatacgcca
    74161 gataatccaa cctggaaata atcccccgca aatctccaat accatcttga tttgaatctt
    74221 gaaaacttct tggatagatt tgataaacaa ctgaatggtg ccaccatttt tgttccatga
    74281 ttttcctcct ataaatggat gtttctattt tttgcttaat catttatttt agtctctctc
    74341 actaaaaatt gtaacgcttt caaatagcta ttgcaccttg ttatcggtaa cagaataaaa
    74401 caaaaaaaca gctgaggaaa ttccctcaac tgttttttga aaggattgat ttatgcaggg
    74461 aagaatgctg atttcacaac ttctttataa acggtgttca ctcagctgtc ccttggtatt
    74521 agctcaaaaa acggcaaaat ataagaaact attttttcgt ttttttctct aatactcagg
    74581 ccagaacgct gatttcacaa cctctttatt tcacattcaa tatccacatg aatcccacaa
    74641 atacaaagaa cagaccatac atgattggag atacttcttt tcctctttta gctgctaaca
    74701 tggtaattgg atagaagata aaacctaatg caatcccatc tgaaatacta taagtcaaag
    74761 gcatgccgat caagatcaag aatgaaggga tcgcaatctc taagtttccc cattcgattt
    74821 gtcgcaaaga ttgtgccatc aatactccta ccataatcaa tgctggtgca gttacttgtg
    74881 aagtaacaac tgatagtaat ggagaaaaga acaagcctaa caagaagaaa atccctgtag
    74941 taatagctgt gatacctgaa cgtcctccaa cggcgatacc agctgatgat tctacaaatg
    75001 caccaacagg agaagtacca aataaggaac ccgcaagcat agctgttgaa tcagaagcta
    75061 aagctttccc tacacgaggc atcttgttgt ctttcatgaa acctgcttgg tttgctaatc
    75121 caactaaagt tcctgctgta tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc
    75181 acatttgaag agaattgatg tcccctacat gttttaatgc aactaaaaat gttggtttca
    75241 aacttggtgc tgcggcaaca atttcagaag gcattggaat caaacctgtg accagaccta
    75301 aaatcgttgt agctgccatc ccgatgaaga tgccacctgg tacacgccga acaagcaaga
    75361 tagcagttac gactaatcca aaaatcgtca accaagtaga accaacgttg agtgatccta
    75421 atcctactaa tgtagattca ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag
    75481 cgataaatag accgataccg ccagaaatcg catatttcaa atctgctggg atcgcatcaa
    75541 tgatcatttc ccgcagcttg aagattgtga tcaaaataaa aatcaaagaa gcaacgaata
    75601 cgccggctaa tgctgtttcc catggaatcc ccattcctaa acaaacagaa taagcaaaaa
    75661 aagcattgat cccaagagca ggtgccgttc caattggata acgggctaat acccccatta
    75721 aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa cactgctcct tcatccattc
    75781 cagaagcacc taatacagtt ggatttacaa ataagatata agccattgag ataaatgttg
    75841 taaatcctgc cagtacctca cgttttaccg tggtgttcaa tttttctatt tcgaagtagg
    75901 aaagtatttt ttccttcatc ttccatcccc ctaaatccga acattataaa taaagtccgt
    75961 taaaatttcg atttattaat aaaacaacgt gttttatcat atactaaaac aaaacataag
    76021 taaacaaaaa attcggattt taagcaaaat ccgaacgttc catctgatta aatcccttta
    76081 tctcaaagtt catgaacatt cactttggat tacaagaatt tctttattgt gttaataaag
    76141 tttcttcatt ctagtctcaa aaatcaatag tgtgatttga ttttaccatt tcgtttctgt
    76201 tcttctttga tgaattcttt tgtatcgaca ggattcagat tatttagtcc gccaaaacct
    76261 gtatcgacca tttttcccca gccttttgtc ttcttttttc ctgatttgag attctttgct
    76321 cgaagcttct cactatgttt tgctcgccga tacttcaaat aaccttctaa tcttcccatt
    76381 agatccactg tttttcctcc tttttattca atcagacgac tcagcttttc ctataacaaa
    76441 ataattgcct tcagggtcag aaaaattgaa agtcaagatg ccattgttat ctgtgatttc
    76501 tcctggttct tccatgttct cgtagatagt ttcaaattca tccgtaaaaa atactaaaga
    76561 aggcggtggt cctaatacct ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc
    76621 taattctaat tcttttgaga tgctgagtac aatactctta aattcttctg gcaattcgat
    76681 tgtttcagaa atttctgcac caattttttc tacaaagaat cggcaaatca tctctacgtc
    76741 attgacataa agcatcacac gtgttttttg tatcatcatt tgacccttct ttctatttga
    76801 ttttctttcc tgtctataaa ctgattccat tatatcacga aagcgcaaac accttgtgtt
    76861 tgatactatt caagaaaaat aaagcaatag tttctaggat tgctttcctc cacaaacttt
    76921 ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta tttcgattct tttataattc
    76981 tgcttctgat tcatctttgg ttagtgtgtg tctaacacta gttacgccaa atgtttgatc
    77041 gattttttcc ttcaacgctt cgattgcctg tgcgccacgt gccgcttgtt cagcattgat
    77101 ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc
    77161 acgccagttg agacagcgac agatcgctcc ttgttcatca tagtagatga tttcttccgg
    77221 aagtgccgga gcatcggaat ctgcacctaa aggcaggaat gattcgccac cttgggcttt
    77281 tcctaaatgc agatttcctg cgatcttttc gatatcttct ccgccgcaag gtacgccaaa
    77341 agacaaggaa atactattat agatatctac aagcggattg attgggaaaa attcccgttc
    77401 ttgatttgca cgtttcagta aagcttcaat agaagaacgt gcaccttttt tagtcttgaa
    77461 tgtggtaaat gcctgacgcc actgagcaat gacttcgttt tggctgaatg tttcttctgt
    77521 aaggtaattt cttgcttctt ttgctgcctc attcaataat tgatgatacg tttcttcact
    77581 ttcttttgtt acgtggttat cgataccatt tacggataag atttcgatgg ttgcttcagg
    77641 aaatacttcc cagaaattgg tatccactat tacttttttc atctgttcac acctcttgat
    77701 tagaataatt gcttgatgaa tagatagtct gtttggggat catctcccat ataaaaaaca
    77761 tgttcgctgt atttgataaa tcctttttta cgataaaaag cgagcgcaac atcgttgtgt
    77821 tcccaaacac cgagccaaat ggctgttttt tgcttttctt ttgctttttc aatagctgta
    77881 tctagtaaaa atgtaccgat accttgatgt ttaaattttt ttcgtacata gattcgctcg
    77941 atttctaaaa aatgatcagc catttcctca gattgactcg ttcctgtatt tagtttcagg
    78001 taacccgcag gctcttgatc gatactcact aggaaaaaat cggtttcttt atcttttagc
    78061 tcttctgtaa gtttttctag tgcataagct tgtgtcaagt aatcagctaa atgctccggt
    78121 gaattatctt ttccaaatgt atctgtaaat gtttcgatac taatttgttg tagtaagtct
    78181 acatctgaag gcgtcatttt tcgaatttcg tatttattca cggcttcttc ctttcaatag
    78241 atccttttat tgcctttttt gacaaaattc caatcttcgc taacattctt gcatacttta
    78301 tttaaaagat attccaattg ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca
    78361 ttggagtagt tgttttctcg aatgatgaaa ggatagattt ctgctccttt tttagtcgga
    78421 tagagtttct tgattttttt attttcttta tcttccaaac gttcgatcat cccattggat
    78481 tctaatttct tgattgctcg ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt
    78541 ttttcttgga tgattccagg attttcgcaa atacgtacta aataaagata ctgtcctctt
    78601 gttaaatcga cttctttgaa ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt
    78661 ccgatgtctc gaaggatttc agccatttca tcccaccttt attttgtttg taagactagt
    78721 tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa aaatcaagtt cttttttaat
    78781 aactagtaaa gtatattctt ttcttctttt acgaaaaagc tcaatcacaa tagatctggt
    78841 ttttgttttc taaaataagt aactgtttct tcgttgttcg cttttctgat agctttggca
    78901 actcttctat actaaaaaat cccatgttgg aagtttcatt attctcaata aacttggcct
    78961 cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat
    79021 ctttttgttt attcgtatca aaaactgctc gcaactgagt agtttctact gttaatcctg
    79081 tttcttcata gacttcttta cgcacatttt cttcaggaga caatcctatt tcagcaaatc
    79141 caccaggaag tgaccattct ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt
    79201 ttttgatata ggcacgaaca tctacttttg gtgttggatg tccttctcca atcgttgtga
    79261 gcgcttttat ttcttcgaaa gtttcttttc ctatttcact gatcagttcc aatgaaatcg
    79321 tccggatctc ctcaaaacgc tctttatcaa atacatcttt tccataatat agaccagctt
    79381 gagaaagtgc gagtagtctt ttgtattgag ccaacttctt gttcattctc ttcccttctt
    79441 ttttagcaaa tgtatgacta tgtaatcaag tatagaagac tggtttaatt acggcaaata
    79501 aatataatga aatctgcaat aaataaaaga atgttaaaaa actcgagaac aaaagacatt
    79561 cgacttctct ggggctgtga ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc
    79621 ccaccaaacg aaaaatatga cgagctattc ttgtctggct gttcttatta attgaggcag
    79681 gacagctttt tcacaacctc cccactaaac ggtgttctcc taccaactcc tcggtcacaa
    79741 gtcacaatat tcatcaaaca tgagaagctg tttttttgaa attgctcctt gtgactcgga
    79801 gctgaacgac aggctcacga cctctcggta aacagcgttc aaaagccaat ttttcgatat
    79861 taagccattt ctttgttact tattgtcatt gatttctttt tctaatttat ccaatagtgg
    79921 agtgtaatcc acttgagtat agtctgttac atcaaattca tatagtgttc ctaactggaa
    79981 tttgtcatat tttcggtcgg cgatgatctt tttgaatgct tctttcgtaa tatgattggt
    80041 ccctttcgtt cgatcttcca atacgtcacc atagtgataa tgatccatga tatagctcaa
    80101 atgacgatct gattcacgat cacgtttgta ccgacgttgc cataacactt caaagtctgc
    80161 tgttaggcga atcgtgatca cctgatatcc gtatctatct gctaattctt ggagtcgtgc
    80221 tttttgctta tcactaaaag gatactcgga cacaatcact ttctttcctg cttccatata
    80281 caaatcaagt gctttgtaat agaaaggata aactttcgtc ctttcttgtt ccgcccgttc
    80341 ttccaaattg ttaaacccga tggattctgc atagtattcc ttgatttcat cgagcgtgat
    80401 ggcatacata tctggaaatc gttcccgtag tttattcata agatatgttt ttcctgtacc
    80461 aggactacca gccagaagaa tgaaatactt tttcatctca tttatttcct aagttaattg
    80521 gactttccaa gttcataaac agtccaatct tatgcttgac aatttctttt actgcttcat
    80581 ttgctggaat cacattatgg cgaagagact tgttgatttc tgtttcacta aaggtttctt
    80641 tagctacttt cgtccagttg actaaaagct ccgttccaac attgaatttg cggatcccgt
    80701 tattgatcaa ttcaggataa tcttcttctt tcacacctgt tcctccatgg ataactaggg
    80761 ggacatcaac tactgcatcg atttctttca gcaagggaat gtttacttct gtttttgatt
    80821 tgaattgtcc atgattcgta ccgatagcaa tagctaatgc atccacgcct gtagcttcta
    80881 caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc attttcagcg acatggattc
    80941 cttcttctgt accgccgatt gttccaagtt ctccttctac ggaaacaccg tgcgcatggg
    81001 catattctac tactgctttt gtcttcagga tattttcttt gaaaggcaga tgggacccat
    81061 catacatgac agaagtatat ccgctgtcga ttgcttcttt gatatcatca aagtctcggg
    81121 cgtggtctaa gtgcaagact gcatcaacca tttcttcttc tgcaatagct gtcattacat
    81181 tgactaacac atgcatgccg atatatttag cggtatcgac acttgtttga ataatgatcg
    81241 gcgaacccat ttcttttgct gcgcctagca tagcaggaag catttctagg ttatgcgtat
    81301 taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt tgttacttct ttcaatgttg
    81361 tatacatctt ttattgccac cttatctttt tatttgtttc ctttgatcgt tacttgtttt
    81421 gcaatttgtc gcatctcatc cattttgccc atgtagtaat cgatcccttg atcatcacca
    81481 gcacgagcag catctgcacg cttctggtta tagaggctca tcagtttttt atacccatct
    81541 ccgatcgtct gtttattttc caaactcttt tctaaatatt ggatggcttg atcgttcaca
    81601 tacaattcag aaaatttaag tccaacttgt tcataaagtt tcgctaattt atgcttatct
    81661 tgggtagtgg aaatctcttg tttcaattgt gtaatctctg attgaatctt ttcggtcgct
    81721 tctggcgata gggctttttc ttcttttgcc agaacttttt tcggttcttt ttcttttttc
    81781 ttaaaaaatc caaacatcat tttccctact ttcaattaaa atgctttcaa aactgggctt
    81841 aagatccagg catataagag tgctgcggct actgtatcta catcggttgc tgtagcattg
    81901 ttgaatccca tcgaattaaa gatcgttact agcaaagcgg gcaataatgt aataaagaaa
    81961 ccatgagcaa taccgccaat gatcgctcct cggcgtccgc caactgcgtt gccaaatatc
    82021 cctgctgtcc ctccggcaaa gaaattggtt agcataccag gtaaaatcat cgctaagccg
    82081 aacatgggca aagtaaacat ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa
    82141 atgacagcat ttgggctata aggaaagaaa actggacaat ctaaggcagg gatcgcatca
    82201 ggaactaatt tcatagctat ccctcgaaat gccggcacga tttcacctaa cagtagacgt
    82261 acaccagcta gcaatacata cacacccact acgaattgga tggcttgtaa aaaggcaaac
    82321 atgatatagt tttgatctcc tgataattca gaagcgaatg gttcgcctgc aaataaaaca
    82381 gtgatgatat atagcgggac catcacgacc atgacagaca aataagtatc ttgcagaaat
    82441 tcgaaatgtg tcggcaactt gatatcttcg atcgactttt tattttcgcc tttctcacca
    82501 aaaagcttag caacaccggc ttcaaataag tacccgatcg tacaaaaatg acctaaagca
    82561 atatcatttg atccagtgat tttacgaatg atcggttgcg ctactgccgg catagcaatg
    82621 gcgaaacaag ctccgataaa accaccgacc aagatcaata cgatccctcg cagaccggca
    82681 aaatagccaa agactgtcgt catcgttgcc atccataaaa ttgcttgacc agttaaaaaa
    82741 atgtacttcc attttgtgaa acgagccaaa ataatattga atacaaaaat ggctaagaac
    82801 gtcagtgcaa tatctctacc taaacccaaa tcattcattg cttgaccatt gatagattca
    82861 atagaaggga tgatcccttg catatgaaat ccttcagtaa agattttccc gaaataagtt
    82921 aaacttccaa cgatgatgct cgaaccggca ctcaacactt gaaatcctag gagtgtcttt
    82981 aacgtgccag agatgacttg tcctgctgat ttcttctgca gtagtagacc caacatagca
    83041 atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt cgatgataaa attgataacg
    83101 cccatgtt
//
LOCUS       DAZKOW010000004        53815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000004 DAZKOW010000000
VERSION     DAZKOW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.004"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..421
                     /locus_tag="ACG912_000272"
     CDS             185..421
                     /locus_tag="ACG912_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFQ0511625.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            440..655
                     /locus_tag="ACG912_000273"
     CDS             440..655
                     /locus_tag="ACG912_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFQ0511626.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1358..2110)
                     /locus_tag="ACG912_000274"
     CDS             complement(1358..2110)
                     /locus_tag="ACG912_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0511627.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2125..3372)
                     /locus_tag="ACG912_000275"
     CDS             complement(2125..3372)
                     /locus_tag="ACG912_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0511628.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(3448..3594)
                     /locus_tag="ACG912_000276"
     CDS             complement(3448..3594)
                     /locus_tag="ACG912_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HFQ0511629.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(3698..4009)
                     /locus_tag="ACG912_000277"
     CDS             complement(3698..4009)
                     /locus_tag="ACG912_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0511630.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(4011..4208)
                     /locus_tag="ACG912_000278"
     CDS             complement(4011..4208)
                     /locus_tag="ACG912_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0511631.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(4602..6329)
                     /locus_tag="ACG912_000279"
     CDS             complement(4602..6329)
                     /locus_tag="ACG912_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFQ0511632.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(6322..7515)
                     /locus_tag="ACG912_000280"
     CDS             complement(6322..7515)
                     /locus_tag="ACG912_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0511633.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(7702..8346)
                     /locus_tag="ACG912_000281"
     CDS             complement(7702..8346)
                     /locus_tag="ACG912_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0511634.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(8440..8574)
                     /locus_tag="ACG912_000282"
     CDS             complement(8440..8574)
                     /locus_tag="ACG912_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFQ0511635.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(8576..10708)
                     /gene="feoB"
                     /locus_tag="ACG912_000283"
     CDS             complement(8576..10708)
                     /gene="feoB"
                     /locus_tag="ACG912_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFQ0511636.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(10705..11178)
                     /locus_tag="ACG912_000284"
     CDS             complement(10705..11178)
                     /locus_tag="ACG912_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFQ0511637.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            11579..11803
                     /gene="nrdH"
                     /locus_tag="ACG912_000285"
     CDS             11579..11803
                     /gene="nrdH"
                     /locus_tag="ACG912_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFQ0511638.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            11962..14121
                     /gene="nrdE"
                     /locus_tag="ACG912_000286"
     CDS             11962..14121
                     /gene="nrdE"
                     /locus_tag="ACG912_000286"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFQ0511639.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            14171..15136
                     /gene="nrdF"
                     /locus_tag="ACG912_000287"
     CDS             14171..15136
                     /gene="nrdF"
                     /locus_tag="ACG912_000287"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFQ0511640.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(15225..16364)
                     /locus_tag="ACG912_000288"
     CDS             complement(15225..16364)
                     /locus_tag="ACG912_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0511641.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(16673..17386)
                     /locus_tag="ACG912_000289"
     CDS             complement(16673..17386)
                     /locus_tag="ACG912_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFQ0511642.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(17640..18341)
                     /gene="nagB"
                     /locus_tag="ACG912_000290"
     CDS             complement(17640..18341)
                     /gene="nagB"
                     /locus_tag="ACG912_000290"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFQ0511643.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            18575..20107
                     /locus_tag="ACG912_000291"
     CDS             18575..20107
                     /locus_tag="ACG912_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0511644.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(20298..21500)
                     /locus_tag="ACG912_000292"
     CDS             complement(20298..21500)
                     /locus_tag="ACG912_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0511645.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(21582..22271)
                     /locus_tag="ACG912_000293"
     CDS             complement(21582..22271)
                     /locus_tag="ACG912_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0511646.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(22377..22745)
                     /gene="rplL"
                     /locus_tag="ACG912_000294"
     CDS             complement(22377..22745)
                     /gene="rplL"
                     /locus_tag="ACG912_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFQ0511647.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(22798..23301)
                     /gene="rplJ"
                     /locus_tag="ACG912_000295"
     CDS             complement(22798..23301)
                     /gene="rplJ"
                     /locus_tag="ACG912_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFQ0511648.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(23505..23632)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(23711..24400)
                     /gene="rplA"
                     /locus_tag="ACG912_000296"
     CDS             complement(23711..24400)
                     /gene="rplA"
                     /locus_tag="ACG912_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFQ0511649.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(24504..24926)
                     /gene="rplK"
                     /locus_tag="ACG912_000297"
     CDS             complement(24504..24926)
                     /gene="rplK"
                     /locus_tag="ACG912_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFQ0511650.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            25269..25937
                     /gene="sdaAB"
                     /locus_tag="ACG912_000298"
     CDS             25269..25937
                     /gene="sdaAB"
                     /locus_tag="ACG912_000298"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFQ0511651.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            25950..26822
                     /gene="sdaAA"
                     /locus_tag="ACG912_000299"
     CDS             25950..26822
                     /gene="sdaAA"
                     /locus_tag="ACG912_000299"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFQ0511652.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(26913..28013)
                     /locus_tag="ACG912_000300"
                     /pseudo
     CDS             complement(26913..28013)
                     /locus_tag="ACG912_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(28105..28992)
                     /locus_tag="ACG912_000301"
     CDS             complement(28105..28992)
                     /locus_tag="ACG912_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0511653.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(29069..29614)
                     /gene="nusG"
                     /locus_tag="ACG912_000302"
     CDS             complement(29069..29614)
                     /gene="nusG"
                     /locus_tag="ACG912_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFQ0511654.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(29715..29885)
                     /gene="secE"
                     /locus_tag="ACG912_000303"
     CDS             complement(29715..29885)
                     /gene="secE"
                     /locus_tag="ACG912_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFQ0511655.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(29909..30061)
                     /gene="rpmG"
                     /locus_tag="ACG912_000304"
     CDS             complement(29909..30061)
                     /gene="rpmG"
                     /locus_tag="ACG912_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0511656.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            30223..30867
                     /gene="cbpA"
                     /locus_tag="ACG912_000305"
     CDS             30223..30867
                     /gene="cbpA"
                     /locus_tag="ACG912_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFQ0511657.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(30923..31426)
                     /locus_tag="ACG912_000306"
     CDS             complement(30923..31426)
                     /locus_tag="ACG912_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0511658.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(31439..31485)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            32023..32988
                     /locus_tag="ACG912_000307"
     CDS             32023..32988
                     /locus_tag="ACG912_000307"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0511659.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(33087..34259)
                     /locus_tag="ACG912_000308"
     CDS             complement(33087..34259)
                     /locus_tag="ACG912_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0511660.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(34246..35391)
                     /locus_tag="ACG912_000309"
     CDS             complement(34246..35391)
                     /locus_tag="ACG912_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0511661.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(35405..36329)
                     /locus_tag="ACG912_000310"
                     /pseudo
     CDS             complement(35405..36329)
                     /locus_tag="ACG912_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            36446..37525
                     /locus_tag="ACG912_000311"
     CDS             36446..37525
                     /locus_tag="ACG912_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0511662.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            37522..38150
                     /locus_tag="ACG912_000312"
                     /pseudo
     CDS             37522..38150
                     /locus_tag="ACG912_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(38260..38487)
                     /locus_tag="ACG912_000313"
     CDS             complement(38260..38487)
                     /locus_tag="ACG912_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0511663.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(38590..41178)
                     /locus_tag="ACG912_000314"
     CDS             complement(38590..41178)
                     /locus_tag="ACG912_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFQ0511664.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(41198..41857)
                     /locus_tag="ACG912_000315"
     CDS             complement(41198..41857)
                     /locus_tag="ACG912_000315"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0511665.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(41906..42409)
                     /gene="trmL"
                     /locus_tag="ACG912_000316"
     CDS             complement(41906..42409)
                     /gene="trmL"
                     /locus_tag="ACG912_000316"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFQ0511666.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(42630..43496)
                     /locus_tag="ACG912_000317"
     CDS             complement(42630..43496)
                     /locus_tag="ACG912_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0511667.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            43605..44246
                     /locus_tag="ACG912_000318"
     CDS             43605..44246
                     /locus_tag="ACG912_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFQ0511668.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(44308..45669)
                     /gene="mgtE"
                     /locus_tag="ACG912_000319"
     CDS             complement(44308..45669)
                     /gene="mgtE"
                     /locus_tag="ACG912_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFQ0511669.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(45687..46586)
                     /locus_tag="ACG912_000320"
     CDS             complement(45687..46586)
                     /locus_tag="ACG912_000320"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0511670.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(46588..47385)
                     /locus_tag="ACG912_000321"
     CDS             complement(46588..47385)
                     /locus_tag="ACG912_000321"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFQ0511671.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(47363..48067)
                     /locus_tag="ACG912_000322"
     CDS             complement(47363..48067)
                     /locus_tag="ACG912_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFQ0511672.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            48198..48774
                     /locus_tag="ACG912_000323"
                     /pseudo
     CDS             48198..48774
                     /locus_tag="ACG912_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            48892..49569
                     /locus_tag="ACG912_000324"
     CDS             48892..49569
                     /locus_tag="ACG912_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFQ0511673.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(49648..51456)
                     /gene="pepF"
                     /locus_tag="ACG912_000325"
     CDS             complement(49648..51456)
                     /gene="pepF"
                     /locus_tag="ACG912_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFQ0511674.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(51632..52630)
                     /locus_tag="ACG912_000326"
     CDS             complement(51632..52630)
                     /locus_tag="ACG912_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFQ0511675.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(52856..53509)
                     /locus_tag="ACG912_000327"
     CDS             complement(52856..53509)
                     /locus_tag="ACG912_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFQ0511676.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        16274 a        11093 c         9322 g        17126 t
ORIGIN      
        1 tgtctcataa ttccacaaaa actttgaaaa cagttcttga acactaactt tttcattcct
       61 gaatcaaatt tattttggaa attttactat tcaagaataa acttgataag tttcaggaag
      121 atttttactg ttaatcaaat acgtatgtta cagtagcacc gtaaaaaata ttatttaaag
      181 aagcgtgttt aaacatgcaa aatgtaaagg aactaagtat tgtggaaatg cacaaactgt
      241 tggtggaggc aataatgctt ggggaaaatt ggggcaagtt gtaggtggac ttaccacagg
      301 ggcagtaggt ggtgcaggtt taggaacagc aatttgcgga ccagcttgcg gtgttgttgg
      361 agggttatat ggagctgtcg ctggaggtgc tgcagctggc tgggatgctc gaaaaaaata
      421 aaaaaggaga ataagtagta tgcatattaa aaacacaaaa acaacattta tattatcttc
      481 agaagagttg aaaaatattc aaggtgggag tgccgtggga gttttaggca ctactttttc
      541 tggggcaaca gcaggagtaa aactttgttc agccggagga ccttatgcaa tagctgcttg
      601 tggtgtaggt ggagctttat taggtgctgg attcagcatg tggaccggtg cgtaaacaat
      661 taaaaaatat caaacctcaa gattggtttg tgtcgattac gaacataggt atgttcttta
      721 tcttttcaaa agtttggaat ggacagaact ttttactaaa gcttcttgtg ctagttttta
      781 taattatatt aaatttttat cttttattaa agtcagataa aaattctttg gaaatgtaat
      841 ggtagagata tttttacaaa aggacttgca gcgctgtagt tggtgctgca ggaggaccta
      901 tttcggccgt tacaggaggt attggtaatg tacttgtcgg atgtgcatta aagcctgttc
      961 ctgcaagtta aatttataaa aacaggaaag taattatctc aaatcctatc aagtacaatc
     1021 ataggattgc acttgacagg acaaattttt taactaaaaa atgaaaaaca ctttctaaaa
     1081 attagtagaa tttgccaaat ctttcgcatc ttccaccata catttaattt taattcccct
     1141 ttatggtact taataaaaac aagaaaagaa ctattcattt ggcccttttt tggttccaga
     1201 ccatagaaat attcctccag taactaatat tgctgttatg cacgtaacta ataacagcta
     1261 taaaaattgg tacaacttta ataggattca gaaaaatttg cactagtgaa ggaatctatt
     1321 ttattagaaa aatgcaatct ccctaaagaa aaaaccacta aacagtgctt tctttcaact
     1381 tctccttaat tttttgtgct ttccctaaag taaagtttct ggccaactct gaatcaaaat
     1441 atagtgaacg ttttttaaaa tctatttctt taacgttaaa gatatttact aaacacgctc
     1501 gactaattct tgttaacata ggatatttct tttcaagctc actaattctt ccataaaatt
     1561 cgtactgccc atctttagta cacaatgata atctatgtgg taattttgaa gcttctacaa
     1621 aataaatgtc atttatatca aaagtaaatg tttgagaacc tatggaaaaa acaaatgctt
     1681 gattcgtaac ctgttttgtt gcatctatcc tttcttgcgc taataacaat aactcaacaa
     1741 tctcgtttcg atactcatct agtgtttgat cttttgttac aaatcctaac gtttcaactc
     1801 ttctttttat tgttacgggt aacatctcat catgagtagt tgtaaaaatg atttttgctt
     1861 gaacatcata ttttctgatt acttctgcta attctatacc gttaacttca tgatttaaat
     1921 caatatctaa gaaatatatt ccttgctttg gtcggaattg tttgaggtat tttttaactt
     1981 ctaacggact ttgtgttttc aatactatct taaatacttt atcgtgaaaa agaatgaaat
     2041 tatcaataat tcgttctaac tgatttaatt gtataatttg atcttcacat aatattattg
     2101 gataactcat aaatcctcct tccctcaaaa caacaaaata acttgagtag taaattgata
     2161 cggatcgttt tgatagttca caaacatatt gggattcttt ttattgattt cctctatact
     2221 agataaacct aacccttgat gtcccttttt tgttgttatt ccttttttca ttagagtatc
     2281 tatagagagt agtatctctc tacaactatt cgatatcgaa atttctatct gtttatcatc
     2341 tcggtaaata gctagagaca gttctctatc ttgactctct atagatgctt caattgcatt
     2401 atcgagaata attccaagca ccctaataca gtcaaatatt ggaacaggaa cttcaataat
     2461 agtacttgga cattcgaatt tacaacaaat cttattgtta tttgcttggt atagcttcgc
     2521 tatcaacaaa cttttcaaat attcattttt tatatttttt aaataacgat attcaaactt
     2581 attggttaga taagaattag aatagatctc taattctttt atttcttttt ccaatatcga
     2641 atcatggttt tgattagcga tttcttttaa actcagcaat aaatttttat agtcatgtct
     2701 aaatctggca aatttttctt gatctttttc cagtgaatca gtatattttt ttagataaac
     2761 caattctttt cttttcaatt gctgttcata ttgttctcgt tgcctaatag ttattctaat
     2821 aaataaaaat agaacaacaa acatttgaat gattaaaaat attaagacac caaacacaaa
     2881 atgatcaaat acttgatatt gatgagcaac ataagaaaca aataaaccaa ttgtaagtaa
     2941 ataaatcatg aatatagcag taagtttaga actggactgt tcaacaatcc tatttatagc
     3001 taattttcta tataaccaaa taaacatcag caaaaacaaa gaatcaagca ttatctggaa
     3061 tactacataa ccataccctt ttatcccttc aaatgaaaac atatacgtca taaccatgga
     3121 agtaataata gatctgaaat catcaataag catacataat agtaatttat ttaaaagcac
     3181 tatattagtt ttcttctttt cctttaatat aaaataactt ccgattatag gtatagctgc
     3241 ggaaacatca gtaaataatt caacaaaaaa agtgagtggt atcaataaac atacaaaaag
     3301 atattttctt ttgttaagat cagtattagt catataaaat aagagaaaaa agaaaaagtt
     3361 tcccacgaac attcttaata gataagcttt tgtatcgata gtaatcaaga gatcccctcc
     3421 tgattttagg atatcataaa aaaaatttta gttacacttt ttaaataatg aaaaaacaca
     3481 ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc ctttaatttt
     3541 tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt ccattacgat
     3601 tccccctaca aactataaat acttttatgt cactgattca atatacctat ttttctaatc
     3661 aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat ttatctccat
     3721 aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc aaatttctta
     3781 ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta aaatatagat
     3841 aattcaccaa tctatttatt aaaggaccgg gatctaattc atatttttgt ttttctaatt
     3901 ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct ttacttatag
     3961 atatatcatt atataattca tgaacaattt gcttagcatt ttttttcatt ttagcacttc
     4021 cctggaattg ctccacctaa aaaaccacct atagacattc ctgcaataca agtagttgcc
     4081 ttggcccaat cgaccgtaca tttattttta gtgcaataca ctccatttcc ataatatttt
     4141 ccactatgag tggtaccccc atagataagt tgtgtctctt taatagacaa aatttttaaa
     4201 tgtttcataa tctaatcacc cctaaattaa tgtttttaga ctattattct aacattcatt
     4261 aaatttcaaa aaacaattcc ttcttgaaac gtagcaaaat aattcctgaa taagaattta
     4321 atcactttta agtatataaa ctatgtatat tctttaatat tttttgagaa tgcacttcgt
     4381 acataataaa ctttaatcac attagtcttt catacctacg atcgatctct ttgactcgca
     4441 cccataaatt cataatagtt tgtcggcgtc tttggacgtt tcgaagatga gctacgcatt
     4501 ctcttttttt atagacaaaa aagatagaga cgaacatgga gcttccaaat ttggatattt
     4561 gatccaattt tggaagctcc atttatttcg tgtctgtctt tttatttttt ccagctttcg
     4621 actaattctt ggttctcttt gatccagtct tttgctgctt gctgtggatc ttttccattg
     4681 ttgatttcca acataacttt ttccatatct ttttctgacc aatggaaatt gtctaatact
     4741 ttgtaagctt ctggctggtc ttctttcaat ccttttcgaa ccatcgtatg gatctgctct
     4801 tcgcttccca ttccgttttc aggatcttct aagtatttca gatcatattt ggcaaacatc
     4861 caatgaggtg tccatcctgt aactacaatt ggttcgtgtt ttttgatcgc ttgacctagt
     4921 gcaacggtca ttgctcctga agaagatgtc tctactttcc agtctttcaa gttgtcatat
     4981 ttttggatcg tgttttcagc agccgtaact acaccagctc ctggctcgat tcctatgatt
     5041 ttcttacctg cctgatcggt taactcatcg atcgaattta catccatgta ggctggcact
     5101 gctaaaccaa cttttgctcc tgtcaaattg gcacctaggt cttcgacttg atctttatat
     5161 tgtgcatatt gtgaaccatg agttttaggt agccaagctg aaaccatggc atctgactcg
     5221 ccatttgaaa cagatttcca catgattgaa ttatctaatg gcgtcatcgt tacatcatag
     5281 cccatttgtt tcaatacttc accgactaca tttgttgaag caacttctgt atcccattca
     5341 acataagata agttgatacg ttttgtttct ttggcagatg aaaaactccc tataccaatc
     5401 gcacctaaga tcactactac tacagcacca atgatcagtc cttgacgttt tttacttttc
     5461 tgtgttgttg ctcctgcagc ttttttattg tttggctggt tcaaatgctg tgtaaaacgg
     5521 tcaatgatga ttgctaagat aactaatgca accccgttga caaatccatt cccgacttgt
     5581 gctctttgca atgctgataa tacgccgcgt cctaagccgg gtgcaccgat catagaggca
     5641 ataacgacca ttgaaagtga cagcattgtt gtttgattga tccctgccat gatcgtactt
     5701 ttagctaaag gcaattctag tttgaatagt ttttgacgac ttgtgctccc gaatgaatca
     5761 gaagcttcta ctaattcttt aggaacttgt cgaatcccta aattcgtaaa acgaacggtt
     5821 ggtggcagag caaagatgac tgaggcaaat acccctggaa ccatcccgat accgaaaaat
     5881 gcaactgctg gaatcaaata aacgaatcct ggcattgtct gcataaagtc aaggatcggt
     5941 ttgataatac tttgggcttt actgcttttt gccatcaaga tccctaatgg taccccaatg
     6001 atgatcgaga tcacactaga taaaagaaca agtgtgacag tattcatcaa atcattccat
     6061 agaccttggt tataaataaa ccatagaccg attaatgtga acaacgtaag tcctggacgt
     6121 ttcttagaaa taaagaaagc tgctaccgtc aataacaaga taaacaaagg agctggaatc
     6181 actaacagta aatttgtaat ccccgacatc actgcctgtc caatcgtttg taaaaatgaa
     6241 aataggccag aaaaagttgt cgtaaaccat tctgtgatat tttctaccca gtttgccact
     6301 ggtaattgat agttcaatag attattcatg ttcactcacc tcgcttgttt ctgctaatgc
     6361 ttcaatcaca ctacctcgga tgacgacacc ttttaatttt ccatcctcta caactgcaag
     6421 tggagtcggt gagtcataaa taatattgaa aatatcactt accaccatgt ctttcccaat
     6481 caccgtcaca ttttcatcta ggaagtcgat caatggacgt ttttgcttcc tagcttctaa
     6541 cgcttgatct gcagtgatga tccctttcag gtgtctcttt ctgtcaacag ccatcagcat
     6601 gctgacttct tcattacgca tccttgtcaa tgcaacgttt ggtccatcag attcgatatt
     6661 cgtcgtcaat gcaggtacca tgatgttttg agccgtaagc actttggaac gatcgacttc
     6721 ttccacaaat tcacgtacat aatcatttgc tggattggtc aatatttctt cacctgttcc
     6781 gatctgcatg atttcgccgt ctttcatcaa ggcgattctg tccccaatcc gtaaagcttc
     6841 attcaaatca tgggtaatga aaataatcgt tttttgaaca ttggcttgca ggtccagtaa
     6901 ttcgtcttgc atttctcgcc ggatcagcgg atcaagagcc gagaacgctt cgtccatcag
     6961 caagatctca ggatcatttg ctaaagctct ggctaggcca acccgttgtt gcatcccacc
     7021 agataactga ctaggatatt ggtctttaaa tgaaagcaaa ctagaatttt ctaatgcttt
     7081 ttctgctttt tcttgtcgtt cttctttagg tacaccacgg acttctaacc cgtattctgt
     7141 attttccaag atcgttcgat gtggaaacag accgaagttt tggaaaacca tattgatttt
     7201 atgacgacgg acttctcgca attcttcttt gcttaacttc gatacatctt ctccatcaat
     7261 atagatactg cccgaagttg gctcaatcag acgattcaat agacggatca atgtggattt
     7321 cccactcccg gaaagcccca tgatcacgaa gatttctcct tcttttacgt caaaattcac
     7381 gtcatacaca ccaaccgttg caccggtttt ctttaaaatc tccgttttcg gctggtgatc
     7441 attcatcata tctaaagcgg cttgtgtctt tttaccaaat atctttgtta aatgttttac
     7501 ttctactttt accaaacaaa ttctcctttt ttatgtaaat tcatttttct ttctgaaaaa
     7561 agtgaccaca ctatgttaac atcatgtagt cactttgtca aaaatgaaag aaatttgctt
     7621 gtccaaactg aaaaaaactt ggctaaatct ttgtaaataa aaggtttagc caagttaata
     7681 tttttttaat atttcacaca atcattctgc aagctgatgc tccgggtaaa aaaagtttgt
     7741 catacgctgt agtgtatatt catttccaat aaaatgaacg atgtcccctg ccgtaaattt
     7801 agcatagggg ccaggtgaaa caagcagttc ttcattatga gaaattgcga taactgtggc
     7861 gccagtcgcc tgccagatat tcaactcgct aattgaacgg tccagatgca ttgctttttc
     7921 tgataaagat aattcaaacg gaagtaaagg attcatctta tgtattcgct tcgtttgatt
     7981 gaccagctga tccaataatt gagaaaaatg attcagctca tcttgttgct tctccacact
     8041 tgccatgatt tctgcttttg tatcttggat ggattgtaca tcttgatatt gcaataggaa
     8101 atcttttgct ttttccttcg aagcaacaaa tgccccactt ccatgccgta cttccatgat
     8161 tccaagatct accagcacat tgatagcctt gcgggtagtc tcaggagaag cattgtacgt
     8221 actggcaagt gttgagcggg catggatctt ctcacccact ttatatcggt tttcaacgat
     8281 acgttcggct aaatcaacag caatctgctg gtagcgcggg cggacgattt ttttctttcc
     8341 agacattttc tttcctcttt ctttttttac tttccctcat cttactatgc tctagcaaaa
     8401 taagcaaaaa aggggagaat agcctcttgc aacgactgat catcgcttga tctgttcctt
     8461 ttttactggg caagctgttt gacagtcatc acaattctta ccggatttta aacgagtata
     8521 gacaattgcg cctgcacttc caaagattaa cacacttaat aagaatgtcg ccatcctatt
     8581 cctctccttc taatgacaat atagtataaa actggttctt ggattttggc tgacggaaca
     8641 aaacataaac gagtcctatc atcagtaaaa tagcaagata cgtccctaac gctacctgtc
     8701 caccctccaa taagtgtccc aactgataaa tcacaaaact aactaaataa gctaaaccgc
     8761 attgatagcc tatggcgatc caagtccatt tcttggcatt catttcccga tggatagccc
     8821 caattgccgc aaaacaaggc gcacaaagta aattgaaggc taacaatgaa taagctgctg
     8881 cgggagtata tgtgtgttgt agcacttgcc agatttctct gccattttcg gatacttccg
     8941 catgaccaaa caaaatacca aatgtgccaa taatattttc tttagcgacc aatcctgtaa
     9001 tcgctgcgac tgttccttgc cagtttcccc aaccaagcgg ctgaaaaaca actgccagga
     9061 ttcgcccgaa aaaggctaag atactttggt cttcagaaac aggttgagca taaaaattat
     9121 aagaagagct gaaccagatc acgatactag aaacaaagat aatcgttcct gcttttttaa
     9181 caaaagactt gctgcggtca taggtttgtc ttaggaccgc tgaccattga ggaaaatgat
     9241 atgcgggcaa ttccatgata aaaggagctg tatctccagc aaacaaacgt gtttttttca
     9301 agctgattcc agagaaaata attgcagtca taccaagaaa ataggcagaa ggagctaccc
     9361 aagattgatg aggaaaaaag gcccctgcta ctaaggcaat aatcggcaac tttgcagaac
     9421 aaggcatgaa ggttgtgacc atgatcgtca aacgacgatc ttgctcgttt tcaatcgtgc
     9481 gactggccat gacgcctggc actccacatc ctgtagcaat cagcatagga atgaaggatt
     9541 ttcctgaaag accgaatttc ctgaaaagtc gatccatgac aaaagcgatc ctcgccatat
     9601 atccgcagtc ttccagaaag ctcaagcata aaaacaatat aatcaattgc ggtagaaatc
     9661 ctaacactgc tcccacgcct gcgatgatgc cgttcaagat cagctcctgc atccatcccg
     9721 ctacttgcca ttcagctaac aatctgccaa catgttctgg taaccatgta ccaaacaatg
     9781 tgtcattgat ccaatctgtc cccattgttc ctactgtttg aatagccaga taatagatca
     9841 gccacatcac aaatgcgaag atcggcaagg ctaaccaacg attcgttgca atctgatcga
     9901 ttttgtcact catcgataat tggaaagaat catttgacac aacacagagt gtacatagtt
     9961 gggtaataaa ttcataacgt tcattgacca gaatcgtctc actatcgtcg tccaatagtt
    10021 tctctgttaa ctggatcagt tgctcgatct ctttttgctg agaaactgct atctcgtaat
    10081 cctctcttac cttctgatct cgttcaaaat atttcattgc ataccagcgt ttcttgccgc
    10141 tttgttctcc tagtacatct tcaatttcat gtaaagctgc ttctaaacgt ggatcatatg
    10201 tacagggaac aggaatagaa ggccgctttt ttgcagactc ctgaatcttt tcgattagtt
    10261 tttcgattcc ttgctttttc aatacgctga ttggtaaaac tggtacacca agaccatagg
    10321 aaagcttttc tagattgatt ttttttcctt cttttttgat gacatccatc atattcagac
    10381 caatgatcat aggaatgtct aaatccaata actgcgtagt taaataaaga tttcgttcta
    10441 aattggtagc atcaataatg ttgatgatca gttcaggctg ttcctgaacc aaataatctc
    10501 ttgcaatcac ttcttctggt gtataaggag acaaagaata gatgccgggt aggtcctgaa
    10561 tatgaatgtt cggatttttt ttcaacgatc cttctttttt ttctactgtt acgcccggcc
    10621 aattccctac atattgattc gaaccagtca agagattgaa caagctagtt ttcccgctgt
    10681 ttggatttcc gactaacgcg atattcattc tttttcctcc ctttgtatca agattagttc
    10741 ggcctctgac tttcgtaacg tcagttcata ccctcgcact gtcagttcga ttggatctcc
    10801 cagaggggct agttttcgga taactatttt tacatttcgc gtcaatccca tatccattaa
    10861 ccgtcgcttc actgcgcctt gtccgtggat tgctacgacg atcccagtct ctcctgcttt
    10921 taattgatca agagaagtcc agctttcaga ggaagcagtc tttttattga caaagatttg
    10981 acctagtatt tcccgactta ctgccactcg atgacttttt accagtaaaa tggcattgtt
    11041 gcctgataaa ttgacgacga cgattttact tccggcaacg atcccgatat tttgcaaatg
    11101 gcgttttgtt ttttcttctg tttcgattcg ttcgacttga taaacgaaat tctgctttgc
    11161 ctgcgctaag gtctgcatat tgattcctct cttgtgaaat aaatcatgta ttaaaatcat
    11221 tcattatatg ttctaatttt cttacgtgtt acgcaaataa atgagaatca ttctcacata
    11281 gaatactctc ggtaattgag aatgtcaatt atacttgtga tattatgagc gaatctttag
    11341 tattgataga taaaaatgag aaaaaaatat ttcttcatag ttttccacaa ttttttctct
    11401 attaaatttg ctattttccc acacgatact tgttttcagc tcttttttca aaaacacaaa
    11461 acttagtatt ccacagtttc cacaacacaa tatgtagtgt ttgttgtttg acttttttac
    11521 taagagtttt atactatcta ttatagataa taagaatgtg ttagaggagg cactcaccat
    11581 gaacgtaaaa atcttttcta aaaataattg tatgcaatgt aagatggtca aacggttttt
    11641 aacagaaaat caaattgctt ttgaagaagt caatatcgat gaacagcctg aagcagtaga
    11701 ctggttgaaa gcacaaggat tccaaagtgt tcctgtcatt acttctgatg cagaaacagt
    11761 tatcggattc cgtcctgatc agttgcgtaa acttgccagc taacatgact aaaagaatca
    11821 aaacgtgata catggatttg aagggcttga gacagcacca gtttttgtcg caaacttcca
    11881 aatccttttt taaagcttaa aaacaatgca atgatttgca atcaagcatc atcaaatacg
    11941 aaatgaaggt tgggcgtacg tatgagtctt aaaaatttac aagatgtcag ttatttcaag
    12001 ctgaataatg aaatcaatcg gccagtcaat ggtcaaatcc ctttgaataa ggataaagaa
    12061 gctttagttg ccttttttga ggaaaatgtc aaaccaaata cgatgacttt tctatctatt
    12121 atggaaaaaa tcaattactt gatcgatcaa gaatatatag aagcagaatt cattcaattg
    12181 tattcaccat catttattga aaagctatat caatttttgg acgagcaaca tttttctttc
    12241 aaatctttta tggctgctta caaattttat tctcaatatg cgttgaaaac caacgacggt
    12301 tccgcttatc tggaaactta tgaagatcga gtagccttca atgcgcttta ctttgcgaac
    12361 ggggatgaag aattagcact tgctcttgca gacgaaatga tccatcaacg atatcagcca
    12421 gctacacctt ctttcttgaa tgctggacgt aaacgtcggg gcgaattggt gtcttgtttc
    12481 ttgactcaag tcactgacga tatgaactct atcgggcgct caatcaactc tgctttgcaa
    12541 ttgtcccgta ttggtggcgg cgtagggatt actttatcaa atcttcgtga agccggcgct
    12601 cctatcaaag gatatgaggg tgctgcaagt ggtgtcgttc cagtcatgaa attattcgaa
    12661 gatagtttca gctattctaa tcaattaggt cagcgtcaag gagctggcgt ggtttatttg
    12721 aacgttttcc atccagacat tattgctttc ctttcagcaa aaaaagaaaa tgcagatgaa
    12781 aagattcgcg taaaaactct ttctcttggc gtgatcgtac ctgataaatt ttatgaattg
    12841 acacgtaaaa acgaagatat gtatttattt agtccatata gtgtcgaaaa agaatatggt
    12901 gtcccattct cttatgtaaa tattacagct gagtatgatc atctagttgc aaatccaaac
    12961 attcgcaaac aaaaaatcaa agcgcgtgat cttgaaaatg aaatctcaaa attacaacaa
    13021 gaatctggct atccttacgt aatcaatatt gatacagcga accgcgagaa tccaatcgat
    13081 ggcaagatca tcatgagtaa tttatgttca gagattttac aagttcaaac gccttctgtg
    13141 attaacggca aacaggaata tgaagtatta ggaacggata tctcatgtaa cttaggctcg
    13201 acaaatatcg tgaatttgat gcaaagccct aactttggtc aatctgtgcg tacgatgact
    13261 cgtgcattga catttattac agatgcatct gatatcgatg tcgtaccacc gatccaaaac
    13321 ggaaataagc tgaatcatac gattggatta ggtgccatgg gcttacacac ttatcttgca
    13381 aaagaacaaa tggaatatgg ctcagaggat tcattagact ttacaaatat ttacttcatg
    13441 ctgttgaatt attggacact tgttgaaagc aacaatatcg ctcgtgaaag aaaacaagtt
    13501 ttccataatt ttgaaaaatc agcttatgct gacggcactt atttcgaaaa atacgtaact
    13561 ggagaattcc agccaaaatc agaaaaagtc aaagcattgt ttgaaggcat ttttatccca
    13621 actgctgaag attggaatgc tttgaaacaa gcagtaatga aagatggact gtatcatcaa
    13681 aatcgcttgg cagtagcacc aaatggctca atttcatata tcaacgatac gagtgcaagt
    13741 attcatccta tcactcgcat gatcgaagaa cgccaagaga agaaaatcgg taaaatttat
    13801 tacccagcgc cttatctatc caatgagacg attccgtatt acacttctgc ttatgatatg
    13861 gacatgcgta aagtaatcga tgtttatgca atggcacaac aacacgtcga tcaaggcatg
    13921 agtttgactt tattcatgcg ttctgaaatc ccagaaggca tttatgaatg gaagacaaca
    13981 acgaagcaaa cgacacgtga tttgaatatt ttaagacatt acgctttcca caaaggaatc
    14041 aaatccattt attacgtacg tacctttact gatgatgcag aagaaatcgg aagcaatcaa
    14101 tgtgaaagct gcgtgatcta atatcataga tagattcgct tattcatatt ttaattaaag
    14161 gagcattctc atgtcagaaa catattatga agcaataaac tggaatgaaa ttgaagatat
    14221 cattgataaa tccacttggg agaagctgac agagcaattt tggctagata cacgtatccc
    14281 actttcaaat gacctagatg attggcgtac attgtctcaa ttggaaaaag atacagtagg
    14341 acatgtattt ggcggtttga cactgctaga tactgtccaa tctgaaagtg gcatggatca
    14401 gttgcgtaaa gatgtccgca ctccccatga agaagccgtt ttgaacaata ttcaattcat
    14461 ggaatccgtt catgccaaaa gttattcttc tatttttagt acactgaata cgaaaaaaga
    14521 aatcgaagaa atttttgact ggacaaatac gaatccttat ctgcaaaaaa aagctgagcg
    14581 tatcaatgaa atctacaaac acggtacacc gctagaaaag aaaattgcca gcgtcttttt
    14641 agaaacattc ttgttctatt caggttttta tactcctctt tattatttag gaaacaacaa
    14701 attggctaac gtagcagaga tcatcaaact aatcatccga gatgaatctg tacatggaac
    14761 ctacatcggt tacaaattcc aattaggatt caacgagtta cctgaagcag aacaagaaac
    14821 gttaaaagac tggatgtaca atcttcttta tgagctttac gaaaacgaag aacgttatac
    14881 agaagaatta tacgatccaa tcggctggac agaagaagtc aagacattcc tacgctataa
    14941 tgccaacaaa gcattgatga acctagggat ggatccactc tttccagata ctgccaatga
    15001 tgtcaaccca atcgtaatga acgggatttc aacaggcaca agcaaccatg acttcttctc
    15061 acaagtcgga aacggctatt tgttaggtca tgtagaagcg atgaaagacg acgattattt
    15121 gattggattg gaataaggat ttattttaat tcaaaaattt ttcgaaaaat aaaaagagtt
    15181 tagcgtacag atattttaaa tgtacactga actctttttt atgcttattc acttgtttct
    15241 tccagcactt ttggactgtc taacttttcc gaaacatgtt ccattcttct caataaatag
    15301 attttccgga tatttaatat tatcgtctta atcactgcat agacaggtat gcaaatcagc
    15361 atgcctaata ttcctgcgac acttcctgct ccaatcaata ccaagatgat cgtcaaagga
    15421 tggatattca ttgatttccc aaataacaac ggcttgatca aatttccatc taattgttgg
    15481 atcactaaaa tcgacaacgc catatacaaa gcttgaaatg gagagataaa caaaccgaca
    15541 atgattgctg gtaccgctcc aataaaaggg ccgacatacg ggataatatt cgtgattccg
    15601 caaatgaccg caagcaacaa gctataaggc tgatcgaata ttctcattgc caaaaagctc
    15661 attacaccaa taatcaaagc atctaagatc gtactgctga tataggcaga taatgtttcg
    15721 tttaattctt ttaccgtttg gcgtaactct ttacgaatcg ctttcggaaa gaagtgagac
    15781 aacgcatcaa taaatttgtg cccatccttg aacataaata gcaaaataaa tggaacggta
    15841 aataacaaaa taaaaaattg catcataaat gaaatgattt ttgagacact tgcagataaa
    15901 ctgactaata tgatattgaa caaattacta agagataaat ttgctgatgc taattgttct
    15961 tccaaattga aatttttaaa ctcttcccgc tgcgccaatt catttaacca tttgcttgtt
    16021 tcatctgcat acattggcaa gctctgggtc aattgaattg tctgttccat taactgaggc
    16081 actacattca tgacagttaa aacaactaaa atgattacga ccaaaaacgt caataaaaaa
    16141 ccgaacactc tcgggatccg atgcttttcg agaaaaacaa cgattggatc gaacatataa
    16201 tagataaaac cagcaacaag caatggtaaa aaaactgccg aaagcatttt caatattgga
    16261 ctaaaaatat tcggcatttg taaaataaag tacacgccga tgatcgtgag tactagctcc
    16321 actgtccaga aaaataattt tgattgttta aaacgtgatt gcatacatct cctccaaggt
    16381 cctttgatac ttctattcta tcatttaaca aagagttacg cgctgagatt ttcatttgct
    16441 taaaaaaaac aaaaaaaata aacatatttt aatgagtaga cgagtactta aacaaaaaca
    16501 cggtttggag tagtgacaaa aattatactg cagtcccttt ttccaaataa acggtgttca
    16561 aaagcttgca tcttcgtaat aagcccagcc aaacgaaaaa tataaggaac tatttttatt
    16621 tggctgggct tattacttga tgtaggacat ctattttaca acctcagatc atttattgcg
    16681 caatactgac taaatgcaac tttgtgatat ttttgtgtac ggacaactcc tcgatcacat
    16741 cggcataagt gagccctttt ggaagatcaa tcgtatagat atttttgtac actcggtagt
    16801 catcaacgaa ttttacgtca aaattcacat cttcgattac gatatttttc tcttcaaaat
    16861 aatcggtaag aaaatctttg gtttcttttc tatggataaa ttggatttct aactttttag
    16921 tcgtaggtac atggatcacg cgtttcacta ctgtgagtgc aatagcaatc ccgataaaac
    16981 tgatgatcgc aatggaatag taacccatgc cgatggcgat ccctaatcca gcaattgccc
    17041 ataaagaagc ggcagtcgta agtcccgtca ccgattgttt cgtgacgatg atcgttcccg
    17101 cgcctaagaa accgacacca ctcaccactt gagcaatcag ccttgcttca tctgaacgaa
    17161 tcgttccagc aagctgtgga tactctaaag catctcttaa tgcaccacta gcgatctcaa
    17221 cttggatcaa tgcaatgata catgctccca tacaaactaa aatatgcgta cgcatacctg
    17281 ctggacgatt tttatattgt cgttcaaaac cgattgctcc gccaaatagc atagcgatcc
    17341 ctaaacgtaa aataatctct ggaattgtca ataccatatc attcatctaa acaccctcca
    17401 gtccaatata tcatgtctga aaggagcaga caaaaaaagt gcaattttta cctcttacga
    17461 aaaaaggact gtgacaaaat ttttgtcata gtccttttta tgaataaacg gtgttcaatc
    17521 agctgaccct cggtattact caacaaacgg caaaatatgg ggagctattt ttcctttgtc
    17581 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttagcctac tattgaaaat
    17641 tacaacttac ttgctgcttc ttcatcgatg atcacaacaa cattgtcgtg attttgcaat
    17701 acacttgctg gcaaatgatt cgttacaggt ccttcaatca tccctttgat tgcatcagct
    17761 ttgctttcgc caaatgccat caagacgatt tctttacttt tcataattga accgattccc
    17821 attgaaacag cttttgttgg tacatcttct gctttatcga agaatcgttt gtttgcattg
    17881 attgttgatt ctgttaagtc aactactgaa gtcgttccgt caaatgaagc accaggttca
    17941 ttgaaaccga tatgtccatt ttgcccgatt cctaagattt ggatatcgat tggatgtgca
    18001 tcgatgattt gatcatactt tttgcatgct gcttccagat cttctgcttt tccatctggt
    18061 acaaacgttt ctttgaatgg ttttttatca aataattgga catccatgaa atgacggtag
    18121 ctttgttcat ctgaaccgcc tagtccaacg tactcatcta agtttacaga agtcatttca
    18181 ctgaaatcca aatcgcttga agtcatttct ttatataaag taactggtgt gcttcctgtt
    18241 gccaatccta atacttttgc acctttgttc ataccttctt tgatgatttc aaatgctttt
    18301 tttccgcctt cttcagcgtt ttttacacga atgatttcca tagtgattgc tcctttatat
    18361 tttggtatag cccaattata gcattctttt tttcttttta caataattag tatagaccaa
    18421 attgcatttt tttcattttc ttgacaataa ataggaatcc gtgttaggtt tcataagatg
    18481 gttcaatcaa acaatccatg ttgcatgaaa accaagcaag tgattcactg tggacgcttt
    18541 atgtctgcag tttttttatg aaaaaggagt tattatgagt atactgacat taaacaacat
    18601 tacccagcaa tttggcgaca aaatcctcta tgaaggtgtc gatctgcaat taaatgctgg
    18661 cgaacatctt gggctgattg gacaaaacgg agccggtaaa agtacattga ttaaaatcat
    18721 cactggtgag atcttaccag atgacggaca gatccattgg caaaaaaata ttcacaaagg
    18781 ttacctcgat caatatgtgg aagtcgatga aacattaaca atcgaagaat ttctaaaaac
    18841 tgcgtacgca aaagaatacg aaaaggaaga aaaaattgct tttctttacc aaacatatag
    18901 cgagaccttc gatgattcct tgctggaaaa agctgggaaa ctccaaacgg aattagacca
    18961 aggagtgttt tatcaaatcg acaccttagt tgctgagatg agcagcgggt tggggatcga
    19021 tgtcttaggt ttagattcac tgttgaaaaa tctaagtggt ggacaacgtt caaaggtgat
    19081 tttagcaaaa ctacttttag aaaacccaga tgttctgatc ttagatgaac caaccaatca
    19141 cttagatgat cagcatatcc aatggctgac gcagtttctt caggattttg acggtgcgta
    19201 tatcgtgatt tcccatgacc aagaatttct tgatcagatc accacccata tcgcagacat
    19261 cgagtttgga aagattacta agtatactgg ccatctaaaa caagcattga aacaaaagga
    19321 acaaaatcgc gaaagctatt tgcgccaata tcatgctcaa caaaaacaaa tcgaaaagac
    19381 cgaagcttat attcgaaaat acaaagccgg ttcccgatca acgatggcaa aaagccgaca
    19441 aaaacaatta gataaaatcg aacggctgac gcctccagct tcagcaagta aagctgtctt
    19501 tgattttcca ttctcgccta ttgtcacgac attagcagtg gaaacaactg atttagtcat
    19561 cggatatgat cgaccgctac ttgcacccat caatttgacc atccgctttg gtgaaaatat
    19621 tgccattcga ggcttcaacg ggattggaaa atctacttta ttaaaaacac tgatcggtga
    19681 gattgaaaaa ataagtggcg atttccactt cccagataac acaaaaatca attacttttc
    19741 acaagatctg tactgggaaa atccattaga aacaccacta caatatttaa gcagtcaatt
    19801 tccaaaagcg actatcaaag aattacgtcg ccaactagct aaagcagggt tagttaatca
    19861 attagcaagt gaacctctca ctacattaag tggtggggaa caaactaagg tgaaacttgc
    19921 acagttgaca atgaaccaag gtaatttact gattctcgat gaaccgacga accatatcga
    19981 tcaagaaacg aaagaaagct tacaagacgg gatacagaaa tttccaggaa cagtaatcat
    20041 cgtctctcat gaacaagaat tttatcaaga tcttgtgcaa cgagtcattg agattgaacc
    20101 aaaataaacg tttgattgaa tagttgaaaa aaaggagctg tgacaaaagt tttgtcacaa
    20161 ctccttcttc tgaataaatg gtggccaatt agctgacctt cggtattagc tcaaaaaacg
    20221 gcaaaatatg aggagctatt tttatttggc tattcttatt acttgatgca gaacagcttt
    20281 tcacaacctc ttttcgtcta ttttttattt tcgagatttc tctttccttt aaaaacaact
    20341 gtttgaaaga agaacgcaca tccttgaaaa acaagcccgc taaccgttac acctaaccag
    20401 ccatactgct gccacattgt cgtccctagc aatgaaccag ccgcacctcc aataaagtac
    20461 ataaacataa atacagtatt atttcggttg cttgctgctt cacctagccc ctgtactcgt
    20521 gtttgattgg cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc
    20581 aaaatggcca aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca
    20641 agtcccatat acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt
    20701 gcagcaactg ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa
    20761 ttgtaggcgg gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca
    20821 aacatgaaaa atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc
    20881 ggcaaacttt taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc
    20941 ggcattttcc agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag
    21001 atcatccgcc aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc
    21061 cccacgagta aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct
    21121 agtaccgcac cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa
    21181 gaagcgcatg caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca
    21241 gataacgctg ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact
    21301 aaaaacagta accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca
    21361 cttgtgctaa cagataaact agctgctact tttgttccaa tcggctggat gtaatacata
    21421 ttggcaacaa caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt
    21481 tttttttcat tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga
    21541 aaagattgga aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt
    21601 ccttgttttt tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca
    21661 cttgcaacca acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac
    21721 gcatacaaca cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga
    21781 ggcaatttca tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata
    21841 tagcctaaca ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga
    21901 ttcgtttcgg ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg
    21961 ttaccaagag cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa
    22021 ccagcaatga ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc
    22081 aatacgatca agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct
    22141 gccttactcg ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac
    22201 cgatagccca ctaccagtat cagaatgatt cccaacgaga tcaatatcaa tcggattatt
    22261 ttctttttca tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa
    22321 aaacaaaaaa gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat
    22381 ttaacagtta cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct
    22441 ttagatacgc cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc
    22501 aagcctaaac cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct
    22561 gcagttaatt ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca
    22621 gctacagcta caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg
    22681 tcgttaagtt ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat
    22741 gccattataa tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta
    22801 agctgcatct ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac
    22861 tggcgcttgt agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc
    22921 caatgctgtg atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc
    22981 taatgctttt gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc
    23041 gttactgaat gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc
    23101 ttttttagct gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc
    23161 acgaagttgt ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac
    23221 tactactgat gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt
    23281 tgcgataatt gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt
    23341 ttgcactgaa aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac
    23401 ctattcactt cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt
    23461 tgctcaacca gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa
    23521 ctctatgcca ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag
    23581 gaaattaagg cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta
    23641 gcttagcatg ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc
    23701 taatatttaa ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga
    23761 tcgttacatt tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat
    23821 catggattgc tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg
    23881 gtacatggat atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt
    23941 ttactgcttt tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt
    24001 ttggtcctaa tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa
    24061 ctacatcaaa gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga
    24121 aatctgcacc agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag
    24181 tttgtgtttt accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt
    24241 ttttagggtc tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt
    24301 ttgtttcttt tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt
    24361 tttttaatgc atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt
    24421 ggttataacg gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa
    24481 gctgctgtct tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct
    24541 tcaaccatgc gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca
    24601 gcgatttctc ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct
    24661 gaaccttttt cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta
    24721 acgaatgtga atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct
    24781 tgatcagctg tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga
    24841 cctagtgcag gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta
    24901 acgatttttt ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa
    24961 ttggagatgc atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa
    25021 tgtgcaaaac aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt
    25081 ttctgctatt tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat
    25141 ttttcgttct catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa
    25201 gcaaacaata acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg
    25261 tggagaaaat gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga
    25321 ttggtccaag tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta
    25381 tatttggcga acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct
    25441 atcgggggca cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg
    25501 atgaacgttt agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg
    25561 ttcctaagag tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa
    25621 cacgaaagct ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat
    25681 tgaatggatt caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag
    25741 atgttccagg tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg
    25801 gcactatgac agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag
    25861 atgatcctca agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg
    25921 taaattattt cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc
    25981 aagcaaaagc ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg
    26041 gccgttcccg tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat
    26101 caatcaacga gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa
    26161 aaaaattaga tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag
    26221 ctgtacgcaa tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta
    26281 caccgactgc cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc
    26341 ttgatttgac acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag
    26401 tcatcgcaaa caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt
    26461 ctgcaagtgc aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag
    26521 cagcacaagc agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg
    26581 caggcttagt tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta
    26641 tctctgctga tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg
    26701 ctgcaatgta ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg
    26761 gcttagctgt tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt
    26821 gataaagctc cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag
    26881 agaggactaa ttgcccttct cttcgttttt tattaatcca tcaatttcgt atattttact
    26941 tcatcgatcg tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt
    27001 tcaaaaacag atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg
    27061 ccaatcgcaa ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg
    27121 acaccagagt cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat
    27181 acttcaaagg acgcaggacc tccatctagt tgacgtccac catgattgga tacccagata
    27241 ccatctgccc ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg
    27301 ataactggaa gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt
    27361 tttgcagctg cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt
    27421 tcgctatacg cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct
    27481 tcttcacggt aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct
    27541 tctactgctt tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg
    27601 aaaaactgcg gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa
    27661 gtgctgatgg aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct
    27721 ttttcatgtg ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca
    27781 attccgaaaa cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg
    27841 attttctttg tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca
    27901 ccgcgaatat agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag
    27961 gcaatgttta caatatcgat tggatgttct tcattacttg tatgataagt catagtagat
    28021 ccttctttca tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac
    28081 atttactctc agatactttt atcgctattc tttccaaata aatcgactta caagctgatc
    28141 tacttcttgg ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc
    28201 ctctatttca tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg
    28261 tactgttcca tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt
    28321 ggttatagga atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc
    28381 aggccccttt gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa
    28441 aggcgcgcca attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt
    28501 tcctaaatag cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc
    28561 tatgccgtag ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca
    28621 ctcgttattt ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga
    28681 caaatctcct gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac
    28741 tgcttgctga tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct
    28801 gtagccgtgg aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc
    28861 cgttttttct ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa
    28921 cgcaaacaaa ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat
    28981 tggttctgtc atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat
    29041 catatgatcg acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa
    29101 gttctgtact ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc
    29161 cgtcaacttc tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga
    29221 cttcgccaac ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga
    29281 tatgattgac ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa
    29341 cgaatcctgt cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca
    29401 tttcaacaag tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt
    29461 tgacttcagt ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca
    29521 tactttgcgc acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat
    29581 gcaagacata ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa
    29641 taaaaaaacc tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg
    29701 ttgtggctag cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt
    29761 acgtaaaaca tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg
    29821 agttgttttc tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat
    29881 ttcatgagac aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat
    29941 tacaatattt acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg
    30001 attttgtgta gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca
    30061 tttacttctg cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt
    30121 tttacttgtc aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt
    30181 aatttgaaat taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt
    30241 gtatataaga aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta
    30301 aaaattttag aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt
    30361 ttccgtggaa acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg
    30421 acgcttcctg tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct
    30481 ttcttcaaag tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa
    30541 aatcattttt atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg
    30601 agcgtagaac aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg
    30661 gctgctattg caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat
    30721 atcgaaaaag acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg
    30781 aaagtatgtg aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt
    30841 gaaaatttgc aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc
    30901 tttctttttt cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat
    30961 aatacatcaa tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat
    31021 aggttgtcca aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc
    31081 tgatggtaaa gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt
    31141 gcaataaagc tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca
    31201 tgataccaaa accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca
    31261 atttacggtt aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc
    31321 ctgaggatac aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat
    31381 ttacaacgat tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt
    31441 tctcctagtt tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata
    31501 attataacag acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc
    31561 ttaaacaatg taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg
    31621 tcataagaat tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa
    31681 acttaaataa ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta
    31741 gcaagcagct gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta
    31801 atggatcatg cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt
    31861 cctagacttc tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca
    31921 agtttctttc attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg
    31981 agtattaaaa tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat
    32041 atattgatca atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta
    32101 ccggtattaa aaaataagca tattgactat acacttcatt atagtgaata caaaggacac
    32161 gatgctgaga ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa
    32221 cagaaaacaa tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat
    32281 caggtacttg atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca
    32341 ggttctggag atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt
    32401 cagctgattt tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa
    32461 ataagtgaaa aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc
    32521 gtccatgcga caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca
    32581 ttttcgtatt tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg
    32641 gtagatgcag gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag
    32701 catccttttt ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc
    32761 attgattttg tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta
    32821 cttatacaga aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta
    32881 cgtatcgttt caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga
    32941 ccttttgata tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt
    33001 tgtcagaagc tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt
    33061 gtcacagtcc tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag
    33121 tttctttctt tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag
    33181 acacaaccat tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg
    33241 atcaagttat tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc
    33301 atttctggca agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata
    33361 aaaatatctt tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc
    33421 gaaaagctaa tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt
    33481 atatcccacg aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac
    33541 agcagcgagt aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg
    33601 ggcgcactct ggtttcgttt actgatctct ttgcgattaa atgctagatt gatgacgctg
    33661 aaaccgctaa aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc
    33721 aacagctttt tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag
    33781 agtgccattt gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga
    33841 atcagtgttt gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct
    33901 gggcgactct gtatcttgat cgtcggcatc tttcctactt cgacttgttc ggtaaagtct
    33961 gcaggcagat gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt
    34021 tgtgaggttc catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca
    34081 tcgactacag gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta
    34141 ttttcaacat caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct
    34201 atcagtaaat tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata
    34261 acgcacccct ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa
    34321 ccatagaaga ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact
    34381 ttcgctgatc aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca
    34441 atacttcact tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga
    34501 aatcaagatg ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt
    34561 acctaacaaa atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg
    34621 ttcaccaaag tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt
    34681 caaaacaatc gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg
    34741 aatcaaggac aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg
    34801 gaccccccac atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact
    34861 tttgaaactc atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac
    34921 agtcatgatg ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc
    34981 aatacgctgt aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg
    35041 acccacatgg agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc
    35101 atttagcttt tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc
    35161 attgacttcg acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc
    35221 tttgattaca attccagtat taatcgtttc gagattgcca ccttgatcaa tctcgccaaa
    35281 ggccgccata aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga
    35341 tttatttcgc agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc
    35401 tcctctagtc tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt
    35461 ctttggttgc tatttttccg taactgatct tttcttcttg taagattgta agaatcggca
    35521 acaatactgt atttagttca gtagttgtta cttggagctg acctttttta taagcgattc
    35581 catcagaagc tattgttcgc tgacaacacg ttttctggga gtaatgggat gtcgatcgtc
    35641 aaacgatccg ccgtccgtac catatctttt agagattctt tggttccttt agcaatcacc
    35701 tgtccttgat ccatgatcac gatctggtcg caaagaattt ctacttcttc catgtaatgt
    35761 gttgtataga caatggttgc tccttgtcga ttcagctcct tgatactttc taaaatttta
    35821 ttccgacttt gggggtctac agctactgtc ggctcatcta aaaaaattaa ccgcggcttg
    35881 tgggcaatcc cacatgcgat gttcaaccga cgtttcaagc caccactaag ttctcctgga
    35941 cgaaacgaaa catatttttc taatcccact aattgaatca cctcttcaat caaggctttc
    36001 cttttctctt tttcggaaac atataaaccg caaaaataat cgatattttc ttgcacactt
    36061 agttcgtcaa agaccgcaac gtcttgagga acgaccccaa tcattgcctt gacagcataa
    36121 ttttcaggag tcattgttgt cccgaacaat ttaatggttc ctttgtcata atttaacaaa
    36181 gaaagcatac agttgatggc agtcgatttc ccactgccgt ttggtcctaa cagacctaag
    36241 acttctcctt cttgtacttg cagatcaaaa tgattcaatg ctacaaaatc tccatagcgt
    36301 ttgactaatt tatcaatttc gataatcatc atgaaaaact ccttccaaat ttcttgttga
    36361 tctgactata ataaagtgca agaaaagaaa ctagtgacga ttggctaaac cacacatgac
    36421 atttgtcaca atgaaggagt ctgctatgca taaatctcgg ctactcaacg aaactagctt
    36481 tctactggtt atctcattgc ttttgctggt aagtcaagga ttcgttgcta tgcaagttat
    36541 ctatttattg atcgccgttt tattcactgg tgtcagttat ttactgccta caaagccaaa
    36601 ttggattttc caagttattc tatttctttt atgtgccttt ttacctgatc ttgtcctgtt
    36661 tctgccagcg acgatgcgag tgatcgcccc tgaagaaagc caccttgatt acttgttttt
    36721 gttaggtttt ttgttgattt cgacggttgg taatcatccc cccatcaagg tcgcctttgt
    36781 cgtaattttg ctgttttttt ccttttactt acgaaaaaaa gatcaacgaa cgaccgatct
    36841 tgagaaaaag tacattgtct ccaaagatac tcattgggaa aaacaacaag agttatcgga
    36901 acaaaataaa gcattggccc tgtcacaaga aacacaatta gccttgcaaa ttgtggaaga
    36961 acggaaccga attgcccgag acatccatga caatgtcggt catctgctgt caagtgcgat
    37021 tttacaaatt ggtgcgcttg aaatgatcaa tcaagaacca aaactaaaag aaccattaga
    37081 aaatttaagt gccaccgttc acactgggat ggatcggatt cgtcaaagtg tccatgacct
    37141 ccatgagaca tcgatctctt ttcaacactc cttagagttt ctgatcgctg attttccttc
    37201 ttctgttacg attgaaggaa acttgtttga aggattgaat gaaacgcagc aaaactgctt
    37261 cttaatggtc atcaaagaag ccttaaccaa cagtatcaaa catagtcatg ccaaaaccgt
    37321 tgttttacat tttcgaacgt tgccggcctt ttatcgcttg caaatcacga atgatggcac
    37381 cagcccacaa aaaaattctg ctcccggtat tggtttgaaa acgatgcgtc aacgtgttca
    37441 agaacaaaag ggacaagtcc atattttcca agaaaaccag caatttcagt taacgatcat
    37501 tttaccaagg gagggacacc aatgataaca gtagtcatta ttgagatgat ccatttgtga
    37561 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    37621 attgtgccaa ggatgcgcta gtcttatacg aaacccatcg tcccgatgtt ttgctgacgg
    37681 acatccgcat gcctaaacaa acgggcatcg atgctgccaa agaaattttg gctaaatttc
    37741 ccaaagccat ccttttgctt ttgacgacat tcaaagatga agaatacatc cgcgaagcat
    37801 tttccatcgg tgtcaaaggg tatttaatca aacagaactt gcaagcgatt attccttctg
    37861 tcaaatccgc ttataatgga caggccgtat ttggtaatga aatcatcgag actttcactc
    37921 aaatgatgaa gaataaaccg acgattgata gtcatgctta tgcttctttt agtgaacgtg
    37981 agcttgcaat catcaaagaa gtagctgccg ggaaaaacaa taaagaaatt gcagatgcct
    38041 tgtacttgag tgacggcact gttcgcaatt acatcagcca attactagaa aagctagatt
    38101 tacgggatcg cacgcaattg gctatctatt tttatcagca tttgcaataa gtcttatcta
    38161 acacaaaaaa acctgaatga tgctccttcg catcattcag gtttttttca catcttctat
    38221 tcaattgtcc cgcaaaccat ttcctcacga ttgaaagaat caaggattcg cagggctgcc
    38281 aggattcgtt ggattgttcg gcgtgttagg cggaaaaata ggcgccttgt ctaacgtttt
    38341 ttctagtttt gattcttcac gatcgtctcg ttctacatca ggactttcat tcaatttgaa
    38401 ttctttttcc atctcatcta actttttaaa gtcattgcca gattcttttg ctaatttcgc
    38461 taatttatct tcggtttctt tagccataaa gtaacgcccc ccttttgatt tcagcttagc
    38521 atatcacact gaaaaaacaa aagaaaacgc taaccaacga tcatttttag ctatttctca
    38581 tctttaaaat caagcatttt ttgccggtat aaaatcgtag ggagaactgt tttccatacc
    38641 aatcatcgga tctactaact ggttatcgat cacttctgct gtaagaacga aatctttaga
    38701 aacagcaggt tctacctctt ctttcttttc ttctccaaca aatgaagaaa acagatctgg
    38761 ttccactgtt tccattactt ggtttttcgt ttcattcgtc ccactgtcat cttctacagg
    38821 tagcttttct tttacaaccc gtggctcttt tgtatagttc ccatcttcaa atggatcatt
    38881 tccgaccaaa gaatcttcat aagctgctag tttggtccgg agagtgatat tcattttatc
    38941 attatccatg atccttgatt tcaacgtagt catccggctt gctgattcat tcgctacaca
    39001 agtgacatag gcttgctctt cccctaacaa aatcaacagt tccttactgc gagtcactgc
    39061 cgtgtagagc aaattacgct gaagcatccg ctgatattga tggaccatgg gcaaaataac
    39121 catcttgaat tcactgcctt gtgatttatg gattgagcaa caataagaaa gagtgatttt
    39181 attccattcg tttcttttat aagtgacctc atttgcatca aactggataa ctaattcatc
    39241 cactttatct tcagaatctt ttgccaagat gatacccgtg atttctccca tatctccatt
    39301 gaatacattc aattcgggag tattgactaa ttggaggact ttgtctccta tccgataaac
    39361 ggtgtcgttc caatgtactt ccttttttcc aggtttccct ggattgaata tctcctgcat
    39421 cattttattt aacgcatcga tccctgcagg tccccgatac attggtgcta atacttggat
    39481 atcttgtgca cggaatccct tatttttcgc tttttccaca attttagaga tcaagggttc
    39541 aatttgataa gcatcacttc ggaaatagga acgatctttt tgattctttg tgaaatcttt
    39601 tggcagacgt ccttctttga tctcatgggc aagaggaata atactagaac catcaccttg
    39661 ccgataaatc tgattgagtt ccattttggg aatttgttcg atttccaaaa gatcatggag
    39721 tacttgacca ggaccgacag agggaagctg atctttgtct cctactaaga taacttgcat
    39781 attcgtcgga atcgctttga ataatgtgtt agccagccat gtatcgacca tagacatctc
    39841 atcaacaatc aaaagtccgc cttctaattc tttcgtactg actgaaggtg ttttttcccg
    39901 tccattcagc cccagaagac gatggatcgt actggcaggc agacctgtcg tttcgttcat
    39961 tcgtttagct gctcggccag ttggtgctgc aagtagaata ggaaaaattt cttgggtata
    40021 ttttgaagga tccaattcca gtccgttcaa ctctgcaaaa agattgacga ttccgttgat
    40081 cacagtagtt ttccccgtac ctggtcctcc tgttaaaata aatagagggg aacggattgc
    40141 ttcagcgata gcttgttctt gcgaaggtcc atactgtatg ccgaatattt tttcgatacg
    40201 ccgaatattc ttctgcactt ctttttcagg ataatggatt tcttttttac gactgagaag
    40261 gcgctggatc gaattaccga tcccccattc agaaaagtac aaactgtttt cgtaaagatt
    40321 agtctcttct tgttgtatct ttccttcttc aacaagttgg ataattacag tagcaacctg
    40381 atcaggctgg atttcaattg ggcgacttgt ttctaaaata cggatcgttt gctgaagcaa
    40441 tgtctccgcc ggtacgtatg tatctcctgt ttgaactgca tgctgaaaaa tctgatgcaa
    40501 gatagctgct cgaattcttc tcggtgaatc tgctgcaata ccgatttgtt cggctagatt
    40561 atctgcacgt ttgaacccga ttccttctat gtcttcgact agctggtacg gattttcttc
    40621 gatgatatcc aaagactcgt tcttataggc ctggtagata ctgaaagcta actgactacc
    40681 gaacccgtaa cgattcagtc ccatgatcac ttgatccatc ccgtggttta gccgaatcgt
    40741 ctcagcaagc atttctcttt ttggcttcgt caaaccggag atttgtttta ataccgaagg
    40801 atcagcaatg attttttcga tagcatcttc tcccagcaat tctacaattt tttctgctgt
    40861 tttcttcccg attcctggga atttttcgct ggaaagatac gctacgatcc catttgcagt
    40921 tgttggcttt tcttgctcat atctttcgag tttaagctgc cttccgtatt taggatgatc
    40981 cactaattct ccataaaaac gataagtctc attttcttga atatcgccga aacttccggt
    41041 gactacaatc tctttttcac ggtaatcagc tgtggtttca ctgatgtgta ccaaaagcac
    41101 tttgtaaaaa ttactaggat tttgaaaaaa aatcgcggca actgtaccaa cgaaataagg
    41161 catttcttct ggctgcatcg gaagccgcct ccttcttcta tagtaaaggt tctggtgctg
    41221 tttcagttcc taaaaaatct gtttggttcc atactttcag ttcatatggc aataatctgc
    41281 aaggctcttc agtctctaaa atcgtcaaac tactattgaa caatccaccc atttcacgca
    41341 actcacttag ctcttttcct gtcatccatt gaatggctgc agttaaagaa gctccatgtc
    41401 ctacgaataa aagtgggcca tctttatgtt gggcaaccgc atctgctaca acagtttcga
    41461 tacgtgtgat tgccgattca atcgtttctc cgtgaaaagg agaaggatca tacagatcta
    41521 aatgattacg caaattcggc agattatccg gatatttctg gtacatttca tcgatcgact
    41581 gaccttctaa agtacccagc cctagttctc ttagcttatc tgtataaaaa atctctaccg
    41641 gacgagcaag ctctttgttg ataccttctg ctgtatcacg tgctcgcttt gctgtactgg
    41701 aataaattgc tttaaaagga acatatttta cctttttccc aaatgctttg atttcttcgt
    41761 aactttgtgg caaaagcgga gaatcgccat tgctcccttg aaaacgtctt tcctgattcc
    41821 attgtgtctt cccatgtcgg gtaaaatata gttccatacg cagcaaactc ctgtctttct
    41881 tttattttac tctataataa actgtctaat ccagcttgtc attttcatag tgatgcgtca
    41941 attctaaatt agggaatcct tgctggcgaa gtgcttcata cactatcatt gcagctgtat
    42001 tcgataaatt caatgagcgg acatgctcat cattcatagg gatgcgtaaa cacttttctt
    42061 cgttttcacg cataaatgtt tctggcaaac cggttgtttc ttttccaaat aaaaagaagt
    42121 gctcttctcc atcagcaaaa ttttcgtcac tatatacttt atgggcaaat tttgagatca
    42181 aatgtagttt acgattacct aaaaatgtta aaaaagcttc taaattactg tggtaagtaa
    42241 tatttacatc attccaataa tccaaccctg cccgttttag atgtttgtca tcagttgaaa
    42301 aacctagtgg ctcgattaaa tgaagcggag tattcgtggc tgcacacgta cgggcaatat
    42361 ttccagtgtt tgcagggatt aatggttcaa ataaaacgat atgattcatc aggatcattc
    42421 ctctcatatt tatgttcgtt tctattttct gacaatttga tttttgcata aaaaaaaacg
    42481 tatcaactcg gtgtcagtca ctgcgagtcg ttacgttagt gaagctcttc tcactaactt
    42541 ttatcagaac aggaacctga taaaaaccaa tgaaaaaaca acttgttgct aatataacac
    42601 cctcaacttt tttttgcaac ctctactcat taaaaactgc tagaaaaacg ttttctctta
    42661 gttcctttta ataattctat gtcaattgta atcttcttca aaggattctt atataagtct
    42721 gctttgattc gctcttctac ctgccattct aaaggtgaca tggccaaaag atctttttga
    42781 ttttcctctg gtatctcaaa ttcataggtt acacgctgac gttcagttaa tttcattgct
    42841 tcagcaaact tggctactac acgttcattg gaataactct gtttttcagg ttgattcgga
    42901 taaaaagcct gacgtagttc ctttaaataa ttttcttgtg gcaccacctt caacacttct
    42961 ccatcttctg agagtacacg ttggaattct tggtaatgag aaggcgaaaa gatatttaaa
    43021 accgcgttga atgtttgatc agcaaacggc aaattggtca aatcagctac acaccaaaat
    43081 gcttcggcag attgttctgt agctgcgtag actccttctt ttgaaatatc aaatccaaca
    43141 gcattttgaa tctttttcaa ttttgaaagt tcattcaaga agctaccttc tccgcagcca
    43201 acatctaata gcgagacttc ttcaggtaaa tgctgcgcaa tagtctcaat aacgggctga
    43261 tacatgccag attggatcat tctacgcctt ggaacgaaca ttccttgatc gtattctgtt
    43321 ttaaccgcat gggataaaaa ataaagtgtt ccttttttgg aaaggtcaaa acggtgatta
    43381 tttttgcata tcaagccatt atcagacttt tttacttctt cttggcaaac cggacagcgg
    43441 aacagctgat gattttctgc caaaaactgg cgtcctcggt caattttttt taacatagtt
    43501 ccccctcatt tctgttttat cttaccgtta gatgctcact ttggcaactg ctaaaaatat
    43561 gatacaatgc ctagaagaaa gtgaaagata aaggagaaga ttagatgatc aaagaatttt
    43621 gcgctgaaaa ctacacatct attcctctag caattgctag aggagccgat cggatcgaat
    43681 tgtgtgacaa cttagcggtc ggtggcacaa ccccgagtac tggtgtgatc gaagaaacgt
    43741 taagttatgc tggggaaaaa gatatccccg taatgacgat tattcgtcct agagggggcg
    43801 acttcgtcta taacgatatc gaattgaaaa tcatgcatac tgatttgatt gaagcaaaga
    43861 aattagggac ggatggcgta gtatttggtt gtcttactcc ttcaggctgg ctagacgaag
    43921 aggccttaga actattgatc gaaacagcag aagggttgca gatcaccttc catatggctt
    43981 ttgacagtat tcctgaagat cggcaattcg aagcaatcga ttggctggta gaacatggtg
    44041 ttgaccgtat cttgactcat ggcggtcctg ctgacaaaac tattgtagaa aatttcccac
    44101 ggcttaaaga attgattgcc tatacagatg gccgcttaat cattttgcct ggtggtggtg
    44161 tcactgcaga gaatgcagca catgtagcta aagaattgaa cgtaagcgaa gtacacggaa
    44221 ccaaaatcgt ggatcttcag ttataatctt ataaacaaaa aaacatttgc aacaggcata
    44281 atgcttattg caaatgtttt tgttttttta cataaaatag cccataaata aagaagcaat
    44341 attgaaataa atcagtacac tcgtcaaatc gctcaaagtc gtgataaagg gaccgcttgc
    44401 tactgctgga tcaaatccta gacgatccat tagcatcggg attaaactac ctgcaagatt
    44461 cgccactgtg attgcaaata gcatagccat ccctatgaca aaccccaaga taaaattatg
    44521 tttccaaaaa cctacgatta gaaaaatcgt gatccccgta acagctcctg tgacaacgcc
    44581 ggtcaagact tcagccaaaa caagtcgaac aaatccatga tctttctcat cagaaatcgc
    44641 aagtcgacga acagccacag ctagcgactg ggttccagca ttaccggcag ttcctgtaat
    44701 cagcgaaatg aagactgcta aaatactcgc ttcacttacc aagtcttcat agtgactgat
    44761 taaagaagcg gtcgacatac ctaaaaacaa cagagtgatc agccaaggca aacgtcgaga
    44821 cgctgccttt actggatttt cattaatttc ttccacattg accccggcaa gtcctgaata
    44881 atcgctagca gcttcatcat caataacgtc gatgatatca tcgactgtga cgattcctaa
    44941 caaatgatca tcataatcgg taacaggaag agctaagaag tcataatctc taaatgtttg
    45001 tgccacatct tcttggtcgt cgcctacatg aactgaaagc actcgttcgc tcatcagatc
    45061 agaaatcatt gcgtcgtcat cattcacgat taggtcgcgc agagaaataa cccctactag
    45121 ctgaccttct tgattaacga catagatgta atagattgtt tcagctacat ctgcttcgtt
    45181 cttcagcacg tacatcgcag agcgaacagt ttgattggct acgatcgaaa caaattcagt
    45241 tgtcatgatc gcgccagccg tttcatcctc gtaatggaga agttccttga tttcactggc
    45301 atcgtctgcc gacatcaaac ttaaatattt agcaatttgc tttttatcca gcgtattaag
    45361 caagtcaaca gcattatctg tatacatctc agaaagcata tcagctgcat agctaggacg
    45421 catttcatct aggtattctt tcatatgttc gttgtcttct tcaatcacat cgaacatatc
    45481 cgctaattct ttaggcgaca gataggaata gatcagctgc ctgttctctt ctgtaagaga
    45541 ttggtagaat tgcccctgct catagatatg catctctaaa aaactgtttc tgaatgcctg
    45601 aatattattt tcccttaaat cttgagtcaa ctgggcaaat ctttcctcta attcttgatt
    45661 ttcatccaaa ccaatctcac ctcgccttat tcttcaaacc gtctcacaac tgcctccatg
    45721 tctgcggcta aaggctgctt gaactccagc aactcttctg taaatggatg gtaaaaacgc
    45781 agatcataac aatgaagggc ttgtcttttt attcccagat ccattcgccc gccatacata
    45841 tcatcaccga gcagggcaca gccaattgcg gaaaaatgaa ctcttatttg atgtgtcctt
    45901 cctgtatgca gctggatatc aactaacgcg atttctgtta gtcgcttctc tatccagtac
    45961 tcagtctgtg ccgaacgtcc ttcatcagag accattcgct tcaatagcga agaataatct
    46021 cgggcgatcg gcaaatcgat atgtccatgt gccgataaat ctgaaagcct tccactgact
    46081 aaggcctgat attttttcac aaacttcttt tcacgaagtt gcatatctaa ttttgcgtgc
    46141 gcaaatccat gctttgcaaa aagcatcaaa ccagatgtat cccgatctag tcgcgtcacc
    46201 acatggatta cttggttttc gtaaccttgt tttttataat aagcctttac acgattagcc
    46261 attgtcccat ttggatggta ttgggcagga atagacgaaa cgcctgctgg tttatttact
    46321 accagaacgt gttcatcttc atacacgata tcgatcggtg tttcatccaa aagtacggtt
    46381 tcatgttctc cttcagcagg aatcacaatg gttaccttat catccttagc tagagaaaac
    46441 aagacatttt ctacttgatc attaactttg atttgaccgc cttgaaattt tattttcgct
    46501 aacagaccct ttgagatccc tttttctttt aaaaaatatt ttacttgctg agaatgatct
    46561 ttctcataat cccatgtaaa ctgcatatca atcctcacta ataaaggcat ctttcacacg
    46621 atgccaaaaa tgcatatgtc gataagaagc aaaatggatc cgttcttctg ctattcgata
    46681 ataaattgag cggatatttg cttttgcgac attcaattga tcaaccgtta caagataatc
    46741 ttctgtattt tccaatttta tttctagcca ttcatcatgg gcaatcacga tcggagaacc
    46801 caaagtacga aacactcgat tattcagtga agcaatctct gccaactgaa aggcattgat
    46861 actaggatgg atcacagcac cgccgacact tttattatat gctgtcgaac cagtaggcgt
    46921 agaaatcgtc aatccatctc ctctaaaact ttcaaacagc tcgtttttga tgtatacatc
    46981 ggctaccatc gtacgattgg cccgtttaat cgttgattca ttcagtgcta aaaaatgctg
    47041 gtccggtgtt tcatccaaat aactgatccg aacatccagc aagggataac taacactttg
    47101 ttcccggttc gttcgtaagc tgtctactag ttcttccagc tcgtaatccc gccaatccgt
    47161 gtaaaaccct aaatgacctg tatgcacgcc caagaaacgc acatcattca gacaatgact
    47221 gtagtgatgg aacgccgaca ataatgtgcc gtctccgccg actgagatga ccaattctgg
    47281 ctcatgatca tctcgttgaa tccctgcttg ttctaaaagt gcgttcaaac gttctgtcac
    47341 ttctatagtc tttgcttctt tattatgaac gatcgctact ttcatcccct gcctcctctc
    47401 tttcgctttc tttcttcaaa agcgttcgat agtagacatc atctgatttt cctcttccat
    47461 gggagaataa ccgctgtgct tcttggattt cttcacggat actggacatc tcttcatcca
    47521 actgataggc agcttctgcc gcacgctgaa gtcgttcact catttcttct ggaaaaacgc
    47581 cttgatattt ataattcagc gaatgctcaa tcgttgccca gaaattcatc gcaagagtcc
    47641 gaatctggat ttccgccaaa attttcttct caccgctgat cagctgtaca ggatactcga
    47701 tcaccagatg ataagaacgg tagccacttg cttttttatt tgtgatgtaa tctcgttctt
    47761 ggagaacttt catgtctttt cttttacgga tgatctcaac aacttgatgg atatcttcta
    47821 caaactgaca catgatcctt aacccggcaa tatcagacat ttcttcttct aaacgtccca
    47881 ttggaatatg ccgtaattgt gattttgtta caatactatc gactggcttg actctcccag
    47941 tgacaaattc aatcgggaca tgtcgatttt gttcacggaa ctgtttgcgg atcccccgca
    48001 gtttcacttt caattctgat acagtttgtt catatggtgc taaaaatgct tcccaatctc
    48061 gttccatagc tgatacctct acttcattaa cattcattgt ttattctacc atatatgcaa
    48121 agttttttcg tactcttgat ttatgcattg accaaaaaaa gaaaattagg caaaatagac
    48181 aaaaaggagt cgatcaaatg acacaaatgc ttgaaatcga atataaatct atgctgacaa
    48241 agcaagagta cgagatattc atcgctcact atcaattaac agataaagat ttccgtgtac
    48301 agactaatat ttatttcgat accgttgatg aacagctgaa gaaacaaaat tgtggcctac
    48361 ggatccgttt cgttggtcat caagctgaat atacattaaa aacacctgct gaaaaagggc
    48421 gtctcgaaac aacggatacc tttataacag aagaagcaga agcatttatc ttcgaaaaac
    48481 gtctgcctcg ttcaggcgca gtttttcaaa aactttctga tttgaatatc gatcctgctt
    48541 cattaatcca aaccggaaag cttacaacaa aacgggctga attccctatt gaagaaggac
    48601 ttttagctat tgatgaaagc tggggagaca atctacacga ttttgaactg gaactagagg
    48661 taaggggatg catctgtggg aaaaattgct tttataaatt ttttaaaacg tttttcttta
    48721 ccttatcgtc ctgctaagaa caagatccaa cgtatgcttg aagctaaaaa ttagaaacct
    48781 ttcattttca caaggcactt gagttcattt ttttactttt ttctgttaag ttataggatg
    48841 tgaaagaatc tagtcactag ttgtatagaa tctattttgg gggagattca tatgatcgaa
    48901 atctatttat ttgttaatcc tttaggggaa tactgtttcg aaatggaaca acagttatta
    48961 caatttatcg aagaagagta cgggacacca tcaaaagaaa agatgcaatt tcgctttctt
    49021 cctttggtca atttgcagac aattggggat gtcatgcaac aaaaaggaat ttctcagaac
    49081 aatcttgagg aacgtaatca cttattctca atgacttatt ctgcagcatt ggactgcaaa
    49141 gcggcgcagt ttcaaggaaa gaaaaaaggc cgccgttttt tgatcaaatt gcaagaagca
    49201 gtcggctgta atgacatacc atattctcaa gaacttgctg aatccatatt tgaagaaatc
    49261 ggcggcgatt tgaatatgtt taaagaagat cgtcagtctg acttcgttaa agaagtattc
    49321 ttttctgatc aaaaaattgc tcgggaaatg ggcatcacaa aacacccttc tgcagtcgtt
    49381 tacaattatg cctgcgaccg tgattttggt gttttagtag aagtaaaagg tacaagtagc
    49441 ctttggagaa tcacagagct gtgtaaaaca acagataact catatcaagt ttttaatggt
    49501 gaacatttac atacaaatga ttctcagttc cattcaagaa ctagccattt acacttgctt
    49561 tctaattaaa aaaatacgtc gactccctta caagagagcc gacgtatttt tttcgtttct
    49621 attcgagaaa atcaaaaata gaaagcctta taaacgatcg atcaattctt ctaattcatt
    49681 tagacgttgt tcaaacatcg acattgcatc ttcaatatat gcagcctgtg tcatatcaac
    49741 tcctgctttt ttcattactt ccacaggata atcgctgtta cctgctttta ggtaagccaa
    49801 gtagttctct agtgcctctg gttcctggtt tagtattttc ttcgccagtg ctgaagcagc
    49861 agaaaagcca gtcgagtatt ggaaaacata gtaattataa taaaaatgcg gtatccgtga
    49921 ccattcgaat ttaatttctg ggtcttcttc gactgctgga ccataatatt ttgcattcaa
    49981 tttaccataa ctatcactta ggtattcact agtcaatggc acaccttttt catcttccgt
    50041 atgcatgaaa tgctcgaatt cagcgaattg tgtctggcgg aaaactgttc ctttaaaccc
    50101 atccaagtaa tggttgagta cataagcccg tacacgaggg tctttttctg tttccaataa
    50161 atactccgtt aggatatttt cattcgttgt cgaagcaatt tctgctaaaa agatggagta
    50221 gtcgccgtac acataaggct ggttcgaacg agtgaaataa ctatgaacac tatgtcccat
    50281 ttcgtggaca agcgtaaata gctgatccag cgtatcatgc caattcaata aaatatatgg
    50341 atttgtgtca tagcttcccg aagaataagc accgcttcgt ttccctttat tttcgacaac
    50401 atcgatccaa cgttcagaga atgctttttc tacgatggcc atgtattctt cacccattgg
    50461 tttcagtgct tctaaagctt tttcttttgc ttcttcgtac gtaaaggtaa ttggagcttc
    50521 ccctaagacc ggtgtataaa gatcatacat gtgcagtttt tccacttcta ataaacgttt
    50581 ccgtaattcc atgtatcgat gtaacaaagg caaatgcttg tttaccacgt ctaccaaagt
    50641 atcgtataca ctttcaggaa tatgattatt gctcaaagac gcttctctgg cagagctgta
    50701 attacggact ttcgctttaa aattatgtcc ttttatatgt gtgcctaaag tagaagcaaa
    50761 tgtatttcta aattgttcgt aaacactgta caatccctta aatgctgctt cgcgcaccct
    50821 tcgatccgtg ctttctagca actgaccata cacgccatga gataattgga ctatttcacc
    50881 attttctcct tcaatcgttg gaaaaactag atctgcatta ttcaaaacag caaatgtatc
    50941 acttgaagca tcaaagattt cacctgctcc agcaagaaga gattcctgct cagcagataa
    51001 gacatgggct cgattatcta ctatttgctg gatataatgg cgatagactt ccaattttgg
    51061 ttcttctttg aaatattgcc aaatctggtc atctgacaat tgcaatattt ctggttcaaa
    51121 ccaggaaacg gcttcactaa ctttcgaaaa cagactgctt gctcttgcat aaagcgcttg
    51181 gtaatctgta tttccagtgt cttgatcgtt tttaaggtgc gcatatacat aaatgacttc
    51241 agtttggcga taaaccctca atacgaattc aatcgcattt aaaaattgag aagcgccttg
    51301 atcaagtgtt cctttgtgtt tttcagattg ttttaactct tctgataatt ccaaatattt
    51361 ctcatcaaac tcttggtcgc ttgaaaagat cttggtcaag tcccaagtta aattttcagg
    51421 caattcttct cgttttggca actgctttac ttccatgaaa tccactcctt ttcttccctt
    51481 agctctatct atcaaaatag atcatagtct aacatccaca tgtcacagca tctaactttt
    51541 ctactcattc atgtgtctgc ttccatcaat tttagcataa ctaaatcttg ttttacatag
    51601 aaaaccgatt cctagcctat ttttatactt actatttttt caaagaagac aagtgtatgc
    51661 attcgttaaa aaactgctga tagaccatct cttcatcaat cataggaaaa agccattccc
    51721 ttttatgttc ttttatttgg caaagccatg agttgaaatc aggaactttc tggttttcca
    51781 aggcttcttc gtacaacatt cgataaagaa aaacctgttc ttgtaaataa aagaaaaacc
    51841 gacttttttg atagatccac gatgatagat gcaaaagatg tttatgacgc aagtagcatt
    51901 gttcttgtat ggatactgta ttcttttgct tgtgaataag ttggcgtgtc agccatttct
    51961 tcccatcttc actcactttt tctatttttt tcatttggtt cattttaatt tcgttactag
    52021 tgaatatttc tttaaggtcc ctactagaaa aagtccagca gctactgcaa aaggaaatat
    52081 gccctgatac agtctctgta atatgatgat acaaataaag cttttgtttc ttgagatcag
    52141 ctttccagca atgcactcct ctttttctat tatagtagca gcaacttttt tctatcaatg
    52201 agaattgcga ttgtccaaga aaagaattac caagtatcca ccatacttgg taactgttat
    52261 tcaaataatt ttgggttctt tcaataaatc tctggtgaga cagtctagaa cattggattt
    52321 ctattgctag attcgccaac aaaatatccg gcctttgccg caacctcggc aaataggctt
    52381 ccaaaaatac tggttctact gactgtccca gccaatccat gaagcattcc tttaactgta
    52441 agtgctcttt tgattctctt tcactaaaga ccgaacagtt atctgatttt tgatgggcaa
    52501 aatgagcaca ttttttcttt ccctttttca aaatcactct ttttcgacag ccacgacaat
    52561 aatatttctt tcctttttct gcatgtttag caaaacagca cttattatat tcattcattg
    52621 caagcagcat ttttctccct ctgaacttat ttacgtctaa gaatttattt acacgaaaaa
    52681 agagggcgtg aaaaagctat tctgcatcaa gtaataagaa cagctaaaca aaaatagttc
    52741 ccaatatttt tcgtttggtt gggcttgtta ccgcagatgc aagcttttga acacatttat
    52801 acgaaaaaaa gactgcgaca caatttttgt cacagtctct tactataatg ctgatttata
    52861 caaaataaaa tttcgttcgc tcgatagcat tacgcgccat cagacattgt ccatattcat
    52921 tcaatgtttc tggtgtacga tcgctctttt cagcaaactc caaaattcga gcaatctgat
    52981 tctctacatc cgcttggttg agattttcca tccagaagtg aacgactagg taatagcgct
    53041 cttcgaataa atacaaatca gtccaagcag actctagttc gacatctgtt gcaagttgga
    53101 tcacttgttc aaaatcttca aaagcaaaag ttgcttgtct ttcctgttct tctagggcgc
    53161 ttccagataa ttcttcctcc ggatcgctgc caataatctg ctgacgaaga aagtcactca
    53221 catcttctga atcaacagta gaatcttctg attgagaaat ttcttcatcc ggaagatttt
    53281 tactgataaa cagttctaat ccgtctcctt ttggcaaaac ttggaaagtg acagcttcgc
    53341 ttcctcggaa ctcctcttcg atatcaactt cttccaaaat actataaaag aaattttcga
    53401 tttctttgtg attgccaagt aaatccaaaa atgaaacgcc ccgttcctct aaatcttcat
    53461 gaccaattag tacacggatc gtattttcat taatatgttc catttccatt ttgctacacc
    53521 tcactttgcc acaatgttta atatagattc attgtagcga aatcagctta aatgtaaaga
    53581 aaaaaaatct cgctgttaag cgaatttaat aaaaccagcg gatttttcaa cctttcaagc
    53641 tatcttattt aactataaca tttttggcag acgaatacta tatagccgac ttttcagcta
    53701 attttccctc tctatttttc gtgcaatcat catcttctac taccttttta tacatctaat
    53761 caagaatact ttttgtaaaa acaaaaaaac ttggaaaaac tatctttgta atagt
//
LOCUS       DAZKOW010000005        51752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000005 DAZKOW010000000
VERSION     DAZKOW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.005"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(100..924)
                     /locus_tag="ACG912_000328"
     CDS             complement(100..924)
                     /locus_tag="ACG912_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFQ0511677.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(897..1577)
                     /locus_tag="ACG912_000329"
     CDS             complement(897..1577)
                     /locus_tag="ACG912_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0511678.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1600..2538)
                     /locus_tag="ACG912_000330"
     CDS             complement(1600..2538)
                     /locus_tag="ACG912_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0511679.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2783..3634)
                     /gene="ispE"
                     /locus_tag="ACG912_000331"
     CDS             complement(2783..3634)
                     /gene="ispE"
                     /locus_tag="ACG912_000331"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFQ0511680.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3788..6361)
                     /gene="mprF"
                     /locus_tag="ACG912_000332"
     CDS             complement(3788..6361)
                     /gene="mprF"
                     /locus_tag="ACG912_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFQ0511681.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6502..7308
                     /gene="proC"
                     /locus_tag="ACG912_000333"
     CDS             6502..7308
                     /gene="proC"
                     /locus_tag="ACG912_000333"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFQ0511682.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7387..8943)
                     /gene="rny"
                     /locus_tag="ACG912_000334"
     CDS             complement(7387..8943)
                     /gene="rny"
                     /locus_tag="ACG912_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFQ0511683.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9354..10403)
                     /gene="recA"
                     /locus_tag="ACG912_000335"
     CDS             complement(9354..10403)
                     /gene="recA"
                     /locus_tag="ACG912_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFQ0511684.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFANPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10512..11753)
                     /locus_tag="ACG912_000336"
     CDS             complement(10512..11753)
                     /locus_tag="ACG912_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFQ0511685.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11993..12571)
                     /gene="pgsA"
                     /locus_tag="ACG912_000337"
     CDS             complement(11993..12571)
                     /gene="pgsA"
                     /locus_tag="ACG912_000337"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFQ0511686.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12780..13703)
                     /locus_tag="ACG912_000338"
     CDS             complement(12780..13703)
                     /locus_tag="ACG912_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFQ0511687.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14442..15728)
                     /locus_tag="ACG912_000339"
     CDS             complement(14442..15728)
                     /locus_tag="ACG912_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0511688.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15721..16989)
                     /locus_tag="ACG912_000340"
     CDS             complement(15721..16989)
                     /locus_tag="ACG912_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFQ0511689.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17241..17702
                     /gene="mscL"
                     /locus_tag="ACG912_000341"
     CDS             17241..17702
                     /gene="mscL"
                     /locus_tag="ACG912_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFQ0511690.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17761..18207)
                     /locus_tag="ACG912_000342"
     CDS             complement(17761..18207)
                     /locus_tag="ACG912_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0511691.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18300..18545)
                     /locus_tag="ACG912_000343"
     CDS             complement(18300..18545)
                     /locus_tag="ACG912_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFQ0511692.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18660..19238)
                     /locus_tag="ACG912_000344"
     CDS             complement(18660..19238)
                     /locus_tag="ACG912_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0511693.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19315..19845)
                     /locus_tag="ACG912_000345"
     CDS             complement(19315..19845)
                     /locus_tag="ACG912_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFQ0511694.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19849..20709)
                     /gene="rlmB"
                     /locus_tag="ACG912_000346"
     CDS             complement(19849..20709)
                     /gene="rlmB"
                     /locus_tag="ACG912_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFQ0511695.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20702..21097)
                     /locus_tag="ACG912_000347"
     CDS             complement(20702..21097)
                     /locus_tag="ACG912_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFQ0511696.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(21094..22506)
                     /gene="cysS"
                     /locus_tag="ACG912_000348"
     CDS             complement(21094..22506)
                     /gene="cysS"
                     /locus_tag="ACG912_000348"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFQ0511697.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22503..23039)
                     /gene="epsC"
                     /locus_tag="ACG912_000349"
     CDS             complement(22503..23039)
                     /gene="epsC"
                     /locus_tag="ACG912_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFQ0511698.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23125..23364)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23361..24821)
                     /gene="gltX"
                     /locus_tag="ACG912_000350"
     CDS             complement(23361..24821)
                     /gene="gltX"
                     /locus_tag="ACG912_000350"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFQ0511699.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24991..26148)
                     /locus_tag="ACG912_000351"
     CDS             complement(24991..26148)
                     /locus_tag="ACG912_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFQ0511700.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26182..27552)
                     /gene="radA"
                     /locus_tag="ACG912_000352"
     CDS             complement(26182..27552)
                     /gene="radA"
                     /locus_tag="ACG912_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFQ0511701.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27615..28142)
                     /locus_tag="ACG912_000353"
     CDS             complement(27615..28142)
                     /locus_tag="ACG912_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFQ0511702.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28372..29178
                     /gene="proB"
                     /locus_tag="ACG912_000354"
     CDS             28372..29178
                     /gene="proB"
                     /locus_tag="ACG912_000354"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFQ0511703.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29175..30422
                     /locus_tag="ACG912_000355"
     CDS             29175..30422
                     /locus_tag="ACG912_000355"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFQ0511704.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30498..30842
                     /locus_tag="ACG912_000356"
     CDS             30498..30842
                     /locus_tag="ACG912_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511705.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30959..31285)
                     /locus_tag="ACG912_000357"
     CDS             complement(30959..31285)
                     /locus_tag="ACG912_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511706.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31900..33102)
                     /locus_tag="ACG912_000358"
     CDS             complement(31900..33102)
                     /locus_tag="ACG912_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0511707.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33219..33734
                     /locus_tag="ACG912_000359"
     CDS             33219..33734
                     /locus_tag="ACG912_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0511708.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33933..34379)
                     /locus_tag="ACG912_000360"
     CDS             complement(33933..34379)
                     /locus_tag="ACG912_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0511709.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34426..35904)
                     /gene="msr(C)"
                     /locus_tag="ACG912_000361"
     CDS             complement(34426..35904)
                     /gene="msr(C)"
                     /locus_tag="ACG912_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0511710.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36258..36887)
                     /locus_tag="ACG912_000362"
     CDS             complement(36258..36887)
                     /locus_tag="ACG912_000362"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0511711.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36871..37335)
                     /locus_tag="ACG912_000363"
     CDS             complement(36871..37335)
                     /locus_tag="ACG912_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0511712.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37822..39090)
                     /locus_tag="ACG912_000364"
     CDS             complement(37822..39090)
                     /locus_tag="ACG912_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0511713.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39321..40058)
                     /locus_tag="ACG912_000365"
     CDS             complement(39321..40058)
                     /locus_tag="ACG912_000365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0511714.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(40125..40694)
                     /locus_tag="ACG912_000366"
     CDS             complement(40125..40694)
                     /locus_tag="ACG912_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0511715.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40822..41694
                     /locus_tag="ACG912_000367"
     CDS             40822..41694
                     /locus_tag="ACG912_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0511716.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            42036..42908
                     /locus_tag="ACG912_000368"
     CDS             42036..42908
                     /locus_tag="ACG912_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0511717.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            42905..44863
                     /gene="nagE"
                     /locus_tag="ACG912_000369"
     CDS             42905..44863
                     /gene="nagE"
                     /locus_tag="ACG912_000369"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFQ0511718.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(45195..46046)
                     /locus_tag="ACG912_000370"
     CDS             complement(45195..46046)
                     /locus_tag="ACG912_000370"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0511719.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWVLKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            46169..46636
                     /locus_tag="ACG912_000371"
     CDS             46169..46636
                     /locus_tag="ACG912_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFQ0511720.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(46899..47381)
                     /locus_tag="ACG912_000372"
     CDS             complement(46899..47381)
                     /locus_tag="ACG912_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0511721.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(47481..48344)
                     /locus_tag="ACG912_000373"
     CDS             complement(47481..48344)
                     /locus_tag="ACG912_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0511722.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            48942..49817
                     /locus_tag="ACG912_000374"
     CDS             48942..49817
                     /locus_tag="ACG912_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFQ0511723.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(49962..50114)
                     /locus_tag="ACG912_000375"
                     /pseudo
     CDS             complement(49962..50114)
                     /locus_tag="ACG912_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(50382..51713)
                     /locus_tag="ACG912_000376"
     CDS             complement(50382..51713)
                     /locus_tag="ACG912_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0511724.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        14993 a        10973 c         9164 g        16622 t
ORIGIN      
        1 cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag aagcctataa
       61 aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt ttcttctgaa
      121 ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa agactaatgt
      181 gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg acagcatccc
      241 tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc ctaatcgcat
      301 accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg caatcgggat
      361 catcacagca atagccacac cagtaatcac attgaacatc atcgacatca aatgaacggg
      421 tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg gccgtttgaa
      481 taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga cttgctccca
      541 tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg aattccctcc
      601 gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa tggcaatgga
      661 gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag ctgctaatac
      721 aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta acgctacccc
      781 tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga tcactaaaaa
      841 cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg ctcttctcat
      901 gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg gatctgtctg
      961 tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga tttcccatgg
     1021 acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc atccatacct
     1081 gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg cgctaaactg
     1141 attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat atcccacatc
     1201 cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag acgcttgaac
     1261 cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact aggaaaacct
     1321 gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt tccttcttga
     1381 tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa cgatgctttc
     1441 accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc acccgcatct
     1501 acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt caaatcagat
     1561 atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac tttcttttaa
     1621 agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga tttgttcatt
     1681 agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat tcgacaatgt
     1741 ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt tgtttttctt
     1801 cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg gtgaaacgcc
     1861 cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag cagcatgctg
     1921 agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt gatccagtgc
     1981 ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt tatctggata
     2041 tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata ccggatcgag
     2101 ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt cttcatgttc
     2161 gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca tcaaggtctg
     2221 tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg aactattata
     2281 cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt cgaaatcatg
     2341 cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta cgatctcttt
     2401 tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt ttttgtctgc
     2461 ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta ccgtagctgt
     2521 caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat tttacgattt
     2581 attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc catcaatttc
     2641 tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct gctcatatct
     2701 agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc agtttttcat
     2761 cacctatttt acttcttata ttttattgca gggttcgtac taaataaact tcctcacaaa
     2821 aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac aaggcaaaaa
     2881 cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc atacgctctt
     2941 tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg tttcccattt
     3001 ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg tggatgtctt
     3061 gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag acactcattc
     3121 ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg atttcttccc
     3181 ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct gtaccgactt
     3241 ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat tggttgatcc
     3301 ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct actggaatat
     3361 tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt agcaataaag
     3421 cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt gtttctacaa
     3481 tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct acgctcgaca
     3541 tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc agtcctaaat
     3601 ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga aaagtctaag
     3661 tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc tagtggttga
     3721 ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa acaagttcta
     3781 gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt cctttttctt
     3841 ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct ttcctataag
     3901 caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa gcagactgga
     3961 aggaatagaa aaaattacca taattatata cgatatgcat gactttttct tgaaagaaag
     4021 aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct tccgagtagc
     4081 ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat tcctcagatt
     4141 ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc ggctgagcgg
     4201 tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta gataatacat
     4261 atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga agccattttt
     4321 gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac caagtaaaat
     4381 ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga gacaagtctt
     4441 ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa cccaattcat
     4501 gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag cctaaacaat
     4561 ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg ccaactggat
     4621 ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact tgatcttggt
     4681 cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg cttgtagctg
     4741 tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca ctgccaagct
     4801 tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat gagatgccca
     4861 aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa tgatttcctg
     4921 ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa acgataaaca
     4981 aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg aattttgttc
     5041 gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat accaatatca
     5101 actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt aaagcaatcg
     5161 taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg agtaataaaa
     5221 agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc aataatactt
     5281 gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc atcatgatcc
     5341 ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa aacagtcgcg
     5401 gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac aatagaagtc
     5461 ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa actactgcta
     5521 catcttgccg aacagacaat tgactcattc ctaagatcat caaaacgtca aaagtcccca
     5581 ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac ataggataga
     5641 tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat accgcaaggg
     5701 ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt ggataaaact
     5761 cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt gctaaaaaac
     5821 taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga ataaaaaaga
     5881 catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat agtgcgactt
     5941 ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa ctagcccgca
     6001 gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt gttacccacg
     6061 cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct aagactctga
     6121 tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc agaatcatga
     6181 gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc caactcatgc
     6241 cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg aaaaaaatca
     6301 gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt ttatttttca
     6361 cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat cttatttatt
     6421 ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga aagatatcct
     6481 aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa tatggcacaa
     6541 gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt gttcgtttcc
     6601 agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca tgcatgcaaa
     6661 agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa accaaaaatc
     6721 attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt catttctatt
     6781 gcttcaggaa aatcattaat agatttagaa acagcgcttg aagatcctca ggcctccatc
     6841 gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc gatttgcaaa
     6901 aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga agcaatcggc
     6961 agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc tggcagctca
     7021 cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact gaatggtttg
     7081 tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg tcaaaaattc
     7141 ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc tggcggtaca
     7201 actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat catcaaagga
     7261 atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaataatg atagcaatgt
     7321 tttttcgtgc cttacaaaaa aagaagagct gtggcttttg ccgcggctct tcttttgtat
     7381 tttttattat ttagcataat cagttgcacg agtttcccga ataacgatta ctttgatgtg
     7441 tccaggataa tccagttctt cttcgattcg tttacgaata tcacgtacta aacgaacagc
     7501 gtctaaatcc gagatttctt ctggcttgac cattacccga acttcgcgtc cagcttgaac
     7561 tgcgtaactt gattctacac cggcgaagct gttcgaaata ttttccagac ttcttaaacg
     7621 acggatgtag ttttctaatg attcacttcg cgcaccaggt cttgctgctg agagcgcatc
     7681 tgctgcagct acaaggacag agataacaga agtagcttcc acatctccat gatgagaagc
     7741 gatcgcgttg attactactg ggttttcttt gtacttggca gctaattccg caccgatctc
     7801 aacgtgagat ccttcaatct catggtcaag tgctttccca atatcatgga gcaacccggc
     7861 acgttttgct aattggatat cttcgccaag ctctgctgct aagataccag ctaattttgc
     7921 aacctcgacc gaatgcttca agacgttttg tccataacta gttctgaatc gcagacgtcc
     7981 gatgatcttg attagatctg gatgaagtgt atgagcaccg acttcaaacg ctgcctcttc
     8041 cccgtattcg cgaatacgtt catccatttc tttacgagat ttctcgacca tttcttcgat
     8101 acgcgctgga tggattcgtc catcttgtat cagtttttcc aaggtcatcc gtgcgatttc
     8161 tcttcggatt ggatcgaatc cggaaagaac aactgcttcc ggtgtatcat cgatgattaa
     8221 atcgatacca gtcagcgtct caagcgttcg aatattacga ccttctcgtc caatgatacg
     8281 acctttcatt tcatcattcg gaagggtaac gactgaaaca gtcgtttctg aaacttgatc
     8341 tgctgcgcag cgttgaatcg ctaatgataa taagtttttc gctttgcgat ctgcttcttc
     8401 tttggcacgc tgttcagatt cttttaccat tagagttaac tcatgattca gctcttcttc
     8461 agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat aaaccagcaa ttttttctag
     8521 ttctttttgt tgatcttcaa tcattttttc aacgtctttt tctcgctctt caattaattg
     8581 ctgttttgaa ctaagtgctt cttctttact ttcaagtgag ttctcgcgtt tttctaacga
     8641 gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa cgattctctt gagattttaa
     8701 ttctaactta ctttctctca gctcactttc aacttcagaa cggtatttct gattttcttc
     8761 cttagcttct aacaatgctt ctttttttag agtttctgct tctttttttg cactatcgat
     8821 gattccagca gctgatgatt gtgcaccagc tatttctttc tcgtgacgtg actttgcgac
     8881 cataaaccct aaaccaagac cgacaattaa accgatgata gcgaggagaa tgttgaatac
     8941 cataaaatcc acctccatac tatctttttc atttttatca tctatacagc ttctatgata
     9001 aattattcta aactcgtcaa tatgcatctt cgcatatatg tgtacaaaaa acatgcacaa
     9061 ctttattcta atgtttgtac cccgtagtgt caactttaaa taaacaaaga aacggctttc
     9121 tacttattca ttaaacgatg gtttttaaaa taaatgacac tagtttcata taggtaaacc
     9181 tttaaattga gaaacttttc tattaaatta ttctttgtag atgaacagat atttttattt
     9241 tatgtaccca ctcgtataat taaataactc acgatttctt tattcaataa aaaaacggca
     9301 ggtcaaggag agtacctact cttcagacca gccgtttttg tttatcataa tttttattct
     9361 tctactaaag gcaattcttc ttgtcctttt tcatcttctg ggacagcgac ttcttcacca
     9421 ataccataag ctgctctaac tagagcagaa acttctgcca tcatttcagg atgattagcc
     9481 atatagattt tagcgttttc ccgtccttga ccaatccgat cttctttata agaataccaa
     9541 gcaccgcttt tatcaacaat gtctttttct actgccatgt ctagaagttc tccttcttgt
     9601 gagatcccta aaccatacat gacatctact tcagcgattt tgaaaggagg tgctactttg
     9661 ttttttacga ctttgatctt tgtacggtta ccaacaatat ctgtaccttg tttcaattgt
     9721 tctgcacgac gtacttccaa acggatcgtt gcgtagaatt tcaatgcgcg tcctccagga
     9781 gtgatttctg gattcgcaaa catcacgcct actttttcac ggatttggtt gatgaaaata
     9841 gcgattgttt ttgttttgtt gatcgaacca gagagtttac gcaatgcttg agacatcaaa
     9901 cgtgcttgta acccgacatg tgagtcaccc atttcgccgt cgatttctgc tcgtggaact
     9961 aaagcagcaa ctgaatcgac tactacgata tctacagccc cacttgatac taaagcatca
    10021 gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa gaagtagttc atcgatattc
    10081 acacctaatt tttgtgcata ttgcggatct aacgcatgct cagcatcaat gaaagcggcc
    10141 gttccgccat ttttttgtac ttctgcaata gcgtgtagtg caactgttgt tttacctgaa
    10201 ctttcaggtc catatacttc gatgatacgt ccacgggggt aaccgccgac acctaaagcg
    10261 acatctaacg caagagagcc acttggaatt gtagagattt gttggtcgat tttttcgccc
    10321 aatttcataa tagagccttt accatagctc ttttcaattt ttttcagtgc agcatctaag
    10381 gctgctttac gatcatctgc caatacgata atcctcctta tatttacaaa tcagagcaac
    10441 atttcattat ccttatcata tcttttttag tgaaaaaaag caagaaaaaa ccgaacaaat
    10501 attcgcattt atcattttct taaaagctgt tttctcaaaa gatcaaatcc ttgcataacc
    10561 gcattatatc tgatagcagc acgatctctg gcaaagtgat atttttccga aaccacgccg
    10621 tctttagatg aaatagcgat ccatactgtt cctgcagatt gaccttctaa cgtgtccggt
    10681 ccagccactc cggtgaaagc tacactgtaa tctgttcttg ctatctcacg tgcttgaatg
    10741 gccatctgct ccacacattc tttgctgact gttccatatt ctgctaatag ctccggatca
    10801 atctccagaa aaccagcttt tgtttggagc gagtaggtaa cgaatccgcc tggaaatact
    10861 tcagatacac cagcaatgtc gcctaatgcg gcttggaagg cgccggctgt caaactttcc
    10921 gctgctgtta cagtctgctt gttttctttc agtagttcca ctacgacttt tgctagcgaa
    10981 ttatcatcgc cgtatccata aaaatattcc cctactcgtt cttggatttt ttcttctaat
    11041 gctaaaagag cttgattccc tgcctgaaca tcatttgttt tcactgtcag tcgtaatgtc
    11101 acttcatttg gtttggcata tggtgcgatc gttggattga tctgtgtctc aatcagatct
    11161 tttagctcag tgaccagtcg cgattcacca atcccataaa agcgcaaaac tttagaaatt
    11221 agtttttctt cagaaggaaa acgttcctct agtaaaggac gtacttgttc cacaaacatt
    11281 ggtttcaact cattaggcgg accgggaaga aggatatacg catgtgtatc tgtttgataa
    11341 aaagtcccta aagcaagccc tgtacgattt ggtagaggaa ccccgccttc gataatctgg
    11401 gactgttgca gattgttttt ggtcattttt cgatgggttg tttcaaaata tgccaataat
    11461 tttttgtatc cttccgtatt ttgtatgaga gacttaccga ggtgagccgc agttgcttct
    11521 tttgtcaggt catcttctgt cggtcctaat ccgccacata agagtatgag ttcacttctt
    11581 gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat tatctcctac aactgtttga
    11641 taataaactt cgatacctag atcagctagc tgttctgaca agaaagtggc attcgtattc
    11701 acgacttgtc ctagtaaaag ttctgttccg acagctatta tttctgcttt cattacttcc
    11761 cgctccctta tatagtatac gctgtatttt tatatttttt tatttaaaaa gagggcaagg
    11821 agctgtgaca aaaatcttgt cacagctcct tgttccaaat aaacagtgtt ctcctgccaa
    11881 aacctcggtc ataagtcaca atatacataa aacatgaaaa gctgtttttt gaaattatcc
    11941 cttgtgactc agagttagac ggcaggctca caacctctcc actaaacgat acttacattg
    12001 atcctttgaa cacaccgctg tttttgatga agtaatccac acctgaatag atagtaaaga
    12061 tcagacaagc ataaagcatg atttgatcta ctggaatacc gatcaggctg aatgggatat
    12121 tattgataaa cagcaagata atagcaacca tttgtgtagc cgtcttgact tttcccggcc
    12181 aagctgcagc catgacttct ccatgttcga caagtagtaa acgcaagcct gtgacggcta
    12241 attcacgaca gacaatgatc gctactaccc aaccaggggc ctttccttgg tcaaccaaca
    12301 tgataaaagc agtcataact agcattttat ctgccaacgg gtccgcaaac ttgccaaaat
    12361 tagtgacaag gcctcgcgca cgggcaattt ttccatctaa ccagtctgtg atactagcta
    12421 aagcaaaaat aactgcacct actagctgtg tgacagccag tgtggtgtct cctacgttaa
    12481 attgtcccca atctaaaggt aaacctacaa ccaaaatgaa aatcgggatc atacaaattc
    12541 taagtacagt taatttattt ggtaaattca cactatcgac tcctttcttc cctattctac
    12601 gtgactatca gcgaagtttc caccgatatt atttactttt ccaaatgaat ttattcaaag
    12661 gagtattttt attgaaattg atctttttga attaacgaag aaggaccgcg acacagattt
    12721 gtcacgatcc ttctttttta tacctacaag taaacagatg cgtaaactaa tttatacgat
    12781 cattctgcgt attgaagagt aaggttcaca ttttttggac tctgattgtt ttcccctgga
    12841 ttaactggta cggattctcc attcgctttg atcgttgcat tgcttgctgc accaatcgtg
    12901 atggtcgcat ttgttgctgt ttccggtaac acggtactct gtgtttcatt tgctgccagt
    12961 gtcccttgat aaacataggc attgtctact agcacaccta accatacacg attgacagca
    13021 gtaaattcaa gggttacagg cttggtagcg tctgtgatat ttactgcgat ggcactgcca
    13081 gaatcttctc ctgtagctat tgccattttc ttttcttcac ttgattttgt tgttgattct
    13141 actgtagaag actctttggt ctgcgtgctt tgctctgagg aagaggactc ctcgactgat
    13201 ccttctactt taagcgaaga agctgtgtct ccgattattg gatcagcttg acgatcttgc
    13261 caagtcatat agcccacgac aacgacaatc gccaaagcaa ccaatcctaa taaaatgaca
    13321 ggaagacttg tccaaaagcg acttggatgc gattcttcct gatgcattgc ttttctagaa
    13381 gtacttactg tttcaggctg cggacgttca gggaccgatc ctgtcaattt gcttttccca
    13441 tcaaaaacat cgactaaatg gtctccatct tctcctacag cttcagcgta ttgccgaata
    13501 aatgcccgca cgtaaaattt accaggcaat tggtcaaaat catcattgtc gattgcttcc
    13561 aaataacgtt tttgaatttt tgtaatctgc tgtaattcat ctaaagatat ttttttattc
    13621 aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag ccactcgttc atcttctcca
    13681 gtctgtttga ttctccgaag catctgctgt tgcaacgaaa gacgttatgt ttgggtgctg
    13741 tgacaaaagt catgtcacag ctcctttttt ccgaataaac attgttctcc tgccaactcc
    13801 tcggtcacaa gtcacaatat ccaataaata taagaagctg ttttttgaac ttgttccttg
    13861 tgactcggag ttaaacggca ggctcacgac ctctttctta aacggtgttc aatcagctgt
    13921 tcctcggtat tagctcaaaa accggcaaaa tataaggaga tatttttccg tatgtttctc
    13981 taatactcag gtcaaaacgc tgatttcaca acctcttcct taaacggtgt tcaaaagctt
    14041 gcatctgcgg taataagtcc aaccaagtta aaaacattaa gaagctgttt ttacttggct
    14101 gttcttatta cttgatgcag ggcagctttt tcacaacctc ttggtacacg gtgttccaga
    14161 agtagcttct tcggcaataa gccgaaatat cacaaaaatt tgagaagcaa ttttcgtaaa
    14221 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctttgatt acttcgtttg
    14281 attttatttt aatataagtt gattaaaaga gagcatgatt tgttttattt tctcactaat
    14341 ttttccaaca caactaagaa taccataaaa gctgattttg gaggaggttc ctactacaag
    14401 tctaaaacga aatttaagac ttttcctcat aaaatactgg tttattttgg tctcatgtaa
    14461 aactcgctga aggcctcttc acttataaat gcttccccag ctgccagtac gtctttcatt
    14521 tggatcgaat cgatgatttc aggaagatca aataaagttc gatcgccaaa taaactttgc
    14581 gtaaattgat tggcaatata ttcgatcgaa tttaatgatt ggaaatattg tccaagcatt
    14641 ttcttcttca gcaaatcgag atttgtttct gacacttccg gatcatcagc gaatccaaga
    14701 atgatttctt tgactttttc tgctgctttc tctggttcgt ccgtatctcc gctgaagtcc
    14761 gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa agctgtcatc gataattcct
    14821 tgattgtaca tcgctagata gtttcgagaa gtattgccta aaatcatttg gaacaacaga
    14881 ttgattgctg ttttatagcg taacagttct gttccttctt gcggcaatgt gtctaatcct
    14941 ttaataccaa gtacaaattt atctcgtgta atggctgctt ccaatgcact ttgtttgatg
    15001 atttcttttg tgttttcagg gaaataacgt acaatttctt gctttggagg gaaatttttt
    15061 gcttcttggt tttcccgaat aagtttcatc aatttctcag gctccatctt accaactaca
    15121 aataaaacca tattgcttgg ctgataaaac gtccgatagc acgtatacaa gtcttgagct
    15181 gtgattttgt cgatactttc aaccgtgccc gcaatatcga tatgcaatgg gtgtgtagga
    15241 tacaagttat ttagtatgcc aaaaaacatc cgccagttag ggtcgtcttc atacatttgg
    15301 atttcctgac cgatgattcc tttttccttg tttacagttt cttctgtgaa gtaaggtgct
    15361 tgtacaaaat cgatcagagt cgtcagattc ttttctacct gatccgtcgt agaaaacaga
    15421 tagctggttt tcgtaaaact tgtgaaagca ttggcggaag ctccttgttt cccaaataat
    15481 tgaaagacat caccatcttc tttttcaaac aacttatgtt ctaaaaaatg ggcaattcca
    15541 tctggaactt tcaccttttc tttttcacca tagggaataa attcgttatc gatcgaacca
    15601 taattcgtac tgaacaaacc atatgtcttg tgatagtcat ttttaggcaa caaatacacg
    15661 gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat tgatttgctc atattctgtt
    15721 ttattcattt gcatttcctc ctgctaaaaa gaagattgcc tgcaattcga tttgctcagc
    15781 tacttgttgt atctctttga tcgttacgct ctccatacgt ttcaaccatt cttcatcaga
    15841 taattttgtt tccggcagcc agctgtccaa aaaagaagct tcgattgccg cttgcggaga
    15901 gtctagagaa agcagatatt ggtttctcag cattgctttt gtctgagaca gttccaactc
    15961 tgttatttca ccatttcgca aactttctaa ttgctcgtga atcagatgaa gtacttgatt
    16021 tcggttcttt tcatcgatac cggtttggac actcatgaat cctcggaacg tatccacgct
    16081 actagaggca taataagcta gactttcttt ctctctgaca ttcatgaaaa gtttagagtg
    16141 aggaaaaccg ccaaataaac cgttaaagac cattagcgca aaacgttctg gttcatcgta
    16201 atacacattt gtctgataag ctaggttcag ctttgcttga acgattggct cacgtacttg
    16261 tcgttccttc acgatattca cttgaggttg ggtataaaac agatctggat aaactgtttc
    16321 ccgcgactca aatggtaatt gcccaactgc ttcagctgct ttgtccggat caacatcgcc
    16381 gacaacaaaa atgtcgattt gatcatttgc catcatttct tgatagtagg acgctagcga
    16441 actagcagtg atcttgtcca aatcttcgac tacgcccaac cctgggatct tttgttccgg
    16501 gctgttttca aaataaagtt cttgcaaggc ataggaagca aaagtttgtt tatcttcctt
    16561 caaactttcc aaatatagac gcagattgtt cttttccaag tcaaacgtca actgatcaaa
    16621 ctgatgattt ttaatatttg gataaaataa aacctctttt aaaaaatcta ccgcttgaga
    16681 aaaaaggttt ggatcattga catattcacc attcacaaaa ctgatccctg cgtttagcca
    16741 atgtaaattt cctttttttc taactgataa tccaaagctt gcgccataaa gctctgcaag
    16801 ttgcgcactt aactttgtct gatccgaata attcaaacta tttgtttcca acatgctgct
    16861 gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc gcagtaaaac ggataaaaat
    16921 ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga ttcacgcctt ctgccaattg
    16981 atacaccata tttatatgct ccttttcaca gttgattgcc cgcttactct tttaatgggt
    17041 caacctgcct tttttactta ttcctctgct tgaaatttat aagttcaggc aattcgccgt
    17101 tttttctaaa tgatgttaca ctttatagta tagcatagat gttttttatc aaaagaaaca
    17161 gacggctaaa agcgtatatt gtttttttat tttttatttt tcgatatact aaagacaaat
    17221 aaaagaaggg agggtcattc atgatcaaag aattcaaaga gttcatcatg cgtggagatg
    17281 tcttggatct tgctgtcggc gttgttattg gtagtgcgtt tacaagcatc gtaaaccaag
    17341 tggtgcaagg cttgatcaca ccactaatag gttggtttgt cgcaatgatc acaggtactt
    17401 ctgatttaga aggagcactt tcaattcttg actggtcgcc gacaaaaggc gtcacatttg
    17461 cgtttggtaa cgttattagt gctatcatta catttctgat cactggtttt gtcttattct
    17521 tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc caaagaagag caagtggaag
    17581 tggaagaaac tcctactgca gaagattatc ttagtgacat acgcgatctt cttgccaaac
    17641 aagtggcaga agacaataca catccagtag agcaaacaaa tctttccgat acttctaaat
    17701 aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc ggctttcttt ttttagattc
    17761 ttaacttgct acttgttcct ttttcatgtc gatatgtgga atatcatctt ccaaatacac
    17821 atctgagatt gccttgaatc caaacgactc ataaaaagct tgtaggtatt cttgtgcgcc
    17881 gatcacgatt ggtttttctg gaaaattttt ctggatcatg ttaatcgttt cttgaacaat
    17941 ctttcttcct aaaccatctt tacggttgtc ctgatgaaca aggacacgtc caaaatggat
    18001 actctccgct tcttcatata ctcgcgtgta ggcaaggatc ttcccatcag tatcttgaaa
    18061 aaacgtatgc caagcttctc catcaatgtc atcaacttct tgataaggac actgttgttc
    18121 cacaacaaaa actgcgattc gttctttgac gatcgcgtaa aactctgctg ttgacaattc
    18181 ttcaaatttc tttacctgat attccactaa gcaaacgccc cctgcttttt tattcttagc
    18241 ataccgattc gcacacaaaa aagcaaacga accttacgaa tctcaggctc gtttgctttt
    18301 taaactgctt cagttaaaaa ttcgatctca actgtccgag taagtacatc tgaatagctg
    18361 taagatactc gttcaaaaga attttcttca ggatccaaat caacaacgaa tactgaagga
    18421 taggtttctg ttaaaacgcc gcggcgttct gtttgacgct ttcttcctgt ctgagcaacc
    18481 aacatgattg ggctgccgat acgaccttct aaatctttct tgattgaagc taaagttgtt
    18541 ggcatacagt tcacctctac tatcaactat accacatatt tttaaaattt tcaagtttac
    18601 cataaaaaaa aaagaaatgc aataaaaata aataagaagc tcattaagat ttttctaaat
    18661 tattcctcat tataaaattt caagtgtttt tttattttga gttttactct cccgtaagcg
    18721 ctcattactt gcttgtgatc cattgctaaa cattcagcca tgtctacaaa atcaacgcct
    18781 tgcatataat aatgaaaaat tttagcttct atttttgaaa actggatttc gcaattttcg
    18841 attgcttctt ttaataaaag atgctgaatt gctaaaggtt cttgcaatgt gatctctgct
    18901 aaaaagtctg gtccttctgt ctctattttt tggtcgattg agacttcatc tttttgatca
    18961 cgacgcttca gcgcgttctg cttcctcaac agagaataga tttgattgct tataacgctt
    19021 ttataataac ttccaaagct aacacctttg cttttttcgt aagttctcaa tgcctgatat
    19081 aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat aatttcttaa ataataggtg
    19141 tttttcaaac gtagtaaaag tggaaaatat ttttgataca gggcagcaaa tgcttcttcg
    19201 tcaccattac gtgcagcgcg caataactcc tcagacataa aataattcct ccataaattc
    19261 gtttatggag gaatcttatc atcaatgatt catcgaactg attagaacga tatgttattt
    19321 attttttctc attaaatcgt ccaatttttg ggacaatttt tttaactgct cttcattcca
    19381 tggagaatta cgtcggaaat caataaaatg catatcttta ttttcttggg ctattgtttt
    19441 ttccgttttt tttatcgtct tatataattc ataagcagaa gttcgtaatg ctccttggga
    19501 aaagatcatc cactgttctg ctaaatcgct tgtcgccact gtcacttgtg tcaaacggtc
    19561 attcagctga ccagcgattc tctcgatata actgtcagcc gtttcgtctt ctttcgtaaa
    19621 aacaacatcc aactggtatt tcttataatt ttgttggatg cccggaacta gttgggcatc
    19681 gaagacaacg atgacttcca ttccttcata tttcgcataa tttgataaac gatgtaaaag
    19741 agcttctctt gcatccgcca ttttgtcctg ctttttaagc tggacaagtt ccggccacga
    19801 accaatcatg ttgtagccgt ctacaatcaa cagctgtttt ttcatggtct actccccctg
    19861 acgttgggaa aaagcctgat acataagcaa accagcagca acactggcat ttaagctctg
    19921 gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt tcttttttca atcctggact
    19981 cattcctttg ccttcattac caatgactaa agcaattgat cctttggcat tccatttttg
    20041 gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat cctgtttctt tgagctgttg
    20101 cacagcttgt accaaattcg tcacgcgtgc aacagggaca tgttcgactg ccccagtgga
    20161 agtctttgtt acgataggtg tgattccaac tgctcggtgt tttggtatga tcacaccatc
    20221 gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa ttgtgtggat cttcgatgct
    20281 atccaaaatc aaaaagaagg ggtcttcttt tttctctttt gttcgttcaa tcagttggtc
    20341 aagggataaa tattcataag gtgtaatcgc aagaacgact ccttgatgca cgccgtgatc
    20401 gctcaaagtt tctaattttt gtttaggaac ccatttgact ggcacagctt gttcacgtgc
    20461 gatttctttc agttcttcta ctttttctcc tcgtgaatct tcttgaagaa agagtttatt
    20521 ccctcgccct tgttgtagcg cttctactgc ggcatggtgc ccaaagacga aattatcttc
    20581 taatgcttct tcattttgat cagaaactga ctgatttcgt ccagtttgct ttttatttga
    20641 atatctattt tgttttttgt cttcaaatcc acgtttcgat tgatgtttgt ttgttctttc
    20701 tttacccatt tttttcacca actttctgaa tacaccagcg gatcaattct tccatccgct
    20761 cggtttgttt tgtcaaatgc agatagccca tgagcgcttc gaaccctgtt gcgacccgat
    20821 acgtcgtaac atcagcattt tttgcgctcg tatggctttt agcatttctt cctctgcgat
    20881 aataaagttc ttcttcttct gtcaaaagat gttcttccag catttgctga atcaaaaagg
    20941 cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg cagcttattg ggttttgttt
    21001 ctccttgatc aatcaggtgg tcacgaacat agatttcgta aatcgcatct ccaacataag
    21061 ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct tctcttctcc atcgggttcc
    21121 ttgcggtgta tcttctaata agatcccttt ttctttcaac aaatcacgaa tttcgtcgct
    21181 tcggacaaaa tcacgattgc ttcttgcttg atttcgttct tcgatcaatt gttcaatctc
    21241 ttcatctaat aactcttctg ctgtaaataa aataccgaag atcgaaagcc attgcgagaa
    21301 taaagcttct ccttgtgcca agacagcggc agaaactttc ggctggtcta gataagtgtt
    21361 cagccactta gcaagctcat agaccactgt gatcccattt gctgcattaa aatcatcgtc
    21421 catttcttcg atgaatcgct gttctaaatt ttctaacaaa gcgattttat ccgcatcatc
    21481 tgccaaattc tctgaggcag tttcttggcg gaatttcaaa ttgtcaaacg tattcttcag
    21541 tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca ctgtagcgga tcggacgacg
    21601 gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga tcaacgtttt tgatcatttc
    21661 atgtacagtg atgaaattcc ctagagattt actcattttt tcatcgtctt cgccaatcgt
    21721 tacatagcca ttgtgcatcc agtaattagc gaattttttc cctgttttcg cttcactttg
    21781 tgcgatttca ttttcatgat gaggaaattc taaatcttgc ccgccaccat ggatatcgat
    21841 cgtttctcct aaatgttttg ttgccataac ggaacattca atatgccaac cgggacgtcc
    21901 tttgccccat ggtgaatccc aagaaatctc gccaggtttt gcttgtttcc ataaagcgaa
    21961 atctaatggg tcttcttttt gctgctgttc ttcccctgta cgctgacttg ctccgacttc
    22021 cagttcatcg atggattggt cacttaattt cccataattt tcaaatttac gggtacggta
    22081 atacacatca cctgaagatt cataagcata gcctttttca accaacactt gaataaatgc
    22141 taaaatatct ggcatatgat ccatgactct tggatgcagt gtagcaggtt cgacattcaa
    22201 cgctttcgta tcttcttcaa atgctcggat gaaacgatca gcaacttctg gtgctgtaat
    22261 acctaattcg tttgctgtac ggatgatttt atcgtctaca tccgtgaaat tcgatacgaa
    22321 atcgacttta tagcctcgat attcaaaata gcgtcgaatt gtatcgaaag caatggtgct
    22381 acgagcattt ccgatatgga tataattata cacagtcggt ccgcagacat acataccgac
    22441 atgtcccttt tgtataggga tgaattcttc tttttttctg gtcaatgtat tgtagatttt
    22501 tatcattctg atctcctcac tttttctcct ttgattcgaa cgacttttgc tggtactcct
    22561 accgctgtag cgtcgtctgg aacatctttc aacacaacgg cacctgcacc gattttggcg
    22621 ttttttccga tcgttaccgg tcctaatatc tgcgcattcg cagagagcat ggctccttgt
    22681 ttaacagttg ggtgtctttt acccgtttct tttcctgtgc cgcctaatgt tacgccatgg
    22741 aataaaatca catcgtcctc gatttctgcg gtttcgccaa tgacgattcc cattccatgg
    22801 tcgatgaaca cgccagttcc aatcgttgct cctggatgga tctcaacacc agttaggaaa
    22861 cgccagaatt gagcattgat cttcgccaat aaaaagagcc ggtgctgata taaaaaatga
    22921 gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg tcaaaacgat ctcagctgtc
    22981 gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg ctcgggtaaa ccaactcatg
    23041 actttccact cctttcgtaa gaattttatg taggaaatca tcttcttata aagaaaagaa
    23101 gcgcctttta aataagtttc cttatcaaaa gacgctttcc gcgtggttcc acttttcttc
    23161 ataatcaatg attatctctg acagctaacg cctgttatac gttcttggct actttgtttc
    23221 ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga ccactcccag aggcatttcg
    23281 gcaaccattc attgatacct tgcaccaccc ggtatctctc ttgtaatgat cagctgctta
    23341 cttttttctg ttcatcgtgt ttagttcagt acttgattta aatgtgctaa tgatttttca
    23401 cgaccaagca attcgatcgt ttcaggcaat tctggaccat gcatttgtcc tgatacagct
    23461 acacggattg gcatgaaaag attttttcct tttacgccag tttctttttg aacagctttg
    23521 atggcagctt tgacagttgg agcatctact acttccattt gttctagctg tgctttgaat
    23581 gcgtggagaa cagtaggaac tgtttcacca gcaagtactt cttttgctgc ttcattcaat
    23641 acaggatgtt cattgaaaaa tagcgtagac agatcaacga tttctgcagc atagctcatt
    23701 tgaggttgat acaggctaac gatcttcttc acccattcga ttgtcttttc atctgggttt
    23761 tctgctacac gtccatcagc gattagataa ggcagacaca tgtcagtaaa tgcatctaga
    23821 tccattgctt tgatgtaatg gttgtttacc cattgcagtt ttttcgcatc gaaagctgcg
    23881 ggtgatttac ttaggcgttt tggatcgaac attttgatca attcttggtg ttcgaagatt
    23941 tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa agttgaacat tgcatctggt
    24001 agatagccta attcgcgata ttgttcgata aattgcaaga ttgattcgtc acgtttgctt
    24061 agttttttac ctgtttcact gttgatgatc aatgtcatat gtccgaaggt tggtggtgtc
    24121 cagccaaatg cttcatagat catcagttgt tttggtgtat tggcaatatg gtcatctcca
    24181 cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg caacagcaaa attataagtt
    24241 ggcataccat cgcgtttttg gatgacaaag tcgccaccga tattatcgga ttcgaacgaa
    24301 atagcgcctt tcaccatatc tgtaaatgcg tattctgtat tgcgaggcac acggaaacgt
    24361 acaacagatt ctagaccttg tgcttctttt tctgcttgtt cttcaggtgt caagttcgca
    24421 catgttcctg cgtaatgagg catttctcct cttgcgcgtt gtgcttcccg ttctgcttct
    24481 aattcttctt ctgtgcaata gcatttgtat gctcgattgc tagcaagcag ctgatcaatc
    24541 agcggttgat agatttcttt acgttcagat tgacggtaag ggccgtattc tccaggattt
    24601 tcaggagatt catcccaatt cattcccaac caagcaaggt tttccaactg gcttttttcg
    24661 ccatcttcga tattacgttt ttggtcagta tcctcaatac gaataataaa gtctccatca
    24721 ttgtggcggg caaacaaata attgaataat gcagttcttg catttccaat atgcaggtgc
    24781 ccagttggac ttggtgcata gcgtacgcgt actttcgtca ttttcttcct cttctttcaa
    24841 actagacttt gactaacagc aaaaattccg tcagttacgg cttctcaaat atcatttatt
    24901 taaagccact cagcacaaat tttacctttt ctaaggtaaa tagtaaagcc aactggttga
    24961 atttccttac cagttggctg aatttctttt ttattttgat tgtttagtct ttttctctgt
    25021 ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt ttagcaaaga tcatgcgtcc
    25081 agcagctgtt tgaagggcac ttgttacaac gacttcgata cgttcattca tgtaatgctg
    25141 tccatcttct acgacaacca tcgtgccgtc atcaaggtag gctactcctt gttggcgttc
    25201 tgtcccggct tttactacta gaacattcat cgtttcacca gggatgacga ctggttttac
    25261 ggcatttgcc aacgcattga tattaagaac aggtacattt tggaattcag agactttatt
    25321 caagttatag tcattggtta caatgacacc atctaaaagc ttagccaatt tgatcaattt
    25381 gctgtctact tcactgatat cttcaaagtc tccatcgtac atttcgacag atataccctc
    25441 ttctttttgc aaggcattta ggatatctaa tccgcgacga ccacgcacac gtttcaaact
    25501 atcccctgag tccgcgatgt attgtagttc gtaaagaaca aaattaggga tcatcaaggt
    25561 tccttctaag aatccagtct ttgtaatatc ataaatccga ccgtcaatga tcacgcttgt
    25621 atccaagatt ttatatttat ggaaattttc tcctgatttt cgttctaaca gttgactctc
    25681 aacttgacta gctgtttctt gctcgatttt tttgcttcgt gaaccaaaaa ctttcttcca
    25741 ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag cctaagatga tcatcagtaa
    25801 aatcggcaat acactattga cgaatggaat ttccatattg taaagagggg tcgagatcac
    25861 aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg aagagaagat aagtcaagct
    25921 gacctcactt agtttttgtt ctacttgttt gataatagct gagatatgtt tggccaacag
    25981 caaagataat aaaaagaaaa taagtgcgcc taacaggctg ttggtaaatg tattattcag
    26041 ccatccgttt gactgttgac cgattgcctg ccaagctgag ggtaaaaatg aaatacccaa
    26101 gcttgcgccg acaatgatca tcagcaaagt aatgacacgt ttttgcatcg tttttcctcc
    26161 gtttcgacaa tcatttatta gttagcggaa cacacgcttc aacgtttcag ccaaagttgc
    26221 tactccaact atctcgattc cttttggcgc ttcccagtta cctaagttgt ttttaggtag
    26281 gtatactttt gtgaagccga gtttttggac ttctttgact cgttgttcaa tatgactgac
    26341 acgtctgatt tctccagtca gtccaatttc accgatgaaa cattccgttg gctgtgttcc
    26401 tttttcttta tagcttgaag caatactgac agcgatagct aaatcaatcg ctggttcatt
    26461 taattttacc ccacctgcag cttttaagta agcatcttga ttctgaagaa gcaacccagc
    26521 tcttttttcc agcactgcca tgatcagaga tacccgattg aaatcaagac ctgttgttgt
    26581 tcgtttggca tttccgaaca tagtaggtgt gactaatgct tggatctcta ccaaaattgg
    26641 acgtgtccct tccattgcca caacgatagc agagccagtt gcaccgtcta ggcgttcttc
    26701 taagaaaatc tgtgaaggat tagcgacttc ttccaatcca tgctcatgca tttcaaaaat
    26761 cccaatctca ttcgttgagc caaatcggtt ttttactgct cgcaaaatcc gaaaactatg
    26821 atgttgttca ccttcaaaat ataacaccgt atccaccata tgttctaaca ttcttgggcc
    26881 ggcgatcgag ccttctttgg taacatgacc gacgataaaa atggctatcc catttgtttt
    26941 agcggttttg agtagttctg cagtggtttc tcttacttgg ctgacacttc ctgccacact
    27001 tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg ataacataat caggggagat
    27061 atgttcgatg gcacgactga tttcgttcat atctgtttca gcgtatagat aaaattctgt
    27121 gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc tctgcgcttt cttcgccgga
    27181 tacatataag acttttccgc ctatggctgc taattgctga gaaacttgta acagcaaagt
    27241 ggatttcccg atacctggat cgccaccaat caagaccata gaccctggca ccacacctcc
    27301 gcctaacaca cggttcagtt cttctaattt tgttttgatc cgaggttctt ttttagggac
    27361 gacgtctgcg atttttgttg gttttgcttt ttctcctgtc aaacttgtcc gagttctgcg
    27421 gtccgttgta tcttgttcga tttcttcgac catcgtattc cattttccgc aatttgggca
    27481 gcgtcccaag aacttaggcg acacataccc gcaattttgg caaacaaatt gtacttttga
    27541 tttttttgcc attgtaccgc ctccttttat cctaatcatg agacattaaa tcaagcttaa
    27601 gtatctttaa cgttttattt actggaagaa ccaaatccgc cttgacgttg agcagtcgtt
    27661 tgatcttgat ctgcttttaa aaacggcaaa aagatccctt ggccgatacg ctcgcctttt
    27721 ttgatcacta catccttgaa accaaagttg gtgaattgga acatgatatg gccttcattt
    27781 gcctcattat tgtaataatc actgtcaatc acacctactc cgtttgtcaa cactaaaaac
    27841 cgcttcaacg gattgcttga acggttagca agctgcaaaa attcatcttc tcccatgtaa
    27901 gctttgattc cagtaggcac gagggttggt ttcaattgtt tttcattttc tgatacatct
    27961 tttttcaatc catctgcgac tgtttccaac ccctttttcc agcctgcttt ccagatactt
    28021 ggaatcacga cctcttctgc tgcttcaaaa tcatagccag cagcatgatg tgtagcgcgc
    28081 tgcggtagat tgatattttg atctttatat ttagaaataa cttcaaatcc tctttttctc
    28141 accggttttc ctccctaaat aaactactcc tattctaaca tatccgaggc tgtaaaagac
    28201 tttctttaca aattcttcaa gaaattctgc tatcttaaag taaaccatta attcgttctt
    28261 tttccaaacg actgcaagtt gtacaaaaaa actatttttt acatgacttt actcgtcaaa
    28321 acaagtagaa tagaacgaaa ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa
    28381 gaaattaaaa atgcaaaacg aattattgtc aaagtaggaa caagtacatt gatttatccc
    28441 aatggaaata tcaatcttgc ggcaatcgat cagttagctt ttgtcctttc tgatcttaga
    28501 aatcggggaa aggaagtgat cttagtctca tcaggagctg taggcgttgg tatgcataaa
    28561 ttacatttgt cagaacgtcc gactttgatt cctgaacagc aagctgcggc ggctgttggt
    28621 caagccgaac tgatgaatat ctataatcaa agattcagtg tatataatca acagacggca
    28681 cagatcctcg tgacaagaga cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat
    28741 acattggaac agcttttgat tatggacacg atccctatcg tgaacgaaaa cgatactgta
    28801 gcagtggatg aattagatca tctgacaaaa tttggcgata atgatcagct gtcagccatc
    28861 atggcaaaac ttgtcgcggc agaccttttg atcgtccttt ctgatatcga cgggttttat
    28921 tcagataatc cgactacaaa tcccgaggct cagatgtatc gctatatcga taaaatcgat
    28981 gaaaagattc tggcacaagc aggtggtgct ggtagcacat ttggaacagg cggtatgcag
    29041 agtaaattaa aagccgcttc taggatattt gaagcaaaca gcgcaatgat tttagcaaat
    29101 ggaaaaaatc cggcaattat ttttgatatt cttgcaggaa aagaagtagg tactttattt
    29161 aaggaggact tgaaatgact gacttgatgc aattaggaca acaggcaaaa gatagcgcgc
    29221 aacaactagc acttatgcct acaaaccgca aaaatgagct acttgttcaa atggcacaaa
    29281 ccatcaaaaa gaatcaacaa gcgatcattg aagcaaatca taaagactta gaaaaagcag
    29341 tcgaaaacaa tatcagtgaa acgatgcaag accgcttgca gctgacacct gagcgaatca
    29401 atgccatggc tacagagatc gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag
    29461 atgagatgtg gacgaacacc gacggattga gaatcggcaa aaagcgcgtg ccactaggcg
    29521 tgatcggcat catttacgaa tctcgtccaa atgtcacgac agatgcagct agtctagcat
    29581 tcaaatcagg gaatgccgtt atccttcgcg gtggcaaaga agcattcttc tcgaatcaat
    29641 tattagtcca attattgcag caagtactgg tcagtgctgg cgaatctcct tatgccatcc
    29701 agtttgtcga tgatacttct catgaaactg caggacagtt gatgcgattg acagaatact
    29761 tagatgtgct gattcctcga ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa
    29821 cggtacctat cattgaaaca ggtacaggaa ataatcacgt atatatcgat aaagaagcac
    29881 agttatctat ggcagtgaac atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta
    29941 acgcggcaga aacattgctg atccattctg agatcgcacc ttttttcttg ccagctattg
    30001 aaaaagaact ggtagaacac ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc
    30061 tagagacggc tgctttagca gaagacacag attgggatac tgaatatcta gattatatcc
    30121 ttgctgtcaa agtggtcgat tctttggatg aagcaattgc acatatcaat cggcataata
    30181 ctaaacattc agaaacgatc gtgacagata gttatgcggc tagtcaacgt ttcttaaatg
    30241 aagtggacgc ggcggctgtc tatgtcaatg cctccactcg ttttacagac ggatcggtat
    30301 ttggttttgg tgcagagatt ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg
    30361 ggttaaatga attaacttcc acaaaatata tcatttatgg cgatggacaa atccgcgaat
    30421 aatgtttcgt gtgaaacatt attcccgcct tttgcctttt ttctgtaaag gttctatcca
    30481 actagaaagg tgtcaatatg tttgaaattt taatgatttt ttttatttac cttatttctc
    30541 tgaatatcgc tgcttttctg ggagtcggta ttttatccct tttctttcaa ttcaaaaaac
    30601 gaagcatagg gagtcaacgg gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag
    30661 ggctggttac acgcttgcac attagttaca tggttgcatt atgcttatta gcgacaatca
    30721 attactattc ttttttcgat cattcaatcg cttatacgat tacgctactg attgctggga
    30781 tttttcactt atcttataaa tatcagctga ataaaaacca cttgaatcgg actttccgct
    30841 aaaaaatatc cgtacgataa gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt
    30901 ttagaggctg tgacaaagat tttgtcacag cctctttttc catataaacg gcgttcgcct
    30961 accagctgcc tggtaaatct gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt
    31021 cgccatcata ataacgaagc agctcatcca tggacaagtt catcgttttt tctttttctt
    31081 cttccgctga ttcagccagt agctgttccc gatacacctg atacatacca gctatttttt
    31141 gctgcatcgc ttttctagtt tcaccttccg cattgattat tggcaattct ttgatatagc
    31201 cacgataaat caaccgatca tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga
    31261 cttcctgaaa atattcttcc agcatctatt atccccccat tctttttctt aaatgaattt
    31321 tagcttacct actgaaaatt ttccagtttc attattttga gaaaaaaagg gaatgtgaca
    31381 aaaatcgtgt cacatcccct ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc
    31441 ataagtcaca atattcaaca aacatgagaa actgtttttt gaaattgttc cttgtgattc
    31501 agagttaaac gacagtctca caacctcttg gtaaacggcg ttcaatcagc tgaccctcgg
    31561 tattagctca aaaaacggta aaatatgaga agctattttt ccgttttgtt tctctaatac
    31621 ttaggataaa acgctgattt tacaacctct gttaaacggt gttcaatcag ctgtccttcg
    31681 gtattagctc aaaaaacggc aaaatatgag gaactatttt tccgtttgtt tctctaatac
    31741 tcaggacaaa acgctgattt tacaacctcc tgataaacgg tgttcaaaag cttgcatctg
    31801 cggtaataag cccaaccaaa cgaaaaatat gagaagctat ttttgtttgg ctgttcttat
    31861 gacttgatgc aggacagctt tttcacaacc tctgatttct tattcttctt catttgtagc
    31921 aaaataatta cggtgataaa tgatacaaca tacactgata agaacaatgg cacctgcaaa
    31981 ataaaacgca tagctttcac caatatgttc accaatcaca cctgcaataa atggtgctaa
    32041 agctccaccg aaaaagcgga caaaactata gctggacgaa gcaacgtttc gttgcagacc
    32101 tggtatttcc atagcaatcg tcgtcaacag tgtattgata attccttgga aaaatcctgc
    32161 taacacaatg ctgatcggta caaggactgg catagaagtt ccagctccta ataacagcaa
    32221 acaaataatg aataagacaa atgctgctaa aattgtccga taagtcgtaa acttccgctc
    32281 aagagccggt gcaacgaaaa tcgaagcaat agccagaagc acaccccagc caaaaaatac
    32341 aaaaccgact tctaattcag aatagccaat caataaaaat ggcgcatacg caagtaaagt
    32401 aaagaatccg aaattatata acaatgcgat tagtccaact gaacgaagtt tatgattgct
    32461 taacgctttg acaccttccc aaaggcctac ttttctcttt ggtttctcag tttcaggtaa
    32521 aagtaccgct agggctataa aaccgcaaaa catcaaaaat gctaccccaa aaaatggata
    32581 acgccatgaa ccactgccca agaccccacc gactagaggg ccaacagcca ttccgcagcc
    32641 aagtgctgct tcatacatca tgattgcttt ttctgtgtta ccaactaaaa tactgacgat
    32701 cgttgctaaa gcagtagaga caaataaagc attcccgaca ccccagccgg cacgtaaccc
    32761 aatcagcatt ccgatattcc cagataatcc gccaagacca gcgaatacaa cgatgataaa
    32821 caatcctgtc atcaatgttt ttttcaaacc gatccgacta gataagaaac ctgtaaatag
    32881 catcaccaca ccagtgacta acatgtaact agtaaataac agggtcgttt cagctggcgt
    32941 agcgtttaac tgtgcagcaa tcgtttttaa gattgggtct actagaccca ctcccataaa
    33001 agcgatcata caggtagctg ctacaactag cgctgaactt ttcccctttg tttcttcatt
    33061 tgtttgttct tgtatatcta tttgattctc caatatttcc atccctttct ctctattagc
    33121 atcatacatg atttatttta catgtcaata tgcacatgta aaacgtggaa actttactgt
    33181 gatataataa agaaaataag acgagaggta ggatttttat gaatacttta gcctatatat
    33241 tattatgcgt gcttttatcc aaaccacgtt ctgggtatga attgaagcag ctgatcacaa
    33301 ttttttggga agcccatcac agtcagatct atacaacgct tgccaagtta gaccagcagg
    33361 gttatgtgac cgttttggac aaagacgagc actctcaaaa aaagatctac cacttgactg
    33421 atgaaggacg tgctcttgta gaggaatgga tcaaagagga aactcccgct cctacgcaaa
    33481 aagatgaatt tctagcaaaa atctatgctt tttcgacgct cgataaaaca acagctactg
    33541 gactgctatt cactagagaa caacagctga acaaaatcat gcaaaaaaat caaaaaaaat
    33601 tgaatgacct ctcgctagac aagaaacaag aatttggacg ttatgttgtg atccagcgca
    33661 gaattatgct ttgtcagcag gaactccaat ggtgtcagca agtaagagaa caaatgcagc
    33721 agttttttga gtaagggata aacagaacct ttctagaaat atctattgaa gagcaaatta
    33781 tcttcaataa aagagcaata cagttggaaa agaaaaagcg atccgaacga caggccaaaa
    33841 cctatcgttc ggatcactat ttttctagaa ctgcattcaa gagactatga caaaaagcat
    33901 gccaaggcct tttttcaaat aacagtgttc catcattcat tactttgaat tgcttttagc
    33961 acacttcttg aaagcgggcc aacgatgatc aactgcaacg gtaaagcggc aatcacattc
    34021 attttccaag cattcatata agcagagaat aattgatcag gtattccttg ttccatgatc
    34081 acaccgaata aagacataca tgtcaccatt cctaaaacca tcaagcatga aatagttaag
    34141 atcatttgga ccttgctttc tggatttact ggtagtttaa aggcaatttt tttcgccagc
    34201 acaccgatga tgaagacatc caaaatgaaa gccaccacga atccaggcac tagaccaatc
    34261 aacaaacttg ataaatgaag atcttgatgc agccataaat tatacgcgct catccccaag
    34321 accatcaaaa aacacatcat tgttgtaaaa attattcctt cttttttatt tgtaggcaat
    34381 ctgtaccact cctttaaatt tgtcacgaaa atcatcgtag cacatttaaa aatttctcgt
    34441 aagtactttt tttattttct gcccttttat ttcataaact tcatcagccg tacgctcgac
    34501 aaagtaagaa tcatgagaag taaacaaaat cgttcccgga tatatttgca ttagcttctc
    34561 taaagcttcg atcgttgcca tatcaataaa attagtcggt tcatctaaca gcaacacatt
    34621 acttggctta gtaaaaagca aagcaatcgt cagacgggtc gcttctcccc cacttaatgt
    34681 acataacggt ttcgtcaaag tttcctgagc aaaccctaag tgattcagca aactacggac
    34741 aagcgattct gaaaactctg tttgtctcat gacaaaacga atgatggatt cttcagatgt
    34801 catttgataa cccttttgct gatatacttg aaagcttact ttcggagaga gaaggattcc
    34861 ttctccttgt ttgcggatat gttctaataa ggttgtctta cctgaaccat tttcaccgac
    34921 aatcgcgatt tttttaccta aagaaaactg aaaatcacca tttaccagta atgttctccc
    34981 acttctttcc aattggacat tttgtcccat gatgggatag cggctgtgga gttcaagaga
    35041 ttttggtact ggaaaacgga tttgtttcat ctgctgcggc ttggttgttt ctcctatttt
    35101 ttggagtctt ctctctaatg ttttcgcatt cttttggatg gctttttgta cactgtcttt
    35161 ttgtttagag gaagacaacc gatccagtct gatttgttgc tttttctttt tactggacac
    35221 tttttccaac ttttccgctt gttttttctt ttcttggatc gatttttcca atcgtttttt
    35281 ctctttcatg atcgactctg cttctcgtag ctgagtcttt ttctccaatt ccttttgagt
    35341 gagataggca ctataattcc ccgaaaactc tcggatttct ccgtctttta cttcccagat
    35401 tttcgatgct agctcatcta gaaaatagcg atcatgacta acaaaaacca gcgtaccata
    35461 ataataacga agctcttcaa tcagtcgatc aatacttttt ctatctagat gggttgttgg
    35521 ctcgtctaga agcatccctt gttcataaga agaaagaaga cgtatcaact cgatctttcg
    35581 ttcttcccct ccgctccatt tccgctctga agtttcttcc agtcttaact ggaaatgact
    35641 ggctaattcc aaatttaggt cctttgcttc agcgagatag gttaattgag gcaggtagtt
    35701 gaaggtgatc tctgtttgga ttttcccatg atcgggaaaa agttcaccgg ctattagttt
    35761 gagcaaagtc gatttaccga caccattttc tcctatgatc cctatacgat cttgctgata
    35821 gacacgaaga gaatcaatag atagttctag ctggttgcca aagctgactt gtagatttgt
    35881 tatatttact gctaaatttt ccatatgttt tacactccta tttcatgtaa aacaacaatt
    35941 atcgatacta aaaaagagca gaaaaatctg tttgcaaaaa aactgcatca catcttttcc
    36001 tactctgaac gaaataacta tgactaaatc accaaatctg tcatagcttt cacctattct
    36061 actgtcgttc gaaaaaatgg acatactcca tccatgaaat tgttgtttat acatgtcttt
    36121 tgaatttatt tagttgttat tcaaaagttc gaaacgtaat ttcatcgatg cagtcatttt
    36181 tccgacctcc ttttttatta ggttaccaca cacgattcaa aaaaagcaat agctactgaa
    36241 tagtaactga aaacctttta ttcgaacata gtgatctttt ctggtgctaa acgaacagac
    36301 tcgtaacctt cttctaccgc ttttcctatt gataaaataa taactggaac ataacggtct
    36361 ttatccaaac caaatgcctc tgccaactga tctgcttcga atccaccaat tggatttgtt
    36421 tcatagccat gatcacgcgc aactaacatg aattgcatag ccgctaagct agaatcgact
    36481 ttgactacat cgttcatttc ttctcttgaa aaactcttgt aataaggaat gatcgcagct
    36541 aattgttgat ctctgacttc ttttggcatc ttccctgatt cgtaagcttg gttgtagatt
    36601 tcttcgccat attcgtaaca ttgcatatcg ccaaaaatca tgaccattgc agaagaagta
    36661 tcattttgtc tcgtattgaa acggatcaaa ggacgcaaga tttcttttgc ttcctcgctt
    36721 tcagcgacca caaagcgcca tggtttcata ttgacagaag aaggtgcagt cgtcgcttct
    36781 ttgatcattt ccagcatttc ttcgtgtggg attttgtatg tttcatcata tacgcgaacg
    36841 gatcttctac cagtcataat tgttgaaaaa tcattttttt gttccattat tattaacctc
    36901 tttcgtttct gttgttatat tgcaaagttg ggtattcaat ttatctaaaa gtgttagaaa
    36961 ttgtttcatt tctacatcgc tgaaagccgg ggatagcacg gaacctgtat ctttatggtc
    37021 catttcacaa tgcttgattt tcttctgggc tttttctgtt agcgctacat acacttcccg
    37081 gttgttctga ggatttctct gacgcgaaac atagccattt gcttccaata ccttcagatg
    37141 acgagtaact gctccttgat caatctgcaa cttttcttgc aaactgcttt gtagcaatgg
    37201 attttcttct ttcagtctca tcatgatctc gtatctagta agacttatac cgatattttc
    37261 ttcaaagatc tggacgattt tttgatcggc taatttgatt tgatataaaa ggtgactaat
    37321 ctcttctaaa gacataattt cctcctttct aataattgac tcgtcaacga ttgacttgtc
    37381 aataatagaa taaaaaaaga aaaaagtcaa agaatcagct gtgtaataaa aaaaacgctc
    37441 tcaatagaaa agtatagaga aaagaaattt tttgtagaat gaacggaaaa aatgataaag
    37501 aaagcacaac gctaaaacca agattattga ttcttatcaa actatagaag atagctcttc
    37561 tgcagtagat aattttacac cgccaaaaat ttccgaaatc gccttttgca agaatcccgt
    37621 cgaatattag aaattggatc agtatgctta aacagtaata aaaaagttat gacaagatgt
    37681 tgtcacaacc ttttttgagt aaacggtgtt caaaaacttg catctgcggt aataagttca
    37741 accaaacaaa aaacatgaaa atctgttttt gtttggctgt tcttactact tgatgcaggg
    37801 cagctttttc actacctctt tttaataacc ataaaatcct attccttcat atctccagcc
    37861 catcggtgtt agccaatctc tttccttagc actcaaggta tagtgatggg taccagtttt
    37921 ttcatttgga ttaaatagtc gataaattgg cttggaacca ccagaataaa aacttagacc
    37981 ttcataacgc caaccgattt ttgttaaaga gttgatctca gattgtgcca ttgtgtaatg
    38041 atggtctcca ctgtaaggat tgtataaacg gtagacaggt tgtccgctgt ttggtgcttg
    38101 ccaagcaatc ccttcatatt cccagcctac agttggcaga taattttttt ctccggcatt
    38161 caatgtatat aaatgttctc ctgtatgacg gttatacaga cgatacatcg gagaagttga
    38221 agttggtgct gtatcatact gtgtcagatt ataggtttcg attacattgt tcaatgtaga
    38281 accataatta ggcgcagtgg cataacgacc tgttagccaa gccgtagcat ctttgtaaga
    38341 gttcgtattg cttttccatg caccggaata gtagtaaacg cctggctgaa aagaagtcgt
    38401 cttcaatacc gttacgttat cttgcaatga ttctttgtac gaaggatatt ttctaaaagc
    38461 ggcattcacc gtaatccatt gcccattcac atattccgaa gtaggcatcg taacagattg
    38521 gccattatag cttcctttaa tcccaaatag attgtaatta ggggcagaag ccaaagtact
    38581 gttcccccag ccactttcaa cgatggcttg agcaatcatg actgatgcat ataagtcatt
    38641 ggcacttgcc aaactttgtg cagttgaggc aatactatca atgaacgctt gttgagaatt
    38701 agcagatgag cgagcataaa aagcatagcc agtatagttt tcgaattcag acaaatctgc
    38761 ttgatgggta tgtgtcaatg tttgcccatc tgtcaattgg ttcgtgattt cttctgcatt
    38821 ctctggattg ttctgagctt ctgctgatgt actgtcttcg gtttgtgttt gttctgttgt
    38881 ttcagttgtt ggttcttcgg ttgaaattgt attttctgag acagtagctg actgcaaagt
    38941 actttctgta tcgttttcaa ttcctgtttc tgaagaactc gatgtctgag ttgcgacagt
    39001 ttctgtcgat tcttcagcta gcactgttgt gttggcaact gctagatttc ctaaaagaat
    39061 aacgctaaat aataaattct ttgctttcat cttttttctc ctttgaatta tgaaactttt
    39121 cctaaataac agtgtaacta aaaaagtaaa agcaagtcaa tgtccatatt taaataacat
    39181 ttgtttcttt ttaataaatt tgctttttct gcataaaaag acgtaaatga agaatattct
    39241 tctccattca cgccttaaat tgatggtcgt ttgaataact atttaacaag taattctctc
    39301 tggatattat ccgttatgga ttatacttcc tgtgtcgtcg gacggataat catgttgctg
    39361 acagacatcc ggtctggcgt ttctatcgca aatgctacag cagaagctat atcttcagat
    39421 gttaatcccc attctttttg cgcttcatga agttcaagag cagccttttt gtcgctgatg
    39481 gtttgatata attcagtttg aacagctcct ggagagatga tcgtagattt gatgttattt
    39541 tgccgttgct cttgacgtaa tccttccatg attgcacgga cagcaaattt tgttccgcaa
    39601 taaacggcgg aatcaggata aacaacatgt cccgccacag aatcagttga aatgatatgc
    39661 ccagattttt gtttttccat gattggaagt acggcggaaa ttccatttaa cacacccatg
    39721 atgttgatat caagcatatt ttgccattca tcacggtgac cttcaactaa tggagcggtt
    39781 ggcatgattc ctgcattatt gtacaataca tcgattcgac cgaacttttc catcgctaaa
    39841 tcgataactg cttggacttg tgcaaaatct cttacatctg ctgtttgaat atatagctca
    39901 gcttctggta aagattcttt tattgctttt aggcgatctt ctcggcgtgc agctataact
    39961 agtttagccc ctttttctgc taatagcttc gttgttgctt caccgattcc gcttgaagcg
    40021 cccatgatga caatcacttt ttctgataaa tcattcattc tattctcctc ctgttttgaa
    40081 aaatctcttg cttatactca ctatctctta tttcatctta ctgttcaaat aatccgttcg
    40141 acaggtttga cgattttggc agggattcct gcaacgatgg tattgtctgg gacatctttc
    40201 gtaacagttg cattcgctgc aacaatggca ttctcgccaa tcgttacgcc tggcagaata
    40261 gttgctcctg ctccgatcca agcatttttc ttgatgtgga ttggctttac tgacaagcct
    40321 cttcgttctt ttggatcggt tagatggttc accgtgatga ggttgacacg aggaccaatc
    40381 aatactgaat cttctaaaca gatgccacct aagtcaacga acatcacttg acgattgata
    40441 aagacatttt tccctatact aatatgtttg ccgaaatctg tataaaaagg cagcagaaca
    40501 gtcaccgtat catctacttt gtcactaatg atattttcta gccgtttgcg gatatctgct
    40561 ggttcatggt acccagtatt cagcgctgct aaaggctctt cgttttccgc ttggatctga
    40621 tggatcactt caaagagcgg atccgttact cgaatctctt tattgacgat ttgtttttcg
    40681 atgaggcttg tcattttgct ccctcctttt atccttactt tataacggac ggcttatgat
    40741 gtctaatact tataaaacat acttaactat tccttttaac tatacttagt ggtatgcttt
    40801 tttaaaaaca ggagggaaaa aatggatatt cgtatactga cgtatttttt agcagtagct
    40861 agagaaaaaa atatcagccg agcagcaaaa tctctgcata tcacgcaacc tactctctca
    40921 aaacaactga aagatttaga agaagaactt ggtgtgacac ttttcactcg aggctcccga
    40981 gagatacagt tgacggattc tggtcgatat ctttattcaa aaggaaaaga aatcttgtcg
    41041 ttggtagaaa aaacggcgaa caatttaaca aatgacgcaa tcatctctgg agaaatctac
    41101 attggcggag gcgaaactaa agcaatctct ttcatttctg atagccttca ttcgttgatc
    41161 aaggcgcatc ctgatatcca gtttcattta tatagcggga atgcagatga tatcatagac
    41221 aaactggata gcggattact tgattttgga ctcgtaatcg aaccgacaaa caaacaaaaa
    41281 taccagtcat ttcgactgcc agcccaagat cgttggggac ttttgataca tcggaatcat
    41341 cctttagcaa agcaaacagt catccatccg caggaactga aaactgttcc tttgatcatt
    41401 tcccggcaaa cgtcagtgga cagtcaacta gctgaatggc taggtttcag catagatcag
    41461 ttatcgatcg tcggaacata taacttattg tacaatgctt ctttgatggt cgataacggg
    41521 acttttggtg cattagctct agatgggatc atcaatactt atgggacgga aaaagtattc
    41581 atcccactcg atccgccatt acaagcttca ctgaatctca tttggaaaaa agaccagcct
    41641 ctttctcctg ctgctcgtaa atttttagat gtcctaacgg agaaatacag ttaagtagag
    41701 tttttttcct tcgctttcag ctttttaaca acgaataagt taagcaaaag atccaatgat
    41761 taaataacat aagtttctta atttgttcct tccttttatt tctgataatt ccagtaaatt
    41821 actataatta taaaaaaaga attgacaaaa cataactgtc tatataaaat tgattacgtt
    41881 aacaataatt cgtttcatgt gactagatca tactaggcat gaaaaatctc tgaggaacat
    41941 ggtttcccta ggaggttttt cgtgtctatt tttgtttggc cgcaatcaac tggtctagca
    42001 aacgtgaaaa ccaaatcttc aaaaagggtg cgcgcatgaa aatcaaaaag atctttaatc
    42061 aaaatgcagt attggtagat gacggtggac aggaaaaagt agctattgga aaagggattg
    42121 gatttgacag aaaaagaaat gatttgattt ttgatcgtga tgtcgaacgg atatttgtca
    42181 tggaaccaga aggtcaaatc aagttgcaaa atttacttaa tcagattgat gaacgctttt
    42241 tatttgcagc agaaacgatc attgatcatg cggaaactgt tttgatggaa aagttgaatg
    42301 aacatgtatt gatcgctttg accgaccaca ttgctttttc tgcggagaac attaataacg
    42361 gtatcgtcat ccgcaacaaa cttttaagag aaatcgaagt gctttacagt gatgaattca
    42421 gtattgctca atgggcagta gagtatctga caaaagagct tgggattcca tatacttatg
    42481 acgaagcagg atatatcgca atccatattc acagcggacg aagcggtcgg aacaataacc
    42541 atcgcagtat ccgtgaagtg acgattgtat ctgacatcat ccatttagtg gaatcagaat
    42601 tggaaacaga tatccattca gaaccatttt cactgaatta ttcaagactg gtgaatcatt
    42661 tacgactgct tctacaacga acccatgcgc aacaatatgc tgttttagac acggaaatcg
    42721 tagaaatggt caaacgaaaa tacccagaaa gctataagat ttctaaaaaa atccgtgtgt
    42781 tgctgaccaa agaataccag ctcgcgatca cgaaagaaga actgggttac ttggccatcc
    42841 atatcgagcg tttaagaagc gcaatcgtac aaacaaatgt taacaatcat gaggaggaaa
    42901 aaaattgaaa gcatatctac aacgaatggg acgttccctt caacttccag ttgcggtctt
    42961 acctgctgct gctttactag tagggatcgg aaactggtgg gcatcttata gtaacgacat
    43021 tgtcgctcat tttttacaag ctggcggtaa cgcagtttta ggtcaattac ctttgctgtt
    43081 tgccgttggg ttagcactag ggatgtcagt ggacaaatca ggtgctgctg ccttggcagg
    43141 tttggtcgca tttgaagttc cagtaaatgt tttaaaaact gattcagtag ctactttatt
    43201 gaatatcaaa ccagaatcag ttgatccatc ttttgctcag atcagtaacg tatttattgg
    43261 tattattgcc ggattgattg cggcagctct ttataatcgt ttccacgaag caaaattacc
    43321 gatggcttta tctttcttta gcggcaagcg tctagtgcca atcttggctg cctttgtcat
    43381 gttgctgatc agtgcagtgc tactgctcgt atggcctccg gtttactctg ctttagtttc
    43441 ttttgctaaa ttcattttga gtttaggctg gggtggtgca ggtctttatg gcttcttcaa
    43501 ccgtttactg atccctacag gattgcatca agcaatgaac tccgtgttct ggtttgacgt
    43561 agcaggaatc aatgatattg gtaaattttt ggcttctgaa ggaacaaaag gcgtaacagg
    43621 aatgtatcaa gctggatttt tcccaatcat gatgttcggg cttccagcag gagcatatgc
    43681 tatttacaga aatgcacgtc cagaaaacaa agcaaaaaca gcttccttga tgttagcagc
    43741 agcttttgct tcattcttta ctggtgtaac agaaccactt gaattctcat ttatgttcgt
    43801 tgcttggcca ttatatgtct tgcatgcaat tttcactggg atttctttag cagtgagtgc
    43861 attcttccat tggacagcag gatttgcctt tagtgccgga tttgtcgatt acttcctatc
    43921 tttgaaaaat ccgattgcga atcagccatt gatgttgatc gtccaaggat tagtaacagc
    43981 agtgatttac tactttggct ttaactttgc tatcaaaaaa ttccatctga tgactcctgg
    44041 ccgcgaggaa gcagatcttt cagatgacga tactgcaacg actaattcag acaataaata
    44101 tgcggcacaa gcaagcaaaa tctacgctgc attaggcgga gccgacaatg taacttcgat
    44161 cgataactgt actacacgcc ttcgtttgca agtcaaagat acaggcctga tcgatcaaaa
    44221 taaaatcaaa gccactggcg tacctggtat gaagatcatc gacggaaaaa atgcgcaagt
    44281 catcgtaggt acagaagtac aattcgttgc agatgagatg gcaaaactac atggtggtgc
    44341 tgcaacacga cctgcacaaa caaacaccgt ggttaaaaca gaaacatttg ccggtggaac
    44401 gatcgaacaa gatatttacg ctatcgcaaa tgggaaactg attccaatca ctgaagtttc
    44461 agatgatgta ttcgcggaaa aaatgatggg tgacggctat gctgtattac cagaaaatgg
    44521 agaaatcttc tctccaatcg ctggtacgat cacaaacatt ttcccaacga aacatgctgt
    44581 cggaatccaa acagatgctg gtatcgaagt cttgcttcat atgggcatca acactgttga
    44641 cttaaaaggc gaaccattca ctttatatgt aaaagaaggt caaaaagtcg caagaggtca
    44701 attgattgct ttagttgatc ttgccgctat tcaatctgca ggcaaaaaca cggatatggt
    44761 cgttgtattc acaaatggcg acaaagtcca aagcttagaa atcgaacccg cacgtgatgt
    44821 aaaagcaaat gataaaatcg gctctgtttc caacaaagcc tgagccatcg tcttccccag
    44881 acttttttat cgataaacaa acaggagctg agacaaaaat cttgtctcag ctcctttttc
    44941 cgaataaaaa cggcgttctc ctgccaactc cgcggtaata agtccagcca aacgtaacat
    45001 atgaagagct attttttatt tgactcttct tattacttga tgcagaacag cttttttaca
    45061 acctctgata aacggtgttc aaaaaccagt ttatcggtat gaagtcaaat ttatgcaaaa
    45121 atgtggaaag ccattttcac aaaatttttc ttcatactca aaactaatcg gctttttcac
    45181 aacctcttta tacattattc ctgatccaat atttctttta atgctgcttc aagagaagtt
    45241 aatggttttc ccaatacttt ttctaaatca tcagaggaaa tagctaactg atccttttta
    45301 atatcatgtt ggatcgctaa aaacatgtca gctgcttctt gagggaatcc ggcttctttt
    45361 agattttcga caaacgctgc atcacttgct tcaataattt ctaacttttt acctgtcgca
    45421 cgctctaatg ccttggctaa ttcttcatag tcagttagat tgccggataa ttctaagatt
    45481 tctggagagt cactttctac tagtactttt gctgcagctt ctgcatattc acgtttcaat
    45541 acccaacctg tttttccatt tcctgcagca taaacgaatt gaccagtttt taaagcctgc
    45601 ccaatgatag gtaattcatt ctctacatac caattattcc gtaaaaatgt atgggaaata
    45661 cctgaatcct taatcatttt ttcagtgaat tggtgatctg gcgctaatac actgatcgag
    45721 tgatcggcat ctgcaaaact tgtataagcg atataagata ccccagctgc ttgtgctgcc
    45781 ttcactacat tcttatgttc ggcttgacga tttcctggaa cgccagagac aaaaagcaat
    45841 cgatctattc ctttcaacgc ttctttcatt gagtctaaat catcataatc accaatacgt
    45901 atattaaatc cagcttcttt cagtgcttct cctttttctt cactacgtac tagtacatag
    45961 atttcatttt gtggaaccat cttttttaag aattccagcg cataaccacc aaaacctcca
    46021 gttgctcctg ttactaaata atgcatattt gccactccct attcttttta tttgttaaga
    46081 tttacagctg tacatttata aattacacct ttatcataaa gagtgtggta agataagtca
    46141 agcaaaaaaa atgaaaggat gacaactgat gaagtattct ctgcaattta gtgatgcaat
    46201 ccatgtactt gcctatattg aaatgtttca agatacccga tcactttcta gtgagatgat
    46261 tgccagtagt atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc agctcaaaaa
    46321 atccggtttg atctttacaa aaaccggaaa accgcagcct tcccttgcca aaagtccaga
    46381 tcagatttct ttactagatg tctatcgcag catcgaaggt aatacacagc ttatccaagt
    46441 cgatccaaaa acaaatccaa attgcgtcgt tggtgcgaat atccaagaag ctttagaaga
    46501 aagctacgct cggctgcaga aaaaggcaga agaagaaatg gctaagatca cacttgatag
    46561 tctgatacat aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca caggcctcgt
    46621 agagagattt ttataattct ctctactgtg cctgcatttt tctgatcgga atcgtctaca
    46681 taaattcttt tattctccgg gggacaaaag ccttgtcatg cccctttttc ctctctaatg
    46741 ctcaggtcaa agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt agctgaccct
    46801 cggcattagt tctgaaaaat gcaaaatatg gaaagctatt tcccattttt cctctctaat
    46861 gctcaggtca aagcgctact ttcacgacct ctatattatc atggtttatt gactaccggt
    46921 atccagattt ctcccatata tttcccgttg gtaactccca tttcgactac agcatttgga
    46981 cccccaacat aggcgtaatc ttctacttct cttaaaacat gcccaaaggc cgtattcgtc
    47041 aaatcatttc ttaattcttc aggcgattct gctaaagcga ttacaacaag atacgttcct
    47101 tttgggaatg ggatgattcg ggtagtgttt ggaacatcct gactcgtttg aacaccgaca
    47161 tagtacatca ttttcccgtt atagggttcg ttgactgtaa agaaataatg attttcagca
    47221 gcttgtttca atgcatctat tgttccatta ccgataactt tttgctcaaa tgcttgtttc
    47281 tctgcagcca aaccgacaaa atccgtattg ctactcttca attccacacc aatacccgtc
    47341 accgtaaaat ctggtttttc ttcgatccta tactttgcca tttctcccac cctttctatt
    47401 atattaagtt catcttacat aaaattaaag ggaaatgaca cgattacgct cttttttcta
    47461 gggatatttt tattttttaa ctattttttt gctttcactg caatccaaat ctcactgtaa
    47521 acattactga aatcctctcc ccattttgta aaagagatct cgggagcttc gactaactca
    47581 taatctgaag aaggaagcca ttctgcatag attttccccc aagtatcttg taaagtttgg
    47641 ggaaatggcc ctttattagg aaaaatggcc caagtgtgtt cttctatagc aagcatttct
    47701 aaatcagcgt acggattttc ttttgtcgta gcaaagccaa tcatgtgcat caaactgccc
    47761 ttttcttcca agcgggctcc atcaaatcca taagaaacgt tgattacttg atttggatat
    47821 aaatctccta acttatgcat ctcttccctt tgagtctccg tgatcgtctg cgctaacttt
    47881 tgtatttctt gattgactcc ttcaaatttg acaggaactt ttttcgccac acctgccaca
    47941 taaaactgtt ctttcttttc gatacgaaat tccatcgatt ttcctcctct gacatcaata
    48001 aaaaatgtca agcgtggaaa agatttttgt atgccgtatc tgctgatttc tgaaggcaga
    48061 tatcctgacc aatcacgaaa agctcttgaa aaaccttcga ctgtttcata tccatatcta
    48121 aacgccgttt ctgtcactct tgatccatct aggagatcgc gattagccaa agccaatcta
    48181 cggtttttga tatagtccat tagcgacatc cctgcaataa acgtaaagat gcgttttaat
    48241 tgatattcag agattcctgt attttcggct attattttat aggaaagtcc ttctgtcaaa
    48301 tgttcttcga tgtaatcgat caattggttc agatctttaa gcatatttat cttcctcctt
    48361 gcttgccttt ttatcatatt atcaaagaaa gtcaattact tgatgaatca ggaaccaatt
    48421 tagtcagatg tagaaaatgt tgtatggatc taaagaaaat tctcaagcag cgtttcgtaa
    48481 cggataattg gtattatctg cctatctatg ttttaccttt agaaaaataa attttgggga
    48541 agacacattc taagtaaaca aagcggtaaa aatatctttt attcccttag ttaatccgct
    48601 tactacctac tgtacaaagc aaattgttgt tcaaactgct gatcagaacg ctgcttggat
    48661 ttttctttgc gatacatcat acctctttta tcaattggaa caattattta tttctaacaa
    48721 aatcacttca tttttgtcag ataaataaaa aacaaaaaaa gttccatctt taaaacacgc
    48781 tattctttta ctttttctca cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa
    48841 ttcgagcaaa agtgattatc aaatatgtaa aatcagctac ttttttctta aacgtttctt
    48901 tatactaaat ataaataagt gtttcatgaa aggagaacta gatgaataat tggcttgagt
    48961 attttcctga aaacgttttg gaacgcggtt acagctatca tctgcacggt tttgtccgtc
    49021 atttgaacta tacaagcaag tacttgtccg caacagtttc tggcacggaa gattataagg
    49081 tagtgatcac ttgggatgaa aagaccaaca tgacttgtga ttgtctatat gccatagaag
    49141 gaaaaaaatg caaacacatg gctgctgtct tattcgctta cgaagaacgt ccaataaaaa
    49201 aatccaacta ctcactttcc gaactctcgt cccttgtttc ctccgctagt tcctctcttg
    49261 tacgggagct tttaacagag attcttatag aacatccgca atttatcgaa cgcttcaaag
    49321 ttaagatgcc atttcatgca ataaattatt cagataaatt aacaactatc atccataaat
    49381 acgatcatat tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc atggagatgc
    49441 gaaagttcat ccaagaagca gttgaatcgc tcattcagca aaatgcctat cttcccgcat
    49501 tcgaattgat caatgaagtg attgctactc tcgaaacatt ctattgggag cctgaggatg
    49561 aacgaaccct tcttctgata gaggactgtt attatttatg gaaagaatta ttagcagaag
    49621 cacctcatgc agaaaaaagg caaatgttct cttggttcgt ctgccaagtc gatcatactg
    49681 acgcctctta ttcaaaacgc tactccataa aaatcctgaa agaagacttt cgggaaaaag
    49741 aattttctaa ccaaaagaaa aaaatagata aaaaaactaa agaaacggta aagaaagacg
    49801 atttcaatga aaaatagcaa gtaaacgata acaaccatcc taaatccaca agaaatacag
    49861 cagaccttgc tttaaaaaca cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt
    49921 acccaatatt agtcatttga ctattaggta cgcgttttgt tttataagtc atgacaaatt
    49981 tccttcgttg ctgggtagat tttccggcat tgttgataga ttttatgata cttttctaca
    50041 ttttgtaaga gaggttccac tgcatctgta tattggataa aattctgaca acaactttca
    50101 ggtgtgtcga accactctaa tctttaaaga taaatattaa aataacgatt aataatatta
    50161 acagtcttta tttttcacca ccaaaaacct atctttgcat atactaaata gacgtatata
    50221 cacagatagg tttgtcttgt gaaaaataat gattatcctt accaaaaaat gtttgttttt
    50281 ctacatgttc gtacttatca aaaaatatga tccttattga aattactggc tatttatatt
    50341 ccaacgactt cttgtgttaa tttttgttat ctcatagtaa attatgtttc attttcactt
    50401 aatttagttg tcacgtgttc gtcaattcgt aaaaacggta aatatataaa cacacttgcg
    50461 gctataatta atatttgtaa aacagctgct cgccagtctc cggctgttgc taaaaatccc
    50521 gatataactg gtggcgtagt ccaaggaatt tgcactacta catgattaat taatcccaca
    50581 gcagttgcaa aatatgctac tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc
    50641 agactgatat taaatacaat aggcaatcca aaaataatag gttcgcttat attgaatata
    50701 gcgggagtta ccgataattt agcaaagtcc ttataatctt ttctacgact gaagataaat
    50761 attgcaatca ataaagcaat cgtatttccc gatccaccca ttaccgcaaa ggtatcttta
    50821 aaggccatat tgataatatg tggaatttgc tctttgtttg cataagctgc catattttct
    50881 tgcatattct gaatcaaaaa cggatctaac aatgaacctg atattactgc ttgatggata
    50941 ccaaatccaa aaaatagatt agcaatagat gtaattaaca aaaatcctgg taaaccagtt
    51001 gtaattttac ttaatggctc tgtaactatc gtggtaatta gcttatttat atccatatta
    51061 aacaattgaa tgattataaa tgacaataaa gcaaacgagg taatcgtaat aatgattggg
    51121 attaatacat taaaagactt taaaacagcg ggaggaatat ttccggatag ctgaatctgt
    51181 agtttcttat tattagaaag tttaataaat aattctgtag ccgctaatcc aacaaatata
    51241 cctacaaaca ttcctgaagc tcctgtatag gatataggta tcacacccgt cacttcaaca
    51301 gctttatccg cattttcagg agtaaacatc atctgcatag gtgttaatac aacaaatgtc
    51361 gaaatagcaa ctaataccgc tgcaatatga tttgtataat ttctgcttgc acacatatga
    51421 taagatactg ctgctacaac taataatgta gttatgctta atgtaccatt ggcaattgaa
    51481 gtaccaattt gttgtaacgt ggtcaatgta ctagtatcga caatttcccc catgaatcct
    51541 gttggttcta agatcactga attaataagt gtcacaaatc ctgctaacat cataaatggc
    51601 ataaatgtag caaacgcatc tcgcaaagtt cttaaatgga tctgattccc caacttagtc
    51661 gcaactatac caaatttttc aaatacaatg tccttcctgc tacttttgcc catatttttt
    51721 ctcctttatg cttattgtaa tcgattactt tt
//
LOCUS       DAZKOW010000006        51216 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000006 DAZKOW010000000
VERSION     DAZKOW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.006"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            330..1175
                     /locus_tag="ACG912_000377"
     CDS             330..1175
                     /locus_tag="ACG912_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0511725.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="ACG912_000378"
     CDS             1456..2676
                     /locus_tag="ACG912_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0511726.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="ACG912_000379"
     CDS             2673..5393
                     /locus_tag="ACG912_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0511727.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="ACG912_000380"
     CDS             5377..6321
                     /locus_tag="ACG912_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0511728.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="ACG912_000381"
     CDS             complement(6390..7400)
                     /locus_tag="ACG912_000381"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0511729.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="ACG912_000382"
     CDS             complement(7580..7924)
                     /locus_tag="ACG912_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0511730.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="ACG912_000383"
     CDS             complement(7917..8351)
                     /locus_tag="ACG912_000383"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFQ0511731.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="ACG912_000384"
     CDS             8489..9226
                     /locus_tag="ACG912_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0511732.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="ACG912_000385"
     CDS             9219..10430
                     /locus_tag="ACG912_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0511733.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="ACG912_000386"
     CDS             10634..11416
                     /locus_tag="ACG912_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFQ0511734.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="ACG912_000387"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="ACG912_000387"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFQ0511735.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACG912_000388"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="ACG912_000388"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFQ0511736.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="comGA"
                     /locus_tag="ACG912_000389"
     CDS             14132..15061
                     /gene="comGA"
                     /locus_tag="ACG912_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFQ0511737.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /gene="comGB"
                     /locus_tag="ACG912_000390"
     CDS             15018..16067
                     /gene="comGB"
                     /locus_tag="ACG912_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFQ0511738.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /gene="comGC"
                     /locus_tag="ACG912_000391"
     CDS             16064..16342
                     /gene="comGC"
                     /locus_tag="ACG912_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFQ0511739.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /gene="comGD"
                     /locus_tag="ACG912_000392"
     CDS             16317..16787
                     /gene="comGD"
                     /locus_tag="ACG912_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFQ0511740.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16759..17106
                     /locus_tag="ACG912_000393"
     CDS             16759..17106
                     /locus_tag="ACG912_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511741.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            17069..17494
                     /gene="comGF"
                     /locus_tag="ACG912_000394"
     CDS             17069..17494
                     /gene="comGF"
                     /locus_tag="ACG912_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFQ0511742.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /gene="comGG"
                     /locus_tag="ACG912_000395"
     CDS             17466..17825
                     /gene="comGG"
                     /locus_tag="ACG912_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFQ0511743.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="ACG912_000396"
     CDS             17997..19004
                     /locus_tag="ACG912_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0511744.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="ACG912_000397"
     CDS             19029..20213
                     /locus_tag="ACG912_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFQ0511745.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACG912_000398"
     CDS             complement(20650..22233)
                     /gene="abc-f"
                     /locus_tag="ACG912_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFQ0511746.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22338..22466)
                     /locus_tag="ACG912_000399"
     CDS             complement(22338..22466)
                     /locus_tag="ACG912_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511747.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22811..22954)
                     /locus_tag="ACG912_000400"
     CDS             complement(22811..22954)
                     /locus_tag="ACG912_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFQ0511748.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="ACG912_000401"
     CDS             complement(23177..23656)
                     /locus_tag="ACG912_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0511749.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="ACG912_000402"
     CDS             complement(23729..24013)
                     /locus_tag="ACG912_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511750.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="ACG912_000403"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACG912_000403"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="ACG912_000404"
     CDS             complement(24357..25643)
                     /locus_tag="ACG912_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFQ0511751.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="ACG912_000405"
     CDS             25781..26452
                     /locus_tag="ACG912_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFQ0511752.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="ACG912_000406"
     CDS             26418..26906
                     /locus_tag="ACG912_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFQ0511753.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="ACG912_000407"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="ACG912_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFQ0511754.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="ACG912_000408"
     CDS             27867..28631
                     /locus_tag="ACG912_000408"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFQ0511755.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="ACG912_000409"
     CDS             28864..31077
                     /locus_tag="ACG912_000409"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFQ0511756.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /gene="dtd"
                     /locus_tag="ACG912_000410"
     CDS             31088..31534
                     /gene="dtd"
                     /locus_tag="ACG912_000410"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0511757.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="ACG912_000411"
     CDS             31705..32964
                     /locus_tag="ACG912_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFQ0511758.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="ACG912_000412"
     CDS             complement(33053..34000)
                     /locus_tag="ACG912_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0511759.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /gene="hisS"
                     /locus_tag="ACG912_000413"
     CDS             34394..35686
                     /gene="hisS"
                     /locus_tag="ACG912_000413"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFQ0511760.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="ACG912_000414"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="ACG912_000414"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFQ0511761.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="ACG912_000415"
     CDS             37579..38454
                     /locus_tag="ACG912_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0511762.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="ACG912_000416"
     CDS             38720..40603
                     /locus_tag="ACG912_000416"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0511763.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="ACG912_000417"
     CDS             complement(40678..40962)
                     /locus_tag="ACG912_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0511764.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="ACG912_000418"
     CDS             complement(40952..41686)
                     /locus_tag="ACG912_000418"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFQ0511765.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="ACG912_000419"
     CDS             41815..42438
                     /locus_tag="ACG912_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFQ0511766.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="ACG912_000420"
     CDS             42630..43751
                     /locus_tag="ACG912_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFQ0511767.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="ACG912_000421"
     CDS             43758..46886
                     /locus_tag="ACG912_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0511768.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="ACG912_000422"
     CDS             46905..50099
                     /locus_tag="ACG912_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFQ0511769.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="ACG912_000423"
     CDS             complement(50245..50952)
                     /locus_tag="ACG912_000423"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0511770.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16760 a         8946 c        10638 g        14872 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaat taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatt ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gtgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ccagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttat
//
LOCUS       DAZKOW010000007        48048 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000007 DAZKOW010000000
VERSION     DAZKOW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.007"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            276..647
                     /locus_tag="ACG912_000424"
     CDS             276..647
                     /locus_tag="ACG912_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFQ0511771.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(921..1613)
                     /locus_tag="ACG912_000425"
     CDS             complement(921..1613)
                     /locus_tag="ACG912_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0511772.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1616..2401)
                     /locus_tag="ACG912_000426"
     CDS             complement(1616..2401)
                     /locus_tag="ACG912_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0511773.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2638..4038
                     /gene="lacG"
                     /locus_tag="ACG912_000427"
     CDS             2638..4038
                     /gene="lacG"
                     /locus_tag="ACG912_000427"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFQ0511774.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4063..4380
                     /locus_tag="ACG912_000428"
     CDS             4063..4380
                     /locus_tag="ACG912_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0511775.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4393..6066
                     /locus_tag="ACG912_000429"
     CDS             4393..6066
                     /locus_tag="ACG912_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFQ0511776.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6080..7711
                     /locus_tag="ACG912_000430"
     CDS             6080..7711
                     /locus_tag="ACG912_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFQ0511777.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8202..9767)
                     /gene="guaA"
                     /locus_tag="ACG912_000431"
     CDS             complement(8202..9767)
                     /gene="guaA"
                     /locus_tag="ACG912_000431"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFQ0511778.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9826..10749)
                     /gene="coaA"
                     /locus_tag="ACG912_000432"
     CDS             complement(9826..10749)
                     /gene="coaA"
                     /locus_tag="ACG912_000432"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFQ0511779.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10945..11550
                     /locus_tag="ACG912_000433"
     CDS             10945..11550
                     /locus_tag="ACG912_000433"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0511780.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11858..12874
                     /locus_tag="ACG912_000434"
     CDS             11858..12874
                     /locus_tag="ACG912_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0511781.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13015..14316
                     /locus_tag="ACG912_000435"
     CDS             13015..14316
                     /locus_tag="ACG912_000435"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFQ0511782.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14362..15024
                     /gene="deoC"
                     /locus_tag="ACG912_000436"
     CDS             14362..15024
                     /gene="deoC"
                     /locus_tag="ACG912_000436"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFQ0511783.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15045..15443
                     /locus_tag="ACG912_000437"
     CDS             15045..15443
                     /locus_tag="ACG912_000437"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFQ0511784.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15509..16591
                     /locus_tag="ACG912_000438"
     CDS             15509..16591
                     /locus_tag="ACG912_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0511785.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16803..18365
                     /locus_tag="ACG912_000439"
     CDS             16803..18365
                     /locus_tag="ACG912_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0511786.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18358..19491
                     /locus_tag="ACG912_000440"
     CDS             18358..19491
                     /locus_tag="ACG912_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0511787.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19491..20447
                     /locus_tag="ACG912_000441"
     CDS             19491..20447
                     /locus_tag="ACG912_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0511788.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20546..21712
                     /gene="deoB"
                     /locus_tag="ACG912_000442"
     CDS             20546..21712
                     /gene="deoB"
                     /locus_tag="ACG912_000442"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFQ0511789.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21736..22554
                     /locus_tag="ACG912_000443"
     CDS             21736..22554
                     /locus_tag="ACG912_000443"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0511790.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22571..23278
                     /gene="deoD"
                     /locus_tag="ACG912_000444"
     CDS             22571..23278
                     /gene="deoD"
                     /locus_tag="ACG912_000444"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFQ0511791.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23576..24262)
                     /gene="gpmA"
                     /locus_tag="ACG912_000445"
     CDS             complement(23576..24262)
                     /gene="gpmA"
                     /locus_tag="ACG912_000445"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFQ0511792.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24434..25015)
                     /locus_tag="ACG912_000446"
     CDS             complement(24434..25015)
                     /locus_tag="ACG912_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0511793.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25148..25831)
                     /gene="rpiA"
                     /locus_tag="ACG912_000447"
     CDS             complement(25148..25831)
                     /gene="rpiA"
                     /locus_tag="ACG912_000447"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFQ0511794.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26150..26563
                     /gene="rpsL"
                     /locus_tag="ACG912_000448"
     CDS             26150..26563
                     /gene="rpsL"
                     /locus_tag="ACG912_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFQ0511795.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26647..27117
                     /gene="rpsG"
                     /locus_tag="ACG912_000449"
     CDS             26647..27117
                     /gene="rpsG"
                     /locus_tag="ACG912_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFQ0511796.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27211..29298
                     /gene="fusA"
                     /locus_tag="ACG912_000450"
     CDS             27211..29298
                     /gene="fusA"
                     /locus_tag="ACG912_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFQ0511797.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29470..30657
                     /gene="tuf"
                     /locus_tag="ACG912_000451"
     CDS             29470..30657
                     /gene="tuf"
                     /locus_tag="ACG912_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFQ0511798.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31064..31372
                     /gene="rpsJ"
                     /locus_tag="ACG912_000452"
     CDS             31064..31372
                     /gene="rpsJ"
                     /locus_tag="ACG912_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFQ0511799.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31403..32032
                     /gene="rplC"
                     /locus_tag="ACG912_000453"
     CDS             31403..32032
                     /gene="rplC"
                     /locus_tag="ACG912_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFQ0511800.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32060..32683
                     /gene="rplD"
                     /locus_tag="ACG912_000454"
     CDS             32060..32683
                     /gene="rplD"
                     /locus_tag="ACG912_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFQ0511801.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32683..32973
                     /gene="rplW"
                     /locus_tag="ACG912_000455"
     CDS             32683..32973
                     /gene="rplW"
                     /locus_tag="ACG912_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFQ0511802.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33012..33845
                     /gene="rplB"
                     /locus_tag="ACG912_000456"
     CDS             33012..33845
                     /gene="rplB"
                     /locus_tag="ACG912_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFQ0511803.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33888..34166
                     /gene="rpsS"
                     /locus_tag="ACG912_000457"
     CDS             33888..34166
                     /gene="rpsS"
                     /locus_tag="ACG912_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFQ0511804.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34189..34536
                     /gene="rplV"
                     /locus_tag="ACG912_000458"
     CDS             34189..34536
                     /gene="rplV"
                     /locus_tag="ACG912_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFQ0511805.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34550..35206
                     /gene="rpsC"
                     /locus_tag="ACG912_000459"
     CDS             34550..35206
                     /gene="rpsC"
                     /locus_tag="ACG912_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFQ0511806.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35209..35643
                     /gene="rplP"
                     /locus_tag="ACG912_000460"
     CDS             35209..35643
                     /gene="rplP"
                     /locus_tag="ACG912_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFQ0511807.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35633..35821
                     /gene="rpmC"
                     /locus_tag="ACG912_000461"
     CDS             35633..35821
                     /gene="rpmC"
                     /locus_tag="ACG912_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFQ0511808.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35846..36112
                     /gene="rpsQ"
                     /locus_tag="ACG912_000462"
     CDS             35846..36112
                     /gene="rpsQ"
                     /locus_tag="ACG912_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFQ0511809.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36170..36538
                     /gene="rplN"
                     /locus_tag="ACG912_000463"
     CDS             36170..36538
                     /gene="rplN"
                     /locus_tag="ACG912_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFQ0511810.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36575..36883
                     /gene="rplX"
                     /locus_tag="ACG912_000464"
     CDS             36575..36883
                     /gene="rplX"
                     /locus_tag="ACG912_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFQ0511811.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36911..37450
                     /gene="rplE"
                     /locus_tag="ACG912_000465"
     CDS             36911..37450
                     /gene="rplE"
                     /locus_tag="ACG912_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFQ0511812.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37469..37654
                     /locus_tag="ACG912_000466"
     CDS             37469..37654
                     /locus_tag="ACG912_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFQ0511813.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37690..38088
                     /gene="rpsH"
                     /locus_tag="ACG912_000467"
     CDS             37690..38088
                     /gene="rpsH"
                     /locus_tag="ACG912_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFQ0511814.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38120..38656
                     /gene="rplF"
                     /locus_tag="ACG912_000468"
     CDS             38120..38656
                     /gene="rplF"
                     /locus_tag="ACG912_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFQ0511815.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38817..39173
                     /gene="rplR"
                     /locus_tag="ACG912_000469"
     CDS             38817..39173
                     /gene="rplR"
                     /locus_tag="ACG912_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFQ0511816.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39194..39694
                     /gene="rpsE"
                     /locus_tag="ACG912_000470"
     CDS             39194..39694
                     /gene="rpsE"
                     /locus_tag="ACG912_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFQ0511817.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39709..39888
                     /gene="rpmD"
                     /locus_tag="ACG912_000471"
     CDS             39709..39888
                     /gene="rpmD"
                     /locus_tag="ACG912_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFQ0511818.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39932..40372
                     /gene="rplO"
                     /locus_tag="ACG912_000472"
     CDS             39932..40372
                     /gene="rplO"
                     /locus_tag="ACG912_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFQ0511819.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40372..41667
                     /gene="secY"
                     /locus_tag="ACG912_000473"
     CDS             40372..41667
                     /gene="secY"
                     /locus_tag="ACG912_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFQ0511820.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41729..42376
                     /locus_tag="ACG912_000474"
     CDS             41729..42376
                     /locus_tag="ACG912_000474"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFQ0511821.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42567..42785
                     /gene="infA"
                     /locus_tag="ACG912_000475"
     CDS             42567..42785
                     /gene="infA"
                     /locus_tag="ACG912_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFQ0511822.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42818..42934
                     /gene="rpmJ"
                     /locus_tag="ACG912_000476"
     CDS             42818..42934
                     /gene="rpmJ"
                     /locus_tag="ACG912_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFQ0511823.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42952..43317
                     /gene="rpsM"
                     /locus_tag="ACG912_000477"
     CDS             42952..43317
                     /gene="rpsM"
                     /locus_tag="ACG912_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFQ0511824.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43345..43734
                     /gene="rpsK"
                     /locus_tag="ACG912_000478"
     CDS             43345..43734
                     /gene="rpsK"
                     /locus_tag="ACG912_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFQ0511825.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43814..44752
                     /locus_tag="ACG912_000479"
     CDS             43814..44752
                     /locus_tag="ACG912_000479"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFQ0511826.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44780..45160
                     /gene="rplQ"
                     /locus_tag="ACG912_000480"
     CDS             44780..45160
                     /gene="rplQ"
                     /locus_tag="ACG912_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFQ0511827.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45432..46271
                     /locus_tag="ACG912_000481"
     CDS             45432..46271
                     /locus_tag="ACG912_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0511828.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46247..47116
                     /locus_tag="ACG912_000482"
     CDS             46247..47116
                     /locus_tag="ACG912_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0511829.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47106..47903
                     /locus_tag="ACG912_000483"
     CDS             47106..47903
                     /locus_tag="ACG912_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0511830.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
BASE COUNT        15195 a         8780 c        10525 g        13548 t
ORIGIN      
        1 gtatatttag taagtagcta cacacttcta tataaattca gaagttcttc tagagtttct
       61 aaagtttgtt tcttaattag cttattgatt ctataatatt tttgatttat ataggaaatt
      121 aaaaatatta tagaatcaac actcagagaa cgggttggtt tttaatagat ttaataagta
      181 ctgataatga aatttctcct ggttgggaaa acgaagttgg ttaccctgta gaagaatttt
      241 ttcaagatta ctttgcggag tatgccgata ggataatgag agattacgta acagataaaa
      301 ctcaagaagt catcaatcaa tcccagtcat taaatgaatt atctgataag gcgaaagaag
      361 aagcagttgc cattatttct tcagggatta gtgactatat tgatatgagt ggtgaaatca
      421 attatggcga taaagacaaa attgtacgca tgataaaagc ttttatcaaa acttttgatg
      481 tgaaggcaga tcttaagttt aatgatactt acctagtagg aaaattaatt aatatccttg
      541 ggacagagaa agatacggaa acgtttattt cctctttgtc tatgttgttt acagataagc
      601 atttttcaac tcagcttggt ggagaggaac tagtggaaat cttttaacca ttacgcccag
      661 cattaataaa agtatatcaa aaaagtactc ctgatgattt ttaagattgg tttgagaaat
      721 aatgtgaaaa cactattttt aatgttaatt atagatagag atgaacgcgt catatggtta
      781 aaagaatgaa aaagtcaaag atacactgct atatttcact aaggatatag cagtgtatct
      841 ttcatgtcgt ttcactggca gttttttata aaaggaggtg tgcatttcag ttgcttttct
      901 ttggtagtta gttgaatcgt ttaagaatta acggatgtgg cagacaggtg ctctgtccaa
      961 aaaccagtaa aatctttgat ggttccatct ttatttagct gtgcctgttc aaatactgtt
     1021 ttgtttttac cgaatggatc aattgcaata attgttccga tccccgcacg tttggcagca
     1081 agtaatcctg agtaagcatc ttcgataaca agacaatcag ccggagctaa gctcagtttg
     1141 tctgccgctt taagaaaaat atccggttgc ggtttcccag ggaaagagcc gtcgtcatag
     1201 acaatcttgt cccagtcaaa ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca
     1261 ttctctttga cagtagccgt ggcaatcgtt aatggaatct gttggtcagt cagctggtca
     1321 agagtcgctt caagcccttt tgtcaactga agctcgtttt cattttgcaa gcaaagcata
     1381 cgataatagc tttctttttc ttctgacaat acggcgatct cagaggcggt caaatgctct
     1441 gaaatgaaat gtttcaaaat ctccgtattc gtccgaccat gaatattttt taaaatatcc
     1501 tcgtctgata aaccatcttt tgtatgatca tgtatcatat gcagccaagc agcttcatgc
     1561 agatgagagt ccaaaaacat tgttccgttg aaatcaaata taatcccttt catagttacg
     1621 ctcctatagt ggtattttcg gttgaatcag caatttgcag atcgacatat tgtgcatagt
     1681 gctcttgaac ctcccgcgag atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat
     1741 tataaaacac ataaaaatca tctcgattga attttttatt atcaacgagt agatatttct
     1801 tacgggaatt atttagagcg atctgctgtg cttctccttc ttcggtactg taagtagaga
     1861 tcgcttcgtt gtgaacgcca tttgaactga taaatgcttt tgtaaacttt agtcttctta
     1921 aattgtcgtt ggtgatcggt ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa
     1981 tcagtagaat ttctggtcca tctgcttgag caagaatatc aaaaactgga taattatttg
     2041 taatgatccg aattttcttg ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg
     2101 gtcctaagaa aatcgtttct ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt
     2161 ttttttcttg aatctgcaga gtggcttttt cagtgtgaga aagttcttga tctaaagtgt
     2221 aggaaagact ttgagcccct ccatggacac gcagcagctt gcccgatttt tctaattcat
     2281 ctaaatctct gcgaacggtc atatcagaaa catctaattg attgatgatt tcattaacgg
     2341 taaggattcc ttttttattg accatttcta cgatagtaag caatctttct tttttcaaca
     2401 ttgtcttcac ctctaattta aggataatct atcatataaa cggacaacag tcaatctaat
     2461 tcgaacaaaa gtaaacattt agggtggttt ttatgtttgt tacaatagat tttattgctg
     2521 ttttctggtt tttatgatta tttgtgtttg tttttgttga caaatgtttt ggatgcgttt
     2581 acaatgcaat cagttagaaa gagcgaaaac tgattataga tttgaggaga gatgtacatg
     2641 cttaaattac cagaagattt tatttttggc ggagccacag cagcctatca ggttgaagga
     2701 gccactaagg aaggtggcaa aggcgctgtt gcttgggatg actttttaga ggaacaaggg
     2761 cgattcagtc ccgatccggc aagtgatttt tatcatcaat atgcaaagga cattgaattg
     2821 tgtgaacgtt ttggtgtaaa tggtctaaga ttatccattg cttggagccg catttttcct
     2881 gatggagctg ggaaaccaaa tccagaagga atcgcttttt atcatcgtgt gttcgaagaa
     2941 tgtaagaaaa gaaacgtaac gccgtttgtg acgttgcatc attttgatac ccctaaacgt
     3001 ttgtttgacc atggagattt tttgaatcga gaaacaatag aagcatttgt ttcctatgcc
     3061 attttttgtt ttcatgaatt caaagaagtc aaggtgtgga gtacctttaa tgaaatctat
     3121 ccagttgcaa ccaatcaata tttgttaggt gttttcccac caggaatcaa atatgatttt
     3181 acaaagatcg tcgcctgtct tcataatatg atggttgctc atgcccgtgt agtgaactat
     3241 ttcaaagaaa acgaacttcc gggtgaaatt ggtgtagttc actcattaga gacgaaatat
     3301 gcggcaacag acgcgccgga agataagcat gctgcctttt tagacgatgc attgtcgatc
     3361 cgtttcttat tagatgctac ctatttaggc tattattcaa ctgaaacctt gactgctctt
     3421 gatgagatat gtgaagcaaa tcaggccagc tatcattttc ctgaagaaga tttcgttgaa
     3481 ttgaaaaaag catccacgcg taatgactac ttagggatca atcactacca atgccatttt
     3541 gtcaaagctt acgatggaga aaatgcaatc catcataacg gtacgggtga aaaagggacc
     3601 tctgtttata aagtgaaggg aatcggagaa cgtatttata aagaaggaat tcctcgaact
     3661 gattgggact ggctgattta tccagaaggt ctatatgact tgttgctgcg aattaaaagc
     3721 gattacccac attacaacaa aatctatatc actgagaacg ggatggggta taaagatcag
     3781 tttgaagatg gcattattat ggatcagccg agaatcgact atttgagagt ctatttggaa
     3841 tccttaagca aggcgataac agccggggta aatgtgaagg ggtatttttt gtggtcgctt
     3901 atggatcttt tctcatggac aaatgggtat aacaaaaggt atggcttgtt ttatgttgat
     3961 tttgaaaccc agaagcgtta cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa
     4021 accaaaacga ttatttaata agtgataatg gaggaagaaa agatgaataa agaggaatta
     4081 caaatgcttg gttttgagat cgttgcatat tcaggcgacg ctcgcagtac attattgaaa
     4141 ttattgaaag aagtaagaca aggaaacttt gaacatgttg attcggcgtt gaaagaagcc
     4201 gacgaaaatt taacccttgc acataattcg caaacaaaaa ttttagcgga agaagcatct
     4261 gggaaagaga tggagatggg attcattttt atccatgggc aggatcattt aatgaccact
     4321 ttattgctga gagatctgat tcaagacttt attgtactgt acagacagaa tcaaggatag
     4381 gagaagaacg ctatggatgg aatcattaaa caaatcgaaa aagggaaacc attttttgaa
     4441 aaaatctcac gtaatagtta tttaggagcc gttcgtgacg gatttttaac agcgatgcca
     4501 gctattttat tttcaagcat tttcattttg attgctgcgg ttcctgagat ttttggttac
     4561 caatggcctg aatctgtctc cacatggcta tggaaagtct ataattattc gatgggtgtt
     4621 gtagggttac tcgtttcagc aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc
     4681 ttaccgaaaa acaaagtaat caacaccacc tctgtgatgc tggcctctgt ggtaagtttc
     4741 ctgttgttaa gcgtctctcc gattgaaggt ggattttcaa cggaatatat ggggacaaag
     4801 ggactactgg cttcctttgt cgctgcattt ttaacagtta atttatataa gttttgtgtg
     4861 ctgaaagata ttaccattaa aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc
     4921 cgagatattt ttcctttctc tttttcagta tttgcggccg ttttaattga tttaggcatt
     4981 cgacaaatct tccaaatctc atttgcagaa gcaatcatta cggtgctgca gccgctattt
     5041 acagcagctg acgggtactt aggaatcgct attatctggg gagcgatggc gctgttttgg
     5101 tttgtcggtg ttcatggacc gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc
     5161 aatgtggaaa ccaatctgca attgttcaat tccggtgaac aagcttctaa agtactgacg
     5221 gtgggtttag ggaattttgt aggaactatg ggaggaactg gggcaacatt ggttgttcct
     5281 tttatcttcc tactctttgc taagtctaag cagttgaagg cagttgggaa agcttccttt
     5341 attccagtaa gtttcgctgt caacgaacca ttgctatttg cggcacccat gattttgaac
     5401 ccttattttt ttattccatt tttgttaaca cctgttgtaa acgtttgcct gttcaagttt
     5461 tttgtcgatg tattgggcat gaacagcttt atgtacgttt tgccttgggc aactccagca
     5521 ccgatcggtt tggttctagg aacgggcatg ggtatcttag cttttgttct agcagcggtt
     5581 ctaatcgtcg ttgacttcct gatctatcta ccattttgca aagcatacga tgctgtctta
     5641 gtagagaaag aagcacaaaa agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat
     5701 gtacgtgagg aagtacctgt ggcagcgagt caagttatca gcgatgcaaa taaagaaatc
     5761 aatgtgttgg tactttgtgc tggcgcaggg acaagtggga tgttagctaa tgctttaaaa
     5821 aaaggtgcat ctgaatcgaa agcaccgatc agtgcaatgg cgggtgctta tggttctcat
     5881 tacgaaatgc tgcaggattt tgatatggtg gtcttagcac cgcaagtcaa ttcttactat
     5941 gatgatatca aagaagacac cgatcgctta ggaatcaagc tggttgcgac aaaaggagca
     6001 gaatatatcg gattaacgag agatcctgaa aaagctgttc agtttattct gaaccaattt
     6061 caataaagga ggagccataa tgaaaatggg aatttggttg cgtaaagctg ttttatttgc
     6121 agctgtgtta ggggctttgg gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt
     6181 tattcgcgga gcagatattt cgatcctgtc agatatggaa aaatctggtg caaaatatta
     6241 tgaagacgat atttcaaaag atgccttaga gattttaaaa aataatggtg tcaactatgt
     6301 tcgactgcgt ttgtggcagg atccttataa tagtaatgga gaaagttatg gagcagggac
     6361 aaatgatctg gaaacgacga ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt
     6421 gctgctggat tttcactaca gtgatttttg ggttgatcct gggaaacaga atcttcctaa
     6481 agcatggcaa ggtctgacct ttgaagaaat gaatacggca ttgtacgact atacaaagaa
     6541 cgttctgtcg gaaatgaagc agctggacgt ttatcctgat atggttcaga tcggcaatga
     6601 actgaatagc ggaatgctct ggccttatgg caagtcttgg ggagaaggcg ggggagaatt
     6661 tgatcgattg gctgcttttt tgaagtctgg tatccaagct gttcgagaca ctcaacctaa
     6721 aacaactcca gtaatgctcc acttagctga tggtggcgat acaggagcat ttacttggtg
     6781 gtttgatgaa atcacgagtc gaggtgtgtc atttgattta attggagtgt cgtattatcc
     6841 gtattggcac ggttcaatga atgaacttca aaccaatatg aatgcgatca gccaacggta
     6901 tggtaaagaa gtgatcgtag ttgaaacagc ttatgggcat acaacagcga atgccgatac
     6961 tatgccaaat gcttttggtg aagccgaagc agctgcgggg ggatatgaac caacaccgac
     7021 aggacaagca gagtatttgc tggatcttgc cgatcggatt caggcagtac cgaataatcg
     7081 cggggcgggc ttcttctatt gggaacctct ttggtacaac ggaaatgtga gttgggcaac
     7141 acaagcaggt atgaattatc tcagtgttca atccactatg ggaaacgaat gggacaacca
     7201 agcgatgttt gattttgctg gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg
     7261 ggcgcaaata aatctagtta aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc
     7321 ttccaattgg caaacctggt ttcaacaagg aaccgctaag ggtacgatca aaacggaagc
     7381 gggcactttt tttggggact acaaactaac tttttggcag gatcaagcat acgaggcatc
     7441 agcttatcaa aatgttagtg gcataagcaa aggaacttac acattgagtg cctatgtaat
     7501 gtctggggga gagtttgata ctaatcagtt atatatcaaa aattatggtg gagcggaact
     7561 aaatactgcg gttgttaaaa caagtgaatg gacaaaaatt acaatcaaag acattccaat
     7621 tacaacaggt gtttgtgaaa ttggggttta tagcaaggct tcaggaaatg cttggacgaa
     7681 cgttgatttt gtttcattgg tgaaacaata gactgcggat cgtttcgatt caagatcgga
     7741 ggttcccttc agccacatcg ttagcgtaga aggcaggaat cgaacctgca cctcgccttt
     7801 gccaaaaata accagaaacg atttccagtc atttttacca aaacgagtaa agcaccagac
     7861 ttcatctaca ctttgtatta tgctaaagag agttcaacga tttcgtaaga agcaatgaaa
     7921 aaattgcaaa aagttgtgca gataacataa agtttgttta agaaagggta accttagaat
     7981 tccgtcgtga atatgatttg agcacttaga tggatagaaa aaagttctcg gaaaacagtt
     8041 cgtttttcga gaacttcttt ttatgataag aatgaaacaa attaacttat attatagtct
     8101 cttttggtag ggaaccatct tatttttaat gaaaatcaag cattttagct attgaagaat
     8161 gttgatttat agggattccc cctccatatt atgttggctc tttattccca ctccactgtt
     8221 gctggtggtt tagaagtgat gtcatacacg atacggttga cgtgggctac ttcgtttacg
     8281 atacgtacag agattttttg caagacttcc catgggatac gggcaaagtc agcggtcatt
     8341 ccgtcgattg aagttacagc acggataccg actgtgtagt cgtaagtacg tccgtcaccc
     8401 ataacaccga cagagcggat ccctggaaga acagtgaaat attgccagat atcacggtca
     8461 agtccagcag ctgcgatttc ttcacgcaag atagcatcag attcgcgtac gatttctagc
     8521 ttgtcttcag tgatttcgcc aagaacacga ataccaagac caggaccagg gaatggctga
     8581 cgccaaacga ttgaatcagg catgcctaac tgagtcccta attcacggac ttcgtctttg
     8641 aataatgtgt ttaatggttc gatcaattca aattgcatat cttctggcag tccacctaca
     8701 ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag attcgataac gtcagtatac
     8761 aacgttcctt gtgctaggaa agagacgcct tcttcaccag caagttttgt tgcttcgtca
     8821 tcaaaaacat atacgaattc attcccaatg attttacgtt tttgttctgg gtcagataca
     8881 cctttcaact tgtctaagaa gcgatctctt gcattgactt tgatgatgtt caagccgaat
     8941 tttccgccta gactttccat tacttgttcc gcttcgcctt tgcgtagcag accgtggtca
     9001 acaaagatag atgtcaattg gtcaccgatc gctttttgta aaagaacccc aacgacacta
     9061 gaatcaacgc cacctgaaag accaagcaag acttttttgt caccaacttg ttcacggatt
     9121 ttttctactt gcatatcgat aaagttatcc attgtccagt taccttcaca gtgacaaaca
     9181 tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg aatgacgtac ttctgcatgg
     9241 aattggatac cataaaaatt acgtgttgtg tcttggatag aagcaatcgg acaatctttg
     9301 cttgttccga ctgtttcaaa gccttcagga actttagtta ccaagtcacc atggctcatc
     9361 catactgttt gttgttttgg tgtttcttta aacaagacag cttgatcgct tgttacttct
     9421 aacaaggctt tcccatattc gcggttttta gcaggctcta cggaaccgcc aagtttgtac
     9481 gtgatcaatt gcataccata gcaaatccct aatacaggaa tacctaaatt gaagatctca
     9541 ggatcgatcc caaatgcatt ctcatcatat acactgtttg gtccgccaga aagaatgatt
     9601 ccctttggat tcatcgcttt tacttcttct gcagtaatgc ggtggctcaa taattcggaa
     9661 aagactccaa gatcacgaat acgtcttgtg atcaattggt tatactgact accgtagtct
     9721 aatacgataa tcttttcaac gttcgtcata tcggcaacgt ttgtcaaatt tattacccct
     9781 atccctttaa attttatctc aaaatagtca aactatttta aaatgctaat attttcgcat
     9841 gaaaatcgaa tcgatgatat gattttcagt tttatggaga attatatcgg ctcttcctcg
     9901 tgtaggcaag atatattcct gtaaattttt caaattcaca tttttccata catcttttgc
     9961 cattttaaag gcttcttcac gatctccgat agcaaaacga taataataat tctctggatt
    10021 ttggaaagcc gtatccagta acgcaccaaa acgatccaaa taccattttt cgatcagcac
    10081 gggatcggca tcgacataga cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt
    10141 cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag atatcaggct gctggatcaa
    10201 ttcgtacttg tctggaatga tatcatacac atcatgtgag taaacaggta cttttatctc
    10261 ttcctgacca gattttacac ggttcaagaa atcgatcagc atctccatat cataactttc
    10321 aggaaatcct ttacggtcca tgattccacg ctcttttaat atacgatttg gataaagaaa
    10381 tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg
    10441 cagcagtctg gctgttgtac tttttccaac ggctacactt ccggcgatcc cgataataaa
    10501 aggcggtact tctacgtatt tatgaagaaa taaaccttta ctcaagaaca aagattcaaa
    10561 ttctttcata taaagataaa tcagatgagc aagaggtaca tagatatctc gaacatcctg
    10621 catggaaatg cggtcattca aacttttgat attatctagt tcatcttgtg tcaaaggtgc
    10681 ttgcccatta cgataaaatt ctcgccattc ttctcttgcc acacggtagt agttcattgg
    10741 atcattcata ttagcctcct gcgttctccc atcaatggaa ctattttaac acaaaataaa
    10801 cgcaaacccc accacttaag agagattgcc gatctttttg tataatcttt ataatagaaa
    10861 gaaacggagt cttattcttg acttgcgcac ggatacataa aaggatagac tgttgtcatg
    10921 aaagaaatga gaaggatgga agacatgaca aatcattact attctgaacg cccagatacg
    10981 gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca gaacctttca atttgtaaca
    11041 gattctggtg tattttcacg tgaaacagtg gattatggtt cacgtgtcct gattgatgct
    11101 ttcgattggg aagagttacc tgatgaagga aagatcttag atgtgggatg cgggtatgga
    11161 ccgattgggt tagctttggc atttgcgaca cagcggtttg ttgaaatggt cgacattaat
    11221 gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta accaaatcga acatgttgat
    11281 atccatcaat cgaatatcta tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc
    11341 aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct
    11401 cggttgaaga agggcggtac actgacaatt gtgatccaga agaaacaagg agcgccgagt
    11461 gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa tcgtcacaaa agataaagga
    11521 tattatatca tcaaaagtgt gaaagaataa ctagctgaaa aatagtggag cgacgaaaga
    11581 gtggaactgg gcgtgcatgt acgggtacgt atcagttctt tttttgttga gaagttttta
    11641 aaaacaaaga actatttttt cttcaaaaag aaatagtagg aacagagtcg ttgtttttta
    11701 cgataaaata aataagatac agaacttgta tagaaggctg ccaagtcggt agataaagga
    11761 ggtttttatc aaaaaacata ctttcctttt ttctctattc ttttcttggg ggttttggta
    11821 cactgatagg gaatttagat agaagggagc caataggatg aagctgacca tcagagatat
    11881 cgcagaaatg gcaggcgtat cagtaacaac tgtctcacag attttgaata acaaaggaag
    11941 cagatttagt gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa
    12001 gcccgattat ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg gaatgatcgt
    12061 ccctgatgtc actgatttct tcttctccaa actgatcgaa ggtgtagaga gctatttgaa
    12121 tcctttgggc tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa
    12181 gtatttacaa gagctttccc accgctcagt ggacggaatc ttgctggcca caccaaatat
    12241 tttacctgag aaatatgcat tagacagcgg tttttatcaa aaaatgccaa tcattttaat
    12301 cgatcgcgga ttgaatcgtc gggataacgg acggcttatc gtaaaggaat atgagggcgc
    12361 ctatcaagct gtttcgctgt ttattgaaaa tggccatacg aagatcggca tgctgaagga
    12421 aaccactgga tactatcaat tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga
    12481 tcatggcctg ccttttaatg ggaaatttgt tgaacatgga gagttgactg tccaaggtgg
    12541 atatactgca tcaaaaaaat tactcaaaca taaagatatc acggctattt tttgcgggaa
    12601 tgatgccatg gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga
    12661 agatatctca gtcataggat tcgatggatt aaagctgtca gaatacatga ttccgcaatt
    12721 gacgacagtg cagcagccaa gttttgatat tggattttat gctgcacgtt tcctgatcga
    12781 tacgatcgaa tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat
    12841 tcgtgagagc gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc
    12901 atttacaaag gctagttagt gcttttttta tttttagtaa accagtttac taaagtggtt
    12961 tacctttcct aaaaataatg tataataccg aatgaagaca aggaggatca ggcaatgcga
    13021 atggtggatc tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag
    13081 tacatcgtca cgaactatac gaatggaaag attccagatt atcaagtcag tgcattattg
    13141 atggcaattt tttatcaaga catgacaaat gaagaaatca cgaacttaac attggcgatt
    13201 gctaattctg gtgatgtcat cgatttgagt tctcttgaag gaataaaggt agataagcat
    13261 tcgacaggtg gcgttggtga cacgaccaca ttgattttag cccccctagt agctagtgtc
    13321 ggagtgacag tggctaaaat gtctggacgc ggtctaggtt acacaggtgg tacactagac
    13381 aagctagaag caattcccgg atttcagatc gagctttcgg atgaagaatt tgtacgcatt
    13441 gttaatgaaa gtaaagtagc ggtaattggt caatcaggaa acttggcacc ggcggacaaa
    13501 aaattgtatg ccttgcgtga cgtcacagcg accgttgatt ctcttccgct gattgccagt
    13561 tcgattatga gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga tgtgaccact
    13621 ggagacggtg ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg
    13681 agcattggga aactggctaa ccgagaaaca gtggcggtta tctcagatat gtcagaacct
    13741 cttggcgaag ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac attacaagga
    13801 aatggtccag aagatctagt agaaatgtgc tacgccttag gaagccaaat ggttgttctt
    13861 gctggcaaag ctaagacaac cgatgaagca agaacgttgc ttcaagaagc tctggaatca
    13921 ggtaaagcgt tagcaaaatt caaagaaatg atccaaaatc aagggggaga cccagcgatc
    13981 gtggaacatc cagaacgaat tttgactgca cgctacacaa tggagcttcc tgctaaacaa
    14041 agcggagttg tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt
    14101 gctgggcgta aaaccaaaga agaagatatt gatcacgctg ttggattgaa actgcataaa
    14161 aaaattggtg acactgtgac aaaaggtgag tcactattga cgatctatag taacgacgaa
    14221 gaaatcactt ctgtcattga attattatat aaaaatattg aaattggtga atcggcaatg
    14281 aagccaacgt tgattcatga catcatcaca gaataaagga aaaaagcgga ctttaccgtt
    14341 ttaaagaaaa ggggaaaaga aatggaacta aaccgaatga tcgaccatac tattttgaaa
    14401 gcagacgcat ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt
    14461 tattctgtct gcatcaatcc agcatgggtt gcgctagcga aagaacaatt acaaggtgag
    14521 ccagtagccg tatgtacagt aatcgggttt cctctaggag caaacacacc cgagacaaaa
    14581 gcatttgaaa caacagatgc catcaataac ggtgcagatg aagtcgatat ggtcatcaat
    14641 atcggtgctt tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga agcagttgtt
    14701 gaagcagcaa aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt attagataga
    14761 gaagaaatca tccgagcttg cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca
    14821 tcaactggtt tctcaacagg cggagcaaag gtagaggatg tccgtttgat gcgtgaaact
    14881 gtaggaccag aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga agaagcaatg
    14941 gcaatgatcg aagctggtgc tacaagaatc ggaacaagcg caggagtagc aatcgtatct
    15001 ggttcaactg gtgaaggcta ttaatttttt caaaggagaa gctcatggca gaactcaaac
    15061 aagaatggat caacacagct attgaagcgt tagataaagc ctatgttcct tattcccatt
    15121 ttccggtagg cgcttgtctc gtaacggaat caggcaagat ttatcaaggc atcaacattg
    15181 agaatgcatc atttggatta acgaattgtg ccgagcgcac agcttttttc aaagcagtat
    15241 ctgaaggaga aaaaacattt acgcatctag tcgtagcggg gcacaccccc gatcctatct
    15301 ctccttgtgg tgcatgtcga caagtaatgg cagaattctg ttcccctgat atgccggtta
    15361 cgctagtagg agacaatggt gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg
    15421 catttactga aaaagatcta taactcatgt tcattcagtc agctgactgt tgaaaaataa
    15481 aacaattatt ttaggaggct ttacagaaat gaaaaaagca aaattatttg gtttaggtgc
    15541 tgtcgcacta gcagcagggt tatttttagg tgcatgcggc aacaacggct ctactgattc
    15601 atccggtggc aaaagctcat cagacacaac aactgcagca ttgatcacag acacaggtgg
    15661 tgtagacgac cgttcattca accagtcagc ttgggaagga ctagaaaaat gggggaaaga
    15721 tcaaggactt tcacgaggaa atgatggctt ccaatatttc caatcttcaa atgaatcaga
    15781 ttacatccca aatattgacc aagcattgaa tgctggattc aaaacgattt ttggtattgg
    15841 ttataaacta aaaccagcga tccaagaaca agcaacaaac aatacaggta cgaactttgt
    15901 tattatcgat gatgtcatcg atggattaga taacgttgta tctgcaactt tcaaagacaa
    15961 cgaagcttct tatcttgcag gtgttgcagc agcttatacg actgaaacaa acgtagtcgg
    16021 ctttatcggc ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg
    16081 tgttgatgca ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc aatatgctgg
    16141 tgacttctct gctccagata aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc
    16201 agatatcatc ttccatgctt caggcggtac aggtaacggt gtattccaag aagcaaaatc
    16261 attgaacgaa tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg accaatcaga
    16321 cgaaggcgaa tacacattaa acggcgaaaa gaaaaacttc acattgacat ctacactgaa
    16381 agcagttgga acagtagtag aagatttggc acaaaaatca gcggacggta aattccctgg
    16441 cggcgaacac actgtttatg gattgaaaga agacggtgtt ggtctaacag aaggacaact
    16501 ttctgacgaa gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt caggtgacgt
    16561 aaaagttcca gaaactcctg aagaaaacta aaaccatttc tatagccttg ttagacacat
    16621 gtttgacaag gctatggttt gattagaaca agaattgttc atttatccaa gctgccttgt
    16681 gacagtttgg ataagggaac aactccctga agtaatgttt gtttcaaaag aagaacgctc
    16741 actaagcgtg aatcttagtc aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa
    16801 aggtggttca agaaaatatt gtcatagaaa tgcagaatat taccaaagaa tttggaacat
    16861 tcaaggcaaa tgacaacatc aatttacaag taaaggcagg agaaattcat gcgttgcttg
    16921 gagaaaacgg tgctggcaaa tctacattga tgaacgtgct ttccggatta ttagagccga
    16981 catcagggaa aattttgatg cgtgggaaag aagtaaagat cacaagtccg accaaagcca
    17041 accaattagg gattgggatg gtccatcagc actttatgct tgttgatgcc tttactgtaa
    17101 cagaaaacat cgtgttggga agcgagccta gtcgcgcagg gatgcttgac cataaaaaag
    17161 cacgaaaaga gatccaaaaa gtttctgaac agtatggatt gtcagtcaac ccagatgctt
    17221 acgttcgtga tatttcagtt ggaatggaac aacgggtaga aattttaaaa acactttacc
    17281 gaggagcaga tgtactgatt tttgatgagc cgacagctgt attgacccct caagaaattg
    17341 atgaattaat cgtgatcatg aaggaattag tcaaagaagg caagtcgatc attttgatta
    17401 cgcataagtt agatgaaatc aaagcagtag ctgaccgctg tacagttatc cgccgtggaa
    17461 aaggaatcgg tacagtcaac gttaaagacg taacctcaca acaattagcc gatatgatgg
    17521 ttggaagaac ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt
    17581 tgtctattga aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga
    17641 acttagaggt tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga aacgggcagt
    17701 cggagttgat ccaagctttg actggtttgc gaaaggcaga aagcggacat atcaagctaa
    17761 aaggggaaga catcactaat aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg
    17821 tgccagaaga ccgtcataaa tacgggttag tcctagatat gacattatct gaaaatatcg
    17881 cactacaaac ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat tattcagtaa
    17941 tgaatgaaca tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg
    18001 ttcccgcaaa agctttatca ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg
    18061 accgagatcc tgatctgttg attgttgcaa atccaactcg tggactggat gtaggtgcga
    18121 tcgaatttat tcataaacgt ctgatcgaac aaagggacaa atacaaagca gtgttactga
    18181 tcagtttcga attagaagaa attttaaatg tttcggatcg tattgctgtt atccatgaag
    18241 gagaaattgt cggtatcgtt gatccgaaag aaacatctga aaatgaatta ggtctattga
    18301 tggcgggcta taccttagaa gaagcacgaa aagagttaga aaagggggct ggcgcacatg
    18361 aatgatcgca gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt tgttttagga
    18421 ttgatcttag gcgcgatatt gatggctgct tttggttttc agcccatcca aggttattct
    18481 tctatgctag atgcagccct tggttcgcaa agagggatag gtgaaacgtt aaggcaagca
    18541 acgccgttga tttttacagc attaggtttt tctgtagcta actcagctgg gtttttcaat
    18601 attgggcttt ctggacaagc actttgcggg tggatcgtca gtgtctgggt ggcacttgcg
    18661 tttccagatc tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg tgccttagct
    18721 ggtgcgatgg cagcggctgt tccaggttta cttcgagcat tttttggaac aagtgaagtg
    18781 atcgtaacaa tcatgatgaa ctatatctta ttatacgtaa gcacatttgc tttacatgaa
    18841 gtgatgcctg aaagctatcg ctcaagtttg gactcttcca atatgatcag tcaaaatgcc
    18901 tcactgcggt taccatggtt aacacaagct tttggcggct ctcgcgtaaa ctcaggatta
    18961 tttttagcaa tcatcgcgct tattttcgtt tggctgctga tgaagaaaac gactttagga
    19021 tttgaaatcc gttctgttgg attgaatcct tttgcttctg agtatgcagg aatgagtagt
    19081 aaacgtacga tcgttttgtc gatggtcatt tctggcgctt tggcagggct tggcggtgtg
    19141 gttgaaggat taggaacgta tcaaaacttc tttgttcaaa cgacttcgct atctattgga
    19201 tttgacggaa tggctgtttc cttgcttggt tctggatcag caatcggtat tcttttatca
    19261 gcgatcttgt tcagtatttt gaagatcggt ggacttggca tgcagacagg agcaggtgtt
    19321 ccgtttgaga tcgtcaatgt atcgattgct ttgattatct tctttgtcgg gatcaacttc
    19381 ctgattcgtt tcgtcatggc taaagtcttc caagggaaaa aacaagaaga gatcgttgcg
    19441 acaatagaat cgaaaccaac gaatcaaaac catgaagggg gcgaactata atgtcgacta
    19501 tcgaattaat tgccttgatc cttacttcta cactggttta ttcaacaccg ctaattctaa
    19561 cgtcactagg cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa
    19621 tcatggtcat gggtgctttt agttccatcg ttttcaactt gacgatgagt ccatcacttg
    19681 gttctatgac accatggatc gctattttag tgggtggcgt cgtaggtatc ctattttctc
    19741 tgattcacgc agtagcaaca gtcagtttgc gtgctgacca tattatcagc gggacagtaa
    19801 ttaatctgat ggcacctgct ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc
    19861 aaactgataa catcacgcag aatctggggt atttttcttt cccaggatta gcagatattc
    19921 cggtcattgg accaattttc tttaaaaata cattcttacc tgcttattgt gctattttga
    19981 ttgctgtcct agcttgggtg atcatcttca agacgaaatt cggcttgcgc ctccgttcag
    20041 ttggtgaaaa tcctcaagct gccgacacat taggtatcaa tgtatatgct atgcgttatt
    20101 caggcgtact gatttctggc ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga
    20161 tttccggtaa cttttcagct ggtacgatcg ttggacaagg gttcatctcc atggctgcta
    20221 tgatctttgg taagtggaat cctttagggg caatgggtgc ctcattgttc tttggttttg
    20281 ctcaaagctt gagtatcatt ggcgcacaat taccagttat ttcttcgatt ccgccagtat
    20341 tgctgcaaat tgctccatat ttgctgacaa tcattgtgct ggttgttttc ttagggaaag
    20401 cttctggtcc aaaagcaaac gggaagaact atatcaaatc aaaataatct tttagcgaat
    20461 aaaaagggtg ggttacggga agtgtttttc gtctcgccct ttttgtttta aaggcaataa
    20521 aaataaaaag aaagaaggaa taatcatgtt taaacgcgta catctgatcg tcatggattc
    20581 agtaggaatt ggagaagcac cagatgccga taagttcggt gatgttggtt ctgatacatt
    20641 aggacacatt gcgaaagaag caggattgaa cgttccacat ttagagcagc ttggtttagg
    20701 aacgatccgt cctcttgaag gtgtggaatc agtgaaagat catcaaggct atgcgacgaa
    20761 attagaagaa gtttctgtag ggaaagatac gatgacaggc cactgggaaa tcatgggatt
    20821 gaatatcaaa actccatttc gtgtgtttcc taatggattc cctgatgatc tgatcaaaca
    20881 aatcgaagaa ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt
    20941 tattgacgat tacggtgaac accaaatgaa aacaggcgat ttaattgtat atacttctgc
    21001 cgatcctgtc cttcagatcg cggcacatga agaaatcatt cctttagaag aattatatcg
    21061 gatttgccaa ttcgttcgtg atataacaaa agatgatccg tatatgattg gccgaatcat
    21121 tgcacgtccg tacgtgggtg aaccaggaga cttcacacga acaagcaatc gccatgatta
    21181 tgcgttgaat ccattcggac acacagtcct tgattctttg aaagaagcag gaaaggacgt
    21241 gattgctgtt ggaaagatca acgatatttt caacggacaa ggaatcactg aatctgtccg
    21301 cacgaaaagc aatatggatg gcgttgacca gctgctgaaa gttatgaaaa acgatttttc
    21361 aggaatcagc tttaccaatt tagtagattt tgatgcatta tacggtcatc gtcgggatgt
    21421 agtcggctat gcgcatgcga ttgaagaatt tgacttacga attccagaat tgcttgaagc
    21481 gatggaagaa gacgacttgc taatgattac agccgatcac ggaaacgatc cgacgtttac
    21541 tggtaccgat catacgcgag aatatgtacc gttattggtt tacagtaaaa aaatgaaaga
    21601 acaaggggca ttgcctcaag gatattattc agatatttca gcaacgattg ctgagaactt
    21661 tagtgtttct tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat aatcttttta
    21721 aataggagaa agcaaatgac caagttaagc gagatgttac aagaaacgac ccagtttatc
    21781 cttgataaag gagtaaaaga aattgatttt ggtttgattc taggttcagg attaggtgaa
    21841 ttagcagatg aaatcgaaga tgctgtaatg atttcttatg atgagatccc gttctttcct
    21901 gtttcgacag tagttgggca tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa
    21961 gtgttagcca tgcaagggag attccactac tatgaaggtc attccatgca aacagtgacc
    22021 tatccagtac gtgtgatggc cggactaggg gcccattcag tcattgtgac taatgcatgt
    22081 ggtggtgtca acgaatcatt tgtaccagga gatttgatgc tgattaccga tcatatcaat
    22141 tttactggtg aaaatccttt gatcggtccg aatgaagatg caatgggccc tagatttcca
    22201 gatatgagtc aagcttatac gcctgactat caagcaacag caaaagcagc agcagaaaaa
    22261 ctaggtttgg atcttaaaga aggcgtatat atgggctatt ctggaccaac ttatgaaacg
    22321 cctgcggaga tccgaatgtc tcggacaata ggagccgatg cagtagggat gtcgactgtt
    22381 ccagaagtca tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc ttgtattacc
    22441 aacttagcag caggtatgca agcgaatctg agccatgaag aagttgtaga aacaactcag
    22501 agagtcaaac aatcttttaa agcattggtt aaagaaacat tgacattatt gtaataggag
    22561 gaacaataaa atgagtgtgc atattgaagc gaaagaaggc gagatcgccg ataagatttt
    22621 acttccggga gatcctttgc gagctaaata tattgcggag acttttttgg aagatccagt
    22681 ttgttacaat caggtacgcg gtatgttagg ctttaccggc aaatataaag gagaacgtat
    22741 ttctgttcaa ggaacaggaa tgggaatgcc atcagccacc atctatgccc atgaattgat
    22801 ccagtcgtat ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga
    22861 cgtccatgtt cgtgatttag tactagcaca aggtgcagcg accagttctt cgatgatcga
    22921 aaaaaatttc caagcctttc attttcctcc aatcagtgat ttcaatctat tactgaaagc
    22981 atatgagata gctaaagaaa agggctatac ggttcatgta ggaaatgttc tttcggaaga
    23041 ttctttctac aaagatgatt tgacagagac gttccaatta gctgaacttg gcgttttagg
    23101 agtggaaatg gaagcagcgg ctctttatta cttaggcgcg aaataccatg tgcagacatt
    23161 aagtttgatg accgttagcg accatttgat caccggtgaa gaaacaacag ctgcagaacg
    23221 tcaatctacg ttcaatgaaa tgatcgaagt aggattggaa acagcgatag cagaataaga
    23281 aggaaatgaa aaagctgccc tgcattaagt aataagaaca tccaagcaaa aatagctcct
    23341 catgtttttg acttggctgg acttattatt gcaaatgtaa gcttttgaac accgtttacc
    23401 aagaggttgt gaatctgccg tttaactctg agtcacaagg aacaatttca aaaaacagct
    23461 tttcatgttt gatgaatatt gtgacttgtg accgagaagt tggcaggaga aaaccgttta
    23521 tacgaaaaaa gggactgtga caaatcgtgt cacagtccct tttttatagt gttttttata
    23581 ggtagtattt tttagcaact gttaagtcat catttaattc ataaacaagt ggttgacctg
    23641 ttggaatttc aagatccatg atgtcttcgt cagagatacc ttcgatatgt ttagctaggg
    23701 cgcgtaatga gttaccatgt gcagctacta aaacagtttt gttgtctagt aatgctggag
    23761 caatttcatc ttgccagaat ggcaaagcgc gttctaatgt cactttcaag ttttctccac
    23821 caggaatatc gcgtttgtct aacattgcgt aacgacggtc atttgctgca gaaccttcgt
    23881 cagtttcttc cattaatgga ggaagagtat cgtaagaacg acgccagatg tgcacttgtt
    23941 cgtcgccgta tttttcagct gtttcttttt tgttcaaacc ttgtaattta ccatagtgac
    24001 gttcgtttaa gcgccatgat ttgatttgtg gtacccaaag ttgatctgaa tattctaaga
    24061 tcaagttaca tgttttgatt gcacgagtta aaacagaagt gtaagcaaca tcaaattcga
    24121 ttccagcttc tttgattttg cgtccgcctt cttttgcttc ttcgattcct tctggtgcta
    24181 gatttacatc agcccaacca gtaaattggt tcaatgcatt ccattcacta agaccgtgac
    24241 gagaaaaaac taattttggc atgtttagct ctccttttaa gataggttta tatttctgaa
    24301 attttcagtt ctgttttatt gtacaatttt ttggaagaga tttccatcga aaaattagat
    24361 aagattctat gagaaaacaa gtagagaata taaaaaaacg aacagtgccc acgcaactgt
    24421 tcgtttttag gtattagatg cataattgct cgatttcttt ttcaggtaaa ttgatatatt
    24481 tataacatgt tccaactgaa acaccgcatt ttgaagaaat aaattggatc gtttcttttt
    24541 tttcataata gagacgtcgg atttttttga tcgtttttgc atcgattttt ggtcttccgc
    24601 cgatttttcc ttttttacga gcttcatcta atccaactaa tgtacgttct ttgatcaatg
    24661 cgcactccat gtttgctagg ttttccatta attggaaata gatttttccc atttcgcgcg
    24721 tatcgattcc ttcatcaaga ctaatcaagt gcagcccgcg atctttgaat aattgcgtga
    24781 gttctgttaa ttgccgcgta gatttgccta atcgatgaag ttggcaaatg actagtgcat
    24841 cgccttgttg catgtcagcg atcactgact ccaaatgaac agcagtagaa tcattttgtt
    24901 ccacttcata tgtctcatgg aaaatttctt gacaaccaaa gtcagataat tgatcaacct
    24961 gagttgttaa atcaacgtca tttatggttg tgcgtgcata accaataatt ttcatggtga
    25021 ttcctttcta gcagtgattt tctaactgga tataacagta aaaatgtagc ataaagtacg
    25081 tcaaaaagat agttttttcg caaattttcc gctgtctttt tttattggat gatcaagcag
    25141 tttttcttta tttacgacgg attgcgtcaa gaatctcaac gcctttttct gagccgacaa
    25201 ctactttaga aaccatgttt aggaaaagcc catgttctac taccccaaca agttcatcta
    25261 aataggtagc taagctttct ggctgttcga tgacttctag atgcagatca atgatgtaat
    25321 gaccaccatc tgtggttaag acttctccgt tttcgtctgt acgcaagacg ggttggaatc
    25381 ttttttcttc aaaaatatga agtagctgtt gggagccata agggatcact tcaactggga
    25441 gtgggaactt cccaagttta tcgactaatt tagaagaatc aacgatccaa atcgtttctt
    25501 ttgagtacgt agcgactatt ttctcgaaaa gcaaagcagc gccgccgcct ttgatccctt
    25561 gaaaatcagc actgatttca tcagcaccgt ctattgtcaa atcgactacg gggacttcat
    25621 caatactctt taacggtatt cctaattttt ccgcttgttc ttttgtcgca tttgaagtag
    25681 taactcctgt gatacgtaaa ccttcttcac gcatacggcg accaatctct tctaccatat
    25741 aataggcggt tgaaccggtc ccgagaccta cgatcatccc gtcctttacg aatttcgcgg
    25801 cttcaatgcc agctaattgt tttaaattca tctttgcgtc cacctttttt tagtttgagc
    25861 atcgtgcttt cctcactatt ttagcgtgat ttacggtaaa aagataccat tcttttttat
    25921 tccaataaag ctttttcaaa aaaaattgca tctcttgttg acatgttttt tcattcgtga
    25981 tattctaaca aaggtgcttt atctacaggt ttctgtgaag aaacgtgctg actgtttatc
    26041 aaagcatatt tgataaggat gcagacattg catttatttt ttatcacaaa aaaagaacca
    26101 cctggatgtg tggaactaga cgcaaatcgt gggaaggagg aaacaaggaa tgcctacaat
    26161 taaccaatta gtacgtaaac ctcgtaaatc aaaggtggaa aaatctaatt cacctgcttt
    26221 gaacaaagga tataacagct ttaagaaaac tcaaactaac gtaaactcac ctcaaaaacg
    26281 tggggtagca actcgtgtgg gtacaatgac acctaaaaaa ccgaactcag ctttacgtaa
    26341 atatgcacgt gttcgtttgt caaacttaat tgaagttact gcttatatcc cagggattgg
    26401 tcataactta caagaacaca gtgttgtatt attacgcggt ggtcgtgtaa aagacttacc
    26461 aggggtacgt taccatatcg ttcgcggtgc tttagatact gctggtgtta acgaccgtaa
    26521 acaaagccgt tctaaatacg gaactaaaaa acctaaagct taattttaat tattaaacaa
    26581 atgaaacttg cttctactat atagaagtaa ttactatgaa caatatttcg gaaggaggag
    26641 ttacggatgc cacgtaaagg tcctgttgca aaacgtgatg ttttaccaga tcctatttat
    26701 aactcaaaat tagtaactcg cttgatcaac cgtgtaatgg ttgacggaaa acgcgggatt
    26761 gcagctaaca ttatctataa tgcctttgac ttgatcaaag aatctacagg taacgatcca
    26821 ttagaagtgt ttgaacaagc aatgaaaaac gttcaacctg ttcttgaagt taaagcacgt
    26881 cgtgttgggg gttcaaacta ccaagtacca gttgaagttc gccctgaacg ccgcgttact
    26941 ctaggtcttc gttgggtagt aaactacgct cgcttgcgcg gtgaacacac aatggaagaa
    27001 cgcttagcaa aagaaatcat ggatgctgca aacaacacag gtgcttctgt taaaaaacgt
    27061 gaagacactc ataaaatggc tgatgccaac cgtgcgtttg ctcactatcg ctggtaagat
    27121 tctctctgtt tggtcggctt ttataagtcg acctcagata gtgtccttat ttttagcaac
    27181 tagatagtaa agagaggaga aacaaggaga atgaccagag aattttcttt acaaaacact
    27241 cgtaatatcg gtatcatggc ccacatcgat gctggtaaaa cgactgctac tgaacgtatc
    27301 ttgtactata ctggtaaaat ccataaaatt ggtgaaactc atgaaggagc ttcacaaatg
    27361 gactggatgg aacaagaaca agaacgtggt attacgatca cttctgctgc tactacagca
    27421 caatggaagg gtcaccgaat caacatcatc gacacaccag gacacgtaga cttcacagtt
    27481 gaagttgaac gttcattgcg tgtacttgat ggtgctgtaa ctgttcttga tgcacaatca
    27541 ggtgttgaac ctcaaactga aacagtttgg cgtcaagcaa caacttatgg tgtacctcgt
    27601 atcgtatttg caaacaaaat ggacaagatc ggtgctgact tcttatattc agtatcaact
    27661 ttacatgacc gtttgcaagc aaacgctcac ccaatccaat taccaattgg tgcggaagac
    27721 gacttcacag gaattatcga ccttgtaaca atgaaggctg aaatgtatac aaacgatctt
    27781 ggaacagaga tcgaagaaac agaaattcct gaagaatacc gcgaattagc tgaagaatgg
    27841 cgcgaaaaat tggttgaagc tgtagctgaa actgatgaag aacttacttt gaaatactta
    27901 gaaggtgaag aaatcactga agctgagtta aaagaaggta tccgccgcgc aacagttaac
    27961 gtagaattct acccagtact atgtggttca gcatttaaga acaaaggggt tcaattacta
    28021 ttggacgctg ttcttgatta cttgccatca ccgttagata tcccagctat caaagggatt
    28081 gaccctaaaa ctgatgaaga agtagaacgt cctgcagacg acagcgctcc tttctcagct
    28141 ttagcattta aagttatgac tgacccattc gtaggtcgtt taactttctt ccgtgtgtac
    28201 tctggtgttt tacaatcagg ttcatacgta caaaacgcaa caaaaggcaa acgtgaacgt
    28261 gttggtcgta tcctacagat gcatgccaac tctcgttcag aaatctctga agtttacgct
    28321 ggagatatcg ctgctgctgt tggtttgaaa gacacaacta ctggggacac actatgtgat
    28381 gaaaagagtc tagttattct tgaatccatg gaattcccag aaccagttat ccaagtcgct
    28441 attgaaccta aatcaaaagc tgaccaagat aaaatgggtg tggctttaca aaaactatct
    28501 gaagaagatc ctactttccg tgctgaaaca aacgtggaaa caggcgaaac aatcatcgct
    28561 ggtatgggtg aacttcactt agatatcatc gttgaccgta tgcgtcgtga attcaaagtt
    28621 gaagctaacg taggtgcgcc tcaagtatct taccgtgaaa ctttccgtgc tggcactcaa
    28681 gccgaaggta agtttgtacg tcagtctggt ggtaaaggtc aatacggtca cgtatggatt
    28741 gaatttacac caaatgaaga aggtgctggt ttcgaatttg aaaatgcgat cgtcggtggg
    28801 gttgttcctc gtgaatacat tccagctgtt gaaactggtt tgaaagatgc tatggaaaat
    28861 ggtgtcttag ctggctatcc attagtagat atcaaagcta aactttacga tggttcatac
    28921 catgatgtcg attcaaatga aactgccttc cgtgttgctg cttctatggc attacgtgca
    28981 gcagctaaga aagctaatcc agtaatcctt gaaccaatca tggctgtaga agttgttatc
    29041 cctgaagatt atttaggtga tgttatgggt cacgtaactg ctcgtcgtgg acgtgttgaa
    29101 ggtatggaag ctcgcgctgg cggccaacaa gttgttcgtg cgatggttcc acttgctgaa
    29161 atgtttggtt atgctacaac tcttcgttct gctactcaag gtcgtggtac atttacaatg
    29221 acatttgacc actacgaaga tgtaccaaaa tcagtacaag aagaaatcat taagaaaaac
    29281 ggcggaaaag ctgaataatt tcttgaaacc ggatagtcct gcaaagagat tatccaaaga
    29341 gactatattt ttaatatagt ttcgtgtaaa ataattatcg atgatatggg catgtggtgt
    29401 accttgtgta catcatatac ctatcagaca aataaaaaat atattgtatt tttaggagga
    29461 cattttaaaa tggcaaaaga aaaatttgac cgttctaaac cacacgttaa cattggtact
    29521 atcggacacg ttgaccatgg taaaactaca ttaacagctg caatcacaac tgtactatct
    29581 aagaaaaacg gcggccaagc tatggcttac gatcaaatcg atggtgctcc agaagaacgc
    29641 gaacgcggaa tcacaatctc aacagctcac gttgagtatg aaacagatac acgccactac
    29701 gctcacgttg actgcccagg acacgcggat tacgttaaaa acatgatcac tggtgctgct
    29761 caaatggacg gagctatctt ggtagtttct gctgctgacg gcccaatgcc tcaaactcgt
    29821 gaacacatcc tattgtctcg tcaagttggt gttccttaca tcgttgtatt cttgaacaaa
    29881 gtagacatgg ttgatgacga agaattacta gaattagttg aaatggaagt tcgtgaccta
    29941 ttaacagaat acgaattccc tggtgacgat gttcctgtag ttgctggatc agctttgaaa
    30001 gctctagaag gcgacgcttc atacgaagaa aaaattcttg aattaatggc tgcagttgac
    30061 gaatacatcc caactccaga acgtgacaac gacaaaccat tcatgatgcc agttgaagac
    30121 gtgttctcaa ttactggacg tggtactgtt gctacaggtc gtgttgaacg tggacaagtt
    30181 cgcgttggtg acgaagttga agttgttggt attgctgaag aaacttcaaa aacaacagtt
    30241 actggtgttg aaatgttccg taaattgtta gactacgctg aagctggaga caacattggt
    30301 gctttactac gtggtgttgc acgtgaagac atccaacgtg gacaagtttt agctaaacca
    30361 ggtacaatca cacctcatac aaaattctct gcagaagtat acgtgttgac aaaagaagaa
    30421 ggtggacgtc atactccatt cttcactaac taccgtccac aattctactt ccgtacaact
    30481 gacgtaacag gtgttgttga attaccagaa ggaactgaaa tggttatgcc tggcgacaac
    30541 gtaacaatgg aagttgaatt aatccaccca atcgctatcg aaaacggtac taaattctca
    30601 atccgtgaag gcggacgtac agttggtgcc ggtgtcgtta cagaaatcaa agcttaattc
    30661 acttaagttt taaagtaaag caacatttat tcggacgtaa tcgctcggaa cgaaggtctt
    30721 aggatctttg ttccgagctt ttttatttat tgcaggatca ggaaaaaaga caggagtatt
    30781 ataaatagaa gaaacacgaa ataaaagagt cagtattttc taaatgaaac tttatgaaaa
    30841 caaaatgttt tttacaagaa aaagccttgc tttgtgagtt tatatatatt ataatgacaa
    30901 aggtgcgttt actttagtga attgcacaga cgtaatcaga atgatttcgt cggctaagaa
    30961 acgagaggtt gcgacacgcc cggacgcttt gccacgaacg agcgtgacgg aaattttcgt
    31021 ggagctatgt ctacttttca aaataggcga aggagggaac aagatggcaa aacaaaaaat
    31081 tcgtatccgt ttaaaagcgt atgaacaccg tattttagat caatcagcgg ataaaatcgt
    31141 ggaaactgca aaaagaactg gagctagcgt gtctggtcca attccattac caactgaacg
    31201 cagtctttac acaatcatcc gtgcgactca taaatacaaa gattcacgcg aacaattcga
    31261 aatgcgtaca cacaaacgtc ttatcgacat tgtgaaccca acaccaaaaa ctgttgatgc
    31321 tttgatgaag ctagacttac catctggtgt aaacatcgaa atcaaattat aaaactaaaa
    31381 caaaaataat ggaggtgtac tcatgaccaa aggaatctta gggaaaaaag tgggaatgac
    31441 acaaatcttc actgaatctg gtgaattaat tccagtaact gttgtagaag ctactccaaa
    31501 cgtagtacta caagtgaaaa caatggaaac ggatggctac gaagctattc aagttggtta
    31561 ccaagatatg cgtgaagttt tatcaaacaa acctgcgaaa ggtcatgttg caaaagcaaa
    31621 cacggctcct aagcgcttca ttcgtgaatt caaaaatgtt gagctaggag aatatgaagt
    31681 aggaaaagaa attacagtag atgttttcca agcaggagac attattgatg taacaggtac
    31741 tacgaaaggt aaaggattcc aaggggttat caaacgccac ggacaaagcc gcggaccaat
    31801 ggctcacggt tctcgttacc atcgtcgtcc tgggtcaatg ggtccagttg cacctaaccg
    31861 tgtatttaaa aacaaacgtc ttgctggccg tatgggtggc aaccgcgtaa ctatccaaaa
    31921 cttggaagtt gtacgtgtcg atgcagaaag aaatgttatc ttgatcaaag gtaatgttcc
    31981 tggagctaaa aaatctttaa ttactatcaa atcagctgtg aaagctaaat aattgtagga
    32041 agaaaggagg aactaaggaa tgccgaatgt agcattattc aaacaagatg gaagccaaaa
    32101 cggtgaaatc acacttaacg aagaaatctt cggaattgaa cctaatgaaa gtgttgttta
    32161 cgatgcaatc gtaatgcaac gtgcgtcatt aagacaagga acacacgcgg ttaaaaaccg
    32221 tagcgctgtt cgcggcggcg gacgtaaacc atggcgtcaa aaaggaacag gtcgtgcacg
    32281 tcaaggatct atccgctcac cacaatggcg tggaggtggc gtagtctttg gaccaacacc
    32341 acgttcttac agctacaaac ttcctaaaaa agttcgtcgt ttagcaatga aatctgtatt
    32401 gtcagaaaaa gttgctgaaa acaacttggt agcaatcgaa ggcttgaact tcgacgcacc
    32461 aaaaacaaaa gaattcaaac aagttcttgc taacttatct atcgactcta aagtattggt
    32521 tgttctagaa aaaggtaacg actttgctgc tttatcagca cgtaacttac caaacgtttc
    32581 tgtagtaact tctgataatg tgagtgtgct agacgttgta tcaaatacaa aagtgttagc
    32641 aacacaaact gctcttactc aaattgagga ggtgcttgca taatgaactt actagacgta
    32701 atcaaacgcc cagtgatcac tgaaaaatca atgcttgcca tggatgacaa gaaatacact
    32761 tttgaagtgg acactcgcgc aaacaaaact ttagtaaaac aagcggtaga agctgctttt
    32821 gacgttaaag tcaaaaacgt gaacatcgtg aacgtacgtg cgaaattcaa acgtatgggc
    32881 aaatatgcag gatatacaaa aaaacgtcgc aaagcgatcg ttacattgac tgaagattca
    32941 aaagaaatcc aattattcga agctgctgaa taagcactaa tagataactc attaaattag
    33001 gagggaaaaa cgtggcgatt aaaaagtaca aacctacctc aaatggtcgt cgtaacatga
    33061 caggttctga ttttgctgaa atcacaacat caacacctga aaaatcattg ttacaaccat
    33121 tgaaaaacca tgctggacgt aacaacaacg gccgcatcac tgtacgtcat caaggtggcg
    33181 gacacaaacg ccaatatcgc gtgatcgact tcaaacgtaa caaagataac gttgttgcaa
    33241 ctgttcaaac gatcgaatat gatccaaacc gttcagctaa catcgcgtta gtacactatg
    33301 aagatggagt gaaagcttac atcttagcac ctaaaggact agaagtgggt atgaaacttg
    33361 tttcaggtcc agaagcagat atcaaagtcg gaaatgctct tccattagaa aacattccag
    33421 ttggtactgt tatccacaac attgaaatga aacctggtaa aggtggacaa ttgatccgtt
    33481 ctgctggtac aagtgctcaa gtacttggta aagaaggcaa atacgtattg atccgcttga
    33541 actctggtga agttcgtatg atcttagctg catgccgtgc tacaatcggt tctgtaggta
    33601 acgaacaaca cgaattaatc aacatcggta aagctggacg ctctcgttgg atgcgtaaac
    33661 gcccaactgt acgtggtagc gtaatgaacc ctaatgatca cccacacggt ggtggtgaag
    33721 gtaaagctcc tatcggacgt aaagcaccag tttctccttg gggtcaacca gctcttggtt
    33781 acaaaactcg taacaaaaaa gctaaatcag acaaacttat tgttcgtcgt cgcaaaaata
    33841 aataattact ttgaccatgt gtcgcacatt ttgagaggag gttcaccatg ggtcgtagtt
    33901 taaagaaagg accttttgtc gatgagcatt taatgaaaaa agttgaagct caagcagggg
    33961 ttgaaaagaa aaaagttatc aaaacttggt cacgccgttc aacagttttc ccaagttttg
    34021 ttggatatac catcgcagtt tatgatggac gtaaacatgt accagtttac atccaagaag
    34081 acatggtagg acacaaatta ggtgaatttg cacctacaag aacttatcgt ggacatgctg
    34141 ctgacgataa gaaaacgaaa cgctaatttg aggggaggat aaatccaaat ggcagaacaa
    34201 atcacatcag ctaaagcaac tgcaaaaaca gttcgcactt cacctcgaaa agcccgttta
    34261 gtaattgacc ttatcagagg taaaagtgtc gcagatgcaa tttcaatctt gaaattcaca
    34321 ccaaacaaat ctgctggaat cattgaaaaa gtattgatgt cagcagttgc gaatgcagaa
    34381 aataactttg acttagatgt tgaaaacttg gtagtaagcg aagcttttgt taacgaagga
    34441 cctacaatga aacgtttccg tcctcgtgca aaaggatcag cttctccaat caacaaacgt
    34501 acaagtcata tcacggtagt cgtatcagaa aaataaggag ggataatcag tgggtcaaaa
    34561 agtacatcca attggaatgc gtgtaggcat catccgcgac tgggatgcta aatggtatgc
    34621 tgaaaaagag tatgctgagt tcttacacga agatttaaga atccgtaaat ttatttcaac
    34681 taaacttgct gatgctgctg tatcaacagt tgaaatcgaa cgcgcaacag gtcgcgtaaa
    34741 catttcaatt cacacagcta aaccaggtat ggttattggt aaaggcggtt ctgaagtcga
    34801 aagcctaaga aaagaattaa acaaattaac tggtaaaaga atccatatca atatcgtgga
    34861 aatcaaaaaa ccagatctag atgcaaaatt agtaggtgaa ggaatcgcac gtcaattaga
    34921 aaaccgtgtt gctttccgtc gcgctcaaaa acaagccatc caacgctcaa tgcgtgctgg
    34981 cgctcaaggg atcaaaactc aagtatctgg tcgtctaaac ggagcagata tcgctcgttc
    35041 agaaggatac tcagaaggaa ctgttcctct tcatacttta cgtgcggaca ttgattacgc
    35101 atgggaagaa gcagatacaa catacggaaa actaggagtt aaagtgtgga tctatcgtgg
    35161 agaaattctt ccaactaaaa aaaacactga gaaaggaggg aaataatcat gttagtacct
    35221 aaacgtgtaa aacaccgtcg tgaattccgc ggaaaaatgc gcggggaagc taaaggcgga
    35281 aaagaagtag cattcggtga atacggtttg caagctgttg attcacattg gatcacaaac
    35341 cgccaaatcg aagctgctcg tatcgcaatg actcgttaca tgaaacgtgg tgggaaagta
    35401 tggattaaaa ttttccctca caaatcttat actgccaaag caattggggt acgtatgggt
    35461 tctggtaaag gggcacctga aggatgggtt gcaccagtaa aacgtggtaa aatcatgttt
    35521 gaaatcgcag gcgttcctga agaagtagct cgtgaagcgt tacgtctagc ttctcacaaa
    35581 ttaccaatga aaactaagat cgtaaaacgt gaggaaatgg gtggtgaatc gaatgaaggt
    35641 taaagaaatc agagaattaa ccactgccga aatgcttgat caagaaaaac aattaaaaga
    35701 agaattgttt aatcttagat tccaattagc aacaggtcaa ttagaaaaca ctgcacgtat
    35761 taaagaagta cgtaaatcga ttgcacgcat caaaactgtg ttgcgtgaac aagctaagta
    35821 attatggaag gaggccatta agcgtatgac tgaaggaaga aatcaacgca aagtttatca
    35881 aggtcgcgtg gtttcagaca aaatggataa aacaattacc gttgctgtcg aaacaaagaa
    35941 aaaccaccct atctatggca aacgcatgaa ctattctaag aaatacaaag tgcatgatga
    36001 aaacaacaca gcaaaagttg gcgacatcgt gagaattatg gaaactcgtc cattatcagc
    36061 tacaaaacgt ttccgtttgt tggaagtagt cgaagaagca gttattatct aataacacga
    36121 gattttccta tatagattga atgcaaaatc tgaaaggagg atacacaacg tgatccaagc
    36181 agaaagtcgt ttaaaagtcg ctgataactc aggcgcacgt gaaattttga cgatcaaagt
    36241 ccttggtgga tctggtcgta aaactgctaa catcggtgat gtgatcgttg ctacggttaa
    36301 acaagcaacg ccaggtgggg ttgtcaaaaa aggtgacgtc gttaaagccg ttatcgttcg
    36361 aacaaaatca ggagctcgtc gtacagacgg ttcatatatt aaatttgatg aaaatgctgc
    36421 agtgattatt cgtgatgata aaagccctcg tggaacacgt atctttgggc cagttgcacg
    36481 tgaactacgt gaaaacaact tcatgaagat cgtttcacta gcaccagaag ttttataatc
    36541 ttgagacact tatcaaagga ggtgcgaaac agttatgttt gttaaaaaag gcgataaagt
    36601 taaagttatc actggaaaag acaaaaacaa agaaggcgtt gtactagcag cgttcccgaa
    36661 aaaagataaa gttattgtcg aaggtgtaaa tgtcgttaaa aaacaccaaa aaccttctca
    36721 agctgctcct caaggtggaa ttgttgagat ggaagcgcca attcatgttt ctaacgtgat
    36781 ggtggttgac tctactggtg tagctggccg agttggctac aaagaagtag acggcaaaaa
    36841 agtccgtgtt tctaaaaaaa ccggtgaagt tttagataaa taaaagttta aggaaggagg
    36901 gtcttactga atgaaccgcc taaaagaaaa atatcttaaa gaagtaactc catcattgat
    36961 ggaaaaattt aactatagct ctgttatgca aacacctaaa gttgaaaaaa tcgtcatcaa
    37021 catgggtgtt ggtgatgcgg tatcaaacgc taaaaactta gataaagcag ttgaagaatt
    37081 aactttgatc actggtcaaa aaccaatgat tacaaaagct aaaaaatcaa tcgctggatt
    37141 ccgtttacgt gaaggaatgc caattggtgc aaaagttaca ttgcgcggag aaagaatgta
    37201 cgaattctta gacaaattgg tttctgtttc actaccacgt gtacgtgact tccatggtgt
    37261 aagtaaaaaa gcctttgacg gtcgtggaaa ctatacttta ggtattaaag aacaattgat
    37321 cttcccagaa gtagattatg atttagtaga taaagtacgt ggtatggaca tcgttattgt
    37381 aacaacagca aactctgatg aagaatctcg tgagttgttg acacaattag gcatgccatt
    37441 ccaaaaataa ttaaaggagg cgaactacgt ggctaaaaaa tcaatgattg ctaaaaacaa
    37501 acgtcctgca aaattttcaa cacaagctta tactcgttgc gaacgttgcg gacgtccaca
    37561 ttcagtttat cgtaagttcc atctttgccg tatttgcttc cgcgaacttg cctataaagg
    37621 tcaaattccc ggcgtgaaga aagctagctg gtaaaaagta aattcgcata agaaggaggt
    37681 aaaagatcaa tggtcatgac agatccaatt gcagattttc taactcgtat ccgtaacgca
    37741 aacatggtta aacatgagtc tttagaagta cctgcatcaa aaattaaacg tgacatcgct
    37801 gaaatcttga aacgtgaagg tttcgttcgt gatgttgaat acatcgaaga tgacaaacaa
    37861 ggcgtgatcc gtgttttcct aaaatttggt aaaaacggcg aacgtgttat cacaaactta
    37921 aaacgtatct ctaaaccagg tttgcgtgct tatgtcaaag ctgacgaagt accaaaagta
    37981 ttgaacggtt taggtatcgc aatcatttcg acttctgaag gtgttgtcac tgataaagaa
    38041 gctcgtgaaa aaaatatcgg cggcgaagtt atcgcttacg tatggtaatc aagtagaatc
    38101 tacaaggagg tgtctttaag tgagccgtat cggaaataaa gtcgttgtta tccctgaagg
    38161 agtaacagtt actcaagacg gaaacaacgt tacagttaaa ggaccaaaag gtgaattgac
    38221 tcgtactttt tcagcagata tcaaaatgaa tatcgaagga aacgaagtaa ctttcactcg
    38281 tccaaacgac agtaaagaaa tgaaaacaat tcatggaaca actcgtgcga acttcaacaa
    38341 catggttgta ggtgtcagcg aaggtttcca aaaagcgtta gaactaatcg gggttgggta
    38401 ccgtgcacaa ttacaaggat caaaacttgt attgaacgtt ggttactcac atccagtaga
    38461 aatcacacca ccagctggag taactgttga agtaccagct aacacacaag taattgttaa
    38521 aggtgcgaac aaagaagaag ttggcgaatt agctgcaaac attcgtggaa ctcgtcctcc
    38581 agaaccttat aaaggtaaag gtattcgtta tgttggcgaa tatgtacgcc gtaaagaagg
    38641 taaaactggt aaataatcgt ttagaaaaat tgcatagaag ccagcagcag gaaaaaggca
    38701 tttggctgaa gtttaccttc aaaaaaatac atttttactt gtgccgcagc ttctattttt
    38761 cgaaacgtat ttactgcttg aaatagtagt acaataaaaa taaagaggtg acaattgtga
    38821 ttacaaaacc agataagaac aaaacacgtc aaaagagaca tcgtcgtgta cgtaacaata
    38881 tctctggtac tgctgagcgc ccacgcttga acgtttttcg ttctaacaaa aacatctacg
    38941 ctcaaattat tgatgacgta gcgggtgtaa cgctagcaag tgcctctacc ttggataaag
    39001 aaatttcagg tggaacaaaa acagaagcag cacaagctgt cggtaaacta gttgctgaac
    39061 gtgcagctga aaaaggcatt aaagtagtag tctttgaccg tggtggatac ctttaccatg
    39121 gccgtgtgca agctttagct gaagctgctc gcgaaaatgg actagaattt tagaagaagg
    39181 aggaatacca ttcatggttt atatcgatcc aaaacatttg gaattagaag accgcgttgt
    39241 tgcaattaac cgtgtaacaa aagttgttaa aggtggacgt cgtctacgtt ttgctgcttt
    39301 agttgttgtc ggtgacaaaa acggacacgt aggatttggt acaggtaaag ctcaagaagt
    39361 tcctgaagca atccgtaaag caattgaaga tgcgaagaaa aacttagttg aagtacctat
    39421 ggttggttca acaatcccac acgaagtaat cggtgctttt agtggtggta gaatcctaat
    39481 gaaacctgct gtagaaggtt ctggggttgc tgctggtgga ccagttcgtg ccgtattaga
    39541 attagctggg gtagcagata tcacttctaa atctttaggc tcaaatacac caatcaacgt
    39601 tgttcgcgca acagttgaag gtttgaaaca attgaaacgt gcagaagaag ttgcagaact
    39661 tcgcggcaaa tcagttgaag aattaatcgg ataaggagga caacaataat ggctgaatta
    39721 aaagttactt taaaacgcag tattatcgga cgtcctcaaa accaacgcga tacagttaaa
    39781 gcgttaggtc ttacaaagat taacagttca gtggttaagc cttctaatga agcaattaaa
    39841 ggcatgatca atactgtttc tcacctagtg gacgttgaag aaatttaatt tagaaaaatc
    39901 ataactagaa cattaaggag gtgccaaacc tatgaaactt catgaattaa aacctgctga
    39961 aggttcacgc caagtacgta accgtgttgg tcgtggtact tcatctggta acggaaaaac
    40021 tgccggacgc ggacaaaaag gacaaaaagc tcgttcaggc ggcggtgtgc gtttaggatt
    40081 tgaaggggga caaacaccat tgttccgtcg tttaccaaaa cgaggattca caaacgtgaa
    40141 ccgtaaagac tatgcagtca ttaatcttga tgtattgaac cgctttgaag acggagctga
    40201 agtaactcct gttgctttag ttgaagctgg aatcgtgaaa aacgaaaaag ctggaatcaa
    40261 agtattagct aatggggaac taaacaagaa attaactgtg aaagcagcaa aattctctaa
    40321 agcagctcaa gaagcaattg aagcggctgg tggatcaatc gaggtgatct aatgtttaga
    40381 ctattaaaag atgcttttaa agtaaaagac attagatcga aaatcctatt tactgtattt
    40441 gtgttgtttg tatttcgcct aggtgcacat attactgtac caggcgtaaa tgctgcttca
    40501 ttgcaaaacc tttcttcttt gccatttttc aacatgttga acttggtaag tggtagtgcg
    40561 atgcaaaact tttccatttt ctcaatgggg gtttcaccat acattactgc ttctatcgtc
    40621 attcagcttc ttcaaatgga catcgtgcct cgatttgttg aatggtcgaa acaaggggaa
    40681 gttggtcgta aaaaactgaa tcaagcgact cgatatctga cattagtatt ggcatttatc
    40741 caatctgtcg gtatcactgc tggattcaat caatacgctc aattaggttt cgtgaataat
    40801 cctaacgtag caacttacgt aacgatcggt ttgattctaa cagccggtac gatgtttgtt
    40861 acttggctag gagaacagat cacagaaaaa ggtatcggaa atggcgtatc aatgatcatt
    40921 tttgcaggta tcatttctag attgcctgaa agtatcagag aaatttatga agattacttt
    40981 ataaatattg atccttcaga catatggaaa tctgctattt ttgtggccat tttactagta
    41041 gcggtcttgt tgatcgtgac attcgtaaca tttttccaac aagctgaacg caagatcccg
    41101 atccaatata cgaaacgcgt agctggcgcg cctacaagca gctatctgcc gttgaaagta
    41161 aatgcagctg gagttattcc agttatcttt gctagttcgt ttattgctac gccaaatgct
    41221 gttttacaag cattgagcgg tagattcggt tctgaaaact ggtacaaagt gatgatggag
    41281 atctttaact ataatacagc acctggtgct acgatttata cgattctgat cgttgccttc
    41341 actttcttct acgcctttgt tcaagtaaac cctgagaaat tagcggaaaa cttgcaaaag
    41401 caaggaagtt atataccaag cgtacgccct ggtaagggga ctgaagagta tgtctcaaga
    41461 ttactgatgc gtttaagtac agttggtgca gtcttccttg gtttagtggc attgcttcca
    41521 atcattgctc aaatggtttg ggatcttcca caatcaatcg gattaggcgg tacaagctta
    41581 ctgatcgtta tcggtgtagc tttagaaact gcgaaacagt tagaaggctt aatgctgaaa
    41641 cgtaaatatg ttggattcat tgagtaagaa agcgtgttga ggtttcccct caacacacgt
    41701 tctcacacat ttattaggag ggaaaacaat gaacctcatt ttaatggggc tgccaggcgc
    41761 aggtaaagga acacaagctg aaaaaatcat tgatgcttat ggtattccgc acatctcaac
    41821 aggagatatg ttccgcgcag cgatgcaaaa cgaaacagct cttggcctgg aagcaaagtc
    41881 ttatatggat aaaggtgcat tagttcctga tgaagtaaca aatggaatcg tgaaagagcg
    41941 actagctgaa ccagatacag agaaaggctt cttattagat ggctttccac gtacgctaga
    42001 tcaagcaaaa gctctggacg caatgctaaa agatttgaat aaaaaaattg atgctgtcat
    42061 cgatatccat gttggtgaag aaatcttggt cgaacgtcta gccggccgat ttatctgcag
    42121 caattgtgga gcaacttacc ataaaatttt caaccctaca aaagttgaag acacatgtga
    42181 tcgttgcggt gggcatgaat tctatcaaag agaagatgat aaacctgaga cggttaaaaa
    42241 tcgtctggct atcaacatca aaaatagtga accaatcttg gcttattata aagaacaagg
    42301 tttgctaaac actattgatg gtgctcgtga aatcgatgct gtatttgcag atgtacaaaa
    42361 aatcattgaa aaatagcacg attgcccgta gccgacttgc attatgcagt tactcttgtc
    42421 aagttcaata aattatgata gaatatgtca gtccgacttc tagagaatcc tctagatgat
    42481 ccattttgag aattttgagg tgggaactgc agtttccgcc attttatcgt gtgaaaatgc
    42541 gaaacaagta caaggaggta ctgtgcgtgg caaaagaaga tatgattgaa gtcgaaggta
    42601 cagtcgtcga aactttgccg aatgcaatgt ttaaagttga actagaaaac ggacaccaag
    42661 ttcttgctac tgtttcaggt aaaattcgta tgcattacat ccgtatctta cctggtgaca
    42721 aagtaacggt tgaattgtct ccatacgatc ttaaccgtgg ccgtattact tatcgcttta
    42781 aataattgta ctccgtaaaa ctacaggagg tattatcatg aaagtaagac catcagtaaa
    42841 accaatgtgc gaacattgta aagtaattcg tcgtaaagga cgcgttatgg tgatttgccc
    42901 agcaaatcca aaacataaac aacgtcaagg ataattggag gtgctataaa tatggctcgt
    42961 attgcaggag tagatattcc tcgtgataaa cgtgtagtag tttcacttac ttatatctat
    43021 ggtattggta acactactgc taaaaagatc ttagctgatg ctggtgtatc tgaagatgtt
    43081 cgcgttcgtg aattaacgaa tgaacaaaca gatgctatcc gtgcagaagt agataaatta
    43141 aaagttgaag gggatcttcg tcgtgaagtg aacttgaaca tcaaacgttt gatggaaatc
    43201 ggttcttacc gtggaatccg tcatcgccgt ggattgcctg ttcgtggaca aaacacgaaa
    43261 aacaatgcac gtactcgtaa aggcccagct cgcgctatcg ctggcaagaa aaaataattc
    43321 ttaagtgaag gaggttaaaa cttcatggca gcaaaaaaag tgaatcgtaa acgccgtgtg
    43381 aagaagaata tagaagcagg tattgcacat atccattcta cattcaataa cactatcatc
    43441 atgatcactg atactcacgg aaatgcatta gcgtggtcat cagctggttc attaggtttc
    43501 aaaggaagca aaaaatcaac tccttttgcc gctcaaatgg cagcagaaac tgctacaaaa
    43561 gcagcaatgg aacacggatt aaagactgtt gaagtaacag ttaaaggacc tggttctgga
    43621 cgtgaagcag caattcgttc attacaagct acaggtttag aagtgactgc aattcgtgac
    43681 gtgactccag ttcctcataa tggtgcccgc cctccaaaac gccgtcgtgt ttaatgagtg
    43741 ccgctcattt tgagttttat cagtaacgtc attgaacaag acacttttcg ttttgaaagg
    43801 ggtaaagata agaatgattg agttcgaaaa accaagaatc gcaaaaattg atgaagaaaa
    43861 agattatggc aagttcatcg ttgaacctct tgaaagaggg tatgggacta ctttagggaa
    43921 ttccctacgt cgtattttat tatcttcttt gccaggtgct gccatcacta atattcaaat
    43981 cgatggtgta ctgcatgaat tctctaccat caaaggtgtt agagaagacg tcactcaaat
    44041 catcttgaac attaaaggct tagcgctgaa aatgtatggc caagaagaaa aaacccttga
    44101 aatcgatatt accggacctg ctacagtaac tgctggcgat attatcgttg atagtgatgt
    44161 agaaattctt aacaaagata tgtacatctg tagtgtctct gaaggagcta cgttccatgc
    44221 tcgcttaaca gtgaaacctg gacgtggtta tgttcaagca gatgaaaata aaaaagaaga
    44281 tatgccaatt ggcgttcttc cagtcgattc aatctacaca cctgttcgtc gtgtgaacta
    44341 tcaagttgag aacacacgtg ttggacaccg tgaagatttt gataaattga cgatggagat
    44401 ttggacagat ggttcaattg aaccgttgga agcaatgagt ttgtctgcga aaatcatgac
    44461 tgaacatttg gatatttttg tgaacttaac tgatgaagca aaaaatgctg aaatcatggt
    44521 cgaaaaagaa gaaacacaaa aagaaaaaat gttggaaatg acgatcgaag agttagactt
    44581 gtctgttcgc tcttacaatt gtttgaaacg tgcaggaatc aatactgtac aagaacttac
    44641 aaataaatct gagcctgaaa tgatcaaagt ccgtaactta ggacgtaaat cattagaaga
    44701 agttaaagca aaattgcacg acttaggttt aggtttacgt aaagaagatt aaaacctttt
    44761 acgaaggagg gaaaccaacg tgagttatcg taaactagga cgcacatcta gccaacgtaa
    44821 agcgatgttg cgtgatttaa caactgattt attaatcaac gaacgtattg tgactactga
    44881 agctcgtgcg aaagaagttc gctcaactgc agaaaaaatg atcactttag gtaaacgcgg
    44941 agatttgcat gcacgtcgtc aagctgcggc tttcgtacgt aatgaagttg ccagcgtgcg
    45001 tgaagaaaat gaggacatcg tcattgaatc agctttacaa aaactgttca acgatattgc
    45061 acctcgttac gcagaacgtc aaggcggata cacacgcatt ttgaagacag aaccaagacg
    45121 cggtgatggt gcaccaatgg ttgtcttaga acttgtctaa aatcacacta ataacatcac
    45181 tttatagatg attcttttag agtgttatga tgttggaaga tatttttccg agtctagctc
    45241 agcgctgctc ctcaagcgaa tatcgcttgg ggagtaagca ctcatcataa agtgtttttt
    45301 tataccttca attacctgtc atgacagata tgtttgaagg cataaaaaaa cattttaagc
    45361 gagaaataat tgaaaatttt gtcttttttt gatagtgtta gtaggaactt taggatgaag
    45421 aaggagtaat catggagcca attattgaat tagaaaagat aaattataaa taccagccag
    45481 atgatcttcg ccccgcattg aaagatgtct cctttacaat tgataaaggt gagtggatcg
    45541 ctattattgg acacaatggt tcaggaaaat cgacacttgc caaaacgatc aatggattgc
    45601 ttctgccaga atcagggatc gtgaaagtgg gaaatcaaat attggatgaa gaaaatattt
    45661 ggacgatccg acagatggtt ggaatggttt ttcaaaatcc agacaatcag tttgttggtt
    45721 cgacagtaga agatgacgtt gcttttggtc ttgaaaatca agggattccc agagaagaaa
    45781 tgctagtgcg tgtgaaagat gcacttgaga aagttagaat ggctgagttt gcttcacgcg
    45841 agcctgctcg tttatcagga gggcaaaaac aacgtgttgc cattgccggc gtggttgcct
    45901 tacgacctga tatcattatt ttagatgaag caaccagtat gctagatcca gaaggtcgtg
    45961 aagaagtgat ctctacgatc aagaagatca aagaagaaag tcaattgacc gttatatcta
    46021 tcactcatga tattgatgaa gcagctaatg ccaatcggat actagtgatg agacaaggcg
    46081 aacttgtccg tgaaggtaca ccaaaagaaa ttttttctgc tggtcctgaa ctgatcgact
    46141 taggtctaga tctaccattt cctgaaaaac tgaagagcgc actgaaagaa cgtggggttg
    46201 atgtgcccag tgaatatatg acagaagaaa ggatggtgga ttggttatgg acatccgttt
    46261 tgaacaagta gacttcactt atcagccgaa tacaccattt gaacaaagag ccttatttga
    46321 catcaatatg acgatcaaag aaaacagtta tactgcatta gtagggcata ccggaagcgg
    46381 gaaatcaacc ttacttcagc atttgaatgc gcttgttaag ccaacaagcg gtacagtcca
    46441 tattggagaa cgagatattc agccggatac ggataataag aatttaaagc ccatccgaaa
    46501 aaaagttggc atcgtctttc agtttccaga agcacagcta ttcgaagaaa cggtagcaaa
    46561 agacattgct ttcggtccta aaaactttgg tgtcagcgaa gaagaagcac tagtcctagc
    46621 aaaagaaaca ttagaacaag ttgggctgga tgaaagctat ttggaacgtt cgccatttga
    46681 actttcagga gggcaaatgc gtagagtagc aatcgctggt gtgcttgcca tgaggccgga
    46741 agtacttgta ttggatgaac caacggcagg acttgatccc cagggcagaa aagaaatgat
    46801 ggagatgttt tggcgtcttc ataaggaaca gcagatcact attgtattag ttacgcattt
    46861 aatggatgat gtagccaatt ttgcagatta tgtctatgtg ttggaaaagg gacgaatcgt
    46921 taatagcggg gagccgcaag aggtctttca aaacattgaa tggctaaaag aaaaacaact
    46981 aggcgtacct actgccaccg agtttgcaga agaattaatg gctaaaggga tgaactttgc
    47041 taccttgcct ttgacagctg aagaattagc agacgctatc gtccattatg ctggaggaaa
    47101 tgcccatgat gaataaattg atatttggtc gttatatgcc tggagattcc ttgatccatc
    47161 gattggatcc ccgagctaaa ttattagcca gtttttattt tatcggaatc atttttttag
    47221 caaataattg ggaaacgtac cttttgttgg catttttcac attactttct gtcttcttgt
    47281 caaaagtaga tttgaaattt tttatccgag gcattcgccc attgatctgg ctaatcctct
    47341 tcactgtagc tttgcaggtc ttgtttacaa gtggcggaga aacttattgg agttggggtg
    47401 ttttccatat cactgaattt ggtgtgatga acggcctctt tattttctgt cgattcgtgt
    47461 tgatcatttt tatgtcgact ctactcacgc ttactacacc gcctttggaa ttatcggatg
    47521 cgattgagta cttgcttcgt ccattgaaag ccgtacggtt cccagtccac gaaatttcat
    47581 tgatgctgtc gattgctctt cgttttgtgc ctacattgat ggacgaaaca gaaaagatca
    47641 tgaacgctca gcgcgctcgc ggtgttgatt ttggtgaagg aaacctagtt cagaaaatga
    47701 aagcagtcat tcctttgctg attcctttat ttgtcagcag cttcaaccgt gcggaagatc
    47761 ttgcgactgc tatggaagca agaggctaca aaggtgggga aggaagaacg aaatatcgtg
    47821 tattgcattg gcataataga gacacgatgg ttatcattgc ttttgtatta ttaactgtgc
    47881 tactgatctt tttaagagga taaacgttca gtgggccgct atctattctt ttcttgctga
    47941 tctgtttgca agaaaggatg caagcagtgt atgacaagaa atattagggt ctgtggcaga
    48001 ctaaggtcac aggcctcttt cttttatctg aaaagaaaga acgaaaaa
//
LOCUS       DAZKOW010000008        46206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000008 DAZKOW010000000
VERSION     DAZKOW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.008"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACG912_000484"
     CDS             complement(<1..132)
                     /locus_tag="ACG912_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0511831.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
     gene            542..781
                     /locus_tag="ACG912_000485"
     CDS             542..781
                     /locus_tag="ACG912_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511832.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            848..1057
                     /locus_tag="ACG912_000486"
     CDS             848..1057
                     /locus_tag="ACG912_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511833.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            1117..1788
                     /locus_tag="ACG912_000487"
     CDS             1117..1788
                     /locus_tag="ACG912_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0511834.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1841..3817
                     /locus_tag="ACG912_000488"
     CDS             1841..3817
                     /locus_tag="ACG912_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0511835.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            3830..4585
                     /locus_tag="ACG912_000489"
     CDS             3830..4585
                     /locus_tag="ACG912_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0511836.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            4582..5406
                     /locus_tag="ACG912_000490"
     CDS             4582..5406
                     /locus_tag="ACG912_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0511837.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            5416..5664
                     /locus_tag="ACG912_000491"
     CDS             5416..5664
                     /locus_tag="ACG912_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511838.1"
                     /translation="MKIIRILVFNLSFLAATILCCGLIFSQLSIYPRYQRLFIHFLNI
                     QCASMPLEVIKKLLINPIFMVSLVLLTGLVTWVLGKKE"
     gene            5707..7806
                     /locus_tag="ACG912_000492"
     CDS             5707..7806
                     /locus_tag="ACG912_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HFQ0511839.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(7844..9901)
                     /locus_tag="ACG912_000493"
     CDS             complement(7844..9901)
                     /locus_tag="ACG912_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFQ0511840.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(10015..10227)
                     /locus_tag="ACG912_000494"
     CDS             complement(10015..10227)
                     /locus_tag="ACG912_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511841.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            10414..10881
                     /locus_tag="ACG912_000495"
     CDS             10414..10881
                     /locus_tag="ACG912_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511842.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            10878..11120
                     /locus_tag="ACG912_000496"
     CDS             10878..11120
                     /locus_tag="ACG912_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HFQ0511843.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            11138..11440
                     /locus_tag="ACG912_000497"
     CDS             11138..11440
                     /locus_tag="ACG912_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511844.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            11512..13566
                     /locus_tag="ACG912_000498"
     CDS             11512..13566
                     /locus_tag="ACG912_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511845.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNE
                     VK"
     gene            13566..16178
                     /locus_tag="ACG912_000499"
     CDS             13566..16178
                     /locus_tag="ACG912_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFQ0511846.1"
                     /translation="MRIRETTRRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     QKIFYSGLAYLPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWN
                     PLTPTTSLGTRLVVSTICVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            16175..18706
                     /locus_tag="ACG912_000500"
     CDS             16175..18706
                     /locus_tag="ACG912_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFQ0511847.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            18758..19090
                     /locus_tag="ACG912_000501"
     CDS             18758..19090
                     /locus_tag="ACG912_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511848.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            19091..19684
                     /locus_tag="ACG912_000502"
     CDS             19091..19684
                     /locus_tag="ACG912_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511849.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            19847..21541
                     /locus_tag="ACG912_000503"
     CDS             19847..21541
                     /locus_tag="ACG912_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFQ0511850.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            22229..24106
                     /gene="ltrA"
                     /locus_tag="ACG912_000504"
     CDS             22229..24106
                     /gene="ltrA"
                     /locus_tag="ACG912_000504"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0511851.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            24237..24614
                     /locus_tag="ACG912_000505"
                     /pseudo
     CDS             24237..24614
                     /locus_tag="ACG912_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            24614..25819
                     /locus_tag="ACG912_000506"
     CDS             24614..25819
                     /locus_tag="ACG912_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0511852.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            25835..26479
                     /locus_tag="ACG912_000507"
     CDS             25835..26479
                     /locus_tag="ACG912_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511853.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            26490..27296
                     /locus_tag="ACG912_000508"
     CDS             26490..27296
                     /locus_tag="ACG912_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511854.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIFYIH
                     EDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            27318..27548
                     /locus_tag="ACG912_000509"
     CDS             27318..27548
                     /locus_tag="ACG912_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511855.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            28094..28369
                     /locus_tag="ACG912_000510"
     CDS             28094..28369
                     /locus_tag="ACG912_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511856.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            28411..28833
                     /locus_tag="ACG912_000511"
     CDS             28411..28833
                     /locus_tag="ACG912_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511857.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            28852..30444
                     /gene="mobP2"
                     /locus_tag="ACG912_000512"
     CDS             28852..30444
                     /gene="mobP2"
                     /locus_tag="ACG912_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobP2 family relaxase"
                     /protein_id="HFQ0511858.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            30456..30791
                     /locus_tag="ACG912_000513"
     CDS             30456..30791
                     /locus_tag="ACG912_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511859.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            30938..31132
                     /locus_tag="ACG912_000514"
     CDS             30938..31132
                     /locus_tag="ACG912_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511860.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            31164..33536
                     /locus_tag="ACG912_000515"
     CDS             31164..33536
                     /locus_tag="ACG912_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFQ0511861.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            33597..35057
                     /locus_tag="ACG912_000516"
     CDS             33597..35057
                     /locus_tag="ACG912_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFQ0511862.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            35068..37272
                     /gene="topB"
                     /locus_tag="ACG912_000517"
     CDS             35068..37272
                     /gene="topB"
                     /locus_tag="ACG912_000517"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0511863.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            37288..37437
                     /locus_tag="ACG912_000518"
     CDS             37288..37437
                     /locus_tag="ACG912_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511864.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            37511..38104
                     /locus_tag="ACG912_000519"
     CDS             37511..38104
                     /locus_tag="ACG912_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFQ0511865.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            38120..39019
                     /locus_tag="ACG912_000520"
     CDS             38120..39019
                     /locus_tag="ACG912_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFQ0511866.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            39022..39507
                     /gene="ssb"
                     /locus_tag="ACG912_000521"
     CDS             39022..39507
                     /gene="ssb"
                     /locus_tag="ACG912_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0511867.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            39873..40133
                     /locus_tag="ACG912_000522"
     CDS             39873..40133
                     /locus_tag="ACG912_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511868.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            40574..40780
                     /locus_tag="ACG912_000523"
     CDS             40574..40780
                     /locus_tag="ACG912_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFQ0511869.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            40780..41031
                     /locus_tag="ACG912_000524"
     CDS             40780..41031
                     /locus_tag="ACG912_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511870.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            41046..41483
                     /locus_tag="ACG912_000525"
     CDS             41046..41483
                     /locus_tag="ACG912_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511871.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            41476..42183
                     /locus_tag="ACG912_000526"
     CDS             41476..42183
                     /locus_tag="ACG912_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511872.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            42348..42548
                     /locus_tag="ACG912_000527"
     CDS             42348..42548
                     /locus_tag="ACG912_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511873.1"
                     /translation="MNKIIPLLFVLFVGEILMRHAAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            42564..42743
                     /locus_tag="ACG912_000528"
     CDS             42564..42743
                     /locus_tag="ACG912_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511874.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            43084..43347
                     /locus_tag="ACG912_000529"
     CDS             43084..43347
                     /locus_tag="ACG912_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFQ0511875.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            43341..43691
                     /locus_tag="ACG912_000530"
     CDS             43341..43691
                     /locus_tag="ACG912_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0511876.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(43715..44329)
                     /locus_tag="ACG912_000531"
     CDS             complement(43715..44329)
                     /locus_tag="ACG912_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0511877.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            44643..45065
                     /locus_tag="ACG912_000532"
     CDS             44643..45065
                     /locus_tag="ACG912_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511878.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            45075..45278
                     /locus_tag="ACG912_000533"
     CDS             45075..45278
                     /locus_tag="ACG912_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511879.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            45489..46073
                     /locus_tag="ACG912_000534"
     CDS             45489..46073
                     /locus_tag="ACG912_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0511880.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT        16937 a         7300 c         9359 g        12610 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaaatgata ttttacgagg aggaatagta
      541 gatggcgcga ccagttttag aggcgatgga tatggatact ttgacaaaag gaaggtatgt
      601 acagctgtta gctcgtggtt attcacctaa agaagtattc aaagccttat ctaaaggaac
      661 agatatggaa aaagaaagat tgcgaaaaga attcgattat tggagaaccc ataatggaat
      721 caaggatttg aaaccagcac ggccagtaat taaacgaaag aaaaagataa cccaagactg
      781 aagtgtagag cgtatgtgat cataggcttt tttatttttg caaaaaacaa ggaaggtagg
      841 aaaactcatg aattatgaag aaatcgaaaa cagaaaaaaa gtgtcgaaag aaatggaaga
      901 aaagttacta aaaacgatga agcaaaaaca ccttaaaaga ttatctgtcg cgcaatatat
      961 caatgatatg cagttaacag gaaaagagaa agcttgtttg ttaggttcaa tgaaaaactt
     1021 tgaacagttg agacgaactt atagaattca tttttaaaac tttttctatg gaaatgagaa
     1081 aaagtttttt tgttttgtct gaaaggaggc agatggatga aaaaatggtt atttggtttt
     1141 ttaggagtca ctttaatcgt cttttgcagt gtgtttggct atgttagcta ccaaaaacat
     1201 gaaggagagg tgtttaagca aaatatagaa aaaaagatgc ccgtagatca aatcaatgca
     1261 catgcaaaga gttataagga ggatgcaacg aacgtcaata atgatatgtc tttggggcaa
     1321 atgttgtcca tacaaaaaga agcaatcgaa atgggcgtca ataaacaagt atttgcacaa
     1381 attcagattc ctgcacttgg tttagcattg ccaattttta aaggggcaaa tcaatacact
     1441 ttgagtcttg gagcggctac ttatttttat gaagatgccg aaatgggcaa aggaaactat
     1501 gtcctagcag gtcataacat ggaaatgcca ggtgttttat tttctgatat tcaaaagttg
     1561 tcattagggg aggtgatgga cttagttagt aacgatggcg tctatcgtta caaggtaacg
     1621 cgtaaattta tcgtacctgg gtactttaaa ttaatcgatg gcgttcctga agagaactct
     1681 ttcttgtctt tgccaaagaa aggagagaaa cccttattaa cgttgtttac ttgtgtttat
     1741 acgtcacaag gaaaagaaag atacgtggta cagggagagc tacaataaca aggttttaat
     1801 caacgcgagt cgttgaaata tgaaatgagg agagtaaaaa atggcaataa atcggtatgt
     1861 aaaaaaagct ggaatgcttg ttggaatgtt aggaatcatt gcaggctgct ttcaggggta
     1921 tcaagtacat gcagaagatg tcacgcaaaa aacaccacca gagaaggtca atatcacggt
     1981 tcataaatta atgtacgatc aaggaacgca attgaatgtt gatattgacg ggatcaaaaa
     2041 cgatggttat acgcacgatc aatatcctac aggtgtaacg aaatacaaca aggcagatta
     2101 tggtgatgtt gagtttacgt taggaaatat tacggatcaa gtattaccga gagaagattc
     2161 tgatctaacc aacgcgaaag tggacgagat tgtacaagat gtagaagaca agggatctaa
     2221 cagtgagtac gtcaagaatg caaccaacaa aattacttca gctgtagatg aaaatggtga
     2281 gatcactttt actgatcaac cagcctatgt caattccaaa gggaatgtat atgtggttta
     2341 tgaaagtaaa tccgctgctg gattagtcac acaaaaagca aaaccaatgg ttgtgattgc
     2401 gccaatgaca gataaaacag gatcaggatt tttaaaaaat attcatttgt atcctaagaa
     2461 cattgtttct aagttgagtt ttgaattaac gaaatttggt gacgatggcg cagaccaaag
     2521 caaacaaacc cttttaaaag gggcaaaatt tgagttatac aagggcgaac ctggaaaagg
     2581 aacaaaattg ggtgatcttg tatcggatga tcaaggaaag ctgactgcga ctgatttgac
     2641 gttaggaaaa tactattttg tggaagttcc ttcggaagtt gtagtgggat cagatcaaga
     2701 accaacagca gatcaatatt tattaggcgc agacgctcgc aacgatgcgc acaataaatt
     2761 aacctttgaa attacgaatg atggcgtgac aagtgactta aaagcttcat acgtaaacta
     2821 taaagcacca gtgatcgata aaacagtaac aaatggaaca ggacaagaac attctttcca
     2881 aattggtgac gcagtaaatt atcaaggaac gattcatatt ccaacagaca ttgcaggtgg
     2941 cgcagacggt atcacagtaa acggtgtgaa aagcgaaact tctccttaca gtgtatttaa
     3001 atggggagat acagcaggta aaggactttc ttatgttgct gaaaaagcag atattaaagt
     3061 aacaaataat gatggttcag tcgtattaaa agaaaatact gactataaaa tcgaaaattc
     3121 tgatagtggt tttgtgatcg attttatcgt aaataatgga caagtaagtg atacagttgc
     3181 gaatttacat ggtcaagatc taaaaatgac ttataacatg tttgtgaatg atagtgctga
     3241 aatcgcaaat ccattaacaa atagcgtgga tttcgtttat aacaataatc catttaatca
     3301 ggaagaacac cacgaaaaaa cgaaagctga cgttgtgact tatggcgcaa aattcttaaa
     3361 agtcgatagc ggcctatttg gaacaggtat caaagcaaca ccgcttgaag gcgcagaatt
     3421 tgcagctaag aatgcagaag gtaagtacta cggtggactt actgatacag ataaagatgg
     3481 tgtcaaagaa gcagtttggg tagacgatgt agcaaatgca gcaatcctaa aatcagataa
     3541 agagggtcat tttgaaatca ctggtttaac tgaaggagat tactcactgg aagaaacaaa
     3601 agctcctgaa aactatcaaa aattaacaaa agagatctct ttcaaagtag acaaagattc
     3661 atacaaagaa gaaaaccgaa tcacgatcaa aaataatcaa aaagcttccg taccaatgac
     3721 tggtagcaac ggtttccaat cttacgtatt gatctcttgc ttgttgctag gagcaggggg
     3781 actttctgca gttgtttact ttaagaagaa agcctagaag gatcagctaa tgattattcg
     3841 taagccccca aaaagaagag tcatgcacaa actgatgata tttttttggt tgctgtcggc
     3901 agtagcagtc tatttaattc cagttgtgga aacaaatgta gcgaaatttc aacaacagca
     3961 aagaatttta gccattgaaa atcttgaaaa aacagattct tctttggatg ttgcgaaaat
     4021 agagttaggc gatcctgttg gtgttcttac aattccaagt atttctttaa aactaccaat
     4081 ttatgacgga acttccgaca aaattttaga gaatggtgta gggattacgg aaggaactgg
     4141 cgatattaca ggaggaaacg gtaaaaatcc tttaatcgcg gggcacagtg gactttataa
     4201 agataatctt ttcgatgatt tgccttcagt aaaaaaaggg caaaaattct ttattaaggt
     4261 aaatgaagaa caacatgcct atcaaattta tcgaattgaa gaagttaaag cagaagaatt
     4321 acaaaaaaat tatttagatt atcttatgcc tgaaaaagac caagatagga tcacgttaat
     4381 gacatgcaca ccaaaaggtg tcaactcaca ccgcttttta gtgtatggtg aacgagtttc
     4441 cttcagtaaa gaagatttaa aactacagaa aaaagcaaaa aataggaaat caatttcatt
     4501 ggattggcga ttagcactgc tattcgcttt tttttgcgtt cctattattt ttatttataa
     4561 aaaaataagg aggagatcgg aatgaataag agaaaatggc tgtttgtttg tgtagcagct
     4621 ctcttttcta ctgtagctta ttcgcaactg tctttcgctg acagcagtga tagtagtacc
     4681 actcaaacaa tagataattc aattactgca ccaagcgaaa cggacagttc ggagaatact
     4741 acagaagaaa cggcagattc aaatataagc tttactgaat caacgaatcc aacgtcagaa
     4801 agttcttcag agacttcaga aaagccgcaa gacgtagaag atacgcaagt gatcaagcaa
     4861 gccattcata ttcaaaaaat tatttcttcg aatcaaatca acaatcaagg cagtcaagta
     4921 gcccagcaag aaggtatcaa tggcgcgaag tttattgtct acgatatcac gaatatctta
     4981 gaagaaatgt cacaagatga taatgagaaa aatcaatctg tagaaataat tgagtcaaaa
     5041 ttgaaagatc gagcaaaaaa actttcatac gaccagttga aaaaagtggc tgaaggagaa
     5101 accaaaacga ttgatgatca ggaaggcgtg attgaatttt ccattgaagt acctgcaaac
     5161 aaaaaacaag catactatat cgttaatgaa tcaagccctg aaaacatttc aaacagtgaa
     5221 gatattgttt tattgacacc tgtttcaaat gaaaaaggtg aatttttgac ggatgtttgg
     5281 ttatacccta aatcagagaa atcagagccg aaagaagaaa ttaaaaaagt tgtgtcaact
     5341 ggtgtaaaga agaacttttt tgaaaattgt tgggaatttt ttgctcaatt attttttgat
     5401 aattaggatg aaatgatgaa aataataaga atattagttt ttaatctatc ctttttagcc
     5461 gcaacaatat tgtgttgcgg tttaattttt tctcaattat caatttatcc aagatatcaa
     5521 cgattgttca ttcattttct aaacattcaa tgtgcttcca tgccattaga ggtgattaaa
     5581 aagctcttga tcaatccaat ttttatggtt agtttggttc ttcttacagg attggtaacg
     5641 tgggtattag gaaaaaaaga atgaaaagct tacgatcgtt taaaaaaata ttagatggag
     5701 ggcattatgg aaaataaaat tcattgcact acgggaagag taattacttt agtgttgttt
     5761 ttgttccttc ttctttttgc gaggggaact gcagtccagg ccttagaaaa agttgatccg
     5821 agtcaaatca aagaaaataa taatctggtg ctttatcatg atagtgattc attgagaaat
     5881 atcgaagtaa ctgggatcaa tggatttaag atactagatg agtattcaac taaaaaacaa
     5941 aaagtacttg cttggccaaa agtcaaagat ttgaaaagta cgtcaaaaat cgttgcccat
     6001 tatcaaaaag taggtactta tctaggaaaa gaaattgatt gtgacgtgac tttttctaat
     6061 tttgtagcgc acgctggggt taaccaagac ggattagcta aaacaattaa tggcaaaaag
     6121 tattataatt atttgctctt tgataaaaat atttatcgag gatttttcat gcagtcaatc
     6181 aatgtcatga atgcaaaact agaatttaag tatgcaggaa caaaaacaaa agtagctttg
     6241 agtgaaccac agccagaaat tcaatcaaaa atgagatcga tgagagcagc tggtattgat
     6301 ttaaacgata catttctatc catttacagt ttaaatggac agtatcagca acgtgtgtct
     6361 tggtataatg acaataatga atctgtcggt gaacgtaata ttgatatgag agaatttgtt
     6421 gggtatgaaa aaatgggtag cttaccttat tttgtcacaa cagatacagc atgtgcagaa
     6481 tacaaagctc ctgcgttatc aacaaacaat ttaacttcaa aagtagtggg aggacgtgca
     6541 gaaaaggctt atagctcgaa tgatcatttc accgatgttg taggagctga tacttatcac
     6601 agaagtggtg taacgtatac gcttcaaggc gcttccccaa cattcatgat tggcgcaaat
     6661 acgaatagta tgatgtttag ctttgatact gcattgctat ggacaccaca accatcgaag
     6721 cctacaaaag aagtgtttaa caaagctaat actgaagagg cagcacacaa tattgacaaa
     6781 aaagtgattc cacaaggatc agatgtttac tatcatattc accaaaagtt tgatgcatta
     6841 acagtcaaca caatgaacaa atacaaatca tttaaaatca ctgatacctt tgacagcaaa
     6901 aattttgata tggtatcgga tgggaaaaac tatgatggcg cattgtggta tgttgaagcg
     6961 aatggtactt ggaaaaaagc tgatacagga ggaaagttaa ctgtatcagg taatacagtc
     7021 acttatactg cttctgacag ctttttagct aacgctgcaa attacggtag ggattatatt
     7081 ttgcagctcc atatgagagc aaaaaatgat gcaaaaggaa ttgcaccaaa tgtggctact
     7141 gtaacgttta acaatgatcc gaacaaagga acacaaaaaa caaatacagt agaaaactat
     7201 ttctctctta aaccaaataa aaaagtggaa caagatggga aagatgttga tggtcgaaat
     7261 gatggtaaaa aaggaacgcc tacagctcct ttaaatgcag gatctgaagt acaatatcta
     7321 gtcacacaaa aatggcatac aaaaggtgta gacattgcct cagatcatta caagcaattt
     7381 agtattcaag atccagtgga agatcgtttg acgtataaag aaggttctgc gcgagtgata
     7441 gatatgagta caggaaaaga tattacttca gaaggtacac taacctatga tagcaattca
     7501 agaacgctga agtgggaagc ttccgctgat ttcttaagta aaaatctttt agatggacga
     7561 gaaattcagc tgatatttac agctaaaact cctttgaatg aagaattgga tatcgacaac
     7621 caagcaaaag tttcagtaga aagtattgag aaagcaacta atgtagtaac gattggggta
     7681 aaacctaact tacctcagat cattgtgcca aaaactggat cgaatcattt agtgctaact
     7741 gttattggaa gtatattatt tcttacttta gcatcattgg gctatgtagt tttaaaactc
     7801 aaataaatat aaaaacaagg ctgagaatca gccttgtttt cttttatttg aagattggat
     7861 tcgcactaat aaatacatta tctacgccaa ttctaccagc agagtcaact acaaaattat
     7921 gacctaatcc gagaatactt tggttattat aaagtaaatt tgcattatgg gcagggctat
     7981 ttttccatcc taccatggca tcgatagcac catcgtctga taaaaggtca tcaatataca
     8041 gcccagtttg acttatgttt tctcccttag caccgtagca gcctaagctt tccaataaat
     8101 cttggaaagt accaccacca gggcgatagt gactgaagct gacttgtaat tctttagctc
     8161 gaatatctgc agcctgttga acaatatcat tgaattgcat tggggcaaca ccattttgtt
     8221 ggcgataatc atttaaccaa gcaagttctt tttgacgcat aaagttacgt aattcgtcta
     8281 atgaaatctt tttatatttg aatgtgattg ttttattttg atcgacgtta ttaatggatc
     8341 gttggccgtt tttatccacg gtaacggtag gatcgtcatt tctgcacaag aaataatttt
     8401 gatcagtcat gtttttccaa ggagtagcaa agctatcccc ttcgtatact gtttttgatt
     8461 cactagagag aacagcgttg gtatcagcat ttacatgatt aacagtcaca ttaaatttac
     8521 gagtgtagtt gaaaatataa ttttgacttt cagtcacatt gtttaaggtt actgaatttg
     8581 ttggttctac ccgatcgtct aaaacatatc cttgaataga aggagcagtt aaagtgacac
     8641 tgtcaccgtg agtgacattg actatctgtt ctttcaaaat attaactcgt ccttcgtttt
     8701 tgtagttgtt attgcacaac aaattgattt gatgcacttg attataattg aatgtgattg
     8761 ttgtattacc tgtgattgtt ttgctttgag tatcattacc aatcactttg tagctaaaat
     8821 tatctttagc gccttcgcta tatttaaatg cttttgcctt agcagtgtaa gtagaaccag
     8881 aagttacttt tgcttgatct gtagcaatca cttgacctgt ttgattgtca atatggttaa
     8941 tagtaaccgt atattctttt ggtgtttcag gtttgtgttg caacccttta aatgcagcat
     9001 caagactatt tttagcattg tttacttgtg tttgagaaac cttagaatcg tctagtactt
     9061 ttttagcatt ggataaagca gtctgaaaat tattccaaga ttcttctgta aaatctcctt
     9121 tagcagttga ttttactcgg ttgtataaag cttgtaattc agctttattc acagtaggaa
     9181 ctggtttttt ctttaatcct ttgtaagcag aatctaattt atttttggca ctatccactt
     9241 gcttttgagt cgcttttgag tcatttaaca ctttcttagc attgtttaaa gcggtttgga
     9301 atgcgttcca ggtattgtcg gtatagtcac cttttttcgt tcccttaacg ctcgtataaa
     9361 gagcttctag agccgattta ttcactttgg gtttagaggt gtcagcgcca taccaaccga
     9421 taccttcgta tttccagcca tagtctttgg ttaaccattg tacttctttc caacttgcag
     9481 tgtagtgatg tgagccagat tttacaccag gaaggaaaag gcggtagatc ggtttattat
     9541 ttttttcggc agaacgccag gcaggccctt cataagtcca gccgtaattt ttagtcaacc
     9601 atttcacttc atcccaacta gctgtgtaat ggtgatcacg aagaccttta ttgtacaaac
     9661 gatagacaag atggccaccg ctagtcggtg cttcccaggc ggggccttca taatttcccc
     9721 attttatctt tactagatgg tcacgttctt ttttgctaga ggtgtaaaaa tgttcattat
     9781 tatttggatt gtaaaggcga tacatgacgt tagttgaagc agcgtcaaca gatccagcaa
     9841 gactaagtga ggcagcaaat gttccaatag ctaaaacagt ttttggatta aataatttca
     9901 ttttttcaac tcctaaattt aattaaaaat gtttacatga ttattatata tcggctaacc
     9961 gatatagtca ataaaaaata agaaaagcct tatataattt tggcttttct tattttattt
    10021 gttagttgaa gtattaatat caacgatttc ttcagccaat ttatttaatt tcatagcatt
    10081 tatgaatctc atgttgtcta tactagagcg tttgttggca aggtcactta ctgtagttaa
    10141 gggtacgcca gattctatgg atatagaacg aactgattta ccactatcaa acagcaacca
    10201 ttcgattttc tttttatcag cgtacatttc aatgctcctt cttgtttttt ctttattata
    10261 gctctttttt gtaatgaatc acaatatcaa gagaaaaatt ttctatagaa aggtggattt
    10321 ttatgagtat tcgttctccg tgtatccaaa tgaggtttgt tgctactttc gttatttgat
    10381 atactaaaaa taaacaacga aagaaggaat aaaatgaaga aaaagcaact atggtttgtc
    10441 tctatgtttg caatattttt tattatttta agtggatgtt caagtaataa gaaatcggaa
    10501 gctaaagaag ataatagttt attgcaacca ggaactgttt ggaaagaaga agtaggtggt
    10561 cttattcata atttaaaaat tatagatgat actacctggg aatattctga aagtgtatgg
    10621 catccagatc ctgtacaaat aacagttaag aggcaaaaag actatgaagg tttagaaaga
    10681 tacaaaatag tagattctgc aggcattagg gaatttataa acaaacctga ttcattattt
    10741 attgttgttc cctatgagaa ggatggcgta aaaaaaataa tttttcttgg atctagcaag
    10801 gatgagaagc gaacgaaaga gaaattaatt catgatggat cagagttaaa taaatacaaa
    10861 ttacaaaaga ctagtgagtg attgctagtc tttttattta tcctgaagga ggaatcacag
    10921 tggcaaaagt taaaacacca ttcgaaaaag ccgcgtctaa aatcttagaa cgccgtggtg
    10981 tttctgaaat acagtggaaa aaagaagttc ttgaaaaagc acaatttgac ctattacgag
    11041 gaaaagataa ggacttagaa gaatatatct atcaacgtga acgtgaaaaa cttgtattcg
    11101 atgaaattat taaacattaa aaggagcgaa taatcttatg tttgaatcaa ttacaatgaa
    11161 ttttttaact caaccgaaag tagtagctgc agttgatatt tttagagcat tacaaactgc
    11221 cgcaactagt ttgaaaaata atctgatgat ttttgcggct gtagctgccg ttgcctccat
    11281 tgctcttgga ggtattttct tcttctttgg gcgaagagga gctgaaaacg ggaagacaca
    11341 cgtaggttat gtattgttgg gggtaggagt tattttagct gccgcttcta ttgtagtttc
    11401 aatgttctca atgtttggac aaaccccaca aggactataa aaaaaacaag taggaggagt
    11461 aaaaagtacg tggctacttt ttactccttt ttttatgaaa ggagaatatt catgtttttt
    11521 acgatttcag gaaatgcaaa attggataaa gagttaataa gaaaagcaac gaagaaagtg
    11581 ggaaagcgta cttccttttt accgaaaaca attaaatatt ctgtcattga aacggaagaa
    11641 cagctacatg tgttaggaca agtgttagaa caagagaatc aatcttctgt taaattgact
    11701 ctcacgttac atgataaaga aggaaaaata gaaaaggctg gcacagaata cgtttttggc
    11761 ccactggatt atcaaatttc agaaggtttt tatcctacat tgatagattt aatccgcctt
    11821 gatgtgttta aagatacgga tgaaactatg agtgtggaag agtcttatcg aaacgaaaaa
    11881 ctgctacaag ttttagaaga agagttagag agcgaccaaa gaaaagtgaa tcaagtgagt
    11941 gaagtcgaag taaatgaaga agtaccatgg gaacgaccaa tctttccaaa gaaggagtca
    12001 aaagaaaaag agacagtcta tgttgacgaa caagctgata ttccgactca gaaatcgata
    12061 tttgaggaag agaacgatgt gatgggtcaa ttggaggtag atccctttga agaaacaatg
    12121 gaggatgaag agacggacga agtagaagtt gatccagttg aagctaatga agagtcaaca
    12181 tggactgaag gggattttca ggaatatatt gaaccactta tcagtgaaga agtagaaaaa
    12241 aacgaaacag agatacaaaa ggcaactgta tttgattata ttttggatca atatccgcca
    12301 gagcatgaat ggatgaaaga aaaaaacaaa ttatttttaa atgagcttta ttcgaaatat
    12361 caattacccc aaacctatca agcattttta gagagtaaag aagaattaat ctcacagggt
    12421 aaaggcacac ttttagaaca gttagagtct gtccaatcaa caaattggca tgagcttgca
    12481 caagaagatt tattagccac ctttgaagaa cgtcaacaag aaacagatca agaaattaat
    12541 gagtatgcga cggaacagtc gtctaattgg gagaaagtaa aagaggaact aaataaagaa
    12601 gaacagcgag tgattgaaga agaagtacaa aaagtcaaag agcggtacga aaagaaacgc
    12661 gacaatgctt attccttgtg tgtagaaaag atcaacaatt ttactaagac gaatcaagaa
    12721 gcgttatcga aagaaaaaga aaacttgtta aaagaacgta ttaaagaacg aaaagaagag
    12781 ttctaccgcg gtttacgtac agataagcta aagattagca aagaattaaa tgaacaactg
    12841 aataagctat atcaaacaac gactgaagtt cttatggaaa aacacgaaca gatccagcaa
    12901 gaattacaag tacaaattcc aaaatggaaa aaagagtatg aaattcaaca acaagaattg
    12961 aaagaacaag aagaaaaaca acggcaaaaa gaaaaagagc gtgaacaatt agaaataaga
    13021 aaacgggaac aggatcttgc ggaacaaaaa atggcgatag aaaaacaacg gttagagcaa
    13081 gaaaaggaaa aagaagcagc tgctcgtaag gaacgtcaag aacaaatgga tacattgcgt
    13141 caagcacagt tgaattatct agcacaacct caaccagtag ctgctgttca aacgccagtg
    13201 aacaaccaag caaatttaac tccttcaccc cagcgttcct ttaacggatg gatggtggga
    13261 tgtattgcaa gtttaagctt gcttctaggt ggaggaacag tatttgcttt taatcatatc
    13321 aatcaatctt cacaggctga ttctgtagcc acaacggcgt caatcaagga agaagcccag
    13381 gaacaagcaa gaaaagagta tgaacaacaa ttagaagaat tattgaatcg taccaatcag
    13441 tcaacaacag agtcaagcat ggaaaatagc aaagagagtg atcctagtaa ggctaaagaa
    13501 ggatcaagcg attcaactgt acactcaaac acttccgaaa gttcagaatc aaatgaggtg
    13561 aaataatgcg tattagagaa acaacgagac gaaaagacaa acgattcaat ttagaaaaga
    13621 ataggtatct ttctcatttc tatcgctata ctccttcagg agaagagcta ccagaaaaac
    13681 atgacaaacg tatttctcaa aagatttttt atagtggctt agcttatctt cctttaaact
    13741 atgttgttac aagaatacca gaagcatttc atcgatttga gattattcca ggaagtttac
    13801 aaggagtgaa tatttttcag ttacaagaaa tgccgcagtt attccatttt gattcaactg
    13861 gtttttggaa tcctttgacc ccaacaacaa gtttaggcac acgtttagtt gtttctacga
    13921 tttgtgttgt tccagctgta cttatgggat tgaaaatgga catgtggttt cgtccgctag
    13981 cagatggaca aaaaggggat aatcgattaa tgaccttaaa agaaatccaa cagatttatc
    14041 cgcaaatacc tgaaacaaaa actacttttc caggttatgg aggtattcca atcacacatt
    14101 acaaaaaata ttggtacatt caaacagata ctgttaatac gtgtattgtt ggtacctctc
    14161 gatcagggaa aggtcagatt gaagtgttag ccaccattga caatttatct cgtgcagaaa
    14221 aacaaagttc aatggtcgtt aatgatccaa aaacagaatt atatgttggt agtaaagatg
    14281 aactagaagc tagagggtat gatgtatatg cctttaatat caccgatcca ctgcaatcaa
    14341 tgagtgataa tccattggca ctcatcgtga aatactggaa acgaggagac atagatactg
    14401 caacacagtt aaccaatact ttcacaaata cgatttatta tgatgccaac gcttcagata
    14461 acaaatactt taacgaaaac gcgcagaaag ctgtcaatgc gcttattttt gcgttgttag
    14521 aacaagctga tcaaacagga gattactcca aattgacacc gaataatctc gttgagcttc
    14581 tttctgagat tggcggtttt aattattatc cagatccaga caatgaattc aaacagttga
    14641 atgccttaga tgaatttatg aatcagttgc cacctggaaa cattgcaaaa aaacaatatg
    14701 gggcaacgaa aattggatct gataaagcga agggaaatat tttatcaacg gcaatcacgg
    14761 gattgaatcc atttactcta acgaaaattg caaagatgac ttctcaatca acgattgatt
    14821 ataaatcggt tgggtttcca aaatacttag atttgaaatt acatgaaagt cttttaaatc
    14881 aacgagtcgt agtagaattt tatcataagg gaaagaaatt tcatgaagaa cgtgtcaaag
    14941 taggctataa aggatttagt gaaatcaatt ttgattgtca tttagttgca ggtgatcaag
    15001 tttgtttaaa atacgatcat caaggaaaag aatatgtggc ttggtatgaa ttttctcaaa
    15061 gagtattgaa agacgaagag ggtaatatcg tttatcggaa aaaacgagga gaaacccatt
    15121 tgccagaatt agaaaaagaa gccgttctaa cattagatga ggaaaaaagt aacttattag
    15181 tagagtctat tcggatgcac tataccgata aaccaatggc tatttttatg cttacacctg
    15241 attatgatcc atctaaccat atcattgtta gtatttttct tagtcagtta tataaagaat
    15301 tatctacgca atgtgttaag actaaaggag ataaatgtca ccgcaggatt cattttattt
    15361 tagatgaatt tgggaatatg ccaccaatgg ataatatgga aggaatcatg acggtcacag
    15421 ctggccgaaa catgttgtgg gatctgttta ttcaatctta tcagcaactt tatgcgaagt
    15481 atggagaaca aaacgcagcg attatcaaag aaaattgtca gacacatatt tatattatgt
    15541 cgacagatga taataccatc gaagaatttt ctaaaaaagt ggggaataag acggttgaac
    15601 aagagaacag taccatcaat tcattaggta tgaatactca tatcaatcgc aatgttgatt
    15661 cagatcgtat cttaactcct gaaagaattt ctactttttt agaaggagaa acgattgtac
    15721 tcgctccttt aaaacgacaa gatttgaaag gtcataaaat acggccgtat cctattttta
    15781 atcatgggga aacgaatctt ccttatgctt atcaatatat tgaagatttc aatccatcgt
    15841 gggatatcaa tgatatagac atacattgta cacatgcgga cttggatctt aaggctttac
    15901 aaattgatta tgattatttc ctaccagatg aatatgctag aaatctctat catggactgc
    15961 atgatcaatt agaagaaaaa aacgaagaaa atatgtttta taaaatcgtt caatcttttg
    16021 agaatgagac ggctaaaaaa ttgttaattc aattttacga agaacaagca gcagatcgac
    16081 ttcaagaata tgttgaaaag ttgatcaaaa ataaagaaat ttctgaaatg attgggaaac
    16141 ggttgttatt ggaatctgaa aaggtggtga aagaatgatc attggaggaa ttttttctgg
    16201 catagccgac tggtggtcag gcttattcgg agaaggggat gcaaacccca aaaccgtaac
    16261 gaaagtctta caagattatg ggaattatct gcagtatcaa gatgtattta gttggatttg
    16321 gaacactttt ctgtggggat tgattaaagg attatataat ctcaatactt tactagaaaa
    16381 aaccatttat caaagctttg atttaaaaga cttattgaac gccgctgggg taaatgcact
    16441 atttcaaagt ttgattagta aggtattggc tcttttttgt gtcgtcacat taatttattt
    16501 aggcttgaaa ttttttatga gtaaacaccc accaaaagtc aaaaatatct tggttaattt
    16561 atttatggcc atgatcttga tttttggtgg tagttcttta atcgatgaag ggttaaatat
    16621 cagtaaaagc ttttacggag acgtcactgc agcaaataaa gcagataaaa cgggatcacc
    16681 tgcttttcaa attattcaaa ataatgtgat cgatgtcagt acgttactag caactgaccc
    16741 gaataaagta gaatccctac caacagataa aagaaatgct ttaacagcga aaaattttaa
    16801 aacagcgaat atcaatggga ttattactcc tgaacaagct aaaggtatgg caggtaatga
    16861 taaaagtggg ttatcagata tacaaaagga tcggttaaag gcattacaat accgattgga
    16921 actagatgat aatgggaaag aaattcctgt tcaaattagt gacgaaggct tagcaaaata
    16981 tgtctatact tcagggtatc gtcgatacag tagtaaacca ggtattattt tagctggtga
    17041 atcttctttg gcagtagcct atttatttat cctatttacg atcgttactt gcattattga
    17101 gctggtattt aaaaaattct atttagtgat tgccgcgtca acggactttg agacaggtca
    17161 acggatgaaa agtgcgatag aagatatttc tcaatccttc ttgttattgg cgtttacgat
    17221 tttagaatta cgaatctata ccttaatgct ttcaggtatc ggtgatttgc atgcggcagg
    17281 aaagatcaat ggattcttgt atgtcgttgc tttgattgta ctaaccattg cgctattcaa
    17341 aggctctcaa tcagttacga aaattttcgg cgttgataca agcctgaaaa acggcagtaa
    17401 ttctcttctt agcatgtttg ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa
    17461 aaaccttgca agtgctggta agagtggttt aagccattta aatgatattc gtaaaaatgg
    17521 atttggccat cgaaaagatg ttacccctga taacggggaa gaacaatcag acgataaagc
    17581 tacaactgta cctaatagta agaaaacagt ttccgaaatg ttcgggaaag cgaaaaatgg
    17641 tgtttctaaa gccgcagaag gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagc
    17701 tgaagatctt acagaaaaag ccaaagacac cttagaagat aaattagacg gcacgagagc
    17761 taaagaattg gctgaagctg tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa
    17821 agcttcagaa gtcaaagaag gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc
    17881 agtgaaagct aatacaacaa ccgaaaaaga aaatcaagaa ggtatagtga ttccttcagt
    17941 caaagatcac caagaagcgt tgcactctaa tgaaaattcg attgaagaga aaaattccat
    18001 agaagaaaga aattcagaag aaggaaaaaa tggacagtta ccaaaaactg atccagaaca
    18061 atatcgctat aaacctaaca gtgaccttga aaatgagaag cgaatttcag atttatcaaa
    18121 agtaaatcaa gaagacaaag cagaaaatga gggacagtta ccaaaaactg atccagaaca
    18181 atatcggtat agatctgtta actccgaaaa tgaaaaacgc atttcagatt taccaaaacg
    18241 aaatcaagaa gtacaaacgg atgaggattc catcgttctg ccaaatgata atggaatcga
    18301 agttggaact gaaaaatcaa cgaaaattgg tttacctaag gcacaaaatt taccaatgaa
    18361 agatagtcgt ttgaatcaat tcaaggataa tgatcaaagt tttgttcatc aatcacctgc
    18421 aggtactgtt caaatgaaac caagtcgttt gcctgaaaat ggaaatctga caactgtttc
    18481 ttacaattta tctgaaagtg gtgagacagg gaatgcgtcc gttcaaaatg ttgtgacaag
    18541 agaagctgca ccaaaaccta ttcaaaacgt tcatacaggt tacaagccag caaatgtggc
    18601 atttactgca ccaacaaaaa taaatgtgac aagagaaaca aaaaacagga acaatgcatt
    18661 gaaagaacaa ctattgaggg atatcaaaca ggatatcaaa ccataaaagg cctacaagtt
    18721 aggccttttt tctatgtatg aaaaggagaa atcatttatg gataaatata gtaacatgaa
    18781 aaaccctcca agatttagtg gagaatggaa gtttttcgct tttttctttt tagatctatt
    18841 aacgcttggt ggctgctttt tttgggcgaa aatggtcaat aatcttgtcc acttaccact
    18901 cttttttgca ctcttgaatt atggcttatg gattgcgata gcgttagtga ttgtttggcg
    18961 accctataat aacccaggtc aacgacaaat ttttgcgatt atcgagtcta ttttttttgc
    19021 ggacaagaac cattatcatg cgttagatcc taaccgaaat tatcatggta cgtataagga
    19081 ggatgaataa atgcgagtca aaggacaaaa ttcagttgaa aggaaatcca ttttgcagtt
    19141 tggaaaacca gaagagcgct ctatcttgga cttatttcca tataaatcct gtacagaaga
    19201 gggagtctta attactagag aagatcattt tcaacgttat tatcgtgtag tttctacaga
    19261 cgtggaagga ttaaatgaac aagagaaact agaacgaatg aatcagttga ccaccgtcat
    19321 gcgtacgtat gtgccaacaa ttaagttagt tagtttgacc acagagacgg atctaacgga
    19381 acaaatcgtg caaaagcggc aattattaga taaaaatcgt atggaacagt ccttaggaag
    19441 aaatctacaa cgattaaaaa aatatgaaaa gaaattagtc gaagaaatcc aggaattgaa
    19501 acgagcagaa caagaacgtc ctgacttaag cttctttttc gtcattgaag gaaagacaat
    19561 caaggattta caaaccagaa accgtcagtt aatgcgatct agtggcattt tagggttaaa
    19621 accactctac aaaaaagaat tgatcaagat tctgtatcga atgaacaata tgaatgatga
    19681 gtaggaatgg agaagagaga atgaaatatc cgttgatatg gttagaaaaa gatgttccga
    19741 aggaagagac cactagaaaa tttcctcaca ctttaagcct gggtaagttg ggaaaagggc
    19801 aatcttttaa ccaagtaaag aatcaaacaa gaagaggtga ctgtacatgt tagaagcaat
    19861 taaaacagct ttttcaccac aaaatgatca aaaagtactt gatccattaa agcaactagg
    19921 aaatcgggaa atagaaattg taaaatccaa agggtatgat ttagattttt taaaacaagt
    19981 gatgccacaa ggtgggttaa attttgatga agaatatgtg acgtatggta atggttattc
    20041 acgatgtgtg acagtttatc actacacaaa aaatcctacg cttttttggt tggccaatct
    20101 catgaacaat cctcatacag tggccacact cgatgttagc acagatgaaa agcaaaaagt
    20161 gattgatcaa atcaatcgtg ccttagatga attaaatgat cgatcagata atgaacgaaa
    20221 agcgactgac cgaaataaaa gcgcggaaga atacctggaa ctagcggaat atgctagaaa
    20281 attgacttct aatggggaaa tttctaagca agtcaaagtg agagtacatg tttacggaaa
    20341 cacgattcaa gaagtagatg aacgagcgca ggaactgatc gaaaatctca aagggatgga
    20401 ttatcgtgcg gtgacttatc tgcataaaac caaacgagat tggcaatcga tgtttaccat
    20461 gttcgatgaa caagctgaat ggttcggaaa tatcaaaggg caatcactac catcgttaaa
    20521 cattggcgga ggtttcccgt tccaccacca aacgctaaaa gatccaggag ggacacaata
    20581 tggggaaacc atgacaggcg gcccatttgt ctggaatgcg acgtataaaa atagtgtacg
    20641 gttgtctact aataccttgc ttctaggaat gatgggagct ggtaaatcga ccgttcttaa
    20701 aatgatcgcg gaagcccatt tagctgcagg agactttatt tggggctttg aaaagggaaa
    20761 agactttatt cctttcttaa aggaatataa cggtattatg gtgcgtttag acggatctga
    20821 cggaatgatc aatcctttag agatatttgc cacacggacg tatgatgaag catccagttt
    20881 atatgacgat ggatcagtca aagatttgaa gatcaatgaa gcagcgtcct atcaaacaca
    20941 cttagacaaa gtggtctatc aggtacagtt agtgagtcct cagctaaaag gaacgatgaa
    21001 agcagaattt aagacctact taaatcgttt ttatgaagag tatggaacag taccacgcgg
    21061 ctttacttcc tcaaattcgc gttcaaatac tgatacgcaa gtcacaggga aagatccaga
    21121 agcttatccg acgttcaaag aatttttaga ctttttagga caactggaat taccaggtgc
    21181 ttcccaagag aaaaagaatc gaaaagaaga aatggaatcc atcgtagaat ccttatgtga
    21241 gacgtatggc atgatttttg atggtcatag tacgattcga caccttgacc aacaacagtt
    21301 ggtctttttt gacattgact ctattacagg cttagatcga ggggtacaac aatgtcaaat
    21361 gtacatggct atgacgttga tttggaatca ggcattgatt caaggaagaa gacaacgacg
    21421 tttaattaaa gaaaaacaaa tggaagaaca agacttcaaa cgcttcttgg cgatgattga
    21481 tgagtgccat aatggtgcga cctgttctgc agtgaaaagc tgtgggttgc aagtagtcta
    21541 aaaactattg taataactct tataacgaga agtcaaatga gtgggtaacg ccacaaaggg
    21601 cttccactga tactccgaca tgcaacaagt agtttatgca gaccaacgtt gtgtgaagct
    21661 cggtgaagtc ggctgaagga taccgtaagg taaaagaaat agtgaatacc acgaaagttc
    21721 tctagaggta aagggtgtat ccgataagtc gggtgtccaa aaaaacttaa tatggtgagt
    21781 atgtacaaac gtactgacga actagcgaat gtactgtccg aaaggcatag ctcctagaaa
    21841 tgggagagac agtgagcaaa aaatcactca ttgtgctaac ttgtggttga gtaaaagtcg
    21901 ttgttatgaa agaccatgct gcgttacagg cgtaggagaa gttagggggc aaaagctagc
    21961 atctaagtta aggcatgtgg atagatataa gggaacaggg gaagccatca acatggagag
    22021 tgcatactcg atgaagtggt agtaagaaat atgtgaatga caaataactt actttagtga
    22081 tggtgaaagt agagtcacga ctgatgaagg ttctgtaatg gaactggagg aacaggctcg
    22141 attcgtctgt aaaagtcatg aaacccgttc aaccatggga ttctcgtatg actaaaaagg
    22201 tcacgagcca attggagcgt gataaaccat gacaaaagaa acaagtccaa aagataggaa
    22261 gttgcggcat gcagaatatt atggcatgac ggaaatcttc gataagatat actccaaaag
    22321 tttgaaggga gataatttca agaatctaat gcagattatc atttctgata acaacattgt
    22381 cctagcatat cgtaatatca agagaaatca cggtagtgtg acaagaggag aagatggagt
    22441 aacgattaaa gatattgaaa atctcaaccg taatgaattc gtagaaattg ttaggaagcg
    22501 attcgcacat tataaccctc ggaaagtaag gcgtgtagaa attcctaaat ataatggtaa
    22561 attaagaccg ttgggtattc catcaatatg ggatagaatc gcgcagcaat gcatcttaca
    22621 agtattagaa ccaatatgtg aagcaaaatt caataatcac agctatggtt tccgcccaaa
    22681 tagatcagca gaacacgcaa tagcagattg tatggcccga atgaatcaaa gtcacatgga
    22741 atatgtggta gatgtagata tcaaaggttt cttcgacgaa gtcaatcata ctaaactcat
    22801 gagacaaatc tggtctatgg gtatacgaga caaacagctt ctcttaatca ttaggaaaat
    22861 gttaagagca cccgtagtac ttccaaatgg gaagttgata tacccaacaa aaggaacacc
    22921 acaaggcgga atacttagcc cattacttgc gaacatcaat ctcaacgaat ttgactggtg
    22981 gatagcaaat cagtgggagg aaaaagatgc caaagagcta aaacatcatt ttaggaaaaa
    23041 tggagcttgg gataaaggta atatctatca caagctcaga aagagtacaa ctctaaaaga
    23101 aatataccta gtgagatacg cagatgattt taaaattttc tgtagaaatt ataatgaagc
    23161 gaaaaaagtt ttctatgcaa ctaaaatgtg gttacaggag cgtctaaagt taccaatatc
    23221 tgaagaaaag tcaaaaatca caaatctttc aaaagaagaa agtgaatttt taggctttac
    23281 gatgaaatta gtgaccaaag gtaacaaaaa ggttgttaat acgcatatag gtacaaaagc
    23341 acaaagcatg attaaagtga aactaaaaaa acaaatcaaa cgaattcaaa aaagtcctaa
    23401 tagcaaagat acctttatct atattggagt ctataacagt atggttattg ggattcataa
    23461 ttacttcaaa attgcgactc attgtagtgt agcaatgcga aaaattgcaa gagaactcag
    23521 accaagtttt gaaatacgac tacgagaaca aggaatctca aaagaaggac aatacggaaa
    23581 gtacgaagga tacaagcctt acctacggtc aggacaaata cgatatataa gagggtttcc
    23641 aatattacca gtagggtata ttcaacacag acatccgatg tgtaaaaaaa gaaatattaa
    23701 taaatacacc gaagaaggta gggaagccat tcataagaag caacaaaccg tagaagaatg
    23761 gaagatatcg tggttgtgta gcaatccagt gcttagtaag agagcaacga ttgaatttgc
    23821 ggacaatcga atttcaaaat tcatcgctca aaagggaaga tgtgcagtaa caggtgagga
    23881 gcttatctta tctgaaatgc attgtcatca tattatccct tatcatgaaa gcaaaagtga
    23941 tagctatgaa aacctagtga ttgttactga ggaggttcat cgggtaatac acgctacaca
    24001 aagtgaaacg attgaggaac ttctaaaata tctaaaatta aatccaaaac aaaaagaaaa
    24061 actcaatgag ttgagattaa aagttggtaa cgaagaaata tcttaataaa tatttgtagg
    24121 acgggaaaac agaatgtctt ttatagacga gggaacgccg tatgcggtga aagtcgcacg
    24181 tacggtgtgg actgggggaa aagacggaga taatatcaaa ctcttaccta tcagtatcga
    24241 ttaatccaga ttttattgaa acggtaaatt atgtcacaaa ctttcaacga gaaatgcgta
    24301 aagtagaagc aatgactgtt ttagctacac aatccccaca agaaatggtg ccagaaaata
    24361 tgtcttctga taacttctcg aaactgaaga aagtctttga attttcttct acgaagatct
    24421 ttatgcgtat ggatgaatct attttacgtc atatagaagg attagtgggt aaatcaatga
    24481 cgaactcaga attagaatcc attcctcaac aaaatcaagg agacgctgtg atcaattttg
    24541 gctcaaaaga aaccattcgt gttcatttta gtccgaacaa acgtcaatta aaattgtttg
    24601 atggaggaaa ataatggaaa accaaaatga aagtttaatc aagcagtatg ttaagcgtcg
    24661 tgcaaagcgg cgcttatttt tgtggttgtt tggtacttct gcaggtttga ttactatttt
    24721 gatcactgtg ttcgtgaccc tatttctaat acttgcggcg ggctcgattg ataattcgga
    24781 ttcagattca agcagtggtg gcgaagcttt tacaggagaa tattcggaag gcttaccgat
    24841 ttataaagaa atcaaaggaa gaggcccttt ttctgatgag attgcgcaat atgccgtagg
    24901 tgcagcagtg aaatataaat tattaccaag tgtgatcctt tcgcaatacg gttacgaatc
    24961 agcgtttggt acatcggcgt ccgcaagaaa tgatctcaac tattttggga ttacttggtt
    25021 tgatggttgt ctgtttccta aaggaactgc acgaggaatt ggtggcattg aaggtggctg
    25081 gtacatgaag tttcccaact caaaagctgc attttcctat tacggcttta tggtagccac
    25141 acaatccaat tttaatgctt gtgtaggaaa taaaagccct ggcgcaagct tattgatctt
    25201 aggacgaggc ggttatgccg ctgcaggaat tacggaagat agtccgtatt atacaggttg
    25261 tatgtcaatt atcacttcta ataaattaac cgaatatgat gaattcgcca tcaaacattg
    25321 gggtgaaggt ggcaataaca acggcacgat tactggtgaa tggacgaatc cattccctgg
    25381 cagcagttta gataaaagtt ccttcagcgg tggtcaatta tttggaacga atccaggcgg
    25441 tgaattccgt ccgaacggct tccatgatgg attagatttt ggttcagttg atcatccagg
    25501 aagtgagatt catgcggttc atggcggaaa agtcgtgtac gttggcaatc ctggtatttc
    25561 aggtttgggt gcgtgtgtga tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga
    25621 atttgcaaat agtacaggaa attctcgagt gaaagtaggc gatcaagtga aagttggtca
    25681 agtaattggg atacgagata cagcacactt gcatttagga tttactcgaa tggattggcg
    25741 tcaagcacaa ggacatgcat tcattgatga tggtacatgg atcgatccgt taccattttt
    25801 gaatagcagt aaaaaataga tcaggagaaa aaatatggaa aataaaacgt taaaaatcgt
    25861 cttaacagct gcaattagcg ttagtttaat cggcaatatt gcgttgtttt ggcagctgaa
    25921 agaggaacaa gcagcaaata cctataaaat tgaagaagtg gctaaaaacg cgtctgagaa
    25981 gctcaaaaaa aattcctctg aggcacaggc gaagatcgac aagttaaatc aaaccatcaa
    26041 tgagaaaaac caagaaatcg atcagctgaa gcaacaagat ggcacaaaag caaaagagga
    26101 agtaactgaa gcacaaagaa agacggcaga agagttcggt caattggcca ttgataaatc
    26161 attaagcaga aatgaattaa cggaaaaact aaaagatgtc gcaacccaag aagtgatcga
    26221 caaattaagt cctacagacg atgatcacca acatgatgat tacggaagtt actcctttac
    26281 attaggtgac gtgcaaactt acgctcaaac aagcagtgta aaagaggaac aaaattttgt
    26341 cgtatttgtt gattatacaa tcggcaatcc gcaattcaaa gatgtcaaac cacaaaaaat
    26401 aaaaggtgga ttaactgtca ctgaaaaaca agtagatggt caatggaaag tcatagactt
    26461 tagttatttc actcgttagg aggaagaaaa tgagtaagaa aaaaggagaa agacaacgtt
    26521 tcaaaaaacg aaaaagttat cttagatggc tgggaatctt tatgattctc ggctttttga
    26581 ttcttgctag tccgatttat acgaatcaga aggtagatat accagatgag gtaagcacga
    26641 acgccttttt aaacacgaaa gagctgacaa tggtttcaaa agaaaaagga gcgaaccagc
    26701 aattagtttt tgaatttgcg atcgatgatg aagatcaaag cttgttaaaa gaactggcta
    26761 atcttaatta tcgcgtagaa gtaaagacag ctaaagggga ttatcaagca atacgagcca
    26821 aagtgattaa ggtgtctgat gattattttg cagtgaagct aacgcatgta ccagaagaat
    26881 atatcgccat gcgtttaacc attattcctg aaaaaattga tccaaaagtg gatatgcaag
    26941 aaccacaaga tctgattttt tatattcacg aagataaggt caaagatcac gtgaaagacg
    27001 aagattatga acaacatgcg atcaattaca aagtgaaagg atacaagaaa gaacaaaaag
    27061 cagctcaaaa acaaatggaa tctttccaag caaccattga tttgaatgaa gaattagtga
    27121 agaagctaaa agatcaattg ccttatcaag tcgcagatga tcaggaaaat actgaaaaca
    27181 agattaatag ctaccagcaa gaaattgaaa catcgaaaac gcaactgaaa gatcaagaag
    27241 agatcattca aaaacttcaa gaaaaaattg atcgtatgac aaaagaaaag atttagaagg
    27301 acaggtggag tgaaaaaatg aagcgaacaa acttggctga caaagatact ttcgctgagc
    27361 agaaagtatt gattaaagcc tatctcaaag ttgtaaaagc taaagaaata tgtcgagaag
    27421 tgaatttatc taacacaaca atatctgagt tgaagaatgg tcgaaagaat ttagatacct
    27481 taaaattcag atcatttgaa gctctatatg tagctgctaa aaaatatttt acagctgata
    27541 acaaataaaa aacaggagta ttttatgtga aaaaagtata taaaaaatgc ttatgaatca
    27601 gctatatcac cttaaccatt tgttaatgag atgggagaaa acattaacca cgattctaac
    27661 gcaaacatca agcacataaa acatttaaca ctcctccttt ctggctgaag atttcaacca
    27721 aacaggaaga cagcgtatca attatagcat acactttttc ttaaattcat ttttcatatg
    27781 acaaaatatt tccttctaat cgacaaagtt agaaggattt tttattgtct aaatacacaa
    27841 ataaccttaa tttttaaaga gcataaggaa acgtgatatc actttctcac gaaaggtgtt
    27901 aggtaggctg tgcctaccac gtttctaaga aagataaatg atatcaaaat tttggatttg
    27961 tgtgtagatt tgtgggtagg atgaaagcga atgtgggcag taaaataagc gtttgtgtgt
    28021 agcaaaatca aaaaatgtgt agggatatgt gtagctacac atatccctac acataataaa
    28081 tttggaggaa aatatggact tattaattag aaatattgaa gaaaagtata ttaataaaat
    28141 tgacaaacgt tgtgaagaac ttacggtcaa gactggcaaa aaatggagtc gaaatcaata
    28201 cctaaaagtt ttgatcgaaa atgattttga tcacgctctt ctgaattata aaaaagacca
    28261 atttgatcgg ttgttagaaa aatttgtgga tattcaaact tataatacga aaactttaga
    28321 agaatatatt gcaacaaata atcaattaat tggtttgtta attgaataat aaaaatagta
    28381 ttgtgaattt ataaaaggag gaaatcaatt ttggcaaaag caacaagaaa acatgtatat
    28441 ctttacgata tgcatatcga agtacttgat gaaataattt ctactcatga agatatatct
    28501 aattatagtg aagcagtcag acatttgtgt atgatgtatg atccaaaaat cgaagaaaaa
    28561 ataaatccaa ttcaaattaa attgaatgca atgggaaaag aaatttctat tttaaccgaa
    28621 atggtttcag agtttgcgga tcttcatttg aaagatacag gaatactaat tggtacaaat
    28681 tcaactgttt atcaagaagc agaagaacgt gtggaaggaa aaattaaatc caatcaaacc
    28741 aaaaaatatt cttctaagtt aaagaaacca gtgaatgaat ctgttcatac cagagtagat
    28801 ccatttgatc caacgaagat tctaaaaaga taaaagtaga aggagaacgt gatgaataaa
    28861 tcgcctgcag ttattcttac ttgtcaattt accttgccca ataaaaaatc tttttcaaca
    28921 tacattgatt atatgacaag agaaaaagca ctggaagaaa tggaacgacg tacgcccgaa
    28981 gaagaacagg aactgcaacg aatcaagaat gctttagagg atttttatat tcctgaagga
    29041 gaaacttttt cggaaagtaa gacggataaa gaattcagtg aaacgtcgaa tgaagctcaa
    29101 tcgttgatgg aacatggaat caattttgac aagttagagg aacaagattt taccaaatat
    29161 ctttcttata tgacccgtca ctatgcgtta gaacaaaaga aagaactgac gcaaagggaa
    29221 caaaaagaat accaacggtt gaaccaagca atgaaaaaat atcaaacaga gccacataaa
    29281 aaagcttcag gaaatcttcc tggagttttt tctatggctt cagacgaagt gaaaggaagt
    29341 gatctgaagg agatcaaaca aatcttccaa agaggacaac aaaaaggctc aatcatttat
    29401 caagatgtta tctctcacga caattcgtat cttgaaaaaa tgggcttgta cgatcccaaa
    29461 acagatacct tagatgaaga aggtttgaaa gcagctggaa gaaaaatgat ggagacatta
    29521 ttcgagaaag aacaattgaa tgaaacaggt tactggatgg caacgattca ccgtaacact
    29581 aagcatatcc atattcattt tgcagttgtt gaaaaagaaa atacgcgtaa accgtatact
    29641 gagattgaaa atggtgtgag ttatatcgta ccgaagggga agagaagaca agcaacatta
    29701 gacgcaatga aaaccaatta tgtacatgag ttagaacgtt tctcttatga aaaggatcga
    29761 atgatcggta tggagaaacg aaatttattg acacgaaaaa gtgatttgcg taacacctta
    29821 acaaaagtag taaaagaacc agcacgttac gatcgacacg ccatgaactt attaaatgaa
    29881 gtatataaaa ccctacctga aaaacgttcg gaatggaatt atggagatga aaatcgaacc
    29941 aagctttcac catttacaag gggaaaactt gatgagttaa ccaaatatgt tttaacaaaa
    30001 gaacctggat atcaagaata tatacagatt tcaaaagcat tgaaagaaga agccaaaagc
    30061 ctttatgggg aaagcaaaag agaaacaaag aatgcagaaa aaaacgccct ctttgattta
    30121 aaaaagcgca caggaaatgc gattttaaca gaactgaaga gacaggatag tcaaataaaa
    30181 ccattaatag aaagtgtcaa tcagatgcaa tctatttaca atgaacgatc ctttcgaaca
    30241 ctcaaacagt tgccaacaaa tgaatacaag tatagttatc aagaattcgt aaagcgtagg
    30301 aaacaattcc aacaaagagt tgtcacgcag cgaagcttgc gtcaattaaa tcgaacaatt
    30361 aatgcacatg aagaaaaata ccacgcaagt aaagcatatg aagagcaaca gcgaagaatc
    30421 caaatggaac aagaaagaca ataggaggaa gagaaatgaa acaatattta gatcaatgga
    30481 aagtcataga aggatcacta aaagaagaac gaatcgaaca acttcctgat tgtttagaaa
    30541 aagagcattt gtttcagata agcgaaatgt tgagaaacga acagtttgat ccaaatcaat
    30601 ttttagttgt tgagtaccca gctactggtg tgtattgttg taatcatgtg aagggtgaaa
    30661 aatattttat tattcaagaa tacgaaggta agttagcgcc ttattatact acttgggaaa
    30721 taaatgaaga gggaatcaat aatttcccat gtgaaagcat tgaagagtct atttctctca
    30781 cggaatgtta aataaataga gacgtaaaag agtcctgaac gtgattgtcc aggactcttt
    30841 ttcaatgaat tgttttccgc caattcactg agtggtgcga ttcaatttta ttatatcaaa
    30901 agaaagtgag attccaaagt gaagggagga agaagaaatg actcagacaa cgattggtgt
    30961 tgtggttgaa aaaagcaggg ataatctcgt ttttgtgaca gaaattcaaa caggtagagc
    31021 ttttgtagtc acagacaaag ctgcaaaagc gtatcaaaat ggcaatattt taacgttaaa
    31081 tctgactacg aagatgttcg ttgatgcagt agaaaattat ccatttgttt agacaaacga
    31141 tattttacaa ggaggaaacg gacatggcag aagaaaaaga accactagat aatagtcggt
    31201 tgggaaagtc caaaagaaaa ttagtacggt tgcaaaacga attaaataat caatttcaaa
    31261 agctatttga tcaccaaaaa ttgacaaatg ggcaaccaat gaatgacaaa cgaaatggtc
    31321 gttcatggtt taaccagcaa gaaaaaatag aaaataaaat acaaagtttg agagaagaga
    31381 tcaagcaaca agaaaaacag gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg
    31441 gctataacaa gtacggcgga ctggatatga cgatcgataa tattccaaga atcaaagaag
    31501 aaatcgaacg ctttgaaaaa ggtgaatcga ctttttcagc cgccacgatc cgaaaatatc
    31561 aacggaagtt ggaaacatta gaacagctga aagaaaaatc agaaaaagga aaagaaaata
    31621 tcctccctga agtacaagcg atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc
    31681 caaccattta ttttttgaaa ggctatcgaa aagttgcttt agaactgaat gaaaacgggg
    31741 gatttgagga atcaaaaaaa taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg
    31801 aattacttga agaggctgca ccaactgatt ctctgaagga gaaagaaaaa gatcatttct
    31861 atcgagtaga attcaacgaa aatgatccag aaaaaggatt gaaaagttat ggtggggaga
    31921 tcgtaacatt agaattgatc gatcaactta aaacgttgga taaccaatac gctgcagaag
    31981 caggttatta taaattctat tttgaagaag tgaagaacgg agaagtagta gatcagatcc
    32041 gaatggatct aggggatggc gtcaacagta atcaccccat ctatttagag ttagcaaaat
    32101 tgtgcgaaca agtacctgag aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga
    32161 aagaacaaat gcagtcgaaa gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg
    32221 cagaagagat caaacaagaa gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa
    32281 agcataccca ttcttctgaa gacgtcaaag aacttttgaa ctttatgtct cgttttccag
    32341 aacgatcctt tagaaatcaa ttattgattg aggaacaatt tccaggtgca acggcttgtt
    32401 taggtcgtgc cgcgttgaaa aaagaaggaa tctatatcaa aaaaggggaa caagggaaca
    32461 agatctttgt cagaaaaacg gtcaaaggat tttatgagaa aggacgtggc tttgttcgag
    32521 aaacagaagc tacaccagaa gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca
    32581 aaaaacctta ctacacgatt gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg
    32641 aagactatcc gaaaatcttt ccgaaccgtg tctttgattt tcaattggat aaaaaaggac
    32701 agtcagaact tcaagctggg attcaagcgg tagccaacaa tattggaata caaattcgag
    32761 atatgacaga aagtgaagtc tatcaaagag aattaggaca agcacgtggg gcgtatgttc
    32821 gtaacgaatt tactggcgaa gaagaaatcg tcatgaatac tcgaaatact cctacgcagc
    32881 atttagctac aagtattcat gaattagccc atgcaagaat gcacaaattt tcagaattag
    32941 ataccgcaac taaagaattg caggcagaaa tgacttctta tattgtgtgc aaacattttg
    33001 gaatggacac ttcggagaaa gctattcctt atattgcagc atggacgaag aatggtcaga
    33061 cgttggatga taaggaagcg gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg
    33121 caaatgaatt cattcaaacc atttctaatg aaatcaatca atacagagaa agagaaccag
    33181 agatccaacc agcagaagtg aagcaggagc ataaaaatac tgtagaaacg gcagagaata
    33241 agaatattgt gcccgttggt tcactttgga tcgacaagaa agacgggaaa gtaatggaag
    33301 aagatacggg tcgaacgtta catttgaagg atgcggcgtt tattgaagat ccgcagcata
    33361 atcaagtgat tttaaaagga aaaagttatg gaaaagagat tcaaacggcg ttaccttcag
    33421 aagaatttgc gctaggaaat ccattccgat tttcacttga aacagtgtca gaagtggcca
    33481 aacgaaaaaa tgaaatctat tatgaacaac aattgtcaca aaaagaacgc gtataaaaca
    33541 agtaaaaaag actatctgaa aaacggatag tcttttttct tttgggaggt ttgaaaatga
    33601 ccgaaaaaaa aatgagtctt attgatcgat gtaaacagat cgatatagtg gattttgctc
    33661 gaaacaatgg aatggctgta gtcaataaag gacgagacta tcgcttagaa gaccatgatt
    33721 catttgtttt tgatcgaaga aaacaacgtt tttattggaa cagtcaaaat attagtggcg
    33781 acattattga attagctaaa ctttttttca tagacaaaga gatccaagac tctaagcaac
    33841 aatttaaagc agccactgat tttattctaa agaatgaaga caaaacagaa cgagtggaaa
    33901 atcttcattt tgaaacagaa aagtacaaag atcaccccgt tgattatcaa ccactcacag
    33961 aaaaaggaag gaattatctg aaggaagaac gaaaactacc agagtggctg attgattatg
    34021 cagaaaaaga agggttgatt gccgaactaa agccaaaata tgaacggcaa aatttcttag
    34081 ttcgagacga tcgtttagat catgcggtcg cctttttatg gaaagatcca cagacaaaag
    34141 agacagtagg ggcgagttat caaggaacgt tcattgatta tgaacgtttt ggtgagagag
    34201 gcacatataa gcatatagat aaaaattcga cagcgaatca tggctttaac ctaaagatcg
    34261 gtgaccctaa gcagttaaaa ttttttgaga gtagtattga tttacttagc tatgctgcac
    34321 tgaatcgtga tcagttaaac gatacgtggt tagtctctat ggaagggtta aaacaccatg
    34381 tgatcagtca ttattttggt gaggcagtat ctgaattgag aaagaaacaa gcctttcctc
    34441 aatccatcga gatttgtgtg gataatgacc gtgcaggaca tattttctac gaaaaagaac
    34501 aattaatggg cgcagtcgat ccatttacga atcagaaagt acgttgtgaa cgaggaatcg
    34561 ctaatgattg gcaagtgcca aaagaataca aaatcattta tgaagaagtc gcaaaagaag
    34621 aaaaggtcac accagaagct attatggcca tacacaagac agaaaacaat ctgcaattaa
    34681 cgaaccaact ggtttccgct cacaaagtaa atgcgttttt tggccagcag ctttctgtta
    34741 acgattcaat agaagccatt aatttgaaag atatttgtag agaagtcgca aaagaattaa
    34801 aagtttgtga acgcgtggat ggtacgtacg attttgatcg tttttatcaa gaaaaagggg
    34861 atattaatgc gcagattctt ttctcttata aagcagaaca atactacaaa ggctataaaa
    34921 atcatgaaca tgaatttata cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg
    34981 agatccagca acaagaaatc agaaagcaaa aacgtgccat gttgtttcaa caaggcagac
    35041 aacaagaaag ggagtgaaat cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca
    35101 caagcgaaag cttatgccga tagcttttct aaagctaccc gaaaagatgg gtattttgag
    35161 atccaagatc gattgtttac aggagaaact gtgatcacgt atggttttgg tcatttagtc
    35221 gagttagatt cgcccgatat gtacgatgaa aaatggaaac aatgggcatt agaacatttg
    35281 cctattttcc ccaatcaata ccactatcat gttcctaaag ataagaaaaa gcagtttagt
    35341 gtcgtcaaac gacagctgca gtcagctgat acgattgtga tcgcgactga tagcgatcgt
    35401 gaaggggagc tgattgcctg gtcaatcatt caacaagcag gcgccaatca aggaaaaacc
    35461 tttaaaagac tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccaacag
    35521 ttacgagacg cagaagagac ctatccaaaa tttgaagaag tacaagcacg tcagatcgcc
    35581 gattggttaa ttgggatgaa cggtagccca ctgtatagtt tactcttgca gcaaaaaggc
    35641 ataccaggaa gtttttcttt aggtagagtc caaaccccaa cactctatat gatttatcaa
    35701 ttgcaggaaa agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta
    35761 atcgctcaaa atggcgcatt tgatgcgaag cttgatccaa atgaaaccca agcgacacaa
    35821 gaagcatttg aagcctattt aaaagaaaaa ggtgttcaat taggaaaaca gccaggtacg
    35881 atccttcaag tcgaaacaga gaaaaaaagt gcggctagcc cacgtctttt ttctttatcg
    35941 agccttcagt ctaaaatgaa tcaactcatg aaagctagtg ccaaagatac tttggaagcc
    36001 atgcaagggt tgtatgaagg aaaatatcta agctatccgc gaacggatac cccttatatt
    36061 acggaagggg aatatgcgta tctgttggat cacttagacg aatacaagca ttttttaaaa
    36121 gccgaagcga ttccgactcc tattcataca ccgaatagtc ggtatgtgaa taataagaaa
    36181 gtccaggaac attacgcgat cattcctaca aaaacggtta tgacagcagc ggcttttgaa
    36241 caactaagtc cactgcagca ggctatttat gaacaagtgt taaaaacaac ggtggccatg
    36301 tttgcggaaa agtataccta tgaagaaaca acgatcttga cgcaagttca gcagcttcag
    36361 ttaaaggcaa tcgaaaaggt gccgctagat ctaggttgga agaagttatt tggaaaagag
    36421 agcgacggaa aagagaagga agaagagccg ctacttccta aagtgacaaa aggagaaatg
    36481 gtgacggtgg atctacaagt tcttgaaaaa gaaaccaagc caccacagcc ttataccgaa
    36541 ggtacgttga tcacggcgat gaaaacagct gggaaaaccg ttgatagtga agaagcgcaa
    36601 tccatattga aagaagtgga aggaatcggt acagaagcga cacgggcgaa tattattgaa
    36661 accttgaaac aaaaggaata tatcaaggta gagaaaaata agctcgtggt gacgaataaa
    36721 gggattttgc tttgccaagc cgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc
    36781 gccaaatggg aaagctactt gttaaagatc ggtgaacgaa aaggaacaca aaccactttt
    36841 ctagccaata tccaaaaatt tgtctctcac ttattggaag tcgtaccagg gcaaatccaa
    36901 tccactgatt ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc
    36961 gcgcgtcatt taggaatttg tccaaaatgc caggaacaag aagtcttact ctatcaaaag
    37021 gtggcagcat gtacgtctga agcctgtgat ttcaaactat ggacaacgat tgccaaaaag
    37081 aaacttacag cgacacagct gaaagagatc attcaaaata gccgaacaag ccaaccagtg
    37141 aaagggttaa aaggtcagaa gggttcgttt gaagcaacga ttgttttaaa agaagacttc
    37201 actacaggct ttgaattttc tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc
    37261 actacaaaat aatggagggt taatcttatg cgtaatacaa cacacaaaca acaagaaatc
    37321 gaaaatcaaa aagccaaaat tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga
    37381 ctgcagcaat tgaagaacca gcagcggatt caaaaagaaa tcgaattaga tcgataaatt
    37441 cctttcttgt taatataaaa ctaacatgtt atagttgtgt tagttttata ttaactttag
    37501 gggaagagta atgaaagaca aaatccaatg tatttttgat aaacataatg gcaacctatc
    37561 cttgaaggat gctagaagtg aaggattatc gcctatgacg ttagatcgat tagccgaaaa
    37621 ggataaaata gaaaaggtag caccaggttt ctatacctta aaggatgcgt tcgtggatga
    37681 attgtatctc gctcagagta tctattccag agggattttt tctcatgaga ctgcgctcga
    37741 tctttttcaa gtaggaacat atatccctaa aaagatcaat atgatgtttc ctaaaggata
    37801 caatgtatca aaggagaaat tagatcagta tgccattcaa ccgcattaca ctaataaaga
    37861 aaattttgac ctgggagtca cagaaacaga aagcttttac ggcaataaaa ttttagtcta
    37921 tgatttagag cgaacgttat gtgacatgtg gaatccaaga tacaaagcaa gcgtagaggt
    37981 aaaacaagaa gcattaaaag aatatatgac aagtaccagt agaaatacaa ataagctaag
    38041 aagatacatg gatattttaa aatcaccaaa agaaatgact ttgtatatgt tgccacttta
    38101 ttgagaggag taaagatgaa tgacaccgac acaatttaaa gagaaaacaa gaaatttcgc
    38161 agcagagaaa catttaaacc cacaaattgt ccaaagatat tatatgatgg aaaaattatt
    38221 agaatgtatt gttgaatcgc cttatcatga tgattttgta ttaaaagggg gatttctgat
    38281 tggatcaaaa tacgggttag aaaaccgttc aaccgttgat attgatacga cttatcgaaa
    38341 ttcgaaacta acaaaagaaa agttagaaac tgttttttct gaattaacag ctaatcctac
    38401 aaaagatgga attcgttttg aattaaaggg attaacagag acgagggaag ccgattatta
    38461 tccaggtttt caagcaaagt ttattgctta tcttgaaaat gcacgtgtac cttttaaatt
    38521 agatattact tcaggtgatt ctattattcc agcagcattg atgtatgaac acaaattaat
    38581 gtttgaagat aaaacgattc atattccagc ttatcctacg gaacaaattc tagctgaaaa
    38641 attacatgcc acgtttagtt ttggaaaaga taattcgcgc atgaaagatt tttatgacct
    38701 gtatatgatt ccgaaattag aacgtatcga tgagaaaaat ttgtataaga gtgttcaagg
    38761 aacattcaag gaaagaaata atcaatcggt gtttaagcat ttgtatgatc aggaaatgcc
    38821 tcaattggtg aaagatgagg gcttgagaga aaagtggaaa agataccaac aagaaaacta
    38881 ttttgccaaa gacatttctt tcgaatctac actaacttcc gttgattccc tgatgcaatc
    38941 aattgtagag caagagaagg cagaacgaaa acaaaatttt aagagatcac aacaaatgaa
    39001 tcaagaaatg gagagataaa gatgatcaat aatgtaactt tagtagggcg attaactaaa
    39061 gatccagatt tacgttatac cgcaagtggc acagcagtgg cgactttttc cctagccgtt
    39121 aatcgtaatt tcacgaatca aaacggcaat cgagaagctg attttatcaa ttgcgtgatt
    39181 tggcgcaaac cagcagaaac gatggcgaca ttagctaaaa aaggcagctt gatcggcgtt
    39241 gtcggacgaa ttcaaacacg gacttatgac aaccagcaag gacaacgtgt gtatgtgaca
    39301 gaagttgtgg ccgataactt ccaactatta gaaagtaaag cagcgacaga aagccgtatg
    39361 cacgctgatc aatcgtccag cagtccaagc cctaccacct ttgaacaacg ggatacagcg
    39421 acgcctaaca acaatggatt gaacgcttca cagaatccat ttggtggaca atcaatcgat
    39481 attagcgatg atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt
    39541 ttatgttata atttagacaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg
    39601 gcgggctagt ggctttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc
    39661 tttcttggca aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat
    39721 gattgcaaaa acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat
    39781 taggccgggg tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg
    39841 atttttgcta taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata
    39901 aaatccgagt aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg
    39961 aatcgattaa ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg
    40021 atggaaaata caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg
    40081 ctagcatgta tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaataaaag
    40141 aagagttgta gtcctaaatg aggattgcag ctctttttgt tttacataga aacaggaaaa
    40201 gaccttttta ggaagacaca agtgccttct caattgaaaa acctttctct cactccaacc
    40261 gccaccgccg ttcgagaaga aaggaagtga acgctgcacg ttcactaaga tatggtgtct
    40321 tgcaaagcaa gacaaaatag acaaaacttc ctctcaccaa aaggttcgtt cctgttcgtt
    40381 atctctttat tgatttgcca gaccggcaac aagttgcctt ttatcaccaa aaagcgacaa
    40441 ggacaagggt aaatggcacg ctgcgcgccc ttgtccttgt cgcttttcgt tgatttattc
    40501 tggccaacaa atcaataaag aaagaactaa cgaaaggaag taaaaccttt cctaagagga
    40561 ggcagaatca aacatgcgaa agaaatttga ttattggggt gttcccttca gcgaattatt
    40621 cccaaactat catgcaccac atactgttga atgcgattgt ggagaaagag cgaaatgtat
    40681 caaaagctat cgtttgtatc aatgtccaac atgcgggaaa aaatatacct tgtcttatgg
    40741 cgattacatt ttgattgatg agaaaggaaa gaaacgctga tgaaaacaca acacaaatta
    40801 ttgactgtaa aagaagtcgt tcaaattttg gtggacaaag aagtcatttc tgaaaaagta
    40861 gcagaagctt tacttattcc gcggtactgg acgatagaaa cccctttagt agaggatgca
    40921 ccgactatgc tatttgaaga cctagtgaag attgcaggat atgaactgca ttatcaagaa
    40981 aacatggaaa cttatgaaat cgaatattgg ttaaccaaat tagagaacta aaaaggagaa
    41041 aaaatatggc tcaaaattac tatgacgaat ttgtcaaatt acctttggat aaaatggcac
    41101 aaaaaatgga ggatatgacg ttcctatacc atgaaacacg tgtacccaaa aaacattaca
    41161 aggaaaaatt gtcagtcgca gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca
    41221 ttgcgacgta ctatcgaaca ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc
    41281 aagccttact atgcttggaa gtaggtgtca aaccaagcac tatcaaacct tccgaatatc
    41341 aagccttaga actgacttat gcaaaattcg cacagattaa gaaagctaaa gcaatggcta
    41401 gcgactggct agatactttt gaaagtatcg aaaaaaatgg tgcattttat acgattgaat
    41461 cggaggagaa agataatgaa taaggtggtt tatcagctaa caacagaaga tgtttcgcaa
    41521 tttttaacaa gcaaagaaca cttgaatgaa gtaatcagac ggattcaaaa agactttagt
    41581 tgtatggaag aaatgaagta ctttattcaa agagtggaag aacagttgta tatggaaagc
    41641 gaaacaagtc aaacgaaggt cacagtcagc cataatcatt tagacgtgta taaacgtcgt
    41701 cctgaaacgg taaaaaaagg aacagagctt ctacgactag aaacagaaaa atacatcatg
    41761 aatgtgtacc tagaaaggag tgagctggtc gaaaactttg tcagctacac gatttgtagg
    41821 aaacactatt actcagaatc agaatattat tcaattttcg agagcatgga attaatggaa
    41881 gaagaaatgg aagagctaaa cagcaaaaat gcgacggtag acgattatta ccttgtattt
    41941 actaggtttt tgacgtgtgg gacggaatct atcgtgttaa tgggaatgga ttatgaactc
    42001 gctgtacccg aagcattttt gagggaatat gtagaagaag tagataaccc aggcgacgac
    42061 tggtacacga ctttaactga ttttttagac acctacactt acgacgatac agaagaaatc
    42121 caaaatgaac ttgaccgtaa acgaatgcca tatcaacgat ttgaaaggaa aaaaggtaaa
    42181 taaccattta taccgaaaag tgaggatgtg tgaaataaga acaaaaaacc ttgaaattaa
    42241 gggaatggtt cttatttcgc accaatcgct ccaaccgccc ccgccgctta ggtggtgcga
    42301 aataagcggg caaagcccga taacctaaga gaaagaagga gatccatatg aataaaatca
    42361 ttcctctatt gttcgtactg tttgttggcg agatacttat gcgtcatgct gcagctgtac
    42421 atttaaactt cgtagtattc ttaggaatac tggtattttg tttgatcttt tggcgatttg
    42481 tattagccct tgtgagtcta ctattcaaga tgattgcagc catatttttc atagggttat
    42541 ttcgatgaaa ggagagagta ctaatgccaa ttgcaatagg aaataaacgg ttacccgtga
    42601 cattagatga aaagagacaa aaagaattgc agcaactaaa gcagaagtac ggaaaaagtg
    42661 aatctaagat tatgtgtatt gcgttagatc tattgatcgc gcaagaaaaa gcaggatttg
    42721 atataccagc actaaaaaag tgacgtcact tttcatttga agaaacaaaa gtgatagcac
    42781 ttaattgttc ctactattta gaatattttc tatgtccttt ttattccatt ttgtatcaat
    42841 aactatgaaa tctttaaagt atccagaaga gattattttt agatcaattt ttttcttacc
    42901 tgattctaaa gattttgttt tcttaaacat attatgattt cctttcgact attttcatat
    42961 aaatgttaac atagtttatg gattcatgga atgttaatct aatttaatag aaaaggataa
    43021 ttaagtatat acgttttttc ttatgtatgt tatactatgt atatacagaa agaggtgtct
    43081 actatggaaa tgttacctat aaaaaaatgg ggaaatagta atggccttag attgccaaag
    43141 tatattatgg aatatcttgg aattcataca gaagacaaag tgaagataat acaagaagaa
    43201 gtaaacggtc agaagcggct aattatcgaa gcggctaata tagaaaatga attaacaatt
    43261 gaacagttat ttgaaaatta cactgatgaa agaaatcatg tgacgattca aaatttaggt
    43321 gaagcagtag gtaatgaaaa atggtgagaa aacctagaca aggcgatatt ctattattga
    43381 atacagcacc tcgatctgga cacgaacaga caggtaaaag accttatatt gttttaagtc
    43441 atgatattat tgcggattat agtaacgttg ttacagttgc tccaatttct agtacaacta
    43501 gaaactatcc gctatatgta aatataaact cagaatatga aatgaagaca acaggaaaag
    43561 tattattaga tcaactaacg acaattgatt atgaagccag agagtgtatt tttttggaaa
    43621 aagcgcatga tagtttagtt gaggagttat tgatgaaagt tagaacagtt tttcaaaaag
    43681 taagcaaata aaatagaaac agcagcatga tcaactattc gtgattgacc atgctgtctt
    43741 tttttattct atatacggtt tgtctcgtaa cattgtagtc ttttgcaatc ttagcgatcg
    43801 caacaccttt ttttagatcc tcaacgatgt ttttataaac aagacgacgt tggggatctt
    43861 ttgcattggc actgtatagc ttaggacgtc ctttataaac accgttcgct tttgcaatct
    43921 taatgccttg tgcctgacgt cgtttagatt cggtacgttc ttgttcagcg atcatggcca
    43981 aaatttgaat gatcaaatct ttaatgaatt tatccaggag aggattgcca attgcttcgg
    44041 ccatgatcgg tagacttgtg atgatcagct gcacattttt cttcttcaag taattgactg
    44101 aagcgatgat ctcgtcgtaa tttcttccaa gtcgatcaat agcttccaca ataaaaatat
    44161 cttgatctcg gacgaaatcc agtgcttctt gaaaaacaag tcgatgagta atcgttgcgc
    44221 cagattgttt ctcaatgaaa attctctctg caccgaattt tttcatttct tcaatctgcc
    44281 gttgttcatt ttgatcgatg gatgagacac gtatgtatgc aactctcaaa atttttacct
    44341 actttctgca actatttaaa aaacacccta atcatacacg agaaaaccag atttttcaat
    44401 gtcgcggtcg tgtctagtta gggtatacct tatatagaca aaaactaatg atggaggaaa
    44461 tagtatgtgt cacttttctt tttaaatggc aatatttttt gtaaataaat gtcaatcaag
    44521 aaaatttaaa cgattatgac attaaaaata tattttatga attttctagt atataacttg
    44581 atttatctgt taagatttat taagaaaacg ttttcttatt tttgatttcg aggtgataga
    44641 caatgaaaaa taagtttatt ttatctttag gtattttaac tattggaatt agtgtaactg
    44701 gaactacaat tcctacattt gctgatagta caaatgaacc aactactgta gtagataata
    44761 accaagaatt aactccttta gataggaacg gaatagccat tggtaaccca actaacgact
    44821 taactgttac tgaaaattat ttaatagttt ctccattact tgctcgagca gcttctaaaa
    44881 cgatacgagt gacaggaacg atgatttttg ataaagttgg ttggccttct agacctcctc
    44941 aatcacggaa ttggaaagaa aaaagaaatg gttattggta ctatggaaaa ttgtatttac
    45001 aaagttataa atcagctgga aacaattgga ttgcgactta ttcaggaacc ttaactagaa
    45061 agtagggatt ttttatgcca gaatttgtga acacaccaaa tttttatcaa gatagcgata
    45121 ttctaagtaa aattaattta ccgaaagaag aatcagcttt acaagttcag cgaactttac
    45181 aggagacaca aaaaattcaa aaatcaattt ctaatctaga agattctgaa gaaaaaagaa
    45241 aaaaaagaga ccaaaaaaca tatcaaaaag aaaaataatg attttaaaaa aagagtgtct
    45301 agtcgacatt ctttttttct aattgaaagt gttcataaaa tcacccattt ctggacactt
    45361 tctttttttg ctttattttt caaaaaaaca attcatttca tgttcataaa cccataacaa
    45421 tatctatgtt cagttaaact atatggataa agttatgtta caataaaatt aatgaaggag
    45481 tgattttttt gaaaatagga tacgcccggg tatctactgg tttacaaaat ttggatttac
    45541 aagaagacag cctaaataaa tttggttgtg aaaaaatttt tactgatcat atgagtgggg
    45601 caaaaagtaa tcgaccagga ttagaaatgg ccattgattt tgttcgctct ggcgatacac
    45661 ttgtcgtttg gcgcttagat cgtttaggta gaaatatgga agatttgatt tcaatcgtca
    45721 atcgactgaa tgagcgtggt gtgagttttc atagtattca agaaaatatt acgatggaca
    45781 aatcaagttc tacaggacag ctcatgtttc atttgtttgc ggcatttgca gaatttgagc
    45841 gtaatttgat cctggaacga tccgcagcag gacgagcagc tgctcgtgca agaggacggt
    45901 ttggtggtcg tcctgaaaaa ttgtctgaac aagatttgga attactgaag actttagtgg
    45961 atagcggtac acctattaag actattgcgg aacggtggaa cgtctcaaga accaccattt
    46021 atcgttattt ggcaaaaatg gaggagaaaa atggaaagac aaattctcat tagtttttgt
    46081 catttaatgc tgaaaatttt tgccatttat ctagaaaagt gacaatagta tgagaatttt
    46141 ttgtaagtgt ctactgataa cttattctca aatgttgtaa ggattttatt catttgttaa
    46201 tattaa
//
LOCUS       DAZKOW010000009        46094 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000009 DAZKOW010000000
VERSION     DAZKOW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.009"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            207..1208
                     /locus_tag="ACG912_000535"
     CDS             207..1208
                     /locus_tag="ACG912_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFQ0511881.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQSMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            1227..2246
                     /locus_tag="ACG912_000536"
     CDS             1227..2246
                     /locus_tag="ACG912_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFQ0511882.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(2348..3064)
                     /locus_tag="ACG912_000537"
     CDS             complement(2348..3064)
                     /locus_tag="ACG912_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0511883.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3033..3818)
                     /locus_tag="ACG912_000538"
     CDS             complement(3033..3818)
                     /locus_tag="ACG912_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0511884.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3809..4558)
                     /locus_tag="ACG912_000539"
     CDS             complement(3809..4558)
                     /locus_tag="ACG912_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFQ0511885.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4856..5380
                     /locus_tag="ACG912_000540"
     CDS             4856..5380
                     /locus_tag="ACG912_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFQ0511886.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5396..6895
                     /locus_tag="ACG912_000541"
     CDS             5396..6895
                     /locus_tag="ACG912_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFQ0511887.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6983..7585)
                     /locus_tag="ACG912_000542"
     CDS             complement(6983..7585)
                     /locus_tag="ACG912_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0511888.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7689..8645
                     /locus_tag="ACG912_000543"
     CDS             7689..8645
                     /locus_tag="ACG912_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFQ0511889.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8950..9906)
                     /locus_tag="ACG912_000544"
     CDS             complement(8950..9906)
                     /locus_tag="ACG912_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0511890.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10080..10640
                     /locus_tag="ACG912_000545"
     CDS             10080..10640
                     /locus_tag="ACG912_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511891.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10633..11457
                     /locus_tag="ACG912_000546"
     CDS             10633..11457
                     /locus_tag="ACG912_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFQ0511892.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11467..12084
                     /locus_tag="ACG912_000547"
     CDS             11467..12084
                     /locus_tag="ACG912_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0511893.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12102..12785
                     /locus_tag="ACG912_000548"
     CDS             12102..12785
                     /locus_tag="ACG912_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0511894.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13468..14751
                     /locus_tag="ACG912_000549"
     CDS             13468..14751
                     /locus_tag="ACG912_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0511895.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14767..15654
                     /locus_tag="ACG912_000550"
     CDS             14767..15654
                     /locus_tag="ACG912_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0511896.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15671..16507
                     /locus_tag="ACG912_000551"
     CDS             15671..16507
                     /locus_tag="ACG912_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0511897.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16510..17133
                     /locus_tag="ACG912_000552"
     CDS             16510..17133
                     /locus_tag="ACG912_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511898.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17130..18422
                     /locus_tag="ACG912_000553"
     CDS             17130..18422
                     /locus_tag="ACG912_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0511899.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18781..18878
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18994..19575
                     /locus_tag="ACG912_000554"
     CDS             18994..19575
                     /locus_tag="ACG912_000554"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFQ0511900.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19578..20885
                     /locus_tag="ACG912_000555"
     CDS             19578..20885
                     /locus_tag="ACG912_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFQ0511901.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21301..21789
                     /gene="purE"
                     /locus_tag="ACG912_000556"
     CDS             21301..21789
                     /gene="purE"
                     /locus_tag="ACG912_000556"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFQ0511902.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21782..22906
                     /gene="purK"
                     /locus_tag="ACG912_000557"
     CDS             21782..22906
                     /gene="purK"
                     /locus_tag="ACG912_000557"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFQ0511903.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22919..24214
                     /gene="purB"
                     /locus_tag="ACG912_000558"
     CDS             22919..24214
                     /gene="purB"
                     /locus_tag="ACG912_000558"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFQ0511904.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24336..25724)
                     /gene="ltrA"
                     /locus_tag="ACG912_000559"
     CDS             complement(24336..25724)
                     /gene="ltrA"
                     /locus_tag="ACG912_000559"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0511905.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26293..26670)
                     /locus_tag="ACG912_000560"
     CDS             complement(26293..26670)
                     /locus_tag="ACG912_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511906.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26926..27654
                     /gene="purC"
                     /locus_tag="ACG912_000561"
     CDS             26926..27654
                     /gene="purC"
                     /locus_tag="ACG912_000561"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFQ0511907.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27654..27908
                     /gene="purS"
                     /locus_tag="ACG912_000562"
     CDS             27654..27908
                     /gene="purS"
                     /locus_tag="ACG912_000562"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFQ0511908.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27910..28581
                     /gene="purQ"
                     /locus_tag="ACG912_000563"
     CDS             27910..28581
                     /gene="purQ"
                     /locus_tag="ACG912_000563"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFQ0511909.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28582..30804
                     /gene="purL"
                     /locus_tag="ACG912_000564"
     CDS             28582..30804
                     /gene="purL"
                     /locus_tag="ACG912_000564"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFQ0511910.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30789..32228
                     /gene="purF"
                     /locus_tag="ACG912_000565"
     CDS             30789..32228
                     /gene="purF"
                     /locus_tag="ACG912_000565"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFQ0511911.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32251..33303
                     /gene="purM"
                     /locus_tag="ACG912_000566"
     CDS             32251..33303
                     /gene="purM"
                     /locus_tag="ACG912_000566"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFQ0511912.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            33300..33878
                     /gene="purN"
                     /locus_tag="ACG912_000567"
     CDS             33300..33878
                     /gene="purN"
                     /locus_tag="ACG912_000567"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFQ0511913.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33875..35410
                     /gene="purH"
                     /locus_tag="ACG912_000568"
     CDS             33875..35410
                     /gene="purH"
                     /locus_tag="ACG912_000568"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFQ0511914.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35426..36700
                     /gene="purD"
                     /locus_tag="ACG912_000569"
     CDS             35426..36700
                     /gene="purD"
                     /locus_tag="ACG912_000569"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFQ0511915.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36822..37736
                     /locus_tag="ACG912_000570"
     CDS             36822..37736
                     /locus_tag="ACG912_000570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFQ0511916.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37869..39434)
                     /locus_tag="ACG912_000571"
     CDS             complement(37869..39434)
                     /locus_tag="ACG912_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0511917.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39427..40878)
                     /locus_tag="ACG912_000572"
     CDS             complement(39427..40878)
                     /locus_tag="ACG912_000572"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0511918.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40892..41908)
                     /locus_tag="ACG912_000573"
     CDS             complement(40892..41908)
                     /locus_tag="ACG912_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0511919.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            42128..43216
                     /gene="chvE"
                     /locus_tag="ACG912_000574"
     CDS             42128..43216
                     /gene="chvE"
                     /locus_tag="ACG912_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0511920.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43270..44796
                     /gene="mmsA"
                     /locus_tag="ACG912_000575"
     CDS             43270..44796
                     /gene="mmsA"
                     /locus_tag="ACG912_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFQ0511921.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44798..46003
                     /gene="mmsB"
                     /locus_tag="ACG912_000576"
     CDS             44798..46003
                     /gene="mmsB"
                     /locus_tag="ACG912_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFQ0511922.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
BASE COUNT        14419 a         8073 c        10121 g        13481 t
ORIGIN      
        1 aatcacttta cataagtttc aaaagtttct ttaatttcct attgttcaat aagtaacaat
       61 gtaaagataa aattaaaaat gttaatacgt attagcaaaa taaaagcttt ttttattgtg
      121 aataaatgaa cataattagg aggagaaaga tgaacttatt tgacattttt cgatcgactt
      181 tgacagatat gtcgattgtc agtgcaatca cttcaacagt ttttattata ttattagggt
      241 ttgtttgtag aaaaaagggg attttttctg aatctgttgg aaaaattcta tcgaaagtcg
      301 tcttaacagt ggctttgcca gcactggcat ttcgagcttt catgcaagat atcaatacag
      361 aattactgat gcaaggaatc aatgtcttga tttggggatt agttatttat attattctta
      421 ttctagtttc aaaacctttt ttcttaagct ataaagggga taagcaaaca acgttgcgtg
      481 tcctgacgat ctttggttct acgacctttt tcggaacgcc tatcgtcagt gcgatttatg
      541 gtcctgtcgg cgtgatgtat tcctcaattt tcaatattgg ctaccgaatc tttttgtatt
      601 cttatggata cataaaaatg agtggcttga agatggaagc taaaaatatc aaagcgatgt
      661 tcgtcaatcc aatcattatc gctacttttg ccggcctttt catttggctg ttccaagggt
      721 atctgccaca agtgtcagtt acaggtgcag aaggagagat aagccaagta gcgtttttgc
      781 gaattgacca gactgcaccg tggctatttc agtcgatgga ttatttagct ggtttaagtt
      841 caccattggc gtggctagca atcggcgcaa cattaggtga agtaagcttc aaaaaagctg
      901 catctgaaaa aacttcatgg tattacagtc tagtcaaagt ggtatttgta cctgcattga
      961 atatcgtgat tctagcagtt ttgacggcaa cgaatattct accagtcaat tttgaagcac
     1021 tggcaacaat cgttatcatg atggctacac cagctgcaac agtggcagca gcctatgcta
     1081 tcagttttga tagggaggca cttttagcct ccaatgcatc cttactgtca actatttttg
     1141 ctgtggtact gacacctgta tggatcgttg tattacaaat gattcatggc ataggtttat
     1201 tttcataaga aatggaggaa gaaagtatga gaatcgcatg ttacggtgtt cgtccgaacg
     1261 agatagattt ttttgaaaaa ttgaatattt accattatga tctcagtctt tatgaagaac
     1321 tgttgacaca tgacaatatc gtgtcagcaa aggaccatga tgctgtattg ttgaggggaa
     1381 attgtatagc agatgagacc aatcttgcaa aaatgcagga atatgggatt cgttatgttt
     1441 ttacacggac tgtgggagtc aatcatatcg atctacaagc ggcagctgat tttggtatga
     1501 cagtagcccg tgtaccatcg tattcgccaa atgcgatagc agagttatct ttgacttttg
     1561 ccatgatgct tttgagaaat actgcgtata cgacgatccg aacatctttc aaagattttc
     1621 gagtggatga gcaaatgttt agtcgtgaaa tcagaaattg cacagtaggg atcataggaa
     1681 caggcaggat agggttgacg gaagctgttt tgtttaaggg actaggagcc aaagtgatcg
     1741 gctacgatat tcatcagaca gaagctgcta aaaaagtatt gacgttccac tcgcttgaag
     1801 agttattagc caaaagcgat attgtcagta ttcatgtacc tcatatccca ggtgaaaacg
     1861 atcaaatgat caatgaagga tttttagctc aaatgaaaaa aggtagcatc ttgattaata
     1921 cagcccgtgg agagcttcaa gataatgaag ctattttaag agctttgaaa agcaatcatt
     1981 tagctggttt tgcaaccgat gtttttgcaa atgaaacaga ggtttttttc cgatccttca
     2041 agccatggga gacgattcca gaccctgcta tccagcaatt agtagaaatg taccctagag
     2101 ttcttgtaac cccgcatgta ggttcaaata cagatgaggc attatcaaat atgatttcga
     2161 caagtttcga gaactttcgt gaaataatag aaactggaaa aacgaaaaat gaggtatctc
     2221 ttcctaaagc ccgtcaatta agataagaaa acatggatat tcgtcaataa aatgtttaaa
     2281 aatagtccaa gtaaacagtt gttacttctg tatacttgga ctattttgta tgggataatt
     2341 cgatgactca acagtaatct gtgagctttt tccgcccatt ctttgtcaat tcataacagg
     2401 ttagcggccg gccgatttcc aaatatttga ttctttcgtt caatatatct tgttctacta
     2461 aaaaattcaa atatttcttg acggatatac gtgacagtga gcttttttga gccagttctt
     2521 ttgttgaaaa aatagtatct tcttttagaa taaatgcact tatttttttt aaagtcagtt
     2581 ttgacagacc tttaggcaaa tcgatgaatg atggtatttc gtgaatggga gtggtgtggt
     2641 tgaatacttg atcgatcaat ttctgatcgg ctttttcagt cgtttggatt gtctgagaga
     2701 aagcaagata cttttcgatc gccagctgaa aacgttgcaa agtgaacggt ttgatcaaat
     2761 aatctaaggc accgtagcgg atcacattct ttatggtttc gatatcatct gcagcagtga
     2821 tcaaaatcac tgggatatca tttccttgct gtctaagcca agataaaaaa tggataccac
     2881 tttcgccagg catgtataca tcaagcagca gcagatctgc gtttttttgt ctgaaatatt
     2941 tttttccttc ttctacattt ctgaaacttt cgattacgtt catattaggg atgctttcca
     3001 caaaagtacg attcaattct gcaaccattg gatcatcttc gattatcagg acatttgtca
     3061 tcattttgat cttccttttt tggtaattct agatagacga ttgtaccgcc atttgtttgt
     3121 ttggaaaatt ctaaaaatcc accgtggcgt ttgacgattg aatggatgag attcaatcca
     3181 aacccacgat tgattccctt cgtcgaaaaa cctagcgtaa atattttttc tgagatttct
     3241 tctgatatcc cgacaccgtc atcgcagacg gtcacgatga atacttcagc ttctgtgtca
     3301 tagtttaaat gaatccggac ctgtccgttt ctttcatgtt tttgtagcac ggcttctttt
     3361 gcattatcaa gtaagttccc taacacttgc aaaagtgaat ggatggagtc atccatctcc
     3421 attttaggga taaaagaatc gctttctaac agcatttcaa cagattgttc tttggcttcg
     3481 tttattttgc ctaataaaaa accggcaatt gcaggtactt tgatcagact tgagacttgg
     3541 ccaatctctt ttttataatt ttgatttagc tgctggataa aatgggtgac ttcttcatac
     3601 ttcttcaaat caatcaaacc agaaatgaca tgaagtttat tcatgaattc atgggtttga
     3661 gctctcatgg agtttatata ttgtttcgtc cccccaagct cctccattaa tttcattact
     3721 tctgatcgat ttcgtaaagt tgcgactgct cctaaaaaac ggtcattgct aaaaagaggt
     3781 gtaacgctag aaatagtttc tatcccattt aaatacatgg attgagtccg aacggtcgtt
     3841 tgatgctcaa tcgtctcttt aaacaaaacg tcatatagat tatcactgat ttttttccta
     3901 cttcgaaatc caatagctgc tctgtttggt agaaaatgtt ttttgcaggt gtattgatca
     3961 agaggatagt tttatcagaa gagatggcta agattccttc atctacttca ttttcgataa
     4021 tcgttttttc acttaactgt tctgctattt cttgtggttc aagtccaagc agtacttttt
     4081 tcattttttg ggcaagaaaa acggcgccta tgatgcctac gctaagtcca ataatcaatg
     4141 aaaaaacaat cattttttgt ccatctttga cttgattgtc aatcgtgttg tttgttaagc
     4201 caacacaaac tgcaccaata atctcttgat cataataaat agggacgaaa tagcggaagc
     4261 cgaaccctaa cattccttct ttttcagaga aaaattcttg tcctccaatc acttttttca
     4321 catcttcatg acttgagaaa ggaagaccga tgtaacttgg gtctgcgtga gaatatcgaa
     4381 tgccttcagg gtccatgaca acgataaaat ctaaattcga tgcaatcatg atttcatttg
     4441 ttattccttg aatcgcatgt gtcgggtcac cagtctgcag ctttttttgg atgagtttat
     4501 cattggcaac gattttagca atgctagaga ttttatctga cacactagat cggttcatcg
     4561 aaagaataaa attcctgatc aatatggcag aaacaatcaa agaaacgaat gtggagaaac
     4621 aaacgacaag gatgatcgtg ttctggagct ttaatggttt tttcttaaaa aaaggaaaca
     4681 taggctacct cgaaagaatt tattgtgaaa attttaacat gaaaacaaaa atttaacaaa
     4741 ttgtcaacga taaacacaac cttcctataa aaaacaccac tgagtcggta aaagaagcta
     4801 tacagaattt tcaaacattg gtatgataga cgtagaaatg gaaggaggcg tgtatatgat
     4861 agggggaatc gcacttgaga cgatttattg gtatacattg ctgatttgtg caggattggc
     4921 agtcctattg attatttttg gagatgtatt cgattttgat ggcccgcttg atccgatgct
     4981 tattatccct tggttaactt tcacaagctt atttggtttt ctaggtgagc gtttaacaga
     5041 tcaatcatct ttatttttat ttattgctag tggaggcata tcgatagtgt tggtattttt
     5101 gcttaatttc tatatattga tgccgatgaa acatgccgaa tcaacgatgt cgtcttctga
     5161 aaaaactctg gaaggacaag tagctgttgt gatcacaccg atccctgtac gaggtatggg
     5221 agaaattcag ttgaaaagtg ttaccggttc cattagtcgt ccagcttgtt tttacagccc
     5281 ccaagaacag acagctgagc ggggaacgaa agtattgatc attgagatca aggaaagaat
     5341 ctgttatgtc atgccttatg aaagcatgat aaaagcttaa aggagcgtgt gttaaatgga
     5401 aataccagtt gtttttatcg cgatcgcaat cgcagctttg attttactga tgttgctgat
     5461 cgtctttgta gcgaagtacc agacagcaaa accggatgaa gcgttgatca tcagtggaag
     5521 ttatttagga agcaaaaatg tgcacattga tgaaagcaat aacaaaatca aaattgtgcg
     5581 tggaggtggc gcattcgtct tgccagtttt tcaacgttcg aatcggatca gtctattatc
     5641 gagtaaattg gacgtctcca cccctgaagt gtatacggaa caaggcgtac cggtgatgtg
     5701 tgatggtact tcgatcatca aaatcggttc atctgtagaa gagattgcta cagcagcaga
     5761 acagttttta ggcaagacaa gagaagaact ggaaaatgaa gcaagagaag tattagaagg
     5821 acacttgcgc tcgattcttg gttctatgac tgtagaagaa atctatcaaa atcgcgataa
     5881 gttcagtcaa agcgtacaag aagtcgcttc tgtagacctt gcaaaaatgg ggttgatcat
     5941 cgtttctttc actatcaaag aggtcagaga caaaaacggt tatcttgact ctttagggaa
     6001 accaagaatc gcccaagtca aaagagatgc agatatagca gaagcggaag ctctaaaaga
     6061 aacgcgtatc aaaaaagctc aagcagaaaa agaatcacaa gcagctgaat tacagcgaca
     6121 aacagagatc gcagaatcat taaaagaaaa agaactgaaa ttagcaacct acaaacaaga
     6181 acaagatgtt gctaaagcaa aagcagacca agcctacaat ttagaaagtg cccgagcaca
     6241 acaacaagta atcgaacagg aaatgcagat taaaatcgta gaacgtcaaa aacaaatcga
     6301 actggaagaa aaagaaatca cacgtcgtga aaagcaatat gattcggaag tgaagaaaaa
     6361 agcagatgcc gatcggtacg ctaaagaaca agaagcacaa gctcaaaaag tcaaagaagt
     6421 gactgaagcc gaagcagaac gattccgtgt ggaagcactg gcagaagcgg aagccaataa
     6481 gacacgttta gctggacaag cagaagccga agcagcacta gcaaaaggaa aagcagaagc
     6541 cgaagcaaaa caaaagatcg ccaatgcctt caaggaatat ggcgaagcag ctgtcttgag
     6601 tatggttatc gatatgctac cgcaattgat gcgcgaagca gcacagccgc taggaaatat
     6661 cgaaaaaatc tctgttgtcg atacaggaag cagttcagga gaaaccagtg gagcgaaccg
     6721 agtgacaaat tatgccacaa atttgctttc ttctactcaa gaaacactaa aagcgacaac
     6781 aggacttgac ttgaaggaaa tcgtagagaa tttgtcgaca cgtgggacta ctagccaagt
     6841 aaatatgtat gaagcgccaa aagaggcaga acaaaactcg atgaaggaag aataaagtag
     6901 tacacttcac cgttcaatca aaaatggagg aagacttttt aaagttcttc tctccatttt
     6961 tttattttat tgcaaaagaa aattagtcct cgctattttt cttttgtttt tggatcttag
     7021 cttctatctc ttgtaaattt gttcttagca atcgttcttt cttttggagt tcgaccaaat
     7081 cttctgatag ttcttccttc ttttctgttt caggaggacg aagaaagaaa ttgataccga
     7141 ttgcaatgaa tgtccctata aaagtatcta gtacacgttg gatagcatac aaagcggatt
     7201 cgccttgagg gatgctcaac gcaatcagca acaacgtagc tattgcggaa atgataccgg
     7261 aattattgtt aataccatct gacacgacga tcacaataat aactagaata ggaagcaaga
     7321 tcaattctac caaaaaatca ttgtgaaaat agcttttgac aaaaaaataa atgatagcta
     7381 atgtaccacc taatgtattg ccgatgatcc tagactttcc aaaagaaacg gttgtagtca
     7441 agtcctgacg caaagaaaaa acagcagata atgcagcaat catcgggatt cctcgttgag
     7501 tcaaatgaaa aaacaagata cataaaaaaa cagctaaggc acttttgatc gtacgcatac
     7561 ctagtcgaaa acggccaaat tgcatagaga ttctcctttt tggtcttttt actatcatac
     7621 ctaaactttt gtctgatagc tagtttttca ttattcaagt ataattaaaa taaaaaaggg
     7681 ggaagtaaat gcgtatagga atgcgaacag tcaaaacagt gatcagtgtc ttcttttcga
     7741 tggtcacagc gggtagctta tcgctgttgt attggccagc tgcagggatc attgcattat
     7801 taagtgtagg aaataccaag cgctctacat tgcgtacggg catgaatcga atcgcagcct
     7861 ttatattagc tacggcaaca gcactgttca gtttttttgc tgttgggtat actatttttg
     7921 gcttcagtct ttttttattc ctgttcatcc cgcttgcctc tcgcttcaaa ttaaccgaag
     7981 ggatagtagt aaattcggtt ttggtcactc attatttagt ggaggaaaac atgtcttggc
     8041 agctgatcgg caatgaagcg ggattaatgg cgattggatt agtatttgct ttgcttgcaa
     8101 atgtttacat gccagatcgt acgaagcagc tgaaggaaaa ccaaatacag atcgaaaaag
     8161 aatttcgaaa tattttgaga caaatggcag aatttctttt atcagaaaat aaagaagatg
     8221 tgcagatcaa atgcgaacat ttactgacat tcattcgcga aagccaagaa gatgccagag
     8281 aacatcaaga aaactactgg ctcagacagc cgctatacta cgaaacttac ttctctatga
     8341 gaagagcaca ggccaatgtg attaaagaca tgctggaaaa tctagagaga atccagcaac
     8401 cagcttatta tggaaaacat atctatggtt tattgattta tactgcagaa acattttcag
     8461 agtcaaatga tggcagacaa attttaacac gtattgaaga agtttatgta ttgtatcggc
     8521 aaatgcctct gccgactagt cgccctgaat tcgaggatcg agcagaattg ttccagtttt
     8581 tgcagtcttt taaaagtttt attgaaatca aggcagaatt ctcccaacaa aaaatccaaa
     8641 aatagaggtt gtgaattcag ctaatattca ggtcaaaaag ctgatttcac aacctcttct
     8701 ttaaacggtg ttctcctgtc aactcctcgg tcacaagtca caatattcaa caaacatgag
     8761 aagctgtttt ttgaaattgc tccttgtgac tcggagctga acggtaggct cacaacctct
     8821 ttcttaaacg gtgttcaaaa gctttcctct gcggtaataa gtccagccaa acgaaaaata
     8881 ggaggagcta tttttgtttg gctgttctta ttacttgatg cagaacagct ttttcacaac
     8941 cttttaaaat tacatatttt tccatagaac ttgcatttga tccaagattt cgatgacatc
     9001 ttttgtcaag aagagagagc gattgggcaa gtcaccttca atcatgtgta ccatattctt
     9061 tatttcgtaa ttcattgctt cagatgtatt cccgctttcg atgaattcct ttgtaccatc
     9121 tgtaaataag atctccgcac gatcagcacg cgggtaatca gcaatcgtca cataaccgtt
     9181 ctcaaaggca acgataccga cttttggcat ttttgcctga aaagtcaagc tgacagcagc
     9241 aagttcattt tctttgttac gcaagatagt gacggattgt tcgtccacgc ctgtttcaaa
     9301 aggaaccatt gtagaggcaa ctacttcagg ttgagagctc aaaaagaaac gagcgaaaga
     9361 tacagcatac gtaccgatat ccaacagagc tcctccagct aattctggat tgaagaaccg
     9421 gtttttaggg tctggttctt tgtaactgcc gaaaggtgct tggatcattt ttaaagcccc
     9481 cagttttcct gtatccatta ttgagcgtaa ctgctgataa agtggcatgt tgaagattgt
     9541 catcgcttct gctaagatca aattcttttc ttctgctaag cgcatagcgt cagccagttc
     9601 ttttttattc atcgtgatgg ctttttcaca tagaacgtgt ttgcctgctt ccaatgctgc
     9661 caagatatgg tcgatatgct gtctatttgg tacagcaatg tacacgatgt cgatttcagc
     9721 atcgtctaac atttcttgat agcttccgta agcttttgga atattgtaac ggtgagcgaa
     9781 gttttccgct ttttctgaag ttctggaagc aactgctgtt agttcgctcg tctcttgatt
     9841 gaagctttcg gcaaactcat gtgcgatggt accaagacca acgattcccc attgatattt
     9901 tttcatctga aaacgctcct tcatagttgg attgagtttc ttgtctgtct attattataa
     9961 caaaaatcac gaagaataaa gaggagagga cttgaaacat taaaaaaaat gaaagcagcg
    10021 atttaagagg tgctttttga tcaaaactta agtagaatag ttaaaaggag gggataaacg
    10081 tgcatcaagt atatattgat cttattatga gtgtattatt agaacagttt gaaactgaaa
    10141 aaaatttttt agatgaatac ttgctgatca gtccagaaca atggcaaaaa tggaaagatg
    10201 gcgagaaaaa tctgccatct gaagcgatgc agaaagtaaa aaatcttttc actgattatg
    10261 aatggatgct aacgcaaaag attttgcgtc aaacgattat ttttcccgaa aaacgaaacg
    10321 ttgccgttgc agagtacaag caaatcaaaa cacgcatcgc gcaaaaatgg cttagcagca
    10381 aagcagcaaa agtcgagctg atccctttga aaaataatca gcaggctggt agataccttg
    10441 acttgaaagt aagtattcaa tatgatgaat ggggctatga tgatattttg aattttcgtc
    10501 tgccagcaga tgttcaaaaa cagatcgaag gagaaaaaat cgaacttctc gattgggtga
    10561 atgaaaatct ggaagaaact tacgttcatg aggcgaatgg ggaacgtgaa gaacaacgaa
    10621 aggatttttc aagtgaataa atttttagga tttggagcag ttgtgattat gatcgcagca
    10681 atgggctatg ttatttttag cgatgatgat tcgcatgttc aagccggcaa tcaagcagtc
    10741 gaacgaacaa gcaccaaaac aactgctagt aaagaaaaga acagtcaaac aaccagcagc
    10801 aaggtaaagg acaaagaaac aaaagatcgt acgtccaaac aattgccaga tgttaaaaca
    10861 gacgattgga agctagtttt ggttggtcca gatcacaaat tagaaaaaga aattgatgag
    10921 tctactcaat tggcatcatt aagtaacgga tacttgatcg acaaacgcat tgctccagaa
    10981 tatgaagcat ttgccaaggc tgctgaagca gctggatttc cattagtgat ggtctcagct
    11041 taccggtctg tatcatccca acaacaagtt ttcgaacaaa atgtccaaga tgtgatgagt
    11101 cgtcaaggtg tatcagaaga ggaggcgaca aagattacta aagaaacgat tacggagcca
    11161 ggatacagcg aacaccacac aggtctggca gtcgatgtcg tcgatcaaaa ctggtacaac
    11221 agttatcctt ctactgtatt ggatgcaagt tacggagatc agcctggagc taaatggatc
    11281 gcagataatg ctgcgcagta tggatttatc gttcgttatc caaaaggaca cgaagatata
    11341 actaagatca catatgaacc ttggcactta cgttatgtcg gtaaagaaaa cgctgagtat
    11401 atcaccaaac accaattgac gctagaagaa tatctgaatg aattaaatga aaagtaggaa
    11461 ttgatcatgg cgaaaaaaaa gtggaaaaag caaaagcgca agaatatccg ttggccagca
    11521 gtggttgccg gtgtagcagt gatactctta gcctttgttt tttccttgaa gaatctgtct
    11581 tctcctttta ctgataatca gcaagaaaat gaagcgcaat cccatcagca atttattgaa
    11641 cggcttgttc cgcatgccca agaactacaa gatggttatg gagtgcttcc tagtatcatt
    11701 ttaggacaag ctatattaga atcaaattgg ggaaaaagta ccttggctag caaatacaat
    11761 aatcttttcg ggatcaaggc ttatggggat cagaagaaag taagcctgga aacaaaggaa
    11821 tttgttaacg aagaatggat aactatccaa ggagacttca aagtgtatga ttcatgggaa
    11881 cagtccatgg atgaccatac acaactgttc gtccaaggag tagattggaa tcctgcgctg
    11941 tatgaaaaag tgattacggc caccaactat caggaagccg cacaagcatt gcaagatgca
    12001 ggttatgcga cagaccccgg ttatgcgcaa aaaatcatcc aggtcatcga gacgtatcaa
    12061 ttgaatcagt atgaccggag atagcaaagg agaagtggct gatggcagaa caatttacgc
    12121 cagaaatcat gacggaattg agaaaagata tcgtaagagt accggaaatc attttagaag
    12181 caagcgggat caagatattt ggaaaattga tccgttcgat catctttact acggatattg
    12241 cgatcatacg taatacaaat gcgaatgcag taattgctgt gtatcctttt acacctcatc
    12301 cagcgattac taaaagcatt attgaggcag ctgacattcc agttttctcc ggtgtaggtg
    12361 gtggtttgac acaaggtgct cgttcttcct acatgagcat gttcgcagaa gcgcaaggtt
    12421 cgttaggggt cgttttgaac ggtcctacgc ctttagagac agtaaaagaa gtttgtcgag
    12481 tagttgatat tccagttatc agtacggtaa cgtcgaaata tacagaaata gatgaaaagt
    12541 tagcagctgg tatttcgatt atcaatatca gtgcaggtaa agaaactgca gaagcagtac
    12601 gccattttcg taaaaaatat cctgaacttc ctatcattgc gacaggtggt ccaacggaag
    12661 aaagtattgc tgagaccatt caagcaggag caaatgccat cacatatacg ccaccaagta
    12721 gcggagagct ttttagtcgt aaaatggaaa aatatcggga aactgagaaa aagaatcatg
    12781 aataacaggt gagaatcaaa agacgaggaa gagcagatag tggctcttcc tcgtcttttg
    12841 tactccagta ctatttgttc agaattcaaa aaaaagagaa atttttttgt ttttttacct
    12901 gtaatgtaca taagaagagt taaaaccttt tgttattatt agcattctaa caaaaaaatg
    12961 tcttgattta ttgaagcgct ttctatagaa tgaaaaacga ataaaaacta aaaaaggcac
    13021 ccgacaattt agtagcagct aaattgtcgg ggcttgaaaa gtcagcggga actgaccaat
    13081 caagttgcca tagtcaatgc ttgtgcatcg acgtgtatat tgtacctaat ttcatacaga
    13141 attactagtt atttttctgt tttttaggga agagcgcaca cgaagaagca tgcttattta
    13201 gctatgtcaa ggatattggg cagttgtctg atatcctttt ttctttgcgt aattatcctc
    13261 ccatcattga agcaagaagt gtttttagcg atgaaagaag aatcagcact atctaatttt
    13321 tcttgaaagt attgagttgt tcatcctagg cagaacgata aggcaagtct tggtttttat
    13381 tccccttgaa aaagatcgtt taataaatat gttctgtata aaaatacagt atattgaaaa
    13441 ataacaatgg aagtagaggt gcagagaatg aagaaaagaa tgagaatcat cttagcttta
    13501 agtgttatgt tattcttctt gacggcttgt gggcaagaag atgacaaaaa aatcacgctg
    13561 aacatggctt ggtggggttc gcaagtccga cacgatgcaa cagtaaaagt aatcgagctt
    13621 tatgaagaaa aaaatcccca tgtgacgatc gattatgaat tttatgatta tgaaggatat
    13681 tacacaaaat taggtacact agcagcatca gatgatatct gggacatttt ccaactgaac
    13741 aatcaatttc ctcaatacat caatcagatt gaaccattag atgaatatgt ggagcaaggg
    13801 ataattgata tttctaatgc agatgaatcc tatttagcaa cgacgactta cgacgggcaa
    13861 ttagttggac tgtctaacgg ggtgaattcg tttgcgattg cttacaataa aaatatattt
    13921 gaacaattaa atatccctga accagcttca aattggacat gggatgaatt tgaacaaatc
    13981 agctatcaaa tcactgaaga attaggaatc tatgctattt caagattcga agattttata
    14041 gctggctgta ttattcaaat accacaagtt gagaaaggat tgaattttta taatcgggaa
    14101 gatctatcta acagcttggg atttgaaaat ccagactatc taacagattt tttccagctg
    14161 cgtaaaaatc tggtagacaa aggggcccat ccagaaccag gtggtggagc tgcaagtacg
    14221 acggatctgt cttttcaggc ggttagggat agagaagcgg caatggtgtt tctatcaagt
    14281 aaccagttga cagaaatatt gtcaggggct cctgaagata tggagattca gttgattaat
    14341 ccacctcgca gaaaagcaga tggagaatct ggtatccctt tacgttcttc gcaaatgctg
    14401 agtatggcaa aaagctcagc ccataaagaa gaagctgcta agtttattga tttctttcaa
    14461 aacagtgaag aagccaatga aattttaaaa ggagaacgtg gtgtaccaat catgtcgaat
    14521 attcgagaac agctcgctcg tgcagatgat gcaactttgc aagcgacatt tgattattta
    14581 gaaatgattt ccgatatgga caacggcgaa gtgaatgtct ttgatagtgc tttgaatccg
    14641 gaaattcaag accaatacaa tcttttagtt gaaaaagtca tttatgaaga agtaacagca
    14701 gaagaagcag cagagagtct ttatgagttt gcgtcaaaca ttctaggata gcagcaggag
    14761 gaaggcatga aaaaagaaaa actaaaacgg ttcttggatc aagacaatgt ggtcggttac
    14821 gtatttgctt ctccattcat cctaggattt ctattgataa cagtgatacc gatgatcttg
    14881 tcactttatt attcgttcac cgattatcgt ttgggaaatc cagtctcatg gatcggcttg
    14941 gataattata taaagatgct gagtgatgcc cgttttttga actccatccg agtcactttt
    15001 ctctatgtat ttgcatctgt acctgtgaaa ctgatctttg gtctattgat tgcttatctt
    15061 ttaacgagga atatcaaagg aacgactttc tttcgttctt tgtattatgt gcctagtttg
    15121 atcggtggca gtatttctat cgctctagtt tggcgggagt tgttttctaa aaacgggtta
    15181 gtgaatattt tcctggaacg attaggcgca cctgcgcttt cctggtttag cgatcaaaaa
    15241 ctggtcatga tccctcttgt tttgatgtct gcttggcaat tcggttcggc aatgatcatc
    15301 tttgctgcag gtatcaaaga gatcccaggc tcgtattatg aagcggcaga gatcgatgga
    15361 gcgaacaaat ggcagacgtt ttacaagatc acgttacctc ttctttcgcc gatcattttg
    15421 tataacttgg ttatgcagac aatcgcggcg tttatgactt ttacgcaggc atttatcatt
    15481 acccaaggtg gtccgaatga tgccacgaac ttttatgctc tttatgtgta taaccaagct
    15541 ttttctttta attctatggg gtatgctagt gctatttcgt ggatcatgct gttgattatg
    15601 ggagctgtca cctttctgat tttcaaatca tcaaaaaaat gggtcgttgt gtgaatgaga
    15661 ggaggaagaa atgaataata cagtaacgaa atggtttcaa tacggtttta ttttgctatt
    15721 aggtctagtc atgatttatc cggtcttatg gatgatcgca ggttctttta aaacggatca
    15781 agagattatg agtggttcgc tgaatttgat tccagagact tttcaatggg aaaattacgc
    15841 aaaaggatgg gcggggtttg ctggaatttc attttttact ttctttagga attcgtttgt
    15901 catctctgca gtttcgacga tagggacagt tctgagctcg acttgtattg cttacgcatt
    15961 atcgagaatc gatttcaaag gaaaaagatt ctggttcgtc gtcatgttag taacgatgat
    16021 gattcctgca caagtgattt tgattccgca gtttattatt tacaatcggc tgaatttggt
    16081 tgggacgtat gtgccgctga ttttaccaca tttttttgga caagcatttt tcatttacca
    16141 gatcatgcaa ttcatggtca gcatccccaa agagttagat gaatcagcga ttattgacgg
    16201 atgcagcaag tatagtgtct ttaccaaaat tatttttcct ttgctgaaac cttcgatcat
    16261 cacgacgatc atcatccagt tttattggaa atgggacgac ttcatgggac cattgattta
    16321 tttaaacaag ccgagatctt acacggtatc gattgccatc aaattatttg cagacgctgg
    16381 atctagtacg acttacgcga gtatgtttgc gatgtctacg ttgtcgctgc taccggtttt
    16441 tctgattttc ttattcttca ataaatatct tgtccagggg atcagtacaa gcggtctgaa
    16501 gggctgaata tgaaaaagcc aatagaagag atgtttcttt acaagctcaa ctactatgtc
    16561 tgtcggattt ttttaaccag tcttatcagt aatgctcttt tcgctttagt tctaggatgt
    16621 ttgttcactt taggattagt gctgtctgct cgtgttgtct atctgattta ttttttgatg
    16681 atgtatcctt cttattatct atctgtccat tacttggcag aaaaaaaggt atgttggagg
    16741 aaaggaaaaa tcaaacggat gtgtttcatc attacgttgt ttcagctgtt tgtcattatt
    16801 tttgcagttg attggtattt attagtggga aagattggag cttggtatct gattcctttt
    16861 ctattgtgtc tgttgctttt tgtatcccat agttttttta gtacgctaat tgatatctgc
    16921 aaagaagata aagcagatag tcttgtttat ttgatcaaaa ggaatatctg gctgttgtca
    16981 aaaaacgtca aggggtttct gtggtattta tgccttttgt atggactgtt ctggtgtata
    17041 cagcatgatt ttttactcac tgcattactg tatccagggc tgccatttgt cacagtattt
    17101 tgcttaaaag aaaaggagaa tgactttaaa tgaagaaaaa atatgtacaa gtcggacttg
    17161 gtgggagagc gcgttttttt tatgaggcaa ttgcctctac gtatcatcaa acttgtgaac
    17221 tagtcggttt ttgtgatgta tctcaaacaa ggatggatta tgcaaatcgg atacttgtga
    17281 acaagtatgg acagaaaaaa gtaaagacat gtatgccatc acaatttgag caactactgg
    17341 atgacgtacg gccggctgaa gtcattatta cgtcagtaga tcgaacgcat catgattata
    17401 tcattcgtgc aatgaaaaaa ggatatgatg ttatttgcga aaaacctctg actatcgatg
    17461 aggaaaaagc tcaagaaata ataacggtcc aaaaacagac gggaagaagc ctgcgagtca
    17521 cttttaatta tcgttatgca ccgtatcaca cgaagataaa ggaactgata gaaagtgatg
    17581 tgattggtga tgtgttttct atccacttcg aatggctgtt aaatacgcaa cacggggcag
    17641 attatttcag aagatggcat cgaaataagc acaatagcgg cgggcttctt gttcataaat
    17701 ctacccatca ttttgatcta gtgaattttt ggttgaacag ccgtccccaa aaggtatttg
    17761 cattcggtgg attaaacttt tatgggatgc ataatgcgga gaaacgcgga gtagaaatat
    17821 tttacagtcg atgctatgga agcgaactag ctaaagctga tccatttgct atagacctgt
    17881 cagccaatga ggaattgcga gaaatgtatt tagaagcaga aaaagacagt ggatatattc
    17941 gtgaccgcag tgtattttcc gatgatatct ctattgaaga tacactaggc gtaacggtca
    18001 aatatgaaac tggtgcgatt ttgacgtatt cgctaaacgc gtatatgccg tgggaaggat
    18061 tcaatgtttc tttcaatggc agcaaaggac ggatcgaaat gaaagtgatc gaaaagtctt
    18121 acatcaatgc aggtggagat aaaaaggatg aaggagcagc cgttcttcag tccatccatg
    18181 tctatccgat gttcggtgag ccatatgagg taatcgtcga acaagaagcg ggcggacatg
    18241 gcggaggaga tccgcgttta ttagatgatc tatttggtga agaaaaagaa gaagatccgt
    18301 ggaatcgggc agcaactcac gtagatggta ttttgtcgat tttgacagga atcgcagcaa
    18361 atcattctat tgcttcagga aaagcagtag tgattgacga gcttgtcagc ttaaaagaat
    18421 aagaatagat ttacggaaat tacttcccgt agtttataaa atcttgcctt attgccgata
    18481 ggctgtcttt tgaacactat ttattcggaa aaaggggcta tgacatgctt tttgtcatgc
    18541 cttcttttta tgtgggctgc ttttttagat aggagaaaat aaaaataact atttaaaccc
    18601 gaacaaaaag gaataatgca ttaaaaatca ttagatttta caaaaaaata agtaaaccac
    18661 gttgtcagtt tcagaaagta gtgttagact gttttcaata tttgaaacaa cattttttat
    18721 tcaaatatgg caaactgcct tcacgtgcaa aacagttgca agaaaatgaa tttacactat
    18781 tattagtttt acatacctac ttatatatcg tcataatatg gatgacagtt tctagccagt
    18841 accgtaaatg ctggactata agtaaaagat tggctatttc acccttcttt tcttatgata
    18901 aagtggaggg tgtttttctt taacagaaac atagtaggtt tttgcacaaa agcggttatt
    18961 tcggtattaa taaatgataa aggagcaaat aaagtgaaag aattagttga acgtattcaa
    19021 aaagacggac gtatattagg cgagggcgta ttaaaagtcg acagcttcgt cactcaccaa
    19081 gtagaccctg tattgatgga acaaatggga aaacggtttg cagaagtatt taaagaacaa
    19141 ggaatcacga aagttgtgac cattgaagct tctgggatcg caccagcttt atttgctgca
    19201 agagaattag atgttccaat gatcttcgct agaaaagcaa aaagtttgac gatggatgaa
    19261 gaattattga cagcttctgt ttattctttt acgaaacaag tgacaagtac gatttctatt
    19321 tctcgtaaat ttttatcttc agaagacaaa gtattgatta tcgacgattt cttggcaaac
    19381 ggacaagcgg caaaaggatt gatcgaatta tgccagcaag ctggagcgca agtagaagga
    19441 atcgggatcg tcatcgaaaa gtctttccaa gatggacgtc agttacttga agaaatggga
    19501 ttgagagtcg tttctcttgc gcgtattgct tccttgacga atggacaagt tgagtttctt
    19561 gaggaggacg cataatcgtg gaaaaagaaa cacaaaatgg aaaagctgcg gttttagggc
    19621 tgcagcatct gctagcgatg tatgcaggag cagtagcggt gcctcttttg atcggtacag
    19681 gactaggctt taatcaagaa cagatgactt atttgatttc aattgatatc tttatgtgtg
    19741 gattagcgac attattgcaa ttgacagtta accgattttt cggaattggc ttgcctgtcg
    19801 ttttaggatg tgcgatccaa gcagtagctc cactgatctt gatcggaacc aatgaaggag
    19861 taggagctat ctacggatct attatcgctt ctgggatctt tgttgtgtta gtttctggtg
    19921 tcttttctaa aattaaaaaa ctattccctc ctatagtaac aggaacggtt atcaccgtta
    19981 ttggtttgac tttgattcct gtagcaatcg agaaaatggg tggaggtaat gcaacagcag
    20041 ctgattttgg tgacaaaaca aatctgctac ttgcatttgt gacgattctc ctaattgtag
    20101 gcgtgcaaat gctggcaaaa gggttcattc gttcaatcgc ggtattgatt ggtttagtcg
    20161 gaggaagcat cttagctgct ttcatgggaa tgattgatct gagcgcgatt ggagatgcgc
    20221 cgttgttcca tgttccacaa cccttttatt ttggtaaacc aactttcgac gtatggtcga
    20281 ttttactaat gatcatcatt tccatggtca gcatggtaga atcaacaggt gtatattttg
    20341 cacttggtga cattactggg aaaaatgttg gagaagaaga attgaaaaag ggatatcgtg
    20401 cagaaggact tgccgttatc ttaggcggta ttttcaatac attcccttat actggctttt
    20461 ctcaaaatgt cggattggtt caactttcgg gaatcaaaac aagaaaaccc atctatttct
    20521 ctgcattctt cttgattgtt ttaggattat tccctaaaat cggtgcgtta gcacaaatca
    20581 tccctgagcc ggtacttggt ggaggaatgt tagtcatgtt cgggatggtg gcagtccaag
    20641 gaatccgtat gctgttggaa gttgatttca gcaatgataa aaatctattg attgttgctg
    20701 tttctgttgg atttgggctg ggtttcaatg ttacgccgac attattcagt caaatgccgc
    20761 aaacattgca aatgttcact ggaaatggca tcgtaatgag tagcattacg gctatcatat
    20821 tgaatttgat ctttaatgga ttgaaaaaag atgaagaaat attggaagaa gccttcaaga
    20881 agtaatagag caaaagaaag gttgtgaaat cagcgttttg acctgattgt tagagaaaca
    20941 aacggaaaaa tagttcctcg tattttgccg tttgtttgag ctaataccga gggtcagctg
    21001 attgaacacc gttgaaggaa gaggtcgtga gcctgccgtt catctccgag tcacaaggaa
    21061 caatttcaaa aaaaagcttc tcatgtttgt tgaatattgt tccttgtgac cgaggagttg
    21121 gcaggagaac accgtttatc cataaaaagg aatgtgacct aacttttgtc acattccttt
    21181 tttttatctt taataaacac gaacaataaa atgtttttct ttgttaacat tcgaaatttc
    21241 tttgacatca aaaaaagtga ttgttaaaat atgaatgtag aaaaactggg aggaaaaaca
    21301 atgacagcaa aaatctctgt gattatgggg agtacttcag attgggaaac aatgaaagaa
    21361 gcatgtcaaa tattggatga attcggtgtg tcttatgaaa aaaaggtcgt ttctgctcat
    21421 cgaaccccag atttgatgtt tgaatatgca caaaacgctc gaaaaaaagg aatcaaaatc
    21481 attattgcag gagcaggggg agccgcgcat ctgccgggta tggtcgcatc aaaaacaact
    21541 ttgccggtta tcggtgtacc ggtccaatca cgaacattaa atggtttgga ttcgcttctt
    21601 tcgattgtcc aaatgcctgg aggagtacct gtagctacta cagcaatcgg aaaagcggga
    21661 gcaattaatg ctggactact tgcgatacag atgctttcca tgtatgattt agaattggag
    21721 gctaaattgg ctgaaagacg ttcaatgttg gctaaaactg ttatagaaag tagtgatcaa
    21781 cttggttaaa cctttgttgc ctggttcaac gatcgggatc gtcggtggcg gtcaattagg
    21841 tcgtatgatg gcgattagtg caaaagaaat gggctttaaa gtaggtgtgc tggatcctgt
    21901 gagcgattgt ccagccgcac aagttgcaga ttggcacatt gaaagtgtct atgatgacac
    21961 ctttgcgctg aaagagcttg ccagaagaac agatgtcatt acctatgaat tcgaaaacgt
    22021 cagtgtagag gcgttaaatg cgattttacc catgtcattt attcctcaag ggacagactt
    22081 gcttgcgatt acgcaagacc gcctgttaga aaaatctttt ttggaaacga ataacatcgt
    22141 gattgctcct tatgcaacga tcgtcagtcc gacggatatc caagatgcga ttgacgggat
    22201 cggctatcct tgtgttttga aaaccactcg aggcggctat gatggcaagg ggcagtatgt
    22261 tttgaaaagt cgggcggatc tagctcctgc catggacctt ttaagagaag gaacctgtgt
    22321 tttagaagca tggatcccgt ttgaaaaaga gatttcgatc atggtggcag gaaatggtca
    22381 aggagatttc acgacttttc cggtcgtgga aaacatccac cacaacaata ttcttcatga
    22441 aacgattgct ccagccgcga ttgatcagga tgtgatcgag gaggctgagc gtattgcccg
    22501 tgtgattgca gaagcagtga gcctttcagg tgttttaggg gtagaaatgt ttttaacaaa
    22561 aacaggcggg ctatatgtga atgaattagc tcctcgtcct cacaattctg gacattattc
    22621 tattgaagct tgttctatga gtcagttcga tgcccatatt cgaggtattt gtggttggcc
    22681 attaggagaa aaagtacgtt tgataagcaa tgcagtaatg gtcaatgttt tgggagaaca
    22741 actgctggaa agctatcgct tgattgagaa taagccagac tggcaattcc atttctatgg
    22801 aaaaaaacaa gcaaaaaaag gtcgcaagat gggacatatc actatcttga cagaagacat
    22861 ccagcaaacg ctggaagaaa tcaaacaaac gaatatttgg gattagagga gaaaaaacat
    22921 gttagaacgt tatacacgcc ctgaaatggg gaatatctgg acggaagaga acaaataccg
    22981 tgcgtggctc gaagtcgaga ttttagcaga tgaagcttgg gcagaacttg gtgaaatccc
    23041 gcaagaagat gtgaaaaaaa ttcgtgagca tgcatcattt gatatcgatc ggatcttgga
    23101 aatcgaaaaa ttaacacgcc atgatgtcgt tgcgtttaca cgagcggttt ctgaaacatt
    23161 gggagaagaa agaaaatggg tccattatgg tctaacaagt acggatgtcg tagatactgc
    23221 ttatggctat ctcatcaagc aagcaaatga tattttacgg gaagacttga aacgttttac
    23281 ggaaatcatt ggcgaaaaag caaaagaaca caaatataca gtgatgatgg gtcggacaca
    23341 cggtgtccat gctgaaccaa ctacttttgg attgaaactt gccacatggt attcagaaat
    23401 gaaacggaac atcgaacgat ttgaacatgc ggcaaaagga gtagaagcag gaaaaatctc
    23461 aggagcagtg ggaacgttcg caaatatccc gccatttatt gaagagtatg tatgtaaaca
    23521 attggggatc cgtccacaag aaatctctac tcaagttctc cctcgggatc tacatgctga
    23581 atatttctca gcgatggcgc ttattgctac aagtattgaa cgttttgcaa cagagatacg
    23641 cggattgcaa aaatccgaaa cacgtgaagt agaagaattt tttgcgaaag gtcaaaaagg
    23701 ttcttctgct atgccgcaca aaaggaatcc gatcggttca gaaaatatgg ctggtctagc
    23761 cagagtggtg cgcggacaca tggtcactgc ctatgaaaat gtcacattat ggcatgaaag
    23821 agatatttct cattcttcag cagaacgaat catcttgcct gataccacga ttttattgaa
    23881 ttacatgtta aatcgttttg gcaatatcgt taaaaatcta acggtctttc ctgaaaacat
    23941 gaagcggaat atgtctgcaa catttggttt gatttatagc cagcgcgtta tgctgaaatt
    24001 gatcgataag ggaatgacaa gagaagaagc ttatgatctt gttcagccaa aaactgcgta
    24061 cgcttgggac aaccaagtgc agttccgccc gctgttagaa gaagatccaa ccatcacatc
    24121 ggtattatct aaagaagaaa tagacgatgc ctttgattat catcatcatt tgaaaaacgt
    24181 tgacgtgata ttcaaacgag taggtttgga ataaagaaga gtgagttgca aaattcaatt
    24241 aaaatagaat agcaaaaata atcgagtaga ggggaaagcc aactgacctt ccgcccctct
    24301 cacaccaccg tacgtacgga cccgtatacg gcggttcaat aagttgagtg tacgaactgg
    24361 tacttcaagg agatgtcctc atatccttgg cgggccagtt ctttgtctgt taatgatcga
    24421 tgaagaatat gacttttgga gactgaccaa ataccttttc ttgtatttga ccattcatac
    24481 gctttcgctt tatcgatccc taaacgaatc agattttttc ttttggttct tggattcttc
    24541 cattgtttcc aaatatattg tctgattctt cgcttcaacc aaccgtttaa ctccctcatg
    24601 aattctttca tctcaccaat tccatagtag ttgatccaac cagtcattag ttgcttgatt
    24661 tcttttaaaa taagttggat acttcttcca cggtttcttt tagtgagttg tttgagcttg
    24721 cttttcacac gctgttttgc ttttgcgtga gggcgaattt taataccttt ctttgttggc
    24781 agaatgcaga atccaagaaa ctttcgtttc aatggacttc ctaccgcact cttttctcga
    24841 tttaccgtca actttagttg ttcttcgaga aattgaatgg atttcttcat cacacgctgt
    24901 cctgctcgtt tgcttctaac atagatattg cagtcatcgg cgtatcgaac aaatttatgt
    24961 cctagatttt ctagcacttg atcaaattcg ttcaggtaga tgttacttaa caggggagag
    25021 agattgcctc cttggggcgt tccttcttca gttgttgtga tgagtccatt ttccattaca
    25081 ccactcatta agtattgacg gatcagtttt aggacttgtt tgtcctttac tttccgctca
    25141 atgaaataca tcagcttatc gtgatttacg gtgtcaaagt aggctttcat atcaatgtcc
    25201 accacatatc ggtaaccttg ttcataataa gattttgctt tcttcactgc gtcatgtgcg
    25261 ctgcgatttg ggcgaaaccc ataactatta tctgagaatt ctggttcaaa tagcggttgt
    25321 aacacttgat tgattgcctg ttgaagcata cgatcaataa ccgtgggaat tcctagtttt
    25381 cttacaccgc cgtttggttt cggtatttct actcgcaaaa caggctgagg tttatagttc
    25441 ccttggcgta aattggatag taattcctcg cggtgctctt tcaagtaagg aagcagttga
    25501 tcgacggtca tcccatctgc accagccgcc cctttatttc tgactacttg aagatacgct
    25561 tgattgagat tattccgatc tagaattctc ccaaacagat tttctacacc atcttgtttc
    25621 ctctgttcac cgcaagaagt gctgcgcact tcatcatacc cttcgtgttc cacactagct
    25681 ctttgatgat agccaagttt tcttgttttc tgcggtcgtc gcatcttctc acacctccta
    25741 aattattcaa gattcttatt gttcagtcct tcgtaacggt cagtgtcgtt actactatga
    25801 cctcggctga cttctcatga ttcaccatca tgtcgccatg atcgttgtgc tggtttgttt
    25861 tcattcaacg cccacaacac cctcatgaga tctcccccgg taagagtgaa aactttcctc
    25921 ccatgtaact gctagattta ctgtataggt ttcgggcagt attggacttt gtcttgtgat
    25981 gaagacttat ccgacctaat tcagccttgt atctagtttc tgttcgtcag ttcaggagtt
    26041 tgcgtccgac ttccttcagc cacaacctca cgattgccgc cttgtctttc gctaacagtt
    26101 cctactgcca agcctgtagt ggactttcac caccaagttt tcacccatgc agggcgcaca
    26161 ccaaaaaaga agcagatccg ttagcggttc tgcttctttt agtttattat attttcttat
    26221 caataatagg aacctgattt taaaagcggc tacctcttta ccgtgcctct ttacatacaa
    26281 aaaatagagg aattaagcgt aaatggcttg gctattataa ctaaaatgct ggttatggag
    26341 atgttgcagc aagttttcga gaaaagcgta ttccatctct tgtccattga ataagatctg
    26401 gatacgataa tgatttttga aacgcttctg ttcatgaatc tgactgattg aaagttcgcc
    26461 tttgtgttct gtctgataaa gaaaagtcct tttacggcat tcagcacagc gcttggttgc
    26521 tcggcggatt tttcggaatg tataaaaact agtatcttta ttgcataaat aaacgtcttt
    26581 gatcgacagc aaatcattat atagacagga acgttcttca gctgttaatt ctgataaaaa
    26641 aagctgacct tgttttattg catcttccat cggcaagtgc ctcctcgaac ccattatcca
    26701 aatttttagt taattctaat ataccatcat taagtataaa tgtaaacgct tgttgatggt
    26761 ataagatttt cgtttctgat aaaacacgaa cattaaaaga atctaatgcc ttaaatgtac
    26821 atatttttgt tgatttataa aaagatgatt gctaaaattt aaagtgaaaa aacgaacaaa
    26881 aaacgatgta tttgctttta atgtaaataa aaaggagaga cgtacatgga gaagaaagaa
    26941 cttttgtatg aaggaaaagc caaaaaaata tatcttactt ctgatgagaa cttgatttgg
    27001 atagaatatt tagatcaagc aactgcgttg aatggcgcga ggaaagacca gataagtgga
    27061 aaaggagaat tgaataatca aatcacaagt gcaattttcc gttatctgac tcaaagagga
    27121 ataaaaaatc attttattga acaattgtct aaaaccgaac aattagcaaa aaaagtacaa
    27181 attatccctc tggaagtggt tatccgaaat attgcagcag gaagtttctc aaaacgtcta
    27241 ggtgttcccg aaggtaaaga attcatcaaa ccaatcattg aattctacta taaggatgac
    27301 cagttggatg atccatttat caatgaagag catatctatt tcttacaact tgcaaagccg
    27361 gaagaaatca gtgagctgaa agcccaagcg ttggctatta atgaaatttt gaccgaatta
    27421 tttgctaagg tcggtttggt tttagtagat ttcaagctag aatttggccg gtcgaatgga
    27481 caaattttgc ttgcggatga agtaacaccg gatacttgcc gattatggga tgaacgtacg
    27541 aatgaacata tggacaaaga cgtttaccgc agagatctag gtgatttgat cccagtctat
    27601 caagaagtat ataaccgtct atcggaacta actgaagaaa aggagcgagt atgatgtatt
    27661 tcgtaaaagt atatgtaacc tataaagatt cagtgttaga tcctcaaggg gaggcagtaa
    27721 aaaaagcagt ccatcgtatg ggatatgaaa caatcgaaga tatccgaata ggaaaatact
    27781 ttgagataca acttgctgat gcagaagaac caatcgatac agtgattgaa acgatttgtg
    27841 acaagctgct tgcaaatgta aatatggaaa cataccgata tgaaatcaaa aaaatggagg
    27901 aagtataaca tgaaatttgc ggtcatcgtt tttccaggtt caaattgtga cgcagatatg
    27961 ttatgggcag ttcgcgagat catgggcgct gatgccgaat ttgtacggca tgatgagtcc
    28021 tctttagaag gatttgatgg ggttttactg ccgggaggat tctcctatgg tgactatttg
    28081 agatgcggtg cgattgctcg gttttctaca atcatggaag aagtgattcg ttttgctgaa
    28141 gaagggaaac cagtattcgg tacatgcaat ggattccaga tattgacaga agcaggactt
    28201 ttgccaggtg tactgagacg aaatgagtct ttgcatttta tttgcaaaac tgtaccattg
    28261 aaagtcaacc ccaagacggc ctttacttca gagtatgaag ccgacgaaat aatctatctt
    28321 ccagttgctc atggggaagg aaattatttc tgtgacgaag aaacacttca agaactaaaa
    28381 aaaaataatc agattgcctt tacttataat gacaacatca acggcagcgt ggaaaacatt
    28441 gcagggatta cgaacaaagc agggaatgtg ttaggaatga tgcctcatcc agaacgtgca
    28501 gtagaagact tgctaggatc agaagatggg aaacggttct ttgcttccat tttaaaaaac
    28561 ttcggaaagg tgaccgtata gatgaaaaca ttgaaagaac cgacagctga agagatcaag
    28621 aaaaaaagaa tctatgccga atggggattg actgatgaag aatatcgaat gatcgaagaa
    28681 gatatcttag gaagaatgcc taattatacg gaaactggac tgttttctgt tatgtggagc
    28741 gaacattgtt cctataaaaa ttctaaaccg gttctacgca aatttcctac tagtggacct
    28801 catgtgttgc aaggccctgg agaaggcgca ggcatcgtcg acatcggtga tggactagca
    28861 gttgtcttca aagcagaaag tcacaatcat ccttcagccg tggagcctta tgaaggagct
    28921 gcgacaggag ttggcggaat catcagagat attttcagca tgggggctcg tccaattgcg
    28981 ttgctagatt cattgaggtt cggcgaatta gataatccaa gaacgaaata tttactagaa
    29041 gaagtagttg caggtatcgg cggatacggc aattgtatcg gcataccgac tgtaggcggc
    29101 gaaattgcat ttgatccttg ttatgaagga aacccattag tcaatgcaat gtgcgtaggt
    29161 ttgattgaac acaaacatat ccagaaaggg caagccgctg gcgtcggtaa ttcgatcatg
    29221 tatgtaggtg caaaaacagg tcgcgacggt atccacggag caacttttgc ctcagaagaa
    29281 tttaatcaag aagaagaaca gcaacgttct gctgtacaag taggcgatcc ttttatggaa
    29341 aagctgctgc ttgaagcgtg tttggaatta atcttgcatc attcggattc tctaataggt
    29401 atacaggata tgggagcagc aggacttgtt tcatctagtg cggaaatggc ttcaaaagct
    29461 ggctcaggac ttatattgac attagatgaa gtcccacaaa gagaaactgg gatgacacct
    29521 tatgaaatga tgttatcgga atcacaagaa cggatgttga tttgtgttaa atcaggagag
    29581 gaagaaaaaa tccaagaact atttcagaag tatgatctag atgcagtcac tatcggtaaa
    29641 gtaacagacg acgggcaata tcggctatat catcaaggag aagaagtagc aaatctgcca
    29701 gtcgatgcat tagctgaaga tgcaccagtt tatcataaag aaatgaaaga accagctcga
    29761 atcgtagaat ttcagcagtt agctccttat cagccagtta tcgaagaacc tggacaagta
    29821 ttgcttgatc tgctgcagca accgacgatt gcttcaaaac gttcaatcta cgaaacttat
    29881 gattcacaag tacaaaccaa tacagttgtc cgtccaggaa gcgatgctgc tgtgatgagg
    29941 gttagaggga cgaataaagc gttagctatg accacagact gcaacgcgcg ctatctgtat
    30001 ttggatccaa aaatcggagg acaaatagca gtggcagaag ctgctagaaa tattattgct
    30061 agcggtggta agcccttggc gatcaccgac tgcctgaact atggttctcc tgataaacca
    30121 gaagtttttt gggaattatc gacttcagct gacggaattg ctgctgcgtg tgaaacgtta
    30181 ggaactcctg ttatttcagg aaatgtctct ttatataatg aaacggatgg tcaagcaatc
    30241 tatccaacac caatgattgg tatggtcgga ttgattgagg atcataaaca tatcactact
    30301 caggaattca aaaaatcagg ggatctaatc tatatattag gtaagacgtt tgcagatttt
    30361 gatggcagtg aattacaaaa gatgcagctt ggaagaatcg aaggtgtgat ccgaaacttt
    30421 gatctatcca ttgaaaagag aaaccaagag ttagtattga cagccatcca aaacggattg
    30481 attgaaagtg ctcatgactg ttcagaaggt ggcttggcaa ttgcactggc agaatcagct
    30541 ttcaaacatc agctagggat atctgtacag tttgaattgt cctctgctca attatttgca
    30601 gaaactcaat caagatttgt gttgacagtc gcaccggaaa ataagacacg attcgaagaa
    30661 atgatgggag atgctgctgt tttagcaggt aaggtgacgg atgaagcgat aatcgaaatt
    30721 tcagcaactg acggtcagat caaaatagaa actgcagtag caagaaagtg ctgggaggat
    30781 gcaatcgtat gtcttatgaa gtgaagagtt taaatgaaga atgcgggatc ttcggtgttt
    30841 ggggtcatcc agacgccgct agtgtggcat acttcgggct ccatagtctt cagcatcgag
    30901 gacaagaagg tgcaggtatc gtgtccaatg atcgaggaaa gcttaagggg catcgagatt
    30961 tggggctgct ttctgaggta ttcaaagatc aaagaaaatt gcagcagtta acaggagaag
    31021 ctgcaatcgg acatgtacgt tatgcaacag caggcaatgg aagtgtggac aatattcagc
    31081 cttttctatt caaatttcat gatcagtcga tcggcttagc gcataatggt aacttgatca
    31141 atgctcgttc ccttagaaaa gagttagagg aaaatggggc gatcttccat tctaactccg
    31201 atacggagat attgatgcat ttgatccgac gaagtgaaga aacagatttt ttaaataaat
    31261 taaaaagtgc tttgaatgaa gtcaaaggtg gctttgccta tttattaatg acagaaacgg
    31321 caatgattgc cgctttggac ccaaatggtt ttcgtccatt agcaatcgga caaatgaaaa
    31381 acggagcata cgtgattgcc tcggaaactt gtgcattaga agttattggg gcacgtttta
    31441 taagagatgt ccagcctggt gagatagtga tcatcaatga cgaaggaatc cagattgatt
    31501 gctttacgaa ggacacgcag ctttctattt gttcgatgga atatatctat tttgccagac
    31561 cagattcaaa catcgcaggg atcaatgtgc atactgcacg gaaaaatatg ggacggaatt
    31621 tagcgaaaga atcgccagta aaagcagata tggtcgttgg cgtacctaat tcttctttgt
    31681 cagccgcaag cggttatgcg gaagctagtg gaatccctta tgaaatcgga ttagtaaaaa
    31741 atcaatacat tgcccgaaca tttatccagc caacgcctga gctaagagag caaggtgttc
    31801 gaatgaaatt atctgctgta cgaggagtag tggaaggtaa aaaggtgatt atggtcgatg
    31861 attcaattgt tcggggtacg acaagcagac ggatcgtcca tcttttgaaa gaagcagggg
    31921 caaaagaagt ccatgtgagg attgcttctc ctcctttgaa atatccatgt ttttacggga
    31981 ttgatattca aacgcgtaaa gaactcattg cggcaaatca cacgattgat gaaatcaaag
    32041 aatgtatcgg tgcagattct ttaggttttt taagtgaaga gggtctgatc gaatcgatcg
    32101 gtctggaaaa agatgctccg tattcaggat tgtgtatggc ttatttcaac ggagattatc
    32161 cgacgccgtt atatgactat gaagaaaaat atcaggaatc actaaaagaa aaagtatcgt
    32221 tcttttaaaa gcagaccaag aagggagtat atgaattcaa tggcaaatgc atacgcaaaa
    32281 gctggcgtag atgtcgaagc tggatatgaa gtagtagaac ggatcaagaa gcatgtcaaa
    32341 aaaacggaac gtttaggtat gatgggcgct ataggaggat tcggcggttg ttttgattta
    32401 tcggtctttc aattaaaaga gccagtcctt gtctcgggga ccgatggcgt ggggacgaaa
    32461 ttagcagtgg caatcgaagc agacaaacac gatacaatcg ggatcgactg cgtagcgatg
    32521 tgtgtcaatg atatcttagc acaaggcgca gagccgttat atttcttaga ctacttagca
    32581 acggggaaaa atgatccgtt acgtttggaa aatgtagtaa aaggtgtagc agatggatgt
    32641 gttcaaggaa atatggcgct gattggcgga gaaacagctg aaatgcctgg tatgtactca
    32701 ggtaatgatt atgatctagc cggatttgct gtagggatag ctgaaaaatc tcaattattg
    32761 acaggagaaa cgatcaaccc aggagatgtg ttgcttggaa tagcatctag cgggattcat
    32821 tcaaatgggt actctcttgt tcgcaaaatt ttctttgagc agcagcagtt ttctttatca
    32881 tcaaaactgc cagaattgaa aaaaacactt ggcgaagaat tgcttgagcc aacgaaaatc
    32941 tatgtttctt ccttgcttcc gctgatcaaa gaaaagaaaa taaaaggagc tgcacatatc
    33001 actggtggag ggtttgtaga aaatatcccg cgaatgttac cagaatctat ggctgcacaa
    33061 atcaaacttg gctcatggcc gattcttcca atattcggct gtttacaaaa atatggtgaa
    33121 attcctgaga tggagatgta tgaaatcttc aatatgggca tcggtatggt cctggcagtc
    33181 caagaatcga aagtaaaaga aatagtacac gaaattgagc aagctggcga aaaagtttat
    33241 gaaatcggga aagtgatcgc caaagaaaaa catcgtgtgg tgtttatagg agaagaacga
    33301 tgagaatcgc tgtttttgct tctggaaacg gaagcaactt tcaagcgcta gccgattatc
    33361 tatcgaaaaa aggattggaa tcttcgattg actggttgtt ttgtgatcag cctgaagcat
    33421 atgttttgaa acgtgcaact gcgttgagtg taccggcaga ttgtttttca cctaaagaat
    33481 ttgattcaaa aaaagagtat gaagaagcaa ttttgcataa attgaaagaa aaaaagatcg
    33541 atttgatcgt attagctgga tatatgcgaa tcattggacc ggtattgctg aaaaattatg
    33601 ataaaagaat catcaatatc catccgtctc ttttacctgc cttccctggt ttgcatggca
    33661 tacgtgatgc ctttgaagct ggagtaaaag aaacgggtgt cacaattcat tatatcgacc
    33721 aaggtgtaga tactggacca atcatccggc aggaaaaagt gagaattgaa caggaggaca
    33781 cgttcgattc tttagaagaa aaaatccata gagtagagca tcgaatctat ccagaagtga
    33841 ttagtgaaat tattgaaaat ggaggctatc tgaaatgaca agagcattga tcagtgtatc
    33901 agataaaact gggattgtag cattcgccca aggtttgatt gaacaaggag tagaaatcat
    33961 ttcaacagga ggaacaaagc atgcgttgga taaagctggg atcaaaacgc tttcgatcga
    34021 agaagtgaca gggtttcctg aaatgatgga tggccgtgtc aaaacacttc atcctaaaat
    34081 ccatggtggt cttttaggaa gacgcgatct ccctagccat atggaggcaa tggaaacgga
    34141 aaatatccaa ccaatcgact ttgtttgtgt caatttatat cctttcaaag aaacgattat
    34201 gaaaccagat gtcgaaatag cagaagctat tgaaaacatt gatatcggtg gtcctagtat
    34261 gctccgtagt gcagcaaaaa accatgaatt tgtcacctct gttgttgatc catctgacta
    34321 tgaagaagta ctttcagaat tacgtcagta tgggcatact tcactttctt tcagacaaaa
    34381 actagcggca aaagtctttc gtcatactgc ggcatatgat gcactgatcg ctcaatactt
    34441 aacggattgg gtggaagaaa aagaaccgga aaaattgaca cttacgtatg aacgaaaaca
    34501 aacgcttcgt tacggtgaaa atagccatca agccgctgcc ttttatcagt ccgctcttct
    34561 ggaaaaatat tcgatcgcag cagcaaaaca actccatggg aaagaacttt cttataataa
    34621 tatccgagat gcagatgctg cgctacggat catgaaggaa tatgacgagc caacggtagt
    34681 cgccttgaaa cacatgaatc cttgtggtat tgggacagca tctacgattt tagaagcgtg
    34741 ggaagcagcg tatgaagcag atcctgtttc aatctttggt gggatcatcg ttttgaatcg
    34801 agaagtagat ctgccaacag cagaacagat gcataagctg tttctagaaa tcatcattgc
    34861 cccttcttat acacaagaag ctttggaatt actgcaaaaa aagaaaaaca tccgattact
    34921 gacagtagac tttgcgcagg aagaaaatca aaatcgagaa gaaacggtct cagtcttagg
    34981 tggacttctt gtacaagatc aagatctggc aatagaaaat gaagaagagt ggaaagtcgt
    35041 gacaaaacgt aagccgactc cagcagaagc aaaagcatta gcgtttgctt ggagagctgt
    35101 caaacatgtg aaatctaatg cgatcgtact ggcaaacgaa cgtcagacag taggaatcgg
    35161 agccgggcag atgaatcgag tcggttcagt aaaaattgcg attgaacaag cgcaagagaa
    35221 aatggaaggt gcagttttag ctagcgatgc ttattttcca atggatgatt cggttgaata
    35281 tgcagccaaa catggtataa aagcaatcat tcagccagga ggaagcatca aagaccaaga
    35341 ttctattgat atggcgaata aatacgatct agcaatggtc tttactgatg ttcggcattt
    35401 tagacattag agaggagtcg gataaatgaa cattttggtg atcggtgcag gtggtcgtga
    35461 acatgcaata agtaaaaaac ttttagccag tccgaaggtg gatacggtgt attgtgcgcc
    35521 aggaaatccg gggatgacaa aagatggtat caattgtgtc gctatcagtg agcgagatca
    35581 ttcagcgtta atccattttg ttaagaaaga ggctattgat tggagtatcg ttggtcctga
    35641 agttcctttg ttaaatggga tcgtcgatga ttttatggcg gaaggattga aaatcttcgg
    35701 tccgacaaaa gcagcagctc tgatcgaagg ttcgaaaagt tttgctaaac aattaatgga
    35761 tacttatcaa atcccgacag ctaaatccag aagttttgaa tgttacgaaa aagcagcagc
    35821 gtatatcaaa gaaaatggtg cgccaatcgt agtgaaagcg gacggcttag cagctgggaa
    35881 aggtgtagtc gtggctacca cagtgaagga agcgctggat gcggcagaac aaatgttgaa
    35941 ccagcatcga tttggggata gtagcaaaaa aatcgtcgta gaagagtttt tggcaggaga
    36001 agaattctcc cttttggcgt ttgtccgtaa taccaaggtg tatccgatgg tcatttcgca
    36061 agatcataag cgggcatttg ataacgataa agggccaaat acaggcggaa tgggtgctta
    36121 ttcaccagta ccgcaaattt caaaaaagat gattgaaaaa gctgtggaag aagttttaaa
    36181 accagcggct ttgggaatga aagaactagg acgctctttc acgggtgttt tatatgcggg
    36241 tttgattgct acgaaggaag gaccaaaagt gatcgaattc aatgcgcgtt tcggtgatcc
    36301 tgaaacgcaa gtcgttcttt ctcggttgaa gagtgacttt gcgcaagtaa tcgatgattt
    36361 attagagaat cgtacagtag agttaaagtg gcagcaagaa ggatacaatg taggcgttgt
    36421 ggttgcagct gccggttacc ccgaagaata tgagacagga atggttctac cagattttct
    36481 ggaagaagag ctttctgttt actatgctgg agtttcctca aataacggca aactagttgg
    36541 agcaggagga cgtctttatt tagtagaagc atacggtgag acgatcaaag aagcacagaa
    36601 aaaaatttac accgcgttaa tggataaaac gacagatgga acattttatc gtacagatat
    36661 tggagaaaaa gctgccaata gcgaactgat cgtgaagtaa atcagctgct tccatttata
    36721 aaaaatacaa caaacataat cggaagcgcc gattttagtg aagccttttc ataaagagtg
    36781 tggtacactt ttgttgtgta tataaataag gaggagttca gatgaaaaaa attcaattag
    36841 gtacttctga tcagaaagca gctgcagtga tcttaggctg tatgcgtatc aacaatgcgg
    36901 aagatccagt aaaagtgatt gagacagctt atgaaaacgg cattgatttt tttgaccatg
    36961 cagatattta tggaaacggt gaatgtgaag aaatctttgc agatgctcta gcaaaaacat
    37021 caatcaaacg agaagatctt tttattcaaa caaaatgtgg aatcgttcca ggtgtgatgt
    37081 ttgatttttc taaaaaacat atcattgaat ctgtagaaaa aagtttgacc cgtctgaaaa
    37141 tggattatgt ggatgcttta cttcttcatc gtccagatac attggtagaa ccagaggaag
    37201 tagctgaagc gtttgatgaa ttggaacggt caggaaaagt tcgttatttt ggcgtcagca
    37261 atcaaaaacc aatgcaaatc gaattgctga aaaaatatgt tcgtcaacca ttagtagcta
    37321 atcagctgca atttggcttg aaacatactg gaatgatcga tcaagggatt catgtcaata
    37381 tgacagatga agcaagtatc gatcatgacg gtagtgtact tgattattcc cgattgcatg
    37441 atatgacgat ccaagcatgg tcaccatacc agtatggatt ttttgaaggt gtatttatcg
    37501 ggaacgaaaa attccctgaa ttaaaccaaa cattagaaga actagcagct aaatatcaaa
    37561 cgacaccaac tggcttggct tctgcttgga ttttacgcca tccagccaat atgcaagtga
    37621 tcgccggtac gatgaacgta gggcgaatca aggaaatcgc tcaagcttca gaaattaata
    37681 tgtctcggga agattggtac gaattgtacc gtgctgctgg aaacatttta ccttaaagat
    37741 tgtcataagc aggtcaaaag ggctataaat taaaaagaca gaataacttg aaagctgttt
    37801 tcagcttcga gatattctgt ctttttttat tgcaaatgga aagagtatca ttgtacggat
    37861 ttcgtttatt agttattttt gtttttaagg aaaagctgtg tccgatattc ttttggagaa
    37921 atcccttgtt ttttcttaaa taaataactg aaatagttag ggtcattata accaatttca
    37981 aaagcaatat ctgaaatacg atagtctgat tgctgcaata atttttttgc taattcgatt
    38041 ctttgttctg tcagataatc gatgaatgtt tggttaaacg attgagaaaa aatcgtactg
    38101 aaatgagagg gacttaatgc aacttgttga gcgacccaag ttaaggacat atctggatcg
    38161 gtaaaatttt ctttgataaa atccaatgca tggttcaaaa ttggctgata tttgttcctt
    38221 gaaggctgtg agggcagagc agttaagaaa gaaatgaaca tataaactgt cgaacggtaa
    38281 agcgagtcgt cggcgctgat tgctaagcgt tgactggtgg atgtgcaatg aaccatttct
    38341 tgcgggattt tcttttcttt caggcagaca tgctggagta gcttagtcaa gacaaataaa
    38401 cgatataatc gtgttcgagt tggtgtgcct tgtggtttcg tcaagtgttc gatgtactta
    38461 gatacttcgt ttttcgataa tgaagacaaa tcaagtgaga ggtcaaagta aaagacagaa
    38521 tcaactggaa gtttcgcttc ttgaatcaaa tcctcatagc tgataatttg ttctttaagg
    38581 gaactggcgg tttcaagtat catctgcgtg atctcaaaag atgcaggtaa ttcgctgatt
    38641 ctttctgcga caggaccaat ggcgacagaa aaattatttt tttgtccctt ttctaattca
    38701 tggaccaacg tttgggctac ctgataactg gtctcgatca cattttcttc aagagaatca
    38761 actattaaaa atttgatgaa tcgagaggaa acagcagaga agatcacttg ttgattctcc
    38821 ctaaataaaa acttcaaata atcactgaga tgagcgtaat cttcaaagtc tgtactgact
    38881 tggtttgctg ctaataatac ggtcattttt ttccctgcta aagagcgatt gaaagattca
    38941 gcaagtttca atgtttcact gatcgtaagt gagcctttga acaaaccgtt caagaaatga
    39001 ttttttttca attcgaaaac aagatcagaa gaagtgtgcg tttctttttg ctgcaaatct
    39061 aaagcagtta ctgctttttc tattgcttgt ttcaattcgc tacttttaat cggtttaagc
    39121 agataatcat ctgcctgtac ctggattgct cctttcatat aatcgaaatc gtcgaatcca
    39181 ctaataaata tgagacgagt ccaaggaagt agtttccttg cttcttttga aaaagtcaac
    39241 ccgtccatga aaggcatttg gatatcagtc agaatgatgt cgggctgtaa gtcgataatt
    39301 gaagacagtg ccatctcacc atctccagct tcgccgacat aagtgattgg ataataagaa
    39361 ccaaggacag ttatttgttc ccgcaagtta ttgcgaatga ggtattcgtc ttcgatgata
    39421 aaaactttat acatgtttgt ttcctccaat tgttttggga gatagaggga tcatcagtgt
    39481 gacagttgtt ccttctcggt atttgctgtc aatcgtgata ggtttttgat catttccgta
    39541 ataaagaagg agacgcttgt ttacgttgta aagtccgtag ccgatactga aatctgaatc
    39601 gatccctttg gctaactctt tacgtatttc tgccagtttt tctgcaggga tgccaatccc
    39661 gttgtctata actttgaatt ggatcatctc atcaattttg catgtggtaa tacgaataaa
    39721 tcctttgtga cggacgtact tcgttccatg gtatacagca ttttcaatca atggctgcaa
    39781 gatcattttt aaaatagaaa caggaaaaag tgtttctggt atatcgatct tatagtcaag
    39841 catatggccg tatcttattt ttaagatgac tagataatca ttgatatggc taatttcttt
    39901 ttcaagagga atccagtctt gtcctttgct taaagacgta cgaaaaaagc cggaaagtgc
    39961 atgggtcata gtcagagctt cttcaaagca gtcttgttcg atcaaagaaa taatggcatc
    40021 caaactgtta taaataaaat ggggagtaat ctgagcttgc aatacacgaa cttcactttg
    40081 tgctaaatgg tattgtttca aagcgttctc ccgtaaagac tggtttagat cttccgccat
    40141 tttattcatg ctagcggtaa gtgtttgtaa ttccagtgtt tccggcaggg ttaaacgaaa
    40201 gccaaggttc cctgaagaaa gttgttctga catctcgatc agctgattga tcggttcttg
    40261 gacctttctc tctaaaaagc gacgattctt tttcgcatag aaaaaaatgg tactaagaac
    40321 tccaagttcc actattgtta atagaatgag cgagcggaca agtgtcgtat tttttttgct
    40381 tgctaagtcg atctccacac gaacaaaatc ttgtaagata tctgacagca gttgagtgac
    40441 agaatctacc tgattgatga tctcttcatt ggtatccata aaatcttttg ccaagatatt
    40501 attgatcagc tcgttttgat aatcttccag tgtatcgatc gtacgtagcg atacatctaa
    40561 cgtactaatt tctttaagtg tatttgtatt atttttgatc tctcgaatat cattcctaag
    40621 ttcttctagg atattttcct cattgaactc atctacggtt acttggccag aaatcaagtc
    40681 ccacatactt tccaaaactt tttgatcaac cttcaaagca attgcatctg ctttttggat
    40741 atttgcgatc gtctgttgat aaatcaaaag ctgttgcgaa tataacagta tgctcagtag
    40801 caaagggata acagcaaata gaagcatggc tttgtttgct tgggcaaaaa tctgttttat
    40861 gcttaaattt tttgtcatga tacttctcca gtcaataatt tctggttggg atgttagcta
    40921 tattctgctg ggaaaagaca gtctcggaaa taaaaatatc tttaggtaat gtttttcctg
    40981 tttgaatatt tccaaaataa cgagtaacgg tattggctac ataccagccg acatcaggtt
    41041 tacactcgac tacacaattg acgattcctg agcgtagctt ctcgatcata tcttcttgtc
    41101 catcaatagt tacaatgatc aaatcttctc caggtttgat tgatgtttct tttatcgcag
    41161 ctaaagcacc gtgagtcatt tcatcactat ggctgtataa tacatcgatt tcggataaat
    41221 catttgtttc taaatatcgt ttcatctggt cttttccttt catccgaata aaatcaccga
    41281 caattcggtg aacaatagtc atcggaggtt tatggtcgat ggttccatct ttttttaggg
    41341 ttggtttacg attgattgat tccaaaaatc cttcggagcg tagcttggtc ggtgtggagt
    41401 tctctgaacc agcaagttct aggattctga tttcctgttt ctttgaattt gtgaagtgat
    41461 tgctgacaaa taaacccgcc cgatttcctt ctgctttgaa actggggcca atgtgggtga
    41521 cgaaaagctc gggatcattc gtttgaatat gtctgtcgac aagtatgaca gggatccctg
    41581 ctcgctttgc ttcttttaat acagagtccc acccggattc ttgcaaagga gtaaaaatga
    41641 tcatatccac tttataggca atgaacgtcc gtatatcttg gatttgccgt tcctgattca
    41701 tgtagccatt tcggtagaga acttggtaat cttctttttc aagcgcagtg ataattgatt
    41761 cagtatgctt tttccgccag ctactctccg ttcctgattg cgaaaaacca acgactagtg
    41821 ggtcaggtgc ttcctttgta ttaggtgtac accccatcat ttggtaagcg attacaacaa
    41881 gggctaaaaa aaataaaaca cgtttcactt taaatcactc catctggatt tgttagtact
    41941 atcttatcag ataaaaggga aaatcaaagt caaaaggaat caatcgaaaa aaatccaaga
    42001 aacttaaaaa aatccaagac aaaaaaagta cacctaaaaa atttaaagta aatcccataa
    42061 aaaaatcaat gttaacgctt tctttattag ctatactcgg tgtatcaatt gaaatggggg
    42121 ataaaaaatg aaaaacaaat ttagaaagat tgttgggact atcgctattt tgaccgcaag
    42181 cattctatta agtgcgtgtg gcaattcagg atctgcagat gatagtacgg ggtatgtagg
    42241 aatcgctatg ccgaccaaat cagcagaaag atggatcgct gatgggaaca acatggtctc
    42301 ggaattagaa aaactgggtt ataaaacaga tcttcaatat ggagaagata aagtagaaaa
    42361 tcaagtagca caaatagaaa atatgattac aaaaggggta gatacacttg tgattgcttc
    42421 tatcgatggt tcggctttaa ctgatgtgtt agctaaagca aaagaagcag atatcaaggt
    42481 gatcgcctat gatcgtttac tgatgaattc agaaaatgtc gattattacg cgacttttga
    42541 taattttgga gtaggtgtat cgcaagcagc ttatattgaa gagcacttag gattgaaaga
    42601 aggaaaagga ccattcacga ttgaattatt tggtggctcg cctgacgaca acaacgcact
    42661 aatcaattac aacggagtga tgtctgtttt acaaccatat atggacaacg gtcagttggt
    42721 cgtgccatct ggacagacga gtttcagtca aatcgctacg cttcgctggg atggttctac
    42781 ggcgcaagca aggatggata atctgttaag cgcaaattat actgaccaaa cattggatgc
    42841 tgttttgtcg ccatatgatc caataagttt aggaatcatt tcgtcattaa aaggcgtggg
    42901 gtatgggagt gaaagtaaac ctttacctgt gattactgga caagatgcga cagttgctgg
    42961 cgttaaatcg atcattgccg gggaacaaac acaaacgatc tttaaagaca cacgtatctt
    43021 agcaaaaaat acgattgaaa tgatcaaagc aatcagtgat ggagaagagg ttccggtcaa
    43081 tgatactgaa acttatgata atggggtgaa aactgtccct acgtatttag caaatacagt
    43141 atctgtcgat aaagataatt atcaagcaga gctgatcgac accgattatt ataaagaatc
    43201 cgatctgaaa aattaagagg aaccaaacaa ggaactaaag agatctaagt cagagagagg
    43261 ataagaatta tggcggattg tattttaaaa atggcagata tcaccaaaaa attttctgga
    43321 atccgagcgt tagattgcgt gaatctgatg attgaacgtg gggaaatcca tgcattgtgc
    43381 ggagaaaacg gagcgggaaa atcaacattg atgaatgtct tgagcggctt gtatccatat
    43441 ggctcctatg acggggaaat cttttatgat ggaaagctgt gtcaatttaa agatttgaaa
    43501 gatagtgaag aaaaaggcat cgtcatcatt catcaagaat tagcactaag tccttatctt
    43561 tcaataaaag agaatatttt tctcggaaat gaacaagcag aaagaggcgt gattgattgg
    43621 gaactgacag aaaagaaaac atatgaactg ctgaataaag tcggtttgtc tatcgacccg
    43681 aatcttctag tatcccagat tggcgtaggg caacagcaac tagttgaaat agctaaagca
    43741 ttatcaaaat ccgtccgtct gttgattttg gatgagccta ccgctgcatt gaatgaagaa
    43801 gaaagcgcga atttgctgag attgatccga gagttgaaag agcaaggtgt aacctccatc
    43861 attatttcac ataagctgaa tgaaatcgtg gatatcgcag atcgaatcac cattttacgg
    43921 gatggacaga cgattgagac actagaaaat gaaatgatca gcgaagagca tattattcgc
    43981 ggaatggtgg gcagagattt agttgatcgt tatcctgaaa gaaaaccgga tattggagaa
    44041 gtttatttta aagtagaaga ctggacggtc tatcaccctc ttgatcacga gcgaatcata
    44101 aacgaccaca tcaacttgca tttgaaaaaa ggagagattg taggaatagc tggactgatg
    44161 ggagcgggca gaactgaatt cgctatgagt ctatttggac attcttatgg aagcaagata
    44221 tccggacgta tattaaagga aggggcagag atatcagtta agaccgttcc ttcagccatt
    44281 gcaaacggac tcgcttatgt ctcggaggac cgaaaatctt taggactgaa tctgctgatg
    44341 gatattcggg aaaacgtgac gctgagcagt ttggataaaa taagtcaaaa aggagtatta
    44401 gataaagaaa aagaagtaac tgaggcagaa aatttccgga aaaagatgcg tataaaaaca
    44461 aactccatcc atcaaattgt cagtagtctt agtggtggga atcaacaaaa agtcgtttta
    44521 tccaagtggc tgatgacaca acccgatatt ctttttttgg atgagccaac ccgtgggata
    44581 gatgttgggg caaaatatga gatctacacg attatcgaag aaatggcagc aaatggcaaa
    44641 gctgtctgtc ttatctcttc tgaacttcct gaaatattag ggatgtgtga tcgaatctat
    44701 acgatgaacg aaggacgtat tacaggagaa gtctcgcgag aagatgcaga tcaggaagtc
    44761 ttgatgaaat taatgacgaa agaagaggtg ggttaagttg gaacaggcaa aaaatcttac
    44821 ggataaaaaa gagaaaaaac taaatttgaa aagtagagca gctgatcttt ttggtcgcta
    44881 cagcatggtg atcattttag cgggactact tattgcgttt caattgatga cggatggtat
    44941 tttctggcgt ccattgaata taactaacct tgttctgcaa aatagccata tattagtact
    45001 agctgcaggg atgttattag ttgtgctctt aggtcatgtg gatttgtcgg tagggtcagt
    45061 gatggcgttt gttggtgcaa ttgcgggcat gatgatggtc aatcgccaaa tgaacccgtg
    45121 gatgacagtt atcgcatgtc ttgcagtagg cgggctgatc ggcgcatggc aaggtttttg
    45181 ggtagcttat gcagggatac cggcgtttat cgtgacttta gcaggattgc tgatgtttcg
    45241 tggactgaca caagttgttc taggaggcca atctctagca ccttttccag ctgttttcca
    45301 aaaaatgtct acgggttatt taccagactt aagcgaagga agcagccttc acttcttgac
    45361 attgatttta ggaatgatca tcacgatcgt gttagttgtt ggtcaatggc gaacaagaaa
    45421 tcgtcggaaa aatcatttat ttgaagtgga atctccaaca gctttcttgg taaaggcggt
    45481 atttacagca gtcgttgtac taggaataac ttatatcttt gcctcttatc aagggtatcc
    45541 gattattttg atcattcttg gtttgatcgt tgcagcatat gggtttttga caaataaaac
    45601 agtagcaggt cgacagattt atgcgacggg cggaaatcga aaagcggctg aactatcagg
    45661 gatcaaaaca aagcgaatca cgttctgggt attcgtcaac atgggagtca tggcagcttt
    45721 agctggatta gttttagctg ctcgattgaa tgcggcaaca cctcaagcag gtacatcgat
    45781 ggaactggat gcaatggcgg cagtatattt tggtggtgct tcgacttccg gcggcattgg
    45841 aacaatcatg ggtgcgattg tcggtggctt ggtcatgggt gtattgaaca atggcatgtc
    45901 gattttaggc gtcggggtag actggcagca ggcaatcaaa ggattgatct tgcttttagc
    45961 cgttgtgcta gacttgtata ataagaaaaa acgaacaacg taggagaacg ccggttattc
    46021 agaaaaaaga ctgtgacaag acatttgtca cagtcttttt ttcgctaaaa gcgaacattc
    46081 attgtttcgt ttgt
//
LOCUS       DAZKOW010000010        45523 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000010 DAZKOW010000000
VERSION     DAZKOW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.010"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(94..1729)
                     /locus_tag="ACG912_000577"
                     /pseudo
     CDS             complement(94..1729)
                     /locus_tag="ACG912_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2052..2531)
                     /gene="rlmH"
                     /locus_tag="ACG912_000578"
     CDS             complement(2052..2531)
                     /gene="rlmH"
                     /locus_tag="ACG912_000578"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFQ0511923.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            2959..4248
                     /locus_tag="ACG912_000579"
     CDS             2959..4248
                     /locus_tag="ACG912_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFQ0511924.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(4287..4892)
                     /gene="ytpR"
                     /locus_tag="ACG912_000580"
     CDS             complement(4287..4892)
                     /gene="ytpR"
                     /locus_tag="ACG912_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFQ0511925.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(5097..5561)
                     /locus_tag="ACG912_000581"
     CDS             complement(5097..5561)
                     /locus_tag="ACG912_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0511926.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(5583..5903)
                     /locus_tag="ACG912_000582"
     CDS             complement(5583..5903)
                     /locus_tag="ACG912_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0511927.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(6047..7126)
                     /gene="pepA"
                     /locus_tag="ACG912_000583"
     CDS             complement(6047..7126)
                     /gene="pepA"
                     /locus_tag="ACG912_000583"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFQ0511928.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            7326..7676
                     /locus_tag="ACG912_000584"
     CDS             7326..7676
                     /locus_tag="ACG912_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0511929.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            7899..8846
                     /locus_tag="ACG912_000585"
     CDS             7899..8846
                     /locus_tag="ACG912_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0511930.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(8903..10321)
                     /gene="pepV"
                     /locus_tag="ACG912_000586"
     CDS             complement(8903..10321)
                     /gene="pepV"
                     /locus_tag="ACG912_000586"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFQ0511931.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            10470..11315
                     /locus_tag="ACG912_000587"
     CDS             10470..11315
                     /locus_tag="ACG912_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFQ0511932.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            11319..11759
                     /locus_tag="ACG912_000588"
     CDS             11319..11759
                     /locus_tag="ACG912_000588"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFQ0511933.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            11809..12003
                     /locus_tag="ACG912_000589"
     CDS             11809..12003
                     /locus_tag="ACG912_000589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511934.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            12139..12825
                     /locus_tag="ACG912_000590"
     CDS             12139..12825
                     /locus_tag="ACG912_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0511935.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IQEALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(13233..13922)
                     /locus_tag="ACG912_000591"
     CDS             complement(13233..13922)
                     /locus_tag="ACG912_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511936.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(13897..16026)
                     /locus_tag="ACG912_000592"
     CDS             complement(13897..16026)
                     /locus_tag="ACG912_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFQ0511937.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(16074..17411)
                     /locus_tag="ACG912_000593"
     CDS             complement(16074..17411)
                     /locus_tag="ACG912_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFQ0511938.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(17663..18022)
                     /gene="rplT"
                     /locus_tag="ACG912_000594"
     CDS             complement(17663..18022)
                     /gene="rplT"
                     /locus_tag="ACG912_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFQ0511939.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(18075..18275)
                     /gene="rpmI"
                     /locus_tag="ACG912_000595"
     CDS             complement(18075..18275)
                     /gene="rpmI"
                     /locus_tag="ACG912_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFQ0511940.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(18347..18871)
                     /gene="infC"
                     /locus_tag="ACG912_000596"
     CDS             complement(18347..18871)
                     /gene="infC"
                     /locus_tag="ACG912_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFQ0511941.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(18915..19040)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            19191..19823
                     /locus_tag="ACG912_000597"
     CDS             19191..19823
                     /locus_tag="ACG912_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0511942.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(19825..20730)
                     /locus_tag="ACG912_000598"
     CDS             complement(19825..20730)
                     /locus_tag="ACG912_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0511943.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(20815..21474)
                     /locus_tag="ACG912_000599"
     CDS             complement(20815..21474)
                     /locus_tag="ACG912_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0511944.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            21790..22734
                     /gene="mvk"
                     /locus_tag="ACG912_000600"
     CDS             21790..22734
                     /gene="mvk"
                     /locus_tag="ACG912_000600"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFQ0511945.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            22727..23704
                     /gene="mvaD"
                     /locus_tag="ACG912_000601"
     CDS             22727..23704
                     /gene="mvaD"
                     /locus_tag="ACG912_000601"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFQ0511946.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            23717..24802
                     /locus_tag="ACG912_000602"
     CDS             23717..24802
                     /locus_tag="ACG912_000602"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFQ0511947.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            24799..25863
                     /gene="fni"
                     /locus_tag="ACG912_000603"
     CDS             24799..25863
                     /gene="fni"
                     /locus_tag="ACG912_000603"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFQ0511948.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(26112..26915)
                     /locus_tag="ACG912_000604"
     CDS             complement(26112..26915)
                     /locus_tag="ACG912_000604"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFQ0511949.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(26935..27777)
                     /gene="kduI"
                     /locus_tag="ACG912_000605"
     CDS             complement(26935..27777)
                     /gene="kduI"
                     /locus_tag="ACG912_000605"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFQ0511950.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(27793..28800)
                     /locus_tag="ACG912_000606"
     CDS             complement(27793..28800)
                     /locus_tag="ACG912_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFQ0511951.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(28807..29451)
                     /locus_tag="ACG912_000607"
     CDS             complement(28807..29451)
                     /locus_tag="ACG912_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFQ0511952.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(29551..30309)
                     /locus_tag="ACG912_000608"
     CDS             complement(29551..30309)
                     /locus_tag="ACG912_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFQ0511953.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(30436..31587)
                     /gene="fucO"
                     /locus_tag="ACG912_000609"
     CDS             complement(30436..31587)
                     /gene="fucO"
                     /locus_tag="ACG912_000609"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFQ0511954.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(31669..31980)
                     /gene="rhaM"
                     /locus_tag="ACG912_000610"
     CDS             complement(31669..31980)
                     /gene="rhaM"
                     /locus_tag="ACG912_000610"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFQ0511955.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(31982..32827)
                     /gene="rhaD"
                     /locus_tag="ACG912_000611"
     CDS             complement(31982..32827)
                     /gene="rhaD"
                     /locus_tag="ACG912_000611"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFQ0511956.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(32841..34097)
                     /gene="rhaA"
                     /locus_tag="ACG912_000612"
     CDS             complement(32841..34097)
                     /gene="rhaA"
                     /locus_tag="ACG912_000612"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFQ0511957.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(34094..35554)
                     /gene="rhaB"
                     /locus_tag="ACG912_000613"
     CDS             complement(34094..35554)
                     /gene="rhaB"
                     /locus_tag="ACG912_000613"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFQ0511958.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            35707..36576
                     /locus_tag="ACG912_000614"
     CDS             35707..36576
                     /locus_tag="ACG912_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0511959.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(36702..37601)
                     /locus_tag="ACG912_000615"
     CDS             complement(36702..37601)
                     /locus_tag="ACG912_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0511960.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(37766..38590)
                     /locus_tag="ACG912_000616"
     CDS             complement(37766..38590)
                     /locus_tag="ACG912_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0511961.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(38601..39896)
                     /locus_tag="ACG912_000617"
     CDS             complement(38601..39896)
                     /locus_tag="ACG912_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFQ0511962.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(39912..40391)
                     /locus_tag="ACG912_000618"
     CDS             complement(39912..40391)
                     /locus_tag="ACG912_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFQ0511963.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(40397..41425)
                     /locus_tag="ACG912_000619"
     CDS             complement(40397..41425)
                     /locus_tag="ACG912_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFQ0511964.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(41442..42548)
                     /locus_tag="ACG912_000620"
     CDS             complement(41442..42548)
                     /locus_tag="ACG912_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0511965.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(42777..45374)
                     /gene="adhE"
                     /locus_tag="ACG912_000621"
                     /gene_synonym="adhC"
     CDS             complement(42777..45374)
                     /gene="adhE"
                     /locus_tag="ACG912_000621"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFQ0511966.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT        13177 a         9673 c         8258 g        14415 t
ORIGIN      
        1 gattaagctt cattattata atataaagtt taaatttggt ttattactta ctttttctta
       61 ttctctagta agctaatatt gaaaaaaatg taatcagatc attattttta tatgttgtaa
      121 cttcctgata agaaaactaa ttaaaagaaa gaggtaacta cataaactaa ttaaaaaagc
      181 taacttcata gttggaggat aaatggaaga aacttcaatg attgtttctc cttgtgttcc
      241 ttcaatcgac actagtccta ttatcaatct ctcttttttg tccatttact tttgcgactg
      301 ttccccaata atcaaataca ggaagcgtta ttttttgtcc tttagattca tgtactgtat
      361 agactatttt cgaatccatc acttgataag gcatagtatg ccattccttg ccaattctta
      421 cttcatgatt ctgcaaatgt tctttgtatt gtaatgcttc cgtgggtgca tagtcataaa
      481 gtcctccggg aaggagtgtc gtgtaacgaa taagattttc ttgattcatt accaagtgat
      541 ctctctgagt gacaacttta tgttcatagt ttgcttttgt tgcagcagat acagccaaca
      601 gtaataaaac aattgccgtt atattaacta cttttctttg cttagctgat gtgctcttca
      661 tgaaaacgaa cgataaaatc aacgagccaa acagtacttg aaagcctaag attcgccaag
      721 gaaattgaat catttgaatg ggtgtatttt gtaataaaga ccatggaaaa aatttggtgg
      781 ataagagcca agagactacc gcactaaggt aaatcacttt ttctttgcga ttaaatcctt
      841 taaaaaagaa gagtggacag atcaaagaaa taataccaat gattcctacc gtaaaggttg
      901 tcaggtcatt attcatcgca ccaatcaaac tatccgagac atttaatgct tggtgttgta
      961 aggtcgtttt atctggctca ttaatcgatt gaaccatgat ttgtcggaac atcggatacc
     1021 aaaagtatgc tgttaaaaga atcgtcattc ctactgcctt tgacaactcc acaatcaacc
     1081 ggatgctttc tttttttctt gtttcactta ctacaaagcc gcctaagact agtaagccaa
     1141 cggctattac actcataaaa gtggaaagaa catgtgtata aatgagtaag ctcatactga
     1201 ctgctaatgg aattgcacat ggctttttct cataaatcag tgcgtaagca taatacataa
     1261 ctatcggtat aaccgtaagt gcaatcgctt cagcaatagc agaacgatga taaatatcaa
     1321 tcgtacggta cccataaaat gtatataaac agctaaatat aaaagccgca tcgattcttt
     1381 ttaaaaattt tagtccataa tgaaacatca gcatgatagt acaaacattc aatagccaaa
     1441 catataagat ataactaacg attaaattat tacttatcca ataaaaaatg actgccggaa
     1501 aaaaggtcaa gtagggataa aaataattca ctccgctccc ataatgatta aacgtcgtaa
     1561 aattgattgg accagaaaaa attgaagata accctttgac tctagcaaaa tggaaggcaa
     1621 tatcccccga tgtagaaata gtatgaacat ttctaaaata attgattgaa taaagtccag
     1681 caataattgc taggagaatg gacaatatta tttttttctt tacttgcatt acttaattat
     1741 atactccttt catggattgc tttatctagt ataacttaaa ttttaaataa atcgttattt
     1801 atttttactc tcacttaaaa aagcactaaa attataagag agggaggtaa tggtctgaac
     1861 accaagaatt gttccaacat cgaattgctt aactaatgag ttcaaagctt aattatgtgg
     1921 ctcttatatt taccgagcgt ggattgcgat ctctttagcg aaacaaacat ctttcataaa
     1981 aaaaacagcc atatccgctt tcttggatat gactatttac tactacttta ctatctttca
     2041 ttcttcttgt ctcacttatg ataaggcgcc cccgcattga tccggaaggc acggtagatt
     2101 tgctcgacaa ggatcagacg catcaattga tgtggatacg tcatttttcc aaaagagatc
     2161 tgagcattgc ttcttttcat gacggcttcg ctgagtccta aagagccgcc gataacaaaa
     2221 acaaggtggc tttttccttg gatacctaat tggtcgattt gtttagcaaa agattcactt
     2281 gtaggatttt tcccttcaat tgctaaggca tagacgtatt cttgttcttt gattttcgct
     2341 aagatccgtt ccccttcttt ttcttttact tgtctcattt gcgcttcgct taaattctcc
     2401 ggggcttttt cgtcaggaac ttcgatcaat tcgatttttg cataggcatt cagccgtttg
     2461 acatactcat tgataccttg gatcaggtat ttttctttta attttcctac tgagataatt
     2521 ttgatattca tgttttctct cctttggtct tcatgctcac tttgtttatt ataatcaatt
     2581 ttttcgtcta tatgtttatt ttggctattt ttgttaaaaa gatttcccca acagttatgc
     2641 acaacatcca caggtttatc cacagaaaac agtgttattt taccttattt catcacattc
     2701 attaggcaaa catacgtttt ataagaattt atccacttta tacacaggct gtggatatgt
     2761 tttatccctt atttttccaa tcctatccgc gtagttttac acagtatctg tggaaaaaga
     2821 aaatcaaata ttttcatctg aaaacaatga tttttcctaa agtttttcat aaaaagttca
     2881 cagtctcaaa gtagactaaa gtcaacaaat aagactatac taacagtaga taaatgaatg
     2941 taattggagg aggaacacat ggatagaaaa aatgtgacac caaagatgaa aaaaagtaaa
     3001 aataatagcc tctggcgtaa gttaggtctg gggcttgttg gagggatcgt tggaggacta
     3061 ctgacagctg gcatttttta tgcagcgatg ggcactggca acaatagttc tactgctaca
     3121 agcggcaatc agaattctgc cggtgaaaca gttgtggaaa atgtcaaagt caatgtagat
     3181 tctgatatca ccaaagcggt agataaagtt caagacgctg tcgtttctgt tatcaactta
     3241 caaagtcaaa accaatcaag cggttttggc ggactattcg ggcagcaaga agaaagtagc
     3301 agctcagatg atagtaattt ggaagcttat agtgaaggaa gcggcgtgat ctacaaaaaa
     3361 gacgggaata ctgcttatgt agtcacgaat aatcacgtag tagatggcca gcaaggtctt
     3421 gaagtcttga tgaaagacgg tacgaaggta aaagctgaat tagttggaac agatgcttat
     3481 tcagatttag cggtgttgaa gatcaattca gacaaagtcg aaacagttgc ctcttttggt
     3541 gactcaagtg ctctgaaggt tggtgagcct gcgattgcga ttggttcccc actaggatct
     3601 gaatatgcaa attctgtcac ttcaggaatc atttcttcct tgaaccgaca agtgacaagt
     3661 acgaatgaat cgaacgaaac agtcaatatc aatgcgatcc aaacagatgc agcaatcaac
     3721 cctggaaatt ctggtggtcc tttagttaat atcgaaggac aagttatcgg tatcaactca
     3781 agtaagatcg caagtacttc tgaatctact tctaatgtaa gcgtagaagg aatgggattt
     3841 gctattccaa gtaacgatgt agtgaatatc atcaatcaac tggaaaaaga cggaaaagtg
     3901 acacgtccag ctctaggtat cacaatggtc gacttatctg ccgtttctac tcagcaacaa
     3961 gaacaaatct tgaagattcc ttcatctgta actaacggtg tcatcgtgac aaatgtccaa
     4021 acagctacac cggctgaaaa agctggattg aaacaatacg acgtcattac taagatcgat
     4081 gacaaagaag tttcttctgg tgtagaactt caatctgctt tgtataagaa aaaagtggga
     4141 gattctgtca aaatcactta ctatgaaggc tcgaaagaaa aaacaactac tgttgaattg
     4201 acgatcgatc aatcggcatt aaaacaaaat caaaatagtg aaaattaata aaaaagaggt
     4261 tgtgacaaag acttgtcaca acctctttat tttggaaatg cttctccgac tacggcatcc
     4321 tcaggcaata ctaagattcc tttttttgct ggagcatttg gcaaatgcaa ttcacgagcg
     4381 gaacagatca ttccatagct atctacccct cgcaaagttc ctggccagat catcattcca
     4441 tctggcatca tagctcctgg ttttgcaacg acaacttttt gtccagcttc gatattgggt
     4501 gcaccgcaaa cgatttgcaa tactgtacca ttgtctactt ctgtctcagt aatcgataaa
     4561 tggtcagaat caggatgtgg gacgcatgtt tttacgtacc cgacaacgat cttcggttca
     4621 tgatcagctt caagctgttc ttcgaatcct gcttctttga tcaatcgatt caatttcgct
     4681 aattcctcat ctgttaagct tacttggccg ttcccttcga ttgttagatg atcggagata
     4741 tggaaaaagt tccaagcaac aacttgacct tgttcatctt tgatccgagc aacattccat
     4801 ttgcgttcaa cagtgttttc tgttccttta tcgtcggcta taataaccat caaggtgtct
     4861 ccgacgtgtg ctttgttata agcaaaaatc atgttctttc ctccattatc ctttgctgag
     4921 atttatctta acaaaaaatt gcattcactg catgaaattt ttttcatgct ttatttccga
     4981 agaagcctct tctggaacac catttattcg taacaaggga ctgtgacaag acttttgtca
     5041 cagtcccttt actgttttag ttaattgatt aaaaaggtcg tacaagttcc acttgcttaa
     5101 ttacttggct gttcttcttc gtcagaaacg atcaagacat cacaaggtga atgacgtatc
     5161 acgtagctgg ctacacttcc tgtcataaag cgttcgactg catttagacc tgactgccca
     5221 accatgatta gatcgatctc atattttttt ggcaatttga ctgccatgga ttcacgaaca
     5281 gaaccatatg caataattcc ttctatattt gtgtaatcaa cagatttccc atattcttta
     5341 cattcttcga tcatttcctt ggctgtttca gtttgctgat cgactagatt ttgatcaata
     5401 ggtgcatagc ccataaagtt ataaaattgc tgatcgatta cgtttgctac atatactttc
     5461 ccattattac ggcgagccac ttctaccgct tttttgaaag cagcaagtgc ttgttcacta
     5521 ccgtcgatac ctactaaaat attcttatat tcttgattca tcttgacccc tcctaacaga
     5581 tgtcagcctt ttgttacact ttctaggaac gattcgattt cctgttttgt tttacgatct
     5641 ttattcacta gacgacctaa ttcttttccg tcttggatca cgacaaaact tgggatacca
     5701 aaaatgttcc attcagcggc tacatctatg tacttgtcac ggtctacttc aataaattgc
     5761 caatcagaaa agtcattttc aatttcaggc atttgcggtt tgataaaacg gcagtctccg
     5821 caccagccgg cagtaaagaa aaatacactc tttccagttt ctacgtagcc tgctaattct
     5881 tctaaactat ttgggataat cattctttgt cctctacttt ctgtttatgt atttttcaat
     5941 taaattatac acctttcttt cgaactaaca aacattgatc ctctttttat aaaaaaaatc
     6001 tggcaaaaag caatcttttg ccagatttta tagtttcact atcgcattat tttccgtata
     6061 cgatcgtatt tactgttgat ttatccaaac ctttcagtgt ttgaatcaac atttctcgtg
     6121 cagcttcaaa atcgcgaata ctgaacatcg tttgatgcgt atggatataa cgtcccacta
     6181 cgccaatcac tgtacttggg attccttcgt tttgtgtatg agcagcacct gcatctgtac
     6241 cgccttttga aacaaagtat tgataaggga tattgtttgt ttcagcgata tccagcaagt
     6301 actcgcgcaa acgaggaagc atgatcaaac ctggatcttg gatacgcatc aatgtacctt
     6361 ctcctaaatg tccgaatgtg ccatttttcg tcactgtatc atctgctgca gagcagtcta
     6421 ctgcaaagaa cagatctggc ttgaatttat tgacagaagc tttagaacca cgcaagccta
     6481 cttcttcctg gacattcgcg cctgcaatca atgtatgacc taatgtttca ttttgcaacg
     6541 cttctaaagc ttctaatacc attgtacagc catagcggtt gtcccatgat ttactgataa
     6601 tattctttcc gttggctgtt ttgatcgttt cagcgtaagg aacgattgtg tctcctggaa
     6661 ggacaccgta actcattgcc tcttctcttg attcaaaccc agcatcgaac aaaatatcgg
     6721 ttacttctac tgatttttgt ccgcttgttc ctcgcaataa atgtggggga acggaagaag
     6781 aaatacatgg ataattctta ccagtagagg ttttaagtgt aaaacgctga gcagaaacaa
     6841 cgtaaggatt ccagccgcct agaggtacga ctttaaaaag cccgttttct tggatttgtg
     6901 tcagcatgaa tccgacttca tccatatgtg ctgcgaccat tacacggggt gcatccgctt
     6961 cttcatgatg gcgcagacca aaaatgccgc ccaaaccatc taactgtaac tcatctacca
     7021 gcggttccat atgactttcc atataagcgc gaacatcttg ttcaaaaccg cttgttcctt
     7081 gtaattctgt taactctttg attcgttgaa atgttttttc ttccatatgc tattcgtcta
     7141 aatttcagac taaactcctc tcagacttat attgatctgc ggataaaata tgattttttc
     7201 tgcagaccga tcgtatttat tatagcttat tttttctggt agcggttatt tatttcaaag
     7261 aaaatatgag agtgtggtaa aataagcaca aggagtaaca agtgagaaga aggaggtatg
     7321 tccacatgta tgaagaaagc gaactaagct attttaaagg cgggttagca gttggaatcg
     7381 gtctaggatt attgagcggg gtagcttcaa cgatttggta tcatcgcaaa aaagtattgt
     7441 cagcagacca agtacttgaa aatatcaagt catcttttct aaaagaaggt gcaatcgaag
     7501 gttcttggat ctcttttgaa aaggaaccta ttaggaaatt cgccgttcgg tcgagtgctt
     7561 atcggggagg tctttctcga atagaagatg gcgaaactgt ttattatgaa tttttagcag
     7621 atgctcatac aggaaccgta ttagaaattt caagaaaaaa aggtgcaaca gattaatacg
     7681 tcaaaaaagt gagagaacga atacgcaccc actattcaaa agagaggtat catgtccttc
     7741 ttttgtagtg ggtggttttt tagatagagc tttccaaaca tatattccag tattttcttt
     7801 ttaaataaat gatatttagc gggaaaaact agcatttcct ttgattatga ggtattttta
     7861 atttagcttt tgttttctat agttaaggag agtgaaaaat ggaacaacaa gaaaaaattg
     7921 ctgttctgat tccttgttac aatgaggaag caacaatttc aacagtcatt gcagacttta
     7981 aacgagaatt accagaagca gatatttatg tctatgataa taattcgacc gatcgaacat
     8041 acgaattggc cgttcaaggt ggagcaatcg taaaaaaaga acctcgtcaa ggaaaaggaa
     8101 atgtgatccg ccaaatgttc tttgatatcg atgccgacta ttatttaatg gtagatggcg
     8161 atgatactta tcctgcagaa gctgtacatg gtttgctcga gaaattacgt tctggtgaag
     8221 cagatatggt cattggtgat cgcctatcaa atggaaccta ctttgatgag aataagagac
     8281 ctttccacga ttttggaaat aaccttgtta gaaatacaat tactagaatg tataaaacaa
     8341 aaattcttga tgtcatgacc ggctatcgag gattcaatcg tatctttgtc aaaagttttc
     8401 ctattatgag ttctggtttt caaatcgaaa cggaattgac gatccatgct ttagataaaa
     8461 aattcaagtt cgttgaaatc ccaatcgatt atcgtgatcg tcctgaaggc agcgaatcaa
     8521 aattgaatac attctctgac ggattcaaag tgatcatgat gattattaaa atgtttaaag
     8581 attataagcc tttgcttttc tttgggattt ggaccatctt cttcttttta tttggattgg
     8641 tcacggggat tcctgtgatt cgcgaatata tgctgacaag ctttattaca cgcatccctt
     8701 ctgcgatttt aagtactgga ttgatgactt tagcacttct ttcattagta acagggttga
     8761 ttttggatac agtcgtcacg aacgctaaga aagaatatga attgaatcta tatcatgttt
     8821 atcatgaata ccacagtaga aagtaaaaaa aggatcaaga ctgcaaaaat aatttgcaat
     8881 tcttgatcct ctttttaatt aattatttcg ccaaacgata gatggcatct gcatagattg
     8941 ctgttgctct gaacaagtca tcaagactca taaattcgtt tgcttgatgc attgtatcag
     9001 tatatcctgg gaacatcgct ccataagcaa cgccacgttt caacaaacgt ccgtaagttc
     9061 cgccaccgat gatttgttcg tagccttttt ctccagtgtg gtcttcatag acttgcaata
     9121 atgtagccac caatggatca tccattggta cgtaatgtgg ttccattacg cgagcacctc
     9181 gagtaactgt tgcgccttct tgccccactg tttgttcgat gtcactttgc aatccgtcta
     9241 ctgttacacc ttttggaaaa cggaaattca tattgatgaa gttcgcttcc tctgtaccat
     9301 tttcttcaaa agcaaatagt ccagcattca ttgtcaattt gcccattttt tcatcttcat
     9361 aagcgactcc tagtttttct ccataaaaat cttcatggac ataagcagct gctacatgga
     9421 taaattgttt agcagatcct aggaattcat agttatctaa gaacaatgcc aagaaagaac
     9481 ctgcattgat accagattgt gggcttgcac catgtgcgcc tttaccaact aattcgattt
     9541 ttacatacgt attgtccgct tcaatcgttc cgcttactgg atttgcttcg atgaattggt
     9601 aaaatgcttc ttccatcgcg atagctgcat ctgcacttgg tacttgaagt gctgcagttg
     9661 ctgttcctgg caccatattt tcgcgaagtc cggaaacaaa agatttcaag acataatctc
     9721 ctgcattgtc gcctttgaag cgtaaagcta aagaaacatt tcctttttca ccattgatga
     9781 ttgggaattc agcatctggt gagaaaccaa aatctggttc ttcttcatgt tcgaaataat
     9841 aagccatatc gccccagccg ctttcttcat cgctgccgac tacgaagcga actttttttg
     9901 atagtttcaa gtcaagttct ttaatgatct tcatcgcata gtaagctgcc atacttggac
     9961 ctttgtcatc gcttgatccg cgtgcaaaga tctttccatc cttgattact ggttcataag
    10021 gatctgtgtc ccagccatct cctgcaggaa cgacatccat gtgtccaaat atccctagcg
    10081 tttcgtctcc ttcgccaaga tcaatatgcc ctgcatagtt atcgacattt tttactttaa
    10141 atccatcacg ttcgccataa gctaacatat gtttcaatgc gtcgcgaggt ccaggtccaa
    10201 atggtgcatc tggtgttact tttgagtcat cgcgctcgct gttgacacgt aaaagatttt
    10261 tcaagtcttc aagcaagtct tctttacgtg cttctacttc tttttgccaa tcaattgtca
    10321 taaagttaat cctccactta tttaagtctt cgtgttcatg ataccacaaa ttacagaaaa
    10381 aaatagttca aaaaatctga caattccttt tcttatctgt ttttctctaa aaaaaatata
    10441 cgctataatg agtaaaagga ggtttttaga tgctgataaa cgaacaacta gtgaaaagag
    10501 tcatcacacc tcgtccaaaa atttcacata aaggaacatt tggacgtatc gttttgattg
    10561 gcggcaatga acaatatggc ggagcaatca ttatgagtgc tttagctgcc gtacacagcg
    10621 gagcgggtct cacgactgta gtaactgatg aaaaaaatcg gagtgctctt cacagtcatc
    10681 tgccagaagc aatgattgta gtttggacgg atactgacca gtttctttcc gtaataaagc
    10741 aagcagatgt actcttgatt ggtcccggat tgggtacttc ttctaagagt ttggaacgtt
    10801 tcaaacaagt actagaaaac caatcaaaag aacaatggct ggtgattgat ggctctgctt
    10861 taagtttact tgctgctaat catcttttgc taccctatcc cgaacaaact gtacttacac
    10921 ctcatcaaat ggagtggcaa cggttgagtt ctctaccgat acaagaacaa cgggataaag
    10981 aaaacaaaaa gaagcaacag gaaatcggtg ctgtgctcgt tttgaaaagt catgagacaa
    11041 aaatttatgg tcaccagcac ttctacacca atcctttagg ttctgctgct atggcaacgg
    11101 gcggcatggg tgacacttta gctgggatga tcacaggatt tttagcccag tttcctcaaa
    11161 atgaccaaac gattgcagca gctatttatc ttcatagttt cattggagac cagctggcaa
    11221 aagaacaata tattgttttg ccgacacaaa tcagcgaagc acttccgaaa tggatgaatt
    11281 atttttgtaa aaaatcaatg gaaaacgagg catgagtcat ggagaaattt acaggttatg
    11341 tgcgagaacc tttttattat gaaacagatc aaatggggat tattcatcac agcaattata
    11401 tccgctggtt tgaagaggct cgggtagcct tattggtaca tctagggttt ccctataaaa
    11461 aaatcgaaga atctgggatc atcatccccg ttctggaagt aaattgcagc tataaagaaa
    11521 tgatccatta cggagaaaaa gtgagcatcc atccaactat ccaaaagtat acggggacac
    11581 ggttggattt tagctacgaa attgtgggaa ctgaagacaa aaaattaaaa actactggca
    11641 gtagtaaaca ctgtttttta ctttctgatt cgcaacgatt agtgaagctg agcaaagtca
    11701 atccggagtt agatcagtta ttccaagcgt acgcttcttt ggatgatgta aattcttaat
    11761 aaagaccgtc taagcatttt tcttgtattg tgtcgagtag ttcgtataat gaaaagaaaa
    11821 aagcaggtga tccccatgta tgtcgttaaa gtaatgcacg gttacatcga taaaactggc
    11881 tgtcgcactc gggaaaaaaa cttagacaat ctgctcattt tcaaagacaa aaaagaatcc
    11941 gaagcttttg ctaaaagaat cggcggtcgt gtcaaaccga tccaagaggt acgaccagat
    12001 tgattcaata tttttctttt cgaggctcta ataggcatgc ctatcagggc tttttttgca
    12061 gactgtgttt ttatgcccac acgatttgta agcgatttat gttataatag attatatctg
    12121 catagaaagg aaggttaaat ggttatcaat aatttgcaac agcaagctta tgaatctata
    12181 cgtaaacaaa tcatttattc cgagttagca cctggaagta aaatctccga taaagtgtta
    12241 gaagaacaac tagctatcgg gcgtacaccg attcaagaag cactgatcca actgcgcaat
    12301 caagagcttg taaaaaccat tccacagtcc ggcacttatg tttcaaaaat cgatatccga
    12361 tctgcttcat tagcccgtta tacgcgggaa aagttggaaa aaccgatcct gcaagaatgc
    12421 agtgcgaaaa tgaccaagca agatcaagct aaactagaaa aaatactaga acaaaccgat
    12481 caggcagtaa tcgctcaaaa taaaaaagct ttttttgatt tggatactga ttttcaccga
    12541 acttgttatg aaattgctgg aaaacgagaa atttgggatt ggctagaaag ttatagtacg
    12601 cacttgaacc gtttcagatg gttacgtttg acgatttcag aattagactg gggtcgagta
    12661 ttagatgaac atcagacaat gcttcaatca atgattgatc ataactttga tgaagtcgga
    12721 tttctttgca cgatgcattt acatatgatt atcgaggagc aagaatacgt gattcataat
    12781 taccccgatt attttgaaga tattcaggac agacccatca aataagaggt tttggaatca
    12841 gcgttttgac ctgagtatta gagaaagaaa cggcaaaata gttcctcata ttttgccgtt
    12901 ttttttgagc taataccgag ggtcagctga ttgcacaccg tttattgaga ggttgtgaaa
    12961 aagctgccct gcatcaagta ataagaacag ccaagtaaaa atagcttctt atgtttttaa
    13021 cttggctgga cttattaccg cagatgcaag cttttgaaca ccgttttcca agaggtcgtg
    13081 agcctgccgt tcatctccga gtcacaagga acaatttcaa aaaacagctt ctcatgtttg
    13141 ttgaatattt tgacttgtga ccgaggagtt gacaggagaa catcgtttat tcggaaaaaa
    13201 aggactgtga cacgactttt gtcacagtct ctttatttgt aatctaaatc atactccgta
    13261 catctttttt ctattttttt tatttcttct aagatccctt ggctacgaat ttcattatat
    13321 ggccaaagta agactgcctc taatacataa ggcgaaaaat cgctttgatt catctctttt
    13381 gaaaaactca tcgctgctat ctcgctcaat gcacgaatgt ttgattgatg tgtataaaaa
    13441 ggcaaccgcc acagcacaat tttttcgctt tgcgtataaa ttcccttaac agaagcttcg
    13501 atgtggtgaa atagttcatg tccaataatc aaagcttgcg ctgtttcttt agagatcgaa
    13561 gctagtcctg aacattttaa tttttctaac ggctccgtca tcaatcgaat cccttcatct
    13621 tctgcataag ttgcaaaagt gattcgttcg tttatttgct ctgattcaca ggaaacgatt
    13681 ggaacagcca tttgttcaca aatttctcct acatcggcaa aagggtactt ttttttgatg
    13741 ctacatgccg tttcgatacc acaatcgatt gattgttgta taagaatttt ctgcttggac
    13801 aaaggaatct ttttccaaaa taaatctcgc cgaaactggt agaatcccca aatttcttcg
    13861 gggatctgtt gtaattcgtt tagtaactct tctaacttat gtttgtttga tatttccggc
    13921 aacgacgatc acttcttcct ccggttccat agtagatact tccagatcca tcaaaagctg
    13981 tagttgttcc agatccgtcg ctgtaaaatc agagactctt gctcctaagc agagataaaa
    14041 ttctggtcgg gcaatcaatt cagtttgata aactgccata tcttcccaaa gttgttcgac
    14101 agctgtcata taattggctg cagttgtttt catacatgaa gcatgtccac gttggacctt
    14161 gataaatcct ttttgatcga tgatccgaag acttccagca ttttttgaac gattctgttc
    14221 gccgcataca taaaagaaag gtgtattttc caataagcaa acttcatttt tatttaatcg
    14281 catatcctct gcagcaagct ctagtgcttc ttctgttgtg atctccttgg ttagatcggt
    14341 tgctttcact tccgtagaac cagtagcgat agccgttact tttgaagtct gtggatcgat
    14401 ttctacgtgg acttcgatcg attctggtgt agcgcctgat tcaatcgctt tattcatcgc
    14461 ttcatttttc aacgatcgta tatcttcttt acttggcgaa gggatgatac gttctacaac
    14521 atctcggacc attgcaagtg ctacaccaat agaagaaatc acttcggcgt tttcaggaat
    14581 cgaatatttc acacccattt tattactaaa gtaagtaatc aatgaggcag cgccgccccc
    14641 tacacctaca agagaaatct gatctttctc caagtgatac ttgtctgcca attctaaaat
    14701 caccggttca atcttagcat aggatttttc catgatctgt tctgcgatgt cttcaaccgt
    14761 cgtatgacaa taatcagcta atgcttggat ggcttttcgt gcagatggga catttccata
    14821 agaaaaatgt tcttcttgca ctaatccaag gacattggct gcacatgtat ttgtaatagt
    14881 cacttcttct ccgtcttcca tcactacctt gacgtaatca gcagggtctc cttctttagg
    14941 agagaaaaat tcaaccttag gacctttgat tttttctgtc tccgtaaata ctgcataatc
    15001 tagaccggca atatgtgcag aacgtggacc gacatcgatg attcctgact gattagcccg
    15061 taccatcgaa ccgcctgcca cacctagaac ccgaacatct aatgaagaga tatatgttgg
    15121 gtggccacct acaattgaat aatcgattgc tggacgtcca tttttaatga ccccgatatt
    15181 ggttgttgtt ccgcctactt caaaataaac accattagac gctctcaagt acatcaaaga
    15241 gcccatgact gatgctgctg gaccggaaag catggttaaa acaggtcgtt ttttcatttc
    15301 attgatttcc attactccgc cgtctccgcg cataatcatc aaagaaacat tgacccctgc
    15361 ttctcgtacg gaatcttctg tagaggttgc cgtatctagc atttttggta aaatagatgc
    15421 attgattgct gctgtccgag ttcttcttgt caatccatat aattttgtga tgtcagaagc
    15481 catcgttgta gggatcgatt ttaccgatgc tgcttcataa acaactcgtt ctggttcccc
    15541 attatctact ccaaatgcca tagaagaaac caagacctcc gctctttctc tttctaaaga
    15601 agaaatcgtt tctgaaacac ggtctacatt taagtgtttg acaggtaaga atgcattgac
    15661 aatctctatt tttttcttat tccctaaatc gatatcgttc aatctagttt gtcgttttgc
    15721 taaaaaacct tctaatccac cttttgccat cccgattacg ccgacttttg ctacgtctcc
    15781 ttcaatcagt gcattagtcg cttgtgtggt agaatgtgca acaaacacaa catcttctgg
    15841 actgatatta ttttccagca aacaattttg aaaagactga acgacacctg cggctactcc
    15901 acggacatca tcgtgggttg ttttcactga agattttccg atgatttcgt gcgtcgcatt
    15961 atcgattgcg actgctttcg tatgtgtacc gccaacatca attcccattc gcacaaaacg
    16021 tttcattttc ttttcctcca ttcatctaat cgtttttatc gacgttttgt ttactaggcg
    16081 ttcccgtaca ataagaacgt gatcaggcat aagatcgctc cgatgatcca accagttggg
    16141 atggatttct tcatatattc tcgagaagtt acttttgtat aacctaatcc ccaagcaacc
    16201 caagattgtg taatatctaa atgctgagga gcaatcgttg tgattgcaaa taacgggaac
    16261 aaaaaggctg gcgacatcgt tggattaaca gctaatacga cagctaaaat agctgagcct
    16321 gaaccgacta gattcattgg accgcggaaa aatcctagtg gtgtcaatat agcaaatacg
    16381 attgccaaga ctaatggtga ttgcggtatc caatctccaa aaatagctga aaaataagga
    16441 gaagcatacg cagctgaatt attgaacatc gctaaggtca tcaagaaacc aaccattggc
    16501 gcaacatcta ctgcgccatc tgtaaataat ttcgcaaact gacgacagat ttctttataa
    16561 cctccgcgca attttccagt aaacagcaat gcccagatac ctgctagaat aaaacctaag
    16621 atgattggaa gatctaaaag aacgacccct aaaactggta ataatacggc taaccatgag
    16681 atcattggtg cgtcgccacc ttcagctggt acgtttgctg ccattgcata gcgtttcttt
    16741 ttgttcattg atatattagc gaccgttagt accacaacta agcttacaat catcccaatc
    16801 ataccaattt ggaaatagtc attataagta tagctttcag ccgctggatt gaaaccagca
    16861 taaatcgttt gatattgttt gaaattgacg atattagcaa agattccagc cataatcgag
    16921 cccataaatg agaacagtgc aaccggtact gaaataccta atgacatcaa gattggcaac
    16981 acaataactg caatagaaat cactggtccg attcctgtca tcgacatgaa tataacagcg
    17041 gttacaatac atagtaagct catcgtgatt cttggcttgt cccctccaag ttcaactacc
    17101 tttcgaatca gggtagctgc gattccgcta tctactagaa cacgcccgaa aaaggcacca
    17161 aagaagacat ttaccaagat cgatttggcg tactcagctg gccccgtcgt atagatatgc
    17221 gtcaacacat caattactcc ttgcccttcc atcgccgggt ttggtgaaaa cgtgtttccg
    17281 actagcgcta atcccatcca tacagtagcc atgatggcaa agccgaccat caagttgtag
    17341 ccttttacac agtaccagac catgaaaaag aatgagataa ctaacaaaat accaataata
    17401 atatcactca tgttattcct ccgtccattt tgtttttctg ataagcaaac taatatatca
    17461 gtcaaactca ttctaactta tgtagttagc gctttcatta aagggtgacc ataaatgttc
    17521 agttttcaat taaaaaacct taattatgct attttagact tatacgttat tgtcctgttt
    17581 catttgatgg aggattgtaa ataaaaaaag aggaaagaac ataacttttc gttatcttct
    17641 ttcctcttta taattgttta gcttatttag ccaaagcttc ttttgcttgg tcagccaatg
    17701 cagtaaatgc tgctgcatcg ttaacagcca agtcagctaa cattttacgg ttgatgtcga
    17761 tttcagcaac tttcaaaccg tgcattaatt ttgagtagct caagccattc atacgagctg
    17821 ctgcgttgat acgagcaatc cacaatttac ggaagtcgcg tttcttttga cgacgatcgc
    17881 gatatgcgta gttgtatgaa ttcattactt gttcttttgc tgttttaaat aaagtgtgtt
    17941 ttgaaccgta gtaacctttt gctaatttta gcactctttt acgacgttta cgagttactg
    18001 ttccaccttt aacacgtgcc atgtataatt cctcctaata aaaataaatc ttcagattct
    18061 gttatttgta cttcttattt catttttgct agttgttggc gaatacgttt gaagtcgcca
    18121 ctagaaacca tgcttgcttt acgcaattga cggcgttgtt tttttgtttt gccgtggaaa
    18181 cggtgacttg taaacgcgcg gaagcgtttc aatccgcctt taccagtacg tttgaaacgt
    18241 tttgctgatc cgcggtgtgt tttttgtttt ggcatgacta tatttcctcc tcaaaatctt
    18301 tcgttatccg actatcggct gtccaacagc cagttagtca ttcaaattac ttactgtcgt
    18361 ttttcggtgc cagcgtcaag aacatgctgc gtccgtccat tttcgctttt tgttccactg
    18421 ttgcaatatc cgcagtttct tcagctaagc gattcaagac tttttgacca atttctttat
    18481 gggtaatggc acggcctttg aaacggatag aagctttcac tttatcgccc ttttccaaga
    18541 acttacgtgc attacgaagt tttgtattga agtcattcaa gtcgatcgta ggacttaagc
    18601 gaacttcttt tacattgatc actttttgtt ttttacgcgc ttcgcggtct ttcttttgtt
    18661 gttcgaaacg gtatttaccg tagtccatga ttcgcgcaac tggtggtttc gcaccagggg
    18721 caactaacac aagatccaag tttgcttgtt ctgcaatatt caatgcttct gcttttgtct
    18781 taactcctaa ttgctcacca tctgaaccga tcaatcgtaa ctctcgtgca cgaatgccgt
    18841 cgttaaccat catatccttt gctatggtca ttcacctcca aatattatga gagaaagaaa
    18901 agcttaccga ataaacggca ataaaaaaac gagttctctt acagaacccg cgcgtacttc
    18961 acctaataga accagtgaaa actatctagc ccagtgacat tatcaactaa ggcgagaagc
    19021 gggtgcttct gcttttattt ctcaaccatc atagtatagc aaaaactaat ctttttgtca
    19081 atgtttttca tacagtttgt cttccttttc tgaaaatgat tagattagca gactttcttg
    19141 cagtgattcc cgcttctttg ctatactaaa gaaaaacgag gtgaacccac atgaaaaaaa
    19201 tcgaacctat tccaccttct cttcctcttc ttacaaaggg agagttttat ttagaagatg
    19261 aggagatctt ctctggtctt ctgatcaacg ggggtgacca aagctatctc gatgccaaaa
    19321 atatggtatt aagagagagt catatccaaa aacttacgat gcaaaaaaca gttttacgtc
    19381 attttgaatg cagtaatgtc atttttgaaa aatgtgattt ttccaatctg gagtggattg
    19441 gtggcagttt ccaccaagtc gtgtttcatc aatgtaaatt aacaggtacg aattttgccg
    19501 aaagttatct tcgagactgt actttcactg attgtattgc ttcgatggct tcctttagta
    19561 gtacaaactt gaaagctgtc tccttcgatc attgtcaatt agaagacagc gaattttatg
    19621 aggtcacttg gaaaaacctt tttttgtctg acaatcagtt gaccggttcg aactggtttc
    19681 gaacttctct gaagggatta gactttacga ctaatttctt cacagcaatc gctttatctc
    19741 aagattcttt ggcgggacta atcgtcaacc aagagcaagc acttgtgatc gcccaagccc
    19801 ttggtttgca gatcaaagac tagatcaacg aatccgccgc cacagtgcaa taagcatcca
    19861 aatagataag accaaactga tgatcaaggc gattccccat ccgaaaacgg aatgggtaaa
    19921 gggtaactgg agattcattc cgaagaatcc tgtcacaata gttggaacgg tcaagatcag
    19981 cgaccagacc gtcaaaaatt tcatcgtgtc attcaagttg ttattcagca aattattata
    20041 agtgccagag agctggtcta aaatctgcga agctaagttc gtcatttcaa ctgcttgtcg
    20101 tgcttcgatc agcgcatcgt ccaactcttc tttttccttt tctgacaact tgcgataaat
    20161 agccaatgcc ttgatttgtt ctaaaagtac tacattttgt tttgttcctg taactaagta
    20221 aaccaaaccg atctctaaat ctgaaagttg cagcagattc ttgttcgttg ttttttctct
    20281 taatttcaga ttaagccgct gacgttcact attcacttcc tcgattaaag gaaaaaagga
    20341 atcagaaatc aaaaacaatg tacgaaacaa taaactgtat acactcatgt ctggcattcg
    20401 ttccaaaagc gatcgaatta gtcgaatcac gtactcggta ttcggcgtgg taaatgtaaa
    20461 caaaccgtct tcttttaaaa taaaagcgat aggactagtt tcataatgat tatcgacttt
    20521 ttgctggtgc ggaacatttg atacaagcag taacgcttca tcaaaagaat cgtactctac
    20581 gcgcgcccgt tcatttttat ctagagaata agtcagcatc tcgtcactaa tgccatattc
    20641 ttcttttaag tttttaacag ctagcacatc gttttgctgt acattgatcc aaaaattacg
    20701 gtcttgatta aaatagtgtt tttcccgcat actctatcct cctgacattt gtttccagta
    20761 taaagaaaaa agtcgctcaa ataaaggttt accctttatt tgagcaactg taaatcaaat
    20821 ggctactttg gtttggcgaa tctctgctaa tttttcttcg atcattttca agacttcttc
    20881 ttttgcttga ggatcttcca caaaattata gcggtctcca tcgatttgga tttttggact
    20941 ttctttgtat tcttcatacc attgatcgta acgtctgttc aattctttgt aataatcgta
    21001 tagacttgga tcaaaggaca attgttcata cgaacgacca cgtttttcga tacgctccaa
    21061 catcgtatca aaagatacac gaatatgaac taataaatcc ggatgttttt tatgggcagc
    21121 atagggaagt tcttccatca tgttttccag cagttcatca tacacacgaa cttctgtttc
    21181 tgttgctctt cctagatctg catttagatg gaaaagcagt gaatcttcat aaatcgaacg
    21241 gtccaataca ttattgtcat ctttcattgc ttgtttgatg cttgcaaaac gtttattcaa
    21301 aaaataaatt tgcagcaaaa aagcatactg ttcagggttt gcataaaata agggcaagac
    21361 ttcattatca tcaatcgatt cataaaatgc ctgactatca aagtgttcag ccatcatttc
    21421 tgttaaactt gattttccgg caccaattgt acctgctaaa acgatcacgc tcatttacct
    21481 ccagctcact tcatcactaa atattgtgtt taactccaac ctttttaaat atacccccaa
    21541 tatctagtag tgtcaattgt atttttttct agtcaaagaa gaaaaaaagt caaaaaattc
    21601 ttaagaaaaa agtgtttttt ctttagaaaa cgtattctta gttttacttc tgagatggtt
    21661 ttagtaaaat aaaggaggaa tgttttttga gacccttagt ggacaaaaaa attcgtgaag
    21721 cagcgcaact cgttttgggc gctttatttt ttgcttaaaa atcactttta cgtgagaagg
    21781 agttagatta tggcaaacta tggccaagga gaatcaagcg gaaagatcat attgatgggc
    21841 gagcacgcgg ttgtttatgg agaaccagcg attgcctttc ctttctatgc aacaaaagtc
    21901 accgcattcc ttgaagagct ggatgcaatg gacgatcaac tggtttcttc ctactattca
    21961 ggaaatttag ccgaagctcc tcatgcatta aaaaatatca aaaaattatt cattcactta
    22021 aaaaaacagc atgacatcca aaaaaacttg caactgacca ttgaaagcac gattcctgct
    22081 gaacgtggaa tgggatcaag cgctgcagtc gccacagcag tcactcgtgc tttttatgat
    22141 tacttagcat ttcctttgtc tcgtgaaata ctattagaaa atgtccagct ttcggaaaaa
    22201 atcgcccatg gtaatcctag tggaatcgat gcagccgcta ctagcagctt gcagccgatt
    22261 tattttacaa aagggcatcc tttcgactac ttttctttga acatcgatgc ttttttgatt
    22321 gtcgctgata caggaatcaa aggacaaaca agagaagccg tcaaagatgt cgctcacctc
    22381 tttgaaaccc agcctcatga aactggacaa atgatccaaa aattaggata cttgacgaag
    22441 caagcaaaac aagcaatcat tgaaaattca ccagaaacgt tggcacagac aatggatgaa
    22501 tcacaatcac ttctggaaaa gctgacaatc agcaatgatt ttcttaatct attgatccaa
    22561 acagcaaaag ataccggggc attgggtgcc aaattaactg gcggtggacg cggtggctgt
    22621 atgattgcat tagcacaaac aaaaacaaaa gcccaagaaa tcagtcaagc acttgaagat
    22681 gcgggtgctg ctgaaacttg gatacaagga ttaggagtac atacctatgt ttaaaggcaa
    22741 agcacgcgca tataccaaca ttgctctaat caaatattgg ggtaagaaaa atgaagaact
    22801 catccttcca atgaacaaca gcctttcgtt aacattggat gcattttata cagagacaga
    22861 agtcatcttt tctgatagct atatggtaga tgaattttat ctagatggca ccttgcaaga
    22921 cgaaaaagca acaaaaaaag tcagtcagtt tcttgacctt tttcgtaaag aagccggcct
    22981 ctcactaaaa gcttcagtaa tcagtcagaa tttcgttcct accgcagctg gattagcctc
    23041 ctctgccagc gggctagctg ctttagcagg cgcttgcaat actgctctta agcttggatt
    23101 agatgatctc tctctttcaa gatttgctcg acgcgggtct ggttcagctt gccgaagtat
    23161 tttcggtggt ttcgtcgaat gggaaaaagg ccatgacgac ttaagttctt acgctaagcc
    23221 agtcccttcc gattctttcg aagacgattt agcaatggtt ttcgttttga tcaacgacca
    23281 gaaaaaagaa gtgtccagca gaaatgggat gcgtcggaca gtcgaaacat ccagttttta
    23341 tcaaggctgg ttagattccg ttgaagggga tctatatcaa ttgaaacaag caatcaaaac
    23401 aaaagatttc caacttctcg gagaaacgat ggaaagaaac ggactaaaaa tgcatggaac
    23461 gactttagct gcccagcctc cattcactta ctggtctcct gacagcttaa aagcgatgga
    23521 tgcggtgcga caattgagaa agcaaggcat tccatgctac tttacgatgg atgctggccc
    23581 taatgtcaaa gttttagtgg aaaacagcca tttatctgag gtacaagaaa catttaccaa
    23641 attatttagc aaagaacaag taattactgc acatgctggt ccaggaattg cgattattga
    23701 ataaagggag atttttatga ttgaagtatc tgcaccaggc aaactctata ttgccggaga
    23761 atatgcagtt gttgaaacag gccatccagc agttatcgct gcagtcgatc aattcgtgac
    23821 agtcactgta gaatctgcac gaaaagtcgg aagtatccaa tctgcacaat atagtgggat
    23881 gcctgtacgt tggacaagac gcaacggaga attagtttta gatattcggg aaaatccttt
    23941 tcattatatc cttgctgcta ttcgcttgac tgaaaagtat gcacaagaaa aaaacattct
    24001 tttatccttt tatgatctga aagtaacaag tgagttagac agttcaaatg gccggaaata
    24061 tggattgggt tctagcggtg ccgtgaccgt tgcaacggtc aaagccttga atgtttttta
    24121 cgcgttgaat ttatctcagt tggagatttt caagattgca gcactagcca atttagcagt
    24181 tcaagataat ggttcctgcg gcgacatcgc tgctagctgt tatggtggct ggatcgcttt
    24241 ctcaaccttc gaccatcctt ggctccaaga acaagaaact cagcattcta tcagtgagtt
    24301 acttgccctg gattggccag gtctatccat tgagccattg attgctcctg aagatttacg
    24361 tttattgatt ggttggacgg gtagccctgc ctctacttct gatttggtcg atcaagttca
    24421 ccgttcgaga gaagataaaa tggtggctta tcagcttttc ttaaaaaata gtacagaatg
    24481 tgtcaacgaa atgatcaaag ggtttaaaga aaataatgta acgttgatcc aacagatgat
    24541 tcgaaaaaac cgacaattac tgcatgattt atctgcaatc actggggtcg tcatcgaaac
    24601 gcctgctttg aacaaattgt gtaatttagc tgaacagtat gaaggagccg caaaatcttc
    24661 tggtgcaggt gggggcgatt gcggaatcgt aattgttgac cagaaatctg gcattcttcc
    24721 tttaatgagt gcatgggaaa aagcagaaat cactccactg ccgttacatg tctatagcga
    24781 tcaaagaaag gaaaaccgat gaatcgaaaa gacgaacatg tttcactagc taaagccttt
    24841 catgataaac aaaaaaacga atttgacttc gtacggatca tccataaccc tttgccgcaa
    24901 atagcagttt ctgatgtcga tctaagtaca caggcagttg ggtttacact aagcagccca
    24961 ttttatatca atgcgatgac tggcggaagc gaaaaaacaa aaaaaatcaa ccaagatctt
    25021 gcaatcgtcg caagagaagc agatttgatg attgccactg gttcagtcag cgctgccttg
    25081 aaagtccctt ctttagccga tacttatacg attatgcgac aagaatatcc tcacgggaaa
    25141 atcattgcca acatcggtgc agggacttct gtcgaaagag ccaaagaagc tatccgattg
    25201 tttcacgcag atgccttaca gattcatttg aatgcacctc aagagttggt tatgcctgag
    25261 ggagaccgtg attttaccaa ttggaaagta cttattcaag aaactcaaac agccattgac
    25321 gtccccctca tcgtaaaaga agtcggtttt ggcatgacaa gagaaacgct caatgatcta
    25381 gctgctttag gagttcatac agttgatatc agcggtcgaa gcggaacaag cttcacccag
    25441 atcgaaaacg cccgtcgctc caagcgggaa ctgagttatt tagctgactg ggggcaatcg
    25501 acagtctctt cattgcttga agcaaatgaa gcagacactt ctatggaaat cctagcctcc
    25561 ggaggtatcc gcaatgccta tgacatattt aaagcccttt gtctcggcgc aaatgccgtg
    25621 ggtacttcag gaaccgtact gacgcatctg atgaatcacg gcgtagaaga aactattata
    25681 ctgatgaagc aatggcaaga agagcttcgt ctgctttata ctatggtcgg cgcaacgaat
    25741 accgcggccc tacaccaaca atctctgatc ttttccggac cagtgaagga ttggtgcgaa
    25801 gcaagaggga tcgacttagt gaagtatgga agacgcacag aaaagagcgt gggacaaaag
    25861 taaagaacac ttttgtctca cgctctaaac acgtacaaac ggcggaagta aaagcaactc
    25921 cttcggaaat aagctaaaat tcacaaaaat ttgaaaagca attttcgtga attccttctt
    25981 atttcttgga gttaaatgct tctgtcccag cctctttttt cgaataaatg atgtttaatc
    26041 agctgtcctt cggtattcgt tcaatccgct gatttcacaa cctctccact aaacagtgta
    26101 ctaaagctct tttaaggttg ttttccgata taggcaagta tgcctccgtc cacatacaag
    26161 acatggccgt taacgaaatc agatgcgcta gaagccaaga agacagatgg tccagccaaa
    26221 tcgattggct cgccccagcg tgctgctggc gtacgtccaa cgataaattg atcgaatgga
    26281 tgacgttcac cgttttcttg tgtttcgcgt aatggagcag tttgcggtgt agcaatataa
    26341 cctggtccaa ttccattaca ttggatattg tattgaccgt attcagaagc gatgtttttc
    26401 gtcagcattt ttaatccgcc ttttgccgca gcataagcac tgaccgtttc gcgtcctaat
    26461 tcactcatca tggagcagat attgatgatt ttaccgccgc ctttttcgat catatccgga
    26521 ataactgctt ttgaaacgat gaatggggca tttaaatcca cgtcgattac ttggcggaag
    26581 tctgccgcat ccatttcgat catcggtgtc cgtttgatga tccctgcatt gttgactaga
    26641 atatcaactg aacctacatc ttctttgatc tgttgaacca tcgcttgaac cgcttcttca
    26701 tcggtcacat cgcagacata accttttgca tcgatgcctg cttctttata atgcgctata
    26761 ccttcatcaa ctgattcttg attcaagtta ttaaatacga tttttgctcc tgctgtagct
    26821 aaagagcgtg caatctcaaa tccaatacca tagacagcac cggtgactaa tgctactttt
    26881 ccatctaaac gaaacatatc catactaaat tccatcattt tctctccctt atgcttattt
    26941 taattgatcc atttgcacca tatccatgtc tgtataggtg atattttctc cgcacattgc
    27001 ccagatgaat gagtaattgc ttgtacctac acctgaatgg atcgaccagc ttggcgaaat
    27061 aatggcttgt tcattgtcta caactaaatg ttttgtttca tctggtttgc ccatcatgtg
    27121 gaacacacgt gtatcttcat tggcataatc aaagtagaca taagcttcca tacgacgttc
    27181 atgtgtatga caaggcatcg tattccaagc gcttcctggt tctaaaatcg tataacccat
    27241 ttgtaattga cagctttcgc atacatttgg atggatgtat tggtagattt tacgttcatt
    27301 cagtgtcagg ccttcaccag tctccattgg ttttacttgg tcgatactga tttttacatt
    27361 tggatattta tgatgagctg gaacagaact aatatagaat ttagctgggt tttcagcatc
    27421 ttgagaagaa aatacaacat gtcgtgtttc ttttccgata tagtatccgt cttgtttttt
    27481 catttcttct ttttgcccat cgatctcgat gaatcctggt ccaccgatat tgatcacacc
    27541 tagttcacga cgctctaaga agtaatcaac acctagttct ttgtctaatg tgatttctaa
    27601 ttctttttca gtaggtgtca ctccaccaaa gatcattcga tcgttgtgcg tataagtcaa
    27661 actgattttc cctggttcaa ataccttttc tactaaaaat tcttttctca attgttccgt
    27721 tgaataatga cggatatctt ctggactgtg tgtataacgt gtttccattt cttgcatttt
    27781 cttctactcc ttctatcgaa tgatttttcc agagccgcat gctaaaaatt gtgcgacttc
    27841 ttcttgacta aattggttgc agtccccatg gatcgtatgc ttcaaggcag aagctgccgt
    27901 agcaaaatca atgatttctt gcggagacat attggataaa atcccatgga gcacgccacc
    27961 tgcaaaagca tctccgccac ctactcgatc cacgattggg gtgattctat ggacttggga
    28021 ttccacatat tcatggttca tccacaatgt ccccttcaat tcattttcac ttgctgaatg
    28081 aacggtacgc ttggtcgaat aaaacacctg aatattcggg aacttttcct gcattttttg
    28141 ataataataa attagaggtt gttcttctgc ttctggcgct ttctcgatac ctaacaaata
    28201 aagagcatcc atttttccag ctgagcagat atctactaaa ggtaaaagct gatggattac
    28261 ttcgctggct tcttgctgtg tccataattt tccacgatag ttgatatcga agctgatcag
    28321 gcagccagct ttttttgcag cttctatcac ctgtttcgtc attttttgcc attttttaga
    28381 caaagctggg gtgattcctg aaacatgaaa tatatctatt ccttggaata actcctctag
    28441 cgaccattcc aaatgatcca tttcagcaaa acttgatccg gcacgatcat agatcactga
    28501 ggcggcgcgt tcgccactac cagtttccat ataataagta cctagtcttg atccaccaga
    28561 taatacctgt ttcgtatcta ctccataaca ttgcaagtgc tttttgactg cttttcccaa
    28621 tgcgttatca ggtacttttg tagcaaaaat cgcttcatgt ccgtaattag ctaaagaaat
    28681 tgctacgttt gcttcccctc cgccataatg ggcagcgaat cgttcacttg aatcgatacg
    28741 ggttccctgc tctgtggaca atcgcaacat gatttctccg agtgtcacta cttttcccat
    28801 aagcagctac ctcttgattt ctttcatttt tgctgcatac tgttgcgcga cttccgttac
    28861 tttgtcaaaa tctcctgtag ctgcgggtgc taaaaggttc ccacctactc cgactgttac
    28921 gacaccagca tcgaaccact ctttcatatt gtccagactc acaccgccag ttggcatgat
    28981 attgacttga ggcatcggcg ccttgaaggc agaaatgata cttggtccat aagcgcttcc
    29041 aggaaatagt ttgacgatat ctaccccgct tttcagcgct gttttgatct cggtaatagt
    29101 catacatcct ggtaagtaag gaatttgata cagattgcac atctctgccg tttcttgatc
    29161 aaaggaaggg ctaacaatat actctgcgcc agctaagata gccaagcgag ctgtgacagc
    29221 gtctaatact gtgcctgcgc caatcactat ttctggttgg tctttgtaga atgacaaaag
    29281 ttctttgatt acttgatctg cttgagggac tgtaaaagtc aactcaatac cagttagacc
    29341 acctttgatg attgcgtggc aagcattcaa agcttcttct ttgctttttc cgcgtaccac
    29401 cgcaatcact ccggcgtttt tcaatcgaga taaaatatca atttttttca tcttctccac
    29461 ctatttccta tttagaaatt ttattttata acttggaaat ctatacccat attatacggc
    29521 agaaaagaaa aattgcaaac gcttttttca ttatttttga attctttgct cgatttcgct
    29581 ttttgtttgc ttgagggcac tgattatttg ttcttttgtc tcatctgtta aacgatattt
    29641 cggcatactt acactaaatg caccaatgat ttgttcgttt ttcacgattg aggctcctat
    29701 acaaaaaata tcacgttcga tttcttcatc gtcaaatgcg atgtgggttt cttttaccgt
    29761 tttgatttcc tttttcaacc gtaatgcatt ggtgatcgta tattctgtgt aagggatcaa
    29821 cggatgcttt tcaagataat cagtgtactg ctcttcactg aattctgcta atacggcttt
    29881 gcccatcgca gaattgtata atggtcgggt gattccgatt tttgaagaca tgcggatcgt
    29941 ttgatgtttt ggatctagtt tgttcacgta caagatctca ttgttgtcca atacccctaa
    30001 gtggatagtc tcatcgatct tttgctgcag ctgttctaaa aacggtaaag tcaactctac
    30061 caaatcgatt tgttcaatgt ttttattcgc gtagcgaata agctttgcac ctaaacgata
    30121 gtttttctct gaatttttat ttacgtagcc gatcatcaac aaagtatcta gtattttcaa
    30181 gactgtagaa gaggtcattc ccacaccctt tgcgatcgtc tgcaaagaga catcttggtt
    30241 ttccgctaaa tagtctagta ttttagaggc tttgatcaat acagttccat aaggcttttt
    30301 attttccata taatattctc ctatttcttt ataagaaaaa ttatagcgga aataccatca
    30361 atttaaaaga gatatccaga tataaactac catttctctt tacaggcaat ttaatgttga
    30421 agatccgctt cgtttttaca tcaaactttg atataaagca atgatttctt ctactgtcgt
    30481 ttctcttgga tttcctggtg tgcatacatc tcgcaaagca tcttctgcaa ttgctggaat
    30541 atctgcttct tttacaccta attcagaaat ggtttctggg attccaaccg ctttgctcaa
    30601 ccgatcgatc tcaccacaag ccgcttctct tacatcttct aaagacattt cagctgctcc
    30661 tttaatccct agaacaagag cgatctcacg gtacttttct ccagtatatt ctttgttata
    30721 tttcatgaca gttggcaaaa gtgctgcaca cgctacgcca tgaggaatgt tgtaccatgc
    30781 acttaacggg tgagccatcc cgtgtaccag tcctaaaccg acatttgaga agcccattcc
    30841 cgcaatatac tgtcctaatg ccattttttc tcgcccattt tgatcgccat ctacagaagc
    30901 ttccaaagaa tggccgatga tttcgattgc ttttaaatgc agcatatcag tcatttccca
    30961 agcgcctttt gtgataaacc cttcaatagc atgcgtcata gcatccatac ctgttgatgc
    31021 agccaatttt ttcggcattc ccatcatcat atcgctgtcg atgaacgcga cgattgggat
    31081 atcatgagga tcaacacaaa caaattttcg gtgatttact tcatctgtga tgacgtagtt
    31141 gatcgttact tctgctgctg ttccagaagt cgttggtata gctaatatag gtaaacatgg
    31201 atttttcgta tcagcaacac cttctaaact aatgacgtct gaaaactctg gattcgtcaa
    31261 aataatccca atggcttttg ccgtatcgat tggcgaacct ccaccgattg cgatcaaaca
    31321 gtctgcctct gcctctttaa caaaagccac tccatctttt acattttgga tcgttggatt
    31381 aggtacgatc ccgtcgtata aagcataagc gattgctgct ttatctagaa gttctgttac
    31441 ttttgttgct ataccgtgct cgatcaatcc tttgtcagta atgacagctg ctttgccgaa
    31501 tcctcgctgc tgaaattcag ggacgatcga ctcaatcgct ccttttccaa aatacgatgt
    31561 ttcatttaaa atcattcgat tactcatttt ctattcctcc taatttgtat tatttttcac
    31621 aataaaaacg aaagaacccc aagcaaccgg ttgcccagtt gcttgttgct acagttcaaa
    31681 gactggcatc aattcccaac tgactggaga ggcatctgga tttgtctcca tgatatcttc
    31741 catgtacttc caccattttt gattgataga tgtatttgcc attttttccc ataacttttc
    31801 atcttcgatc actaagtaac caaataacaa acttgtactc ggatcaagat agatcgaata
    31861 gtcgatgacc ccgtgttcac gaagtgctgt acgcatttct ggccaaagtt catcatgtct
    31921 ttttttgtat tcttcttgtt tttctgggta aactttcatg caaaaagctt ttttcaccat
    31981 gtcagacacc tgctttcgta tgaacttcct ccaaataacc tgcttttgga gtgacaccaa
    32041 acgcatctgc taaatgccac aggttttcgt ccgtgattgt ttgcaagatt ggtccttgcg
    32101 ctttgacgta cgtatagact tctgctgcct tttctgctgt ctcgatcaaa ccaaagactt
    32161 catccatgtc ttttcctgac ccgtagattc catgttgtgg ccataaaacc aatcgagttt
    32221 ctttcatttt ttcagctgtt gcttccccaa tctcatttgt tcccggaacc atccacggaa
    32281 tgatggaaat tccttctgga aaaacaacta agcattccgt acacatttgc catagcgtac
    32341 gactaaagga acgttcgtct aattcatgcg taaaactcat cgctaataga tgactagcat
    32401 ggcaatgcat aacgatccga ttatctgggt ctacttctaa acgggcgata tgactcatga
    32461 aatgacttgg caactcgctt gtaggaaggc attcgttttc cagcccccac aaaagttcca
    32521 gtgttttgcc atcttcagca actcgaacga ttccaagatt ctcggcaggt tcgctcgcta
    32581 catttttaaa atatcttcct gaaccagtta caataaaata gcatcctgct aaacggctgg
    32641 catcaaaaat catcgagatg cggcggatca ctttttcagg atccaagaac ggtgtgatct
    32701 cttcttcttt tagtaaataa gaaatattcc cgccatttct ttcatcccat cccaagcgat
    32761 aaagattcga cgtcgtttcg atcatttctt tcacataagg cgcttgtaaa atatctattt
    32821 ttttcaatat agccacactc ctattttctt aattccaaca cgtctttttg atattcctgg
    32881 acttcttcca gccaatccaa accaactgga acttgatttt tgtaacagaa ataattccat
    32941 acatcggcat acgggaaatc tttcaattct tccgtataag ccattcttga agtaaagtca
    33001 aaagccagct ccatttcttt caatcgctca actggttcca gcatagcttt cagcagtgct
    33061 ttttgtgtat ttctcgtacc gatgacccaa gcagcgatac ggttgatcgt tgcatcaaag
    33121 aaatccagac cgatatttgt tttttccaac aattcgttac ggactaattc tttagcgatt
    33181 tcctgcaatt catcatccat caaaaccaca tggtctgaat cccaacgcac tggacgggaa
    33241 acatgcaaca tgattccttt gctgaataaa gctaatgaag ataatttatt ggaaatcact
    33301 tctgttggat gaaaatgtcc agcatctaga caaacaagtt tgtcacgagt caacccgtaa
    33361 cccatataaa attcatggga accgactgta taagcttcag cgcctaaacc aaatagtttg
    33421 ctttctactg catcttgtac ataccgttca tctaactctt ctgtaaaaat ttcatcgagt
    33481 gattccatta aacgttcccg tggggtaaaa cgatcaatcg gattatcttt catcccatca
    33541 ggtacccaaa agttattgat acttgtttgc ccagtttctt tcccaaagta ctccgcgatt
    33601 ttgcggcttc ttttcccgtg ttcgatccag tattcacgaa cttctttatc tggatgagcc
    33661 aatgtaaagc cgtctttcat cattggatgc gaaaagaaag tcggattgaa gtcaagaccg
    33721 atttcttctt tttttgccca ctctacccat ttttcaaaat gtttcggctc aatttcattt
    33781 agatcgacag attcttctgt atccaaatag atagcatgaa ggttcaattt gtgtttgccc
    33841 ggtatcaacg aataagcctt ctccagatct tgtctaagct cttcaggtgt tcgtgcggct
    33901 cctggaaaat tccctgtcgc catgattcca ccggtcaatt cgccatccgg tgtcaagaaa
    33961 cctttgacat catctccttg ccagcaatgc atagaaattt tgatgtcctg tatcttttct
    34021 agtacagcat ctgtatctac accgatagtt gcatattttt cttttgcttg ttcgtaattt
    34081 ctttcaatcg tgttcatacg attactcctt tgattttatt cacgtaccat tccatcgtgt
    34141 tttgcttatt ttcatctggg taaaaagttt cacatggaaa cgactgtttg attacatttc
    34201 ttgcttgttc tatattcgaa aactgacctg tactgagcat ttgcatggca acattcccga
    34261 ttgctgtggc ttcactaggt cctgcttcta caatgattcc ggcataatct gctgtcaatt
    34321 gattcagtag acgattattc gatccgcccc ctacgatgtg cagcttttcc aatttcgttc
    34381 cagttaagtg ttctactttg atcagctctt gtgcgtaaca aagagcaaga ttatcataca
    34441 cacaacgagc gatagctccc gatgagacag ggactggctg ctctgtttca aaacagtatt
    34501 gctgaatctc ttctatcata tttgggggat tcaaaaatcg agggtcattg atttccatca
    34561 cttgctgcag cggtttttct tttgcagcaa gctctgccaa ttccgcataa ctgtactcgt
    34621 actcttgcat tcgggcaact tcctggatca accacatccc catgatgttt ttcaaaaaac
    34681 gcaccgtgtt atgggcaccc cattcattcg tataattttc agaaaaagca ttgcgagaaa
    34741 cattggcgat ctttgtttca atccctaaaa gcgaccatgt cccactgctg atataacccc
    34801 aacgttctcc ttgacctggc gttcctaata cagctgacgc cgtatcatga cttgctactg
    34861 tgataaaggt cacatctggc aaatcatatt ttgaaaattg ttctttttgc agatctccta
    34921 gtatcgttcc cggctctgtc aatggtggaa ataattcctt cgagacaccg attttttcaa
    34981 gaagttctat ttcccattct tttgtccctg cattcagcag ctgcatagta gatgcattag
    35041 ttttctccgt caccatgtta ccagtaaaga cataccctaa ataatcaggt atcagtaaca
    35101 atttatgtgc ttttgccaaa ttttcttttt cttcagcaaa caattggaac aaggtattga
    35161 aaggctggat ctgaatccct gtctgctcat acaacttatc tagagaatag atttcagaaa
    35221 actgttctac tgctccattg gtacgcttgt cccgataagc aatcggatca tttatccgat
    35281 ccccttgttc atcaatcaga cagtaatcga ctgcccatgt atcgatcccc gcaaaaaggc
    35341 gatcaatccc tgtctgcttt aatttttcaa atccaatcaa aatctctttg ataagataat
    35401 cgatcttcca gcgatcgtga ccgtctttgt tccgaaagcc gtttttgaac cggtgtactt
    35461 cttccaagat caaagaacct tttgcatccc tcgtactttt cattagtcgt ccgcttgatg
    35521 caccgatatc gatggcaaca aatgttggat tcatttctcc acctctttta ctttattgtt
    35581 gattagcact ttattaataa agcgttatca tttattgaca atgctatcat aatacagaat
    35641 cttatatttt gcttcacact attttggtat ataattgcat tatcttgaca agttgggagt
    35701 gtttacatgg attactggga aaaagccaac cactcatggt cggaagactc aaaaagagtg
    35761 atcttgacgc ctacacaaag aagcaggaat ttattttttt atatccaaga aatcggttac
    35821 ttcaaagcac caatccctta ctatactgaa cgagcacatt taccttctta cttgataaag
    35881 tttactttgt ctggcaaagg ttcgcttaca tataatgata atcagcaaca gctaaaagct
    35941 ggagacttat tttttattga ctgccaagaa tatcagcgtt atcaaacgat cagcgaagaa
    36001 ccatgggaaa tggattggat ccatttgaat ggtcagaacg tccaatcatt ttatcaggag
    36061 ttcacaaaag acggccataa caccttccat tcaacaggag atccatcaga aaacaagatc
    36121 caccatttga tcacagaatt gatccacatg caacaaggca cgaatgcccg taccgatttc
    36181 cagtcctctg tagctatcca cgagttgctg aatgaactga ttttgcaaaa gtatcagcta
    36241 gatttcgaag aagaggatat tcccgcctac gtttacaaac tgaagcaata cttagacgaa
    36301 catttcaaag aaacgattac acttgatcaa ttagaacaac aattcttatt gaataaatat
    36361 cagctgagca aagatttttc caaatttatc ggttctccac caatcgatta tttgatcagc
    36421 aaaaaacttt cttacgctaa agaccttctt cggtatacag aatttacggt tcgaatgatt
    36481 tccgaagaaa tagggattga gaattttgct tactttagcc gcctgttcaa agcaaaaaca
    36541 ggactatcgc cgagagactt tcgtaaattc agctgattca tataatatgt acgtcatttt
    36601 ttcaaaatat tttgaaatat atagataaaa ataaaataac cgcagtttct gcttctatgt
    36661 aaacaacagc agctactgcg gttatttttt agtgatcatt ctcattgttc atcttcaaaa
    36721 tgttcataat gaaccaactg tttctgtgta tcacgtttca aaaattcttt aggcgttatg
    36781 ccgaactctc tcttgaatgc tttcagaaaa tgcggataag aagaaaaacc acttttacta
    36841 tatacagcac tagagctgcg gtattctcgc aacaagtgct tggcatagat cagctttttc
    36901 ttcattacat actgatgaaa cgtaaatcca gtgtactttt taaattctcg tgtgatatgg
    36961 tatttgctga caaaaaactg cttttccatt tcctctaaag aaagcggatg agataaattt
    37021 tttgagatat attgtaacat ttgttccaca cgctcattag gaactacagc attctgttct
    37081 tgcgcactct cttctttttg tacaacttga ttgagaaaaa tcaagtattc taccagagct
    37141 tgttcatatt ttatgtcttc accgtaaatt tttttatcag gaggatggtc gataaatgat
    37201 aatttttgct tcaatacttc ctgttctatt tgcaatacac gactcctctt ctctcccatt
    37261 ggttgaaaac aagccgatag attagaccat ggcgtggaac gactttctag aaaagaaggg
    37321 gtgacaaaga tatagacacg ctcgaataca tctgaagatt ggcggacgat acgatgcaaa
    37381 tcattggaat ggatgagcag cacacttcct gctttgatcg taaattgtcg tccgttcaaa
    37441 taaaaagtcg cttctccctt taatgtagca tggatctcat agaaatcatg atgatgatag
    37501 atcgttttat tttggctgac gacatctttg acatgataga tttcatactg atcagaaacc
    37561 atcgtattga cttgttcacc gctgattttc ttttggagca tgtgccgcac ctccttttgt
    37621 tcacttatgc acaattttat tataccacgt tttttgtaat caaaaagagc tgcttttata
    37681 tactcttata aaaaagaaga gacgtggcat cttctagccc cgccccctcc attctaaata
    37741 ttagttaacc aacgtcttct ttacattatt caagaagact tcttccataa cgaatcccat
    37801 catcttttgt ttcttcaaga accacataca ggtacggacg attcttcatg atgatttctt
    37861 tgatttttgt gtactcgatc acaccgtctc ccaaatttac cggcacgatt ttttcctgtt
    37921 cgacacgaaa atctttcaaa tgaaccgcac atatcttttc accaaaacgt tcaaaagctt
    37981 cttctaccaa ttggacttgt tcttgataat cactacttgt gatcaaattg gtcggatcta
    38041 aaatgattcc tagatagtcc gaatctactg cttggatcag ctgctctacc cttgctaaag
    38101 aatacaccgg atgattcaaa cctggttcta tccccaccat catttggtat ttttctccag
    38161 cttttaccaa gcgacggata acggacacag cttcagcaaa tgcttcatct gtgaaatttt
    38221 cttccgtata ttggatttcc ggcaaaacac atccagtttc cgaagcaacc atcgacgcac
    38281 caaaggaaga agcatagcga agatactgct cgaactttgt cagcaactct tgtcgtatcg
    38341 ttaaatctgg atgaatcata ttgatatagc aacttaaaat tcctacggtc acttcttgct
    38401 tttctaaaac tcgtttgaca taatttccca tcccagaatt gatctcattt gcgccagaag
    38461 gtatttcagg aaacgaaaga cctaaggcga gctgcagtgt atgaatccct tgttcttttg
    38521 ttttttctgc aagatcttct atcgtttgga cttcggaaag atcatgtcct ctaattgtaa
    38581 tgttcaacat aactattctc ctaactcagc atattgggta gccacatgac gatttgcggg
    38641 aagtaagtaa tcaaaaacaa gacgactaag ataaccccat aaaaaggaag aagcggtttc
    38701 agcattgcct ctgcttttac gcctcctaca cttgcaccga catatagtcc tgtacctact
    38761 ggcggcgtga tggagcctac acacagattc atgattgaaa acaacccgta atgtacagga
    38821 tcaaccccta cacttgtgac gattggtaaa aatattggtg taaaaatcaa gatagcaggc
    38881 gccacatcca taaacatacc caccagtaat aacacaagat tgactaataa cagaataata
    38941 aatttattat ccgttacgcc cataatcaac gaactaattg cctgaggaat accggtaaac
    39001 gccatggcga aagacatcat ggaagaagta gccaataaga acatgatcgt ccctgacatt
    39061 gctactgatt gaaacaacat attaggtata tcttttagtt ttaccgtttt atagtaaaac
    39121 atagcaatca acagggagta aatgacagct atcgctgaag cttctactgc tgtaaatatc
    39181 cccgtcaaga taccaccgat aatgatgaca atcaatagaa ggctaggaat cgcctctttt
    39241 attatttttc cagcatgagc catctgtcct ttttcgacaa cagggtattt attcttttta
    39301 gcaacgatga atgctactag catgattcct agcgcccaca aagcgcctac gagataacct
    39361 cccataaaca aagcagagat cgaagcacca ccagcaacaa gtgaatacat gataaaagca
    39421 gtacttggag gaatgaccat acctgtcgga gctgacgcga tattgaccgc tgtcgcaaac
    39481 ttccggtcat atccttcttc cacttgctga gggatcaaca ctccgccaat cgcggtagaa
    39541 gcggcaatgg ccgagctaga gatggaacca aataaagcat tccctaacac attcgtatga
    39601 gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa aatccactag tttcttagct
    39661 atccctccat tattcatgat aatgcccgag agaacgaaaa acggaatagc gatcagcgaa
    39721 aaactattga tacttcccac cattttttga gctgaactaa aagcagaaac atcaaacggt
    39781 ataactaaca ataaagtcat catcgatgag aatgcaatac ttatagcaat cggcatccct
    39841 aatacaagca acacaacaaa tacaaaaaat aacactaatc cggcttctat tgccatttta
    39901 ttcctccatt tttatatttt ttcttgaatt gctggcttgt tgcttaatgt atcgattaga
    39961 ttgatcaggc tatagataat gatgagtacc cccgatacag gtactgacag ataaacatat
    40021 cccataggaa tacttaaaga aggcgagatc tgccccattg tcaatgtgac agcattccat
    40081 ccgccataca ccatgatcat cgcagcaaat accagaaata aactttgaac aacaacttct
    40141 aaaattttac tttttttgcc agttaatttt tcacttaaaa ccgttacggc taaatgtgct
    40201 ttttgtccca caacatacgc ggaagacaac atggcaaacc agacaagacc gaatcgaaca
    40261 agctcttccg tgaccgtatt tggatttcct aaaaccgttc tggaaaatac ttgccataaa
    40321 acaacagcga ccatcccaac aagcaaaaca gctcccagga tctctaatac cttgtttaaa
    40381 atcattttca tcacagctac gctccttgct cttggatctt ttgataaagg tctttgtagg
    40441 taggatcgtt tttgaatata tcatgaagtg gggcaactgc ctctcgaaac ggtgtattat
    40501 ctacatcatt gaattggact ccatgttctt cttgtgctaa agctttttct tcttcaattg
    40561 cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt cgctgcttct tcaatggctt
    40621 tttcctgctc atcagttagc tgttctttga tggcatcatt catgatgatg atatctggca
    40681 cacgtgtatg ctcatcatag gaatagtatt tagccacacc accatgtcca gctgtataca
    40741 agacaaattc attgttttct gatccgttga tcaaattgga ttgaagagac gtgtacactt
    40801 catcacttcc cataggtacg ggcgaaccgc ctaaaagctc taccattttg atagcggttt
    40861 cgctttgcat cacacggatc ttctttccct tcaaatcttc cggcgtatgg atcggaccgt
    40921 ctaccatata aaagctgcgc tgaccagaat cataataggt caatccggta aatcctaaat
    40981 ttttggtcga ttggtagatt ggctccatta tttcttcatt atccatcacc ttaaagaaat
    41041 gttcttcatc attgaataga taaggaactg aaaaaatcga ataatcttta gagaaacttt
    41101 ccaacgctga ggcacttact tttgtgaaat ctactgctcc tgactgtgtc aattcgatca
    41161 gttcacgttc tccacccagt tgtccatctg ggaaatactg gatctgtacg gaaccatctg
    41221 ttttttcttt tactaaacgc ccaaattcat tcattgcatc aattactggc tggctgttgc
    41281 ttgcataagc aaagcgcaag gtaactatct catttacatc tgcatctgcg acagttgcca
    41341 cactagaatt atttccgcag ccgcttagac ctagcagacc taggctaagc gaacttgctg
    41401 caaacagcag tgatgttttt ttcatatggg tttcctttct tttaacaata agagacgagc
    41461 aattctgtca aacaaagcac cgttaacgac tgtccataag gcattgctgt gattccgata
    41521 ttccgataaa aatctaaact gtcccccatt cctgtcccga ttgaaacgtt ttgaacttct
    41581 cctttttcat cgatttgttc aagcaatcct tggatagcac gataagcgac ttctttatat
    41641 tcttgtggta aataacgttt atggaccgct ttcaaaatac cgtaagcaaa tcctgcagtt
    41701 gcagaagatt ctacgtaaga agaaggatca tccaacaacg tatgccataa accgctttca
    41761 gactgataag tttttaatgc acgcacttgt gcttcaagtg tttcgatcaa taatttgcgc
    41821 aatgcatctt ctttcgataa ttctaagatt tcaatgatct ctggaatagc aatcgtcaac
    41881 cagcagtttc cacgagccca aaatgcttcg gcatagttat gatttccttc aaacgtccag
    41941 ccgtgatacc acaatcctgt tcgtttatcc atcaagtatt tgatatggat cataaactgg
    42001 taacgagctt cttctaaata ttctgggcga ttcaacagtt tcccgatttt tgccaaagga
    42061 agcactgtca tcatcaatgt atcgtcccac aactgattct tattttccgg tccatatgtg
    42121 gcatgctgta acccgtcttc atttgtccga ggcatgtcat tcatgaccca ttctgcccat
    42181 tgttctaaat aaggcaaata gcgtgaatct ttcgtgtctt catacaagta agccatagtc
    42241 aatagcggtg ccattgtatt gacgtttttg ggcggagcgc cttcttccat acgatcctca
    42301 aaccattcat tgaccacttg ataagcgcga ggattttttg tcatctggta atttttataa
    42361 ataccatata gaccgacacc ttgtggccaa ttccacactg tccagctttt gtcatctact
    42421 ttcagtccat caaagtccaa caaaaattca ccattatcgt ctttgatcgt tgtcaggttt
    42481 tcaacgagca ggtcaatttt ttctttcact aattctttag gtacaaaaat ctcttcttta
    42541 ataatcatct ttacgccacc tttttaaacg ctttcatttt atatccttaa tatagcagta
    42601 ataccgtcca ctataaatat cgctttttga acaaaaactg tctaaaattg acctaaatgt
    42661 taaataacaa aagtaacttt cagttacttg gaaattaaaa aaagaacttg gagaaacaac
    42721 gttttccaag ttctttttca tctattttct tatctttttt ctacgctttc ttttgcttat
    42781 ccgttgtaag catcaatgat gatctgtttc aattcagaga tcaatggttc tttcggatta
    42841 gctgtcgtac attggtcttc gtaagctaat tcagccatac ggtctacggt tgtatctagt
    42901 gtttcttgtg tgactccttg agcttttaga ctcatgttga tgccgacaga ttttcctaat
    42961 tcataaacag ccgttgctaa agcttcgact aattcctctg ttgtttcgcc tttcagacct
    43021 aagaattttg cgatatctgc gtaatctgta tctgcacgga aatagtcata tttagggaac
    43081 atcgcatgtt tttgtggatc ttttgcatta tagcggataa tatgcggtaa aagaatcgca
    43141 tttgtccgtc catgagggat cccgtattcc ccgccgattt tatgtgcgac tgagtgacaa
    43201 atacctagga atgcgttagc aaacgccata ccagccattg tagaagcgtt gtgcattttt
    43261 tcacgtgatt ccatatcagg tgtttttact gatttttcta aatagtcaaa gactaacttg
    43321 attgcttgta ggctcaatcc acgtgtataa tctgaagcca tgactgaaac ataggattcg
    43381 attgcatgtg ttaacacatc catcccggta tctgctgtga cagatgctgg aactgacatg
    43441 acgaattgag gatcgacgat tgcgacatct ggcgtcaatg cataatctgc taacggatat
    43501 ttcacatgtg tttcactgtc agtgatgacc gcaaatggcg ttacttcaga accagtccct
    43561 gatgttgtcg ggatacatac gaattgtgtt ttttctggtt tgtcgatctt atatgtccgt
    43621 ttacggatat ccaagaattt ttgttttgca ccaaagaaag atgtgtctgg gtgttcatag
    43681 aacatccaca ttcccttagc tgcgtccatt gcagaaccgc ctccaagagc gattactgta
    43741 tctggcttga agtctgccat cagtttcgtt cctgcgtata cggtatttgt cgatgggttc
    43801 ggttcaacat ctgagaagac ttcgatcttc acatcatttt tacgtcgtgc caatacttca
    43861 cgaactgtat cgcagtaacc gaattggacc ataccagggt cacaaacgat catgacacgt
    43921 tcaacgtttt ccattttctc taagtacaat agagagttct tttcaaagaa gatttttggc
    43981 ggtaatttga accattgcat gttatttctc cgtttcgcta cagttttgac attgattaag
    44041 tttacagcag agacgttacg agaaacagag ttcttaccat aagaaccgca tcccaaagtc
    44101 aatgacggaa tcatttcgtt atagatatca ccgatgccgc cttctgcaga tggtgtattc
    44161 actaaaatac gacaagcttt cattcgaaga ccgaatttga catgcaactc ttcgttttct
    44221 gaatggataa ctgctgtatg tcctaatcct ccaagattca acataccttc acacaaatca
    44281 aacgcatggt ccgtgttgtt agctttgatc atggcaagaa ctggagaaag tttttctctt
    44341 gatagcggat aatctgggcc tacgccttcc aattcagcaa tcagcatttt cgttcctttt
    44401 ggtacactga ttcctgccaa ttttgcgatt tccatcgcag aatgtccaac gattgatgga
    44461 ttgaccgcat attttccttc gttcatcact gcatcttcta atttttgcaa ttcatctggt
    44521 tttacgatat aaacttggtg tgcttggaat tctgctttta ctgctgcata gatttcttta
    44581 tccacgatca cagcttgttc agaagcacaa atcattccat tatcaaatgt tttcgaaacg
    44641 atcaaatcgt tcactgcacg tttaattttt gctgtttttt caatataagc tggtacgtta
    44701 cctggaccta cacctaaagc tggttttcct gtagagtaag cagacttgac cattccagct
    44761 ccacctgtcg ctaagacgat cgcgatacct ggatggttca ttagcataga agtcgcttcg
    44821 atcgaaggat gttcgatcca ttggatacaa ttttcaggag ctccggcagc gatagcagca
    44881 tcacgtacca ctcgtgctgc ttctgcagaa gatttttggg cacttggatg gaaagcaaaa
    44941 acgattggat ttcttgtttt caacgcgatc attgatttga aaatcgttgt ggaagtcgga
    45001 ttcgttgttg gagttactcc gcaaaccaca ccgactggtt cagcaatttc aatcaaccct
    45061 ttttggacat cttcattgat cacaccaact gttttatcat gtttgatatt atgccaaata
    45121 tattcggacg catacatatt tttgattgct ttatcttcgt aaacaccgcg gcctgtttcc
    45181 tctacagcca tcttcgctaa tggcatatgt tgatccaaag ctgccattgc catttcatgc
    45241 acgatatggt ctactttttc ttgatcaaag ctttccattt ctttcaatgc tgcatttgct
    45301 ttttctgcca aatcatcaat cattgcttgt acgtcaattg tcttcgtttt ttctttttct
    45361 attactttag ccatcattat cctcctaatg ggtcatggtt atttgttaaa taattcacat
    45421 gtatatcata caatatttat ttttctatgt aaacactttt ttgtgatttt tttcacagtt
    45481 atttttaaat ataaaattgc tttatttaca ggtgtttgta tag
//
LOCUS       DAZKOW010000011        40500 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000011 DAZKOW010000000
VERSION     DAZKOW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.011"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..330)
                     /locus_tag="ACG912_000622"
     CDS             complement(1..330)
                     /locus_tag="ACG912_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFQ0511967.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            507..1235
                     /gene="sfsA"
                     /locus_tag="ACG912_000623"
     CDS             507..1235
                     /gene="sfsA"
                     /locus_tag="ACG912_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFQ0511968.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            1251..2264
                     /locus_tag="ACG912_000624"
     CDS             1251..2264
                     /locus_tag="ACG912_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0511969.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            2264..3541
                     /locus_tag="ACG912_000625"
     CDS             2264..3541
                     /locus_tag="ACG912_000625"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0511970.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            3604..6306
                     /locus_tag="ACG912_000626"
     CDS             3604..6306
                     /locus_tag="ACG912_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0511971.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYDQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            6458..6775
                     /locus_tag="ACG912_000627"
     CDS             6458..6775
                     /locus_tag="ACG912_000627"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0511972.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            6805..7125
                     /locus_tag="ACG912_000628"
     CDS             6805..7125
                     /locus_tag="ACG912_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0511973.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            7233..8693
                     /locus_tag="ACG912_000629"
     CDS             7233..8693
                     /locus_tag="ACG912_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0511974.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            8761..8982
                     /locus_tag="ACG912_000630"
     CDS             8761..8982
                     /locus_tag="ACG912_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511975.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            9013..9195
                     /locus_tag="ACG912_000631"
     CDS             9013..9195
                     /locus_tag="ACG912_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFQ0511976.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            9195..9608
                     /locus_tag="ACG912_000632"
     CDS             9195..9608
                     /locus_tag="ACG912_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFQ0511977.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            9731..10912
                     /locus_tag="ACG912_000633"
     CDS             9731..10912
                     /locus_tag="ACG912_000633"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0511978.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(10983..11147)
                     /locus_tag="ACG912_000634"
     CDS             complement(10983..11147)
                     /locus_tag="ACG912_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511979.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            11301..11374
                     /locus_tag="ACG912_000635"
     tRNA            11301..11374
                     /locus_tag="ACG912_000635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11335..11337,aa:Arg,seq:cct)
     gene            complement(11443..12582)
                     /locus_tag="ACG912_000636"
     CDS             complement(11443..12582)
                     /locus_tag="ACG912_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0511980.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAGLLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            12754..12888
                     /locus_tag="ACG912_000637"
     CDS             12754..12888
                     /locus_tag="ACG912_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511981.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(12881..13516)
                     /locus_tag="ACG912_000638"
     CDS             complement(12881..13516)
                     /locus_tag="ACG912_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511982.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(13629..14264)
                     /locus_tag="ACG912_000639"
     CDS             complement(13629..14264)
                     /locus_tag="ACG912_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFQ0511983.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(14298..14759)
                     /locus_tag="ACG912_000640"
     CDS             complement(14298..14759)
                     /locus_tag="ACG912_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HFQ0511984.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(14889..15320)
                     /locus_tag="ACG912_000641"
     CDS             complement(14889..15320)
                     /locus_tag="ACG912_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0511985.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(15338..15658)
                     /locus_tag="ACG912_000642"
     CDS             complement(15338..15658)
                     /locus_tag="ACG912_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0511986.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            15957..16733
                     /locus_tag="ACG912_000643"
     CDS             15957..16733
                     /locus_tag="ACG912_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFQ0511987.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            16748..16951
                     /locus_tag="ACG912_000644"
     CDS             16748..16951
                     /locus_tag="ACG912_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0511988.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            16967..17305
                     /locus_tag="ACG912_000645"
     CDS             16967..17305
                     /locus_tag="ACG912_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFQ0511989.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            17292..17471
                     /locus_tag="ACG912_000646"
     CDS             17292..17471
                     /locus_tag="ACG912_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511990.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17514..17984)
                     /locus_tag="ACG912_000647"
     CDS             complement(17514..17984)
                     /locus_tag="ACG912_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511991.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            18071..18769
                     /locus_tag="ACG912_000648"
     CDS             18071..18769
                     /locus_tag="ACG912_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFQ0511992.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18947..19288
                     /locus_tag="ACG912_000649"
     CDS             18947..19288
                     /locus_tag="ACG912_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511993.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19281..19952
                     /locus_tag="ACG912_000650"
     CDS             19281..19952
                     /locus_tag="ACG912_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFQ0511994.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            19958..20650
                     /locus_tag="ACG912_000651"
     CDS             19958..20650
                     /locus_tag="ACG912_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFQ0511995.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            20647..21396
                     /locus_tag="ACG912_000652"
     CDS             20647..21396
                     /locus_tag="ACG912_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0511996.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            21408..21677
                     /locus_tag="ACG912_000653"
     CDS             21408..21677
                     /locus_tag="ACG912_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511997.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            21683..21847
                     /locus_tag="ACG912_000654"
     CDS             21683..21847
                     /locus_tag="ACG912_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511998.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            21840..22139
                     /locus_tag="ACG912_000655"
     CDS             21840..22139
                     /locus_tag="ACG912_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0511999.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            22139..22441
                     /locus_tag="ACG912_000656"
     CDS             22139..22441
                     /locus_tag="ACG912_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0512000.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            22481..22846
                     /locus_tag="ACG912_000657"
     CDS             22481..22846
                     /locus_tag="ACG912_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512001.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            23336..23476
                     /locus_tag="ACG912_000658"
     CDS             23336..23476
                     /locus_tag="ACG912_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512002.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            23473..23655
                     /locus_tag="ACG912_000659"
     CDS             23473..23655
                     /locus_tag="ACG912_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512003.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            23668..23862
                     /locus_tag="ACG912_000660"
     CDS             23668..23862
                     /locus_tag="ACG912_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512004.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(23895..24107)
                     /locus_tag="ACG912_000661"
     CDS             complement(23895..24107)
                     /locus_tag="ACG912_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512005.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            24235..24435
                     /locus_tag="ACG912_000662"
     CDS             24235..24435
                     /locus_tag="ACG912_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512006.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            24432..24704
                     /locus_tag="ACG912_000663"
     CDS             24432..24704
                     /locus_tag="ACG912_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFQ0512007.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            24705..24890
                     /locus_tag="ACG912_000664"
     CDS             24705..24890
                     /locus_tag="ACG912_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512008.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            24868..25224
                     /locus_tag="ACG912_000665"
     CDS             24868..25224
                     /locus_tag="ACG912_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512009.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            25184..25660
                     /locus_tag="ACG912_000666"
     CDS             25184..25660
                     /locus_tag="ACG912_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0512010.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(25806..26534)
                     /locus_tag="ACG912_000667"
     CDS             complement(25806..26534)
                     /locus_tag="ACG912_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFQ0512011.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            26582..26860
                     /locus_tag="ACG912_000668"
     CDS             26582..26860
                     /locus_tag="ACG912_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512012.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            26862..27248
                     /locus_tag="ACG912_000669"
     CDS             26862..27248
                     /locus_tag="ACG912_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0512013.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            27245..27625
                     /locus_tag="ACG912_000670"
     CDS             27245..27625
                     /locus_tag="ACG912_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFQ0512014.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            27737..28189
                     /locus_tag="ACG912_000671"
     CDS             27737..28189
                     /locus_tag="ACG912_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFQ0512015.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            28186..29913
                     /locus_tag="ACG912_000672"
     CDS             28186..29913
                     /locus_tag="ACG912_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFQ0512016.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            29977..31164
                     /locus_tag="ACG912_000673"
     CDS             29977..31164
                     /locus_tag="ACG912_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0512017.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            31136..31705
                     /locus_tag="ACG912_000674"
     CDS             31136..31705
                     /locus_tag="ACG912_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFQ0512018.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            31718..33082
                     /locus_tag="ACG912_000675"
     CDS             31718..33082
                     /locus_tag="ACG912_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0512019.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            33084..33365
                     /locus_tag="ACG912_000676"
     CDS             33084..33365
                     /locus_tag="ACG912_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0512020.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            33343..33669
                     /locus_tag="ACG912_000677"
     CDS             33343..33669
                     /locus_tag="ACG912_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0512021.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            33659..33997
                     /locus_tag="ACG912_000678"
     CDS             33659..33997
                     /locus_tag="ACG912_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512022.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            33987..34352
                     /locus_tag="ACG912_000679"
     CDS             33987..34352
                     /locus_tag="ACG912_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0512023.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            34359..34985
                     /locus_tag="ACG912_000680"
     CDS             34359..34985
                     /locus_tag="ACG912_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0512024.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            34985..35449
                     /locus_tag="ACG912_000681"
     CDS             34985..35449
                     /locus_tag="ACG912_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512025.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            35644..37953
                     /locus_tag="ACG912_000682"
     CDS             35644..37953
                     /locus_tag="ACG912_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0512026.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            37965..38669
                     /locus_tag="ACG912_000683"
     CDS             37965..38669
                     /locus_tag="ACG912_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0512027.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            38666..>40500
                     /locus_tag="ACG912_000684"
     CDS             38666..>40500
                     /locus_tag="ACG912_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0512028.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        13913 a         6480 c         8215 g        11892 t
ORIGIN      
        1 ctatttttca gataacaact tatatgaagt gtctgcagat tcgtcataaa gagtttgttc
       61 gggataagaa aagataagat aaaaaccata ctgatcacgt acgactaaac gatattcatc
      121 tatttctacc acagatgtgc caagagactg accgttgatc aaaaacgtgt aatcagatgt
      181 aatctgtaat tgttgttttt gctggctatc actaaagaac catgtacctt gatactttga
      241 gagtccttct gacttttccg cttgtttttt ctttttcaag taaataaaac ttgagagaac
      301 ggtaatgagt gaaccaagaa aatagatcat tttttttatc accaaatcac tcctttatga
      361 gaaaataatc gcatagacat attctttctt ctttatttca ttgtaccgaa taaaagctaa
      421 aatttctcta agactttcga ctggtttttt tactgttttt ctacaaatta tgataggata
      481 catagtagga agaaaggtga cataatttga agtatcagca agtggaaata gcttttttta
      541 ttgaccgccc gaatcggttt atagcacatt gtatgaaaaa agacggagaa ataattattg
      601 cgcatgtcaa aaatacaggt agaggaaaag aggtcctgat accaggcgct gaagtggcta
      661 ttgtatttgc tcctggacct aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga
      721 agggtgactg gtttaatatc gatagtcaat tgcctaatcg gctggcaatt gatggtattt
      781 tagacggcac gatcctttta ccaggattaa ctggtccatt tacggtatac aagcgtgaag
      841 taacttatct tcactccaaa tttgatattt atttagagac ggtggtcgga caaaaagcat
      901 ttgttgaagt caaaggtatg acacttgaaa ataaagcgat tggtgctttt cctgatgcgc
      961 ccacactcag aggattgaaa catataggag aattaacgta tgccgcacaa gatggttatg
     1021 ccgcttatgt cttgtttatt gctcagttcg aacatcttca tctggcaacg atccatgaag
     1081 aaatgcaacc agcactagct gatatggttc gacatgcgca gcaaagcgga gttcaaatcc
     1141 tcgcctataa ttgtcaagta tcggcagatg aggtaactgt caagcaggcc attccatttg
     1201 atgtagatat cccttttgaa gatcctatac aatagaaaga aggtaaaata atgatcaact
     1261 taggtattat cggaacaaat tggattactc atcaatttgt ccaagcagct ttaagcacaa
     1321 aaaaatatga attgacagcg gtatattcta gacatttgga aaaagcacag gaatttggtt
     1381 ctcattatga aggggatatc gaattcgcaa cagatttgaa gacttttttc aatattactc
     1441 atatggacac catttatatt gcttctccga atagtcttca ctttagtcaa gctaagcaag
     1501 caattttggc aggaaaaaat gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa
     1561 tggatgaaat catccatttg gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa
     1621 atatccatga atcaggtttt aaaaagattg ccgattactt accattaaaa gacgagataa
     1681 tcggtgcaaa tttcagctat atgaaatatt cttcgcgcta tgaccaagta ttagatggag
     1741 aagaaccgaa tatcttctcg actcatttct caggtggtgc attgatggat ttaggtgttt
     1801 accttgtata tgcagctgtt gcttggtttg gaatgccaaa agaagtccat tatttcccaa
     1861 gaaagatttc tactggtgta gatggtcttg gatgggggat cttacgttat gagaattttg
     1921 atgtatcgat ccagccaggt aaaatcggtg attcttattt accgtcagag atttattttg
     1981 actcaggaac gctagtgatg aatggagtca atgcaattga acaagcacaa tttcatgatc
     2041 gcgcgcataa agaaatcatt gatttggcgg taacagctgc agaaaatcca atggcagatg
     2101 aagcagagga ttttgccaat gtcatggctc atccgaaaga tccagcttgg ggattgttgt
     2161 atgaagagtg gatcgagcta gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa
     2221 atggcggcat caaattcgat gcagataatg aggaagattt ttaatgaacg aatttgtaca
     2281 agtatttccc gaaaattggc agaaaaaata tgaggataaa gggtttatct caccttctct
     2341 tattcaacga gcagtttttc agccactaag taataaacaa agtatagtag cggtttctcc
     2401 tacaggttca ggtaaaacac tggcttatct ttggcctcta ctactaaacg tggaaccagg
     2461 tgaagcaagt gcgttggtca tttttgcttc ttctcaagaa ctagctattc aagtagcaga
     2521 tgttgcacgt gaatggggaa aagacaaaga gttgaaagtc caatctttgg tgggcggagc
     2581 caacgtcaaa cgtcagattg agggtttgaa ggaaaaacca gaaatcttga ttggtacacc
     2641 aggaagaata ttggaattga tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat
     2701 ggtatttgat gaagcagacc agctatttga cgctgggaac agccaaatca ttgatcaaat
     2761 tcttcatcaa gctccaacag agtatcagct agcttttttt tcagcgacag cagatcgttc
     2821 ccttgaaagc attgaaaaaa tcactgggaa aacgtttgag acaattgacg tgacagcaga
     2881 ggacgattct cgaaaaggac tgagacatta ctttttacgt gtaccgatac gaaagaaaga
     2941 tgactacttg agacgtctca cgcatataga agattttcat ggtctgattt tctttaatca
     3001 actgactgaa ttaggcgtaa tggaagaaaa attgctgtat agaggtgtac cggtaggaag
     3061 tctggcctct gatcagaaca agctactccg aaaagcagcg ctagaacaat tcaaaaagaa
     3121 aatggtcaga gcacttttga ccacagatgt ggcggctaga ggtttagata ttacaggtct
     3181 tccttatgta gtgaatgcag aagttccgtt aagtgaagaa gcttatttgc atcgttcggg
     3241 aagaactggt cgaatgggta atgaaggaat agtaattaca ctcgtacaag ataataatct
     3301 aagagattta aaacggcttt tacgtccatc aaatataaca cttgaagaga tttttttata
     3361 cggaggtaag ctgcaaactg agcagccaac caaagaagat gcagctcctg cagtaaataa
     3421 aaagtatgcg aaaacttttt ctggaaagga gcagaaccca gaatataaca aagaaaaaat
     3481 aagaaaaaaa gttaaaaata aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata
     3541 agcgcctttt tatgctatga ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt
     3601 tatgtgaaag aacgtcaaga agagatcttg cagctattga cagaaaacca acaaggattg
     3661 acagcaagtg atgtagctga gcgcttaact atcgatcgaa gcaatgcaag ccgatattta
     3721 agtgaactat acaaggccca tcatattgta aaaaccgctg gccgaccagt cgtttactct
     3781 ttgccaacgg aaaagtcaaa atctgatgaa gtgcatgtcg actcttctac gcaagtgact
     3841 tttgaaacac tcgttggaga aaatgattct ttaaaagtca gcatacagca ggcaaaagct
     3901 gcgattcttt atccaccgcg ggggttgcat acgattattt ttggagaaac tggtacaggg
     3961 aagtcgatgt ttgctgaatg tatgtatcat tttgcaatcg attcagagat gctttctgca
     4021 gatgcgccat ttgtttcttt caattgtgcg gattatgatc aaaatccgca actgctcttt
     4081 ggacacatct ttgggatcaa aaaaggggct tacacaggag cagctcaaga tagtcccggt
     4141 ctaatagcca aagcagacgg cggtatattg tttttagatg agatccaccg tttaccgccg
     4201 gaagggcagg aaatgctgtt tactttcatt gataaaggta tttaccgtcc gcttggagaa
     4261 agtgcacaag tacatgaagc atctgttcag atcattgggg cgacgactga atcttcagaa
     4321 agtttcttat ctacgttcaa tcgacggatt cctatggcaa tcaccttgcc gaatcttgct
     4381 tcacgttcac tggatgagcg ttatgaaatc atctctcttt ttatcaaaca agaagcgaat
     4441 cgtttgaatc aacgtatcga tatcgaaaaa gaagcgattc ttgccttcat gctttatgat
     4501 gcggaaggaa atatcggtca agtgaaacga gacttgaaac tagtctgtgc aaaatctttt
     4561 ttacattacc gtactcatca tgagaaaaag ctaatcattc gaaaagaaga actttccctt
     4621 caagtacaaa aaggattact gaaaatcaag gaaataccgg aacgtctcga ccgattcatg
     4681 gacagcaaaa gtcagtattt gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa
     4741 gatccagaac gaaacatgca ggtctataac gatattgagg aaaaagtcac ttctctttcc
     4801 gaaaccggtg tggagaacat tgatctagag accctcattt caaaagatgt ggatgcttat
     4861 ttccagacat atgtcaaaga attgactaaa agttcgattc ccaaagatct tttgccagat
     4921 gaaatctggc ggttaaccaa tcaattgtat gatgtggctg aaaaacggtt agaccgtatt
     4981 tataatgaaa aagctcgttt tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg
     5041 agagaaggtc atatgatcgt ccacccagat ttgaataacg tacggaaaaa ttttaagagg
     5101 gaattccagg tagcaatcga tctttcaacg atcattgaag aagaagaaca agttgagatt
     5161 ccttttgatg aaatcggctt tattagtatg tttctttcta tcaatctagg agaaagtgag
     5221 cctgttaaag aaaacaaagt agatgttctc atattgatgc acggcagaca gactgcttca
     5281 agcatgttgg agatggcaca agaactatta ggaacaaaag tagggaaagc ttttaatatg
     5341 ccactggtta tggaagttca aacaatgtat gaagaagtgt tacaatatgt caaaaactat
     5401 caagagcatt tgacgaatgg cctactcttg ttaacagata tgggatcatt aaacaccttc
     5461 agtgatttga tttatgaaga gactggcatt cggacgaaag ctatcacgat gacaagtacc
     5521 atgattgtga tagaagcgat acggatggca agtgttggaa gaagcttaga agatatttat
     5581 caaaatatcc agctttcctt tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct
     5641 gaaactttac agcgtaaaaa agccgtaatc gtgacatgct ttactggtga aggagtagct
     5701 gccaaattat atcagcgtat tatgccagtg attaatcaaa ataaggtaga actgatccag
     5761 atgcagtttt tagaacgtga atcattcaaa aaacacatcg atgccttatt ggaagaatat
     5821 gagatcaaag caattgcggg aacagttgat gttgattatc aaaatatccc atttttttca
     5881 gcctatgacg tttttgatga tgaaaaacta aatattttaa agcgtatagc tgatgatgag
     5941 atcccgacag ctaaaattgt cgattcactt gctggatcat taagcgatgt cttttctatt
     6001 gatgaactga tccgttcatt gcaaaagaca gttcagcaga tccagaccga tttgtcgatt
     6061 atcgtggaac ctggagtaga tactggaatc gttatccatt tagccttcat gttagacgga
     6121 ttgatcaaac aagaaagcca tcggcaattt gagaaactgt ctattttctc aaaaatttat
     6181 cgtttggaaa tggatatcgt tcgggcaaac ttgatggcac tagaacgaaa atatcaggta
     6241 aaaattccag aagatgatat cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa
     6301 aaatgaacac actaatcata ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa
     6361 gagctaaaaa gcatgaaaac gctatttcag ctcttttttt tattggcatg cttcttgcgt
     6421 tatataaaag tgtaaaaaaa taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt
     6481 agtttgttca gcagggatga gtacgagttt attggtcaca aaaatgcaaa aagcagcaga
     6541 agaaaaaggg atggaagccg atatttttgc ggtctctgcc tcagatgcag ataataattt
     6601 agaatctaag aatgtcgatg tattattatt aggtcctcaa gtacgtttca tgaaagctca
     6661 atttgaacaa aaacttgcgc ctaaaggtat tccattagat gtcatcaata tgcaagacta
     6721 tggcatgatg aacggagaaa aagttcttgc acaagcagaa aatctaatga aataaatcaa
     6781 gtatgcagtt gggggtataa caacgtggaa gatcaacaaa atctagaagc catcatgggg
     6841 ttaatcatgt ttggcggtaa tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa
     6901 atcggtgatt ttgagttagc ggataggaaa atcgctgatg cagaagaatc acttatacaa
     6961 gcgcatcatg cacaaacaga catgttgaca caagaagcac aaggaaatca tatgcaagtg
     7021 acattattga ctgttcacag tcaagatcac ttaatgacat ctattgcgtt tactgattta
     7081 gcaaaagaaa tcatcgaact ttatcgtcgc gtatacagcg aataaataca acttcttttt
     7141 tgaacggaag aagaaaaatt tttgaacgga ttttttattt tgttataagg agaactctct
     7201 ttatataaaa aacttatagg aggaagtaaa aaatgaacgg actatcaagt tggatggaga
     7261 aatacatcct gccggttgcc gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg
     7321 atgcttttat cggcatactg cccgcaacaa tggcgggatc agtcgctgta atgatcaatg
     7381 caattattcg tgatttaccg caacagttta acagtaccta tgctgacagt ttagcagcag
     7441 acaaaggaat ctttgccgta ttgaatgtca tcatcggaat caatggtttt gtctggaacg
     7501 gtacactagc aatcgccgga ttgatctttg ccttttcttg ggggtataat cttgcccgag
     7561 cttatggagt aaatgatcta gctgggggga tcgttggttt agctaccttg attcaaggta
     7621 tttcctttgg tccagcatta tcagcagcat tagatgtgaa tgttccttca aatgtcgcag
     7681 atgcaatcaa tagttctgag attggtgcaa cgattgcaga tggcacactg tcagttggat
     7741 tatggggttg gctaaaacta gatcatttga atggaaacgc ttattttaca gttatgatca
     7801 tgggagctat ttcagtcatt attttttgta aattgatgct tgccaacatt acgatcaaaa
     7861 tgcctgattc agtaccacca gcagtatcta aagcatttgc agccatttta ccagcgacga
     7921 tcgctttata cgtcattgct attatcaatt atatcgtagg tcgtgtagct gatgggcaac
     7981 tgatcattga tttggtgcaa aaatatattg cagaaccatt cctaggatta tctcaagggt
     8041 tgggagcagt attgatcgtc acagtatttg ttcaagtatt ctggttcttt ggtattcatg
     8101 gatcaaatgt attggcacca gtgctagagg gaatctgggg acaagcacaa ctaatcaata
     8161 ttgatatttt ccaaaaaggt tataacggaa aaactggtac accagccgtg ttagatgcaa
     8221 tcgaagatgg aaaagcttat atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg
     8281 gcggatcagg cggaacgatc gtcttactga tagctatctt gattttctct aaacgtgcag
     8341 attatctaac agtagctaaa ttatccattg ggcctggtat cttcaatatc aacgaaccag
     8401 tgatgtttgg tctgccaatc gtattgaatg caatcatgtt tatcccattc ttgattgctc
     8461 cagtagtagc aacagcaatt ggatggacag ctacttatct tggcctagtc gctccggtat
     8521 cgcagcaagt cacatgggta gtaccgccgt tcttattgtc attcttagca acaggagcgg
     8581 attggcgggc accgatcgta acattagtat gtatggtcgt gactttcttg atttgggcac
     8641 catttgtcat ctctgcaaac aaaatggacc ctgccgcagg cgaacaacaa taagatgaac
     8701 gtgaggagcg ggacgcttta agtttcgttc ctcataatga attttcgagg aggaactgaa
     8761 atgattacaa ttgccagtgc aatgcaacaa gatgaaatca aagaattgtt agaaggatac
     8821 aatgaagaag gaatgacttt tacatttaaa gaaaaacaag gaatcaagct gttctttgaa
     8881 acaagtgcag aagatccgga agttgcggct aaaaaagcaa aggaactaat caaagcacag
     8941 ccttggggaa cagtccttta ttttcaatca acagctgttt agaaaatggt ttaaaaagat
     9001 aggatggttg agatgataaa aaacggttta tttttatgta gtattggttc tttgatcgtg
     9061 ttgtatagtt tgaatccaca ttcgcaaagg tatgatttgt atagtatggt cacaggtctt
     9121 tttctaacag gattaggaat ctatttcttc atgaagggga aaaaaagaga agaagcaaag
     9181 gagaaaaata actaatggaa atcacgaaaa aattaaatat tccagcatcg tttttttacg
     9241 ataaagttat ggactctgtc cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta
     9301 gaaaacagct gaaaaatttg gagtacgtca aacaattctc gcaaaataac cgggctcgca
     9361 taaaaatcga agaagtcatt gaaaaccagt cttataagtt tcgtacatcg acaacgaaga
     9421 atgaattcgt cgttcattat caaatcaaac cattggacga ttcgacctgt gaggttcatt
     9481 acacagaaaa aatggagtct tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa
     9541 caatcttgat gtattttaaa cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa
     9601 cgtattaaat caatgaaaca tggtactatt tagttattga aaaatgaagt aagctagaag
     9661 gaatttttca taggaagata gatctttcct gcgaatcctc actagctttt ttgtaggaag
     9721 gggcagcaaa atgacaaaca aagtaagaga gctaggtgta tcttcgattg aggaaatgtt
     9781 tgatttagcc acttatgcat ttaatggggc agatacacct gaaaggcgag aacgttttcg
     9841 tacacttgcg gagaattctt ggaattacgg attttttgat gaggaggatc ggctaacaag
     9901 tcaagtcatg gcgacccctt ttccagtgaa tttttacgga caagagtacc taatggccgg
     9961 cattggctat gttgcttctt atccagaagc aaggggacaa ggcgggatca atcagatcat
    10021 ggaaaagatc cttgaagatt gccgtgatcg taaagtaagc ttatcttatc tggcgccatt
    10081 ctcctatccc ttctatcggc gctatggcta tgaacaatca tttgataaaa tcagttatca
    10141 actaaacagt cgtgatgtgc cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga
    10201 ttttgaagat gcaaaagctg caatcagaag aatttatgaa cagatgccgg aaaaccaacg
    10261 agctggtctg aagcgcgaag actggtggta tacgtataaa ttcaagcaaa aaggcaatta
    10321 ccagtttgct ttgcattttg atgataatga ggaagcaacg ggatatatcg tttatcagct
    10381 tgcaacgtct tcctttatta ttgcggagtg gggatttttg gatcatgatg ctttttgcag
    10441 ccttgttcgc ttcgtaagtt ctcacaatgg tgcttttgaa acatttgaat attcatgcgg
    10501 ctacggcggt aatgatcaaa attatctatt agaaaatcca tttgcttctg taatgatcac
    10561 accttatatg atggcgagga tcgtagatat ttctttgttt ttggaaaatt atccatttaa
    10621 aaagagacaa gctgcatttg ccctacaaat agaagaagat cattatgcga aatggaatga
    10681 aggaatcgtg gaagtaacaa tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac
    10741 aaaactccca atcgttcgag gaagtatcca acatatcacg cagctattat tagggtatcg
    10801 taaagtggaa gagttggtat tccaagaaag aatacaggtg acaaaagaat atgaagaaac
    10861 gttaagctca atcttgccac agcaacagcc aattcttaat gactattttt gataaaaaac
    10921 gggtgatgac aatatttttg tcaccaccct tttgtttttc atttggcatt tgaaggaaaa
    10981 tgttaaagta aatcctcttt tttcgtaaat ttgaattttt tgttttcttt tgttttaggg
    11041 ttttcttttt tgtggtcttg gattgcttct ttcgtaatct tttcccgatt ttttttagat
    11101 gaatctaagc gttctgcgta tactttttct tttggcttat cattcattgg aaaggacctc
    11161 cctacattat ttcattctac ctctagttta tcatctgtta aggactagtg gaaacgtttt
    11221 gcctaactta taagctgatt tttttagcag ataagcttgc aagtaaaaaa caaacaagct
    11281 acaattgatt tattgaaatt gttcctgtag ctcagtagga tagagcaatc gcctcctaag
    11341 cgataggtcg ctggttcaat tccagtcagg aacgtataaa taacccctgt atcctttgcg
    11401 gtacaggggt ttgttttgta tttgtgccaa aaatgtgcca aactagttat catttttata
    11461 taaactatcg aacacagtat tgattttttc tttatcctga tcttcaagct ctcgaacaat
    11521 atgaagataa gtagacattg tagtttctaa actgctatgt cctaaacgtt tcgatacgct
    11581 aagtatattt actccttgat aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa
    11641 agtaaattgc ttttcaatcc ctaattcttt taattttttg cgcaaatatt tgctgacggc
    11701 gtttgatgaa actaatccat tttttaagtt gaaaaaaaca aaattatgag ggtttttgat
    11761 ttcaaaattc tcgaataatt ctttttgatt gattttaaac ttttttaaga gatcaatcgt
    11821 atgtttatca atggaaattt tcctatttga agtttcgttt tttgtttttc cccattcatt
    11881 taatttataa ttccatgttt tattaatagt gatgatttga tcttcgaaat cgatatcttc
    11941 ccatgttaat cctagaagtc cagcaaatcg cattccagtg gcaccagcaa ctaacacgag
    12001 cataggagaa gagtactgcg cagaaaggtt ttcttctacg agtttcatta aagatttaaa
    12061 ttgatcataa tctaaatatt tatcttcttt tggcttcaat gaatcttttc ctttgattac
    12121 tgcttttcta gttggatcaa acggaattaa tccttcttct acggcatctt tcaacgacgc
    12181 tctaatatgg ttattaaact taataacggt agattttaca tggtctttag catacttatt
    12241 taaaaagcgt tgatatccca ctctatctaa atcagaaatc aataccgctg gcatatattt
    12301 ctttatgttc attaacgtat cttcgtattt tctataagtg ataggagata ctgttccttc
    12361 tttataaagt tgcatccaat cctcaaagta atctgataat aatagatttt tgcgatcccc
    12421 tttaaggcct ttgttcaagt tatattctaa ttcgttggcg gcatcttttg cttctccttt
    12481 tgtcttaaat cctgattttc ttatcttcgt gtattttccg tcatcttttt tgtatgaaat
    12541 ttcgtattgc caactatttc cacgtttgac taatcttgcc ataattgaat cagctctctt
    12601 tctctgatac aataggaact ataaagaagc ccattgtttg ggttttatta ttttttgcac
    12661 atcctttttg cttgccggcg caaggatgtg ctatttgtcg ttttaatggt ataattggat
    12721 gtgtattaga tcatccaatc agaggtgttt attatgatta aagtgatttt taaaagaata
    12781 actgaacttc cagagtttag attatttgtt gattattggt atgtctggtt ttttattgtt
    12841 gcattgctga taggtctaga tgtcatatta gaaagaaaac ttaagtaaga gttgagttaa
    12901 taaattgaat tcctaaatga gatattttgt agcccatatt ttgtttgccg acatcaccta
    12961 atactatgga gctaatcgtt ttttctaaca gaccaaaatc agataatcta tttaagctac
    13021 catctattag ctcttgtgaa tagccagaca tgctgatatt tccagtgaaa ttttcgtttc
    13081 cacgactcat taagcatgct aacaattgaa tctcaatcga tgttaattga ttcagtacaa
    13141 gaagataata ctcctcgtta tcccatgaag cgttgttaat tcgaagtaaa caattcttat
    13201 aaagattttt atacatatct attttatttt gtgcctttga tttttctacc tcgtcattta
    13261 tcccttcgaa tattccaatg agttggtcct tgttctctaa gttgaaaaca tcttctggaa
    13321 tttgatcgcg aatgttttct aatgattcgt ttaaattttt gtagaatttc tcgatgcgtt
    13381 tgaaacgctt ctcgttttta gacccaaaat aaagtgactg cacaacgcca ccaatataag
    13441 gaattgctcc aataccgcct tcaactatta attcaagata ttcttttttg gttagttttt
    13501 cattgctatt atccatagtt acctcctatt ttgtcatttc ctttacaagc attgtgactc
    13561 ctgatggcac tcttttgtat aggttttatt attgtgcaca atctaactct tagtggggcg
    13621 tggttcattt attttttact tactttacta ttaatcatat cccattgagc ataggatttt
    13681 tctatgttcg atcctcttga actaatttct cctgtaaaag ttactttatc gccctttgta
    13741 aaggtatcag cattgtttaa gtccttcgca aaaattacat atgaaacttt aggtgttgca
    13801 gaaatatcat tccaactttt atcatcgtac tctttgtctg ctataatggc tattctagta
    13861 gtcattgatt cgataacagt tccttcaaaa gtatatgttt taccactaat atatttagta
    13921 aaagctgtat tttgctcagt agctggagta atagaataat aactatctaa gaaagcaccc
    13981 cagtcatgat tatttgtctc taaaaattct tcaaattgag ttaggctttt attgctgctg
    14041 ttagatgtag tagtttgagt atcagatgat gagctttcct gtttgttatt gtcatttgcc
    14101 tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa taatcccgat aataaggaga
    14161 atcataggtg ttttcttatt tgtttttttg ataaatctta caattaacat gattaaacct
    14221 ataataaata aaacaatacc agtaaaacca aatataccgt ccattaatag atcctctttc
    14281 tcttttgata ttgaatatta taaaccaagc aattcttttt tcttaaaatc gaattcttct
    14341 tgattaataa tcccttcatc taacaactct ttatactttt taatttcatc tgcacttgaa
    14401 atttgtgttt ctttggaatt attatctaat atagattgtt gcttctctat atattttttt
    14461 aaattttcca tttcattcca atattttttt gtaaacataa cggtgttttc atctttcgtg
    14521 gccgctaata tacctccgcg actttcgttt ccgcctagca aggtaaattg tatataacca
    14581 ttactcatac ctggtttttt taattgtacg gctgatatat ttttaaaggg gatagttttt
    14641 tctcctttaa taccttgatt aactaaattt ataaatcctt ttctaataat tgaaatagaa
    14701 tcatcagtta ctttaatcaa ggtttttcct ggagatttta ttaaaatctc tttttccatt
    14761 ttttcttcct catttctgtg atatgatttt ttttgtagaa gctcatatat gaggaaagag
    14821 cccgtgttgc agcacgggct tttttaatgt tttgctgaaa tcggtttttt taataattct
    14881 tgatgtattc atacatattg ccttgcgtaa gaatattttt tcttaaaata gcattggcag
    14941 acagcataac aagtgtatct gagcttattc tgaatagaat aatactccat aaatttttcg
    15001 agattaaact gagattcatc agtcagttcg ttctcaatat agatatttaa taaaattaga
    15061 atagctattt tatctgcttc tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac
    15121 aaaactgaaa attcaaaatg agaggcgctg aaatgtgcaa gttcatgaga taaatgaaag
    15181 gcttctgcag tttcaccgta tagattttca ttcaaaaaaa tgattctggg ttttggatag
    15241 tagaaaccag gctctttcat ctccatataa actactttca aattgtattc gctcaacatt
    15301 tctttcaatt tcaaatacat acaaaccatc actccaacta ttcattttcc tctaaagctt
    15361 tagcaattgc aatcgcttta cgcattgtct ccttagatat ttcttttcca tcaaaagaaa
    15421 aaacagtatc gtcttctgat aaatccacat gtttaggggt ttctctttct tctctaccta
    15481 gaaggtagtc tacagagaca tcgaaatagt cagcaatccg acttaattca tctgaattag
    15541 gagtattatt tttccacttt gctagataac cgttagaata tccaaaatta atttctaact
    15601 gccgtataga tatctttttc ctcttcgcca agtcttttat tatttcatag gtattcatcg
    15661 ataaaacaac ctttctaaat gtttacaaaa aaagtttaga aaaatacgca gaaataattt
    15721 gactaattct gagtaatgag ctatactata tcttgtaaac agatttaaca actaaaaaga
    15781 caacaaaaaa cactattgat atgtaaatgc agaccgccaa gaaagcttta aaatcaatgt
    15841 ttttatgtct tatttaatta tgctcttagt atagagtatt actcagatac tgtcaattga
    15901 atttagaaaa aagttgttaa atttgtttac gaatataaaa gaaaggaatg agaaatatga
    15961 acacaccgca aatttttaat ttcgaacaaa acgaagttcg gacaatttta gtaaatgatg
    16021 aaccatattt tgtaggcaaa gacgttgcga gtgttttggg ttattcaaac actaaagatg
    16081 ctttgtcgcg gcatgtagat ttggaagata agatggggtc gcgaatcacg acctcaggtc
    16141 aatcaagaga gatgacaatc atcaacgaat ctggtttgta cagtttaatc ttaaaatcaa
    16201 aacttccctc tgccaaaaaa tttaaacgtt gggtaacaag tgaagtgttg ccagcaatta
    16261 gaaaacatgg aggttatcta actccagaaa aagtagaaga agctttgctt aatccagata
    16321 caatcattca attagcaact caactaaaag aagaaagaac tggaagatta atcgcagaac
    16381 aaaagattgc agagtacgaa cctaaaatct cctatttaga tagcatatta tcttctacag
    16441 attcagtaac aattagtcag attgcagcag attatgggat gtctccacaa cagatgaata
    16501 aattacttca taaactaggt attcagaaaa aagtcggtaa ccaatggtta ttgtgcaaaa
    16561 aacacatgaa ccaaggatac acaaaatctc atacaactga gatcccgaaa gccgatggtg
    16621 gcactaaaat tgtaatgaat accaaatgga cacagaaagg gcgtctattt atctacgaat
    16681 tactaaaaaa agaaggatat taccctcaaa tggatttaga ggaaattggt tagaaaggag
    16741 ttttagtatg actgacattg cagaaatcac tcaacgagat agagaaaaaa tcaaagaata
    16801 tgtcgaaagt tcaaagttct taacttacac catgcttgct gaaagatttg gaattagcaa
    16861 aagctactta tctttaattt taaacggtaa aaagacttct gcagaagcaa acagaattat
    16921 agattcgatt atcactatgt acgaattgta aagggaggaa cagctaatgc aatatctaga
    16981 agcaaaaatc ccaattccag aaggctatgt aattatctcc caagtggatt atgaggagtt
    17041 aaaaaaagct gatgatactg gtagatggat gacgttgcca gaagtgctag aacggattaa
    17101 cagaaaatat gattggttta cttctagagt tttaaagaac ccaagatata gaaatattat
    17161 cgatatcgag aaaaacaaaa atggatttgt ctattatcca gttgaaggaa gagacacata
    17221 tctattttta agaagtaaaa cacttgaatt tttagaaaca aatttttcgg aaatcttaag
    17281 gaggcaagcg gatgggaaaa tttaatagag cgctagtgtt cagcgcaccg ctaatcatct
    17341 acgctttagg actttgggga agcaggcaag cgttgatagg aacgattgtt tacatggttt
    17401 ggatttttat ggggcttgat gaagctgaag ctgagtacag agcgaaaaag ccaaccgagg
    17461 gggctgacta aaaatgaata gaaaagagaa actagaatgt atattactat tactcagttt
    17521 aattctttca ctaatttctc tattgggaag tttgtatttt tgatatcaac aattttgtgg
    17581 atttgggttc tatttagagt aaatatcatt tttgtttggt gattaaggag tttattaaaa
    17641 ttaattttcc ctacttcaaa tgcaaggtaa caagatatag cacttttagg aggaatattt
    17701 agcggtacag cttcagagta tattctatta tcatcgactg atcctccttt agcaattaat
    17761 ttcttaaatg gagtagctga ccatctgcgg tttaagttag cggatgaaag ttctaaatta
    17821 actaacatag ctggttctgt tgaatagtta gaaatgatta cttttgtata aatcatatct
    17881 gcagcgaagt aagaagcatt aagttcaact tctagttgag ggcgcattcc ttttattttt
    17941 atccctgtta aaaacgtact caatataaaa cttattattg acatccattg aaaaattgtt
    18001 aagtgaaaaa aattcaaaat aaacaccacc agtttttaac taaattatac caaaaaggag
    18061 agaagaaata atgcaagaat tagtaatttt gaaaaataaa gaagctgtga ctacgagctt
    18121 acaagtggca gaaagcttcg agaaaaaaca tcaacatgtt ttaagagata ttgatgcact
    18181 aaaaaaagat gtgtccaatt ttggacagat gtttgtagaa ggtaatgaac cagattcata
    18241 tggcagaaat cgacgagttt tcttcattag tagagatgga tttttcttgc tggctatggg
    18301 gtttacagga aagaaagcta tctacttcaa acaaaaatac attgaagcat tcaacgaaat
    18361 ggaagatgtt attcgcaaga atactgttcc tcaaacaatt gaagatatga tgatctatca
    18421 attagaagaa atgaaagatg ttaaaaaaga tgtttccatg cttaaagata ctatgcgaat
    18481 tagcggacaa caagagtttg aaattaagca aaaaggaaat atgaaagtta tgggagttct
    18541 agggggaaaa gaaagccgag cttatgaaga aatcagcaaa aaagtattct caaaattttg
    18601 gtctgaattt aaacgtacct tttcaatccc aagatatggc gagttacctc gtaagagatt
    18661 cgatgatgct gtttcattta ttgaaatgtg gttgccagaa actgcgatcc gtatggaaat
    18721 tgatcaactg aacagacaac agagactttt tggtgatgac aatgaataga gctgaagcgc
    18781 taagaatagg gacggcaatt gctaattgct ggtggaaata ctataaacca atcatcctaa
    18841 gccaacaaca tattgacaag caaaaagcat ggcaacaaat aaaaaagtga ctccgccggc
    18901 aagcaaagag tcacaaagaa aacacatcat aaggagattt tagcatatgg aaaaagaact
    18961 ttccactcta gatcaatatt tgattgatcc tgattggggc aagccgaaaa ttgaggaaac
    19021 aagtggtcga aaaatcagac gaaatctttt gacgaatgaa gaactagctt gggatcaaga
    19081 tgatttaggc aaccatgtaa ctatttggga tcatgtttat cttatccatc tatcgaagca
    19141 ttcgaataaa cctgaatata tttacgtcat cgaagatggc ttgattgatg cgctagaaga
    19201 gtacgacaga gataacttga ttgatatctc ttattacgga ccaggtaaga aatacattgc
    19261 tgaaatggag gcagaatttg atgagtgaaa tcaaagggac aacgaacttt gaaaaacttt
    19321 ttagtcgtaa gttaaataaa attctcaaga aaaaaggaaa ttttgattat ttatcttggg
    19381 ctcacgcgtg ggagattatg aaaaagaatg atccacaggc aacggtaact attaatgagt
    19441 acaaacacta cagggttgtt tctggaactc atcaagactt tcttgttgag gaatataaac
    19501 cttttcttat ggatgaaact gggacttatg tatctgtctc agtaacggtt aaaggacaca
    19561 cggaaaccga attatttcct gttttagatt atcgaaacca accagttgtt aaaccaaatg
    19621 caatgcaaat caataactca ttgaagcgat gctttgtgaa agcattggct ctacacggac
    19681 tgggattata tgtatttcaa ggggaagata ttccaacacc acctagaatc gatacaaaga
    19741 aattaaacat gctagagacg attctagaag ctttcaatga gcagatgggt aaagatatga
    19801 ccaaaacctt aatcgaatat gttaatgagc agacagataa attagggctc ttagctgata
    19861 acgttgaaac tattgaacag ttaagctatg agcaatgtgc cttgatggag cgagcaatag
    19921 cagctaagag aaaagaatta gataagaagt gatatgagtg tttaaaccat taatcgattc
    19981 atattcagcg gttctgaaaa agttcaaagg aaaagacata agcgcaacca tcaatgagga
    20041 agtgaacatt gatcgactaa agacgatgta tgacggctac gatggtgatc gagtcattga
    20101 aattcgtttt attgatccta gacgtttcac cgtacagcaa cgaaacttca tctatgcgct
    20161 gataggcgat atttttatcg atacaggcat gccaacggac ttctggaagg aattcttcta
    20221 ctttcgtttt gaaggtgtca cagggcgcaa aataagcctg aaagacgaat cgaatacaac
    20281 tgtgagtgat gccaatgtct tagcaaatat catcttagat ttcatctttg aacatcatat
    20341 tcctttcaaa gaaggctatg agattttacc tgcgaatcaa gaatattact tctacaaatg
    20401 catcacaaaa agagtctgct gcatctgtgg caaaacagga gctgacatcg atcactttga
    20461 caaagcgcta ggaagacgaa agcgcaaaga agttgatcat gcagagtaca catttgcagc
    20521 actctgcaga atccatcaca cagagaagca caaaataggt gtgattaatt tcaaaaataa
    20581 atatcaaatc aaagggatca aattaaacca gaaaacaatc aaaaagttaa atattggagg
    20641 ggaaaaatga cagaacatcg aagttattat gcgattatac cagccaacgt aaggtacgac
    20701 aaaagactta aaccaaatac taagttgtta tacggagaga taacggcctt gtgtaatgaa
    20761 agaggctttt gttgggcagg caatgagtac tttgcagatt tatatggtgt gaataaagag
    20821 accatatcgc gatgggtaag tgatttgatt aagtttggat acttgaatcg ggaaatcatt
    20881 tacaaagagg gtaccaatca aataatcaat aggtacctac gaattaatca ataccctatt
    20941 gacgaaaaac gcaatacccc tattgacgaa aaagtcaaag ataataatac atctattaat
    21001 aatacattta ataatacaaa agaatatata agagagttac cgccttcgaa aaaatcgaag
    21061 gctaagcccg tccgtcataa atacggagag tataaaaatg ttcttttgtc agatgagcag
    21121 atggagaaac tcaaaacaga attccctaat gattaccaag agcgaatcga acgactgtca
    21181 gagtattgtg aatcatctgg taagacttat aaaaactatt tggcaactat tcgaagttgg
    21241 gcaaggaaag aaaaaagtga acctaagaac gcaagcagtg gatacaagcg cacaggaaga
    21301 cgagagaagc ttcctgaatg ggcaatcgac caagaagcct atctcaagaa aaaagcgcta
    21361 gaacgagcta atagacaatc aaaagcacca ttctaagagg tggaaaaatg aagatcgatt
    21421 atctagaact aattaatgaa atagcgaatt ataaaaaggg cgaggaatta gacgtcctga
    21481 gagacgtata tgatcaactc gaagaagctg gaattgaagg aattaagaat gatcgttcga
    21541 gttggagtaa actcagatac tatttcgcac tctatatcga tggaacgcaa ttaagaaatt
    21601 tagcatatac caaattacta ttcattgatt gtgttaaagg cttgcaaaaa catcttaatg
    21661 aacttgagca ggtgtaaaca agatggacct aaagacattt acagcacaga tcgaattaat
    21721 gcatcaagaa gctttaagac aaagtgtgtc gtacgaagac aagtggctca acacatttca
    21781 tggcggacgt gagagcgcac ttgatcaagt actcaaatta ctgaaaggag aatgtcggga
    21841 tggataagaa agcggcaatg cagcgaatta tcgaattgac ttattcagaa gattggcagg
    21901 aagataaaaa aatagttgca gaagtccaaa agctcggtaa atcaatgtgg actgaaaagc
    21961 ccaaacggag aacgccgaga aagattgcaa tctggcatgg tgatcgaatt ctagtaacgg
    22021 gtaccgctaa acagttatct gaaattactg gtctgagcaa aaatattatt tgggatagag
    22081 ctaggagctt atggattgat tcaaaaggaa gacagtttag gtatgtggag gagaaataat
    22141 ggacgaacta atcgcgaaag tagagcagtg ggcaaaagat aagggattgg atcaagcgga
    22201 tttcagcaag caaatgttaa aaacggtcga agaaatcggg gaagttgccg cttctctagc
    22261 tagaaaagat gaacatggtt taagagacgg aattggcgat gtagtagtaa ccttgatcat
    22321 cttggcaatg caaaatgata tggatttata cgagtgcttg gattttgctt atgacgaaat
    22381 caaaggtcgg actggaaaga tggtcaatgg tgttttcgtt aagtccagtg acctgaaata
    22441 attccattaa tggaagaaat aactgaatag gaggatagac atgattgatg caaaacaagt
    22501 gcaaaaacag aaagatggaa tgttgatgtt tgaagcatat gttctaccat ttcttaatca
    22561 atttgaagtt ttggaatgct ctgccagcgg agaagaattg gagtatgttg taattcgtga
    22621 aaccaaagaa aacgttcaga agctaaacga atttctttgc actattaact gttgggatat
    22681 gatagctcca ggatttttat gtccagctat gggagaattt ttggaatatt gtcgtcttga
    22741 agatgcaggc actttggatt tagcttatct ggtatacaac tacctaaaca tcaacacaga
    22801 tcatttgtgg tttggcactg ccgaaagaaa atgggtagtc cgctaaccac caatatcaat
    22861 gaaaaaaggc ggtttcctga aagtggaatt gccgccattg ctgtaaggtt ctttacgcga
    22921 ctcaaggtca cagaaatata aatgcaaatc tcttttgaag tgatccaaag ctctcggaaa
    22981 atggaagttt tttgtttcaa agaataaatg agatttgcgt caagaacctg agagttattt
    23041 ccaaaaaagc ttccaaagaa ccgaatgagc taaggacatc atgtgtaaac acataatata
    23101 gctctccttt cacggctaat cataacatga aaaatattcg atgtcagtag gaaaaaaggt
    23161 gaattatgaa aagaatatta aattatcccg gcagtaacag attctgcaat taaactccga
    23221 agctgatgta tcagtatcaa ctgtagaata ttatgagcgt ggagaaagtc aagcagtcac
    23281 aaatgtaaaa atcgataacg tcaacggtct tatctgttta gatgaaggcg atagtatgaa
    23341 tgaaattgct gcagaccatg aattatcaga aactgcttta attgatcaaa tcgaattagc
    23401 tagacaatac tttgttccat atgcagatac ctggtccaag cataagaatg acatcatttt
    23461 tccagaggaa caatgagagc aactgatcca gtaattatcc ttgaggaagc caaatttatt
    23521 tggactcacg aagagataga gcaagcacgc ttgctctttt ctcaaggagt taagccgagc
    23581 aaagtagctg aaataatggg tcaaaagatt cttgatgtcg gattgctttt gctccatcta
    23641 gcagaaaaaa tttgatttga ggtgaaaatg atgattcaac ttgcaggcat ccaaacaggg
    23701 aaaatttatt tttctggaga aagcaaaagc gaagccaacc aatggctatt gaaaacttac
    23761 acgaacaata agaagctaag gaaaaaatat cctgatttgt tgctaaagga tgatcagatt
    23821 atgccggaac cgatgatttt gacccgtaag gagggaagtt gaaactaaaa aaccagccgt
    23881 gtgggctgga gttgctattt ctttttaata atgagttttt ctaaatttaa ttcattctca
    23941 cgagtttgcg tccaataatc agaataatat tttaattgtt taagagtagg aatgtcgtct
    24001 tcggtaaaat tagaaaataa ttctggagaa ttatcttttt ttaattgttc taatcctgat
    24061 tctttaaaac gttgttcaac gagttttact aaattgttga tattcatgat aaatcacctt
    24121 tttaaattta atttcgattt gattaatcga tacaagtatt atatcaaata aaaaagctga
    24181 tttagggggt cttacaaaaa cactaaaatt ttttcaaaaa gaggaggaac ggtggtgaaa
    24241 gtcagagaac aattcaagat agtgagcgac gaaggtaatg tttacaaaat aaagatcgtt
    24301 aatattaatg aatatcgaga agcagatcac aaatatttgg ttgacttata catgaacgat
    24361 aaatatttgg gagaaggatt ttgtggagat tcactttttg atgatgagcg aattttgaag
    24421 gaggaaacag aatgatccac gaactaaaaa tactaccaga atattttgaa gcagtgacga
    24481 gcggacgcaa acaattcgag atccgcaaga atgatcgtga cttcaaagtt ggtgatcagt
    24541 taatactacg tgaatataaa aaatacatta aacatgaatt acctggacta acgagaccaa
    24601 catttagtta cactggtgac tcatacaaag cagagatcac ttatattact gattacatgc
    24661 agaagtccgg atatgtagtg ttggggatta aaggggtgaa ttaaatgaaa gtatacatct
    24721 tagctatcac agagggcaca tggatgttcc cagtcggttc cggaaaaatc tataaatcaa
    24781 aaacagctgc ttataaagca tttgaaaaat acaaaaaaga aaacggtggc ggcactaacg
    24841 caaagattct agtggctgat aactggcatg aagaaggtga acgtaattga tttacctgtt
    24901 cattttcgtg gtactaacta tttttatgct agtaatctta ttcaaaaaaa gatgagtaaa
    24961 gacgaacgaa aaattctggc caacttttat ttagtattaa aaatcgaaca aaatgtgttt
    25021 tcggaaagga gaattgtatg ggaaaaacaa aatcaaagat taagaagaaa aaacgacgtt
    25081 tggaacaaaa ggcaatccag aatggaacgg ctaagaaaaa ataaaaaagc tacccctttc
    25141 gagatagcag cgcaatacta ttctaccata aggggtggcg tttgtgagat ttcattggtt
    25201 aaaagattac caagaactag atgagcagat tctttactta aagtggaatc ttaataaaag
    25261 taagcttgaa ttgaatcgat gggtctgtgg tgatttagca aacgtccgca tcgaaaagaa
    25321 ttcgagaact tcttctttag aagagaatat ccaaaagata gaaaatgaat tggaattgtt
    25381 gatcgagcaa aaagaagaaa tgttattgct gatagatagc ttttctggta tcgataatca
    25441 aattgttaag atgaaatatg ttgatcaaat gagcttagaa gatattgctg aatctgttgg
    25501 gtatagctca tcgtatgtca gacagcgaca tgcagaaatt agaaagacat tgaacttttt
    25561 agatgagtat gaacacagac aagctgaccg attgaaaaaa gaaaacgaaa ttgattttta
    25621 caatagcgag aagtacaaag aacaactctc tttattctaa aattacactg ttcactaaat
    25681 gttcggtcat tgtagctatg taaacattgt tagcagcatg atattctatt agtgtcaaaa
    25741 aaatatgaaa gagccaagat atcccaaccg ttttattaat tggtatctgt ggctctttta
    25801 ttcttttaat tagtagttgg cataaagtta tttccattta taaattgatc agctaaatca
    25861 gcatcataat cagccgcgat aaattgcgct gctgatttaa taaatgcatc catatcatga
    25921 acatctttat tagtatgtct acgaacatag tgcgtttcat cattaccaat ccaagtagca
    25981 gctttggcta atctttggag tttaggaaaa tcattcaaat aattttcaat tgttttacct
    26041 aataacattt tttttatttt atcttcatcc tctgaatttt tagatattgc ataatcttta
    26101 attagaaatt ctagagcttt tctataacca actccagcaa tttgttccaa ttgctcagat
    26161 ttagcttgtg tggcttgagt ataaatatca acaaatgtag gagaaacttt ttcaatattt
    26221 tctggtaaat ctattttaat cggcggacgg taataatctt taattaaaat attttcagaa
    26281 tatccttcat cttcattaaa cgtatttata aattgtaaac tataaaattt gttgcactca
    26341 tcatttatac actgtactaa tacgccaaag attctatcat tatcgaacgc actatatgat
    26401 gaaaccccaa aatacacata cggagtcatt atatttccgc aatgcggaca actatttggc
    26461 aaataaattg tctctgtatt ttgacgatca cgatacttaa tattaacttc ttgaagcatt
    26521 aaaacacctc gcatcttttt tataaatcat accacaaaat atctgatagg agagtaaaca
    26581 aatgaatgat tttcatgaag cggtacttag tattgaagta gaatcaagtt tggctaaagt
    26641 ttataagaaa gcaattgaag cagagaatag tccgtacaga gaaaattgga atggtaatca
    26701 tgcacatgtg caagtggaga atgatgatta cagaactgga atgaatacgt tagttatttc
    26761 tttattgtca cacactctac ccaatctgca ggaaacgatt gaatggtatg agcgaatggg
    26821 cgcaaaagta atccgtacaa attacaaagg agagaattaa aatggcaatg atcaaaatta
    26881 gaacatccat tagtggaaca gagtattggg attcagaaaa gaaaaggaca gtagttgttc
    26941 cgaaagatca agaaccaaat tttgaagtaa aagaagaaaa aggaattttg agtgatggca
    27001 aatcatttgt tgctgtcaat ggcgaactca tagctcatag cagtgaaatt cttgatagcg
    27061 atggaaacac tgctgctaat tttgatggag acgaagctac taatgatcag tccactgaag
    27121 atacggatga actggacaat atgactgcaa aagaattgcg tgcatatgct aagaaacatg
    27181 gtattgatat tcctggtgct atccgtgcaa aaggcgatat cataaatctt attcgtgaag
    27241 tagaatgaaa tactgtcagt ttgacggatg cacgaacaaa atagcaaagg gtatctactg
    27301 tactgaacac aagagatcaa gcagatcacg caagaagaag cagcaagcaa agtctgttta
    27361 tcatcatgag aacaaaccat tctatcgaac gcaagcatgg aaagatatgc gtcaatttat
    27421 ttatgaaaga gaaggtggtc attgtcagcg gtgtggtcag ttcatatttg gaaagagagc
    27481 acacgtccat cacattgtac caatcaaaga caacgaactg cttaagcttg atccaaacaa
    27541 tctcatgcta ttatgttcaa aatgtcatcc aattgttgaa aacgaaacgg aagacaaaaa
    27601 agtttttcct tcgtatttca attgaagccc ccctattcat tttcaaaatt ttttcgcgtg
    27661 gggagatagg gtagcgggga gtcacgcgca tcgttaggtc aaatttttca aaaaacaaag
    27721 gggggtgtat acaaaaatga cgactaaagc gcaacgtaaa gcgattattg atgaaaaagt
    27781 aagtgctgaa aaagctcgta tcttagaaat aatgaatttg tctgatttgt acaccatcac
    27841 tcttgatcca ttaatcgaat catacttgga tatttttgaa atttaccaac acaaatattt
    27901 attgtggaag gaaaaaggat ttccggagac gcaaaaattc acgaataaat caggagctac
    27961 taatcaatca aaacatccat tggcacaaca agtagaaact tgggcagata agaagatgaa
    28021 agctctagat ttattaggat taacaaacaa agcaaaaact ggtagactaa ttgctggagg
    28081 atcgaccgca agaaaagatg aagaaattac acgcccagaa gtaaagccag tagatgaact
    28141 agcagcgcac agaaataaat ggcgtaagaa ggcaggtgct gaaaaatgat tgaacctggt
    28201 gtaaattatg ctgatttatt tgcaaaagaa gtaagaaaga aacctaagaa gtatcctaag
    28261 accgttcgtt tagcaataga tcgctggtat cagtggaaaa aaagaaaaga tatttggttt
    28321 gatgtagatc gcgcgaatga aatgatggat tgggtagaat catttattgt tcacacaaaa
    28381 ggagaaatgg tgggaaaacc gtttctttta gaaccctggg aaaaatttat ttattcttgg
    28441 atttatggtt gggttaaaga aaatgaaaaa ggccaaattg tacgcgttac tcgtgaggct
    28501 tatgtccaga ttccaaaaaa gaatggtaaa acattgattg ctgtcggttc attaggatat
    28561 gcaatgtacg gagaaggggc gttatcagtt gattgctatg cttgcgcatc tgattttgct
    28621 caagcgcaat atgctgccaa accttttgca gttacaatct taaacaatcc aatcctactt
    28681 gaaggaacta aaatatttag ggggccaaag ggaactgttt ctagtattac ttatgactat
    28741 attcatgaag atatggctta ctcaaataaa tttattgttc aaaccaagaa tattgataat
    28801 atcgagggat ccaatccata ttttgtattg aacgatgaat tgcataaaca ggagaaaatg
    28861 gaacagtatg acaattttaa gtctgctcag atatctttac cacagccatt gatgtttaat
    28921 atctcaactg ctgggaaagg ttcgtcgtct gttggaatgc gtgtatatcg cgaagcaaaa
    28981 gaagtgttga aacgtgatga taatgattca aactttgttc taatctatga gccaaataaa
    29041 aattatgatt ggacggatag aaaagtctgg gaaatgtgca atcctaactg gggaatatca
    29101 gtcgatttat ctgctttaga atcggcattt aaaacagcgc aacggtcagc tcattccaaa
    29161 gctgaatttc taacgaaaca cttggatgta tttgtgaacg gcgcggataa tttctttgaa
    29221 caggatcaag tagagccatg tttagttacc acacaagaac ttggtgattt aagtggcgaa
    29281 ccatgttata tcggtttaga tttatcacgt acacgagatt tgacctgtgt atctttaaac
    29341 ttcccaacat gggatgaaga tggtaaagcg gttcttaaag taaagcaact ttattttatt
    29401 ccgaatgaag atttagagtt tcgagaaaaa gaagataatg ttccttacag tgatttggct
    29461 gaacaaggtt tcgttgaatt ttgcgacgga aaaatgattg atcaagatca gattttgcag
    29521 tacatagagg actgcatgaa tttatatgat attcagcaag tgaattatga tccagcgatg
    29581 agtgacaaac ttgttgagaa attagaaaac ttaggattgg aatgcgttga agttgctcaa
    29641 tatcctaaag tattgaatgc tccttttgac gatgtcgaac ggttgtttta tgagaaacga
    29701 attcaatttg ataatccatt attcctttat tgcactttga acgttgtagc aattactaac
    29761 atcaatggac aaaaagcgcc aagtaaacgt caatcaaaga aaaagattga tgggttcgtg
    29821 gcgtttttgt gtggtcataa ggaaacgatg aatcaaatga cagatattga ttcagatgag
    29881 ttagatgatt acctaagttc catctataga taaatagaaa ggcggtgaaa aaaattgaaa
    29941 ttacgtgatc ggttatcgaa tgcagtctat tcttttatgg aaaaacgtgg atatatcgag
    30001 gatatttttg gacactacac acgttatggt caaagatatg tgacggattc atctatcatg
    30061 gaatcttctg atgtttatga attagttcaa gacatatcta atcaggtggc attggccaca
    30121 ccaattgtta ttggtcccga cggcaacgaa gtcaaagatc atcacttact aaatattttg
    30181 aagagtccca atgattattt gacaggattc gaatttacca aattggaaac aaatacttta
    30241 ttgatcaatg gtgagacatt cccattaacg gatagggatc agcttcattt ggcgtacggt
    30301 gtaacaacta aaatcaatga acgacttcaa gaagaatttg aaatgaatgg tcaaaaaata
    30361 cctggtcaaa tgattcgaca cattaagaac atcggaactg attcattaaa aggtgctgga
    30421 ataattgatc ttgcaagaaa cactctagaa ggcgttctga gcgctgaaaa agttttgacg
    30481 gacaaatata ctaaaggcgg tttacttgcg ttcatgctta aactagacgc ccacatcaat
    30541 ccaaataata gcgcccaaac aaaaattgtc aaagctatat tggatcaact ggaaggaacg
    30601 caaaatgaga gtgatcattc tgttaagatg attcctttgg gaaaaggata ttccatcgag
    30661 acattaaaaa gtcctgttga tgacgcggca attttgaatt atttaggtgt ttacaaaaaa
    30721 gacttaggaa aatttctagg aatcaatgtt gatacgtatc aatcgctgat gaagacagat
    30781 attgaaaaag cgatgatgta tctgcacaac aaagcaatca aaccaatatt gaaaaacaag
    30841 agcgaacatt acaccgctct tttttttatg cctaattctg gctatagagt ggaatggaaa
    30901 attaatattt tggattttgt tccttactca acaaaaacaa atattggcta caacatcgtt
    30961 cgtaccggga ttacaagtcc agataacgtg gcagaaatgc ttggttttcc taaacagaat
    31021 actccagaaa cacaagctat ctatatttca aatgacttat ctaggattgg ccagaaaaat
    31081 gcaacagatg attccttacc aacgaatgat caagacttga aaggaggtga tggaaatgaa
    31141 gaagaaggaa attcgcacga ttgacatcac caatctttca acacgttccg atgaagaaac
    31201 tcatacgagg accattagcg gctacgctgc tgtattcaac agtcaaacac ttttatggga
    31261 tgacttaagc gaagtgattg ttcctggtgc attctcaaag gcaattagta attcggatgt
    31321 tcgttgtttg ttcaatcacg attggtccaa tgtgctagga cgtactaaaa gcggaaccct
    31381 tcgccttgaa gaagatgaac gtggtttgaa attcgaagtt gatttaccgg acacaacggt
    31441 agcaagggac ttagttaaat ctatggaacg aggagacatc aaccaatgta gttttggttt
    31501 tgtgccgact gaagaaacgt gggactacaa ttctgaacca atgcttcgaa caatccatga
    31561 agtggaatta tacgaggttt ctattgtgcc tttgccagca tacgaagata cagaagctgc
    31621 attaagaagt cgtgatgaat tagaaaaaaa cgtcgaagaa agaaaaaaat taatcaaaaa
    31681 aattaatcaa gcgctagaag cgtaggagga aaaatacatg gataaagaat tattgaaaaa
    31741 aatgaaggca cgtcgtgagc aacgattgac tgaattacgt gaaaaagttg aatcaggaga
    31801 attacgcgaa gcagatttag aagccgtgaa agaagaaatt gacagtgtta ttgatgaatt
    31861 gaacggaatt aaagacgaat taggcgcaga ttctggaact gatgaaacag acgacaatac
    31921 agatgatcaa tcgaatagta cggattcaga cgaaagccgt tctggtgaag acaacgatca
    31981 agaagaagat tcagacagtg aagatagttc agaaaatcgt tctggaatga ttactcaaca
    32041 gcaacgagat ggattacttg gatcaattaa gaacggattg gaggcacgtg caaaaatgac
    32101 caagaaacaa aaagatcaac aactacgaaa agcatttgct aattttgtag ctggaaatat
    32161 ttctgaagca gaagctcgag ctttagggat tgaagctggc aacggttcag ttactgtccc
    32221 agaagtaatt gcatctgaag ttattactta tgctcaagaa gaaaatttac ttcgtaaata
    32281 cggaacagtg gtgcgaacat caggagatgt caaatatcca attcttgtga agaaagcaga
    32341 tgcgaatgta aacaagaaag agcgttcaac tgatattgct gaaacagcta ttcagtttga
    32401 tgaaattttg cttaatcctg ccgaattcga tgctttggca acagtaacta aaaaattatt
    32461 aaaaatgtct ggtgttccag ttgaagatat tgttgtggaa gaattgaaaa aagcttatgt
    32521 gcgtaaagaa atcaattata tgttcaatgg tgatgacgct ggaaatgaaa atcctggtgc
    32581 attagccaaa aaggctgtag catttaaaaa acctttagat ctaactgctt caggtgctgg
    32641 gcaaaaatta tatgatgcat taatcgaatt taaaaataca ccagtgacag aagtgatgaa
    32701 aaagggacgc tttattatta atcgagctgc tttgactgcc attgaaaaaa tgaaaacaga
    32761 tgatggattt cctttgttgc ggccattcac acaagcagaa ggtggaattg gttaccaatt
    32821 agttggctat cctgtggatt ggacagatgc agcagataaa aagggtgaac cagacacgcc
    32881 agttttatat tttggcgatt tttctgcatt taaaattcaa gaagttattg gtgccttgga
    32941 aattcaaaaa cttgttgaaa aattctctgg taaaaatcaa attggattcc aaatttacaa
    33001 cttgctagat ggtcaattgg tttattctcc atttgaaccg gcagtatatc gctacgaaat
    33061 tacaaaacca gttggtggtt aagatggaag agcaaactaa agaattgtct ttagaggaaa
    33121 aattcaaatc acatattcat tttgaagagg gcatggatga ttctttgctc tctttttatt
    33181 taaatatggc aaaagattat gtcaaaacag caactggtgg ccaacaagaa tatcttattt
    33241 tgatggttgc cggcattgcc tatgaatata gagtttcaga ggatgaactc gacaaagcta
    33301 tgaatgccat gacgccattt atcgtgcaag gagcgattca aaatgccgaa gagacagacc
    33361 aataatctca gatggaaagc cgaattgcta gacatcaaaa caggaacaga cggaaacgat
    33421 cgtccaacta caatttacga atttaagcgt ctaatattct atgaagaact cggtgtgact
    33481 tctcaagaaa aatatttatc acagcaagcc aagacagacg ttgtcagacg aattaaagtc
    33541 agatgggata aatccatcac agagaaatta agtgcgctca aaattgattc tgtaacgtat
    33601 aacattactc gcatttatac gaatcccgat gcaagagaaa tggagttgag tttagcttat
    33661 gtcgattagc tttgatgaat tgaaaacagc gctgaaatca acaaagttag cagtgttcag
    33721 agacaaagcc agattaggga cgatgtatcc atacattgtg tactcaaatg tgagtaacag
    33781 caaaaaaatg gcatccggta aagtctataa aaaattacct tattaccaag tctctttttt
    33841 tacacttgga acagaacagg atttagttgt tattgaacaa gcgctgcaaa atgctggcat
    33901 tccatattca gattttacag gtatacaagg tgatgagaac gacgataccg taaccaacta
    33961 ttacacatat gtgaggtgtg tggaaaatgc caagtaataa gaatggtttt tctgaaatat
    34021 cggattattt aggaaacctt tcaaaagttg atccgaaaaa attatcgttg gaatctttag
    34081 aagaagcagc taacttttat ctaaagcagt tgcttcctaa tattcctaag tcgctcttaa
    34141 aaaagaaaca tatgcgcgat caaataaaag tagtagttga aaacgatcaa gtgaaagttc
    34201 aatttgaaga gacggccttt tactggcgct ttgccgaaaa tggcacaaca aatcaacgtg
    34261 cccaacattt tgcaagtggt acgtatgaac aaaacaaaga aaaaattgaa gaaattatga
    34321 ctagaaaaat acttgattta tgggaagggt gattttaatt gggaaaacaa gatacttttt
    34381 attttgaagg attggacgac attttaattg cgatgatggc aacgccagat tctgttggaa
    34441 ctgcaccgac ttatagtgaa gttttacgat tgccaattgc cacaaaaatc ggtgtcaaag
    34501 ggaatggaac agcattagaa aaatgggcat ccagcaaaat gtttcgtcgt gtgtcgcgag
    34561 aaacaaaaca tgaacttgcg ttggatcacg tgggtattcc gatagaagtg atggatgaaa
    34621 tcaaaggatt agttgctaaa agcggagtaa cgtttagcaa aaataccgca cgagaatttc
    34681 cgtacttcgc ctttggattt attggaaata ttgaaaatgg cggtaaaaaa gcagtttggt
    34741 atcctaatac acaactatcc aatgtgattg acgaagagta tgccactgca gaggaagaaa
    34801 caaaaattga cgatgtaact gcaaatctgg tttccatcgg actgaaatac aataatgtga
    34861 tgtatgcaag ttttgattca aatagagacg gagcttctct agaattattt aataaattca
    34921 ttgctcaacc tgtatacgat gaagaacagt ggaaaacatt ggctaaagtt ggaggtgcgg
    34981 gttaatggct cggttatctg attatggaat tcacgttgaa gacttaaaaa attctgctgt
    35041 tgtaattatt caaggcgcag aattccctat ttcatttact atgcaaacga tggaatttat
    35101 agcagatgtg tatggtggag attattcgca atttgaatct gatatgaacg ccatgctatc
    35161 taaaaaagaa ggacaaattt cttctgctaa cttatcgcct agtgatctaa aaatcatgcg
    35221 tgccttgatt tatgccatgc tgcgtactgg tggtttagag gaagatccag aaactatttt
    35281 caaatttttg ggaatgagtg gagaggtgtt gtctgcttat agtacctgta tggaaatttt
    35341 tgctagccag acatttcagg tggaagacct aaaaaaatcc aagaagccac aagactttca
    35401 aaaagcgcaa gcaaaaagaa agaaaaacaa aaagaatcgg aagagatagg aactccttgg
    35461 agtttttata tttacgttgc tctcactcta ttgaattgga gtgagagttt ctttttaaag
    35521 tctacaccta acttgtggct caaatcttat ttacagtggt tacaacaaaa caccgatttt
    35581 gaaccacctc aatctgtaac tatggataaa tcgccttggt ggtagaaagg agcgctaaga
    35641 taaatggctg gtaaagaatc tgatgtcgtt cttaatttta aaacgaatgg cgaagtcagt
    35701 tattcgaaaa caatcaaaga aatcaacaaa gaaatgaact tagccgctgc cgagtacaaa
    35761 aaccaagtgt ctgcgatgga caaggatgca actcaaacag aaaaattgcg agcggctaaa
    35821 caaaaattag aaaaacaatt aggattggct gaacaacgat ctcaaatgct gagagaggaa
    35881 tatgaaaaat ctgtcaaaga aaccggtgaa tattctgctg aatcagaaaa actttataaa
    35941 caattactca attctgaaac cggagaaaat aaacttcgta cagcattaga acagacgaac
    36001 gatgcactta aagaacaagg tgacgtttct gttgatacag caaaaaaact ccagaaaatc
    36061 gaagaaacag gtgagaaagt aaaaggcgtt ggtgaaaaaa tgtctgttgg agtaaccgcg
    36121 cctattgtag cggcaggagc agcaggactt gcagcatttg gtgaagttga cgaggcactt
    36181 gataccatca ttacaaaaac cggagcaaca ggtgatcaag ctgatagact ttcacagtct
    36241 ttcaaaaacg ttggttcaaa tactcattta cctttacaaa cggttgggga agctattggt
    36301 gaggtaaata cacaatttgg attcatggat aaaaaactgg aagattcaac caattatctc
    36361 ctacagtacg ctgaaatcaa tgatacagat gtttcgcaat cagcaatatc tgctcgacaa
    36421 gctattgatg cttatggact agaatatgat gatttgaatt ctgtccttga tgtaacaacg
    36481 aaaacatcgc agaatactgg tcaatctgta gacgacttga tgcaaaaagc aattgatggc
    36541 gcaccacaaa ttaaacaatt agggttgagc tttggtgaag gggtcacttt acttggacaa
    36601 tttgagcaat ctggtgttga ttcaagcgca gctttaagta gtttatctaa agcaacagtg
    36661 gcttatgcga aagacggaaa atctttaagt caaggactag aagaactaca agacaaggta
    36721 aaaaatgcag gttctgaaac agaagccatc aatgcagcag cagaagtatt cgggactaaa
    36781 ggcggtccta gaatggccga tgcaattcgt agaggtactt tgaacctgga agatttagca
    36841 aaaactgctg gagaaagtgg gggagctgta ggagacacat tcgatgctac tctcgaccca
    36901 attgatcaag ccgatcaagc tatgaacaat gcaaaattag caatggctga cgtaggtgaa
    36961 gcagtccaag taagcctttt gccttttttt gaaaaagcaa cttccttatt acaggaattc
    37021 tctaaatggt gggggtctct agatcaagat acaaagaatt ggatcataac aattgctggt
    37081 atagcggcag cagttggacc agtactaatc gttcttggtt cacttatggg atctgttaca
    37141 aaaatcgttg gggaagttaa aagttttata ggagtttggc aaggacttgc tggtttattt
    37201 ggaatgtcgg gaggctggtt tgcattagca gttatagcta ttggcgcatt agttactgga
    37261 ctaatttggg catataataa cgttaaatgg tttcatgatg gagtgaattc tttcttcaaa
    37321 ggtgtttcag atgtagctgt tgaaatattt aattttttaa gtggttattt tggtggattt
    37381 ttcgatggtg ttatagcaaa tttcaataat ttttataatg ctggaaaacg aattttcagt
    37441 ggttttctag attttatcac tggagtattt acagggaatt ggtcaaaagc atggcaaggt
    37501 ctagttgata tttttggagg tattttcgat ggtattgttg ctgtagggaa agctcctatt
    37561 aatgccatga ttggattgat caatggattt attggtggat tgaataatat aaagatacca
    37621 aaatgggttc ctggtgtagg tggaaaatca ttttctattt caaaactgcc ttatttagca
    37681 caaggcggtc atctaatcaa tggacaagcg attgttggtg aagctggacc tgagttactg
    37741 actgccaaga atggtaaaac cacagttact ccattatcag acgaagaaaa acgcaaaggg
    37801 atcagtggaa gagtttctgg aggtagcatt gaacagcata tacacattgg caaagtcgat
    37861 gcaaacaatc catctgagtt agatcgtatg aatcgtaaat ttgctaaagc aaacaaacag
    37921 gctattcgtg atttgggagg tgttcctatt tgagtcgaca atttatgaat ccagatgaac
    37981 caaattttat ttggaaaaat cggaatgcag taattgatat gaattgcatc attgaggcag
    38041 agcttccaga tattatgccg aacaagcgat acgaaactta cacgattcag ggccgaagcg
    38101 gagaattgac ggagacgttt gatgactatg aaccttttga tttggaaata gaagggatca
    38161 ctattccaca ttcgaaactg agagaagtca aacgatggct cactggtaaa agccaattaa
    38221 tcacacataa tgatccagac aagtatctag aggctatctg cagcatggat aaagaagtcc
    38281 ctttcgaaaa cgagtggggc ttcttttata cattcgatgt tacttttcgt tgtcagccac
    38341 tcaaaagaaa aatcggtgaa gcacctaaaa tttttcattc tagtgttatg gatatttttg
    38401 atcatggtga cgaaattgca catccctatc tggaaataga atcgaacgga ggagatataa
    38461 cgatcgctgt cggtgataaa agcctaacaa tacttaatac gttagctgca acaatcgtgg
    38521 ttgatactca gcttggaaaa gcaatacaag aaggtttgaa tttatttacg cgtggtgatt
    38581 ggccagtatt gcagcctgaa tggaatcagg taaaaatatc cggaaaattt aaagaagtgc
    38641 ggttttggaa taggagtgtg tatctgtgac gcaagaattt atttatgctt ataaaaaaat
    38701 gccagatgat ttgagcgtga acggagcgtc gttagttgat tgggaagatt tgccagaaat
    38761 caatcgtgtg ctgaatggcc aatatcgttt ttatggtaac tattcaagaa gcggacagta
    38821 tcgctcttat ttaaaaaaag gaaattttat aaaagcgaag gtaccagatg gatcatggca
    38881 atattttgaa atttataatg tcaaaaagaa tttgacctcc gtttctgtaa cagctcggca
    38941 tattggtttt atggccaata agaactttat tgtcaagtcg tttacggata acggcaatgg
    39001 gtctcagatc atgaccaatc ttaaaaacag tttggcattt gatcaaaaat ttaactatct
    39061 ttcgaatgta gggacaacgc accaatttac agcaagacaa gtcgcgccag ttgaagcaat
    39121 cattggttca aataacggta atcaaaattt gactggcgtg actagcgcgg aacttgatat
    39181 ggataactat gatttgaaac tggtaaaaca gattggatca gataatggtt ttcggattga
    39241 ttttggcatc aatttagaag caatcgaaga agaaattgat gaagaatcaa ttgtcaatag
    39301 tttgtatttg gttggcggtg ttcctgataa cgattatgat gaagacaaag aacctatcga
    39361 atatggttat ttagaaattg atggagtaac caacgagaat cgacgtattg ccaaacgtga
    39421 aaacggagat tgtaaaacag ttgatgaatt gatcaaatgg ggcaaaacct tatttgacaa
    39481 tgatcgtatc catgagccaa aagctactca tacagtcagt atggtggcgt tggaacacac
    39541 actcgaatac ggtgacatgt atcaagagct agcgtcttta cactttggtg atgtcgctca
    39601 tgttagggcg aaagagctag atatcgagat aaaagagcgt atggtcgaat acacttattt
    39661 cccaacgctt gggaaatata aggatattgt tttaggtaat gatttatcac tgtacacttc
    39721 ggcagtaaat actcaagcac aagaattgaa aaagaaaatc gacaatcgaa cagaaacgtt
    39781 agttcaaaat gtgttgaatg cgacggcatg gatcactggt aattctggtg ggcatgtcgt
    39841 ttttcgtcca gaaaaagctc catcagagat tcttatcatg gatactccaa atgtggcgaa
    39901 tgcaaagcga gtgtggcgtt ggaatttaaa tggcttaggt tattcagaca acggagtaaa
    39961 tggcccgttc ggtattgcta tcacatcaaa aggagaaatt gttgctgact tcatcaaagt
    40021 aggaacaata aatgcagaag tattcgaaac ttcctttaat gcttatggtg atgtgttaaa
    40081 acttgtaaaa ggcacgcttc aaatttggaa tgaaaacaaa aaaatcatgg agctaaccaa
    40141 aaaaggtatg gaattctgga atagaaaaga atccattgga acgatcggaa caacagattc
    40201 agcaggaaat ccatttccaa atgctgtaac acctacacct cttgaggaaa attctttggt
    40261 aatacgtaca aatggcgatg gaaaatacat tttaatttct ccaactgcag aaaaaggatt
    40321 tgtgttatta ggaaacggaa aagcatatta tttcggtgat ttagatattc agggaaaact
    40381 tactgttaga ggtaaagaag tgataccagg acaaaatggc ggtccatctg gcggaggaga
    40441 aacacctggt gggtatcctg atgaattaaa aacagatgca gaaaagagag cttggagaat
//
LOCUS       DAZKOW010000012        37908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000012 DAZKOW010000000
VERSION     DAZKOW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.012"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..3660
                     /locus_tag="ACG912_000685"
     CDS             133..3660
                     /locus_tag="ACG912_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFQ0512029.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="ACG912_000686"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="ACG912_000686"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFQ0512030.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="ACG912_000687"
     CDS             7448..7789
                     /locus_tag="ACG912_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFQ0512031.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="ACG912_000688"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="ACG912_000688"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0512032.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /gene="pheT"
                     /locus_tag="ACG912_000689"
     CDS             9146..11566
                     /gene="pheT"
                     /locus_tag="ACG912_000689"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFQ0512033.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG912_000690"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="ACG912_000690"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFQ0512034.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="ACG912_000691"
     CDS             13000..13737
                     /locus_tag="ACG912_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512035.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="ACG912_000692"
     CDS             13751..14566
                     /locus_tag="ACG912_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0512036.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="ACG912_000693"
     CDS             14579..15226
                     /locus_tag="ACG912_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0512037.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="ACG912_000694"
     CDS             15242..15898
                     /locus_tag="ACG912_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0512038.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="ACG912_000695"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="ACG912_000695"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFQ0512039.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="ACG912_000696"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="ACG912_000696"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFQ0512040.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="ACG912_000697"
     CDS             18240..18758
                     /locus_tag="ACG912_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0512041.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG912_000698"
     CDS             18865..19365
                     /gene="cbpB"
                     /locus_tag="ACG912_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFQ0512042.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="ACG912_000699"
     CDS             complement(19645..20565)
                     /locus_tag="ACG912_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFQ0512043.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20691..21743
                     /locus_tag="ACG912_000700"
     CDS             20691..21743
                     /locus_tag="ACG912_000700"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFQ0512044.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG912_000701"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="ACG912_000701"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFQ0512045.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG912_000702"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="ACG912_000702"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0512046.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="ACG912_000703"
     CDS             complement(23383..24144)
                     /locus_tag="ACG912_000703"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0512047.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="ACG912_000704"
     CDS             24333..25427
                     /locus_tag="ACG912_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFQ0512048.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..26472)
                     /locus_tag="ACG912_000705"
     CDS             complement(25513..26472)
                     /locus_tag="ACG912_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFQ0512049.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            27298..27477
                     /locus_tag="ACG912_000706"
                     /pseudo
     CDS             27298..27477
                     /locus_tag="ACG912_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27656..28606)
                     /gene="yidC"
                     /locus_tag="ACG912_000707"
     CDS             complement(27656..28606)
                     /gene="yidC"
                     /locus_tag="ACG912_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFQ0512050.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(29044..29319)
                     /locus_tag="ACG912_000708"
     CDS             complement(29044..29319)
                     /locus_tag="ACG912_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFQ0512051.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            29427..30191
                     /locus_tag="ACG912_000709"
     CDS             29427..30191
                     /locus_tag="ACG912_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFQ0512052.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            30314..30817
                     /locus_tag="ACG912_000710"
     CDS             30314..30817
                     /locus_tag="ACG912_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0512053.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            31225..31871
                     /locus_tag="ACG912_000711"
                     /pseudo
     CDS             31225..31871
                     /locus_tag="ACG912_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     regulatory      31913..31979
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            32034..33302
                     /locus_tag="ACG912_000712"
     CDS             32034..33302
                     /locus_tag="ACG912_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFQ0512054.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(33329..34528)
                     /locus_tag="ACG912_000713"
     CDS             complement(33329..34528)
                     /locus_tag="ACG912_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFQ0512055.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(34647..35954)
                     /locus_tag="ACG912_000714"
     CDS             complement(34647..35954)
                     /locus_tag="ACG912_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFQ0512056.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            complement(36070..36192)
                     /locus_tag="ACG912_000715"
     CDS             complement(36070..36192)
                     /locus_tag="ACG912_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512057.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            36425..37543
                     /locus_tag="ACG912_000716"
     CDS             36425..37543
                     /locus_tag="ACG912_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512058.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
BASE COUNT        12299 a         6613 c         7961 g        11035 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca gtccattacg
    25681 acgcagaatc ccgttagaat tctcgtttaa tgggcgttga gaaggtgttc cagggtccgc
    25741 aaaatatata tcaatatcat gttggttact aatggctttc cagttagaaa attcttttcc
    25801 acagtcaaac gtaatagatt taaagaagtt tttagggaag cgagaaaacc attgattaag
    25861 ggcagtttca atatctaatg ccttacggcc gttgggttta atcgtgataa tgactttaga
    25921 taatctttca actaaagtaa tgacggcact tttatgatga atgccaacga tcgtatcacc
    25981 ttcaaggtga ccaaactcag aattgaaatc aggataatta tcaggtcgat catggattga
    26041 gcgctgatac tgttgttttc cccgtttttc ctgatggcca ttgggttttc ttttaccttt
    26101 catcggtagt gtgtcaatat caaatattcc tttagaaaat aaacgataaa gtgttctcat
    26161 accgcatgaa acagaccttt cttttcgccc gataatgacg tcaggcgtcc aaccaagagt
    26221 gactttctct ttaatgtaat ctacttcatg agcaggtaat tgaatgactt ttctaccaca
    26281 ttttttctta ttttctttat actggtgcca ataatcaaga gcagtcttgc cttgcttgaa
    26341 cttattgact acattataaa tagtttgtat agcacgtccc attcggttag cgatttcaac
    26401 cggcttatta tgttgaagat aatatgattc tatcattgtc agttcgtcta tggtaagatg
    26461 tttgtaggtc atttatggtt acactccttt gttttctttc gtcggaaata caatttgagt
    26521 gtaccataaa tgccttttta tttttctaac ttaattttac aattcgcgtt atttagagaa
    26581 caaacttgtg taacatacaa aggaaatttt gaatatgaaa gatgaggaac taaaagaaga
    26641 tttaatagat aaattaggga ttgcactgag aatggttcga agagcagtcg caggaaatat
    26701 gtttacacaa tgagcattca acaaaaagaa tggtcttttt tcattgtcat ttttcaataa
    26761 agaagttttg ccagaggaaa tttttttaag ctggcatagt tgtccggtat gagattgaaa
    26821 aaatacagtt gtgtctgaca gctcatttga gaaattaata agataagttc atcaaaatag
    26881 attcagagcc cccttgatag agattaaaaa acagcaagag tgtctttaaa gcagctagga
    26941 aataaaaaag atggagagaa gccttatttt gctgattttt aaataccatc ctgagtaaaa
    27001 gtcagaaagg cggttgttat gcaaacattt agagatttga agaaatttgt agttgctaca
    27061 aagaaagtat aaaagaaacc aacatttaag aagttgcgga aaggagccag tttctatccc
    27121 aaaaaggtga ttcaaaacaa ttcggttaca atagcggttt ataagaaggg aaatgttgtg
    27181 tatcaagctt tgaatcgtgt aattgtattc ccagtagaaa aagggatgga tactatcact
    27241 attcttctac tagaaaggtt tatggatgtt atttagatgg gtggtatctc cgcgttgtcc
    27301 tttatggaga atggtgtctt atgtgggcga gcaacatggg cgaagggagt ggcgcctttt
    27361 gttaccgaag gcgaagaaaa agcgagtagt tggatgttag aagaaggaaa acaaaatatt
    27421 aaggaattaa gtacactatt agaagaaaca gcaacacctg tctacgttga tagataagaa
    27481 aaagttgttt cttcataaaa agatgctgct gcaattagat agcgatcaat cgatgattca
    27541 aattaaacat tcacttaaat aaaaatatcg accagatttc tttttcaaaa atcttggtcg
    27601 atatttttaa ttgctaaaac tgttaatgaa tttttattac actcttattt actatctact
    27661 tccgcttctg ctgctttcct gcgttgcggc ctttattgtt tgtactgttt ttgggttgct
    27721 tgatttgttt ggttgcttgt ttgctgtcag ctggtttagc tgattctgtc acatcaattc
    27781 ttggagtgac gacttgtttt ggtgggtttt gtttcaactc ttctgccact tgtgctttca
    27841 aacgtggttt catcataacg tttgtgatga atgtttgcag acagctgaac acaccaccga
    27901 ctacccagta aagtgtaaca ccggctggcg cactgatcga catgaagacg atcatagcag
    27961 gagagacgat cagcatcgta cgcatcgtct ttttctgttc ttcaggaata ccgatcgttg
    28021 agatgtatcc ttgaagcaga taagcgaccc ctgcaatggc aacaagtacc atacttggtg
    28081 agcctaaatc gatgccgtag aaagatgatt cccggatacc ttctgtataa cgtgccgtaa
    28141 agtacaaggc agagaagatc ggcatttgga tcaatagagg cagacagccg attccgccgg
    28201 tcatactcaa tccgttttcg cggtatactt gctgcatttc tgcttgtgct gccatttgtt
    28261 cttcacgtgt tgttgcagct ttcaattttt gttgggctgc ttcaacttgt ggttttaacg
    28321 cttgcatttt ttcagattgg atcattgttt ttttcgattg actaatacct agtggcaaga
    28381 tgatgatacg gacgatcaca gtcattgcaa taactgccca gccataagac caattgaaat
    28441 tttcaactag atatgtaatc gcttggccca taggatggac gagtactcga taaacgattc
    28501 ccgaagtgtc agggtttccg tttgaatcca tccgcacaca gccagataaa aagagcagga
    28561 tactgacaag tccggatccc aataaccagt ttttccattt attcatagaa tacttcctct
    28621 tctataattt ttacacaaac ttaactatac taaaaaagcg catggaattc aatcaacttc
    28681 atagaaattt catataaata ttcgttcttt tacgtagcga aaaattttga aaaaaagaca
    28741 aaaaatcaag aaagaaatac taggaaaaag tagaatgatt ccaagtgttt ctttcttact
    28801 ttaataaact aaaaactttt tttctgcccg aaaaagcgga gtcaaatgaa ctcactgcaa
    28861 gtgatagatc agctttttca ctgctgtcct taatttttga ggcaaagcgc ctcaagaata
    28921 accttatcga cggtgttcaa aagcttgcat ctacggcatt aagtccagcc tgttgaaaaa
    28981 tatggagagc tatttttcac aacctgtcct taatgcttga tgcagaccag ctttttcaca
    29041 accttatccc accacatcaa atttctttcg ttcctcaatc atcgggtctt cttggacatc
    29101 cacatactgc actcgtccac ttgggctcgg agatttcttg acttcttcaa taaatttctg
    29161 gatgatgtcg tcattgccga ttgcttcgat cgtgactgag ccgtcatctt cgttttttac
    29221 cgttccagtc acgcctagtt gatcagcgac catcttggtc atgtagcgaa atcctacgcc
    29281 ttggacgcgt ccttggacgt tcattttaac tttgcgcatg ttcgtcacct ttctttccta
    29341 ttttataaat taatgatacc gattttttta ggatgaaaca agctcaagca cctattccat
    29401 gatataatca tggggaggtg aggaatgtga aagagatcca atcagcaaaa aatccaagaa
    29461 tcaaagaatt aaaaaaacta cataaaaaga aatatcggga agaaaaggaa gaatatcttt
    29521 tggaagggtt ccatctgatt gaagaagctg cgtcgtctga tgcagaaata aaagaacttt
    29581 ttgtgacaca gcgaggcatg aaagaatggg gagactggct ggccgcaaac aaagagattt
    29641 ctacttttct agtttcggat gaggtcatgg atgctgtatc ggatctacct acaccacaag
    29701 gaatcttagc agtgatggcg ttaacggctg aaggcacgcc agatttttca ggaggatggc
    29761 tgttgttaga taatgtacaa gatcctggaa atgtagggac gatgatccgt acggcagatg
    29821 catttgatat ggctggtgtt ttattaggca aaggaacggc agatatttat agtacaaagg
    29881 tgctgcgcag catgcaggga agcaattatc atttgcctgt gttgcaaaaa gatttgacag
    29941 aagtgatttc tttaatgaaa gacaaagggt ttacagtgta tggcacagaa ttaaatgaag
    30001 aagcacacgc attagacaaa gtagaaaaat cagataactt cgcaataatc atggggaacg
    30061 aaggtcaggg ggtaagtcag gaaatcttgt ctttgactga tcagaacgta tatatcccga
    30121 tgaaaggaaa tgcggaatcg ttgaatgttg gggttgcggc agggattgtt ctttatcatt
    30181 tttctttatg attaaggaac tattaagctt tggctatttt atccaaggcg actagaataa
    30241 tccgaacact ggtggtatac ttagatcaat ttcagaagaa aatgaaattc aaaaaggtgg
    30301 cagatcgata attatgacag aaaaatggcg tgaagacgaa gaatatatgt cttatgtaga
    30361 agatctttta gaaacagaag cagtgaaaaa attagcgaat tacacacagc atgttcattc
    30421 cactcgtttg gagcattcca tcagtgtttc ttactatagt tatctgctcg caaaaaaatg
    30481 gggtggcaat gctaaagcaa cagcgcgtgc tggacttttg catgatctgt tctactatga
    30541 ttggcgtacg acgaaatttg atgaaggcac acatgcgtat atccatccaa gaattgctgt
    30601 aaagaatgca gaaaagatca ccgaattgtc ggatcttgag cgagatatca ttttgaagca
    30661 catgtggggc gcaacgattg caccgcctaa gtacaaagaa ggatatatcg taacattcgt
    30721 agacaaatac tgcgcagtca aagaagccgc tcagcctatg tcagcaagta tgcgtaagag
    30781 atggcaacgt tactttggga aagaatcctc gatttaatag ataaaaccaa taatagacca
    30841 tattgaggtt gtgacagaag tggtcagctt caagaaataa gaaggaattt acgaaaattg
    30901 cttctcaaat ttttgtgaag tttcggctta ttaccgcaga tgccagcttt tgaacaccat
    30961 tcattcgaaa caagggactt tgacacgact tttgtcacag tccttttttc tgtttgcagt
    31021 tgactatctg ttcaagatac ggtttacttt tatttatcga atgaatcatg tacatagaat
    31081 aataagcatt aaagggggtg atgataagat gcaaaacgta tcttatttaa aagagtccgg
    31141 gatcttcagg taacacatac tttgcagaag atcacttttt tctacaaagt atgcactttg
    31201 gttagaagaa aggactttaa aatcatggaa caaacaataa aaccatacca atattttttt
    31261 ataataagag aagtagaaca gttattaaat gcgtactcat ctgtcaatga tccaaaaacg
    31321 gtacaaacag ttcaggcact agcagctgaa aagattcgag acatattaga tcacgaactg
    31381 ccagaatcga tacattttta acgacagttt tagacgtaaa actgacaaaa gcgcaagcag
    31441 aaagagcatt ggaggagctg aagcaaatcg ttcagccgtt tatgcagcct agcaaaccac
    31501 aaatcgagaa attattccga aaagtgaaaa aactcaaaca accagcttgg aatgaaatcg
    31561 acttgcaaga acatacgtac atcggctgga atgatccagg tacgcagaaa aaattcatgg
    31621 tctattacga agatgaaaaa ttaaaaggga ttagcggtac tttatcacca acgataaata
    31681 agggaatctg tgcaatctgt cagaaaacat cgaatgtttc gctttttctt tctacaacga
    31741 aagcaggttc tgatggcacg tatacgaaaa aggggaatta tatttgtcat gatagtgacc
    31801 aatgtaatca acagttaacg aaattggctc ctttctatga atttgctgaa cgtgtaaaga
    31861 aagaacgata attttattgt tttttacaga aaaatatgct atcttttaat ggaagtgaat
    31921 aggtgatggt gttcgccttt aaatgctaac gaaagatagc taatgacgcc tactaaagtc
    31981 tggggacagc ccctttcttt agtaggcgtt tttttattga ggaggaattt tttatggaaa
    32041 aacaattacc gagtccagta cgcattttag tgtatatgct gaagtggctg gtgatcacag
    32101 cgattctcgg tatattcatg gggagcttgt ctgctttttt cttaaatagc ctgacagttg
    32161 tgacagatat ccgactatca catccttggc tcctttgtct actaccagtt agtggtgcgc
    32221 tttttgctta tctctatgcg tatcacggcg gactttctag tcgtggaaac aacttagtga
    32281 tcgatcaagg aaatggtgga gaagaaaaaa tcccgttgcg gcttattcca cttacactgt
    32341 ttggaaccat aactacccat ttatttgggg gatctgttgg ccgtgaagga acagcagtcc
    32401 aaatgggggg agcgttagca gataatacgg cacgtttgtt ccgattggat aaatcagaac
    32461 gggaaatctt gattatcagt ggaatcagtg cgggttttag ttctgtattt ggcacacctt
    32521 tagctggaac attatttgga ttagaagtgt tggcaatcgg gaaagtccga acagaagcga
    32581 tcttccctag cttctttgct gcactgtttg caaatttcat gacagaaagc tatggtgttt
    32641 ctcatctaca ttaccaaatg ggtgtgattc ctgaatggag tatgttgctg ttcttcaaag
    32701 tttttgttgc tgggattgct tttggtttga ttggttgggt attcagccgt tcgatcgtct
    32761 ttttaaaagc gcgatatgca caatggattc ctcatcctgt ttgtcgaaat acgataggtg
    32821 gagctgtagt tgtggcagca gcattgattt tgcaaacaca acgttatctc ggattgagtc
    32881 ttccgttact tcaagaatca tttaatggac aggcacatgt ctatgatttt attgggaagt
    32941 tattcttcac agttttgtct ttaggcgcgg gatatcaagg gggagaagtg acaccattgt
    33001 ttgaaattgg tgcaacgctt ggtgcagcac tagcgccttt gctccatgtt tctgttcctt
    33061 ttcttgcagg attgggcttt atcggagtat tttctggtgc gacaaataca ccgattgcct
    33121 gctttgtgat ggggatcgaa ttgttcggtt cacaagcggc agtatacttc tttatgatct
    33181 gcttgatcag ctttatgtgt tcaggcaaca gcggtattta ttctgctcag caagtagcgg
    33241 tcaaaaaagg gatactcttt ttgccaatag ttaagagaat gaaagaaaaa aacaaggtgt
    33301 gacatatgtt ttgtcacacc ttgttttttt agaaaagtaa agtaatcagc gataagaatg
    33361 cggagcctgc gaaaagcaat ccgcaaatga tcatatgtct ttttttgttc atcatcaaga
    33421 tggcgaggta ttctcttaaa aacgcattag gcaagtagta gaaagctgtt tttgcaccaa
    33481 taccatttgc tttcaatttg gcttgccgcg cataaagacc ggctctaaaa atgtggtaat
    33541 tgttggaact aaagatcgcg cggtatggcc ctttcatttg ctgatccatg atttcctttg
    33601 aatagagcat gttttccaaa gtggttgtcg aattggtttc gacaagaatg tctctttcag
    33661 ggattccttg ttccatggca tattgtttca tcgcaatggc ctctggtact ttttcgtctg
    33721 ctccttgccc gccagacatc aataagatgg gcggatttaa tgtagcacgg ctttgagccc
    33781 gataaaaagc aattgcctta ttgattcgct tggctagaag aggagaaact gtttctccat
    33841 tgatcaatcc agcgcctaac acaacgatga agtcttgatt gtatcgaggc cggttgaact
    33901 gatacaagaa tgagacagtc aaaaagttgt agaatacgat aaatagatag atcaagatca
    33961 ggggaacgca gaaaaacaaa gcatttatcc attgcggcaa atattttaac aagaaaaagt
    34021 caaaaacaag aaacagtgta agaaaaatgg ctagtatcaa cgttaaaaga ttagctaatg
    34081 aatgcgattc tctacgtaaa actgtgaccc cgttccaaaa aagaaaaaca atcaatccat
    34141 aaataccaaa taaaaggaca agtagcaatg gaatcgtgat caaggcgatc agtccgccaa
    34201 gaaaaatatt ttgcgtttca tacagcaaga caaacaagta gatgccgaaa ctgatgagta
    34261 aaacattgaa taaaagacca ttcagtagcc ttcgtttttc tgtgaaataa gaaaaagcaa
    34321 agatactaaa gaacaacaat ggaatcaaaa aataaaaggt cgaataaaaa atttccaaca
    34381 cgacaaaata agttaagatt accgtaaatg tccacgtact gccgtaaaag aacaaggaag
    34441 gatccttctt ctgttttcgc caataaacga tagagaatac cccaataaga tagagaacta
    34501 aaaagatcag atctctggtt agattcataa attcaactcc tttttcttat acagtataac
    34561 gaaaatagat gaaaaaagct gttatttaaa ctagaaaaag caaaaaaaca tctttccgat
    34621 ggaaagatgt ttttcttatt gttcaattaa aacaagttgc cgaaaaattc tgcgacgcgt
    34681 cgccagccaa tcacgaatat attcgctttt tccactgttt tatccgtaat gatctctgtt
    34741 gatggtgcat tgttgtcttc taaatagcct aatttatcgt ctgccaaggt gatcgtagcg
    34801 gtaccgattt ttgtaccttt atccataggt gcttggatct cattgtcagt gacttgcttc
    34861 ttgtcaagtg ttggcgtgat cgttaatttg ccagtatcca tatcgtttcg tacccatact
    34921 tttacagggc ttttaagcac aacaggaact gttgattctt ttccgtcttt tacatcaatg
    34981 gtttttagac taggaatact tgcgttagct tttccgagct ctttttctga ccaattgtca
    35041 tagcagtagt ccatcaaacg gtctgtttca ttgaagcggg cactcgggtt ttccgcatgg
    35101 ccgtccgcat tcaatacgac agtgatgatc cgttgatcgt ttttagtgat cgtaccaacg
    35161 aaacaagcac ctgcaagctc agtcgtacca gtttttaatc cgtccacgcc ttccttgtaa
    35221 ttaacgaatc ctgggagcat ccagttccag ttgaccattt ccactggtga ttgcgtattt
    35281 tcgccaaaca tttgggtcgt agtacttgat acatccagta tttctgggaa atccaagatc
    35341 aaatggcggg caacgatggc cacatcttga gcagacattt gattttcgtc gttttctcca
    35401 gtgccttctg gtcggttttc gccaagataa gagttgttca atcctgatgc attaacgatt
    35461 gttgcatctt tgattcccca atctttcaat tgttctttca ttcgttcagt gaatttagct
    35521 tcgctgccag agattttttc tgccaaggcc accattgaag cattggctga ctggatgatt
    35581 gcagagtcga ataattcttt gacagtgtag gtgttttctt tatgcaaagg aacattcgat
    35641 aaatctgggg tgatactcaa attttctgca taatctgaaa tcgaaacttt atcaccccat
    35701 gacagttttc cttcttttac ttgatcaagg acgatgtaaa gaccgatgat ttttgtgatt
    35761 gaagcgatcc ccatggtttt ttctccatct tgatcataaa gaatcttacc gctttgcgca
    35821 tctacagcaa aagctgcttt tgcatttact ttgaacgtat cttcagcttg cacagcaaca
    35881 gctggtagaa aagaagcaag gcaaagaatg agactagcca ataataggac tagacgtgtt
    35941 ttttttatgg acatatccga tctccttgta attaatttgt ttcgctacaa attaaaattt
    36001 tatcagagag gggagagagt ttcaatgttt ctgaaaaaac tttaggataa gaacgcgcgt
    36061 tatggaagtt tagcggtcgt ttttacgtac ttttacagga ataggctgtg tttccgcatc
    36121 taataagcgg gaagcgctga aagcaaataa actgataata cttaataaac tgaaaatcat
    36181 aatcaaatgc ataagatcca cctcttttat tctttttctt agtttatcag catttcatga
    36241 agaatagttg aagactgggg ctaaataatt gaggaaatta tgaagataac ttaaaaaaat
    36301 agctaaattt aaaaccgtag aaaccgtttt atttttttag atacgtttta ttgtatggaa
    36361 aaatagtgtt gtggtagact ttagatagaa acaaaggaat gattttttta ggaggggtac
    36421 gtttatggca aaaaaggata aagaattatt gacacaattg acttcagaag gcgctaaagg
    36481 accattcgtc acgatcatgc tgaatacgca tgtcgcccat caagatgtgg aaaaagatca
    36541 gatcaaattc aaaaattttg cgaaagaagc aaagaaacgt tttgagaaaa aatattccga
    36601 acatgactgg tccccgttcc aagaaaaaat tgaaaatttg ttaggagatc cttcgttttg
    36661 gcgtagtgca actacgagtg tagcggtcat tataactgct gaccagacct atatccatcg
    36721 tttgagtatc ccggtagacg atcaatacta tgtaagtgat cttccttatt tactggctat
    36781 tatcaaaaat gggcagttca actattctta ttatttattg ggattgaacc gcgactccat
    36841 gaaattgtat gaggtcagca acaaacaagt aaaagaagta tctctaccag aaggggcacc
    36901 ggttgatgtc gttactgcat tgggatctga actgacaggt ggcggtttga acttctcttc
    36961 taatggagga ggcggagccc gagatggagt aactttccat ggcgtaaatc caaaagatga
    37021 agaagtagag atcgattggg tcaactatta ccaagctgtt gatacgtact tgaaggattc
    37081 tttcgaaaat cctgaacaat ggccgattta tttatttgca ttaccagaaa atcagacgat
    37141 gttcaaaaaa attgctaaaa atccctatta ttgtgcagac gttgcgattt ctgcttctcc
    37201 tgcgcagcta tcctttagag atatcgaaga aggggcgaag aagctgtcaa gtgaactcgc
    37261 agctcaagaa tcaaaagaat acaataaact cttagataag aaattcatcg atcagttagt
    37321 tgatatcgtt ccagctgcga aagaaggaaa agtgtcacat ttatttatcg ctacgtcaaa
    37381 cttagctgat ggtttcggtg aagatcctga aacagaatat gatcggcgtc aagtgctgaa
    37441 tgcattagca gatgatgtga tcattaatgg cgggaaagtg tttattttgg atcaaaaaga
    37501 tgcgccagat gaaaagagtc tgacagcgat tcttcgttat taaaagaggt tgtgaaatca
    37561 gtgttttgac ctgagtatta gagaaacaaa cggaaaaata gtttcacata tttttccgtt
    37621 ttttgagcta ataccgaagg tcagctgatt aaacaccgtg tatgagaggt tgtgaaaaag
    37681 ctgttctgca tcaagtaata agaacagcaa gcaaaaatag ctcttcatat tttttgtttg
    37741 gtgggcttat taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgacag
    37801 aagtggtcag cttcaagaaa taagaagaga ttgacaaaaa ttatttctca aatttttgtg
    37861 aaatttcggc ttatttccga agaagctact tctggaacac cgtttaaa
//
LOCUS       DAZKOW010000013        35438 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000013 DAZKOW010000000
VERSION     DAZKOW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.013"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1152
                     /locus_tag="ACG912_000717"
     CDS             <1..1152
                     /locus_tag="ACG912_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFQ0512059.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG912_000718"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACG912_000718"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFQ0512060.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="ACG912_000719"
     CDS             2728..3003
                     /locus_tag="ACG912_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFQ0512061.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="ACG912_000720"
     CDS             3009..3776
                     /locus_tag="ACG912_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFQ0512062.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="ACG912_000721"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="ACG912_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFQ0512063.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="ACG912_000722"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="ACG912_000722"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFQ0512064.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="ACG912_000723"
     CDS             7920..8222
                     /locus_tag="ACG912_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFQ0512065.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="ACG912_000724"
     CDS             8225..9388
                     /locus_tag="ACG912_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0512066.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="ACG912_000725"
     CDS             9414..12842
                     /locus_tag="ACG912_000725"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFQ0512067.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="ACG912_000726"
     CDS             12863..13987
                     /locus_tag="ACG912_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFQ0512068.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13968..14414
                     /locus_tag="ACG912_000727"
     CDS             13968..14414
                     /locus_tag="ACG912_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0512069.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="ACG912_000728"
     CDS             14398..14736
                     /locus_tag="ACG912_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFQ0512070.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG912_000729"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="ACG912_000729"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFQ0512071.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="ACG912_000730"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="ACG912_000730"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFQ0512072.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="ACG912_000731"
     CDS             15893..16936
                     /locus_tag="ACG912_000731"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFQ0512073.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="ACG912_000732"
     CDS             17095..17823
                     /locus_tag="ACG912_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFQ0512074.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="ACG912_000733"
     CDS             17939..18184
                     /locus_tag="ACG912_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0512075.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="ACG912_000734"
     CDS             18181..18399
                     /locus_tag="ACG912_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFQ0512076.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="ACG912_000735"
     CDS             18535..19074
                     /locus_tag="ACG912_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFQ0512077.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="ACG912_000736"
     CDS             19067..21340
                     /locus_tag="ACG912_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFQ0512078.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="ACG912_000737"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="ACG912_000737"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFQ0512079.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG912_000738"
     CDS             complement(22513..22767)
                     /gene="rpsT"
                     /locus_tag="ACG912_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFQ0512080.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="ACG912_000739"
     CDS             complement(23167..23436)
                     /locus_tag="ACG912_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512081.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23698..23826)
                     /locus_tag="ACG912_000740"
     CDS             complement(23698..23826)
                     /locus_tag="ACG912_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512082.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(23906..24361)
                     /locus_tag="ACG912_000741"
     CDS             complement(23906..24361)
                     /locus_tag="ACG912_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFQ0512083.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="ACG912_000742"
     CDS             complement(24499..26427)
                     /locus_tag="ACG912_000742"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0512084.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(26624..27457)
                     /locus_tag="ACG912_000743"
     CDS             complement(26624..27457)
                     /locus_tag="ACG912_000743"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFQ0512085.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27649..28353)
                     /locus_tag="ACG912_000744"
     CDS             complement(27649..28353)
                     /locus_tag="ACG912_000744"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0512086.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28376..29002)
                     /locus_tag="ACG912_000745"
     CDS             complement(28376..29002)
                     /locus_tag="ACG912_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0512087.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29328..31703
                     /locus_tag="ACG912_000746"
     CDS             29328..31703
                     /locus_tag="ACG912_000746"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFQ0512088.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31727..32218)
                     /locus_tag="ACG912_000747"
     CDS             complement(31727..32218)
                     /locus_tag="ACG912_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFQ0512089.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32405..33325
                     /locus_tag="ACG912_000748"
     CDS             32405..33325
                     /locus_tag="ACG912_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0512090.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33479..33823
                     /locus_tag="ACG912_000749"
     CDS             33479..33823
                     /locus_tag="ACG912_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFQ0512091.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(34013..34513)
                     /locus_tag="ACG912_000750"
     CDS             complement(34013..34513)
                     /locus_tag="ACG912_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0512092.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            35041..35124
                     /locus_tag="ACG912_000751"
     tRNA            35041..35124
                     /locus_tag="ACG912_000751"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35075..35077,aa:Leu,seq:cag)
BASE COUNT        11775 a         6170 c         7295 g        10198 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa ataggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagagaagt gttctggttt tttctacatt gcaataaacc
    26641 aataaccaat caaaatgata ccaaggataa tacgatacca gccaaaagcg gtaaagtcgt
    26701 ttttcttcaa atagcttagt aagaatttga tagctaagac ggaaaccaca aaagatacga
    26761 atgttcctac aagaagaaca acagtttctt ccattttgaa tgaattccct tcagctaaat
    26821 atttgacgat cttcaagccg cttgcgccaa acatgacagg aatccctaag aaaaatgaaa
    26881 actctgtagc gacaaaacgc gaagcaccaa tcaagataga tcctaggatc gttgcgccag
    26941 aacgggatgt tccgggaaca agtgccaaga cttggaataa cccaacaaga agagctgctt
    27001 ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt gtttttgttg cgtttttcga
    27061 caataataaa ggcaataccg taaacgatca aaacaatggc aactggtaag aacttgtgga
    27121 aatttgcttc taaccaatca tccaaaggca aaccaatgat gatggctggt atgcaagcaa
    27181 cgatcacttt aaaccaaagt gtccacgtat cttttttttc taccgttgat ttacttggag
    27241 aaaatgggtt taacttgtga aaataaagaa cgacgacagc caagatcgcc cctaattgga
    27301 taacgacatt gaacatcgac ataaattgtg aactggcatc cagtttgatg aattcatctg
    27361 ctaggatcaa atgaccagta ctactgatgg gcagccattc cgttattcct tcaataatac
    27421 cgagaatcac tgctttgata atatttgcaa aaaacataaa ttccttcctt cccaattaag
    27481 atacatctta tagtataccg ttattttttg agaaacccaa tctgtttttt aatattaaga
    27541 aaaacttgag aaataaagca gataagtaca cgaagagaaa aaagaagaca aagccctcgt
    27601 tcagactttg ccttttatga ttctctggta aaaattaaaa tatccccatt atagaaacga
    27661 gtcgcgacta tgtgttttcc cgttatcgat ataagacgta tcattgatgc cgtggataga
    27721 aaatcgaccg tttgcataag tgacctttgt tacgctgcca tttaaaagcc cagcacgaac
    27781 aggctgttta ggatcgatca gattaagcaa aaaggcgatc gtcaaaccat gagatacgac
    27841 taaaacattt ccaccaccat ttttttcgca gtgatgggcg atatcttcaa atcctgtcca
    27901 tacacggtct cgcaatgttt cataagtttc cgcccaccca gcggtatcta agttgtatac
    27961 agcattagca atttgttcaa aagaaacttc tgtggtaaac atttcatcat atgttttaaa
    28021 attcaataca cgagggatca caccccacat ttccatgtca tatccacctt caagagaccc
    28081 gaaacaccat tctctaatcc ttttttcaat aaaataagga atttcttttc ccttagaatg
    28141 ttctcctaga ataatacggg cagtttcgat tgcacgacca ctgtcactag aataagcaaa
    28201 tgaaaagtcg acatttttta acccgcgacc taaataatga atgccttctt gtccttgctt
    28261 tgtgaggggt gtatcacacc agccttgtac tcgttcaatt gtattgaaca tcgtttttcc
    28321 gtgtcgtacg atataaagtg tagtttctgt cattttattc tcctcctaag tcgttttaac
    28381 gattgaaaaa aggatttgtc cttttttcct gttcaatcgt cgttgcatcg ccatgtcctg
    28441 gatagactgc aaattcatca ggtaatacaa acagctgggt ccgaatacta tgaagtaatt
    28501 gttctaaatt ccctgtatac agatcggttc taccgatact tcctctaaac agggcgtcgc
    28561 cagaaacgac aaaatcatca aaaataaagc tgacgcttcc tatagaatgc cctggggtag
    28621 ggacgacgcg aaaagacatg tttcctaaag tataagtttt catttcaaat tcatattctg
    28681 caggcgagac aataacatca gcaatatcat catgtctacc gagtccagaa agattcaaaa
    28741 tcggatcacc tagccatttt tgctctagtg ggctgacata aactgggatt tgataatacg
    28801 cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt atgggtaagc aaaatcgcta
    28861 ccggctgttg gtttgttttt ttgatttctt gtttgattcg ttccgcatct tctccagggt
    28921 caatgatcaa aagtgcttct tcgttgtaga ccaaataaca gttttcttca atcattcctg
    28981 tttttaatct ttcgatatgc atgtgcattc ctccttgata gccaaaactt ctttcatcca
    29041 ttagtataac gcattcattg ctaggaaaca aaaacaggta cttatttctc tttacttatc
    29101 tgaattatca gactatatga aaagtatttg gatattctaa agagaaggaa agtaagtttt
    29161 catagcattt taatatgtcc ttgttacatt agattcatcc aaaaatccaa attctatatc
    29221 ctatatccct gcctaatcag cagggtgttt ttttatgttt ggaaagagag ctctttttgc
    29281 taaactaggt tttttcaggt aatcttagag agaaagaggt ggtggaaatg atcaaacagt
    29341 caaaaatatc tgtgcggcac ttagtagaat ttattttgcg acgtggaagt atcgacaatc
    29401 gtaaaaaaag taaccatact gcattagaag gagcgagaat ccaccgcaag ctccaaaaag
    29461 atgcgggaga aaactatgaa aaagaagtct ttttaaaaac aacagttcaa ttagatgaat
    29521 attacctgat cgttgaaggc cgtgcagacg gtattttcaa aagagacggt atttattata
    29581 tagatgaaat caaaacctcc gaaccacgat ttgaagattt agaaccagaa caagtggaac
    29641 ttttctttca tcaagcaagg gtgtatgcct atatctatag tcaagaacat gatttacaag
    29701 aaataaatct gcaattgact tacttccaga cggcagaaga agtgatcacg agaaaggttg
    29761 aacatcagac aagagagcag ttagaaactt tttttaaaaa gctgactgaa gattataaag
    29821 aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa tgcctctttg atggctttga
    29881 aattccccca tgatgagtat aggaaaggtc agcgtgaact agcggtagct gcttataaaa
    29941 cgatccgtac gaaacagaaa ttgtttgtag aagcacctac cggaacagga aaaacaatct
    30001 ctacgttatt ccccgctttg aaagctgtag gggaagaaga gggagaaaag atcttttatc
    30061 taacggctaa aacaataact agacaagtag ctgaagatgc tatgacagca ttaaaagata
    30121 caggagctga agtaaaaagt gtcactttga cggcgaaaga caagatctgc tttttagatg
    30181 agacgacctg taatccagat cagtgcccat atgccaatgg ttattacaat cggattaatg
    30241 aaggtttatg ggatcttttg aatcatgaga atcagatcac tcgtgaagtc atcgaaactt
    30301 atgcaagaaa gcatacactt tgtcctttcg aactatcatt agatgtcagt atctggtgtg
    30361 acgtaatcat tggggattac aattatttat ttgatccaac tgtttattta agacgttttt
    30421 ttgaagatga aaaaaacgag gactatctgt ttttgattga cgaagcacat aatttggtga
    30481 atcgttcccg agagatgtat tcggcagaac tatcttatga aaaaacgaaa cgaagtaagg
    30541 aagctatacc aaaggaattc aaaaaacttc atcgccgttt caacaaacta ttaaaagaat
    30601 ttgattcgat ccgagaaata gcaaaagaag atcattggga ttatcatcat caaaaagcac
    30661 cggctgaatc tttagtaaag gcaggttatc aattaagcga aaaaattaaa gaatggctgg
    30721 cagaatttcc tgagcaccca tcacaagaac aattgcttcc ttattatttt gatttgcttc
    30781 attttttgaa ggtcagtgaa ttttatgatg atcactacga aacaacagtc gaaaaaacgt
    30841 atcgcgatct gatagtaaaa gagttttgta ttgatccaag cttgtttctt gagcagtcgt
    30901 tggataaagg aaaaagcagt attctttttt ctgctagttt ttcaccactc tcttattatc
    30961 aggaaacact ggggggacaa aaaagtctgg cttacaaact gccgagtcct ttcccagaag
    31021 gaaaccagca agttttcatc gcaaattacc tagagacgac ttatcgaaaa agggaacaca
    31081 gtctggcaaa attagtagag acgattcatg cttttgctga tggaaaggtc gggaattatt
    31141 tcgttttctt tccttcttat caatatttag atcttgcggt ggaagcattc agacagcaat
    31201 atcccgacag caatgtgcta atccaagaaa cggacatgaa tgaggaagaa cgcgaacgat
    31261 ttttaacaaa atttcaaaca aatcccgctg aaacattgat tggattttgc gtacttggcg
    31321 ggattttttc agaaggaatc gatttgagag gggaccgatt gatcggcagt atgatcgtag
    31381 gtgtaggttt gccgcagatg aatcatgagc aggaactgat caaatcatat tatgatgaga
    31441 aggaacacct tggatttgct tacgcttatc agcttcctgg aatgaacaaa gtcttgcaag
    31501 ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat cgttgtattg gcagatcaac
    31561 gattttctag tttttcttat cgacgtttat tcccacaaca ttggcaaaca gcaagagaag
    31621 tacgaagtgt tggggaattg gaagaaggaa tacagcgttt ttggttatca aaagaagcag
    31681 taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt ttttgattaa cgatagttag
    31741 tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa agcaatcaca gcttgtagat
    31801 catcacggtt cttacctgtc actctaatct gatcttcttg gatttgtgct tttactttca
    31861 gtttttcatt tttgatagct gtagtgattt tcttcgcgtt ttcacgatcg attccgttga
    31921 ccaaatcggc agtttgtcga gctttgccac ctagagcgtg ttctgattcg gaaaaatgga
    31981 tatttttgat tggcacattt cttttgatca gcttaccaaa taatacatct ttgatctgtt
    32041 cgattttgaa atcatcatcg ctcaatacaa ctagattgct gttctctaat ttgatttcta
    32101 ctgtagaatc cttgaagtca aagcgattgg acaattcttt tgttgtttgc tgaatcgcat
    32161 tttttacttc ttctgtattg acttctgata caatgtcgaa gctagcatct tttgccatag
    32221 gaaccctcct gatatacata taaatattta ccatacatct atcttatcat aggattaggg
    32281 cagattctta cctgtttgct aaaattcttc tcatttgagt tcgaagtatt ttattttaat
    32341 agataaactg ttattcttac atttggaata ccaaagtagg ggagaggtgc ttgtgagtaa
    32401 aattatgact gcgtatcata aaaatgaaag attaaagaaa atagtggtta ttttgataac
    32461 cggattgacc gctgcggtcg ctttaaatta ttttttgatt ccagccaatg tcttttctgc
    32521 agggatgaat gggatcgctc aaatcatttc ctcgctttta tatgaatggt ttcatatcaa
    32581 gatagacaca ggaacattta tttttctttt gaatatccct gtgtttgtat tagggtttct
    32641 caaagtagga aaacgagcaa cgatccttag ttttgtgaat gttgtatgtg tatcggtgat
    32701 gacgatgctg cttccagtcg gacagatcac tgataatatt ttgatgaatg ccctagtagg
    32761 cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa atgggcttta caactggtgg
    32821 gatggatatc atctctcttg tactttcaca aacaactgga aaaacagtcg gaaactacat
    32881 gctgattcta aatggtatca ttgtagtcat cgcaggcttc ttctttaact gggaaagtgc
    32941 cttgtatacg attatttcca tttatgcaat gactcaagta attgatgcca tccacacgag
    33001 tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca gaagaggtaa cagaagaact
    33061 agcaaaacgg atggtacgtg gaatgacatt actgccgtct gtcggtggct attcaaaaaa
    33121 acaagggaac atgatcatga tggtcatcac ccgctacgaa atgtatgatc ttgatcagat
    33181 cgttcatgaa atcgacgacg atgcatttat caatatttta ccaactcatt cagtatttgg
    33241 ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca actggcgtat tgccagaaat
    33301 aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt ttagaataaa tttaatctat
    33361 tacatctaat aagaacattt ttataaattt aaacgagaat cttacttcta aattctcatt
    33421 tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt gttaaggagt gtcgtacgat
    33481 gagcgaagca aaccaagaac ggtctggaca agaaatagaa ctaatcaaag aacgtattct
    33541 tgctgccttg gaaatggtga ttgatcctga attaggaatc gacatcgtca atttaggctt
    33601 gatttatgat atagaattca atcctgaaaa tggagaaaca gtcattaaga tgactctaac
    33661 aactatgggc tgtccattag cagatatttt aacagaatct atccatgacg cattaaaaga
    33721 agtgccagaa gtaacgaaag cagaagtcaa actagtctgg tatccagcat ggacgacaga
    33781 taagatgagc cgttatgcac ggattgcgtt ggggatacga tagaaatctt ggctgactag
    33841 ctatgagaag caaataggaa aaacaaaaaa gccttttatc ctttctagaa gaaaagagat
    33901 aaaaggtttc tttgtttata aaattttgtc tgctatatta taagttaatt aaataaatgt
    33961 gttctattga taaaggacat tcaaatccct ttccttttgg aatccttctt ttttacatca
    34021 gtttgatttt cgtatagtat tctttctcat cttcacgata aatcgcagcc ccattttttt
    34081 gttgtgcttt aagactgcca agattttttt tatttacaga caaaagtgcc tcatcaatgc
    34141 cgattttttt tgcctcttgt agtactaatg ctaatccttg tgtggcatat ccttttttac
    34201 gataattagg ggagatgccg taaccaatat ggccagcgcc gtttcttaat gtttcgttca
    34261 gtttatggcg caaattaaaa attcctacat actcatgttc atcactttct aaaataaaga
    34321 cggttgcggg aacgtaactc tcaggaagat cgattcctaa agacgttttt tcgcattttt
    34381 ttacataatc taaaaattca gcaaatgtat agccataagc attgttgata aatccactct
    34441 catttggtcc aaagctagtt tgtagctgat aagctttctt taaatctgct tcccatagtt
    34501 tgatcagttt catcgtattc acctctttaa gtaaccttat tcatttgttt tctactatag
    34561 cactattgtt gtaaatagcc atgcctattt ttatttattg tgtcatatcg tattaagaat
    34621 catcgaacaa tacaaatatg aattacaata aaaaaattat ttgaagttcg ttcattcgtt
    34681 acttatgatc tctagaagaa agaaaacgtg aattacttca gcgtctagaa ttccagaagg
    34741 atagtaatca acagaagaaa taatcaatat ccaaatgaaa gaagatagct gaatcgagaa
    34801 aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta ctaatcccag agtcgttcgt
    34861 ttaacattga aatcgatcat atggataaaa cagatttttt caccaaccct caatagatga
    34921 tggagttgct cggatgaatt atttgtgtag atggcataag ggagggcgta cagaaatgaa
    34981 aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat cctaaactaa aagatgtgat
    35041 gcggctatgg cggaattggc agacgcgcta gcttcaggcg ctagtgaggg tgacctcgtg
    35101 gaagttcgag tcttcttagc cgcataattt gtttttttat aaagagaagg tacacctgga
    35161 gcgatgagaa agaaaataac atattatcta aaagaatata ggaaaaattt taaacaacgg
    35221 aaccagattc aaataaatca aaaaaaattt gtgtgaaggt gggacagttt aaaacctaat
    35281 agattgattc aaatagtaga tttatagatt ctgatacaag aaatactggc aaacgatagt
    35341 taaggaataa agactaaact taaactcaaa aagaggtcat gcgaaagaat aaatcgcatg
    35401 acctcttact atattttgaa tgtgtttaaa cgttcaaa
//
LOCUS       DAZKOW010000014        34962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000014 DAZKOW010000000
VERSION     DAZKOW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.014"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACG912_000752"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACG912_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFQ0512093.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACG912_000753"
     CDS             complement(2020..2922)
                     /locus_tag="ACG912_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0512094.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACG912_000754"
     CDS             complement(2919..3611)
                     /locus_tag="ACG912_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFQ0512095.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACG912_000755"
     CDS             complement(3654..4295)
                     /locus_tag="ACG912_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFQ0512096.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACG912_000756"
     CDS             complement(4333..4647)
                     /locus_tag="ACG912_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512097.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG912_000757"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACG912_000757"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFQ0512098.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG912_000758"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACG912_000758"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFQ0512099.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACG912_000759"
     CDS             complement(5984..6916)
                     /locus_tag="ACG912_000759"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFQ0512100.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACG912_000760"
     CDS             complement(6913..7689)
                     /locus_tag="ACG912_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFQ0512101.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG912_000761"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACG912_000761"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFQ0512102.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACG912_000762"
     CDS             complement(10933..12009)
                     /locus_tag="ACG912_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFQ0512103.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACG912_000763"
     CDS             complement(12014..13294)
                     /locus_tag="ACG912_000763"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFQ0512104.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACG912_000764"
     CDS             complement(13313..14239)
                     /locus_tag="ACG912_000764"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFQ0512105.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACG912_000765"
     CDS             complement(14252..15535)
                     /locus_tag="ACG912_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFQ0512106.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG912_000766"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACG912_000766"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFQ0512107.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACG912_000767"
     CDS             complement(16323..17234)
                     /locus_tag="ACG912_000767"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0512108.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG912_000768"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACG912_000768"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFQ0512109.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACG912_000769"
     CDS             complement(17700..18398)
                     /locus_tag="ACG912_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFQ0512110.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACG912_000770"
     CDS             complement(18385..18771)
                     /locus_tag="ACG912_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512111.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACG912_000771"
     CDS             complement(18918..20588)
                     /locus_tag="ACG912_000771"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFQ0512112.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACG912_000772"
     CDS             20771..21004
                     /locus_tag="ACG912_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0512113.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACG912_000773"
     CDS             complement(21103..21534)
                     /locus_tag="ACG912_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFQ0512114.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACG912_000774"
     CDS             21613..22026
                     /locus_tag="ACG912_000774"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFQ0512115.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACG912_000775"
     CDS             complement(22064..23212)
                     /locus_tag="ACG912_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0512116.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACG912_000776"
     CDS             complement(23334..23930)
                     /locus_tag="ACG912_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0512117.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG912_000777"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACG912_000777"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFQ0512118.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACG912_000778"
     CDS             27160..27591
                     /locus_tag="ACG912_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0512119.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACG912_000779"
     CDS             complement(27636..28985)
                     /locus_tag="ACG912_000779"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0512120.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACG912_000780"
     CDS             complement(29055..30218)
                     /locus_tag="ACG912_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0512121.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACG912_000781"
     CDS             30347..30670
                     /locus_tag="ACG912_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512122.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACG912_000782"
     CDS             complement(30853..32202)
                     /locus_tag="ACG912_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0512123.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACG912_000783"
     CDS             complement(32271..33224)
                     /locus_tag="ACG912_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFQ0512124.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACG912_000784"
     CDS             complement(33326..34516)
                     /locus_tag="ACG912_000784"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFQ0512125.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34962)
                     /locus_tag="ACG912_000785"
     CDS             complement(34716..>34962)
                     /locus_tag="ACG912_000785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512126.1"
                     /translation="IRKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYV
                     FLLVYAINKLNIQNTIDFNERASINGCVVFICPGRVK"
BASE COUNT         9952 a         7642 c         6120 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DAZKOW010000015        34589 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000015 DAZKOW010000000
VERSION     DAZKOW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.015"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..881)
                     /locus_tag="ACG912_000786"
     CDS             complement(33..881)
                     /locus_tag="ACG912_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0512127.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1105..3045)
                     /gene="thrS"
                     /locus_tag="ACG912_000787"
     CDS             complement(1105..3045)
                     /gene="thrS"
                     /locus_tag="ACG912_000787"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFQ0512128.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3382..3561)
                     /locus_tag="ACG912_000788"
     CDS             complement(3382..3561)
                     /locus_tag="ACG912_000788"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFQ0512129.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3608..5431)
                     /locus_tag="ACG912_000789"
     CDS             complement(3608..5431)
                     /locus_tag="ACG912_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0512130.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5709..7988
                     /gene="helD"
                     /locus_tag="ACG912_000790"
     CDS             5709..7988
                     /gene="helD"
                     /locus_tag="ACG912_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFQ0512131.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8081..8728)
                     /locus_tag="ACG912_000791"
     CDS             complement(8081..8728)
                     /locus_tag="ACG912_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512132.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8907..9626)
                     /locus_tag="ACG912_000792"
     CDS             complement(8907..9626)
                     /locus_tag="ACG912_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0512133.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9757..10908)
                     /locus_tag="ACG912_000793"
     CDS             complement(9757..10908)
                     /locus_tag="ACG912_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFQ0512134.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11125..11865)
                     /locus_tag="ACG912_000794"
     CDS             complement(11125..11865)
                     /locus_tag="ACG912_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0512135.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11869..12192)
                     /gene="rsfS"
                     /locus_tag="ACG912_000795"
     CDS             complement(11869..12192)
                     /gene="rsfS"
                     /locus_tag="ACG912_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFQ0512136.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12249..12842)
                     /gene="yqeK"
                     /locus_tag="ACG912_000796"
     CDS             complement(12249..12842)
                     /gene="yqeK"
                     /locus_tag="ACG912_000796"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFQ0512137.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12826..13476)
                     /locus_tag="ACG912_000797"
     CDS             complement(12826..13476)
                     /locus_tag="ACG912_000797"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFQ0512138.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13486..13797)
                     /gene="yhbY"
                     /locus_tag="ACG912_000798"
     CDS             complement(13486..13797)
                     /gene="yhbY"
                     /locus_tag="ACG912_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFQ0512139.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13813..14922)
                     /gene="yqeH"
                     /locus_tag="ACG912_000799"
     CDS             complement(13813..14922)
                     /gene="yqeH"
                     /locus_tag="ACG912_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFQ0512140.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14919..15446)
                     /locus_tag="ACG912_000800"
     CDS             complement(14919..15446)
                     /locus_tag="ACG912_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFQ0512141.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15626..16411)
                     /locus_tag="ACG912_000801"
     CDS             complement(15626..16411)
                     /locus_tag="ACG912_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFQ0512142.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16404..17273)
                     /gene="accD"
                     /locus_tag="ACG912_000802"
     CDS             complement(16404..17273)
                     /gene="accD"
                     /locus_tag="ACG912_000802"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFQ0512143.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17274..18653)
                     /locus_tag="ACG912_000803"
     CDS             complement(17274..18653)
                     /locus_tag="ACG912_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFQ0512144.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18661..19080)
                     /gene="fabZ"
                     /locus_tag="ACG912_000804"
     CDS             complement(18661..19080)
                     /gene="fabZ"
                     /locus_tag="ACG912_000804"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFQ0512145.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19105..19581)
                     /gene="accB"
                     /locus_tag="ACG912_000805"
     CDS             complement(19105..19581)
                     /gene="accB"
                     /locus_tag="ACG912_000805"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFQ0512146.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19584..20819)
                     /gene="fabF"
                     /locus_tag="ACG912_000806"
     CDS             complement(19584..20819)
                     /gene="fabF"
                     /locus_tag="ACG912_000806"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFQ0512147.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20838..21575)
                     /gene="fabG"
                     /locus_tag="ACG912_000807"
     CDS             complement(20838..21575)
                     /gene="fabG"
                     /locus_tag="ACG912_000807"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFQ0512148.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21587..22504)
                     /gene="fabD"
                     /locus_tag="ACG912_000808"
     CDS             complement(21587..22504)
                     /gene="fabD"
                     /locus_tag="ACG912_000808"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0512149.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22611..22835)
                     /locus_tag="ACG912_000809"
     CDS             complement(22611..22835)
                     /locus_tag="ACG912_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0512150.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22888..23850)
                     /locus_tag="ACG912_000810"
     CDS             complement(22888..23850)
                     /locus_tag="ACG912_000810"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFQ0512151.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23860..24300)
                     /locus_tag="ACG912_000811"
     CDS             complement(23860..24300)
                     /locus_tag="ACG912_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0512152.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24606..25571
                     /locus_tag="ACG912_000812"
     CDS             24606..25571
                     /locus_tag="ACG912_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0512153.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25628..25858)
                     /locus_tag="ACG912_000813"
     CDS             complement(25628..25858)
                     /locus_tag="ACG912_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFQ0512154.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25944..26831)
                     /locus_tag="ACG912_000814"
     CDS             complement(25944..26831)
                     /locus_tag="ACG912_000814"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0512155.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26920..28143)
                     /locus_tag="ACG912_000815"
     CDS             complement(26920..28143)
                     /locus_tag="ACG912_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0512156.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28160..29206)
                     /locus_tag="ACG912_000816"
     CDS             complement(28160..29206)
                     /locus_tag="ACG912_000816"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0512157.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29370..31097)
                     /gene="ptsP"
                     /locus_tag="ACG912_000817"
     CDS             complement(29370..31097)
                     /gene="ptsP"
                     /locus_tag="ACG912_000817"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFQ0512158.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31097..31363)
                     /locus_tag="ACG912_000818"
     CDS             complement(31097..31363)
                     /locus_tag="ACG912_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFQ0512159.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31842..34076
                     /locus_tag="ACG912_000819"
     CDS             31842..34076
                     /locus_tag="ACG912_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0512160.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34224..34544
                     /locus_tag="ACG912_000820"
     CDS             34224..34544
                     /locus_tag="ACG912_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0512161.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9771 a         7484 c         6038 g        11296 t
ORIGIN      
        1 ctaaaaaact tatataaaaa atagtaaggt ttttatttcg aatccataaa atattcttgg
       61 attgcttctc gtgttttttt cactttttcg ctaagctttt cgtttttagc catatagaaa
      121 taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg aattagttgc cgcacttcga
      181 ttcgtgattt cttttggtct agatgtttgt acgagtacat cggctatctt gctaagcggg
      241 ttatttccaa attgcgtcaa cgcaatgatt tcgatattca tgcttttcgc cagctcggct
      301 aatgcgacta catctgcact tctacctgag tttgaaacaa gccaaagcac tttctttttc
      361 tcattgctga caagttgcgg gagcaagacg tggatatctt tttcaaaaat gattggctta
      421 ccgacacgag accatttttg cagaatatct tctactacta aagaagaggc gccaacccca
      481 tataaaaaaa cagtctctgc cgattccaaa cactctacga ctttttggat cgtttcttct
      541 tctagaatat ctactgtttc ttttatcgct acttgtgcat tgttaccaag tttgtttttg
      601 atagagtaaa aggactcatt tgcttctata tcactaaaac cttcatcgat tccagtctgt
      661 agttctgttg aaatcgtgat tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa
      721 aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg ctaattcatt agctgtcatt
      781 gtagggacct tctcaatatt accgataatg tagttggcca actttttttc tgctgaagaa
      841 taatcttcca tcatactttc aatacgacta attacgctca taagttcctc caccctttta
      901 atgatagtct atgtaagtat accatatcag acccttattc acggtcgtat actggataaa
      961 aaacaaaaaa acaatccgcc caacagaata tcttatccca tcaatcccac agattctatt
     1021 gagcatctgt gtctttgagg aatacctgtt atctattgtt cggattgctt tctatctact
     1081 acttttttac aggaaattta cctattagtt ttctctgctg taatttttga cttctgcttc
     1141 catagcatca ataaagatat tcaaatcttc tacagctgtt tctttacttc cataacgacg
     1201 tacgttcact gttgcatctt ccaattcctt gtctccaaca actaattggt aagggatctt
     1261 ttgtgtttga gaggcacgta tcttatagcc cattttttca ttacgatcat caacttctac
     1321 acgcaacccc ttcatttgca aacgttcttt gatttcataa gcataatcgc catgtgcatc
     1381 aacagaaaca gggatgattg ttgcttgtat aggtgccaac catgttggaa aagcgccttt
     1441 gtatacttct gttaaataag cgacaaaacg ttccattgta gaaacaattc cgcggtggat
     1501 aactactgga cgatgcgtat tttccccgtc ttctccaaca tatgtcaaat caaagcgctc
     1561 tggcaataag aagtctaatt gtatagttga taatgtttct tctgtaccaa gagctgtttt
     1621 gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc
     1681 tagttctaat tcatccatag cggctttcag cattgcttgg gctttttccc acattgcgtc
     1741 atcatcaaaa tatttatctg tattgtttgg atcgcgatag ctcaagcgga aacgataatc
     1801 tgtaatattg aagtcagcat atactgccac catcaattca agcgtacgtt taaattcatc
     1861 tttgatttga tcaggacgaa caaatgtatg accatcattc aacgtcattt ctcgtacacg
     1921 ttgtaaacct gacaaagcac ctgatttttc atagcgatgc atcattccta attcagcgat
     1981 acgaattggt aattcacgat aagaatggat cgtgtttttg tagaccatca tgtgatgagg
     2041 gcagttcatt gggcgaagaa caagcatttc gccatctccc atatccattg gcgggaacat
     2101 gtcttcttga taatgatccc agtgtccaga tgttttgtac aactcaacat ctcccattat
     2161 tggtgtatat acatgttggt aacctagact aacttctttg tctacgatat aacgttcgat
     2221 cgtgcgtcgg attgttgccc cttttggaag ccagaatggc aaaccagaac ctacttctgg
     2281 ggaaaccatg aacaagtcaa gttcttttcc tagtttacga tggtctcttt cttttgcttc
     2341 ttcacggaga cggatatatt ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac
     2401 acgttgcatc atttgattgt ttgagtttcc acgccaataa gcacctgcca ctgataataa
     2461 tttaaatact tgtatacggc cagtggatgg aacatgtggt ccacgacaca aatcgatgaa
     2521 ttcgccttgt tgataagcag taatcacttc ttcttctggc aattcactaa tcagttctac
     2581 tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc
     2641 gatcggcaga ttttctttga cgattttcat catttctgct tcgattgccg gcaaatcttc
     2701 tgcagttact tggttttgtc cattatctgt atcgtagtaa aaccctgaat cgatagctgg
     2761 tccaacgcca aagtggatat ttggatagag gcgacgcatg gcttgggcca tcaaatgagc
     2821 tgctgaatga cggactaagc caagtgcatc ttcatggtct ggtgtgatga tctcaatact
     2881 tgcatcttcc tcgattggac gagtcaaatc aacgagtttt ccgttcactt taccagctaa
     2941 agcttttttt gctaagcttt ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga
     3001 ctcaaattct ttgactgcac catctggaaa agtaattttt atcataatag tttcctcctt
     3061 gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg
     3121 aaaagagata ctaggacgaa aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt
     3181 cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca agggtttcga aagtggtcat
     3241 cctttggttt tctcctggaa aagtttcagc ctagcttttc ctctcttatc gaagactctc
     3301 aaattcagtt ggctttctca tcaatcaatt atgtgtttat ttaaccacta ataaatagaa
     3361 aaatcaagtg ggaaactaaa cttatttttt ccattcccca tttacaccgt atgttccttt
     3421 tttcatctca ttgatgatga catggatgtg ttcttttggt gcctgcgtgt ttttatgaac
     3481 agcttccgtg atatctttca tcatattttc taattgttcc ggactgcgac cttctactaa
     3541 ttcgacatgt acgaatggca taatgctctc tcctctttct gatcgttctt ttttcaactc
     3601 gattctatca tactatgttt gtcttgggta ctgaatcagg aaaaatggct ggatcttttt
     3661 ttgctctcaa ggcactcaaa atagagaccg tatccacgac ttcttgcaaa acagctccga
     3721 ataatgcggg gattagtccc gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc
     3781 caatcagtac agactgtttc gctattctca tcgtatctct tgaaatcact actgcttttg
     3841 ctacacgcaa gagatcatct ttcaagatga ccgcatctgc actttcacta gcagctgtag
     3901 aaccatgagc ccccattgca ataccgatat ctgcagcagc aagtgctggt gcgtcattca
     3961 cgccgtctcc taccatcgca acaggtcttt gatctttagg gatactttct aaaatggtca
     4021 gtttatcttg aggcaagcta cgggcatgta cagaatctaa ttggactttt tctgcaatcg
     4081 tttgagcaat ggcagatcca tccccggtga tcatcatcaa atttttcaca tcaagttttc
     4141 ttagctcctt gattgtcttt gctgcttctg ggcgaatctg atccgaaaat aggatcgttc
     4201 ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt ggacgtttct atttcttcag
     4261 ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt attgactact gcttggattc
     4321 cttgaccggt cacttctttc agttgactaa caggatacag ttggctgttt ttcacgttgt
     4381 tcactaacga acgagctaag atatggctag attcctgttc tgcactagct gcatacagaa
     4441 gtagctcttc tttttttatt ccattgactg gctgaatcgc ctctacttcc aatatccctt
     4501 tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg
     4561 ttgttccagt ttttacaacg atcccgtttt tacttgaacg gctcattcct gcaactaaag
     4621 caatgggcgc agctagaatc agtggacaag gtgatgccac taccagtacc tcagcaaatc
     4681 gtaaagggtc ttttgagaac caccaagaaa taccaccgat caaataagca atcaacgtga
     4741 aagggactgc ataacgatca gctaaacgga caaatcgggc aggcttttct tttgattctt
     4801 cgactagttt aataatcatt tgatactggc tgtctgcggc acgtttttca gcgatcattt
     4861 ggatcgatcc gtctccattt aaagaccctg acataatctg atctccaggt ttcttggttt
     4921 ccgggcggga ctcacctgtc aaagaagatt catcgaccga agattctcct ttggtcactc
     4981 gaccatctgc gggtacaagt tcgccaggct tgactagtaa ctggtcatca atatttattt
     5041 catcgattac tatatcctgc aattttcctt ctttgaatcg atgggcaatt cttggggtgt
     5101 tatctaacaa agactgcagt tctttacccg ctcgtttatt cgcgtaatcc tctagactat
     5161 ctccgcccgt aagcatgatc aatatcatca aactagccca gtattctttg acggctaatg
     5221 ttgcaaggat tgcggtaatc gctaatacat ctacgccata tcttcctgaa cgtaacgtct
     5281 tgatcatttc caaaaacatc aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa
     5341 atgcaagggc cggctggtga aaaacaaatt cacttaataa agctatccca ccaataagca
     5401 aagtaagtaa aaattttttg aaatgtgtca ttgttttttc tcctctttta tgtgacttag
     5461 agaggttagt aataaatttc cagctgcctc tttcatctta agcatagtcc ttgactagca
     5521 ctttttaaaa caataggcct taattgaaaa caactatcac tatcagcgtt tccaaaattc
     5581 atcagttcta caaacaaaaa tattcagcaa ggattacttt ttcgattcat cagttaaaaa
     5641 gtaccttttt tcgaaaaact atgctatact tttaattcgt tatttacggc ggaaggagca
     5701 aacatgcgat gaatgagaaa caactagaac aaacgcacgt ggatgaaacg atcgaactaa
     5761 tccagatcga acaaaatatg ctggatacaa aacaacaaga attgactcag gaaatgaatc
     5821 attcgacaaa agacacagct aatcacaaga ttcgagctgg ttccaatgaa gctttttatg
     5881 aatctgctgt tgaatatcgc caacatgagc aggaattgct tttgaaatac catactcttg
     5941 aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa agatagtcct tattttgccc
     6001 gtatcgactt taaagaagac cgtgaaacag aaacattata tctaggtatc gcttctttaa
     6061 gagatctggc agaagaaaca atcgttattg actggcgcgc accaattgcc aatctttatt
     6121 atgaaggtga gattggtccc gcattttatg aaactgaaac agatagaata gatgtcgatc
     6181 tgcagttgaa acgacaattt aaaatccaag aaggacgtat tttatctatg gtagatacgt
     6241 cggaagtaat caatgatgac tttttgctag agatattgga tgaatcatca agcaaccata
     6301 tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc aatcattcgg gacacaacaa
     6361 gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac
     6421 gtattgcttt tctgttgtat cataatcgca agtggctcga ggctgaaaac gttcttctat
     6481 tttcacccaa ccatttgttt tctgattaca tctcgactgt attgccttca ttaggtgaaa
     6541 gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca gctgctgcca gagttttctt
     6601 tactagatga acagcaacaa gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag
     6661 tgatgaaaag cggattgacc ttagtcgatc agattgatcg ctatatccaa ggcatcacaa
     6721 actacggtcc cttgttccgt gatatgaaaa taaatggccg tacgatccta tcaaaggaat
     6781 cgattcgcca atggtataaa gaaacaaatg aacaattacc tttacatcat agactttctc
     6841 tattacaaac gaagctcctg aaaaaattag gcggtctgca aaaagacgaa acacgtcagc
     6901 aatgggtcaa agatctggcg gaagaacagc tccaagaact atacgcaaca gatccaaatc
     6961 ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa actggccaac caaatcgtac
     7021 gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta tcaatttgtc aatataacga
     7081 aacaatatct ccactttcta caaactgtgc cactttctct tctaacacga cataaaatca
     7141 gcgagaccag gtggcaggaa catttgacaa ctgttcgtac acacttgcgt caaagagaat
     7201 tgcgtcaaga ggatgctgta ctattctttt tactgatgcg ccgaacatat ccagtgacgg
     7261 ttacacacaa agctcgctat atctttattg atgaaatgca ggatttcgca ccatcacaag
     7321 tggcattgct gcaagccttg ttcccaaaag caaatttgac tttctgtgga gatctaaatc
     7381 aaaatgtctt tggaaatgaa acgatcgttg gatcattgga agaccttttc gtggaccgtg
     7441 aagtcactcg tttccaatta acgacaagct atcgctccac aaaagaaatc actgattttg
     7501 ctaatcattt tattgtgaaa gaacatcaag tagaaacaac agcaagaaaa ggagaccttc
     7561 ctttgctcat ccaaggaaca acagatgata tcaaattgaa ttggttagca gataccattc
     7621 gaacgactca gggaaaagcc cggtattggc gtacagcaat catagctaaa acacaagaag
     7681 aatgcgaagc tctttatgct caactgccag acgagttgaa acaacaagtc cagctgattt
     7741 taaatgagag tgactttatg aagcgttcca ttattttact tccagccttt ttagcaaaag
     7801 gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc agaacatttt tcttctcagc
     7861 aagacaaatt gatcctttac accatgtgta cacgtgcgat gcatgaattg acccttttta
     7921 cctccaataa tgaaagtcct ttgttaaaca atatggatat ttcatcttat gagaaaaaag
     7981 tactgtaagt aaaaagacaa ctgctatcat taatgttagc agttgtcttt ttatttacaa
     8041 taataattgc agaaaaagta attcaatcat ttaaggtcat ttattctata tataaatcca
     8101 aatctgtttc aggatatttt aatttgacaa gcgaaataat cattttcagt tcttccatgt
     8161 tttctggata ttctgggtca aataccttgc tatgacggcc aatcccgcca ttgtcgatca
     8221 atcggtaaat tcgattccat tcaatgaatt ggtcaattga ttggaaattg ctgattggat
     8281 agccgccaca atccaaaatc tctgatacac gaaatgctag gtatgcacta tcgatcactt
     8341 tatttttttt cgtgtctatc ttctcatttc tttcgataag aattttttca ggacgatgtt
     8401 tcaagctcca ctctaatagt agaaagaaag cctccatccg atactcttct tcaaataacc
     8461 gaaaggctga atcagaacga cgaaaaagca gcggaatcag ataattttgt ctataaatca
     8521 gcattggtgc gcggtccacc atacactcta aataacggtt cagataccct gtaccgacat
     8581 agtttcctaa tcgatagcct tcaacgccga atcttgcgta tttttgcgct aattttgaac
     8641 aatcagttac gttcacagct aactcttcta ctttgcggta accgcttaag atacgttcaa
     8701 aaggttctaa agtgattttt gcttccatat tcttccccgt ccatcttcca catacttcat
     8761 atcatttcac atgatactta tgctctcatc ataccacagg ttgaattaga aaagaaaata
     8821 aatcacacaa aaaagcaaga caatctgtta gtgagattgt cttgcttttc gaaaaagact
     8881 gaatactttt cgttaaaact ggttgcttag tcttctaatg ccacattatg gaacacatgc
     8941 tgtacatctt ctagatcttc cagtgcatca atcatttttt caaatgtttc ttgatcttct
     9001 ggagatagtg ttacctcgtt ttgagggatc atttggatct cagcgattga aaaatcagtg
     9061 attccttttt cttttaatgc ttcttgtata ctatggaaat ccgctacttc accgtaaacg
     9121 atgatttgtc cttcttcttc cattacatcg cgaacatcga tgtctttttc cattaggtat
     9181 tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg
     9241 taagaaaccg ctccggaaac acccatgttc ccgccatttt tcccaaatgc tgcccgtacg
     9301 tctgctgctg ttcgattcac attgttcgtc aatgtatcaa caatcaccat tgagccgctt
     9361 ggtccaaatc cttcatagcg caattcagag tatgtttcat ctcctgcacc ttttgctttt
     9421 tcgattgctc ggtcaataat atgttttggt acattataag ttttagcgcg atcaatgacg
     9481 aaacgtagtt tttggtttgc atgcggatca ggatcacctg attttgctgc cgcgtagatt
     9541 tcaatcccaa attttgcata aatccggcta ttattagcat ctttagcagt tttctttgct
     9601 acgatatttg cccatttacg tcccatattt tcacttcgct ttctgatttg atagtttcat
     9661 aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg attctgtgaa gctttttttc
     9721 tttcttcctt cattcttctc atttttagtc aattatctat actcggattg ggatttgccc
     9781 gaaattctgt tccggaatat tgcctcccaa ttgatagatt tgatcacttc taactaacag
     9841 ttccgcttct tgtgcctctt tttttccgat tcgggaataa atagaaatct cttttttttc
     9901 ttttaagaat cttcttccag catcggtaaa tccaagtagt cgaagataat ttttattttg
     9961 ttggacagga atctcttgct ttttgatatt cagcaagaca taacaagata atcgttggat
    10021 ccgtgtccat gtatatcttt ttgtttttac ctttgacaca aaatcctcaa aactatcagc
    10081 agttgcagct gctgttttca tgcgcgattc tattccttcg gtcatctgat agatatctgt
    10141 cagttccagc aaagtagaag ataacaaccg ataacgcaaa tacggccaat aagattccca
    10201 agtctgcagc gaacaagttg aaagatcttc ttctgtctga ggcggcacaa gtccagctat
    10261 tctttttcct tcttctaacg catggcgaat ggctgttgca ctggctatcc tctgatctgt
    10321 gatgattttt tcatgatagc ctgcactttt acgtcggatc ggatgtaatt ccatagggtg
    10381 gacatacttg gcattttcct ttgcataact taatcccaag atatgatttg gcgaagaaaa
    10441 atcaagcgta atatcgggat acagactgcg aaaaacctca gtcatttttt gcggataact
    10501 atacgtttga tcagacagtt cttgaaatgc ctcttctatc ctatcttgat tctcttggac
    10561 aaagcgtccg aaactgctgt aatcaaacga agtctcttct tctgttccaa aacacaaact
    10621 gtcacagtcc agtgtttgca agatttttac tgcaccttta gcaaaatagt cagctgactg
    10681 aaccgcccat tcgacgggaa gctccactac taaatccaca ccattttgta aagcttcatt
    10741 cgcgcgatgc catttatcga tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat
    10801 tacagcaatg acgacctctg ctcctgttat ttttcgtgcc atttccacat gatagcgatg
    10861 tccattgtga aaaggattgt actctacgat aatcccgcag cttttcatgc gcatcctcct
    10921 tcatagttat cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa
    10981 tacccgaact atataaacta ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact
    11041 gcaagagcaa tgcctatatc gttcggatat ataatttatg ttgaagattt attcttttct
    11101 tgttaactcg agcaggcaaa accgctagtc tttgtatgct acaaagaacc agcgctttga
    11161 tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct
    11221 gttttccagc attcttaaat aactttccaa tggataggta cgctcttgat gtagttcatc
    11281 ttcacgaata aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc
    11341 gatgctgtgt ttctcgcttc ctggataact atcccacaaa aaagcaaaat tatccccttg
    11401 ataatgatag ctatattctg gaaaaacctc atctacttga taggtggagt gtacgtcaaa
    11461 taaaaatcgg ccattttctt ctaacgcttg atagacttca tcaaacactt gctgtacctc
    11521 ttgtctattt ttcatatagc acagcgaatc agaaaaacag gtaattgcct gatattgtcc
    11581 gatttctgat aaatcgagca tattgccttc cacaaattgg atcgatgcgt cttcttcata
    11641 tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg tctaaccctg tcactttata
    11701 tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg catgccagtt ccaaaatatt
    11761 ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac cattgctgat aaagtgtttc
    11821 gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt aatccaacca
    11881 ttcactaaga tcgactagcg gagcatctga ccatagtttt tccagattat agaaatttcg
    11941 ttctggcgca tggaaaacat gtacgatcac atctccaaga tcgatcaata cccattttcc
    12001 gccttctttt ccttcaatac gttttacttc atatttgttt tcttcttctt tatcaatgat
    12061 ttcttctgta attgcgttga tttgacgttc gctatttgct gaacagatca tgaaataatc
    12121 tgccaacaag gaaacatttc gaacgtctaa agcgataatg tcttctgccc gttttgaatc
    12181 tgctgcttca atcgcaattt ttaattgttc tttactatct atgatgattt cctcctaata
    12241 tgagcagctt atttttttgc tacccagtaa ttatatgttt cgattgtttt tggataaatc
    12301 tgttgttctt gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc tacagcttcg
    12361 tctagatcag cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt
    12421 tcaatatagt ctgctacata aatgattttg tcaaggagac tcatcttagc agccccagtc
    12481 gtatgcaccc ggattgcatg aagaatctct gcatcggtga ttcctaattc ccgctctaca
    12541 aacgaagcgc caacaagacc atgccagatt gcattattat aattcaataa ctccggatca
    12601 aaaccgtctc ttacgattac cattttgaac tcctcatctg gtcgttcttt tgcataatca
    12661 tgtgtcaatg cagcaatact agctttttca ggagaagcac cgtatttttt ggcaagagcc
    12721 acggctgttt cttctactcc aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt
    12781 attttttgca tcaggttttc tctagaataa gcagtatatt cattactata gtccatattc
    12841 atacaaccct tcattttgaa tataatcaat tactttatct ggaacaaggt agcggataga
    12901 acagccttct ttgatctttt gacgtatttt cgtggaactg atgtcgatct caggtacatc
    12961 tacccaaatt acaggatatg gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa
    13021 gttgaccata gaagtcagct catcgatttt ataccatttt ggcaaatatt ctaccatatc
    13081 cccaccgata ataaagtaat aatctgtatc cggattgttt tgcgtcaatt ccttcatagt
    13141 atcataggtg taacttttcc caccacgagc taattcaatc gtttcaatct gcaaaaaagg
    13201 attgtcttcc accgctaatt ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt
    13261 ctcatcaaca tgaggtggct gataagaagg catcaaaaat acgcggtcaa gacctaaatt
    13321 ccggccagct tgttcagcca tcactaaatg tgcaaggtga acgggattga atgttccgcc
    13381 caagatccct acttgctttt tcttctctaa gaattgaggc atttcttcag gaaataattg
    13441 tgaacgaaca aatgtcttag cttgcgtatt catcatggtc actccttata ttgcttttac
    13501 ttcttttgat agcctttgat acttttcttt tgtggaaggt ttgtataata caataacacg
    13561 tccaatgatt tggacgattt cgcaatctat ttctttttct aatacttgag ctgcttcttc
    13621 tgcaatctca tctgtatttt gaagtaacga tactttgatc agttctcttt tttctaaagc
    13681 ttcttcaatt tgaacgatca ttgctgaatt gatcccacct tttccgattt gaaaaatcgg
    13741 ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta aactcataat
    13801 tttccttctt ccttaaatca atgctttgcg tttcaataca tctacaccct ttggcgccca
    13861 gcccgcaatt actactggcg ctgttacagt gatccagcct agaccggcaa aaacgatgtc
    13921 tgttttttct ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct cgacttcatc
    13981 aggacgagga ggctgtaata atccacctac atgtttttga taaaattcgt ctgctttttc
    14041 cagtttcgtt cgatgaatat ttacatcatt tgacacataa gcaataaatg aagcacgatt
    14101 gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag
    14161 ctgataaact ttaggtttga tttcttttaa cggtgcaatg atccgcaaat cttttttacc
    14221 taagtagtgc gccatttgat ggcgatggat aattcccggt gtatcaatca aaaaatgccc
    14281 gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat
    14341 tacatcttgt actcccgctg tatttttgat gatttgattg ataagcgtcg attttccgac
    14401 atttgttact ccgacgatat agacatcccg tccatcgcgg tattcttcga ttttttctaa
    14461 tagttccgtc atctcatgag atttcttagc actagttaat aatacatcta ctggacgaat
    14521 tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat ttagtttttt tcaatgattt
    14581 aggtaatata tccactttgt tcccaactaa aagtactgga ttgtcaccga caaaacggtg
    14641 tagtcctggg attagtgaac cattgaagtc aaaaatatcc accacattga cgatcaatgc
    14701 atctttttgc cctaattcat tcagtaagcg caaaaagtcg tcatccgtca atgagacatc
    14761 ttgggtctca ttataatggc gtaatctgaa acaacgctga caatagactt ccccggaagc
    14821 taaacctttt tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg
    14881 tataacggca ccacagccga tacattgtaa ttcttctgtc attcgattcc acctcgccag
    14941 cccatatcag ggtgttttgc taatagatag gccataattc ttttttccca aaaacgatta
    15001 aattgtgtct tccaactatc tgttgcgata atgggtttta caaggatgct acgtatccct
    15061 gcacgattgg ctccacgtat atcagtcata atctgatcgc ctaccattac tatctcatca
    15121 tctgctaaat ccaattgttt ctttgctatg ttgatccctc ttgctaaagg tttcattgct
    15181 cgtgcaacat acaaaagatc aaatttttca atcgctcgcg cgacacgttt tgaattattg
    15241 tttgaaacaa caacaacagg gattcctgca tttttcattt ctaaaatcca agtaagcaac
    15301 tcttctgttc catctggatt attccatgca atcaacgtat tgtctaaatc ggtcaatact
    15361 gctttgatgc ctagattctt taattgggca ggtgtaatct gatagatggc ttctaccatc
    15421 catgttggtt tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac
    15481 ataaagtact cctcaacccc gtattatact gcatatcagc aaaaaaaact acctctatac
    15541 tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa gattgatcac
    15601 agaaaaattt agaaacaaaa aacaactaat attggcggaa cctcttgtat cgtttttcca
    15661 gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca
    15721 taatgtgtgc gactttatat cgtggcaact gctcgccgtt gaacgtttca ggaataactc
    15781 gatcaacgat tcctaactcg cgtagttctg gcgcagtaat tttcatcaac tcagctgctt
    15841 ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag
    15901 cataaatcgt ttcttccagc atccagactt catccgctaa acctagagca agggcaccgc
    15961 cacttccacc ttcaccaatg atgatggcga tgatcggtac ctttaaatca gacatttcga
    16021 gcagatttct ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca ccgcaatatg
    16081 cacctgctgt attgatcaag gtaatcaccg gtctattgaa cttttccgct tgtttcatca
    16141 gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc aaaatttctg cggatatttt
    16201 cctgcagatc ccgtcctttt tgaataccga tcactgttac cggtttgcca tctaatcgag
    16261 cgattccacc aacaatcgct tcgtcatctg caaataaacg atcaccatgg cattcctcga
    16321 aagaatcgaa gatctcttca aaaaaatcca agcttgtcca tcgttcttta gctcttgcta
    16381 actgaacgac atcatgtgct gtcttatcca cgatctatcc atcctttctg tgtatgcaat
    16441 tcaagaagcg tatggatttt ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa
    16501 ccatgagata ataagaattc agccttttgg aaatcttctg gaagttcctg cttgatcgtc
    16561 tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc tagaataata
    16621 tcgccttcca ttgcaaaact ggcagttact cctccggtcg tcgggtccgt taaaacagtt
    16681 agataaaaaa gaccttcatt gctatgccgt ttgacagcag cagagatttt tgccatctgc
    16741 attagagaaa agattccttc ttgcatccgt gcgccacctg aagctgtgaa caagacgact
    16801 ggaagacttt cctttgttgc tcgttcgaat aatctggtga tcttttctcc gacaatcgtt
    16861 cccatacttc ccatgataaa attaggatcc atcactccaa taatgaaggg aatcccctga
    16921 atggttgctt taccagtgag gactgcttca tgaagtcctg ttttatcttg cattttttta
    16981 attttttccg tatattcggg aaatgctagc ggatctttgg ttacaagatc cgtatcccat
    17041 tcttcaaaac ttttttcatc aataatcaaa gctagacgct gccatgcacc gattcgaaaa
    17101 gcgtatccgc aatgagggca tactttttct tcaccgatgt ctttcgtata aaggatatgt
    17161 ttgcaatttg gacactttgc ccacatgtta tcaggaacag atggcgagtt agcaggtttg
    17221 ctctctctat taggattgat ccgaatatat ttctttttct taaacagagc catctatgcg
    17281 cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg
    17341 ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc
    17401 gtgatgattc cttctgtgat aaattcactt aaggctcgct gcatcttcat taatgcttca
    17461 aaacgcgtat ttccatggac gattattttt gcaatcattg aatcataata tggcgggatc
    17521 gtatatcccg ggtaaactgc actgtctact cgcagtccca tcccacctgc tggaagcagt
    17581 aagttttgga tcttccctgg agaaggagca aaatggaaag caggattttc tgcattaatc
    17641 cggcattcga ttgcgtgtcc ttggaatact acttcttctt gacacacatt caaaggctca
    17701 ccggctgcta tctccacctg tttttttact aaatcgattc ctgtgaccat ttctgttact
    17761 ggatgttcca cttggattcg agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc
    17821 atcagaaact cgatcgttcc ggcattttca taattgactg ctttagctgc tcgaacagca
    17881 gcgtctccta gtgcttctct ttttgtttgt gagatcacta cagaaggaga ttcttccagt
    17941 actttttgat tgttgcgttg taaagaacaa tctcgctcgc ctaaatgaat gacatgacca
    18001 aatcgatcac ctaagatctg aacttctata tggcgagcag gataaatgat tttttccaaa
    18061 tacatatcat catttccaaa tgccgctgct gcttcttgct gagctgaaga aaaatgctgt
    18121 gggagctctt cttttgattg aactttccga atgcctttcc cgccaccacc tgcagctgct
    18181 ttcagcatca ctggataacc aattctgtct gctatttcca atgcttcttc tactgtagat
    18241 aatacaccag tacttcctgg aatgactggg acatttgctt cttgcatcaa ttgacgcgca
    18301 ttgatcttat ttcccattgc atcgatcgtt gcacttttag gaccaataaa tgtgatattg
    18361 cactcttcac acatggaggc aaatcggcta ttttcagata aaaaaccaaa tcctggatga
    18421 attgcttcgg cttttgtcac gattgcagcg ctcagtactt cctgaacatt cagatatgaa
    18481 tcaacggctt ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt atgaagtgct
    18541 tcggcatctg cttctgaata tatcgctacg gtttggatcc ctagttcgcg gcatgcacga
    18601 atgatacgta cagcgatttc accgcggtta gctatcaata ctttagaaaa catatttcac
    18661 ctatccaatc ataaaggtta attccgcttc ggctactttt ttaccgtcta ctttagcgat
    18721 tcctttgcct attccggctg aagctttcac tttcaatatc tcaacttcaa ggtataaggt
    18781 atctcccggt gtaactttct tacggaattt tgctttgtcc aatccgccga aataagccgt
    18841 ctttcccttg aattgttcaa gagaaagtaa tgcaactgct cctgcttgag ccattgcttc
    18901 aacaatcaat acccctggca taacaggttc ttctggaaaa tgtccttgaa aaaagggctc
    18961 attgatcgtt acatttttct tagcaacgat ccgttctcct tcaaccattt cttccactcg
    19021 atcaatcaaa agcattggat aacgatgtgg aatgatttct tttatttctt gtatgttcat
    19081 tgttttccct ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg gctgtccata
    19141 ttcgacaact gactcgtttt ctacaaggat ttctgtgatc actccgtcgg cctcactagt
    19201 tatctcattc atcagtttca tcgcttcaat aatgcagata acttctcctt tggcaacttg
    19261 atcacctact tttttgaaag ctggtttgtc gggcgctggt tgcagataaa cgattcccac
    19321 gattggcgaa acaacctcga ttccatcaaa tactgccgat tctttttctt tcattatttc
    19381 tggagcagtt gttgcttgtt caggtatgac attgactggc tccgaaactg gcaacgtttc
    19441 gtttgtttga accatattca tgcctcgact tgttttattt ttattcatat acaattcaaa
    19501 ttgaccttca cgcaaatcga attccgttag cgaggattga tcaaattgac tgactaattc
    19561 tctgatttca ttgatatcca aaattattcc tcccagcgtt tcatgcacag aaccgcatta
    19621 tgtccgccaa accctaatga attactcatt gcataagtaa aacttgcagg tttgctttcg
    19681 tttttgacaa ctgtcaagtc aatctcagga tcttgttttt ctacattgat cgtaggtggg
    19741 ataaattgat gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc
    19801 aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa attttcacct
    19861 aatgcgattt ggattgcttt tgcttctgcc tggtcattgg ctggtgtact tgttccgtga
    19921 gcattgatat atccgacttg ttccggctgt acaccagctt cttccatcgc aagcttcatt
    19981 gctttagatg caccagaacc atctggagta ggagctgtca tatggtaagc gtcacagttt
    20041 gcaccgtatc caacgatttc tccatagatt ttggcaccgc gagcttgtgc atgttctaaa
    20101 gattcaagaa ctagaacgcc tgctccttca cccatcacaa aaccattccg attttcgtca
    20161 aacggaatcg aaccacgatt aggatcagtt gattctgaga gtgctgttaa agctgcaaat
    20221 ccagcaatcc ccatctcaca aatcgttgct tctgttcctc cagcaagaat gacatcagaa
    20281 tagccatgtt tgatgttacg gaatgcttcc ccaattgaat tcgttccgct agcacaagct
    20341 gttacgatag ctgtacaaat accttttgca cctacacgca aagcaatatt tccagctgcc
    20401 atgttggcaa tcgccattgg tacgaacatt ggagaaactc gttttgcacc tttttcatgt
    20461 agacgaataa cttggttttc gattgttgga agaccaccga tcccagatcc gatgatgacg
    20521 ccaaaccggt cttgattcat tttttcttta tctaaaccgc tcatctccat tgcttctaaa
    20581 gcagcgtaga ttccatagat cgaaaacata tccattcgtt tgctatcttt tttaataaaa
    20641 tatttatcgt aagggaaatc tttgacttca cccgctaagg taatccctgt ttctgaagca
    20701 tcaaatttag tgatcggtgc gatgccattc gtcccctctt ttaagctatt caaaaagctg
    20761 tctgcgtcat ttccaatagg tgaagtgact ccatagccag taattaccac tcgattcatt
    20821 tgtttgctct ccttttctta tccgtgcatg accaatccgc catcgacatt gatgacttgt
    20881 ccggtgatat atggattttg tgctaagaaa actgctgttt gtgcaatatc ttctacttgt
    20941 ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct tgactttgtc tgctaaaact
    21001 tccgtcatat cagttgtgat gaatcctggc gcaatggcat tacatgtaat gcctctagtt
    21061 gcagcttctc gtgccacaga ttttgttaat cctactacgc cagctttact agctgcataa
    21121 tttgcttgac caatatttcc aatcaaccca gatacactag agagattaat gatggcccct
    21181 tctcgctgct tcatcatttt tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa
    21241 ttgatatcca gacatttttt gaaatcttct gcatccattc gcatcacaag tttgtcattt
    21301 gtgattccgg cattgtttac taacacatgg atcgatccaa gcttttcttc tgcttctttg
    21361 atcatttgtc ccgctttctc ataatcagag atgtcaccgg aaactcctac gcattttaca
    21421 ccgaatgctt cgatttcttc tatcttttct tttggaatct ctccgcgacc attcaaaatg
    21481 atatttgctc cagcttttgc aaaagctaag gccatcgctt tgccgattcc tcttgtacta
    21541 cctgtgataa atacattttt tccttttaca tccataacgt tcctccttat agtattgcta
    21601 ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac tggtattgtt ttatcgattt
    21661 ttttcataaa tccgcttaat acttttccgg ggccgacttc gatgacttgt tcgatgccga
    21721 ttgtttttaa cttatggata ctctcataga aacgaacagg ttgcatgact tgctgttcaa
    21781 gtagtcccgc gattgtttct ttcttcatta tttccgttgt tgtgttgctt atgataggaa
    21841 aagaaggttc agagaattga atctgactta ggtccttcgc cagttttttt gctgccggtt
    21901 ctaaaatagc tgtatgaaaa ggcccgctga catttagagg gatcattctt ttgaaccctt
    21961 tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca atcacgatct
    22021 gctgaggtgt attataattt gcaggagata cgatgccgta cttgctagct tcatgacagc
    22081 tttcctcgat cgtttcaatc ggcgcattca tcactgcaac cattttcccg cttccagctg
    22141 gggcagcttc tgtcatataa gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg
    22201 atagcgcatt actagctact aatgccgaat attctcctaa actcagtcct aaagctgcat
    22261 ctgggatgat tccatgctcc ttcaataagc gataatacgc gatgctgact gttagaatag
    22321 ctggctgtgt atattgcgtc tgatccaaac gttcattttc tgtaaagcaa agttcagcca
    22381 tatcgtagcc gagaatctca cttgcttcat caaatgtctg ctttacaacg gcttcttcat
    22441 ataaatcttt tcccatacct tgatattgtg caccttggcc actaaataag aatgctgttt
    22501 tcatgttttt ctccaatata tccagaatat atgtttgatt cttcctattc aaaaaggccg
    22561 agacattcct atttcaggaa atgtcccagc ccccaaccta attttaatga ttatgcagtt
    22621 tgtttttcta cgtaagtcac taaatcttga accgttttga ttccgtcttc tgtttcgatt
    22681 ttcacgtcaa attcatcttc aatttcattg atgatttgga ataaatccaa gctgtcagct
    22741 tctaagtctt cttggatgtc agtagtcaat ttgatttctt cagtttcttt tcctaattct
    22801 tctgcgatga tttcttgaat tttttcgaat accataataa tatattcctc cgatattgtt
    22861 tgctttttat ttttataatt taacagtcta caactccatg agaatacttc cccatgttaa
    22921 acctccgcca tagcctgtaa atattacttt ctgctgtttt cctaaggtga tctttccact
    22981 agaaactgct tcatccaata agatagggat gcttgcagca gatgtattcc catatttatc
    23041 catattggtc aggaactttt cttgcggcat ctttactttt cgggcaattt tctcaataat
    23101 tctgaggttt gcctgatgca aaagcagata atcaactggt ttgtctttca tgacttcttg
    23161 gatattgttt gcaacgtctc gtacggcaaa atcaaaaata tctctgccag tcatttgcaa
    23221 ataatacgaa ccttctgaat cttctgaata gaacggattt ttattttgct ggtagccagc
    23281 ggtcaatgag ctacttctgg taccgtcagc tgctaatttt tctttcagca attttttctg
    23341 tggattcgct tctaagagaa tcccacctgc tccatcgcca aacaacacag cagtcgtacg
    23401 atcagaccaa tcgattagct tagatagtgt ttcaccacca atcaccagtc ccttagttga
    23461 gccgcagcgg atcatttttt cagccatgct taacgcataa acaaatcctg agcacgcagc
    23521 actgatatca aaagcgatag catgagttgc tcccaatttc ccttgtactt gtgctgctac
    23581 agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca aatctttagg
    23641 gtttagattt ttctgtttta tcaatttttc cgccactaaa atgcataaat cagaagtctc
    23701 ctgatttgtt acacatcttc gttcacgaat acccgtcctt gacgaaatcc attcatcaga
    23761 ggtatccatc atcttggaaa gatcattatt agtaataatc ttttcaggaa ggacgctggc
    23821 tgttgcagta atcattccaa attgttccat taaatatcct cacttatagt cttgcaagaa
    23881 gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt tcctccgtag ccattccgtc
    23941 taagatgttc ttgaccattt cacgatgaaa atgctgatgg acccgaaata gtaactttcc
    24001 ttttttcgtt agtcccaact ttacaacgcg gcggtcatct tcactgcgga tacgttcgac
    24061 atagcctttt tttactaact tgttaatagc agtggtcaag gtcccaacag taattgatag
    24121 ttctttggct acttctgaag ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg
    24181 catctctgtg attgacagat ctttgaattg agattttttc agctcagact cttcgattgt
    24241 caaaatatca ttgaagacac taacaagata atcatttact gtttttaaat ctggttccat
    24301 gttccaccgt ccactaatta gtttgattat caaatctttt gatgttcaaa ctatatctta
    24361 acttttttca aaatgcaacc ttaaaagatc atttttttac tatatgagct ttttttaaga
    24421 ttttactcat ttaaaacaac tgataaatca gcatttataa tttcttttat tagaaaaatc
    24481 atctatctca catattttga ttatcaaagt attttctgtg aacagaacta tagaattccc
    24541 taaaacttgt ccattatttc aaaatttgtt atagtctttt tattcaaagc gtaaaaggag
    24601 atcctatgtt aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc tatcttatga
    24661 aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa aatagagtaa
    24721 gcagctattt tcatcagaat aaacagcatt caaaaaaagt attacgtatg attcatcata
    24781 taacagattt tgatttcgtc gtagtagaca cggaattaga tgctttattg ctcgagtgtc
    24841 aattgatcca acattaccgt cctttttata accgacaaat gaattatttc agtaattata
    24901 attatgtaca tattaccaat aagggatttg tattaacaga tactcctacg gctcgaacat
    24961 acgggccttt tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt
    25021 atcaaatgcc ttggttgtct gaaattagtt tgttagcttt gcgagtccag cttcctgacc
    25081 tacaggaaat gacttttgaa cagaaaaaaa aggaactcca agggctgttt caaggccgaa
    25141 ataaaaagct actgacttac ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt
    25201 tcgaaaaagc cggtatgctt caaaaagata tcgaattagt tacttatttt attagacgga
    25261 tccaggaaca aaagcagttt ttaagaacac catcgctcac cttcagcatg ccattagcag
    25321 cagatgagag tcagaaaaag cactacctcg tttgttatgg tcaacttgca gaaacaatga
    25381 ttacctctgg tgacgtttct cctgacttct attatgaaaa gaaagaagct catttatctt
    25441 tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca aatcttgatc agttaccgca
    25501 aaaaattaga aaaagaacag tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc
    25561 aactaaatta gttgcttctc ggcttctttt aggcatcttc aacttcaaat gtcaattatc
    25621 ctaaaattta ttggattaag tcatagattt ccatcgctac gatatcgata ttatcaaatt
    25681 cgtattgctg atgagtatct gtttcagtta attcaaatgt atcggtcgct ttatcataag
    25741 ttactgtaca cttctctacg ccttcttttt caaaacggcg aacttgcagt tcattatctg
    25801 ctgactcgac catagcctcc agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg
    25861 gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa gcatagccta cttgcattga
    25921 ttttctctaa aaaaactcgt tttttatgat tttttttctg gtatttttcc ttcaggccac
    25981 caaagtttga tcaaagcttg ggttccgtca ttctcaaaac ctttatctgc caactcttca
    26041 tatagtttca atgcttgggt agttgctggc aatacaagat ccattttttc tgcttcttct
    26101 aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct
    26161 tttaaaatac gaggagaata attgcttaaa gaccagttag ctgcacttcc acctgaaagc
    26221 gtctcaatca ctttctttaa atcaagtccg tttttttgtg catagacaag catttccgtc
    26281 attcctgtca tcgtacctgc aatcatgatc tgattagcca tttttgtatg ctgacctttc
    26341 ccagcagaac catgcaatgt aaatgtcgta ccaaattctt tgaataaagg cagtacttga
    26401 tcataaacgg cttcttctcc accgaccatg atcgtcagtg taccattctt tgctcctaag
    26461 tcaccaccgg ataccggtgc atctaacgca tctgcgcctt tttcttttgc tgtttttgca
    26521 attttttctg ccaggcttgg tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata
    26581 tctgcagaaa aaatccccga ctgtccatag tagatttctt ctacatcttt ggggtaacct
    26641 accattgtaa agagaatatt actttgttct gctaccgctt ttggtgtatc tgcccagata
    26701 gctccttctg ttactaaagg atcagctttt gcttttgtcc gattatagac agctacttcg
    26761 tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa
    26821 ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac
    26881 gctagaaaca aggatttttt cataaaaaat gtttgaattt tatcttttaa atgaatttaa
    26941 ttttattttg attcgttcaa tcgagtcttc ttcttctttt cttcgcatct cttcttcatg
    27001 ataaacaacc atgtcttgat aaaattcgat catagcttcg ccgaaatgtg tagatgaaat
    27061 atcataaagt ttagcttcta aaacttttgg atctttagga acttcactgg aaatataatc
    27121 aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc
    27181 gaacaatcgg ttcaaataag catttccttc cacgacacaa ggtgttccgg ctgccatcgc
    27241 ttctgtataa gtaagccctt gtgtttctga agtagaagca ctgacaaaat aatctgccgc
    27301 tttataataa agtgctacat cattgttgtc cacttctcct gtgaactgca caaattcttc
    27361 taactgcagt tctgagatca attcctctaa ctcttctaca taaggtcctt ttcctacgat
    27421 aactaatctg gcgttaggat aagcggagat gacctgagga aacccattaa tcaaggcttg
    27481 gatatttttc tcataagaaa tcctgcttaa agaaagaatc atcaaatggt tttcctgaag
    27541 acctagtgac tcccttaatt tgctgatatc ctgctgagtg atatcagaac gtttgaattt
    27601 ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct attacaccat agctgcgtaa
    27661 tgtatcaata acacgttcac ttggacaaac tacacctgtc gtatgatttg caaataatct
    27721 agacaagtat tttacatggg attgcctgat cacttttcct ttaccgatat aatgaaggta
    27781 gtcttcatac atcgtatggt aagtatggat cagaggtatt ttcaacttct tagctaccat
    27841 cttcccaagt atccctgctc cgaattctgt atgggtatga ataagatcta gttctagttc
    27901 tttggcaacc aaataagcat accacatgcc tctaacaaca actcttcgat ccttgaaaga
    27961 aataaatggt acacttggca ttcgaatcac atctttttcc aacttatccg cattaggatc
    28021 ggtcgttgta aaaatataga cttcatgccc atgtctttct aattcttgtt ttaatgtttt
    28081 gatcgatgta gcaactccgc tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt
    28141 catgtttttc gcctccaaac tagtccttga cctgatttac tttttttaat ttgctagaga
    28201 ctttttcatt cagtacactt tcgtatacat ttttaagttc ttcaccgatt ttattgatac
    28261 ttcgctcttt tgctgtttga taaccggctt gtgtcgtact cggtagttct tgattggtaa
    28321 tctgttggat cagtcggata aattcttcgt ttgtcttccc catatagcag ttcttgctat
    28381 cttctaacca gccattatac actggaatat ctcgaacaag tacgttttgt ttgcttgcta
    28441 aagcttctaa aacaacgatc ccttctgtct cttcataaga agggaaaaag aatagatctg
    28501 cacctgaata cgcaccttct atgatctctc cacgaatgta accaggaaac aggacatttt
    28561 caggatgatc ttcttttaca attttccgga tgttccgagg aatcgagtac atgggaacat
    28621 gaccaaacca aataaatgtg tattcgggca tcttcttagc aatttctacg aaatcaatga
    28681 ttcctttacg ttcgaaaaac aaaccgacac aaatgattac tttttgatcc ggagacagtt
    28741 tgaaatattc tctgaacttt tgttctttca ttggatcagg gtgatatttc tctaagtcga
    28801 tcccattgga aatagcttga atcggcacac ggataccata gctttccaac aactgttttg
    28861 aataaggtgt tggcgtgatc agatagtccg ctttttggta caatcctacc agatattttt
    28921 ttacgatagg agaaagctgg ttcgatccga taaaggaatt tcggaaatct tcctctgtag
    28981 aatgcgcatg atagacaact ttttttcctt ctcttctggc tttattgacc atattatgac
    29041 tattgattcc gtatgtattg atgtgcagga tgtcataatc ttctttttca tctagtgtat
    29101 aagagatacc gacttccgtc agagcacgtt tttgatgatc aagcgcacgc ccgattccag
    29161 attttgcaag catcttttca ctttcaaaat ataataatac cttcacctta cgtccccctt
    29221 cacttatata gtataacata gcctacaaac tgtcactcaa acttaagccc tatttttgtc
    29281 tattcttact gacaaaaacg ttttcttaaa aaagaacaaa aaaactggcg tagctctttt
    29341 acaagccaca ccagcttttg atacgttgtt tattttacat attcatgaac aagttcaacg
    29401 acttcttcca tcgtatcgca ttctttcaat gcacgatcag caagttcagc cattttcgct
    29461 gtgtccaagc gtttcattaa actacgcgtt ttaagaatag atgtcgcact cattgagaac
    29521 tcatctagac ccatacctac tagcaatgga acagctgttt gatctccagc catttctcca
    29581 cacatacctg cccattttcc ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt
    29641 aaaattgatg ggttatatgg ttggtacaag taagaaacgc gttcgttcat acggtctgct
    29701 gccattgtgt attgaatcaa atcgttcgta cctacagaga agaaatctac ttcttttgca
    29761 aacttgtctg ctaagactgc tgctgctgga atctcgatca tgatgccgac ttggatcgta
    29821 tcagatactt ccttgccttc acttatcaat ttttgttttt cttcttcaaa gatcgctttt
    29881 gctgcgcgga attctttgag tgtagctacc atagggaaca tgatacgtaa attaccatga
    29941 acagatgcac gaagcaatgc acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat
    30001 aaactgatac gcaatgcacg atatcctaag aatggattca tttcatgagg taattgcaga
    30061 tatggtagtt ctttatctcc accgatgtcc attgtacgaa caactacagg ttttccttcc
    30121 ataccttcta atacagcttt gtaagcttca tattggtcat cttctgttgg gaagtctgga
    30181 gaatccatat acaggaattc agtacggtac agaccaactg cttctccacc attattatgg
    30241 acaccaacta gatcttttgg tgttccgatg tttgcagcta attcaaaatg cttgccgtca
    30301 gctgtgacag tttctgcatt ttttaatttt tcccattctg ctttttgtgc tgcgtaatct
    30361 gcacctgctt tttcaaattc agctttttgt tcatctgtag gatcgatgat tacatcgcct
    30421 tcgatcccgt tgacagccaa aatatcgcct tctttgactt tagcagtaat ttcttttgtc
    30481 ccaacgattg ctggaatttc taaagaacgt gccatgatag cagaatgtga agtacgtcca
    30541 ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta attgtgctgt atcacttgga
    30601 gtcaagtcat gtgcaacaac gaccacttct tcgttgatca ttgatggatt tggtaaagta
    30661 acgcctaata agtgcgctaa aatacgttta gcaacgtcac gaatatctgc agcacgctct
    30721 tgcatataag cattatcttc cattgcttcg aacataccga tatacatatc cgttacttct
    30781 tttagcgctg attcagcatt tacgctgttg tctttaatat tttgcttgat ttgacctacc
    30841 atttctggat ctgaaagaac catcaaatgg gcatcaaata cttgcgcttc tgcttcacct
    30901 aagctttgcg ctgctttttc acgaatttgc tgcaattctt cagtagattt agctaatgca
    30961 gcatccaaac gagcttcttc agccgcagta tcttctactg ttgccttact gaatgttaaa
    31021 tccggttgaa ctagcaggta agcttttgca acggctacac cgtcactagc ggcaatccct
    31081 tttagcattt caaccattat tcagataagc cttctttctt cattgtttca acgattgctg
    31141 ccattgcatc agcttcgtca gcaccttctg cagtaatagt aacatcagaa ccttgaccta
    31201 cacctaaaga cataacgccc atgattgatt tcaagtttac tgatttacct ttatactcta
    31261 agtttacatc agagttaaat ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt
    31321 ggatcccagt ttctgctact acgtgaaatt ctttcttttc catattaatc ggtctccttt
    31381 aagtgagtgt tgtttttatg tgagggtttt cttttttagt aattcagaga atcctaacaa
    31441 atgccaccct ttcatctgta aagattaaca ttttttttcg ttagatacaa cagttttcat
    31501 tcaatttcag ataaaagtat tcaaattatt ccggattctt tttcatcctt ttcttcacat
    31561 tttataacaa acgtgtttat ttcctttttc ttgtcgcttt ctcttctcct gacgtttatc
    31621 attctttcct ttgaccctct ttgttttcat atgaaacgaa ttacttgctt ccctttttat
    31681 ttttagtata ataagagtca aaggtcagga atggtcaata gagatttcac cttttctacc
    31741 tcataaatct cttaaaaaac taggggttat tcttttacct catgaaaaac gagatgtaaa
    31801 atatggatgt acccattttt gtgaaaggac gtgaatgatt catgctttgt caaaactgcg
    31861 gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa tggtcagcgg acacaactcg
    31921 attactgtca gagctgttat caaaaaatca aaaatcaaca aaatggaggt ctgactaata
    31981 tggcccaaaa tgatccattt ggatttggca gtttggacga tcttttccgt tccatgtctc
    32041 gccaaatgca acagcaagga aattatgagc aaacaccacc aacacaattt ggcggaaaca
    32101 acaacttcaa tggtggtcag ccacctcaag gcaatacaga aggtttattg ggagaatacg
    32161 gaatcaatat cacagaacaa gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg
    32221 acgaaatcaa acgagtcatt gaaattttaa atcgccgaac caagaacaat cctgtcttga
    32281 ttggagaacc aggtgttggt aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag
    32341 atggtgatgt accgcaaaaa ttaatggaca aagaagtcat tcgtttagat gtcgtatctt
    32401 tagtccaagg tactggtata cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag
    32461 agatccgtca agcagaaaat gtgatcctat tcatagatga agttcacgaa atagtcggtg
    32521 ctggttctgc tggtgatgga aatatggatg ctggtaatat tttaaaacca gctttagctc
    32581 gaggagaatt gcaaatggtt ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag
    32641 acgctgcttt agaacgtcgt atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa
    32701 cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata
    32761 ctgatgaggc catcgaagca gccgcaactt tatccaatcg ttacgtccaa gatcgcttct
    32821 tgccggataa agccatcgat ttgctagatg aaacaggttc taagaaaaac ttgacgatcc
    32881 aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga agcagaagaa caaaaagttt
    32941 tagcttctcg agaagaagat ttcgaaaaag cagcttatta tcgcgatcag atcaataaac
    33001 ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac acctgtgatt accgaaaaag
    33061 atatggaaaa aatcgttgaa caacggacag gtattccagt gggtgaatta aaagaaaaag
    33121 aacagacaca actgaaaaac ttagcagatg acttgaaagc tcatgtcatc ggacaagata
    33181 acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg tgttggatta aacaaacaga
    33241 atcgtccaat cggctccttc ttatttgttg gaccaactgg tgtaggtaaa acagagcttg
    33301 cgaaacaact tgcctacgaa ctattcggtt cacaagattc aatgattcgt ttcgatatgt
    33361 ctgaatatat ggaaaaacat agtgtctcta aattgattgg ttctcctcca ggttatgtcg
    33421 gatacgaaga agctggtcaa ttaacagaaa aagtacggag aaatccttac agtcttgtct
    33481 tgttagatga agtagaaaaa gctcatccag atgttttgca tatgttcttg caaatcttag
    33541 atgatggtcg attgacagat gcacaaggaa gaacggtcag ctttaaagat acgatcatca
    33601 ttatgacaag taatgccgga actggtaaag tagaagcaaa cgttggtttt ggtgcggcac
    33661 gcgaaggtgt tactcgttct gtattgaacc aattaaacaa ctactttaca ccagaatttc
    33721 tgaaccgttt tgacgggatc atcgaattca gtgcattatc aaaagaaaac ttgatgacaa
    33781 tcgtcacact aatgctagac gatgtcaacc aaatgctggc agcacagcaa cttcacatcg
    33841 aagttcctac gaatgtcaaa gaaaaattag ttgacttagg ctatgatcca tctatgggtg
    33901 ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga tggtatcgct gaattttatc
    33961 ttgatcaccc ttctatccat gagttgaaag caaaactgga taaagatgga aaaatcatcg
    34021 ttacttctaa accagaacgt ttggctaaag aatcagccga agaaacggct gaataaatcc
    34081 agttttatag cgtgagacgg tatttctttg agaaatgccg tctcattctt ttttcagaaa
    34141 agtcaaaata ctgttcattt tataaaaaag aaattataat aagagaaaca agcaattaaa
    34201 aatacataaa agaggtgagc gccatgaaat tggtaaatgt aacaaacagc cattcccgtc
    34261 ttgtattaaa ccagcttgaa aacactgatg cccatctagt caaggtctac acagccggaa
    34321 ataccactat tgtctacact gaagcccctc aacataatga aattctgctg atcaatgaca
    34381 aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa ttatttctta gataaaatca
    34441 aagacgccgt ttaccacgag gatgaaatca aagtaatcga attggcaggc cttgttgaaa
    34501 tctcgattcc aaaacatgaa ttaggcgcta cattagccgt gtaaacaaat tttgaacata
    34561 ataattaaag agcgtgggac aaaagtaaa
//
LOCUS       DAZKOW010000016        32816 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000016 DAZKOW010000000
VERSION     DAZKOW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.016"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..1213
                     /locus_tag="ACG912_000821"
     CDS             44..1213
                     /locus_tag="ACG912_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFQ0512162.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTP
                     EPDTDARDQGIPEKINKKTIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAET
                     LKSSKDTTFASPETKNKQLPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(1339..2418)
                     /locus_tag="ACG912_000822"
     CDS             complement(1339..2418)
                     /locus_tag="ACG912_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0512163.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2527..2967)
                     /locus_tag="ACG912_000823"
     CDS             complement(2527..2967)
                     /locus_tag="ACG912_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0512164.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3024..3434)
                     /locus_tag="ACG912_000824"
     CDS             complement(3024..3434)
                     /locus_tag="ACG912_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0512165.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3615..4049)
                     /locus_tag="ACG912_000825"
     CDS             complement(3615..4049)
                     /locus_tag="ACG912_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0512166.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(4149..4997)
                     /locus_tag="ACG912_000826"
     CDS             complement(4149..4997)
                     /locus_tag="ACG912_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0512167.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5386..7152
                     /locus_tag="ACG912_000827"
     CDS             5386..7152
                     /locus_tag="ACG912_000827"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFQ0512168.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7254..8513
                     /locus_tag="ACG912_000828"
     CDS             7254..8513
                     /locus_tag="ACG912_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0512169.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8660..9961
                     /locus_tag="ACG912_000829"
     CDS             8660..9961
                     /locus_tag="ACG912_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0512170.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            9964..10815
                     /locus_tag="ACG912_000830"
     CDS             9964..10815
                     /locus_tag="ACG912_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0512171.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10828..11643
                     /locus_tag="ACG912_000831"
     CDS             10828..11643
                     /locus_tag="ACG912_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFQ0512172.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11794..12960
                     /locus_tag="ACG912_000832"
     CDS             11794..12960
                     /locus_tag="ACG912_000832"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0512173.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13190..13837
                     /locus_tag="ACG912_000833"
     CDS             13190..13837
                     /locus_tag="ACG912_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFQ0512174.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13921..14865
                     /locus_tag="ACG912_000834"
     CDS             13921..14865
                     /locus_tag="ACG912_000834"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFQ0512175.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15000..16451
                     /locus_tag="ACG912_000835"
     CDS             15000..16451
                     /locus_tag="ACG912_000835"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFQ0512176.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16472..17308
                     /locus_tag="ACG912_000836"
     CDS             16472..17308
                     /locus_tag="ACG912_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0512177.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17308..17967
                     /locus_tag="ACG912_000837"
     CDS             17308..17967
                     /locus_tag="ACG912_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0512178.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            18093..18782
                     /locus_tag="ACG912_000838"
     CDS             18093..18782
                     /locus_tag="ACG912_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512179.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18788..19744
                     /locus_tag="ACG912_000839"
     CDS             18788..19744
                     /locus_tag="ACG912_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFQ0512180.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19788..20300
                     /locus_tag="ACG912_000840"
     CDS             19788..20300
                     /locus_tag="ACG912_000840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0512181.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20556..21104)
                     /locus_tag="ACG912_000841"
     CDS             complement(20556..21104)
                     /locus_tag="ACG912_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0512182.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21193..22374)
                     /locus_tag="ACG912_000842"
     CDS             complement(21193..22374)
                     /locus_tag="ACG912_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0512183.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22610..24118)
                     /locus_tag="ACG912_000843"
     CDS             complement(22610..24118)
                     /locus_tag="ACG912_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFQ0512184.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24277..24468
                     /locus_tag="ACG912_000844"
     CDS             24277..24468
                     /locus_tag="ACG912_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512185.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24671..25588
                     /locus_tag="ACG912_000845"
     CDS             24671..25588
                     /locus_tag="ACG912_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0512186.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25581..26654
                     /locus_tag="ACG912_000846"
     CDS             25581..26654
                     /locus_tag="ACG912_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512187.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26802..27320
                     /locus_tag="ACG912_000847"
     CDS             26802..27320
                     /locus_tag="ACG912_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512188.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27301..27693
                     /locus_tag="ACG912_000848"
     CDS             27301..27693
                     /locus_tag="ACG912_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFQ0512189.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27769..28704
                     /locus_tag="ACG912_000849"
     CDS             27769..28704
                     /locus_tag="ACG912_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0512190.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28837..29616
                     /locus_tag="ACG912_000850"
     CDS             28837..29616
                     /locus_tag="ACG912_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0512191.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29609..29926
                     /locus_tag="ACG912_000851"
     CDS             29609..29926
                     /locus_tag="ACG912_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0512192.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30170..31780
                     /locus_tag="ACG912_000852"
     CDS             30170..31780
                     /locus_tag="ACG912_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0512193.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31937..32785
                     /locus_tag="ACG912_000853"
     CDS             31937..32785
                     /locus_tag="ACG912_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0512194.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        10839 a         5875 c         6410 g         9692 t
ORIGIN      
        1 atcttatttc ttgcatacaa aaaaatcaag gagtgagaga gttatgagat attcaaccat
       61 cttaatatca acatttgcag ccttaagctt atcgcttact ataaatcaga cagtccacac
      121 agaggagata gaaacagcca aatggacaga aaccaagtgg gccaacgtca atcgaattac
      181 ctttcaggat gaattttcat ccttcaattc aggagtcgca aaattaagaa aaggacaaac
      241 agcgaccatc aaagcaaaaa ttgactattc aggagactta gcacctatac tacaatcagc
      301 tgttacattt gagaatgttg atcccgcttt gtcgattggc tatgacgaga acactttgac
      361 tatccaacac aagcaagcga tatttacctt caccgttaca ttaaatcagg acttgttaaa
      421 accagcgctt tttactgtca aggtatcaga tggactaccg aacgatcatc atcatttaac
      481 gccgtatagt cagcggcaga caattgagca agattcagct atcgactcag gaggagatct
      541 tgttgaagaa ccaacagata aaccagaaaa tgaaaataag ccagaagtac cgcctacaga
      601 aaatccagac ggagaacaga aaccggaaat agaacctgga gaagaacccg atacagaaac
      661 tcaaccagag ccagacaatg aatcaaaacc tgaaataacc cctggagaaa aaccagatgt
      721 agacccagaa gaaaaaccgg atgtgactcc agaaccagat acggattctg gaaaccaaac
      781 agtaccggaa acaaatccag acacagataa tgaaactgaa aatccagaaa aactagaggt
      841 agatccagaa gaaaaaccag atgtgactcc agaaccagat acggacgcaa gagaccaagg
      901 aataccagaa aaaataaaca aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa
      961 atcaaatcta gcaattctaa aaataaatga agagcagctg aataaaaaat cacgaatatt
     1021 tgatagcgcc caatcagctg aaaccttaaa aagtagcaag gatacaacct ttgctagtcc
     1081 tgaaacgaaa aataaacagc taccaaaatc cggagagtca caaaacaaag ttatactgtg
     1141 gagcggaatc attctattaa gtatagcaac aatgctttct gcaaaaagat ttaaacaaaa
     1201 cagatctctc taacatttga ataatgtctg tgaacgcatt tttcaaataa aaagaaatat
     1261 ccagaaaacg accccaaaat accagattga aatctgatat ttggggtcgt tcatacttta
     1321 tctttcttca tatttatttt aagggatatc tgtttctttt ggaagatggt tcgggatctt
     1381 tttcactttc aatacatcta aaaagaaggt aaagatcgga atgccgacaa tcaagcccca
     1441 aacgccaaag aaccgttcgc taacaagtaa gaccacaaat gtataaaaaa tcggcagttc
     1501 tgttttgctg gacatcaact tcggattcag cacataagac tcaattagat ggacaatcaa
     1561 aatcgtaatc aggatataaa caacatctcg gatgcctcct tgggaataag ctatgaaaga
     1621 tagaggaata caagaaatga taacacctgc tactgggatc agacttaaaa tgaaaatcat
     1681 gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc aaggcgattg tagtgagcaa
     1741 cgtgtttaaa agagcgataa caaattgagc ttctaatacc aaaccaaatg tattgacaaa
     1801 cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa aattcacttt ttaaaaacaa
     1861 tcttgagaat acaattgttt ttttcttttc aatcataaaa aagaaactta agatgaatga
     1921 catagcaaac gccactgcca tttttcctat atcctgtaaa tagccaagcg caatcgaagc
     1981 gccattttga agctgttcaa aaaaatcgtt tcgctcaata taagtatgga tatattgtaa
     2041 aacaggatct gcatcttttg gctgatgctc gtaaaaatca ataacggact ggatcatatc
     2101 aaatgtttga tgaataagca tcgggatata ctttgtgaat gccagataga taaggtaaac
     2161 gatcagtgta taaatcatga tagctaaaac acctgtaggg attcggacaa atcgctgaat
     2221 gatttgaaca agatgaatca tcaaataagt aaagataaat gtgagcaaaa tggttgtaat
     2281 catacttcta actaagaaaa gaacaaaaac gacaaataat aaaacagtga gacggcgtat
     2341 tttttcattc gccaaaaaac gctgataaac ggtcaaatcg acaacctcca aataacaaaa
     2401 aataaacacg ttattcatta tatgatggaa tatgaaaata agaaagagaa agagcaaaaa
     2461 gaaaaaccgc taaatgacgt tatcttatga gttggttttc ctcataaaat cgttcatcta
     2521 gcagtttcag ccctttttct ggtcttgatg aaaattatct agcatttttc gggtcagatg
     2581 ttgcagtgta tccaattctt cctgttcaag tggaagataa gacttgatct tttgtggaat
     2641 accttctgct tgtttttgca acgcagcacc ttgaggagtc agcgtaacat ttacgattct
     2701 ttcatcaccc gtttcacgct gacgattaat ataatttttt tctgctaact tttttagtaa
     2761 gggagtcagc gtaccagaat ccagacacaa atcatttcct agctctttca ctgaagtctt
     2821 tttcttttcc cataaaacta acatcacaag atactgggta taggtaagat tcagcggctt
     2881 gaggagggaa gtgtagtgtc taatgatttc cttagaggcg gcatataaag gaaaacatac
     2941 ttgattaact aaatatagat cttccatttt tccacctccg ctgttttact atagtgtatc
     3001 atagcggatt tacacaaaaa agtctattca ctaactgctt tcagagtaac ttctatattt
     3061 ccagctgtag cttttgaata cggacacact ttatgcgctg cctctaataa ttcttgcgtt
     3121 tcttccagag aaagaccttc gatagatcct tcaatctcta cacctaagac aaagctcgtt
     3181 tcactttctg aataaaggga aacttgtgca ctgacagtag aagccccaga gatatttttt
     3241 tgctgtttga tcaattctaa tgcactgtta aaacatgcac tgaaaccagc tgcaaacaat
     3301 tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag ctgcaatatt caattcgaat
     3361 gtacgatcgg gattatgaac ttctccagag cgtccgcctg tgttgatcat tttcgtagaa
     3421 tacatttttt tcataaaatt accaccttta attatatttt atacaattga attttacaca
     3481 atataattgt ggaaagcaaa gaaatggatt ggcataagat aaataagaga aagcacatga
     3541 aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa atatcagaga caaaaactaa
     3601 tctcatcata aacattacat atatttctca aaaagttgtt ttctttcttc aaaacttact
     3661 tcacgatgga cctgatgaag catccataaa taaccaaaag gatctctgaa cattgagtta
     3721 gtcacaccaa agtccatcat ttctgtcatt tcttgaatct ctacacagcc atttcgtaat
     3781 gctttattat gtgtgtcctc gatatcagag acgatgatat tgaaccagat cgtatttgga
     3841 tgttcagcag taggagcagt caaaccaaga gacgtgtttt catccagcat atggaatctc
     3901 gtgccataca aggtaaaaat gacttcatta ttgcctctag ggtaatctgt tgcctcaatc
     3961 agttgtattt catcaaagat tgtttgatac agttctagtg ctttcaaact atcatttaat
     4021 acaaaatcta tttccgttcc tttatgcata aagatctcct ctccttttca gtcgaacagt
     4081 tactagattc ttttattata ccagttaaag aaaacgcatt caaaaaaata gcggatttac
     4141 ttaccaactc attcgattgg atgataagta aatccgcgtc ccattacttc tgctacgctg
     4201 ctgacaatga caaaggcact tggatcgatt tcttctatca atggtttcat tcgggaaaat
     4261 tcatgatcag tcacaacgat caataaaacc tcttttgact ttcgattata cccgcctttt
     4321 gcctctagca gggtaaggcc acgtccaatc tcagcactta agcgaaggcg gatgtcttcc
     4381 aaataatgtt cactcatgac cataacagat ttctttcgat tgatacctgt ttcgatataa
     4441 gtcatgacaa ttgaagtgat tgcaatagat aaaatagcat aaagaaactc ttctattcca
     4501 aaaacaaaca gattaagaga aacaacgaat gcgtctgtaa aaaataagcc aatcgaagta
     4561 ttcaagtgga aatatttttt aaaaatgagg ggagggatcg tcgtaccgcc gctagaagag
     4621 ttgtttttat ataaaatcgc aactcctaaa gcaaaaatcg cacttccaaa aatgacagac
     4681 aacaaccgat ctgaagtaat cattgtttct ggaactattg caatgattaa aggaaaaaac
     4741 aagcttccga aaagtacttt aaaaaagggt tttttaccta aaaatagaag agctagtacc
     4801 aacataacga tattcaacac taaaataact atagctcggt caaatcctaa aacagcttcc
     4861 agtaatattc ctagaccgct gacacctcca gcagcaatat gatgaggccc taaaaacata
     4921 ttgatagcga ctcctaatat gaagagggca aaaataatct taatgtatgt ccatataaat
     4981 tgtctcgttt ctttcattta tccacttcct taattcatta ataatactct tatcttatta
     5041 taacttactt ttcaccttaa gttaacttta attaataaaa aaattaggct agtctaaatt
     5101 attatgtttc attcttctag aatatacttc tacgttaaaa actgttaaaa aacttttact
     5161 ttagaaaatt ggagaaattg atcgcttaag ataaattcag gacactaaat agtacaggaa
     5221 gtagattaaa acttttaaaa gttttaagtc tatctatttg gcaagtttaa aaaataaaaa
     5281 ttcggtatca aaaaactgtt accgataaca tttttgaaag cgttgtcttt tgtttctcta
     5341 tcacttatag tgatgacgtc atattaaaac acaaggagga aatcaatgga tacagctgca
     5401 atttatcatc gaccggaaag tgaatatgca tttctatata cgaatacaag atttcgtatt
     5461 cggcttcgta ctaggaaaaa tgatatcaaa aaagtatatg tgctttgtgg agatccatat
     5521 accataacaa ctgaaaaatg gtatcaaaaa caacgaccaa tgaaaaaatg tctaagtacc
     5581 aatgttcatg attattggga aattgaagtg acgagcgaaa caaggcgctt acagtatgcg
     5641 ttccatgttg ttggggaaga taacacggac tgtttttacg gtgatcaagg aatttttcct
     5701 tatcgagaaa atgtgattac tgaaccaaat ttttattttc gaatacctta ttttcatcaa
     5761 atcgatcgat tcactattcc agaatgggta aaagaaacag tatggtatca aatttttccc
     5821 gaaagatttg caaacgggga caaagcaaat gaccccgaac acactttacc ttggggaagt
     5881 aaagatccag accgagaaga cttcttcggg ggagatttgc aaggtgtgag ggatcatttg
     5941 gattacttga cggaccttgg cgtcaatggc atctattttt gtcccatatt taaagccaca
     6001 tccaatcata aatacgatac gattgattat tatgaggtgg atcccgcttt tggcgataaa
     6061 aaacttttaa aaaacttgat tgatgaagca cataaaagag ggatacgtat catgttcgat
     6121 gctgtattca atcatatggg tgtccattca ccacagtggc aagacgttct caaaaacgga
     6181 gaaaaatcga tctataaaga ctggttccac atccgttttt tcccagtaga ttcttatcag
     6241 atgactgact taccagaaac tgctgaaaat atcccatatg atacttttgc ttttactcca
     6301 ttcatgccga aactaaatac agcaaatcca gaagtgcaaa aatatttgct ggatatcgca
     6361 acttattgga taaaagaatt cgatattgat ggatggcgtc ttgatgttgc taatgaagtc
     6421 gatcatcatt tttggaaaaa attcagagaa gcagttacag aaatcaaacc agacatctat
     6481 atccttggag agatttggca ttcctctcaa gcttggctgc aaggggatga attccatgca
     6541 gtgatgaatt atgcattcac agattcgatt aaagattatt ttgcaaaaaa gaaaatcaca
     6601 gcttcacaaa tggtcagtgg aatgaatcat cagcaaatgc tttacagaga tcaggtgaac
     6661 gaaggaacat tcaacttatt agattctcat gatactgcca ggatcttgac cctttgccaa
     6721 ggtaacaaag aattgatgaa gtctgtgatg gcctttatgt ttcttcaaaa agggtctcct
     6781 tgtatctatt acgggacaga aatagggatg actggtgaag atgacccgga ttgccgcaaa
     6841 tgtatgatat gggagaagga ggaacaagat ctagagctat tcggttttat aaaagaactt
     6901 gtttctctaa ggaaacagtt gtctaagatt atttctgaag gttctactca gtggctaata
     6961 gtatatgatc gtgaagataa gctctatttc acacgggaac tagagggaca gatcatctat
     7021 gtttacttta atcagtcaaa agaaccttgg gtagtagaac aggaaaatga ggtgattctt
     7081 agtcaaaatt gtcagttgtt agaagacggg aaagcagaga tacgacaata cggatttttg
     7141 atcatgtgtt gaaaactgtt accggtaaca acaagaaagc gtttgcaaat gaagatttgt
     7201 gcccattata ataagggtgt aacagcacgt tgaatacaca ggaggaaatg aagatgaaaa
     7261 aaagaagtgt gaaacagttg ggtttagggt taatctcttt aggaatctta ggcggattag
     7321 cggcttgtgg caatggtaac tcttcagcag aaggggaagc cgatgaagga aaaacgttaa
     7381 cagtttccgt tgattctgga tataaagatt atataaacga aataaaaaac actttcgaaa
     7441 aagaaaacga tgtgaaaatc aagctagtag aaaaggacat gttcgatcag ttggaatcat
     7501 tagcgttaga tgggcctgct ggaaagggac ctgacgttat gatggcagca tatgaccgga
     7561 tcggtgcgtt aggacagcaa ggacatctgg cggaagtaaa actgggaaat gaagcagctt
     7621 acgatgaaac agacaaagcc caagtgactt atgatggaaa aatctacgga gaacctgcag
     7681 taatcgagac ccttgttttg tactataaca aagatttagt cgatacagca ccatcaacgt
     7741 ttaaagattt ggagaatcta tccaaagaca gtcgttttgc ttttgagtca gaagcaggaa
     7801 aaaatacagg atttttagct aaatggactg acttttatta ttcctatggt ttaattgcag
     7861 gttacggtgg ctatgtattt ggtgatgatg gcacaaatcc ttcagatatc ggtttgaata
     7921 atgcaggtgc tgtagaagga atctcttatg caacagattg gttcaaaaat gtttggccaa
     7981 agggaatgca agacataaaa agtgcaggag acttcgccag ccagcaattc atgtccaata
     8041 aaactgcagc aatcattgac ggtccatggc aagcgcaaac atacaaagaa aacaatatca
     8101 attatggtgt agccaaaatt cctactctaa ataatggaca gccttatcaa ccgtttggcg
     8161 gaggaaaagg atgggtagtc agcaattatg ctaaaaacaa agacctctct caaaaatggc
     8221 ttgattatgt aacgaatcag gaaaatcaag aaaaattctt tgaaatggta aatgaaatcc
     8281 ctgcaaacca acaagctagg gaaacagcaa aggctaaaaa cgatgaatta acaacagctg
     8341 tgattgaaca atatgaaaca gctcaagcga tgccaaacat cccagaaatg ggcgaagttt
     8401 ggacaggtgc agaaaacttg atgttcgatg cagcatcagg ttcaaaaaca ccaaaagaat
     8461 ccgcagatga agcagtaaaa acaatcagtg aagccataga acaaaaatac tgattaaaat
     8521 gataaccgtc catttttata aaaaatggaa caggtgcttc agataaaagt ggcaagagcg
     8581 agatggaaaa ttatcttgct cttgttgcca ttatcttaaa agcagtttgc ggtttagcaa
     8641 gtttagaggg ggcattcaga tgcagcaaga taaaacaagc aatatcaacg aaaaaaaaat
     8701 cacgctttgt tcacttattc cagggatggg gcagtttgta aatggccaag cttttaaagg
     8761 tatcgttttt ttcgccatat tcgtcctatt cattggtcag atgcttctgg gcggatttca
     8821 tgcattggta ggattggtta ctttgggaag tataccaatg gaagatcatt cattatttat
     8881 tttgattgaa ggcactttgc aattcatcat tacaatcatt ttcttgattt tttacggttt
     8941 gaatctttta gacgcttatc gagtagcgaa gatgcgtcaa gagggcaaaa aaatcaatcg
     9001 gacgatgaag gaagtcatac ataatatagg tgatgaaggt tttccttatc tattgacact
     9061 tcccgcttat cttctcatga tttttacgat cattttccca gtcctagtta cattgttcac
     9121 tgcatttact aattatgact tcaatcatat tccaccagcc agtttaattg attgggtagg
     9181 ggctaaaaac ttttttagta tcttcttttt aagctcatat cgtaatactt tttttgcagt
     9241 cttttcgtgg accgtgattt ggactttatg tgcaacaact ttgcaaatca cccttggtat
     9301 tctcacagcg gttgttgctc accagaaatt tattaaattc aaacgtattt tcggtatcat
     9361 tttcttatta ccatgggcgg tcccagcttt tattactatc atgagttttt cgaatatatt
     9421 caacgatagc gtaggtgcca tcaatactca agtgattcct ttcataaacc agcttccttt
     9481 tgtagatatc agtgcaatct cttggaaaac ggacccgaac tggacgaaag tagcaattat
     9541 cttagtgcag ggctggttag gatttcccta tatctatgtg atggttacag gaatcttaca
     9601 atcgatttca gaagacttat atgaagcagc aaaaatcgac ggagcaaatg catggcagcg
     9661 cttttcaaac atcacgcttc ctgctgtctt cctagtggca gcgccaacat ttattacaca
     9721 gtatacgggg aatttcaata atttctccat gatctatctg tttaacaatg gtggtcccgg
     9781 aagcgtaggc ggaggagctg gttctactga tattcttatt tcctggatct ataaacttac
     9841 gactggaact tctccgcagt attctatggc ttcagcaatc acgttgatta tctccgtcat
     9901 cgttatctgt gtgtcattgc tggtatttaa aaagacaaat gcgttcaata tgggggatta
     9961 acaatgaaaa aatttcattc gagtgctcga ttccaaaaac gactttctca cttttttact
    10021 tacttttttc ttttaatact gtcagtcatt atcatctatc cgcttttgat cacagcaagt
    10081 actgccttca agtcaggaaa tattgcagct tttagtttag atctttcaag tgactggact
    10141 ttaggaaact ttactcggct attcagtgaa acactttacg ggacttggta taaaaataca
    10201 ttgatcattt ctgtcctaac aatgatcgta caagtgactg tagtaacttt ggcgggctat
    10261 acttatagtc gctatcgttt tatcggccgc aaatcaagtt tgatgttctt tttagtgatt
    10321 cagatggtac cgactatggc agcattgact gccttttatg tcatggcttt cttattagaa
    10381 gcgctcgata agtactggtt cttgacaatg atctatatag gcggtggaat tccaatgaat
    10441 acttggctga tgaaaggata ctttgacact gtaccaatag atttagatga atctgctaaa
    10501 cttgatgggg cagggcattt caggattttt ttccagattg tattaccgct tgtcaaaccg
    10561 atgctcgccg ttcaagcatt atgggctttt atgggacctt ttggggattt catgttagct
    10621 aagttcctgc ttcgaacacc tgaaaatcag actgttgctg tcggcttaca aacatttatt
    10681 tctgatgtga caaaccaaag agtctctctt tttgctgctg gggcaatttt gattgcacta
    10741 ccgatttgtc tattattctt ctttttacaa aagaactttg ttaccggcct gacatctggc
    10801 ggaacaaaag gataggagca gagtatcatg caaacatttc cgttaaacta tttttccgca
    10861 ttacgtacgc caactcaagt gtttgcggga cgcaaactat tatcatggcc taaatttttc
    10921 ttgatatttg tatttttagt ttccttgatg gtcatgcctg tgactctttt ttatgccaat
    10981 cagatccaag cgattccatt agaacaattt ctatccgttc attcgctgat cgatgaacaa
    11041 ggaacccaaa aattttctga acttgaatta tctgaaactg ggttacaagc atcgcaacaa
    11101 acgatagctg tgacccctga aattttggta ggtgtgtctt tatctgagaa acaacaatcc
    11161 gatcatggaa cttttattga ttttgagaag gaacaatggg tgatccaaca aaaggataaa
    11221 tctggtatac gccgctatac gatgaattat agcccatctt tccaaccaga ttctgtacgt
    11281 acgcccgaag atttccaacg atttttagaa agagaatttt atgccagtaa tcgtccaaca
    11341 atcattttat cttatagttt aagtcttggt cttgtcctat ttgtcatgac gagtctcatt
    11401 ttattcgggg cttccttttt cttgtggatg acaagaaaaa gccaactttc atccattcat
    11461 acattcaaag agagtgcaaa tttgatgctg aatgttatgg gaataggttc catggcagca
    11521 gcagttgtag ggtttatcca ctttgacttt atcttgatgc taagtgtcca aacaacgata
    11581 actgtcttgc tgcttttatg ggtttttgtc aaaacaagat tcaaagataa gcgtgtcgaa
    11641 tgacaagttt aagcgattta tgcgacaatg aatgtgaaaa aaatgaaaaa taaagtagtt
    11701 tttgaaaaat ttctgaaaaa attaaacttc ttagttgatt cttttctttt ttagtattaa
    11761 cgtaataggg tagttttttt aaggaggaaa atcatgttag agaaaacttt atacaatcaa
    11821 ttattttcac gttctttctc gctccctgtc gaagtaaatt attgggacgg tacaacgaaa
    11881 caatacggag atacggacac tcctccccaa atcaaaatca atatccatga agaaatccca
    11941 atgaaagaga ttacaagtaa cgcttctttg gcactaggag aagcctatat ggatcatcga
    12001 atcgagatcg agggaagtat ccaagcattg atcaacgatg cttatcagca agcaaacagc
    12061 tttttacgag gaaaagatta tctaaaatgg ctgccaaaga aagaaaaaca tacgaaacaa
    12121 aaaaacaaag aagatatcca tgcgcattat gacttaggta acgactttta taaactttgg
    12181 ctagatccaa ctttgactta ttcttgtgct tatttcaata cacctgaaga tacgttggaa
    12241 caagcacaaa tcaataaagt ccatcatatc ttagacaaac tctttatcaa agaaggcgaa
    12301 acgttgcttg atatcggttg tggctgggga acattgatgt tcactgctac caaagagtat
    12361 aatgtcaagg caactgggat cacactaagt gaagaacaat acgatttgat taccaaaaga
    12421 ataaaagaag aacatttaga agataaatgc cgtgtattat taatggatta ccgcgaacta
    12481 aaaggcgaaa cgtttgatca tatcacaagt gtaggtatgt tcgaacatgt aggttctgaa
    12541 aatcttgaag gttatttcaa agtggtaaaa gatttattga aaccaaaagg aacagctttg
    12601 atccatggaa tcagccgaca acaaggcggt gcgacaaatg cttggatcaa taaatatatt
    12661 ttccctggcg gttatgtccc tggtgttact gaattagttg ataatattac aaaaaacgat
    12721 ttgcaactga ttgatttgga aagcttacgt cgtgactacc aattgacatt agaacattgg
    12781 acaaagaatt ttcatcaagt ccaagatcaa gtcacgaaag aaatgggtga ttccttctat
    12841 cgaatgtggg atctatattt acaagcttgt gcagcttcat ttgaatcaag taatatcgat
    12901 gtgattcaat atttgcttgt tcaacctgga aacaatgaca tcccaatgca tcgtgcataa
    12961 ttgaggcaaa agtactgttt aaagactatt taaaaagaag ctaagtttta tgatgctcca
    13021 gaagtgaact tttttgtcta acttctagga ttgtctctaa agcttagttt ctttttcttt
    13081 atattttcat aataaaagtt tggttcttta atttttaaac atgacagaaa attttttctt
    13141 cgttatactt gtaatagaat actattttat aacaaagggg atcgaatcaa tgaaaaaaac
    13201 tgttatttat actagtttat ttgcagcttt gtttttagct gcttgtcaat caaataccaa
    13261 tcaaggcaca tctgggaata cgtcttccag tagcgaacag cgctcttcac aggtatcgga
    13321 ctcctcaaca aaagcaagca catcttcgtc tagccagaca aaaagttcta gtgaagcaag
    13381 tactcagtca gatatcaaag tgtctgcagc tcgtgcgatc gaattgttcc aagataaata
    13441 cccagatact gcaattacta gtttggaatt agatagtgat tggggatctt acttctacaa
    13501 gatagaaggt gtcgatgatc aaaaagaata cgaagcaaga attgatgctc aaaatgaaaa
    13561 aatggagaca gagaatccag aaactctaga tcgagatgaa caaaatggtc aaaagaaaaa
    13621 agaagatggt ctggatgttg ataaattgat cacgattgag gaagcagcaa aaatcgccgt
    13681 cgacaaagta ggaagcggta cggcaacaga ttgggatttg gataaagatt taggtactac
    13741 ttattgggaa gtcaaagtaa aagacggcaa tcaaagtaca tcagtcaaga tcaatagcca
    13801 aacaggcgaa gtactgtcaa ctgaacaaga tgattaagaa tttcttcgga aaacaagagt
    13861 tgatttttct tttagtcatg atacactaga caaaataact tgtttgaagg agcgatttac
    13921 ttgagtttag aaacgacctt tgccaatcat atctttgcca atcctttgat gaatgcatct
    13981 ggtgtccact gcatgactac ccaggaatta gatgaactgg ctcattcaga agcaggagca
    14041 tttatcacta agagctgtac gatcaatgaa cgaaaaggaa atcccgaacc acgctatttt
    14101 gatgttcctt tagggagtat caattctatg ggacttccaa acttaggttt ttcttattat
    14161 ctagagtatg ctcttgcgta tgaaaaagtg caggaaaacc aaaatcaacc gttatttttt
    14221 tccattgccg ggatgagtgt ccaggaaaac ttggaaatgc ttgagaaaat agaaaaaagt
    14281 gggtttaatg ggatcactga attaaattta tcttgtccca atgtgcctgg aaaaccacaa
    14341 ctagcttacg attttgaggc cacttatgaa acactgaaag aagtattcag catcttttct
    14401 aagccgctag gaatcaaatt gcctccttat tttgattttg ctcattttga tcagatggct
    14461 gatatcttga atcagtttcc attgacctat gtcaatgcta tcaacagtgt gggcaatggt
    14521 ttgtatatcg atacagaaca agaagcagta gtcatcaaac caaaagaagg gttcggcggc
    14581 attggcggag agtatatcaa gccgactgct ttggcgaatg ttcgcgcttt ttatacacgt
    14641 ttgaaaccag aaatccaaat cattggtaca ggtgggattc ggacaggaca agatgcattt
    14701 gaacatctgc tatgtggcgc aagcatgtta cagattggta cagagcttca taaagaaggg
    14761 ccggaaattt tttctagaat cataaaagaa ttaacgcaga ttatgtcaga aaaaggatat
    14821 acgtcaattg acgaatttaa aggcaaatta cgcaccattt catagcaaag agacgaaggt
    14881 catgattgat cttcgtcttt ttttataaaa aacactcgtc tgttcattcc tgtgcaagcg
    14941 tactcattgt atgttagacg aacttgtggg aaaataacag aggcaaaaag gaggataata
    15001 tgaatgatgc aacagaatta gcaaaagagc ttaaagaaaa agaatttagt tttcaagaac
    15061 tagttacgga tatacagaaa aaaataaaga agcaaaatca tgaattgaat gcgtttgtcg
    15121 cgcttgaacc attcgagagt aattcccacg agctattgat tccccagtca gaaaaagggc
    15181 cattggctgg tatcccattt cctttaaaaa tgctcggtca agaaaaaaag ggatggctag
    15241 ctacttcagg atctcgttta tttgaaacac accgagcttc tcatacatct aattatgtgc
    15301 ggcaagcaga agcgataggt ctagttcctt ttggacaaac caatgcgcca gagtttggtt
    15361 tcaaaaatat tacagaccca gtcatctatg gcccagcaag aaacccttgg aaccttgaat
    15421 gtacaccggg cggatcaagt ggaggagctg ctgctgcagt ttcatcagga atcgttcctt
    15481 tagctggtgc tagtgatggc ggtggttcta ttagaatacc cgcttcgttt tgcggattga
    15541 ttgggctgaa accttcgcga gggacgatgc cagttggtcc atatgcttgg agaggatggc
    15601 aaggagctgc tattgatttc ggcttgacag tttctatgag agacacggag gctttatttt
    15661 atgggatgag aagtatccat tccggtgcgc catatcaagt atcgccagtt gagtggcaaa
    15721 cacatccaag aaaaaaacgg ttgaagatag ctttttgtgt tgattcaccg atccactcac
    15781 agattagtga agaggcagta cttgctgtaa aaaaagctgt agatttcctt ggaaaagaag
    15841 ggcatgattt gacagagatt ccttacccac tagatggtcg taaactgatc acgtcttatt
    15901 atcagatgaa tggcgcagaa acagcagcaa tgatccgctc gattgaaaca ggcatccagc
    15961 gagctgtcag aaaagaagaa cttgaaccca tttcttggac tttgttccaa tatggtcaga
    16021 agctctctgc cgctacttac atcgattctc tgcatgtatg ggatgaagca gcaattgtca
    16081 tggaaaacct acatcaagaa tacgatctat tcctttcacc aacgactgca tttaccgccc
    16141 ctaaaatcag cgaagatttg caaagtgatg cgataagagc acagatggaa cacatcagtg
    16201 attgttctga agtagaaggt ttagacgttg tttcttccat gtttgaggag agcttgaaga
    16261 tcacacctta tacacagttg gctaatctga ctggacaacc agcaatcagc ttgcctactc
    16321 atttgacaaa agaaaacatg cctctaggaa tccaatttat ggctgccaga ggaagagaag
    16381 atttgctatt tcaaatcggc tatctatttg aagcaaatca tcagttccat ttaccaaatc
    16441 catggaaata gcagaaacaa ggagaagaag aatgaagaaa aaaagattgt ttgctctaat
    16501 aactagtgcc ttttttattt taagcgcttg cggtacagct gccgctaatc aggaagataa
    16561 cagctggagt aatgtagaaa aaaagaaaga attagtagta gcgacttctg gcacactgtt
    16621 tcccgcatct tattataatg atcaaaatga attagttggt tatgatgtag atttggcaaa
    16681 agaggtggct aaacggttaa atgtcaaaat ctcctttaaa gaatacaacg tggatggaca
    16741 aatcacttct gtttcaaaaa gagaatcaga ttttgctgcg aacgattttg gtatcacaaa
    16801 agaaagagaa aaaaaattct ccctttcaga gactttcaaa tattcatttg acagtatgat
    16861 cgtacgaaaa aaagatgatt caggaatcca ctcacttgaa gatcttaaag ggaaaaaagc
    16921 agctggcgaa cccaatacta gctatatgcg tttagcggaa aaacacggag ctaaattagt
    16981 cacatatgac aatgccacga atgatcagta tttaagcgat gtagcaaatg gtcgaacaga
    17041 tgttattcta aatgattatt atctgcagaa aatggccgta tctgctttac cagatatccc
    17101 tgtaaaaata ctggagaacg tttatttcaa tgaaaacgaa agtggttttt tattgaaaga
    17161 aggaaatgat gctctgaaaa agaaaatcga tggcgtatta aaagagatga aagaagatgg
    17221 gacgacaaag aaaatatctg aaaaatattt tcaaactgat gtttcagtca aaccgaaaga
    17281 aacgatggct gagtcggtga gtgagtgatg tactttcccc taattgattt ttccctcatg
    17341 tggacttcac ttcctttcgt actgcaaggc ttggtataca cattagggat tggcttcgtg
    17401 tcgtttcttt taggaaattt ggtagggttg cttttgacag ttttaggtct tttggattgg
    17461 ctgcctctga atgtatttat tcgtttttat ttatcgttct ttagaggcat tcccgcacta
    17521 gtcctgctgt ttctgttata tttcgggtta ccttatcaat taagtgcctt aacggcttca
    17581 gtcatctgct ttacgattac cagcagtgca tttattggtg aaatctatcg aggatcacta
    17641 gcaggcgttt cttcaggtca gtgggacgct ggtttagctt ctggtttttc tttctttggg
    17701 gtattgaaat atatcgtttt gccgcaagct tttcgaatct cagtaccggc actcagtaac
    17761 gtagctatgg atctagtgaa aggtacttcg ttagcagcaa caatcacagt gccagaaata
    17821 tttcaaaaag caaagatcat aggcggtcgg acattcgatt atttttctct ctatatttta
    17881 gttgcactga tttattgggt catttgtatc gggatcgaaa aagtgcaaca gtatttagaa
    17941 aaacgttcta tccatcagtc aatctgaaaa atagaaatgg tgctagcgtg gctggcacca
    18001 tttctatttt ttgtttcttg tatattctgc ctagtatttt aaaaccattt catgtaaaat
    18061 gtaaatcgaa aatatgttcg gaggtagaaa acatgaagac cccgctaacc atcgaaatag
    18121 aatcctcgtt ataccaatat tgcatcgagc aaggcggctt ggttgtagaa gaagtcacga
    18181 tgcctgatga ccaaggaatt gtagatacgc ttagctgctt gacaaaattt gatggaagcc
    18241 gagaatggcg atgctatgag ctgaaagtga caaaagcaga ttttcgctct acagccaaac
    18301 tgtcatttgt tggacattat aattattttg ttttaccaag taaactatat gaagaagtaa
    18361 aaactgaaat ccctacagga ataggtgttt tagtgtatcg tccgtacacg gttgaagagg
    18421 agatgcctgc ctctggtaca tttctgattg cgaaaaaagc agcaaagaaa gagcttgctg
    18481 taccggaaga agctttgaca aaccgcttca tggcttcttt attcagggaa gtaagaaaag
    18541 caaagcaaat ggagcgaggc gtaaaatatt ttccaaaaga tcaattgtac aaagaattga
    18601 aaagacgttc agaaaatcaa gatccatttt ctaacgaacg atattatgaa cggtttttag
    18661 aggaaatagc tagtcaggca atcaatgagt tgcaagaaga agttatcgcg cttcagcatg
    18721 attatgaata tttgaggcaa caacagcaga tccgtcggtt gcctacagag ccattggagt
    18781 gaaaaatatg tactatcctt atttaagagg aaaacaattt gacctattag cattaaaaga
    18841 agcactgtcg cgaggattgc tctccaataa aatccaacca gtgatcgaac cagtacgtga
    18901 ttccgctacg cttaaaaatg tgatcgaatt atttcaaaaa aagcaccatc caatcgctgt
    18961 gatccaaaat ccccaagtag gacaattcaa attattcgat cagcatgtgc acagttggac
    19021 agtcaaagaa aatagctctg tcgtccctgc acagatctta acgccagaaa atctcagtga
    19081 agtactggat tcacctccag cgtttcttgt tttcgatgga cagcactacc caaaagatac
    19141 agaggtgtgg aaacagttgg caggagttga ttcgaaattt ttgataccag atactagtcg
    19201 ctttcgcatc tggctgcccg aaaacaaaat tgtgatccgt gactcttttc aaacgagaaa
    19261 gcatgttgaa agttatgcag ataaaacaga tgactttttt agtgatgact atcttttttt
    19321 ccacgcagac ggatatattg gattttctga tttcacgatc gaaggtagcc gttatttcga
    19381 taaagggttc cctagccgag cgcttgctat ccatctaacg tatattgatg cttacggcaa
    19441 tatacgcgtg aaacattttg tctctgacag taacgactct gctaaagacc aagcattgaa
    19501 gtttcttgaa gcaggagata aaatgaaaaa atggatcatg cgtcaccatc atcaactctt
    19561 gattacttca ggaatgattg aattattggc tctttaccag caacaaaaat tccctggact
    19621 gggagtgttg aaaaagtggt cacttatgca tcatttggaa cttattagtc acctgctaga
    19681 tcatccaaaa gattggctta aatattattc aaaagttcag gaactatatg aagtgatcca
    19741 atgattagat aatgtaaatg aaaaaggaat agaaaagagg aaactggatg tatattcggc
    19801 aaacaaggaa agaagaagta gaagaagtgg tagaaatcat taatgccgca gcggatttca
    19861 ttgcttcgca aggctctcct caatggcaaa acgggcagaa accaacaaaa gaaacgattt
    19921 tgaacgatat tgaaagaaaa gaaagctatg tattaatagt ggaagatcaa attgctggaa
    19981 cagctgcatt agtctcaggc gtggatcccg tttacacaaa aatagatggt agttggaaag
    20041 aatcaactgg tccttattta tcgattcatc gtgtcgcttt agcagaaagt actcgaggaa
    20101 aaaaactagc aaaaccgttc ttagaacatt tgttgacgat tgcagccagc aaaaatatcc
    20161 aagatatccg tatcgatact tatccagcta atgaaccgat ggaaaagacc atctattctg
    20221 ctggattcac ttatcaaggg atgattgaat ttccttttga acacggcgaa agaaaagcat
    20281 atcaaataat catcaattga ctataaaatc atctgagcaa agataaagac attaacacta
    20341 ctgaaaaaaa caatcatatg atagccaaga ggttgtaaca gaagtggcca gcttcaaaaa
    20401 acaagaaaga atttccgaaa atgcttctca aatttttgtg gaattttagc gtattttacg
    20461 aaatagctgc tttctgaaac tcccttcatc gagaggtcgt gacatagctt ttgtcacgat
    20521 ctctcggctt atcctacgag tattattcga tacatttata tcaataatcc tttttttatt
    20581 ttatctgcag cttcttggaa cgctccatcg tttgacacga ctaaaagtga ggggatagct
    20641 ttcaaacgtt tcaactgtgc taaatcttct acataaaccg ccagtctggc tgaaatctgt
    20701 tcagatgaat ctccacgttt tttcaggcgc gattgcaatg tttctttaga gatcgtaata
    20761 aagataggac ggactaccgt cccgaaatac tcatataaat gccaaaaacc atttgctgtc
    20821 aaaacggcgt aacaatttcc tactgtcagt ttctttttaa tttctgcttt actggaacca
    20881 tatttatgtt gagcatattg gtcccactct gcaaactcat ttcccttgat catctgattg
    20941 aatgtattgt cagaaacaaa ataataatcc tcttgatctt tttccccagg tcttggtgat
    21001 cgattcgtgt aagtaatgat cttttgttcc ggctgaaaaa tagcttctgc gagtgaagtt
    21061 tttccagaac cagaaggacc gataaataca tagcaataat tcattgtcat ttctcctttg
    21121 atgattgaac aagtgaaaaa gctttggaaa atatttccaa agcttttaaa agtaactttg
    21181 cttttttgta ttttatacca gcaagccagt cacaatagca cttaaaaagc tgactaaagt
    21241 cgctgttagc aaaattttca atccgtgttt tgcgacaaga tttccttttt cttcatttaa
    21301 tcccttcatc gcaccagaaa tgatcccgat agaagagaag ttggcaaatg aaacgaggaa
    21361 gacagatata attgctgttg tacgctcagt aaaatggaaa ttaccatttg ctaattctgt
    21421 catagcaaca aattcatttg taaccagttt agtcgccatg atactaccgg catccactgc
    21481 ttctttccat gggattccgc tgatgaaggc aagaggggca aagatgtaac ctaggatctc
    21541 ttggaaagtg atgccgaata tcccgtggaa gatagcattg atcatggcaa tcaaagcaac
    21601 aaaaccgata agcatagcag caaccgtgat cgccacatgg aaaccatcaa gaatgtattc
    21661 tcccaacatt tggaaaaatg tctgttcttt gttttcttca atgatcagct catcttcttt
    21721 ttcttctaat acatagggat ttacaattga agccagaata taaccgccaa ataagttcaa
    21781 aacaagggca gtgatcacat atttagaatc gatcagtgcc atataagatc cgacgatcga
    21841 catagaaact gtagacatag ccgatacact catggtgaac aaacgtttct cagataggaa
    21901 gggtaattcc ttttttatgg aaataaaaac ttccgattgt cctaaaatag cagaagctac
    21961 tccattataa gattccaatt tgcccatccc gttgatttta cttaatccta gaccaacagc
    22021 tttcataaat aatggaagaa tcttgatata gcgtagaata ccgataatag cagaaataaa
    22081 tacaattggc atcaatacgg aaagaaagaa aggtgcactt cctttatttg ctacaccggc
    22141 aaaaacaaag tcaacacctt gcccagcaaa agcaagaaga tgatcaaaca tttttgccaa
    22201 tacagaaaca accgctgtac caaaagttgt acgtaataat acgaaaccaa gaacaagctg
    22261 taaaacgaac atcacaatta gtggtttcaa tttgattgct tttttgttac tgctgatcaa
    22321 aaaagcaagc cctaatgtga caaataaacc aataatacct aataaataac tcaaataatc
    22381 actcctattc caaattccgt tttattttac ctgttttgca aataaaatac taatctttag
    22441 tgaaattttc attacttttt tagagtgaaa cgcattcatt ttccattaga aaataaagcg
    22501 aacaaaataa aaggtttaaa aagtgtaacg tcttagtaaa gtctataaaa aaacgaaact
    22561 atcgattaag ctaatagttt cgttttcaac atttataagt aaactttttc tattggtaat
    22621 atagattttc ggaagggtag acaggtgcgc aggtaatatc gattcctagt tttttcaatg
    22681 tttgttcatc atttttattg accatcacag tagagtgggc ttgtgctccc tcaagttcag
    22741 ctagtttatc ataagcaact tgagcgattg gattcgttac tgcactgatt gataaagcga
    22801 tcaagacttc attgatgctt agtgcatcca aagaggaatg aagtgtcttt gatttcaatc
    22861 cttgtattgt ttcaagaact aatggtgaca ataaaggaat gtcatctgcg atatgggcta
    22921 aatgttttac tgcatttaag ataactgccg ctgacgcatc catcaatgaa cttgtacgcc
    22981 cggtaatgat gacttcatct ggtaattcga ttgcaatcac agcagaagga tcttttgtag
    23041 gactttgcgc ttgaatctta tctgcatagt catgggcagg gactaccacg tttcggtctt
    23101 caggacgtaa accgacttct tccataatca gtttgattcg attaactgct tcttcgtcga
    23161 tcaatccttt tttgaagtcg cattctgttt ggaaataacg acggatgatt tcttgtttag
    23221 aggcttcttg gacaacggta tcatccacga tcccaaatcc tacacgattc acacccatgt
    23281 cagtaggaga ttgataaaca gattctttac cagtaatttt ttcaataata cgtttgatta
    23341 caggaaatgt ctcaacatcc cgattatagt tcacagctac tttattgtag gcatcaaagt
    23401 ggaaggaatc gatcatattg acatctttta gatcgactgt ggctgcttca taagcaatgt
    23461 tcagcggatg tttcaacgga acattccata ctgggaaagt ttcgaacttg gagtaaccag
    23521 ctgcatgtcc tttttggctt tcatgataca gttggttcaa gcaagtagcg agctttccac
    23581 ttcctgggcc aggagctgtc actacaacga ttggtttagt tgtttcaata tactcatttt
    23641 ttccaaaacc atcttctgag acgatttttt ctacgttgac tggatagtct tcgatttctg
    23701 agtgtttata gaccttgatg tttcgtcttt ctagtttatt gataaagact ttcgtggcag
    23761 gctgtccact atagcgagtg ataacgacac tgttgattaa taagccataa ccacgtaatt
    23821 catcgattaa ccgcaagata tccatatcat aagtaatccc ataatcacca cggatcttat
    23881 tacgttcaat atctccagca tagacgcaaa tcagtatttc agcttgatct ttcaattttt
    23941 gcagcaattt gattttcgca tcttcttcaa atcctggaag tacacgtttc gcatgcttgt
    24001 cgtcaactaa cttcccacca aattctaaat ataatttgtc atagtggttc actcgttcca
    24061 aaatatacgc tgactgctct tcaatatatt tttctgaatc aaaaccaatt tttttcacta
    24121 ggttccctcc ttaagtacta aagcattttt tcattttttt gaggattcgt caactggaag
    24181 agcgaaattc ctcaaaaaaa caaagaaaat ggtttaaacc aacttgtcta gatagttatt
    24241 cattggtatg atagtttttg ataaggaagg tgctacatga aaaaaagaca gaaaaagaaa
    24301 aacgcatata aacactatat ccgttcgatt tttaccggtt atgaaagaat gcttgaagat
    24361 cccgaattgg aacaattgac gtttacttat ctaaacgaag aaacgcagct aactcgtgat
    24421 gagcatcagc gcattcattt cacaacaaga gatctgccta gtaaataaaa aataagataa
    24481 aggtcatcgg attcgattct gtcctatcag atggaaagac tagttgatcg aaaaaaaggc
    24541 tgtaccaaat tttggtgcag cctgttttat ggagcattgc ccgctctttt ttataacaat
    24601 tttttacaga aaatggtact attgaagtat taataagtga aaaacagatg agtaagagga
    24661 ggcaagtagt atgaaagagc cagatacaaa tgcaaattat gagccagacg aactagagga
    24721 gacgaacgcg gatcaggata caggacaaga atcgtcttct ttttacgata tcaaagaaaa
    24781 agcaagcatg aatctttccc aattattaga agacttacag gcttttgaaa aagctgtccg
    24841 cgaagaagat atccctgaga tctaccgttt atacaatggc cgattgaatg aagaattaaa
    24901 aagaagctca aatgaaaatc acgagatcga tcaattgctg atgcgtaaga tccatgatcg
    24961 ttttttacag gaatttcctt ttatggaaca agtagaaaac atctcgccaa cgatgagcta
    25021 ttacaaaatc ggcacttatt accatgatcg gcccacggtc ggtattgatg caagtctgcc
    25081 agaaattttt gttttgccta agatcgatga agagtgggag aaatattccc aaccggagtc
    25141 aacggactat gacaaaaaaa tcaatgagct agacgcaaag gtgatcactg ctcagacaga
    25201 aattgaacgt ttagatgaac aaataaaaga aatcaatcga caaatgactg atttgaacga
    25261 caataaagga ttcttgaatc gtaaaaaaat cgaggaagaa attcaagaat tagagaagaa
    25321 gaagcaagta ctatctaatg aaaaattagg ctggcttcct tatattgaga cgcctgaaac
    25381 aatccagcag caaaaagaag cattgaagca agaagcaaga gcagaccagc ttcgtgcagc
    25441 catcgtagaa aaagaacaaa ggcaaatcag acgctatttc ggtagcaaag agggattcgg
    25501 tcaagcaatc catgaattct tgatgaatta tcttggcaat gagaatccta ataatcaatc
    25561 taatgaagga ggttccgagt atgaatgaac acgatgaagc agaatggaca aaaaatgaag
    25621 aacaaacaga agatcatgaa gaaacgaaca cttcattgga agaacaaaca agtaatcctc
    25681 tggatgacac agaagaaatc cctatgctag aggaaacaaa aatacatgaa aaagaaaaag
    25741 taactccgac gaatacgaat actgcagaaa acacccatgc ttacgaaata actgaaaatc
    25801 ccgggtattt agaaggaaaa gagcaagatc atcaagatat cgtcagtttg attgaaacag
    25861 cggaagcaga attagcggat ttacgtatga gaaaagattt gatcgaatca gattttccac
    25921 aattattaga atcatatgat cgttttatat tgaatgaaga accaatctcc acgatcggaa
    25981 agacaagcct gatcgaatac ttcacgtatg aattactgaa accgttgaat gatattggtc
    26041 tggaacggtc aaattcagct tatgacacat ggaaaacaag acatttttcg atcaattata
    26101 cgttgaatgg acaaaacgaa ttagtctttt cctttgtatt accgaccatc gatcaacgat
    26161 accaagtgga atcgatgagt ctcctaaacg tatcaccaga gacaatggaa atcaatgtcc
    26221 aagatgaccg ggtgctttct ttgatccgac actggtcagt ggatcgagta ttctctgcag
    26281 ggcagatcac gatattcaat cacaaattga atcaattatt agcccatgca agaacacttg
    26341 gttttaccgt taatcagaca ttactagaca atacgaagcc acttcgtttg aatcttcaga
    26401 gcgaatttga attgacgaat gaagtattgg atgatatttt tatcgtggca atgaaaaatc
    26461 catcctacga tatggaaaag atcaacgaaa ctacttatca agtacgatta gataaaggac
    26521 agtctttaac gataacaaca aacgaaaaag atcaaacagt cttgtcgatc tcatctggag
    26581 actacccacg atctgtcatt gatttcttta tcaattacga attcttggtc ccattaatgg
    26641 tgagaaagtc ctgattggat aagttggatt accagatgtc attcagataa ttctgttcca
    26701 aaggacttgc ggcagttatc cagaataatt gtcacaagtc ttttctgtat gtactaaatg
    26761 gacgtgaata acttgctgat gaaggaggga tttcattagg aatgtccaca aataaaatag
    26821 cacagcttag cttattgtct gctgcttgtg tagcaggtag gatcgctttt caatttattc
    26881 cgaatttcca gccaatgact gctatttttt tatttatagt actttactta agcttaaaag
    26941 atgcgttaat tgtcatgagt ctttctattg cgatcagcag tttttatttt ggtgttggac
    27001 catgggtcat cggccaatgg atcagctata ctgttgttct gagtttgttt tcaatcgtct
    27061 gcttgagaaa aacagtgcaa aggaatctat ggttaaaggg agtctgtttt tttctggcgg
    27121 gcatggttta cggaataggt atgaccgttt ttgatacgct gttatatcgt ttgcctcaac
    27181 cttggatcta ttaccttcaa ggggtgtcgt ttgatttgat gcacagtata ggcaatgttg
    27241 tgttctttct tgtttttctg ccagtcgtca aacgttttta caatcattca ggaggaaaaa
    27301 atgaaaaaaa taatttatag tttgcttgca atgagtgtca gtatactcat tttgacaagt
    27361 tgcagtacaa cctatacaca atcggcttcc aaccaaaagg aggaagtaaa aacatcgcaa
    27421 aaagcaacaa tcacgttaca agaaaatggt caaaactttt cggaaaaaga agtggtcttc
    27481 aaaaaaggcg atgatttact gagtattttg aaacgaaatt ttgaagtcaa agaagataac
    27541 ggcttcatca cttcgcttga agggcactca caggatgaga aaaatcaact ctattggatg
    27601 tttactgtgg acgggaaaag tgctactact ggtgccaaag agatcaaatt gaatgatgga
    27661 caatcggtca ttttcaattt gtctaaacta taagtttcat aaaaaactgt taaagggcga
    27721 atattgccat aattcagtga gttcagttga tgaaagaagg aaaataacat ggaaaatttt
    27781 aaaaaaagcg gaatgttttc cgtaattgct gctctagggg ctaatattct agttgctatc
    27841 agcaaatttg tcggctatgc aatcagtggc agtgccgcca tgctaaacga aagcatccac
    27901 agtatcgttg attgcagcaa tcagattttg ctgttattcg gtgataaaca agccaataaa
    27961 ggacagagcg agttgcatca atttggtgaa ggacgagcaa aatacttttt cagtacaatc
    28021 gtcgcaatga tgcttttctt cggcggtggg gcacttgggg tgatggaagc ttttgaaaag
    28081 ttgacgcatc catcccatga agtgggtaat ccgatcattg ttatagcaat actgttattt
    28141 ggtttggtaa tcgaaggaag ttcgttacgc gtggcaatga aagaaatcaa agaattgaat
    28201 actgaaaaac tttccacgat gcgctttttg cgcgaaagtc gacacagtga aatactgatt
    28261 atttttacgg aagacttgtg tgctgtaatc ggtttggtct tggctttagc aggaacaata
    28321 ttgacttatg caaccgggat tgctgcattt gatgcgatca gcggattgct gatcggtttc
    28381 ttgctgatgg gagccgccat tttcctagca aaagaatttt atagtctgat catcggtgaa
    28441 agcgtgacag caagcgattt atcaaaaatc aagttagctt ttgaacgtcc tgagatcagt
    28501 cggctgatcg atataaaaac agttcattta agtccaacgg agattttagt tgcagcaaaa
    28561 gcagatatcg ttggtaacaa agagaatgaa gcttactcgg tcatcaatga tattgaaaaa
    28621 aatatgcgtc agtctcttcc tgacaagaag atgtatatat acattgaaac agataaatat
    28681 gacccaaatt attctcgtaa ataaaacgtt tattcatgaa ttgaagcaaa aatgagaaaa
    28741 atataatctt gtttttaaat aaaacggcta caatttagcg aatttatgtt acaatgagtc
    28801 cgctgtttat ttgttgtaat taaaggagat attttaatga acaaaaagaa aagtgcgcca
    28861 tggcttcaag ctttgcatgc agtgatgcca ctttgtataa gctatatacc agtaggatta
    28921 gcttgcggag tattgttgca aaaagttgga tttgatcctc tcttatcagg actgttatcg
    28981 attctggtct tttccggtgg cgcgcagttt ttagttgcat ctatgcttac gacgcaagca
    29041 tcatttgcaa cgacattatt gatggttttc tttttagaat tacgctatac attattagga
    29101 gcaagtcttg ccggttttat gaaaaaagaa aagcggacgt ttttggctgt cttttctcaa
    29161 tcactgaatg atgaaaacta tgcggtgaac tacttaaagt tttcaactga cccttcctgg
    29221 aataaaagaa aggctttata tgtcaattgg ttctcaatga ctgcatgggc cggtagcaat
    29281 atgatcggta attttttcgg ctcggtgatt cgagtagatg ctgaccttgt ccattttgct
    29341 ttgacggcca tgttcatttt catgtttatc atgcagatga aaagcgcact actgatcttt
    29401 acaggtttat tctcaggcgt attaggggtc gtattcatgg tattattcca aaatactttc
    29461 ggattgattg cagcaacagt cattgcttca tttgccggat ttatgctgga gaaggcattc
    29521 aaaaaagaaa aagctaagaa actaagagac cgcggtaaag atgtcaatga gccgctcttc
    29581 aacttcccgg aacaggaggc caatcaccat gactaaacta tatttgctac ttttagttgc
    29641 ttgtctgttc atcgtggcat atatcccgcg cttgttcccg atgctttatt ttactcatcg
    29701 aaaagtgcca tcctggttca gtgactggat gaaatatgta ccggtggcat tatttgcggc
    29761 gttagctttt aaagatgttt tcattactca tgaacatctg gatattgctt ggaatatcaa
    29821 aatagctgca atgattctag tagcaggcgt ggcttacaaa acacgttcga tggcgttgtc
    29881 agttttgaca ggacttgcgt ctgtcttttt gctgtctatg ctttaaactg ttttatttta
    29941 ttaaaaacag agattgcgac ataagttgtt caaactgatc tatccgaact gcatttgcaa
    30001 cagtcaattc tggatgcaag tttattcgta gggataaaaa agattagtgt tggcttatga
    30061 tgcaagctct tttttatttt aggaattaga gagggatttg tccttctatg attttttgct
    30121 ataataggga agatgtttat tttatgaaag aatagttgag ggaatttaaa tgaataaaaa
    30181 attgatgcaa ggaacatttt ggctaacctt tgctaatctt ctatgtaaag ttttaggggt
    30241 agtctatttg attccctggc taagtatgat ggggaacaat caagatggta tgcttgctac
    30301 gacattgtat aatgttggct atcttcctta tggattgttc ctgatgcttg gtacagtcgg
    30361 atttccaaat gcgattgcaa aaaaagtagc tgtagccaca aaagaaggag ataacgctgc
    30421 ttgtcggctg atttttagaa gtacgatcaa tatcatgttt gtcatcggta tcgcctctgc
    30481 tgcactgatg tatttatttg ctcctttact agccggaatc agtccgattg ctaacgttaa
    30541 taatgggata ctagctatca gaagtttatg cccatctttg attgctatcc caattcttag
    30601 tgcaatgaga ggatatttcc aaggaaaaaa tgatctgcgg ccttatggaa cttctttgat
    30661 tatcgaacag gctgtgcgag tgatcgttat cttggctggt acttattact tgcgcgtatt
    30721 gactgacgga accatattag aagctgtatt gatcagtaca gtcgcttctt tcttcggtgg
    30781 gttagctgcg attatccata tgtatatcgt cggacgcaga aaagacttct tcgaacttaa
    30841 agattttttg atttccagcc gttatttcga aagagaaaac cgatctgcct ctgtctctat
    30901 cattcgggaa acactaccgt ttatttttgt cggctcagtc attactatcg ttcaaatgat
    30961 cgaccaagta acgatgaaac cactgcttca ctttttccgt ccagagatcg cagatcagca
    31021 actggaattc ttattcagcc gtgcttctgt caatccaaat aaattgacat tgatcctgat
    31081 ctccatggta ggaacagtag ctataagcag cttgccaata ttaagcacga tgaagaaaaa
    31141 agatcggata caaatcgaaa aaacagtagg agatagtttt tcgattgcgt tattgattct
    31201 tcttccttca ttgactggaa tgtctttatt agctggacca ctatacacat tgttttttgg
    31261 ctatgatcct gaaagtgtcg gatatttcca aatggctttg ctagcttctt tgtttttctc
    31321 tttattcacg atcctatcta cgatgcttca atctttgaat caccatcgtt ttgcgatccg
    31381 attaactgta gaagcaatta tcctgaaagt catcttccaa gtaatcggat taggattagt
    31441 tggcggatac gggatgagct tatcaaatgg cttagcattc ggtattgtct ttgttcgcgg
    31501 ctatcacttt atgtgcaaag aataccgaat agcaccttta gcaaagatca gcaatttttt
    31561 cttaaaaact ttccgctcca ccctgattat gcttgcagta tgttttgccg ttttcttttt
    31621 gctgaatcag cgattgtcaa tggaatcaaa aagctatgca atgatttatt gtgtggctgt
    31681 aggaagtatt ggcggcttag tatttttatt ttctcaattc ggaaaaaatg ggtttcgaat
    31741 gttgaaaaat gttaaacatc atagcagaag caaagaatag gttaactaat cgttttttgc
    31801 tgtcacttga aaaacggcaa ctcgtatgat tacgcttgtc gtttttctat tttccaattt
    31861 tagtttcata tctaatctta cttatgatat agttataagg tctggaaaaa ttctttgaaa
    31921 cgaggaaatt aaacacatga aacttgcagt agttacagat agttcggcat atctgccgga
    31981 acgtatcaag aaccatcccg acttattcgt tattccaatc cctttgatta tggatgggaa
    32041 aatttataac gaaggaatcg atatcgaagc ggatgaatat tatggacttt taaaaaacag
    32101 taaagatttt cctactactt cacaacctgt cgtaggggaa gttttgggtt tatatgagga
    32161 attgaaagag aaagggtatg acacgatttt aagtatccat ctttcttcag ggatttctgg
    32221 ctttgtcaac acattatttg cgatgaaaga tgacatcagc ggagtaacgg tcattcctta
    32281 tgactctaag atcactagta tgccgatggg acacatggta gaagctgcct tagacttaaa
    32341 tgatgaaggt cagtcccttg atgaaatact tgcacatatc gatcgaatcc gagataatac
    32401 ctatgcttat ctgattgtag atgatttgaa caatttagta cgaggaggac gtttgacaaa
    32461 tggtgcagcg ctgattggca gcttattaaa aatcaaaccg atcctgacat tttctgaagg
    32521 gaaaattgtt ttattcgaaa aaatccgttc aagtaaaaaa gcatttgctc gagctgaaga
    32581 aattattggt agaagaaata acgaaataca accgccagtc aaactttacg tgatacatgc
    32641 aaataattta gaagtagcga ttgcggagaa agagaaattg cagaaaaaat atccaaatgc
    32701 agaaatcgaa attggtcatt tcggaccagt cattggtaca catttaggtg aaaaagccat
    32761 cggcattgcc atttcagcgc aataattcct tttattcaaa aaacatacca aaaata
//
LOCUS       DAZKOW010000017        32497 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000017 DAZKOW010000000
VERSION     DAZKOW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.017"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACG912_000854"
     CDS             complement(<1..563)
                     /locus_tag="ACG912_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFQ0512195.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="ACG912_000855"
     CDS             complement(750..1400)
                     /locus_tag="ACG912_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512196.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG912_000856"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="ACG912_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFQ0512197.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG912_000857"
     CDS             complement(2912..3910)
                     /gene="yvcK"
                     /locus_tag="ACG912_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFQ0512198.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG912_000858"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="ACG912_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFQ0512199.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="ACG912_000859"
     CDS             complement(4926..5657)
                     /locus_tag="ACG912_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFQ0512200.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="ACG912_000860"
     CDS             complement(5661..6926)
                     /locus_tag="ACG912_000860"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFQ0512201.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG912_000861"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="ACG912_000861"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFQ0512202.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG912_000862"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="ACG912_000862"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFQ0512203.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="ACG912_000863"
     CDS             12268..13770
                     /locus_tag="ACG912_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFQ0512204.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="ACG912_000864"
     CDS             13772..14509
                     /locus_tag="ACG912_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512205.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14680..15873)
                     /locus_tag="ACG912_000865"
     CDS             complement(14680..15873)
                     /locus_tag="ACG912_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0512206.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(15952..16158)
                     /locus_tag="ACG912_000866"
     CDS             complement(15952..16158)
                     /locus_tag="ACG912_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFQ0512207.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(16151..17293)
                     /locus_tag="ACG912_000867"
     CDS             complement(16151..17293)
                     /locus_tag="ACG912_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0512208.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            17805..18440
                     /locus_tag="ACG912_000868"
     CDS             17805..18440
                     /locus_tag="ACG912_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0512209.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            18594..18671
                     /locus_tag="ACG912_000869"
                     /pseudo
     CDS             18594..18671
                     /locus_tag="ACG912_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            18985..19032
                     /locus_tag="ACG912_000870"
                     /pseudo
     CDS             18985..19032
                     /locus_tag="ACG912_000870"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG912_000871"
     CDS             19048..20967
                     /gene="tet(M)"
                     /locus_tag="ACG912_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0512210.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            21086..21253
                     /locus_tag="ACG912_000872"
     CDS             21086..21253
                     /locus_tag="ACG912_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0512211.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(21313..21666)
                     /locus_tag="ACG912_000873"
     CDS             complement(21313..21666)
                     /locus_tag="ACG912_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512212.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            22170..22589
                     /locus_tag="ACG912_000874"
     CDS             22170..22589
                     /locus_tag="ACG912_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0512213.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            22615..22821
                     /locus_tag="ACG912_000875"
     CDS             22615..22821
                     /locus_tag="ACG912_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512214.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23049..23222)
                     /locus_tag="ACG912_000876"
     CDS             complement(23049..23222)
                     /locus_tag="ACG912_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512215.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            23234..23863
                     /locus_tag="ACG912_000877"
     CDS             23234..23863
                     /locus_tag="ACG912_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512216.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACG912_000878"
     CDS             complement(23945..25111)
                     /gene="dnaJ"
                     /locus_tag="ACG912_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFQ0512217.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACG912_000879"
     CDS             complement(25262..27091)
                     /gene="dnaK"
                     /locus_tag="ACG912_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFQ0512218.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACG912_000880"
     CDS             complement(27142..27705)
                     /gene="grpE"
                     /locus_tag="ACG912_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFQ0512219.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACG912_000881"
     CDS             complement(27799..28842)
                     /gene="hrcA"
                     /locus_tag="ACG912_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFQ0512220.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACG912_000882"
                     /pseudo
     CDS             complement(29076..30241)
                     /gene="hemW"
                     /locus_tag="ACG912_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
     gene            complement(30257..30484)
                     /locus_tag="ACG912_000883"
     CDS             complement(30257..30484)
                     /locus_tag="ACG912_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFQ0512221.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            30968..31575
                     /locus_tag="ACG912_000884"
                     /pseudo
     CDS             30968..31575
                     /locus_tag="ACG912_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            31748..32461
                     /locus_tag="ACG912_000885"
     CDS             31748..32461
                     /locus_tag="ACG912_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512222.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
BASE COUNT         9520 a         6618 c         5736 g        10623 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc tctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaaa ataaattata
    14521 tatgtctttt tgactatctt tttatatctt cttaaatgtc cgcaaagcct tattttatag
    14581 gctctacggg cattttgctt ttgtggtaaa cctcacatat ctaggtctat cttcttatat
    14641 tttcactatc aagcgtggtt aaaatcgtgg taaatacttc tatgcaatta gacgctgaac
    14701 ctctgatttt gcggtatcta tggacgcatg agcataccag ttcattgtga tactgatatt
    14761 tgaatgcccc atgatatact gtaaatcttt tgggttcatg ttcttgcttg ccagtcttgt
    14821 gcagaatgta tggcgtagcg tatgcggtgt gatatgtggc aatggattgt ccttgtggtg
    14881 cttgttgtat ttctttacca tacggataaa taaggcgtta taatcaatcg caactttggg
    14941 cttgccctta tgattgacaa ataggaagtt gccccgtccg tctatcacaa atggttctgc
    15001 ctttggacgt tttttcataa cctgttgaaa tgcctgtatc gtttctttgc ttaatggtac
    15061 ttgccttatt ccgctttttg ttttaggcgt ttcaatataa tagccctgtt ccttgctctt
    15121 tagtaactgg tggtcgataa tcacaacttc attcttgaaa tcaatgtcgg ctactgtcag
    15181 tccgcacagt tccgaaatac gaagtcctgt ctttaacagt atcagcacat catcataatg
    15241 cttgtgatac acattgtccg tcttgatgaa tgagagtaag gcttgttcct gttcctctgt
    15301 caatgcgact ttctttttgg tatcattttc taggacttca ctcaacttga aatcaaaggg
    15361 atttttcctc acataatcat cttgaatggc tatgtagaat gacgctttta acgagcgttt
    15421 atggttgtta atggtattgt aggaaaagcc tttgtctttc atgcgtaacg cccattcctt
    15481 agcgtcagag ggtttgattg tatcaatgct cctagcacct aacttgtcct ctttcaataa
    15541 ccgcatgagt tgttcccgtt gtttctgtgt gcttttcttc acatttgccc tctgtgcgtt
    15601 ctgtttggca taaagttggc aaagcgtcat tttcttgcca gtgctgtcga taccgtcctc
    15661 aatatcccgt cttatctgct gttccagttc tcgaagtgag ggtttttccc gttttccctt
    15721 tggtgtcggg tctgtgggtg tcaatctcca agcatacaca tattttgtgt ttccaaatgc
    15781 gtctacatat ttatataagt attttctgtc tgttcgttgg ctctctccag tatgcaggat
    15841 acgacctttg ttatcccgtc ttttttcttt catggtgtct gctcctttcc ttgttggaaa
    15901 gagccttgat atgacttgtg ttcatcataa cacatacaag gctcatttgc attagatagc
    15961 gtccaattta tcaatgacct gttcaaactg tcggcgttta atctgtatgc ggttgccatt
    16021 cataatgagc cagccagcgt ccttgttttc ctctgccaat cgtcttaact tgttttctcc
    16081 gatacggaaa tactttgacg cttcttcaat ggtaagggtg tatttttccc atacgggaat
    16141 atcctgtgca ttactcataa aacaccccct cactttcgat tacgtctgct atgtccgtac
    16201 acatctgttc cacaagcatt tcctgctttt cagagaggtg ggtacattca tcaatcattt
    16261 tcatgtagtt tgagcctgtc agcctgtcga tttcctctat catcacgatt gacggcatga
    16321 cctgtctttc cagccattgc agagtgcgtt ccatattgac aggtacgggg ttcatttcaa
    16381 aaggtaaact gtcatgggaa atgaatatct cccacagcgg atagtcggga aattgcacaa
    16441 aatcagtaat aaggtaaaac accagcccat gagggttata tgtcaataac aattcctcaa
    16501 ctgcctgtac tgcttttgta ccacgcagac gaatttcaaa acgattgata atatccgtat
    16561 gtttcttttt ggttgcctgt tccttttgtt tttcatacag gcagaagtat tttgtgcttg
    16621 cctttgaacc gatataaagg gtgcttgcta aattacggtt ttttccgctt agttttccac
    16681 cctgcacatt ttcataattt ttacagcgac aatccgcacc gccatttctg tatttttcag
    16741 acaaaacggg aatatccaac agaccacaca tatcattgat tgccaagtca aaacgtcgga
    16801 taacaccgtc acagtcaaga cagcggttta aaaatgaata ccaatctctg ttctgtgcct
    16861 gcaagacata ttccatgttg cgtgagcctg ccccttttaa ttccaagaat acgcccatgt
    16921 gttcgtcatc agaagccatg accttgattt ctccgtaggc gtaatgctct ttatagccat
    16981 aatacccata gtcataatgg ataaaataat ctgctttcat gccaagcacc tttcggataa
    17041 tttccaatgc gtcagtcgtt ggataacgga cagacagata atcaacgagc agataaaaag
    17101 gcttatcgca gatataataa ttcagacaac gcaggataat attttgtgtt tccttgctgg
    17161 cgttggcttt tccgttctca aagcggttga tactatatct tgatacatgg gtaagacttg
    17221 caagttctcc ttgtgaaaga ccgcttgcaa gacgtttgtt cttcatttca agtgcaaatt
    17281 ctaaattgtt cattgtattc ctccaatccc acaatagcct tgaaatacct gtaaacctct
    17341 attcaaaaag tgtgatatgc actatcttct taaaacttct cattactact cacaactttg
    17401 ggattgtatg gtttttgact tgtcttttgt gaaatagtgc ccccctgtta gataacgggg
    17461 gcacagacaa ggctcgcagg ctcgccagcc gacaaagcat ttcttcccct tgacacaagg
    17521 tgtcatggcg gtaagcctga gaggcttccg ccaccgtgtc agaaatactt tgtcagcaag
    17581 ttctgttttg ccttttctaa caagttccat ttcaaccgat attcatatct acggatttaa
    17641 tcagataggt atttcttttt gaatgttttt atatttcatt ctattgtgct tattcgatta
    17701 caagaacata gtaattctta tatgcttatt tgtcaatatc tttttttgcc tttacaaatc
    17761 atattaagca tgatagactt ataatagcga agatataacg agatatggag ggttatgcaa
    17821 tgaaaataag ccatttagga aataatatac agaccataag aaaatttagg ggaatgaaac
    17881 aacaggaact tgcggacaaa atcggtatca atatgcagag cctttccaag attgaaagag
    17941 ggttgaacta tcctgcctat gaaacgctgg aaaagataat ggaagtgctg gacgttacgc
    18001 ccaatgaatt gttgtcggga gaatggaagt atgtcaatca atctgaaaag gaagtctgtc
    18061 agtttttaag agccgaagaa cgtctaaatg cagaactgaa acatggacac tatgataact
    18121 tctttgacag cgaggaagaa tggctggaat atgagttaga aaagttacgg gaatatatta
    18181 cagactatat caacgggaaa agcattgaag cgtccgacct ttacccaatc aaagaattta
    18241 tccagcattt gaagtttcaa aaattattag accgctatga tgatttatac agtatggata
    18301 tgtttgggga aagcatagag ggacataagt ataagacacc ttatcaagtc gtgaaaatga
    18361 taaacccgaa ctcaaaagaa gatatggaac tgttacgaga agtattaagg aataacaatt
    18421 ttgatgatga ggacgagtaa cttttttcct cttgcgagaa gatacagcaa acaaaaagcc
    18481 cgatcaagag tgcaaagcac caccaatggt ggcaaagctc ttgacagggc tttttctttt
    18541 ctgttgtggg caatcaagag gaagaaaggg ataaaatctg atttgtggta aatcaggcaa
    18601 aggcaacgca ggtatctcaa tttgatttgg tacttgaaaa gaacgggagt aattggaaga
    18661 ttatagaata acaaatattg gtacattatt acagctattt tgtaatcacg tactctcttt
    18721 gataaaaaat tggagattcc tttacaaata tgctcttacg tgctattatt taagtatcta
    18781 tttaaaagga gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag
    18841 tgggaacaaa taattggatg tcctttttta ggagggctta gttttttgta cccagtttaa
    18901 gaataccttt atcatgtgat tctaaagtat ccggagaata tctgtatgct ttgtatgcct
    18961 atggttatgc ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt
    19021 ttgggttttt gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat
    19081 gttgatgcag gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca
    19141 gaattaggaa gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag
    19201 agaggaatta caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac
    19261 atcatagaca cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt
    19321 ttagatgggg caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata
    19381 ttatttcatg cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac
    19441 caaaatggaa ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa
    19501 attgtaatca aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa
    19561 tctgaacaat gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc
    19621 ggtaaatcat tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt
    19681 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
    19741 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
    19801 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatat acgcctttat
    19861 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
    19921 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
    19981 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
    20041 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
    20101 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
    20161 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
    20221 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
    20281 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
    20341 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
    20401 cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa
    20461 tcgtttcaaa atgcagttat ggaggggata cgctatggct gtgaacaagg attgtatggt
    20521 tggaatgtga cggactgtaa aatctgtttt aagtatggct tatactatag ccctgttagt
    20581 accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt aaaaaaagct
    20641 ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca ggaatatctt
    20701 tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac tcaattgaaa
    20761 aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga atatcgtagt
    20821 gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa agggtaccat
    20881 gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat agataaagta
    20941 cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa tatggtttct
    21001 tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa aggaggagat
    21061 agttattata aactacaagt ggatattgtg tcctgtatgt ggaaataaaa cacgattaaa
    21121 gataagggaa gatactgaat taaaaaaatt ccccctctat tgtccgaaat gcagacaaga
    21181 aaatttaatt gaaataaagc agttcaaagt aactgtgatt acagagccag acgcaaagac
    21241 gcagagccga taaaatgaga ttaatacaat ctcattttat cggctctttc cgttatgtat
    21301 ggattctttt aattagtctt cgatgtttct tgcttcgttg ataccgctgg ctaaagattc
    21361 cattaaggat agttctttgt ctgtaaagct atccatgtat ttctctatct gtaatcgtcg
    21421 ggtgcttttt accaagttat tagcaggtaa gaaaaattca tcaacggaaa catgaagtaa
    21481 cgatacaagg tcataaagaa cttgtatgct ggggtgttgc cctttatttt caatattagt
    21541 taagtaccgt gggtcaattt caatcaatgc tcccacttgt tcacgagtta aacctcgttt
    21601 caatcgagct tctttaatgg ctaaaccaaa ggctctaaaa tcatatttat cttctttttt
    21661 acgcatagta gaccacctct atacatttta ttgttcctac tgaattaaaa acaggtatag
    21721 aaaaacgtgt tatatggttt ataggtttat atttaataaa aagcactact aaacgccaat
    21781 aaaaaaaacc gttatatggt agtgctattt acgctgttaa aatattgtat attacttcca
    21841 aatggcggtt tgttggaggt caacgtcgcc atgaagtaca tcatatacaa taaatttcct
    21901 tacattgggt tcttgtcaaa aaaagtcgtc tatctgcaat agataagtac gtccaccaat
    21961 gtggttttat aaatcatata gatagaataa cagaagcatg taaacagaga aataaatctg
    22021 tttatatgct tttttggcta ttcagaactt ttttacaaag tttatttatc agtaatgcaa
    22081 caaatccccc tttcacattg ggactaagag tgaaaggaga taaacgagca aggctcactt
    22141 cctttcctag acagaaaggg ggtgagatta tgaggacatc ttctttcgag catatcgtca
    22201 gattacagtt caattcttta atgctgattg tcattaagaa caaagtgaaa agtcgaaaca
    22261 gacagcttgc aagacattct aaacgtgaag tattgttttg tgaaatagcc gaaacgaaac
    22321 aagttgaacg tggaacgatt gatacctgtt tttatgattg tgtatcattt aaagtcttgc
    22381 actttacaat ttatgtatct gatgaaacac ttggtacagt tttacataga ctatcagaaa
    22441 aacagcgtag tgcgatttta ttacgttatt ttcaaggaat gaacgacaga gaaatttcag
    22501 aactgtacca tgtatcacgt tctgctattt gcagtagaag aactagagga ctaaggaagt
    22561 taaaaatgct tttgagcgaa aggaagtaaa gattatggct atccctcttt tgaattgatt
    22621 tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt ttatgacggt
    22681 tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa aatcattatg
    22741 caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc aatattgaaa
    22801 tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca cagtttcgtg
    22861 ttatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg taaattcgtg
    22921 gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt atgcggataa
    22981 ttgaaaatta gatataaaat ttaataaaaa ccaaaaatgc acggtcgcca tgaagggacc
    23041 gtgtattttt atcttcttat tttctcacct aatcgtatca gccgcatcgc ttgttttcct
    23101 gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat cggttcattg
    23161 atgatatcaa gtacatagtc gataccaccc tcacttaatt gagcttgatt tttcttctcc
    23221 atattatgta gttgtgggac aactttctga caatcagcaa ttgtgtcaag aacggatcaa
    23281 tcctttattg gagttgctcg aaagggtctt ctcttattac ggacaggctt tgctcactgt
    23341 acatagacaa caaatcatta tcctagtcaa tcggatttcc agagcctcat tgctatcatt
    23401 gcttgataaa atccagacaa agtttaataa aatgtatcag ttacagctaa actttggaat
    23461 tgggtcgctt tgttatacag agcaagagac accgcaatct ttccttcatg ccaaacaagt
    23521 ttgcgaatgg atagctttcc atcaatctgt gaacgagatc cgcttttttg aagatttaga
    23581 cctcgggatc gttttaccag caatcccgtc agatcaacga acattatatg taaaacgtat
    23641 tttgaaaagc ctgacagaag aagaagtaca tttgttcaag aagactttgg catgcttcag
    23701 taaaaataac ggaagcatca aaaattgctc agaagaatta tttattcata aaaacacctt
    23761 gcaatatcga ttgaacaagt tccattctct cactggttat acccctagaa actatgacga
    23821 ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    23881 ggcgaacata tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    23941 aaaactactt ccgctttttc ccgccaaaag catctttaaa cttatcgaag aaaccgtctt
    24001 cttgctgttc ttcaactttg atgccgccag cttcagcgaa tgcacgtaat gcttctcttt
    24061 gttgttcatt tagatttttc ggtgtgatca gttttacttt gacatgttgg tcgcctgttg
    24121 caccaccacg taacttcgga gcgccttttc cacgtaaacg gaagttcgta cctgtctgtg
    24181 ttccagcagg gatcttcaat ttcacatctc catggacagt tggtacttgg acttcatccc
    24241 ccaaagctgc ttgtacaaaa cttaaaggca attcatagta aatttcagaa ccttcacgat
    24301 caaagatatc actttcttct acacggaaga ccacatacaa atcaccaaat ggtccgccgt
    24361 tttctccagc ttctccttga ccagctaaac gcatttgttg tccttcttct acaccagctg
    24421 gtacattcac tttaactgaa tgtgctttct tctcatgtcc tgagccatga caggttggac
    24481 aaggttcttt gatttctttc cctgtcccat gacaaacatc acaagtttga cggctcatca
    24541 ctcgtccaag tggtgtttgg cgttctacat taatcgtacc tgcaccatga catttatgac
    24601 aagtagttgg ttgtgtgcct ggttttgcac cgttcccacc acaagtatga cagatttctt
    24661 cccgattata cttgatttct ttttctacac caaaaatcgc ttcttcaaaa gacaagtcga
    24721 ccgtatattg caaatcagca ccttgacgag gcgcatttgg atcgactgac cgaccgcctc
    24781 ctccaaagaa agagtcgaag atatcttcaa aaccaccgaa gccgccacca gagaagccgc
    24841 cgaatccacc aaatccgcca ccagctccac cagcaccgta atttggatct gtgcctgcat
    24901 gcccgtattg gtcatatgca gcgcgttttt gcgggtcact taaaatctca tatgcttctg
    24961 atacttcttt gaatttttct tccgcatcag gttctttatt gatgtcagga tggtattttt
    25021 tcgaaagctt acgataagct ttctttattt catcttctga tgctcctttt gagagcccta
    25081 agacttcata ataatcacgt tttgttgcca taggctttcc tcctattggt tctcatcatt
    25141 ttcacgtcgc ttgttatcat atcatatttt gtttgaaaca aaaaccgata cttcaaacta
    25201 tttttcaaaa gaaaaagcca aagtagcaac tttggccttc tctcgaaatt cagtgaactg
    25261 attatttatc gtcgccatcg acttcttcaa aatcagcatc aacgacatca tctgctccgc
    25321 cttgagctgc ttctgggttt tcttgagctt gttgttgtgc cgcttgttcg tatagtttaa
    25381 cagtcaagtt ttgaacgatt tcgttcaatg catcgcgttt agttttcatt tcttcgatgt
    25441 tgtttgcttc aacagcggct ttcaattcat cgcgagctgt ttctgctttt ttcacttctt
    25501 cttcgtccac tttgccttct aattctttca atgttttgtc cacagagaat agcaatgcat
    25561 ctacatcatt acgtaggtca acttcctctt tacgagcttt atctgcttct gcatttgctt
    25621 ctgcatcttt taccatacgt tcgatttctt cgtctgttag acctgaagaa gatttgatcg
    25681 tgattttttg ttctttttgt gtacctagat ctttcgcaga tacatttaca atcccgtttt
    25741 tatcaatatc gaatgttact tcgatttgtg gaataccacg aggagctgct ggaatatctg
    25801 tcaattggaa tcttcctaga gttttgttgt cagcagccat tgggcgttca ccttgtaata
    25861 catggatgtc tactgctggt tgattatctg ccgcagttga gaatacttgt gatttacttg
    25921 ttggaatcgt tgtattgcgg tcaatcaatt tagtgaacac tccacccatt gtttcgatac
    25981 ctaatgacaa tggtgtaacg tcaagtaaaa caacatcttt cacatcacca gtgatcacgc
    26041 caccttggat agcagcaccc atagcaacta cttcatcagg gttaactgat ttgtttggtt
    26101 cttttcctgt ttctttacgt actgcttcta caactgcagg gatacgagta gatccaccga
    26161 ctaaaataac ttcgtcgatt tctgattgtg ataaacctgc atcttttagc gcttgacgta
    26221 ctggtacttt tgtacgttct actaagtcag ctgttaattc atcaaatttt gcacgtgtca
    26281 atgtcatttc caaatgcaat ggtccagatt ctcctgctgt gataaatggc aagctgattt
    26341 gtgtgcttga tacgccagat aaatcttttt tcgctttttc agcagcgtct ttcaagcgtt
    26401 gtaaagccat tttgtcttga gacaagtcaa tgctgttttc ttttttgaat tctgctacca
    26461 tgtagtcgat gattttgtta tcaaagtcgt ccccacctag atggttgtcg ccagctgttg
    26521 acaatacatc aaagacaccg tctcctagtt caaggataga tacgtcaaaa gtaccgccac
    26581 caaggtcgaa tactaagatt ttttcgtcgc gatctgtttt atctaaacca taggcaagag
    26641 cagctgcagt tggttcattg acgatacgtt ctacttctag accagcgatc ttaccagcat
    26701 cttttgttgc ttgacgttgt gcatcattaa agtaagctgg cacagtgata actgcttttt
    26761 cgactttttc acctaaatag tcttctgcga atcctttgat atattgaagg atcattgctg
    26821 agatttcttg tggtgtatat gatttacctt ctacatctac tttgtaacct gcttcaccga
    26881 tatggcgttt gattgatgaa atcgtatttg ggtttgtcac agcttgacgt tttgcaactt
    26941 cccctacttg gatttctcca tttttgaatg atacaacaga aggtgttgta cggtttcctt
    27001 ctgggttagc gatgattttt gcttcgtttc cttccaatac tgctactgca gagttagtcg
    27061 tacctaagtc aataccgata attttactca taattaacgt ctccttcttt aattgaaagt
    27121 gtaagtttta tatccgcaca attattgtgc aacaatgacc atggttggtc ttaacacgcg
    27181 atcatgtaat ttgtagcctt cttgtagtac ttctacaatc gtatcggctg gtgtatcttc
    27241 agtcgctgga acagtttgta ctgcttgatg aagattagga tcaaaggctt ctcctttagc
    27301 agggattttt tcaattcctt cttcttccag tgcgttacgc aaactttcca agaccatttc
    27361 cacgcctttt ttcaaacctg ccccttgatc atctgacact tctgtggcta atgcacgttc
    27421 caggttgtca attgatggaa gtaatttttt ggctaaatct tgtgaacggt atttttgtaa
    27481 ttgctcacgc tcattttttc ctcgatttgc catatttgcg atttcagcac gagcccgcaa
    27541 gtatttatct tccatttctt ctaactcagc ttttaatttc gctgtttcga attcttccgc
    27601 ttcagcttct gaaatacccg aatcttctgc agcttcgaca tcgatttccg gttctggctg
    27661 agcatctgtc atttctttat ctagttcttc ttgattttct ttcacgatac agtcttcctt
    27721 tcattattca gtcagctgtt tgattgctct tactaagaaa gagagaacga tcagctgcaa
    27781 atagcttcgt tattcacatt acgagtttga caagtcaagc gaacggtagt aatcagcaag
    27841 tttagctgct agttctcttc gataaacatc gactagaccg aacatccgag aataaggcat
    27901 actagtaggc cctaacaagg ctattgtacc tcttccatga ccatctatct cgtaacttgc
    27961 ttgaatcata ctcatattct gcaataaatc attgcctaat tcagcaccaa tcttgatatg
    28021 aatattgcta tccatcggag tgatcatttg tgtaagtccg tctgagtctt tcataaaaga
    28081 aaacatcgat ttgaactgat caagatcttg acggggctca taatccaaga gattcatttg
    28141 tccactgaca taaaccttat cttcaaaaac atgaccaaga acggtgtcaa ataactgaga
    28201 catcccttct gtcgtttgga aatatttatg aagaatcatc ggaatctccg tacgaagttt
    28261 atggtacacc gtcatcagag gatctccgac tagacggtcg tttacaatct gtaccatttt
    28321 ttctagttcc tgacttccca tatattcggg aagagtgaac acttggcttt ctacattccc
    28381 catatctgtt acgacgatag caataatctg tcgattattc aagggtacga tacgaaagcc
    28441 agtcagtcgg cggtctttta tttcaggtcc caatgaaaaa gtcgtatagc ttgtcagctc
    28501 agataatatc tcagcagatt gcttaataat ctcattgatt tcatgaaatt ctctgcctag
    28561 agactgacga atcagcagca aatcatcttc tgctacgcga gcaggtttta acaaatgatc
    28621 cacgtaataa cgatagccta aagcagatgg aatccgccca gaagaagagt gagtcttcaa
    28681 cagcaatcct tcatcttcta atgctttcat atcattgcga atcgttgctg agctggcctt
    28741 gactccctca gacatcaatg ttttggaacc aacgggcacg ccactactcg tatagtgttg
    28801 gatgatcaga cgtaaaatgt cgttttgtcg ctttgttaac atcttcctca cctctcatta
    28861 gcactcatat attctaagtg ctaatccact tataaatata gcaagaagcc cttctcctgt
    28921 caagagaaag actccatttt ttagcactct aaacacaaga gtgctaattc taaataattg
    28981 atgtaaatcc agttatatca acgtttcaat agtcctttat tgtatttttg aacttttttt
    29041 atttaaattc agataaaaaa caaatattct ttaaactatt tttcaaacaa aaagcgctca
    29101 aacacattgt tccccaaaaa ggtccctctt ggtgtcaaat aaacacggtc ttcttcatta
    29161 acaagtaacc cttcttgctc taattccctt agcacatcac cgtacaccga ttcaattgtt
    29221 ttttgatagc gatcatagaa atgtttttta gtaatccctg tttttttctt agtcctaaga
    29281 acatttcttc ttctatttga ttttttcgtg tcaatttttc ttcttccaat accgggcgtt
    29341 catttgctcg taatgggcgt aaatagtgtt ggattggtcc tttgttgcgg taacgaatat
    29401 ctccaagata accgctagca ccagctccaa acccaaaata gtggtcattg ttccagtaca
    29461 tgaggttatg ttgagattca tgtccggaga gcccaaagtt acttatttca tattggtgac
    29521 gtccatgctt ttccattgct tcaatcgtct cttcaaacat tctggtttcc gtttcctgat
    29581 caggcagttc gagtcttcct tgtcgtaccc agttcatgaa cattgttttg ttttcgagga
    29641 tcaatgagta caaagaatag tgaggcagat ccaaagccaa agctttttcc aaagtatcca
    29701 gatatccttc tagtgtttgt cccggtaatg cataaatcaa atcgatgctt acattggtaa
    29761 aattttctgc ttctaaaaat tgcatagttt gatacacatc ttctgcggtg tgtttacgtc
    29821 caattttttt caataagcga ttatcaaatg tctgcactcc catggataaa cgattgacgc
    29881 catagttttt cattacttga agtttttcac gggtcaaatc acctggattc gcttctacag
    29941 taaactcatt accattttga aaaggcagga tctctctagc acctgataac agtcgatcaa
    30001 gttgtttagc agataaagaa gttggtgtcc cgccccctac gtaaagtgtt tctaaattcg
    30061 ctgtcggatg actttctagc attattttca tttcattcag cagcatttcc acatactcat
    30121 ctactggctg cccttctaaa aaaactttat taaaatcaca gtaatagcag atatgctcgc
    30181 aaaatgggat atgaatgtac gcagaaacct tgttttctgc gggatttatt gtttctatca
    30241 ttgaaattct ttctccttac gtcgaaaagt cgtgcctgtt ttcgaccttc ttaaaatcgc
    30301 gttcaagcac caactcattg aaataagccg catatttaga atcaaggaaa cctagctcta
    30361 aaaatgcttg cttattgtca tcggagctac acggtgcttt tacagcctct cgataaacgg
    30421 taatcgcaaa gctgaccttc ggcattaagt caaaatctgc aaaaatatga aaagctattt
    30481 tcacagattc ctccttaagt gctcagacca caacgctttg cttacaacct ctcatatacg
    30541 catactaaac gtatttggcg aacaaatcca ctaaaaaatc caagaatcga cttcttggat
    30601 gattttttct ttcattaaac tggttaccta gttatatatc tctagggttg ttttcatgtc
    30661 tgagtgcccg atatgattta tttttcgttt ctataattac tttttgcctt ccagtttctt
    30721 ttaatattgt actcaagcaa gtgggaattg gttcttttgt catccatgta tgcaaaatgt
    30781 tataatgctg gtaaacaaaa atgattacct gtcattttta tttagttcgt atttaattaa
    30841 gttgactatc tgtatactct gtctaaaact taagaattgt tgttttcgct gtcgctaccc
    30901 aattttaatg aatgtctgta ggttaaaact gttgtccttg ttatagatag taaaggagag
    30961 cttgaaaatg aaaactgcga ttacgtttct attagaggaa tatgccgatt gggaaggtgc
    31021 ttatattact agcacactaa accaaaatga gaaatggcaa aacaaaactg cttctatcca
    31081 atcagaggtc acttccattg gaggattaaa aacaaaaatc gattacttaa tcgaacaaat
    31141 acctgaaaaa gttgaccttt ttattatgat cggtggcaat tcatgggaca taaaaaatca
    31201 tcatttatat caaataatcg ataaattgtt aaaaacagac gtaccagtag ctgctatttg
    31261 tggggcagtt gattatttag ctcagagcgg attattgcaa ggttacaaac acaccggaaa
    31321 tgcgcaattt ccttggttag aatcggaaaa ttatcagaat aaagaagatt ttatagaaca
    31381 acaagctgtg aaagatcgta atttgattac tgcgaatgga acagctccta tagaattcac
    31441 agacttgatt cttcaagcag tccattttga aacagaagaa ataagagaaa aaacgattcg
    31501 tctatacaaa cttggattct atgattacta taaaaagtac ggagatcctt tctcctaatc
    31561 attctcttca attaaatact ccttatcaat agtgtgacaa gtaattttac tgatgattat
    31621 ttgtcacact ttttctgaca tttatcaaag aagatacact ctatttatat tcatagacaa
    31681 ggttttacgg attcaaaaat tcaggattat gctatactgg tattaaaaaa ataatgaggt
    31741 attttatatg aaaaagttag gttttatttt attgttggtc tctttattcc ttgtaggatg
    31801 tacgtctcct agtaaagaaa atagtgatcc atctgcgacc acagacaaaa gttcaatcag
    31861 acaaaccagt tcagctgaaa aatcaagtac tcagaaatca gcttctactg aaacgaatac
    31921 atcttcaaca agcagtaaag tctcagagcc caatacttct tctacatcta gtagtacagt
    31981 tgagacaaat atttcttctt caacaataga cacaacctcc tcacagtctt ctcagtcaat
    32041 agatccgtta gcaggatatt ctgatctaca aattgagtac gcacgtgttt ggctagctat
    32101 tagaggtaat acttttagag atacatcggg tgatttcgaa ttacatgtta ttcattatcc
    32161 tgcaggaaca cctatcgccc cttatgatgc agggagtgca gtctatccga aagatgtggt
    32221 tatgctcact gggaaataca gttaccaggg agttttagtg tattcaagta accatgacgg
    32281 gacaatcact agttatccag ttccttcaca ttggcaaatg cctgcagatc aaacatctta
    32341 tccagcgttc attagagaaa caactcaaga aatcattgat aatgcatctg tagtagctat
    32401 tcctacagga aatcccaatg acgtgaaaca gttaatcgat gtgacagtta tagataatta
    32461 gactttctaa tatgttatac aaaaagaagc tgacagc
//
LOCUS       DAZKOW010000018        31955 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000018 DAZKOW010000000
VERSION     DAZKOW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.018"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1412
                     /locus_tag="ACG912_000886"
     CDS             <1..1412
                     /locus_tag="ACG912_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512223.1"
                     /translation="EKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLNDINNIK
                     KEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQL
                     TECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSW
                     ELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEE
                     IKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQV
                     FIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEE
                     QTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDV
                     HYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENI
                     IPISINTIDRAIISAVQNK"
     gene            complement(1504..1869)
                     /locus_tag="ACG912_000887"
     CDS             complement(1504..1869)
                     /locus_tag="ACG912_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0512224.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1866..2231)
                     /locus_tag="ACG912_000888"
     CDS             complement(1866..2231)
                     /locus_tag="ACG912_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFQ0512225.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2423..2671)
                     /locus_tag="ACG912_000889"
     CDS             complement(2423..2671)
                     /locus_tag="ACG912_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512226.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2670..2798
                     /locus_tag="ACG912_000890"
     CDS             2670..2798
                     /locus_tag="ACG912_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFQ0512227.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2855..4549
                     /locus_tag="ACG912_000891"
     CDS             2855..4549
                     /locus_tag="ACG912_000891"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFQ0512228.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4649..4822
                     /locus_tag="ACG912_000892"
     CDS             4649..4822
                     /locus_tag="ACG912_000892"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512229.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5200..6333
                     /locus_tag="ACG912_000893"
     CDS             5200..6333
                     /locus_tag="ACG912_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFQ0512230.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6364..6684
                     /locus_tag="ACG912_000894"
     CDS             6364..6684
                     /locus_tag="ACG912_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFQ0512231.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6713..7177)
                     /locus_tag="ACG912_000895"
     CDS             complement(6713..7177)
                     /locus_tag="ACG912_000895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0512232.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7309..8562)
                     /locus_tag="ACG912_000896"
     CDS             complement(7309..8562)
                     /locus_tag="ACG912_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0512233.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8591..8908)
                     /locus_tag="ACG912_000897"
     CDS             complement(8591..8908)
                     /locus_tag="ACG912_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFQ0512234.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8934..9254)
                     /locus_tag="ACG912_000898"
     CDS             complement(8934..9254)
                     /locus_tag="ACG912_000898"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0512235.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9718..11133)
                     /locus_tag="ACG912_000899"
     CDS             complement(9718..11133)
                     /locus_tag="ACG912_000899"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0512236.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11150..12466)
                     /locus_tag="ACG912_000900"
     CDS             complement(11150..12466)
                     /locus_tag="ACG912_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0512237.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12616..13314)
                     /locus_tag="ACG912_000901"
     CDS             complement(12616..13314)
                     /locus_tag="ACG912_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFQ0512238.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13603..14592)
                     /locus_tag="ACG912_000902"
     CDS             complement(13603..14592)
                     /locus_tag="ACG912_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFQ0512239.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14672..15505)
                     /gene="msrA"
                     /locus_tag="ACG912_000903"
     CDS             complement(14672..15505)
                     /gene="msrA"
                     /locus_tag="ACG912_000903"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0512240.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15680..17314)
                     /locus_tag="ACG912_000904"
     CDS             complement(15680..17314)
                     /locus_tag="ACG912_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFQ0512241.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17301..18989)
                     /locus_tag="ACG912_000905"
     CDS             complement(17301..18989)
                     /locus_tag="ACG912_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512242.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            19135..20772
                     /locus_tag="ACG912_000906"
     CDS             19135..20772
                     /locus_tag="ACG912_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512243.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(20917..21657)
                     /gene="truA"
                     /locus_tag="ACG912_000907"
     CDS             complement(20917..21657)
                     /gene="truA"
                     /locus_tag="ACG912_000907"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0512244.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(21848..22483)
                     /locus_tag="ACG912_000908"
     CDS             complement(21848..22483)
                     /locus_tag="ACG912_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFQ0512245.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(22630..23850)
                     /gene="sstT"
                     /locus_tag="ACG912_000909"
     CDS             complement(22630..23850)
                     /gene="sstT"
                     /locus_tag="ACG912_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFQ0512246.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            24069..25067
                     /locus_tag="ACG912_000910"
     CDS             24069..25067
                     /locus_tag="ACG912_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512247.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            25263..27098
                     /locus_tag="ACG912_000911"
     CDS             25263..27098
                     /locus_tag="ACG912_000911"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0512248.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(27158..27874)
                     /locus_tag="ACG912_000912"
     CDS             complement(27158..27874)
                     /locus_tag="ACG912_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFQ0512249.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(28101..28643)
                     /locus_tag="ACG912_000913"
     CDS             complement(28101..28643)
                     /locus_tag="ACG912_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFQ0512250.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(28648..28875)
                     /locus_tag="ACG912_000914"
     CDS             complement(28648..28875)
                     /locus_tag="ACG912_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFQ0512251.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(28954..30834)
                     /locus_tag="ACG912_000915"
     CDS             complement(28954..30834)
                     /locus_tag="ACG912_000915"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0512252.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(31023..31637)
                     /locus_tag="ACG912_000916"
     CDS             complement(31023..31637)
                     /locus_tag="ACG912_000916"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0512253.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            31779..>31955
                     /locus_tag="ACG912_000917"
     CDS             31779..>31955
                     /locus_tag="ACG912_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0512254.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYD"
BASE COUNT        10065 a         6251 c         5216 g        10423 t
ORIGIN      
        1 ttgagaaaaa tattttaaga cagctgtttt tatgtgggca attctatgta aataaagaag
       61 tcaatctaga aaagctctcc aatctcctgc atgtttgcaa aaccacttta ctcaatgata
      121 tcaataacat aaaaaaagaa tttgaagaac agatcgctta tacacatcgg gaaaaggacc
      181 gttacacttt atatttttca gaacatatac ctcgatgcaa gatcatgcag caattgtctc
      241 aaaattcgtt gtttttaaaa acttgtctgc tttatttgga agaagatgaa cctgattatc
      301 ttcaattaac tgaatgtgaa ttcatctctg tcagcaaagc ttattcatta aaaaaacaag
      361 tattagctta ttttaatgac tgcgggatcg aaatcgaccg ctactcccct cgattcactg
      421 aaatggaaag acgtctgctg cttcttaatg tgtcgtatcg tttaggcggt tttaattctt
      481 gggagctacc tgaatcgttt tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa
      541 atagtggcag attttatgat aaagaaaata aagaaatctt atcgattggt tttgctatca
      601 gtttcctacg ccagcaagtg tgtgctgtga caatagattc aaaatttatc gaagaaatca
      661 aaaaacgtcc tatttacaat tatgtcgaat cagcatggga aaacactgat ttccaaacct
      721 attataaaaa agaagaattc gcctttattt tgacactatt caatctttgt aattatggtt
      781 tccattctta tcagttgatt gcggaagact ttcaacagct acaccaagtg tttattgaca
      841 atactcccga aataaaagaa ctagttgcta cctttgaatc tcacttcaat caagaattat
      901 tcggaaacca accgttcgaa cgtgcgctga ttcatctgat gcgttcggct tgggataact
      961 atcaattatt catgccagaa aaattttatc ttttaaatga ggaacagaca aatttatata
     1021 aagaagtcca gacaattttt tccagctggt ccagccagtt accttatgat ctacggttga
     1081 atcctaattg catgcgggct tttgtgatcg aattgagcgg aattctccgt ctgacaaaag
     1141 aacatctgac tatctatatc gtcacaaatt cggatgttca ttatctgatt tatcgggaag
     1201 ccttggaagc tgtaactact ttcgattttc aagttgcacc aacaatttat tcaagtatat
     1261 ctgatataaa gaagtatgcg cagcagtcct caaaccgggt attatgtgaa cgtacattat
     1321 atacaccaga tgcggtccaa tatgaaaata ttattcctat ttcgatcaat acgatcgatc
     1381 gagctatcat ttcagctgtg caaaacaaat aaggggctgt gacaaaaatc atgtcacagc
     1441 ctcttatttg ttgaagatgc ccatttctgt aacaatctct tgatacacgt caggctcaca
     1501 acctcactta ttttggacat ctacgggtcc gatctcttta tttaaaaaat aagaaatgat
     1561 ggttcggata actaccaacg ccgctagttg gaaaatatcc tgcaaggtag gtttgataat
     1621 cgattcgatg atcccagctg aaacaagaat ttccaaactt agaagtatat agcctcccaa
     1681 catcttttta atagcattgt tctcgctgac tcggtcgctt tgcacttttg tatggacaaa
     1741 taaatatttc aaaaaatcct tgattgatag acaaacaccc caaaccaata aacagatagc
     1801 aaagatatca agtcccagta caagtttttc gaaatatgga agaagaaact gcatcaactc
     1861 tgtcgtcatc ctacctctcc tcttctttgt ttttctttgt cttctatttc ctgattcaag
     1921 aaataagaaa tgactgttcg aatgataata ataagtgcaa gcttcaaaat atctgtccaa
     1981 gtcggcttga taactgactc gatgatatca gcaacgacta gtacttccaa gcttaacaaa
     2041 atataacttg ctagcagttt tcttgtatct gcattacgag tattacgttc tgcatagttt
     2101 ttcttgacat gagaccaatc aaaaagtttg aatagaacaa gaacgatccc gacaattaag
     2161 ataccgatag aacaaatatt taaaatagca atgatccatt ctagaaattc aattaaattt
     2221 gagcgaaaca tatacgctac ctactttctt ttaatttgtt cctattcttt tattgtaaaa
     2281 gaaagttacc ttgttcgtct atttaaaagt tgttctaatg ataacggttc gcttaaattt
     2341 ttctttttca ctctcccatt tttctccctc catccataaa tcgtggaagt atccaaaaca
     2401 acgtagcaag ttaaaataag gcttagtccg tcatcagctt ttcaaagaag cactgtctcg
     2461 gtgctttttt tgtacccaaa tttctaaaaa tagaaccata cacctcctca aatcttttct
     2521 tcttacctac cctattttta tttacaggaa aatcagacac ttcttctttt ttgtcaaaat
     2581 ttcctttttc ttctcgtcct atcatttggc attctaataa aaacgaacta tccttggata
     2641 tagaaagaga aaacaaggag gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg
     2701 gtttaggttt gcttgccctt ggcgctgcat atctaggaaa aaagacagga ctattcgaag
     2761 atgacagtca tttatatgat gaattcgaat caatctagca tcctatttag ttgaatcact
     2821 acggacatac ttggaagaca ttggaggaat cacaatgaga aaaagtaaag caataatgat
     2881 tggtgccggt attgccaata tggcagcggc tgtttatttg attcaagaag gcaaatggaa
     2941 aggggatcag atcacctttt attcattgga tgatcacggt tcaaatgatg gtgcaccgac
     3001 cgacactgta gtggatgaat attggaataa aaaccatcca ttagaaaacc aaaaaggcta
     3061 tattgctcga ggcggtcgca tgcttaatta ccgtacctat gtagatttga tggatctgtt
     3121 aagtcgaata ccttctgtta ctgagccagg gatgactgct gaagaagata cgagagattt
     3181 tgatgcaaaa caccgaacct ttgacaaagc acgtttgatg gaaggtggca aaggaatcat
     3241 tgatggcgga aaactgggat tcaataataa agaccgtatt cttctgacta aactgattgc
     3301 tatgcctgat tcagaagaag aaaaactaga caatatcact atcgaagaat atttcaaaga
     3361 tgatccacac ttttttgaaa caaacttctg gtttatgtgg gaaacaacat ttgctttcag
     3421 aacacgtagt tctgctcaag aattgcgtcg ctatatgcat caaatgattt atgaattcac
     3481 tcaaatcgaa catctagtgg gcgtcaatcg aacacgttac aatcaatacg aaagtattat
     3541 gcttcctttg atcgagtact tgaaagaaca aggatgcaaa atcattttga accgccgtgt
     3601 cactgactgg gaattcaaag aaacaccgat gcaagatgaa atcactgtga ctggtttgaa
     3661 aattaccaat acactgactc aagaagaaga atacgttgtg gtcgatgaag atacagcagt
     3721 tattttcact aatgggtcca ttacagattc agctacttta ggagattacg aaacaccagc
     3781 tgtagaaaat atggattatg gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt
     3841 ctacaacttg ggaaatcctg acaaattctt tgctgatcga gatgcaagcg aatgggtcag
     3901 ctttaccctg acgacgaaaa accatttgtt gttgaatgaa atcacccgta tcacgactca
     3961 agaaccagga aatgccttga attcatttat ttcaaccaca ccgatcacgc cgttaggcca
     4021 aaaagatgtc aatatgtcta ttgtcgtgca tcatcaaccg cattttacgt ctcagaaacc
     4081 aaatgaaaca gttatttggg gttatttcct ttatccaaga cgacgtggcg aatttgtgga
     4141 taaacagtat attaaaatga atggaaaaga aatgctagaa gaactgattg gacaactttc
     4201 aaaagtagat cctggaccag tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt
     4261 aataaataat atccctgtct atatgcctta tgcttccgcg ttgttcaata atcgtgccaa
     4321 aagcgatcga ccaaaagtca ttcctaaaca ttcgaccaat ctggccttta caggagaatt
     4381 tgtcgagcag ccttatcaga tgatctttac tgaacaaagt gctgtacgtt ctggagaaat
     4441 cgctgctttc cattttgcgg gtattcctat gtcccgtctt gtcaaaacac cgcgttacga
     4501 caaagatatt cctactctag ctcgtgccgc aaaaaaaatg tttgaataat caagaggttg
     4561 tgaaaaagct gccctgcatc aagtaataag aacaaccagg ctaaaacagc ttcttatgtt
     4621 tttgatttgg ttggacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg
     4681 ttatgaaaaa gctgccctgc atcaagtaat aagagcagcc aagctaaaac agcttcttat
     4741 gtttttgact tggttagact tattaccgca gatgcaagct tttgaacacc gtttaccaag
     4801 aggttatgaa aaaggcgttt agtttcgagt atgaagaaaa attttgtgaa aatggttcct
     4861 cacgtttttg cataaatttg acttaatacc gagaaactga tcccttctgt cataacctct
     4921 ttttatttat gtctgtttct ttttgaaaac aactattttt tcaacgttta ctctcttgcc
     4981 aatccaacat catctaacgg atacaactat ttcaatgatt accattacac taaaaaatcg
     5041 gaaacacacc tctccgaaca caaatacata agactataga atgtttcatt taagaaaaaa
     5101 agcgtttcat tatcaaaaag gtaagatctc ctttaaaatg aaattatctg tagtactaat
     5161 aatgcagaca attttatgaa tctgaaagga gcatctccaa tgaaagcagt cacttggcaa
     5221 ggaaaacaga aaatggaaat cagaaacgta gatgatccaa aaatattaga accaacagat
     5281 gcaatcatcc gtatcacagc aacagccatt tgcggctccg atctgcatct ttaccatcat
     5341 ggtgaagcag ttttggaaaa agggtatgtt gtaggacacg aaccaatggg aatcgttgaa
     5401 gaagtgggat ctcaagttca aacattgaaa aaagggcaac gtgtggttat cccttttaat
     5461 attagttgtg gtcattgtca tttctgcctg aatcacatgg aaagccaatg tgatgaatca
     5521 aatccagatc agttatatgg cgggcttttc ggatttggca agttgaacgg taatcattgg
     5581 ggtggtcaag cggaatatct aagagttcct tatgcagact ctacttcatt tgttgtacca
     5641 gataatgatc ttcctgatga aaaagtttta tttctatcag atattttgcc cacagcttat
     5701 tggagcgttg aaaacgcagg agtaaaaaaa ggcgatacag ttgtgattct aggttctggt
     5761 ccagtcggtc tattcgctca aaaattcgct gttatggctg gagcagagcg cgtaatcgca
     5821 gtagatccag tacagcaccg tctagataaa gcggctaatt acaatggggt tgaaacttat
     5881 ttcttagaag acacggcaca agctggaaca gaattgtatg aactggctaa aggtggcgca
     5941 gatgtcatta tcgattgtgt aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa
     6001 aatttagtaa gcttacaaag cggcacaatc tctccattac agatggcatc gcaagctgtc
     6061 aaaaaatttg gaacagtcca agtaactggt gtttatatga ctccagcttc ttcttacccg
     6121 ctgcaagaat tttttatgcg ggatatcgct gtcaagcatg gacaggcacc aattatccat
     6181 ctaatgccaa aaatttacgc aatgattgca gaaggactat tcgatcctac acaaatcatt
     6241 actcattcgc tgccactatc agaagcagca caagcatatg agatctttga caaaaaagaa
     6301 gataaaaata taaaagttgt tttgaaacct tgaattcagt aaaaaataca ggtgattttc
     6361 tttatgccca aaatcaaacg aaatacccat gagatcgaaa taggaaaaga agaagatatc
     6421 gaaatacgag gacgtcgctt ccgcttctat ttccaaaatc gttacacatt gatctcactg
     6481 gctgtcgatt tattaaccgg gattttctat atttttggaa gccttgcttc cttaactaaa
     6541 atccccgatc gatatggcac ctatttttat ttggctggtg ctattttctt gacgattcgt
     6601 cctattctca gaatcgtaag gaacattttt atttacgacg aaaaaaaagc aatgaagaga
     6661 acaagcaaaa aaaaagaggc atgagaattt ttcaaatctc atgcctcttt tttcacccta
     6721 tcgtatcact ttgatacaga cgagcggtcg tcccactttc ttccttgacc attgtcaaaa
     6781 atgtccactc gtatcgttca taaaacgtgg tatgatctgt aattaagtaa agtttttcgt
     6841 acccaagttg atgcatttct tttctgattt ccgctaatat ccgcttggct atcccctgct
     6901 tacgcttttc cggttcaaca aacagggcac aaagatttgg tgtcagatca gggcggttat
     6961 ggaagtcgtt ttcaatgacg cccgctcctc cgacgatggt ttctttttca tctaagacca
     7021 cataccattg aggaatactt tccttattag cgattccagt acgaatactt tcctcataca
     7081 caacagcagg aactccccat tttgatgaaa accaattcgc gctttgtttt actaattctg
     7141 ggtgttctct taatttgatc cagttatatg taaccaatgt ttaaacctcc gactaaaata
     7201 aaacgtttac aaaaattata caagtcttta tgaaaattag caatgacaac aatagtgatg
     7261 taaaaaaaaa aacggaaaac aatcacttgt tctccgtttt ttgattaatc aagcatctaa
     7321 agcttctttt cttctttctt ggatcgtagc taccttgacg aacggtagat agatcaaaat
     7381 agaaagaacc aaattgacag cggctagtac accacctgca atactttttg ttgccaatac
     7441 accgccaata attggtggag ttacccatgg aggcataacg gtagctgctg gcactaaacc
     7501 tgtagaagtc gcaaagtaag caacactaac taagaccatt ggtgtcaaaa taaatggaat
     7561 gaacatgatc gggttcaaca cgataggtaa cccaaacatc gttggttcat taatattgaa
     7621 gacacctggc gcaatcgaca agtttgttac aaccatatat ggtttgtttt tacgtcctac
     7681 taaataaata gctaacagta aacctaaagt tgcacctgtt cctcctaagt tgacaaagct
     7741 gtcaaagaaa ggtttgttca cgataaacgg tgcgctatgt ccagctgcga ttgcattaac
     7801 gtttgcttca atggctggcg cattgattgt ttgcatcaaa ggatcgacca tattcgctcc
     7861 gtgtaaacca aagaaccata agaatggtgt gataaatgca agcagtaagg cagatggata
     7921 agagtttgca agtcccataa atggttcttg gaccactgta tagaaagaac ctacgatatc
     7981 atcaacaccg aatgcagcaa aaattgcagc aactaaagca aacgcagaga tagtgatcat
     8041 tgctggtagt aatgaagcaa atgcttttgc aactgccggt ggcacaccat caggcatttt
     8101 gatgatcaat ttttcatttc cgcttaaacg tacaaagatt tctgttgaaa taatcccaac
     8161 aataatagca ataaacaaac cgtttgagct catacctaat gctccgccta aagcgaagaa
     8221 agagcctaaa gaaatcacac ctgcggaaac accgtctttt cggtagccgt tagctaaatg
     8281 ataagcaagc aagaaagcaa tcaagacaga aagaatggca aatgtaccgt tccaagcagc
     8341 agccccgaat cctttccatg attcaccacc aaaaattgag ttcatcaatt cttggaaccc
     8401 gggaatcggt aagttattga tcattactgc taatgctcct aagatcatta acggcatact
     8461 caccataaat gagtcccgaa tcgcaactaa atgccgttgt gcaccaattt ttgatgctac
     8521 aggtataaaa tgtttctcca taaatgcaac aaatttatcc ataaatcttc cctcttttct
     8581 ttactgtaga ttacgcgttc aatcgacgat acagatcaat gatctctttt gctaaatctg
     8641 taaaagctat agatgtcatc agatgatctt gactgtggac cgtcaaaagc gtgacttcaa
     8701 cgtgatttcc ttgtgcttct tgtgtcagca tctctgtttg agagtgatgt gcttggacta
     8761 aagattcctc tgcatctttg attttttcat ctgctaaagt gaaatcacct tttttcgcag
     8821 cagcaatcgc ttccattgca tcgctttttg catttccgcc aaacatgatc agacccatga
     8881 tggcttctag atttttttga tcttccattt attgtatcct ccgataattt ttattatttg
     8941 tttattagtt tttctgcttg gtcgagtact ttttcaccat tcatcatgcc atagtcagcc
     9001 atattgatta catctaaagg aatgccttta ggtgctagct tttcagcaaa ttgattttgc
     9061 ataaagcgta cttgcggtcc taaaagtaaa acgtctacat ttttagattc tagttgatta
     9121 tccgcatctg aagcagatac agcaaagatg tctgcttcca ttccttttgc ttctgctgcc
     9181 ttttgcattt ttgtgactag gagacttgta ctcattccag ctgaacatac aagcatgatt
     9241 gttttctttt ccattttaaa cacgttcctt tctatactta cacttgtata taagcaagta
     9301 gcgtgccaac tttaaaagcg ctttaaaaag ttgatatcat cacatttaag gtctatacca
     9361 agttgcatac acactaaaaa agaaagcgca cactaaacac acaaattaat ttttattacc
     9421 ttttttcttt taacgtgcac aaaaaaagtt gtgacacaaa acttgtcaca acttttttca
     9481 aataaacggt attcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacata
     9541 agatgttgtt tttactttgc tattcttatt acttgatgca ggacagcttt ttcacaacct
     9601 ctcaataaac ggtgttcaat cagctgaccc tgggtattag ctcaaaacgc tggattcaca
     9661 acctctttta tttcaaataa ccagtaaata caatgaacat ctacaaaaaa cttctattta
     9721 tttatagatt tcgtcaggca aatccaattc tctcttatca atcaaagtct gataccacga
     9781 agcagatttt ttcaatgttc tgcttcgttc atcgttcaat tcgattcgaa ctaatccata
     9841 tcgattctta aatgcattca ttggtgacac acagtcagtg aatgcccata acatatagcc
     9901 ttcgcaattt gcgccttctt ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa
     9961 ttcgatacgg taatcatctt gcacgattcc tttctcatcc atatactgtt cttcatattc
    10021 tcttcccata ccgttttctg ttatcagcca agggatatta tgataatttt cttttagata
    10081 catggctata tcatacatga ttttcggata gatttcccat cccctggaat tattcatttt
    10141 cttcccaggt aaatcaaaga cttcataata tcgttcagga tggaaaggtg tctcttcatt
    10201 ccattgataa cgaggggcgc gaacgcgttt aggataatat tgattgattc caaggaaatc
    10261 tactgtatga tgctgaatcg tttctaaatc ctctgttgca ggatcgaaat aaatcgattg
    10321 tttttgacac aactccagga actcttctgt gtagtatcct tttaccatcg gatcaaaaaa
    10381 tactcgatta taaaacaaat catacatata agcagctttt tggtcttctg gagaattgga
    10441 tcgagcatag accatttcag gatttaaaat acagccaatc cttccaccat aatttccgct
    10501 gcggtaaatc tctactgctt tagccgttgc taaaacttta tgataattcc acatcatcca
    10561 cttttttgtg ttttgttcat gcggccagcg aacagcatcc aaatagcaac gcgtttgggg
    10621 aacgattggt tcgttgaatg taaaccagtt ttttacccta tcaccgtaac gatcaaaggc
    10681 aatttgagca tacttagcat aaaaatcaac tacttttctt gaagaccagc catcatattt
    10741 ttccaaaaga aaagctggga cttcataatg ttccaaacaa atcatcggtt ctacgtctgc
    10801 ttcgatcaaa gagtcgatca tttcacttat atgctgagca tattcttcat ctactataca
    10861 gttctcataa tcagtaaaaa aacgagccca attgatagaa gtccgataat gtgtcagatg
    10921 gatggatttc atcagatcca catcctgctg gtattgttcc ataaaattag ttgctacggc
    10981 tgggccatac ccattatgcc aaacaaaatg ttcattttta taccagctat ctataaacga
    11041 atcctgtcct tctttttttc ctttccaacc ttccgtttgc caagcagaag atgcagcacc
    11101 aagaataaaa ttttttggaa tagtaatttt catgatttcc tcctgttttt tagatcccat
    11161 cattttccac acgatttgct acttttacaa atggcagata gatcaaaata gaaacaataa
    11221 tacaaacaag ttgcgtaata actgcaccga tatttcctgc tgttgccaga taagcattga
    11281 cgatcggcgg cattggccaa gggaccatta caactgcttt cgccgcaaat cctaaagcgg
    11341 ttgcaaaata accaatcgtt ccagtgataa gaggcgttaa gataaacgga atagctaaaa
    11401 ttggattcag catgatcggc ataccaaaaa taactggttc attgatgttg aagatcccag
    11461 gagcaaggga taacttggtg atttgtttca tatcttcccg ttttccaact aacaatactg
    11521 caatcagtaa accgatcgtg actccagacc cgcccatact catatacata tcccaaaatg
    11581 gcatagtaat aatgttcggt acttcttttc cagcttcaaa agcttccgta tttacagcaa
    11641 ttgccgctaa aagcaacggt tcccgtatcg gcttcaccat ttgatttcca tggataccaa
    11701 tcacccaaaa gacttgcgca ataaacatca atagcaaaat acccggtaac ccttgtacga
    11761 caccttctaa tgggcgttgt acgacattgt aaatgatatc ataaaggtac attcctgtaa
    11821 gttctttgat gataaatccc gcagccgcga tacccgatac agtcagaatc gtcgggaata
    11881 aagcagaaaa actagcagaa acattcgttg gcacggtatc aggcattctc agctgtagtt
    11941 tcttctgttt cctcaaccag caataaagtt caatagaaag gatggagacc aacattccca
    12001 aaaataaccc tttcgtatct gtatattgtt tagccaatac gttttctact ataacagtct
    12061 gattttcatt cactaattca taaacagttg gattaacaga taaataagac atcactgcga
    12121 tgataccagg gaatacacca tccactttat tcagcttggc taattcgatc cctatcagaa
    12181 aaacagcata gatggtcaag aaacttagtg tgacatagct gatggcagat gcgataggct
    12241 ttaattcttc taaaaaacgc aagaaactta tctgtgctaa gccattttcc gagtcaaaga
    12301 ccattgctga aaacaatgtg ccaaaagcac ccgtaataat taccggaatc aatgcagcaa
    12361 atgcgttttt tatcaccatg atatacctta aattattgat tctcgttgcc acgttcccta
    12421 aaacattggc taatttatcc ataaaactca ttccagatcc cctcattcct gcttcttctc
    12481 cttgacgtca tctctttttg tctattagtc agcgcttaca tttcgatgaa cagaaactat
    12541 ttactcctct tttttaccaa atataaaaca aaaattcatc tgatgtattt atcataaaat
    12601 acatcagatg aactgtcaat tagtttcttt ttctcctatt attttctgta attctctccg
    12661 atttattgcc atatggacag tcattgcatc aaacgcagca agtgcattcc gcctttcgat
    12721 ggcctgaacg atctctgtat gaatatcgat cgtctcgatt ttcagatgct ggatatcata
    12781 atttttatta aacaaagcaa ttgattcatt aatgattggg ataatatgca gcaaggctac
    12841 gtttccgcta gcttctgcaa tcatcgtatg aaaatcgatg tctaattgaa catgttcttg
    12901 tgtgccttct cgaaaagaag tttcaatctt ttgcgttaat tttttcattt ggacgatttg
    12961 attcttcgtt gcatattccg ctgccaaacc tgccatctta ggctccaata aatatctcag
    13021 ttcaaataag tctcgtatca tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc
    13081 attggcaatc cctcgcttat cactgacaaa tgtccccgct ccttgccgaa cttcaaggat
    13141 attcctggaa gctagagccc gcacagcttc tcgcaccgtg cttcgcccca cctctaaata
    13201 atcggacaat tcatattcat tcggcagttt atctcctact tgcaactgca tttcctttaa
    13261 atatctaagg atcttgtcag tagttacttc tactagggtt ttattttctt ccaaaccaca
    13321 tacctccttt tcatctgatg tattttttcg taattattat aacacgtttg aatcatctga
    13381 tgattttttg tatacaaaat tgattaatcc gttcatagac atacaaacga agaagatatt
    13441 taccataaaa aagactagga taaaattcac tcctagcctt cgatcatttt tattttgaac
    13501 gacaaaaaat atgatgttca ataacagacg atcttcattc gattcttgat gtcatctttt
    13561 gtttttttta atatatggat aaaaagtttg attacatcgt ttctattcta gaatttggtc
    13621 gattctcaat tcttcttctg gtgagaaatc taaattatcc atcattcgaa cactttcttg
    13681 taactgactt aaccgacttg ctccgaccag tacacttgta acagattttt gtcgcaaatt
    13741 ccatgcaagg gccatttgcg ctaatgattg tcctcgtgat acggcgattg tttgcagtgc
    13801 tttgattttt tccagagttg atccaacttg ttcttctgaa aggaatggta tttccttacg
    13861 atgtgctcta gaatcttcag gaataccatg tagataacga tttgtcagca acccttgagc
    13921 aagcggactg aacgtgattg cccctagccc ttcttcttct aatactggag atagatcgtc
    13981 ttctagtagt gcacgagaaa acatattgta gcgcatctga tgaataatga acggtgcttc
    14041 ctttcttttt aaaatttcag tcattttttt tgtatcttct ccattatagt tggatatacc
    14101 aatatataat gcttttcctt gtcttacaag ttgcatcaaa gcttctgctg tttcttccaa
    14161 aggtgtatca gggtctgggc gatgatgata gaagatgtca acataatcta gccccattct
    14221 ttggagactt tggtcacaac ttgaaatgat ggatttacga gagccccatt ccccatatgg
    14281 cccttcccac atatgataac ctgctttgct agaaataatc agttcatcac ggtaggcttg
    14341 aaaatcttct ctaaaaatac gcccaaagtt tttctctgca gctccagcag ggcctccata
    14401 attattcgca aggtcgaaat gggtaattcc catgtcgaat gcgcctctta aaatctctct
    14461 ttgattatgg ataggatcat aatcaccaaa attttgccat aatccgagag ataaaatcgg
    14521 taatttcaaa cctgaatgac cggatttacg atattccatt ttttcataac gtgcactatc
    14581 tgcaatatac aatttttctc ctcctcatca aatcttttta aaacgctttc ttttttctat
    14641 ttttagttta ctacatttta aagaaaagga gttaatcaaa atgatttttt cgtatatttt
    14701 tttgccattt gttttttact cgttgataga tcagaaattg attacgtgct tgctggattt
    14761 ttttataacg tttcggttgt tttttgtaaa attgctgatg atagttttct gctggccaaa
    14821 acgtaccggc tggttcgatt tttgtgacga ttggttggtg gaaagttcct gaactgtcta
    14881 gtttctgttt tgattgttcc gctaattctt tttgtcgttc gacttgataa aaaatcactg
    14941 gacgatattg cttccctcta tcttgaaatt gtccaaatgt atctgtggga tcagtaattt
    15001 gccaatagat tgtcagcaac tcgctataac ttatttctct tgtgtcataa atgatctcca
    15061 cagcttccac atgacctgtg taaccaccac ttacttgatc ataatttggt ttttcaacat
    15121 gtccgcccgt gtatcctgac aaaacagata ggatcccttt tttcgattca aacggctcga
    15181 ccatacacca gaaacatccc cctgcaaata ctgcacgttc ttgatagtca gcatcttgca
    15241 tggcatgctt ggaaaagtca tatttttgct ccttatcaaa tccttccgta atcaataaat
    15301 aaaagtccat tacatcaggc gtcagattca ttcggatagc taatggacgt agtgttgctt
    15361 ctagttttga taactgttct ttaacggata tattttctct tatgtcattc tttgtctgaa
    15421 tcaatacttt tcgttcccaa tctcgtgtat tcggattcaa aatcagatta taaagtgttt
    15481 gaatgaatat ttgttcctca tccatagatt ctcctccttt ccgaagaaga caagctagtg
    15541 actttagctg taatttatca taaagtgagg aaataagcga ttacttcatc cccctacaga
    15601 tttttcgtat aaaaaagaga gaatccttat cgacagattc tcctttttaa tcaatttatt
    15661 ttataaatga aaagttaatt taccttttat ggaaaactat tttaaaaaat aaacataaat
    15721 atgcagttaa tgtaacaaga taagaaataa tagtcgttac tgtataactt gtgcttactt
    15781 taatatcagc aactcctttg actccgctta tttgtaccaa tccattagca ttcacctgct
    15841 cctttacagg ttgatgattt atttctacat tcgttcctaa ataagcatat acaggaagtg
    15901 tgattacttg gctcttctca ttagttactc tatatatgat agaactacca gttgttttat
    15961 aaggaaggtt tacccattga ctgccaatct ttgcttcgtg ttttattata tgctccttat
    16021 tctccaatga agcgatagga gaataatcat ataaaaaata atccattcct ttagtggtat
    16081 acgcctctac tgtttctttg tttacaacaa tatgtcccgg ctgagaaatt attttttgat
    16141 tgaaattttt tttactagaa acagttaaag tgattaataa caaaacagtc ataaataaat
    16201 aaaatagttc tcttgtctta ttgtatcttc tatttttgaa aaatatcatc gttaatatca
    16261 gagaagagaa tattatttgc aagcttaaga tccgccaagg aaattgtaac aatttggctg
    16321 gcgtattttg cagaagatac catggcacca aactagttgt agctagccaa gtcaaacacg
    16381 taccataata aatctttttt tctatgtttg ttaatttttt aaataaaatc aggggaatac
    16441 ataaagataa cagaccaact agtcccattg agtaagtagt taagtcgttg ttcatagctt
    16501 cagtcagact atcgaacaca ttagatgcat gagcatataa atttgtcacg ctaggtttgt
    16561 tgattttttg gtagagcatt tgttcaaaca tcggatacca aaaagcactc gtcagaacca
    16621 atgtcattcc tgccgcacta aataattttt taaatatagc aataaaatct gcattcttga
    16681 aacctttaga aaccaaacga ataaaaataa aaatgataat taatgctgta ctcattaatg
    16741 ttgatagtac atggctgtac actagcaaac tcatactcaa tgctagcttt acagacggat
    16801 acttttcttc ttttccagag atgatttgat aagcataaaa taatacaggg ataacaagtg
    16861 ttattgcgat agcttcggca attgcggaac gatagtagat gtctactgtc cgataagcag
    16921 agaaaatata gagacaacta aataaaaatg ccgctttttt ttgttttaaa aaccgctcgc
    16981 cataatgata agctacgagt gttgtacaca agttcaataa ccaaacgtaa ataatatagc
    17041 ttacaatcaa attttttgtc atccaatata acattacagc tggaaaaaaa gtcagaaagg
    17101 gataaaaata atttacacca tatccagtat aattgaatgt ctcataatta atcggactgg
    17161 taaaaatagt cgataacccc ttgattcgag ataagtgaaa agaaagatct ccagtggtat
    17221 aaatactctg tgtatttttg aaaaagatga aagaatatat actactaact ataattaatc
    17281 cactcaatat tactactttt ttatttattt tcattcttct tgtttatctc ctttttagtc
    17341 acatatcgag aatctttcaa cttattaggt gcaaatcgtt taaaacttga atacaccact
    17401 aaacaataag tcagaatact gatccataaa gccatgagag cagttggagt atattttgtt
    17461 gtaatttcta tcttattttc tccagcattt acatccacat tcactaagcc atgtttttta
    17521 taagagtcat ttacgacttt accgtttact tttacctgtg tacctaaata agcatatact
    17581 ggtactgtca ctttttgctt tttgttgctt gccactgtgt aagtaatgtt attatctgca
    17641 gctttatact tccctttttc ccacgattct cctactttta cttcatgttt ttctaagtga
    17701 ggcctttctt ttatcgcttc tgttggggcg taatcgtata atcctcccat atctgatgtt
    17761 gtataacgtg ggagtgtttc ttcattgata acaatatgac cgtgttttga ttggataact
    17821 tttgcgtaat tttcttttgc tgctatagac gtgacgagta gaaacaccac agtagcgcca
    17881 atgattaacc atgaatattt tttattcata gtctttttca taaaaacaag cgttatgatc
    17941 attgaagaaa aaaagatttg gatacctaag atacgccaag gaaactggag taagcttatc
    18001 ggtgtttttt gaaacaaaaa ccatggaaat aaattagtta cgcataccca tgtagaaact
    18061 acgccaacat agatgatttt ttcttttttc tccatttctt tcagtatgaa taaaggaaaa
    18121 atcaacgcaa ccaatcctaa taaccccata gtgtatgttg taatatcgtt atttaacgct
    18181 tgaatgatac tatctgaaat agaaagtgct tctagctgca aatctgtttt gaagggactg
    18241 tgtatttctt gataattcat ctgctgcaac ataggatacc aaaaatagct agttaatgca
    18301 caagtcaaaa aaacagcctt tactaaattc acgaccacaa attttatatc gttttttatt
    18361 tgcttgctta aaaacaaacg aactataatc atacaagcaa tcaatgcact acacaatagt
    18421 gtagttaaga catgagtata cacaagtaga cttatactga cagataaagc gatgactttt
    18481 ttttctctat tgaatatcaa tccataagca taatataaaa cagctgggat aatcgtaaga
    18541 gcgattgctt ctgcaattgc ggatcgataa tagatatcaa ctgttcgata agttgcaaaa
    18601 gtatataaac aactaaataa aaaagcagca tctatctttt taaaaaactt ttctccataa
    18661 taaaaactaa tcagaatcgt acaaatattt aataaccaaa tatatagaac ataacttaat
    18721 attaagttat ttgaaattcc ataaaaaacg actgcaggaa ttattgtaag aaaaggataa
    18781 aaatagttca gtccatctcc ataattgtta aatgttgtat agttgattgg cccttcaaaa
    18841 atagaagata accctttgat tcgtgaaaga tgaaaagata aatcacctgt tgtataaatt
    18901 tcttgagcat ttctcataaa atttactgaa tataaagtac taagtaagat aagaactact
    18961 aaaagaaatg cttttttact tattttcata gatttgttgt tctccttttt taattctatt
    19021 ttcagtatac tagaattgtc tttttttaat aagttttgcg ttaaattttt catagcacta
    19081 gtatgatata ctagaaaaga atcaattttt attactttta aataggagaa aaaaatgaaa
    19141 aaaaacaaaa accaaataat agatatcatt tttatgttat ttctaagttt tgcttacgtc
    19201 atccctctat tcatgtctaa gggaatctat catagtgtca atcaggatac ttactttcat
    19261 ttatctcgaa tcataggact agataatgta tggagttctc cagtaaactt caataacttc
    19321 gatcatcatg gaacaatgat gaatattttt tatccatggc ttactcttta tccagctttt
    19381 ttattttata aaatgatggg aaatcttgtt ttaagttata atatctacta tttttttatc
    19441 acttttttaa caatggtcgt ttcttatttt tctatgaaac aaataaaaaa caatcgttat
    19501 atttcgctgt tattttcgat tatttataca ttttccgcct atcgtgccat tgacatattc
    19561 catcgtgctt cattgggaga agctgtcgct ttaacatttt tgcctttgat actaatgggg
    19621 tgttacgaaa tttatattag agattaccaa aaatggtact ggttgtctat tggcatgacg
    19681 ctagtagtgt atactcattt actttctgtc gctatggttt ctgtatttat tggaggaacg
    19741 ttgtttcttt ctttttattt ttgggaccaa aaaatcgcta gactcttaag tcttttaaaa
    19801 gctaccgtcc tcaccttttt tttaagtgcg ggattcttaa ttccttttat tcaacaatct
    19861 agagcacaag aattgaaagt cccactggga aaagaattaa gtggtatggc tcctagtgat
    19921 atgctgacac atattttaaa taataactat aacaactata ctattggatt attcttattt
    19981 ctcggattga ttggtgcttt tatttttata aaaaaattaa ctgctgatga tttatttatt
    20041 ttctttcttg gtgtatttgt tcttttttgt tcaaccaatt tatttccttg gcaacttttt
    20101 aaccatacac cagttaagag tctacaattt gtctggcggt taaatggttt ttcttcttta
    20161 tttatagcct atacgatgag tatagtgatc tattatattt tttctactaa aaataattca
    20221 tggaaaataa tggtctttac gtttcttgca ctaatattgc atctctcagg agtaagcaat
    20281 tctatccatg ctaacaaaga ttcattaaca cttatagcac ctgaagacgc tgttgctata
    20341 gctgaaaatt acaatcatac tgattatgca aacaaagagt caatctatca tcctgatatg
    20401 attaataatg atcaatattt actaggtaac caggaaataa atccaacaac tcattttatg
    20461 agtaacaaac ttacaattga attagataat tcgaataata aaaatactgt cttgacgaca
    20521 cctatttatc gttataaagg gcaagtggct tctattaatg gcaaactagt acaaacaaaa
    20581 ttatctaagt ttggcacaac tgaattaact attcctccag gtataaataa agtcgttatt
    20641 acttatcagt acacaaaact agcaattgct tcaagatatt tatctatcgt tactttgatt
    20701 ttatttttac tttatcgatt tactttttct aaacaaaaac aaccgagaag agaagtacaa
    20761 cattctcact agatttttat ttaagaaaga ctttttagct gtacacataa acaggctaaa
    20821 aagtcttttt ttatatttct ttaatttgct tcatacatta tttaacatcg atatttatac
    20881 tgtgaaacat gcgtttcatc cattatcaaa gtatttttat cgataataaa cttcatccaa
    20941 aaacagacct tgtgcaggga cggtttctcc tgcatgttgc cgcactttgc tttcaaatat
    21001 ttcatcgatt gtttctatcg gcatggcacc tgtgccgatt tctagtaatg tccccatgat
    21061 aatccgcacc attttatata agaatccgtc tccgacaaaa gtaaaatgaa gcatttctcc
    21121 ctttcgttcg atagataatt catcaatcgt acgcactgtg gatttttttg attttttcaa
    21181 tgcagaaaaa ccaatgaaat catgtgtacc cactaatttt tcgcaagctg cattcagctt
    21241 gtccatatcc aactcttgag ggaaataaaa gctgtggttt cgctcaaatg ctgaaggaat
    21301 cacgttgttc caaacataat agctgtattt tttgcccacc gcattatatc ttgaatggaa
    21361 gcgctcaggc atttcttcga ttcttttgat gacaatatcc cgcggaaggt aacgattcat
    21421 aaagtcgatc atttctgcca gtccaatttc tgtgtttgtt ttaaaatttg ctacttgtcc
    21481 tcgcgcatgg gtgccagcat ctgttcttcc tgatccaata atttctatct cttcatttgt
    21541 catctgatgc aatacttttt cgatttttcc ttgaatggtt ttatcgtcat ctccaagtct
    21601 ttgccatcct gaatatcttt ttccgtcata ttcgattgtc aacttgatgt ttctcatatt
    21661 ttagtggctc ctctgtcttc catttggtct tctctctacc tttttaagat tgtagcacaa
    21721 aatatagctc cttgcttctc taagacagaa gctatacaag aaaactgtgg aaatatatat
    21781 ttcagaaaaa caaaaaacag ccaccggatg aagtatccgc tgactgttca atttgacggc
    21841 tcaagtttta ctgtttgaga tgcaagatac tcaaccaggc aatgggcaca taattaaaga
    21901 aaaaactgat tctttggcaa aaacctattt tattcagtaa tggccaagaa tttatagaag
    21961 gaattcttgc cagtccataa atcgccgcaa aaagcaaact gatcacagac agtccaaggt
    22021 ataagtggct tacgtccatt tgcccatttt ttcgatagat caagaaaaga agaactggaa
    22081 acaataaaaa accactgtaa cctaaacctg atccgatatt atggacccat gtcgagacat
    22141 tccacgaaga ttcatgggta ttgatactga acaaacctgt aaagatacaa tcacctatac
    22201 catagaacgc gatggctgct gtaactaggg tagctaacac acgagatgta ctaacaaatg
    22261 cagcatacaa tgcaggcaat gacaggatga aaaagatccc tgaaaccaca gaccaaataa
    22321 gaaacgcttt tcttacagga ctggaaacct ctccaaatac actgatcacg ctcttcattt
    22381 gatctaattc aggataatac aatcctaata cgtacggagt caaaaaatca ctcagcacgc
    22441 ctaataataa aaagaaaaaa ccaaatttac gtaaaaaatc catgatacgc ctccaataac
    22501 aattacctat gcttttaatt gttattataa acgactttat cttgatttaa cagaaacctc
    22561 tatttacacg aacatctata taactcatta aaaactcgaa tcattttaaa aatactaaat
    22621 atctaacgtt caacgttcac ttaatccatc taaacaatct ttcaatttga ttttttcccc
    22681 acttctttta cgcgcgccta gctctcctat tgcagtaaac aacagatcac ttgacgaatt
    22741 taaggcagtt tccattgaat cctggataac accgataata aatccaatac cgacgatttg
    22801 catcgcgaca tcattagaga taccaaacaa actacatgct aatggaatca ataacagtga
    22861 accgcccgca acacctgaag ctccacaagc agctaaagca gacaaaatac ataaaaggac
    22921 tttcaaaaca aaaggtgctt caatccctaa agaatgtaca gcagtcaacg tcatcaagga
    22981 aatggtaatc gcggcaccgc ccatattgat cgtagcacct aacgggatcg atatggaata
    23041 agactctttt gtcaatttca atctttcagc caacatcatg tttatcggga tatttgcggc
    23101 agaacttcgg gtaaagaaag cgggaagtgc gctttctttc aaacaaaaga aaatcaatgg
    23161 atagggattt tctttaataa acaaaaacgt gaggaatggg tacacaacta atgcgacaaa
    23221 cagcatcgta ccgatcaaaa caagtaaaag ctgcagatac tctgccaatc cagcgattcc
    23281 agtcgtagca attgattgat aaaccaatcc taaaatccct atcggagcaa atgcaataat
    23341 cacttgtacg acttgtgaca acgctataga tatctgatcc accaactctt ttgtcgattc
    23401 tgaacgtgct cgaaaagcta gtcccaacaa tacagcccag aataagacgc caaggtaatt
    23461 cgcatcaata agagattgaa caggattttg tgtgacattc gtcaacatag tagataaaac
    23521 actttccaaa ctttgcggtg ctttttcaga tactgtttct tgtaaagtaa taggaacagt
    23581 aaaaattgaa cttacaataa ctgctacaag tgcagaaagc aatgttccca ctaaatataa
    23641 gatcaaaata ggcttcacga atgtcttatt cccaatttta tgcttggcaa ttgacgccat
    23701 cgtcaagaaa aaaaccagaa taggtgcgat tgccttcaag caactgacaa acaacgttcc
    23761 caatattgaa ataaatgtcc atgtggggaa aaagatacca aacaatattc cgactaaaat
    23821 accaacaaca atacgttgaa ctaacgacat atccaaaaac ttctctaaca aatgaccacc
    23881 ctttcacaat tttaacaatc attgtatcat tttatgcaaa gaaactaaag gtataatttt
    23941 ctaaaaattc taacaataca aacaaccatt tcttgctaat tttgtacttt ttctgtctac
    24001 acgttgtttt ttgagatatt ctaagttagg atgttattaa tgggcaactt atcaggaaac
    24061 gttttcttat gtttttgtcc aatttttttg tgaggtgttt attcttgaaa aaaataaaga
    24121 aggagagtta tggcttcctg acaatctttg tcgcttgtat aatgatggtt ttacagcgta
    24181 tattgatttc agcagtcact attcctacat atgcaacaaa ccctggtcga ttctacatct
    24241 atacattttt acaaacttcc gttgttattg gtgcaaattt gatcccactt tacatgggtt
    24301 accaatatca aaaaatcgcc cgtttaaaag ttttacatta tttaagtcgt tttgccttta
    24361 tattcctaat agccacacta gtttgcaata tcttttttta cgtacaagct ggcagcttga
    24421 atattcgtga ttattggctt atcctaacac ctatcagtca gaattacttt acttatgctg
    24481 tttcttgtac tttgcttttt tgtagtattc cttatacagt tggattttta aataaattat
    24541 cgaaaacaac aattaaatat ctactattat ttttaacaat tggattagtt ggctgctcta
    24601 ctctatttaa taaagacccg tttgcattac agaatggaca gagtatttta tggatttttt
    24661 atttattttt aatgggttat ggtttgaaag aatggaactg gaaagaaaag gtccgctata
    24721 agcttcctca agtatttatc agcttgattg ttttattcgg tttgattatt ttgatgacac
    24781 aaatttcact catcatcaga ggaaatacag atacagcgct gaggttttct aaaccatatt
    24841 ctctcttcgc aatgtattat actttctctt cgtttttatt tttagaaatg ctatctgata
    24901 aattaggtat aaaaggaaga ggaaattttc ttgctgtatt tttgattgtc actcaagtta
    24961 tatgtaattt ccctttagtt tcttatcgcg tatctacatt tatgaaacgt gaatttccaa
    25021 actcagggaa ggcatggctg ataaatatcg ggcagttttt tatttgatat ttattagcag
    25081 tgtccctcct agttatcttt ttattttata tacaaaaaat ccgtttcatt catcgtttca
    25141 tcgaatactt cgcttttgat tcgattgaac aattaactca gcgttggaaa aaaagaattc
    25201 attggctttt tgttcataaa agttattttt tagttgcagc atttttttac tgctttacct
    25261 tcgtgcagat tttcgtttta gaaccaaaag aaggttggaa agaaactatc caggttgcct
    25321 taaaaatatt tactcagcga caagcaccca tgatcctgac aatcatcatt attatgggat
    25381 ttttcttctt actattgttg atttccaatc gtttttggta cgccttgact gctaccttga
    25441 tcattaactt actgctcact atttcgacag taatcaaaat ggaaatgcgg gaagagcctg
    25501 tctttccttc agatttaaaa atgctgaccg gattgtcaga actattatcg atggttagcc
    25561 cagtcttatt aatcgtgggc ggattgatcc ttctcctatt attgattact agtattattg
    25621 tacaacgtag actacaaaaa cagtattcct tgaagattca ttggaaacgt cgatttatca
    25681 gtatcgctgt attattagga tgtttttcgg gtgtcttttt cattaatcac aaaaattcgc
    25741 cttctttctt gctgtttaat ttatttaaag taaacaagac tttctttaac cagcaaggcg
    25801 ccgtaaaaga taacggacca gtcattcaat tcctaaacaa tatcgacatt aaaatcatgg
    25861 aagaacctgc tggctattcg aaagaaaaaa ttaatggaat aatgaaaaag tacaacacaa
    25921 aggcagatga aatcaaccaa acaagaagtg actggttaaa caatcagacg atcgttctca
    25981 atttaagtga aagcttcagt gatcctgcac gtgtccctaa tctgacagta cctacagacc
    26041 ctattcctta catcaaaagc attataacag aaggaacagg aggattgatg ttatccgtcg
    26101 gttatggtgg cggaacagct aacatggaat gggaaggact gacaggcttg agtatcagta
    26161 acctgtctgc ttcgttagtt acaccttata cccagctagt ggagcgtcag aaaataagtc
    26221 caaatattac cgatctattc gatgaaaaaa tagccatcca tccttttaca gctgctcttt
    26281 atcgcagaaa acaagtgttt gataaatttg gttttgacag gttctattat ttagacagtc
    26341 cggataaatt aacttatacg gataaaatag aacaaagccc acgcatatcg gatgattcag
    26401 catacaaaga aaccttgaaa gctttacaaa gcaatacgga aacgtcacaa ttcatccaat
    26461 tatccactat gcaaaaccat atgccttatg atgattacta tagtgaattg aactacactg
    26521 ctgaaggaac tgctgtcatt cctaatagaa gaaaagaatt acaaacttat atgcaaggcc
    26581 ttcactatac cgatacagca gtaaaaaaat tcatccaaga aatcgacaaa atcgataaac
    26641 cgattacctt tgttttttat ggtgaccatt tacccgcaat ctatagtggg aatgattcga
    26701 aaaaatatgg attagaacag caccagacag attatttcat ttataacaac aagtatagtc
    26761 gtgaacaagc agctacgtta aataaagaag ttgttgctcc taataacttt ggtgctatgg
    26821 ctcttgagca tgcgaatatc aaagtaactc cttactatgc gttgttgaca gaagtatcta
    26881 atcatctacc tgcattaaca attgatccta cagaaagctt gtctaatcgt tttaatggaa
    26941 aacaggtctt tgttacagag gatagtcaaa ttatccaaga aaaaaattta actgagaaac
    27001 aaaaagaaat tctagaagat tatcgtctga tacaatatga tcttgttgca ggtaaccagt
    27061 acagtgcaac atgggcgaca caaaaaataa aagactaaca aataaactga gcaaggggtt
    27121 gtgataaaag acatgtcaca accccttgtt tatctcatta atttgcttgt ccataaacga
    27181 tttgattaaa gccatttttc aattcattat tcgctgtctc gttgaagtaa agataattat
    27241 cgggattccc ctgattttgt tgtgtcacca atacttttgg ctgattttgc tgaaacccga
    27301 atagatagac gtgctttttc agatatggtt ctgtctctgc atcggaatag acggctgtga
    27361 tttgatagtc tgcttgacct gtcccacttt ctgaccattc tgcagaaaca ggcactccac
    27421 caatagccat cttccactcc ccgttcaaaa tagcttgagg gactttaaga ccatataagt
    27481 ccacactcat ttgattcgta taacttttgt attgttgtcc cattgtttgg ccccatttgg
    27541 tcataaatgc ctctaattgt gcggatttgt ctgcattcca cagtgtttgt tcaacttgtt
    27601 cgctgctttc tgaagaggaa gatgaagcct ctacagaaga acttgaagta gatgtttgag
    27661 ttgtttcagt cgttttggaa gactctgacg aagtagaggt atctgctgtt tctgtacttg
    27721 ctgcagttga cttgctctct gtgctagttg ttgtagtagt tgcaactgtt gctttcgctg
    27781 tttcttttgt tgattctttt ttcgattcat tcccgttggt acaaccagct aacaaaaaag
    27841 ctagaccaat tgttactaaa atcctttttt tcaattaaaa aactcctttc gattactccc
    27901 acttctttat tctatcaaat aagcaatgtg attttattga tttttcgtat tttctcatca
    27961 atctttcaaa tcttctaaaa actgagaagc acccgctgat gttgtctgta atacatcagc
    28021 gggtgcttct tttattttaa tcttcgtgat tttccaattt acctcacgct ttatatttgt
    28081 atttgaagaa acatagactt ttagtcatca tcgtcttcct catccaaatc ttgtcggaca
    28141 gtttttcctt ggagtctttg tacttggtag atgatttctt tattgaaact taaaagttca
    28201 acaagcaatt gttgcaagcc atatttttct tctttttcag ctaatttgat tgcccgtgtg
    28261 ataaagacat tttggaaatc aaaatctttt gcaaagtttt ccaacattgt tgaggctggt
    28321 aaatatttgt tttgaccatg ttcgtaaatt tttccataac gcatgacttc ttctaaagta
    28381 gacggaacga tttcattttc aatcactaac tcatgagaga ttttattgaa ccatctcgct
    28441 tcttgatcag catattctga aaaaagggta cttaattctt gcgaagatgt acccaaaaca
    28501 tacaattgtg cttggatcaa tttcatttct tctaatttaa aatttgctaa tacatgcccc
    28561 accatggcgc cagcagttgg tttatgatgg tcgatatctg cttgttttgt ttcttgaaca
    28621 aatttttctt ccactatatt cattgattta cgcctctttt acttttgctg attggacttc
    28681 ataacctaat ttttccacaa ctgcagctaa ttgttcaggg tggacttcat ctggatcaaa
    28741 gtttgctttg attttgctcg cattaaacaa aactttcgta ttgcttaccc caggctgttg
    28801 agaaaccgca tgttcgattt tttgcataca gcttggacaa gctagtgtat ctaattgcat
    28861 aataattttt ttcatcgtag ttcttccttc ctttttcttg atagtcttat tctagattac
    28921 agcttccata aaaaaattga ttcttatcaa gttttacgat ttttcttctt tgctttcgtg
    28981 tccaaaatac aataaacgca tcgcgttaaa gataacgatc aagatactag cttcgtgaac
    29041 gaacatccca ctagccatgt aaataaaccc ggcaaataag ccgttgagta aaaataagac
    29101 gacaccaatg gcaatcacta cattttcacg ggtatttaaa acagttttct tagccaagcg
    29161 gtaggcatga accaacgatt caaaattaga ttgcatcaag acaacgtcac tcgtttcgat
    29221 agcaacatcc gttcctgatc ccatagcaat cccgatatca gccgcagcca aagagggaga
    29281 atcattgatt ccatctccaa taaaagcaac gcgcaatcct tgttcttggt attttttcac
    29341 aaattgaacc ttttcgtctg gtaataattc agcatggact tcatcaatgc ctaacatatc
    29401 agctacataa tcagcagtca tctgattatc acctgtcagc atcactaact gttttgctcc
    29461 tgcttgtttc aacttagcta attgttcagc gacttctggt cgaatcacat ctgacacacc
    29521 tatgatttga gtgatttttt tgtcgattgc tacaataatg accgagctgc ctgtttgttg
    29581 taattgatgt aaatcttgta cttgctttgg tgtgagagaa agtttgtgag cggcaatcaa
    29641 tttggtgttg cccgcacaaa ctgtatgacc atcaatctca gctgtgattc cttgtccctt
    29701 gattgtctga ttatctttga ctgctaattg ttgaaaatca atgtctttct cctgagcata
    29761 atggatcacg gcgcgcccca gaggatgatc ggataacgtt tctaatcgag ccactaaagc
    29821 taagctgtcc ccttcatcct ctgtatagtt cttgatagtg gatacagcag tagttccttt
    29881 ggtcaatgta ccagttttat caaagacaaa agtatcgatt ttggaaaaag tatccattgc
    29941 ttcaccgcct ttgactaaga tgccccgttt cgctccatta ccgattcctg ctacattcga
    30001 aacaggcgca ccaatgacta aagcgccagg acaaccaaga accaaaacag tgattgctaa
    30061 acgaaaatca cgactaatca gaccaacaac caacgcaata agtaaaacgg ttggagtata
    30121 atactgagaa aatcgatcaa tgaatttttc cgcatgggat ttggtatctt gtgcttcttc
    30181 tactaattcg ataattttgg caaaggtcgt atcatctccg accttagttg cttcaatcgt
    30241 cacgtagcca tctgctaaaa tcgttccaga gaagacttca tctccactga ttttgacgac
    30301 ttgtcgtgat tcaccagtca ctgcggcttc atccgcatac cctttcccgt caatgactcg
    30361 tccgtccaca ggaatagctc caccagtttt tactaagacg atatcgcctt cttccacgtc
    30421 atcgatttcc acttcttcag tcgttccatc ttcttggaca acactagcag tagatggggc
    30481 catctcggtc aaagccttga ttgactcccg cgttttggtc aatgtttttt gttccagata
    30541 gctaccaaac aaaaacaaga atgtaacgat agctgactcc ttgtattctc cgatcataaa
    30601 ggcaccaagc acagcaatag taacgagtaa atcaattgag ataactttga ctttcactgc
    30661 ctgatacgca tggatcataa taggtatgac cgcaataata gaagctatgg ctagagcaat
    30721 gttatagaac aagacaaaac cagtaaattt tccgataaaa ccaaaaataa tcaacacagc
    30781 tgataaggcg gtgatatgat ttcgatgctc ttggatccac ttttgtaatt tcatctttct
    30841 ccctcttttc ctttcattct tacgagacta agtatagata cttatccatc ataaaaaatt
    30901 gaccactatc aagtttagtc aaaaaggcgg ttttcttttg gtagaataca ctacaaaaaa
    30961 tagttagcaa gaagactcac taaaagtgat cttgccaact attgtcgttt ctatcgaaat
    31021 tttcattgaa tgattttcct gtacagcata catcggagat ctatttcttt atctaagatt
    31081 tttatcttgc taattttttc ctttagtctt tcaaatccac gtcgttcata gaattgatac
    31141 gtacagccac tatctgtaaa aaggaatatt tcttttcctt tctctctttt ttcaaattct
    31201 gttagcaact tggtaccgat gccttttgtt ttcaaatcag gatttgcagc taaaaaaatg
    31261 atctctccat ctggcgaatg cttctttaaa tatgctgcaa gcatttgttc atttgctgtc
    31321 tcgtaagctc ctttactttc tttagcaaat atattttgta aaaaatcgaa tatcttaaca
    31381 tatatttgct gtccaaaaga acgatgtttc tttttttctc cttttatctc cgccagcaaa
    31441 acacctgcca actcgtcctc tgcatacaaa gcaagaattt gagttgctcg attcaactcc
    31501 atatacaaaa aatatcttcc gtaaagattc agcagaagtt cattttccat atatctctca
    31561 aagttcattc cttcaacagc aaaatagata gcttttttgt aatctttctt ttctaagggt
    31621 ttaaaagtta gtttcatcat ttcgcctcct caacttcaat gattgtgtta tacttacatt
    31681 ataatagaca cagtgtctac tatctataat caatcatcta taagtttgtc gattaagcga
    31741 cagctttgct ttgatatgtc ggataaggag ataggtaaat gaaaaagaca gatagacgcg
    31801 tcaaaaaaac agaaaaagct ttggctgaaa cactatccac attattagta aataaaaaga
    31861 ttcaagcaat cactatacgc gaactaacag aaacggcaga tgtacatcgt tcgacttttt
    31921 atacacatta taaggatatc tatgatctat atgat
//
LOCUS       DAZKOW010000019        31459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000019 DAZKOW010000000
VERSION     DAZKOW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.019"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..604
                     /locus_tag="ACG912_000918"
     CDS             212..604
                     /locus_tag="ACG912_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0512255.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACG912_000919"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACG912_000919"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFQ0512256.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACG912_000920"
     CDS             2698..2943
                     /locus_tag="ACG912_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512257.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3165..4775
                     /locus_tag="ACG912_000921"
     CDS             3165..4775
                     /locus_tag="ACG912_000921"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFQ0512258.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4752..4964)
                     /locus_tag="ACG912_000922"
     CDS             complement(4752..4964)
                     /locus_tag="ACG912_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512259.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5067..5936
                     /locus_tag="ACG912_000923"
     CDS             5067..5936
                     /locus_tag="ACG912_000923"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFQ0512260.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="ACG912_000924"
     CDS             6101..7372
                     /locus_tag="ACG912_000924"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0512261.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="ACG912_000925"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="ACG912_000925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFQ0512262.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="ACG912_000926"
     CDS             8763..9026
                     /locus_tag="ACG912_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFQ0512263.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="ACG912_000927"
     CDS             9471..9920
                     /locus_tag="ACG912_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0512264.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="ACG912_000928"
     CDS             9977..11395
                     /locus_tag="ACG912_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0512265.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="ACG912_000929"
     CDS             11395..12255
                     /locus_tag="ACG912_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFQ0512266.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="ACG912_000930"
     CDS             12429..13202
                     /locus_tag="ACG912_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0512267.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="ACG912_000931"
     CDS             13349..13714
                     /locus_tag="ACG912_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFQ0512268.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="ACG912_000932"
     CDS             complement(13862..15070)
                     /locus_tag="ACG912_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0512269.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="ACG912_000933"
     CDS             complement(15089..15367)
                     /locus_tag="ACG912_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512270.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15560..16216
                     /locus_tag="ACG912_000934"
     CDS             15560..16216
                     /locus_tag="ACG912_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0512271.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16658..17455)
                     /locus_tag="ACG912_000935"
     CDS             complement(16658..17455)
                     /locus_tag="ACG912_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFQ0512272.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="ACG912_000936"
     CDS             complement(17452..18636)
                     /locus_tag="ACG912_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFQ0512273.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="ACG912_000937"
     CDS             complement(18661..19908)
                     /locus_tag="ACG912_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0512274.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG912_000938"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="ACG912_000938"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFQ0512275.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="ACG912_000939"
     CDS             21888..22202
                     /locus_tag="ACG912_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFQ0512276.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="ACG912_000940"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="ACG912_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFQ0512277.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /gene="tmk"
                     /locus_tag="ACG912_000941"
     CDS             22887..23531
                     /gene="tmk"
                     /locus_tag="ACG912_000941"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFQ0512278.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="ACG912_000942"
     CDS             23546..23875
                     /locus_tag="ACG912_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFQ0512279.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="ACG912_000943"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="ACG912_000943"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFQ0512280.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="ACG912_000944"
     CDS             24863..25687
                     /locus_tag="ACG912_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFQ0512281.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="ACG912_000945"
     CDS             25680..26027
                     /locus_tag="ACG912_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFQ0512282.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG912_000946"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="ACG912_000946"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFQ0512283.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="ACG912_000947"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="ACG912_000947"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFQ0512284.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG912_000948"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="ACG912_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFQ0512285.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG912_000949"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="ACG912_000949"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFQ0512286.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
BASE COUNT        10114 a         5635 c         6634 g         9076 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtag ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcgaaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataa
//
LOCUS       DAZKOW010000020        31109 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000020 DAZKOW010000000
VERSION     DAZKOW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.020"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            94..576
                     /locus_tag="ACG912_000950"
     CDS             94..576
                     /locus_tag="ACG912_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512287.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            773..1663
                     /locus_tag="ACG912_000951"
     CDS             773..1663
                     /locus_tag="ACG912_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFQ0512288.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1988..4459
                     /locus_tag="ACG912_000952"
     CDS             1988..4459
                     /locus_tag="ACG912_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0512289.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(4562..6298)
                     /locus_tag="ACG912_000953"
     CDS             complement(4562..6298)
                     /locus_tag="ACG912_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0512290.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6295..6999)
                     /locus_tag="ACG912_000954"
     CDS             complement(6295..6999)
                     /locus_tag="ACG912_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0512291.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7130..7633
                     /locus_tag="ACG912_000955"
     CDS             7130..7633
                     /locus_tag="ACG912_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFQ0512292.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7593..7931
                     /locus_tag="ACG912_000956"
     CDS             7593..7931
                     /locus_tag="ACG912_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFQ0512293.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7952..9370
                     /gene="ffh"
                     /locus_tag="ACG912_000957"
     CDS             7952..9370
                     /gene="ffh"
                     /locus_tag="ACG912_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFQ0512294.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9482..10060
                     /locus_tag="ACG912_000958"
     CDS             9482..10060
                     /locus_tag="ACG912_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFQ0512295.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10064..10585
                     /locus_tag="ACG912_000959"
     CDS             10064..10585
                     /locus_tag="ACG912_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFQ0512296.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10664..11218)
                     /locus_tag="ACG912_000960"
     CDS             complement(10664..11218)
                     /locus_tag="ACG912_000960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0512297.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11471..11746
                     /gene="rpsP"
                     /locus_tag="ACG912_000961"
     CDS             11471..11746
                     /gene="rpsP"
                     /locus_tag="ACG912_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFQ0512298.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11758..12009
                     /locus_tag="ACG912_000962"
     CDS             11758..12009
                     /locus_tag="ACG912_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFQ0512299.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12134..12925
                     /locus_tag="ACG912_000963"
     CDS             12134..12925
                     /locus_tag="ACG912_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512300.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13095..13616
                     /gene="rimM"
                     /locus_tag="ACG912_000964"
     CDS             13095..13616
                     /gene="rimM"
                     /locus_tag="ACG912_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFQ0512301.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13606..14367
                     /gene="trmD"
                     /locus_tag="ACG912_000965"
     CDS             13606..14367
                     /gene="trmD"
                     /locus_tag="ACG912_000965"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFQ0512302.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14503..14850
                     /gene="rplS"
                     /locus_tag="ACG912_000966"
     CDS             14503..14850
                     /gene="rplS"
                     /locus_tag="ACG912_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFQ0512303.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(14982..16118)
                     /locus_tag="ACG912_000967"
     CDS             complement(14982..16118)
                     /locus_tag="ACG912_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0512304.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(16234..16800)
                     /locus_tag="ACG912_000968"
     CDS             complement(16234..16800)
                     /locus_tag="ACG912_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HFQ0512305.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(16936..17589)
                     /locus_tag="ACG912_000969"
     CDS             complement(16936..17589)
                     /locus_tag="ACG912_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512306.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(17639..18067)
                     /locus_tag="ACG912_000970"
     CDS             complement(17639..18067)
                     /locus_tag="ACG912_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0512307.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(18072..18446)
                     /locus_tag="ACG912_000971"
     CDS             complement(18072..18446)
                     /locus_tag="ACG912_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0512308.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            18750..18935
                     /locus_tag="ACG912_000972"
     CDS             18750..18935
                     /locus_tag="ACG912_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512309.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            18948..19196
                     /locus_tag="ACG912_000973"
     CDS             18948..19196
                     /locus_tag="ACG912_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512310.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            19227..19397
                     /locus_tag="ACG912_000974"
     CDS             19227..19397
                     /locus_tag="ACG912_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512311.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(19499..19966)
                     /locus_tag="ACG912_000975"
     CDS             complement(19499..19966)
                     /locus_tag="ACG912_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512312.1"
                     /translation="MNYLIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFL
                     VKHDRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLL
                     SPIPLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            20125..20442
                     /locus_tag="ACG912_000976"
     CDS             20125..20442
                     /locus_tag="ACG912_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512313.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            20375..20854
                     /locus_tag="ACG912_000977"
     CDS             20375..20854
                     /locus_tag="ACG912_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HFQ0512314.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            20871..21047
                     /locus_tag="ACG912_000978"
     CDS             20871..21047
                     /locus_tag="ACG912_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512315.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            21304..21735
                     /locus_tag="ACG912_000979"
     CDS             21304..21735
                     /locus_tag="ACG912_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512316.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            21741..22058
                     /locus_tag="ACG912_000980"
     CDS             21741..22058
                     /locus_tag="ACG912_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0512317.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            22177..22863
                     /locus_tag="ACG912_000981"
     CDS             22177..22863
                     /locus_tag="ACG912_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0512318.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            22838..24187
                     /locus_tag="ACG912_000982"
     CDS             22838..24187
                     /locus_tag="ACG912_000982"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0512319.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            24190..24651
                     /locus_tag="ACG912_000983"
     CDS             24190..24651
                     /locus_tag="ACG912_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0512320.1"
                     /translation="MGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIARNKNN
                     EVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNWDKRR
                     NKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            24653..25153
                     /locus_tag="ACG912_000984"
     CDS             24653..25153
                     /locus_tag="ACG912_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010050472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFQ0512321.1"
                     /translation="MTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATKSGAEFIDID
                     LIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSYNGFDELLAD
                     FVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGPERSRATTVR
                     NDNLPF"
     gene            25168..27480
                     /locus_tag="ACG912_000985"
     CDS             25168..27480
                     /locus_tag="ACG912_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFQ0512322.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            27727..28044
                     /locus_tag="ACG912_000986"
     CDS             27727..28044
                     /locus_tag="ACG912_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFQ0512323.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            28041..28202
                     /locus_tag="ACG912_000987"
     CDS             28041..28202
                     /locus_tag="ACG912_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0512324.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            28205..28504
                     /locus_tag="ACG912_000988"
     CDS             28205..28504
                     /locus_tag="ACG912_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512325.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            28504..28818
                     /locus_tag="ACG912_000989"
     CDS             28504..28818
                     /locus_tag="ACG912_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFQ0512326.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            28811..29341
                     /locus_tag="ACG912_000990"
     CDS             28811..29341
                     /locus_tag="ACG912_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFQ0512327.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            29338..29607
                     /locus_tag="ACG912_000991"
     CDS             29338..29607
                     /locus_tag="ACG912_000991"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512328.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            29537..29740
                     /locus_tag="ACG912_000992"
     CDS             29537..29740
                     /locus_tag="ACG912_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFQ0512329.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            29737..30024
                     /locus_tag="ACG912_000993"
     CDS             29737..30024
                     /locus_tag="ACG912_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512330.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            complement(30057..30308)
                     /locus_tag="ACG912_000994"
     CDS             complement(30057..30308)
                     /locus_tag="ACG912_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512331.1"
                     /translation="MTMWSMLLFWIPVCIGIVAFCYFVKHSRTNKLLMLSFLPIVFFI
                     VQIVKYTYIESQEIFIFYVVGLFISVVFFIMILSYFYKK"
     gene            30511..30924
                     /locus_tag="ACG912_000995"
     CDS             30511..30924
                     /locus_tag="ACG912_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0512332.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
BASE COUNT        10446 a         5385 c         6424 g         8854 t
ORIGIN      
        1 cattatctct aaaaaaaaag tttttccccc taattttttt agatagaaaa ttgtatagta
       61 tgatgagaga aaaagtagtt tgggggaacg ttaatgagaa gaaaagcagt aatcgatcgg
      121 ctttatttat ttttttggtt ttttcctatc gttttgctac cttttacatg ggtaaatgct
      181 gttgtacaag gtgcggagat attaggcctg atccaaggaa gcggatttgt ggtttctagg
      241 aattttccag ctttgactgt tttctattta gtgataagtg taggtctctt attaccaaaa
      301 tctctgatca atcactacaa ttggcagctg ctttactttt cttgcttatt agtatttaca
      361 tctatgtttt ctttgctgcc tcgcttgatt atcggcccta atggtttgag cgagagtgga
      421 ttgaaggatt cagcttattc ctccatgtat ttcttgacga tcaaaccatg gttctattta
      481 gtaattgcct catttattct tgcaacattt ttttacttct ggacagaaat caggataaaa
      541 agtgaacgat ctgtatctat aaaaaataac tattgatggt ggatcatcag taaaaaagca
      601 tatgagctgc gtaaaatgca tgtttctcat atgctttttt tattattatg taaagaatgc
      661 gaaaaacgtt tacatgattt ttacataact taactatatt tttacagcaa ccatacgagg
      721 gatttacagt cgactgataa actttagttg taaataagaa aggagcaata aaatgaaaaa
      781 agtattgttt actctaggta tgatgggaat tcttttagcc ggatgcggct caggatcagg
      841 aaatacagat ggctctgcca cacaaaataa ttcaagtgac tcaaatgaac aagtaaaaat
      901 cgttgcggta ggttcgacag cattacagcc gttagtcgat gcagcacaag aaagctttgt
      961 acaagaaaat ccaaattatc aaatctctgt acaaggagga ggaagcggaa ctggtttgag
     1021 ccaagtcgaa gctggagcag ttactatcgg aaactctgat gtatttgcag aagaaagaga
     1081 cggagttgat gcttcaaaat tagtggatca taaagtagca gtggtcggga tggcgccaat
     1141 cgtcaataaa gatacagatg tcaaagatat caccaaacag gaattgattg atatctttac
     1201 aggaaaaatc actaactgga aggaagttgg cgggaaagat caaaaaatca acgtcgtgaa
     1261 ccgtgcaaac ggcagcggta cccgcgcgac attcgaaaaa tggggactgg atggtgctac
     1321 ccctgtgcag tcccaagaac aagattcatc aggaacagtt cggcagctgg taagtcaaac
     1381 accaggagca atcagctatt tagctttctc atatctcgat gattccacac aagcactaag
     1441 tatcgacggt gtagaaccaa aagaagagaa tgtagcagat aacagctggg gaatctggtc
     1501 atatgaacat atgtacacga acggaaaacc ttcccctgaa gtccaaaaat tcttagacta
     1561 catgatgaca gaagaaatcc aagaaggacc tgtcaaagaa ttgggctatt taccaatcac
     1621 aatgatggaa gtagaacgtg atcacgaagg aaatatcaaa taactagcag ataaacatgc
     1681 cacggtagtc gttactatta aggaaaaatc aactatgttt cccacatata catttttcct
     1741 ttaattgtga aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
     1801 ctgattaaac accgtttatt cgaaaaaaag agctacgaca tagaactgtc gtagctcttt
     1861 ttttgctata tgacaggaat gaaaggtgtt tgcaagctta atgtgaggaa cactttccat
     1921 atctgttcta tactggatag gagaagtttg gaaatatcca gaaaacgaaa tcaatcgagg
     1981 tagccacatg aagaataaat ggataaatcg aagtaaaaaa aagaaagtaa cacaaagatc
     2041 atcagataaa gtcatccgtc tttttccatc gtataaagaa ggattaacgg cagatcaagt
     2101 caaggaacga atagaaaaag gtgcagcaaa taattcagtc gatcccacat tcaaaacaaa
     2161 tcagcaaatc gtattggaaa acatatttac gtatttcaat ttgatctttc ttatcttagc
     2221 cattttgctt tgtttagtag aatcctataa gaacttgact tttttaccag ttatcatagc
     2281 caatacagga atcgccattt ttcaggaaat tcgatccaaa aagatcttgg acgaactaaa
     2341 tgttttaaat acagcaaaag tcaatgtcat acgggacggg atagaacaaa caatagatat
     2401 agaagacctt gtaatagatg acattgttat tttagagact ggacatcaaa ttccagcaga
     2461 tgcagaggtc gtggaaggaa aactgcaagt taatgaggct ttattgacag gcgaagcaga
     2521 tgaaataacc aaagaaatag gcgatccatt aatgtccggc agtttcattg tttccggaaa
     2581 agcttacgcc cgattagaca aagttggagc agattcgtat atttcccagc tgacagtcaa
     2641 agccaagaca atgggagaag gagaacagtc ggagatgatt gcttctctta atcaattgat
     2701 caaatggatt gggatcatca ttattcctat cggtatcctg cttttttctc aaagttactt
     2761 ttttaatgga aacacgatca aagaaagtgt cgttgcaatg gaagcagcat tgatcggtat
     2821 gatacctgaa ggactgtact tgctgacgac gattgcatta gcattaagtg cgactaaact
     2881 agcaaagcaa agagttcttt tacacaatat gaaaagcatt gagacattgg caagggtaaa
     2941 tgtcctttgt gtagataaaa caggaacgat cacagaaaat aaaatgtctg ttcaaaaagt
     3001 gatcgtctct aaaaaacaag aaataatcag tcagtcagat caattggaag catgcattgc
     3061 tgactatgca aaagcgatgg caggagacaa tgcaacaatg gaagctgtta aagcatattt
     3121 tacaaaaacc actgatcaat cctatcacca tgttattcct ttttcttctg tccaaaaatt
     3181 cagcagtgta gcgttggcag acaaggtgta tgttttagga gcgcccgaaa tggttttgcg
     3241 cgatcaattg ccggaatatg cggaagagtt tgtttctttc gctgaaaaag gctatcgcgt
     3301 gttggtcttt ggtgtgtacg atgggattct tgaagaacca gtattgacag aagcagtcac
     3361 accattaggc tatattctga ttgctaatcc tatcagaaaa gaagcaagag ccacatttga
     3421 ttacttcaaa aaacagcaag tagccatcaa agtgatttct ggagataatc cgctcaccgt
     3481 atccaatgta gctgtccaag cagggatttc acatgctgat cagtacgttg atgcatctaa
     3541 attatcagaa gaagaatatg aagcagctgt cgaaaaatat acagtttttg gacgcgtcaa
     3601 gccagaacaa aagaaaatct tcgttcaatt gataaagcgt aacaatacag tagctatgac
     3661 aggtgatggc gtgaatgata ttctagccat gaaagaagca gactgcagta tagcgatggc
     3721 ttccggaaat gaagccgcca tgcaggcctc acaagtggtc ttacttgatt ctgacttttc
     3781 acgcatgcca gaagttgttg cagaaggacg gcgcgttgtg aacaatattg agcgatcagc
     3841 cagcttgttt ttagtgaaga atattttctc ctttttgctc tcactgtttt cagtgatctt
     3901 tgctctgacc tatcctttag aaccatcaca aatcacattg attagtttgt ttactatcgg
     3961 acttccatct ttcttgcttg ctctagaaga aaacaaaaag cgaatcagag ggaaattcat
     4021 catgaatgtg atggaaaaag ctgtgccagg tggactaaca gatatgatcg tagtcggtgc
     4081 gttggtgatc tgcggggtga ctttgaatct aaataaaaca gatgtttcta cagcatctac
     4141 catgcttttg attgcagtag ggttcctcgt tctctataaa atctgttcac cgctgaataa
     4201 attcagaagt cagatcattc ttttctgtgc tagcggtaac tttttttcgg tcatcttttt
     4261 gcataagctg ttttcaatca ccggtatctc agctgtatca cttttgctgt tgagcattct
     4321 atttttctcg gcagattcga tttttaggca tttgaccaca ttggtagaat atttgtttga
     4381 acgtaaaaat gaaaaagagc aaaagccgtt tagtcgcgta gctaaaaaat ttctatcgat
     4441 atttaaacga gaaaattgac gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc
     4501 gtgtatcttt cgaagcagga tgagtcatcc aactttaaaa cgcacgagca gttgctcttt
     4561 tttacatttt cggaatggta acggtgaaag tcgaacctgt tccaagatga ctgtctacag
     4621 taatcgtacc attaaggagt tgcacgtagt cttttacaat cgctaaccct aatccggtcc
     4681 cgcctgaatg tcggcttcgc gccttgtcta cgcggtaaaa acgctcaaag atccgttcct
     4741 gatcctcaat gtcgatacct ataccaaagt cttggacaga aaacgataga tcatctgttg
     4801 cctgatagcg gataataatc tcgctgtcgc tttttgaata ttggacagca ttttcaatca
     4861 gatttttgat aattggataa aggatatctg ttttagtagt aaatggttcg ttctttccaa
     4921 tcaatcgaat cgtcaaccgt ttcttttcga tgattggctg ataattttga acgatttgct
     4981 ggaaataagg ttctgtataa attgtctgga tctcgtaaga attctcgcca tcttttgaca
     5041 gctggatgat ttcccgaatc agtttatcta accggatcgc atctttttgc atgatctcaa
     5101 gaaaagaagt cagagtttgc gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac
     5161 caatcagtga agtcacagga gtttttaatt cgtgagagac gttgccaacg aaatcctttt
     5221 gtagcttttc taaacgtcgc actttcgtta agtcatacgc cacccctaga atctgtccgg
     5281 taccttctga attagtaaag tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa
     5341 gggtgatctc ctgatggatc agcggatgct caggagtcac ttgatgaatc agctgaatga
     5401 gttttggctc ttgaatgact tctgtgtacc gctgttcagg ttgatagctt accacgttta
     5461 aatgtagctg catctttgga ttaagcagta gtaaacgaga atcagcatca tcaatcagga
     5521 atacgccaat catcagttcg ttcagtaacg tatataattg ctcttctgtg gtagtacacg
     5581 cccgatacat cttactcatt tgttcgctca acgcattaat cgtctgatac aattcttccc
     5641 attgatcggg tgtttgcatg attacttccg cctgattagg attttgcacc atttttttta
     5701 gtacgggcag cacggttttc aatggctggt ttttttgata gatcagataa tagatcattc
     5761 ctgcaagaac aaggaagaaa gacagaaaga agaaaaagac ccaacggcga aaactctcag
     5821 ttctcggtaa aaaaccgctt gtcggttctg cgatacgtaa aatggcttct aactggccac
     5881 tttttttgac tggtaatgcg acatataaaa gatcctcatt caaagtagta cttttgcgca
     5941 aagcgctacc taggttccca ccattcaata cggctttgat ttcgggacgg gagtttcgct
     6001 gttcattcaa tgtttcattg tttgtgtcaa ataagatctt tccagtagca tccattaaag
     6061 taatccgttc gttcgactga tggacgaatt cttccaaagc agttttgttt tgcgctgctt
     6121 ggaggttttc gacatctaat tgccggatca gcagttgtgc tttttttgtc aggtagcttt
     6181 cttgctgttc cacgatctgc tgttgaaaat aatgggaaat caattgccag ccaccaaaaa
     6241 acaaactaac taaaagaata agaaagaaga gacgttcata aatttttttc catttcatcg
     6301 ttttggttcc tgaaacttat agccaaatcc tctaatagtc accaagtact ttggatgttt
     6361 tggatctcgc tcgatttttt ctcgcaaatg actaacgtgt acatctacga ttcggctttg
     6421 tcctgcgaag tcaaaattcc aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc
     6481 ttttcgtttc atgaaataaa caagtaattc aaattctttc ggtgttagtt caatcagatg
     6541 gtcgtctacg gtcacttgat aattggtcaa atcagcagtg atttgtccaa tcgatagata
     6601 ttcaggctca gcttcatctt ttgattgctt acgaggttca atccgtctga atattgcttt
     6661 catacgggct aatacttcgc gaggactaaa tggtttggtc aaatagtcgt cagcacctat
     6721 ttctaggcca atgatccgat ccacttggtc atctttagcc gtcagcatca aaatcggtgt
     6781 ttcatttttt tcttgacgca gccgctgagt gatctccatg ccatccatcg ttggaagcat
     6841 cacatctaaa atgatgaaat caaatgattg ttctaatgct aattctaatc cttcgcctcc
     6901 atctgtggca ctggttacct tgtagccttc tttttccaaa ttgaacgtca agagcgttac
     6961 aatagaaggt tcatcatcaa caactagtat ttttttcatt caaatctcct ttaaataggt
     7021 ttcattctgt ttatatttta gcatagaatc agtacaaagg gctattttac aaaagaaata
     7081 aatggtataa taggaaggca ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat
     7141 cagtgcatgt ttaggcggtc tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga
     7201 attgatggaa ctagttgaaa ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg
     7261 attaccaatt cctagagatc ccgctgaaat cattggtgga aatggattcg atgtgtggaa
     7321 agatcaagca aaagtatgga cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg
     7381 cgcgatcaaa gcctatcaaa aattgcagga aaaacacatt gatctagtca tcatgaagga
     7441 aaaaagccct tcatgcggaa cccattcgat ttatgacgga agtttttccg gaataaaaaa
     7501 aaatggtgcc ggagtggcta ctgcttattt tattcagcaa aaaatgactg tgctctctga
     7561 tgaagaatgg ctgaaaacta gaggtgagca gaatggaaat cgaaaagacc aatcgaatga
     7621 acgcgctctt tgaattttat tcgaccttat tgacagaaaa acaaatgaac tacatggaat
     7681 tatattatgc agatgatttt tcattaggtg agatcgcgga agaatacgaa gtaagccgac
     7741 aagcagtata tgacaatatc aagcggacaa gcaagatctt ggaggattat gaaaaaaaac
     7801 tccatctttt ttctaattat attgttcgag aacaggtatt ggaaaatatg actgcatata
     7861 ttacagataa gtacccggaa gataaaaaac tacttgaata tgtacagcaa attcaagcaa
     7921 tcgaagaata aataaaagga atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct
     7981 ccagcaggcg atgggaaaaa taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga
     8041 aatgatgcgg gaaatccgcc ttgccttact agaagccgat gttaacttgc aagtagtcaa
     8101 agattttact aagcgcgtac gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc
     8161 acctgcacaa caaatcgtaa aaatcgttga tgaagaattg acgatcacac ttggttctga
     8221 aacagcagag ttaaataaat caccaaaaat accaactgtt atcatgatgg ctggtcttca
     8281 aggggcaggt aaaacgacct ttactggtaa attagccaat tatttaaaga aaaatgaaaa
     8341 tgctcgtccg ctcctaatcg caggggacgt atatcgacca gctgcgatcg accaattgaa
     8401 agtattaggt caacagctag acgttcctgt atttgatatg ggaactgaag tcagcccggt
     8461 agagatcgtc cgtcaaggga tggagttagc aaaagaaaag aaaaacgact atgtattgat
     8521 cgatacggca ggacgtctgc atatcgatga aacattgatg gatgagttga aacaaatcaa
     8581 agaattgacg caaccaaatg agattttgct tgttgtcgat gccatgactg gtcaagatgc
     8641 cgtcaacgta gctgatagct tcaaccagca attaggtatt acaggggtcg tcatcactaa
     8701 acttgatggg gatactcgag gcggggcagc actatcgatc cgctcagtga caggcgcacc
     8761 aatcaaattc atcggttctg gtgaaaaatt gactgactta gaagtattcc acccagatcg
     8821 tatggctagc cgtatccttg gtatggggga tatgctgaca ttgattgaaa aagcgcagca
     8881 agactacgac gagaaaaaag cggaagaact tgcacagaaa atgcgggaaa attcattcga
     8941 tttcaatgac tttattgaac agcttgatca agtgatgggt atgggcccac tggaagatct
     9001 aatcaaaatg atcccaggaa tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc
     9061 aaaagatgtt gaacgcaaaa aagcgatggt ttattctatg acgccagcag aaagagcaaa
     9121 tcctgatctg ttgaacccaa gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt
     9181 tgttgaagtg aatcgaatga tcaaacaatt caaggaatca agaaaaatga tgcagcaaat
     9241 gtccaaaggg gatatgaaca tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg
     9301 aaaactaggc aaaatggcga tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa
     9361 acggaaataa gatgaaaaaa acagcttcgg tacagactga aaatgtctac tgccggagct
     9421 tttctatcag caatttttat tctttactaa tacaattact ctaaacgaag aggtggcaca
     9481 gatgagatgt ccgtggagtg aacaaacaga aagtatgcgg caatatcatg atactgtctg
     9541 gggtgttcct gaatatgatg accaaaagct gttccgaaaa ttgatgcttg atattaatca
     9601 agccggattg agctggcaga cgattttaaa taaaagtgaa gcattcgatg aagcatatga
     9661 tcgattcgat atcaaaaaag tcgcaaatta tggagaaaca aaaatcagtg aactaatgga
     9721 aaacaaagga attattcgta accgaagaaa aatcgaagca gccgtccaaa acgccaaagc
     9781 agtcttgaaa attcaagaag aaatcggcag tttttcagag tatctttggt cgttttctaa
     9841 tggaaaaatc gttgattctt cttatcattc acaagaagaa gtcccaacaa caagtcctct
     9901 ttcagatgaa ttagcaaaag atctaaagaa aagaggattt aaatttatcg gaagcacaac
     9961 aatctatgct tttttagaag ctgtcgggat catcaatgat caccttgaca gttgtttccg
    10021 caaagaagag gtcgtacgta tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt
    10081 ttatggtgct ataagtttag atggctattt ggcaacgaaa acggatgatc ttcaatggct
    10141 ttttgacact ccaacaggta agcaaactac ttatgaagct ttttatgaaa caatcgatac
    10201 gaccatcatg ggcagaaaaa cttatcagga agcaaaaaaa tatttggaga ccgaagcaat
    10261 ttatccagag aaaaagaatt tcgttttttc cacccacgca caattacaat tgccagatgc
    10321 tgaggtcgta aacgaagatc ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa
    10381 agatatctgg gttgtgggtg gcggtcgatt gatcaaaccg ctattggaac atcaaatgat
    10441 tgatgaatgg tatatccaga ttacccctgt attattaggg gacgggattc ctttattcca
    10501 aaaaggaaca tatgaagcgc gttttgaaat gctagacacg acacgattcg gtgaatttat
    10561 cgaattgcat tatgtacgaa agtagaagta aataaaataa aaaaggcaac tatatgcaga
    10621 aatgtcctgt acatagttgc cttttttatt accgttatgc tttttactct gttaatttgt
    10681 tagaaaaatc tagatacgca tctaaaatcg tttggaagaa acttaatgtt ccttcaacga
    10741 tattaccatt ttcatcaatt agattcgcaa cgtttccgat atatgcttcc ggttgctgca
    10801 aggttggcac attcaaaaag acaagtgatt ggcgtagatg gtggtttgcg ccaaagccgc
    10861 cgatcccacc tggtgatgca gtaacgacta aacctggttt atgatcccaa acactgtgtc
    10921 cataaggtct tgagcctaca tctaaagcat ttttcaatac tgctggcaca gaacggttgt
    10981 attcaggagt aacgaaaacg acaccgtcta gtcccctcac ttcctcacga aaacgagtcc
    11041 attcagcagg agcctgttct ggagtttcga gatcctcatt gtaaaatggc agatcatcga
    11101 tttttacaaa tacactttcg tacccttcag gaagcaattt cgcaaaagct tctgctactt
    11161 ttttgttgta agaatctttt cttaaactac caataaaaaa tccaattttt tttgtcatac
    11221 gatttttcct ccttcaaact atctataata atcttacaaa agataagtaa ctggagcaaa
    11281 taaaaacact tactgacaaa agctacatag aagatagatt acttattttt aacaaaaaag
    11341 aagtctaaaa tcttctattt tctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa
    11401 agctttacag ggtaaaaaaa aactgttaca ctacatcttg tgaataaaac aatggaggtg
    11461 gaaataaata atggcagtta aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt
    11521 ttaccgtatc gtcgtagctg attcacgttc tcctcgtgat ggacgtttca tcgaaactgt
    11581 aggtacttac aatcctttga aagaccctgc agaagtagtt ttaaaagaag acttagttct
    11641 tgactggtta tcaaaaggtg cacaaccttc tgacactgta cgtaacatcc tttcaaaaga
    11701 aggcgttatg caaaaacacc acgaagctaa attctcaaag aaataaggtg accggatatg
    11761 aaagatttaa gcgacttagt cttaacaatc gttcgtccgc tagtcactta tcctgatcaa
    11821 gtcaagttag acgtcgtaga gtcgaaagac ttttacgagt acaatctaac agtggctcct
    11881 gaagatattg gccgtatcat tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc
    11941 gtttacggtg ttcggatcaa tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag
    12001 gaaaagtaaa taaacgtgct gtactcgaat tttcgagtgc agcttttttg ttttttttat
    12061 taattgaaga aatgattatt tggcaaaaca agcaagaaag cgtatcctta taggaggagg
    12121 gagtgaacag ccaatgagaa agaaaatgtt atttattcca tttgctctat tgttaagcgc
    12181 ttgtagttcg gttactcaag aatcagacaa tacacatcta tcaagcagta tatctgaaac
    12241 tgttaccagt ccagaaaaaa gtagtaccaa aacggcttcc accactgaac aaacaatggc
    12301 tacctcaaat aatgagaaaa agactgcttt agaccagttg aaagaacaac agccaaatgt
    12361 tcccatgcct ctagatgtac cagtctcttc aggctatctc aatatcgcag cgacacatac
    12421 aaagcaaggt tattctattt tatattacag aacagatcgt cccctcgggt tgaatgctga
    12481 cgaattgaat caagaaacgc ctattgcaac ctatctatat caatatgggt ttgcttctag
    12541 tcaagaaaca atccaagttt tgcagccatt tgagatcgac acaaatggtc agcaagtgga
    12601 cttaggttct cgaatcacag ggtatcagca aggtgcagca ggttcaagtt ttcttgaatg
    12661 gcaagaagga aactggcgta ttcgaatccg tgggaataat atagaaggcc aagatccctt
    12721 actattagct aaagaaatcg tagcttattt ggaagaaaac agtctaccag cacctgaaca
    12781 atttggtaaa attactgttg atatgggtga tacgacgaat cgagccgtag aagtaagctg
    12841 gcaagaaccg aaaaatgcct atacgatcac tcatcaagat cctatgtctg cgttgaagat
    12901 ggcagtttct atgaagaggt tgtgaaatca gcgcgctgat ctgagcccat catttaactc
    12961 caagtcacag gaagtaatca aaaatagcac gccgcatttt tgcataattt tggcttaata
    13021 ccttaaaaga aaactagaat tgcctcttca cacatgctaa aataaaacag acaaaaaaga
    13081 aatggggata taagttgact gaatacttga atgtaggaaa aatcgtaaac acacaaggta
    13141 tcaaaggcga agtccgtgtt atctcaacaa cggatttccc agaagaacgt tacaaaaaag
    13201 gaacggtgct aacactattc caagacggaa aagcacctgt ggaattaaca gtcaaaagtc
    13261 atcgcaaaca taaaaatttt gacttattga gttttgaagg acatccttct atcaatgatg
    13321 tggaaaaata tcgggatggc atattaaaag tgtcaaaaga caactctgta gatctagctg
    13381 aaaatgaatt ctattaccat gagatcatcg gtctgcatgt attagaagat ggaactgagt
    13441 taggtaaagt aaaagaaatc ttgtcaccag gtgccaatga tgtttgggtc gttcaacgtc
    13501 cgaaaaaagc tgatatattg cttccatata tcgcttctgt cgtcaaagaa gtcgatttag
    13561 aagctggagt ggtccgtgta gaaattccag aaggactgat tgacgatgaa gattgatgta
    13621 ttgactttat ttccaagaat gtttgaaggt cctatggggg aatccatcat cggtaaggct
    13681 gtagataaag gattattaga tatcaatatc tctaacttcc gcgattattc agataacaaa
    13741 catcagacag tagatgatta tccatacggc ggtggagcag gtatgctgct caaagtccag
    13801 cccatctatg ataacatcca agccattgaa aaagctgcac cggatgtcaa aaaaagagta
    13861 attcttcttg acccagctgg taaacgattc gatcagaaaa tggcagaaga attttcacaa
    13921 gaagaacact tagtattcat ttgtggtcat tatgagggat atgatgaacg tatccgcaca
    13981 ttagtgacag atgaagtttc cttaggagat tatgtcctta ctgggggaga attaggtgcg
    14041 atggtcatga tcgatgcaac agttcggctt ttgccagatg tgttagggaa tcagacttca
    14101 gctcaaacgg attcttattc aaccggttta ttggaacatc cccaatatac tcgcccggca
    14161 gaatataaag ggatgaaagt accagaagta ttgacaaatg gaaatcataa attgattgaa
    14221 gaatggcaat taaaagaatc actaagaagg acctatcaac gtcggcctga tcttttggaa
    14281 acacttgaat ggacacctca aatgacgaaa tttttagaag aaatcaagaa agaagaacaa
    14341 gaaaaaacag cagatgaagc caagtgactt tttctaaaat cttctttaca gtaggttgat
    14401 aatatgttag tattttatgg tgagtgcaaa agcacttaac tattacgata ttccgctgtg
    14461 agaaaagctc ataagaatat ttggaataag gagaagaaaa ccatgaatcc attaatcgaa
    14521 gaattaacaa aagaacaatt acgttctgac atcccagcgt tccgccctgg tgacactgtg
    14581 cgtgttcacg caaaagttgt tgaaggtact cgtgaacgta tccagttatt tgaaggtgtt
    14641 gttatcaaac gccgcggtgc tggaatcagc gaaacttata cagtacgtaa agtttctaac
    14701 ggagtgggcg ttgaacgtac attcccattg catacaccac gtgttgcaaa aatcgaagta
    14761 gttcgctacg gtaaagtacg tcgtgcaaaa ttgtactacc tacgtgcatt acacggaaaa
    14821 gctgctcgta tcaaagaaat ccgtcgttaa tcatcgttat cagacagaga aagaaccctt
    14881 ggtattcaag ggttcttttt ttattatctg tttttttata ttaacgattt ctatttggta
    14941 catttttgat taataaaaag gggcaaaaaa ggggcaacaa gttacagttt ttctatatct
    15001 tctttcaatt tttcttttac tccttttgtc acatgcaaat aaattttttc agtgatatca
    15061 ctattttcgt gaccaacgcg atcctgaatg gcatacagag gagttcctag ttccgctaat
    15121 tttgaaatat gggtatgtct aaaaatatgt gaactaagtt ttttatcaat cttcatgtca
    15181 gctttatgac ttctcaaata ggtgttaatt gctgttagtt ggaaaggagt tcctttcgtt
    15241 gtttgaaata gaaattgacc atttggattt aattctagaa gctcattgta aatagctatc
    15301 gctttttttg gtaaatcaat ttctcgcatt ccggcagctg tttttgtaga atcagatttt
    15361 ttcatatcag ctattgaacg ttctctatac atcatcgtcc catttataac tactgatgca
    15421 ttattattgg taatgtgtac atcatcttta cttaatgcga ttgcttcacc agacctcatc
    15481 ccagtcaaat atagccactg gaaaaggaga gaatatcttt tattgtgtga agttgtgaaa
    15541 tctactagtc tattatattc atcatcttct aaaaatttat ctttaatttt tatcgttttt
    15601 gactctcttt tataatcaat aactacttca tcgataggat ttttttctac ataacctttt
    15661 ttcatagcat atgaaaaaag aagattcaat ttggatttaa ttacactgac gtacttattt
    15721 gacaagtcat ctttatatat catgtcttca aaaatattat tcaaatctat tgtattaata
    15781 ccagaaacga tgtaagtctc aggtatcttc ttctttatag tgtttaatat attattcgta
    15841 ggataatatg tagattcctt aacttgtctt ttgtatataa ctaaccattc ttcaacaagc
    15901 tcatgaaatg ttagatcagg cttatgaagt gttttttcgt ttaattttat atcaattttt
    15961 ttatttaact ctagcaatgc cactttctga gtttctcgag atttattttt ataagtaatg
    16021 ctcacttttt ttctttttct agtttttgga tcaatatatc gctcattata tttatatact
    16081 ttttttccgt ttttatcagt tttagtttcg atccacattg ttttcatctc ctatttgttg
    16141 ctataatagg aatagataag taagccgatt atagcaggtt tatttttcat cacgtccaca
    16201 aactttggcg agggaggggc gtgtttttgt tagttattgt ggtaggttat ctaaagcgta
    16261 ttgtgcttgc tcatttgtaa aaccttcaaa tataagttga tcgtacaatt gcgaatctga
    16321 cattgatgcc caatcatcgt aactttccgc tttagccaaa gcttgttcgt tgtaatcgac
    16381 atcaacatta gccagcgcat aatcgattgc atcacttgga tattcttcaa actctagttg
    16441 ttcacgcaaa ccttgttcag acataccagc ccaacctaga tagctttcag ctttgctcaa
    16501 tgcatttctg tattcacgag gaacactttc ttcttgagta acttcttcta tagtagtttc
    16561 agtagtttca gtttcttctt caacgactgt agattctgct accgattctg cagaagacac
    16621 ttctgtcacg ctttctttag ttgtttcagc gacggtattt ttagcatcat cactgcctcc
    16681 ttgcgaacct atagcaataa acaccacaac aaccgctaac aaccaaaacc atacccgttt
    16741 ataaaacggt ttcttaactt tatacatttt cccatcttga cccataactt tttttgccat
    16801 ttaaatatcc ctcgcttctt gttataatat atttgtgatc tcagaaatga ggtatgagtc
    16861 cgtgttgcag cacgggcttt tttctttata acttttttag agattatagg caaaatagta
    16921 gggcataaaa atgtattatt gaattccgta tttagaaaat cctaattgaa cttcaccgga
    16981 agtcttttgc tgtgttgtac gcaatgcttc ttcttcagac attccattct gtactttcca
    17041 tgcaacaggc gacatcccgt atttgttaac aaaatcagta agtgataaag tgtcagcgtc
    17101 ttgctgagcg cttgtttgtt ggtcttctgg attctgttga gatgctgctt gttgttcttt
    17161 ctgatcttga ctgataatat tgccagcatc atctgtagtc aatccatttt cataaagggc
    17221 cacgccgaaa gcttcccact ctttgttgga ccaatttgca cgatcagctg gagttgactg
    17281 taaagtgcgt tgtttcatct gttcatatgt ttcttcttga ggtgcggttt ggattgtact
    17341 ctgactggag cttataactg ttgggctagg ttccgccgta gcttggttgg agcttgtaac
    17401 tgttgaacta gaatctgttt tagatgtaga tttgctagta gatgaactgg tttcagttgt
    17461 ttcttttgtt ttacttattt ttgtttcttg gttagaagtg gtatctgttg attcagcttt
    17521 tttattattt gaacaagctg aaagtagcag agcagtactt aacaacaaca taacgctaac
    17581 ttttttcatt ttataattcc tctttctcgt tgtaatatgt gtgctaacac gggctttttt
    17641 atataagaaa acgataagcg ctttctggaa gcccgtaaag attctttaat tcctcgattt
    17701 ttttaggata ttgatcatta tcttctttat aaagagaaac aatgagatta gcagcaaagc
    17761 aattagcttc gctttcagat ttgcttctag atgttcttgt tgatacataa tagctggata
    17821 agccacgatg aaaaatagcg tgacctaatt cgtgagcgca aatgtagaat ctttcctcag
    17881 agtctcgcag ttcatcattt aaaaagatta tcgcacgatc tctaatttct tgaaactgcc
    17941 ccttgggatt ctcgataaaa ggaacgtatt gaattttaat gcccatcttt tcacaaatat
    18001 aaaaaggatt agcggactgg tatttccgct tcaactcctc gactaaatta atcgtatcca
    18061 tctccataag ctcacatctt tttgcctttt tctttgtctt ctttcacaat atcccagaaa
    18121 gtcgctatca ggatatcttt tacgcgctgt atttgttcgg gtgtcaatgt ttccccacca
    18181 taagacatat taacatttga gtctagtagt ttatcaagtt caaccacttc ctcttttgta
    18241 gcccacttgg gaacattatt atttcccaat aaataatcag ttgtgacacc aaaataatca
    18301 gcaactttct ttaagttctc agattttggc gaggctttat cccatcttct tatttgtcca
    18361 ttagagatgc ccacctgtct ttctacttct gctatagtca catgcttttc gtctgctaat
    18421 tctttaatct tagtaactaa actcattatt atcaaccttt caaagctgaa aagaaaataa
    18481 atagcttaaa agttatattt ttggttgaca attagctttt aagctagtat atttaattcg
    18541 taagctaaat tgttagctaa ataagagcaa caaaaaactc tactaattta aaacattctc
    18601 tcggtcgcca aacttagaaa tgttatttta gaggcttttt ataagtctta tttaactatg
    18661 tattcataat agcttaaaag ctaataggtg tcaacgattt agctaatttt ttagcttaca
    18721 aattatttgt ttagaaagga gctattttta tgtctgagaa tttagactta aaaattcgag
    18781 cggagatgag aaaaagaaga atgactttca aagaactagc tgcgcttatc ggtatttcag
    18841 gagcttattt atcagatatt ctaaacggca atcgtgatgg aaagaaagca caacagcata
    18901 tcgaaacagt gaaaaaaata ttggacatcc gatagggggg gtaggcgatg acaaaactaa
    18961 aaaaacaaga ttttgtaaaa aaatataatt attctccatc tacttatcaa cgtcgaatgt
    19021 cggaactaaa aaatacagca attttctcag cggcgtatga acgggtcaca ggacaagaag
    19081 tttggatcaa tacagaatta tacgataaat ttttgtcttt caaatcctat aacaggttac
    19141 gcacaagaaa ggtaacacct aaagaattta tcgagaagca tttagttgat ttataaaaaa
    19201 cagaacattt tgagaggtga aggttaatgg gtaaattcaa cagagcatta gtattcagcg
    19261 caccgctaat cgtctatgct ttagggcttt ggggaagtag acaagcgttg ataggaacga
    19321 ttgtgtacat ggtttggatt tttatagggc tggatgaagc tgagtacaaa acaaaaaagc
    19381 cagtcgggag ggactgacta atgagtaagg acgatattgt atttctgtta attatgtttt
    19441 gtgggattgg atcaataata attaatttgt atttgattat taaagaaaaa tttttcaatt
    19501 aaaatgagtc ttttagcttt aacttaaatt ttttttgata attagctgtt cttatgacca
    19561 ttacaagttt ttcaccaggt aatatatctt tcatatctaa aacttctgaa aaagcgactg
    19621 agtagccagg agagattgct tgtgccaacg gtataggaga taaaaggcta cttttatgaa
    19681 tgcttcccat atcaaaacca ttggcgtgat aatgtgaaca attaattttc ttaccgttgt
    19741 aaagatacgt acattcgata attgagtttg gcatttttga tgggtttgca attacgccag
    19801 taagaatcat tcgattactt ctatcatgct taactaaaaa ataggagagc gtaatttttc
    19861 ttttatcttt aaataagtta tagagggata aaaaaaatcc tgaaatagaa atgattaaag
    19921 taatagcgac ttggtaatcg ctcaaaattt ttatcaagta attcaccacc tttttactca
    19981 aattatatca aaaaggagag aagaaataat gcaagaatta gtaattttga aaaataaaaa
    20041 aagacacgaa cgccggcaag catgatcgtg tcagggaaat aaactatctt tctatatttt
    20101 accacagaaa ggaatgaacc gtaaatgcta attgcaacgg atacactaga ccgcatcttt
    20161 ttaaaagacg aatacaaact gcgcaatata gatgcgtcag gaattttagt atttgatctt
    20221 tatgacaatg gaaaaattgg tatctatcaa gcaagtgata ttgaagaaac aaaccttgct
    20281 ttcgaacaaa ttgatgattc tgtggaattg gatttagatg aggcaatcct agcttttgaa
    20341 caaattgcaa aattattaaa ggaggcacaa aagaatggca actctttacc aactcagcga
    20401 gtcatatatc aaagtcctgg aactggcaga acaattggat gaagaaattc ttcgcgatac
    20461 tctcgattcg attaatgaag cgatcgagta taaggcagaa aacttagcaa aaatagttaa
    20521 agaagtagaa gggaaagctg agttaatcga tagtgaaatt aaacgtctgc aggaacgtaa
    20581 gacatcgctt ttgaacaatg ctaagagtat caagcactat ttacaagagg aaatggaaaa
    20641 gactggtaaa acgaagatta aaggtgaatt attcaacatt ggaattcaaa ataatccagt
    20701 atcggtcaat gtaatcaatg agaacttaat tccaaaagga ttctttaccc ctgtgcctcc
    20761 caaattggat aaaaagcaat tgaaggagga actgaagcac ggagatattc ctggtgctga
    20821 actcgtccaa acaaaaggtt tgaggataag atgatgaagg aggagaaata atggaacacg
    20881 aaaacgtaaa agacgctttg aaagctgcta tcgaaatcgc ggaagctaaa ggaataaagg
    20941 ttgacggcaa accagcaact gtccatgaca ttcaaaattt gacgaaagaa catctttatt
    21001 ttatcgccga cttgcttggt ttgtcggaat tgtatttaga caaataaccc cacacggaag
    21061 gagtggctag ggtggttgta ttacaagtaa tagtctgcgt aacattcatt tggtcgtgca
    21121 taccggtttg tatatatatc atgaagaagc ttcgccctga gtcttacaaa ttatacgcaa
    21181 tttacagcat aacagtagct cttgcattta cattgtttgc aatttctagg cttccgtttt
    21241 aatcgtggga atgaattttt aatgaactaa attatctata aagcaaaaaa aggagaaaca
    21301 acattgaaaa acaaactagc aaaaacaaca gcaatcatcg gactagcact aggtagcggc
    21361 gttatcggct acgcagcaag caatgcgttt caagatttgg acacgatcaa agcaaatttc
    21421 aacacagtcc tacaatacgg tcaaacgaaa tcgcaacgtg tgtcagaact tgaatcacag
    21481 ctatccaaca acactcgcac acaggagcag ctgaaagccg aaattgagca aatcaaatcg
    21541 gacaagcaga aggaaattga agccaagcaa cgagaagtcg aacaaaagca acaggagatc
    21601 gctacaaagc aacaggaagc cgatagcttg cgccaacaac tatccacagt gcaaaacgac
    21661 aaggagcagt tagaacagcg cgtgagcgag ttacggcagt atacggatca aaaggtaggg
    21721 gagttgggga aatgaaagag ttgactattt atgacccaaa tatttcatgg gctaaacaca
    21781 caattaaagt ttcttttatg atttggggtt acaaaggata tgttacttac aaagtgggcg
    21841 gtaacactaa gggtttatca ttaatagcta ttgattcaga tgatctatat gatgctaatt
    21901 tcgaggataa cccagttaat ttccgtgacc ttgatgaaga ttggttctct atggaactta
    21961 ctaacgataa aggcgacagt acacttgtag aagatgaatt cgacagacta ggtgattata
    22021 ttgtcggtgt tgaaattatt gcgcacgaac cagagtagga gagtgaatac aaaatgatta
    22081 aaagaatcac tatatcagtt gaagaagata gtggatcaac aatgcagcat accgacaatc
    22141 gaaaaacttt tttaaccaat agaaaggaga attttgatgc agattaagaa ggcgacggat
    22201 atcgaacaaa ccaagggaac atatttgatc tacggtgctc caggaaaagg taaaacttca
    22261 acggctaaat attttccagg gaaaacactg atccttgata ttgatcggac ttcaaaagtc
    22321 cttagaggtg agaaaaatgt cgatatcgta tatatcgaca acgaagatac ctggaacgac
    22381 tggggcaaca cgttggctga tttaactacc aattatgtag gtgtttatga caacgtggtt
    22441 gtcgataatg tcagcgaatt ggaacggtgt attctgtcca gtttaggcgc agaaggaaaa
    22501 aacaacggcg ttccttcgca aggcgattat caatatatgc aatttagaat ggtgaacagc
    22561 ctacgctata tgaaaaattt agacagcaac cttatctgga ccgcatggga agaaatcgat
    22621 ttgtggacgg attcggatgg atcttcttac aacatcgctt tgccgcagat caatcgaaag
    22681 attcgaaaca atattttggg attatgtgac gttgttggtc gattgatggt aaaggaagac
    22741 gatgaacgcg gatttatttt acaagccaca gattcaacct atgccaaaaa tcaattggat
    22801 aatagatctg gctgtaagca aagtgagctg attttagatg gaattgtacg actaccagaa
    22861 tgatttagtc gatcgtgcaa gacaagccta tgttgatggt tacaaggcac catgtatcgt
    22921 atcaccgtgc ggttctggaa aatcagtcat ggttgcagag atcgctaaac gaaccacacg
    22981 aaaaggcaac agagtgttgt tcttggtcca tcgtcgtgaa ttgattgatc agatcaaaga
    23041 tacgtttcaa aagatgggcg tagataatca gcttgtaaat ttcggcatgg ttcaaacagt
    23101 cgtacgacat cttaaaacca tcaagaaacc acaactgatc atcactgacg aaaaccatca
    23161 cgggttagca gcgtcctaca gaaaaatcta tgagtattat tcggacgttc cgcgattagg
    23221 ttttaccgcg acacccatcc ggttaaacgg tagtgggttg ggggatgtga acgacttgct
    23281 aattgaagga gtatcggcta aatggttgat tgagaatcat agattagctc cttatgaata
    23341 ttatgcacct aaattgatcg atacagccga attaaagaaa gcttcgactg gagattttac
    23401 aaagaaatca atggataagg cagtgaagaa tactatttat ggcgacgtgt tgaaacatta
    23461 taaaacgtta gcagaaggtg aacaggcaat agcatactgt cacagtatcg atgcaagtaa
    23521 acacactgct gaaatattca acgatgctgg ttataaagca acacatatcg atgcaaaaac
    23581 tccaaaagat gaacgtgcag atattatcga agcgttcaga aatcatgaaa ttaaaatcct
    23641 atgcaatgtt gatttgatcg gagaagggtt tgatgttcct gattgttcga ctgtaattat
    23701 gttgagacct actcaatcgt tatcattgta cattcagcaa tcgatgaggg ggatgcgtta
    23761 ccgaccagat aaaacgtcaa tcatcattga ccatgtgggt aatgtgaacc gttttggatt
    23821 gcctgatatg gatcgtgaat ggagtttaga tacaaaaaag aaaacaaaaa ctgatagtga
    23881 tttatctatc gttcaatgcg cgttttgttt tggtgcttac gaacggccaa aaggcgataa
    23941 tatttgtccg tattgcggag agttgcagcc gattgaagaa cgaaagtcag agttagaaat
    24001 cgatgaatcg gctgaattaa tgaaagtcgg agaaacgaaa attactttga attttgagaa
    24061 caataaatat tacaacatga cggaagatga agcagaaagt atagaggact tatatgccat
    24121 agcaagagct aaaggattta aacctggttg ggcttatatg gctgctaaga ggaaggggtg
    24181 gttgtaggaa tgggcggtat ttttggtaaa aaagcagaag atgttcaagg ttcaataata
    24241 aatggcgtaa caataatagg tgatactgga aaaagagata gttcaggcaa tcaaattgta
    24301 atcgctagaa ataaaaataa cgaagttttt gaatcttctc tatctaatat aaaaagaggt
    24361 gttgttaacg ggacaggcga agagagtggc tacgttaatt taaaaaaagc taatgaaaaa
    24421 taccatgttg aaggaactta cgttaaggct ttgtctgtcg gtggaacaaa aggcgttaat
    24481 tgggataaac gaagaaataa atggaaagca cgattatatt tcaaaggaga atatgtctta
    24541 caaaaggatt tcaacaccaa agaagaagca atccaagcac gacttgcagc agaagaaaaa
    24601 tatttcaaac ctatcttaga aaaatatgaa aataaaaagg agaatgatta acatgacagg
    24661 atttaactta gacttttcaa aagcgcaaca agggaacgaa atcaaagacg gtacgtacga
    24721 agtggtcgta aacaaagctg tagaaaatgc aactaaatca ggcgcagaat ttatcgatat
    24781 cgatttgatc gttcgtaacg atgtggatca accgttccaa aataaacaca ttttcgcaaa
    24841 aatctggaaa gccaaagcta ccggcaaata taacgaaggt atgatcatgg caattgcaca
    24901 agcgctacag ttggaagacg gaaaatcata taacgggttt gatgaattgc tggcggactt
    24961 tgttttgaaa accgcctcag tacgcgtgaa aactgaagag tcaaatggat ataaaaacgt
    25021 caatgtgaag tcttgggata aaacgaacac acggggcgta atgaatcacc aattcaaaaa
    25081 tggtgatgaa ccatccttcg gaccggaacg ttcaagagcg acaacggtta gaaatgacaa
    25141 tcttccattt taattgaggt gtaatcaatg tatgaaaaaa ttccaaaaga gcttaaaaat
    25201 ctcaaacaat ggtgtgttta taagctagtt tgggatgaaa agcggcagaa gcacacgaag
    25261 atcccttata acgccaacaa cgggcataaa gccaaatcca atgacgagag tacctggtct
    25321 gattttcaga ccgctctcgc tgctattgat cactacggaa tgagtggact ggggttcttt
    25381 ttcaaaccac cttatttcgg cattgatatc gataatgctg aaggtgaagt tgagcgattt
    25441 aaatcaggtg atatagaaga aaatattatc tatgaattta tcgaaagcat gagatcctat
    25501 gctgagtatt ctcaatcagg tactggcatc cacattattg ctcgcggaga attgcccggc
    25561 ggaagacgca ggaaaggtga cgtagaaatg taccagaacg gccgattctt cgttatgact
    25621 ggaaatgccg catcaaaata tttagaaata actgagccga atccaaagga tatcaaacgc
    25681 ctatatgatc gctacgttgg agatgaaaaa atcatccaat tcaaagaaga aaatccgctg
    25741 atgaataccg ttgatttacc ggttgaagaa attatcaaac gtgctgaatc gtcttcccaa
    25801 ggggcaagat tcaaagtctt tatgaacggc ggttgggaag ctttttattc ctcgcaatcc
    25861 gaagcggata tggcgttcgc caacgacttg gctttttgga ccggtagaga ctttgaaaaa
    25921 atggatgaga ttttcagagc gtctgcaatg atccgtagca aatatgacca aaaaagggga
    25981 tcggcaactt acggagaatc tctactaaat aaggcaattg ctgatacaaa ttccgtctac
    26041 aaccccaaac ggaaatctga ttttaagatt tttatcaaag accaggaaca gccaaaagag
    26101 gaaaaatact attcgtacga cgacaccggc aacgcggatc ggtttactga tatctacgga
    26161 acgttagtca aatattcgta cattgataaa tcttggtatt actacgatgg gaaagtttgg
    26221 ctacaggaca ataccggcga agttcggaaa atgatcgata cgacagtaga catcatggga
    26281 aaagaaccgt taacgatccc tgaaggcgca gacgatgaaa ccaaagaagc tttgatgaaa
    26341 gcgaaagaaa aacacgtcaa gcgttctcgc agcaatgccg gaaaaaacgc tatgatggac
    26401 gaattgaaac atcgactatc cgtattgccg gaagagttcg acaaagataa aacgctgttc
    26461 aacacacaaa gcggctatct gtctttgcat gacggtcttc tacacgagca tgagatagac
    26521 aaaatgttta cacgtgtttc aaatgtcgag tacacagaat cggtcgattg tcctatgtgg
    26581 gaagaattta ttaatcagat ttttgacaat gatcaagaat taatcagata tatccagaaa
    26641 tgtgtcggat attccctgac cggatctacc agagagcaat gtatgttcat cctctacggt
    26701 catgggtcaa atggaaagtc ggtatttttg gaaattattt ctgaattgat gggaaactat
    26761 gcgatgacta tgcaggcaca aacgatcatg gtcaagcaaa gccaatcatc agcaaacagt
    26821 gatattgcac gattgaaagg tgcgcggttg gtcacttcgt cagaaccgaa tgaaggcgtc
    26881 cgactggacg aaggtctggt caaacaattg actggtggtg ataaagtcac ggctcggcac
    26941 ctttatggaa aagaattcga gttcgaacct gaattcaagt tgtggctggc aacgaaccac
    27001 aagcccatca tccgtgggac cgatgacggc atctggcgac ggttaaattt aattccattc
    27061 actgtccaaa tcccggatca caaaaaggat aaaaatctga aatttaaatt gcagacggag
    27121 ttgcaaggga tccttaaatg ggcaatcgat ggttgcctgt tgtggcagag agagggcttg
    27181 gagaaaccaa agtcggttgt tgcagcgagt caggattatc gaaacgaaat ggatcaaata
    27241 ggtacattta tcgaaacgtg ttgcgaaaca ggtccaggac taaaaatatc aggcggagaa
    27301 ttatacaaag tttatcgtga atgggcatca gataatggcg agcatacttt cactaacacg
    27361 aagtttggca gagagatttc aaagaaatat agcaaagaaa aagctggtgg atttatggtt
    27421 tataaaggaa tcacattaaa accaagaaaa tacgataacg ttagagaact ttttaaataa
    27481 atctggaggg ttctggaggg ttgagaacga aaccctccaa cccttagaaa cgttgatata
    27541 tcagtattta tattatattt ttctttcttt ctggagggtt ttaaaaaaat aataaagtat
    27601 taaagtaaaa aataaaaagt aaattatata aaaaagaata ggtttttggg tctgaccctc
    27661 caaactctcc accaaaatct tcctaaatgc tttgatacca acgattaaac agtctggagg
    27721 gttacattga aatcagaaca tcaaatacaa gacgagatca gaattgcctt atcaaaacat
    27781 ggttgcacga tttttcgatc caatgcggga caagtgacaa cgatcgaagg gcggcaattt
    27841 tacgggatgc caaaagggtt cccagatcta tgtggacatc gtaattctga tgggaaagca
    27901 atttatatag aagtaaaaaa tgaacgcggt aagttgcgtg aagatcaaaa aaggtttgct
    27961 gaatttttaa acggtcaacc tgtactatac ggtgttgcac gatcagtgga agaagcattg
    28021 aaaattgtgg aggaatcatc atgacaacag aagaagtgat tcaaatgcgt attcgaagcc
    28081 ttcagcgtga gattgacgat ctggaacgga caaaggcagt gatggtcaat gaaacggcga
    28141 ggaaggcaat cgatttgcac atagagaatt taagaaggga aatccatcga ttggaggaat
    28201 gagcgtggat aagaaagcag caatgaaacg aatcatcgaa ctgacacatt ctgagaattg
    28261 gcaagaagac aaagaaatag ttgcagaagt ccaaaagctc ggtaaatcaa tgtggagtga
    28321 aaaaaccaaa cggagaacac cgagaaaaat tgcaatctgg catggtgacc gaattctagt
    28381 aacaggtact gctgaacagt tatctgaaat tactggatta agcaaaaaca ttatctggga
    28441 tagagctaaa cgcgagaacg ttgattcgaa aggtcgtcaa tttaaacatt gggagaagaa
    28501 ataatggagg aactaatcac aaaagtagag cagtgggcaa aagataaggg attggatcaa
    28561 gctgattcca gcaagcaaat gttaaaaacg atcgaagaga ttggggaagt tgccgcttct
    28621 ctagctagaa aagatgaata tggtttaaga gacggaattg gagacgtagt agtaaccttg
    28681 attattttag ctatgcaaaa tgatatggat ttgtacgagt gtctgaacca agcatacaac
    28741 gaaatcaaag gacgcacagg aaaaatggta gatggtgtat tcgtgaagtc gagtgatttg
    28801 gaggacagcg atgaataaaa aagtattaat tgataaacaa gcattgattg atgaattaat
    28861 gaaaatacct ggtgtgggat ctaatagtga cgctttagaa acgattaaac gtttcccatc
    28921 gtatgaaccg cagaagccag ttgtgcctaa gtttgtggcg gaactactcg actattatcg
    28981 tcagtcaaca gatgttgatt tattagcttt attgataact ttccatgatt ggtattatcg
    29041 caaaactaaa gacggtgagc atgaagaagc aattgattgg ctagttgatc atcctgaaat
    29101 ttatatgcgt gcatggctgt ttggctacga gctcgagaaa gagccgttgt attgcgtaat
    29161 aatcaatggt agatatcttg tcaaagtatt cagcaataca aacgttgtca tattagttcc
    29221 agcagatgaa ttttcacagt acgttactca aaaatttcaa ttaactgaaa aacaaatcaa
    29281 agcaattgat gaaagatact ggccgtttgc tgtgccagtg gatgaggtgg tagaaggatg
    29341 agcgacccat acattactaa attagaaaag ttgtccgaag attttgagaa gaataaaaat
    29401 aaactaacta aagataaact tatagaactg gttaggttgg ttatcagagg agctattaca
    29461 agagaaaagg cgcagcaaaa catagaaatt tttgctcgta gtgtccgaga tggattagag
    29521 aagaaagtta aacaaaatga acaacagaca ccgcagaata acaaaactaa gaaaacggga
    29581 actgaatgta ctaaagacaa agtttgaaaa agaatatgga atttcagcag aagaaacata
    29641 taaagtggca agtcagtgtg ttgctgatgc gagcggcgct attcgtaagt ttggaatttc
    29701 gatattaagt gatgatcgta aatgggagga aaaagaatga aactaaaaga cggattttac
    29761 tccagcagtc atggtatcgg cggtttaatg ctagatatgc cgacaaagaa ccctaaaaca
    29821 cgtgagaaac caaaattcaa agtcggtgac atggttcgct gtgaagcaga aggattcatc
    29881 tatccatttt gtggatatgt agaacatctc tataatcact cagcaatcat tcgtattgaa
    29941 aacacgatgg aatgtgacaa gtggttagcg aaaagcaaag agaatttagc agtagcgaga
    30001 ttggtggata tggaggttat ataaacagcg aaaaaatgta cttctaagag aaaaatttat
    30061 tttttataaa aataggaaag tatcattatg aaaaagacca cagagataaa taaacctacc
    30121 acataaaaaa tgaatatttc ttgcgattca atataggtat atttaacaat ttgtacaata
    30181 aaaaatacta taggtaaaaa agataacatg aggagcttat ttgttctaga gtgtttgaca
    30241 aagtaacaaa atgcgacgat accaatacaa acgggaatcc aaaataacag catgctccac
    30301 atagtcactc aaccatctcc tatacgcttt attatcaata gataatatca tttttcgtaa
    30361 taaagataaa aaaataagta aggcttctca ttaaaatata aaaaagacag ccgaccactg
    30421 actgcctata taagagtatt gaaataaaaa gctgctgata taataaattc cacaagttta
    30481 ttatatcaca taaaggagcg gtttgacttg atgcaattgt tacgagaggt agatttcaaa
    30541 cagacaagat gtaatgcgag agatgtgctg aagaactttc ggcgtttgga gcggatggca
    30601 ggtcgctctt tgatagatat taagtcgccg attattacgg atatgccgaa ggcgccgaag
    30661 cacggcaata aggcagaaga cgcaatcatt cagatgatgg atatagaagc ggagagagac
    30721 gcgattctag cagccttgat ggctcttagt ctgattagtc gtcagatact ctactatagc
    30781 ttttgtgtgc cagatagctt ctcaaactac agaattagcc gtgaagtggg ttattcagaa
    30841 agaagtatac aacggatgaa gtcggaagct ctaatagagt ttgcagaagc atataaacac
    30901 ggaagaataa ttgcttataa ataatttggc ggctttttgg cggaatgatg gcggttttta
    30961 gctatttacc agtgatatta tggtagtgtc gaaagattag tgataggtct aagacaaaat
    31021 aataataaaa ggaacatcgt tttattattg tttcacaatt aagcttcgat agacagcaac
    31081 ggaaatatta agaataagga tgtgaattt
//
LOCUS       DAZKOW010000021        31041 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000021 DAZKOW010000000
VERSION     DAZKOW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.021"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..1174)
                     /gene="mnmA"
                     /locus_tag="ACG912_000996"
     CDS             complement(53..1174)
                     /gene="mnmA"
                     /locus_tag="ACG912_000996"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFQ0512333.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            1578..2177
                     /locus_tag="ACG912_000997"
     CDS             1578..2177
                     /locus_tag="ACG912_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0512334.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(2315..3601)
                     /gene="efmA"
                     /locus_tag="ACG912_000998"
     CDS             complement(2315..3601)
                     /gene="efmA"
                     /locus_tag="ACG912_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFQ0512335.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3872..4216)
                     /locus_tag="ACG912_000999"
     CDS             complement(3872..4216)
                     /locus_tag="ACG912_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFQ0512336.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(4353..5498)
                     /locus_tag="ACG912_001000"
     CDS             complement(4353..5498)
                     /locus_tag="ACG912_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0512337.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(5678..6652)
                     /locus_tag="ACG912_001001"
     CDS             complement(5678..6652)
                     /locus_tag="ACG912_001001"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0512338.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(6732..7607)
                     /locus_tag="ACG912_001002"
     CDS             complement(6732..7607)
                     /locus_tag="ACG912_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFQ0512339.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            7737..9644
                     /locus_tag="ACG912_001003"
     CDS             7737..9644
                     /locus_tag="ACG912_001003"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFQ0512340.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(10185..13463)
                     /locus_tag="ACG912_001004"
     CDS             complement(10185..13463)
                     /locus_tag="ACG912_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFQ0512341.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(13734..14279)
                     /locus_tag="ACG912_001005"
     CDS             complement(13734..14279)
                     /locus_tag="ACG912_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0512342.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(14291..14983)
                     /locus_tag="ACG912_001006"
     CDS             complement(14291..14983)
                     /locus_tag="ACG912_001006"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFQ0512343.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(15002..15286)
                     /gene="macP"
                     /locus_tag="ACG912_001007"
     CDS             complement(15002..15286)
                     /gene="macP"
                     /locus_tag="ACG912_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFQ0512344.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(15299..15862)
                     /locus_tag="ACG912_001008"
     CDS             complement(15299..15862)
                     /locus_tag="ACG912_001008"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFQ0512345.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            16090..16752
                     /locus_tag="ACG912_001009"
     CDS             16090..16752
                     /locus_tag="ACG912_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFQ0512346.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            16876..18072
                     /locus_tag="ACG912_001010"
     CDS             16876..18072
                     /locus_tag="ACG912_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFQ0512347.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(18173..19549)
                     /locus_tag="ACG912_001011"
     CDS             complement(18173..19549)
                     /locus_tag="ACG912_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0512348.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(19586..20452)
                     /locus_tag="ACG912_001012"
     CDS             complement(19586..20452)
                     /locus_tag="ACG912_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0512349.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(20541..21566)
                     /locus_tag="ACG912_001013"
     CDS             complement(20541..21566)
                     /locus_tag="ACG912_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0512350.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(21584..22258)
                     /locus_tag="ACG912_001014"
     CDS             complement(21584..22258)
                     /locus_tag="ACG912_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0512351.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(22442..23335)
                     /locus_tag="ACG912_001015"
     CDS             complement(22442..23335)
                     /locus_tag="ACG912_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0512352.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(23409..25106)
                     /locus_tag="ACG912_001016"
     CDS             complement(23409..25106)
                     /locus_tag="ACG912_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0512353.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(25099..26412)
                     /locus_tag="ACG912_001017"
     CDS             complement(25099..26412)
                     /locus_tag="ACG912_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0512354.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(26412..30353)
                     /locus_tag="ACG912_001018"
     CDS             complement(26412..30353)
                     /locus_tag="ACG912_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0512355.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(30462..30920)
                     /locus_tag="ACG912_001019"
     CDS             complement(30462..30920)
                     /locus_tag="ACG912_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0512356.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         8817 a         6796 c         5485 g         9943 t
ORIGIN      
        1 gaaaaggtgt ttcttttgtt agccatagct gttttgattc atttaatatc gcttaaacgt
       61 attgtaaaac ttttgtttct tgatatgcgt ggtcgatcaa accaccaccc agacattcca
      121 ttccatcata aaatacaact gcttgtccag gagtgattgc acgtacaggt tcgtcgaata
      181 cgacttctgc acgattatca tccaataagc gaactgtcac aggaacgtct tgctgacgat
      241 aacggaattt cgctgtacac ttgaattctt ttggcattgg ttcattcgtc gtaaagtgga
      301 tttcacttgc atccaagctt gtggcataca atgcaggatg atggaaccct tgccccacat
      361 ataatgtgtt tgtcgctaga tcttttccta caacaaacca gggttcttgc gaatctcctc
      421 cgccaccgat ccctagtcct tgacgttgac caatcgtata atacatcaag ccagcatgtt
      481 gtcctttgac ttcaccatca agagtgacca tatttccttt ttttgctggc agatagttgc
      541 tcaaaaattg tttgaagttc ttttcaccaa taaagcagat tcccgttgaa tcttttttct
      601 tagcagtcgc aaggcctgcg cgttcagcaa ttgcccgtac ttctgatttt tccatgccgc
      661 ctagagggaa catcgttttt gaaagctgtt cttgtgataa ttggctaagg aagtaagttt
      721 gatctttatt attgtctact ccacgaagca tgtggaccac gccgttttca tcgcgagtga
      781 cttgcgcata atgtccagta gctacataat cggcgccaag atccattgca taatccaaga
      841 atgctttgaa tttgatttct ttgttgcaca tcacgtctgg gttaggtgta cgtcctgcgc
      901 ggtattcggc taggaaatat tcaaatacac gatcccaata ttctttttca aaattcacag
      961 agtaataagg aatcccgatt tgagcagcta ctttcgctac atccttataa tcttccgtgg
     1021 ccgtgcatac accgttttcg tccgtgtcat cccaattttt catgaatatt ccgatcacgt
     1081 cgtatccttg ttcctttaac aaaagtgctg ttacagatga gtcgacaccg ccgctcatcc
     1141 ctacgacaac gcgtgttttg ctgttgtctg tcatgatatc accatcattt caattagatt
     1201 ctgaaacatc tacgagttga aggtcgcaac ttttcagtat tttacattat acgtcatatt
     1261 tctgaaaatt tcatccctta attatcaagt cgtaaaaata ctgttctact ggacctattc
     1321 agtacacaaa catgatagtc aaccaaaaaa tcactcatct tttgagcatt cgatataaaa
     1381 aattggcata ctcataaaaa gggccagcct ttcggcaata aggaataatt ttataaattg
     1441 tgaaaaacta tttttcaaaa ttgcttctta tcgcttaaag ctgcacgtct catttacaac
     1501 cttttctttt tagatgttat gagttgctca gataagtgat tttgtttact ctaagaaaaa
     1561 agggaggcaa aagatacatg aattacttta tcagtgatat gcactttttc catgaacgct
     1621 tattaggaaa aaacgacttt gctccgcggc tctttgcaaa tgtggatgaa atgaaccaac
     1681 agttgatcac tagctggaat gcagtaataa aagaaaccga ccatgtttat catctaggcg
     1741 accttgctat gcatccgcaa tatgaaaaag caaatgcaga aattttggaa ctggtcactc
     1801 aactgcaagg aacaatccac ttgatcaaag ggaaccatga cagtcgcgct tttttccgtt
     1861 atttagaaaa aaatgatcct aggcttactg acggaagtcc caaattttat ttttatgatg
     1921 taggtgccat cgttaaattc gaccatcaac aatattactt gactcattat ccgatgttgc
     1981 tcgggcctaa cgacactatc cgcaatcttc atggacatat ccatcattac agcgtaccta
     2041 tcgagaacga tgtcaatgta ggcgtagacg caccggaaag agaactctta aaggaaaaac
     2101 taccatttgg cactccgtta tctgagcaac aaatcaaaga aatctatgaa gcaaagagga
     2161 agtacgatag gtcgtgaaaa aactgcccta tatcaagtat taaacaaata aagataatta
     2221 ctcatatttt tcatttgagt aggcttattg ccacaggaac attaggatca gaaatggagt
     2281 tgtaacaaga cttctgtcac aactccattc catactattc gtctttttgc tcatggtgtt
     2341 cttttaccag ttttttctgt aattctttat cataatttcg ggcagctgac gttaaaaata
     2401 aaacgattcc gcataataaa atcccgattc cggaaaacaa gaacattttt tctaccccta
     2461 aacgatcagc taatggtcct gcaaataata atccaattgg acctgccagg ttcatcaaag
     2521 agttgaagtt ccctaagaca cgacctaaaa cattcgattc ataactctgc tggatcattg
     2581 ccatcagcag tgtattgaaa tatggcgttg cacaacctgc tcctgcattc aagataagaa
     2641 agtacaagaa cccttgacta tcgtttggaa ccaatccgct tgcaccgatc gtcaggccca
     2701 taatgatgta ggctataata ataggtttca ttcggtcttt ccatgttcct ataaaactga
     2761 tcagtgcacc gcctaagagc attccgactg cgtagactac ttccactgct cccgcctgtc
     2821 caacggaacc accaaagtaa tctagcgtca tcaatggata cagactgatt gctggcatga
     2881 acaacagcat cacaaatgca ccattgatcg ttatgtacca taacccttta ttatccatca
     2941 attgctgcag cccaaatttt gcatctttaa aaatagtaag cggaacttcc aatatttcag
     3001 gagaaacttt tgggatcttc acaaaaatca ataagccgac accaaaaact gcccccaata
     3061 catctaataa aataaggtaa ttgacaggaa cgactgaaaa caaagccgca cctagagctg
     3121 gcgcaatgat gaaattcgct gattggacca ttcctaactg tccattcgtc cttgttatgt
     3181 gagaagatgg aacgattgtc ggcataatcg actggatcgt tggcatttgg aatgtttgtg
     3241 cgaccgaacg cataaacaaa gacacaaaaa ctagccaaat cgggaaggat tctgaaatcg
     3301 ttccgactac tgctaaaata agcgcgaaaa tcgctacaat aatatccgtg acaatcaaaa
     3361 gagctttttt atcccagcga tccactagcg gtcctacaaa tgggcttagt aaaaccatgg
     3421 gaatcattcc tagtaaagta gcaaaactca aaattgtcgc cgaaccggtt tctcttgtca
     3481 aataccagat gatcgcatat tggacgacca tactagtgat tcctgacaaa aactgtcccg
     3541 ataaaaagaa caaataattt ctcttccaat tcgttaaaac gaccgactgt tcattttcca
     3601 taaacatccc tcttttcttt ttcattttgt atagcgtttg cacaacaatc attatacggg
     3661 attctttttt caaaaaatac ctttttccct aaataacaat cgtttcaatt taaatttctt
     3721 tacttagaaa caataaatca gcaaaatatt aaaaataaca acatgtgtta aatttaaaaa
     3781 taaaaaagac aactaatttg cgtcgtcctt acaaagttag tcatctaaca ataggccgtt
     3841 tcaaaactag ttgtcttttt atatccacag atcattcgac tttttcgaat aacccacggc
     3901 taatcatccc atccatcaga aaataaaatt tatcttggct cattttctgt tttggatctt
     3961 tgaaaatatt gactgctttc aatccatttg ctgtagtgat cgacattttc aatgtttgaa
     4021 tatcttttga tacagtgatt tttaatttga atccttcttt gctttgcggc gtggcatcta
     4081 atggtcggat caattgataa ttgcctccgt ttgtttctgt aaatccatta tcgcgcaatg
     4141 tatatctttt tgcatcagga tgcaaacgat agctaattgg tgcgcccagt accgttgctg
     4201 ttgtttcaaa tgccatgatt ttcacctcga taaaattagt ttctctttat tataaaagtt
     4261 tatcctgatt tttactaggt ttttcagtta agttccctaa aaaatgaagg cgttctctca
     4321 gacatctgtt catataagaa tgacttcact agttattttt tgacagcacg aactaattga
     4381 tcaaggaaat actggatgtc gtcttcggta tttcccaaac caaagctgat acggatggat
     4441 tcttttagta tcggtgtatc ttctccatac attgcttcta atacgtgtga aggctcgata
     4501 tttcctgctg tacaagctga accagtagaa atagcgaccc cattaagatc aagcttcatc
     4561 agtagcaaat catttgcttc gccaattaag cgaagattca acacatgagg gagtttattc
     4621 gttaaatcac catttacttg ataagggatt tcgtttgctt ctaatgtatc aagaatcaat
     4681 tgctggaatc ctttatattt gttgttacgt gcttcacgtt cttcaggtga cagcagttca
     4741 actgcttttg ccatacccca gatccctgct aaattttctg tacctgcacg ccgcttttct
     4801 tcctgctccc cgcctaaaag caatgcaggg atatttactc cgtctcgttt atataagaaa
     4861 ccgacgcctt ttggcccatt gattttatga gcagagatac ttaataaatc gattcctaat
     4921 tcttctggat ggatcgtttg gtttccgtac gcttgtaccg catctgtatg gaaataagct
     4981 ggatgatccg ctaaaagttc accgatttct ttgattggca tcaaatttcc gatttcattg
     5041 ttcccgtaca tcacagagac caagatcgta tcttctcgta acgctttttt gacctcttcg
     5101 atcgacagat tccctttttc atctacaggc agatatgtaa cgtcaaaccc ctttgtctct
     5161 aaatatttca tggtattaag aacagcagga tgttcgatag ctgtagtgat tagatgtctg
     5221 ccttcttttt gacgcgagaa cgctgtttct aagatagctg tattatcccc ttctgttccc
     5281 ccactgttaa agatgatctc gtgaggtttt gcttgcagac tgtctgcaat gatttgtctt
     5341 gctgtttcca atttttcatg cgcgtggcga ccaaatccat gtatgcttga aggatttcca
     5401 aacgtgtctt gcatgattgc cgtcatcgct tcaattactt gtggatgcat gggggtagtt
     5461 gccgcatgat ccagataaat agttttcact tcattcatcc cttccaaatt attgctaggc
     5521 acattctagc ataaaatagc gaacaaacca aagaagagct catttttgtc tagaggacgt
     5581 ttcatgggca tagaaaaaag gagctgtgac aaaagtcttg tcgcagctcc ttgaagctgc
     5641 tcacttctgt cacaacttct ttccctcttt tttttgttta ttcgaattct ttttcttcca
     5701 gacggaataa cggactcatc ggtgtatttt catggatgcg tttgaccgct tcacccatta
     5761 attctgaaca tgtaacgatt gttaagtttt ctggatgacg attttcttct gtgaaaacag
     5821 aatcagtgat acaaatatct ttgattggtg cattgtctaa aatacttttc gcatgatcgg
     5881 acaataatcc atgtgacgca caggcatata cttcttttgc gccgttttct tttaatacat
     5941 ttgctgctcg tgcaagcgtt gctcctgtgt tcaagatatc atctaccatg atacatgtct
     6001 ttccttgaac atcaccgatc acataaccac cctcagttga agcatcatct gcatgatcta
     6061 cgatggctaa tgtgctgttc aaatattctg aaagactacg tgcacgctga acaccgctgt
     6121 tttttggcga aacaactacg atgtcatctc ccgtcagacc taattgacgg taataatgag
     6181 caaacaacgg catcgtaaat aaattatcta caggaatatc aaagaatcct tgtacttgaa
     6241 ccgtgtgcag atctaaagtc agtactcgag ttgcccctgc ttcttcgatc aagttagcga
     6301 tcaatttcgc agtgatcggt tcatgaggtt tcgccgtgcg gtcttgccta gcatacccat
     6361 agtaaggcaa tacgacattg atcgttttcg cacttgctcg cttcattgca tcgatcatga
     6421 tcagtagttc catatagtaa tcattgacag gtgcattcgt tgattgaacg atatacacat
     6481 gatcccctcg aatactttct tcgatattga tggaaatctc gccatcacta aattgcttca
     6541 cgacgctttt acctaattct gttccgaaca ctttcgcgat tttttcagct aatggacggt
     6601 tgccattcaa actaaaaatc ttcaacgtgt catcctgata cttattactc atgtagtcct
     6661 ccccaaaact tctctattct tcgatagttt aacatatttt tccaagattt tctgttaatt
     6721 tcagcaaatt cttaaggaag tgaaactgtt ccgttactcc aactggcagg aatgacttta
     6781 tgataatcgc cccagttgtc gtgtactttg taataacgtt ctcctgtttc agtttcaaaa
     6841 taagcgtacg ctgtcaccca atgattgcca tatgtactgc caagtacttt caaaatacca
     6901 atcatcacag gtttgccttc acgtatacgt tttgtagctc gctgccatga acctagaaga
     6961 gttgctcgtg cacgctcatg attgccattc ttctttaaaa aactagaaat ccctgtacta
     7021 acctgaaaag gaatcgtagg aagtcctagt ggttggatct ggctacgcaa tgccttcgtc
     7081 aaaaccaacg aatcagccgt atttttcttg cgaatctcat ttggaagcat ccacccctta
     7141 cgatagtctt gataataggc aaggagaact gctgccgcgt aagtaccaca aagatagcct
     7201 gagggggtag cccatttctt atacctcaaa aaacgttctt ctggcaatcc cacccagcaa
     7261 tttgcgagtt cctttcctga ccttttttct tcgatcaaaa cgggaatatc ttcttttctg
     7321 ttgatccatc tagataactc gattggctct tcattgatca gcttccattt tttacaagaa
     7381 agaccctctt gataagtata ttttgctgga gaatattcta ccggatgtcc tgattgatcg
     7441 ataatcacat aactttgatc ttcaaaaacc agagctaaaa aaagcaattt actttttctt
     7501 gactgcagct gctgagtgct ggctaatttt ttaatttttt tatctacttt gaaatataaa
     7561 ggtttatcca ctacttgatt cagttgggat cgcctccttt ttttcattat aagtgaagga
     7621 ttgaaaaaaa caaatcagtt tgattgttcg aattttcaat tttttatgtt ctatttgtta
     7681 attccttcat tttgtaagat aatgggcaca cgaaacaaaa aaggagcgat cgatgaatga
     7741 acaaagaacg attgaaacaa gatttctatg aagcagtcaa tgaagaatgg caaaaaacag
     7801 caaaaatccc tgcagataaa ccagctacag gcggtttcca agatttagtt gacgggatcg
     7861 accaactttt gatgggagaa acagacgatc tgcttgccca tccagaaaaa gtgtcttcag
     7921 aaaaaatgaa acatttcctt gcatattatc aactggcaaa cgattatgac cgaagaaatg
     7981 agttgcatgc tcaaccgctt caacctataa tcgaaaaagt tgaatcattg cagtctttcg
     8041 acgaattgaa taagcaattt ccagaatggc tgttggatac attaccattg ccatttaatc
     8101 tggatgtaga tgcagatatg aagaatgcgc aaacgaacgc cttctttgct tatccgcctt
     8161 ctttattcct tccagataag acgtattatg catcagacca tcccaacggt gcacaactaa
     8221 taggcgtttt ctttgatatg gctgttcagt tattagagct tgccggcaaa gaaaaaagtc
     8281 aggcagaagc aatcacggaa caagcagtgc aatttgataa attgattgct cctcatgtaa
     8341 aaagtgcaga agaaaacgca gattacagca aaatgtataa tccgcaatct tttgatacat
     8401 ttattaaata cacagataaa ctggacttga aaaaacttgt cacgggtttg attgggacaa
     8461 ctccagataa agtgatcgta actgatccag tgtacttcga tcatttgaat gacatcctga
     8521 cagaagctca ttttccgtta ttcaaaagct ggatgatcat taaaacagta cgttctctta
     8581 gtggttatct atcagaagaa ttccgccaag tcagcggaat ctattctcgc gtattgtctg
     8641 gaacagacga agcaatgcct cagaaaaaag cagcgtatta ccttgcctct ggacaattcg
     8701 atcaagtagt tggcgattac tacggaaaaa aatattttgg cgaagctgct aaaaaagacg
     8761 tagaacaaat ggtgaaaaaa atgatcgccg tttataaaaa acgtctagaa gaaaatgact
     8821 ggcttgggga tgcaacaaaa gaaaaagccg tcataaaatt aaataactta ggtatccaag
     8881 taggctaccc cgataagatc ccagcattat tcgaccagtt caaaacagta cctgcagatc
     8941 agggtggtac tttgctttca aatactcttg ttttcagcaa actcacatta aaagatcgtt
     9001 tcagcaaatg gaacaaacca gtggatcgtt cagaatggga aatgagtgct gacacggtca
     9061 atgcttatta tcatccattt cggaatgtga tcgtctttcc ggcagcgatc cttcaagcac
     9121 cattttacag tctagaacaa tcaagctctg ctaattatgg tggaatcgga gctgttatcg
     9181 ctcatgagat ttcccatgca tttgataata acggcgcttt atttgacgaa tatggcaact
     9241 tgaacaactg gtggacagaa gaagatctta cacaatttca aaacaaagca caagcaatga
     9301 tcgaagaatt cgatggtctg ccttttgctg acggaaaagt aaacggaaaa ttaaccgttt
     9361 cggaaaatat tgctgatgca ggcggtctaa gctgtgcatt ggaagcagca aaaacggaat
     9421 cagatctttc tatcgaggaa ttctttgtca attgggcaac gatttggcgt acaaaagcaa
     9481 aaaaagaata ccaacaactt ttactgcaaa tcgatgtcca tgcccctgct aaactccgag
     9541 caaacgtgca accaaaaaac cttgacgaat tctatgaaac atttggtatc acggaacaag
     9601 acgctatgta tctagcgcca gaaaaaagag tgcgaatctg gtaagaggtt gtgccagaag
     9661 tggtcagctt caagaaataa gaaggaattt gggaaaatcg cttctcaaat ttttgtgaca
     9721 tttcggctta tttttcgaag aagttacttc tggaatacca tgtattagag gttgtgaaaa
     9781 agctgcttag tttcaagtag taagaaaaat tttgtgaaaa tggctttcca catttttgca
     9841 taaatttgac ttactaccga gaaactggct tttgaacacc gtttatcgag aggttgtgaa
     9901 atcagcgtcc tgacctgagt attagagaaa aaaacggaaa aatagttcct catattttgc
     9961 cgtttgttga gctaataccg agggtcagct gattgaacac cgtttagtgg agagattgtg
    10021 aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaagcagcaa ggagaacctc
    10081 atctataaaa agatagaagg aagtgagaca tctattggtc tcatttcctt ctatttttac
    10141 tttcctcagt caattgtttt acatcataaa aggagaaatt ccacttattt ttttcgctgt
    10201 ttcatgacat aaacaactac accgattaac aaaattcctc cagctaatgc tagcaacggg
    10261 ttattaaatt ctcccgtttt tgggagtcct tgagtgccat ttttttcact ttcttttgct
    10321 gtcctatccg gatgagaagg ttttccattt ggttttccat ttggtgattc atttcctgga
    10381 ttttctcttt ctggtttgtc atttcccggg ggcacatcgg cagtttctcg cacagtgatc
    10441 gctttttgct gggtaacaga ttctgtatga gcagaccatt tttccccatc aaatatttcc
    10501 gaacgataag tgacttctag cgtatattct ccttttgaag gtgctgaaaa aatagcggta
    10561 tgattagaaa attgagcact tgactgtgtt tcaacgattt ggatcttttc tgggaaataa
    10621 cgcatatctt caaagacagg cgttccttga tttggtgtga aagttgacac aaaaactgta
    10681 aaaggcttca gttcaatgat atttccttct gcattctccg ccacaccatt tgcttcttgt
    10741 ataataatcg gattattttg ttcatttggt tttgtctgat ttggttcttg tacgattgca
    10801 tactctggat attccgaaga cggtgaggtt tctccgtcaa aaatagcacc tacagataat
    10861 ggaagaccat ccactctttc cttttctgtt tgacgattcc ccggtttaat ctgcatcagt
    10921 ccatgaacat ggatacttcc attttcatag cgcgtaacag gctgctgacc ttctttcgaa
    10981 catgtattta gagactcgat ccaatctttt gatatggtat gtccaagagt attttctgat
    11041 tgtttctctc gttcattcca atagtagttc aacgagtggt agatttgatc ttcgttttga
    11101 ttcaatgggc ggatctgctc tctcatctca agattctctg ttcggaaaga taagatacca
    11161 gaagcatggt agtctgtgtt tcctgctgaa gaggtataaa gagccacatt atatgaaccg
    11221 ttattaaaac ttgtactgct attgacgata atatctgttc cgctattgga atcgattcct
    11281 ttcgcaagat tttcgaaaga caaactattg atcaattcat gagggcccgt taaagaagaa
    11341 ccgcccattt tgaatccatt accgttacct tctccctcaa ttcctggtac ccagccgttt
    11401 cgataagcaa ctgagttttt gatgattact tttccgatcg aacctgtttc atttttagca
    11461 aaaaggtccc aaccgtcatc tgcattatga tacgcgatac aaccgtcaaa tacatttcct
    11521 tctcctattg tcagtttggc cgcaaatcca tcggcatctt caaatcctgg atcggcattt
    11581 tcaaaagatg tacagttctt gactaagtta tgcgcgggcc atgcttcgaa aggatcattt
    11641 gagctaccgc tgatttggat tcctgtattt ccattccgat aagcatgaat ctcttcgatc
    11701 aaattatgat ttccagaaat ctgtaatcct ttttgcatag ctgcactatt tgtaatgtca
    11761 aaccctcgta aaccccagta gtgactgaac agtgtcatcc catttccagt tcctttaaaa
    11821 tcaagtacag gacgcttgtt ttctggattt ttctctgcaa tcaactgaat cgggttttct
    11881 cttgtcccgt taacgccttt tggaatcgtc aatcctcttt cgaaggaata agttccaggc
    11941 gccatgacga ttgtctgccc agcatatgcg tatctcaatg cttgtgtcag agataatgga
    12001 ttttgtctgc ttccgtttcc tgcatctgac ccttctggtg ttacataaac tgtcgaatat
    12061 ttaggtttac ggtagtccac tgtatgaaga atctcttttg tttcataact agtcatctct
    12121 acgtattctt ctggcggata gtttttatct ggcgtaaaac tgaaagtaaa ttcgttcgtt
    12181 ccttccatca gttttgtctg ataatcaaat tcccgattcg cagtgatagc caagtttttt
    12241 gcgttcttaa gcacctctcc tgttgcttta tttttaattg tcaataatcc gtcagcattt
    12301 gctctaaagg acatagtatg atcttggttt cctgaatgat tactagaggt cacagtgtaa
    12361 atcggatcaa tcaagcgagt aggtctttct tgcccaggag gatctgtcgc aggatctgag
    12421 taagcaactt cgattccttc taccttgatc gtcatattcc ttgctgcagc aaatcctgca
    12481 taaataaccg attcatctga agcgtataat ttttcccagt catacatgat gtcagaagcc
    12541 atttcttctc ctgtttcagg atcgtaataa tatgcctcta aaccagtatt cgtttttttc
    12601 atctctacgt ccagtttagt aatcgagttc gtggccggaa acagattccc atgaccgttt
    12661 ccgaatatat tataagaacc tgtttctttt tccaagaacc ctgctgaagt ctctaaaggt
    12721 gttgtttcca ctgagacact cccaggagct ggcgcagctt gcggatcttc agatgatatt
    12781 cctgtgattg cccgtgtccc caatccaagt cgcatcgtat atttgctgcc gctcgtgttg
    12841 accacttctt gtctatccga gtcccagaca tattcgatcc gactgcctag tatggcaaat
    12901 gaatttgaat aaaatgcctg cccaaacaaa tgattttttg gtactgcgtc tcttaccatt
    12961 aaagcaaatc cttcttgggc attcgtatag gtccaagatt ctatttcgat cgtcgcccgc
    13021 atcctaaagt tgatgtcttt ggggattgct tgataataat aggtcattcc atctgatccg
    13081 cttgactgga attttccgcc gttattcgtg gaagagagcc gcacgccgtc ttctaatggt
    13141 tcaatggcat acctactagc acttgtgctt tctccgaaaa caaccccttt ccactcttca
    13201 tttgcttctt cagattgctt ttttgtcttt tcattttctg attctttcat cataggatca
    13261 tttccttctt ccgtatccat atcttcacta gttcttggta tttcctccat tgttgacatc
    13321 ccttcaatgt tcacgtgtgc attttcttcg ttcacagact gttgttcgtt agcagcaact
    13381 tcaacgacag aaattaaaac cggggtactg agcataagga gattcatcat tagcgcactg
    13441 tattttatac gtttttttaa caagtgtatt cctcccgtaa caaattagaa agcgtttact
    13501 taaagtatta cctaatttat ttttactgtc aataaaaaac aaaattataa taagtgtatt
    13561 ttataaaaag aaaaaatata acttaagttg ttgataataa agagatttat taacggaaaa
    13621 agaataagtt tttttaaaat aacgtataaa aaagagcata aaacaaagtc gtttgtttta
    13681 tgctcttctt ttattatttt gtgcttttta ttttatcaaa tcactttgtt tttttataaa
    13741 atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc attcatgtcc aacgggatcg
    13801 aaacttgcca cggaatcttt gaaaatatgg agtttgtatc ctagattata cgcatcgact
    13861 gctgtatgca acacacagat atctgtacat acgccggtca aataaatatc tgtgatctgg
    13921 cgttctcgca aacggatgtc tagatctgtt ccactaaaag cggaataatg ccgtttgtct
    13981 atccaataaa cattttcctc ttgctcatag tcttggtata aactttttaa agagccgtac
    14041 aattcccgtc cacccgttcc tatcacatta tgcggaggaa ataagcggtt ctccggatga
    14101 aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga caaaatcctt ttgatcaata
    14161 aacttctttg tatgagcagt cagctttttt tcaattactt gtcctgctgt cccagtagtc
    14221 agctttccat cctctgcaac aaaatcatat gtgtagtcaa tcgagatcaa tgcgttcatc
    14281 ttagtcctcc ttatacgaga tgtttgacaa attcgatcac catttctgct gatcgtttgc
    14341 ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga gtgatctgct gtgtcgctca
    14401 ttgcacgtac gatcaaaaat gggatattga actgacgagc tgtttgtcca acagcggctc
    14461 cttccatttc gcaagctaat gcttcaggga aattcgttaa gatttctttg attttatctg
    14521 gcgagtcgac aaatgtatca cctgtaacga tcaatccttc ttttgcgttc aaattcgttg
    14581 cttttgctgc tttaaccatt tcactgcgca aatatgtgct tgcttcataa tatagaggca
    14641 ttcctggtaa ttgtcctggg ttgtagccaa atcctgttgc atctgcatca aaataagcta
    14701 ctttgtctga tataacgatg tcacctactt gtaagccttc accgattccg ccagctgaac
    14761 ctgtattgat caccatgttc acgccgtatt gttggatcaa cagacttgtg gtaatagatg
    14821 ctaagacttt tcctataccg gaacgtacga cgatcacttc gtggttacct aatgaacctg
    14881 aaataaataa agcgcctgcg cgttcccatg ataatggttc gcttaggttc tctctcaaaa
    14941 tttttacttc ttcttccatt gcgccgataa taccgatttt catcttttta attccttctt
    15001 tctatataaa tgcgattgct aaaaaaacca cacataacat taggataaca ataataagag
    15061 atttcatcaa aaaagagttc catttggtcg tcttttcccg ttcgctgatc cttgttttcg
    15121 tgattggttt attctttttg ctttctttac gataaaagct ggcaatcttt ttttcttctt
    15181 gctgataagc tgtttctgct tttcgctgtt tttttaatga ctcgcttgct tgtgcttgtt
    15241 gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt ggccataagc cgatccctct
    15301 actttccttt ggtcaatagt tcccagtatt gaatcgcata aatcgtcttt gcatcacaaa
    15361 tcaactgatc tttcatcgct tgttgcgcct cttcaagagt tagatgatac aactccagta
    15421 cttcgtcttc atcttgtgct aaaggattct cgaccttctc taccccttgt gcatgataaa
    15481 tatgaaggac ttcatttgca aaacctggtg aaagatacat cgaagtaaga tggctcagcg
    15541 acttcgcacg atagccagtt tcttcttcta attctctagc tgctgtcatt tcgggatttt
    15601 gtccttctcc tggatcgatt tttccagctg gaatctccaa aatcactttt tctaaaggtt
    15661 ttcgaaattg tttgacaaga agcagacgat cttgttcgtc aaaagcaatg atcccgacac
    15721 cgccaggatg aaataccagt tctctcttcg aagttcctcc atctggcaaa cgaacttcgt
    15781 ctacagcaac atcaataatt tttccgctat agatttcttt gcgactgatt gttttttctt
    15841 caaattgctc attgttcagc atatcttctc ctgctttctt tgtaatcata actgcttttt
    15901 tttcattttt caatttttct caaaaactcc ttattagttt accatagata aaccttagat
    15961 tttctctaaa tatagaaaat ccttctttag tccgatactc tttctagtag tccgtatgct
    16021 aaaataagta tgtaatcgaa taacgaaaag aattcaatac tcaacgataa atgacatgga
    16081 gggtttatta tgataaaacg aaattggccg tggcttctag cattggtctt gctatttctc
    16141 tttgtccgaa acaatagttt ttcttttgtt ctaggacttt tattgatagg tgtagtttta
    16201 gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa aacaagaaga aattcctgca
    16261 ctgactgacg aacttgaaac tcactatgaa aaaagcggta tgacagcgaa tgaaatttca
    16321 ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca atcaattgca aatgaatatg
    16381 cagcagacag ccaaacttaa agcaatcgat ctccgccatg atccagtcaa agcagcgaaa
    16441 gctttattca aagaattggt gaaagaacca aaccgactgc atgaagcaag tcagtttctt
    16501 tatacgcatc tgccgaatct agttgatttg acaaataagt ataatgagat caatgaccat
    16561 gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa gcgcgcagat catcgaccag
    16621 ctgtcagctt taatcgcaca agattatcaa aaattcgttg ctgaggatct agatgatatc
    16681 gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg ataataaata tgaaccagaa
    16741 caaaaggatt aactcttttg ttcataaaag aagactcgga acattcagat gatgtctggc
    16801 tgtttcgagt actttttcta aagtagagac attcatgtaa gaatcgagaa cagattcaac
    16861 aaggaggaat tttttatgga tcataaaccg actgaaacaa aagatgttac accagttaac
    16921 gatacacttg aagatttatt gaacaacccg ttttcaactc caacagatac gctgaccact
    16981 tcacaacaag cggagattga tcagctccaa gagcaacaga cagctgctcg tttaattgac
    17041 aaactaccgg cggaacgcca agaacaagct aaacagctag cagcaaaaat cgatgctaat
    17101 gatgcacagt ctgtcatcag ctacggatcg gctgcccaag caaaattaag tgaattctca
    17161 caatcgatgt tgaatcatgt gcaagcacaa gatattggtc ctgtaggaga ttccttaact
    17221 gaactgatgt atcgtctcca agaagccaat cctgacgaac tgcgtgccgg agaagggaac
    17281 atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct atgaagtgac agcaaaatat
    17341 caaaagattg gtgcacaaat tgacaagatt tcagtgaaat tagataaaga aaaagacggt
    17401 ctgctaaaag ataatctgat gttagaacag ctttaccaga aaaacaaaga ctactttgat
    17461 gcactgaata tttatatcgc tgctggagaa ttgaaaatgg aagaacttca aacaacgatc
    17521 attccagaag cgatgaaaag agcagaagaa actggcgatc agatggatgt tcagattgcc
    17581 aatgattaca cgcaattcct agatcgtctg gataaacgta cgcatgatct tcgcttagct
    17641 agacagatca cgatccagca agcaccacaa atccgtttga tccaaaacac aaatcaggcc
    17701 ttggctgaaa agatccaagc atctatcgct acagccattc cgctgtggaa aaaccaagtc
    17761 gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa ctgctcaacg ccaagtctct
    17821 gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc tgaaaatctc agccatcgaa
    17881 acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga cccttcaaac cactcaaaat
    17941 gatttgatcg aaacaatcca agagacattg cgtatccaaa aagaaggaaa agaaaaacgt
    18001 cgtcatgcag aagtagagct tggacacatg gaagaagatc tgaaacaaaa actgttggat
    18061 ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa cgcccaacaa cagtttatta
    18121 caactgttga tgggcgtttt tcttatcttt cttctaactt ctccattgga gtctatttct
    18181 cattcatctt gctgtgtcca tagccatagc caaaataaat gatcagtcca ataaccatta
    18241 cgataccgaa cacgatccat gtcacagttt gcagacgaag catcaataca atacaagtca
    18301 agatggagac gattggcaat agcggaacga atggcacttt gaattctcca gcttttggtt
    18361 tgccaaacat ttttctcaac ttcagtaagc ctagactaac aagcatcaaa taggcgagcg
    18421 ccatgatatt cgttagttcc gctaatacat tcaatgggaa aaatccggca aagaatacag
    18481 ctgccacacc agcggcataa gtcgcttttt tcggtgtacg atgcttttga tcgatttctg
    18541 tcataaactt aggaagtaag ccatccttgc tgacagcaaa cagtaaacga gcaagtgaat
    18601 acatcatcga gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg atcaccgcgc
    18661 ccactgttcc atgtcctaca tacctcattg caaaagccac tgggtcattg actcctagtt
    18721 cagtatacgg aacgattcct gtcaagatca gcgtcacgat gatgtaaagg acagtagcaa
    18781 tcagtatcga accaatgatt cctcttggca catctttttg cgggtttttg acttcctcgg
    18841 ctgccattgc aacggcatca aatcccaaga aagcaaaaaa cacaagagct gcaccatcta
    18901 gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta acgaaaaata
    18961 ttcctacaat gataaacaac gcgatgatac caaatttgac atataccatg gcattattca
    19021 aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt gacgaatatc actacgagca
    19081 ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta cccgccagtt aatgcttgtg
    19141 gaagttcggc ccctaaactt ttcaaaaaac cttgcatata gccagaccat ccggaagcaa
    19201 cagaggaaac cgctagtaaa aattccccga tcaacaacca ccctgtcagc caggcagcaa
    19261 attcaccaaa tacaacatac agataggcat atggcccacc aatcactggc actcgcgaag
    19321 caaattctgc gaaacttagt ccagacaaaa tgacaacgat tgcagctaag acaaatgaaa
    19381 gtgacagtgc tggaccagca ttttgattgg ccgcaacccc tgtcaccaca aatattcccg
    19441 taccgatgat cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt tcttttttca
    19501 tctcactcgt atgattgaga ttgatctctt tcttacgaaa caaatccatt gaagttcctc
    19561 cccattttag gttggaaaac agcgattaaa ctgttttcat ataccctaaa aggataccac
    19621 ctaaaatgac taaaaggcaa ccaacaataa cataaatgag ctcttttttc gtttttcttt
    19681 cgccaaggat gaaaatccca cctaaagtag agataatgat ccccatttgt gaaagtgaga
    19741 agccaacagc taaaccaagt gatttgactg ttagaagcat gcagatgttt cctaagcccc
    19801 ataataatcc acagatcatg tttttccaca caaactgatc gactttaact ttacggaaag
    19861 caaagaagct tgcgcctaag atcattccga tactttgagg taagataatt gacatcgcat
    19921 ccaaattcgc ccagttgacg atgatggtat atacaccata accaatcgta gagaagatca
    19981 atgctctaaa acccttacca aaatcaagca ttttattatc tgtatctatt tttccttcgt
    20041 catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca ataataccgt
    20101 atacataatc tttcgtttgt gtccattcat ggaagaaaac ggctcccgca atcgtattca
    20161 aaattaattg aaaaccagta gacaatggta gccctacaga aacacccatg tacttcattc
    20221 catggaactg tccattttgt ccaacactcc aagctaagcc ggataaaaat ccgatcaaga
    20281 aaatccaagg ggtgattaca ggactaacaa cgaaaaagac aactaaagaa aacagaaatg
    20341 caccgatcgt cattcctagt gtttgttgat tggcatcgcc accaatttta ccacttacta
    20401 aaccaatact accccatgcc aacataggga ctaatgctat taaaatttcc ataaccaatc
    20461 tccttttatt tcacatgata agaaacttat cagcgaaaac gttaacaaca caagcacttt
    20521 ttttcgtttc taacgatttt ctaacgtttt tcattataaa aatttaagat tgggaaaaat
    20581 aaagtcattg ttgtcccttt tccttcagtt gattcagccg tcaaatggat acctaatttt
    20641 tcggccagat tttttgctaa ataaagtcct aatcctgttg aatgtgtttt gctaagtcga
    20701 ccatttctcc cagtaaatcc cttatcaaag atccgtttca agtcttccgc tgggataccg
    20761 atccctgaat ctttaactga aaggctgact ccttgcgctt ccttttctat gctcacaatg
    20821 atgtttccgt gatccggcgt atatttgatg gcattactaa gcaattggtt gaagataaaa
    20881 gcgatccatt tagcatcggt caaaacagta tgatcctcac caacgatctc gtagcgaata
    20941 tttttttgga taaaataatt tgcctgactg cgtatcacag gctgaatgat tccttttagt
    21001 ccatattctt ggatgaggta gtcccgcgag aaactgtcga gtcttgcata atacaaaact
    21061 tgttctacgt attcatcgat ttttgataat tcattttcta gtaatgtata cttttgatcg
    21121 tcaatatcga actcgatcga acgaactagc aatttagaag cggaaagtgg cactttgatt
    21181 tcatggaccc atgagtcaat ataatctttt tggtcttgtt gattactgat tgctgattgc
    21241 attacttctt ggtgttcacg gatcagctga ttgatatatt cttctaccag tttttcttct
    21301 tctgtcctcc caccactcaa ataattttgc aaaggggaag ttttttgtat gtcggctaat
    21361 tttccccacc atcttctctt gctcaagtaa ccacccagca gataaaaaat cagaaagacg
    21421 ccctcaatca aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc
    21481 cacatcacaa agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa tgtccactga
    21541 tctttcaaat atttaaaaaa attcatctta accgctcatc ctttcaggga acgatatatc
    21601 cctgtcctac ttttgtttcg atatatcctt ggatacctgc ctgttcgatt tttcggcgta
    21661 accgattgat gttgactgtc aatgtattgt catctacaaa tcgttcgtca tcccacagtg
    21721 ctcgcagcaa tttttcccga cttaagatct ttccattgtg cttcatcaaa ataaataata
    21781 gacgatattc atttttgctc aaatcaatga tctctccgtt gatttccata cttccgttgt
    21841 caacattcaa cgtaatgcca ttgtggctca tcatttcact agataattct gtgtagttgt
    21901 aagaacggcg aagtaatgca ttgatttttg caatcagtac atcgatttgg aaatgttttg
    21961 tcacaaaatc atctgccccc atgttcatag ccatgatcat atccatattc gtattcctgc
    22021 tggagataaa aatgatcggt actttcgata cttcacgtat tttttgacac caataatagc
    22081 catccaaaac gggaagatta atatctagta atacaagctg tggttcttct ctttcaaagt
    22141 ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc aaattgccat ttctcaagtt
    22201 cctccatgat caattgtcga atgatctctt cgtcttcgac taccatgatt tttgccataa
    22261 aaaatccctc catctttctt cttgctacta caatatcaaa gaagcccttg tgaaacaagt
    22321 tggcttacct ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa tatgcgattt
    22381 aatcgcgttt gtctcttcca agcagcttaa atttattatg ttattcttaa taattggtct
    22441 attatctgtt atttttcttt cgttgttttt gtcttttaaa ttttcgataa caccaataac
    22501 cgaaaagcgt tagcactagc aatataccta gaaggagtat aagtaatcga taaagatggt
    22561 atttttttgt ctgttctatc tttgaagaga cttcttctgt aaagggaact cgtcttcctg
    22621 tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt gtgactagat
    22681 cctgcttttc tctaattgct aatgtatttg tttttgttgg aaggacaaca attttctcaa
    22741 cgacttcata agcgagcatc cgaccataca cttcaagata gaatcggtca tcttgctcca
    22801 tttcttcaag atccgtaaag agttttttct cagcaatacc gctatgggcg gtaatcacgg
    22861 aatgtgtact ctctcctcca gttggaaaag aggtgccttg caggactgtc gctcctctat
    22921 ctaacaaaag atcattcgtt tcatcaaata aaggcaagct aacattgatt ttcggaatat
    22981 agatggctcc gatcatatgt tgttcgtaat attcttttcc tggattttgg acatttctaa
    23041 ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg gattactgat gtatgagcaa
    23101 gcgtctgatt tttcttttca tgcactgcta attgttgctc atgtttttct tctttatcgc
    23161 gttcaagctg cttttgataa ttctcgatcc ttctttgatc caaataattg ctgagagcat
    23221 ctgctaaaaa tggatatgta aaaactgcag ctccggcaag aaacatcact gccataaata
    23281 attgtaataa aagactgatt tgggtttgag gtctattacc tattttcttt ttcattttct
    23341 tcaccttctt atttaaaaaa actgcagcaa cctttataaa accgattgct gcagcttatt
    23401 tttgttgctt atacttcaga cttcatttta gatttacggt accagctgta cgctccaatc
    23461 atcaaactta ttccgattag taagaatgcc aagagcccgt tcccaccagt agcaggaagt
    23521 gacccgcgtt tcatattcgg tactctttct ggccctggat tttctactcc tacgatctga
    23581 atttcttcat tatagcttcc ataagagatg ataaattcag taaaggctgc ctcacctgtt
    23641 ggaagtacat aaccttctgg cgccatggtc tctttcattt gataggttcc ataatctaac
    23701 ccttgaacct caaagtatcc atttccatct gcgacaaatt ctgttgcttg tccgcttgtt
    23761 gcccactcaa ttctatctgc tcctgattca taaacagcta aaggcgactc ccctttataa
    23821 aatattgcgt attcagaacg gtcttcattc cacaattcga atcttgcatc tttaagcaat
    23881 tcactgcttg atgcatcata tttttcaaac tttcttccac ctgttgtgac tttctctggc
    23941 ggctctactc cttcttcata atcaaattca tcatttcgtc cgatttcaat aaccgctgtg
    24001 ttgttatgga aatcatccgc ttctagttct gtggaaacct tcaaattata cgtgatcgtt
    24061 aaatctcctc cacgtgcatc tgctaatcta cctaaagtat ctactttggc tactccattt
    24121 tcttctgtca aaatcgtcac gacaaacccg ttgcttacta cttctactat ataatcttgt
    24181 ccttttgtta aaatcgtctc gccggctcgc acttctattt cctgatcata ataagttaga
    24241 ccaactgtag gcgtatctgt gattttgtaa tttttgataa cagcgcttcc gttttcaagg
    24301 acagaatcga tgaaataagg aataggtgct gtgatcgtgt aagaagccgt ttttcctctt
    24361 tctagatcaa tataagaaat cgtttcttgt tctccagctt cattaataat cgttacttgt
    24421 ctaaggtcgc tttcctctac attcatctgt ttatctgctt ctctgatttc gtttttcgga
    24481 tacaaatgca catctgtatt gtatgtgtct ttccatgtag aaccatccca cacgccatcc
    24541 accatatcag gcatcatcac tggcatcata acaaccattg gagacgcgat tctggaaata
    24601 tgagctggcg aataagtttc taagaaaaga tagactttat ctctagaaga aggtgcttct
    24661 tccgtcactt ggacggtaga gaataccgct tctccagctt cattcgttgt ttgtttgtca
    24721 atacttgtca gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg aatccattca
    24781 atcgcttcgg cagaattaag accgtcttct cgttccggtg ttaaaggatt atctcgaatc
    24841 aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat cgactcctgg aatattttct
    24901 tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt cttccggaac agatgagaaa
    24961 ccttttttgt gtaatgtaat cgtcacttca cttggttttt gtgcgacttc ttcggctttg
    25021 actagttttc cgttcccgaa aatccctatt cccgcagtaa cgagtaacag tacactcatt
    25081 cccgtgacaa tcattttttt attcattttt ttctccccgt tcttttttta ttaataaaac
    25141 agcacctaac aataaaagaa ttcctagtaa agaaatcaac gttcttgctt cattcgtttg
    25201 cgggaatacg gaccattttc ctgattgacc tggattgctt ggaccactcg gctctttcgg
    25261 tttgtccgtt gtttctttta cggtctcgtt ataaacacgt ggtgttgcgg attcataggc
    25321 agagactgga actttcccgg attctaattc agccatctcc tgtccgttaa ctactacata
    25381 ttgtggttgg ccttttgcat ccacccaaaa ttcagggatg atgacctcga ttctcttact
    25441 tgcttcatcc atctcgtaac cttccacacc ttttaattct tcaaaataat aggtgccaga
    25501 aggcaagaaa cgttgtcctg tcgtaacaag gccctcttta tccgaaatga atttgcttac
    25561 atttttgtca ttcaaaggat cattgtctgc tggtttgatc cattgatttt tcaagtcatt
    25621 agcaggagcc atatctaaat aatatttccc cccttctatc atctgatata gagcaaaaac
    25681 agcaccttct aatggttttc ctttttcagt tgttcctctt tgacgtccat acttgaagaa
    25741 gtatggatct cttagataac cgacattttt aggatagata tgaatctctt gcagttcctc
    25801 ggttgattct gtcgggtgat aaattggcaa aatagcagca attgggatag ctgtctgatc
    25861 gatatcaaca tccaatccaa cttctggatc aatcgattct tcgaaaatca aataaactgc
    25921 atcccgatct tggtttttct taggtgcttt gatacgtgca atcccgtctc ctaatccgcc
    25981 aaccgtatct ttcccagttg ttaccgaagt ccctatttgg acaagccttt cttctgtgat
    26041 tagctgacgg atattgtttc tgttcgtttc agcgatttgc tggacgaatt ctttaggagt
    26101 catgttttca gcagcttttt tttcatgata ataatcggtc atgtcgtaaa tggcaaatgt
    26161 cgctccattt agtggcaccg tctgtgaaat gatatctgct gtttcccctg cttcctcaga
    26221 gatagccaag ccatcatttt catggtattc aaattgatcg ttataatcga tatctcgaat
    26281 catccgttta tgcaacacga attccacgac atcttcccct gattcttctg ccgacatcgc
    26341 atgtggaaga aggactgctg caagcggtag taaaacaagt aattgaagca tcaaacacaa
    26401 cagctttttc attaccttgc tccctccttt ttagattgtc tgacaaacaa accataggct
    26461 gctcctgcaa gcgccatgaa gaatgcagca agcaggatat agaataatcg tccggatcct
    26521 cctgtagcag gcaacggatt ttcaggatcg ttcccgacac taagctgtaa tctgattcga
    26581 tttgtctcca aaagttcata ttctaactct tgctgtagat caggcgtctc tgcttcttcg
    26641 tttggataat acgaaatctc aacttttagt gtacccgctt cttttcctgg aactacccga
    26701 tcattttcta tgtcaggttc ttctcgataa ctaatttcta aaataaagta acctggaagt
    26761 tttctatagc tgtcaggtga cttcacttcc ttgatccgat agaccccttc caacaagtct
    26821 gaagcattga atagggaaga atcgtttgga tctggtgcca agcctccctc gatgatttca
    26881 tactctcctg cactattttg tttctcaatt gaaaattctg cgccttcaag acgtcggcta
    26941 ttcatactgt caattttcaa aagttccaaa tcgattggcg ctagttcatc ttctaaggtt
    27001 acttgtagta caccatcgat gatttcatag ccttccggca agggagcagt ttcgtcttcg
    27061 atgaccgtat cttcaatgat aaacttacct tcttcattca gtgcaaactg gaacacttga
    27121 tcagatagcc gatacccatt tggtgcgaca gattccgaga cctcatattc accaatagac
    27181 agatgcagag gttggtcgta atttaaatcg acatactttt ctactcctac ggcacgagaa
    27241 tagaagtatc cgatcccagg caaacgtttc gattcttctg gttctaacgg attaccagtg
    27301 tatgctcgat acagcgggaa ggaagaatcc cccactttcc tgatatcgaa acctgcacca
    27361 tctaatgtat ggccttcatg gtctaatttt ctaactcgga tctcccgata atcaaacgta
    27421 ttgtaagcag tgaatgctac ccataaacga tcctcttcat ctgttgtata cgtgagatat
    27481 tgccaatttt cttcacctgt ttgcttgagt tggatagtcc ctatagcatt atcgtaaagt
    27541 cgcaagatcc atgtgtcatt ttcatcaggt tctttgaaac cttctgggtt ctggctctca
    27601 atgattttgt attctgcata gttggtagtt tcttcactat ccttataaac aaatgaatct
    27661 ggctctccga ttggatgaat aactttctca ccatccacga ttacatattt cccgtcttca
    27721 tctactgaat agaacgtgag tctccctttc tcttcctcta cttcgttcac accagaaagc
    27781 cctttcgcta ctggatatcc gatttccagt ccgttaccat ctgtttcata aagttcaaac
    27841 tctgcgccat tgattggcat attactgcct tcaattagtt tattgatatt catgtggatt
    27901 ggcttgaatt catttcgcat ttccagtttg acatacaact catcatactc tgtgccttct
    27961 atgttaggct cttgtacaac agaaagcttg tgatattctc cttcataata ggaattcgga
    28021 tctttacctt ccggtccgcc gactctcgaa ccagatgtat attcatctac aagctcgtag
    28081 tgacctaaca tctcatcttc gttggctgca tattttttga tttggaattg atacgtctca
    28141 tctactaact gataacgtgg tggtccagcg atctctctga tccaaaatat gtcgctgact
    28201 gctccagttt catctcctaa aatcggcatc ttcgtttcaa agatcgctat tccgtccgtg
    28261 gtagttttac ttgtttcgat tggttcggct tccccactct ctggattttg gtcatataat
    28321 gcaaattcga ctccagccaa cggctcgcca ttttgaccct tcttgctgac ttggaaatta
    28381 cggcctttat gttcgtaacg aatggaagaa gatgggaaat acatccgtcc agtagtgata
    28441 tcattctcat aatcctccag gttctgaata taggccatat tgttcactgt gcaaaaattt
    28501 ccgtcttgag ctcccgcatc aagttttact tggtacttga tcctgaccat ttccccttca
    28561 cctaaagaaa tcggagaaaa tttataagct tggttttccc gtccgacata cgttgtcgga
    28621 gtcgccacta cttctggtgg ggcttgtcca tgacgttctt catcccatat ttctggctct
    28681 tggtacttca tgccgttttc tcctggatct agatggtaaa actgaatatc tgccgatctg
    28741 ccagctccaa atggtgagac attaaaacca gtttcatcat aaagtgtggc ttgctggatc
    28801 gttctcttga aggtagaaga gatatcctcc aaggctgtaa caatgtcatt cttgttgttc
    28861 gcattgtaat atcgcggtgt tacgccatct tcttctaaat cagcaatatt tttcaataca
    28921 tttcgtccaa tagcatctag tcgttgtctc ccgctaacgt ttccatcgat tcctaaacca
    28981 atggagaaga tacttggcgg gttctcttga tacttgtttt tcagccaatg atgataagca
    29041 actgtgttga tcgcagatga cttttcttgc gaagtaggac tgccattcgt attgttatta
    29101 gaagaaccat caccaaccca gtgagtcgct ttattatctc cttcttttac ctctccataa
    29161 cgatagtgta catttacttc atcttctgga cggttaaagt cagatgcata ggtaactgga
    29221 tgagtaaacc ctgcattaga agtattccca gaaaactcct caaaatttct gaacaaaacg
    29281 tcattatctt cagcaatctt atcttccatc acagtccagt tgctccaagc tccattagtg
    29341 ctcgtacgat aacttgactg aatcggtgca taactgaagg tcggtgtccc atctcccaca
    29401 acgattagga ttttttctgg atttctattt tctccgccat tgtctttata caatgtttca
    29461 tagccatttt taagccctag tgtaagcgga gttcctccga ttggtgtacg atttaatgct
    29521 gtattctcac ttcccgtcaa acgattgata tcatttgtca atggaaagat gtccgaactg
    29581 atttgctcat ggttattgat agctgtacta ttatggtaaa aattcaccat accgattcga
    29641 atatccagac tcgggtcaga gagcagatct ttactcattt cgtttactgc ctcaatcaac
    29701 gcagcatttt tagtttgact attggtccca gcggttaatt cactcatact cgctgactta
    29761 tctagaacaa aaacgatatc gattggactg atttcctttt tctcgccccc gatgacatct
    29821 agatagacat tgtaaagggt tgttgcatct tcaggatgta gatcagatgc tgtgctgctt
    29881 ggcagaacag gttctaccca ttttttgatg tacgccccgt tataatccaa gtaactgttt
    29941 tcaaaattcc gttcatttcc ccaaagctga acggtttgag ctggaacgtt tctatcttct
    30001 tcgcctgcag ttgatccatt attagtagca tagtcgtaat ttcgccagtt cgctcccccg
    30061 tttgtataat cactatcatc gatcaagttc tctccgtcat atggcgagcc tttgtctcct
    30121 tgatagcgtt cattgctttg tgtccaattt cctaacactt ttgtatagtc attcgttgta
    30181 aacttcgggt agcgatgctg ttcgttttca acttctccgc taggatagat atattcatag
    30241 gggtcttgcg tagctgttgg cccaagacgc tccaaaatac cgttgaataa ctggcgttga
    30301 atgcgctgga gtgcttgacc gctgggtacg attggttcgt ccagcttttc catctcattt
    30361 tcttcgctac gttgaatttc agcggaatct tctgtatcag tttcattttc ttgtaattct
    30421 tggttatcta ttctttcatc aaccaccact tgaagaggta gttacccttc gttcacatcg
    30481 gggatctttt ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga ataaactttt
    30541 ccgacatacc attcttgacc atcgatcgtc tcgactttta ggtcttctcc atcttcatca
    30601 taggaggata gacctgttcc acgaacgttt tttacggtac caatcccggc ggctgccgca
    30661 tcgattgcta aacgtattct accttcattg gcactttcct ttttttcaaa agccagatgc
    30721 cattcaaggg tttcttcttt ttcaatatac ttcccttcaa tttttgcata gtcctcatct
    30781 aagaatacct catcctcctt tgtttctgtt gactcagcag ctacatccag ggtcgtcata
    30841 tgatagactg actggaaata tggtccaaat agcaaactga cagttactaa ctgcaaaagc
    30901 ttcaatttta accctttcac atccttatac tcctttcaaa aatatttgtc ttctttcaca
    30961 acaatcaaca aaacaatgcc catctaatta taatttaatt taattttaat tttaaatcaa
    31021 atgatatcct ttttagttaa a
//
LOCUS       DAZKOW010000022        30167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000022 DAZKOW010000000
VERSION     DAZKOW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.022"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(159..1469)
                     /locus_tag="ACG912_001020"
     CDS             complement(159..1469)
                     /locus_tag="ACG912_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFQ0512357.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2839)
                     /locus_tag="ACG912_001021"
     CDS             complement(1481..2839)
                     /locus_tag="ACG912_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0512358.1"
                     /translation="MSDKIGYMFGDLGNCFILGLVNSFLMIYYTNVLGVSGAIVGTLF
                     LISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKYPFCLVTVILFLPFVQHLPMT
                     VKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDRTSLSTFRSIGSAIGGSTTGF
                     FIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYMLTCKLTTERVRVVKNEKVSA
                     SQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLFNDYFHNKQAMSVALLFTYGT
                     VVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFLHITNAWVYLILLFIATLGSG
                     LFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARKVGQACAGAVGGFMLALIGYQ
                     SSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLVGYPLNRSKTVFIGEELKKIN
                     ER"
     gene            complement(3041..4609)
                     /locus_tag="ACG912_001022"
     CDS             complement(3041..4609)
                     /locus_tag="ACG912_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFQ0512359.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..5023)
                     /locus_tag="ACG912_001023"
     CDS             complement(4613..5023)
                     /locus_tag="ACG912_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0512360.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5020..5943)
                     /locus_tag="ACG912_001024"
     CDS             complement(5020..5943)
                     /locus_tag="ACG912_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFQ0512361.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5940..6818)
                     /locus_tag="ACG912_001025"
     CDS             complement(5940..6818)
                     /locus_tag="ACG912_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFQ0512362.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6805..8142)
                     /locus_tag="ACG912_001026"
     CDS             complement(6805..8142)
                     /locus_tag="ACG912_001026"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFQ0512363.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8145..9248)
                     /locus_tag="ACG912_001027"
     CDS             complement(8145..9248)
                     /locus_tag="ACG912_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0512364.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9322..10830)
                     /locus_tag="ACG912_001028"
     CDS             complement(9322..10830)
                     /locus_tag="ACG912_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0512365.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10796..11488)
                     /locus_tag="ACG912_001029"
     CDS             complement(10796..11488)
                     /locus_tag="ACG912_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFQ0512366.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11510..12484)
                     /locus_tag="ACG912_001030"
     CDS             complement(11510..12484)
                     /locus_tag="ACG912_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0512367.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12784..13656
                     /locus_tag="ACG912_001031"
     CDS             12784..13656
                     /locus_tag="ACG912_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0512368.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14114..16312)
                     /locus_tag="ACG912_001032"
     CDS             complement(14114..16312)
                     /locus_tag="ACG912_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512369.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16544..17536
                     /locus_tag="ACG912_001033"
     CDS             16544..17536
                     /locus_tag="ACG912_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0512370.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17596..19017
                     /locus_tag="ACG912_001034"
     CDS             17596..19017
                     /locus_tag="ACG912_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFQ0512371.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19010..20509
                     /locus_tag="ACG912_001035"
     CDS             19010..20509
                     /locus_tag="ACG912_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFQ0512372.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20548..21942
                     /gene="uxaC"
                     /locus_tag="ACG912_001036"
     CDS             20548..21942
                     /gene="uxaC"
                     /locus_tag="ACG912_001036"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0512373.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22101..22784
                     /locus_tag="ACG912_001037"
     CDS             22101..22784
                     /locus_tag="ACG912_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFQ0512374.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22786..23622
                     /locus_tag="ACG912_001038"
     CDS             22786..23622
                     /locus_tag="ACG912_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0512375.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23633..25387
                     /locus_tag="ACG912_001039"
     CDS             23633..25387
                     /locus_tag="ACG912_001039"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512376.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25663..26004)
                     /locus_tag="ACG912_001040"
     CDS             complement(25663..26004)
                     /locus_tag="ACG912_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0512377.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26072..28243)
                     /locus_tag="ACG912_001041"
     CDS             complement(26072..28243)
                     /locus_tag="ACG912_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0512378.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28454..29311
                     /locus_tag="ACG912_001042"
     CDS             28454..29311
                     /locus_tag="ACG912_001042"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFQ0512379.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29382..>30167)
                     /locus_tag="ACG912_001043"
     CDS             complement(29382..>30167)
                     /locus_tag="ACG912_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0512380.1"
                     /translation="KLSIPTTTPEETLDQVFEFFDRYELKGIGIGSFGPIGIDKTKDN
                     YGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTDVNAAAYGELKQGAAQGKNSCIYLT
                     VGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPDDHYEGTCPYHKDCLEGLAAGPSIE
                     ARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLTLAPEKIILGGGVMNQDHLLQKIRQ
                     QFVELMGGYMETPQVSEYIVRWGLPNESGIIGSLLLAEHEHQK"
BASE COUNT         9028 a         6223 c         5346 g         9570 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgcgaaataa aaaaatgcaa
     4681 aaggaaacgc aataaactga gtgccaatca acaatatgaa tcctgttata aaatacaaac
     4741 cagtggcagt tgttaagtat tttcctttag cagtcaaaaa caatgctact gctaaaaaag
     4801 caagtgaaat aaacaacaca acggcaaaca gaccagtcat gtcctgtaat aatccaaatg
     4861 tattttctaa agacaaagtt gaatgatcta cagcaagcga ctgataactt tttttaaact
     4921 ctgacgcaga catattactt aaagtaactg ctaacccgcc aaagaaaatc aaagatagac
     4981 tgccaccgac cattcctagc aatagttccc attttctttt catttctgaa ccccctgctt
     5041 ttctaaatgt atccattcag gtcttttgat cgatgtttca taattgttat tacgttcaga
     5101 gaatttgaca gtctgttcat ttttcttgtc atcccaatta tcccaaccag ttgggtgaac
     5161 atgcccacct atcttacacg cttcaaataa gacatttgca tacggacgcc acggtcttcc
     5221 taaatagacg tttctagtat caggttctgc agtcaaggta cagctttcaa aacgataccc
     5281 tgttttttga tttttcggcg tagatgctgc tgtgatgtat ccaggatttt tactggctaa
     5341 gctatggata tgacactgat gaaaaacggc atctgcccca ccaaatatga aatccaccgt
     5401 tccttcaatc cagcaatatt cataggtctg acgacaatac tcaggattgg cagaagccgg
     5461 tgtacaaaaa ggagtaccat ctttttgcaa atcaggcaac ggacccgtgc ataaggtatc
     5521 ctgataacca gatagtcgac agttttttaa agatacgcga tctcctgaat tgaataaagc
     5581 aacagcctgt cctacctttt ctcctggacc tgctgtattt tcgatgtgaa gattttctaa
     5641 tacgacattc gatccttcta agaaaaaagt agcacttcgg aaagtcccta actggctacc
     5701 gtcaagatac tcctgttttg caaaacgatt accggagata actactgttc ctgctcccac
     5761 aagtttaaaa ttggataatc tgctttctat atattcatca tatcttcctt cttttactat
     5821 caagaccttt ggatggctat ccggcaggcc tgccaaataa tcgattcctt cttggatagt
     5881 ttcaaaatcc gtcggggtat ctatagcatt ctttccaatt atcagtttgt ctttcatcct
     5941 cataaatgct cccttatgaa ttgtttccaa atatttctca tttcttgtgt ttccatatga
     6001 ccgcctgtta aagaaaaaga agaaaagttt tctggactac ccattgattg atactttttg
     6061 tccagccctt ttctcagctt cagtacgcct tcgtccaagc acatcgtatc gttttttcca
     6121 actaagctaa gacgagggcg cggagcgatt ttttcttgga ttgctaacgt tgtataagct
     6181 gtcaatagat ttggcacata gtaataaaac ccatgatgat ctaggcggcg atgtagcaaa
     6241 agtatttcga tgtccacctg tgccgctaga tcaatacaaa ctttgacacg agaatccaat
     6301 gctgtcaccc accagcttaa taagcctccc atagaaagac caatcgttgc gagccgttga
     6361 gtatctgtgt cttcccttgt ctccaaataa tcgatcaagc tgatcacatc ataaatacgc
     6421 attccccata acgtttttcc tgttaataaa aattgtttga acaactcgct ttcagaaata
     6481 cctccgcgtt cttcaaatcc ccaagcatca atcgaccaaa cagcatatcc cattcctgtc
     6541 agtacttctg caaaagatgg agaagctaag taaggagcac catggatcaa ttcacttttc
     6601 ccgactgaaa aatctccgcc gtgagaatgc aggtaaagga ccggcggaaa cggtccttct
     6661 ttttctggat aagcatagag ggctggtact gtttccaatt catttaagta caattgatag
     6721 tattcaagca taaaaccgtt ttgtttttct cggtataatc gttttccact aggggtatgt
     6781 ctgctagatg gagcacctaa aaaatcattc aactgcataa gcaaagtttt tacgaacaac
     6841 cagttcatcc atattttctg attgatttga ttggaatttc tcaatactga tttctttagt
     6901 attttctaat agaaatgctg gtccctcgtg attttcaatc gacatctggt taaaggaaac
     6961 cttacccgca aagccaatat aaaatccttg tctgctcatt tcctctattc ctgtcatcat
     7021 agcaggaaca ccgggaactg catcttgggc catcgagaca tcgatattgt caaatgtgat
     7081 ttcttgtatg aattgttccg ctaatccata aataaaacct gcagaagcat ggacattcct
     7141 tgccgtgata ttcgaaaaat ggatccttct gaatgctggt gtccgttcat ctatcggata
     7201 agcctttttt tcccatacat acggttcttt ccctcgcgga ccacagaaat aataaagatt
     7261 cagtataaat ggacacatca cattatccat gactatattg gatactcgaa tatcttcaac
     7321 gatcccgcct cgacctcgtc tagatttcaa tcgtattcct cgatcagttt cttggaagat
     7381 acaatttgaa atggtaatat tacgaatact accactcatt tcacttccta atacgacacc
     7441 gccatgtcca tggaccatcg tgcaatttgt gatcgtgata ttttcgcaag cgatcctttc
     7501 gtacgtatct tcagtccctg ccttgattgc aatacagtca tctcccacgt caatatggca
     7561 attactaata cgcacatttt tgcatgattc tggatcaatt ccgtctgtat ttggtgaatc
     7621 cgctgggttc aagatcgtca agttatcaaa cgttgcatta ctgcaaagaa tcgggttgat
     7681 cgtccaactt ggtgactgga tcaatttaat atctttcaca gtaattcgat gacagttatg
     7741 gaaactcatc aatttaggac gaggataagc gagattatct ggttcatttc taaaagtatg
     7801 ccaccatttt tttccatttc catctaatgt accgaaacca gtgacagaga tgttctctac
     7861 attttgagca tagatacagg aagcataaac ttttcgatgg actccttccc aacgtgaatg
     7921 aacaacagga taatcttttg gatcgtcaga gaatttcaga accgctcctg cgcttaaatg
     7981 aagttcgaca ttgcttttta aaaataaagc tcctgttaga aattcacctg ctggaacaac
     8041 tacggtcccg cctccgtcac ttgctgcagc atcaatggct tgttgtattg cttcagtatt
     8101 caattcatca attgatgcgc caaatgtcaa aatatcatac atatttataa tgcctccact
     8161 tcaatcaatg cttgcaagaa tgcaccaacg cctttttgat cattacaaat aattggttca
     8221 ctgatgtagt aagcatatga accatctcgc tgatctgctc cgcctaaccc tgctacttga
     8281 cagtttttat tcaagttcag ccagccttct tttgtttcca gaacaaattc atcaagtaat
     8341 ccttgatgtg atttcttcac ttgtggaagc cattcttctt tgttcagcag tcccaaacgt
     8401 attcctttag ccattgcgta aacaaacata ctactgccgg aagcttccag ataatttcct
     8461 ttccgttttg tttctgttgt tacttgatac catgtcccgc tgttttcttc ttgatatgga
     8521 agtaatgcag ccagtgtctt ttctagaatt tctttcagca aagaacaatc tgtttcttca
     8581 tgaagtaatt cataggtatc tgctgcagcc atcaaatacc agccaatcga tcggctccaa
     8641 aaatgatggg atagacctgt ttttggatca gcccatggtt ggaccttctt ttcatcccat
     8701 gcatgaatca gcaaatttgt ttttggatcg aatagatgtt tataactgat ttcaaactgt
     8761 ctcaaaacat cttggtaatc tttttcttca ctaaaattct ggataaattc agcataaaaa
     8821 ggagaaccca tatataagcc atccaaccaa atttgataag gataaatttc tttatgccaa
     8881 aaagctcctt cggatgtgcg tggatggttt gccaactgct gggaaaggag ttctgctgct
     8941 tttttatatt tttcttcgcc tgtttctttg tacaataaaa agaaaagctt tcctgtattt
     9001 acatggtcga tattgtattc ttcaacgctg tagccacgga tcgttccatc ttcttgcacg
     9061 aaatagtcga tattgtcttt tatgaaatca aaataaattt tttcttgggt tcgttcccaa
     9121 agtaattgga accctttaag tacgacgcca tagtcataag accattttcc tttataccac
     9181 ttttcttcat ataagcgagg tgttctttcc atgatacttt ttgctaattt ctttgaccag
     9241 ttttccatta tttgctctcc tttaaaaatt aaaataggat ttgggacaaa taccaagaag
     9301 tatttgtccc aatcgtctat attactgtgc cgctgctttg tatgcttcct cgtactcttg
     9361 tgtgattcga tcgcctcctt gtgctttcca gtcttctaca gcttttttga atccatcttc
     9421 gtcaagctgt cccatgatgt attgcaccgt agcatcaatg acgatttttt caatttctga
     9481 tcctacttca ttgttcgtcg gtgaatccaa tggcacagaa ggatccatta cagcaaattc
     9541 ttcattttct ttgatcaatt gattagagaa ttctttttca gggtctgtat ctttgaaaga
     9601 gaacgtcact tcactaggac gagagcttga taacggttgc acctcttgtt gccaaagatc
     9661 catatcgata aattcgatag aaccatcatc attcaattca taatgcgttc cttcgatacc
     9721 gccagtcatc aatttataca catcttcttc taaaagatca tcaacaaatt gaagtaattt
     9781 cttcaattgt gcttcgtctt ttacttgaga tttgggaaat gctagcaagc cgccaatacc
     9841 atttccttct gaccaaacat gataaccgcc gccttcacca tctgagatct tgtttacagg
     9901 cactaattcc atatcatctt gaataccttg acccatcgtc ttcagattct tgatatccac
     9961 cattccagtg tagattccag ttcttccttg tgcaaattgt tcttgctgat cagtcttttg
    10021 ggtaactgca aagtcttgtg ccaaatagcc attctcaaat agatctcttg aaaaattcaa
    10081 agctttcata tactcttctg tatcaaattc tggagtgaat ttgccattat cgtctacagc
    10141 ccagccattt ggtgctccaa aataagaagt gaaaagttta aagctggtat aacgaatatc
    10201 actaggcgca cgatcgccaa aaccaactgt gtcgtccaca ccatttccat caggatcgtt
    10261 ttctgtaaat tgtttagcta cttcataaag ctcatccatg gtagttggaa catctagtcc
    10321 tagattatcc aaccagtctt ttcgaatgac taagccagca cgagcgtagt ctttttgtat
    10381 cggtacgccg tacaaggcac cgtcgatact cgcagcttcg attctatctt cagaaatttg
    10441 acttaaattt ttatagtctt ttaaatattc tgatacatcc cagaatacgc ctgattttaa
    10501 cgcattgcgg acagaagagt ttttcaacat cgcaatttga agtgtaacga tatctcctag
    10561 ttcatcagaa gccaatgcag ttgttaatct ttcttctttt gaagcatcag gaatccagtt
    10621 gaatgtcagt tccgtatcgg ttttttcttc gatcaagttc acaatcgtgt ctgtaggcgg
    10681 tgaagcatga tgcaaaatat ttaaccaagt gattttagct tttccatctt ctgtctctga
    10741 agaactgtta cttgaactgc tgccgcatgc ggcaagtgct agaattcctg caaaactaag
    10801 acctaatttt acgagcgttg cttttttcat aaattttctc cttcactgtt ttaataatat
    10861 ttttgttttc ttctgttttt tctgtatatg agttcttcca ttccaaatgg caaaatatag
    10921 aatttgctgt ccacgtgact gcggaaaaga agagactgcc accgataaag gttaaaacac
    10981 ctggcattgt ttggatgatc atcagataaa tccctaccat acttaatatc aacactaatg
    11041 agtaatgaag atgtgagaat ccgaataaaa atgccatttt cattttgaaa aaatatcctt
    11101 tccaatcata atgagccatg attgggaaaa tatagagtgc tgagagcaca tatatgaacg
    11161 cagcaatata caaagcgatc ctaagatacc aattgtgtat gaaagaaaga tccacaagta
    11221 aaacaatccc gatggcgcca tatatccagc tcatcagcaa ggtttcacga tagttttctt
    11281 taaaatatgt gaagtacgtt ttcgtcaaag gaaacgtttc ttttgagcgc agccattttc
    11341 taatcactgc aaccatcgca tacgatgcag gaccgaaagt aaaaactccc aaacctgcga
    11401 tgactaaacc gccccacaga aaattcaaat aaaccaactg taaaatacga tttagccttc
    11461 cgtttatttt atctaatgac tgcagcatat ctttccctct ctttctatct tagtaaacgc
    11521 cgtcttctcc tagctttttg gcaatcttat tagctaaaat taccataaac aatcctacga
    11581 cccctttaaa caatcccatt gctgtactga aacttaactg tccattcttc aatccagctg
    11641 tataaatata ggtgtcgaaa atttctgcaa cgcttcgatt caatgaattc aataacaagt
    11701 acatatgctc gaaccctaag tctaatgtct gaccgatttt tagaatcagc aaggtaatga
    11761 ttactggacg aattgccggc aatgtaacat gccatgtttt gcgtaagcgt cctgctccat
    11821 ccatttctgc agcttcatac aactgtgtat cgactgcagt tatcgctgaa aggtagatga
    11881 tcgtagacca gcccaactct ttccagatca cttgtccgat atatactgtg cgtgtccatt
    11941 caggggatgt tagaaaactg attttttcaa atcccattgt tgcaaggaat tcattcaata
    12001 cccctccgtc aacattcaaa aatacataag taatcgaaac aatgattacc caagacatga
    12061 agtgagggat gtaaatgatc gtttgaatcc cgtttttcaa tcgtttgttc gttacttcat
    12121 ttaatagtaa agacaaaatg attggcatcg gaaaaaacac gaagatattt aatccgaaca
    12181 agatcaacgt atttcttaat aatgtaaaga atgtcggttc tgtaaacaaa cgaatgaaat
    12241 gtttgaaacc gacccaagga cttcccatta ttcctaaaaa tggttgataa tcttgaaaag
    12301 caataacgag tccccccata ggaagatacc taaaaatcaa gaaataaagg actcccggca
    12361 gaatcatcac atataaaaat ttattcgttt tgattcctgc aattattttt tttcgtcttc
    12421 tgagcttttc ttcttttttc gatagcgtca gttgaaatgg ttcttgtttc tttacacttt
    12481 ccataagccc ctccttagtt ttgtgaatgt ttgctgctta tgacttcatg atagataact
    12541 atctgaatta cgtatataat catttttacc gtaagtaaaa acgcttacat agcaatgatt
    12601 tttgtgctat acaaaacgat aaagtgtatt ttttcacaaa taaactacgc atagcaaacc
    12661 ttctattttt ttctatctga cacttagcat aggaaatgat tatatacgta atctttcacc
    12721 gatgataaat tgaacatata gaaacagaaa catttaattt attttgagga ggagcctatc
    12781 tatatgaaaa agaaagtctc tataggagaa agaagtttta cacttttcaa caatacgttt
    12841 ctaattttgc tagctttgat ctgtatcgtt ccttttttaa atatcatcgc tacatctttt
    12901 gcttctacac aagaagtcgt cgcgaaaaag tttatccttt ttcctactac cttttcttta
    12961 gacgcgtatc gctacattct ttctacgccg accattttta gagcacttgc tgtttcgatt
    13021 ggtgttacag gtgtcggaac gatcgtcagt atgtgtgcaa cttcactgat ggcctacggt
    13081 ctttccagaa agtatttgtt tggtcgaggt ttcgtcaact ttcttgttgt attttctatg
    13141 ttgttcagcg gggggatgat tccaacgttc ttagttgttc gttctctagg gctggtcaat
    13201 tcttactggg ccatgatcct gcctgtggct gttaatgcaa tgaatatgat cattatgaga
    13261 aactttttcc aagccttgcc ggatagtttg gaagaatcag caaaaatgga tggttgcaca
    13321 gattttggtg tatttttcaa aattatgctg ccattagcgt taccgtctat tgctactatc
    13381 tcgctttttt atgctgttac ttactggaac acttatatga ctgctatttt gtatatcaac
    13441 gattcatcta aatggccgat acagattctt ttacgccaaa tcgtcattgt atcaagtggg
    13501 atgcaagcag aaagtagtgc cgttgatgtg attccaccag cacaaacagt taaaatggct
    13561 gttatcgtta ttgcaacagt gcctatgctg attgtttatc cttttgttca gaaatatttt
    13621 gtcaaaggag cactcgtagg ttctgtcaaa gggtgattat gtactgaacg caaaatggta
    13681 gttgtgtcag aagtggtcag cttcaagaaa taagaaggca tttacgaaaa ttgcttctta
    13741 aatttttgtg aaatttcggc ttatttttcg aagaatctac tcctggaaca ccgtgtatca
    13801 gaggttgtga aagagacgtt cagccttgag cgatgataaa aactctcaca aacattgcga
    13861 cgagtaaccc acaggagcaa taagaagcga ttttgaaaaa tagcttccca tatttcacaa
    13921 aattatgcct tattgtcgat agcctgtctc ttgaacgcca tttattcgta aaaaagaact
    13981 gtgacaagac ttttgtcaca gttctttttg cgtgcttagc cttgaaattt aactatccat
    14041 aaatacaaat actagggtta ttctaacttc ttttattgct tgaatcagtc cacaagaaaa
    14101 acagcctctt gattcacaaa tatttttttc gatattctcc cggcgtaatc gattccttct
    14161 ttttaaagaa acgaataaaa ttttgcggat ttcgatattg caatctttct gcaatctctt
    14221 ttacggagag gttagtctct ttcaaccatt tttttgctac ctctaaacga tgattttgaa
    14281 caaaatcacc aaatttttct cctgtttcct ttttaaatac attacttaaa tagttagggt
    14341 tataatgcaa cctatcagcg atcacatcta aagacagatc acgatcatat tctgtgtaga
    14401 cgatatgcat aatattctcc gacaaatttt taaattcatg atcagtgatt gcttgagagc
    14461 attctgtgat tggcagaact aattgttcta aaacaagttt ttctagttct ttgggatgat
    14521 aattgttcag tgcttttaca taaatttgtt tcatgttatc aaagtttttt gaatctactc
    14581 ccaataactg tcctaattga acgagttcat tgatcaagcg gaccgaaact acttcccgac
    14641 tcaaaggatt tttattttgt gcaaacaatg catcaatcaa ttcatgtact aaattcttca
    14701 cttcctgatt tccagaacga atggcttcaa acaatcgatt ttgttcttct atcggatatc
    14761 taataagtgt tttttcatga gcagcaggaa cgatttcctg ataaaaaata attgaacttg
    14821 gaccagtatt tacacgataa tacagggctt cttttgcccg atcaaccgat tgcttgcttt
    14881 cattcagcgt ttcgaaccgg ttactgatgc ctatactgat agttaaattc aagtattctt
    14941 ttacacgttt ttgaatgagt cggcaatact ccatgacttt tttgcttgct tctgcatcat
    15001 ctttatccat tcgataaata gtggcttgca tttcttcatt caaaacgatc ggcagcattc
    15061 gattttcctt tggcacaatt tccgtgatca tgttgttgat cgctaaaagt agcaaatcac
    15121 gctccccacc gtgactatct tctaacagat cgatttgaac caaagctgta taatagcagt
    15181 cattttgaga actataacca aattgctgca accgctgatc tagttctcgc ctttctactc
    15241 gatttctaaa taaactaagt acaaatagcg tttccagctg tggcttttgg gtaactaagt
    15301 gtgcacttaa cgcttctttt tctcctatga tccgactgac tgattgagcg accaaatcta
    15361 gttctttccc actgaatcct tttgatttaa gattcagacg ttcctgtatt tgagaaatcg
    15421 gacgggcaaa tctgtccgaa aataagtagg aaagaagacc aatgagtcct accagtgtta
    15481 aagacactgc aactagcccg attctcagat tgcggatcat tgcgccaatt gcactagagt
    15541 caacttctaa tctgtaaatc caattattat aatctgattt cagtactata tacgttttat
    15601 ctttttctct tatcactttc agctgattgt aatctagatc cacgtccgtt aatacagttg
    15661 gttgctcttt attttttact gtctgagtag taaaaaggtt gccaacttcg ttttcaatag
    15721 aaagtatgtc ttctccactt tcaccaacaa cttgttctat tgtatgtttc tctatcgtag
    15781 caattcccaa tgcatatttt tctttttcaa acattggcaa agaaatcacc atttcgattc
    15841 ctttgttttt cttttgccaa aataaattat ttttgttatt tatgtacttc tgttgatact
    15901 tttcaatttc ctcttcggtc agttgactca acgatccatt gattactccc catttaccag
    15961 ccaaactgac taaatcataa ttattatcat ccataacaat cgtttctata aaatttagtt
    16021 ctttcctgat ttcagaataa gtggagtaat ctgtatatgt cagcggatgc ttgaatatgt
    16081 ccgagaaatt tttggtcgaa ccatacgtag aaaacgaata ttcaatcgtt ctaattttcc
    16141 attcaacgtt agttttaact tgcctgagca aattcttttt gtctgttaaa taactcgatt
    16201 cgatcgattc ataggtattc tggtaaatga ataaactaaa tcctatgaca agtacggctc
    16261 ctaataaaaa catcggtaaa aatattttat aaaatactgt acttttcttc attttcttcc
    16321 tctcctcctt ttaaaataaa aggctttcat ttttctgtaa aattgatact catctaatta
    16381 aatgagcatt ccccgctttc aagaatagca taattaaaaa taaaaatcca agtaattttt
    16441 aacgtgtgca atcttttcat atcaaagatc tattgactta tcgtagttat cgtaatatga
    16501 tgaatgcgtt aacaaaaagt gaaaaagtaa ggaaaatgat ggaatggtaa cgatcaaaga
    16561 tgtcgctaaa gcagctggcg tttctcatac gacagtttct cgtgctttga ataacagcag
    16621 tttaataaaa aatgaaacaa aacaaaaaat acgacaacta gctaaggaaa tgaattattc
    16681 gccaaactat agtgcaaaag gtttagtgaa tcaaaaaaaa tatctaatcg gactattttt
    16741 ctcaagtttg cataaaggaa cttcatcaaa tttcctagca gattccattg caggcatcca
    16801 ttcagtatta gacacaaatt acagtttatc tgttgaaagt atcgatgttg ttgatttaac
    16861 agaaatcaac ctgcaacgct acgatggact gatcgttatg agtcaaagtg atgatgatca
    16921 gcctttcatc gactatctca aagaaaaaga atttcctttc gttgttgtca atcgtcacat
    16981 caatgatcca gcggttatca acgtcactgc cgatgatgcg gccggtgtta tgcaggcagt
    17041 agattacggt attggatgcg gacatacgaa gatcgcttat attggcggaa agaaaaattt
    17101 tcgttcgaca aaagaacggg aaaaaggggt attgaaaagt ctaaaaaaag cgagcatccc
    17161 tgtcaatcca gcctattttt taacaggaga ttacagctta gaaagcggcc tccaaaacat
    17221 ggaataccta ttatcacttt ctgacctgcc tactctggtt ttttgcggca acgacgacat
    17281 agcgattggt gcattgagag ctgtacattt agctggcctc cgtgttcccc agaacatttc
    17341 tctgatcggg ttcgatgatt ctccagtcgt ctcctatctc aatccgccgc ttaccaccgt
    17401 acgtaagcct ttgaaagaaa tgtgtcaaga aggaacaaag cttttactag atttgatcga
    17461 aggcaggacg atcgaacaat ctaaaattga gctctccacc ttgttgatcc ttcgtgagtc
    17521 gatcaattac ttgtaaacta caagtaattt tatataaaaa tgttaacgtg tgcataacac
    17581 agaaaggaag tttatatgaa aaaactgaca aaagaattag tgaaaaaaaa cgaagcacct
    17641 gtaaaagttc ttcagtttgg tgaaggcaat tttatgcgtg gatttattga ttggcagatt
    17701 cagcagctga ataaccaaca attgtttaaa ggtaatgttg caatcattca acctttagaa
    17761 caaggtcttg gaaatatgat gaaccagcaa gataatttat atacggtcat tttacaagga
    17821 ctcagcaatg gcgaaatcgt tgatacttcc gagattatca ccgtagtaga acaaatgatc
    17881 aatccttatg atgaatggga tgcttatcta tctctagcag aaaatgacga cttggcctat
    17941 atcgtctcca atacaacaga ggcaggaatc caattcgtgg agactgataa tttaacagat
    18001 actcccccac agagcctccc gggcaaactg acagcctttt tataccgacg tttcaaatta
    18061 aacaaagctg gctttaccat cattccttgc gaactgattg acagaaacgg cgaacgtcta
    18121 aaagaaatca ttttacgcta cgcagaaaaa tgggatttag aacctgcttt tgttgagtgg
    18181 ttagaaaaag aaaatgtttt ctgttgtagt ttagttgatc gcatcgtccc aggttatcct
    18241 cgcgaagatg caggtgtttt caatcaacgg cataactatg aagatcaatt gatggtcaaa
    18301 gcagaaccat tcatgctttg ggtgatcgaa gggcctaaag aattggagaa tcaactacca
    18361 ttcaaacagg ctggcttgaa tgtcatcatc acagatgata tgactcctta tcgtgaacgt
    18421 aaggtgcatt tgcttaatgg accgcataca gcgatggtcc ctcttgcttt gttggcggaa
    18481 ttggaaactg tagaagaagt aatgaaagat cctttattct ccgcatatgt tgatcagtta
    18541 ttcaatctag aattgattcc tatgctttct ttacctaaag atgaattagc catttatgct
    18601 gaccaaatca aagaacgttt cttgaatcct tttgcccatc acaaattaga agcgatttca
    18661 ttaaatagtg tttcaaagtt cagcacccgt ctgcttccag tgttcaagaa gtatatcgaa
    18721 gaacaaaatc aggtaccacc gttaatcact gtttcactag ctgcgctact tcttatgtat
    18781 cgaggcgatc aagtaaagcc acatgatgat gaaaagacga taagcgaatt tactgatgct
    18841 tggtctgata atggaacagc tattcctcga ttattgcaaa acgctgcgct ttggggagaa
    18901 gatttgtctc aaataccgaa tgtgacagat acagttcaag aaatagctgc aaagatcgat
    18961 caaaaaggtg tccggtcagt attacaagaa atgaaaggaa cgatggtcca tgcttgaaac
    19021 cctgcaacca acaatgaaat taaatcgacg agacagtgtg gtcgtcgcat taacccctat
    19081 tccaaaacaa acacaactga cgattgatca gcaaacgatc accactcttg aagatatccc
    19141 gcaaggtcat aagattgcat tgggtgattt gaaagctgga gacaacgtga tcaaatatgg
    19201 ctatccaatc ggtcatgtga cgacagacgt aactgccggt caatggctgc acactcataa
    19261 tgtaaaaacc aatctttctg gtgaattgga ttatcgatat gaaaaagatg tccatccaag
    19321 tcattacctt tttgagaacc gaacatttca aggttacttg cgaaaaaatg gaaaagtagg
    19381 gattcgtaat gatttgtttt tagtacctac cgttgggtgt gtgaatggca ttgctgaact
    19441 gatcgtcaaa cagttcaagg aaaaacatcc agatctggga cagtttgacc atattacgat
    19501 tttaaagcat ccttatggct gttcgcagct tggaaaagat catcaaaata ctcgagaaat
    19561 tttagcggac gcagtaaatc atcccaatgc cggaggagtt ttagtatttg gattaggttg
    19621 tgagaacaat acagttcctg aattcaaaaa aattttaggt gaatatgatg aagaacgagt
    19681 gaaatttcta gtggctcaag atgtttacga tgagatcgaa caaggtgtgg ctctattaga
    19741 agagctttta acagcggcgg aaaatgacca gcgcatatca gtcccgctct ctaagttgaa
    19801 tgtcggtttg aaatgcggcg gttctgatgg tctatcggga ataactgcta atcctttact
    19861 tggtgcattt tctgactact tgattgctca aggaggcagc acgatcttaa cagaagtgcc
    19921 agaaatgttt ggcgcagaac aagtattgat ggcacgagcg gaaaatgaag aagtttttga
    19981 ctcaatcgtt gacttgatca atgacttcaa acagtatttc ttgtcttacg gggaaccagt
    20041 ttacgaaaac ccatctcctg gtaataaagc aggtgggatc actacattag aagataagtc
    20101 tttaggctgc acgcaaaaat caggaagctc tcctgtcgtt gatgtgctac aatatggcga
    20161 aaaaatcaga aaaccagggc ttagtttatt gcaagctccc gggaatgatc ttgtagctgc
    20221 ctctgctctt gcttcatccg attgccagtt ggtcttgttt accacaggaa gagggactcc
    20281 gtttggctca tatgtcccaa cggtcaaggt atctacgaat acaactcttt ttgaacgcaa
    20341 aggtcattgg atggatttta atgcaggcgt cttattagaa caaccaatgg aagtaatctt
    20401 agaatcgttt gttcaaaaaa tcatcgctgt agctagtgga gaagaaacga aaaatgaaca
    20461 aaatgacgtt cgagaaattg ccatttttaa aaacggagta acactttgat tttcataaaa
    20521 cagtataaaa agaaagaagg aagatttatg tttttatctg atgatttttt attaaaagac
    20581 gattgggcga aaaagcttta ccattcctac gcaaaaaaca tgcccattat tgattatcac
    20641 tgtcacttag atccaaaaga aatttacgaa aacaacaatt tcagcaatct gacagaagct
    20701 tggttaggag gcgatcatta caaatggcgc ttgatgcgag cttgcggcgt gcctgaagaa
    20761 aaaatcacag gaaatgcttc tgattttgaa aaatttttag cttggtgtca aaccgtacca
    20821 aaaattatcg gaaatccgct ttacagttgg tcacatcttg aattgaagcg tttcttcggt
    20881 attgacttgt tgataaatga agaaaatgca gagaaaattt ggacacaagc aaatgaaatt
    20941 ttggcagctc ctgattttcg ccgtcgggaa atcgcaaaaa aatcgaatgt agaagtgatt
    21001 tgtacaacag atgatcccgc agacgacttg cactatcatc agttgcttgc tcatgaagaa
    21061 ccagatttga aggttctacc ttcattccgt ccagataaag cactcaatat tgaaaaagaa
    21121 ggatttaatg catgggtcac actactggaa gaagcagctg accaaacgat caccacctat
    21181 caagaactga tagaagtctt agggcagcgg atagaatact tccatcaaca tggatgtcgt
    21241 gtttccgacc acgctttaga catcttacgc tataaagaag cggatgaaac agtattagaa
    21301 gagatttttc aaaaagcacg agctaacaaa ctattatctt cagatgaaat cgatgcttat
    21361 cgtacagaaa cactgataca tttgacccac ttctatcatt ctcataattg gacgatgcag
    21421 ctgcacatcc atgcttatcg taactgtaat acacaaatgt tggaaaaatt agggcccgat
    21481 accggctatg atggaatgaa tgatctttct ttgactattc ctctgcaaaa attattgaac
    21541 cgtgcagaag aaacggatca gttgccaaag acgatccttt attcattgaa tccgaatgac
    21601 tatcctgcga ttttagcttt gatgggctgt ttccaaaaag aacagaatgg aaaactgcag
    21661 ttaggttctg gatggtggta caacgatact cgagctggta tgcgcgatca attgacacaa
    21721 tttgctgatg gtagtgcttt ggggaatttt gtcggcatgc tgacagattc aagaagtttc
    21781 ctgtcctaca ctagacatga atatttccgt cgtgttcttt gtgaattttt aggcgaaatg
    21841 gtcatgcgag gagaagcacc tgaagatgaa caacttttag gaagtctagt tcaagcgatt
    21901 agttatacaa atgctaaaaa ttactttggc ttttttgcct aattgattaa tagtaacagt
    21961 aacgaatcaa aaacaggata gtgaaataac ggaatccgtt tatccacgtt ttagagaaga
    22021 agacggacca aatttgctcc gtcttcttct cttgctgttc tgcttcattg cctataaacc
    22081 tttatgaaag aagagataac atgcctacta tccatattgc cggtgattct actgcggcag
    22141 aaaaagatga aaacaagcgt cccgagacag gttggggaga aaaaatctct gcttattttt
    22201 cacccgagat ccagatcaat aaccaagcaa agaatggacg cagcaccaaa tcatttatcc
    22261 aggaaggaag gttagcccgt ctagcttcac agtttcagcc gaatgatttc ttatttgtac
    22321 aatttggaca taacgaccag aaaatccaag aagaaagagg tacgcttccc ttcggtagct
    22381 atttaaaaaa tctgcagata ttttttgaat gcgcaaaaaa agcgaacgtc caaatggtct
    22441 tactgacatc tgtgacgcga cgagattact tagaaaacgg aacattgaat ccagatatac
    22501 ttggagacta tccaaaagcg atgcgtgcat ttgctgagaa acaccatatt cctcttttag
    22561 atgtgttctc aagatcacaa gaattgttcc agaccttctc aaaagaagag acaaaaaaat
    22621 tctacctgca tcttatacct gatacttgta aaaattaccc agaaggacta aaagacaata
    22681 cccactttag tccagaaggc gctgataaag ttgcccgaat aattgttgaa ttgataaaag
    22741 aatcccgtct gcctcttgct aattatttac aaaaaggagt ttgagatgtt tccattaaat
    22801 ttaggcatcc gtgcccacga tttagatgtt cgaaatcgtg aagaaatcgt tcaaaaagta
    22861 tcacagcttc acttgtccca tatccaattt gcaccacaaa aagcatttcc tgaacaactt
    22921 aaattaactg acatgacact tgggagtgct gcctattttg gtgaatactt ccaaaaaaac
    22981 aatatcgaac tttccatttt aggatgctat atcaacctat ccagcaaaaa tcaagaagtt
    23041 cgtgcacaag cattggcaac tttcaaacag caaattgctc tgtgctctgc ctatcaagcc
    23101 aagatgattg ctacggaaac cggtagtgtc tctgtcggtt acacaaaaga aaattttact
    23161 gaagaagctt atcagatcgc cagaaattct atcattgaat tagtcgctca tgcagaaaat
    23221 tttggcgtaa ctgttgctgt tgaagcaggg atcaatcatc caatctataa ctatcagtta
    23281 gctaaacgtt taatcgatga agtagcttca ccaaatttaa aaatcatttt agattgtgcg
    23341 aatttgatgc ataaagaaaa tcatgctgat caggaaaaag tgatccgagg tgccttggac
    23401 aatttacatg gttatattac agcgcttcat ctcaaagact atatcatgga aaatggaaag
    23461 atccgcatgg tacctgttgg caagggctgt ttagatttcc gcccggtact gcattatatc
    23521 aaatatgagc gtcctctaat gtacgccact cttgaagcta ctcctgagat ttatgttcct
    23581 gaagcaatag aacacttgca gtttatttac caagattgtt aaggagaaga ttatggagga
    23641 acgaatacag aaaagttatc aaaaatatca tgcaaatgct tttcagacat tgctgcatct
    23701 ttatcaggga aattattggc gttttgccat gtcaggactc ttttttatta tcaaacatct
    23761 tccatcttgg ttaatgccga tcgctattgc caatgtaatc aatgcagctt catcgaaaga
    23821 ttcttctcag ttgcaggcca tttatttcaa cgcttttttg atggcttgtc tgattggtca
    23881 aaatatattg acgaattatt ttcatgtaaa ataccatagc acttcgatcc gtcaagtaga
    23941 agctggacta agagcgaccc ttatcaagaa aatccaagaa ctatctattc cttatcaaaa
    24001 agaattgcaa tctggacgaa tccagtcaaa gatcatccgt gatgtagaag ctatccaaac
    24061 gttgtcctct caagtatttg tcagcggatt aaatatcgct gtcaatttat tcgtagcttt
    24121 aggtatcacg ctttataaaa gtcctgttgt tttcatgttt ttcttattga ctgttcctct
    24181 cgccgcattc gttactctct ttttccgtaa aaggatcaga cagtccaact cagacttcag
    24241 gcaagaaatg gaagagactt ctgcaaaagt gatcgaagtg atcgaattag caccgattgc
    24301 tcgagctcat gggttagaaa aacacgaaac aactcgctta aataagcatt ttactcaaat
    24361 ctatgaaaaa ggattccact tagatatcct gcaatctgtt ttcggatcag tcagctgggc
    24421 tagttttcag atatttcagc tgatttgttt gattttcact gggatattag ctttacaagg
    24481 gcgcatccaa cccggcgatg tcgtgatgta tcagacgtat ttcacgacga tcgtcaattc
    24541 cgtttctgga atgatcacgc tgattcctac gatttcaaaa ggcatggaat ctatcacttc
    24601 tattggcgaa ctgttgattg ctggcgatgt agaaacttat cacggtaaga aaaaaatcca
    24661 acatttggat ggaaattttc gttttgagga tgtttctttt ctttatccgg actcactcaa
    24721 accgattatc gatcattttt ctctagaggt gaaagctgga gaaacaattg cttttgttgg
    24781 gccatctggc tctggaaaat ctacactcct taacttattg attggattta tccagccgac
    24841 ttcaggaaaa atttttctag atgatcagcc tttggatgaa ctggatatgc gaagtgtgcg
    24901 aaattattta gccgttgttc ctcaaaccac tttgttgttc tccgcttcga tcaaagagaa
    24961 catcacttat ggcttgaaaa acgtatcaaa agaacgttta gatgaagtca tcgaagctgc
    25021 gcaactttct tcactgattg accagcttcc tgatggtctg gataccttag taggcgaaca
    25081 tggcaacaag ctgtctggtg gccaaaagca acgtatctcc attgcacgag cgttgatacg
    25141 tcagccaaaa atcattttgc tagatgaagc aacctcagct ttagataacc agtcagagaa
    25201 aaaaatccag caagccctga attacctgac agaaacaccc actactttta ttgtggctca
    25261 tcggctttca acgatcaaag aggcagataa aatcgttgtg atcgatgaag gaaaaatcgt
    25321 tgaaataggt acttacgaaa aactgttagc tcaaaaaggc tatttctacc gcttgaaaaa
    25381 tgaatgacaa aaaataggag ctatgacaag acttttgtcg tagctcctat ttctgaataa
    25441 acggtgttct cctgccaact cctcggtcac aagtcacaat agtcatcaaa catgagaagc
    25501 tgttttttga aattgttcct tgtaactcag agttaaacgg taggctcacg acctcttggt
    25561 aaatggtgtt caagagacag cctatcggca ataagaagca attttgtcat cgctcaaggc
    25621 cgaacgtctc tttcacaccc tctgttgttt cataaaatag ttttattcgc tgtaaaggtc
    25681 gcgaactggt gtcatgatga tacggttcaa gtcattgatg attgtactga agccttgctc
    25741 tttttgcatc aagtcattga ttacttcttc tgattggatt ttcacagcca tttcttgtgc
    25801 tttcattgca tcttcgtcgc tgaactcttg tccactcatt tgtttttctt gtaattcgat
    25861 ttgcatcgct tggaagtctt tgaacagttt gtgtgcttct tcgtttgctt gtactttctc
    25921 gtacgcttct ttcaacgctt tgaattctgg taattcacgg atctcacgtt cgatttgatt
    25981 cgcgctatcg tagatgtttg acatgttatg ccctcttttc ttttagtagt tactctctat
    26041 tgtaacatgg ggttattcgt tttcaaaggg gctactctcc ttgatatttc cgccagaggt
    26101 ctttagccgc ttcaccaatt ccctgaacgc cttctttcac catatcgccg aagtttttca
    26161 gcccttcttt tgcttgttca ccatattgat tcaagttatc ttgccaattt ccagactctc
    26221 cattagaatt tcctgtgttt ccaacttgat ctgcagctac cacttgtcct cctgtggcat
    26281 aggcatctgc tacactaaat cctgtttttg aagtatacgg aaggatgcct tctgccgctg
    26341 atttgaagac tttcccgaca acatttccac ttgtcccttc caaatagtgc agcttgctgg
    26401 attcttcgta acccatccat gtcgaaatca ctacgtctgg cgtgtaaccg atcatccatt
    26461 ggtcatttgc ttttgtggca tcgaaattag tttctgtggt tcctgttttc cctgctatcg
    26521 tataacctgc aggattcgca gcagccgctg ttccatttga aaatgtacca agaagcatgc
    26581 tggtcatttc atcggctgtt tcctttgata tgacctgttt tggttttttg tttgtgttat
    26641 cgacgatcac cgctccagtc gaatcaacga ttttcgtgat aagatgcggc gtatacatct
    26701 gaccatcatt ggcaaatgca ctgtaagcac ccgccatgat caatggagat actccttttt
    26761 ccagtccacc aagggctaag ccataatact tatcagattt tgctaaaggc aggccgaatt
    26821 cttctgcttt atcatatcct ttttgtaaac cgatctcatg caaaagccat acagtaggca
    26881 aattcaaact ttgtgcaaca gcttgataca tcggtacttc tccttggtat gtcccgtcaa
    26941 aattatgcgc atcgtaataa ctttgcggtt cgtctttcaa gatactggaa ggcttgtatc
    27001 cagcttccag tgctggtgta tatacgctta atggtttgat cgttgatcct ggtgaacgac
    27061 gcatctgtgt agcaaagctg aatcctctaa agacatgatc tcctcttcgt cctactagtg
    27121 cttgtacacc acctgtattc ggatctaaag ctactgaagc agattggacc atggcaccat
    27181 ctgaagcatt tgctgggaat aatgcactgt tttgataagt agcttctaat tgcttttgat
    27241 aatcctgatc taaagctgta tagattttat agcctttatt catgacatct tcttcatcaa
    27301 gaccatactc gttaactgct tcatcgatca ctgcatcaaa ataataagga taacgataac
    27361 cagaatctga attatcgtat gtgtcattaa gatacgcgct gatatctgtc tggctttctg
    27421 cttgttcttg ttcttttgtg atttttccgt tttcttccat tacacttagt actgtgtttt
    27481 tccgatcatt cgcatttttt gggtgatcga tcggattata aataccagga cctttcagca
    27541 ttcctgccaa agtagcagcc tcagacagcg taacattcgt tgcatctact ccaaaatact
    27601 ttctggaagc gtcttggaca ccccataccc cgttgccaaa ataggcattg ttaagataca
    27661 tggtcaagat ttcttctttg ctgtacttct tttcgatctc gattgccaaa aataattcct
    27721 tcgcttttcg atcaaacgtt tggtctaatg tcagatacgc atttttggca agctgctgag
    27781 tgatcgtact tcctccgccc cctgatgttc cgccagacaa gaccattcgg actgctgcac
    27841 gggcaatccc tttgatatca aacccgtgat gctcgtaaaa attcctatct tctgtcgaga
    27901 ttactgcatc ttggatatag ggagaaatcg cgtttaattc tacatatgtt cctttttggg
    27961 cgtacagtgt gccagcttct tcgtttttct tatcataaat gactgtcgat tggcttaatc
    28021 ctgattttaa cgtttcgaca tttgcttgtt ttgctaaaat aaagaggtag atacttgtca
    28081 ctaagacaca gatcaaacca atcagcaaaa tcaatttgtt cacatgatat tttttccaga
    28141 tgcgctttcg ccatcgatgg aattgtccga gatatggtct gatccaacgc caaaaactga
    28201 tcaaagcagc ttttatcttt tctaaaatgc tttgaaaatc catttgcttc ttctcctgtc
    28261 tatttgattc aatcgtctta ttttaccata gtgaaagagt gttttcttcc tgcttaagtt
    28321 gtagttcaat atgttttttt acgttagtgc aagtagagta acataagaat gaacagaaaa
    28381 atgtaagttt tttcttaaat atgttttaaa aatggtacaa ttagtctgcg attgcactga
    28441 aaggagtaca cttgtgaaaa tcacgatcac tttacctatg aaccatcctg aaacaacaat
    28501 tcgcgacttg cttgaaaagg aatggttagt tcctagaaag gttcgacact ttttgcgtat
    28561 acgtaaaaac gtttggatca atcaacaacc tgctttgttc catcatgaag taaagggcaa
    28621 tgaccagatc acattgttat tcgaagaaac agattataat tatcaagaag ttcaattagg
    28681 agatgcgaag cacatccgtg ttttatatga agatgagcat ttgattatag tggataagcc
    28741 tgcaggaatg aagactcatc caaatgaacc gacagaaaac agtaccttac tcaatcattt
    28801 agctgcttat ctcgacacta aacagcaacg accttacgtt gtccaccgct tggacaaaga
    28861 aacaagcggt gccgttttat ttgcaaaaaa tccatttgtc ttgccgatac ttggaagaat
    28921 gttggaacaa aaaatgatct atcggagata tcaagccatc gtatggggag aaattgccaa
    28981 agatatgacc atcaccaata aaattggacg agatcgccat gatcggagaa aaagggtcgt
    29041 agatgataaa aatggtcaga gcgccttgac acatgtcact gtcaacaaag tactttctga
    29101 acaaacacaa gtctattgtg ttttggaaac cggacgtaca caccagatca gagttcattt
    29161 agctcataag ggacatccga tcgttggaga ccctctctac caaaatagaa cagctgaacg
    29221 actgatgctc catgcattag aactgcacat gatccatcca tttactcaag aaaaaatagt
    29281 cggtaaagct ttacctggat tatgggaata gaaataaaaa agaacagagt cttatcagca
    29341 gataatgata agactctgtt ctttttgtgt aatttgtctg cttacttttg atgttcgtgt
    29401 tctgctaaca gcaaactacc gataatccca ctttcattcg gcagtcccca acgaacgata
    29461 tattcactta cttgaggtgt ttccatataa ccacccatca attcaacaaa ttgctgacgg
    29521 attttttgaa ggagatgatc ctgattcatt accccaccgc ctaaaataat cttttcaggt
    29581 gctaacgtca acgtatagtt gatcagtgct tgagcaatat aataagcctg aatagcccaa
    29641 acaggatgat cttgcggcag atcttgccct ttgatccctg tgcgtgcctc aatagacggt
    29701 cctgctgcta atccttctaa acaatcttta tgataaggac aagtaccttc ataatggtca
    29761 tctggatggc gtttcaccca aatatgtccc atttcaggat gtgcgatccc tgaaaaaatt
    29821 tcttcattga tcacgacacc tgcaccgatt cctgttccca cggtaaggta aatacaacta
    29881 ttttttcctt gtgctgctcc ttgcttcaat tccccatatg cagcagcatt gacatccgtt
    29941 gtccaagcag caggaatatt gtagcgttcc ttgattgtgc caagaaaatc gaattgtgcc
    30001 cacccttttt tgggagttgc caaaacgtat ccgtaattat cttttgtttt gtcgatacca
    30061 atcgggccga atgagccaat cccgattccc ttcaattcat aacgatcaaa aaattcaaat
    30121 acctgatcca atgtttcttc tggtgttgtg gtagggatac ttaattt
//
LOCUS       DAZKOW010000023        28601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000023 DAZKOW010000000
VERSION     DAZKOW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.023"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            235..1875
                     /locus_tag="ACG912_001044"
     CDS             235..1875
                     /locus_tag="ACG912_001044"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFQ0512381.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2029..3351
                     /locus_tag="ACG912_001045"
     CDS             2029..3351
                     /locus_tag="ACG912_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFQ0512382.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3413..3844)
                     /locus_tag="ACG912_001046"
     CDS             complement(3413..3844)
                     /locus_tag="ACG912_001046"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0512383.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3908..4744)
                     /locus_tag="ACG912_001047"
     CDS             complement(3908..4744)
                     /locus_tag="ACG912_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFQ0512384.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4934..5236)
                     /locus_tag="ACG912_001048"
     CDS             complement(4934..5236)
                     /locus_tag="ACG912_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFQ0512385.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="ACG912_001049"
     CDS             complement(5262..5693)
                     /gene="ruvX"
                     /locus_tag="ACG912_001049"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFQ0512386.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5690..5956)
                     /locus_tag="ACG912_001050"
     CDS             complement(5690..5956)
                     /locus_tag="ACG912_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFQ0512387.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6210..6935)
                     /locus_tag="ACG912_001051"
     CDS             complement(6210..6935)
                     /locus_tag="ACG912_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFQ0512388.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7301..7371)
                     /locus_tag="ACG912_001052"
     tRNA            complement(7301..7371)
                     /locus_tag="ACG912_001052"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7337..7339),aa:Gly,seq:ccc)
     gene            7488..8240
                     /locus_tag="ACG912_001053"
     CDS             7488..8240
                     /locus_tag="ACG912_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0512389.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8256..8735
                     /locus_tag="ACG912_001054"
     CDS             8256..8735
                     /locus_tag="ACG912_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFQ0512390.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8986..10566)
                     /locus_tag="ACG912_001055"
     CDS             complement(8986..10566)
                     /locus_tag="ACG912_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFQ0512391.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10559..11302)
                     /locus_tag="ACG912_001056"
     CDS             complement(10559..11302)
                     /locus_tag="ACG912_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512392.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11478..12254)
                     /locus_tag="ACG912_001057"
     CDS             complement(11478..12254)
                     /locus_tag="ACG912_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0512393.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(12502..13338)
                     /locus_tag="ACG912_001058"
     CDS             complement(12502..13338)
                     /locus_tag="ACG912_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFQ0512394.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(13349..14278)
                     /locus_tag="ACG912_001059"
     CDS             complement(13349..14278)
                     /locus_tag="ACG912_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFQ0512395.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            14470..14823
                     /locus_tag="ACG912_001060"
     CDS             14470..14823
                     /locus_tag="ACG912_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFQ0512396.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(14897..15553)
                     /locus_tag="ACG912_001061"
     CDS             complement(14897..15553)
                     /locus_tag="ACG912_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0512397.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            15779..17713
                     /locus_tag="ACG912_001062"
     CDS             15779..17713
                     /locus_tag="ACG912_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0512398.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(17799..19022)
                     /locus_tag="ACG912_001063"
     CDS             complement(17799..19022)
                     /locus_tag="ACG912_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0512399.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(19016..20887)
                     /locus_tag="ACG912_001064"
     CDS             complement(19016..20887)
                     /locus_tag="ACG912_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFQ0512400.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(20908..22026)
                     /locus_tag="ACG912_001065"
     CDS             complement(20908..22026)
                     /locus_tag="ACG912_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFQ0512401.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(22109..22942)
                     /locus_tag="ACG912_001066"
     CDS             complement(22109..22942)
                     /locus_tag="ACG912_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0512402.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(22935..23852)
                     /locus_tag="ACG912_001067"
     CDS             complement(22935..23852)
                     /locus_tag="ACG912_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0512403.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(24079..25203)
                     /locus_tag="ACG912_001068"
     CDS             complement(24079..25203)
                     /locus_tag="ACG912_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0512404.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            25386..26294
                     /locus_tag="ACG912_001069"
     CDS             25386..26294
                     /locus_tag="ACG912_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0512405.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            26435..28048
                     /locus_tag="ACG912_001070"
     CDS             26435..28048
                     /locus_tag="ACG912_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0512406.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         8892 a         5622 c         4868 g         9219 t
ORIGIN      
        1 ctatttatct atctaacata tcctttattc ttctactttt ctttctttag catataagag
       61 atacgcatag taatcaagca aggattgttg atataaaaag aacaaatacc gctattttga
      121 tttcttttga ctctattact catataaata aaatttatta tacctgctct ttttgactat
      181 atcctcatct gactcctata cttcgtttgt attaaaactt aggaggaatc attcatgctt
      241 acagggctaa acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa
      301 gaacgggcaa aatacggaat cactggtatg cttccaagta ctgtccaaac attggaacaa
      361 caatctgtcc aagcttacgg tcaatactta agcaaacaga ccgatttgga aaaacgtatc
      421 ttcttaatga atctattcaa tacgaaccgg acattgtttt ataaattgat gggcgaacat
      481 ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac
      541 aacgaaattt tcttgaaacc acaagatgct gtatttcttt caatcgacga accagaaaat
      601 atcaaagcaa gtttgaaaaa cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca
      661 gatgcagaag gaatcttagg tatgggtgac tggggtgtga acggtgttga catcgctatc
      721 ggtaaattga tggtctacac tgccgcggct ggtatcaatc ctgcacaagt cttgcctgta
      781 tcaatcgacg ctggtactaa taacgaagaa ttattgaata atccattata cttagggaat
      841 cgtcatgctc gtgtagaagg cgaaacttac tacgaattca ttgatcaatt cgttcaatca
      901 gctactgaat tattcccaga attgttatta cattgggaag attttggacg aggaaatgct
      961 gcaacgatct tagaaaaata cgaagataaa atcactactt tcaatgatga tatccaaggc
     1021 acaggaatcg ttgtactagc tggtgtatta ggtggattga atatatcagg tgagcaattg
     1081 aaagatcaaa cgatcttgac atttggtgct ggtactgctg gtgtaggtat cgcaaacatc
     1141 cttttagatg aaatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat
     1201 caagtagata aacagggttt attgtttgaa gatacaaaag gattgactcc tggtcaaatc
     1261 ccattcgctc gtaaacgctc agaattcact aatgctgatg aattgacaaa cttagaagca
     1321 gttgtaaaag caatccatcc aacgatcatg atcggtactt ctacccaacc tggagcattc
     1381 actgaagaaa tcgtgaaaga aatggcggct cataccccac gtccaatcat catgcctttg
     1441 tctaatccaa caaaattggc agaagcaaaa gcaaaagatt tgattgaatg gacggacgga
     1501 aaagctttag ttggaacagg tatccctgct gctgatgtgg aatataacgg tgtaacttac
     1561 caaatcggtc aagcaaataa tgcgttgatg tacccaggtt tagggcttgg tttgattgct
     1621 tcaacagcta cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga
     1681 atcgttgatg taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa
     1741 ttctctcaaa cgattgctga aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt
     1801 gaagaaatca cggatatcaa agaagctgtt gaatctgcta aatgggttcc tgaatataaa
     1861 tctttagaag attaagattg ttatagtaaa agaacataca gaaactttgt atgacgtcta
     1921 aaaagaaaaa agcgactgcg gcaaaacagc agccagccaa ctactgctct tttgccgctt
     1981 ctctttggct tttttcatac gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa
     2041 acaacaaccg caactgcagt tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt
     2101 accggcgtaa gtttgcccct ttatttgatc atggtccttg tcctgatcat tgcaatggct
     2161 ttaggaaaat taccgacgaa tatgattggc ccaatcgctg ttttggtcat tttcggtaat
     2221 ttatttcatt ttatcggaac aaaaatccct atagtcaaaa gttatttagg tggtggctcc
     2281 gtttttgcga tctttgcctc tgccgcgatt gctacatttg ggatcttacc tgagtatgta
     2341 gtaaaatcca cagaaaactt tgtcagcaat atgggctttt tagactttta tattgctgcc
     2401 ttgattactg gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt
     2461 tttattcccg ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt
     2521 atgcttatcg gaaatggttt tgcaaattcc gttttatata tctcattgcc cgcaatggct
     2581 ggtggtgttg gagccggagc tgtaccttta tcaacgattt atgcaaatgt tttaggtacg
     2641 gacgcttctt ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcctagct
     2701 atcattggtg cagcgttagt tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat
     2761 ggtaaattaa tggaaaaagg cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc
     2821 attccacaat taggtgtcgg tttgatggta tcattgactt tcttcatctt aggacaaatc
     2881 tgtaactatt ttgttcctaa agtccatgct tacgcattca tgatcattat cgttgttatc
     2941 tgtaaaatcg caaacctatt accagaatat tacgaagatg cagctatcat gttcaacaac
     3001 ttgattgtca aaaacttgac agcagctgtt cttgccggta tcggtattgc gttattgaat
     3061 ctaaatgtat tggcttctgc tttgacatgg caatttgttg tcttatgttt gacaagtgtt
     3121 atcgcgattt cagtagtttc aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa
     3181 tcaactatta ctgctggctt atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc
     3241 ttatctgcag ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcggcttggt
     3301 ggtgccatcg ttttgatcat ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa
     3361 atacctgatc atcaagtatc ggcttgatga ccaggtattt tttgataaaa atttacagga
     3421 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgcgttca taaaatcggt
     3481 gtgcttctac tctgtattct gcagaattaa gtcttattgc tgatattcct cgtcttttcg
     3541 cttcttctac gccagtcatc aaggcctttc cgattcctct tttttcagca tgtttagaca
     3601 cagcaagtgc cataatattg aacatcggat cgctgaaaat cgattcatag gactccgctt
     3661 gtacatagcc agctgctttt ttagaagtag gatcttcata gaccaagaaa aaattttttt
     3721 ctggctcctt caacagtttc ttcatttgtc tcaccgtaac ggttaatggt acatcatatc
     3781 ctaattgttc cgcattgatt tctcgtagtg cttctgcatc tcctaaacgc atttcccgca
     3841 acatccaaat caccagccaa tttttatttt tttaaatgaa tttttttatc acagtatact
     3901 ccttttttca cttaaaaaaa gacttttcag tgattttgac ttgcttgaat ccagatcctt
     3961 ctaatgtttg caagatgacg aaagcattgt aaagattgat caaaagaacc acgatgacaa
     4021 tcgcttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
     4081 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
     4141 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
     4201 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
     4261 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccac
     4321 atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga
     4381 agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct
     4441 cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa
     4501 cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt
     4561 gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc
     4621 ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca
     4681 tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa
     4741 tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta
     4801 ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaattta
     4861 gtgctaaacg aaaaaaccgg gctctacttt tcagagagtc cagcttttct tacaccttat
     4921 cgattctttt gttctattct tcttccgcca tgaatgtatt gaatacttct tcgatcatat
     4981 cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct tcgttttcga
     5041 tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagct ggatataaaa
     5101 gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagcaagatt tcgtataatg
     5161 tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcgtgt tcgtgggaat
     5221 ggttatgttc tgtcataaga ttacctcttt tctttttatt attaaaataa ttgcttacct
     5281 gagccgtcta aataattttg taaaatcatc acagctgcta atttatcgat gacttttttt
     5341 cgtttcgctc ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt
     5401 agtcgttcat cttgataatc aaccggcaag gagaatagtt ctttgatctt ctctccatat
     5461 gacatggaag cttctgccct tggtccgatg gaattgttca tattcttagg taatccaact
     5521 acaaaacggt ctacttcgta ctgcgccact aattccttca accgatcaaa accaaactct
     5581 tcttctgcct cattgattcg aatgatttct actccttgag ctgtccagcc aaagggatcg
     5641 ctcacagcaa cgcctactgt cttagaaccg acatccaagc ccattactct cataaattca
     5701 ctccatgatt tgagaggtag tctttgacaa ttacttccat gatttcatcg cgttcgtgac
     5761 ggcgaatcaa atttcgtgca tcacgataac gaggaatgta ggccgggtct ccagaaagca
     5821 agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa
     5881 tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt
     5941 catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct
     6001 acacccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa acttctgaat
     6061 aaattaagaa acacgatttg tctgattttt ccttttttct gtggcttttt caaaagccct
     6121 ctttccataa agtatacgct atatttcttt gaaagtatgt ttttacacaa ttttttcata
     6181 aagttcataa aaaattcgaa ttgctgctac taatttttcg ttttcgttgc ttgccctgtc
     6241 tcataattga tggtgtagcc atcttgaaca ttgtagataa agacattgaa ttcaattttt
     6301 ttgtcttcaa tgctttctgc ttccaactgc acacctcttg ctaccagttc atttccctcg
     6361 aaatgaggag tgacacgata gcgtacatga tgatttgttt ccttgatata tgccgcaata
     6421 tcattttcgt gggcaagcat gtacggagca ttcaacgaac gtgtccctgt cattaagttt
     6481 ttaatgttat tattttgacc agtcaactgg aaaccaatca aatggctgcg gttgtataac
     6541 cattgtccgt cacttaattt cttttgtttc caacctgtcg gatcaatata caaaggttct
     6601 ctcttttcag taggaaacaa gtcttttccc aacatcgcat tggcttgtcc tactctgttc
     6661 aatttgtcta tatttgaata gctttcccat gtccctttgg aaagactaag atcctctttt
     6721 gtaaactccg gctgattccc attgatttct atttcttgat gttttccatc atattctggc
     6781 acatccgcta cttgagaagc ttgcggtgtg gaatcaaaaa tggttgtaat ttgttggaag
     6841 atatctgaat ctttttgtgt ggtggaaata ccgagtgccc ctgcaatcac agcagcgacg
     6901 atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta ctaacagttc
     6961 cctaaatctt aacatatgtt cccctataaa taaatactgg aaagaaatag aaacagtaaa
     7021 aaaataaaac cagtttttag acggcttaag aatacaaaaa aagagacgga ttcatcgtct
     7081 ctactcaatc acaaaacact atttacaagg tatttattat cattcaaaaa atttttctta
     7141 atactcaaaa ctaaacggct tctgtacgac ctcttggtaa acagtgttca aaagcttgca
     7201 tctgcggtaa taagtccaac caagtcaaaa acataagaag cttttttgct tgactattct
     7261 tattacttga tgcagggcag ctttttcaca gcctctttta agcgggtgac gggaatcgaa
     7321 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg cgatacaaaa
     7381 acagtatacg cctctttcac tccttcctca agttttttta ttctttacgc ttccgatatg
     7441 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     7501 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     7561 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     7621 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     7681 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     7741 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     7801 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     7861 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     7921 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     7981 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     8041 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     8101 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     8161 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     8221 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     8281 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     8341 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     8401 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     8461 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     8521 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     8581 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     8641 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     8701 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     8761 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     8821 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     8881 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     8941 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     9001 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     9061 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     9121 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     9181 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     9241 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     9301 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     9361 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     9421 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     9481 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     9541 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     9601 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     9661 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     9721 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     9781 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     9841 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     9901 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     9961 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
    10021 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
    10081 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
    10141 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
    10201 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
    10261 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
    10321 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
    10381 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
    10441 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
    10501 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
    10561 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
    10621 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
    10681 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
    10741 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
    10801 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
    10861 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
    10921 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
    10981 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
    11041 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
    11101 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
    11161 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
    11221 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
    11281 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
    11341 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
    11401 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
    11461 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
    11521 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
    11581 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
    11641 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
    11701 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
    11761 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
    11821 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
    11881 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
    11941 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
    12001 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
    12061 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
    12121 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
    12181 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
    12241 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
    12301 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
    12361 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
    12421 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
    12481 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
    12541 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
    12601 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
    12661 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
    12721 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
    12781 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
    12841 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
    12901 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
    12961 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
    13021 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
    13081 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
    13141 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
    13201 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
    13261 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
    13321 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
    13381 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
    13441 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
    13501 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
    13561 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
    13621 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
    13681 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
    13741 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
    13801 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
    13861 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
    13921 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
    13981 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
    14041 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
    14101 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
    14161 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
    14221 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
    14281 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
    14341 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
    14401 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
    14461 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
    14521 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
    14581 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
    14641 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
    14701 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
    14761 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
    14821 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    14881 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    14941 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    15001 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    15061 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    15121 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    15181 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    15241 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    15301 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    15361 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    15421 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    15481 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    15541 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    15601 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    15661 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    15721 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    15781 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    15841 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    15901 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    15961 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    16021 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    16081 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    16141 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    16201 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    16261 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    16321 acaccctgac attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc
    16381 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    16441 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    16501 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    16561 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    16621 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    16681 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    16741 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    16801 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    16861 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    16921 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    16981 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    17041 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    17101 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    17161 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    17221 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    17281 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    17341 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    17401 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    17461 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    17521 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    17581 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    17641 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    17701 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    17761 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    17821 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    17881 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    17941 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    18001 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    18061 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    18121 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    18181 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    18241 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    18301 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    18361 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    18421 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    18481 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    18541 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    18601 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    18661 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    18721 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    18781 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    18841 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    18901 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    18961 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    19021 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    19081 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    19141 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    19201 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    19261 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    19321 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    19381 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    19441 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    19501 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    19561 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    19621 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    19681 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    19741 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    19801 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    19861 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    19921 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    19981 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    20041 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    20101 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    20161 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    20221 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    20281 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    20341 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    20401 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    20461 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    20521 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    20581 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    20641 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    20701 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    20761 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    20821 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    20881 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    20941 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    21001 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    21061 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    21121 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    21181 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    21241 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    21301 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    21361 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    21421 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    21481 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    21541 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    21601 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    21661 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    21721 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    21781 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    21841 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    21901 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    21961 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    22021 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    22081 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    22141 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    22201 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    22261 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    22321 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    22381 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    22441 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    22501 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    22561 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    22621 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    22681 tggctgtagc tgaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    22741 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    22801 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    22861 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    22921 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    22981 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    23041 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    23101 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    23161 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    23221 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    23281 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    23341 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    23401 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    23461 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    23521 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    23581 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    23641 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    23701 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    23761 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    23821 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    23881 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    23941 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    24001 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    24061 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    24121 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    24181 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    24241 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    24301 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    24361 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    24421 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    24481 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    24541 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    24601 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    24661 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    24721 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    24781 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    24841 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    24901 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    24961 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    25021 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    25081 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    25141 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    25201 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    25261 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    25321 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    25381 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    25441 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    25501 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    25561 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    25621 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    25681 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    25741 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    25801 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    25861 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    25921 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    25981 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg
    26041 aacaattagc ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag
    26101 tgaatggaaa aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc
    26161 tcgtgctaga aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga
    26221 tccatcattt ttcttcagct tttaaaaaat atacagggtt atccccttca aagtattatc
    26281 tatccttaaa atagaaaaaa atttttaggt tttatccaca aattatttct ctatattttc
    26341 tgtctattca aaataaacaa taattttccg gcacaattat ttgctgtttt ttaccataac
    26401 atagtacgat caacgtagaa aaggagatgt ttttatgaaa gttgttgtca ttggcggtgt
    26461 agctggcggt ccttcatttg ccactcgttt ccgtcgattg aatgaagcgc acgaaatcat
    26521 tatctatgaa cgcggagaaa atatttctta tgcaagttgt gctttgcctt attatttagg
    26581 aggtgtgatc actgaccgtg actcgctgat cgaacgtaca ccagaaatat tgaaaacaaa
    26641 aaacaacatc gatgtcttta ctaaacacga agtaacagca atcgatcctt ctactaagcg
    26701 attaacagtt aaagacctat ccacaaatga agaaataaaa acagattacg ataagttgat
    26761 catctcttct ggtgctagac cagattatcc ggatattccc ggagtttttg aagcagaaaa
    26821 cggctttgtc ctccgtagtg tgacggatgc ggatcgaatc aaatcgttcc ttgaagaaaa
    26881 aaatccacaa catgtcgtca ttcttggtgc aggtgttatg ggtctggaat tagctgagaa
    26941 tttcaagcat cgcggcttag acgtgacttt aatcgatcaa ttgccacaag tcgctttccc
    27001 ttatgatcca gaaattgcta atttagttta tgacaaattg ctgaaagaag gattagccgt
    27061 tcatttagaa acaagagtta ctgagatccg tgataaaggt cgagaaatag tattatcaga
    27121 tggctccgtc ctttctgctg atatgctaat ttttgctgtt ggtgtttctc cgaagaatga
    27181 agtggtgaaa gcaaccggca tacaattatc agatacaggt cagatcattg ttgatgacca
    27241 gttacaaacc aatctgccgg acatctatgc gattggcgat attatcgaaa cgactagtgt
    27301 agtgactggt cagccgatcc aaagtatgct ttccagtgcg gccaatcgtc aaggacatat
    27361 gctggcagat attttaaatg gtacgcctat gcgctatcgc ggatatattg gtgcaggtgt
    27421 cgcaaaaatc tttgatcata cagcaagtta tgcgggaatg acagaacatg aattaaaagc
    27481 atcaggcata acaaattata aaactgtttt tatcactccc tttgaccatg cctatttcta
    27541 tccagaagct acaagattaa atctaaagct gatttttgat gcagatagcg gtcgtatttt
    27601 aggtggacaa gcatttggag aaaaaggtgt cgataaacgg atgggagaac tttctgtagc
    27661 aatcaccgga aacttgacag tctttgattt gcctgatttg gagttgcctt actctccacc
    27721 gtattctact acccgtgacc cgttgaatat agctggttat gtcgcaatca atcaaatgac
    27781 gaatatcgta gaaacgatca aagcaagtga tatacccgaa aacgatttga aagaagcgtt
    27841 ctttttagat atacgtgaac ctaataaagc accttctggc agtatctcgg caacaaaaaa
    27901 tattccaatg aatgagttac gagatcgaat caatgaaatc ccaaaagata aaaaagttta
    27961 cattactttc agaagaggat tgaatactta tacttctgcc cgaatcttgg caggtttggg
    28021 tatcaaagcg attttgattg aagaataaaa aaacgaccgc ggtgggtagc cgcggtctag
    28081 gagttatttt tacttgagga gtaaaaatga aaaagtatat agggttgttg gttggtatgt
    28141 atatagaata gaagataaat gtgaagaaat tgtgcattat ttcttcacat ttattaggtt
    28201 atagtttaag aaagtttaaa cattgcgcaa tttaattatt ttagctgttt cttttcataa
    28261 aaaaacatgt tgtgactctg agacaaatca tagtccatag tgatgtctct agagtaacta
    28321 cgcctaatat tacgaggcat catattaatc tatcagcact agtacctaaa ctagcatccc
    28381 tttcgaatat tttttcttat ataataaagt ttgctttcac tcctctaatc tcaccaaatc
    28441 gactggcttg cgttgtgtga tactaggaac tgattggttt agaacgactt ttctattgta
    28501 atcatatttt tcgccatcct cgttgtcttc taatccttca atgaatgatc cctaatgtgt
    28561 gtattaaata atttaatttt tttgttccta tttatttaac a
//
LOCUS       DAZKOW010000024        28299 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000024 DAZKOW010000000
VERSION     DAZKOW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.024"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..328)
                     /gene="rpmB"
                     /locus_tag="ACG912_001071"
     CDS             complement(140..328)
                     /gene="rpmB"
                     /locus_tag="ACG912_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFQ0512407.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            598..960
                     /locus_tag="ACG912_001072"
     CDS             598..960
                     /locus_tag="ACG912_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0512408.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            1012..2694
                     /locus_tag="ACG912_001073"
     CDS             1012..2694
                     /locus_tag="ACG912_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFQ0512409.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2811..4847
                     /gene="recG"
                     /locus_tag="ACG912_001074"
     CDS             2811..4847
                     /gene="recG"
                     /locus_tag="ACG912_001074"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFQ0512410.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4896..5897
                     /gene="plsX"
                     /locus_tag="ACG912_001075"
     CDS             4896..5897
                     /gene="plsX"
                     /locus_tag="ACG912_001075"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFQ0512411.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            6019..6261
                     /gene="acpP"
                     /locus_tag="ACG912_001076"
     CDS             6019..6261
                     /gene="acpP"
                     /locus_tag="ACG912_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFQ0512412.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6529..7533
                     /locus_tag="ACG912_001077"
     CDS             6529..7533
                     /locus_tag="ACG912_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512413.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7534..8478
                     /locus_tag="ACG912_001078"
     CDS             7534..8478
                     /locus_tag="ACG912_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512414.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8478..9440
                     /gene="opp4B"
                     /locus_tag="ACG912_001079"
     CDS             8478..9440
                     /gene="opp4B"
                     /locus_tag="ACG912_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0512415.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9467..10381
                     /locus_tag="ACG912_001080"
     CDS             9467..10381
                     /locus_tag="ACG912_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0512416.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10407..12188
                     /locus_tag="ACG912_001081"
     CDS             10407..12188
                     /locus_tag="ACG912_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0512417.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12536..13222
                     /gene="rnc"
                     /locus_tag="ACG912_001082"
     CDS             12536..13222
                     /gene="rnc"
                     /locus_tag="ACG912_001082"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFQ0512418.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13242..16823
                     /gene="smc"
                     /locus_tag="ACG912_001083"
     CDS             13242..16823
                     /gene="smc"
                     /locus_tag="ACG912_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFQ0512419.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16832..17641
                     /locus_tag="ACG912_001084"
     CDS             16832..17641
                     /locus_tag="ACG912_001084"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0512420.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17652..18650
                     /gene="ftsY"
                     /locus_tag="ACG912_001085"
     CDS             17652..18650
                     /gene="ftsY"
                     /locus_tag="ACG912_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFQ0512421.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18837..21104
                     /gene="gshAB"
                     /locus_tag="ACG912_001086"
     CDS             18837..21104
                     /gene="gshAB"
                     /locus_tag="ACG912_001086"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFQ0512422.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21193..21645)
                     /locus_tag="ACG912_001087"
     CDS             complement(21193..21645)
                     /locus_tag="ACG912_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFQ0512423.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21795..22745
                     /locus_tag="ACG912_001088"
     CDS             21795..22745
                     /locus_tag="ACG912_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0512424.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22738..23697
                     /locus_tag="ACG912_001089"
     CDS             22738..23697
                     /locus_tag="ACG912_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0512425.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23694..24449
                     /locus_tag="ACG912_001090"
     CDS             23694..24449
                     /locus_tag="ACG912_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512426.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24472..25425
                     /locus_tag="ACG912_001091"
     CDS             24472..25425
                     /locus_tag="ACG912_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0512427.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(26069..26365)
                     /locus_tag="ACG912_001092"
     CDS             complement(26069..26365)
                     /locus_tag="ACG912_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFQ0512428.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26720..27076
                     /locus_tag="ACG912_001093"
     CDS             26720..27076
                     /locus_tag="ACG912_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0512429.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            27086..27985
                     /locus_tag="ACG912_001094"
     CDS             27086..27985
                     /locus_tag="ACG912_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0512430.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT         9387 a         4969 c         6108 g         7835 t
ORIGIN      
        1 tttagaataa ataacttcat aatttgttcg tataacaaaa ataaacgcaa aaaaagggta
       61 ggaatcaaaa gtcccaccct taaaaatttt tattaaataa aaaaccgttc actatctgaa
      121 cggtccaagt atcattcaat taaacgcgtt caacttttcc tgatttcaaa gcacgagttg
      181 acacccaaac ttttttaggt tttccgtcga ccataacgcg aactttttgt aagttaggtt
      241 taacagtacg ttttgttgag ttcatagcgt gtgaacggtt gtttccactt tttgtcttgc
      301 gacctgtaaa gtaacaaact tttgccatgg taatttcctc ctttgccttg atttggagca
      361 atagaataat tcagctcata ccagactaac atatcatata gaatgggaat ttgcaagtac
      421 attttcatag atacaattga caattgagaa gaataattgt aaattaaaca aggttatacc
      481 tgaaattttg caaggttttt gagtactttg ggtaactgcc ttttatttca agttgcgcta
      541 tgataaaata tgatagaaga aaaatgggag ttttttaccc gttaaggagg ctcattcatg
      601 gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta ccaatgatgt aattgcaaca
      661 gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag gaatggccag caaaaatcaa
      721 attaaagata atattactga gattttgcgt aaagaaaatt actcaagagg agtagttgtc
      781 cgccaagaag ataatggtat tgctgtagat gtttatacga ttatcagcta tggtacgaaa
      841 atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat acaatctaga ggcattatta
      901 ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg ttcgtgtact gccagactag
      961 gcaggagact ggctggtaac tcagcagtcg atctgaagga ggattatcta ggtgagtgta
     1021 acagaaataa gtgcaagcca gttccaggaa atggtccagg caggtgcgaa tcgtctgcaa
     1081 gtaaatgcag aatacgtcaa ttcattaaat gtattccctg tgccagatgg cgatacggga
     1141 acaaatatga atctatctat gactagtgga gctaaggcag tagtagattc aacctcagaa
     1201 aaagtaggag aattagcagc aatgctttca aaaggattgc tgatgggtgc gcgcggaaat
     1261 tccggcgtta ttttgtctca attgtttcgt ggattttcta agcaaattcc agatgttgtc
     1321 tcattaaatg cttctgatct tgctgcagcc ttcactcatg gagttgaaac tgcgtataaa
     1381 gcagtaatga aaccagtaga aggaacgatt ttgacagtag ctagagaagc agcgaaagct
     1441 ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag taatgacagc tgttgtaaaa
     1501 ggagcaaagc gtgctttagc taaaactcca gacttgcttc cagtcctaaa agaagtcggt
     1561 gtcgtagaca gtggagggca aggactgctg tttgtttatg agggcttttt aagtgcattg
     1621 aatggagaat tccaagcgga tgaaacatat gaaccttctc cagcagaaat ggatgatatg
     1681 gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg cgacagaaga tatccatttt
     1741 ggttactgta cagaaatcat ggtaaaaatc ggagaaggac ctacagtcga tagtacattc
     1801 gattacgaag aattccgtaa ctatttagat ggtataggag attctttact tgtggtcaat
     1861 gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc caggagaagt cttgaattat
     1921 ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca acatgcgctt gcagcatgag
     1981 acgattttgg aacacgatga agaagtgaac gagtttgcga aaaaagaaat accacgtatc
     2041 ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc aagagctgtt tgaaagttta
     2101 ggcgctaatt atgttatcag tggtggacaa acaatgaatc caagtacgga agacattgtt
     2161 aaagcgattc aagaagtaaa cgcagataaa gtgattgtct tgccaaacaa taagaacatc
     2221 ttcatggcag ctgatcaagc agcagaagta gcagatatcc cagtagcagt tgttccttca
     2281 agaacgattt cccaaggatt gacagctatg ctttcattca atgatcaagc ttctttagaa
     2341 gaaaacaaag aaacgatgac agaaatgttg tctagcgttg tgagcggtca agtcactcac
     2401 gcgatcagag atacctcaat tgacggtgta gagatcaaag aaggagactt tttgggcatg
     2461 atcgatggaa aaatcctgat ctctaaccca gatatcacag caacagcgtt aagtacacta
     2521 gaacaaatga tcaatgagga cacagagatc gtaacgatct tggcaggtga tgaaggaaca
     2581 gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa aacatcctga tttggaaatc
     2641 gagatccacc aaggagatca gcctgtctat ccttatctat tttctgctga ataatttctg
     2701 tgtcagaata tctttctaac acagtattca ttcataaaag gccttcggtt agtgatcagg
     2761 tatgatcgct gaccaagtcc tttttttgtt aaaggaggga ggaaaaattt atgcgttctt
     2821 tagatgatcc aatcactatg ctggcaggag tcggagaaaa aagagcagct agtcttgcca
     2881 gcttgggcat ccaaacgatt gaagacttac tgacttatta tccttttcgc tacgaagata
     2941 ttcaggaaag aaatctaaac gagatccagg atcaagaaaa ggttactttg aaaggaatcg
     3001 tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa aagccgattg caatttcgta
     3061 tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa tcaaccttat ctaaaagaaa
     3121 aagtcatttt atcggaggaa atcgccgtat acggtaaatg ggatgcaaag cgtaaagcct
     3181 tgaacgggat gaagatttta ggttctcaaa caatggatga cttttcgccg atatatcacg
     3241 tcaacaaatc aattcgtcaa acgacattag ttgatctaat caaaaaaggg tttcaagaat
     3301 acggcacatt gattgaagaa aatctgccaa acaaccttgt ggagaaatat cgactgcttg
     3361 accgagcttc tgctgtaaga agtatgcatt ttccaaaaga ccacgaagaa aaccatcaag
     3421 ccaaacgacg tgtcgttttt gaagagttct ttttatttca aatgcgtctt caaggattga
     3481 aaaaagcaga aaaagcagaa acgaatggtt tggagatcct ttatgatgtc caaaaattga
     3541 aacaatttac tcaaaagcta ccttttgaac tgacaaatgc acaaaaacgt gtaacgaatg
     3601 aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg acttttgcag ggagatgtag
     3661 gtagcggaaa aacagtggtt gccgcgattg cgttatacgc taccgttaca gcaggtttcc
     3721 aaggtgcgtt gatggtgcct acagaaatct tggcacaaca acatatggaa agcttgcaac
     3781 agctgtttga tcccaatgaa gtaaccatcg cattgattac ggggtcaacg aaagcaaaag
     3841 aacgacgtga gctccttgaa aaactagagc aaggggaaat taacgtcgtg atcggcacac
     3901 atgcattgat ccaagatgga gtcgaatttc agaatttagg tctagttatt acggatgagc
     3961 agcaccgttt tggcgtgaac cagcggaaag tacttcgtga aaaaggctgg cgaccagatg
     4021 tcttatttat gacagcgact ccgattccta gaacattagc tattacagca tatggtgaga
     4081 tggatgtatc tatcattgat gaacttccag ctggacgtat cccgatcgaa acacgctggg
     4141 taagaacacc tcaattggac agtgtactgg attggacgaa aaaagaactg gcaagaggac
     4201 atcaaatgta tgtcatctgt ccgctgattg aagaatcaga agcattagat gtaaaaaatg
     4261 cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc aaaatatgaa gttggcttgt
     4321 tacatggtaa aatgaagaat caggaaaaag aagtaatcat ggaagccttc aaagacaacc
     4381 agatgcagat attagtttcg actactgtaa ttgaagtagg ggtaaacgtg ccgaacgcga
     4441 ccgtcatgct gatcatggat gcggatcgat ttggattggc acagctgcac caacttcgtg
     4501 gacgtgttgg acgcggaagt gatgcttcct actgtatctt agttgctaat cctaaaaatg
     4561 aactaggtgt ggaacggatg aaaatcatga cagagaccac caatggtttt gtactaagcg
     4621 aaaaagatct ggaattacgt ggtcctggtg aagtatttgg cttccgtcaa tctggtctgc
     4681 ctcaatttgc gattgctgac ttggtgacgg atggaaatgt tttggaggta gccagagaag
     4741 aggctgcagc cctttggcaa ataaaggatt ggcagatgct tcctgagtat cagcctttag
     4801 cgcaaagcct ccaaatagaa aatcaggata atcgttattt cgattagcag actgtgctat
     4861 actttatact gatgacggaa aaaggagtgg aaacaatgaa aattgccgta gatgcaatgg
     4921 gcggagatca cgcacctcaa gcaattgtag aaggcgtggc tttagcgcag caagattttc
     4981 cagaagtaga attcttactt tacggaaaag aagcagaaat caagaaatac ttaacaaatg
     5041 aaaaaaacat cacgattatc catacagacg aaaaaatcaa tagtgatgat gagccagtaa
     5101 aagcaattcg acgaaaaaag actgcatcaa tggtccttgc tgcacaagca gtgaaaaacg
     5161 gtgaagcaga tgctgtattt tcagcaggga atacaggcgc gctactagca gcagggttgt
     5221 tcatcgtagg acgtatcaaa aatatcgaac gccctggctt gatgtctacc ttgccagtag
     5281 tagggaaaga aggtgccggt ttcgatatgc tggatcttgg cgctaacgca gaaaacaaac
     5341 cagaacattt gttgcaatac ggtattttag gttcttttta tgcacgtaaa gtccgaggta
     5401 tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga agcaacaaaa ggtagtgaag
     5461 tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag cttgaatttt attggaaacg
     5521 tagaagctcg tgagctactc aatggtgtgg ctgatgtagt ggtcacagat ggatttacag
     5581 gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa tatgatgaat ctattaaaat
     5641 ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc tatgctttta aaagacggct
     5701 tacgttcttt gaaagcagaa atggattatt ccaaacatgg cggtgccgtt ttgtttggct
     5761 tgaaagcacc agtcattaaa actcatggag caacaggccc cgatgctgtt cgctatacga
     5821 tccgccaaat ccacacaatg cttgagacag atgtagttgg acaattagtc gaacaatttg
     5881 aaaaaagcga agaatagtat ttggatatct gaagcgatcc gttccattga aaaaaaggtg
     5941 gacattcctg aaaaatgcca gtaaaattag ccatggtgta ggtaattgac aagtgtatga
     6001 ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta acaaagtagc gaaaattatt
     6061 tcaaatcatt ttgagataga taccgataaa gtgacggatg aattgaacat caaagatgat
     6121 ttaaaagctg attcaattag tattatggaa ttcgttttag agcttgaaga tgaattcggt
     6181 acggaaattt cagatgaaga cgctgaacaa attgaaacag tcggtggcgc agtagattat
     6241 atcagcagcc atcttaaata atttcaaata aaagcaaaga aggataaaaa gctgcatgca
     6301 gctttttatc cttctttttt taatcttttt gacacgtaaa agtcattttt ctgataattc
     6361 tggcgataaa cagaaattat gttgcaaagt aaagcggttt tctatataat tattaataat
     6421 tcattacaca aagataagaa taacctttgt tataaaacga tttattctgt gtaatgtcct
     6481 tttttattaa gaaaaacttc catttaggaa gcagagaggg gtattcgatt gtccgataac
     6541 caattattag aagttcagaa cctacataca ggattccgac tcaaagatga ctattacgat
     6601 gcagtagatg acgtttcatt tactttagac aaaaacgaga ttttagcaat cgttggggaa
     6661 tctgggtgcg ggaaaagtac cttggctact acgattatgg gattacttga tcctaataac
     6721 acaaaaatta caggtgagat tttatataac gatctgaatt taatcgacct gaacgaaacg
     6781 ctctataata aaatccgcgg aaatgatatc ggaatgattt tccaagatcc gttgtctgca
     6841 ttaaatcctc tcatgagaat cgaagaccag atcaaagaag gactttccta tcacacaaag
     6901 atgaatgcag aacagcgtca agctcgtgca ctagaattgc tagaacaagt agggattcct
     6961 aatcctgcga gagttggacg ccaatatccc catgaactat caggcggtat gagacaacgt
     7021 gtcatcatcg caatcgcaat cgcctgcaaa cctccaatcg tcattgcaga tgaaccgacg
     7081 acagccctag atgtaaccat ccaagcacag attcttgatt tactgaaaga ccttcaggaa
     7141 gaaacaaaaa cagggatcat cttgatcact catgacttag gagtcgtagc agaaatggct
     7201 gatcgagtgg cagtgatgta tggcggacag ttcgttgaag tagcaggtgt aaaagaactg
     7261 tttgatcatc ctaagcatcc ttatacacgc tctttactga actctatacc acaagaagga
     7321 aatcatgatg cagaactcca tgtgatcgaa ggtgtcgttc cttctctgaa aaacatgcct
     7381 cgtaaaggtt gtcggtttgc accacgtatt ccatggatac cagcaagcga tcatgaagaa
     7441 gagcctatcc ttcatgaagt cgcaccgaac cacttggtac gttgctcctg ctataagcat
     7501 ttccacttta gagacgaaaa aggagaggtg tagatgacag aattaatcac aatcaaagac
     7561 ctgaaagttc attaccctat tcgcagtgga ttcttcaatc gagtaacgga tcatgtatat
     7621 gctgtagatg gtgtggattt tattatcgaa aaaggaaaga catatggttt agtgggagag
     7681 tctggctctg gaaaatctac tacgggaaaa gccgttgtgg gattagaaaa ggtaacagcc
     7741 ggacaaatca tgtatgaagg aaaagatgta acgaagcgaa gcaaccgcaa aaaaatgggt
     7801 tataacaagg atgtgcagat gatcttccaa gattcaatgt ccagtttgaa tcctaaaaag
     7861 cgagtgttgg atatcattgc tgaacccatc cgaaattttg aacggttaag cgatcaagaa
     7921 gaaaagaaaa aggtaaaagg actgctagat atcgttggga tgccggaaga tgcgctatat
     7981 aaatatcctc atgaattttc aggcggacaa cgtcaacgtc taggagtagc ccgtgctgtt
     8041 gctaccaatc caaaactgat cgtagccgat gaacctgtat ctgctctgga tctatctgtt
     8101 caagcgcaag tattgaattt catgaaacga atccagcaag aatacggcct cagctattta
     8161 ttcatttcac atgatttagg tgtagtaaaa cacatgtgcg acaatatcgc aattatgtac
     8221 aaaggtcgat ttgtagaaat cggtacaaga gaagacattt ataatgaccc gcgacatatc
     8281 tatacgaaac gtctgctttc agctattccg caaatcgatg tagaaaatag ggaaagccac
     8341 aaagaaaatc gacgaagagt agaacaagag tatatccaaa accaaaaaga atactatgat
     8401 gcgactggtc gagtctacga tttgcgtacg atcacaacta cacacaaagt ggcgttgaaa
     8461 gatggaggtg caagctaatg tggaaaacga ttctgcgacg tgttttattg atgatcccgc
     8521 aagttattat tttaagtgtt ctgatcttct tattagccca agcgatgccg ggggatccat
     8581 ttaccggttt gatcaatcca aatcaggatc cggcagtgat cgaacagatg cgacaagcag
     8641 ctggcttgaa tgacccatgg tatgtccagt acttccgttg gatcggaaat gctttgcaag
     8701 gcgattttgg tcaaagtttc ttatacaaac gctctgttgc tgacttgatc ggcgaacgga
     8761 tcatcaacac tttatatcta tcgatcttga cagttgtcct tacttatttg attgctgtgc
     8821 ctctggggat gcttgctggc cggtatcaaa attcaatctt ggataagatt gtcgtaatct
     8881 acaacttctt tagttttgct gttcctttat ttatcttcgg cttgatcatg ctatttgttt
     8941 ttggttatcg tttaggctgg ttcccaacaa gtggatcaca aacactaggt ttgcaaggtg
     9001 gatttatcac tcaatggata gaccgtttga aatatattct gttaccatca attacccaag
     9061 catttttggc gactgccgtc acaatccaat atctgcgaag tgaagtaatc gattcgaaat
     9121 cgctcgattt tgtcagaaca gcccgttcca aaggggtacc gacgaataaa gtattcagtc
     9181 gacatatttt ccgaaatgca gcgttgccga tggcttctca aatggggtat gaaatcactt
     9241 cgttgattgc tggttctgta gttatcgaaa agatttttgg ttatccggga gtcggaaaac
     9301 tatttattga ttccatcggc caacgggact ataccgtgat cacatcctta gtattgattt
     9361 taggaatcgc gacgctagtg gggacattac tttcagatat catcatgagt atcgttgacc
     9421 cgcgtattcg gattcaataa aaccaatcag aaaggagaat ggatcgatga gtgaaaaaaa
     9481 caatacagca gttcaagaaa gtattcctcc tatggggata cgaatgattg ctcgagaatt
     9541 taaaaaagat aaaattgcta ttttttcatt aggtttgcta gtggttttat tattagtgat
     9601 ctttatcggt gcgatgctga ccgatcaaga caaagtgatg tatgttagta tcttcgacaa
     9661 atacgctgca ccaggaacag tttcgacaca aggacaaaag ttcattctgg gagcagatga
     9721 aggcggacgt gatgtattag gacaattgat cataggtgct agaaactctg ttacgatcgg
     9781 atttgcgatc acgatcctta cttcgatcat cggggttgga ttagggatca tctctggcta
     9841 ctacggaggg atcattgaca acatcttgat gcggatcgtt gattttatca tgattctacc
     9901 gatcatgctg atcattatcg tcttcgtatc gattatttcc agatacaatg tctggtcctt
     9961 tatattcatc atgagtgcat tttattgggt agctaaagcc cgattattcc gaagcaagac
    10021 attatctgaa gcaagaagag attatgtcag tgcctcaaaa acaatgggaa ccaatgattt
    10081 taaaatcatg ttccgcgaaa tcatgccgaa tctaagttca ttaatcatta ctaatttgac
    10141 gatgaacttt gcggcaaaca tcggtatcga gacaactttg actttcttag gtttcggctt
    10201 gccggcaaat gtacctagtt taggtacatt gatcggttat gcaagtaacg gagaagtctt
    10261 agtgaatcgg caatggatct ggttacccgc atcaatcctt attttagttt tgatgttgag
    10321 tatcaattat gttggacaag cattcaagcg ttctgcagat gcgcgccaac ggttaggcta
    10381 gtatagaagc gaagggggaa ataggaatga agaaaacgat ttttggattt gcagcgctga
    10441 tgtctgctgt cgttcttgca gcatgcgggg gcggaggtac agatacctct ggaaacaact
    10501 ctggaggcaa cggcaataac aaagaggaat taacttttcc aattgcaaca aaaaataatg
    10561 atgatgcaat cgaagacggc gaattagaag ctgcggtcgc aacggacagt cagtttaaag
    10621 gattgttcca gtgggagttt taccaagatt catatgatgc agcttttatg gctccttctc
    10681 atgaaccgct atttgcaaac gatgacgatt tcagaatcac agacgacgga gctgcaacgc
    10741 ttgatctaga tgaagatacg aaaaaagcga caatcaaagt aaaagacaac gtcaaatggt
    10801 cagatggtga agatgtaacg gcggacgatg tgattttccc ttacgaaatc attgggaata
    10861 aagattacac tggtatccgt tatgacgaca ctttccgtaa catcgtaggt atggaagaat
    10921 atcatgatgg aagtgccgac acaatctccg gaatcaaaaa aatagacgac aaaacagttg
    10981 aaatcgaata taaagaagta aacccaagca tgcttcaatc gggtggtggt atctggtcat
    11041 atgcagcacc aaaacatacg ttagaaggtg tagcaatcaa agatatggaa tcaagcgatc
    11101 aagtacgtaa aaatccagtg acttttggcc cttattacat gagtaatatc gttgcaggag
    11161 aatcagtaga atacttgcca aacgagcact actggaatgg tacgcctaaa ttgaaaaaga
    11221 tcacgatgaa atctgtaccg atggcaagtg caactgaagc attgaactct aaattatatg
    11281 atatgatttt ccaaatgcca acagatactt acgataccta taaagaaatt gctggctata
    11341 caaacttagg ccgtgaacaa acttcttata cttatctagg attcaagtta ggtaaatgga
    11401 atgcagatac aaagagtgtg gaatacaatc cagattcaaa aatggctgat aaatcattac
    11461 gtcaagcaat gggctatgca ttagataata atgcagtagg tgaacgtttc tatgcaggtt
    11521 tgagaagcaa tgcgacttca ttgattcctc cagtattcca gtcataccat gatgatgaga
    11581 tgaaaggatt cacacaagat ctagacaaag cgaaaaaact attagatgat gctggctaca
    11641 aagatgtgga tgacgatggt ttaagagaag acaaagatgg aaataaacta acaatcaact
    11701 ttgcttcgat gtccggtggg gaaacagcgc aaccattggc agattactat atccaacaat
    11761 ggaaagaaat tggtttggat gtccaataca caacaggtcg tctgatcgaa ttcaattctt
    11821 tctatgataa attagaaaac gatgatcctg aaattgatgt ttaccaagct gcttggaata
    11881 caggaactga tccaaatcca agtggactat ggggaccaaa tgctgcgttc aactacactc
    11941 gttatgaatc agaagaaaat acaaaattga tcaacgatat cagttcaagc aaagcatttg
    12001 atgaagagta ccaaaaagaa gcatatcaaa aatggcaaca atatgcattt gaagaagcat
    12061 ttgcgattcc aacgttgttc cgtaatgaag tattgccagt caacgaccgt gtcaaagact
    12121 gggattggtc ttatgatgta ccaaacccat gggcaaatgt ttctgttaca gcagattcac
    12181 gttcataatc actaaaaatg accgaaaaag ggcgatgtcc tttttcggtt ttttgatatt
    12241 tttctgttta tttctctaat attcaggtca aggcgctgat ttcatgactt ctccaataaa
    12301 cggtgttcaa agtccagttt ctcggtatta agtcaaattt atgcaaaaat atggaaagcc
    12361 attttcacaa aattttttct tcatactcga aactaaacga atttttcaca acttctcgtc
    12421 aatcactagg attattaaag ctttacttac cttttgaccg aaccatttca aatttgacaa
    12481 aaaaccagta taataaggaa gtatcgaaaa aatatccata aaaggaatgt ggaaaatgga
    12541 caaccagctg acaaaagaac taaaagaaaa ctataacatc gtttttcacg atctaaattt
    12601 gcttgaacaa gcttttaccc attcatccta tgtgaatgag catcgcaatc tgcaattatc
    12661 ggataatgag cgacttgaat ttttaggaga tgccgtttta gaactgatgg tttctcagta
    12721 tttattccgt ttatatcctc atttgccaga aggaaaattg acaaaaacac gagctgcgat
    12781 cgttcgcgaa gacagcttgt cgaaatttgc caaagaatgt cactttgatc aatatgtttt
    12841 gttgggcaag ggagaagaga attcaggagg acgtacgcga ccagcattgc tttgcgacct
    12901 attcgaagct tttttaggcg cgttatatct tgaccaggga ttcgacacga cccatgcatt
    12961 catcgaaaaa gtgatttttc ctaaagtcaa agcaggtgct ttttcacatg agatggatca
    13021 taaaacaaaa ctacaagaag tcttgcaaaa atcaggtgat gtatcaatcg aataccgatt
    13081 gatcaatgaa gaaggtccag ctcatgaacg agtattttgg atcgaagttt atgtagatga
    13141 tcaattgatt ggcactggac aagggaaatc gaaaaaatta gcagaacaag ctgcggcgga
    13201 aaatgcattg gcagcactgt aaaagtaagt gagggaaagc tttgtatttg aaacgaattg
    13261 aaatagccgg ttttaaatca tttgcagaca agacagtcat tgattttgaa aatagcgtca
    13321 cagcagtggt tggaccaaat ggcagcggaa aaagcaatat tacagaggct atccgctggg
    13381 tattaggaga acaatctgct aaaagtctgc gaggcggaaa aatgccggat atcatctttg
    13441 ccggttccga tacccgtaag cagctgaata ttgcagaagt cacagtgatt ttagacaata
    13501 gtgatcatta tctgccattg gagtacaatg agatcagtgt aacccgccgt tatcgacgga
    13561 ctggggaaag cgagtttttc atcaataaac aaagctgcag attaaaagat atccaagaat
    13621 tgttcttaga ttctggatta ggaaaagaat cgttctcaat catttctcaa ggaaaagtag
    13681 aagcgatttt cagtagtaaa ccggaagacc gtcgtgggat ctttgaagaa gcagctggtg
    13741 tcttgaaata caagcagcgg aaaaagaaag cagaacagaa gctttttgaa acagaagaca
    13801 acctcagccg cgtccaagac atcatccacg aattggaaga acagttaact cctttggccg
    13861 cccagagtga ggcagcaaag gagtttttac ggctaaaaga aacactgact caaaccgatg
    13921 tctctttgat ggttgctgaa atcaagacag caaaaaaaga ttgggacaac aaacaagcac
    13981 agcttgcgaa attcaatcta gaattaggca aactatctga gtcgatccaa gaacaagaat
    14041 ccattttggc gaagcagcgt aaagaaaatg cgcaagccga tcgtctgatc gaaaaaaatc
    14101 agcaagtatt attagatctt tcggaaaaac tgaagcagac agaaggacaa aaagatgttt
    14161 tgcaggagcg gacaaagcat acacaaaaaa gctcgcaaga atatcaaact tcattagcag
    14221 aagcacaaaa aaaggttaaa cattttgaaa agcttcaaga aagcttgatg aaagcagcag
    14281 cagaaaaaga aacagagatc caaaaagctg aagcaaatct cataaaaaca cagcaagaat
    14341 tagaaaaata ccaaaaatca acgaaagaat tattggcaga attaagagat caatatgttg
    14401 atttgatgca agaacaagca gctgtaggaa acgaactgaa gtatctagaa cgtcaatata
    14461 tacaagaaac agcaaaaagc aagcagacgt tagccaagca atcggaagta gaagcatcag
    14521 tagaccgatt gatcttacaa aaagaagaat tgacacaaaa gcaggcgcag ctaaaatcat
    14581 ctttaactga aacaaaagag aagttagaaa tgatccaaca aaacgggaaa aagttccaag
    14641 aagcattagc aaaagaacag cctaaaatgt atcaattaat gaatcaagtc cagcagctac
    14701 gtgctcgtaa gaaaagcctt caagaaatcc aagaaaacta ttttggtttt taccaagggg
    14761 ttcgattggt cttgcagcat aaacagcagc tttcgggtat tgtcggagca gtggctgaac
    14821 tgatcgatgt tcctgcagat ttcacgttag ctatcgaagc agctttaggt ggggcagccc
    14881 agcatgtcat cgttgaaaat gaaaaagatg ctcggcaagc catcacgtat ttgaaacagc
    14941 aaagaggagg aagagcaaca ttccttccac tgacgacaat caaaccaaga caattaccgg
    15001 ctcatatatt gacacaagca gcagctgtag aagggtttat agggattgcc agtgaacaag
    15061 tcagttatcc cgatcaaatc caaactgtgg ttcaaaacct gctaggaacg atccttttag
    15121 ccaaagactt aactagtgcc aatgcaatcg cgcaaacgat ccgctatcaa tatcgagtgg
    15181 tttccttaga aggtgatgtg atgaatgccg gaggatccat gacaggtgga gcgaataaac
    15241 gaggaaacca aggcagtttg tttgtacaaa atcaagaatt gaaacagttg actagtgaat
    15301 ttgaagaagc agataaacaa ctccaagcac aagaaaaaaa agttcaggag ctgcaacaag
    15361 aaacagctcg tttagccgaa gaacaagaag ttttgcgtac acgaggcgaa caattacgtt
    15421 ttgaagaaca agaagcgaca aatcagctac aaaacatcat aaatgaactt gaacgttttg
    15481 aaaaagaaaa gcaaatctca acttttgaga cacgtgaact gcagcagttt atcgaagatt
    15541 acgaaaagca aacgaacgaa ttgaaagaca aacaaacaga tcttgaatcg caacgacaaa
    15601 aaatcgacga agaaatcaaa tcgttaagtc aagaaagcga ccagatggaa gccagaagag
    15661 cacaagtaca aagtcaaaaa gcacaagaac aagcagatct tgctgtcttg aaagagcagt
    15721 ttaatcatct gcagatccag ctccgcggtg cccgagtcca aaaagcagag gcaaccgaac
    15781 gtcaagaagc gatagaaaag cagttggcga cattgacagc agacttttct gatcatgaag
    15841 tcacagaaga aagtttggaa aaacaaatta acgaattatc tgctcagcga gaaacattaa
    15901 aagccgagtt ggcaaaagcc aaagagcagc gagatagaag gcaaaaggag atcgataagc
    15961 ttgaagcagt cttggcagag cgtaatcgtg agcaaaaagc acgactgtcg gaacaatcca
    16021 aattagaagt ccaaaaagac cgagctgaaa tgattttaga taatcatttg acctatctgc
    16081 agtcagaata tcagctgaca tttgagaaag cttcccaaga ctatcaagaa acaaccgata
    16141 tcgaagacag tagaacaaag gtttcatcct taaaagaaca gattgaaaaa ctgggaccag
    16201 taaacttaaa ttcaatcgaa caatacgaac aagtcagtga aagacataca ttcttagcaa
    16261 cacagcgaga tgatctgtta gcagcgaaaa accaattgtt tgaaacgatg gatgagatgg
    16321 atgatgaagt gcggacaaga ttcaaagaag tatttgaagc tatccgtcaa gaattcaaaa
    16381 ttgtctttcc aaatatgttt ggcggcggta aagctgaatt agtactgacg gaaccaactg
    16441 atttgctgaa aacaggtatt gagattgaag tacaacctcc tggcaaaaaa ctgcaaagtc
    16501 taagtttgct ttctggagga gaacgagcac ttacagccat cgcgttgctc ttttccatta
    16561 tccgtgtctg tcctgttccg ttttgtatct tagatgaggt agaagcagcg ttagatgaag
    16621 ccaacgtgaa acgttttggt cgttacttga gcgatttcca agatgacaca caatttatcg
    16681 tagtcacaca ccgcaaagga acgatggtcg cagcagatgt actgtacgga gtgacgatgc
    16741 aggaatccgg tgtatcaaaa atcgtttctg tacggatgga agatatcaat gaagaggcga
    16801 aaatcaacga aacgaaagtg taggaacgaa aatgatcaaa ttagtagcaa tcgatttgga
    16861 cggtacatta ttaaatgaac aaaagctgat ttcagatgag aataaacaag cattagcaca
    16921 agcaaaacag caaggggtca agatcgttct atgtacagga cgccccttag ctgcgatggc
    16981 gcattatctg caagaattag gactggtaga tgaaggtgac tacagcatca ctttcaacgg
    17041 cggtttggta caaaaaaatg atactggtga aatcatggaa aagaaagtga tggaagtatc
    17101 agatatccaa cgcttataca aactggctca gcaattggat ctgccactag atgttttgtc
    17161 tgacaatgtg gttttacagc tgcctagtgc cccaaggaag caatccttgt acaatgtgtt
    17221 gaataagctg ctgcaattcc aaccagcgga tataagcgat attacagatg aatttgtgct
    17281 taataaggca gtaatcgctt atgaccaaga agaattagat cctaaaatca aagaaatacc
    17341 agcagaattc catgaaatgt atgagatcat caaaacaaga agtatgcttt tggaatttat
    17401 gccaaaaggt gttacaaaag cttatggtat ttctttattg gcaaaagatc ttggtttgaa
    17461 acaagaagaa atcatggcga tcggtgatga ggaaaatgat ttaccgatga tccaatatgc
    17521 tggactaggc gtagcgatgg agaatgctgt gcctttcgtc aaagaagcag cagatttcgt
    17581 gacagccagc aatgaagaaa atggcgtagc caaagtgatc caaaaatttg tattagagta
    17641 gggggagtca aatgggattt tttgataaaa tcaagcgtgc ttttactggc gaagagaaaa
    17701 cagaagaagt tgaagtacag gagaagtacg acaagggttt agaaaagacg cgtaaaacat
    17761 ttggtgaacg gatgaatgag ttgtttgcca attttcgaac agtcgatgaa gacttttttg
    17821 aagaattaga agaaacactt atcggtgcag atgtaggttt tgaaacagcg atacgaatca
    17881 ccgaagcatt acgtcaagaa gtgaaactca gaaatgcgaa aaaatcggca gaagtccaaa
    17941 atgcgatcat cgagaaactt gtggatctct atgaagcgga aggaatcaat gaagtaaatg
    18001 aactaaatat ccaagaagac ggactaacag ttattttgtt tgtcggtgtt aatggtgtag
    18061 ggaaaacaac aagtattggt aaacttgccc atcagttcaa aaacgaaggt aaaaaagtat
    18121 tgatggcagc agcagatacc ttccgcgctg gagcaatcga ccagttagtt gtctggggcg
    18181 aacgcgctgg tgttgaagtt gttcgtggca atgcaggagg cgatccagca gctgtcgtat
    18241 tcgatgcttt ggaaagagca aagacagagc aggcagatgt tttactagtt gatactgcag
    18301 gccgactgca aaataaagtg aacttgatga acgaattaga aaaaataaaa cgagtaatcc
    18361 agcgggaatt accagaagca ccccaagaaa cacttcttgt attagatgca accactggtc
    18421 aaaacgcgat ggttcaagca aaacaattca aagaaacaac agatgtgacc ggtcttgttt
    18481 tgacaaaatt agatggtaca gcaaagggcg gtatcgtact tgctatccgt aatgaattgc
    18541 atttaccagt gaaattggtc ggtcttggtg aaggaatcga tgacttggag ccgtttgatc
    18601 caaatgattt tgtcgttgga ttgttcaaag gattactaaa agaagaataa cccatgtcta
    18661 aaaagcagga gctgtgacaa aagttgcagc tccttgtttg aatgattgtt tttaacatag
    18721 ctgtgttttt gatgccttca tttttatact tatttgtaag cggttctcaa acaatttcgt
    18781 tctttttctt agactttttg attataatta gattcatatg aattggaggt cacttaatga
    18841 tgaattttaa gcaattatta ttgcatgtca atgcgcgccc ttttatcgac caagctcgat
    18901 ttggtataga acgggaagga cagcgggttg atcttgcagg aaatctagca aaaaccgatc
    18961 atccagcaat ctttggcgat cgatcctatc acccctatat ccaaacagat tttagcgaaa
    19021 cacaaacaga gatgatcacc cctgttaccg attctattcc cgaattattt cagtatctcg
    19081 ctgctgttta tgatgtgact gctcgttcta taccgaaaga agagatgatc tggccattaa
    19141 gtatgccacc tgccttaccg gaaaaagacg aagagatcat tattgcaaaa ttaaaaaatt
    19201 tcgaagatgt cttgtatcga cgttatttag caaaagaata cgggaagcga aaacagatgg
    19261 taagcggtat tcatttcaat tttgaatttg gcgatgagtt gctaagaaca ttgttcagcc
    19321 atcaggaaga atttcaagat ttttccgaat tcaaaacaga actttatttg aaaacagcga
    19381 gaaactttat gcgctatcgc tggatgatca cttatttatt cggtgcttca ccaatgagtg
    19441 agaagaacta ctttttagat gaatcacatc cgcaagaacc tgttcgcagt attcgcaaca
    19501 gtgcgctggg ctatacgaat catccaaatg tgaaagtttc ctatgcttct atgaaacagt
    19561 atttagcaga tatcgagcga atgatcgaag aagggaaact ttcggaggag aaagaatttt
    19621 atacaccgct tcgtttccga ggcggaaaga aagtcgcaga tttagcaaca acaggtgttc
    19681 gttatattga attgcggaat atcgatttga atccttatgc aagattgggt atcaatccag
    19741 agcaagttcg gttcttacaa ttattcctga tgtacatgtt atggacagaa gaaaaagaag
    19801 actgtgacca atgggtagca gaaggtacga ctcgaaataa caaagtagca ctagaacaac
    19861 catcagatca aacagaattt catcaagaag gtagagaaat tcttgaagga atgaagcaaa
    19921 tgctggttga attggattgg ttggattctc tttatttagt agaggaagca ttgactcaaa
    19981 tggatcatcc tgaacaaacg ttagcagcga aactctatca agaagcacag ctatcgagtc
    20041 agcaagaagt tgctgtggca ttgggacatc aatattataa agaaagccat gaacgtccct
    20101 atcagttggc cggttttcgc gaaatggaac tttctactca gatatttatg ttcgatgcca
    20161 tccaaaaagg tgtccaagtc aaagtattag atgagtcgga tcaatttttg cgtctgcaat
    20221 tccaagacca tgtagaatat gtgaaaaatg caaacatgac aagtaaggac agctatatcg
    20281 tcccacttat catggaaaac aaaacagtaa cgaaaaaagt cttaaaagaa gctgggttcc
    20341 gggtaccagg aggcgcagaa ttttcatcca tggaagaagc ggtaaaagct tatccgagat
    20401 ttgccgagca ggcatttgtg atcaaaccta aatcaaccaa ttacgggtta ggtatcacga
    20461 ttttcaagga aggagctagt ctggaagatt atcaagcagg tttagccatt gcttttcgtg
    20521 aagacagttc ggttttagta gaagaattta tgccgggaac ggaatatcgt ttctttgtga
    20581 tcgacggaga agtccaagcc atcatgttgc gagtccctgc taatgttatt ggtgacagta
    20641 tccgaacagt gaaagaactt gttgaagaaa aaaatagtga ccctttgcgt ggaacgaatc
    20701 atcgtgcacc tttagaattg atccaattag gtgaattaga acaattgatg ttgaaagaac
    20761 aagggttgac gatcgaatca gttccccaag ccaatcaaat tgtttatcta agggagaact
    20821 caaatatcag tacaggtggt gattcgatcg atatgactga tgaattttct gaagcttaca
    20881 aaaaaatcgc cgtttctgcg gtagaagcac taggagctaa gattagtggt atcgatttga
    20941 ttataccaga taaagaaatc gatcctacaa ctgataaaaa agcatatggg atcatcgaag
    21001 caaatttcaa tccagccatg catatgcatg tttatccttt tgcaggaaaa ggcagaagat
    21061 tgacgatgaa tgtattgaaa ttgttatatc ctgaagtatt ttaaaagaaa aagcttttga
    21121 cagattgtcc tatttgatgt caatctgatc aaaagctttt tttgcatgta aaaatttatg
    21181 tgttttttcg ttttaaaata aacgatccca gaagcttttt ttcttttttg gtttttcttt
    21241 ttcagctggc gaagagtttt cttctgggta ttttttttcg atatcaatca caggttcatt
    21301 tactgcttct tttgctgtta agagcaatcc gaattcctca ggatttgcag tgacgtaatc
    21361 gttgactaca gtgaaacggc tattgttttt cgttgctagc tggatatatt ggttttggac
    21421 agaaaggggt agagccccat tcaagagtat ggaagcatct ggatgttttt tgagttcttt
    21481 taaaaagttt tctttgtttt ttggttggat catttcagca acagtcatac taagatagca
    21541 gcgttccctg aatgtcccta aatatttatg ctgttcttca gggttcacta aaggtgtacc
    21601 ataaagtccc ttatccaaat ggctttttac atcatcttgt gccataaaaa ataccctcct
    21661 tcttctcttt gtctttctta cgattatagc atattcccaa taatacctaa aacatatgaa
    21721 aaagcggttg cttttgttca acgattcact taaaataaga atgaaaatca ttctcaatta
    21781 gaaaggtgga ttggatgcgc tacggacgac tagtagcatt attagttatt ttaagtagta
    21841 tttcattgtt catcggagta tcgaatgtac aattgtcaga actgctccag ttagatgggc
    21901 aaagcttaca aatcttgcta gtgactcgtt tgccacgaac gatttgtttg atccttgtgg
    21961 gagcgaccag tagtatttgt gggttgatca tgcaacattt gacccaaaat aaatttgttt
    22021 caccaacgac tgcgggcacg atggatagtg cgcgtttagg tattttagtg gctatgattt
    22081 tcctacctgg tgcatccttg attgtccgat ccttgacagc attttgcttt gcttttgcag
    22141 gaacattgct ttttttatcc cttacgcgat tgttcctcca gaaaaatcaa ttgatgcttc
    22201 cgttgattgg agtcatgtta gggaatatca ttggttcagt tgctactttt tttgcttatc
    22261 aattccagtt ggtacaaaat atgacttcat ggcttcaagg gaattttgcg actgtgatac
    22321 gaggaaatta tgagttattg tatgctgttg ttccattgtt gctcttggtt tggctatttg
    22381 cttaccgatt tacgatcatt ggtctgggcg aatcgtttgc agtaaatctg ggaatagatt
    22441 accagaagat gcagttttta ggcattggct tagtatcatt ggctagtgca gtcatgttga
    22501 tcatggtagg aagcattcct tttatcggag tgatcattcc gaatcttgtc tctagaatat
    22561 atggagatca agtgcacaaa acaattggcg taactgcttt agcaggtagt ttgttcttag
    22621 tattttgcga tcttgtcgct cgcgtgttga tttttcctta tgaaatacca gtcagtgtga
    22681 tcgttggaat catcggtggg gctatcttcc tttatcttct gataagggga cgacgatatg
    22741 cttaaaaaaa agccatctat ttttattggt atgcttgttc ttgttttatt tgttacagga
    22801 atgtacttga cgatccatac atatggaaac tggcattttg catgtgcatt gaggagtaag
    22861 aaaatttttg catttatcct tgtctcgcta gctgtcagca gtgccacaat cagctttcag
    22921 acaattacgc ataaccagtt tttgactcct tcagtattgg gacttgagtc gttatatgtc
    22981 cttgtccaaa cggtcctttt cttctttatc ggaggagtga cgatgcttca gcagacaggt
    23041 acagtttttt tcttattgaa cgttgggctg atggtcgggc tgagtctatt gttatttttc
    23101 tttcttttga aaaaaatgag agcgaatctt tttttattac tgatgtcagg gatgatttta
    23161 ggtacgcttt ttggtagtgt cagtaccttt ttacaagtca tcatggatcc gaatgaatat
    23221 gatttgcttc aaggcagatt atttgcaagc tttggcaata tccagagtca gtatcttttg
    23281 cctgcttctt tgatcattat cggctgctgg tacctgttat ttcgatatcg acatgtgcta
    23341 gacgttttcc atctcggaaa tgaacaagca gtaaacctcg ggatagacac agatcgtttt
    23401 gaatttttga ttttatgttg tgtcagtctt ttgacaggga catcaacggc tcttgtgggt
    23461 ccagttactt ttttagggtt tattgtcgca aatatcagtt atcgtgtctt ttctacttat
    23521 cgtcatggtc agctatttat cggaagcttt ctggtagcta tgctgtttct gacaggagga
    23581 cagctcgcag tagagcagat tttcggctgg aatacaacag tgagtgtcgt cgttgaattt
    23641 gtcggaggaa tttattttgt cacaaaattg attcgcgaaa ggaagagtat cctatgattg
    23701 atttacaaaa ggtcaataaa ctgtaccatg gaaaatcagt ggttaaagat gtacagttaa
    23761 cgattcctga aaagaagttg actgctttta tcggtccaaa tggtgctgga aagtcaacgg
    23821 tgttgtcgat gatcagtcgt ttgataccaa aagatacagg tgaaatctat ttagaccata
    23881 acgaagtaaa ggcttggaaa tccaatgaat tagccaaaaa attatcgatc ctacgacaat
    23941 cgaatgaaat aaatgtccaa atcaccgtga aagaattagt agaatttgga cgatttcctt
    24001 atacgaaagg aaaattgacg acaaaagacg aggaaatcgt tcaggaatca ctggaacaca
    24061 ttgggttaga agaaatagcg gatcaatcga ttgacactct ttcaggcggt caactccaac
    24121 gtgcgtatat tgcaatggtg ctagcgcaag acacggatta tattttactg gatgaacctt
    24181 tgaacaatct agatatgaat tttgcggttc aaatcatgca gattttaaaa aatttagtac
    24241 aagaaatcgg gaaaacaatt gttattgttc ttcatgatat caactttgct gcaagttatg
    24301 cagacgaaat tgttgcaatg aaagatggac agttatttac gcaaggaacg acccaagaaa
    24361 tgataaaaaa agatgtattg gatgaattat acgaaatgga catacggatt tgtgagattg
    24421 aaggccgcag attctgtctt tatttctaaa actaacatta ggagagaaga gatgaaaaaa
    24481 ttagtagtag gtatcttgat tctagcatca tttggtttag cagcatgtgg aaatagcaac
    24541 aacaccagtc aggcagacac aaaaaaaagc agcacacaaa ctgaaactac attgacgata
    24601 acagatagta atggagacca aatcgaggtg ccaaataatc cagaaaaagt cgtcgtattc
    24661 gacaatggtt ctttagatac gatggatgct cttggtgttg gagataaggt agttggagca
    24721 gctacaagca gccttcctga gtatctttca tcctataaaa aagtagaatc agcaggtgga
    24781 ataaaagaac cagacttaga aaaaattaat cagcttcagc cagatctaat catcatttct
    24841 ggccgacaaa gagattttca aaaagatcta agtcaaattg ctccgaccat tttcctttct
    24901 ttagatgcga aaaatccatg ggaatctttc cagcaaaatg tcactgcttt aggggagatc
    24961 tttggtaagc aagaagaagc taaaactcaa ttagaggaat tatctagtgc aatcgatcag
    25021 acgaaaaaga aagcagaagc tactgacaaa aaagcactag tcacacttgt gaatgaaggg
    25081 caattatcag cctacggatc tggttctaga ttcggtttca tccatgattt atttggcttt
    25141 gaacaagcag atgatcaaat cgaagcatcc actcacggtc aaagtgtttc ttatgaatat
    25201 gtgctggaaa aaaatccgga tatcttattt gtagtagaca gaacaaaagc tattggtgga
    25261 gacgacagca aagatgatat ttctgccaat gaactggtag cacaaacgaa tgctggtaaa
    25321 aatcagcaga tcatctccct tgaaccagat gtctggtatt taagcggggg cggtttagaa
    25381 tcaatgaaac tgatgatcga agatgtgaat caagcattta aatagcataa ataacagagg
    25441 attaaactct catactatgt aacaataatg tttcttacat tcttaaatag caagagccta
    25501 tcctcaaaga agttatcaaa aaccatccgt aaaatcgtat tgatgatttt acggatggtt
    25561 tttatttatt tgagaaatct tttttcaacg tgaattcatt atctacatca tacgggttga
    25621 ttctagaatg gggagtggca aaaaatagtg tgatccattt tttcagcatc aagatcgcct
    25681 cctttgattt gaatcaaagg tacaaagcga aacttagata aaacttaaat ttagatattt
    25741 ttattttgta ccgatctctt ctttaaacga tgttccatct gctgcttctc ggcaataagt
    25801 ccactagact agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaaaca
    25861 aatcgcagtt gtcacaatct cttctaaacg atgttccatc tgctgcttct cggcaataag
    25921 cccactagac tagaaaatat gagaagctat tttctgtcca gcgttcttat tactcaaagc
    25981 aaatcgcagt tgtcacaatc tcttctaaac aatgttcaat cagctgacct tcggtattag
    26041 ctcaaagcac tgatttcaca acctctattt aggcatgtgt ctcagcaagc ggttgatagt
    26101 tgatgatctg catttttcga taaaaatcgg cgggaagctt actccaccgt tttagtaatt
    26161 gatagaaata actctcttct gctttgattt tatactcgct taatatcaat ccaaaactat
    26221 tcgttttttt tgaagctaat tttttataaa tatccaatgc tttggtttta tcttcattga
    26281 caaagagtgt ttttacttca atataagaac cctcgatatt ttgtgtttct tcgataaaat
    26341 agatgttttt tatatattcc ttcatagtga ttcctccaaa gaagtctttt atttgataag
    26401 tttactctac cgaagagttg ttaataatcc gtgaacaaaa agtaattctt tttccaatag
    26461 ttgttaaagg ttttttattt tttatagtat tttttgttat ttaagaaagg aataaggtta
    26521 aacaaataac aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg
    26581 gaaagttgtc tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata
    26641 aattgagaac agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct
    26701 atgacatgag gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa
    26761 gaataaatta gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt
    26821 tgcagaaagc caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa
    26881 agatacacaa acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat
    26941 caaaatcaaa gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc
    27001 aacttataat agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt
    27061 tgttcgaaaa gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt
    27121 ggacatcaat aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca
    27181 caagatcact cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc
    27241 tgttgtcgaa tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga
    27301 gttggcagat gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa
    27361 tgaacggtat attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga
    27421 ctatcagttt tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg
    27481 gtatgatcgg ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc
    27541 agctgcaaaa aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac
    27601 tcatttcgtt cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa
    27661 caaattaaat gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa
    27721 tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca
    27781 cacgatttac agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt
    27841 tgtcttgaaa gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg
    27901 tgcccatcac ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca
    27961 agcgatgaca atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg
    28021 taaaacataa ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt
    28081 gcggaaaata atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag
    28141 atgcaagcgt gtattctttt tttgaacgat aatcacaaaa aaaaatataa tatacatttt
    28201 ttatttaatt ataattaata ttgattgaag tctaggcagt caagtcgaga gtgtaaaata
    28261 ttttgtgtaa atgaaaaaat ccatacaaaa aaggaagtc
//
LOCUS       DAZKOW010000025        28257 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000025 DAZKOW010000000
VERSION     DAZKOW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.025"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..916)
                     /locus_tag="ACG912_001095"
     CDS             complement(20..916)
                     /locus_tag="ACG912_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0512431.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(909..1589)
                     /locus_tag="ACG912_001096"
     CDS             complement(909..1589)
                     /locus_tag="ACG912_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0512432.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1590..2372)
                     /locus_tag="ACG912_001097"
     CDS             complement(1590..2372)
                     /locus_tag="ACG912_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0512433.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2365..3282)
                     /locus_tag="ACG912_001098"
     CDS             complement(2365..3282)
                     /locus_tag="ACG912_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0512434.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3446..3700)
                     /locus_tag="ACG912_001099"
     CDS             complement(3446..3700)
                     /locus_tag="ACG912_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512435.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            4557..7784
                     /gene="fss3"
                     /locus_tag="ACG912_001100"
     CDS             4557..7784
                     /gene="fss3"
                     /locus_tag="ACG912_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0512436.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7897..8211
                     /locus_tag="ACG912_001101"
     CDS             7897..8211
                     /locus_tag="ACG912_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0512437.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8224..8598
                     /locus_tag="ACG912_001102"
     CDS             8224..8598
                     /locus_tag="ACG912_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0512438.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8608..8943
                     /locus_tag="ACG912_001103"
     CDS             8608..8943
                     /locus_tag="ACG912_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFQ0512439.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9026..10366
                     /locus_tag="ACG912_001104"
     CDS             9026..10366
                     /locus_tag="ACG912_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0512440.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10444..11118
                     /locus_tag="ACG912_001105"
     CDS             10444..11118
                     /locus_tag="ACG912_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0512441.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11111..11275
                     /locus_tag="ACG912_001106"
     CDS             11111..11275
                     /locus_tag="ACG912_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512442.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11351..12493
                     /gene="dcm"
                     /locus_tag="ACG912_001107"
                     /pseudo
     CDS             11351..12493
                     /gene="dcm"
                     /locus_tag="ACG912_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12483..12773
                     /locus_tag="ACG912_001108"
     CDS             12483..12773
                     /locus_tag="ACG912_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512443.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13032..14216
                     /gene="mobT"
                     /locus_tag="ACG912_001109"
     CDS             13032..14216
                     /gene="mobT"
                     /locus_tag="ACG912_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0512444.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14213..14350
                     /locus_tag="ACG912_001110"
     CDS             14213..14350
                     /locus_tag="ACG912_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0512445.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            15096..17006
                     /gene="ltrA"
                     /locus_tag="ACG912_001111"
     CDS             15096..17006
                     /gene="ltrA"
                     /locus_tag="ACG912_001111"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0512446.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            17110..17334
                     /locus_tag="ACG912_001112"
     CDS             17110..17334
                     /locus_tag="ACG912_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512447.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            17347..17850
                     /locus_tag="ACG912_001113"
     CDS             17347..17850
                     /locus_tag="ACG912_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0512448.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            17910..18299
                     /locus_tag="ACG912_001114"
     CDS             17910..18299
                     /locus_tag="ACG912_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0512449.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            18286..20733
                     /locus_tag="ACG912_001115"
     CDS             18286..20733
                     /locus_tag="ACG912_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0512450.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            20738..22861
                     /locus_tag="ACG912_001116"
     CDS             20738..22861
                     /locus_tag="ACG912_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFQ0512451.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            22858..23862
                     /locus_tag="ACG912_001117"
     CDS             22858..23862
                     /locus_tag="ACG912_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0512452.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            23881..24792
                     /locus_tag="ACG912_001118"
     CDS             23881..24792
                     /locus_tag="ACG912_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0512453.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            24979..25235
                     /locus_tag="ACG912_001119"
                     /pseudo
     CDS             24979..25235
                     /locus_tag="ACG912_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(25452..25667)
                     /locus_tag="ACG912_001120"
     CDS             complement(25452..25667)
                     /locus_tag="ACG912_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512454.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(25916..26134)
                     /locus_tag="ACG912_001121"
                     /pseudo
     CDS             complement(25916..26134)
                     /locus_tag="ACG912_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(26429..27868)
                     /locus_tag="ACG912_001122"
     CDS             complement(26429..27868)
                     /locus_tag="ACG912_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0512455.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
BASE COUNT         9209 a         5064 c         5915 g         8069 t
ORIGIN      
        1 tctcactaat cttgtataat taaaccctcc gcaacttgac ggagatggag aagatgcccc
       61 cgtctaatct agcggtcatc tctcctccca ttccttccac caattttttt gacaggtaaa
      121 gcccgattcc gctagattcc gtatttgtgc ggctggtatt ttcggtataa aaacgctgga
      181 agactttttc aacagaggtt tgcgttcctg gtttgatagg attggccact gttaatcgaa
      241 tgaatggatc ctcttttgaa aatgtcacct ttgcttgtcc atctgcataa cgcaataaat
      301 tctgtatgat attttgcagc actcgcagca acatgagcgg atctgctaat acagttgtat
      361 ttatcgagtg ttcagcgaac gctacctcga tatttttctc agaaaattgt tcataaaaag
      421 agaaaaatac ttcttccacc tctttagaaa aagaaagcgt atggagatcc atttcttttt
      481 gcttcgtctc tattcgagca agatcataaa actgatccgt catactgagt aatcttgcaa
      541 cagattgttt gatgatcgtt aaatacttct cccgtttctc cgtatcctct acctgttcca
      601 gtaattgtac ataaccgtta atagcagtca aaggagttct gagatcatga gaaaggccga
      661 cgatcgatag tctgatgttt tcttccattt cttgaaatgc ttgtcctttt tgttcatttt
      721 catccaacca tgtattcagt gcatctacta ggtccattag attcttttct cgaaattcga
      781 ctgaaagacg accaccatat tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc
      841 tcttgatttc cctttgcagg aagaaaataa aacttcccat aaagataaca acgagaagca
      901 acagccattt aatcaaatct aaaccccact ccccacaccg tatgaatata cacagcatgt
      961 gcctttgcta gcttccctcg aagattggaa atatgcatat tcactgtttt ctcactatca
     1021 aaatatggtt cttgccaaac tgtttcataa agatttgctc gcgaaaatac ttttttcgga
     1081 tgttctaaaa aaagcttcag cagatcgaat tctcttcctg tcaaatggac agccacacca
     1141 ttcacattta catccctcga ctctttgtcc agtaagattt ccttgtaata gatgcgttgc
     1201 gacagatctt ttacttggac agaagtctga tgacgaagtt ggatgccgat tcgtaacaaa
     1261 agttcttcga tatcaaatgg tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa
     1321 actttatctc tttgatcgct ttttgctgaa agaacgatga tcggaacatt cgatgtctgt
     1381 cggatttctg ctaacaatgc ttcacctgaa agccctggaa gcatgagatc tagaaggatc
     1441 aaatcgacag gatatgtgct taacaataat tttccttccg tacctgaata agcatcaaga
     1501 agtttgtatc tctgttcaag aacctcctcc agtaaggaat gaacagcttc atcgtcttcg
     1561 ataatcaaaa tagtttccag caggaccacc tacttaatgt cagatttttg gaaaataaac
     1621 attcctagag ctgatgatac cacgattgtt actagactga ccagaatcat tttcggtgct
     1681 tgcgaataag ttgcttgttg cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac
     1741 tccgttagaa attccaagcc tttgattttt gcgaaaacac cgggaatgaa ggccgttccc
     1801 atgatataaa gaaaagaaag aagcaaactg acactggtac ttctagtgat gaatgtcaaa
     1861 gccactggaa cagataatgt ccctaatgtc agcagaagca atacaggaaa tacttttaga
     1921 acataatttc catagttttt gattccctca acttcaccaa atccgaaagc aagtgtgtaa
     1981 gcaagcatgg ctagtcccgt gatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg
     2041 agaagtttcg acacataata agtcattcga ttccgtccta aagataacgt attgcgaatc
     2101 gtaccgtttt gaaattcttc cccaatataa aagcacgcaa atgctgcgaa agccagaaga
     2161 atccatgcag ccattttttc taccattaaa atccctgttg caccgttgat gactgtatcc
     2221 gttgcttggt ttaattcatt gccgtatttt gtatgcatat accattcgtt ccaagaataa
     2281 aaaagcggaa gtacgaccat cagtcctagt gagacccaca ttttacggct cttccatact
     2341 tttatttttt cagcagttaa tagattaaac atgtggattg cctccagtta attgtaagaa
     2401 ataatcttcc agtttttgcc caactaggtg aataccatcc acaggaactt ttgcttctac
     2461 caatgcttga ttgatggctg ctacttggtc aattccttca aaaagctgga taacacggct
     2521 agattgtaca aaataatccc tataaccaag agagtcgaga accgttactg cttgttccgt
     2581 cgccgaagtg tcaattttta taaattgacg agattcttgt gccaattctt ctttcgataa
     2641 ttctttgatc aagtgtcctt catgcaaaat accatagtga gtggcgatct gtgacaattc
     2701 atctagcaag tggctagaaa gcaagacggt gatgccccgt tcttttacca aacgttggat
     2761 gatttctcgc atttctgcca tccctgctgg atccatccca ttcgtcggtt catccaaaat
     2821 caaaaattct ggattcgtga tcagtgcatt tgcaatagct aaacgctggc gcatacctaa
     2881 agagaaattc tttgctttct ttttccctgt attttccaat cgcatcattt tcagcaggtc
     2941 ttcgatttct ctatccgaca caccgccgtt cgctgcttgg atacgtaggt tatctcttgc
     3001 agttaattct ggataaagag caggggtttc aatcgattga cccatacgtc gtcttgctgc
     3061 ctgcaaatcc gtcgttccaa ataattcgat cgaaccttca tcaatcgaga tcaaacccat
     3121 aatagttctc ataaaggtag acttacccgc tccattttcc ccaatcaatc catagatcat
     3181 tccacgtttg atttcgattg atactttatc taatgctttg gcagcaccgt attttttggt
     3241 tataccagtt gctttcaaga ccgtttcatt tccaaaatcc atacttatcc ttctttctta
     3301 ttgatgagtg aagtatagtt gacgggtata aagaaagcag ccagaaatgt ataaagaaag
     3361 tgtaaatttt ccaattgaat gtcaaaaaaa acaatcggac aatacgtgtc tttcctatta
     3421 tcaggcagtt tgttatacgc ttgtatcagt ttcctgattc tttcttttcg accaaagtcg
     3481 tttctctgta ttcctcttca ctgaaaattt ttgcttcttt cagatcaagc ttgagtaatt
     3541 ttaccagacc ggttataagg ataactatag caagactccc tcccagaaaa attagactgt
     3601 ctcgcagacg tggttgcgtt gtcttccaat caaagagcgc accaaacagt gctaatccaa
     3661 gtggactggc caccatggta atcgttgtta tcgttgacat aagtcgtcct tgtatatgcg
     3721 ttggaacttc tcgctgaagc cagaccatat aaaaagcaaa agaaaacatt aatccttaaa
     3781 aacggactta tgaaaaataa tacatgccgt ttgggattta caattagcta ccaaataaga
     3841 ggttatgaca aaagactttt cacaatccct tcttccgaat aaacggtgtt ctcctgccaa
     3901 ctcctcggtc acaagtcaca atatttatca aacatgagaa gcaatttttg taaattcctt
     3961 cttatttctt gaagctgacc acttctgtca caacctcaga agcggataaa taatattgtt
     4021 tgatgatact ttatcttttt cccgtttgtt tatagatcga atgatattat agaccaagaa
     4081 tcctacctat taacatagct aaaaacacaa aaaaggcact ttccaaattg tggaaagtgc
     4141 cagaaaagtt aggattaagg taactgcgct cagcattgct tcgcccagta cgattctcgt
     4201 aactcgtgaa ctcgaacgat aatcgcaatt aacgttttga gaactgtgaa gctttacggg
     4261 ctttcttaag acctggtttt ttcttcgtat cattgaataa gtttgaaaaa ccttgattta
     4321 taaaggtttt cccgtaaata tattttatta tatttgatta aatttgaaat gtttgtgttc
     4381 taaaatgtgt tctaatttaa aggcagtata tttcgtattt tagattaaat ttttaggctc
     4441 attcattctc aaatccattc tattcagtca ataaaaattt taaaaagact ttataaacaa
     4501 agatgttcaa gcgagcatct tatttttttg tgcttaattt gaaaggagaa catttcatgt
     4561 ataaaaaaca aaaaaagaaa ttatgtgtat taactatttt gagtttggct gttcaagcaa
     4621 ttgttggagc aattgctcct acgattactt ttgctgatga aattacccat ccacaaacgg
     4681 taaccgtaga actagattta gcacatcaat atgctgttga aggaactttt agtgatggtc
     4741 gtccaatgtc agaagtcact gttccgcatt atgcagtgta caacggcttt aaacaagata
     4801 ttttctgtat tgaaccagga gtcccgattt acaatgagtt cactcctggc tatgagaaaa
     4861 acccgttacc agatatgtcc gataaagcca aattagtttc cgttttatgg aaaaaagtag
     4921 gtacagatgc agatactcat attgtggcac agaagatgat ctggcaagaa gttaatgggt
     4981 atacccttca ctcaataaaa cgatcagatg gtagtgccgt aaatatcgca gccattgagg
     5041 caaaaataaa tcaagccatt gacgattatc agaagaaacc aaccttccat aatagtacgg
     5101 ctaaaacagt attagggcaa tcgactactg tgacggatac gaataatctg aatttgtcag
     5161 agttcgatga ggttgtggaa aatgccgcaa acattgatta tcgtgtgaat ggtaatcagt
     5221 tgataatcac cccaaatgcc aattcaaaag aaagtggtgt gctgacgctt aagaaatctg
     5281 ctggtaccgg aacacctgtc gcctataaaa aggctggcca acagacttta atggccgggg
     5341 cgattgataa acctaatacc tacacaatca aaattgatgt ggaaacggag ggttctttaa
     5401 agatcaaaaa agtcgataaa gaatcgggtg atgttgtacc aggaacggtt ttccatttag
     5461 actttggaaa aactttacct gcaaaagacg tgactactga caaagaaggc attgctacat
     5521 tggatgagat tcctcatggt acaaaagtaa ccattaccga aaaatcagta ccggcgcctt
     5581 atacgattga taccaccccc atgactacca ccattaaagc aggcgaaacc atttacgtga
     5641 cttcaaaaaa cgcacgggaa aaagggcaaa tcattctgga taaaagcgga gttgaaacag
     5701 ggagtgatct ttggaatgac aattattcct tagctggtaa tacgtttgcc attcgcaaag
     5761 acagtccaac cggtgaaatc gttcaagaaa tgaccacgga tgaaaacggt catgcggaaa
     5821 caccaaaaga gattgctaat gcgttggaat tgggaaccta ttatgtgacc gaaactaaag
     5881 ctagtcatgg gttcgtgaat acgttcaaac ctgtaaaagt cgaattgaag tatgccaatc
     5941 aaaccgtagc ccctgttacc agtaacgtaa aagggcaaaa tcaagaagtc actggcgaaa
     6001 ccactttgac gaaagaagac aaagatactg gggataaagc acaaggtaag gcagtctttg
     6061 agggtgctga atacaccttg tttactgcta aggatggaaa agctgttaaa tggagtgagg
     6121 cttttaaacc agagttagcg aagggaacga aaggttctga tgaaacggtc actttgactt
     6181 tagacgaaaa gaaccaagtt gccgttaaac atttagctat caacgagtat tattggcaag
     6241 aaacaaaagc gcccgaagga tatagccttg atgaaacaaa gtatcctgta tccatcaaaa
     6301 aggtggatga tagcgaaaaa aatgccgtga ttacccgaaa tgttacggca aaagaacaag
     6361 tgattcgctt tggctttgat ttctttaaat ttgctggatc agctgcgggc actaccgaaa
     6421 ctggcttcaa tgatctgacc tttaaagtgt cgccattgga agggaccaat gaaatcacag
     6481 gtgccgaaga tgaagcgacc acagcttata acgagcaatt agggtttgat ggttatggca
     6541 agtttgaaaa tcttccttat ggggattatt tacttgaaga agtagaagcc ccagaaggtt
     6601 ttcaaaagat tacgccatta gaaatccgtt ctacttttaa ggaaaacaaa gaagactttg
     6661 tgaagagtga gtatgtcttt accattaccg aacaagacca aaaacaacca attaaaacgg
     6721 tgaccgttcc ttacgagaag ctgacgaaca aagcattttc tgttagtttg aaccgtttaa
     6781 tgctctatga tttgcctgag gaagaagata gtttaacttc tcttgcgact tggaaagacg
     6841 gaaacaaaga attaacttct ctggattcta ccgagctagt tgataaattg agttacaatc
     6901 tacatgaaat caaagaagac tggtatgtgg tagcgcaagc cattgatgtg gatgcgacaa
     6961 aagctgccca agaaaaagat gaaaaagcca aaccagtggt gattgcagaa acaagcgcaa
     7021 cgttggcgaa caaagagaaa acaggaactt ggaaaattca gcataaatta accgccgaac
     7081 aagttttaaa taaaactatt gtcttattca actatgtgta tgaaaataag gaagcttttg
     7141 aagcaggcga taagccagta gcgaaggatg ttagcttgaa taaccaagcc caaactgtca
     7201 gttgtacagt ggaacaccat gtttctatcc aaacaaaggc ccacctagaa aacggttctc
     7261 agacctttac tcatggtgat gtggtggata tgtttgatga tgtgtcgatt actcatgatg
     7321 tactggatgg ctcaaaagaa gctttcgaaa caattctgta tgctttatta ccggatggta
     7381 caaacaaaga aatttggaaa tctggcaaga ttgattatga agtaaatgat aaagaattca
     7441 ccaaaactgt gcttgcgaaa aaagtagata ccggaaaata tccagaagga accaagttta
     7501 cttttgcgga aatcaattac gataaagatg ggaatatcaa tggaaaacac aatgaagatt
     7561 tgaaagaaaa atctcaaacc ttaacaccaa aagaagttcc aaccatacta agtacgccga
     7621 aacaaccgga aacaccaact gttccaagtg gctctcaaga atctagtccc acagtgaaga
     7681 ccttccctca aaccggggag aaaaattcca atgttttact gttcattggt tttactttga
     7741 tttttgcgac ggcgggctat tatttctgga atcgccggaa ctaaggtgat gagatgatac
     7801 aagtaaatcc taagaaccaa cgtgcgcgac cgcctcctaa aaaagtagtc gcgctatttt
     7861 tgtatccaaa actaacaatg aaaagaggaa aagaagatgg aattgaaatt tgtcgtgcca
     7921 gatatggctg aaacctttgg aaagttaagt tatgccggtg aaggagaaat tataacagaa
     7981 ggatatggac ggaatactac ggtgattggt cgcagttatc atttgtattc cagtaagcaa
     8041 cgggccgatg atattgaggt ggtagtagct gcggaagccg gtgaaaagga ttttgatcaa
     8101 gaccaaccgc taaaagccgt gaatccccat ttggttgcca aaggctatga gattgagaat
     8161 cgtgggttta ctgattacgt gttgtatgtt gatgatttag tgaaagcata ggaggaaaag
     8221 aagatgagat taacagaagg tattgtcgta gattcaggtt taacatttgg gaagttgcgt
     8281 ttttccgcat tacgccgaga agtacggaag caaaatgagg atggaacaat tggtaatgag
     8341 gtaaaagaac gaacctataa tttaaaatcc tctgcacaag ggcgaatgat tcaagttagt
     8401 atcccagcta atgtaccctt acgcgagttt gcttatgatg cagaggtaga gttggtcaat
     8461 ccaatcgtgg ataccgttgc caattatgtt tttcgagaag gaactaccgt taattggttc
     8521 attaaggcgg atgatttagt cttgaagcgg cagccgaatc aaggaaatcc tacgaaccaa
     8581 aatgaaagaa agaaataggt gaagattatg gaaattaatg ttgaacatat tttagattgt
     8641 ctcgatcaat atggaaaagg cgagctgact gaggaacaac tcgttaaagc attaacctct
     8701 gatgaaaaaa tgtttctgat tatacatcaa ggtttacttg gagaaaatag cgacagtgaa
     8761 gaagactctg atgtactaga ctggttagga gaagaagagt ctttctttat agttgtcgaa
     8821 gtaaatgaaa acttatgtcg gcaggcagaa tccattttgg aagaaattgg tgtggagatg
     8881 ccggatgcta ttgaaggctt tttaaatcaa ttggttgaaa cgaaacaact cccctcctcg
     8941 tagcagtaaa tgactagcat aactttttga aattgcctaa tgaaatccta ttgggctttt
     9001 tcgattggag gtggaaacca tcaaaatgta caaaggacat cgcatacgcg caggagatca
     9061 gcacttggtt tatcactttg ttcttggctg gctactcgct ctgttcatcg gttggatgag
     9121 cgtcttttat tttcaagagt ttagacaatt tgatatttct aagctatcac tttctactat
     9181 tgaaatcgtt tggtctataa aagatttagt atgtctgctt ggaagtctcg ctttttcagg
     9241 agctatgata ttgctttata ttcacttttt tcttgatcat tggcgaagct tgtggcatcg
     9301 acaaaagcta gctcggatga ttctagagaa ccattggtat gaagtgaaac aaactcaaag
     9361 tgaaggcttt ttcaaagatt tgaatagtag ccgaaccaga gagactatca gttacttccc
     9421 caaaatctgt tatcgaatga aagatggatt actgtctatt cgcgttcaaa tttcacttgg
     9481 gaaataccaa gatcagcttt taaaattgga aaagaagtta gaaagcggac tgtattgtga
     9541 gttagtagaa aaggaactca aagactccta tgtggagtat actttactgt atgatatgat
     9601 cgccaatcga attgggatag acgaagtagt ggcagaaaat gggactctgc gattgatgaa
     9661 aaatcaagta tgggcttatg attctttacc ccatatgtta atcgctggtg gcacgggtgg
     9721 agggaagacc tatttcctcc tcaccattat cgaagcacta ttgaagtcag atgcagaatt
     9781 gtttatcctc gatcccaaaa atgcagattt agctgattta ggtacggtga tgcctcatgt
     9841 ttattcacaa aaggaagaaa tttctgcttg tgtggaagat ttctatgaac gtatgatggc
     9901 tcgtagcaag gcaatgaaag aaatgtctaa ctacaaaaca ggagagaatt atgcctatct
     9961 tggacttcca cccaactttt taatctttga tgaatacgtg gcttatatgg agatgttaac
    10021 gacgaaagaa agtgcggtga ttttgaataa gctcaaacaa atcgtgatgt tgggtcgtca
    10081 atctggtttc tttctgattt tggcttgcca aaggccggat gcgaaatatt taggagacgg
    10141 aatccgcgat caattcaact ttcgcgtggc tttgggtcgt atgagtgaac tcggatattc
    10201 catgatgttt ggggaggtcg aaaaaaactt ctttatgaag cacatcaaag gtcgaggtta
    10261 tgtggataca ggtggcagtg tgattagtga gttttatacg ccacttgtac caaaagggta
    10321 tgatttctta gattcaatta gtaaattaga aaagatgaat gattgagtga tcttcaatac
    10381 tgactttata cttcaagtaa cgtataatac ttttatatag taggaagaaa ggggataatt
    10441 aatatggaaa gtaatgattc ttatttagag tatgcaaaag caaataagga taagtttatt
    10501 tcttatatta tcaaagggaa atcccccgaa gttggacgag acgcagtttt tatggcaggg
    10561 agtcctgggg ctggaaaatc agaggttgcc attggattag ctgagaatta tgataaccat
    10621 gtgattattg atgcggatta ctttcgcacc cagtttcctg attataatgg aaaaaactct
    10681 agtctctttc aaaaagcatc ttcttggttg gtagagcagg catttagata tgtcttagat
    10741 catggctatt cttttattct ggatgcaact tttgcaatat taagtgctga aaaaaatatc
    10801 tcgcgggcgt tgaaaaaaca ttttcgagtg acgattttct atgtttatca agaccctaaa
    10861 atcgcttgga aatttacaca agaacgtgaa attgcagagg gacgtcatgt gccgaaaaag
    10921 gcatttatta acgctttttt caaatcaaga gagaacattg caaaagtaaa gagtaaacat
    10981 ccagatgtat tgcttcatat cataataaaa gattatacga ataatatttc cgaagttcat
    11041 tatgctacgg ataatctaga attattttta ccactagggt atagtaggaa agatttggag
    11101 gttcaattac atgactgaga aagaacaagt aacgaaaatt gtaaagaaat acaataagag
    11161 cattgcggat ttatcggaaa atgctactgc aaaagagttc aaaacggtga ttaaatatgt
    11221 agccgatcag gccaatgaaa aacaacggaa attagttgga ttaaataaaa aatagtagga
    11281 caaattaata atgtatgagc caagagcaaa atgttcttgg cttttttctt tgcaatgaat
    11341 gaggtaatgt atgagatttt tagatttatt cgccggtatt ggtggctttc gtttaggtat
    11401 ggaacaggcg agccatcatt gtatcggttt ttgtgaaatt gataaatttg ctagacgtag
    11461 ttataaagca attcatgata caagtaagga ggtggaaatg catgacatca caagtgtatc
    11521 agatgagttt attcaatccc tcggaccagt ggacatcctt tgcggcggat ttccgtgcca
    11581 agctttttca attgcgggaa agcggcaaag attttccgat actcgaggta ctttattctt
    11641 tgaaatcgct cggttcacca ctcttctccg acctaagctt ttattccttg agaacgtccg
    11701 aggattgctc aatcacgaag gaggggctac gttcgagacg atcctccgaa cgctggatgg
    11761 attggggtat gacgtggagt ggtaggtgct taactcaaag gcctacattc cccaaagccg
    11821 ggagcggata ttccttatcg gacattctcg agacgcatgt accgaacaag tatttcctat
    11881 cattggaccg tcttcaacat ctgatcaaaa catcagaaat ttggtaaata tcaatccttc
    11941 caatcgagga atgggtggac aagtctatgg ttcagatggc gtagcgccaa ccttaacagg
    12001 tgatgaagga atcaaaatcg cactgccagc aagggatgga atctctgtag caggactatt
    12061 accgggaaag tttgaacaag gtaatcgagt ttatgaagtt actggaaccg cccccacctt
    12121 gtcaacgaaa caaggtggta caaaaatcat gattcgaaaa aatgaaacag aatatcaaga
    12181 ggtgaagcca gaagacagtg tgaatctggc ttttcctaat tccacaagtc gccgaggtcg
    12241 tctgggaaaa cagagtgttc ataccttact aacaggagac caacaagcga tcgtcacgga
    12301 tcagtatcaa attcggaagc taacacctag agagtgttgg cgacttcagg gctttcccga
    12361 ttgggctttt gatcgagatt ctcaagtcaa ttcagatagt cagttgtata agcaagccgg
    12421 aaactctgta acagttccgg tcatttttga tattgccaga agattaaaag agttggatgt
    12481 acatggttta tgatgagttg attggagaaa tttattgggt gattggaaag attcagtcag
    12541 acccagaatt agaagaacaa ttgcgacggt taaaatttga tattcgcaag aatggtgtca
    12601 aagtcgctgg cgatccatat ttaatgaatg aggagacgga tgctcgaatt gaaataaatc
    12661 aagtaattgc agaatttgag cggattgcgg accaagcaaa agaaccagat attcgtcaat
    12721 acttgttaga aatgaaagca gagttggaag taaatggtat tacagatgag taacctaaac
    12781 caacacaact gactgttagg cgctgaccgc ggaggattcg cgaagcagaa atcctccgtg
    12841 gtgctgtgcc tgttcatgga cggcgttagc cgaccatgat gtctcacccc cgtatctaac
    12901 aggggggtac aaaaacacaa aaaataagta agcaaccatt aagaatgctt gttgtatcaa
    12961 agatagtaag taatttgtat aggtgttaac tcatttgaaa aagttaacac cttgaatttg
    13021 gaggtgagaa cttggcacaa cggaatttag attatcggct attaaaagac cgacgcaacg
    13081 aatacggcgt ttcacaaaat aaattggcta cggcttgtgg acttagtcgg ccgtacttaa
    13141 atcagataga aaatggtggc gtaaccgcat ctataaaaac catgaggaaa atttttgacc
    13201 aactggaaag ctttaatccc gatttgcctt tgacgttact gtttgactat gtaaggattc
    13261 gctttcccac aacggatgca cgaaaaatta ttcaagaaat tctccatttg aaatttgagt
    13321 atatgctcca tgaagattat gccttttact cctatcaaga acagtatgtc atgggtgata
    13381 ttgtggtgat gttgtctcat gaagaagata aaggtgtgct tttagaatta aaggggcgag
    13441 gttgccgaca atttgaaact tttttactcg ctcaaaagcg tagctggtac gactttttcg
    13501 aggattgtct aaaggccggt ggcgtgatga aacgcttgga cttggcaatc aatgatcgag
    13561 tggggctatt ggatatacct gagctaacga aaaaatgtca gaaggaagaa tgtatctcct
    13621 tgtttcgtac ctttaaaagt tatcgttccg gggaactttt aaaggctgat gaaaaggacg
    13681 gcatgggcaa taccttatat atcggcagtc tcaagagtga ggtttatttc tgtttgtatg
    13741 aaaaagacta tgaacaatat atcaagttag ggattccatt agaccaaact gagacgaaaa
    13801 atcgttttga gattcgactg aaaaatgacc gtgcttttca tgcgattcaa gatttgttaa
    13861 aaggtcgtag cattgagagc accacgtttt ccattatcaa tcgttacttg cgatttgcgg
    13921 ataaagtaga aggtaaaagg cgaaccaact ggcccttgaa tgaacaatgg gggcgtttta
    13981 ttggacggaa tcggaaagag attcaattaa cttccgagcc aaaaccgtat accattgaac
    14041 gaaccttgaa ttggctaggc cgacaagtcg cccctacgtg gaaaatggct aaggagttgg
    14101 atcgattgaa tcggacaacc tatatccagg atatggtaca gaatgcacgc ttatcagacc
    14161 gacataaaaa gattttggag caacaaagta tggcaattga aaatttgatt atatgaaaag
    14221 aggaatcgtc atgggaattg taaaagatat ttttttggta tttggtggca gtatgcttgg
    14281 tgttactgtg atgtgcttga tgaatgcaag tgcagcagcg gatcgagcaa tggaaaagaa
    14341 aggaaagtga caagatgaat ttcggacaaa atttatgtgc gactcgttcc ttagtgaaaa
    14401 gctaaggcac taataaaaga acgaaaaagt ttggttatta ggagaactga taatctgaca
    14461 ggtggaatga tgaagtaacg ctctgaaacg cctgccctga gcctccgaca agcattgtat
    14521 gaggaaactc agaatgtttg aagctcggta aagacggctg aaagataccg taatagttga
    14581 tgaacatata tcttctatcg aaaactatcc agaggtgggt agcgtatcca acaggtcggg
    14641 ggtctataaa atatctatgg ttagaatgtt ctataaggaa ctgacgaaac agcgaatgta
    14701 cgggtctaaa tttgaacttt gtagaaatgc aaaggtacga gaaatcgtag ctggtgtggc
    14761 aaagtaagaa tataggttat gaaatgccat ataacgttac aggcggtatc aagccagcag
    14821 gcttatagct agtgacctaa ggatatatat gtatagataa gattatcgga acgaggaaag
    14881 gtattgggtt ctcatcaaga gaataaactg acgaagaaca ataagcagtt gaactaatgc
    14941 tgaaaagcag agggcgaacc taagaaattc ttgtaatgag aatggaggaa tgccctcaag
    15001 tcgtgtatat attagaaaag tattatgttg tttcaataag gattacgagt aagataaaaa
    15061 agctcactca atttcaagta aaggagtgaa gccttatgcc taaaaataaa aatgaaacat
    15121 tgtgtgtaga agatttaaga cacgctgaat attacgaaat gcaaaacacc tttgatgatt
    15181 tatatgccaa aagtaagaat ggagatattt tcactcatct tatggatatt atcctatcga
    15241 gagaaaatat attactggca tatcgaaata tcaaagcaaa tgcaggcagt aaaacagcag
    15301 gaacagacgg tacaataatt aaagatattg gcaaattgcc agcagaaaca gttgtcaaaa
    15361 aagtaagata cattgttgca ggcagtccac atgggtatcg acctaagcca gtgaggcgaa
    15421 aagaaatacc aaaaccaaat ggtaaaacca gacctctagg tattccttgt atgtgggaca
    15481 ggcttataca acaatgtata aaacaagtat tagaaccaat ctgcgaagct aaatttagtg
    15541 aaaacagcta tggttttaga cctaataggt cagttgaaaa tgcaataaaa gcaacctata
    15601 accgcctaca aatcagccag ctacattatg tgattgaatt tgatattaag ggtttctttg
    15661 acaacgtgaa ccattccaag ctgataaaac agatatgggc tatgggaata cgggacaagc
    15721 acttgatatt cattttaaaa aggatattaa aagctccaat caaaatgact aacggtacaa
    15781 taacatatcc agagaaagga actccgcaag ggggaataat atccccacta ctagcaaaca
    15841 ttgttcttaa tgagttagac cattgggtag aaagccaatg gcaggaaaat cctgttacaa
    15901 aaaactatgt ggttcatatc aataaaagcg gtagcccatg taaaagcaac gcttacaaag
    15961 aaatgaagaa aacaaagtta aaggaaatgt atatggttcg ttatgcagat gattttagag
    16021 ttttctgccg ctacaaggaa agtgctgaaa aagccaaaat agcaatcact caatggattg
    16081 aacaacgatt aaaattggaa gtatcacaag aaaaaacaag aattgtaaat gttagaaaaa
    16141 gatactccga ctttcttggt ttcaagatta aaatgatacc gagaagaaag aagttagttg
    16201 ttaaatcaca tataagtgat aaacaattca aaaaccaaaa agacaagctg gtatcacagg
    16261 caaagaaaat tgcttctccc tcaaaaggga aaacggaatt agaagaaaca agactttata
    16321 attcaatggt attaggtatg caaaattatt atcagattgc aacgaatgta aatctggatt
    16381 gtatgaaact gaatagagct gtaatggcta tcttcactaa cagattaggt acaaacaaaa
    16441 ggggcagatt gagaaaaaca ggcagacaac ttacgacaaa ggaggcaaca agatatggta
    16501 aatctaaaat gcttcggtat gttgctggcg agggcgaacc tattttccct gttggatata
    16561 tccaacacag aaaaccaatg gcaaaggtgt atttagcgaa ttgctacact ctagagggaa
    16621 gaaaatttat ccacaccaat ctaagtgttg ataaattact gatgtaccag ttaatgaaat
    16681 tatctttgga aaatcgcaca atagaatacg cagacaatcg aatttcgcta ttttctgctc
    16741 aacatggaaa atgtgctata actctggaag aatttcaaca agctaatgaa atacattgtc
    16801 atcatattgt accaactgga gctggtggaa atgacgatta taaaaatctt attttggtaa
    16861 aagaggcggt acaccgtcta atacacgcta aaacagaaga aacaatacaa aaatatatag
    16921 ctctattgaa gctaaataaa gtccagctca taagactaaa taaatataga gtattagctg
    16981 gcaatcaaga attaaacttg atataggtct actacataaa tacttttcaa tataaacacg
    17041 atggaacgcg gagtgcggtg aaagtcgcac gctccgtgtg aatgggggga aaagtttgag
    17101 atatttataa tatcaggaac ttacctatcc atatatcaat ggtttttaac caatgcccaa
    17161 tccttagtct tactggcaat tgtggtcatt ggtttgtttt taggatttaa gcgggagttt
    17221 tccaaactga ttggcttttt ggtgattgct ttgattgcgg tcggattggt ctttaatgct
    17281 tctggggtga aggatgtctt actcagtttg tttaaccgaa ttgtcggtgc gtaaggggga
    17341 agcacgatgg aacagatgcg tgtttatatt gcgaacctcg ggaaatataa cgaaggtgaa
    17401 ttggtggggg cgtggtttac cccacctgtc gattttgatg aagtgaaaga acgaattggg
    17461 ttaaatgacg aatacgagga atatgcaatt catgattatg aacttccctt tgagattgat
    17521 gagtatacgc caattgagga aattaatcgg ttgtgtggct tagcggagga acttgaagga
    17581 acgccagttg gtgaagttgc ttctgagatt caacatgctt tcttcaattc ttttgaggag
    17641 atggtagagc atgtggatga tattatctat tatccagatt gcgatgatat gagtgatgta
    17701 gcatatttct tgattgtaga agctggtgat tttggagaag taccagaaca cttgaagacg
    17761 tatattgatt ttgatgcgtg tggtagagac ttggagattg gaggcaatta tttggtaacg
    17821 agtcgaggtg tttttgagta tccatattaa caaaattatt gaagggtagt tgatttctaa
    17881 actatccttc tttttaaagg aatgatttga tgaagaaaat aaaaagctac accagtattt
    17941 gggcagttga aaaggtcatt tatgcaatta atgactttca actgcctttt cctgtgactt
    18001 ttaaccagat ggcttggttt gttttatcct tgctgtttgt catcgtattt gcccatgtgc
    18061 ctccactatc tatgatagag ggggcctttt taaaatactt aggtataccg gtggcagtta
    18121 cttggttcat gtcgcaaaaa acgtttgacg gtaagaaacc attgggattt ttacgatctt
    18181 ttattagcta tcacttacga tttaaggtca ctttcgcagg aaagaaagtc aaagaacaga
    18241 agaaacgatg ggacgaaccc attactctgg taaggagtgt gaactatgta cccgattaag
    18301 tatattgaaa ataatcttgt ctttaatcaa gagggagaat gttttgccta ctatgagtta
    18361 ataccttaca attactcctt tttgtcacct gaacagaaat atcaagtcca cgataacttt
    18421 cgtcaattga ttgcgcaaaa tcgtgaaggc aagattcatg ccttacaaat tgccacagaa
    18481 agtagtattc gtgccacgca agaacgatca aaaaaggaaa ttaccgggcg attaacagaa
    18541 gtagcgaaac aacgaattga tttacagaca gaggcattag tatccatgat tggcgatagt
    18601 cagattgatt atcgcttttt tattggcttc aaactgattg cgacagatga agaggtcaat
    18661 ctaaagaatc tcaagaaatc tttcttctcc ggctttcaag aatttgtcta tggagtgaat
    18721 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgccg atataccaag
    18781 cttgaaaaac taatggaaag taaattggct cgaagattta aagtcagacg agtcacaccg
    18841 agtgatctga tgtatttgat tgaacatatc tatggcgaaa agggtacacc atttgaagaa
    18901 tatgaatttc agctaccaaa gaaaaagctg aagtcggaaa ctttggtgaa acgttacgac
    18961 ttattacgtc ccagtcgttg tttgattgaa gaaaagcccc ggtacttatg catggaacat
    19021 gagaatcatg aatcatatgt agcgtactta acaattaata caattgtggg ggaaatggag
    19081 tttccctcct cagaactttt ctattaccag caacaacaat ttacattccc aattgacact
    19141 agtatgaatg tggagattgt gacgaacaaa aaggcacttg ctaccgttcg caataagaaa
    19201 aaggaattaa aagacttgga caaccatgcc tatcaatctg acaatgaaac aaattccaat
    19261 gtcttagatg cattggattc cgtagatgaa ttggaaacta ccttggatca gtccaaagaa
    19321 tccatgtaca aactgagcta tgtggttcga gtaagtgccg aatcagtaga tgaactaaag
    19381 cgccggtgtg atgaagtgct agatttttat gatgatacca atgtaaaact agtccggcct
    19441 tttggcgata tgatggggct acatgaagaa ttcttacctt ctagtaagcg atacatgaat
    19501 gattatatcc agtatgttac gtctgatttt ctcgccggac ttggttttgg cgccactcag
    19561 atgttagggg agttggaagg catttacttt ggttataacg tagatacggg gcgcaatgtt
    19621 tacctgaaac cggcattggc ctcacaaggg gtcaaggatt cagttaccaa tgcgctagca
    19681 gcggcattcc tcggttcact cggtggcgga aagtccttta gtaacaatct cttagtttat
    19741 tacgccgtcc tttttggtgg acaagctgtg attgtcgatc caaaagggga acggggtggc
    19801 tggaaagaaa ccttgccaga aattgcggat gaaatcaata ttctcaactt aactagtaag
    19861 ccaaaaaatc aaggattact tgatccttat gtgattatgg agaaaaagaa ggattctgaa
    19921 agtctagcaa ttgatattct gaccttttta acaggaattt ctagtcgaga tggggagaag
    19981 tttccagtgt tgcgtaaggc cattcggacg gttacccaaa gcaatcaacg gggattatta
    20041 caagtaatta atgagcttcg aaaagaaggc tcaccagttg cggaaaatat cgcggatcac
    20101 atcgagtcct ttaccgatta tgattttgcc catttgcttt tctcagatgg ttcgattacc
    20161 cagtcgatta gtttagatag gcagttaaat atcattcaag tagcggattt ggttttacca
    20221 gatgcagaaa ccacttttga agaatatacc accatggaat tgcttagtgt ttccatgcta
    20281 atcgtcattt ccacttttgc cttggacttt atccatagta atcgagaaat cttcaaaatt
    20341 gtagacttag atgaggcgtg gagttttctt caagtagcgc aagggaaaac attatcgaat
    20401 aagctcgttc gtgctgggcg ggctatgaat gccggggttt actttgtcac gcaaaatagt
    20461 gatgatttac tggatgaacg cttgaagaat aatatcgggt tgaagtttgc gtttcgcagt
    20521 accgatattc atgaaatcaa gaaaaccttg gaattctttg gcttggatcg agaagatgaa
    20581 agcaatcaaa aacgcctacg agatttggaa aatggccaat gcttgatgca ggatttgtat
    20641 ggtcgggtag gcgtggttca ggtacatcca gtctttgaag aactcttcca tgcctttgat
    20701 acccgtccac ctgttcaaga gagaatggag tgaggggatg aaaaagaaac tttttcggat
    20761 tgtgggaatc gtttttatta gcatactcac tctgatagtt ctattgagtt tagttggtac
    20821 ggtagccgag gcgacagggc tagttgatga tacagttgaa gcaggaaatc tatattccaa
    20881 atattcactg aacaactatc aactagattt ctttgtggat agctcttggg attggttgcc
    20941 ttggaattgg ggtgatggct tagggaaaag cgtgatgtat ggtctttatg ccattactaa
    21001 tttcatttgg acggtgagtt tgtatttgtc gaacgccacg ggttatgtgg ttcaagaggc
    21061 ttacaaatta gatttcattt cggatacggc tgaaagtatt ggaaagaata tccaaacatt
    21121 ggctggtatt actgaaaatg gactccagac ctccggattt tattttgggt ttctattatt
    21181 gatgatttta gctctggggg tgtatgtggc gtataccgga ttattaaaac gagaaaccac
    21241 gaaagcagtt cgagccgtga ttaattttgt gatgattttt ctactatcgg gttcgttcat
    21301 tgcttatgcg cctacctata tcacaaaaat caatgacttt agctcagatg ttagtgaagc
    21361 ggcccttacc cttggaaccg agatcgttgt acccaattca gaaagccaag gaaaagatag
    21421 tgtggatttg attcgggata gtttgttttc gattcaagtc caacaacctt ggttactact
    21481 gcaatttgat gattccaaca tagaagaaat tggtgaagac cgggtcaaca agattctatc
    21541 tgtgagtcca gatgaaaaca aaggcaaaga tcgggaagaa gcggtcaagg cagagattga
    21601 agacaatgac aatgcgaatc tcagtattac caagaccatg aaccgtttag ggaccgtggt
    21661 ctttttggta ttgttcaata ttggcatttc cttctttgtt tttcttctaa cagggattat
    21721 gttgttctcg caaattctct tcattatctt tgcgatgttt ctgccaatta gtttcttatt
    21781 gtccatgttg ccgacttatg aaagtttagg aaagaaggcg attattcgtt tattcaatac
    21841 gattatgatg cgagcgggtg ttactttggt gattacgact gcatttagca tctccacgat
    21901 gtttttcaat atttcggcaa cctatccatt tttcatggtt gcttttctgc aaattgtgac
    21961 ctttgccggt atctatttca aattgggcga cattatgagt atgttcaacc tccagagtaa
    22021 tgatagccag tcaatgggaa gacgcgtcat gcgaaaaccg caaatgttaa tgaatcgaaa
    22081 attgcggcaa ctcaatcgaa atgtgggacg agctttggct tttggtggtg gtgcagcagt
    22141 cggaaataag ttggcaaagg aacaatcaaa atccaaattt aagccatccg gttcttctct
    22201 acgaaagaat tcacgattac cgaacgatca cgaaatacta tctgacttaa ctaaggagaa
    22261 tccagtttca aataataaga aacaatcccg aatgaatttg gctgggagaa aaatcggcaa
    22321 agttttggat acccaagctt tggtgaaaga taagactaaa caagtaaagg atcaggtaag
    22381 gaatacgcca acgaatttga aatacaatct ccataaagga atcgaaaagt cgaaaaaagc
    22441 accagaagaa ttcaagcgcg gactgttcca agaaaaagcg gatcgagtgg atttgcgaga
    22501 aaaacaacga caacgaagaa atgcgaaaat ggacgaaaaa cgcaagatac tgagtgaagc
    22561 aacaaatcgt cagggaaaaa gaagggcgaa tattccaata gataaagcag ctcaacctca
    22621 gaaagatatg atgctgaaaa aagaagcacc aaagcggatt gtgagagcaa atctgaatcc
    22681 cgaaatcaaa cgaaaactaa ctagtcaaga aatcatttca aaagggaaaa gccaatcagc
    22741 aagtaagaaa aattctttcc aacaagtgaa acagaggtcc actctgccaa agcagaccaa
    22801 tcaaaaagta caaaaaagaa tgaaccatcg tccaaaatca aggtcaggtg agaaaaaatg
    22861 aacttgaaga aagtgggtgt tatttcatta acttttctga ccatctcttt tatgggtatt
    22921 ttgatgtgtg tgggtttgtt gtttggtgaa gacagtgagg gaaatggtag ttctggcact
    22981 tctcctggcg gaactagtgt ctcagaagaa gtattgaaac accgtgcaat ggtggaaaag
    23041 tatgccaaag aatcggatat aacagagtat attccaattc ttttggcgat tattcaagtg
    23101 gaatctggtg gcacaatgga agacgttatg caaagttctg agtcagccgg attaccacct
    23161 aattcattaa gtaccgaggc atccatcaaa caaggctgtc tctattttgc gtctttggtt
    23221 aagcgttcca aagaactagg gtgtgatcaa gatagtatta ttcaggccta taactatggt
    23281 ggcgggtatc tagatcatgt cgggaaaaac gggaagaagc atagttttgc tttagctgaa
    23341 tctttttcaa aggaaaaatc aggtggtcaa aaagtcgatt accccaaccc aatcgcgatt
    23401 aaggaaaatg gtgggtggcg gtataattat ggcaatatgt tttactgttt attagtgaag
    23461 caatacctaa ctacaactca gtttgatgac aaaatagtcc aagaaatttt tgatgaggca
    23521 tttaagtatg aaggaacggc ctatgtgttt ggtggctcaa gtccagaaac ggggtttgat
    23581 tgtagtggct tgacccaatg gtgttatgcc aaagttgacc tgaaacttcc tcgggtggct
    23641 caggatcaat atgatgccat gactcatatt gatctaaaag atgccaaacc aggtgattta
    23701 gttttctttc attcaaccta tgatgccggt acttatgtga cccatgtggg catttacgca
    23761 ggggagaatc gcatgtttca tgcaggagat ccagtggggt ggacgaatct aacggaaaac
    23821 tattggcggc agcatcttat cggagctggc cgatacaaat aagagaaaag aggaaaaaga
    23881 atgaagataa aaattgaacg gaatcaaaag gaaaagagcc caaaaaagaa aaaagaacga
    23941 gttgtatccg tgggtaagca tcgaaaaatg gtgcttgccc tttggctttt attgtcttgt
    24001 agtcttgctt ttggcgttta taaaaatttc accgccattg atcaacatac aactcatgaa
    24061 aaagtggtta taaaagagaa ggtagtgaat acttctggcg ttgaaagttt tacagaggat
    24121 tttgcgaaag aatatttttc atggaaaaat aacaaagaag caattgaaaa gagaatgagt
    24181 aatctcgaac agtaccttac agaaaacggt ctcgctttaa tccaagatat ggttcgagca
    24241 gatattccca cgagttccga agttcaatct gtaaaaattc tagatgttga aaaacactcg
    24301 gaagaatttg tcgtttcatt tttagtggag caacatatta cagagggaaa aaagacgcaa
    24361 tcaatttctt ctgcatatcg agtgacaatc tttgaagatg aaaacaggaa tcatttagta
    24421 actagtttgc caacaatgat tggaaagcca gacaaggcaa aatatgaagc aaaacaagta
    24481 gaaaacgatt cagaaattga tgccaaaaca acggaagaaa tcaccgattt tttagaaaca
    24541 ttttttaaac tttatccatc agcttctgaa aaagaattgg agtattatgt tgaaaatggt
    24601 gcgatgcgac caatcaatat tgacttaaag ttttcggagt tagagaatcc agtttatcta
    24661 caaaacggaa aaaattttct agtgagtaca aagataaaat accttgataa tgtatcaaaa
    24721 accactagct actttcagta caaattagta ttacaaaaaa atggaaactg gaaaatcgtt
    24781 gatgttaaat aataatgact caatctaatt ttattagaag ttataagtag tgtaacttat
    24841 ttcaggcaga agtcgcccat cctctatagg tgggtgatga atgccattcg gtaactcgaa
    24901 aaccgacaat taagacatag tgccttttta ttttaatcca tgtcataatc agtctatata
    24961 ggaaatggtt gatataccat ggaattagat acaaataatc attcagtttt tcttcttggt
    25021 taccccctta ttttagtagt gaaacattgt aaacatgtca ttgatgatgt aatgtcagct
    25081 tatgcaaaaa ctgcctttga aagaatttca gaatcacatc acattacctt agatgaatga
    25141 aatcgtgata aagaatactt tcatattatg ctcaaagcac aacctaaaac cgaattaaca
    25201 aaattcagca atgcctaagc tcacgtctga taaaatgaga ctttctaagg gtcaaaaatt
    25261 tcctttgaat acgattattg cttagataaa agtaaaagcc ttagaactac ggggatagct
    25321 tggttaataa gagatatcgt tgttagtaaa gacataggct tgcaagtatg ctctattccc
    25381 aagaatctcc cccttcaagc accaaggatc gttaaattca gctaagtggc aagtaattca
    25441 ctagacgata attatctcca tatttcaatc tcagcttttc catttttcat agcaaattcc
    25501 gttattttta tagcatctaa aagattcagc ttttccactg atctttcacc acttttcagc
    25561 ttttttattg tgcttaaatt gataccagta ccttttgata ttgaattgga agtaaaatca
    25621 ttaagggttt tttttattaa attcatatcc gcaattccag cgttcataag aatgaagatt
    25681 ctcctctcat agactacttt gaaaatggta ctacagtggc tgtttatata tttcagtagg
    25741 ttactgtagt ggtcatctta ttataattag aaaaggaacc taaatcaaaa atgctaaaga
    25801 aaattttcat agtattaagc ctctcatgac taaagccacg gaattcttga taaaagagta
    25861 tacttgcaag cctaaacagc ggtatctctt attaaccaag ctatccccgt tgttcctaag
    25921 gttcttgatg ttttctaagg caatgcttgt attcatagcc cttggtcaaa atattgatac
    25981 tatcgttgac gtttcgatta tgatgtatac ggcaaatagg acaagtccat tctcgaattg
    26041 tgagtgactt cttaccatct gtgtgtctac attctgaacc aatttgacta gacggaaatc
    26101 atttatctat cttaatgatt ttttgacaga agtatcgaaa atctttacat aatctgggtg
    26161 atgtattctt atgcagatga gagcgttggc tccgtattca taaccagtag cctctaaaac
    26221 gtagtccatc ggtgggatac ctagcttgcg gttgccagcg gctttcgcag tcttcttgtg
    26281 atcaaatcaa gcatctcatt gagcactagt attttgaaga taatacttga gtttttcggc
    26341 ttttagaagt tcacttcatc ttccatcatc catggaaatc ccactcaccc atacgcaggc
    26401 ctccaaaaat tcttcaacag tggttttttc agacattttt ctgaatacct ccttgttttt
    26461 tgatacgcca gatagtcagg aaaaaggcaa caaaacacaa aacaaagctg cctaaaaagg
    26521 taatcttcat tccatataaa aagacatccg gccgatttgc gatatagtta gttacttgct
    26581 ggcccatctt agcagacatc gcttgatata agaccgtcgt agaaagcgaa atcccgctga
    26641 ccatccccaa atttcgggca aaggaattca tcgagcctgc cagtcccaag ttttcgatag
    26701 tgaccgagct cataaccatc gtattattgg gggactggaa caaggcattg ccggcaccca
    26761 tgatggcagt agcgacaatg tatagccaca aaggagagtc cactgacaaa aacatataca
    26821 tgacctgggt taccgacaat aagcccaagc ctactaatac caaaatctcc gggccaaccc
    26881 ggtcggtcag gtagccgctt aaaggcgccc cgacgaccat catcaaagga aagaccatca
    26941 tcaacagccc agcatagcta ggactcaacc cgcgagcatt ttgcagataa aaaggaatca
    27001 ctacattgac aaagaagttg gaagaaaaaa tcaacaaggc tgtaatcaag ctcatggtaa
    27061 aaatcttgtt cttaaaaata ccgaaagaaa tcaatggaga ggctgctttc ttttcaactt
    27121 ttaaaaagct gacaaaggca atcactgaaa ttacgaacag tacaaggggg actggatgga
    27181 aaaagccact ttcttgcccc aaaaagattg ctccaaaaaa gctgataatc gtagccgcaa
    27241 tggcagcaaa gccccctcca tcgattgtat ggccgctcat ggtgatatct tttggtaaga
    27301 atttttcagc aatcaacatc gttacgattc ccaccggtac attgatccaa aaaatgtacg
    27361 cccaagaaaa ttgggacaaa atcaagccgc caagacctgg tccagcaatc gagcctaatg
    27421 aaacaaacgc acctattgct cccaatgccc gaccacgttc atgtatggga aagacttccg
    27481 tgatgattcc ggcatttgta gccatagtca tactagcacc aatggcttgt accacccgtg
    27541 cgaacaataa aatacctagc gaatgattga acccacacaa cagcgatccc aagacaaaga
    27601 tcagcgtacc aatccgaaat actttgattt tcccacggct atcgccaatt ttaccaaaca
    27661 gcaacaaaca agaacagaca atcatcagat aaacggacac tacccattcc gcttgattca
    27721 ttggtatgcc catatcatct gagatggtag gtagtgcaat attgacaata ctgccatcca
    27781 atgtggacat gaaggtaaac atagatatag caatcaaaat ccaccagcga tttttttgga
    27841 cttttgcatc ttcttgataa ctatacataa taggatttcc tttctaatga tcgtgtagct
    27901 aagaacaata taacctatgt ctactgccca acaacttctc gcgactctag ccataggaag
    27961 cttgagtaat ttcgatttga tatcctcatg taaccatgta agttggagat gctttatttg
    28021 aacttactct atagtaacta gtgataattc gctttacttt cttctagcaa tttctaaaag
    28081 tcgacttcat tgttcttcag attaatagtg tgagctatct cgtgagacgt aagcaaattt
    28141 ccattctact ttttcccaat tttttcatat ttatggtgtt gcacctattt gttttccaat
    28201 ttgcttttca aatacattgc tatgatattc aattccatag gattatacta aatatca
//
LOCUS       DAZKOW010000026        27696 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000026 DAZKOW010000000
VERSION     DAZKOW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.026"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(143..670)
                     /locus_tag="ACG912_001123"
     CDS             complement(143..670)
                     /locus_tag="ACG912_001123"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0512456.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(737..2239)
                     /gene="eat(A)"
                     /locus_tag="ACG912_001124"
     CDS             complement(737..2239)
                     /gene="eat(A)"
                     /locus_tag="ACG912_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0512457.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2497..3618)
                     /locus_tag="ACG912_001125"
     CDS             complement(2497..3618)
                     /locus_tag="ACG912_001125"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0512458.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3744..4685
                     /locus_tag="ACG912_001126"
     CDS             3744..4685
                     /locus_tag="ACG912_001126"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFQ0512459.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4835..5731)
                     /locus_tag="ACG912_001127"
     CDS             complement(4835..5731)
                     /locus_tag="ACG912_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFQ0512460.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            6000..7340
                     /locus_tag="ACG912_001128"
     CDS             6000..7340
                     /locus_tag="ACG912_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0512461.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7558..8001
                     /locus_tag="ACG912_001129"
     CDS             7558..8001
                     /locus_tag="ACG912_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFQ0512462.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8505..9923)
                     /gene="nhaC"
                     /locus_tag="ACG912_001130"
     CDS             complement(8505..9923)
                     /gene="nhaC"
                     /locus_tag="ACG912_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFQ0512463.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10326..11549)
                     /locus_tag="ACG912_001131"
     CDS             complement(10326..11549)
                     /locus_tag="ACG912_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0512464.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11580..12419)
                     /locus_tag="ACG912_001132"
     CDS             complement(11580..12419)
                     /locus_tag="ACG912_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0512465.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12436..13116)
                     /locus_tag="ACG912_001133"
     CDS             complement(12436..13116)
                     /locus_tag="ACG912_001133"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0512466.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13118..14191)
                     /locus_tag="ACG912_001134"
     CDS             complement(13118..14191)
                     /locus_tag="ACG912_001134"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512467.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14247..14485)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14528..14761)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14838..15509)
                     /locus_tag="ACG912_001135"
     CDS             complement(14838..15509)
                     /locus_tag="ACG912_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0512468.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15596..18244)
                     /gene="mgtA"
                     /locus_tag="ACG912_001136"
     CDS             complement(15596..18244)
                     /gene="mgtA"
                     /locus_tag="ACG912_001136"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFQ0512469.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(18586..18760)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            18933..19370
                     /gene="ndk"
                     /locus_tag="ACG912_001137"
     CDS             18933..19370
                     /gene="ndk"
                     /locus_tag="ACG912_001137"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFQ0512470.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19392..20258)
                     /locus_tag="ACG912_001138"
     CDS             complement(19392..20258)
                     /locus_tag="ACG912_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0512471.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20418..22511)
                     /locus_tag="ACG912_001139"
     CDS             complement(20418..22511)
                     /locus_tag="ACG912_001139"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0512472.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23260..23883)
                     /locus_tag="ACG912_001140"
     CDS             complement(23260..23883)
                     /locus_tag="ACG912_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512473.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(24042..25439)
                     /locus_tag="ACG912_001141"
     CDS             complement(24042..25439)
                     /locus_tag="ACG912_001141"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0512474.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25471..26442)
                     /locus_tag="ACG912_001142"
     CDS             complement(25471..26442)
                     /locus_tag="ACG912_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0512475.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26816..27241
                     /locus_tag="ACG912_001143"
     CDS             26816..27241
                     /locus_tag="ACG912_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512476.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         7979 a         5731 c         4814 g         9172 t
ORIGIN      
        1 tcaatacttt tattaaaata attaaatttc atttctaaag gttgagttta tattatcaaa
       61 agaattgtca taacctaaaa aaggcactgc taagcaaaag cagtgccttg aaatatttta
      121 tttaaataac acaaaaacat aattactcat aagaaaccaa ttctgtatta tctaccatga
      181 atgtaaagaa cccctcatta ggcatcggat gaaaataagc attgatcact cttatcaagg
      241 cattatggca aactaacaga tagacatttt cttcgtcttc gatgcattct ttcaataaag
      301 gaacgatccg tgcatacaca tctaagactg actccccatt tgggaatcgt acagcgaacg
      361 ctaaacgtgc tttttgaaag ttctcatctt tgcttggcag tccatcataa tccccaaaat
      421 ccatttctac caatcgttca tcaacggtca gcggcaagct gagacgatca gcaactgctt
      481 gtgctgtatc tcttgctcgt tgaagagggg aatggatgat tttcgtgatt ggaacaggca
      541 aatctactac cttttctgct agtttctgtg cctgcatctg tcctttttca gtcagcggca
      601 catctgcccg tccgcaaact aatccttgtg cgttccaagt tgtttctccg tgtctcgtta
      661 cataaagcat gtgattctct ccttcgttgg caaagttccc cttaaccata ccttgttgtt
      721 cttgagatta caagggttag attctctcaa gagaaataat ctcagtagat actttatcca
      781 ggaaggtttg atcatgttcc actagtaaca tggcaggttt cacgttcaag atcagttgtt
      841 ctaattgttc ttgattgaag acatccaaat aattcaatgg ttcatcccat gtatatagtt
      901 cagctggctg tgataaagat ttagccaatt ccactttttt ccgttggccc atactcatct
      961 gctcgatctt gttatgaaaa acatctcttt ccatcccaag ctttcggagg ttgttcaaaa
     1021 atgcttggta gtctacttgg tttttctctg caaattccgc caacgttcct cgattgtctt
     1081 cataattttg gcttgcataa ctaatgctca gatgttttgg ctggtatttt tctcctataa
     1141 ccttgccgtt gaatgccccc agaagataat ggataatgga tgactttcct gcaccgttag
     1201 gacctgaaat cgccacccgc tgatgaggct cgattgtaaa atgaattggc tcgaataaca
     1261 gattttcata ccctaattga agatcttcta cgcttaaaag tcgcttatgg tgagacgctt
     1321 ggctattcat cgtcaaagaa tcgatatatt cgatatcttt taatagtttt tctttctcgc
     1381 tgatctgggt ctccatccga ttcacgattg ctttggatcg tttcatcgtc cgtgcagcat
     1441 cagcacccac tgctccttta ttcactcgtc tagattcagt atcgatgaat ccgacttgtt
     1501 tctttgtttt atctccttct cgggaacgag accattcggc tttttcagct gctgttttct
     1561 ttagcctact gacttctttt ttcaattttt cattttgagc catttcaaat tcatcacgaa
     1621 gttttttctg ttcttcatag atagagaaat tcccttgata aagttccagt tgactttttt
     1681 cgattgctaa aacatggtcc actacttcat cgataaatcc ccggtcatgg ctgaccacga
     1741 tgaagccttg ttttttcttt ttcaaataag ccgctacttg ttttctacca gagatatcca
     1801 aatgattcgt tggttcatcg attaacggga aatgagtgtc atccacaaat aaaagtgcca
     1861 gtaggacttt tgtcttctct ccccccgata gtgtgctgaa ttctctccat aagatttcag
     1921 gatctgtttg catcaattgg agctctcttt cgatttccca tatctcaaaa tccgtaatat
     1981 catttaacac gtaataggtt aaacgttctt tatcttttgt ctgttgcggg aaataggcaa
     2041 attcttgctg atggattact tgcccttgat aaggtagttt gttttgtaga atattcagta
     2101 aagttgtctt tcctcgaccg tttcgtccga taagtcctag tttccattgt gtgtcaaaat
     2161 ttagatttgc ttgttcaaat aataatgtgc cttggctgtc gtagccgaat gtcagatttt
     2221 ttatttcgat tttagacatt ttccatcacc tcgtaaaaaa agaccagtcc tattttcaga
     2281 actggctaaa gaggatgcaa gggttggata cacacaaaaa gacccataca tcgaaaaaac
     2341 caagccaatc ccgaaaatct gcacactgct tccttgtctc cacaaggtac agtctgaatg
     2401 cagctttcat caatcgactt aatttttcaa tgcacggatc cctcactttg tcctatattt
     2461 gaaattacga tagcataaag aaaatgattt tgtcaactac ttcgggagga tggtgaaaaa
     2521 tctgctaagt gtctgtccta agatttttgt tttttctaat gtttgcctgt gttccattgc
     2581 ttcttctaaa cctattggac ccaaatgaat acttaagaca attgggagaa aatcagaaag
     2641 tccttctagc tcttggtctc tacttccgca aatagcgatt attggttttc gatatttttt
     2701 tgcaagcact gctacaccgt atggaacttt tccgtggatc gtttgctgat cgatccgccc
     2761 ttctcctgta atgatgacat ctgcttgttc gatctttttt tccaatccag tgatttggct
     2821 gactaaatcg aaaccagaaa ccatcttcgc tcccaacaaa aaaagggctc caccaatacc
     2881 gcctgctgct ccacttcctg gaatatcatc taaatccacc tcccagaaat cttttatttg
     2941 ctgagcaatc tttcctgctt gtttatctaa gaaagctagc gtcgtatcta ttcctccttt
     3001 ttgtcttcca aattgccaag cagctccttg ttgacctgta tatggattgg taacatctgc
     3061 agcgataatc aattttactt tagaaacaag aacgtttctt cgatcaaaat gaagtttttt
     3121 agtagataat aaaggatttc cttctgtgta cccttctaaa gaagcaccca aggattggag
     3181 taatcctaga ccaccatcgc tacacccgct tcctcctaaa gtgacaataa tttgttctac
     3241 ttcttgaagc agtgcatctt taataagttc tcctaaggca taactggaag caagtcggat
     3301 agtttgatcc gttggtttat caagtagatc taaccccaat gcttgtgcag attcgatgac
     3361 tgcggtgttt tttccttgcc aagttgtcaa taaatagctg atttttcgct gacggaataa
     3421 gagatccatc ccatcaatgg tacgccatgt tcccctgagc gcgtaataga ttgcttctgc
     3481 agtattttct ccgccatctg caatcggcac attgattttt cttcctttac gatcttccca
     3541 gccatttaga actgcctgat ttagttcaaa tgaagaagca cttcctttaa atgaatcaat
     3601 tgccgccaaa atatccatct gtatccctca ctttcttctt aagtaagaca ctttttttca
     3661 tttttttcaa acaaaacaat gctattttct tccatttttc tatatactaa aagggaactc
     3721 attgaataaa ggagagaaaa aacatgaaaa tcgcaatagc tggggccgga gcaatgggca
     3781 gccgaatcgg attgatgcta catcaaagcg gaaatgaggt cctcttgatc gatcgttggc
     3841 ctgctcacat tgaagccatt cgtacaaatg ggttgattgc tgactttaac ggtaaagaag
     3901 tggtcgcaaa actgccaatc tattcaccag aagaaatcat cgaaagcaac gaacacgtgg
     3961 acttgatcgt agcattaaca aaagccaatc aattagatga tatgttttgt tcgatccaat
     4021 ccattattac tgacaatact tatgttttgt gtttactaaa tggattaggt catgaagatg
     4081 tactggaaaa atatgtgcca aagaaaaata tcttatttgg gatcacgatg tggacagctg
     4141 gtctagctgg tcccggaaaa gtcacattgt taggtgatgg agaaatcgaa ctagagaact
     4201 tggaaccaga aggcgaagca tttacaaaaa aagttgtaga agtctttcaa gaagcaaatt
     4261 taaatccgat ctatagccat aacgttcgtt attccatttg gcgaaaagct tgtgtaaatg
     4321 gaacattgaa tggtttatgc acgattttgg attgtaatat tgctgaacta ggcgcgcaaa
     4381 aagcggcaga gagcatggtt cgaacaatcg tatctgaatt tgcttctatc gcagcaaaag
     4441 aaggaatcat actagatcaa gaagaagtgt accaacatat tgcttctact tatgatcctg
     4501 acaacatcgg gttgcattac ccatctatgt accaagacct gatcaaaaac catcgtttga
     4561 ctgaaatcga ctacatcaat ggtgccattt ggcgaaaagg acaaaaatat gatattgcta
     4621 caccatattg tgcctttttg acccaattag tccatgcaaa agaaggaatc cttggcgcag
     4681 aataaaaggc tctctgatga gaatcagctg aatcagcagc tggttctctt tttttaagcc
     4741 caataaaaga tcaaaaagaa aacacaactc tcatgatgcc ataagaattg tgcttgtttg
     4801 tttcagtaca taatctcttc aaaatgtagg ttttttattt gttttcaaca gaggagaggt
     4861 catattcgta ttgctcataa atagtggaat cgttagtgat aaatcgtttt gcccaatttt
     4921 ctgcatcata aaattccaat agctctgata ggattggctt aaatggtgta tgcagaaatt
     4981 ccggtaattc atctaaatca cgttctcgaa gcaaaaaaat attttcagga cgataaaaat
     5041 gataatgagc aatcgcttga ttatccgtca ctagtttctt ttgaaaatac atggattcaa
     5101 atgtccgcaa agtcactcct tgctgaccct cccgcaacag ttccagaatc ccgcgcgatt
     5161 cccctgtttt tttcaggtac tcttcataag taagatatcc aggttgttca ttccctcgat
     5221 gtggaagaat gaaatagttg gtcgtaacat ctctttttat aaattcctgc ttatagcttt
     5281 ctgctcgttc ttttctccca ttattcgttg caccaaaaaa tagatccgtc tttggttcgc
     5341 tagaagaaaa attcatatgt ttcgaataaa atgaactatt atgtttcagc ccaagttctt
     5401 ttgcttccaa aaaatcaaaa tgataaatct catcgattgc cggttcatct tgtgcctgct
     5461 tcagcatcgc ctcgtgttct gctttgaagt gattccaaaa aaataacaca atttttgcat
     5521 tggtaaatgg gcgcacgcgt cgagccatct ccatatcaaa tccataatct aaaaaaacga
     5581 tcagctgata atttgccaac tgttgtctag ctgatttaat gaattccgac tgtagtaaat
     5641 cttttccgga ccaaacatct aaagagcgga tggaaggcaa aaaataaagt gatttatttt
     5701 ttgcaactag cagtacgtca tttttgttca tcttttccgc ctcactttct ctgtcacttt
     5761 ttcctatgat agcctgttta aatagtaaaa agccagtaaa acgtttccta tatttgtatg
     5821 aaaatttaac atttgtatga aaatatgatg aaataaatca ttcttctatt aaaattcgcc
     5881 taaaagtttt ctgaatagtt gtaattgtct gaaaatttgt tatcattagt cattgttaaa
     5941 tattgctatt tattcttaat ataaattttg attgtgaatt taaaagaaag aggtcattta
     6001 tggaagataa aaaattgaaa aaacaactta gctccagaca tatcaccatg ttagctttag
     6061 gtggagcaat cggtgcaggt ctatttaaag gaagtggcga agcgattggg atcgccggcc
     6121 cttctgtatt gattgctttt ttgattggtg ggctgatttt atatatcgtg atgaaaggtc
     6181 tgggaaaaat cgttttaagc ggtggcgaca cgcatcacgg attatctggt ttgatacgcc
     6241 cttatctagg tgctcattct gctgatttta ctgattgggt ctactggtct atgtggatta
     6301 tcaatatcat cgctgaagct gttgcagctg cttccttttt gcagttgtgg ttcccgcaca
     6361 ttccagcctg ggtatttgtt tttatgttgg ctgttttaac tacgatcatc aatctttatt
     6421 cggtacggtt attcgcagaa acagaatatt ggctggcttt tgctaaaatc agtgtcatta
     6481 tcttactgat catttttgct acttatcttg tcggtcagca aatgttgggc acaggcgttt
     6541 tccctaccct tcagcaattg actgaccatg gtgggtttac tcctcatggt atgaagggaa
     6601 tcatcagttc tttgcttgta gttatctatt cttacggcgg ttctgaactg attgctatca
     6661 cggtaagcga agcagatgat cctaaacgtg cgattcctaa agcaatcaaa ggtgtcatgg
     6721 gaagaatcat ttccttttac atcattcctt tgtttttgct tttgattctt tttccttgga
     6781 atactttagc aggcacgaat gtcagtcctt ttgtgatggt atttgaaaaa atgaatatcc
     6841 catttgcagc ggatatcgtc aattttgtga ttgtccttgc tttgttctca tctatcaatt
     6901 cgggtgtgta tgcatcttca agaattttgt tcttccgttt gaaggatcga aaaggttcct
     6961 ctagaaaact agctgtttta aacaagcatc aagtaccgca acgtgctgtt ttattttgta
     7021 ccagtgttct ttatcttggt gtagctcttt cttattttgt cggtgacaaa ttatttggct
     7081 atctggcagg ttccctttcg tatacagtat tattgatctg gatcattatc agtgcggcat
     7141 cctttgtact ggcactaaga aaaggaacat tatgggataa aggaatcagc ttttttgcat
     7201 tagctgtctt aggattgatt ttccttggta tcctgttcac gaactccatt ggggtaactg
     7261 tattgactgg cctgctttac ctgttcattt atttcagtta tcaaaaaaag aatgatgcgt
     7321 tcgtattgac gaatgaatga aatcgataga aaattttatt ttatttatgt ggaatagatg
     7381 gagctattga agaaaggagt tgtgacaaaa gtcttgttgc aactcctttt tccgaataaa
     7441 cagtctcatg acctcttggt aaacggtgtt caaaaaccct ctgtgcaaaa taattgattg
     7501 ttctccatgc tcatgacatg atagaagaaa ggaatgaaaa aaaggagttt gttttaaatg
     7561 aaaaagtatt ggaagttttt gttgatgatc ggtgtttcaa cggtagttat gtttggtatg
     7621 atgtacttga atgtctatgc gctcgatcat atcttcttca gtcgtacacg agtattcatg
     7681 gcgttgatga tgggtgcagt catggcagtc atcatgctgt tgttcatgtg gaaaatgtat
     7741 gaaaacaaaa agataaatgt tatgatctta gccgctagtc ttcttgtttt tgccggttcc
     7801 ttgtttatgg tacgaagcca aactactgtc aacgacacag cttggatgaa ggccatgatt
     7861 cctcatcatt ctatcgctat cctggcaagt aaacgagctg atttaaaaga tcctgaagta
     7921 aaaaaattag cggaagaaat cattcaggca caagaagaag agatcgacaa aatgaaaaaa
     7981 atgatcaaag aaatggaatg accattatcc gtccaaataa aaaaagagga tgttccagaa
     8041 gttatcagct tcaagaaata agaagaagct acttctggaa cgccgtgaat aaggggttgt
     8101 gaaaaagctg ccctgcatca agtaataaga acagccaaac aaaaatagct tctcctattt
     8161 ttcgtttggt taggcttatt accgcagatg caagcttttg aacaccgttt attaagaggt
     8221 tgtaaaatca gcgttctgac ctgagtatta gagagacaaa tggaaaaata gttcctcata
     8281 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tatcgagagg
     8341 ttgtgaaaaa gtcgcttagt ttcgcgtatt aagaaaaatt ttgtgaaatg gctttccaca
     8401 tttttgtata aatttggctt aataccgaga aactggcttt tgaataccgt ttattcgtaa
     8461 aagggattgt gacaaaagtc ttgtcacaat ccctttttat gtttttattg ttctaattgc
     8521 gctgtcttgc ttactgcttc ttgcggtttc acaaattttt ttgagattcc ccaacctgtg
     8581 aaggcactga ccatcgaaaa cacaggagaa agcaaactga aaaatacgaa tggcaagtaa
     8641 gtgagtgtag gaataccaaa tgtacttgct acgaaacttc cggcaattcc ccaaggcact
     8701 aagtagttga ttactgttcc accatcttct agcgttctac ctaatacagt tggatcaaat
     8761 cctgcttttt cataagtttc tttaaatgca ttaccaggca gaataacaga taggaattgt
     8821 tcaccaataa aaatatttac tccgatactt gtcatcaatg ttgtaaaaat caaatttggt
     8881 gtgttcgtca tctttcttga tactcgttcc atcacggttg agatcaagcc aaattcgatc
     8941 aacaatccac ctaatgacaa agttagaata atcaatgaca cagtaggcat catactttcg
     9001 attcctccgc ggctaagcag tgaatcgatt tgttggtttc cagtatttga aacaaaccct
     9061 gattcgatga ccccagccaa cttcgttaag gaagtttgtg ggttttgaat aaaaatcatt
     9121 cctactgcta cagcgatatt caacaaaatc gttaaaattg ccggcacctt tttccatgca
     9181 cacagtaaca ttaaaaataa aggaatcagt gaccagattg aaatagtaaa gtttgcttct
     9241 agaacatcag tcacttcacg aatagatgaa agacttgcgg ctttacttgt gtgtcctaaa
     9301 atcatgaata aaaagaaaga aacaacaaat gccgggacag ttgaccacat catatgcttg
     9361 atgtgtttga acaaatcggc atcaacaata gctgctgcta gatttgttga ttcggataat
     9421 ggagacatct tatctccaaa aacggcacct gaaacaatag ctccaacaac cagcgctgga
     9481 tttaagccta gtgttgtccc aataccgaaa aatgcaatcc caatcgttga cataacagta
     9541 aaggcactcc ctacagcgct acctacgatt gcacacacaa caaatacaga agggacaaac
     9601 cactggacac tgatcaattt gaacccaatg accattagag ttggaatgat ccctgcttgt
     9661 atccatacag caatcaatga accgactaaa ataaaaataa agattgggat gattcctggt
     9721 ttgatgccat tgatgattcc ttcgtgcagc caatctgtcg ggtatctttt tatcactgca
     9781 tacaacatga ccattccgat cgctgctaag atcgtgatat taggtgagac ctttaatccg
     9841 acaacgccaa tcgcaatgcc taaaacaata accattaata caagtgttgc ttctaaaaaa
     9901 cttggtgttc ctttttttcc catgataatc tctccttttt tagttgttct tatgttctaa
     9961 aaaagttaag atatcggatt actccgccgc aattcatccg cgattcctcc tttataaaaa
    10021 taaaaagacc tgttttaaac taaggacgaa tgactccgcg gtaccacctt aattaaaaac
    10081 agatctgttt ttcactttca aacttttatt caaatgtatg gactttccat gtaattcgtc
    10141 aattaggctt gatcggttca cagcgaccac cgactttctg aacaaaacac ctaaccgcta
    10201 ctgcgtgaaa agttgacttg tacgaagttt accttccata agatcatttg tcaatgaaaa
    10261 agaaaatatt ttttcatttt tctgaaaatt atttttataa ctttcacaca caagattatt
    10321 ttttactaga aagtagcgcc ctcttgtttt tttcttgcta ctatttcttg atgttcttgc
    10381 ttgattcttt ctaatagctt tggctctttc aacagatcaa gagctgtatt ggcgagtagt
    10441 tctgctgcaa tcgcaataga agcaaggcct ttgtcacttt tagcagcagc tttgaattct
    10501 tctgtgtgtc cggcaatata tgtctcagag attgctaccg taggctggat tgttggtatg
    10561 acttggctga catttcctac atcagaagaa ccgagacttg ttctttgttc cgtttctatt
    10621 tcttcatctg gaacacctgc ttcttttgca tgctggaaga ataagtcatc aaaagaagga
    10681 gtaacgatga catcatcaac tgcattttgg aacaatccaa actcaaaagt cgtgcccgta
    10741 cttatcgcag cacctcgtac gatattttcc actttttcat atactttatc taatgtttgc
    10801 cgatttcctg cacgtaaata gaaacgtcca gcagcgtaat ctggaatgac gttggcagct
    10861 actcctccgt ctgtaatgat cccatggata ttgacatctt ttggcaattg caagcgtaga
    10921 gcgttgattc cattaaacac ttggatcaat gcttccaaag catttactcc tttttcaggt
    10981 gctgctgctg catgggaagc gacaccgtaa aatttgatgt cgactggatc attcgctaaa
    11041 gattctgtcg ttttcccata acgaaaagct ggatggacgc ataacgctac atcgacatcc
    11101 tcaaaaaaac cttctcgaac aaagctgcct ttagctgatc cgttctcgcc cccttcttcc
    11161 cctggggtgc catatacacg gatttctcca ccagtttcat caatgaattg cttcactatg
    11221 ctggcggcta atacggagta agtaccaaac aaattatgcc cgcatgcatg accaatacct
    11281 ggtaaagcat cgaattctgc caaaaaggca attgtgggac cgggtttatt ggaaatataa
    11341 cgtgcatcaa aggctgttcg gtgtccagct acttcttttt ctacccaaaa cctttctttt
    11401 tctaattgtt taatcaacgc tgctgaggaa taaaactcat agttacttac ttcaggatgt
    11461 tcgtggatat ctagagcaag tgcttgatat tctggtaaac gatattggat tagatccttc
    11521 aattggtttg ttgagattgt tgttgtcata aaaaattcct cctatgctag tcatttttct
    11581 tattttctcc cgaatgtttc ccatgcaggc acgcttgatc cttttgatgt tttctcgatt
    11641 actttgactg tgtcttctgt ttgataagcg tctactattt tttgatacgt tttattgttt
    11701 tcatcttctt ttcttgcaac gatgatattc acataaggac gtgcatggtc atccactggt
    11761 tctaagaaga tggcatcaga agcaggatta aatccagcat ctacagctac accgctattg
    11821 atcactgaaa tgtctacatc tgacaaagca cgtgctgttt gggaagcatc taattcagaa
    11881 atgtctagat tttttctatt ctcggtaata tcagaaacag tgggcgcttg tccttttgcc
    11941 ttatccactt tgatcaatcc agcagtttgc aataacagaa gagctctgcc gccatttgta
    12001 acatcattag ggattgctac ttttgctccg tctttgatat cagctacatc cttcaaagtg
    12061 ttagaataaa ttcccagcgg tgcattgaca gtatttccga tcgatactaa atcagttccg
    12121 tgttctttat tgtaattgtc taaaaagaat tgatgttgga aagaattcaa atcaatcgat
    12181 ccatctgcta aagccacatt tggttgattg taatctgtaa atttcacgta ttccaaatta
    12241 atgttctcct ttttcaaacg gtctttcacg ttgtcccata catctgtatc ttctccaata
    12301 atccctagtt ttacgatagt ttcttttttg tcagttgtat ctgcttttcc ttggctacac
    12361 cctgccaacc aaataattgc tacaaatgta atgactacaa aacgaataat ttttttcatt
    12421 ttttctcctc cactttcaat gtgtaatttt tttaatcagt tgattactga taaattgact
    12481 taaaaaaact aagattagaa tcaataatgt agccacaatc gttacatctg tttggaatcg
    12541 attataccct ctcgatatag caagattccc taatccgcct gcacctactg ctccagccat
    12601 ggtagtcagt ccgatcacgt tgatgatagt caacgccgag acccgaatga taccaggcaa
    12661 accttctaac aaatacacac gtaaaatgat accgattgga ctggttccca tcgcttctgc
    12721 cgcttcgatc accccaggat caacttctag cagtgcgttc tcgatttgac gagcaaaaaa
    12781 cggaacaact ccaaaaatca atggcactaa agctgctttc gcaccaatcg tcgttccaac
    12841 taagaaacga gtagtcaatg ctaaaagaga aagcaagata atgaaaggga tggaacgaat
    12901 gatattgatc agcttatcaa gtatttcaaa tagcagacgg ttagctagta atccattagg
    12961 gcgtgtcact actaacgtca cacctaagat gattccaatc atcccgccaa tgaatgctgt
    13021 ccaaaaagac atatatagtg tttgcttcgt cgcttcgaca aattcatccg gaatcaaata
    13081 aacattgggc aaatatgttt taaagaaatc agtcatttta tcttcctcct tcgatcactt
    13141 tgatacgact atctaaatcg tttttttgta aaatatttat ttttacacca gatttgatca
    13201 aataatccaa tgcttttttc cgctgtttgg tttctccagc taaagtaacg atcaaactcc
    13261 ctaacggcgt ttcttgtaat aactctacat tgccatataa aatattcgct gttacttgat
    13321 atttgctgta cagatgagcg attagaggtt cgttcgtctg atttccaata taacttaatt
    13381 ccaccagcca tttattggcg atctgatcgg caaaagcaga atgggcaatg atcgtttcta
    13441 atgcttggtc aagatgagta gctgttcgaa taaaatcttt ggtcaattct tcttcaggat
    13501 tgctgaagat ctggatgctg ctaccttttt caacgattct tccatcttcc ataaccgcta
    13561 ccttgttgca gatttctttg acaacttgca tctcatgagt gatcaggacg attgttaaac
    13621 ctaactggcg attcaatttt tttaacaatg caaggatctg aagggtcgtt ttcggatcaa
    13681 gtgcactggt tgcttcatca cataataata ccttaggttc attcgccaaa gctcgtgcga
    13741 ttgctactcg ttgtttttgt ccccccgaca gttgtttagg ataagcagat gctttttctt
    13801 ccaaaccgac aagttccagt agttcattca ctttttgacg acgttcctgt ttcgattttc
    13861 cagaatactt taaagaaaaa tcaacgttgt caaaaatagt tcggctttcc attagattga
    13921 aatgctgaaa gatcataccg atggcctttc tttcttttcg caattctttg ggagagagct
    13981 gactcagatt cgtctgattg acaatgactt ctcctgctgt tggtttctgc agcaaattca
    14041 tcacacgaac taacgtactt ttccctgcac ctgagtaacc gacgattcca taaacatcgc
    14101 ctttttcaat cgttaaatct acagaatcta ctgcctgtat ctctttttct ttttgtttga
    14161 aacatacaga aacatctcgc aattcaatca ttaaaattcc tccttatcta aaaaaagcgt
    14221 cctcaagaaa atgaaaatgt cattttcttg aggacgcttt tttgcgtgtt accacctcat
    14281 gttcaagctg tgttcacaca caactcctct tcaagtacga tcatactcta gcaagatatc
    14341 ggtcgcagcc gtcatcgcct tacatctatg ctcgggatga attgctcgat ggccattttc
    14401 caatagtcgt tccttgcttc ttctcagcta ccgaagctct ctgtcaaagg ttcttctatc
    14461 gtacttacca tttcaatgca aataatattc aaaaaaaaag tccttttact aacataaact
    14521 atgtcagtaa aaggacgacg tatcgcggta ccaccttttt tcggaagttt ccttccctca
    14581 agcccaacat ctcaaaaaga gagattggcg ttcgttttaa cggtcgaaca gttccgaatt
    14641 tgcctactta tcgacaaatt attctccaag gccattttca gcttttctta cttgactctt
    14701 ctcaccagcc agagcctctc tgcaaagttt ccaaacttac tttccttttc aacgaaatcg
    14761 atattttgtt gttgggatta atctacatga ggaaaactga aaagtcaagt acgaaattca
    14821 aattattttt tctatattta tttttctgtt tttttattta acatttcaat aacagttcga
    14881 agatcttttc cttccgcaag aagtttcaat ttttcctttt cttcgacggt ataatttcgg
    14941 acaaaactgg aatcaggtat caaaacaacg cctaatcctg ctcgtgaagc agctgttgct
    15001 cctactaaag aatcttccac tgcaaaggct gaactagctt ctaatcctgt tcgttccaaa
    15061 gctgtcagat aaggaatcgg tgaaggcttg ttctccgaca cttcgtctcc gaatgtaaac
    15121 gtgtccaagt aatcagctaa tttaaagtga tccaagatat ctgttgctct tttcgtaaca
    15181 gtggtcgtag caatgcctag tttcatatga cgttctttta agtctgccaa tatttctttt
    15241 gcatatggtt ttaagtggat ggctccgctt gtcagctgtt gataaaaata ttcttcacgc
    15301 ttcgctcgga cttcctgtac tttttcaggg ctacctgctg ctttcactaa aatatcaaaa
    15361 ctttgtttcc agttttttcc gccccaagcg tcaacgattt cttttgggat atggatatca
    15421 aattgctgaa atccccaaag ccagccatca cgatacgttt tttctgtgtc aactaacaaa
    15481 ccatctaaat caaatattgc acctttcatc tatacattaa tcctttccaa aacaatacac
    15541 atagtttagc acgactggtc caaaaagaac agaagcaaag aaccagcctt ctcgtttata
    15601 agagttcttg gaatcgacgg atatagattt tcttcacagc tgtcacaagc gccagataag
    15661 cgatgactgt agcaaataac cataaccagt aattgccagg aagtggcatc aagcctaatt
    15721 cagcaccaaa acttgtaaaa ggaaggacag aaccgacaat aattcctaat gtagtgataa
    15781 caaacatggc aaatgttgcg ttgctttgga taaatggtac tttcggtgta cgcagtgcat
    15841 ggagcactaa ggtttgtgtc catagtgatt cgacaaacca tcctgcatgg aataatgcga
    15901 tgaaaatcac tcgctgtgaa ctatccaatg tatggaaact tccgcccact acagcaggac
    15961 aaataacaaa atacatcaac aagtaagtag taatatcaaa tatcgaactt gttggaccaa
    16021 agtaaaccat gaattcccca atactagatg cattccattt ttttggctcg tccaaatatt
    16081 ctttatccat ccgatcccat ggcaaggaaa tgcaggaaac atcatagatc agattcaaaa
    16141 acaaaagttg taaaggcagc attggtaaaa atggcaggaa cgtacttgca atcagcacgg
    16201 aaaacatatt gccaaaatta gaacttgctg tagccttgat atatttcatg atattcccaa
    16261 aagtttctcg tccagaaaga aggcctcttt ccaaaatcgt caaatctttt tctaaaagga
    16321 tcacgtctgc agattctttc gcaatatcca cagctgtatc tacagaaata ccgatatcag
    16381 ccactttcat tgcaggagcg tcattgatac catctcctaa aaatccgaca gtatgaccat
    16441 ttttacgtaa agctgtcgtc aaacgtgctt tttgctgagg attcaatttt acgaatactt
    16501 catggtcttc agctacttgt gctaattcat gatcactcat ttccgagatt ttctcaccgg
    16561 taaccaattc attgatctct agacctactt gacggcacac tgaacgtgta actaattcgt
    16621 tgtccccagt cagaactttt acagctacac catgttcttt cagagctttc aacgcttgtt
    16681 tcgttgtttc ttttggtgga tcaaggaaag caagataacc aatcaaaacc atgtctgact
    16741 catctttgac tgaaaattct ccaacgacgc ttggattggt cttttgtgcg acaccaatca
    16801 ctcgtaagcc atcttcatta agtgactgta cttttgcaag tacggcttct tttcgttctt
    16861 ttgttaatgg tgtaacagta ccgccaagat caataaattt agaaatgttc agcatttctt
    16921 caatcgcacc ttttgtgatc atttgtgttt taccagctgg atcagcaacc acaacactca
    16981 tacgacgacg ttcaaaatca aaaggaattt catccacttt ttgataattg atcttatctg
    17041 tatctaaagt ttctgttgct tcatcaatga tagccacatc taacaaattt ttcagccctg
    17101 tttgatagta gctatttaga tacgcatggc gcaaaacacg atcatcttca tttccttcac
    17161 aatccatatg atattcaaga atgattttat cttgtgtcaa tgtccccgtt ttatccgtac
    17221 ataacacatc gattgcacca aagttctgga tggcattcaa atttttgatc actgttcctt
    17281 ttttggccat cacacttgct cctttgacca aattcgtcgt aacgatcatt ggaagcattt
    17341 caggtgttag accaactgct acagatagtc caaaaagaaa agcttctagc caatcccctt
    17401 ttgtaagccc attgatgacg ataactgctg gagccatcaa tgacataaat ttgatcagta
    17461 gcatggatgt tttatggatg cctaattcaa agcttgattg aattggtttt tcagatagcg
    17521 tttctgctac atgaccaaat agtgtttcat ttccaacaga aacaatgatt ccttccgcac
    17581 ttccactgat gacattactt cccataaaga caagattttc gtaacttgtt tcgctttgct
    17641 gatcgtcttc acatactttc gcatgctttt ctactggata gctttcccct gtcaatgctg
    17701 cttgggaaat gaataaatcc tttgcggaca ataaacgcat atccgctggt atcatatccc
    17761 ctgctgaaag ccgaacaaga tctccacaga cgatttcttc cataggaatc tctgtgtact
    17821 ttccattacg ttttacagct gccgttactt tcacaagtga tttcagtttt tcagccgctt
    17881 gattggaacg tacggactga atcaacgtca ttgtcccgct gacgataacc atagtgaaga
    17941 taatggctac accgaaaaga tctttatctc caggagctgc taatacatat tccgtgatga
    18001 atgaaataat tcctaatgcg atcaacacta atgtaaatgg cgtgatgtat gctttcaata
    18061 tttctacaat gaatggtgtt tttttgccat atgaaatcgc attttcaccg taattttctc
    18121 ttgaacgttc gatttgttct tctgtcaatc catttgctga tgcatggaat agttcaaata
    18181 attcttgttt cgtttttcca gcaaaagctg catagatttt ttgtgtatct ttcttttttt
    18241 ccatctcaat ctctcctttg tgtccaaaag acagctgttc tcgctgcttt tggtctttag
    18301 ctattcatcg gatcgataga gaaaaaactg aaaggggctg agaacagata gataatagcc
    18361 ctggcctttc ctctagatcc ttgtttttcg aatgactgtc atccatttgt tctcatctcc
    18421 tttactaagt aattttaatt catgacttcc aatcattttt cgaatagtta tttttccaat
    18481 tagaccacct cctttttatt tttcaccgat ggggatttgc ggcaacaaaa aaacctctcg
    18541 ccaaatactc agcgaaaggt ttgaaaaagc atattaaaaa aagcttctca gatctcatcg
    18601 ttgagttttg acactgtaca acgtaaaaag cagatacctg cttttagcta ccttatgaaa
    18661 agccttatct cgacaattcc tgttctaccc attggcgtct ctcgacgttt ttgggcagta
    18721 gcctatgttt gtataggagc ctcacctaac aaatcttatt cggttttcct tcgttaatat
    18781 aacaactaaa aaagaggatg tcaaatcaat tttccatgtt tttcatactt tttttaaaat
    18841 acacacgtta tattcctgtc tcacgcggtt ttatttgtct cattttccac ctcccgctat
    18901 aatagaaata tagtaggaaa cgaggtgctg ttatggaaag aacacttgtt attatcaagc
    18961 cagacggcgt gagacgccat ttagtcggaa gcatcatcca acgttttgaa gcaaagggac
    19021 tggccattgc tgaaatgaag tttgaaacta tgactcctga attagcaaaa gaacattatc
    19081 agcacttgac tgagcgcagt ttttttgatg aactgatcga ttacatgact tccggtcctg
    19141 tcgtttatct tgtgctggtc ggcgaggaag tgatcgatat cgtccgtaag atggttggag
    19201 caacaaaagc tgccgatgct gttccaggta cgatccgagg agactacgcg ctgccgggga
    19261 ctgaaaacat cattcacgct tctgattccc gtgatgctgc ggtaaaagag atcgcccgct
    19321 ttttcccgcc attcgatata aacaaaccgt ctgaaaaagc ggcgaagtga tacaaattct
    19381 aaactgctgg tctagttgct gggttcagca atggccagca gttttttctc taaggacctc
    19441 cgttgattct ttcctaacat atattcgagc actgtgaaga attcttggta gtctttcgtc
    19501 ttttcttccc ctaaagcagc agaaatcaat ctttgataat aatctgccat caatgtttca
    19561 tagatcactg tacttctttc tttatgtgct aagctccgtt tcatatcttc attcacacta
    19621 cgtgcctttt tcaaccgacc attttctata taaaacgaag tcaggttcat taaaaaagta
    19681 aagagatact gctcatgatt gttcaactgt gctaatcgtc gcattctttt tttgattttc
    19741 aaataattgt tctcaatgaa ccgatcactg aatatccaag gaaaatcagc tagaatagat
    19801 agttcaaatt gaccatagtc tggtatctgc atcaagtatt cttcaatgat tcggcattca
    19861 taacgtaaaa attcaaattc tgtctcagat acttctgttt tttcgaccat gtgaagattc
    19921 aaaaccaaat aatacagatc ttctgttttc ttaaaatgcc tacggttatt gctgatctct
    19981 ttataaggat caaagctgtc ttcttgtacc cgatctagca ttcgtctttt tttctttaac
    20041 tttattggtt catccagctg gtttcgcatc cattcatatt ctgcaagtgt catgttcatt
    20101 cgctctaaaa atgatctcaa tgttacaaat gacatttttt tcactgaatt tgtctcaatc
    20161 ccgactagag aagtcttaaa acagattcct tccgctaatt gggactgtgt caacttcttg
    20221 ttgactcgaa gtttcttgat caattcacca tcatacataa tagttctcct ttttaattta
    20281 aatttttcca cattcaataa atagatacgt taaaaaggaa aaatacacga aaaaaataaa
    20341 aaaatgtttt tttcataaaa aataccaacc ttcaagcatt agaatgttgt ctaactactg
    20401 aaggttggtt tgattgttta ttgattattt ttttgctgtt caacgtctgt tcttgttact
    20461 ggatataagt tggttgttgt tttattacca ttttttgaaa agacacttgt cgaatgttcg
    20521 cctttttgct tttcgattgc ttcattacgt tccgcttcat ttttatagtc atattttttc
    20581 ggatcaatcg tctcaaatcc gtttggcaca taaaaccgca agagattttc ctgattcagc
    20641 ttatctgaaa gacttaacgc agtcttgact ttttcctgat ctttggagat ttcctcatcg
    20701 attcctgctg ctttcgtatc gatgatttct cctgtctgat tattgtaagc ttttcctcct
    20761 aggacggtgt atttgggtgt cacaaagttc ccatttcgaa aagcaactac ttgatcatgc
    20821 tgagaagata ataagtctgt accaaaatga agataatctt tatcatcgat tcccaataga
    20881 tgtagtaatg tcggcaaaac atcgatttct ccaccgtatt catgattgac tgtcccttta
    20941 gtataaccag gcatatggat catcaatggc actcgttgca tctgtgcgtt gtcaaattca
    21001 tcccatgtat ctggatcttt accaagagcg cttgctaaat ctttattatc tgtattcgag
    21061 atcccaaaat gatccccgta aatcacaaag attgaatttt ggtaaatacc agattttttc
    21121 aaatacgtaa aaaattgttc caatgattga tccagataat gtgccgttcg aacataatta
    21181 tctactgtgc tattgccagt attcaaagat ggaaagtcga aattcttgtc atccgtataa
    21241 taaggcgtgt ggttggttac ggaaagaaat ttcgtgtaaa acggctgttg tagatgctca
    21301 agatattggg cagattctct gaataaatct ttatctaata tcccataacc taacgtttcg
    21361 tcggattcat caaaataaga tcgatcaaag aaattgtcat aacctaagtt tttatataca
    21421 tgatctctat tccaaaaact agctacattc ccatggaaaa cagcgcttgt atagccttgc
    21481 ttttgtccaa ggatcgctgg tgcggcttga aagacattgt ctgacccaag ttctgtgaat
    21541 aatgagcctt gaggtaaacc aaatgtgcca gtttccaaca tattttcagc atcacttgtc
    21601 tttccctgtc ctacttcatg aaagaaattg tcaaagctga tcgttgcttg attctggaaa
    21661 atactattta aaaacggtgt gacttcttgt ccgtctacct tcatattaat caaaaactgc
    21721 tggaaacttt ctaaatggag aacgatcaca tttttccctt tagcgatccc aaatgtttcg
    21781 ggatttggct ctgcataatg tttctttgta taatctaaaa cttcatcaat cccattactg
    21841 cttgcatgcg cgcgaacact gctagtcatt cctgttttga ttccgtcata aatagtatac
    21901 gcatcgattc ctagatattt gacaatatat gaacggtcaa atgttcgctg cagcaattgt
    21961 ggacggttgg cttcgctcag cgctagattt cctgaaaaaa taagccctga aagacaggta
    22021 acagcgatag ccatcggttt gccgacaggg ttgcttttga taggtatttt cttgaccttc
    22081 aaccaaacca gtagcccaat gaacacagcg atatccagcc agtagatgac atcatggggc
    22141 ttcatcaaag aaaaggagct tcctgacaat ccttgcgaca ctttcgaaaa gcctaataca
    22201 gatttgactg taatgaaatc tgtgaattcc cgataaaaaa taatattcaa gtacaacaaa
    22261 agcgtattta aaatatcaat gatcaaaaga atagctaacg ctggctttgg ctttttaata
    22321 tacaaagaaa ggccaaaaaa caataaggta gtcgcaattg ggttgatcca caagataaag
    22381 tactggatcg taccttccac acctaatgaa aaatctgcat agtaggcaat gatcgtcttc
    22441 agccagaaaa agaaaacagc tgtcaaaacg attgctaaac gattttggca caatttttta
    22501 agttgtgtca cgtatatcac ttctctctat tcacattttt tcttattttg ctcctgaaaa
    22561 atgaaaattt cctaaaaaag ttgttatctt aagatttttt tattctttct aaagattatt
    22621 ccgtccactt ttcacagcat aaaaccgctg cgatgtaagc gattaaacta atgaagagat
    22681 aaaagtcttg tggtgaccgc ttcactccac ggtattcaaa agccagtttc tcggtattcg
    22741 tacaaaaaaa gaggctgtga caaaattctt gtcgcagctc cttatttctt gatgcaggac
    22801 agctttttca caacttcttc cttaaacggt gttctcctgc caactcctcg gccacaagtc
    22861 acaatattta taaaacatga gaagctgttt tttgaaattg ttccttgtgg ctcagagtta
    22921 aacggcaggc tcaagacctc ttgatacacg gtgttcaatc agctgaccct cggtattaga
    22981 ttatgtcagg acgctaattt tacaacctct tggtaaacgg tgttcaaaag cttgcatctg
    23041 cggtaataag cccaaccaaa cgaaaaatat aaggagctat tttttgaaat tgctccttgt
    23101 ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc
    23161 ttcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttgccgtt tgtttctcta
    23221 atactcaggt caaaacgctg atttcacaac ctctgttttt cattgtccat aggattgata
    23281 ttgtccttgt gatgactcat tcgtaataat tgaggcttga ttttcttcac ttcccgttat
    23341 cgtttgtcca ctgccagaat ctgaaggact tgttcctgtc gactcgtttt tttgagaaga
    23401 gacagttgtt ttcttgactt gttcatttgt ctttttccat tccgctaaac gatcggcgac
    23461 cacttttttg tctacttttt cagaataagc ggtgatcact tttccatttt tcaaacgggc
    23521 tactttgtcg ccatcatgag tcaaaagcaa aacggtcaaa tgcttgtctt taaattgata
    23581 ttgtctgaga gaaatcgcac taagtacgtc atcgatatta ataaattgat tttcttcttt
    23641 tccttttggc aaataaatgt ccaattcgcc tttttcgtaa ttggcagtta ttttatactc
    23701 cgcccaccaa taagttgaga attctttttt gaaattatct actgattctt tgcttgctac
    23761 gattttttct gtttctttct gagcaagatc tgctgtcatt tttcctgaaa atgctgcgga
    23821 taaaataagc agtaagaaaa aaccgaataa ccaaaagaga agaaacggtc tttttttcct
    23881 catgctttcc tccactccta tttaattttg actattctat tatatacaaa aaatgaaata
    23941 gaaagacaaa aaagttgaaa aagatcgtaa ataattgaac ttctttttca caaaatggta
    24001 agaatggaga atttcacgca tttgctctca actattcttt cttaagcctc aaatccgttt
    24061 ttatctgata cgtctttgaa ccaatgaccc gattttttga ttgttctttt tccatcttgt
    24121 gctaagtcta ctgaaataaa gccatatcga ttcttatatg cattggtcca cgaccagtta
    24181 tccatacatg tccacatatg atagcctaaa caattggcac cttcttgaat ggcttgatgg
    24241 acatatttca gatgagattg gacaaattcg atccgataat catcgtggat catgccatct
    24301 tctttgataa atcgtgcttc gttttctaca cccataccgt tttcagaaat aaagcagtcg
    24361 atatttccat aatgatctcg tacattgatc aaaatatcat agattccttg ttcatagatc
    24421 tcccatccgc gataaggatt cattttacga ccaggcattt cataattgtc gaaataatcc
    24481 tcaggcattg gaccgttttt agtatcgatt ggtgtttctt ttgcttttac ccggcgtggc
    24541 tggtaataat tgatacctaa aagatctact gtattttctt tgataaccgt caaatcctct
    24601 gcatggatca caggcatgag atcaagttct tttactatct caatcaattc acttggaaac
    24661 tcgcctttta cagctggatc aaggaatgaa cgattgaaga acgcatctgc tatttgcgct
    24721 gctttaacat cttcagggtt tgattcgtct cttggatagc ttggtgtcag attcaagatg
    24781 ataccaataa caccaccttg ctccatttca tggtaagatt tgatggctaa cgctgaagcc
    24841 aacgcttcat gatagccaac ttggatcgct tttttcatat ctgactcttc tggataatgc
    24901 catcgatata aataacctgc ttcgactgga acgattggtt cattatgagt aaaccatttt
    24961 tttactttcg aaccaaacaa ttcaaaacaa cttttggcat atttttcata ggcttcaacc
    25021 gtttctcggt tcaaccatcc gcctttttct tgcagataca tcggcatatc gaaatgatac
    25081 agattcacaa agggttcgat accgttttca atcaactcat caaaataacg atgatagaaa
    25141 cgaacggctt cttcgttgat tgttccagat tcatcaggta tcaaacggct ccattggata
    25201 gatgttcgaa atgaattatg cccagtctgt ttcatcaact gtacatcctc ttgatacttc
    25261 gtataaactt gcgaggtttt ttcaggtcct acttgatcaa agaacttttg cggttctttt
    25321 ttgtaccaat aatcaaaaat gttctctcct tttccatcgc ctgaaatcct tccttctgtt
    25381 tgtgggccac ttgctgcaga accccaccaa aaatttgctg gaaattgata ttttttcatc
    25441 aaagcacctc tttttctttt ttagttcgtt ttagttgatt ccatgtatga tattttccat
    25501 cacaatcact ttttcatttt cttcctcttc ttttttgatt cgttttaaca gctgttctgc
    25561 tgctcgttcc ccgattttat ctgttggtgg cgtgatcgtg attttcggaa ggatactcag
    25621 atcgttttta tctaaataat catagtgact gaacgcaata tccttcccga ttctgacacc
    25681 ttcttgattg aaacattcga tggcaccaat cgacatattg taattgctga caaaaatagc
    25741 agttgcccct ttttctaata atgcttttgc cccgtaatag ccgctttttc tagaatagtc
    25801 tcctgtaaag acctctaatt ggatcgatgg atcattttgg accgcttctt tggctcctgc
    25861 taaacgttct cttgctacgt agtctgtttg agtggcagca ataaatccga tttttttatg
    25921 tccttgttca ccatgatgag cgatcacttt tgctacactt tctcgatcat tcactaatat
    25981 tgtatcaata cctgctggtc cttctggaca attcaatgcg ataactggaa tactcatttt
    26041 tgtgagcatt tcaaatccag accactcttc ttcggataag aagacaatca gcccatcgat
    26101 cacatgcttc atcaggtatt caattttttg ttcaattttt tctggctgat cggcaaaccc
    26161 gcttaataat aagctatacc cattttcttc agctactctt tcgatccctg agacaacttc
    26221 cgacccaaaa cggctggaaa tcccgtttat caaaagtcca atagagaagc ttcgattatt
    26281 cttcaaccct ttagcaatga tgttttcttt atagtccaac gctgaaatcg cttctgctat
    26341 ttttttcatg ttcttttcct tcagttgctg accattcaag taacgggaaa ctgttcccac
    26401 tgaaacacca gccatcttag ccacatcacg gatcgatgcc atacattctc actcctgcca
    26461 gttacatttg atttctcttt cttgttgttc gattatgatg accgcattct ttgcatagtg
    26521 ttgcttgtca tcaatatcca tataactaaa gtcttcagta gagcaacttc ctctcactat
    26581 taattatact taaacaagta tttgaaccgg ttcattttta atatagcatt tttttaaaag
    26641 attgcaacac ttttttaaaa cgctttctga aagtggcgga ttttttgcct ttttaaaatt
    26701 ttattctaaa atttactcta tttgtgggtg ttttgtgcgt ttttctcttc catccgtttg
    26761 aagttttaca ttccaatggt ataatttctt tatcctatca aatgggggaa gtattttgaa
    26821 gaaattaatc gtactattta ttttgtctgc gggacttttg acattgactg gctgtgattt
    26881 gtttggcggc tctgaccaat cctcggacga aacaaccgat tcttctgtct taaaagcgga
    26941 aaaagcagat accaccaaca gtgtatatac gctaaaagta cgtaaagata tggatttatc
    27001 cgcgggacag ttgacattta cagataaaaa aatggaatgg acacgcacgt atcagacaca
    27061 aggaactgat tcttctgaat catctgaaga aaaaacagtg ctgacagata tcaagatcac
    27121 taccaaagat gacgaatata ttatttcagg aaaagaaaat aaaaaagaaa caacagtcat
    27181 atttacaaaa atcggtaatt accgtttgaa ggatgaggat ggcaacatct acagtctata
    27241 gtgccgtctg ttggatcttc ttttgtttct ccccttcttt tctgctatag taaagaagca
    27301 agatgattgt aaaggagacg aaatggatga tcgatagtcc tgattattgt acatgtaata
    27361 acaacaaaaa tatccataca gaatttaatg accaaggaag cttgagcata tgcgacaact
    27421 gtcacaaact agtcaaagat tcatttaaaa catttgaatc ttatcctgtt caaaattatg
    27481 taaaaagtag aagaagataa acaaatccaa gcaaaactca ctatggctga tttttatgaa
    27541 cttcaaactc agcttcttcc tttgatcaag cttcatcagt aaaacaaaca gtggatcatt
    27601 tcagtttaca aatcaaagag taaagattta tttatttcaa atatttgaat ataaaataac
    27661 caaacgatag aaaggcttat tctacatcac gtagta
//
LOCUS       DAZKOW010000027        27672 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000027 DAZKOW010000000
VERSION     DAZKOW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.027"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(256..2148)
                     /locus_tag="ACG912_001144"
     CDS             complement(256..2148)
                     /locus_tag="ACG912_001144"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512477.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2145..3872)
                     /locus_tag="ACG912_001145"
     CDS             complement(2145..3872)
                     /locus_tag="ACG912_001145"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512478.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3888..4538)
                     /locus_tag="ACG912_001146"
     CDS             complement(3888..4538)
                     /locus_tag="ACG912_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFQ0512479.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4742..5038
                     /locus_tag="ACG912_001147"
     CDS             4742..5038
                     /locus_tag="ACG912_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0512480.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5210..5830
                     /locus_tag="ACG912_001148"
     CDS             5210..5830
                     /locus_tag="ACG912_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0512481.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5922..7088)
                     /locus_tag="ACG912_001149"
     CDS             complement(5922..7088)
                     /locus_tag="ACG912_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0512482.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7100..7453)
                     /locus_tag="ACG912_001150"
     CDS             complement(7100..7453)
                     /locus_tag="ACG912_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0512483.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7581..8603
                     /locus_tag="ACG912_001151"
     CDS             7581..8603
                     /locus_tag="ACG912_001151"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFQ0512484.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8622..8783
                     /locus_tag="ACG912_001152"
     CDS             8622..8783
                     /locus_tag="ACG912_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512485.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9042..9476)
                     /locus_tag="ACG912_001153"
     CDS             complement(9042..9476)
                     /locus_tag="ACG912_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512486.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9586..11445)
                     /locus_tag="ACG912_001154"
     CDS             complement(9586..11445)
                     /locus_tag="ACG912_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0512487.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11446..11586
                     /locus_tag="ACG912_001155"
     CDS             11446..11586
                     /locus_tag="ACG912_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0512488.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11563..12723)
                     /locus_tag="ACG912_001156"
     CDS             complement(11563..12723)
                     /locus_tag="ACG912_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0512489.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13030..14454)
                     /gene="araA"
                     /locus_tag="ACG912_001157"
     CDS             complement(13030..14454)
                     /gene="araA"
                     /locus_tag="ACG912_001157"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFQ0512490.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14470..15168)
                     /locus_tag="ACG912_001158"
     CDS             complement(14470..15168)
                     /locus_tag="ACG912_001158"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0512491.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15125..16786)
                     /locus_tag="ACG912_001159"
     CDS             complement(15125..16786)
                     /locus_tag="ACG912_001159"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFQ0512492.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17004..18404
                     /locus_tag="ACG912_001160"
     CDS             17004..18404
                     /locus_tag="ACG912_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFQ0512493.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18656..19735)
                     /locus_tag="ACG912_001161"
     CDS             complement(18656..19735)
                     /locus_tag="ACG912_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0512494.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19821..20780)
                     /locus_tag="ACG912_001162"
     CDS             complement(19821..20780)
                     /locus_tag="ACG912_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0512495.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20792..22303)
                     /locus_tag="ACG912_001163"
     CDS             complement(20792..22303)
                     /locus_tag="ACG912_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0512496.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22325..22888)
                     /locus_tag="ACG912_001164"
     CDS             complement(22325..22888)
                     /locus_tag="ACG912_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0512497.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22894..23757)
                     /locus_tag="ACG912_001165"
     CDS             complement(22894..23757)
                     /locus_tag="ACG912_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0512498.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23771..24667)
                     /locus_tag="ACG912_001166"
     CDS             complement(23771..24667)
                     /locus_tag="ACG912_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0512499.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24842..26131)
                     /locus_tag="ACG912_001167"
     CDS             complement(24842..26131)
                     /locus_tag="ACG912_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0512500.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26103..27620)
                     /locus_tag="ACG912_001168"
     CDS             complement(26103..27620)
                     /locus_tag="ACG912_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFQ0512501.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT         8070 a         5844 c         4790 g         8968 t
ORIGIN      
        1 gctcctccta tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
       61 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagata caaaacggaa
      121 aaatagcttc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
      181 ccgtttattc ggaaaaaggg actgtgacac gacttttgtc acagccccta aaaaaaataa
      241 taaatttgta ctattctatg aagcttcttc ttcagaaaac tgactgttat aaagatctgc
      301 atagaatccg tctttttcca tcaatgtatc gtggtttcct gtttctacga tcgaaccatg
      361 attcatcaca ataatgttgt ctgcatcgcg tattgtagac aagcggtgag caacaacgaa
      421 acttgtccgg ttttccaata ggcggttcat cgctttttga atcagtattt ctgttcgtgt
      481 atctacacta gatgttgctt catcaaggat caggacatct ggatttgcta agaatgcccg
      541 cgcaattgtg attagctgac gctgcccttg cgaaatattg cttgcatcct cgtttaatac
      601 agtgttatat ccttctggca atttacgaac aaaatcgtct acgtgagcag ctttagcagc
      661 ctcaatgatt tgttcgtctg ttgcatcgtc gttcccatat ttgatattgt catagatact
      721 tcctgtaaac agccaagtat cttgcagtac cattgaaaac tgtgcgcgca attcatcacg
      781 tgaaagatca cgggtatcta caccatcgta acgaatacta cctccagaaa catcgtagaa
      841 ccgttcaaga aggttgatca gtgtcgtctt ccctgcacca gtcggaccta caatagcgac
      901 catctcacca ggtttgacat ccaagttaaa atcagtcatt agcaagttat ctggtgtgta
      961 gccgaattga acattttcaa aacgaacttt gtatggtgta tctttttctt ctggaaggcc
     1021 agatttttca ttggacatct cttcttcatc caacacttca aatacccgtt cggctgaagc
     1081 aactgttgat tggatagtgt tcatcaaact tgcaagttga gtgattggtt gagagaactg
     1141 tgttgtatat tgcaagaacg cttgaacatc ccctaatgtc atgttcccat ttgcaacttt
     1201 tactccgcct agtacagcga cgaacacata gcctagattt ttaacgaaat tcatcaacgg
     1261 catgatgatt gccgaaataa attgagcttt ccaaccagcg gcatacagat tctgattttc
     1321 tttttcaaat acttcctggt ctttttcagt atgattgaat gttttaacaa tggtatgacc
     1381 accatatgtt tcttctactt ggttattcaa taaacctaaa cttttttgct gatctgcgaa
     1441 aaacacttgt gatttcggcg ctacgatcat cacgatgatc aagcttaaag ggatcatcaa
     1501 taacgcgact aacgtcaatt gccagctgat tgaaagcatc atcacgatga ccccaatcaa
     1561 tgtgactgca ctagttacaa attgagtcaa attttgctgc agcgtgcttg cgatgttatc
     1621 catatcattg attgctcgag acatgatatc cccatttgta tgtgtatcat aataggaaat
     1681 cggtacacga ttcattttgg cttccaaatc atgacgtaat tcataaactg ttcgttggga
     1741 aacacgtgtc ataataaatt gctgtaaaaa gttgaataca gcagaaatca aatacatcac
     1801 gattactgtc gcaatgatct gcgcgatttt atcaaagtct atcggaaatt ttgttacttg
     1861 ttggccttgt tgcatctgtt gagcgccagc cattacacct ttgaatattt ctgtggttgc
     1921 tttccccaag acctttggtg tttggatttg gaagatcacg gctgctattg ctagtacaaa
     1981 tacagcgatg atcgcaacca tacgtttaga catatagcca aacaatcggc gtaccgttcc
     2041 ccagaaattt ttaggctttt caccttttac accgagattt ctgccaggtc caccatgtcc
     2101 tggaccgcct gctggtccat gagagggacg tgtgtttgtt ttactcatgc taagtcctcc
     2161 tctcttagtt gtgaatgaac gatttcttga taagtttcgt ttgttgctaa tagttcttca
     2221 tgtgttcctt tgccaaccag ttttccttca tctagcacaa ggatcatatc tgcatccatt
     2281 actgtactta ctcgttgagc aactaaaacg gtgatacttt ctttcatatt cgttttcaat
     2341 gcttggcgaa gattggcatc cgttttgaaa tccaacgctg agaaggaatc atcaaacaca
     2401 tagatatctg ctgttttgac taatgcccgt gcaattgcca gacgctgtct ttgtccacca
     2461 gagaagtttc ctcctccttg ttccacatgg ctatctaagc catcttccat ctctgaaaca
     2521 aaatctttag cttgtgcgat ttctaatgct ttccaaatca tttcatctgt tgcatccggc
     2581 ttaccatact gcatattgtc tcggatcgtt ccagagaaca gaactgcttt ttgaggaaca
     2641 aagcccatta attctcgcaa attatattga gtcatgtcac taatgtccgt tccatttatt
     2701 tggatcgaac cagattcgat gtcatataat cgagggatca aattgactaa ggtcgtttta
     2761 cctgaaccag tcccccctat gattgcgacg atctcgcccg acttggcttg gaaatcaata
     2821 tcttccaatg caagtttttc tgctccatga tatcgataat tgacatgatt gaaggccaat
     2881 gttgcgtttt ttccagcaaa agaaactgtt ttaggatttt cagggtcatg gataccgatt
     2941 ttttcatcca acacttcatt aatacggtca gctgaagctt gtgcacgagg caccatgata
     3001 aaaatagcgg aaagcatcat aaagctgatc aagatctgca tcgcataagt cataaatgca
     3061 atcagattac cgacttccaa agtcatatct gctatgtagt gtccaccaaa ccaagtgatt
     3121 gctacgtttg taccgctcat gatcaatgtc atcattggca tcatcaatgc gacgatcgta
     3181 ttaactttga ttgccgtatt tgtataatct ttattcgcta aatcaaaacg attttcttca
     3241 aagcgtgtct tatcgaatgc tcgaatcact ctgacccctg tcaatccttc tcgaaatacc
     3301 aggtttaaac ggtcagtttt cttttgcata cttttgaata atggaacagc aaagtacatg
     3361 attccgccga tcaaaataat cattactggg atgacaacta agaagatttt tgtcaactga
     3421 tgatcttttt gataagcaag tatgctcgcg ccaatcaacg tgattggtgc attgatcatc
     3481 atacgtaaaa acatttgtgt aaccatttgg atttgagtca catcattggt cgttcttgtg
     3541 atcaaagagg ccgttccata tttatcaaac gcatcttttg tcagactctc tgatttttta
     3601 tagacatccg tacgcaattg cttacccagt ttctgtgatt cacgagtagc aaaaaatgta
     3661 tttgcgatag cagctaagat actgatcaaa gaaaagccga tcatcacaaa acctgtatgc
     3721 caaatataat cgacatctcc ttgcgctacc cctttatcaa taatattgga ggtaagtgtc
     3781 ggcaaatata aatctgccag tacttggatc accatgaaaa ctgcagctgc aattgctgaa
     3841 gtcaaggaga tccgttttac gatttttagc atctagttcc cttctttcta tttatatact
     3901 ccgtttttca gccaagaaag tttcaaattg aattcactga ttgcttgctc tactgagtaa
     3961 tcttcagatg ttgaaaataa gcgatacgaa tgcgcaattg ttgtaaatac agtattcatc
     4021 actaattgga tcagtaattc caattcatga tcatttttta ctttcagctt cgtccgatcg
     4081 atcaattcaa gcaactcttt tgcatcttgt ttgtgttctt tatgtggatc gtgaggcgtt
     4141 ttttggcgac tcgtatgagg agcgactttt cttgaggcac ggtaatccat gttcataaac
     4201 aagttacgat aaaaagaagc attctcgcct tccaaaatct cataaatcat tttagaaaaa
     4261 taggattcga atcctttgaa tagatcacca tcggattctt tcattagttg taacatatct
     4321 ttagagctgt cacgtctcat tgtctcaaag taataaaaat aaagatcctc tttatcttca
     4381 aaatactgat agaaactgcc tctagggata tccgctaatt tgacgatatt agcaatcgaa
     4441 gcttctttta agggtactct agaaaactcg attctcgcag cctcgattag tcttttttgt
     4501 ttttctttag gcaaatggaa aaatgtctgt gtcggcatat ggatcctcct ttctttcaag
     4561 atgacacatt gtcatatgac gctgtgtcat tataacacct cctacctgtt ttgcaagaca
     4621 tttgcatagt ttttttcatt atcatccgat acattaaaaa agtgataaga ggaatccttc
     4681 catttgaatt aatgagagtt gcccattaaa atagccacat actaaacaaa ggatgagata
     4741 aatggcccaa tcaattagtg tccaagactt caataaattg cctctcgatg ctgacagcat
     4801 cgttttagat acaagaaata ccttagatta cacaaaaaac cacttaagcc actcgctttc
     4861 tatccccgct gctatcttgc caagacaatt aaaaaatcta gatccaaaga aactttatta
     4921 tattctaagc tatacaggca ggcgatcaga agcattagcc gatcaactta ctgctaatgg
     4981 ttttcgtgct gtttatatca ttggtgggat ggaagctctt cagtcgagtg ttgcttgaat
     5041 attgcttgtt agattcaaaa gaggctgtga caaaaaatcg tgtcacagcc tcttttatac
     5101 cctgtctttt tttagttatt tattcttcaa ccttatcaat atgacttgat agccttttct
     5161 ttttctcata gttataacaa tcatagcaat aaaaggagaa ggtaagcata tggcatctgt
     5221 tttagtagtc aaaggtcatc ccctaactgc agaagaatcg cgcacagtta aggcattgac
     5281 gtctttttta acaagttata aagaaaatca tccagatgat gaagtgactg ttttggaact
     5341 ttatcgcgat gatattccgg aaatcgatga agaattatta tcaggttggg aagcattacg
     5401 tgctggtgca gaattcactt ccttatcaga aagtcaacaa caaaaaattg ctcgtttcaa
     5461 tgaattgact gatcagttcc ttgctgctga taaaatcgtt atcgcaaacg ccttatggaa
     5521 tctaaatatc ccaacaaaat taaaagcttg gttcgatacc gtcaatgtag ctggaaaaac
     5581 gttccgctat acagaaaacg ggccagaagg attagtgaca ggtaaaaaag cactgcacat
     5641 ccaatctaat ggcggtgtat acaacggaca agactttgcc tctcaatatg taaaaggtat
     5701 tttaaacttt gtaggcatcg atcaagtaga tcagctattc atcgaaggaa tcgattatga
     5761 ccctgaccgt gcagatgagt tgatgcaaaa tgcattagat aaagctgctg ctttaggtaa
     5821 atcattttaa aaattgagat aaatgcatac aaaagagaca gaggaaaaaa tcttcaaagc
     5881 agcatttgta ccttggatat tttcccatct gtctcttttt gttattcttt ctgctttttc
     5941 aacggatttt cttcataaag agaagagatt gttgtgtacc attcaaaaac atgagtaatc
     6001 actactttag ctgctgcata aacaggaatc cccaagatca caccgatgat tccgaacaat
     6061 ttcccagaag tcagcagaac taataaaatc gttactggat ggattgctaa ctgattccct
     6121 agtactaatg gagagatcaa gcgtccttct attgtctgct ctactgcaaa aacgatcagt
     6181 actttgatga tcatgaatgg acctgctaca atggctaaaa agatagccgg aatcatcgct
     6241 aaaaatgatc ctaagtaagg gatcaggttc aaaaaacccg caataatccc caacgtgaca
     6301 gcatactcta aaccaatgac cgcaaaacca atcataaaca tgattgccac agcaaaagcc
     6361 acagttagtt gtcctcttat ataagaagaa acttgactat tcatttcact cagcacattc
     6421 aacgtcggtt tttgcatgcg tgtcggtaaa aatttgacaa aataaggtgc taattgttta
     6481 ccgtctttca atagataaaa taagataaac ggcatcgtca gcagagccac taaaatagtc
     6541 gctaccgcgc cgacaaaact tccaagactc tgaactgtac tcttagatat atcttggatc
     6601 atatcgcctg ccgaattcat gaacttatca cctgctgttt ccaactgttc gcgaaattgg
     6661 ttgaacagcg ggtcccgcaa aatctcggtt aatttattat caatcgtatc tacatattga
     6721 gggaagttac tgatgaagct cactgtttgt tcctgaatct tagggatgat cactacgctc
     6781 ccccatgcga ttagtgcaac aaccaaaaca aacagcccaa tgatactgta aagccttggt
     6841 acttttcgct tttcaaagta atcaactacc ggattcatca agtaatacaa gattcctgct
     6901 aaaatgatag gcagacctac gattgctaaa aattgccaga ttggtgcaaa caaatatgaa
     6961 acttttgtaa ataaaaagac gatcagcaat attaacaata caacaagtaa ggctgtgact
     7021 acttgattat ttaaaaacca gcgccaaaac cacgataccg gtctgttctt cttatttttt
     7081 ccttccataa ttcctcctgt tagttataaa aaatttggct tcccaaacca atagcaggca
     7141 gtctatgcgg atgaagatag attccatttg caatttgtcc ttcttctgga caagcatcaa
     7201 aaatcaatga tacgtgagct actgtctcta aattcccatt ggccatcgga ccgacataat
     7261 cgatcgtata tacctgatca tcaaaagcaa ttgttccgcc tgctttcaaa gaaaaaggtt
     7321 cgtcttttga tacttcttga atcaccgaat attttcgtaa ggcagctgtt gctgttttat
     7381 cgaacaaaat cagcatgggt tcttccttgt ttattgcttc tgtcccaatt tctgtaatct
     7441 ttgcctgaat cattgcactc tctcctttaa ttgctttcaa aaacagtata acacattctc
     7501 attctttagc cgtgtggaat aattatgtga tagaataaaa acgaaaacta tttcattaca
     7561 attaggagga atcagctcat atgatcgaaa agataatttt aggaacctat acgcgtcgcg
     7621 aaagtaaagg aatctatacg atcgaccttg atacagaaaa agaagaatta gccaaccttt
     7681 cattagctgt tgaagaaaat agccctacct atgtagcaaa aagtaaagct ggcaatcttt
     7741 acacagttac tagtgtggat ggattaggtg gtgccggtgc gtacgataag gattttcatt
     7801 ttttgaatgc ggtaacagag tctggcgctc ctttgtgtta tgtagcggtt gatgaagcac
     7861 gtcaattact atacggggcg aactatcata aaggtgaagt aaatgtctac catatattag
     7921 gagatggcga actgaaagca gcagattcaa tattccatca agaagcgaca ggtcctcata
     7981 aaaaccaaga tcatgcgcat gtccactata cggatctgac tcctgaccaa cgattagtcg
     8041 tatgtgatct tggtacagat cgtgtgtata cttatgatgt gtccgaaaac ggcaaattga
     8101 atcttgtaac taccttcact gcagagccag gaacaggccc tcgccatctt gtctttcatc
     8161 caaatggaca attcgcttat ttatttggtg aattagacag cacagtccgc gtgctttctt
     8221 ataataaaga aacaggtgct tttgaagaat tacagaaaat atcgaccttg ccagaagaat
     8281 tcgacggtga aaatggaggg gcagctatcc gcatttcaaa tgatggcaag ttcctttatg
     8341 cttctaaccg aggacataac tcgattgctg tattcgcagt ttttgaagat ggttcccaca
     8401 ttcaaaacaa gcaaatcatc tcaacagaag gggattttcc ccgcgatttc gcactagatc
     8461 caagcaataa ttttgtcgtg tgtgccaatc agaacaccga taatctgaca ttatatcgcc
     8521 gtcatcctga aacaggatta ctggaattac tgaaaaaaga tgtctatgta ccagaatgtg
     8581 tctgtgttta ttttgatcgt tagtaagaaa aggagaatga aatgaatcag caagacgtat
     8641 tagacagact acgggaagaa ctaaatattc ctttctttga tgggaaaatc gaagataaag
     8701 agtatagcga agaggaatat cagaaactca aagccgattt acagaactac tttgaacaat
     8761 atgtacagaa tgtagaaaac taatcagctt tccatactat ctgaaaagaa catcaggaca
     8821 agacttttcc gtctatcctg atgttctttt ctacaaataa aaaaccgccg agatggttag
     8881 actcgacgga aaaggagtat ttactcgatt ttgagtaagt acaaggttgt tgttggttat
     8941 aggtatgtca ttatcataaa ggattttgat cactctatca taaaggtaat attacattta
     9001 tattagataa ctctagtttt tattgttatc aaagatttct tttatttttt caacaacaaa
     9061 ttagtcattt tttggatggc agctgtcaat tggtctgtcg cttggtcttg ttttgcttca
     9121 gatgtattga atagatcttc atctacccct gattcaagat cgatactttg gatcatttct
     9181 tcacagcctg ctacatatga aagataagct ttttcaaaat ttttatgaat gcccattgct
     9241 tttgctggtg gacgcaaagt actgactttc ttaagcattg cccgatattt ttttgttcct
     9301 tcttcaaata acaggatgat ctcttcaaga gtcgatttgt ccagttcact tgtttttcct
     9361 tcatcaattg cgtttctgat cacttcatat ttcggatgca ttccttctcc gattgcttct
     9421 gtttccttga cgatttgatc gatggactgc acataatagc caatattcgg taacatgttc
     9481 gttttcctac tttctccatt gttgttatta tttattctac ttgtttttat cctcaaatga
     9541 caagtacttg aaaaaattta atggtttcta tacagctcgc cattcttatt cgtgctcaaa
     9601 gaaaccatct gctaaactat attgtttccc tgcgacaatg tcatattgga tcaattgata
     9661 ttcgtcgtaa atctcttgtt gcgagcgatt gaattgcgct tctttatacc actgtccgtt
     9721 ttgatagtac agttctcgtt caaacgccgg aagttcctgt tctaatgata aaagcagctg
     9781 ataaaagcca tttgttgtct gattcgtctg ttccattaga ttcgctgcaa aataaaacgg
     9841 actggtgatt gcatcgtgag tcgtttgctt ttctaattct cctttactgt cgaacataag
     9901 aaattgtgtt tcatgcaatg tttgtttgtc attttttgct tggatcgtat ctgaatagat
     9961 ccctggaaga tgatcccccc aaaaaacaac taaagtcctt ctagaaagat tcttcaattc
    10021 ttccgtgaac tgcttcagtg ccgtgctgct ataggaaata tcttgcagat aattctctaa
    10081 gtcaagtgtc ccacttccat ctgaagtttt tgcaggatag tctagattat catactttcc
    10141 atcgtagggc atatgtgtct gcatcgtgac taaatgaatg aactgaggtg ttttttcttc
    10201 cttgagaagt gtcattactt ctttgtaagc tgactcatca gaaatatatg gattattctc
    10261 tatcgtatcc gtataggtca tcgtacgctc gctgatgaac tggtcaaatc ctaatgtctg
    10321 ataaacatcc tctcttttat acatcgaagt attgtatgga tggatcgctg tcgtctggta
    10381 tgattgtgcg tttaacgctg aaacgataga aggaagctga tccatttttg gtacaagcat
    10441 cgtgtaaggg gtggtgagtt gcgcattaaa taacgccata gaaaaaccag tcaatgcttc
    10501 aaattcgata tttgctgttc caccgccata gttttgagaa agcatattgc cactatatgt
    10561 ttgatcagct acttcataat aatctgccaa tggctcacca gatacttcta cgccgtttaa
    10621 tcgactagga tctgagaaac tttcactcat cacgaacacg atgttcggcg actcttcctc
    10681 tacagtttta tttttttcat cggctagctt ctggtatttt tcagtgattt ctttgatctt
    10741 ttctttcgaa tatccttcag gttcatccat tggttctacc ttcagattgt acaaaaaccc
    10801 accgataaaa ccagtgttat agtagttcat tttctggctg taaggtatcc atagtgccgt
    10861 tttattatat gcttttcgca acaaattatc gggattgttg aaattactga tataccctag
    10921 cagtccaatt gccacaagca gtgtcgttaa tcggatcaat tggaatcgcc ttcctttttt
    10981 caggcttcga aacatgtacc agcaaaacaa tcctatcacg gttcctgcgg cagctaagat
    11041 cactgtaaac agcatagtgc cggtcatatc tttcagcaat ccgatttcag tgatcatctt
    11101 caaatcgtct ggataaatcg gttcttgccg ataactcatt ttcatataat ttgcaaaacc
    11161 taatacaccg atggctacga catacgtaag ggaacctaaa gggacagatc ctgctagtgc
    11221 aatcagaaaa ataagaataa tcagcaagac gagacaggct aagaagaatt tttccgtatg
    11281 ccaagaaaaa gcaaatttca acgcaaggtc tacggataga tcattttgac accactgcaa
    11341 aaataaatta ctaaaaacaa caagaaaaaa gatactgatg attgctgctg ttccttttaa
    11401 aatctttttc tgtttggtcg aaagtctgct tatatatctt ttcaaatgct tgctccttca
    11461 tttctatcaa taaaagaatg ttgcttctct tattttagtt gtacgaaatt cgaaagtaaa
    11521 gaacggaaag aagaattaat gaaatccaaa tattctcttt ctttatggcc tttctggttt
    11581 ctttaataag cggacctttc gtactccaga aattttttct agctgttgtt tcacttgatt
    11641 gagctgttca aaatcagtac tgtctatgtc aatcaatatg tagacatgct cattcttatg
    11701 gttacgcgat atatctgcga tcgctagctc gttctcattc agcagagaaa atatctctgc
    11761 taatatattg gttttctttt gataaaatat agtgaatcga aacggcgctt ggaaaaacat
    11821 tcgtgcggat ggataattga ccgcttctct aaccgtaccg aacaaaagat aatcttttaa
    11881 ggcttcaact gccaaacgtc cgctgtctgc caaagcttcc tgagtcgatc cccctagatg
    11941 aggcaacata agtatacggt cattttctaa gaactcttct tctggaaaat cagtcaagta
    12001 tttagccaga tgttgttttt ccaatgcttg cataacagca tatttatcaa caatttcact
    12061 tcttcctaca ttgataagta cagcatcttt tttcattttt tctatattcg cctgatcgat
    12121 caatcctttt gtatcttctg tcaacggcag catgactacg atgtaatccg aggtagaaag
    12181 aactgtttct aaatctgctt gccgaacata atccaactgg gcatcgcgga tagaataacc
    12241 caagacatcc atccctaagc tatagcaact tagcgctact tttgtgccta tcgctccaag
    12301 accaagaaga ccgatggttt tgccttgcaa ttctcgtccc acataagcac ttcttttgtt
    12361 ttctgcctgt tctaaaatat taggacctgt tagtgtttgt accatacgac ttgcttcgat
    12421 tatcggacgg gaagataaga gcaaacagca caaaacaagc tcttttactg cattagcatt
    12481 tacacctggt gtattcatga cgatcgtccc gttttcagaa gctttttcaa cgttgatcgt
    12541 gttcacccca acaccagctc ttgaaattgc tagcaaatct ggtgtaagcc attcatcttt
    12601 cactttcgta ctacgaataa ctattccttc ggctggcaaa tcatctatta ttttgaaata
    12661 ttccccttgt aacttttcta tctcttgttc tggaatagca tcaatcaaat gaacgtgata
    12721 cataaaaccc cctccttact aacaagtatc tcgaaatttc tgcaattaga caactgttta
    12781 ccatttgact tcctagaagc aaaaagagca gttagagcag agtaggctga gtgataaaaa
    12841 taaggctatg acaagttact gccataagcc ttattttcga gagcaaaata ttgagggtga
    12901 gatatcttga taaatggtgt tccgttagct gacattgata ataagactaa atttcaaaaa
    12961 agttgaaaag caaatttcgt caatctcttc ttatttctta aaactaagtg gctttttcac
    13021 aacctctttt tatctaataa ccactgtttc caactcaact aattttgccc acgtttcaat
    13081 ttgatctgtt gttagattta gcgagacaac ggtatgatga ccgccacctg cctgaatcca
    13141 tgaatggaca ccttcatgga aattcggttt agttttccat aacacacgtg caacaggtaa
    13201 attaggtgct tcagtcgttg gttcaaaggc ttctacttca ttgatcagca gtttataatg
    13261 cgtaccgaaa tctgccatcg atacgactac cccttctcct gctttcccat caaatacaag
    13321 acgcgccgga tcttctctat ctcccattga taaaggagaa acaacgattc ttggtttcgt
    13381 cgcagcaagc gtcggatcta cttccatcat atgggattcc aagattgctt cttggccact
    13441 agctagctcg taagtataat cttccatgaa tcctgtatct ttccctcgag ccatgatttt
    13501 cagcaaccga tcaatcgctg cagttttcca gtcgccttcc ccagcaaaac cgtatccttc
    13561 tgccatcaaa cgttgtacag ctagtccagg tagttgtttc attccataca ggtcttcaaa
    13621 attagtcgta aaagcgttat agcctccttc ttctaagaaa cggcgaatac cgatttcctg
    13681 ctgagcttgt acttttacat gttcctccca tgtcttttct tcataatcac catagtcgaa
    13741 ggtatacaat tccttgtact cttcaaataa ttgatcgact tcttcgtctg atacgcgatc
    13801 gattacttgt acaagatctc cgataccaaa ataatcaaca gtccaaccaa attgaatctg
    13861 tgcttccact ttgtctcctt ctgtgacacc cacgtttctc atgttgtcac caaaacgagc
    13921 cacttttatt ccaaaacttt cattataggc tactgcaaca tccatccaat cagcaatctc
    13981 tttttggatc tcaggtcgtt tccagtagcc aacgacgatt ttattttgtt tatttaatcg
    14041 agcattgatg aacccatatt cgcggtcacc atgagcggat tgatttaaat tcatgaaatc
    14101 catatcgatc gttttccaag gaatgctttc gttatattgc gtagctaaat gaagcaatgg
    14161 tttttgtaga agctttgtcc cgcggatcca catttttgct ggtgagaaag tatgcatcca
    14221 agtgatcaca cctgctactt cttctcgata attgacctct ttcatgattt ttgtgatcgt
    14281 atcagcagta actgctaatt cttgaaggcg gattggataa ggcaattggc cattttcatt
    14341 cagttcatcg accatttctt gtgcatgttt ctttacttct cgcagtgctt cttccccata
    14401 taaatgctgt gatcctacaa caaaccaaaa ttctttttct ccaatattca acataattag
    14461 ccactccttt tatttgtttt gtccgtaata cgcatttttt ccgtgttttc gtaaataatg
    14521 tttatctaaa atccgttgag gcagctcttc tgaaaatgaa ttgagctgtc tggtaaataa
    14581 attcatcttg catacttcat ctagtacgac tgcgttcatt accgcacttt gcgcatcttt
    14641 cccccaagta aatggaccgt gtccgtgtaa aagtacccct ggaattgcta agggatcaat
    14701 tttgcgttct ttgaatgttt ctatgataac ttttcctgtt tcatattcgt acccgctgtc
    14761 gatttcctgt tgtgtcaaaa atcgagcaca aggtaccgat ccataaaaag tatctgcatg
    14821 cgtcgtcccc atcgcaggaa catccaatcc tgcttgtgcc cagatcgtcg cccatgtcga
    14881 gtgagtatgg acgattcctc cgatttgagg aaatgaacga tataaaacag catgagtagg
    14941 cgtatccgaa gagggattga gtttaccttc aatgatatga ttgtccaagt ccacaacgac
    15001 catgtcgtct gctgtcatct cttcataact gactccgcta ggtttgataa cgaaatatcg
    15061 tgtttcagga tcaaaagcgc tcacatttcc ccatgtatat ttgacgagtc cgtgtttagg
    15121 caagtcaaga tttgcttggt atacttcttc tttcagttgt tccagcattt tctatcactc
    15181 ctttttcttg caattgatga atcgctgttt tttcaatgtc gatcccctca cggtaacgtc
    15241 tcataaatat ctgatagcct gctatttctt ctgctgaagg tgtgatggtg attccttggt
    15301 cttgtccaaa cacgtcttct gccaagaagt tttctaaaga cttcccatgt cgatccatca
    15361 tataggctgc taataacgcg attccccacg ctccaccttc tccggctgtg tccatcactg
    15421 taactggtgc ctccatagca gatgctagaa tccgctgtcc aacttctggt gttttaaaaa
    15481 tccccccatg ccccactaaa cgatcaattt ggacattctc agatttcaat atatccattc
    15541 cgattctcat tgcaccaaaa gcactggcta aatggatccg catgaaattc gctaaatcaa
    15601 aacggctacc tggtgtacgt acaaataatg gccttccttc atttactcct gtaatgtttt
    15661 caccggaaaa ataaccataa gaaagtaatc ccccgcaatc cgtatcgcct tctagcgctt
    15721 tgttgaataa taccgtgaat aatttatctc tgcttatctc catacccaaa gaattggtaa
    15781 attcttcaaa aatactgacc caagcattga tatccgaaga gcagttattc gtatgaacca
    15841 tgcctactag ttctcctgaa ggtgtagtga ccatgtcgat ttccggatgg acattttcta
    15901 gttctttttc taatacgatc atagcgaagg cggaagtacc agccgaaatg ttccctgtcc
    15961 gtttggctac actgttcgta gcgaccatac cggttcctgc atccccttct ggcggacaaa
    16021 ccgggattcc tggtctcaaa tttcccgtag ggtcgatcag ataagcccct gattcgctta
    16081 acttgccagc ttcttctcct gctactagaa ctttaggcaa aatgtgatcc aagtcaatcg
    16141 ctacgtgcgc tttttcgatt aactgaccaa aaatattttt catttcttga tggtaggttt
    16201 tcgttgaagg atcgatagga aacatgcctg atgcatctcc tacgcctagt actttctcac
    16261 cagtaagctg ccaatggaca taacctgcaa gcgttgtcaa aaaattaatt tctttcacat
    16321 gttcttcttt attcaaaata gattgataca aatgagcgat actccatctc tgcggaatat
    16381 tgtattgaaa tttttccgtt aatattcgtt ctgcttcttc agtgattgta tttcgccaag
    16441 tacgaaatgg tactagtaac tgttccttgc tatcaaatgc tagataaccg tgcatcattg
    16501 cactgaaccc aatcgacccg attgtcgtaa gattctcgcc atagatttcg aatacttctg
    16561 ctgccatttt tcggtaactg acgcgcagtc ctttccaaat atcttctaat gaataggtcc
    16621 agataccatt ttctagtttg ttttcccact cataactacc agcggcaata ggagtaaaag
    16681 atgaatcaat caatacagct ttgatcctag ttgatccaaa ctcaatccct aaagatgtac
    16741 gaccatctat gacatctaac atactttgcg catttacttc cttcataatg atttaactcc
    16801 ctccttctcg tcaaacctga tcaaaccgtc tttttagtag cgcttacaat gattattgta
    16861 tacttttgta cgtacatgtc aagcggttat ttacaagaat acaataaaaa acacttgttt
    16921 tgtacgtaca taagttataa aatgaaattg cttacaaaat ctgatcaacc gccttctcga
    16981 aattctagaa ggagagaata cttatgaaaa aagaaaaaaa agtttcctca tcgtttattt
    17041 atttttttgg cgcatttggc ggcattctat ttggatatga tattggtgtt atgaccggcg
    17101 ccttaccttt tttacagcat gattggggtt tggcaggtaa agcttctttg ataggatgga
    17161 tcacgtcttc ggtcatgtta ggtgcgatcc ttggcggctc tttatctggt cttctttctg
    17221 ataagcttgg acgacgaaaa atgattcttc tttctgcact tatttttatg gctggttctg
    17281 tcctttctgc atctgcccct cacaatggtt cttattttct gattgcagca aggattcttc
    17341 taggtttagc tgttggcgct gcatctgccc tagttcctgc ttatatgtct gaaatggctc
    17401 ctgctagact aaggggaaga ttgtcaggga tcaatcaagt gatgattgct tctggcatgc
    17461 tcctttccta tgttgcagat tatttgctaa aagtgttgcc tgaaacgatg gcttggcgcg
    17521 tgatgttagg gctcgctgct gtccccgcgc tgattttatt tttcggtgtt ttagctctac
    17581 cagagtcacc gcgttttctt atgcaatctg gccgcttgga agaggcaaaa agagtattga
    17641 actatattcg gacaccaaaa gaagctgaac aggaattcga acaaatccag ctgaatgtca
    17701 aacaagaaaa gacaaccgga acttcttggc atactttgtt tttagaaaaa tatcgttcct
    17761 tagtcattgc cggtattggt gtagcagtat tccagcaatt tcaaggagct aatgctattt
    17821 tttactatat tccgctgatc gtagaaaaag cgactgggca tgcagcgagc gatgcattga
    17881 tgtggccgat catccaagga atcattttag ttgctggttc tttgctcttt ttggttattg
    17941 cagacaagtt caatcggcga actttattga aaataggcgg tagcgtgatg ggactttcat
    18001 tcatcctacc agctgtttta ggaactgtac tggatgctca tacaaattcg ttgttgattc
    18061 tcttgttttt atgtatttat gtcgcatttt attcttgcac atgggctcca ctgacatggg
    18121 taatcatagg cgaaatattc ccactagctg tccgcgggcg agcatctggc ctagcctctt
    18181 cttttaattg gattggttct ttcctagttg gcttgctttt tcctgtgatg actgcttcta
    18241 tgtctcaaga aatcgttttc ggcatttttg ggatcatctg ctttttaggg gttttattta
    18301 ttcaagagat cgtaccagaa actcgtggga aatctttaga ggaaatcgaa caaagtgcat
    18361 ctaaaaaaac ttatcctaaa cgtatttcta tccatcattc ttaaagaggt tgtgacagaa
    18421 gtggtcagct tcaagaaata agaaggaacc gcttagtttc cagtcacaag gaacaatttc
    18481 aaaaaacagc ttctcatgtt tgatgaatat tgtgacttgt gaccagttgg taggagaaca
    18541 ccgtttagtg gagaggtggt gaaagaaacg ttcagccttg agcgatgaca aaagctctca
    18601 caaacattgc gacgagtaac cgtaggagca ataagaggcg gtaaaaaccc gtctcttatt
    18661 tttcgttttc tttcactgtt tcccgttcaa ctagtacagg ttcatagaga atatttggct
    18721 gtgcctgtcc tttttcaatc gttgtgacga tccaccgtgc ggcatcttgt cccaactttt
    18781 cttttggatg cgtcaatgtt gtcaattttt tattccctaa atggcttaaa gaagaatcgt
    18841 ctgttcctac gatcgatagg tcatctggaa tatgatagcc attttctgtc aacttatcta
    18901 gcattaaact agctatttga tcgttatagc aaatgatgcc atctaaagct tgttcttctt
    18961 tcaaccgttg tactgttttt tcagcgattg tttctcgtgt ttccgtggtg aaagtaatga
    19021 tatcttccgt caaaaaacgc acattatact gttcacacgc gctgataaaa cctttcatcc
    19081 tgtattttcc ttgaagatcg tcgatttttg tgatgaacaa cagacgctga tgtccttttt
    19141 ttatcaaata ttctgctgcc ataaaaccga ctttcacatc atctaaacaa atataagagg
    19201 catttaactc ttcataagca gcattgatca tcaccatcgg aatgccttgt tctctcaaag
    19261 caacataaag tgctaagttt gggttatact gattactttt cgtaggctca acgatcaaac
    19321 catctacccc ataagcaatc attttcttca aacactcttt ctccaattcg tgatcgttat
    19381 tcgtactggc taaaagcaat gaatacccag attctcgtaa ttcattttcg attcctcgga
    19441 taatcgacgg aaaaatataa tcagagagat atgtggtgat gacaccgatc gtttttgttc
    19501 tgttcaatgg tgttttttct ttcttatagc tgtcatctac atatgtgccc gacccttttt
    19561 ctttacgcag atagccctca tttaccaaca cagcgattgc ttgccgaatc gtatgtctgc
    19621 tgacgtcata tatttcttgc agcttgaatt ctgagggaat ggttgtattt acttcgtatt
    19681 cctcacgaag gatcttttgt tttatctctt ctgcaatatc ttgatatttt gccatttgcc
    19741 ttgccccttt ttatttgtac gtacatatat cataacgagt ctgcaaggtt tattcaagca
    19801 aaaatcaagg tttcttgtgc tcataaggca tacggaacag gttcaccaaa ttctggaaat
    19861 ccttgctcat tccacgtaaa gcattgcgcg ttcgcgtgcc gattaggatt gtctaacgga
    19921 tccccttttt ggttcttctc tggtcgagca tgatagatca aaatatcaaa gcacccgtct
    19981 tctgaaaccg taaacgagtt gtgaccagga ccaaattgct tatttttttc agaagaaacg
    20041 aatactggct ccgataattt atgccatgat tcttttaata aaagattctt gtgctcatca
    20101 gcccacagca ggcccatggc atagttttca tctgttgcac taccagaata cgtaatgaat
    20161 attttcccat tgcgagtaag gactgctgga ccttcattta ctgaaaaacc ccgtttttcc
    20221 cattcatact caggtataga aaggaggatc tgcttcccct ttaacgtcca aggattatcc
    20281 atttcggata gataaagatt agaattccca gtaatttcag gatcttgttg ggcccacaca
    20341 taatatagct ttccttgaga agagaaagtt gttccgtcta aactgaaagt ttcccttttc
    20401 gtttttattt gtcccttttc tatccaatct gtattcattg gatctatatg ctgattttca
    20461 agtacgaaca tgcggtgatg atgagattca tcacgtatag ttttatgatt gcttgcagca
    20521 aagtaaatat accattttcc attgatataa tggacttcag gtgcccagat caattcactc
    20581 atcggtcctt cttcatgagc ctgccataca atcaggcttg cagcatgttc taattcattc
    20641 agtgactttg cccttcttaa ttcaatcact tggtagcctg gaaccgatcc tgtaaaataa
    20701 taataaccat ctgtatgttt atatacccaa ggatctgccc gttgccgaac gataggatta
    20761 ttataaagtg tcttcatcat aacaaatttc ctcacatttt ctttattcta attacattcc
    20821 aagagtattt ttctatccct acatttaatt cgtcatgttt ttctattgaa aaatagttta
    20881 tttcaggttt tattttttct ggttcttcac gtgtattttt gtccaacaga tcgctactgg
    20941 ttaatgtgta gtgctctcct tgatctagca aattgaaatc atgttcaatc gttatctttt
    21001 gttgttcatt tgtgcgattg atcaagaaaa tagtcagctg attgccgtta tctacgatca
    21061 cactgtccac ataaggaaca ttttttatct tttcacaatc ataaacaggg caaatgattt
    21121 gttgtttcaa tacttgacct ttagcatatt tcgataagtg ttgcatcaca taataagttg
    21181 gattcctcca tgcttgtcct ttagcatctg tcagtattaa aggtattgtg tttactaaca
    21241 atgattgaca actaatcttt acgcgatctg cgtgtcgtaa aatagccagc cctaatgaac
    21301 caaataataa tgtatcttcc atagtaaagt aacaccaatc gatgggacta ccgacttcaa
    21361 aatccctatg tcttttttcc ggttgggcat gtacattcca ttcatcaaac gctagataca
    21421 ttgttttctc acttctcaaa tatcctttta cataatcaca agtagcaacg atttcttcaa
    21481 tttgacgatc gaatcgataa gcttttgcaa ggtatgtcgc tgtatcatca cgctcatttt
    21541 taggaggttt agtcaagtct tcttcatcat acttatcaat atagttatgc aaagctaaat
    21601 aatctacatt ctcataagct tcttctaatg cgatacgatc ccattcaggg tacgatggta
    21661 aacgtggcgt agagcttcca actaagattg tttcaattga atcatccact aacttcatga
    21721 cttttgaggc ttcatttgct aacctaccat attcataagc actcttagcc cccacttgcc
    21781 atggaccgtc tagctcattc cctaaacacc ataatttaac gccaaaaggc tttttttgcc
    21841 cattttttat tcttaaatca ctccagaagg ttccttttgg aaaattacaa tattctacta
    21901 aattttttgc ttcatcaatt ccttttgttc ctaaattcac cgtgaatatc ggtttcgctt
    21961 gtacatgact gatccattta aaaaattcgt ggagtccaaa agaatttggt tctatacctc
    22021 gccatgctaa atcaattttt tttggtcgtt gttctttcct acctacagta tccacccaat
    22081 cataaccaga tgtaaagttt cctccaggat agcgaatgac acctaattct aattccttta
    22141 cataagagag gacatcttgt ctaaacccgt caccatcagc tgtttcatgg cccggttcat
    22201 aaatccctgt atagacgaca cttcccatat gctcaacaaa tgagccaaac agccgagaac
    22261 tgatctctcc taccgtaaaa tcctctctga tttttacttt cacttcttcg tccaacagct
    22321 ttccctacct ttcatttcct atagtcaaga taatagatac aagtttccat acgccaccag
    22381 caaagataaa aataaaagga gggaaaagaa atatgagcag tacaaatcct aataacaata
    22441 cgcaacaaag aatatttctc aaaaaatcaa aaaatacttt ccatgcactt tgttcaaaaa
    22501 tttgtaaaaa tgtgatatct ttgttttcag aaatcacata tatccaggat aaaagaaaca
    22561 tggaataaat aagtaaaact acatatccac taattgaaag tacgattgtc tgttcttcta
    22621 ataattgatt aacaagaaaa taaataatga tcgttaacag actaaggaac aagttcttac
    22681 caaaatgttg ctttattcct tgaaagaaaa aataaatgat atttcctgat ccctcacctt
    22741 tccattgcgc cattacataa aaaacagcac atgtagcagg cacgatagta ataacaggca
    22801 aacacacgat tacccagaga atacctaata agatgtaatc agaaaccaca atcaactgtc
    22861 cataaaattt agtctcaact attttcatct tcctcactct ttcattccag acatagctgt
    22921 tccttgaaca aaatatttct gtgcaaaaat ataaaccact agtaatggca gcattgaaat
    22981 aaccgttcca gccattacag gtccataaaa agaaatgttc tgccctgtaa ataaagctaa
    23041 ccctgctggt aaagttctca tttttgaact acttgtcaat atcaatggat aaagcaagtc
    23101 attccaacta ttcatcagcg tgaaaatccc taatgataaa agagtaggtt tgctcaatgg
    23161 caacatgatt ttcaaaaaaa tattaaactc acttaatcca tcaatgcgtg ctgcctcttc
    23221 caaagaatct ggtaaagtaa taaaaaagga acgcatcatg aagataccaa atgcagttgt
    23281 catacgagga atgatcaaac ctgcataagt atcaagcatg ccaagcaaat tgacttcaat
    23341 aaatagagga atcatgaaga cttggaatgg gatcatcatt gtcagcaaaa cgaaatagaa
    23401 caaaactgat tttcctttga aacgcatacg agcaaatgca taccccgcaa gagaatcaaa
    23461 gaatacagaa gtgatcactg tccctccagc aaaaatcact gtatttttga tgtaatctaa
    23521 taaagggatc gttgtccaaa ccttaatata gttatctagg gtatagctat ctgcaaatat
    23581 tctaggcgga aatgagataa tgtctttttc gtgtttaaaa gaagaaataa tcagccacaa
    23641 aaaaggaaac gcaataacca acaatataac tagcatacta atcgttcgaa tgattttttt
    23701 accactaact gcttttcgac tattcacagt aactgtaatc gagctttctg gtgccataca
    23761 ggtttccctc ctatttcatt tgtttttctt tccgtgacat gtacacatta gtggagatag
    23821 ctatagttgc aacaatgata aacaaaaccg aagacagtgc tgatgcatag cccaattcat
    23881 aaggtgctgt aaatcctcga ctgtaaatat attgaacagc tgtttctgtc ttaaattgtg
    23941 ggccaccagc ggttgcaaca tatacttggt caaacacttg catcgaacca atcagattcg
    24001 ttaaaataca aaaacctaag gagggaacga tctgaggtag cgtaatgtta aaaaactgct
    24061 gtcgatttgt tgcaccatcc atttgagcag attcatacaa agagggagag attccctgaa
    24121 tagcagtcaa gagaatcgtc atagtaacac caaaattctt ccaaacagtc atgatggcta
    24181 ccgtcggcat agcaagggtt ggatcgcgaa aaaattgagg gatcggcaag ccgacttgtg
    24241 aaaacaagta aggaatcgcc cctacattcg gatcaagcaa catagaaaaa ataatcgcaa
    24301 ttgacgtcaa tgaacaaata actggaatgt aaaatgtcga acgacaaaaa cgattgaatc
    24361 tggtattttt agataatgct actgctgcta ataaaccaac gataatttgt gttggcgttt
    24421 cgagtacggt gaaatataaa gaatgaatca acgaattaac tgctcgttca tcactaaata
    24481 tacgtgtgaa attttcaaaa cttacaaaat ttcttgatgt aaagaaaata ttcaaatctg
    24541 taaaactaat agcaaatgtt cctaaaagcg gtatgacagt aaataataat aaaactaaaa
    24601 aagatggagt taaaaacaaa tacgcagcac gatcaggacg attccaatat ttggtgaatc
    24661 cgttcatata gtccacctca tctttcattt tgataaaata tacaatcttg gtttaaacga
    24721 atctgaataa gatatccgaa caatcgctgt gatttttgca ataacaccta tctgcaaata
    24781 ctaggtcgat cattcagata tctgtatctt gccaacagtc tatcctgtat aaagttctgt
    24841 actatttttg cagtaattga ttgatggcat catctgcatc gttcatgagt gtctcaggat
    24901 cttctcctgc taataatttt tcaatcagtg gattgatgat atcgttattg atcgcagaga
    24961 tttgggtaaa acctggtaaa tatggctctg catagtccat ttgtttcgat aattcagata
    25021 caataggatc attttttact tcttcatctt ttgctactga atccaagtag gcaggaaaac
    25081 cattttcgat actccatttt ttaccaattt cagttgtatt ccaatatttc aaaaactcat
    25141 agacagcttc ttttttatta ttatctgtac ttgccggaat ggcatatcct acgccatcta
    25201 aaatagctgt ttttttgtct gaatcactcc cttgtggtaa ggtagtcaca ttataatcaa
    25261 tctcgtttgc tttcaatccg ttatttaacc atggcccatt gatttcaatg gctagttgac
    25321 cagcattcat taaattgtcc atttcggcgc cagaaatcga ttctggacca gtcttgtcta
    25381 catggatcat tttttggatc gcttttaacg ttttaaggtt ctcaggagac gcaaattcag
    25441 acttagtata atcatcgttc actaatttac cgccatttgc taacatccag ttatataacg
    25501 gtgcagttcc atccttattg aagacaaaac ctgatacatt tttactacta tcagttaatt
    25561 ttggtgccat ttctgtcaat tcttcccaag tagtaggtgg ttgttcagta tctaatccag
    25621 cttgtttaaa caaatcatta ttccaataca tatacatgcc ttgtacctgc atggggacaa
    25681 aatactgttt accttctact tcacctaatt caatagccgt ttctttaaaa tcagatttat
    25741 ctactccatc atattcccag aaatcgtcta atggttgtac cgctccgttt tctacatact
    25801 gtgcaaaatc tggataattt aatgcaataa agtcaggtgc ttttttagaa gaaatcgctg
    25861 taggaagttt ttcattcaaa ttggcccaag tcatgatatc catttcaatt ttgatgtttt
    25921 tatcatttgt tttattaaat tcattaacga tttcttgcaa tatttcacca tcagatgcag
    25981 taaacccatt ccaaaaagtc aaagtcactt ctttatcaga atcagattct gctgaacttc
    26041 cgccaccgca agctcctaaa atacctgttg cagctaccaa caaagagcca attaaccatt
    26101 ttttaccttt cattttttgt tcctccttca tctgataaac gacatctgac cacattccaa
    26161 gatagcggtt tcaataaaca attgattttc ccatcttcag cttttacatt tgttgaatga
    26221 cttggtttta cttgttcact tttaggaaga ttagttgctt ttgtatcaaa tccactcatt
    26281 tcagaaaaat caatcactcc ctctaatgat aattcagaca agtgaacagt tagattcatt
    26341 tcttcttctc cacggttaac tgcaaatatg acaagttctt tgtcatttat tacggctata
    26401 ctttctaaat acggcacttc ttcaaaatct tccgtcgcat agtttgctga acgaatttgt
    26461 gggcttaaca cctctccttg tccatacaca gcagcttgca taaatggata atagatcgtc
    26521 tgtctccaag cttctccgtt tgtttctgtc ctgattggtg caataacgtt taccaattga
    26581 gcaagacaag caatctttac tctatctgca tgtttcaata aagtgattaa tagacagcct
    26641 aatagtaatg catcttcaaa attatagtta tcttctagta aaggaggagc aatctgccaa
    26701 ggcgctgctt gctcatcttg ttccgctgaa tgataccaaa tattccattc atcaaaggac
    26761 aggttgatct gcttcttact atgtttcttc gccttgactg tatcgcacat cgctgtcact
    26821 tcagaaatga accgatccat gtctaaggaa cgtgcaagat aatgagaaag atcatttttt
    26881 tggttgccat aatattgatg cagagacaaa taatctattt gatcgtacgc ttgatcaaga
    26941 actgttaact cccaatcacc aaatgtaggc atttgccgat tcgaacttcc gcataaaacc
    27001 acctcaattg aatcatctac gagtttcatc gccttagccg tttcctctgc taaccgtcca
    27061 tattcttccg ctgttttatg tccaatctgc caaggaccgt ccatttcatt ccctaaacac
    27121 catgtcttaa ttccaaaagg gctctcttga ccattcgaac gccttaaatc actccaataa
    27181 gttcctttag gaaaattaca atattcgact aaattacgag ctgcatctac tccacgagta
    27241 cccagattga cagccatatt gatttcggcc cccacatgct gacaccagtt ataaaattca
    27301 tgaatcccaa cttcattcgt ttcaattgtc cgccaagcta aatccaacct tctaggtcta
    27361 tttgctatgg gcccaatact gtcttcccaa cgaaatcctg acacaaaatt tccaccagga
    27421 tatctaatca tgggaatgtt caattctttt accaagtgaa tgacgtcttg gcgaaatcca
    27481 cttttattag ctgatggatg atctggctga taaatccctt catatactgc tcgtcccata
    27541 tgttcaataa atgaactgta caaccgcgga tcaactttgc ctacacgctt gacactacta
    27601 atcaacattg tggcttccat aactgacctc ctttaattta tacatgaata atacacctcg
    27661 ttgaaagcgt tg
//
LOCUS       DAZKOW010000028        26868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000028 DAZKOW010000000
VERSION     DAZKOW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.028"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(83..1498)
                     /locus_tag="ACG912_001169"
     CDS             complement(83..1498)
                     /locus_tag="ACG912_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512502.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(1509..2177)
                     /locus_tag="ACG912_001170"
     CDS             complement(1509..2177)
                     /locus_tag="ACG912_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFQ0512503.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2307..2891)
                     /locus_tag="ACG912_001171"
     CDS             complement(2307..2891)
                     /locus_tag="ACG912_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0512504.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3222..3455)
                     /locus_tag="ACG912_001172"
     CDS             complement(3222..3455)
                     /locus_tag="ACG912_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0512505.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3470..4420)
                     /locus_tag="ACG912_001173"
     CDS             complement(3470..4420)
                     /locus_tag="ACG912_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFQ0512506.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4417..5259)
                     /locus_tag="ACG912_001174"
     CDS             complement(4417..5259)
                     /locus_tag="ACG912_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFQ0512507.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5261..5980)
                     /locus_tag="ACG912_001175"
     CDS             complement(5261..5980)
                     /locus_tag="ACG912_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512508.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6528..6818
                     /locus_tag="ACG912_001176"
     CDS             6528..6818
                     /locus_tag="ACG912_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512509.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNT"
     gene            6890..8116
                     /locus_tag="ACG912_001177"
     CDS             6890..8116
                     /locus_tag="ACG912_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0512510.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8204..8596)
                     /gene="rpsI"
                     /locus_tag="ACG912_001178"
     CDS             complement(8204..8596)
                     /gene="rpsI"
                     /locus_tag="ACG912_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0512511.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8612..9040)
                     /gene="rplM"
                     /locus_tag="ACG912_001179"
     CDS             complement(8612..9040)
                     /gene="rplM"
                     /locus_tag="ACG912_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFQ0512512.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9574..10041)
                     /locus_tag="ACG912_001180"
     CDS             complement(9574..10041)
                     /locus_tag="ACG912_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFQ0512513.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10161..11699)
                     /locus_tag="ACG912_001181"
     CDS             complement(10161..11699)
                     /locus_tag="ACG912_001181"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFQ0512514.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11779..12465
                     /locus_tag="ACG912_001182"
     CDS             11779..12465
                     /locus_tag="ACG912_001182"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFQ0512515.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12666..13193)
                     /gene="msrA"
                     /locus_tag="ACG912_001183"
     CDS             complement(12666..13193)
                     /gene="msrA"
                     /locus_tag="ACG912_001183"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFQ0512516.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13277..13954)
                     /gene="pgmB"
                     /locus_tag="ACG912_001184"
     CDS             complement(13277..13954)
                     /gene="pgmB"
                     /locus_tag="ACG912_001184"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFQ0512517.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13951..15081)
                     /locus_tag="ACG912_001185"
     CDS             complement(13951..15081)
                     /locus_tag="ACG912_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0512518.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(15081..17264)
                     /locus_tag="ACG912_001186"
     CDS             complement(15081..17264)
                     /locus_tag="ACG912_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFQ0512519.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17261..18286)
                     /locus_tag="ACG912_001187"
     CDS             complement(17261..18286)
                     /locus_tag="ACG912_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0512520.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18331..19158)
                     /locus_tag="ACG912_001188"
     CDS             complement(18331..19158)
                     /locus_tag="ACG912_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFQ0512521.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19155..20222)
                     /locus_tag="ACG912_001189"
     CDS             complement(19155..20222)
                     /locus_tag="ACG912_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFQ0512522.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20248..21084)
                     /locus_tag="ACG912_001190"
     CDS             complement(20248..21084)
                     /locus_tag="ACG912_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0512523.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(21081..21950)
                     /locus_tag="ACG912_001191"
     CDS             complement(21081..21950)
                     /locus_tag="ACG912_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0512524.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(22020..23303)
                     /locus_tag="ACG912_001192"
     CDS             complement(22020..23303)
                     /locus_tag="ACG912_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0512525.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23317..25002)
                     /locus_tag="ACG912_001193"
     CDS             complement(23317..25002)
                     /locus_tag="ACG912_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0512526.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25277..26302
                     /locus_tag="ACG912_001194"
     CDS             25277..26302
                     /locus_tag="ACG912_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0512527.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT         8072 a         5411 c         4473 g         8912 t
ORIGIN      
        1 aaaaagtaaa ttactaggga aataactgta gctaaaccaa taaaaatata atcttttgtt
       61 ttcatatata ttctcctcaa ttctatttcg ttatgtcatt aaaaatctgt tctatcaaag
      121 ttgaatcttc tactctaagt tttatatctt tttttattcc accatttttt aaataaataa
      181 ttctattgca tgttttataa agaaattccg gatcatgagt agctataact actgcaccct
      241 tttcttttaa gtctttaata aggtcagcaa tcctttccat attaatataa tccaacccac
      301 ttgtaggttc atcaaataga aataaattag tttttgacaa tgaagctata gaaaccaata
      361 atctttgttt ttctccaccg gataaatcaa acggtaattg atctttcatt ttataaagtt
      421 ccattttttt taaaattttt tcttgctgat ctttactaac tatttcattc cccagggcta
      481 actcgctcct cactgattct gtgaaaaatt ggtaatccat ttcttgcatt actaaaaacg
      541 gcgatgacaa atttttttta ttttcgatta tctttccttt ggtagggcga ataatcttca
      601 tgagtattct tagtaaggtt gttttcccca caccgttttt ccctaccaaa ccaataacat
      661 ctccaacttt gacatcaaaa tttatgtctt ttagaatatt ttcataagta atttcttcaa
      721 cttttaatac atctgcactt accttttcga tatagggaac ttcaatcgaa ctattaaaaa
      781 tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa caactgaata gttgtagttt
      841 ttacagttct atttttaaca aatagaactt gatcaattaa ttctttcaaa tagtaaaaac
      901 gatgttcagc tataacaata gtaaatcctt tattttttaa atcacaaagt aagtcagcaa
      961 gtttcttagt cgctttgaag tccaaatttg aagacggttc atccaatata attaatttag
     1021 agtttagcga taaactacat ccaatagaaa ttttctgccg ttcaccactt gatagcgaaa
     1081 aaatgttttt atctaatata tttgaaagat ctaataattc agctaattca ttgattcttt
     1141 tatccatatg ctcttttgta aatccaaagt tttccatcga aaatactaac tcactcgttg
     1201 tatttgttgt aaagaattgt cctctaggat cttgacttac gtagccaata ttttttgata
     1261 tactttcttg tgttaaggat tgtagcgagt tattttcaaa taaaatttcc ccaaccaatt
     1321 ttccgtcata aagtcttgga ataagtccat tcattaactt taatagtgtg gatttcccac
     1381 tcccagaaga accagtaatc aatgtaagac tgcctttttt tattgaaaaa ttaaggtcaa
     1441 caagcgcttg ttccttaaca tccttatatg aaaaagaaac attgttaaat tcaaacaatt
     1501 ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa cggaaaacag gatgatatag
     1561 tctaataagc tcatgtttgt tttatagtaa gaacttcttt tcacaggaga ttctatcccc
     1621 tttgttattg cattagcggt catttcttca gcaatcatag ttgcacgtat aagaacggga
     1681 actaacagaa attccaaata ttgcagaggc tgaaaaaaag acaagcgaat gtttctcatt
     1741 tttaaagatt ctttaatcat tcttatttca gaacgataag tcggaaagaa tctgatcact
     1801 actgtaatac ttaacattat ttttttcggt aaatgtattt tttctaaaga ggataataat
     1861 tcattgctag tatagacact aaccaacaac catgagatca ttacgaccgg caacattcga
     1921 aatacaatat ataaggatgt cgcaaaaaat atagtaaaaa actctatctt gatagcatca
     1981 ataattttca ttactaaaaa cagaactgaa taaatcaaga tgaaataaat acatgtcttt
     2041 attttacgaa acatcaatac taaaactgta gcaataatga aacaccccac ttgaaatata
     2101 tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat ttaaagtaat caaggttaac
     2161 ggattgattt ttgacactct ttcacctcca aaaaataaga tcgcactgtt atcttaagct
     2221 gataaaaatg caccgttatt cagataacag tgcgatctta tcaaaaagca taactgagaa
     2281 caattctcaa tgcaagcttt ttttaattaa ctcagtaatt caatccaccc aggaacagaa
     2341 aattgataca atcttctggt taatagcata gtcttttctt ttgaatagct atgcttcaat
     2401 atatcataga atgtagcgta gaatgagtaa atgtagaagt ggatttcttc ttcattaaag
     2461 actaaatttt gattgatcat tccgatagtc tttaacttat ctagacaaca gactgtcttt
     2521 ttcgtcacaa atgatgtaat tttttcaaca atatcttgat atttggttcc agctgattgg
     2581 aataaaatca aatcgaatat acgtttattc tcaaaaatat aattaataaa tggatctaat
     2641 tcattgaagt tctctctcca acttttctct atatctatat tttctaagtt taagaggtat
     2701 ttggtaaact cttcattata catattaata agcgcattaa tatgcggctg aataatttct
     2761 tcaaataacg cttccttaga agtaaaatgt ttataaaaag cacccgtagt tacgcctgcg
     2821 gtcaggcaca gttgccttat cttagtttgc tgaaatcctt ctttttcaaa atggcttagt
     2881 ccactttcta ttaacttttg gtgagttata atataatctc tggtttctat gggtacttcc
     2941 tcctaataag atgaattaac tttattatct tacacaggac ttgaagtttc aatcgttaac
     3001 gtttattact gaactcattc cttagagcta aactaatgag aaagagcaga ataactgata
     3061 caaaaagcaa tcctacaaga attaatagac tcatctaaat cattcacctt ttctaaaata
     3121 atccgagaaa gatttaaaaa aattatatcc agctattagg ataagtccta tcataatcac
     3181 tccaatagcc attttcttat cctccctaaa aataaagtct tttacttaaa accttttttg
     3241 taatcgtcaa taaatttttt ccctttatca gttaattgaa atcctatatc taaatagtcc
     3301 tcatatctaa gtttagccag catctcactt acagatgctg gactaacact tagaactttt
     3361 gctaaatcat tattttttaa tttctttttt ctttttgata attcctgaat tgctagtaaa
     3421 taaattagta tagtagtcgt tttaatttgt accataccta ccctccaaat tatttcgaaa
     3481 ggccttcagc aattttatcc aggttccatt tcatcatcgc atagtaacta tcaccatctt
     3541 gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata gataggaata ttggtatctt
     3601 ttgatactgt tttcattggt ctatcatcca cactgctttc tacgaataag gagggaactt
     3661 ttgttgtgcg taatttttca actaagtgtt ttatttgatc tggtgttcct tcttcttcag
     3721 tgttgatttc ccaaatgtaa gcagaaggca cattatacgc tttcgagaaa tatttaaagc
     3781 atccttcact tgtaacaatc atttttttat cattcggaat tgaagcaaat ttagatttag
     3841 cttctttgtc tagagaatcc aatttttcaa tatacttatc tagattttct ttatagaatt
     3901 ttttattatc aggatctttt tccgctaact gtttttcaat atttttagcg taaataatac
     3961 cgttttctaa atttaaccaa gcatgaggat cttccttccc tttctcactc tgaccctcta
     4021 agtaaataac atctatgcca tcacttgctg caaaatagtc tttgttttcc tctttgttcg
     4081 catttttaac taattttgta aaccaagcat ttcctccagt ttccaagtta acaccgttat
     4141 aaaaaatcaa atctgctttt gaagtttttt gaacatcttc aggcaaaggt tcatattcgt
     4201 ggggatcttt cccaataggt acgatactgt gtaaatcaat tttatctttt gctatatttt
     4261 cggtaatatc tgctaaaatc gaattagtaa ctactacttt taatttttcg ttcgaagcca
     4321 ctttatcttt gtctttacta tttattgaac atgccccgaa tataaaaaga gcaatcaaaa
     4381 tccccagact tcccagtact gctttccatt ttctcatcat aacgatctct ccttctttct
     4441 taaaaatttt tgttttggtg agattagaaa actaattaca aataagaatg ccgctgttaa
     4501 aacaatactt gatcctgctg ctatgttaaa actataacca ataaacaatc caagcaatga
     4561 tgaacttgct ccgactgttg cagaaataaa tatcatagtt ttcaagctat ttgcatataa
     4621 atatgctgta gctgctggtg taattaacat tgcaacaatt aatatagttc ccacactttg
     4681 catagctgtt actgctacta aagttaatag aaccatcaat agataatgat aaaaactgac
     4741 attcatccct attgcttttg ccattaatgg atcaaaagaa gtgattaata attctttaaa
     4801 aaatagaact actataacca acactaaaac cgaaacgcca attgtaatcc acttatcaat
     4861 atcttgtaca gctaaaatat ttccaaataa aatatgaaat aaatccgttg agctatttgc
     4921 aactcctata aggataactc ctagtgctaa aaaagaactg aaagtaatac caattgctgt
     4981 atctcctttt attacactat tgctttttat aaaagtaata atgatagaag ctaatagacc
     5041 aaatacaatc gcaccaataa aaaaattaat acctaaaata taagaaattg ctactcctgg
     5101 caaaacagca tgagaaatag catcacccat aagagacatt cctcttaaaa taataaaaca
     5161 acctactgtt ccagaaacaa tccctataac tacagatgta attagagcat tctgtaaaaa
     5221 gtgaaagtca tacagaccat taataaaatt ttgaagcata ttattccccc tctccaataa
     5281 atatagtatc tccataggct ttttttaaat tttcttcatt aaatacagat tctacacttc
     5341 cgtgagcaat tagatttcga ttcaaaagaa taatattatc aaaatacttc tttaccttgc
     5401 ttaaatcatg atgcacaatt agcactgttt ttccctgatg tttcaatttt tttaacgtat
     5461 ccataattat tcgttcacta actaagtcta tccctacaaa tggttcatct aaaaatatga
     5521 aatccgcatt ttgagcaaga catcttgcta aaagtacacg ttgaaattgt cctccagata
     5581 attctcctat ttgatgatta gaatattttc ccatatcgac cttttccaaa gcctttgaaa
     5641 ctctttgcca ttctgcattt ttaattcttt gaaaaacttt cattccggca tatgttccca
     5701 tagatacaca ttcttttact tttattggaa aagtaaaatc aatatcagct ttttgctcta
     5761 catatgcaat cttcaccagt tttttattga tatcttcatt attaatggag acctctccct
     5821 gatgagggat aatatttaac attgctttaa gcaatgtgga ttttccagca ccatttggac
     5881 caataatacc tgttaaagca ccttcttgta tttttaaaga aatctcgttc aatgctaaaa
     5941 aatcgttata acaaacatta agctttttca cttctaacat aactttctcc ttctaaatga
     6001 taatgattat cacgatcata tggtatacaa aaattatatt ttagtcaacc ctaaatttta
     6061 tagtttagat ttttttctgt tgctgaagat tagaatgaca aaactatttg aaactacgcg
     6121 tatagtaagt agctttcaaa atatataaaa aaggagcgtt ctcaaatgag aacatgctcc
     6181 taaaactact ttattggcgt atggaaaaat tatacttgaa gtggatagcg ggttgaataa
     6241 gttgataagc atcaaaacat ttataaaaat tattcaagac tatgtctgtt ccatatacaa
     6301 atatgaaaat cagtaaactc catttaactt ttttcgtagt gtgtcttttt atacatgttt
     6361 atcgttatta atagtgaata ggaaaaaaac tatttttgat cattgacaat taaatacaat
     6421 ttttgaataa cgatccctgc tattcgagct agaaactcaa gtacgccatg acctgattag
     6481 cgacaaatac ttagagattt ctttttgttc tgggaaaaca aaatgccatg acaatagcag
     6541 caaataatga tacttccgta aggctcgccg taaactgggg agaggtgaaa ttcctatgtg
     6601 cttacccaaa gcaattcaaa aatttcataa ctactatttt aggagatgat tttatagaat
     6661 ccagagtcta cctgaataaa aaacaaacct gtgcatttct aggaatcgat aataatactc
     6721 tagataagtg gattcgtata tataatttcc cagtaataag agttggtggt gtagttcgct
     6781 ttgacgtaga agatattaaa gactttatga atacttgaaa ttttcttctc gccgcatata
     6841 ctaaaattag tgaaatatac tgccttactt tttttaagga gtgaggattt tgcctacaaa
     6901 gctttcaaat gggaaatata aaactaatct gagatatcca aaaaggttta aagaaataac
     6961 aggaattgca tcagaaaagt atcaaaaaac atttccaaat aggcaattgg cgattaaagc
     7021 cgaaaatgat atgaagaaga aaatagaaaa agttttgaga gaggagaatg caaactcatt
     7081 agaattaaaa ggaaaaataa cgtttaaaaa attttacgaa tctaaatggc ttcctagata
     7141 tgaattaggt caaacaatta gatcaaacag gcctcctagt gatataacca ttagtaatac
     7201 aaaagatata tttcggttgc atattttacc tatgtttggt gaatacgcaa tgaattatct
     7261 aaatatcaat actgagataa tatcagatga attaaccaaa aaatcgaaag aatatgcaaa
     7321 tataaaaatc ataaaaggat atgttcgttc catgttcgat attgcagaaa tactaaatta
     7381 tatcgaattt aatcgaacta ctaaaattat tcaaagtatc actgcaccta aaaaaaatgc
     7441 tttagaagaa aaaagaatac aagaaggtaa gcaagcacta tcatcaaaag aacttactaa
     7501 ttggatagaa gcagtaaatg atgattttaa taatcattta cttacttttc atgattacac
     7561 actttttatg attaccctgt atcttggtga tagaaaaagt gagacttacg ctttgcaatg
     7621 gaaatatatt gattttgaaa aacaaacggt ccgtcttaaa catactttag ataaatatca
     7681 aagaaaaaag tttacaaaag gtcgaaaaga taccgtcatt caagtaccag aagttgtaat
     7741 gacattgcta agtgaatgga aatcggtaca agctgatcaa ttgttgaaat taaaaattaa
     7801 gcaaacgtta gatcagtatc tctttactta tactaaacca tctggagaag taaattgtcc
     7861 tgttcatgcc gattacctaa actatcgaat caattcaatt aaaagaaggc atcctgactt
     7921 agttcattta tctccacaca aactccgtca tacttatgca actatagcta gacaaggtgg
     7981 agcagatatg aatcaaattt caaatgcttt aacacactca gatatttcta caacaaagat
     8041 atatgtgaac acaccagata tagttgataa ggctgttttt gaagcttttc agagaggatt
     8101 gaataagtgt gattgatatg tgttcttaaa tgtgttctta aaaacaaaag aagcactttc
     8161 caaataattg aaaagtgcca gaaacgttga tatataaagg atattaacgt tttgagaatt
     8221 gtgaagcttt acgtgctttc ttaagacctg gttttttacg ttcgaccata cgtgcgtcac
     8281 gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact tctagcaatg
     8341 cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg ccgtttacgt
     8401 taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga ttgattactt
     8461 cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca gtaattttgc
     8521 cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct gtgccgatat
     8581 attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg tctaaaactt
     8641 ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat tttgtaaatt
     8701 gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg atcaaacgac
     8761 gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct gggtagttgc
     8821 tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact ttgtctgcat
     8881 tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt ttatttttac
     8941 cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca gttgcgtcta
     9001 ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta cgcacgttat
     9061 tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg gctctttgtg
     9121 gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa cgtttttctg
     9181 cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat aaaagggtcg
     9241 tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca caacccctta
     9301 ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat atccataaaa
     9361 cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg caggcttaca
     9421 acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa aaacggcaaa
     9481 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatttcac
     9541 aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg gcgctttgcc
     9601 caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt cgataaagat
     9661 atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt ggtaaagatc
     9721 tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag tacgatccca
     9781 gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg tagaataagg
     9841 tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt tcaaatcatc
     9901 catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca tgtaccaatg
     9961 tgtttggtga acaacaacag acatttggct tagatctgca actaattgat ttaatacttc
    10021 tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac ttaaaataat
    10081 agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt ttcagttcgc
    10141 ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg cagttgagca
    10201 aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg atagattttc
    10261 tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc tatcaattcc
    10321 tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat cacagctcct
    10381 aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac atcagccatc
    10441 atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc tgtgagctga
    10501 ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc ttctagaatc
    10561 gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact gccaaacgca
    10621 tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat cagaccattg
    10681 gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt ttctcctttt
    10741 tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc aaagatgcca
    10801 gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac ataacaaaac
    10861 gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc tccgctcgtt
    10921 cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt agaaagcggc
    10981 ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc tgctgctgtt
    11041 tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa ttgctctatt
    11101 cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat atcaaaatat
    11161 ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct ataaaagata
    11221 tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg gctgttttgc
    11281 agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt ctgccgataa
    11341 aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc tcggttatct
    11401 gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat caggccatgc
    11461 atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg tttaattact
    11521 tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc tgccatccct
    11581 tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg ggcttcatct
    11641 gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga aacgttcatt
    11701 ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt atcttggttt
    11761 tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat cggatcattt
    11821 ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc tagatctcat
    11881 tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct ctcgatcatc
    11941 gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta tttgttggca
    12001 gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc aagaaatttt
    12061 tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt attttacttc
    12121 atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt ttggttgcag
    12181 gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg tgtgtgcctt
    12241 cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc ttggagtggt
    12301 ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt tggaatatcc
    12361 gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc tttcattcct
    12421 tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa ggttgtgaca
    12481 aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg tgacttggag
    12541 ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga ccctcagtat
    12601 tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt agctttttcg
    12661 gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc agaaccttct
    12721 cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta atcttctgca
    12781 ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa tcgcccgctg
    12841 tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc attatagtag
    12901 atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc agtcggatcg
    12961 gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg atcatattcg
    13021 atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata agttggattt
    13081 ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg ctcttcaaat
    13141 ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt catataaaat
    13201 cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt gcttgtgaca
    13261 gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa aacgaacgta
    13321 cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga tttatgtttt
    13381 cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca gcttctgaat
    13441 cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct ttgagaaaaa
    13501 cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca tcaaaataac
    13561 ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat cccaatccga
    13621 tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc aaaacggatg
    13681 tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca gcaaaggctg
    13741 atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat cgctctagtg
    13801 actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat ggaatggaca
    13861 gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct acaagaacgc
    13921 catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa aatgaacgac
    13981 ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg cttgctgtaa
    14041 agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa tgccaacatc
    14101 tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag gaacgccctc
    14161 tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc catctagacg
    14221 tccttcgcct acgattacga ggtctgcttg ctgtacccgt ttatcgaact ccagtaaatc
    14281 caatacgtag tcgatgccag agactatttt ccctgaagca aatgctaaca gtcctgcacc
    14341 cattccacca ccagcactac tgcccgatgc ctcgattact tgaggggcaa atctcatata
    14401 aaaatcatgc atggcttggt cagccactgc taattcattt tctttcaagc ctttttgtgg
    14461 tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga ttagtaacat cagtaatgac
    14521 agtgattttg gctttaatca gctcaatagg cacgttcgtt ttatcaactg tagcaatttt
    14581 acctagattt tctcccagtg cttctacttt ttgattatct tgatcgtaaa aagcataacc
    14641 tagaccttct gccatgccga tgccgccatc gttggtacta cttccgccaa cgccgatgat
    14701 gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt tctcctactc ctcgcgtgga
    14761 taacctaagt ggctgttgtt ggtttagtgg gacatctttc aatccgacga tttctgccat
    14821 ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt tctttgtttt tgccaaatgc
    14881 attagtaacg actgctgttt gccgcttcaa ccttaaactg tctgctaatg atgaaaccgt
    14941 tccttctcct ccatcgccta taggtagcag atcatattca gcctgaacaa aaacttcttg
    15001 aaaacctgat tgaatggctt gtgctgcctc taaggctgtc agactttctt tgaaagaatc
    15061 tggcgcaatc agaatacgca ttattttccc ctccgttcca agatcagttc tttttccagc
    15121 tggtattggt ctccattgac aaaaataggt aatgtttctt tcgtctcttt ttttattact
    15181 atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa aggaaaagaa
    15241 attttttccc atgctgaagg aagtcttgga gaaatagaaa gagttccatt cgttactgta
    15301 atacctgaaa aaccaaatat aactgctagc catagtgctc ctaatgaagc agcatgaatt
    15361 ccgtcatctg atgaatgcgg atctggtcct aaatcaatca ggcacgcttc ttggaataaa
    15421 tgatacgccc attccatttc ttggatacgg caagcctcta tggcgtgaat cgctttactc
    15481 aacgatgagt cgtggattgt tttgttttca tagtaatgca aattcttaga aacgattggt
    15541 tgagaaaaat actcaggtaa taagtataac agcatgacaa gatctgcttg cttgctgact
    15601 tggagttcat ttacttcgct acgggcataa tcaagtaaaa tagactggct gccaggacgt
    15661 tctttgtaag tggtcaaatc aataactggc ttactgagat acgtgtcatc ttgcggaatc
    15721 agctgttctt tgttaggcac tggcaaatat aacttatcta aaaaattttg acattctttc
    15781 aaaaaaagcg gatcgccagc ttgatattta acagctaagg ccacattata atgcgccaga
    15841 tagttcgtat atgcattatt gtcgatatat tctgtgtatt catctggacc aatgactctg
    15901 tgtatctcta aatgatcatc gacttttaca gctctgctca gccaaaaagc tgctgtttca
    15961 tgaatgagcg tttttccctt tgtttctaaa aacgattgat cctctgttgc ttggacatac
    16021 tcttgaacgg cataagcaag gtctgcactg atatgatgtt ctgcggcagc tgaagcaact
    16081 tcttgtcttg ttccggtttt gatattcaat gcagcaaatt tgggtgtctc ttcctgacca
    16141 gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt gataatctct tgctttttgc
    16201 cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat tctttgctac ttcaggaaac
    16261 tgatataaaa agaaaggcag gataaaaatt tccgtatccc agaaaacatg ccctttataa
    16321 ccttcgccag ttagtccctt cgcccctaca ctaacacttg gatcattgcg gggagtcata
    16381 atctgtaaat ggtaagcagc gaagtccatc gctaactgat cgaaagaaga atcgccttgg
    16441 atcgttatcc gatgctgatg ccaaaaatct gtccaataag acatcgactg ctctaaaatt
    16501 tctgtataac taacgctttt ctgggcttgg gcaatacttt tcttaaacgg gtctgtttgc
    16561 tgaagagagg tagagagcac gatgatcttc tccagcgtaa aggacgtatt ttttctgatc
    16621 ttttctgaaa aggaagcggt gatccggcga ttttttgctt ggaatacacc tgctttattg
    16681 aatttagctg caatcccaat atcaatttta ctttctgttg tctggtagga tgcactgatc
    16741 aaatcgtcac caaatactcg cagttctttt tcaaccaact gctgtgtacc aaaattggtt
    16801 tgctgtccat cgatacccgt tttcacatct acttggcagt cttcggtcaa cggcttgatg
    16861 accactttca gggctgcctg atggagatta ttttgatcag caacacgaca agtaatgact
    16921 tccacttttt ttccttgagc agtttcaatg agcgcatagc gtttaagctc tcccatttct
    16981 aaatctagtg cccgctcgta tgcaagaact ttcatcgtct caagagaaaa atgttcaccg
    17041 tcgattttta tttcccatct gatgacatct gggagattca ctaactcggc tgattctgct
    17101 cctaaaggtc ggttatatac accagctgca aacattcccc ttacttgatt ccgatagtct
    17161 tcctcctggc ttcctctgat accaaaaaat ccatttccaa tggtcatctg actcgcatac
    17221 ttattgatat actctaaatc aaactgtttt tcttctactt tcataaggta ataacttctc
    17281 ctgtttctgc cgaacgatat aaattttcca ccagattctg gatgacatat ccttgccagc
    17341 catctgcaat catctgagtc tggtcaccga gcgcccgatt cacaaaatga gccatactta
    17401 gttcatgtgg gtcattaact ggcgtggtct tttgtaagga aaccagcttt ccatctacat
    17461 cttcgaatag ttcgcctgga tacagattag cacctgcctg atcgccacaa aaagccacat
    17521 tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt cagttggatg atcttgccgt
    17581 tttctaattc cagtgtgcca aaaattgcat cttctactgt gaatttgctt ggatcccatt
    17641 tcccgaattt ccctgaattt tttttcgtac caattttttg gaagctgtac gcccgaacgc
    17701 tttttacttt aggaaatcca gtaatataca tcgctgtatc aagcatgtga atgccataat
    17761 cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa cgttccccag cctggaacac
    17821 cagaccgacg aagcgcattc gcttctacgt aataaatatc cccgagaaca tcaagatgat
    17881 tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa tccataagcc aaaatctgcc
    17941 cgtttttatc ggctgtctct gccatctttc ttgcttgatc agctgtcata gctggtggtt
    18001 tctcgcacat cactgaagca ttagcttcga gggcagccaa tacatgttcg aagtgaaagc
    18061 gattgggtgt acagacactc acgatatcgg gagaaatctc attcagcatt tgtgtaactg
    18121 atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc tggctgactg
    18181 gatcaaccac agcggccaat tctaagacat ctgatctttt ttgataataa gcagcatgta
    18241 ctttttctgc tacttgtcca gcgcctatga ttgctacttt cttcatttat ttctccctcc
    18301 tttgcttatt tacttattgt tattctttct ctattctttc tctaaatcat ttaaatgacg
    18361 cgctgtttcc agacagtttt tcaagtattg caatgattcc tgataaattt tcacttcatt
    18421 ttctcctcgc aaacgacatt catataccat ataaccttca tactcatttt gaagtagcgt
    18481 ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt tggtagcgat gattatctgc
    18541 taaatggata tgacccacat actctttttg cgtagtcaat gcttttgaaa tatcatcttc
    18601 ttcaatattc atatgataaa aatcagcgat gatctgcaca tgtttcaatt gattagagtc
    18661 aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc ggttcaaagg
    18721 ctccaaataa ataaccgtac ctgtttcttc ggctacttta tctaaataac gaagtgattc
    18781 gctgactgct ttatagtctc cttctttgct tcggggtgaa accatcggcg gtaatcgata
    18841 agtgaacatt ccccaagctg ctgggataac gattcctttg cctccaacct cgcttagtgc
    18901 acgcaaaatc tcagcaattt gacgtacacc attaagacgc cgttcttcga taaagtcacc
    18961 gatccaccca tcgtagccgt tacaagctgt cactactggg atccctgttt ctttgattgc
    19021 ctgtttgact gtgtcgatat tttctattaa caacttaccg tcgatttcgt attcttcaaa
    19081 tccacagtct ttgataaatc taaatttttc taaaatgtct cttgggaaaa atgcttgatc
    19141 ttgcgtaccg aatttcattg ttctcctcct cggacttgaa cgcccatttt gatactttct
    19201 tctgggtgct ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa tggcacaact
    19261 ggatcaatga tttcttcaca atccaagtaa ccgttcatca ataattccca acatgtgtcc
    19321 tcgattcgtt tacggttcca tcttgggtaa tctgggtttg gttcactaga tgctcgagca
    19381 aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc agctggaaaa
    19441 ggtttagcaa aggcaacata agaaatgatc ccgccatagc cgatccctct taaagaagct
    19501 tgtaatgcgg cagcactgcc actcgtttca ataactgaat ctactccacg tttatctgtc
    19561 aattccttta gacgaaaacc gacatctgct ttcataggat ctagcgtgta atcggcaccg
    19621 tatttttctg ctaattctct acgctgcgct atgggatcga tcccaacgat ggtagtagca
    19681 cctgcttttt tagctaattg aatcgcaatt tgcccgatag cgcctaaacc gaagattgct
    19741 acagcatcac ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa ttgagcagga
    19801 tcgtaacata ctgcattttt ccattgatcc aaccgatcaa gtttacgtag tttataatta
    19861 tcaacaccgt ttgtaatcag cgtttcacga atgggtccat aagtgcagac taactctcct
    19921 ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat catatttcct
    19981 aattgaaatt cgccaaatac cacaccgcgt tggcttcctt cttctcttgg caagaatagg
    20041 cgccattctt ctgaaaattc ttcatcgata aatggactta atcctctaaa gtcgacaatt
    20101 tctgttcctg gttttggagt agcgaaaatt actcggatac gtacttcgtt cttttcgatc
    20161 tcacggtctt cgtaatcttt gaatccggct attcttggtt ctaatgcaac taattttttc
    20221 atcttttctt tcctctttct tttcttttta tccttttacg ccgccttcga ctaaatcacc
    20281 tttaataaat cgttcagata acgcatacat aatgatgact ggtaaagcgg tcaccaatga
    20341 agcggccatc attcgtcccc aaatgtagtc tggtgtactg aataatgtgt tcaaaccgat
    20401 tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat aatcattcca
    20461 cgaaaccata aagcaataaa cgaagacaga aatgattcca gaaatagcca atggtataat
    20521 gatccgaata atgatctgga tccggctaag accatcgatc attgctgctt cttcaatatc
    20581 agtcggaatc gtgtcaaaat aactttttag catgaatatc gcagtaggaa gtgtttgaac
    20641 gaccatcgtg ataatcagtg ctgtattggt atcatacaaa cctagaccgg aaatgatttt
    20701 aaataatgga acaacgatca aaataccgga aaacatataa acggtgtaaa agctagcatt
    20761 gattttcgag cgagctttga agcgtagttt tgacaaagcg tatgccccgc tgatcccaat
    20821 cactactgca gttacggagg ctatcaagga gatcactaaa ctatttttga aatacatgat
    20881 gaatggaaaa atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc attctttcgg
    20941 aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg tcatcagcat
    21001 gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt aaaataagat
    21061 tttcctgctc tttttatttt tcatttcaag tccaccttct ttcgtgagag caagacgacc
    21121 gctgcaataa tgatgaacaa tagaatcgat atagctgctg cttttccaag atcattgaac
    21181 gcaaaggctg tctgatacaa ataaacgcct aagatattga ctttattcgt cagtagatag
    21241 acatctgtaa acatatagaa catccagata gatcgtaaaa caatcacagt tgctagtact
    21301 ggtttgattg ccggcagtgt cacgatcttg aacttctgcc aagcatttgc accgtccatt
    21361 tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa tgaaataaac
    21421 gcatacggat agtatctcca gatagcaaac aaaacgacca taaagaagct agcaactggc
    21481 tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc tatctactaa cagatcattg
    21541 acgattccat aactattatt gaacatatat ttccaagcaa aaatcaaaga aatagatggc
    21601 gtaacataac ttaataaaat cagtgaacgc accgttttcc taaatctgaa cttgcggtta
    21661 agcaaaatag ctacggctaa tcctaaagct gtactgcctg ctacaactaa gaatgtgtaa
    21721 gcaactgtta aacctaaaga acgataaaat tcactatcac ttaaaatttc aagataattg
    21781 cttaatccga taaaaacggc gtctgagtta ggattcagcg gcatatcgaa aaaactgatc
    21841 tgaatattgg aaagcattgg aatcagcact aaaacagcta ataaaagcat gcttggtgct
    21901 aaaagtccca tggctaattt taagtcagaa tgtttccttg gacgattcat tgaaacctcc
    21961 ccctgataat ctgccaatag ctatctactt ttttcgaaca ccattggcag aacttcttgt
    22021 tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt tccgacagtt
    22081 acttggttca ctgcttttcc gatcacacct gagcttgtga tatctcccat tttcgtgaaa
    22141 ttcttttccc cgactagtcc aaagacttgt acttcatcaa aagcactagc aatctcagat
    22201 ggcagctcgc caaaagactt gatgacttca ttttctttgt aaccatccag ttcgactact
    22261 tgtttattca ctggttgagc accaccagga gacatcaaga cccacttttc catgtttttc
    22321 ggttgagata agaaagctgt gaatgcttca gcagcctctt tttgtttctc gtctagacca
    22381 gaagaaatcg tcaatccaga tactgtgcca tagacagctt gatctttttg tgttgggata
    22441 gcaaaaccga tattttctga gttccccgct tcataaacag aaggcaaaat gtaagttgaa
    22501 tagattgcca ttggtgctgt tccattcatg aaagcatctt tgatttctgt cacatcatta
    22561 gagcctggca tcgtgtattg ggataagttt tgataaaacg acaatgcttc tctcatcttt
    22621 tccgtatcga tcgttacctc gccttcatca tccaaaacat ttgctccatt tgacaaagca
    22681 aattgagaga acgcttgctc actcattgtt ccttcagcag taggcatagc aattccatat
    22741 tgtttattct ctttatctgt gaaatgttga gcaattttta aaatatcatc ccaattttct
    22801 ggttctgaaa aaccagcttc tgataatttt tctttgtcat accaaatacc ttgaacccag
    22861 ccgctaattg gtgcagcaat atagcttttt ccatcttctg aacgaacaag gtttttggct
    22921 ccgtcataat aattatcttc tccagcttcc tcgataacgt tttggactgc cgtctgatca
    22981 atcagttcat ctttatccat cactttagca aaatcctgac tgacttccat tacttctggt
    23041 aatttaccgg aacgagctaa cgtaacgatt tttgtattga acgctgattc ttctaccggc
    23101 acttgcttga ttttgatgtc tggattttct ttttcaaaat cagcaaccag ttcattgatc
    23161 acacttaatc gttcctgttc gacagaagag tgcatgaatt gaatcgttgt ctgttgtgat
    23221 tcgttagagc tttgactttc accacaagca gtaagaagaa atccagtagc tagcagacag
    23281 gtagtaacaa taattttttt catttttttc cacctcttat tttattattt tcagccacat
    23341 atattcatac ggctggaata tcaactttcg ttcttgactg caatggataa tttgtccaga
    23401 aaacaggtct aggtaatctc cttttggtaa aagataacct gctgattgat tggaaaaatt
    23461 atggataact aagactttct cttccttcgc ttcgcgaata aaactgataa aagaatctgc
    23521 tgagtccagt aagtaaatca aactattcgg atggaaggct ttttcttttt ttcgaataga
    23581 tagcatctgg gtcaattcac catatactgc tgttcttaat ggatctgtct gcaactcttt
    23641 ctcaatatct gctaacaaaa atttttgccg attgattttt cgattctctc ctgttttttc
    23701 tacgcctttg taatcatttc gacttcccag cacactttga atataaattg ccggcacgcc
    23761 tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc attccgtcgt
    23821 atctgttgga gaacttaacg catctagata cgtagcgttt atctcatatg gactggtcgt
    23881 tccatctggg tttttcttgt agtttactcg ggtaccctct ttcttcagct gttccactaa
    23941 ttcaaggatc gttttttccg gcagaatacc tcgtaccgga ttcaatccga taccgtcatg
    24001 ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg tttttaacca
    24061 ttctttcaac ttacttgtat tacccgaacg aatcgcttga agggtcaaag gtggtagcga
    24121 aaactgatag accatctgtg cttcattctc gccattccca aaataactga tattatcttt
    24181 gtgtggcaca tttgtctcag tgatcaatac cgttcctgga gctgcttcat cagcgattgc
    24241 acggaataat tggatgattt tatgcgtttc tggcaggtgt atacaagaag ttcctagttc
    24301 tttccataaa aatcccacag cgtccaagcg aataaatgaa gcgccttttt ctaagtaaaa
    24361 caaaagaata tcgatcattt tcaataaaac ttctggattt ttaaaattca agtctacttg
    24421 atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa tgaacgttgt
    24481 aagtaacggg ctggtccttg gtctagtcac gctgcttgta tctgtatcca gtggcaaatc
    24541 aataaaaaaa tctttgtacg tatcattcaa gtttaaatat tcctgaaacc aagtactttc
    24601 ggcagacata tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc tcatttcttc
    24661 tatatcttcc catgtgccca aacggtcgtt gattgcctga taatttacaa cggagaaacc
    24721 atcatcagat gtatatggga aaaatggcaa gaaatgtaca actctaaatg tatctgccaa
    24781 atacttttga taaaatttct taaatgtacg caaagttggt tgctgctctt caaaaaattg
    24841 atccccataa gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc ctgcctggcc
    24901 tttcttctgc tgataaacac tgactttttg ttcaatcata gaggcggttt tctcagacaa
    24961 atctgagtcg cgataaatcg cttgtaaaag ttttgtatac atcggattcc cctttctttt
    25021 ttgggagcgt tcccaacgat ttgatcatat tatggaaaac gcttactgtc aatagcattt
    25081 ttttaaataa ttttgatata cacaaaaata caaataagaa agcttatgta tcaataatta
    25141 atagcttctt aaaaaaatat ggtctctcat gatcatgcaa gctattccta aattttcaaa
    25201 atcatagaca aaaaagaatg tctattgtac tatggaatta ttctaaaacg ctcccaaaaa
    25261 ggaggtgcat ataaaattga aacctactat ttatgacatt gcgaaaaaag ctaatgtctc
    25321 aaaatcaact gtctccagag tattaaataa ccagccaaat atctctgata aagcaaaagc
    25381 aagtgtactt caagcaatca aagaattgaa ttatgaaccg agcaaaattg caagaggact
    25441 gtcaggaaat ggttttgacg cgattctagc attatctcat cgtacttctc atacgaccta
    25501 tggcaatcct tttttttcag atgttattca atccatttct gccgtttctg aacaaaataa
    25561 cttcgacttg attctacaaa cggcaaaaac accagaagaa gagctagaaa aatgcgcgaa
    25621 aaagatccaa gaaaaaatga tccgaggaat tattatttta agctctacga tggatgaaac
    25681 acttcttttt gaattggatc aatacaatat tcctattgtg gtaatcggaa aagtcggaaa
    25741 cgactacacg aacatctact cagtggacac ggataatttc caggatagtt acgatttggt
    25801 ggaaacactg atcaagaatg gacaccgtca tatcggctgt ctccatgctc cgacagaagt
    25861 ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc accacctaga
    25921 tatccgtaat gaatggatta tagacggcgg ctttcgctta gaagatagca tggcagcaat
    25981 gaatgattta atgaagctac gagaaagacc aactgcgatt ttttctaccg atgcattaaa
    26041 atcattgagt atttacaaag tggcagaaca atctggaaag cagatacctg atgatctttc
    26101 gatcgcagga ttcagtgatc ccttattgtc ctccctattt tcgccacagt tgactcggat
    26161 agacattccg acaaatcagt taggaatcaa agccacagaa cttttattcc aactaatcca
    26221 ccaccaatac cctgaagaaa aaagaatcat tctttcaacc gagattactc tcacagattc
    26281 gatccaacaa caagaaatgt agaacagaat tataaaagaa cagaaacact gaggataagt
    26341 cagttactct gttctttttt caatcactca tccctcgtta ccccctcccg gtattttagc
    26401 gaatttttct tattttccat ctgttttaga tagctgtgga taactataat gtttgtgtta
    26461 aactattttt tccacaggga agaaaaaact atttttaacc acgttgtgta taagttttta
    26521 tagttcgttt ttttaaacac atttaccctt atttcaatcc atgttatttg gggatactta
    26581 tctctaaaac ataaatagaa taacagacat gtctactggt tttgttttat tcgaggaatt
    26641 attcaataat agctagcaaa atattaaaaa taaccgctct atcttcccat caatttattt
    26701 tgcattcaaa aaatgatcta tctttacttt tgttgattga cttctcttgg aagatgatcg
    26761 ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca cttttcttct
    26821 agttagacaa attattttag acaaaccaaa aaagaggttg tgacaata
//
LOCUS       DAZKOW010000029        26611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000029 DAZKOW010000000
VERSION     DAZKOW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.029"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACG912_001195"
     CDS             complement(99..1229)
                     /locus_tag="ACG912_001195"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFQ0512528.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACG912_001196"
     CDS             complement(1878..3080)
                     /locus_tag="ACG912_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0512529.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACG912_001197"
     CDS             complement(3140..3343)
                     /locus_tag="ACG912_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0512530.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACG912_001198"
     CDS             complement(3485..4552)
                     /locus_tag="ACG912_001198"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0512531.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACG912_001199"
     CDS             complement(4610..4717)
                     /locus_tag="ACG912_001199"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512532.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACG912_001200"
     CDS             4880..5692
                     /locus_tag="ACG912_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512533.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACG912_001201"
     CDS             complement(6066..6257)
                     /locus_tag="ACG912_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512534.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
     gene            complement(6502..6732)
                     /locus_tag="ACG912_001202"
     CDS             complement(6502..6732)
                     /locus_tag="ACG912_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512535.1"
                     /translation="MNFFGDRFFQPYKINRNSIIESCDYKKIARKKKKVSIHFKYFIP
                     ISIAKKVEILYLPLTLASLANVVSILIDNKLR"
     gene            complement(6716..6913)
                     /locus_tag="ACG912_001203"
     CDS             complement(6716..6913)
                     /locus_tag="ACG912_001203"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0512536.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="ACG912_001204"
     CDS             complement(7059..7937)
                     /locus_tag="ACG912_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0512537.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="ACG912_001205"
     CDS             complement(7976..9094)
                     /locus_tag="ACG912_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFQ0512538.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="ACG912_001206"
     CDS             complement(9091..11190)
                     /locus_tag="ACG912_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFQ0512539.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="ACG912_001207"
     CDS             complement(11241..12062)
                     /locus_tag="ACG912_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0512540.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="ACG912_001208"
     CDS             complement(12059..12868)
                     /locus_tag="ACG912_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFQ0512541.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="ACG912_001209"
     CDS             complement(12889..13383)
                     /locus_tag="ACG912_001209"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0512542.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13895)
                     /locus_tag="ACG912_001210"
     CDS             complement(13395..13895)
                     /locus_tag="ACG912_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0512543.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(13960..16656)
                     /locus_tag="ACG912_001211"
     CDS             complement(13960..16656)
                     /locus_tag="ACG912_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0512544.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="ACG912_001212"
     CDS             complement(17084..18157)
                     /locus_tag="ACG912_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0512545.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="ACG912_001213"
     CDS             complement(18154..18981)
                     /locus_tag="ACG912_001213"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0512546.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="ACG912_001214"
     CDS             complement(18982..19788)
                     /locus_tag="ACG912_001214"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0512547.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="ACG912_001215"
     CDS             complement(19800..20885)
                     /locus_tag="ACG912_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512548.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="ACG912_001216"
     CDS             complement(20902..21444)
                     /locus_tag="ACG912_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFQ0512549.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="ACG912_001217"
     CDS             21702..22691
                     /locus_tag="ACG912_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFQ0512550.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIITFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="ACG912_001218"
     CDS             22688..23425
                     /locus_tag="ACG912_001218"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFQ0512551.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="ACG912_001219"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="ACG912_001219"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFQ0512552.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="ACG912_001220"
     CDS             23989..24621
                     /locus_tag="ACG912_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0512553.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="ACG912_001221"
     CDS             24706..25353
                     /locus_tag="ACG912_001221"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFQ0512554.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="ACG912_001222"
     CDS             25358..26341
                     /locus_tag="ACG912_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFQ0512555.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8143 a         5617 c         4512 g         8339 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttaccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccgaa taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
    26581 aaccaagtta aaaacataag aagctgtttt t
//
LOCUS       DAZKOW010000030        25534 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000030 DAZKOW010000000
VERSION     DAZKOW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.030"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(104..1336)
                     /locus_tag="ACG912_001223"
     CDS             complement(104..1336)
                     /locus_tag="ACG912_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFQ0512556.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="ACG912_001224"
     CDS             1591..2160
                     /locus_tag="ACG912_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0512557.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="ACG912_001225"
     CDS             complement(2338..2778)
                     /locus_tag="ACG912_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFQ0512558.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="ACG912_001226"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="ACG912_001226"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFQ0512559.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="ACG912_001227"
     CDS             3732..4655
                     /locus_tag="ACG912_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFQ0512560.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="ACG912_001228"
     CDS             4731..5870
                     /locus_tag="ACG912_001228"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFQ0512561.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="ACG912_001229"
     CDS             5863..6663
                     /locus_tag="ACG912_001229"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0512562.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="ACG912_001230"
     CDS             6663..7490
                     /locus_tag="ACG912_001230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0512563.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7495..8202
                     /locus_tag="ACG912_001231"
     CDS             7495..8202
                     /locus_tag="ACG912_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0512564.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG912_001232"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="ACG912_001232"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFQ0512565.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG912_001233"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="ACG912_001233"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFQ0512566.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG912_001234"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="ACG912_001234"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFQ0512567.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG912_001235"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="ACG912_001235"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFQ0512568.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="ACG912_001236"
     CDS             11787..13820
                     /locus_tag="ACG912_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512569.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15144
                     /locus_tag="ACG912_001237"
     CDS             13864..15144
                     /locus_tag="ACG912_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFQ0512570.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15354..16160
                     /locus_tag="ACG912_001238"
     CDS             15354..16160
                     /locus_tag="ACG912_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0512571.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16172..17392
                     /locus_tag="ACG912_001239"
     CDS             16172..17392
                     /locus_tag="ACG912_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512572.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17382..18968
                     /locus_tag="ACG912_001240"
     CDS             17382..18968
                     /locus_tag="ACG912_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0512573.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19007..21145
                     /locus_tag="ACG912_001241"
     CDS             19007..21145
                     /locus_tag="ACG912_001241"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0512574.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21513..22496)
                     /locus_tag="ACG912_001242"
     CDS             complement(21513..22496)
                     /locus_tag="ACG912_001242"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0512575.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22874..24265
                     /locus_tag="ACG912_001243"
     CDS             22874..24265
                     /locus_tag="ACG912_001243"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0512576.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24281..25321
                     /locus_tag="ACG912_001244"
     CDS             24281..25321
                     /locus_tag="ACG912_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0512577.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25340..>25534
                     /locus_tag="ACG912_001245"
     CDS             25340..>25534
                     /locus_tag="ACG912_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0512578.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNP"
BASE COUNT         8098 a         4318 c         5214 g         7904 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt atatcgtgta
    14281 tttacagaga tccaagtacc agaaggacaa agcatctggg ctccaggcaa ttcgctttct
    14341 gatatttgga tcaatacgaa tataatcgga agttctttga tgactcttgc tttattgatc
    14401 agtggatttg ccagttcttt tgaacgatgg tatgtacgaa tcatcggtgc agtagcagtt
    14461 gttgcagctt ttctcgggat ttgggtctgc caatctagag gtgcattagt cgcgttgatt
    14521 gtttttgcga tattggatct tttacctaaa tcgttgatgc gtgtcattcg tgcgccattt
    14581 attgcttaca cggttacgat tcttttagct ttgccgattt cttatctggc ggctgtctct
    14641 gaaaaagtca atttgtttac cggtcgagaa gatatttggc ataaattcta tcagacgcta
    14701 ggagaaaaat ctgaacaaat tttactagga atgaaaacat ttatcttcca acgaggaaac
    14761 caatttttag gaaaccacaa tagttataat tctattttga atatctatgg attgatagga
    14821 tttgggattg ctgctttgct gcttatcctt tttattggtc gcttaacgtt gaaagcagat
    14881 ttatccaacg ggcagatgac ttttatctgg gccttttttg cggtgatgat gcagtcattt
    14941 atggaggata cattgacttc atacccatgg gttccgatta tttatatcct gttggctatg
    15001 gccgttcatc gatatgatga accagaagtt gcggaggaat tctatgttat ggaagaggca
    15061 tatgacgata tgacttatga tgatacatct tatgaggaac aaccagatag cgctcattct
    15121 tccaggatcc aacgctatca ttgataggca caaaatacga gagaggctgt gacaaccgtc
    15181 gcagcctttt ttggtttatt gcaattgtct agcatcttct gtaaaataga aacgttagag
    15241 aaaatcttgt atcatagaaa attaagaaag aaataagaac gcagctcggt accaaaaata
    15301 tggtaaaatg gcacttgact gtaattcact gaaaatcgct tggaggaaca caaatgttta
    15361 aagatttgta tttatttatg aaggacattt atcagaacag aaaattattg ctacagtttt
    15421 cgatcaatga cttcaaatca agatatgcag gatcgttttt aggaatctta tgggcgttta
    15481 tcaatccgct atttactgta ttgatttatt ggctggtatt tggtgttgga ctgaaagcac
    15541 aaatgatcga tgcaaaatac ccatttatcg tatatttagt taccgggatg attccctggt
    15601 tcttcttctc agatgcattt gcatcaacta cggttgtttt tcgggaatat acttatttag
    15661 taaagaaagt cgtattcaat attcgtatct tgcccacgac gaaaatccta tccaatctgt
    15721 acacccactt attcttcatt ctgattggca tagctgtcgc gcttataaat ggtatatatc
    15781 cttcagcaat gtctttacag ctgatttatt atttattttg catgtgtgct ttcttgacag
    15841 gattgacttg gctgacggca tcgatccaac catttttacc tgatatcatg caattaatta
    15901 cgatcgtgct gcagttgatc atgtggacat taccgatatt atggagccct aatcagtttg
    15961 attcaggtct tgtaagcata ttgaaattaa atccgctgta ctatgttgtt caaggttatc
    16021 gtgagtcatt tttaagtgaa gcttggttct gggagcatgg aacatatact ttgtatttct
    16081 gggttttcac gttagtaatg ttcgtaatcg gctctgcagt gttccgtaga ttgaaaccac
    16141 atttttcaga tgtgctgtaa ggagaatagc tatgacagaa tatgcaatta gactaaaaga
    16201 tatcacaaaa tcttataata tgtacgcaaa accttcggat cgatttcgtg aagccctcaa
    16261 tccttttaag aaatcctatc atgatgtatt ttatgcgttg aaaaacgtga atgtagaaat
    16321 aaaaaaaggt gagatgatcg gtttcgtggg agaaaacgga tcgggaaaat ctacaatgtt
    16381 gaaaatcatt acgggagtac tgacaccaac atccggcacg atggaaatcg aaggaaaaat
    16441 ctcagctttg ctagaactag gatcaggatt caatcctgag tattctggct atgaaaatat
    16501 ctatttaaat ggaatggttc taggattttc aagagaagaa gtcgatgaaa tggtcgatga
    16561 tatcattgaa tttgctgaca tcggagacca tatttatcaa cctgtaaaaa cttattcaag
    16621 cgggatgttc gttcgtctag cttttgctgt agcaatcaat gtgaatcccg atattttgat
    16681 tgtggatgaa gcattggcag taggcgatct tgaatttcaa ctaaaatgta tggaaaaatt
    16741 tacagaaatc aagaactcgg gaaagacgat tttattcgtt tctcatgata tcagttcgat
    16801 tcgtcgtttt tgtgatcgtt cctattggtt gaaaaacgga gaagtcgtcg aatatggcga
    16861 tacgatggaa gtaacgacga attatgaaaa ctttttaaag aaaaaatcag ttaaaacagt
    16921 ggatcggaat aaaaaccaag tagaacggtt cgccgctcca gatatcgtcg acgtgacgaa
    16981 agcggaattg ttggataaag acaaaaatcc ggtggatatg ctggaacaag gagaaaaact
    17041 atacgttaaa gtaacttatg aagtaaaaga tgatacaatc aaaaatccgg ttttaggaat
    17101 tgcgctacga acagtcgaca atttttatgt atgcggactt aatactctac tagatggtat
    17161 caagattcct tggaaaaaag gagagaatat cttttatctg gagtatgaaa agttaggttt
    17221 gttagctggt gaatattatt ttgacgttgc ggtctttgaa gaaaatgcaa cggttccttt
    17281 ggtatataag acaaagtaca tgaacttgtt tgtcagcggt tcttatatcg gagaaggcat
    17341 cgtggtctta gaccataaat gggaagaagg gactcatagc aatgaaatat gattttgaaa
    17401 tggacctaga tgaacaaagc agtgtgggaa aaattgccgc acagatcaaa ccaggaagta
    17461 aagttttaga gtttggtccc ggaaacggcc gattaacgaa acatttaatc ggagcaaaac
    17521 aatgtgaagt gagcattgtt gagttagata aggaattgtt cgattttgtc agcgaatttg
    17581 ctcaagatgg attttatggt gatatcgaaa gttttgagtg ggcaaactat tatgctggac
    17641 agacatttga ttacgtcttg tttgcggatg ttttagaaca tttggtagat cctggaaaaa
    17701 cattgaagaa agtcagagaa tttttgaatg aagaaggaga gatcctgatt acattcccta
    17761 acttggcgca taattctgtc atgatcgatt tgttcaataa ccaattgccg tgggcttctt
    17821 atggtctcct agatgaaacc cataattctt tttatactca tgatggcttc caaaaagttt
    17881 ttgaaaaagc gggattattc attaatattg aagattattt gtacttagct gtaggtgata
    17941 cagaattaaa atcaacttat gaagaattgc cggaagctgt acgctatgat ttcaaaatgc
    18001 gaccatttgg tgaagtctac caatactttt tctcattgat gaaacatcca gtagcacaaa
    18061 gttccattgc agagccacaa aattctaatt atgtaaaagt gttagaagta acccagcaaa
    18121 cgaaacaaga tgaaacaatc caacaaatac cattcaataa tttcacaggg gaaaatgaga
    18181 cactaagttt ccccgtatca gaaacaacag aaagaatggt gtttcgcttc gcgcaacaac
    18241 ctagctttat cgaattcagc gcagaagctg ccggagaaaa actaacattt atcgattcga
    18301 atgctgtagt caaaacagta aatgattgtt atttgtttga tgggaaagaa ttaccagagt
    18361 tcgttctaac ggatatttct gggaaagaag tgactatcca ttgtcactat cgctttatcg
    18421 gggaattgac accaacaatg aaagaattgt tggaaacgat tcgtcccatg gctgaagtca
    18481 caaagcagct ttctgagaaa aatgacgaat tagaaagaga aaatcatcat cttcttgagg
    18541 aaaatacaag actagaccat acgttaaaaa caaccacaaa tcgctattgc acattgttgg
    18601 aaagtgatga atggacaata aaacatagac taggacgacg taagaaagaa acttcgaaaa
    18661 aaatccaaga aaaagaactt tctatatgta ttgacgagaa aatctgggat gcagaaacaa
    18721 aaatattaaa aatcatcgga tggggaatcg ctaattcgga tcgccagccc ttgtcctata
    18781 agttatcagc tgaccaatcg ccatttttcg aagcaattcc tgtttcacga gaagaagtca
    18841 atcaagcaga acagctcgca aaaggaacag aagcgggatt tgagttacga atcctttgcg
    18901 agcaagaacg ttcattctta gtggaagcag tagctcaaaa tggccaatca tggatcattg
    18961 aaatgtaaat aatgattaac ttataaagat aaggagtaaa cgaatcgtgc aaaaaaaaga
    19021 aattgccgta tttatcgatc aaatcactcg tgaacgggcc actggcgatt tgcttattgt
    19081 gggctgggca attgatgaag tgaccaaaga aatccctacg ataaaggtag aaaaagaaaa
    19141 tgtgattgct gaagcaacac atgtcgtaag attggatatc aatcatttat ataatcttga
    19201 tgttaagacc caaagcggtt ttaaggtacg tttatcaggg aaaatgagag gaaaagccat
    19261 cttagacttc caaacagcga gacatcaaaa tggcatagct gtgaaattaa acggtaaata
    19321 cccttatgat gacggcattg aatcttcatg ggaaagaaaa aaaagattac tgaaaaaagg
    19381 gataaattat gcaaggactc atggcgtaaa aaaagcgatc cgtcgggtca aattggaatt
    19441 gaagccagga tcgatcgact atgcagagtg gatcagtcgt catgagaaac cagatttgaa
    19501 agagcaaagg aaagaagcac aaggatttgc gtaccgtcca ttgatttcca tacttatgcc
    19561 tgtctataac gttgaaatca aatggctcga aaaatgcatt gattcggtac tggatcaaac
    19621 gtatgatcat tgggaactat gtatcagtga tgatgcttct actgatccag ctatccgaaa
    19681 gtgtctggaa tcctatcaag caaaagatga tagaatcaaa gtcgttttcc gaaaagaaaa
    19741 tggacacatc agtctggcaa ctaattctgc attggaaata gcagaaggag aatttatagc
    19801 actcttagat aatgatgatg aattaccgcc atttgcatta tatgaagtgg caaaagtgct
    19861 aaacgtacat ccagaactgg atgtgatcta cagtgacgaa gataaaatcg atgcagatgg
    19921 caatcgcttt gatccgcatt tcaaagcaga ctggtcgcct gatacattga tgggaaataa
    19981 ttatatctct cacttaggcg tatatcgaac aagcattgtt aaagaacttg gcggtttccg
    20041 taaaggctat gaaggttctc aagattatga tttggtcctt cgtgtgacag aacaaatccc
    20101 tgcagatcat atttaccaca ttgaccgagt cttgtatcat tggcggacga tcccaggatc
    20161 aactgcaagt aatggcgaag ccaaaagtta tatctatgat tccggagtga aagcattgac
    20221 agatgccctg tctagaagaa acatcaaagg aagcgtacgt cctggcagaa tttctggatt
    20281 ttatgagatt gcttatgacg tcttgcaaga ggatttggtc agtgtgatca ttccaacgaa
    20341 gaatggctat gaagatctaa aaacttgtgt ggattcgatc atcgaaaaaa cgtcttattc
    20401 aaattatgaa atcattattg cggataacgg aagtacagat ccaaaaatgc aagaattatt
    20461 tgcagaatac aagcatcagc tgaaagaccg ctttatcgta gaattgattg acattccatt
    20521 caattattct cgaattaaca atttagcagc agaaaaagca aacggcaaat actttttatt
    20581 cttgaataac gatacagaag tgattgagcc agattggatg acggtgatgg tttcttatgc
    20641 acagtttgat cgaatcggct gtgtaggagc aaaactgttt tatccagatg atacaacgca
    20701 gcatgcagga gtattattag gaatcggtgg tgttgcaggg catgcattga ataactatga
    20761 tcgaacacat tgtggttatt tcggccgatt ggtcattgac gtcaattact tagcagtcac
    20821 tgcagcttgt atgatggtca aggcagctga cttcaatgct gttaacgggt ttgatgaaac
    20881 attagaagta gcattcaacg atgttgactt gtgcttgaaa gtttatgaac tgggtcgtta
    20941 caatgtctat gcacatcaag cagaattgta ccatttcgaa tctaaatcaa gaggttatga
    21001 agatactcca gaaaaacaaa gacgttttgc tggtgaaatc aaaaaaatgc aagataaatg
    21061 gccagcatat attgcgcatg atccatttta caatgataat ttaacaaaag aaggaatcgg
    21121 cgacttttcg ttgagaccag attaaactat aataaaaagc tgttatgttg tgattcgctc
    21181 gcacataacg gctttttata gttttttgtc aaaaaaaggc agaaggtgcc gactcaaaaa
    21241 aataagtcga ggttgggaca gaagtgttta actccgagaa ataagaagaa attcacgaaa
    21301 attgcttttc aaatttttgt gaatttcggc ttatttaaga agaagttgct tctgctccca
    21361 ccgtttattc gtttttaaag aatgagacaa aagtgtttct tacttttgtc ccacgctctt
    21421 attcatgctc agagcgccaa tagatacaaa atcatagcca aagtttcacg aaccctagct
    21481 gtcaaagaat cgatatttta ttttaatata ggctatttta aaataaatgg tttaaggtat
    21541 tcctctacaa aagccactct atcaattatc atgggtttaa aataagaagt gatggagata
    21601 accagattgt tcttagcgtc aatataaaga acatttccac tattaccaat tgctgaatat
    21661 atttcgttct cagaatctaa tacccaccat aaatagccat atctcatatt ttgtaagtct
    21721 tctaaagcac tggttctagg tttgaacatc tcgtttatcc acatgggtgg tacgatttgt
    21781 tttcctttat acatcccttt atttaggcat aatacaccaa tttttgccag atcacttgcc
    21841 gataaacaca aaccatatcc tgccgtcccg atatttagtg gatcacaaaa ccagacattg
    21901 gttttaggag acttatccat tgtaaactgc ttatgttctt cagctgtttc agcataataa
    21961 ttgacatggt ttggtatccc tataggatca aatagatact tattggcaaa atcaacagtt
    22021 gttagcttac ttgtctgata caacaagcca gttaatatgt gtaaacagac agtttgatag
    22081 ttgaattcgc cggttatacc ttttcttcct cccaataaat ctagtgatgt ttttgtccaa
    22141 tcttcacttg aacagacttt tgtccatgga tcgcctttac atttgtaggg tgctttcatt
    22201 gttagcaagt gcttgattgt aatagcgtaa atagttttct ccccgcgttt cactttatac
    22261 tctggaaaat aatctaatac tttatcatcg atacttttga ttaatccttg ctctaaagca
    22321 atgccaatca gcaaagatac aatacttttc gttgctgaca tgatatgaca agtatcgtct
    22381 tttttataac cattccattc atctgtataa acttctaccc catccttata tgcgacaatt
    22441 tgacatatat ttggttgtcg aaaaccaata aaatcatgca actcttgttt attcataaga
    22501 tattacctca ctttcttcta taagcataat gtcattaaaa atcgatttca attttttcga
    22561 ttcttcacgt aatacaggta ttcttatgga ctagacagcg ctttgggaag atggattctc
    22621 tgaaaaatat aaaggttcac tttatgagtg gataaaaaaa gcagcaaata cttcgacttc
    22681 tttaatttac caacattagt gaaaatcgat tcatctggag atttgtaatt tagagtcctt
    22741 gtcttgatta gaggtgtata aagcttttta ttagacattt ttaaattcca ttacaatttg
    22801 cttataaact aggtttttaa aggtgactat ggtaagataa tagagatatt attttgtgat
    22861 taggagtgta tatatgaata aaaatggaga atggagtgct gcacgacgga tattcattat
    22921 attaatggat gttattgtat acaatgcagc aatatatagt agtttcttac tgaagtttcg
    22981 tggagagatc ccattacgaa atttagaaac tttccaacac tcagcaatta ttatttcagt
    23041 gatttttatt gcgttgaaca ttttgttggg tgcatatgtt ttttataatc gaatgatcag
    23101 tgacatcgtg tttgtcacag tcatcggtca ggtattgatg acactaggga tcatggtagt
    23161 gacttttgcg ggccgctggt ttgcttttcc acgtcttgtt atcttacttt catttgttct
    23221 cggtacgctt ttcttaagta tctatcgtat tctcgtatat aaactttatt tacgcgtaag
    23281 tcgagataaa aaggtcgtca ttattgggtt agaaaaggat gtcgcaccag caatccaaaa
    23341 ttttggttca aagaaaaaca acaaacataa agtcgaagca gtcattcttg ataactatta
    23401 tgcaaacatc aaaaatatga ttaaagacat cgatattgtc tatcttgctt ctcatatcga
    23461 agaaactgaa aaaatcaaaa tctatcaatt agttactaaa aatgaaaaaa aattgttttt
    23521 gaatacaaca tttgaaaact tgacattgat caatccaaat attatgaatt tcgaagatga
    23581 aagtattatt gaagcatctg gatttagaat ccaacctgaa tatgaactat tcaaacgttt
    23641 gtttgatatc atcgtttcac ttgttttatt agttattgct tcaccattta tgttattcac
    23701 agcaatttta gtcaaagtga catcaccggg accaatcatc tataaacaag tgcggatcac
    23761 aaaaaatcaa aaggaatttt caatctataa attccgctcg atgaccgcca cagccgaagc
    23821 aaaatctgga ccagttttag ctaagagcaa tgatgcacga gtaacgcctg taggtaaatt
    23881 tattcgagct gtccgctttg atgaattacc acaaatcttc aatgtgctga aaggcgatat
    23941 gtcgatcgta ggaccacgtc ctgaacgtcc tttcttcgtc gatcagttca atgcagaaaa
    24001 cccttattac tacttacggc ataatgttcg tgctggtatc actgggtatg cacaagtcta
    24061 tggaaaatat gtctctgatt acaacagtaa attacgtttt gatttacttt atatcaaaaa
    24121 atattcattg atgcttgatt taaagatctt gctgcaaacc atcaagatcc tatttgataa
    24181 aatgtcctct caaggacttg aagaagacga aaatgtgatc tcattggcag aaattgcttt
    24241 tccgaatgaa aaaatctttc gctagttggg aggcgtaaaa atgaacaatc cgaaattcag
    24301 cataatcgta ccagtataca atgcagaagc aactattggt cgaacgattg atattctaaa
    24361 agaattggat tatgaagagt acgaagtcgt tttagtaaat gatggatcaa cagatcaaac
    24421 acaggaaatc attcaagaac tgattgaaaa tgatgaacgt tttgaattga ttactacgac
    24481 caatcaaggg cccggattag caagaaacga aggaataaga cagtcaaaag gagaatatct
    24541 cttattcttc gatgcagatg acacgccagt aaaaacgatc ttgtcagatt atgatcattt
    24601 gctagaaaag agtccaaaag cggatcttgt tgttagttcg tttgtttttc gtacaatgga
    24661 cgagggaaga atcgtttccg aaaaagaaaa cttagttaca gaatgtcact atctaactca
    24721 cgaagagttt ataaaagaca tgtatcagtt aatgaatgaa cagttgatgt atgttgtatg
    24781 gaacaaatgc tatcgtagcg atattctgaa aaataaccaa attctattta aaacttataa
    24841 tagttgtgag gatcgaatat ttaacttaca ttattatgaa tactgtcatc aagtgatcat
    24901 gaacccaaaa gtagaatata tttatgaatt tgaaggtggg aaggggatca ctaatcaata
    24961 ccgacctaac aagttcgaaa catttaaaga attctattca ttagcaaata ctgtaacaga
    25021 cgatattaat aaagacggta tggcggcgct tcttttaaaa gggacaactt cagttatttt
    25081 ttctatttat agtacctctg caagaacttc aaaagagaag aaagacgaag caaagcagat
    25141 attgagtgat ccagtgatcc gagaagccaa gcaaattgct aagacggatt cttcagcaaa
    25201 aaaaataaca aagttgcttt acaatatgcc agctccacta tttttatctg ctgtaaaagt
    25261 tggaagcttc gttgaagtga aaatgccagg gttaatggct gctttgaagc gagtatatta
    25321 gttaatggag gaaaatataa tgaaaagaat tttgcatttt cagggacgaa tgggtttagg
    25381 tggagcagag tctttcatga tgaatttata tcgtaaaatt gatcgtacaa actatcaatt
    25441 tgattttttg atttatgaag attatgcaga tgttcaagac tatcattcag agattgaaag
    25501 attaggaggc cgtatttttg ttgtacctaa tcct
//
LOCUS       DAZKOW010000031        25222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000031 DAZKOW010000000
VERSION     DAZKOW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.031"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(164..388)
                     /locus_tag="ACG912_001246"
     CDS             complement(164..388)
                     /locus_tag="ACG912_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFQ0512579.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="ACG912_001247"
     CDS             complement(586..2166)
                     /locus_tag="ACG912_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFQ0512580.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="ACG912_001248"
     CDS             complement(2567..3943)
                     /locus_tag="ACG912_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0512581.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5159)
                     /locus_tag="ACG912_001249"
     CDS             complement(4071..5159)
                     /locus_tag="ACG912_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0512582.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="ACG912_001250"
     CDS             5331..5765
                     /locus_tag="ACG912_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0512583.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="ACG912_001251"
     CDS             complement(5843..6376)
                     /locus_tag="ACG912_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFQ0512584.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG912_001252"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="ACG912_001252"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFQ0512585.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG912_001253"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="ACG912_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFQ0512586.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG912_001254"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="ACG912_001254"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0512587.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="ACG912_001255"
     CDS             complement(10101..10679)
                     /locus_tag="ACG912_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFQ0512588.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="ACG912_001256"
     CDS             11001..11471
                     /locus_tag="ACG912_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFQ0512589.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="ACG912_001257"
     CDS             complement(11579..12805)
                     /locus_tag="ACG912_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0512590.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="ACG912_001258"
     CDS             complement(13559..13759)
                     /locus_tag="ACG912_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0512591.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="ACG912_001259"
     CDS             complement(13893..14501)
                     /locus_tag="ACG912_001259"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFQ0512592.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="ACG912_001260"
     CDS             complement(14586..15056)
                     /locus_tag="ACG912_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFQ0512593.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="ACG912_001261"
     CDS             complement(15104..16018)
                     /locus_tag="ACG912_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFQ0512594.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="ACG912_001262"
     CDS             16164..16967
                     /locus_tag="ACG912_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFQ0512595.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="ACG912_001263"
     CDS             17145..18092
                     /locus_tag="ACG912_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFQ0512596.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(18160..18453)
                     /locus_tag="ACG912_001264"
     CDS             complement(18160..18453)
                     /locus_tag="ACG912_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFQ0512597.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(18481..19146)
                     /locus_tag="ACG912_001265"
     CDS             complement(18481..19146)
                     /locus_tag="ACG912_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFQ0512598.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(19286..19918)
                     /locus_tag="ACG912_001266"
     CDS             complement(19286..19918)
                     /locus_tag="ACG912_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0512599.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG912_001267"
     CDS             complement(19925..20992)
                     /gene="liaS"
                     /locus_tag="ACG912_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFQ0512600.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG912_001268"
     CDS             complement(20989..21720)
                     /gene="liaF"
                     /locus_tag="ACG912_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFQ0512601.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG912_001269"
     CDS             complement(21889..22368)
                     /gene="greA"
                     /locus_tag="ACG912_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFQ0512602.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG912_001270"
     CDS             complement(22504..23679)
                     /gene="mltG"
                     /locus_tag="ACG912_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFQ0512603.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            23883..25052
                     /locus_tag="ACG912_001271"
     CDS             23883..25052
                     /locus_tag="ACG912_001271"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0512604.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT         7576 a         5089 c         4061 g         8496 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aacaatattt tcaaaagaaa
    18121 aaagagaagg tgacaaaaat tgtgtcaccc tctctttttt tattgttttt taaagtgata
    18181 ggttggctcc tccaggttgg gatcataatc aacaagtaaa tcatatcctt taaagaacca
    18241 ttcatctgct tcctcgataa aaaaaagtgt cccatcaatt tccactttct ttacaggatg
    18301 ttctggctgt tcaactgaca ttccaatcga aaaaccatca tgaacctctg ttttgccgta
    18361 gacttttcca aaaaaacgta ttccgtaagt ttctggcaga tcaatttctg agtcaaacca
    18421 cttttgtgct tttggcgtta ccgttaattt catcccaaca acctactttc tcttttcttt
    18481 ctattgattc atcttatcgt ctagaatatc cacatcgcct gtctctccaa taaccagtag
    18541 attatcattt tcacgaacat attcatctgc tgcaggagaa gcgataaccg tttgatctac
    18601 tcttcgtatg gcgactactg ttaagtcaaa acgttgcctg aaatttaatt cagctaatgt
    18661 tttgttgaaa aatttaggat tggagactcg gatttctgct aaagaatatt tatctgacaa
    18721 ctctaggtaa tctaaaatat tctttgacac taaattatgc gcaatcctta ttcccatgtc
    18781 tcgctcggga tggacgacgc gatcagctcc gatcttttca agtactctgg catgatactc
    18841 attttgggct tttgctaaaa tatttggtac gcccatttcc tttaccatca gagtaactaa
    18901 gatactggct tgaatatctt ctccaatagc gacaattaca tgatcgaaat ttcttatgcc
    18961 taatgatcga agtgtcattt catcctgtgc atttgccaca acagcatgag tggctatatt
    19021 catatattca ttgacccgat cttcattact atcaatagcc agtacttctt gaccagcttc
    19081 gatcagtgtt cggcagatac ttccgccaaa tcgtccaaga cctacaattg caaaattttg
    19141 tttcaccgtt ttcctccttt agtatcttct tttctcagta taacaaagcc tttacaaaaa
    19201 caaaagcaag catcattatt gtaaaaaagc caagaaaaat aatagttatt ttctcagctt
    19261 ttgattatcc tctttatcat cgtttttatt tcaccaaacc atgtttgaat gcatagatag
    19321 cggcttgtgt acggtcttcc acttctagct tagccaatat attggaaaca tgtgttttta
    19381 ctgttttcag tgtaataaac aattcgtccg ctatttcctg attacttttt ccttcagaaa
    19441 tcagcattaa aatttcattt tcccgattcg ttagttcttc atgtaataca ggctcatttc
    19501 gccgactcat tttttccatc atttttgttg tcacttctgg ttctaatact cgctcgccac
    19561 gttgcgttgc ccttatagca tcagcaattt catgtgcggt agaggtcttc aacaaatacc
    19621 ctgctgcacc tgcttcaatg gcaggataaa ctttttcgtc atcaataaaa ctggttacga
    19681 taatgatttt agcttctggc caatctttta agattgcttt tgttgattcg ataccatcca
    19741 tttcttccat gactaagtcc attaaaatga cgtccggtcg aagtgtcatc gccttctcat
    19801 agccttgtcg tccattttca gcttctccaa ttacttcaat gtcttcttga atagataaat
    19861 aaaaagagac gcctaagcgc accatttcat ggtcatctac taataaaact tttatcatcc
    19921 ttatctatgc ctccttcatc aaagggacct tgatttctac acttgtacct tgacctttaa
    19981 aactaatgat ttttactgtt ccaccaatac cagctactcg ttctttgata ttgttcaatc
    20041 cataacttcc cgttttcgtt tcattcatat taaaccctgt tccgtcgtca ataatacgta
    20101 acaaaagatt attgtctatt ttgtgcaaat atacctctaa ttcattggct ttagcatgtc
    20161 ttaatgtatt tgataacaat tcttgaacga ttcggaacaa gtgatcctca atggaactag
    20221 atagttttac atcttctaca tcccatttca gcgaaatctg gattttattt tgcaattcct
    20281 tcaataactg ctcgatacct tgctttaaac ttttctcttc taaattgact ggacgtagat
    20341 gaagaagcag cgcacgcatc tcagactggg aagcgttgat gatctctgct accattttca
    20401 actgcttgcg aaacatttct ggtgtctcgc ttttttctgc ttgctccgtc aatgccgaca
    20461 tcatcatcat tgctgcaaac aattgttgcg aaactgaatc atgcagctca cgggctaatc
    20521 gatgtctttc taattctaaa atttcttctt tggtttgtcc atctacatat tggggacggc
    20581 tcgttacaat ttgtaattca ctagatactt caatcatttt ttcttttatc ttagtgattt
    20641 ccttatcaat atctttgatg tataaatcgt ccgatgcact tgggatcgga agaagcaaca
    20701 atttggattc ataattgcca gaagaaagtg cacgcagctt ttcttcgatt ttcccgtatt
    20761 ttgtcttttg aataagtgat aataatagaa agacaattaa tcctacacct aaggaaatgg
    20821 ctaacaaatg gaagataagc ggaacatata ataaacgtgc acgcagtaat tctccccacc
    20881 aatgactttg attgctggca taaaaataag tgaaaagtga aaatagaata ataaggaaag
    20941 ccgcaatccc cgaataaaca gctagcatcg ctctggatat ttttcccatc atattcgtat
    21001 cacctcgaca tctccaatca aggtattagt aataatcttc aatctacgag ggttttcatc
    21061 ataatcactg ctgtagattt tgatttgttc atttttcaaa gaaaattgct cttcttcaaa
    21121 caagacattt cctactaatg tcgcatgctc taatcggatg gctacgccca aaggtactaa
    21181 gatccgtgtt ctgcctatcc cttttcttac aatcacaata ttatcatctt ttggcaaaag
    21241 cgtattgccc aaatctatga tagtatcacc agaaatgacg gcgatgttga tatcgtccca
    21301 ttcatagacg tcgtttccaa tacgttggtt accgaataat tgctgtcttt tccgctcatt
    21361 attatgtgtt tttaattgct ctgtttggat catcatgatc tgtttctttc gccaaaaagc
    21421 atttttggtt aaatctatcc ctgaaatctc tacacctttc agaccaataa ataaaacggc
    21481 aaatacagcc atcatccata aagcaggact gttgactaag ctaaaaaaaa tgaccaaact
    21541 tcctaatatc aattgaaaat tttgaaattt agtacgagga tgtttgcgca tagctagata
    21601 tatattgaaa ataccgaata tcactaaaag caaaagtcca gtattgttta cgatttgcca
    21661 aacagcaaat aacagcagta gtgcttcaac taccacaaaa aaacgccaag aactattcat
    21721 cgctctttct ccctcctgac acattctcat ttcatcatac ctattttttt aactgttggc
    21781 tataggactt tagatgtaag atcgtttgtt gaaatctaaa atacacaaag agaaaaagat
    21841 ctagagtgta tcatcagtaa gatgaactct ctagatcttt cagtcagact acgcttgttc
    21901 tactttgacg attttaacta acatatcgcc acctggagta gcaatagtta cttcatcatt
    21961 tttacgtttg ccaatcaatg cttgcgcaat aggagaatca ttggaaattt tgccagaaaa
    22021 cggatctgct tcagcgcttc cgacaatcgt gtactcttct tcttctccat ctggtagttc
    22081 gatgaatgta actgttttac caattgatac ctcatctgca tctacaccgt cgttatcgat
    22141 gatttgagca aaacgaatca tattttcaat tgttgtgatt cttccttcaa caaatgcttg
    22201 ttcatctttc gctgattcat attcagagtt ttcggaaaga tctccgaaac cgcgggcaat
    22261 tttgattcgt tcaatgattt ctccgcgttt gactgttttt agttcttcta attctttttc
    22321 taatttttct ttaccttcta gagtcattgg ataaactttc tctaccatgt gtaacgtctc
    22381 cttttttcga taaaataccg caaaaggtaa tcttttgagg aatatagtcc aaaaaagatt
    22441 accacgcttt tcttcaaatg taaatgtttt tctaaaaatg atcaatgtct agagcctgtt
    22501 tttctaagaa ttatttacat atttttccac taattccata tgttcttcat aagttttagc
    22561 gaaataaaca tttccagtgc tcgtatctgc tacgaaataa taatagtcat tttctgctgg
    22621 ttctaatact gccttgattg cctcttcgct tggattgtca aaaggtccag gtccatagcc
    22681 ggtattggta tacaaattat atggtgaatc tacagctgta tcttcatacg tcactaattc
    22741 tttgtgttca cctaatgcgt ataaaacaga gatatctgac tgaagtggca tatttgcttt
    22801 gattcggtta aagaagactt gtgcaatgtt tttacgatca ttttctttta cgccttcttt
    22861 ttcaactaga gaagctagag ttaaaacttc ttgtacagtc aaatttttct gcttgatagc
    22921 tgaataatag ttttgcatga ccgtattcgt cttgttcacc atttcaatca ctagatcctt
    22981 aagcgaagta ctcgtgtaat aatcatatgt ggctgggaat aagtaacctt ccaaacggta
    23041 ttttgtgttc tcagctttag atgcactttc caacagttct gggaattttt ggtgcaactc
    23101 gttaaagaaa gtctcatcat tcactaaatc taaaaattct ttcttgtctt taccagttac
    23161 cttcgctacc cgttcagcaa tctgatcgat atcgtaccct tctggaatgg caattttccc
    23221 atctgcaact tttgttggct cagaagtacc gccttcttgc aactgtttgc caatctcgtc
    23281 aagagtcata ttaggcgcta actgataata gccagcttga aagccagtta aatttttaaa
    23341 ttttgtataa taattgaaaa caatcccgct tttaataata ttgtccttct ccaaaatctc
    23401 accaatctgc ttgttagaag agccactcgg gatctcgact tggactaatt ggtcatctga
    23461 tttatctaaa ggcttcaacc cgctatctac ataacgatac acggtaaatc caagaaatcc
    23521 gccaataatc aataatgtaa gagcaatcac taaaatgatc ttcctcacaa tcttatcttc
    23581 ttttttacgc agatttctcc gcttgtcgat tatatctgct gtagctgcct tactggcttc
    23641 ttttttatct ttacttgggt gttgtttgtt tcctgacaaa acaactcctc cttactatca
    23701 agttcgtaac tttattcatt atacatgata tttgaattgg cgtgaatgat tttatttaaa
    23761 tattctatca aaaatgtaag tagtaaatcc attactataa gattaaaata aatgttatca
    23821 agatacaagc tattttagct ttattctttc tgtaaaaaag aaaggagact aaataaacta
    23881 ttatgaaaaa aggaaaacat gctaaaaaaa gaaaatattc atttttaatg atcgttgtcg
    23941 tgttgtttag ccttagtgcg ttctctacag gaactattct tggatggcaa ttttacaata
    24001 atgaacactc tcagatagtt gcccatgctc aatcagctca aaaaaagttg atactgacta
    24061 ccaatgaaca acagctcgtt gaaaaaatcg atcaactatt aagaaaaaat gattatattg
    24121 gctcaattta cgtaaagaaa aataatcgca tcatcatgga aaaaggctat ggctatgcga
    24181 ataaactcac gcaacaacca aatgatcctt ctctttatta tcagattggt tcggttcaaa
    24241 aagcattgac tactcttttg attttaaaac aagtcgagcg tgggcacctt tctttagata
    24301 ctaagctggc tcaattctat ccacaaattg ctggcagcca gcaaattact atccgtgact
    24361 tgctgtatat gcgctcaggc ttgcaccgaa ctgcttctcc aaccataccg atgactgatg
    24421 aagaagtcgt tcaatttgcg attcagcact taaaatttac agattatcag acctctcatt
    24481 atgattcatt gaactttacc atattgactg gaattttgat caaattaaca aatcaaccgt
    24541 atgaaaaatt attgaccgat gatatcatcg aacctttagg attacagcat acaaaatttt
    24601 atgaccaagt caaaaattca tccaatcatg cactatccta tcaaatgtct aaagaggatg
    24661 attactttaa accactgaca gaatctgaaa cagagatcca taacgaatta gggactggta
    24721 atatcagtat gtccgtctat gatttaaata cgctgtttac acaagtttta tctggaaaac
    24781 tagtttccaa agacttactc ttctcattat ggcgtgaaaa tgcgaatgga catccatata
    24841 gtggtggggt atatagcgga gataattata tcctgtcgca aggaaatatc gactcttttg
    24901 atacatctgc tatatttagc aaagattcac aggatgctat catcatggaa agcaatataa
    24961 aagctgacaa aactatcaaa ctgcctactg ctgatctaag aaaccaaatc tatgacctga
    25021 tggaaggaac tactaagttg gcaaatagct gatatgatga aaagatagca aatactactt
    25081 ctttgaatag catttgctta aaaaaagtac tcgactaaac gtaggaaaga gtgtgggaca
    25141 aatctaaaaa tcaatttttg ctacagactt taattccaaa taaacagaac agaaagaggt
    25201 gttcttcttt ctgttctgtc ca
//
LOCUS       DAZKOW010000032        24943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000032 DAZKOW010000000
VERSION     DAZKOW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.032"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..497)
                     /locus_tag="ACG912_001272"
     CDS             complement(<1..497)
                     /locus_tag="ACG912_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFQ0512605.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
     gene            complement(738..3083)
                     /locus_tag="ACG912_001273"
     CDS             complement(738..3083)
                     /locus_tag="ACG912_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFQ0512606.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            3362..4264
                     /locus_tag="ACG912_001274"
     CDS             3362..4264
                     /locus_tag="ACG912_001274"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFQ0512607.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            4475..6250
                     /locus_tag="ACG912_001275"
     CDS             4475..6250
                     /locus_tag="ACG912_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFQ0512608.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(6316..7902)
                     /locus_tag="ACG912_001276"
     CDS             complement(6316..7902)
                     /locus_tag="ACG912_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFQ0512609.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            8153..8377
                     /locus_tag="ACG912_001277"
     CDS             8153..8377
                     /locus_tag="ACG912_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFQ0512610.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            8519..10270
                     /locus_tag="ACG912_001278"
     CDS             8519..10270
                     /locus_tag="ACG912_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFQ0512611.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            10270..12054
                     /locus_tag="ACG912_001279"
     CDS             10270..12054
                     /locus_tag="ACG912_001279"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512612.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            12389..13241
                     /gene="licT"
                     /locus_tag="ACG912_001280"
                     /pseudo
     CDS             12389..13241
                     /gene="licT"
                     /locus_tag="ACG912_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            13338..15224
                     /locus_tag="ACG912_001281"
     CDS             13338..15224
                     /locus_tag="ACG912_001281"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0512613.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(15265..16167)
                     /locus_tag="ACG912_001282"
     CDS             complement(15265..16167)
                     /locus_tag="ACG912_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0512614.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            16397..17800
                     /locus_tag="ACG912_001283"
     CDS             16397..17800
                     /locus_tag="ACG912_001283"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0512615.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17800..19233
                     /locus_tag="ACG912_001284"
     CDS             17800..19233
                     /locus_tag="ACG912_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFQ0512616.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            19378..20304
                     /gene="trxB"
                     /locus_tag="ACG912_001285"
     CDS             19378..20304
                     /gene="trxB"
                     /locus_tag="ACG912_001285"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFQ0512617.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            20517..22244
                     /locus_tag="ACG912_001286"
     CDS             20517..22244
                     /locus_tag="ACG912_001286"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFQ0512618.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            22260..22610
                     /locus_tag="ACG912_001287"
     CDS             22260..22610
                     /locus_tag="ACG912_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFQ0512619.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22704..23564)
                     /locus_tag="ACG912_001288"
     CDS             complement(22704..23564)
                     /locus_tag="ACG912_001288"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFQ0512620.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23656..24084
                     /locus_tag="ACG912_001289"
     CDS             23656..24084
                     /locus_tag="ACG912_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0512621.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            24246..24422
                     /gene="rpsU"
                     /locus_tag="ACG912_001290"
     CDS             24246..24422
                     /gene="rpsU"
                     /locus_tag="ACG912_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFQ0512622.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            24449..24895
                     /locus_tag="ACG912_001291"
     CDS             24449..24895
                     /locus_tag="ACG912_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFQ0512623.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7878 a         4452 c         5293 g         7320 t
ORIGIN      
        1 taagtagcgt ccataaaaat agcgacatac ttatcattta aggctctgga tttaaaggca
       61 tttacttctt cagtcagaac tttagtcatg ttggacatgg tttgtggagt atagtgatga
      121 ccgtacattt tttcgatcaa atcagcaatt tcagacatcg taacaccttt ttcgaataaa
      181 tggataatag tggtttccaa tgtatcgttt gttcttttgt aggctggtaa agtttgttgt
      241 ttaaactcac cattacgatc tctaggtatt tccaatgtta attcaccata ttcggttttg
      301 attgatcgaa agtaagaacc gtttctcgaa ttacctgaat taaaaccagt gcgatcatat
      361 ttttcgtaat ctaaaaaagc cgttaattca gtccgtagga gtgtgtttat cgctttttct
      421 aagtgcgaac ggaataattc atttaaatcg cctttagtga ctagagtttg cacaatttct
      481 gtagtaaaat cattcatagg gaagtcctct tttctgtgaa ttggttgtcg ttaactttat
      541 tctacagaag ggacttcctt ttttgtatgg attttttcat ttacacaaaa tattttatac
      601 tctctggttt ttcacatttt tacataaatt tgtcttaata tcgtagatgt aagcttttga
      661 acaccattta ttcggaaaaa gggagctgtg acaagatttt tgtcgcagct ccctttaatt
      721 ctgattaatc tattagatca atcatcttta tcgtctgatt ctttggtctc tggattcgct
      781 ctagcataag ctgaagcggt gttccagtaa tctgtatagt tttcatcagt accgcctacg
      841 ccgaatttaa aggcgctttt cttcggacca ttttttgccc aaagtgactc cactgttttt
      901 cctggcgttt ggatggattt tttattgaca gtaacttttc cagctgtttg tcctgtgaac
      961 ccagatactt ttttcttcat cacatcggat gataagtcaa atttctcatc agtgccgaaa
     1021 atctcagggt ttgcttggta agcttgattg attagattgg caagataatt agccgaacgt
     1081 attcttgctt ggctgtccat tccagtattg tcatcgtgtc ctgcccaact actgatagtg
     1141 atagatggag ttgagacgat cagccatgaa tctcggtatt cattggtaga accagttttt
     1201 ccgacccaat ctgcatttcc tagatttccg tttaagcttg agatgacatc tttgaatggt
     1261 gtagtgattt tttcattgat cacactacgc atcatgtcat tcatgatcga tgccgttgct
     1321 tcggaataaa tacggacaga attactttca tgtttataaa tcacattacc tgcattatct
     1381 gtgatggaat caatgatata gccttcttcg tagactccac catttgctaa ggcctggaat
     1441 ccatttgtct gttgaagcgt cgttacatct actgtcccta aaggagccga ttcaacgccc
     1501 caattatcat ttgctgggta gttcatcttc gccaagtagt gctcgtaagc gtattgtttc
     1561 gatccaccgg aattcaacac atcttgatat aagtgataag ctgggatatt gttggaccat
     1621 tccagtgact cacggacggt ttggaaggtg ttcgaccctt cattggtcgc attgacgatt
     1681 ttctcaccag cattttttcc ttcttgccaa gtcgttgcat aatctgatac acgggattct
     1741 gatccgacaa gtcccatatc gattgcagga ccgtaaacaa gaaccggttt gatggatgaa
     1801 ccagcttgac gttctgtatc aaaggcgtgg ttattttgat tctggctgta attccgacca
     1861 cccacgaagc catagatacg tccggttctg ttatccatca atacatttcc agtctccact
     1921 tggctaccag caccatcatc taacatgtag ccataatttt ctacgccggt ttgtaatgca
     1981 gcatagatat ctttatcgat cgtcgaatgg atcgtatatc ctttgttttg gatcgtttgc
     2041 tgtgcttttt gataataagc ttcactgacc gatgaatctg acatatcagc attatctttt
     2101 tctgccaatt gttgtgcaat aatccttgtt gcttcgttca tcacggtata atacaagaat
     2161 tctctgtcag tctgttccgc gatttgttgt gggagaaagt cttttgtaag atcataggct
     2221 ttcgcttctt cgtattgttc tttcgtaatt tgtccttcac ggtacatact gaataaaaca
     2281 aaatcttttc tggcaagacc ggcagaaaga tcttctttta gcgcgcctgt attcgtgtat
     2341 ggactatacg tgatagggct ttgaggcaaa ccagcgatat aagcagcttg tggcagagtc
     2401 acatcctttg catctacacc aaagattcct ctagcagctt cctggactcc agcaatattt
     2461 tgccccttat tattccgacc aaatggtgag acattcaaat acgtagtaat gatctcattt
     2521 tttgaaaagt gtttttctac ttgtgcagaa agaacgatct cgtttgcttt acgtttgaat
     2581 gttgtttcat cggttaggat ctgttgtttg actaattgct gtgtcaaagt ggatcctcca
     2641 gacgagccga tgccagtcgc ttctgaaact agtgctcgta aaacagcttt agggacataa
     2701 ccttgatgtt tatcgaagta ttcatcttct gtactgataa tcgcggtttt caataaaggc
     2761 gaaatcttat cagatgaaat agtcgtccgc atcagatctg tctggatttt tgatatctct
     2821 gtattatctg cataaacaat ctttgacgtt tccgtaatgt ttcctagttc tgtctgcagt
     2881 tcttttttag tcggcaattg tgtgtcttcg acaagatagg caaagtagcc agcacctatt
     2941 ccgataccta gagctgcgaa caacaaaaga agaacagtaa taaaaacagt caatgattga
     3001 aacactctaa aaataatctt aggaacaaac catttttctt tttttcttct attctgtttt
     3061 tttcgagtat ttttgcgtgg caaaagggtc acctcaacta atggttttat ttcgctaatt
     3121 atagcaaata ataggacaaa aaaacagaca aggctgtttt tgacaggaat ttctttttcg
     3181 aatgagataa aacgtgagga aatgatcttt tttatgaaag agttcttaag cttttcttta
     3241 gggaaagttt cagttactat ggagaacagg gcaaagaaaa acaaaccatt ttctttgtac
     3301 atcatcttat caatatacag gaattgttcg aaacaattgc ctttcaagga ggaatccatc
     3361 catgttatta ggctcacatg tcagcatgag cggaaagaaa atgctgttag gcgcggcaga
     3421 agaagcagcc agttacggat cttcgacctt tatgatctat acaggcgctc ctcaaaatac
     3481 tagaagaaaa gctatcgaag agatgaatat tgatgccgga aaagcattta tggcagaaaa
     3541 cggactaaca aatatcgtcg ttcatgcgcc ttatatcatt aatttgggaa atacgatcaa
     3601 accagaaatg tttccatttg ctgtacaatt tctgagagaa gagattgtcc gtgcggaagc
     3661 tttaggtgct actcaaatca cccttcaccc aggcgctcat gtaggagcag gggcagaagc
     3721 tgggattgct agaattattg aggggttgaa tgaagttttg acaaaagaac agacggcaca
     3781 gatcgctttg gagacgatgg cagggaaagg tacggaaatc ggtcgaacat ttgaggaatt
     3841 agcgcaaatc atcgatggag taaaattgaa tgaaaaatta tccgttactt ttgatacatg
     3901 tcatacgaat gatgccggat ataatataaa agaagatttt tcaggtgtaa tggaagagtt
     3961 tgataaggtc atcggattag atcggcttaa agtactgcat atcaatgatt ctaaaaatcc
     4021 acaaggttct cataaagatc ggcatgccaa tatcggtttt ggaacaattg gatttgacac
     4081 attgaacaaa atcgtccatg atccaatgtt tgacaacatt tcgaaaatat tagaaacccc
     4141 atatgtcggt aaagataaaa agaaagctta cgcaccatat ggaaaagaaa tttccatgtt
     4201 caaagcgcaa gcattcgatc ccgaagtgtt ccctgagtta ttagcagaag aagtcacact
     4261 ttaagttttc tacctgacag caacaaaaaa ggattgggca gtctgccttg tccttttttg
     4321 tttaagaaac aaaggagtgc tttttgcatt cgttttcgcc atgctccttt tgccccctct
     4381 ttttaaattc agacaaacag ggtatactga tagaaaagta aaaaacgaaa agtttcacaa
     4441 acaagtagca aaacgtgatt gaaaggacga aaagatgctg aacattttaa accccttgat
     4501 cgaccaacta aaaaagaaac atacatactt tattcttggc atagccgtat tatttacaat
     4561 tattcggttg tatttatata gtaaagcaat ctatggaaca gatatgtcgg cgacctatga
     4621 tgatcagctg ctgattcact atgctcaaaa tattttggat ggtaaatggc taggggagta
     4681 tacgactaca acactttcca aaggaatctc ctacagctta tttttagtac ttgcacataa
     4741 actgcatctt tcttatgcag ttttgttcgg catgttcagt atttttgcca gtatattggt
     4801 tgcactcgcg tttaaaccga ttgtgaagaa caagtatctt cttttaagta tctattttta
     4861 ttttttgttt tctccgatta gttttacaca tgaatattcc actcgaacat atcgtaatgc
     4921 cctcgtgatt ccggctgttg agatcattat cgcttgttta ttagctattt attaccgcaa
     4981 atacggttct agcagaaaat tgctgccatg gtttttaggg ctttctgtcg cttttccttt
     5041 cttttggttc attcgcgaag attctatctg gcttcttcct tttactgtag tagctttggg
     5101 tatcagtttc gtccagatct tgctagggca agacttttct ttcaaagaag taaagaagtt
     5161 aaaaaaacga ctctttcttc tctcgaaacg ggaatggctg aaactttcat tgtttttact
     5221 tcctatagtt ggcttttcca tcgtctatca gtggatcgaa tataaaaatg agacaacata
     5281 cggtattgca accatcaatg accgttccag tggagaattt ggacgtttga tgaaacaact
     5341 gatccgaata gatgatgaaa cgaacttgaa taaagaaaac agtgagatat gggtctcaca
     5401 tgctgcacta aataaagcat tagccgcatc tcctacattc catactgttt ctacccgtat
     5461 tgacgaatta tatacgactc acgcgtggac tcaaggtgga aaagtaaaag aattacccgg
     5521 agatatcatc ttttgggcat tgcgagatgt ggtaagcgaa tctggttatt accaaaacaa
     5581 tgcaaaagaa accaatgatt tttggaaaaa agtcaaccaa gaattagaag aagcctatca
     5641 caagggaacc atcaaaaaga aaaaagagtt ctatctgatg ggatcaggag atggaaagac
     5701 gatgagtgat ctgccaatcg tattcgattt ccttcaatca ggatacgctt atgcatggcg
     5761 atataaagat ttccatcaag gaggaaatta tagtttcgga acgcaagaac aagtcaaaca
     5821 ggctcaagaa ttattaaacc ttccgctaat ggagaattgg atcgatagta atgaaccgag
     5881 aacacctaat ttccaattaa caaaatcagc aaaagtcgcg aatgtcatca taaaaattta
     5941 ccaagttctg gctattcccg tgctgattgc ttcaatagca gggatattac ttatgatact
     6001 aggaaccgta tttgcaaaag ctgatcgtgc ctattatagt tcctctttga ttgtggtcac
     6061 cggattaata ctgacacaat tggtttttct tttcggtgtc tcatggtttt gttcttatgc
     6121 gccagaaaaa aaggactatt ttttaagtgt ctacactggt gcaggcgtgc cattaacgca
     6181 attcatcgta gtaacaagca tgctagtttt cagtaaatta aatctgaaaa aagctttatc
     6241 ttctaaataa aaagaccctt ggtagctaga tttttaacct aactatcaag ggtctttgtg
     6301 tggaaaaatc agtttttatt cgatttcttc taccgtttcc tgcaaaagct gttcatattt
     6361 tttgtttcct ttatataaat caccaatcgt ccatgccaac aggagaagga caagaacaat
     6421 gattacataa gcgatgtgat cagccaatgc tagttgagcg gaagtggctt gctcgccgaa
     6481 gaaactttcg atctgtcctg gtaatcctcg taagttcaag taagttagac taatcacaga
     6541 gaaccagcca agaagtttta cgatgaatga atttttaaat cggctaccca tctccgcttg
     6601 gctgttggtc atcatcagta aaggaatcat cgaaaatggc aacgcaaaag ccaagaacac
     6661 ctgggattca ttcattaagt tattcaaagc ggcatgttct tcgatcgttc cttttgtact
     6721 tgtaaacaaa acacaaatca atacaggaat cacagaaagc aatcgtgtga ccagtcttct
     6781 tagccaaatc gggatacgca tatggatgaa tccttccatg atgacttgac cggtcaacgt
     6841 accagtaata gtagagttct ggccagaagc aagaagtgct acagcaaata aagtggacaa
     6901 gataccagat tttgctacag caatcaaaat accattgctc atagctgatg gatcagataa
     6961 agcttcatac agaccaaaaa aggaaggatc ttggacagaa ccggttttga agacagcgac
     7021 acccatgatc aatagcagtg cattgactag aaaagccatt gacaactgga tattagaatc
     7081 ccaagtagaa aaacgtacag cttgtgctac atcttcttcg ttttggtgat cgatcttccg
     7141 agtttgtgaa actgctgaat gaagatataa gttatgcggc atgactgttg caccaataat
     7201 ccctaaagct ccagtcagcg gagtttcgcc gccgatgacc ggtgagtctg agaatgtatg
     7261 actgttaggg ataaagccag caaataaact gtgccaatct gggttagaca agccaacttg
     7321 atagacaaaa acgaataaaa tgacgaaaat taagcagaca accagtgctt cgatcttacg
     7381 aaacccgact tttgtcagta gtaagagaac gaaaacatct aatacagtaa taaagacggc
     7441 aatcaccaat ggaatatcaa aaagtaagta gagggcgatt gctgcaccaa tgacttctgc
     7501 gatgtctgtt gccataatgg ctaattcagt caagatccat aaaatgacac ccaatgtctt
     7561 acttgtccgt gctcggattg cttgggccaa atccatttgg gtcacgattc ctaattttgc
     7621 tgccatatac tgtaaaagca tagcaattag actagacatc aaaataactg acatcaataa
     7681 atattggaaa ttttgtccac cagtgatcga agtggaccag ttacctggat ccatataacc
     7741 aactgctact aatgcacctg gtccagagta agcaagaagt gttttaaaaa aactttttcc
     7801 ctttggtaca tccactgttc catttacttc agctaaagat aaaccatttg tatgttcaat
     7861 aagtcgatgc ttatgaaatt gtttgttgga ttgattattc aagaaaaaac cacctttcct
     7921 atttatctac gattacattc tacacctcag aaagaaaaaa ttaaagaaaa agattcggta
     7981 tgcctaattt tttttcgtgg tgtgtcttgc tggttaatga aagaaatttt gttcttctct
     8041 aaaaaaacca ctattttttg catgaaacta aagaatacgc ctttcttttt tcaataaact
     8101 ttagtaaaat aaatcattga gagcaattcg gaaatagaaa ggagagattt atatggtatc
     8161 aacaggtatt gtcgtactta ttgctattat tgctttgcta atcggcgcag taggtggctt
     8221 tttcctagcg cgtaaatata tgaaagacta tttagaaaaa aatcctcccg ttaatgagga
     8281 catgttgcga tcaatgatga tgtcaatggg gcaaaaacca tctgagaaga aaattcgcca
     8341 gatgatgcag caaatgaaga accaagggaa aaaataggtt ctgacccaaa gactttttgc
     8401 gggctgattt tttcagtccg ttttttattt cgtaatttgt tagactattc agactattca
     8461 gcaaaaaaga caagcacaga caatcctatg ctcttgataa agacaaagga ggagtaaaat
     8521 gtcgattttt agaaaattag gttggttttt tcgtcaggaa aagaaacatt atttaattgg
     8581 gattttctcc ctattcgcag tggccttggt acagttagtt cctccaaaag tgattgggat
     8641 catcattgat gagatcgccg ataagaattt atcaataaag atcatttcct ggtggatctt
     8701 attattgatc cttgcagctt tcttacaata tctttttcgt tatatctggc ggacgaatat
     8761 ttggggaagt gcagctcggt tggagagaga acttcggcag cagctattca aacattttac
     8821 agaaatggac aatctctttt accaaaaata tcggactgga gatttgatgg ctcatgcaac
     8881 gaatgatttg aatgcaatcc agaatgtagc aggagctgga atcttgacct ttgcagattc
     8941 agtgattacg ggcggaacaa cgatcattgc gatgattttg tttattgatt ggcgcttaac
     9001 actgattgcg ctgattcctc tgcctttgct agccgttact tcacgagtgc tggggtcaaa
     9061 actccacgat gcttttcgtg attcgcaaga agctttttct agtatcaatg ataagacaca
     9121 agaaagtatc actgggatca aagtcattaa aacatttgga caagaaaaag aagatctcgc
     9181 ggattttgaa gaaaagattg aagatgcaat tattaaaaac aaacgagtca attttttaga
     9241 ctccttattt gatccattca ttaccttgat tatcggtctg tcttatgttg caacaattat
     9301 tttaggtgga aagtatgtct tggaaggcac gatctcaatc gggcagatgg tctcatttat
     9361 tagctatatc ggtatgcttg tttggccgat gtttgcgatt ggtcgattgt tcaacgtatt
     9421 ggaaagagga aatgcttctt atgatcgcgt caatgaactt ttgcatgaaa agacgcatat
     9481 cgtggaacaa ctgggtgcag tgcagacccc ggcaaaaggc gagctgaatg tagctatttc
     9541 ctcttttaac tatccgaaag aagaatcacc agcattagaa caaatcaaat ttgcgatcca
     9601 agaaggagaa acattaggta ttgttgggaa aactggtgcg ggaaaaacga caatcatcaa
     9661 attactgatg cgtgaatacg atgattatca agggaaaatc caatttggcg gacgttcgat
     9721 caaagaatat tcattgaatg ctttattagg atcaatcggt tatgtgcctc aggatcactt
     9781 cctgttttcg atgaccattc gagataatat ccgtttttct aatccaaaac tttcagaaga
     9841 agaagtagag catgcggctt ctttagcatt tatcaacaat gaaatcaagg aattgccaaa
     9901 aggttatgat accttagttg gagaacgtgg agtctcttta tctggcggac agaaacagag
     9961 gatttcgatt gctcgagcat tgatcaaaaa tcctgaattg ctgatcttag atgatgcact
    10021 ttctgcagta gatgccaaaa cagaagaagc tatcttgcat aatttaaaag aaataagaaa
    10081 agagaaaaca accatcattg cagctcaccg tttaagcagc gtgatgcatg caaaagaaat
    10141 cattgttgtc gatcaaggga aaatcattga acgaggaacc caccacgagc tgcttgcatt
    10201 aggtggctgg tatgaacgaa tgtgggaaaa acagcaatta gaagcgaaga tagaaggagg
    10261 cgaggcctga tggatgaaaa ttatcaatcg gaatggacaa aatcgattcc acttaaagaa
    10321 cagaagaaga tcatcaaacg actgatgaag ttcgccaaac cttttagaag aacgttcatc
    10381 gttgctatct tatttgcgtt tgccctgtct gtgatcaatg tcctattgcc aaggatcatc
    10441 caaactttta tggatgatca tctggctaaa cagtcagcta cgacacaagt tattttgttt
    10501 tttgctggtg tgtacttgtt tggagtcata gtcaaaagta tcatttggtt tttccaatgg
    10561 tatttgtatt ccatggcttc attgaagacc tatcagtatg ttcgggtaag attatttgaa
    10621 aagctgcata cactaggtat gcgttatttt gaccagacac cggcaggatc gaccgtttct
    10681 cgagtgacga atgacacaga aacgctattt gaattttggt atgtcttttt gatggtattg
    10741 actgggatct ttgcagtcgt ttcttctttc gttgccatgt ttcaaatcaa tggagaaatt
    10801 gccttgtgga ctttggtgtt cttgccgatt ttaggaatcg tcatctggta ttaccagaaa
    10861 ttcagttctc gtatttaccg aaatatgcgg gaaaaattaa gtcagctgaa cacgaaattg
    10921 aatgaatcga tttccggcat gcgtattatc cagcagtttc gccaagaagc tcgtttagct
    10981 aaagaatttg aacaagtgaa caacgagtat ttagacacgc gctatgcgat gatcaaaacg
    11041 aattcgattc tgttaagtcc aatcatcaat tttctctatg cattggcgat tgcgttggcg
    11101 ttgactttgt ttgggatcga tgcgttgcat tctcccgtag aagttggact gatctatgca
    11161 tttacgacct atgtccagga attttttaat ccaatgtctc aaatgatgga ctttttgagc
    11221 acctttacag atgggatggt tgccggtagt cgtattttga aaatcatgga tacagaagaa
    11281 tatgcaccgc aacaaaatcc tggagcagat caagcgatta cttcaggtaa gatcgagttt
    11341 cgcaatgtaa gcttttctta tgatggagag cacaatgtat tgaacaatat ctcttttgtc
    11401 gctaatccgg gtgagaccgt tgcattagtg gggcacaccg gaagcggaaa aagctcgatc
    11461 atcaacgtat tgatgcggtt ttacgaattt tatgaaggcc aaatcttgat tgatgatcat
    11521 gacattcgag aatatccaat ggaagagtta cgtaagaaaa tgggattggt cttgcaagat
    11581 gccttcttgt tttatggaga tattgcagga aatatccgga tgatgaatcc agctatcaca
    11641 gacgaacaga ttcgccatgc agctgagttt gttcaagcaa acaagttcat cgatgaatta
    11701 ccaaaaggat atcactcaaa agtgatcgaa cacggtgcca gctattctag tggacaacga
    11761 cagctgatca cttttgcgcg aacaatcgtg acagatccta aaatactagt attggatgaa
    11821 gcaacagcaa atatcgatac tgaaaccgaa ggattgatcc aagaaggatt ggcaaaaatg
    11881 cgacaaggac ggacaacgat agcgattgcc catcggctgt ccacgattcg ggatgcagaa
    11941 ttgattttag ttttagacaa aggtcatata gtagagcgtg ggaatcatga aacacttcta
    12001 gcgcaaaatg gtttgtatgc agatatgtac aaactgcaaa gcagtggtgt gtaaaaagag
    12061 gttgtgacag tagcatagta gcattttgcc ctaagcgcta gagtgaaaat aaaacagagc
    12121 ttcgcagatt cttcatctgt gagcgttagt ggcgtagtca cccgatttaa aaaagacgac
    12181 gacagctagt caaaagcggc taataaccac taagaaagcg ttgacaaaag cgtttgcttt
    12241 cgataatata aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg
    12301 atgagtctcc catggttcca tggacggctt tttgggtttt tctttttgtt ttggatacag
    12361 ccctatatat catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt
    12421 cgtaataacc agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt
    12481 caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac
    12541 aaatgacggg ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt
    12601 agaaatttct tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga
    12661 ttctgtgtat attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg
    12721 aatatttgta ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc
    12781 catcgggaaa aaaaacagtg aagaaaatct cggataaata ccacatcaag ttatcagaag
    12841 atgaagccgg atttatcgca ttgcatttag tcaatgcaca aggcattcaa agccaacaag
    12901 acaataccta cctgttcact ataacaatac aggatattct aaatatcgtc cgctattact
    12961 ataagattac atttgatgaa gattcagtcc atttttttcg attcgtcaca cacttacgtt
    13021 tttttctgac acgcgtccta gatggagaaa aatcagaagt gggagaagtt tctgaggaat
    13081 tgtttcaggt tattcaaaaa aaatataagg atacgtttgg ttgcgttgag aaaatagcag
    13141 actttttagc cgagacgttc aactaccaga tgtccacgga cgaaaaaatg tatttaacga
    13201 tccatactgc gcgtttagtg catgagaatc gttgtaaata aataaaaaaa ggaggataaa
    13261 ttaacaagaa cgtatgaaat aagggtagtg acattcagtt ggctaaacct tccactaata
    13321 aattggaggg aatagaaatg ggaaaatacc aagaattagc gagaaaaatc gtagaaaatg
    13381 tcggcgggaa ggaaaatatc aatggtttga cacattgtat cacaaggctt cgtttcaaat
    13441 taaaaaacga agaaaaagca aatgatgaga tcttgaaaaa tatggacggt attgtaacgg
    13501 taatgcgagc gggagggcag tatcaagttg tcatcgggaa tcatgtgcca gtcgtgtttg
    13561 aagaggttat aaaagcagga aaccttactt ttgatgaggc tgtttcaaca gaaaaaatga
    13621 gaccgtttga tatgcttatt gatggaatat cgggctgttt ccaaccattc cttgctatcc
    13681 tagctgctga cgggatgatt cgtggactga cagcattcct tgtcttctta ggcgcgtttg
    13741 accgcggttc tggcacattt gttatgtttg ataatatcgg tgattctgta ttccaattta
    13801 tgcctgttat tatcggttta acagctgccc gtaaatttaa agtaaatgaa ttcgtaggga
    13861 tgttgcttgg cgcagcatta atgaatccta gcttgtcgct ggaagctttg agtggtgcag
    13921 cagaagcacc actaacgacc atattttctg gcactatttt tgaagcacct atttatcaaa
    13981 cagtattcgg cattccctgg attgcgcgta actacgcttc ttctgtcatt cccattatct
    14041 ttattgtgtt gttagctagc caaattcaga aaccaattaa aaagttagta cctgagatga
    14101 tcgcgaattt cttcgttcca ttcttcaccg ttcttattac aatgccttta ggttttttac
    14161 tcgttggacc agtattcaca ttcgcaactg atattttaat ggctggattt gaaacacttt
    14221 tagcactttc gccagttatt tatggagcaa tcgtaggctt cttctggcaa attctggtga
    14281 tgtttggtct ccattgggca atcgtaccaa tgggattgat gcaattttct gtgaatggtt
    14341 ggcaaaatat tatgacacct gttgcagtag tgtcttttgg acaaactgca gcacttaccg
    14401 cactctactt taagcttaga aatccaaaag acaaagcaat tgcaatacca gccattgttt
    14461 ctggtattgt ggggattaca gagccagcta tttatgggtt tacattgcca cgtaaaaaaa
    14521 tatttatttt tacctgtgtc ggtggtgcgt tagggggcgc gtatagtggg ttaatgaatt
    14581 taacttcttg gaatcaaggt ggactaggca tctttacaat tcccaactat attagaccag
    14641 atggtgattt aacagatgtg attaatgtat tgattgggat cgccattgcg atggtggtat
    14701 cctttgcgct gacttttttc ttttggaagg aggaagcaga tgaaacagat gatagacaga
    14761 aaagatcagg taaagaaatt gtaaaaacac cgatacaagg acaaatcgct ccactgaatg
    14821 cagcaaagga tgcagctttt gcacaaggaa cactaggtcg aggaatcttg atctaccctg
    14881 aaaaaggaga agtacgtgcc ccttttgacg gaacagtcat gtccctgttt ccgacgaaac
    14941 atgcaatcgg gatggtttct gaagcaggtc tggaattgct gattcatgtc ggattggata
    15001 ctgttcagtt ggaagggaaa tattttgaaa gcttggtgca gcaaggagaa agagtgaaac
    15061 aaggcgatct gttgcttcgc ttcgacaaaa agaaaatcga agaggaagga tacagtttag
    15121 aaacacctgt gattgtcatc aattacatgg attatttgga tattttggaa aatcaagggg
    15181 aaacggtcgg gccagacgat gcacttatca ccgcactgac ctgaacggtt aggtgaaaga
    15241 atggactagg cttaactatt ctttttagtc cacttcttta ttttcaaaat tgaaaggctc
    15301 tttcatgatg gctgagctga tagggcgttt atcaaatttt tgtcctacag aaattagatg
    15361 gactaaattc tgttgataat tttctgcaaa aacacaacta tataacacgt ctagaagata
    15421 acaaatcgat gtattgatcg taaaattacc aattttcgaa tacagtcgtt cgcgtgtcgt
    15481 aagtcgcaaa gcggctgtac tcattgaaga tagcgttgaa tcaccgatac tggtcaaagc
    15541 taaaattggc acatgatttt gtttcaatat ttctgcggta cgaaggatca cattgttttc
    15601 acccgtataa gaaatcagaa tcccacacat atcacttgtg caattgtacg cttcatagaa
    15661 cgattcacca aaagtggtga caatcgtgac atccattttg atgcgcttca ttttcagcat
    15721 gaaatcttga gaaatcaaag tattcgagtt actggcgaaa atcacgattt tttttgcttg
    15781 aagcattagt tgtttggctt tttgcagttg gtcatgatga atgagagaaa gtgtatcttg
    15841 aatcgtcatc tgttctaaag aagccatttt atttgcgatc gtcataatac cgtctgtggt
    15901 ggaaaaagga agattcgcat ctacgtcttg aaaattccct tcaagatagg ctgtttcagc
    15961 taaaaaagca ttacgaaaac tgacccagcc atcaaaaccc attttttttg ctacacggat
    16021 gagtgtggac gggtggacat aacatcgctc agcaatctct tggatggtca tttcgccaag
    16081 tgctggagaa tcgcttaaga gaaactgaat cacttcattt tctgaaggag aaaaggagat
    16141 ttctttgatt ttttcacgta ataacatgta gataccttct tcttgttttt ttacgtaaat
    16201 cataactttt ttgaaaatga tttgtaaacg gatttatgtt atatagctta ataaagacat
    16261 tttttgacaa gatatttaca aaaatccaga agaaaacact aacttttgta aatgttactg
    16321 gaaagaaacg aatttctatt gtttgttttg tttacaaaaa agataataaa agtgtaaaaa
    16381 gtaaaggagt gagaacatgg caaaaggaat caaaatcgct acgattggtg gaggcagcag
    16441 ttacaccccc gagttgatgg aaggatttat caaaagatat gaggaattac cgatacgaga
    16501 aatctggctc gtagatgtag aagaaggaaa agaaaagctt gagatcgtca gtcagatgtc
    16561 tcagcgtatg tgggatgctt ctccgtatga agtcaaaatc tatgcaactc tggatcggga
    16621 agaagcattg aaagatgcgg attttgttac gactcagttt cgtgtcggtc aattagatgc
    16681 acgaatcaaa gacgagcgga tccctagtta ttatgggatg attgggcaag aaacaaatgg
    16741 tgctggcggg atattcaaag cttttcgtac cgttccagtg gttttggcaa tcgttgaaga
    16801 catgaagcgt ttatgtccaa atgcttggct gattaatttc gcgaacccaa gcgggatggt
    16861 gacagaagca gttatcagat atggcaaatg ggataaagtg atcggattat gcaatgtgcc
    16921 agtaatggcg atgatgacag aaccaacaat gcttggcaag aaaaaagaag agctgatcta
    16981 caagtttgcc ggtctaaatc acttccattg gcacaaagta gcagataata aaggaaatga
    17041 ccgcacggca gaattgattg atcgtttata cgatgaagac aatggattgc cgaaaaatat
    17101 ctttgacatc ccgtttttca aagaacaatt aaaacaaatg aacatgatcc cttgtggcta
    17161 ccaccgctat tactatcgtg aagaagaaat gctggaacat gctttagaag aatatcaaac
    17221 gattggtaca agagcgcagc aagtgaaaga aacagaagca gagctgtttg aattatacaa
    17281 agatccagaa ttggatcata aacctgaaca attgcaaaaa cgaggtggtg cttattattc
    17341 agatgctgcc tgtgaaacga ttgcttcgat ctatggaaat aaagaaacac agatcgtggt
    17401 atctacaaag aatgatggag ctgttcctga tctgccggca gattgtgtag tagaagtcac
    17461 tgcctatgtt ggcgcacaag gcgcacgaaa tgtagcgttt ggttcccttc ctgctgctga
    17521 aagaggctgg ttgcaagtaa tgaagaatat ggaattgctg acgatcgaag cagccgtgac
    17581 tggtgattat ggaacagctt tgcaagcgtt tacgatcaat ccgcttatac gatcaggaaa
    17641 gacagcaaaa cggatcatgg atgagttgtt tattgcacat cagcagtatt taccgcaatt
    17701 caaggaaaca atcagccggt taaaagcaga aggaatcgaa gtccaagatg aactggcaag
    17761 agaattatca agcaatacac atgtaatgga ggaagtatga tgggattcag aaaagatttt
    17821 ttatggggcg gagctacagc agccaatcaa tgcgagggcg gttatgatga aggcggacgt
    17881 gggttagcga acgtagatgt tgtgccaatc ggagaagacc gttttcctgt cattactgga
    17941 aaaaagaaaa tgtttgaatt tgatgaaaat catttttatc cagcaaaaat tgcgatcgat
    18001 atgtatcatc attataagga agatattgct ttatttgcag aaatggggtt caagacttat
    18061 cggatgtcca ttgcttggag tcgtattttt ccaatgggcg atgaaaaaga gccaaatgaa
    18121 tctgggttga aattttatga ggatgtcttt aaagaatgtc aaaagtacgg gatcgagccg
    18181 cttgtgacga ttacacattt tgattgtccg atgcatttga tcaaagaata tggtgcgtgg
    18241 cgttccagag aactcgttgg tttttatgaa aatctgtgcc gagtcatctt caatcgctat
    18301 aaaggacttg tcaaatattg gttgacattc aatgagatca atatgatcct tcatgcacca
    18361 ttcatgggcg cagggctgta ttttgaagaa ggagaaaatg aagaacaaat caaatatcaa
    18421 gcggcacatc atgagttggt tgcaagtgcc atcgcaacga agatcgctca tgaagtagat
    18481 ccggagaaca aagtaggctg tatgctggca gcaggagcat attatccata cacttgcaat
    18541 cctgaagatg tttgggcttc tagaaagtca gatcgagaaa attatttctt cattgatgtc
    18601 cagtcacgag gagaatatcc cgcttatgca ttgaaagaaa tggaacgaaa tggcattcag
    18661 atcaaaatgg aacctgggga tgccgcattg ctgaaagaac atactgttga ttttatctcc
    18721 ttttcttatt attcttctcg agtttcgact actgacaacg aactcttgga acagactgca
    18781 ggaaatatct ttgcttctgt caaaaatcca tatctggaag caagtgaatg gggttggcaa
    18841 attgatccat taggcttgag aataacgatg aatgatattt atgatcgtta tcaaaaacca
    18901 ctatttattg tagaaaacgg cttaggtgca gtcgatacgc ccgatgagaa tggttatgtg
    18961 gaagatgatt atcgaatcga ctatctggca gcacatatcc aggcaatgaa agatgcagta
    19021 gaattagatg gagtcgatct attaggctat acgacttggg gatgtattga tttagtatca
    19081 gctggtacag gtgagatgaa gaaaaggtac ggattcattt atgtcgatcg tgacaacgaa
    19141 ggaaatggca cattagcccg ttcgaagaag aaatcattca actggtataa aaaagtaatt
    19201 gattcaaatg gagaaaatct ttcgaacgat tgatcaatga tttataaaag tctattgttt
    19261 ttcagcggat ttttcgagta taataaagct attcgagaaa cgcaagcaga acaatagact
    19321 ttttttcgca ttggctgtaa agtatgctta cttgattaag taaaggagaa tctacatatg
    19381 tatgatgtaa tcgttattgg cgcagggcct gctgggatga ctgctgcttt atatgcttca
    19441 cgttcaaact tatccgtatt gatgatcgaa caaggcgctc ctggcggcca aatgaacaat
    19501 acggcagaaa tcgaaaacta tcctggtttc gattcaatca tgggtcctga tctagcttat
    19561 aagatgtatg aaggtgtttc aagatttggg acagaaaatg cttatggaat cgtgcaagat
    19621 atcaaggatc acggcagcca caaagaagtt atttgtgaag ataaaactta cgaaggaaaa
    19681 acagtcatta tcgcaacagg ctgtatccac cgtaaattag gcgtacctgg cgaggaagaa
    19741 tttgctggac gtggcgtttc ttactgtgca gtttgtgatg gtgcattttt ccgtaacaaa
    19801 cgtctagtag tgatcggagg aggagactca gcagtagaag aagccatcta tttgacacaa
    19861 tttgcttctg aagtagtcat cgtccaccgt agagatgaat tacgtgcaca aaagatcatc
    19921 caagaccgtg catttgcgaa tgaaaaaatt tcctttatgt gggatacagt cgttgacgaa
    19981 atcgttggaa atgaaatggt agtgacaggt gtgaaagcac gtaatgtcaa aactggcaat
    20041 gagtttgatt tggcagcaga cggagtattc atctatgtag gattggatcc attaactgca
    20101 ccatttagag aagctgggtt gacgaacgaa gccggctgga ttccaacaga caacgaaatg
    20161 aagacgaata taccaggcgt ttttgccatc ggagatgttc gtgaaaaaga tttgcgacaa
    20221 atcactactg ctgttggtga tggtgggatt gctggacaac aagtgtataa atacttggaa
    20281 tctttagaag aaacaactgc ataagtttaa aaagagtgaa agaacggttt caattgttct
    20341 tttactcttt tttttgcgtg aaatctactg aatccgattt atgcgaatac ttgtaaccgt
    20401 gcttgataaa agaatctttt cttgatttaa gcgttttttg gtcctcatac tttcatatct
    20461 aataagtgaa tgctatactt attaagaacc atatttcaga aagaggtgtt attttcatgt
    20521 cttgggaaca agtgtatgaa caatgggtac atgaagaaaa tttagaagaa aacttaaaaa
    20581 aacaattaaa tgatctgggt gaagatcctg aaaaacttga agatgctttt tatgcaccgt
    20641 tggaattcgg tactgcaggc atgcggggaa tcttaggacc tgggatcaat cgaatgaaca
    20701 tctatacggt aagacaagca acagaaggac ttgcacgttt catggataca caaggtgaag
    20761 aaacaaaacg ccgaggtgtc gctatcgctt atgactcacg ccatcaatca ccagaatttg
    20821 ccatggaagc agccaaaaca ttagcacatc acaatatccc ttcttatgta ttcgaaagtt
    20881 tacgtccaac accagagctt tcttttgcag tccgtcattt gaagacgttt gcgggaatca
    20941 tggtaactgc atcccacaat ccagcagctt acaacggata taaagtctac ggagaagatg
    21001 ggggacaaat gcctcctgca gacgcagatg cattgacgaa atatgtccgt gaagtcagca
    21061 atccgttaaa agttgaagta ttatctgacg acgaagctga acatagcgga ttgatcacga
    21121 tcatcggaga agaagtggat gccgcttatc tagaagaaat caaagcagta acgattgatc
    21181 atgaattggt ggaaacaatg ggtaaagatt tgaagcttgt gtacacacca cttcacggaa
    21241 caggaaaaat gttgggcgaa cgtgcgttga aacaagctgg attcgaacaa ttcgtactag
    21301 ttccagaaca agctgtcgca gacccggatt tcactacagt caaatcaccg aatccagaag
    21361 aacactctgc tttcgagtat gcgatgcgac ttggcgagaa agaaggcgct gacttactga
    21421 ttgcaacgga tcctgatgca gaccgtttag gcgcagccgt tcgtttgcca aatggctcct
    21481 atgaagtatt gacaggaaac caaatcggag ccctgtttgc tcaatatatt ttggaagcac
    21541 accaatcgcg aggcacttta cctgaaaatg cagcggtatt gaaatcaatc gtttctagcg
    21601 aattgcctac agcaattgcg gaacactatg gggctaccat gttcaatgta ttgacaggat
    21661 tcaaattcat tgctgaaaaa attcaacaat ttgaagaaga tcattcccat acttttatgt
    21721 tcggattcga agaaagttac ggttatttag ttaaaccttt cgttcgcgac aaagacgcga
    21781 ttcaagcatt gctcttatta gcagaagtag cagcttacta taaaaaacaa ggcaaaacat
    21841 tgtatgacgg cttgcaaacg atttttgaaa catatggcta ctacgcagaa aaaacgattt
    21901 ctgtgacaat gagcggacaa gaaggctctg caaaaatctc tgctttgatg gaaaaattcc
    21961 gtgaagaagc accgacagaa tttgccggag caaaagtcat ccaaacagaa gacttcaaag
    22021 agttgacgag aacagaccgt gaaggaaacg tagaaaaact aacgacaccg ccatctgacg
    22081 tattgaagta tacgctggaa gaaaatggct ggatcgctat ccgtccttca ggaactgagc
    22141 cgaaaatcaa attttatatc ggtgtaaaag gaaactcttc agaagacgca gctgaaaaag
    22201 tagccgctta tgaagcgatt atcaaaaaaa tgacagaaga ataaaaaaac ttgagactga
    22261 tggaaaaagt ggtaaacttt agaagtcagt cttttttcaa gttggaggtg aatggattgc
    22321 cagacacgca aaaaacaaaa gtagagaaag tcagccaact attcaaaatg ttgagtgacc
    22381 ccactcgtct caaaattctg ctgtatctga aagacgggga acaaaatgtg acagcaatca
    22441 cccaagcagt agaaatggaa caatctgctg tatcgcatca actgcgttta ttaagagaaa
    22501 accatgtcgt caagtcccat cgtgagggaa aagcgatttt gtatagtctt gatgattctc
    22561 acgtactgga tattttgaac cagacgctca aacatgtgga acatcaataa cgataggtgc
    22621 aaatagagac aaactggact gtatcaacga atcttcttcg ctgacacagt ccagtttttt
    22681 taagaaaaag acaagaaaaa ctgttagtct gtttcttcgg tggtttcagt tccataataa
    22741 ctatgatctt ctaggtctaa agcattcaaa ataagtgaag ctgtttcttc gatagataaa
    22801 gaagcgacat tgattactac gcagcccaat ttttcataaa gatcattcgc aaaatcgagt
    22861 tcagctcgta ttttttcgat atcggaataa gcagtatcag gatttaatcc gtaagagcgc
    22921 atccgttctt tacggatatt gtttaaaata tctgggttat tcgtcagtcc cacgattttt
    22981 ttaggatcga tctcccaaag ctgtttggga atatgtgctt gtgggatcaa aggtagattg
    23041 gcaaccttta aatttttgtt agctaaaaac aagctcaaag gcgttttgga agttctggat
    23101 acgcctagca aaacgacatc tgcttctaga aacccacgag gatctttccc gtcatcgtat
    23161 tttacagcaa attccattgc tttgatccgt ttgaaatagt tctcattcaa taaatgaagg
    23221 gcgccaggtt gccgaatggg cttgatacca gttaagcgct cgatttctga tatcggcgga
    23281 gtcatcacat caaaactaaa taattgatgt tcttcacaaa aagaacgcgt catctcgatc
    23341 aattcttgat tgatcaatgt gtgtaggaca agggcttttt cttttagtgc atcttgcagt
    23401 gcctgcatca atgttttctc atctgtcaca aatgttctgc ggaataaatt gaactcaacg
    23461 gtaggatatt gggccatagt ggcttgcgct aattttgtcg cagtttctcc agcagagtca
    23521 gagatgacaa aaaaagtaat gatttctgta gaatcggaag acatggataa aacctcactt
    23581 gcattttttt tatgtttatt ataccatact ctagaatgtg tacttcgtaa tcattttgat
    23641 ttggaggaat aagtcatgaa aaaacagaca ttgattgaac aatcactaga aacattgaaa
    23701 aataacggtt ttaaatatac taaaaaaaga gaagcattgt taacttattt agtgaagcgc
    23761 aatcgctacg tatccgctaa agaagttttt gactatatga atcaggaatt caaaggagta
    23821 agttacgata cgatttatcg aaatctccat gattttgcag aaatcgattt actggaagaa
    23881 accgatttaa atggagagat gaagtttcgt tttcattgct gtcaagggga aatcggccat
    23941 caccatcact ttatctgtac ggtgtgcgga acaacgaaag aaatccatat gtgtccaatg
    24001 aattatttcg aagaacagct cggtggatgt acgatcgaag ggcatagatt cgagattttc
    24061 ggaagatgtg aaaattgtca ataaggattt aaataaaata acatttgata attttaaaaa
    24121 aaagcaagat ttccttgacg gtcaaaacag cattagatat aatatttaca ggacagtctt
    24181 gttttttgag atatatttca ataacaatta cggttacttt ttaagctcgg agggagggaa
    24241 ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact tgacgatgct cttcgtcgct
    24301 tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc tcgtaaacgt gaattctacg
    24361 aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc tagaaaacgc aaaaaattct
    24421 agttctgtga tgaattggag ttggtagtgt gtcactacta agtagattaa acgatgacat
    24481 gaaaacagcg atgaaagcga aagataagga atctttacaa gtcatccgaa tgatcaagtc
    24541 atctatacaa aacgagcaga ttaaagaagg tcacgacttg acagaagaag aagaattaac
    24601 ggtgttgtct cgcgaaatga aacagcgacg tgattctttg catgagtttg aagaagcagg
    24661 acgtgacgat ttagctgaaa aagtaaaaag tgaaattgtg atagtcgaaa aatacatgcc
    24721 ggaacaactt tccgacgaag aaattcgaca acttgttcaa gaagcaatca cacaaactgg
    24781 tgcttcatca atgaaagaat ttggcaaagt catgggtgcg atcatgccta aagtcaaagg
    24841 aaaagcagat ggaaaccaag taaatgccat tgtgaaagaa ctattacaat cttaatttta
    24901 tcatttaaag gctcttcgtc aatgagtgtt ggtgaagaaa ttc
//
LOCUS       DAZKOW010000033        24618 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000033 DAZKOW010000000
VERSION     DAZKOW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.033"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            200..748
                     /gene="aac(6')"
                     /locus_tag="ACG912_001292"
     CDS             200..748
                     /gene="aac(6')"
                     /locus_tag="ACG912_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0512624.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            complement(996..1115)
                     /locus_tag="ACG912_001293"
     CDS             complement(996..1115)
                     /locus_tag="ACG912_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0512625.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            1294..2955
                     /locus_tag="ACG912_001294"
     CDS             1294..2955
                     /locus_tag="ACG912_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0512626.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(3025..3372)
                     /locus_tag="ACG912_001295"
     CDS             complement(3025..3372)
                     /locus_tag="ACG912_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFQ0512627.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            3456..4397
                     /gene="opp3b"
                     /locus_tag="ACG912_001296"
     CDS             3456..4397
                     /gene="opp3b"
                     /locus_tag="ACG912_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFQ0512628.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            4398..5441
                     /locus_tag="ACG912_001297"
     CDS             4398..5441
                     /locus_tag="ACG912_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0512629.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            5447..6517
                     /locus_tag="ACG912_001298"
     CDS             5447..6517
                     /locus_tag="ACG912_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512630.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            6507..7460
                     /locus_tag="ACG912_001299"
     CDS             6507..7460
                     /locus_tag="ACG912_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512631.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            7472..8482
                     /locus_tag="ACG912_001300"
     CDS             7472..8482
                     /locus_tag="ACG912_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0512632.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(8767..9588)
                     /locus_tag="ACG912_001301"
     CDS             complement(8767..9588)
                     /locus_tag="ACG912_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFQ0512633.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(9685..9963)
                     /locus_tag="ACG912_001302"
     CDS             complement(9685..9963)
                     /locus_tag="ACG912_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512634.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            10231..12240
                     /gene="metG"
                     /locus_tag="ACG912_001303"
     CDS             10231..12240
                     /gene="metG"
                     /locus_tag="ACG912_001303"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFQ0512635.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(12719..13306)
                     /locus_tag="ACG912_001304"
     CDS             complement(12719..13306)
                     /locus_tag="ACG912_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512636.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            13481..14803
                     /locus_tag="ACG912_001305"
     CDS             13481..14803
                     /locus_tag="ACG912_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0512637.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14958..15734
                     /locus_tag="ACG912_001306"
     CDS             14958..15734
                     /locus_tag="ACG912_001306"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFQ0512638.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            15727..16296
                     /gene="rnmV"
                     /locus_tag="ACG912_001307"
     CDS             15727..16296
                     /gene="rnmV"
                     /locus_tag="ACG912_001307"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFQ0512639.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            16289..17173
                     /gene="rsmA"
                     /locus_tag="ACG912_001308"
     CDS             16289..17173
                     /gene="rsmA"
                     /locus_tag="ACG912_001308"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFQ0512640.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            17624..18253
                     /locus_tag="ACG912_001309"
     CDS             17624..18253
                     /locus_tag="ACG912_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512641.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(18377..19486)
                     /locus_tag="ACG912_001310"
     CDS             complement(18377..19486)
                     /locus_tag="ACG912_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512642.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            19695..20117
                     /gene="mgsA"
                     /locus_tag="ACG912_001311"
     CDS             19695..20117
                     /gene="mgsA"
                     /locus_tag="ACG912_001311"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFQ0512643.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      20304..20402
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            20440..20976
                     /locus_tag="ACG912_001312"
     CDS             20440..20976
                     /locus_tag="ACG912_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFQ0512644.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(21016..22779)
                     /locus_tag="ACG912_001313"
     CDS             complement(21016..22779)
                     /locus_tag="ACG912_001313"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512645.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(22782..24497)
                     /locus_tag="ACG912_001314"
     CDS             complement(22782..24497)
                     /locus_tag="ACG912_001314"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512646.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7956 a         4468 c         5006 g         7188 t
ORIGIN      
        1 agagtttttt cctccaattg atggctttcc tgatctttcc tattttgaat aaagtgtttc
       61 tgtataattt ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg
      121 tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca
      181 agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa
      241 agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc
      301 agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga
      361 tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca
      421 tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta
      481 cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt
      541 agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc
      601 ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat
      661 cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac
      721 gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt
      781 caagaaataa gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa ttccaactta
      841 tttccgaaga agctactttt ggaacaccgt gtaccagagg ttgtgaaaaa gtcgcttagt
      901 ttcgagtagt aagaaaaatt ttgtgaaaat ggctttctac atttttgcat aaatttggct
      961 taataccgag aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact
     1021 tttgtcacag tttctttttg tttattcttt tttatctttg tatggcaata atagacaata
     1081 taacgaaaat tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt
     1141 ttctgaaaaa ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa
     1201 aagtgttgat tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat
     1261 tattctgaaa attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg
     1321 gttgcggtat gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt
     1381 agttcagcaa aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa
     1441 gaaatgccta cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat
     1501 aacgtttatg aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct
     1561 agcgagttgg cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac
     1621 gcaaaatggt caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga
     1681 acagtagacc cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca
     1741 gaagacatta cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac
     1801 tatgaattag aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc
     1861 ccatcatttt tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct
     1921 gcaagtgata aagctgttta taatggacca ttcacattag aagattttga tggaccagga
     1981 acagatacag aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa
     2041 ctttctgaaa tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa
     2101 gatggccaag cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat
     2161 ccagcttata cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa
     2221 aagaattcac cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt
     2281 gaagctttag taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca
     2341 tcagatatgg taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta
     2401 gtttcttatg acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc
     2461 gttgattctt tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc
     2521 gaatatgttc aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct
     2581 gttccattct ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc
     2641 ggatgggctg ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat
     2701 tcatacaacc gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca
     2761 tcaacagacg ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc
     2821 atctctgatg atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat
     2881 gaaaaaatca aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca
     2941 tacgttgaag aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg
     3001 tcgcaacctc gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga
     3061 aagagcaatt agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga
     3121 cgagagcgag gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga
     3181 tcgctgaaaa tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag
     3241 aaacggcaat gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt
     3301 gatgagattt tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga
     3361 tttttttttc atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac
     3421 atctgtcaga agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat
     3481 taaaacgtgt ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct
     3541 tgatgcaatt gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa
     3601 tcgagatgct aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt
     3661 atctttctaa tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag
     3721 tcgcacattt gttagctgga agaatcggac catccttgca gttagggtta caggcaatta
     3781 tctttggaac agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat
     3841 gggcagatac atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat
     3901 ttgctgtttt attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat
     3961 gggatggctt tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg
     4021 actctgctcg gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat
     4081 tagcaaaagc aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata
     4141 gtttgattcc attgatgact ttattaggac cgttagctgt agcattgatg accggatcac
     4201 tggtggtaga aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca
     4261 caacaaatga ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt
     4321 ttgttatttt gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag
     4381 aagggagccg aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc
     4441 ctgcggatga atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg
     4501 caccttcttt gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg
     4561 ttatttcaat ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt
     4621 ctcctcatga tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg
     4681 gaatcaatat cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat
     4741 atgctcaagc aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac
     4801 gtgatgtatt aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg
     4861 ctgcattact agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg
     4921 gacgtgtcga taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg
     4981 ttgtcatgat tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta
     5041 tggccatcac aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa
     5101 aagatcaaga atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat
     5161 tcaaacatat tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc
     5221 catctgctat tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt
     5281 cagcttcatt aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc
     5341 tattatggat tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg
     5401 atggattgcg tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt
     5461 agaagtgaaa gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg
     5521 cggtgtcaat tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag
     5581 tggaaaatca gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga
     5641 tgctggtcaa atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca
     5701 aaaaatccgt ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc
     5761 gacgatgccc attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa
     5821 aaaagaggga caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga
     5881 gaaacgtatc aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat
     5941 cgcgattgca ctggtatgtt atccggaaat actgattgct gatgaaccta caacagcgct
     6001 tgatgtgact attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga
     6061 tacatcaatt atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt
     6121 cgctgtcatg tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa
     6181 cccgcaacat ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa
     6241 cgatcgattg tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga
     6301 cgcattctat ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt
     6361 ttttgaacta tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa
     6421 agtgacaccg ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca
     6481 tacttacggt caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga
     6541 aacagtattt caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc
     6601 acatttatga aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta
     6661 ctggccgtac gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc
     6721 aagatataac gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga
     6781 tgatctttca agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg
     6841 ccgaaggaat tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg
     6901 atgatctatt gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat
     6961 tttcaggtgg gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt
     7021 ttatcatttg tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca
     7081 atttgttgca agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt
     7141 tgtcgatggt taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag
     7201 agatgggaac aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt
     7261 tatctgctat tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc
     7321 aacctgaacc agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg
     7381 tctatgcaac tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat
     7441 ctcttctggt tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta
     7501 tccgacagaa gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta
     7561 taaccatttg ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc
     7621 gctcacaccc gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc
     7681 tttagcaaat gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc
     7741 cagcttccca aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca
     7801 aacccgtacg atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat
     7861 caagataaat cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg
     7921 atataatgaa gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc
     7981 tttagctaaa tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc
     8041 tctagaagta actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg
     8101 gaaattgaat gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag
     8161 caattatcag aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg
     8221 agaagattat cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt
     8281 agaatctttt ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt
     8341 aataacaaaa ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac
     8401 cgacggagca gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag
     8461 acattcggtt atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct
     8521 tgttaataaa aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc
     8581 actaattatg acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct
     8641 attgttaatt ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt
     8701 aactcacaaa gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc
     8761 acaaccttat tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc
     8821 tacaggacgt tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg
     8881 atcgatcgtt ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat
     8941 cgtgaaatct ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac
     9001 acggttgaat gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga
     9061 agactctcca gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt
     9121 gttactatga cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag
     9181 cggttcttct ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg
     9241 attaggcgta ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt
     9301 ttcttccttt gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc
     9361 accaggcaat gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt
     9421 cagctgatct tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt
     9481 gtcatattct ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt
     9541 atgttcataa tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc
     9601 attgacagta aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt
     9661 tacttgaaaa atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa
     9721 taaaaagaaa agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa
     9781 gccacaaata acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa
     9841 actgttcaaa tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa
     9901 tattaaatgt gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg
     9961 catgattgtt ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga
    10021 gaaataaaaa gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac
    10081 ctattcatcc ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt
    10141 cattttgaaa aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt
    10201 cgcatcatgc gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact
    10261 acgccaatct attatccaag cggcaaactt catatcggca actcgtatac tacgattgca
    10321 tgtgacgcaa ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga
    10381 gtggatgaac atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa
    10441 gaatatgtag ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt
    10501 tatgataaat tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt
    10561 gatcgtttat tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt
    10621 tctgacgaag aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt
    10681 aacgtgatcg gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca
    10741 tacttcttcc gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc
    10801 gaattcatcc agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga
    10861 ttagaggatc ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat
    10921 ccgaaacatg tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga
    10981 tatggctccg aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc
    11041 ggtaaagaaa tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat
    11101 ttgcctctac ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg
    11161 tctaaatcaa aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat
    11221 gcactccgtt attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca
    11281 gaagattttg tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat
    11341 cgaacgattg ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt
    11401 gtcactccat ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat
    11461 gatgcaatgg aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct
    11521 cgtgcaaaca aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag
    11581 aaagctgaat tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata
    11641 ttacttcagc caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac
    11701 tctgaaacaa tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta
    11761 gttgcaaaag gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt
    11821 caagaaaaaa tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa
    11881 gaaacagaac ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta
    11941 gaactaaaag tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg
    12001 caattccgac tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa
    12061 ttttatcctg atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa
    12121 ccacgaaaga tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat
    12181 ggctctttac aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag
    12241 aaattcaaag ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag
    12301 caattttcgt aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa
    12361 ctatccaaaa taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga
    12421 ataatactaa taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga
    12481 ttttaattga ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc
    12541 aatgacttta ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt
    12601 tctatcactt tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa
    12661 aaaaacaatc atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc
    12721 acttgatttt atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa
    12781 gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt
    12841 gatagagctt tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta
    12901 taaaccaatg attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga
    12961 aataaaaata gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca
    13021 ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg
    13081 aatacaacat cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga
    13141 acaaagtcag caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa
    13201 ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt
    13261 tggataaata atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct
    13321 tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag
    13381 ggagtttttc actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac
    13441 tgaaaattat accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg
    13501 agaaatcaat ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt
    13561 cttcttcaaa actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc
    13621 ttggcttttg ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac
    13681 agcaatcccg gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc
    13741 cagcttttta ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt
    13801 atcgattatc tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt
    13861 gttgattgca gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt
    13921 cggtgcttct gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt
    13981 catatgggga ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg
    14041 aaaagatgtt acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta
    14101 tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt
    14161 gcctaaagca attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt
    14221 tgtttttttg aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga
    14281 agcatcagac gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat
    14341 ctcggtatac ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat
    14401 ggctttagaa gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac
    14461 ggttccttat gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg
    14521 ttctttcgac tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat
    14581 ttttatcggt gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt
    14641 accactttat ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat
    14701 gacgatgatg acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat
    14761 cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga
    14821 cagaaaaagt acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa
    14881 gttcttctat tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat
    14941 gaaaaggaga gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca
    15001 atgaagatat tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg
    15061 tcgtgggctt cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa
    15121 atatatacag tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg
    15181 aaaataaatt acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct
    15241 tggattatca ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga
    15301 ttgctatcgc aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag
    15361 acacgtatca aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt
    15421 tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta
    15481 gcggtgtcgt cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa
    15541 tcgaacgatt acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa
    15601 agcgaaatga accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc
    15661 ttccgacaga agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa
    15721 gcgaacatgg ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca
    15781 agaaggctgc gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat
    15841 gaagagatcc tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc
    15901 gatcctgatt tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca
    15961 aaacatgctt ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc
    16021 gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt
    16081 tcccaaacgg ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct
    16141 ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg
    16201 aacggcaaac aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc
    16261 caagcaatga aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc
    16321 tagaacaaaa gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa
    16381 ttttctgact gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca
    16441 tacgaacgtg atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca
    16501 tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac
    16561 gatgcaaccc tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc
    16621 aacagctgtt agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc
    16681 ttactatatt acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga
    16741 actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa
    16801 agcttatggt tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat
    16861 cgtaccaaaa acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac
    16921 aagaagagat ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa
    16981 agcggcattt cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa
    17041 agatgaccag acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa
    17101 acgtcgtggg gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga
    17161 aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag
    17221 taataagaac agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac
    17281 cgcagatgca agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc
    17341 taagtcacaa ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta
    17401 ttacttgatg caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt
    17461 gtcacatctc ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca
    17521 ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact
    17581 aagtcgacat ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt
    17641 tcgaaaacta acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat
    17701 gcctaaaatc atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat
    17761 tgcgatgttt tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt
    17821 ctttttaaca acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt
    17881 gattggggct tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac
    17941 ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc
    18001 agaaatgcta gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc
    18061 tcaaggatat ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt
    18121 agttgattat aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc
    18181 cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa
    18241 aacgacatat tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa
    18301 tcctctaaga tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc
    18361 tctaacaggg ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt
    18421 ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg
    18481 catctacttt tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc
    18541 caagtaattc ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg
    18601 ctacttgttc actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat
    18661 aacctttttc aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc
    18721 catctgtgat aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa
    18781 taaagccggc aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt
    18841 gttggataaa tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct
    18901 gatcgtgagt aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag
    18961 cacgcatggc tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga
    19021 cttttgcatc ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata
    19081 aagcagctgg ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca
    19141 cacgtttttt gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat
    19201 caaaaacagt catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt
    19261 ctttaggtgc cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat
    19321 cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa
    19381 aaacgataaa ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg
    19441 cattgtcata ttttttatat acgtttttca gagccatttc taccataata cgttccacac
    19501 tttctttttt agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat
    19561 gtcatgttga acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata
    19621 aatatccata agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa
    19681 aaggagtgtc tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg
    19741 tgaaattagc aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga
    19801 cgactgggca gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac
    19861 cgttaggcgg ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga
    19921 tttttcttcg agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc
    19981 gtttatctga tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac
    20041 taagaggact ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc
    20101 atccactttc attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat
    20161 gtagctataa aagctaaaca aactgataat atgcgatata atacgaacgt attggataat
    20221 ataaacaata atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct
    20281 gtgggaaaag atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga
    20341 tgggatgttg cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg
    20401 catgatacga tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac
    20461 gcacgtatga ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg
    20521 gtagcaatcg agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg
    20581 ggcattcttt ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga
    20641 atggccctct tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa
    20701 ggagcaatct acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg
    20761 gctacgctta gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg
    20821 atgtaccatg tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta
    20881 atatggttac cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa
    20941 aaaattttaa gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca
    21001 gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc
    21061 atctttttga agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac
    21121 taatatctgg tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt
    21181 tccttccatc attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga
    21241 gctggtagct tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt
    21301 caacagttgg cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa
    21361 tttttccggc aaacgttcta caaattcata catataagag gcttttaggg catcattgat
    21421 ttctgcgtca cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa
    21481 tagccaagta tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga
    21541 gcggatgtca tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag
    21601 caaattaaca agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc
    21661 tggataagca gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga
    21721 aacatgatca aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg
    21781 caaatcaggc ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa
    21841 agcggtttgc agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact
    21901 tgagtagatc gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc
    21961 caataggccg ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga
    22021 cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt
    22081 tagcagttca aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg
    22141 gttaccgatt cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc
    22201 gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga
    22261 acaaagaacg accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat
    22321 gatgattgcc actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt
    22381 gtccatatca tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg
    22441 aagtggcaat cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta
    22501 agctaaccga ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat
    22561 accaccgaat agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat
    22621 catttgatcg atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat
    22681 gattcctagt atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa
    22741 tcggcggagc gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat
    22801 gcgacgattt cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca
    22861 gcaatcttac cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt
    22921 tgggagataa taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag
    22981 tctgtttgat aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca
    23041 atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt
    23101 tccatgactt ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg
    23161 attttcagag cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt
    23221 tcacgaatag tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg
    23281 agatgatcca gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg
    23341 acatcataga aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca
    23401 atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca
    23461 gaatcaggag tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa
    23521 tcgggagtag tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc
    23581 acccgatttc ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc
    23641 agtgccaaaa gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag
    23701 ctaccaatat ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg
    23761 atcaaggttg tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc
    23821 gctagatcat ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg
    23881 cggatgacac gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt
    23941 tcttgcactt tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag
    24001 aaaataggca ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg
    24061 accactgacc cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa
    24121 gctagctgaa gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa
    24181 tgattcaact cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct
    24241 gtaccaaatc cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga
    24301 ccaatcacag acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc
    24361 atttttagtc cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca
    24421 gcttcaagaa atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa
    24481 cgtaataact gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa
    24541 gtactaccac attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa
    24601 tgaaagtttt attatgaa
//
LOCUS       DAZKOW010000034        24460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000034 DAZKOW010000000
VERSION     DAZKOW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.034"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(141..731)
                     /locus_tag="ACG912_001315"
     CDS             complement(141..731)
                     /locus_tag="ACG912_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HFQ0512647.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
     gene            complement(1049..2110)
                     /locus_tag="ACG912_001316"
     CDS             complement(1049..2110)
                     /locus_tag="ACG912_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0512648.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKITSFIKD
                     CIYYYRCNNNNSLTHNFRDNEFDCNIELVKKIEGDLSYILENEIFVNKTRRLLLGRCR
                     TCIQKAVRYASFNKEFDLNSYLSKILMNDELIACIHSYPYKQNPIKQRIFNYFLLNQS
                     IIGVKTLAYLSGMGRNRRF"
     gene            complement(2678..3745)
                     /locus_tag="ACG912_001317"
     CDS             complement(2678..3745)
                     /locus_tag="ACG912_001317"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0512649.1"
                     /translation="MQRVLMIGNDSSVKGGITSIITQMLNFNWEKKNIEMTFIPSYKG
                     GNAIFKSLYFMYAFAKIIGISLLSKPNIIHMHMSYRGSFSRKYVLNKWFRLLNIPVII
                     HLHGSEFKKWYDSVSVKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENS
                     IVVPEYTIKWNSKMCRFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDE
                     EISLKELIEEYNLQSYFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYG
                     MPVIATDVGDISSAVRDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAIT
                     KFNDGNFFDRILNLYQKGSKE"
     gene            complement(3765..4244)
                     /gene="pssE"
                     /locus_tag="ACG912_001318"
     CDS             complement(3765..4244)
                     /gene="pssE"
                     /locus_tag="ACG912_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssE/Cps14G family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0512650.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNTETIINSIEVLIEKI"
     gene            complement(4261..4722)
                     /gene="pssD"
                     /locus_tag="ACG912_001319"
     CDS             complement(4261..4722)
                     /gene="pssD"
                     /locus_tag="ACG912_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PssD/Cps14F family polysaccharide biosynthesis
                     glycosyltransferase"
                     /protein_id="HFQ0512651.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(4736..5986)
                     /locus_tag="ACG912_001320"
     CDS             complement(4736..5986)
                     /locus_tag="ACG912_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFQ0512652.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
     gene            complement(6364..7815)
                     /locus_tag="ACG912_001321"
     CDS             complement(6364..7815)
                     /locus_tag="ACG912_001321"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFQ0512653.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(8136..8900)
                     /locus_tag="ACG912_001322"
     CDS             complement(8136..8900)
                     /locus_tag="ACG912_001322"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFQ0512654.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(8928..9626)
                     /locus_tag="ACG912_001323"
     CDS             complement(8928..9626)
                     /locus_tag="ACG912_001323"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFQ0512655.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(9638..10417)
                     /locus_tag="ACG912_001324"
     CDS             complement(9638..10417)
                     /locus_tag="ACG912_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFQ0512656.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(10433..11377)
                     /locus_tag="ACG912_001325"
     CDS             complement(10433..11377)
                     /locus_tag="ACG912_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0512657.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(11423..12202)
                     /locus_tag="ACG912_001326"
     CDS             complement(11423..12202)
                     /locus_tag="ACG912_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512658.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(12582..14657)
                     /gene="glyS"
                     /locus_tag="ACG912_001327"
     CDS             complement(12582..14657)
                     /gene="glyS"
                     /locus_tag="ACG912_001327"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFQ0512659.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(14659..15576)
                     /gene="glyQ"
                     /locus_tag="ACG912_001328"
     CDS             complement(14659..15576)
                     /gene="glyQ"
                     /locus_tag="ACG912_001328"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFQ0512660.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(15962..16780)
                     /gene="recO"
                     /locus_tag="ACG912_001329"
     CDS             complement(15962..16780)
                     /gene="recO"
                     /locus_tag="ACG912_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFQ0512661.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(16923..17822)
                     /gene="era"
                     /locus_tag="ACG912_001330"
     CDS             complement(16923..17822)
                     /gene="era"
                     /locus_tag="ACG912_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFQ0512662.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(17837..18232)
                     /locus_tag="ACG912_001331"
     CDS             complement(17837..18232)
                     /locus_tag="ACG912_001331"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0512663.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(18216..18692)
                     /gene="ybeY"
                     /locus_tag="ACG912_001332"
     CDS             complement(18216..18692)
                     /gene="ybeY"
                     /locus_tag="ACG912_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFQ0512664.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(18721..20913)
                     /locus_tag="ACG912_001333"
     CDS             complement(18721..20913)
                     /locus_tag="ACG912_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFQ0512665.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(20937..21908)
                     /locus_tag="ACG912_001334"
     CDS             complement(20937..21908)
                     /locus_tag="ACG912_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFQ0512666.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            22613..23992
                     /gene="brnQ"
                     /locus_tag="ACG912_001335"
     CDS             22613..23992
                     /gene="brnQ"
                     /locus_tag="ACG912_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0512667.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            24274..>24460
                     /locus_tag="ACG912_001336"
     CDS             24274..>24460
                     /locus_tag="ACG912_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0512668.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIK"
BASE COUNT         6985 a         5096 c         3973 g         8406 t
ORIGIN      
        1 taaaattgct cttggggcgt taaaagtata tacaagaact atggagattg caaaaaatgc
       61 caacgtgaac aatgtccaat ctgctcctga ccatcttttt attgaaatac gcatcgttat
      121 tcaccttctt tcgctaaaaa ttattctaaa aatacttttg ttaaattaac ataccctatt
      181 atttttgccg gtacacctgc aagaattgca ccattctcta gacaatcttt gataacaata
      241 gctcctgccg caacaactac attgttagct atttttacat taccaataat ctttgcaccc
      301 acgcatacac gtacattatt tccaattaca ggacacttag gatcgattcc atcatttcca
      361 atacagttat tcccatataa gtaacaattt tttcctatta tggcgttacc attaataaca
      421 attccttgtg tatggtaaaa tcttacacct tcatcaaata cattttcacc acactctata
      481 ccacattttc ttcccaaact attcattctt ctgcaagaca caatatacat aatcgcataa
      541 atgatatttt tctttctctt tgaataataa taagatgtaa ttctcattcg acgagtatac
      601 cgccaagcgt tgtagtccgg atgtgatttc attattttta gtaattttgt gtgttgtcct
      661 ttagattcta tatatagatt cttttccata cgaagaaccg cttttaaata ttctttactg
      721 ctaatttcca ttcatatctc ctattaattc aatttatact taacaatttt attcggattt
      781 cttgcaacaa gagcatcatt caatgctact gaatctacaa cgatggatat gttatcttca
      841 tcgttgattc caccggatac aatattactt ataaaaatcg taacattttt tccaaaagaa
      901 gggatgtccg tgatgtataa tgcccatttt ccgatatttc cagttattta aacctaacga
      961 atttttacaa tagtacaagt atcacaatca ttaatatata actggaatat aaatctccaa
     1021 aaattcacta tagtacagtt ttttatgatt aaaatctcct atttctaccc attccgctta
     1081 aatatgctaa tgttttaacc ccaataatgg attggtttag taaaaaatag ttaaatattc
     1141 gttgtttaat tggattttgt ttatatgggt atgagtgaat acaggcaatc aactcatcgt
     1201 tcataagtat cttagacaaa taggagttta aatcaaattc tttattaaaa ctagcatatc
     1261 taactgcctt ttgaatacaa gtacgacagc gtcccaacaa aagtctcctt gttttgttta
     1321 caaatatctc attttctaat atatagctta aatcgccttc aattttttta actaattcaa
     1381 tattacaatc gaactcatta tctctaaaat tatgtgtcaa tgaattatta ttattacatc
     1441 tataataata gatacaatct ttaataaaac ttgttatttt tgaatgttgc aaatatgaaa
     1501 tatggaatat gatatcttca gatatataat ccctttctga cggaaacttg atattgtgct
     1561 gttgaattat ctcagatgaa tacagcgcat gccaaactga cattggcaat ataaaatcga
     1621 ctttataact cggctcagtg ccaatcattc caagcaacac tttttcaatt atagcgttat
     1681 ttgtaaatat cgtactatca tatcttgctg gctttgaaat aatattatta tcatcataat
     1741 attcataata tccagcataa catgtgtctg cattactcct ttttatacca tttaacattg
     1801 cttcaatata attagctgca acaaaatcat cagaatcaat aaaagcaaca tattttcctt
     1861 ttacatgttc aagtcctgta tttcttgcca atcccaagcc agcattattt ttatgaataa
     1921 tacgaatcct tgaatccttt aatgcatagt cttcacatat tttattagat gaatccttag
     1981 atccatcatc cacaattata atatctaggt tcttatatgt ttgattacac actgattcca
     2041 aacaacttcc tacatacttt tcaacattat atacaggtat gattacacta attagatcac
     2101 tcgaattcat ccttctatcc tttcactcac gttttctcaa ttagtcaatg acggcatcaa
     2161 cgattttctt aaattctctg aaaaaaatag actctgatct tgatgatgtg aatgacatac
     2221 tatcttcttc tagaaaaatt atttccttta aattatttat taaatcaata atatgatcct
     2281 cgtaagtatg ctttcccttg gaataatgaa aatatacttt tgtattacac aatattgcaa
     2341 ttttatttct taatatgatt atttcactca ttcattactg ccacatatac acaacttata
     2401 accaccccct gcaaagttta gggtaagtta tcagataata tcttcctctt catcatccag
     2461 aatacaaaat ctatttattt atagaaatat cattaatgtt tttatataat gtaccgtgct
     2521 tgtactcttg ctctattaca agcactaaaa cactactatt catttgaatt cttacacgga
     2581 taaaaattta catcgtctat ttcaatattt tttctctcta ttttattcac ttaataagtt
     2641 cattacataa tcgaaacatt ttttattttc cttgtcatca ctccttgctc cctttttgat
     2701 acagatttaa aattctatca aagaaatttc catcgttaaa tttcgttatt gccaaatcct
     2761 tcgaacaacg tgaaaatttt acgtatttat ctttatcatt gatttttagt attgcagatg
     2821 tcaacgctga tacgtcacca ggttgaaaca aatatccatt ttctccatca cgtactgctg
     2881 aagaaatatc tcctacatct gtagcaatta caggcattcc ataagaaatc gcctctagaa
     2941 tagatatagg caatccctcg ttataggacg gtaaaatcat cacatttgat cttctatagc
     3001 actcctcctt tttttctccc acaacccaac ctgtaaattc aaaatacgat tgcaaattat
     3061 attcttctat caattctttg agcgatattt cttcatcacc tgctccagca atctcaaaaa
     3121 taaacttttt ttctggatat tgttctgcaa tgtttttaac tgcttctacc aaatcgtgta
     3181 caccttttcg ctttatcaaa actcccataa acagaaatcg gcacattttt gaattccact
     3241 ttatggtata ttctggaact acaatagaat tttctacaac aacagtttta caatttggct
     3301 caatttcttt aattatcttc ttccattttt ctccaagtac aatcgtggaa tcagactctc
     3361 ttaacagcaa gcgaatctga ttttgttttt ttacagaaac actatcatac cattttttaa
     3421 attccgatcc atgaagatgg atgattacag gaatatttaa taatctaaac cacttattca
     3481 gcacatattt cctactaaaa ctccctctgt acgacatgtg catatgaata atattaggtt
     3541 tactcaataa agagatacct ataattttag caaatgcata cataaaatat aaagatttaa
     3601 atattgcatt tccaccctta taactcggta taaaagtcat ctcgatattc tttttttccc
     3661 aattaaaatt gagcatttgt gtaattatac ttgtaattcc accttttacc gagctgtcat
     3721 taccaatcat aagtactctt tgcataacgc tttactcctt atatttatat tttttctatt
     3781 aatacctcta tcgaattaat aatcgtttcc gtgttagata catacttgtt aaacatccga
     3841 actcttgttt ctttaacggc atcttctaaa ttttctaatt cataacaagg atagataata
     3901 ttcatctcat caaattgtct aattaactgc agttggtgat catcgacatg ctcaccatac
     3961 ttagccaatc gaggaacagc aatgaccctt ttaccttttt ttacggcccc aattatagca
     4021 cctgtacctc catgagtaat caccatttct gctttttgca tagtttcagt aaactcatcc
     4081 ctatttagaa atgctttata ggaataattt agtggtttat aatcactata accactctgt
     4141 gcaaatgtac tttcgattat tactcctttt tcaattaaat catcaatagc ttttaacaat
     4201 ctatcaaatt gaaatttttg tgaacctaat gttataaata tcattttttt ctcctattat
     4261 ttaatatatc ccacccaaat aaatagcatt aggataaaat tgtagcatag tttcccactg
     4321 aacataaaat tgatctgcaa aatgatacat caattttcct gtctgagtag ggctggttac
     4381 ttttgcaaac gattcaatat aaatcagttt ttttcccatc atttttgtta ttaagcagat
     4441 agggatcata gctaaaacac ctgtacaaat cacgagatcc ggtttttcct ttatatatat
     4501 aataattgat ttgaataaat taataagtat agtacacaaa aaaaactttt cttttctatt
     4561 tacttgtctc atgtagtacg ttttttcgcc ttttgactca gctgaatatt ctgtctgttc
     4621 tgttataaca aaactctcat acttctccat cagtggtttt aacatcatta actgttcata
     4681 atgcccccca gaggaagccg caaaacatat ctttttgttc atattaacct ctctatcagt
     4741 cacgctgaaa aatatctcta gtgtaaactt tttctttaat atcatccaag caggtatcat
     4801 atcgattggc aataatagaa tcacacatcg ccttaaactg ctctaaatcg ttgataacct
     4861 tacttccgaa aaaagtctct ccatctttca gtgcaggctc ataaatcacc acagtcgcac
     4921 ctttagcttt tacgcgcttc ataactccct ggattgaact ctgacgaaaa ttatcactgt
     4981 tgctcttcat ggtcagccgg tatataccga ttaaaactgg attttccctg ttgacatcat
     5041 actgattata ttcagcgtag ttgtaatagc ccgccatctg aagaacacga tctgcgatga
     5101 aatccttacg ggtacgatta gattcaacga tagcctcaat caaatttcca ggaacatctg
     5161 catagtttgc caacaactgc ttggtatctt ttggcaagca ataacctcca tatccaaagg
     5221 atggattgtt gtagtgagtt ccaatacgag gatcgagaca aacaccattg attatctgct
     5281 gagtattcag ccccttcatt tcagcataag tgtctaattc attgaaatag ctgactcgca
     5341 gtgctaaata ggtatttgca aacagcttta ctgcttcggc ttcagtgaat cccataaaaa
     5401 gagtatccac gttctcctta attgcacttt tctgcagaag tgttgcaaaa acatgagcag
     5461 catttaacaa gcgctcatca gtcatatcag taccaacaat aatacgagat ggatacagat
     5521 tatcatataa agccttgctc tcgcgcagaa actctggact aaaaataatg ttcttactcc
     5581 ccgttgtctc acggactgat tcagtgtagc caacaggaat tgtagacttg atgaccatga
     5641 tggtatcagg attcacgtcc atcacaacct caataactga ctcaacagca gaagtatcga
     5701 aatgctgagt acgactgtca tagttagtcg gtgtagcaat aacaacgaaa tctgcggatt
     5761 tataagcgct ccttgcatcc aaagtagcag ttaagtcaag ttctttttct gtaagatact
     5821 tttcaatata ttcatcctga attggagact tccgtttatt aatcagttca atcttttcag
     5881 ggataatgtc taccgcggtt acctggtgat gctgcgccaa tagtgtggcg atactcaaac
     5941 caacataacc ggtgccggct actgcgatgt tgtacttttt cttcacaata tctcctcttt
     6001 cctctcttaa gtatggagaa tgttactcct acggctattc atcatgtcaa aataacacag
     6061 cttagaatgt tgataaaata attttccaga atgtgataaa atctctttca tttataaacc
     6121 acacatctaa ttttatctat tctccacaag acatgtagag tattctttaa taacaagata
     6181 cgaccttact aaatacttca aaaaattatc atatttaatt cataaatata aatcttcatt
     6241 ttaaatgtat gcccaaaaat ttatctgtaa tgcgtcatca aaatctagat tccaaatctt
     6301 tatcaatttt tatccactca ttctaccact ttagtaatat tttctattta taacactttc
     6361 gtcctacatt gatccccccc tctgaagtac agtttttact gtcttaaata aaattttaaa
     6421 atctaacccc atactccaat tttttatata ttccgtgtca agttttgcta cttcttcaaa
     6481 atcagtaata tcacttctac cacttacctg ccacattcca gtaattcctg gcttaatagc
     6541 aattcttgca cgatgatgat aatcatattc ttcatactct gatttaaggc atggtcgaaa
     6601 tcctataagg ctcatctttc ctgttagaac gttgaaaaat tgagggaatt catctaaaga
     6661 cgtggatcta ataaattgac cgattcctgt cttttttgtt ccatctggaa gtattttatt
     6721 tccgataatt cttggatcaa actccatctt aaacataaga ttattgctca tcttattttc
     6781 tttcattaat tctacttttc tttcttctgc atccatatac atacttctaa acttatacat
     6841 cttgaacggt ttaccattct gacctatacg ttcttgtgca aagaaaattg ggcctggtga
     6901 attaatataa ataagtggag caataaagat aaaaataatt cccgttgcaa tacatccaac
     6961 aatacctgca agaatatcaa ttgttcgttt tataaatgcc tgttgagttg tcataacatt
     7021 gatacttgtt gtaagtactg tatattctcc tatacgacca atagattgtt ttcccaaagc
     7081 actatctgat acttttgcaa gattcaaatg tacagtaagg cccatttcag agcatcgttc
     7141 aataaattct ttcatacacg aagtactttt attcaaaata ataaagactt catctaccca
     7201 tccctgacat aagtaatccg ctgcattttt ggaatcagaa acaactgtca ctccctcaac
     7261 atcttcacca atcatgcttt tatctatgat aatgacacca tttattttat acattttata
     7321 gttatgctct ttcacattac gaataactac ttcagcaata ctactagatg taactataat
     7381 aagtgatttg ttcccctcat ttttcatttt atgtttcaag taatgtttcc ataagaggcg
     7441 agttatatat gtaattacaa cgtataaacc tcccatcaag aaaagaacga ctctagaata
     7501 atcatttcca ccctgaacac taatgagata caaacttcct aacagcacca acataatcgc
     7561 ttgctttaca gaagaagcaa attctatata gtaccctctt tttaatacac ctctatatgc
     7621 ttcaaacatg aaaataacta aaagatctgc cagttcaatg aagactgcca tgttttgata
     7681 cattggaatc acataaggat tccaatctac ctggcgtaag acatacgata gataaaaagc
     7741 aagctggata cacaataaat ctaaaataat aaaatcaata tgtttaaccc atccccttga
     7801 acttttcttg tacattcctt tttactcccc tctttttaat aaaataaatt gtattacttg
     7861 ttttctctcc taaatatctt tctgaaggat tattgactcg acaaattggc ttgtggatat
     7921 tcttatatat agaacatccg taagccaact taaaactcct taacaaaatt tttttactca
     7981 tattgtagat caaaacaagt atccaagaaa tcatatttta tttctttttt ctgcttactc
     8041 aacaacagct aaaagaaaaa tcatcataaa caaattcaac taagccaacg atccccttgt
     8101 gaaatcaagt tttttcttga tttacgaata aagagctaaa ataaaccaaa cttcttcttt
     8161 tttacctctt catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact
     8221 tgtttcatct gtactacttt ttccttacca aagtcctttg caatctgctc atagcactct
     8281 tttaagtaaa aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga
     8341 ttatgtttga ccatcgtttt ggctgttttc tggatgtctt tgccaaatac cccaacataa
     8401 cttggcgcag tgagttgggc caaacatccc atatctagga acggaagcaa atgatttggg
     8461 tcttcgcgaa aatgcacgtt gcgttctgga tggacgatta ctggtgtctt tcctagttct
     8521 aacaactcaa aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc
     8581 agaagatatg tatcatctaa atctgtaaat aatatttcat ctcggtggat gtcttcgatg
     8641 agcgttccgg taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ctgtcttttc
     8701 tctaattcgg cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat
     8761 tttccattat tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt
     8821 tcagccattg caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata
     8881 tggcaatgta gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag
     8941 taataaccag catctttgct atgttctgct ccgttgtaca caacacctaa gactcgtgct
     9001 tgtaccatct ccagcagttc ttttgcttta gtcaaggatt ctttacgtga catattttca
     9061 cgtacgacca ataaagtgcc atccgttttt aaagacatga tctgtgcatc tgttacggct
     9121 actactggtg gcatatcgaa tatcaccacg tcaaataact gtcttgcttc ggataggatc
     9181 tgatccattc ttggcgagct taacaattct gatgggttgg gtggcttagg tccactaggc
     9241 aaaatcgaca agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa
     9301 ctaagtgctg tacttaatcc actggcattg ttcagctgga atgtcttata gacgacgggt
     9361 ttacgtaaat ctgcatcgac tagtagaact cgttgacctg atttagcaaa aacgactgca
     9421 atgtttgctg cagtggtgga tttcccttct ccaggacctg atgaagtcac gacaatcgtt
     9481 ttgatttgct gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt
     9541 tcggcgattg gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct
     9601 gctttttgtt tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc
     9661 gtgatggggc tgttttatcg tttgtagctt ttggtcgtgc aggagctgtt ccttttttac
     9721 ggataccagc gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc
     9781 ctaatgtatc tgtcacatat ttttcatctt tcactgtccg atcgagtaat tccaataaga
     9841 atgccagacc tacacctacc atcattccta acattaagcc agtcattaaa ttcaatttat
     9901 tatttggcga aacaggtgtc gtattggcaa ctgcacctga aatgatcgag attttatcaa
     9961 cattcatcac atcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt
    10021 gagcagccac tgcattcgta cttattgcct gaatcgagaa catttgtgaa ttttctgcct
    10081 gattaactga gatggcatct ttgatttgtt cagcagtcat tttcacattg tcttctgttt
    10141 ctaaacgatc tttgacttca ttgagcacta aatcactgac gatcatatct ttatacgtat
    10201 tgatcatttg caggttcgta ttgacatcat tgacatttgc agtgtctgat tgtggcagtg
    10261 tcacgatcaa ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc
    10321 ctgagatggc caagccagca aacattgaga tgatgattat taccaagtgt ttgcgtaatg
    10381 tttcaaaaat ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat
    10441 tctctagatt catttgatct tttaattctt ttttcacacg atcaagttct tgatcactga
    10501 ctttttgata ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttaa
    10561 tcgtaccaaa tgcctcccga taatctaaag caattttttt catttcatca aagttcatgt
    10621 ctgttttcat attcgactct agagcattca aaacggattg atagttgctg acaccgttta
    10681 gactcaatac ttttttcgct acgccttcta cgatctttct ttggcgccct tgtctaccgt
    10741 aatcaccatt tgggtcttca taacgcatac gagaataaga taatgcctga tccccgttca
    10801 atgtaatttt accgattgga aagtcatagc catcttgtga gaaagtgagg tcattattga
    10861 cttcgatacc accaactgca tcaacgagtt cctttagtcc tttcatattg atagagacat
    10921 agtgatcgat tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc
    10981 cgaatgcgta agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg
    11041 tatctcgagg aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca
    11101 tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc tccagtatca atccccatca
    11161 atagaacaga gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg
    11221 ttcgttccac tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg
    11281 ctcctactcc tacaattgct aatactaata cgataagaac tgctaaaata atcaaaaata
    11341 tttttttccc tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt
    11401 gcttgaatca acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa
    11461 cagtttgtgc ttttgttgcg tatgcggctc ttattttttc attccggatc tgctctattt
    11521 gactgagcgc cgtcaaatat tgatcgtaag tagcggtagc tgttacttga ccgtctttca
    11581 gcatcgtatc gaatagctga tctagttgcg tgacttgtgc aatttgggta tcagcgaatc
    11641 ctagatattg cataacgact gttttccaag cactatcctt aaaaaagctc atattttcac
    11701 gtacatgggc gacatctgca cttgccaact tttctttgat gatgacatca tccacagctt
    11761 gctgccagtt tgatacgttt ttagtaaata atttattgac agccgtctgg atcttcagtt
    11821 ttgattctgc atcctctaac tgcgttaaga ctgctttctt ttcttcttgg atcgcagcag
    11881 cttctttagg taattctgat tccttgatgt cataatcatc tgccgaaagt ttgatagaag
    11941 atagtttatg tgacaaattt gaaactgttt cttctgttat ttcaggattt aggaaaattt
    12001 tttcttttgt actgtacagc ttatctactt cttttgataa agaggtcacg gtgtcttttt
    12061 gattggtgat ggcagattct gcgtaattga tccgttgatg atattgccgc tgattattga
    12121 gatggtatcc aaacaagcaa atcacaatag cgcctgtgcc taccgccaca gtagccaata
    12181 acttgacgaa cggcttttcc atagattcac tcctaaatat tgatgataga taagtaaaga
    12241 tgtgattcat tcacatcaga atgagcgctt tttattttaa aagcataatt aaactaatat
    12301 attcatagaa atatttataa acaggttttc cctatataat cataccacat acagtttttt
    12361 tgactaggga aattcctaat ttaaaagtaa ataaaacagt aacaagatta taattaatcg
    12421 ttttcatttt tatttcctca cttttcatga gcgttttgaa aattcgaaca aagaataaaa
    12481 taacaaaaat tattaaaata gaattaataa cagctattta taacaaaaaa aacagagaca
    12541 cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt gatgatttga
    12601 tccaagcttg ctaatgacaa agccatggca gctatttgac ttagttgtgt tagtcggttc
    12661 ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt ttcgatcagt
    12721 ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc aaaacttgct
    12781 ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa cagctcaggg
    12841 tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac acgtgtcaaa
    12901 gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa ttgtgctgct
    12961 gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat gacatcatga
    13021 cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc gatcacttgt
    13081 tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat aacatcgcta
    13141 atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat gatccgaacg
    13201 acaccgtatg cttggcgacg taaggcataa ggatcgtttg acccgcttgg aatcatccca
    13261 actgcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac agaacctact
    13321 gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg aatcgctgtc
    13381 gctacagcag gtttctcgcc tttcagaaga gcatattttt cgcccatgat cccttgtagt
    13441 tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc agcagctcgt
    13501 tgcaaatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat acgggcaatg
    13561 acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa agtaacagtt
    13621 tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata aaagaattcc
    13681 gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatc ttccaatttt
    13741 acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg atcattccgt
    13801 acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc aggaacagaa
    13861 agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa attgttgact
    13921 tcttctagta aatcttcgtc tagatcaata acccagtgat ttttttctgc taattcgttt
    13981 gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa ttgttcattc
    14041 aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa gaaacgatga
    14101 cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac ttcgtcatct
    14161 aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc tgcccaatgc
    14221 atcgttacag ggaaagtcaa acttttgatc acccctgcta agcctgtcaa tacttctttt
    14281 gcttccttac catgagtgta tttcgttaca tagacatatt ccacaccttt taattctcta
    14341 aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc tgctttcgtc
    14401 cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc ttcttcactg
    14461 tccgcttgtc gttctggaat atttgttact ttgattgcta aacggcgcgg tgtggaaaat
    14521 gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt tttttcttct
    14581 aattgtttca tgcttggtgt gacaacatgg gcaggcattt cttccagccc gatttcaagt
    14641 aataagtctt ttgccatctt attcagcctc cttcacggtt tctggatttg ctgtgttttt
    14701 attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg ctacagcacg
    14761 agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata ctgcaccgcg
    14821 ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg ccggatgaac
    14881 aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt tgcttagtag
    14941 caagtcttgg tcgcttactt caaaagaata tttggaatgt tcatattctg gttgtttgaa
    15001 gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt caacttcttg
    15061 gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg gtttacaagg
    15121 cagtccacca acttgctgga agtacgtgaa ttgtgtgatt tccattccgt caagccatac
    15181 ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt caacaaaacg
    15241 gatgtcgtgt tctaatggat cgattcctaa aaggcgcaag ctttctaaat aaagctcttg
    15301 aatattctca ggtgaaggtt tcatcaccac ttggaattga tgatgctgat acaaacggtt
    15361 tgggttttca ccataacgtc catcagctgg tcggcgagat ggctctacgt aagcagcatt
    15421 ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac ctgctccttt
    15481 ttctgtgtca taagcttgca tcagcatgca gccgtttgat gaccaaaatt tttgtaatgt
    15541 taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt tcaattttaa
    15601 agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct atgtctgatt
    15661 acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag attcggcagc
    15721 ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc tcccactctc
    15781 ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta ttcattttgc
    15841 agttttcttg tttctataat agatcctatc actgaacgat ctatttaatc tatggttaat
    15901 aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta tgttttattt
    15961 actattcttc tttttcaggt gtctcttttt ctttttcaga catgtctttc tctgacgtct
    16021 ttaatacgtg tgcccaagac ttcatttgat tgatgaactt tttgcttttc aaatggatac
    16081 cgacatattc atcatacagc tggtcgatga gttcacggat tgcttgtttg gtttcttctt
    16141 tcagatggat tccactaatt ttatcataag aaatagctga aaacatccga acgaaataaa
    16201 cagctcgagg atctgcatga taacggtgat cgtccatcga ccaatgtctt tcgcatatca
    16261 caccaccata tttggaagaa tagtcaaatt ttccttttgt ttccccgcat acgacgcagt
    16321 gtgtccagat tggtgcaatt ccgaagcgtt gcagtatttg gacttcaaaa atattcgtga
    16381 tgatttctgc atcgtctcct tggtccatcc gttgcagtgc ttgttgcgta aactggaaca
    16441 aatgcgggtc gtactgatga tcttcgattg ccgcatctac tagattcaat aaatatgtgc
    16501 catagccgct caaaaagatg tcttcttgga tgtgtcgaaa cgcatggaca tctttgacac
    16561 tgcttaaaaa tgacaaacct tcacttcgaa aattcccgat ataaactgct tcagtaaaag
    16621 ggagcaaagc cggagctaag ggctgatttt gccgatggat ctctttgata taaaacatca
    16681 ttttgccaga agattctgta aagattttga ctaatttatc tttttctttg taattacggc
    16741 tagataaaat caagcctttt gattctgcaa gctggctcat attctccctc ctaaaaaata
    16801 aatctggaaa agcagattct gccgatccag atcatttatg ttgcgatctt ccgcagcttt
    16861 tctcaacacg cggattttta ttcagtttat ttctatacct aatttacaag aagctcagaa
    16921 tatcagtatt cgtcttttcg gtagccgaag tcttgcagat agactttttt gtctcgccag
    16981 tctttttgaa ctttgaccca aagttctaga aagactttgt ctcctaaaag attttcgata
    17041 tcttttcttg ctttcgttcc tacttgcttc agcatttttc cgcctttgcc gataatgatc
    17101 cctttttggc tatctctttc gacaataatc gtagcctgga tatggatttt atcattctcg
    17161 tttcgcttca tcgaatcaac aacgactgcg acagaatgag gcacttcgtc tcgtgttaaa
    17221 aacaagactt tctcacggat caattcagaa acgatgaagt attcaggatg atcagtcact
    17281 tgatcatctg gaaaatattg tgggccctct ggcatttcac tgactaatac atccatcaaa
    17341 cgttcgacgt tgtttccttc tgtagcagag attggaatca cttgtgcaaa aggcatctgc
    17401 gttgaataat cttcgatgat tcccaacaac tcgtctggat ggattttgtc gattttattg
    17461 ataatcagat aaacaggaga atgcacgttt tttagacgtt caataataaa atcgtctccc
    17521 cgaccgcgtt tttgatcagc actaatcata aataagaccg cgtccacttc tttcaacgca
    17581 ctgtaagctg cttctaccat aaaatctcct aaacgatgct ttggtttatg gattcctggt
    17641 gtatcaataa aaatcaactg agcttctggt gtagtgtata ctccttggat tttatttcta
    17701 gttgtctgtg ccttgtcact catgatggca attttttgac ccacgattcg attcaataat
    17761 gtggatttcc ctacatttgg gcggccgacg attgcgacaa accctgattt atgttctggc
    17821 atatatgttc tcctttttaa ttaaacaaca aatgatatat tttagggaca aataaaatca
    17881 acccaacgat gacggtaaaa caagcggtca ctaaaactgc tccagctgcc atatctttga
    17941 tttttttccc aatcggatga aaatgaaaat ccgtaaacat gtccacgaca ttttcaaaaa
    18001 cagtattgat gatctctact atccatacga gaaaaacagc taaaagcagc cataaccatt
    18061 ctatccgatc aagttggaaa atcacgccca taaacaaagc gaggattccg aacgcaacat
    18121 gtttcctcat gttgcgttct tcctcaaaaa cagttttgac tccttggacg gcaaattcca
    18181 cggacatcag gaaatgtttg tttttctcta cgtttttatc ttttaagtcc ataggcatct
    18241 aagatctctt tctgtaaacc aaacatttct ttctcatcct ctggtgtcat atggtcatac
    18301 ccgttgatat gcaagaatcc atgaaccgct aaaaagccaa gttcccgatc atacgaatgg
    18361 ccgtaagctt ctgcttgctc tgcagcacgt tcagtagaga tcatgatgtc tcccagtaca
    18421 cgaggcaatg gattctctcc atcttcaaaa atcactggga tttcgtcttc gccctcttct
    18481 tccaaggcaa aactgatgac gtctgtcggt gcatcttttc cacggtaatc acgattgatt
    18541 acttggattg ctgcattgtc catgaaagta acagacattt ctgtttcttc tggcaactct
    18601 aaatatgatg ctgcaaattg cagcaagtcg tcgatttctt tcattttctc ttctgagact
    18661 tggtttgttt catccatgaa ggtaatatcc attatacatc gcttctttct attcttcttg
    18721 ctattcttct tgttcttctt ttatcttctc aacttccgat ttcattttag gatatttgat
    18781 ccgagaatgg aaggtactgc ttaaaccgct gatcaatgat ttttctactt tacggatttc
    18841 ttttaacgtc aatccactgt cgtccaattg tccatcggaa atacgctctt caataagatt
    18901 atggacaaaa ctttcgattt tttcgctcgt tggatggtcc attgcccgta ctgccgcttc
    18961 acaactgtca gctatattga cgattcctgc ttcacggctt tgaggttttg gtccaggata
    19021 acggaattgc tcttccgtaa cttccgggtt gcgttccttt gccttaacgt agaaataacg
    19081 catcagcgtg gttccatgat gctgtctaca aatatcgatc accatttgcg gcatattata
    19141 atcttccaat attttggcac catctgtcac atgtccaaag ataatatgtt tactgtcttc
    19201 tggcaataag aaattgtgcg gattttccgc tcctgaaggc aagttctcaa cgaaaaagct
    19261 ggcgtgtttg atcttgccga tatcatgata atagcaagct actcgagtta gtaaagaacg
    19321 tccaccgatt tctgctacag cattagcact tagattggct accatcatgc tatggtgata
    19381 cgtacctggt gcttcttcca gcaactgttt caataaagga tgattgggat tgctgagttc
    19441 atttaatacg atcacactat catcattgac catcaattca atataggggt gcagacctat
    19501 cgtcagcaag aatgaaaaca gtgcacctgt gaatccgcag actagcgtta accaagtcgt
    19561 tgcatcccca aagctcattc cttggtagat aatcaaaatg acatccatca tcacaggaaa
    19621 aataatgacc cacatagcag cggataaccc ttgttctgag atccgtttgc gtctgacgat
    19681 cgttgccatg agaccagaaa atacatagga catcaaaata actgtcagaa agttcgtgcc
    19741 aatcgaatca taaaaaatga aaagtgcgga tactgcttga aaaataccag ccatgatccc
    19801 tgctcttcga ttcaagaaaa agcttaaaac aagtggagca aaagccgcgg ggtaaaacag
    19861 cggaatataa gcagcttgtt ctgtttggaa gagttggaaa aatttcatta gtacgacgct
    19921 tactgacatt gccgtcacgt agaaaagtac gtattttgtt ctttctcctg cattttggaa
    19981 ttgaagtgaa ttataaatca gtacaccgat ttgaagcaaa acagctaaaa ccatggcaat
    20041 gagtgggaaa atagaagtcg tttgattcgt caatcctagt aattccaatt ttttcatcgc
    20101 tgaagcatca atttgatttc cttcgcgaac aataatttct ccttggaaga tcatcactgg
    20161 ttgtacagct tctcgtgcag attgttttaa ttcttctgtc tttttctcat tcaatacatc
    20221 attaactacg atgccttgat ccactaagta gcgaatcatt tgttgctgct ctgttgtgac
    20281 attcaaatat tgaatctgat cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg
    20341 aacttgttta ctcatctgtt cgtcaatgag cttcaaactt tcatccctaa ctgtgtgaat
    20401 ttccgtttca gtcatttcaa atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa
    20461 cgcaacgttt tctgcattca cgttttcaaa atttttcttc agtgtagcaa tccgctcttc
    20521 tgtagtaggt ttcgggatat tttcatcttc cttcgcctta tctaccttgc cattataaga
    20581 tttgttgatg gtatcattcg tcttatcgat caattcgaat aattgcttga tccgattgtt
    20641 ctggtcatct gccagatctt tttgataagt atattctgga gtcactgctt ctgctgccag
    20701 ttttcttctt tgttcggttt cttccgtgtt ctcaatcgtt ttattcgcac ggatactctc
    20761 ttctgctact tggccttctc tgaaatcaat ggccttttgt ttgacactac taaatgtaac
    20821 gccgcagaca atgataaaaa acaagaacag aaggaaaggc aatgctgcct ttccgagttt
    20881 ttcttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
    20941 tcttgatgaa gatccgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
    21001 acaacgtcac ttgcttcaaa attaacaaaa tcgatttttt tgatttgctt taatgttcgt
    21061 tctgcatgaa caagaccact tgtcgtccca cgaggcaagt ctatctgact tgtatcccca
    21121 ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat ctgagcgact
    21181 gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgtccg cccccgcatg
    21241 taagctaaag gtgcgatttc gatcacgcct cgttccatca atcggttcgt atgatccaat
    21301 ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac tttttctttc
    21361 agatccccgg gtagaaagcc taggttttcc cctgcctcca cagcgggccg cgtcaaaatg
    21421 attttttgca cttcgccttt ttttagggca gcaattgcta atactacggc aagaaacgtt
    21481 ttccctgttc cggctggccc tacaccaaat acgatgtcat gtttggctac cgattcaacg
    21541 tatttctttt gaccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
    21601 tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat tttcaatgcc
    21661 gtcacaacat ctggcgtact tacgtggatt cctcgtttga tcaattcctg taatgtccga
    21721 atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat gatttctcca
    21781 cgtgtatgga tggttgtttc cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca
    21841 cctaaaagca tgctgacatc atcagcagct gtcaatctaa tctctaaaga actagactct
    21901 tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt accatactca
    21961 gaagaaacta tatagcaaat aagaaaaggt tgtaaaagag tcgcttaacc atacgtacca
    22021 agaaacgtta aaaaatagcc gtttccattc gctaaaggtc tcgttttgat aaaaagaagc
    22081 tgagaatgta agttatatga aagtggagaa caaaatattt cgtttcattc aacaagtatt
    22141 ctctaggtat ttttcctgtt tcaaaaaaag aaaacaaaat agttaaaata ttcttgcatt
    22201 tcaaaaaaaa caggtgtatt attacgtcaa caaataaaca tacagcaaag cgtcgaggaa
    22261 aacagtaggc tattcaacga tttattccca gtgagtcatg taagtgagaa atgacaataa
    22321 atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc
    22381 tccattatca ggctagcgga taactttatc aaaaagcgtc cgtgaatgaa gaagcttttt
    22441 taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag tggaaaaggg
    22501 ctttttttta ttttaaggtg gtggttttca tggaatctga tattagtggc attactaatt
    22561 gctgcaacgt ttatatcgtc aatcaaaatg ataattattg gaggaaaaat ttatggaaga
    22621 aacaaaaact tattcatgga aacaactatt gatggtcagt tcacttatct tcgggatgtt
    22681 cttcgggtca ggtaatctaa tctttcctgt ccacttaggt caaatggctg gcgcaaactg
    22741 ggtcagtgct ggcgtaggat ttgctatctc aggtgctctt ttcccattac tagctatctt
    22801 agctgtagta gtgacaaaaa gtgatggtct gtatgactta gctaaacctg tcggaaaatg
    22861 gtatgctgcc ttgttcttaa tccttgttca tttgacgatc ggaccatttt tcggtacacc
    22921 acggacagct gctactgctt ttgaaatggc tgctaaacca tttttgcctg aaaaatataa
    22981 ttcattaggt atgtttttat tctctgctct tttcttttta cttgcttatt tccttactgt
    23041 ccatccaaca cggttggtca aatacgtcgg aaagtatcta aactccttgt ttcttgcatt
    23101 attagcaatc atttttgtcg tttctttatt ccatccaatg ggaaatctaa atcaatctgc
    23161 tgcagatact tatcaaacaa acgctttaac aaatggtgtc ctagaaggtt ataacacggt
    23221 tgatgcagta gctttgcttg ccttaagtgt cacttttgtc catgcagtcc gtgaccttgg
    23281 tttcaaagat aaaaaagtca cagaattaac ggcaaaagct ggtagcctga gcgttgcgct
    23341 agaagtcatg atctattttg gacttgttct tctcggtgcg atgagtttga ataagatgtc
    23401 tttatcagaa aatggcggaa ctgcactttc tcagatcact aatttttatt taggtaactt
    23461 cggcgctagc ttcttaggag tgatggtcac attaggtgtc ttcactaccg ctatgggact
    23521 cgttgtttct tttgcgcaag atttccataa actgttccct aaagtcagtt atatgacatg
    23581 gctgcggtta acgactttcg tctcttttgt cgttgcaaat gctggtttag acaatatcat
    23641 ccaatggtct ttaccagtat tgatgctttt gtatccattg tcgcttgcgt tgatcttgct
    23701 ttccttaaca gcaaaatttt tccaaaagac accatttgtc tatcaagtaa cgatgttgtt
    23761 tgctgctgtt cctgccgtat tggatatgct ggcaaattcg cctgctttag tgagtcaaca
    23821 aagagttgta gcaagcatgc tcgaatttta tcatcatcac gttccttttg ctgcattagg
    23881 acttggctgg atggtcccaa cgttacttgg ttacgctggc agcctactgt tttattacgc
    23941 ctatcgccta tcagggtata aacaagaagc aaatgaacta ccagaagaat aaccaaatca
    24001 aacacaattt aacataagaa atattaaaca ggaaaaaacc agctagatga aagaatccta
    24061 ttctttcgtc tagctatttt ttagttctct tttttacgcg cacaaaaaaa ctgcggacaa
    24121 tgattgtccg cagtttaaat gggctctata atatctagtg ttggtgacgc cttctgttgc
    24181 caatactttt tcttttttct aataaacgca aacagacacc ttgaaatcct ttagaataaa
    24241 gtcgacgaaa accattcaaa ggagtgtttc aagatgtcct attcagagtt taccaaagag
    24301 ttactagata ttctagacct aaatcttatc tttcatgaag atgcctttcg caaagagcga
    24361 atcaatgacg aaacatgttt tgtttttgat ggcacattga cctatcaacc agaggaatgc
    24421 ttccattgtc actatcaaaa taagcaaacg atcattaaat
//
LOCUS       DAZKOW010000035        24174 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000035 DAZKOW010000000
VERSION     DAZKOW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.035"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            300..464
                     /locus_tag="ACG912_001337"
     CDS             300..464
                     /locus_tag="ACG912_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512669.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            461..691
                     /locus_tag="ACG912_001338"
     CDS             461..691
                     /locus_tag="ACG912_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512670.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQQNIERWIDR"
     gene            876..1421
                     /locus_tag="ACG912_001339"
     CDS             876..1421
                     /locus_tag="ACG912_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0512671.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            1518..1739
                     /locus_tag="ACG912_001340"
     CDS             1518..1739
                     /locus_tag="ACG912_001340"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFQ0512672.1"
                     /translation="MCQECKRRGRNTRGTIIHHIVEAREDLSLFWSVDNLECICVACH
                     NREHPERSGGKKKPKPKSHIVKMYSTPER"
     gene            complement(1896..2060)
                     /locus_tag="ACG912_001341"
     CDS             complement(1896..2060)
                     /locus_tag="ACG912_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HFQ0512673.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            2162..2683
                     /locus_tag="ACG912_001342"
     CDS             2162..2683
                     /locus_tag="ACG912_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFQ0512674.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            2670..3110
                     /locus_tag="ACG912_001343"
                     /pseudo
     CDS             2670..3110
                     /locus_tag="ACG912_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            complement(3150..3308)
                     /locus_tag="ACG912_001344"
     CDS             complement(3150..3308)
                     /locus_tag="ACG912_001344"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HFQ0512675.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            3401..4162
                     /locus_tag="ACG912_001345"
     CDS             3401..4162
                     /locus_tag="ACG912_001345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFQ0512676.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            4349..5701
                     /locus_tag="ACG912_001346"
                     /pseudo
     CDS             4349..5701
                     /locus_tag="ACG912_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            5717..6937
                     /locus_tag="ACG912_001347"
     CDS             5717..6937
                     /locus_tag="ACG912_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0512677.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            6972..7649
                     /locus_tag="ACG912_001348"
     CDS             6972..7649
                     /locus_tag="ACG912_001348"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFQ0512678.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            7646..8839
                     /locus_tag="ACG912_001349"
     CDS             7646..8839
                     /locus_tag="ACG912_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFQ0512679.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            8854..9180
                     /locus_tag="ACG912_001350"
     CDS             8854..9180
                     /locus_tag="ACG912_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFQ0512680.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            9184..9519
                     /locus_tag="ACG912_001351"
     CDS             9184..9519
                     /locus_tag="ACG912_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFQ0512681.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            9516..9911
                     /locus_tag="ACG912_001352"
     CDS             9516..9911
                     /locus_tag="ACG912_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFQ0512682.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            9908..10279
                     /locus_tag="ACG912_001353"
     CDS             9908..10279
                     /locus_tag="ACG912_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512683.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            10295..10855
                     /locus_tag="ACG912_001354"
     CDS             10295..10855
                     /locus_tag="ACG912_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0512684.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            10980..11291
                     /locus_tag="ACG912_001355"
     CDS             10980..11291
                     /locus_tag="ACG912_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512685.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            11315..11506
                     /locus_tag="ACG912_001356"
     CDS             11315..11506
                     /locus_tag="ACG912_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512686.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            11506..15354
                     /locus_tag="ACG912_001357"
     CDS             11506..15354
                     /locus_tag="ACG912_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0512687.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            15355..16227
                     /locus_tag="ACG912_001358"
     CDS             15355..16227
                     /locus_tag="ACG912_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage distal tail protein domain-containing
                     protein"
                     /protein_id="HFQ0512688.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            16224..17273
                     /locus_tag="ACG912_001359"
     CDS             16224..17273
                     /locus_tag="ACG912_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512689.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            17277..18101
                     /locus_tag="ACG912_001360"
     CDS             17277..18101
                     /locus_tag="ACG912_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512690.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18119..20179
                     /locus_tag="ACG912_001361"
     CDS             18119..20179
                     /locus_tag="ACG912_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0512691.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            20196..20345
                     /locus_tag="ACG912_001362"
     CDS             20196..20345
                     /locus_tag="ACG912_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512692.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            20342..20788
                     /locus_tag="ACG912_001363"
     CDS             20342..20788
                     /locus_tag="ACG912_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512693.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            20790..20927
                     /locus_tag="ACG912_001364"
     CDS             20790..20927
                     /locus_tag="ACG912_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0512694.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            20962..21204
                     /locus_tag="ACG912_001365"
     CDS             20962..21204
                     /locus_tag="ACG912_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HFQ0512695.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            21220..21417
                     /locus_tag="ACG912_001366"
     CDS             21220..21417
                     /locus_tag="ACG912_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0512696.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            21428..22444
                     /locus_tag="ACG912_001367"
     CDS             21428..22444
                     /locus_tag="ACG912_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0512697.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSSQVAN
                     NQYILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSY
                     ANANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLW
                     VTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANP
                     TLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYR
                     PDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLN
                     IPKR"
     gene            22559..23767
                     /locus_tag="ACG912_001368"
     CDS             22559..23767
                     /locus_tag="ACG912_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512698.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            23751..23921
                     /locus_tag="ACG912_001369"
     CDS             23751..23921
                     /locus_tag="ACG912_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512699.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT         8438 a         4055 c         5284 g         6397 t
ORIGIN      
        1 tgtctatatg attggattta aaaatgggca aaataaaaga tagtagtaac taagaaagaa
       61 atttttatat tgtcactgtg gcggaaaggg tagacgctaa gcatgtgtgc taggtcaatg
      121 cttcggcaac tatgcaatgt tcgattcatt gccagtgact ttggcaaccg aggcatcggc
      181 ggtttaaaaa tataggggtg cgcaatttcg tacgcgtttt gtgcatcgtg taggttgcta
      241 ttacatatta gatcactcgt tgagtggtct ttttatttta cataaaggag gtaacaacga
      301 tgtatagacc acaatactta gaacagaagt atgaagtaat cactgtgcaa aacggtaacg
      361 gtgagatagt acgaaagtat agaagaccaa taaagagcga tacatataaa cgaaaggaaa
      421 acaatgaagt tatttctttt cgcagaagga gaaaagccaa atgagaaact actggtatgt
      481 atcgctaact aatgaatatc ctcgaaccat tgatgattgt tcagtgcgtg ttgtgcgttc
      541 tgtacaaatc aaagggaagt actctattgt cgaaatgcta agagaagcta caccaaacga
      601 agtggataaa tgcaagctga tatattgcgg tcatggctat tggaaagacg agtatatcca
      661 acaaaacatt gagaggtgga tagatagatg agttacctcg aacatttgaa acgttgctac
      721 atgcattcta agaataaact tcctgacagc tacactgtag atgatgttgc tatacatgta
      781 ttgaagactg agagccatag cagtcctgat ggtagcagta aggagcagac gctcgcgtgg
      841 ttcaagttct ttaagtggat taaggaggaa gagtaatgca catgcacata ggagaaaaga
      901 tatataaatt gagacggctt aataacgaaa caatgaacga cttagttaaa caaactggag
      961 tgtcaaaatc atggttatct gatattgagt ctggaaaaaa gcaacgcgtt gattttaata
     1021 agatatgtgc aatagccaaa cattataaag ttagcttaga atactttcgg gatgattatt
     1081 tggatagtga tgaacacgag gatattattc ctttgacagg cgaaacaaga gttaaatcag
     1141 tgaaatcgct tgaacaaagc ctaaagtatc tgaataacaa acaagaacct aaccttctag
     1201 agatcaaact aaaagatact gactcagtac cagaggtttg gtacaagggt gagaggttgg
     1261 atgaattgcc taaaggatta gtagatgtct cgtatcattg gaagactgat gattttacta
     1321 atgatgatag aggagcgaac gacatcacga ttcaatactt ttctagcttt aatgataagt
     1381 atccagataa gaaaacaatc ggacacaaga gagatatgta aatgaaagaa gttagaccta
     1441 gagacgaaat agacaaacta tacaagacca aacgatggcg agacctaagg caagtagtaa
     1501 tagctaggga cttcggcatg tgccaagagt gcaagcgtcg agggcggaac acaaggggca
     1561 cgatcatcca tcacatagtc gaggcgaggg aagacctgtc actgttctgg tccgtagata
     1621 accttgaatg tatctgtgta gcttgtcaca acagagagca tccagagaga tcaggcggga
     1681 agaagaaacc aaaacctaaa tcacatatcg ttaaaatgta ttcaactcct gaaagataag
     1741 tttgcagcga aatgaaggta gcccccctac tctaaaagat taaagagtaa ggcttgagaa
     1801 gaacggtgct gtccttcctt cgtaaaaaga ccgcttttca agttttttgg agaaaaagga
     1861 aaaagccgac caatttaagc cggctttgga cgaagctatt tcttagtcca tttgtttcct
     1921 ttttgagaag tagggggtaa tcggtcgcct ggatcaattt ttacttcttg tccgccttgg
     1981 acatttccac cacgaggacc cacttctttg taggttcctt ttggtttgtt gtcttcgccg
     2041 ggtttataga gttctcccat aggaatccct ccttaacaaa tttcggcaca gcactgccga
     2101 taacctaatt ataaggattg tgataacgat ttcaatctat cttttgaaag aaggtgatat
     2161 tatgccgcaa ccagcgaaga gtgcaaaatt acaattatta aacggaaacc caaataagaa
     2221 gaataccgaa gaactccgca agcgagcggc cgcagaagac aaattaaaaa tggctactga
     2281 caaaatcaaa ccgccgttat ggctagattc gctaggaaag gatacctttg agtttatcgc
     2341 cgatgaattg ctgtctgtgg atttaatcag taatccggac gtccatacaa tggctctata
     2401 ctccaattgg tattcgcaat acgtttcttt agaaaaacag cttcgaaaac tacaacgaga
     2461 gtacaagttg aactatgcgc ttgcgaaaaa ggaggcagag gcgagaggtg agccgtttaa
     2521 tgaacctaat gaattaattg gtaatccgct ctctcggcag atggatacag cgtctcggaa
     2581 tctccgttct tttggcgctg atttaggact atcaccagca gccagagcta agttagctat
     2641 taagatggct gatgatggtg gtgatgacga tgacgacttc taatattttg gatatgtctt
     2701 acacagaacg tgtggactat tggcaaagct atcttgagga gcaagcttct tggggtggct
     2761 ttttaaaatg tccatatccg gaattgttaa ctacttggta tgcggaacga ttaatcgatg
     2821 gaagcatacc agccagcaaa gaaaatattc aagctgctaa acggcatatg cgtgatttgc
     2881 agcgccaagg aacagatgat tttccttgga tctttgacga agaaaaaggt caccggccta
     2941 ttagatatat cgaaaaaaaa tgtaaaccaa ctgaaggcga ctttggttcg tttgttttgc
     3001 aaccttggca gcatttcata attggatcca tgtacggatg ggtacatcgt gatacaggag
     3061 agcgtcgctt ccgcgaggct cttatttttg ttggacgtaa aaacggtaag ttttgccgtt
     3121 taaaaatcgg gcaaaatcgg tgaaaacctt tatttttggc tttcttttag taataattcg
     3181 atagctttat caagcaattt agacatagga accatagttt cttccgacat tttttttaat
     3241 ccttcgtata aatctttatc aattgcgtta gaaattctaa ttctgttttt tagtccgtat
     3301 ttgtccatgt gtgacactcc tttttatttt actataacat ccgattccac cgcttgcaac
     3361 taccatcaat tgatggtagt atataaaaga ggtgagtaga ttgaaaaaat acattattta
     3421 caaacactta aataaaataa atggcaaaat ttatatcggt gtcacaaatg atattggcag
     3481 aaggtggagg agcggtggga ttgaatataa accaccaaag aacgaaaccc aacaccacag
     3541 aagtttttgg aatgcaatac aaaaatacgg gtgggaaaat tttgatcatt tgattattga
     3601 agaagactta acgatgaaag aagcttttga gaaagaaaag ttttatattg aactttatga
     3661 ctcaactaat aagaaaaaag gttataacat cgcaaaaggc ggaaatggcg gaataatata
     3721 taaagttcac cccaaaggaa tgctagggaa aaaacaatct aaagaatttt cttcaaatca
     3781 cagtaaatgg gcaaagaacc ataagaataa ttgtatgaca aacggtgatg ttgtatgggg
     3841 tgttactcac gaacacccaa aaggcatgtt gggaaagcac cacagcaaag aatcaattat
     3901 gaaaaagaaa gcatatagtg gtgaaaatgc tgtaacctca aaaccaattg tcgcaattga
     3961 acgggatgga actaaaagag aatttcacag cgctaagctt tgcatggctt actattcgat
     4021 tagtacatgt gttttttacc gacttttaaa agatggcgcc ccttatgtga taaatcctaa
     4081 agcgaactac agaaataaag aaaaaatatt agctatcgaa ggaattatgt tcaaacataa
     4141 agaagatacc gaggtaagtt agtagattgc gaaaggttac taactaccgt aacgcgtagg
     4201 agttgaataa atataatgct cccaagagtg tccgacagca gataatttgc tgaaaatgta
     4261 cgctaaactg ggccagaact gactgaccga tgaaaatgag ggtgacctcc agagtgcgag
     4321 ataaaaagct cgcagataat aacaatcgaa aacatcacta atttcaggcc tttccacata
     4381 catggtcgct tatgatgatg aacaaggcgc caacgtttac gtattggcaa atgctcgtga
     4441 tcaagcaagc ttgttgtttg ataaggccgc agaaatggtc aaacaatcgc cggcgctctt
     4501 taagaaattt ggtaagccta aacgatcaag tattaattat gctcccgcct tttctaaaat
     4561 ggaaccacgc gcctcagata gccggaaatt ggatgggcta aacactcact ttggtatttt
     4621 tgacgagatc cacgagttta cgaattacaa tctgatcaac gttatcaaga aatcaagagg
     4681 aaccagaaaa cagcctctga tagtttatat cacaactgct ggatatgtat tagatggtcc
     4741 gttgatgtct tattttgagc aaggtgtgga ctgtttggaa catttggaag atgacatcga
     4801 tgaacggact ttctattatc tggcaaaact tgacagtgcg gaagaggctg atgacccaag
     4861 attatggatc aaagccaatc cgaatatttg tctaatgaat tttgttggca tgctagatga
     4921 ctatgttaag gataaaaaag atccaaaaga atatgctgac tggattacca agcaatttaa
     4981 cttgttttcc gatatcgatg agctgtcatt tgtcgatatg cctaccatta aacgaaacaa
     5041 taaaaccatc gatattgaaa cgctcaaagg taagaagtgt gtcggtggtt ttgacttgtc
     5101 cgaaacggaa gactttaccg cagccgtttt agaatttccg cttgaaacag gcgaggtatt
     5161 cattttgcaa cacacatgga tcccacaagc tagatttgat cgagataaca atcaagagcg
     5221 tatcaaagcg tgggagaagg tgggagatct aacgattatt cctggtgatt acgtcaatta
     5281 tgaatacgtc ttaaattggt ttgtagaaaa ttcgaaaatc tatgacattg taaaaatcaa
     5341 ttatgacaag gccaaggcgc tacgattaaa taaagaacta gaaaatgcag gatttgaaac
     5401 caacgagatt cggcaagggt ttctatcatt aggtgggcca atgcaaaact tcaaggaaat
     5461 gctattggac ggtaaggtga ttttcaacaa ttccaagctt taccgatggt atctatccaa
     5521 cgtcaagctg gtgatggatc gcaactcaaa ctggatgccg tctaagcagt ccaagagtag
     5581 aaaaatagat ggttttgcag caagtttgaa cagccacgcc gaagtgttga atatgttggt
     5641 taatcctgtc ggaaccggga aagtaaccta ttactcgatt tccgatttaa tgaatatgta
     5701 agaaaggtgt ggaggaatga gtattttaga tcgtttgcgt tcttttggcc gagcgaagcc
     5761 gaaagcgagc aaacaagagt attttttgaa tgacccggga ttgataccgt atttagtcgg
     5821 aaaagatgaa atatcagaag ggattttttc cgtaattagc cgtgtatcga acgtttttgc
     5881 gtctctccct ctcaaaatga tagatgtgga gtttggccaa ccggacgact gtcctgcata
     5941 caacttgttg agcgaaggcc ctcgatattt tacaaagttt gattttttcc gggacgtgga
     6001 agttttgaga aactaccaag ggaatgcgta tgtgcagatt ttccgaaata tcaatggaga
     6061 agtagcagat atggcgttag taaaacctgg tgcttgccat ccagtgattg atatggatag
     6121 cggggagctt tactaccaag taactgcgac tgacaaaggc agttacaagc aagttatcta
     6181 tgtacattac atggaaatgc ttcactttaa acaaccgagg tttggcggct tggaaggtac
     6241 agaccccaca aaagtattaa cgaataccct cggatatgat cgagaagtcc gaaaaatctc
     6301 tttaagtcag cttaaaggaa gtaatgaagg gctaaaagtt aagtttgcta gcaatatgga
     6361 tgaagaagct aaaaaagcta cagttaaaaa cattgctgat ttttatcgac aaaacggtgg
     6421 actacttgtg gaagaaaacg gtgtagaaat cgaacgttta caacgagagc tggtagacag
     6481 caagctttta gatactgata aagtatctcg ctccagaatc gctatggtct acaatgtgcc
     6541 ggagcatttc atcgggaata accagtcgag ttactcctct caggaacagc tcaatatgga
     6601 atttttgacg tacaatctag taccgaccgt taatcaatat gaagcggaac taaataagaa
     6661 aacactatcg agaaccgaaa aagcaaaagg ttatcgatat aagtttaatg tcgcaagttt
     6721 gctaagagct gacactcagg caaggggaca attctatcaa atcatgcgac gaggcggagc
     6781 atactcagcg aatgatgtcc gaagatttga ggacttgcaa ccgataaata aaaccggtat
     6841 ggatgattac catatttccg gagacctata tccaatcgat atggatccaa cattaagaaa
     6901 aacaacctcg tctaaaagcg tagccgaaaa cggttaggtt tttttagttt gcaccgaagg
     6961 gaggtggaaa aatgaaaaaa gtgacgttaa gcggcgatgt cgtggataac gataccgcgt
     7021 ggctttatga ctggtttggg atcaattgta tctcaccagg gaaaatttct gccgctctta
     7081 cagaagcagc gggggatgaa gtagaacttg atatctcatc gaacggtggg gatgtcctag
     7141 cggcaagcga aatatatacc gctatccgtg cctatccggg aaaggtatcc ggaaatgttg
     7201 tgagtattgc agcaagcgct gcaagtgtaa tcgcttgcgc ttgcgaaccg cttagaatct
     7261 cacctacggc acacatcatg attcataacg catgggtgac cactagtggc aacgctgagg
     7321 aattaaaagc caatgcagaa atgttaagca gtgtggatga gtctattgtt aatgcttacg
     7381 agatcaaaac aggactcgat cggaaaaaac ttgctgattt aatggcgaaa gatacttggt
     7441 taaatgctca aacagcagta gcagaagggt ttgcagatga aattatgttt gcagaagcac
     7501 cagtaacggt actcaatgcc tctcaaccgg ttattccaaa aaacgcagta actaagttga
     7561 aaaatttaat actcaaagcg gaagcaccgc aagaagaaac actcttacag aaaaaactaa
     7621 aagccttaaa tggagggaaa aacgaatgaa tttagaacaa ttaaaaaatg cgtgggtcga
     7681 ggcgggaagt aaagtctctg acttaaatgc acaactcaat gcagcattgg ttgacgatga
     7741 aaaaacagaa gaagatgtag taagtttgca agcacaagta aaagcagcac gggctaaacg
     7801 ggacggattg aaagagcaag tggcaaatat ggaagccgaa caagtcttaa acgtcaaaaa
     7861 agaaccatta gataaaaaag atgaaaactt gaaaaacaag tttatcaaag actttaaagc
     7921 gatggtcaat ggtgatcctg ctattatggc tactttgaca tctaatacgg atgaatctgg
     7981 taatgctatc ggattgacta ttcctgtaga tgtgcaaacg actattcata ctttggttcg
     8041 tcggtttgac tctttgcaag aatacgtaaa cgttgaaaaa gtgaccacta ccagcggttc
     8101 tcgcgtttat gaaaaatggt ctgatattac accgttgact gctttggata ctgaagacgg
     8161 tgaaatccca gcaaatgatg atcctgcact ttacttgatc aaatacttga tcaaacgcta
     8221 tgcaggtatt tccacagtaa ctaacagctt gctaaaagat actgccgaaa acattttggc
     8281 atggttgtct aaatggatcg cgaaaaaagt agttgttact cgcaatacaa aaatcttggc
     8341 agctattgat ggaatcaaag cggcacaaaa gaaagatgtt acagatgttg atggaattaa
     8401 agatatcgta aacgtccaac ttgatccagc tatcgaagct acatctatgt ttattacaaa
     8461 ccaagatggc ttcaatgttt tagataaagt gaaacgtgct gatggatctt acttgttaca
     8521 aaaagacgta gcttctgcaa ctggatatac cttcttaggt aaaccaatca agaaaattgc
     8581 ttctcgtttc ttgccaaaca aaggtacgca agctactcct aaatatccac tgtacattgg
     8641 tgatctgaaa gaagccgtta cattgtatga tcgcgaaaac atgagcttgc tgacaacgaa
     8701 tattggtggt ggagcttttg aaacagacac cactaaagta cgcgtcatcg atcgcttcga
     8761 tgtgcaacta gttgatgatg aagcggttgt tttggctact tttacaacta ttgcgaacga
     8821 gacaccggcg aaagtttaag gagctgattc cttatgattc ttgatcctaa aatggattta
     8881 ggcgaaatca aaaacgcact aaagattgat accgatgatg acgatgtgga agtaagccgt
     8941 gcggcacaag ctgcaattgc atacattaga ggggctatcg ggaatgataa gccctctttt
     9001 tataagcaag aaaatgaaac ggttgatctg attaatttag ctatcctgca attagcagat
     9061 cattattaca acgctcgatc agcaaccgtg agtgggaact tgcgagagta cgatttaggt
     9121 tttacaagcc taatcttgca actcaaagca agttatttgc tttttgtgga ggaggagtag
     9181 cgtatgcccc ttatccaaac aggaaattta aatcaacgca tcaagtttgt ccgagatacg
     9241 acggttaagg atgaggacgg gcaagttgtc ccgacttcta caaccattct tacttgctgg
     9301 gcaagtgtgc agacacaacg cctgaacgat attaagacgt cgattggtac ggctttggaa
     9361 ggaacactga cgttcattat ccgctaccaa caaaaatcag agctgaccaa tgatatgaaa
     9421 gtgcgttgga atggaaaaac gtttgaaatc attacgatta cgaaaggcga gtttgcgaag
     9481 gacttcacga caatcattgc aaaagaggtt tcaaaatgag tgtagaagtc gatgcaaccg
     9541 aagtgtacaa agcgcttagg gaagtaaaag caaacgttca acgagtggaa agcccagcac
     9601 ttagaaaagc tggggagtac gctcaagaag agttacgaca aaacacacct tactgggatg
     9661 gaacgaagtc aaacggtaaa cgtggttcgt atatgcaaga acatgctaag aaccatgtgg
     9721 ttacaagctc gataaaaaac ggattggtag aagtcggcta tgacaaagat gtttcttggc
     9781 ggatgcactt tatcgagttc ggaacaatca aacaacgtcc aaaaggtttc gtacaaaaaa
     9841 cacaaaagca aatcgaaaaa caagtaacac aaatcattgc tgacgaagta aaaaggaggc
     9901 taggactttg aaaacggcag tatcacaagt ctattcaatt ctgaatagca atgaaaaaac
     9961 aaagaacatt gatttttaca ccaatagtgt tccggaatca gctcaaacag tacctagcct
    10021 tccagttggc agaattacag agatatccgg caactatgaa gattttgcaa gcaacaatcc
    10081 tttgaccatt caatttaacg tacaggtaga tgtatgggtg tcaaccatga aagaggttga
    10141 tgccttttat tttgcccttg atgaggttat gagggggaat ggttggcaat gcgcatacac
    10201 ggaacaaaca gatgacgagg acttggaagg tgcaaagcgg attatcaaac gatatgtagc
    10261 aaatatttca ctaaattaaa aggagagaaa atagatggca acagtaggat tcgagagcgt
    10321 catttttggc gtaaaaacag gtgcaggcgg cactctaaaa gaattagtag cagataagtc
    10381 gaaaggcgga gcgatcgaag ctaaaattac tggattaggc gcaacttcta acacaacata
    10441 cgcttcaaac gtaccgttct tcattgcaag taaaggggtt tcgtcgccaa aagttacgct
    10501 tgacgtggca gacttaatgg ataacggcat ttacagcgaa atcattggtg ctaaaaccgt
    10561 ggatggtgca aatgtaattg gttcagaaac tgaagcgcct tacgtgtcgg tagtcatggt
    10621 tacagcgaac aaagaaggaa aacgcttgtt catgggattg acaaaaggaa aattcagtca
    10681 tccagatatc gacatgaaaa cagctgaaga caaaggggta gaattgcaaa ccgattccat
    10741 cgaaggggaa ttcatttctg atgaacgtgg ctatgtatac ttaacagccg tagaatcaga
    10801 aggaatgacc ttacaaaaat tcaaggactt ggtaaataac aaagaggggg agtagttaac
    10861 cctgcatcta caccaacgac agatacaggg acaccaaaag aaccagaacc aaaaattgat
    10921 acacaaggtt agccattttt ggctagcctt attttttgta aaaacaagga ggaaaacaaa
    10981 tgattgaatt gcaattgaaa cttgacggaa agaaaaaaac attcaaacaa caagatattt
    11041 ccgcacgtgc aatgcgtgag tgtatcaaat tttacgagaa agcggaaaaa gcagacctaa
    11101 ctgatttaga agcgattgat tcaatgattg caattacagc agatattttc caagatccag
    11161 cagttacatt tgatgctatt ttagacggtt tgactgcgag cgagttagta ccggcattag
    11221 aaagtgtttt tgaacaaata aatgaactgg gaaacaatga aaaaaagcag acggcgagca
    11281 aaaagaaata agtttttctg aagctaggaa agcaatggat caaatctaca aagatttaat
    11341 cgaagcaggt tggacgatga gagatgtgga cgaagccgac tatcattatt tgttacacct
    11401 ttttggagaa gtggagagtg gcgaagaata tgtagatggt gctgatttca tcaaacaatt
    11461 tttatcggct gaagacttag taaaacttga ggaaggaggt aaataatggc aggaaaagga
    11521 caaccggcag gaaatatcaa gctagggatt agtttagata gcactagttt tggtaacacg
    11581 ctggacgaaa tcaatgcgaa agtcaaacaa gctgagtcga atatgcgtgc caatctaaag
    11641 gcttatgatt cagcaggacg ttcatacgaa gcacttagtc aaaagacgaa agacttgtct
    11701 acggttatgg aagggcaaaa cgccaaagta agagaattaa caaagcgccg tgatgaagcg
    11761 attagcaagt atggcgagga atcgaaacaa gttgctaacc ttaacacaca gataaacaat
    11821 gctaccgcaa aatataatgc ttacagtcgc cagttgaacg acacaaaaaa agaattggtg
    11881 tattccaaaa cagccgtcaa tgatttatct aatgaaatca aagaaaatga acgacaaatg
    11941 aacgccgaag taaaagcgtt gaaagccgct ggtgatgaat ctggtgcttt tgaagcaaaa
    12001 caaaaagggc tagccaaaca aacggaatta tccgagaaag ctatcgaaga acagcgcaaa
    12061 gttgtgaaac tgatggctga tgagtttggc gattcagcaa atgaaaccga agatgcaaaa
    12121 agggcattag aaaagttaga acgacaaagc caaatatcta gcaggcaatt agaagcactc
    12181 aaaagctcca gcgatcaatc aggaaaagaa atagaagatt ttggcgacaa gtccacaagg
    12241 tcagctagga aactggacgg actaaaagac aaattaagct cgctaaaaag cgcattttcg
    12301 tttggtgcag ttgctggatt agcgcataac gctattagca gtgtagtaag tggcgtgcaa
    12361 ggtttggttg gcgaagcagt aaacgcatcg gattcattga tgaagttttc caaaaccatg
    12421 gagtttgcta actttgggaa gtcacagata gaaagctcga aaaaagaaat gaaagactac
    12481 gccgataaga cggtttatgg tttagaagaa attctgaaca caaccgcaca attggcatct
    12541 aatgggattc ctaactatac agaactaacc aaggcggcag gtaacttgaa tgccgttgca
    12601 ggcggttcta gtgatacatt caaatccgtt gccatgatgc taacgcagac ggcaggagct
    12661 gggaaactaa caactgaaaa ctggaatcaa ttagcagatg cgataccggg tgcttcagga
    12721 ttgttacaag acgctatgtt gaaaaacgga gcttatacag gaaacttccg tgatgcaatg
    12781 gcgcaaggtc aaatcacttc cgacgagttc aaccaagcaa ttgtacagtt aggtatgaat
    12841 gacggagcag ttaaggcagc cacttctaca gacacattga gcggttcttg ggagcagatg
    12901 aaatccactg taataaatgg gctacaaagt attatagaaa aaataggcgt tgaaaatatc
    12961 actggtttca tcaacagagt aacaaaaggg attgaaaatt ctattcctaa aattactcaa
    13021 tttataggtt ggttgagaga tattggaacg tggatcgttg aaaatagaga gccactaaca
    13081 tggattgtcg gaatcatagg cggaattaca ttagcagtaa aagcattgaa cgtagcaagt
    13141 atgttgctgg caattactgg cggaacattg gcagcccctt ttgtggcgat tggtgtagca
    13201 ttaggcgcac tagcaggtgc tttggtgata gcttatacaa aatctgaaac atttagaaat
    13261 atagtcaatg cggcttttac agctgtgaaa aacgtagtta tgagcgttat caataatttg
    13321 gtggaatact acaaaatgtt gtggagcgtg ttgcagtggc tttgggaaaa aataaaagaa
    13381 tgggcttcat ggattggcaa taaattcatt gaaatgaaga acagcgttgt gaatacggtt
    13441 cagaacctat ggaatggcgt gaaaaacttc ttcagcaatg gcgttggaga tacgtggaat
    13501 aaggtagtcg gttgggtaaa aaacattttc aacaaagcaa ctgaattgaa gaacaacgtt
    13561 tctgatgtaa tcggtaacct gtggaatggt atcaaagaca cattccgtag aggtatcgat
    13621 acggtattca attggttttc agaactacca acgaagatga agaatgccat tattggcggt
    13681 aaaaacgcca ttgttgatgc gttcaaaagt attttcaacg cagcacttaa agcgataggt
    13741 aaaccagtta acgcaatcat ccatggagct tcatgggtac tagaaaaatt gggcgcagaa
    13801 cctctacaag aatgggatgt accacaatac gctacaggta caccagcagg tgggcaccca
    13861 atcaatggtc caatgatggt taatgatgga cgtggagcag aaacagttat cacaccagat
    13921 ggtagagcct tcattcctaa aggacgcaat gttgttctaa acgcaccaaa aggaacgcat
    13981 gtcttgacag cagaagagac cgcgcaactt caaggttcta aagctcctaa gtatcgttac
    14041 aaaaaaggta ctaacttctt tggtaacatg tgggatagtg tgaaaaaggt tgctggtaat
    14101 gtaggtcaca caattaaaaa cgtagtaggt gacgtgtggg actttatttc agacccggga
    14161 gcgttagcta gaaaagtact tggggggtta gatgtattag gtggattgac aaagtatcca
    14221 ttagaagtag gtaaaggcat cctatctaaa gcaacaagtg cactgactga aaaaatcact
    14281 gggttgttct catctggtaa cttagatacc tccgtaggaa caaatggcgt ctataaatat
    14341 ttggctgatg ttgctaagtc tgtgatgaag aaattcccag gatttgtggc aactagtggg
    14401 tatagaccag gtgaccctta ttcacatggt aaacgtaatg ccattgatat tgcattacca
    14461 ggcgtcacag gaggctcacc tagatacaca gaagcagcaa actacgcttt tgacaaattc
    14521 gcttccaaaa ttggttacgt aatcactaat gggaaagttc gtgaccgttc aggacaatca
    14581 ggtcaaccag caactggtgc atgggagcca tggcccgatg gagatcacta tgatcatgtg
    14641 catttaaacg gtgtgaaaga cccacaaaac actcaaattt caggagatag cgtgggaggc
    14701 agtggggtag aaagatggcg caatgtagca attagagcgt tgaaaatgac cggtcaatac
    14761 agtactgcaa acttaaatgc attactaaat caaatgcgta cagagtcaaa tggtaatcct
    14821 aatgcagtta acaattggga tattaacgcc aaaaatggaa caccatcaaa agggttgctc
    14881 caagtgattg acccaacatt cagacagtat gcaatgccag gattcaacag caatattttt
    14941 gacccactat ctaacatctt agcttcaatc agatacgcac tatcaagata tggctcacta
    15001 acaaatgcct atcgtggagt tggttacgca aacggtggaa ttgtaaacca acatcaaatt
    15061 gcggaaatcg cagaaggaaa caagccagaa attattattc cgttagataa ggctaaacga
    15121 tcaagagcga tgcaattatt gtctatcgca atggacaaac taggagtttc tcctaaaact
    15181 tatggaaatg cgacaactgt ttccagtgat tacgaaatcc tagaagccat tgaaaaacag
    15241 gcggcaacaa cgaatcagct gttatcgtta ttgctggcat ttttcaaagg aaatagccga
    15301 actgatagag atttagcttt agatattcag aagatcttgg ttaggaggat gtagatgcga
    15361 actgtattac tgagaaatag aataaacgaa gaaattgatt tgtccacaga agactatttt
    15421 gctactgggt tgagtaatat ggggtttgaa gtaaaaaaag aacatgtggg acagtggggg
    15481 aatttcagag aaagcagtga gagcgttgaa atatctgaat ttcagtcatc tgtaatcatt
    15541 tctgtgcatg ggtttcgaga aaaagaactg tacaattcac ttgtgcagtt cctatcggaa
    15601 ggtccgtttg aactggaatt tgcttttgac ggcgaaacaa tggtaagaag atgcagtcta
    15661 aaatctttaa gtaagactga aatcgatccg aaaacatcgt tactaacaga cactttagaa
    15721 ctatatttca catcaaactg gtattccgta aaacgcgaaa aattaattca gcgaccgaat
    15781 gtagtgaaaa cacgtggtaa ggtttttccg tataaaagat cttatatcta cactcaaaac
    15841 ttgtgggaga aaaaaggtgt attcaaattc aataataatt ctgtatacct aacgaatagc
    15901 aaggaacgga tgtccccgct aaaaattcgt gtgattggga agtgttcaaa tccgtattgg
    15961 gaagtaatcc aaaactcaca aatcatcgca acggatggat acttcataga tatgactgaa
    16021 acacagactc tagaagtatc aagccttttt gaagatacaa cggcgatttt aaaagatatt
    16081 gcaggggtag aatcttctgt ctaccaacaa caggattata caaaaactaa ttttgttcag
    16141 gccccaactg gagaattcag cattgtgttt catgttgggg gggcagacgt agagattgag
    16201 ttatacgagg agcgtgatct gttttgattt tagcagtcac gctttttcat cgtgatttaa
    16261 tgctttataa cgaacattta ttctctaaca gttttgaatt tggtgtggac gagatcaacg
    16321 aagaggctag tagctttacg atggataagt acgttcctgt aaaaacaggc gattttctac
    16381 tagcaaaata tattcctagt ggaaaatttg catattttgg tgtaattacg tcgcaggaag
    16441 acgaaaaaat cagttgtaaa agcttactta gtttagctga tagtgagata ccgactgcac
    16501 gtgtgtcagg agataactac gaagagcata tccggcggtt gattgaatac tatttgctga
    16561 atgatccaac gaaacaacta aaagatattt tagacgtcaa agcagaaagt gcgacctctc
    16621 attcgtatca agctaccgat acgaataaac acaagttaag tgcgtatatt ctcaatggtt
    16681 tcaaaaaata caacgtaaaa tggtatttca agggaattca aaacagaaaa atttatacag
    16741 gcatacgtgc tgtaaatgaa tcaatttaca ttaaagacaa ttcttctgaa tttagcgatt
    16801 gggatgtgtt tgttcaggcg ccgggcgctg gaaacgagaa caagctatta atcgttgata
    16861 aagcaatgaa agatatagag aaaccgataa tactgtcaac atggtatttg gacgaagaaa
    16921 acaatttgac acaagatgga tcgaaagaaa atatcacgaa accaactgtc aatttggtta
    16981 atatctacga tcaaaccgca gaagataaag catcctacga agatgtggca aaatcagagt
    17041 tgaaaggcaa tacgtattcg catgaaatca aagtgaacgt tgtaagaaat gcaaaaaatt
    17101 tgaatgtcga aacgattgaa acagggatgt ttgccacgat ttcttataaa ggaaagatat
    17161 acaagtcggt tttgacagct tggcgaatat caagtgataa ggagtttgtg gaattgactt
    17221 tcggaaacat aagaagtcgt tttatggatt attttgaaga taatggggga taaaaaatgg
    17281 ttagcaatgt ggatggatat caatttgaaa acgtgaaagt aagcgcagaa aatgatgcta
    17341 gactttatca cgttttatat aatcggaaaa atcaggttat tgatggttac gatcagtcta
    17401 tgaatttatc ttcaagcgga ttaacagtaa aagttgctgc aggagcagcg attattcaag
    17461 gtcgtatggt cgttgttcga caagaagaaa gtataacagt tccagcaaac tcaagtggtt
    17521 atatagcatt aacagtggat ttgacacaag aagttatacc tggatctatt cttccagaat
    17581 cggaagaata tgaatggact aataatcaag tcaagctaga atttataaca aaagttataa
    17641 aaggtaattt aaacaatggt gataaggtct ataatttacc actatgctca gttaattcta
    17701 ctggatcaac tgtttcaatc tcaaagatat cggatagtta cgagctgact ctctctaaag
    17761 gagaaatttt gtggaggggg actgcgttaa tgcatgatac tcaaactgtc caaccttcaa
    17821 aaaaaatttg gcagacagtt tcaggttttt tgttagtatg gctcccatac gaaaacgggc
    17881 aagcaattga ggatagatat gtaactacgc ctttttataa ggagcgtgta acttttacta
    17941 atgttttagg agaaattgtt tcaggatttg atgactatca caaaaagtgg tttagtaaac
    18001 gaataaacta taattcgaat actaatatat ttacaggtgc agcaagcaat gcaagtggag
    18061 ataacgcaaa tatggtgctt aggtatatag tttcttttta gttaggaggt atggaagagt
    18121 ggcaaattta gaaattaaat tatctgcaaa taagagacag ccttatctac gtcaccgtgt
    18181 tgttggtaga gttggtgatg gggggctcac aacaatcaat gtacaacttc ttgaggaaga
    18241 tgaaattaca ccttttgtaa ttaatctaaa cggtactttg aaatttgtgg gcgaagtttc
    18301 aaacggtaac tataccgagg gagaaccaga aataatcgat tcgactaatg ggttaattag
    18361 ttacacgttc actaagtcaa atttcagcac gagtcatcaa ttcaaacaag catattttga
    18421 atatgtagat cctaacggca aaaaagtaac ttttcagaac ttcatcatag acgtgttaga
    18481 acgagtggat attaattcgg agcaagcgaa atactatatt tcttcattgg aaaaattaca
    18541 gagtgaaatg caaaccactt tcaatcagtt cattagtgat aaacaggtcc aatacgatca
    18601 aatctactcg aaatataacg aattagtaag attgataaat gaatcagata agcaagtaaa
    18661 tgatcgtatt gatcaaacca atcagcaaat cggcgatctc ggcaagctga aaaagatgta
    18721 cagtaacagc atcgactttg ggggctatga ttattcgggg aatccgaatt tgttaagtaa
    18781 gctatcatac gacttaattg aaaatcaaaa tacttcagct ggaacacttt ctaagggtga
    18841 aaactcgttt aaatataata agatatcagc tgaagtggaa ggtggagtag agttatatta
    18901 taaacgaaga ggtatagcta actggttacc ctctaataaa acgcttgtaa tgactgttaa
    18961 gcttagagct ggagcggact atagtccagt tgacggaaaa cttatactga ttagatatag
    19021 gtatgttgac agtggaactg gcaagattgt tttagactta cctattaaca gtaattcgat
    19081 aactcaggaa tggaaagagt ttagtattac tggaactact ccaacattta gcccacaagc
    19141 ataccatcct tggatacaat ttagggctca agatggggta cttggggaaa tagaaatgag
    19201 ctatgacatc aaaatcgaag aaggctctac agctacacca tatcagccta acttacttga
    19261 tgactcttac tggctaggta aaacaccgtt gggtgagaat ttgataacga atgccaagtt
    19321 cccaattata acacgaagca atcctatttc tggttttgat atttcagaag aattaataat
    19381 aggtgaaact tatacggtat ctttaaaagg gactaagcca gctactaaag aattccactt
    19441 atactacgga gaagcaaatt accagcagtt gcagtatcag gcaactttac ttcctgtgga
    19501 aggattaaca gatgtgtgga gtgccacttt tacagccaga aatactagtg aaacgacaaa
    19561 tttacttaga gtagcgttat ggcaaaaacc taattctgca acatatgata ctgttcaaat
    19621 tgactggctc aagattgaaa aaggcgacac acgcacacca aatatcagtc agtttaaata
    19681 ctttggtgaa ggcttgaaag acagtaacaa tccgaacgac tacagctggg atgtcacacc
    19741 agagtatact gaaaaaagct tgaataatac ggttagtctg actgaaccgc aatccatcga
    19801 aggattgaaa aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga
    19861 cgacaacaaa tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa
    19921 gctttatcgt gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag
    19981 tggtggaaat gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt
    20041 tgagcaactg gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg
    20101 tgcagatgga gccattaaat ggcgaagaaa ctcaagttat ggatcggatt atacctttgc
    20161 aattacttac acgatttaga aaggcgtgaa tcgatatgaa ggcagcatat agaccaattg
    20221 aaccttacgg attcgagcaa atcattgtga atgatgaaga acatttaccg gaagaatgca
    20281 cagaagtcga accaccgatt ccaaattgga aaccgaaatt caattactgg gagggaaata
    20341 aatgaaaaac atttggaaat atggccgtac tggcggagag tatgcaggaa aagtattgga
    20401 cgacatgctt gtatccgttc cttacacaga tcagccaccg ctcgaagggg ttcgttcaga
    20461 tggcgaaccg ctaacgattg ctgatcagat gtttgatcct aaattgaacc aatggattat
    20521 tttagcgaac gcgttagatc acaacgattt aaacaatctc aaagcgatgt acgaggctct
    20581 tgaacatgaa aacggcaacc taaaacagct aaatgccaaa ctcatgctaa gcgatgtagc
    20641 gattaaacag gaaaatactg cattgaaaga aaaagctgac agtttagcac aaatcaattc
    20701 aaaaatgatg cttgcttcgt tacaaaatag caaagacatt tcagaaatta aagagcaact
    20761 aaatccagct tcaaagggag gtgagtagta tgtttagttt tagcgatgtg aaaatgatgt
    20821 atgattgggg ctgttttact gacgatcaag ttcgtctatt cgttccacta tgtatcacag
    20881 atgaagaagc agatagaatt attagcaaag aagagagcgc atcttaagtg atgcgttttt
    20941 ttgttggaaa gttggtggaa catgaaagaa gaagcgctcc aagacgttgt ggagagatta
    21001 gtaagaattg aaacaaaatt agacaactac gaatcactta gagaaaaagc tgatagtgca
    21061 aaagatttgg cagacaaagc ctactcagta gcgctaaaca acgcagaaga tatcaaagaa
    21121 atgaaagcca ataataaatg gtcgtggggt tacatgatcg gtttaggcat tacgatcatt
    21181 ggctatttct tgactaaatt gtaaaggagt taagaagaaa tgattttacc cgataagtat
    21241 tatcaagtca ttaaatggac ggttttaaca gtattaccag ctgcatctgt tttagtagcc
    21301 acgttaggaa aagcgtatgg atggaatgga acagatatga cagtactcac tatcaatgca
    21361 gtagcaacat ttttaggcgt tatcactggt gtgtctgcat ataacttaaa aaaataggag
    21421 gaaaaaaatg aagaagaaaa tcattttatc attgagccta ataatggctc tttttttatt
    21481 gccaattaac gggttcgcct atacgatcaa caatgaattt aatttaggtg taaatgaagg
    21541 tagctcacaa gtagcaaata accaatacat cctattgcat gaaacagcaa acgaaactgc
    21601 gacaggacga aatgaagcgc agtacatgaa acgttcttgg tacaatgcct atacagcgta
    21661 tattgttggc gatggtggga tcgtttacca agttggacaa cctggttatg tacaatatgg
    21721 cgctggttca tatgctaatg caaatagtcc tgtgcaaatc gaactgcaac atacgcacga
    21781 taaagtaacg tttgaaaaga actacaaggc atatgttgaa ttagcgagag attccgctaa
    21841 taaatacggt attccactta cattagacac accgtataat caaccaggaa tcaaatcaca
    21901 tttatgggta actcaaaaca tttggggcga tcacactgat ccgtatggat acttatcaga
    21961 aatgggtgta agtaaggaaa aactggctta cgatttagct catggattta cagacgaaaa
    22021 cccaacgact tctgagaaca agcctgtcat tgatccaacc cgagcaggtg cagcaaatcc
    22081 aactttatca gatggaacga accattctca cattgatcag tttggagaaa tcgaaaatgc
    22141 gaacttacac gtcgctggat ggcacatcgc taactataaa tacgagtata ttttcattat
    22201 ggactacaat actgggaaag aattagctcg agtaagagct gatggaattt atagaccgga
    22261 tgtaaatcaa gcttataata cttctggaaa cgttggctat catgtatctt ttaacatgcg
    22321 caatttccct aataagaaag tatacgtcat gatgcgggca acgaatgatc cagaaggaaa
    22381 cactaaaggc ggtgcgcaag atttccatga caaacgttgg tatttaaata ttcctaaacg
    22441 ataaaaatag ctcctcgttg aggagcagta catagaattg aaaattaacg ttaaatcaaa
    22501 aaaatattta caagatgttt atttaagtag ataataaaag aaaaaagcag gtgaaattgt
    22561 gacagcagaa attggtatta tgaataaaag tggaatcgtg ttagcttcag attcagcatc
    22621 tacgattgga gatagcaaag tatataatac tgcaaaaaaa ttatttactt tggattccat
    22681 gcattctgta ggtatcatga tatatggtaa tgctgaattt aatggtattc cttgggaaat
    22741 aatcattact caatataaga aaagcattgg tagttctgtt tttaatactt tggaagaata
    22801 cgcggataat tttatagaat ttgtcaaaac agcatctttt attagaagtg aacagaccga
    22861 gcaagaacaa atgattgggg tttttcaaaa aataatttca ggtttatttg agagtataga
    22921 agtagatatt aactttttaa taagccaagg tacacaaata gacaaagatg tcctagtgaa
    22981 gttattacaa acaaaaatga ataccaattt gtctcaacag tcccagacat tcattttaga
    23041 tattgaaaag gcgttatttc ttagtaacta tggagaaata ttaaaagata ttcttaatag
    23101 tatttctaca atggaaggtg tttcagaagc tatatcagag gaaattcaaa gttacgttta
    23161 tgaaattata attagagacg atgtttattc ttcccctaca ggaatagtta tagcaggata
    23221 tggaagaatg gatattttcc ctaaactata ttcttataat atgtttggat ttgttatgaa
    23281 tattctgaaa tactcagaat atgaaagtgc tcaaatagga aatgacaatg gttcattaag
    23341 atctactata ttaccatttg ctcagtctga tgtggtaaat actgtagttc aaggggtaga
    23401 tccacaaatt actaattatc tgtcgagtca agtcgatagt tttgacgata acggaaaaaa
    23461 tacctatatt aatattataa agaatatttc agagtttcaa caaaatcaat tcatatttcc
    23521 gttactaaac atgattgctt tactcccagt tgaagaaaca gcaattatcg cagagacttt
    23581 attaaattta accagtttta aacgaaaata tactacttct gtagaaacag taggtggccc
    23641 aattgatgtt ttagctatta cacctaatga tggccctatt tggattaaaa gaaaacatta
    23701 ttttgatatt gataataata ttggatacag attgcgaaag gagagagcga atgattacaa
    23761 taattaaacc tcaggaaaaa tcccctgttt tgaaatttaa tgagagcaat ctagcccata
    23821 ataaaatgag tataactgat acagtatttg atgtaatggt tgagcaacaa aaaaaaatta
    23881 ataataacaa tagacaaaga aaagaaatta accaatatta atgttttttc tgttgcgttt
    23941 tttgcagtca taatttaaaa cctcgctcaa tcttgggcga ggtctttttt tatttttctg
    24001 aaaagttaaa gtaaataata gcataaaata atatatttta caaagaataa gtacaatcta
    24061 gttttttgct attaaatgtg taataataaa tatgccatca caacatgaag aatgaaatcc
    24121 attattatct agtctagtcc attctttttg tttgcagtat ttgtgatggc tttc
//
LOCUS       DAZKOW010000036        24082 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000036 DAZKOW010000000
VERSION     DAZKOW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.036"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACG912_001370"
     CDS             complement(<1..430)
                     /locus_tag="ACG912_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0512700.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
     gene            complement(945..1574)
                     /gene="udk"
                     /locus_tag="ACG912_001371"
     CDS             complement(945..1574)
                     /gene="udk"
                     /locus_tag="ACG912_001371"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFQ0512701.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1658..2353)
                     /locus_tag="ACG912_001372"
     CDS             complement(1658..2353)
                     /locus_tag="ACG912_001372"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFQ0512702.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(2366..3082)
                     /locus_tag="ACG912_001373"
     CDS             complement(2366..3082)
                     /locus_tag="ACG912_001373"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFQ0512703.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            3412..4020
                     /locus_tag="ACG912_001374"
     CDS             3412..4020
                     /locus_tag="ACG912_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFQ0512704.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            4345..4668
                     /locus_tag="ACG912_001375"
     CDS             4345..4668
                     /locus_tag="ACG912_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFQ0512705.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4850..6220
                     /locus_tag="ACG912_001376"
     CDS             4850..6220
                     /locus_tag="ACG912_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFQ0512706.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(6350..7651)
                     /locus_tag="ACG912_001377"
     CDS             complement(6350..7651)
                     /locus_tag="ACG912_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFQ0512707.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(8005..9996)
                     /locus_tag="ACG912_001378"
     CDS             complement(8005..9996)
                     /locus_tag="ACG912_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFQ0512708.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(10140..10406)
                     /locus_tag="ACG912_001379"
     CDS             complement(10140..10406)
                     /locus_tag="ACG912_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0512709.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10916..13042)
                     /gene="pnp"
                     /locus_tag="ACG912_001380"
     CDS             complement(10916..13042)
                     /gene="pnp"
                     /locus_tag="ACG912_001380"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFQ0512710.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(13230..13499)
                     /gene="rpsO"
                     /locus_tag="ACG912_001381"
     CDS             complement(13230..13499)
                     /gene="rpsO"
                     /locus_tag="ACG912_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFQ0512711.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13750..14313
                     /gene="def"
                     /locus_tag="ACG912_001382"
     CDS             13750..14313
                     /gene="def"
                     /locus_tag="ACG912_001382"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0512712.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14566..15087)
                     /locus_tag="ACG912_001383"
     CDS             complement(14566..15087)
                     /locus_tag="ACG912_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFQ0512713.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(15090..15932)
                     /locus_tag="ACG912_001384"
     CDS             complement(15090..15932)
                     /locus_tag="ACG912_001384"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFQ0512714.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15925..16650)
                     /locus_tag="ACG912_001385"
     CDS             complement(15925..16650)
                     /locus_tag="ACG912_001385"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFQ0512715.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16826..17122
                     /locus_tag="ACG912_001386"
     CDS             16826..17122
                     /locus_tag="ACG912_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512716.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      17378..17552
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17690..19282
                     /locus_tag="ACG912_001387"
     CDS             17690..19282
                     /locus_tag="ACG912_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0512717.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            19295..20035
                     /locus_tag="ACG912_001388"
     CDS             19295..20035
                     /locus_tag="ACG912_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512718.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            20203..21552
                     /locus_tag="ACG912_001389"
     CDS             20203..21552
                     /locus_tag="ACG912_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0512719.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21680..22270)
                     /locus_tag="ACG912_001390"
     CDS             complement(21680..22270)
                     /locus_tag="ACG912_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0512720.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22417..23769
                     /gene="brnQ"
                     /locus_tag="ACG912_001391"
     CDS             22417..23769
                     /gene="brnQ"
                     /locus_tag="ACG912_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFQ0512721.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6886 a         4854 c         4561 g         7781 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcaca
      481 tcattagggg gtgcgacaaa agagcttctt caaaaaaata agtcgaaatc tcacacaaat
      541 tcgagaaata attttcgtaa gtttcttctt atttattgca tctgaccact tctgttacaa
      601 cctctcgtta aacggtgttc aaaagcttgc atctacgata ataagcccaa cccaacgaaa
      661 aacgtgagga gctgttttat ttggatgctc ttatcgcttg atgcaaagac agctttttta
      721 caacctctac ttcacggtgc tcaatcagct gaccttcggt attagctcaa aaaacggtaa
      781 aatataaaca gctattttcc gtttcttctt tttaatactt aggtcaagat gctgttttca
      841 caacctcttg ttataaataa aaaaacagtt ggctggtgac tgttcaaaaa gcagaatcct
      901 tgcttttcaa acggtcgcag tccgttcaac tgtttcgctg cgcactattt atggtttaag
      961 aaactagcta cttttgttgt aatcaaatca atagcaacgt ggttttctcc gccttctgga
     1021 acaatgatat ccgcatagcg cttcgttggt tcgataaatt gatgatacat cggtttaaca
     1081 actgtcaaat attgttcgat cacggagtcc aatgtgcgtc cccgttcttc catatcccgt
     1141 ttgatacgac gaatgatacg aatgtcatca tctgtatcta cgtatacttt gatatccatc
     1201 aagtcacgca gacgttcgtc ttctaaaatc aggattcctt ctagaatgat cacttctttt
     1261 ggttcctgaa tgatcgttgc ctgacttctt gtatgcgcta catagtcata cacgggtttt
     1321 tcaatggctt tatagttcag taagtcgcct acatgctgaa tcagcagatc attatcaaag
     1381 gcaaatgggt gatcgtaatt tgtgttcaat cgttcttcaa aactcaaatg gctctgatcc
     1441 ttgtaataag aatcttgttc aagcatcatg atggaatggt ccgggaagtt gttgaaaatc
     1501 gcacggctga cacttgtctt tccgcttccg gatccacccg tcacaccaat gataattggc
     1561 ttgctttttg tcattgacta aaacctcttt cgtatccgtt ttttcattct aatctattat
     1621 agcgaaaaaa cacgaaaatg ctatattttt ctgaaaatta atccttttca atttccaata
     1681 atgaataaag ttcctctaat gaatcgattc gataagtagg ctggatttct tcgatttctg
     1741 gtaatttatt tctattgaac caaatcgtat cgatccctgc catatttcct cctttgatat
     1801 cagaagtcaa agaatcacca atgatcacag tttgcttttt atcaaaatta ggaatgtggg
     1861 aaaagacatg atcaaaaaat tctttcatcg gcttttgcgc tccgacagct tctgagacaa
     1921 agatatcctt gaagtaatca agcattcctg catctgttaa tcgccgatgt tgcgtgtgcg
     1981 acacaccgtt cgtcacaata taaagatcag catgcggatg gacctttttc aggatttcta
     2041 gacttcctgg aacaagatca tgaccttggt tcaagtagtg gcggtagcgt ttttccattt
     2101 gatcactatc tactgttttc ccaaattgct ggaaaagtaa cccaaaacga ctgcctatta
     2161 tttcttctcg tgatagtctg cctttttcaa actcctgcca taaatgatga ttcaaagcct
     2221 tatagctggt ttcgatttct ggtgtcaatg tgattccttg ttctgcaaac aaagcactta
     2281 aagcattggc ttcagttaat tgaaaatcta acaatgtatc atctacatca aacaataaag
     2341 ttttatattt catctttttg acctcctatt taaaataaat acttaacttt ttcatctctg
     2401 ctaatgtcaa cgatcgatac tcgcctactc ctagctgttc atctaattcc aatggtccca
     2461 tgcttagccg tttcaatgcg gtcactttct ttccttgggc taagaacatc tttttgactt
     2521 gatggaattt tccttcttgg atcgtcaata aaaccgtact ttggtgcttg tctgctttga
     2581 gtatttttaa atcagccggc ttacattgat acccatcagc gaatacgatt ccattttcaa
     2641 atgctgttac gtctgccggt gtcacccttt cgttcacaac tacttgataa gtctttgaaa
     2701 cctttttttc tggaagtagc atttcatatc ccaattgtcc attcgaagtg atcagcaata
     2761 gtcctgttgt atctctatct agtcttccca ctggatataa tggttggaaa cgatcggcac
     2821 ttgcaatcag atcgattact gtcgtctttt ctggatctga tacagctgta acagcttttt
     2881 caggtttatt gagaagataa taggactcat ttgtttctaa gtgttcacct gctacttcga
     2941 tttgatgcag taaactatct acatttcgat tttgtcgaaa ttcgactgtg ccatctatca
     3001 tcacttgctt caaagcaaac agccgcttca tttccttgcg tgtcgtttct aatttttctt
     3061 ctattaattt atctaaacgc atgctgtttc ctttcttatt actactaatt aattatacct
     3121 tgaatattct tgcttgcaaa tacatcatca tagttattcc ttgacttaat taacagttca
     3181 cgttacggtt aaatagtaaa gtgaattgga tttctcatac acagcgtaat gacctggtgg
     3241 agaaaagttg aatcctgact ttctaagggg agatgcgcgc atctctcctt ttgcgctttt
     3301 ttaagcgccc tgccttcgat gatgtgcacg agattagcct ttagaattca cttaaataaa
     3361 atctgtccgt taccaaagca gacagtcaca ttttaaggag gaattttggt tatgtcagta
     3421 tcattagaag taagtaaaag agaagtacgt ccccgttcat taagaaacaa acttcgtcac
     3481 gaagggaaag ttccagctgt tgtttatggg tataaagtag aaagtacccc tatctcattc
     3541 gatggtttga cactaagcaa attgttgcgt gaacatggag cgaacacagt catcacgatg
     3601 gacatcgatg gacaaaaagt caatacattg gttcacaaga tccaaacgaa cacttttacc
     3661 aatcatttcg aacatgcaga atttctatct gtaaacatgt cggaagaaac agaagtcgaa
     3721 acagaagtcg ttcttgttgg cgaatcagtg ggtgctaaat ctggtggagt gttagctcaa
     3781 aacttgtata cagtagtcgt atctgctaca ccagacaaat tgcctgaacg aattgaagtt
     3841 gacgtgacaa acttggaatt aggtgattct attacagtag ccgatcttcc aaaaaacgac
     3901 gattacaaat tcgtgaccga cagtgaagag caaatcgctg ctgtagtcgc tcaaagtgaa
     3961 gaagaagaaa gcacaggtga aggtggcgaa ccagaagtga tcggttcaac agaagaataa
     4021 tactgaaaca atcaggtggt gttagaagcg gcgggtcagc agcttttaac acgccttttt
     4081 tatttctaat cgtttatttt catatggggc ttccactttt gtaaatggct ttacaaacga
     4141 aaaatcctaa tttaccaagc ttttcctcgc atacttttat cgtaccttta taaaaattta
     4201 tactttgaaa aaaagtattc aattattgtt gcatttttcc tgattcttta ttagaatgaa
     4261 ccctcgttga agtaagtatt tctagtttat cctaggatta attataaaaa taaaacattc
     4321 gttgattcga aggaggttaa acagatgttt gaaagttttg atagcagccg caaccgttac
     4381 gcttcagttg gtgtcgtatc aagtttgcca gatgaattga ttgacagtat atggtttatt
     4441 attgacctag atcttaaggg agtcattcca ttagaaaata tattaacctt tgatttgatc
     4501 gaaaatcgtg gaaaagtgac gatgcatttt tctcaagcag gaagcaatgt ggaaatggat
     4561 attgatttgc cgtttgccta ttcgaaccaa tttcctgctc aagtatacgc ctatgatgac
     4621 ggtacgagag aaacgatcct tttaccaagt gagattagac aatattaagg tgatagataa
     4681 acgtggatgt cccttatttt acagaccagg ccaagttgtt gattctttga ctcaattctt
     4741 ggcttctttt tatggagttt cataaattat cattgatttc taattggtat tctcaggata
     4801 aacgctatat actactaact aaattttaaa gatataagga gtttaaaaga tgcgcgattt
     4861 aacaaaagga agccccgcta aactgatttt gatgttcact gttcctttac tgatcggaaa
     4921 tgtttttcag cagttctaca atatgatcga tatgatcatc gtgggtcaga cgctaggtaa
     4981 aaatgcgctg gcagcggttg gtgcaactgg aagcctgaca tttttgatca ttggctttgc
     5041 ccaaggttta acagctggtt tagccattat taccgcacaa cgttacggtg caaaagatta
     5101 tcggggtctg aagaaaagtt ttgctgctag tgttgtcatt agtcttgtcg tcactgtgat
     5161 tttaactgtc ctaagtctcc tgtttattcg tccgatgctt caattgatgc aaactcctcc
     5221 tgaaatactt gatcaagcac aaacatttat ttcaattatt ttattaggga tctttgcatc
     5281 agtcagtttc aatttgcttt ctaatgtcat tcgcgcactt ggtgacagcc gcacaccact
     5341 attctttctg atcattgcgg tcatcatcaa cgtcgtactt gatttgatct ttattatcta
     5401 ttttggcatg ggtgtggaag gtgcagctat tgctacagta atcgcacaag tttcttccag
     5461 cgtactgtgt ctagtgtata tcaagaaaaa aatgcctttg ttacagttac gcaaaaaaga
     5521 tttcaaattt gaaaaagaag agtttgctgt acacctaaat gctgctttgc caatggcgtt
     5581 tcaatcttcg atcatcgctg ttggtgctat cgtgttacaa gcagcactaa atagtctagg
     5641 gacagatgtc gtagctgctc aagccgctgc tggtcgtatc gaccaatttg caaatcagcc
     5701 gatgatgtca tttggtattg caatggcaac tttcactgca caaaactatg gtgcgaaaga
     5761 atatggccga atcttgcaag gggtacgtca aactttactg atgagtgtcg gatttagtct
     5821 cttagcaggt gctatcgtca ttttcttcgg tcatttcttt gttcaattgt ttgttagccc
     5881 atcagaaaca cgtgtatttg aattagcaca aacctacttc aatatcaatg ggtctcttta
     5941 ctggatctta gcagtcctgt tcattttacg ctacacactt caagggcttg gacaaagcaa
     6001 gatccctacg atcgccggta tgatggaatt gcttatgcgt tcttttgcag ctatcgttct
     6061 tacaggaatg tggggatacc caggagcagc agcagcttct cctttagctt ggatcggttc
     6121 tgttgccgtg ttgctttact cttatatgcg ttcgatgaaa caattgaaac acatgggtga
     6181 agaggaacat ttcacattag aacatgcaga agcaagatag ccttgattaa aagaaatcgt
     6241 tgcggctaaa aatgaaaact tttattgaaa atatcttctt cgtttaccga aaacaggttt
     6301 tttagataac aaaaaggctg tgacaaaaaa tgtcacagcc ttttttcttt tattctgttt
     6361 ttctttccgt ggactctact ttgaaataat cgatatgtgc atgctggtat tctaataccg
     6421 tgaaacggaa tgatgcaatc tcaatgattg ttcctgcttt tatttcttta tatcggctag
     6481 aagcaaagcc cgcaagtgta gtaaaattcg tttcttcaaa ctctggaatc tctacatgaa
     6541 aataacgctg gaaatcatct aatgttagtt tgccggagat gatataagag ccatctgact
     6601 gttccgctat caatgcttcg tccaaatctg tctcatctcg gatttccccg acgatttctt
     6661 ctaaaatatc ttcaatcgtc actaagccgc ttgtccctcc gtattcatct tttaagatag
     6721 caatatgctt accctttttt cgcatgatcg tcaataattt ttttataggg atcgtttcag
     6781 ttgttacgat cggctcttca atcaactgtt ctactgtcag ctggtcatct ttataagatt
     6841 gcttgatcaa atctggcaag gtgacataac ctaaaacatt gtcttttgat tctaagataa
     6901 ccggatagcg agtatgtcct tgttcaatcg aagcttttat cgcttctttg acggtttctg
     6961 aagcttcgaa tacttgcatg gacgtacggt ctacttgtat atcttttgcc aaggtatcat
     7021 caaattcaaa cacgttttcc aaataataat attcttcttt ggtcaactct cctttttcat
     7081 aagaattcaa tgcgactaat gtcagctctt cttgagacaa tgtctcttct ccttcagata
     7141 cgagacgcac acctaatagc ttccctagcc ctgcagctga atgattcaat acccaaataa
     7201 aaggaaacat tactttataa aaataatgaa gtggtttaac tacgaacaag acgaccttct
     7261 ccgtattgac gatactatag ctcttaggaa gcagttcgcc aataaccaca tcgatatacg
     7321 taactaaaat aaatcccagc gccacagaga ttgtactgct aatagccgaa ctaaaaggta
     7381 gtttaccgat cactggttct agtaaagccg cgacagatcc ttcaccgacc cacccgatga
     7441 tcaaaccggt tagcgtaatt cctaattgac tagctgataa atagttatct aaatggtgtg
     7501 taacatgaag agctagcttg gcgttcttta cattttcctt ttctagttgt tccaatcttg
     7561 atcgtctgat tttcactaaa gcaaattcac ttgctacaaa aaatgaagta attgcaatca
     7621 tcaaaaataa aataatgaaa ttgataacca tattctcccg cctttctctt tttctattgt
     7681 accctttttc tacaaaaaga ttgaaaataa tgcgtattca tatttaggaa taaaaaagga
     7741 gatgcggcat ttttcagcca catctcctta tcacttcaaa atcattgcgt tacttgttgc
     7801 taaacagctt tttcatgacc tctgataaaa acggtattca aaagcttgca tctacggcat
     7861 taagcccaaa cagatgaaaa acatgagaag ctgtttttat ctgctcgttc ttactgcttg
     7921 ttgcagtaca gctttttcat gacctctgat aaacggtgtt caagtagctg acattcgaca
     7981 ttagttcagg tcaaagcgct actttcacaa ccgctctttc aaccatgttt tccgagtatt
     8041 tccaatgata agcggaacag tttcatgttt tacttcgctg tactctagcc caaaccatgt
     8101 ttctggcgag gtagcgattt ttttgattgc cagttttgcc tgcatgattt gacgttgcca
     8161 ttttcctaga gttgtcacat tggaaactac ttcatcaatc aggacaaatt ggaaatcgcc
     8221 aacttctcgc cctggtgtca gagagtatct ttgtggctgt tttggcaaac ggccttgttt
     8281 catcaaatca tggatgactt ggcgaacata tacgttgatt tcctgttgga ccctgaaacc
     8341 taagaacaga ttcacttgga caataaaatc agtgtccatc atatctactt gatattcttt
     8401 cgtatatggc tcatccgtca cttccacatg aacaaaccaa tagactcgtg ctcttttagg
     8461 ccgtttatct aaaatcgaat agatgaactg tcggccaact ttatcttcag ccatatcagg
     8521 aaccaagaag accacatttg tctgatagat tggtaaagaa gtatcatcac gtaattctct
     8581 aagctgcggg atataatctc tcaaagacac atcctcggtt gacgattcgc gaataatgtt
     8641 cccttgttcc caaataaaca tcacgactaa gatgatcaaa ccaatcagaa cggctacaaa
     8701 accgcctcgg aagaatttga caacacttga gataaagaaa acgacttcta tcgcaccgaa
     8761 gaaaaaggta actaaatatg caagccaacg agaatgccca ttttggataa gataaaagta
     8821 aagcagtgct gttgtcatca acatcgtcac agtgatcgat agaccgtaag cagcttccat
     8881 atgatgagac gttcggaatg tgacaaccac tagagaacag gcgatccata aaatcagatt
     8941 gactgctggg atatacattt gcccgatact gttcccagga tacatgatct ttaaccgagg
     9001 cagcagtttc aacttgattg cttctgaaac tagcgtaaac gaacctgtaa tcaatgcttg
     9061 cgaagcaatg actgcagcga ctgttgcaaa agcaacgccg atcacgatcc aactttgagg
     9121 catcatttgg aagaatggat tcaggttctt gatttgctgt gcttcagggt tttgataaac
     9181 attcaacaac cacgcagcct gcccaaaata attgataacc aaacaaattt tgacgtaagg
     9241 ccagctcaga cgaatatttc gtttgccgac atgtccaaga tcggaataaa gcgcctctgc
     9301 acctgtcgta gccaggaaaa tatttccaag aatgaacaag cctaatttgt tatctgcact
     9361 aaaaagcagc tggatagcat aataaggatt cagtgcacgg ataactgtcc agtcttgact
     9421 gaagttcatt aatcccatta cgcctaaaaa agtaaaccaa gcgaacatga ttggtccgaa
     9481 tgcttttccg acaatatctg taccaaagcg ttggaccatg aataaaacaa aaataataac
     9541 taaagtgata atgacgatca cgttctggtt attaccgaac cggtcaaaaa actgcggaat
     9601 tcctcgcaag ccttcaatcg cagtcgttac cgtcacagca ggggtcaata ccccatctgc
     9661 tagtaaggca gctcctccga tcattgcagg aatgatcaaa tatttcccac cttttcggac
     9721 aagtgtgtag agtgagaaaa ttccaccttc accatggtta tctgcattca atgcaatcac
     9781 gacatatttg attgttgtca gtagagtcaa tgtccaaaaa atcaatgaga ctgatccgat
     9841 aacaaaatca ggattgattc ctgacaatcc gccattatct tcaacaatcg atttcataac
     9901 gtaaagcgga cttgtcccga tgtctccgta gacgactccc atcgctacaa gcaggccagt
     9961 cattgatatc tttttggtat tgtgcgttgt tgccacgttt ttccctcatt tcttgttata
    10021 tggatatcct ttaggtaagc ttgaatgata gatgatattc ttatccatga cacccgcttt
    10081 cctaaattgc ttctaattat ccaagtaaca cactgaagtg tcaacttatt tgaataaaat
    10141 tattctttct ttggacgttc tctgatacgt cttgccacaa atgaataaag agcaaggcaa
    10201 aaaattgcac ccaataatgc tggtatcaaa taaaaaccgc cgagttccgg tcccagtggt
    10261 cctagtaaaa attcgccgag ccaactacct aaaaaaccga taatgatatt tccagatacg
    10321 cctcccggga cttcctttcc taaaattgcg ccagctatca aacctaatat accgccgata
    10381 aataacgacc ataaaaaatt cagcatctcc tttccctcct ttatccatat ttctcacttt
    10441 tattttacgc tttattcaga aggactccaa aaatatgcgc caagaaactc cagaaaaaaa
    10501 gaagttgtaa caaaagtgtt cagcttattt ccgtagaagc tgcttctgga acaccgtgaa
    10561 tcagaggttg tgaaatcagc ggtttgacct gagtattaga gaaacaaacg gaaaaatagt
    10621 tcctcatatt ttgccgtttt ttgagctaat atcgagggac agctgattga acatcgttta
    10681 ttggagaggt tgtaaaaaag ctgttctgca tcaagtaata agaacagcca aataaaaaca
    10741 gctcctaatg tttttaactt ggctggactt attaccgcag atgcaagctt ttgaacaccg
    10801 tttattcgaa aaaaaaggac tgtgacacgg cttttgtcac agtccccttt tttcgaataa
    10861 ttattgtccc cctgtcacct tcacgatcac aagtacatta ttcctatgaa ttaaattatt
    10921 tttcttcatt tttcgttgaa ccatcttcgt tcaacattgc ttttcttgac agatttacgc
    10981 gtccttgttt atcgatttca gttactttca caaggatctc atcacctagt ttaacaacat
    11041 cttcgacttt attgacacga tcgtttgcta attgggaaat atggacaagt ccatcttttc
    11101 ctttgatcaa gttgacgaat gcaccgaatt tttcgatacg gacaactttc cctagataaa
    11161 cttcaccaac tttgacttct ttggtcaaat cttcgatgat cttgatagct tttttgatca
    11221 tttctgcatc agctgaagca atacttactt ttccatcttg atcgatatcg attttcacac
    11281 cagtttcatc gatgattccg ttgatcgtat cgccgccttt accgataaca tctttgattt
    11341 tagcaggttc gatctggatc atttcgattt ttggtgcata ttggcttaat tcttcacgtg
    11401 gagcagcaat cgttgaagtc aattcttcta agatttccat acgtgcttgt tttgcttgag
    11461 ttaatgcttc agttaggatt tgttctgtga ttccttgaat cttgatatcc atctgtaaag
    11521 cagtgatacc atcttttgta ccggcaactt taaagtccat atcgccaaga tgatcttcca
    11581 gcccttggat atcagttagg atcgtatagt tttcaccatc agaaacaagt cccatcgcaa
    11641 taccggcgac tggcgctttg attggtacac ctgcatccat caatgctaat gtgccagcac
    11701 agatacttgc ttgggaagaa gaaccatttg attccaatac ttctgctact aaacggatcg
    11761 tataaggaaa ttcttcttct gaagggatta cttgtgctaa cgcacgttca cctaatgcgc
    11821 catgtccgat ttcccgacgc cctggagagc ctgcacgtcc tgtagaacca acagaaaatt
    11881 gagggaaatt gtagtggtgg atgaatcgtt tgctgacttc cacccctaaa ccatcaatga
    11941 tttggtgctc tcctagaggt gcgagtgtac aagctgataa agcttgtgtt tgtcctcgtg
    12001 tgaacaaacc tgatccatgt acacgtggca ataagctgac ctctgatgac aatggacgga
    12061 tttcatccaa tttacgcccg tcaggacgga tcttatcgat ggtgatcaat tcacgtacga
    12121 catctttttc aagatcttct gtgatttgtt taacttcttt caacaagtga tcttcatctt
    12181 catgacctgc aaatttctca gcataagctt cgcggatatc gatcttcacc tgctcgatat
    12241 tatcttcgcg agcaagtttt tcttcagtca ttactgctgc tttcattgta gcgtaagaag
    12301 catcaaagat ttcttttttc aatacttcat caacttgcag taaagtcact tccatttttt
    12361 ctttccctac tgcttgaacg atttcttctt ggaaagcaac taactctttg atagcgtcaa
    12421 aaccaaacag caatgcacca agcatgtctt cttctgaaac ttctttcgcg ccgctttcaa
    12481 ccatattgat ggcttgtttc gttccggcaa cagtcaattc gatatctgtt tgttctgctt
    12541 gttctactgt tggattcaag acgtactcgc catttacacg accaacttct acgcctgcga
    12601 ttgggccgtc aaatgggata tctgagattg atagtgctaa agaagaacct agcattgctg
    12661 ccatagctgg cgagcagtct gtttctacac tcatcacgat gttcgttact tggacttcat
    12721 tacggaagcc ttctgcaaac atcggacgaa ttggacggtc gatcaaacgg gcagtcaatg
    12781 ttgcttccgt acttggacgg ccttctcgtt tgatgaaacc tccagggatt ttccctgctg
    12841 catacatttt ttcttcataa ttgatcgtta atgggaaaaa gtctgtatct tttgcttctt
    12901 tagaggccac agctgcactt aagactactg tatctccata acggactaaa acggctccgt
    12961 ttgcttgttt tgctagctga ccgacttcga tttgtaaggg acggccgccc catgtcgttt
    13021 tgaatacttg tttttctgac ataacttctc cttttccttc tcgtgaaagc aagacgctac
    13081 taaacaaatg aaaactgccg aaagccgtct tttgagcagc tttcatttgg ttagtaggag
    13141 tccatctagc ttgatcacgg aattttactt aattgatata cgagtaaaaa aagtgagatt
    13201 catgtgaatc ccactctttt tgactaaaat taacgacgca agcctaaacg tttgatcaat
    13261 tcgcggtaac gttgaacgtc agttttacgt aagtaagcta acaagttacg gcggtgacca
    13321 acttttttca ttaggccgcg gtatgaatga tggtcttttt tgtgcacgcg tgcatgttca
    13381 ttcaagtggt tgatttcttc agttaataca gcgatttgta cttctggaga accagtatcc
    13441 ccttcatggc gagcgtattc gttgatgatt tcattcttgc gttcttttga aattgccatt
    13501 tctttcacct ctttcgatta ttcccctgcg actgagtaaa acgttggtga ttcgcttaac
    13561 caagtggcag gtcatgtgcc tagagcacac agctatactt tacctgaaac cgggttgaaa
    13621 atcaagtagt taatcattaa gaaaaggtag ttttcaaaca atcttactac tacttttcca
    13681 tttttgtggt aaactataat gcgaaaatag acttgaaaca cagtctttta agaaaggggt
    13741 acatattcca tgattacaat ggatgatatc attcgtgaag gaaatccgac cttacgcgaa
    13801 gttgcaaaag aagtttctct tccattaagc gaagaagata tctctttagg aaaagagatg
    13861 cttgagttct taaaaaacag ccaagatcca atcaaagctg aagaactaca cttacgaggc
    13921 ggtgttggct tagctgctcc tcaattagat atttctaaaa gaatcattgc tgttcacgta
    13981 ccaagtcctg acccagaggc agatggacct tctctaagta ctgttatgta taatcctaaa
    14041 attttgagcc attctgttca agatgcttgt ttaggggaag gtgaaggctg tttatctgtt
    14101 gatcgagaag taccaggata tgttgttcgc catgccaaaa tcactgtgtc ttattatgat
    14161 atgaatggtg aaaaacacaa gattcgtttg aagaattatg aatcgatcgt cgttcaacat
    14221 gaaatcgacc atatcaacgg tgtgatgttc tatgaccaca tcaacgatca aaatccattt
    14281 gcactaaaag aaggcgtatt ggtgatcgag tagagaattg gcaggttcct tcgatactat
    14341 gctacaagaa agctttataa aaaaggggag tgctcaaaag ctgcacttcc cttttttatg
    14401 tttctccact aatcgaaatt tagatagaca tctagaaatc gcaattccaa aaaattctgt
    14461 gtattggata cgcatctttt cttatttttt cttatttctt ccatatgggt gtttatctca
    14521 gagatgatag aatagtttct aggataaaaa gtagcagcta cgatgttatc cttcgtagag
    14581 gattcctgct tcagctagtt tttgtttgat gtttttcagc atatgctttt ctgggtagcg
    14641 gatcgtatgc agatggatgc cgcctgttaa ttcggataac aaagtagcat tacttttttg
    14701 ataacgtctc ataaaggaat cgatatcttg tggcgttttg atgtgcaaag ttcctgctaa
    14761 ctcaccgtaa aggggatgtt ccacaattac atcaatgatt tcaccaccat tttcaacgat
    14821 tagtctcagt tcttcttctg tttgatcttt tccatgctgg acagcgattt tactgatgtg
    14881 cccagtattg gtttcttctg tttcgaggat ataacctctt gctgttgcca cgatactttt
    14941 acctgcagct cgtagtaaag caatatcgcc tacaatgatc tgacggctga ctccaaaagt
    15001 tttcgcaaac ttgctagcac tcactggttt ttctgtattt gccaaggttt gcatgatttg
    15061 ttttcgccgt gtctctcctt cgatcacact tattcctcct tcattttgtt ttggtattcg
    15121 cgcgagtgtt tcatcaaatc aacatacgta tctgttattg tttccttata tcccttattt
    15181 ttcagcagac taacaacttt atccaatact tgctgctctc tttttgcatc ataaactgct
    15241 tggttttctt cttttttcac ttgtcctatt ttagttaccg tatccattct tttttctaat
    15301 aaagcaatca actctcgatc gatcatatcg atcttatgac gtaatgtttc aagagactct
    15361 tctgttagtt cttcctctac tgtcttgatg atacgagcag gatttcctgc aatcactaca
    15421 ttgtcgggaa aagatttggt cacaaccgaa cctgcgccga ctactacgtt gtcccctaga
    15481 gtgacacccg gcgtgacgat aactcctccg cctaaccaaa cattattacc gattttaatg
    15541 ggttttgcgt actccagacc gctgttacgt ttcactggat gtagtggatg cgtcgccgtg
    15601 taaagctgga cattcggagc gaacatgcag ttatcaccga tttcaatcgt gctgacatct
    15661 aaaaatgtac aattgaaatt tgcataaaaa ttctccccaa caaatatatt atatccgtaa
    15721 tcaaaattga taactggctc catatagatt tttttccctg tacggccaaa agtctctttc
    15781 aacaattggg atcgcagttc cgctgattct gcttggttga tgatttggct ttgacttctt
    15841 gcaaacatac ggtcggcaac cagctcagga tcattggcaa aataaagctc gcctgcaatc
    15901 attttttctt tttcggtttt gaattcagtc ataatctgct cctaattctt gtattttttt
    15961 gaaaacaacc gctccgtctt cttgaataaa cgtataaggc tccgctaaat aaacaggcgt
    16021 ttgattgatt actaagattt tggctgacgg ctggcgaaac tgtaccagat cacaaaacgg
    16081 atgaacttga aaactcgttc caacaatgac gatcagctct gcttggctga cggcataagc
    16141 cgcctgctcc atgactccat cttgaaaccc ttcaccatat aacacgatgt ctgggcgtat
    16201 ttgtcctccg catgcttgat gtttgtcgct gtgaagatat tctttccagt ctaccgtttg
    16261 ttgacatttt cgacaatgac attgatacaa acttccatga aaattgacga gttgcgggct
    16321 tttagccttt gcgtgcaaac cgtcgatgtt ttgggaaaca acccaaacat ttttttcttt
    16381 cgctagatgt gccatttcca aatgaatgat attgggtttt gcttcaggat gataaatctt
    16441 tttgacaaat tgatagaatt tatccggttc attcaccatt gcttgatgac tcagcaagta
    16501 ttccggctgc tccatcccgt ggtagattcc tgacaaagat cgatagtctg gcactccaga
    16561 aggtgtggat acccctgcac cggtcaaaaa tgtaatttgc tgataagcta atatctcttt
    16621 tgctgcttcg tctactgtta attgtttcat gttttgtcac ctcactttcc attatagccc
    16681 atactcgaac aaatttattg ttgaaaagtc agaaaaatgg gaataaaaaa gtgttatggg
    16741 catttgaaca atttatcaca atgttcactg acaaaagaat gaaaacgtgt tacaatactg
    16801 ctgtataaaa gtgaggtgaa tcgatatgtt accattcttt atttattggg gcgttattct
    16861 ggcttgtatc gcctggttag cgttaagcgt ttatttctct gttttttatc ttgtacgcaa
    16921 agaaaatgga aatctttggg cttttgcttt attcaacgtg attgccgcaa ttgttttagc
    16981 cattacatta gccgtatatc gaacatgggg ctggggaatc acccaatact caagtttgat
    17041 ttatctaata ttagccattt atggcgtcgt tgttcttttg caagcgatcc taggacgtga
    17101 gccaaagaaa gcggcagctt aaatgatcga gatggaactg gacacaggaa attttctgtt
    17161 ctagttcttt ttttattgta ttacatacat ttttgtgtat aattatacta tcgtttgcaa
    17221 tggatagatg attcacattt tgattgtttt ttcggtaaaa gtaaagattt ctatatttat
    17281 cagtattttt tcaaaaaaca gcagttatag attgacaaag cctctcgagt atcgtaaact
    17341 ttttttgtat aaataactga atacgagatg cctcaatcaa tgaggtagag gtcgcgagaa
    17401 tcattaaccc tgtggagtga agcaaacatg gagaagcagg aatagggagg atcgccgaaa
    17461 tgtgcacttt tttgcttgga gtgtatgttg ggacgcaagt taagagcttg cggactgtct
    17521 tagcagtgat gctaagttgc gctatgtttt gtgagaggtt tatcacctta tggaatttta
    17581 gcggcctttt tatatctata aatcggtcgc tttttatttt ttcagataaa gctttaccgc
    17641 atttcatcta gaccgctcgt gtatcatata atcagaaagg gagaaaaaaa tgaaaaaaaa
    17701 tatggtttca gttgccgtac tatttgcttt tgtcttctca ctattcagtg tgggatctct
    17761 ggcaaatgca gatgaaaaaa caccgagcga tgagtttcgc gtaggtatgg aggctgggta
    17821 tgcacccttc aactggtctc aacaaacaga tgagaataat gccttgccaa ttcaaggcca
    17881 aaatagttac gccggcggtt atgatgttca aatcgctaaa aaagtagcag atggtttagg
    17941 gaaaaaatta gtcatcgtcc aaacgaaatg ggatggtctg gctcctgccc ttcaatcagg
    18001 caagatcgac gcgatcatcg ccggaatgag ccctactgct gaacgacgaa aagaaattga
    18061 ttttaccgat ccttattacg aatcacaatt agtagtagta gttcaaaaag atagcaaata
    18121 tgcaaaagca aaaagtttag cagatctttc tggagcaaag attactgctc agttaaatac
    18181 attccactat actgtgatcg atcagatacc agatgtgcag aaacaacaag caatggataa
    18241 tttctcagca atgagaacag ctttagcttc tggaatgatc gatggttatg tcagcgaacg
    18301 tccggaaggt gtaacggcaa ctagtgtgaa caaagacttg gaaatgcttg aattcagcgg
    18361 tgcagatggc ttccagacaa acccggaaga tgttcagatt tctgttggta tgcgtaaagg
    18421 cgacccggat atccaaaaag taaaccagat tctttctggc atctctaacg atgaacgaac
    18481 caaaattatg gaccaagcag tcaaagatca gcctgctgca actgatagtg aagaaaaacc
    18541 tggcatcatt tctgatttca ctacgatttg gaaacaatac ggcaatatgt tcttacgagg
    18601 tgctggactt accttgttca tcgcattgat cgggacagtt gtcggtacta cacttggatt
    18661 actgattggt gtcttccgtt cggtacctga atcaggaaat gcactgactc gcttcttcca
    18721 aaaaatcgga aatgcacttc tttctatcta tgtcgaagta ttccgcggaa caccgatgat
    18781 ggtacaagca atggtcatct tctacggtct ggcactcgct tttggcattt cattgaaccg
    18841 aacgattgcc gctttgttta ttgtctctct caatactgga gcttacatga cagagatcgt
    18901 tcgtgggggg atttttgcag tagatgaagg gcagttcgaa gctgcgcaag ccattggtat
    18961 gacccatggc caaacgatga gaaaagttgt tattccacaa gttttacgga atatcttgcc
    19021 agcaacagga aatgagtttg ttatcaatat caaagataca gcagttttaa gtgtaatcgg
    19081 cgtagctgat ttgttcttcc aaggaaactc tgcttccggt gcgaatttcc aattcttcca
    19141 aacatttacg atcgtcggga tcatctactt agtcatgacc tttacgatca ccagaatcct
    19201 gcgggcggta gaaaagaaaa tcgatggtcc ttcagcttat accaagcttg aagaaatcga
    19261 caattcaaat ttgcaggaat aaggaggaag aatcatgaat gaaaaaatta ttgaaatcaa
    19321 acatctaaga aaaagcttcg gtgaaaatga agtattgaaa gatatcagcg taacagtcaa
    19381 taaaggtgaa gttgtgacga tcatcggttc ttccggttct ggtaaatcta ctttcttgcg
    19441 ctgtatcaat ttactggaaa aaccaacagg cggcgagatc atttataatg gagaaaatgt
    19501 attagctcct aaatataatt tacctaaata tcgaacgaat ctaggtatgg ttttccaatc
    19561 cttcaacctg ttcaacaata tgaatgtctt ggaaaactgt atgtctggtc aaacaactgt
    19621 cctgaaacga gataaagaaa ctgccaaaaa agtagcaatc gaaaatctgg aaaaagttgg
    19681 tatggatcgt tatatcgaag ccaaaccagc acaattatct ggtggacaga aacagcgtgt
    19741 cgcaattgct cgggcattat caatgaatcc agatgtattg ctattcgacg aacctacttc
    19801 ggcgttagac cctgaaatgg ttggcgaagt gcttaatacg atcaaagact tagcacatac
    19861 tggcttgaca atgcttatcg tgacgcatga gatggaattt gcaaaagatg tatctgaccg
    19921 tgtcattttt atggataaag gtgtgattgc agaagaaggt acaccagaag atatctttgt
    19981 ccatccgaaa gaagaacgta caaaagaatt cctttcacgt attttaaatg cttaggatca
    20041 atagctgatt gggacaaaga gctgcggtgt ttaccgcagt tcttttttat gaattactca
    20101 taaaaggtaa atttctgaaa attttggaat cttagttgtg tttttcagaa aactctttta
    20161 atatggaagt aattaaactt ttttagttag gatgatttaa aaatgaatga agagcaagaa
    20221 ctgcagcgtg gactgaaaaa tagacatgtc caactgattt ctattggcgg agcgattgga
    20281 acggggttgt ttttaggttc cggaaagtct atccagctag ctggcccttc gatattgctc
    20341 gcctatctta ttactggatg catctgtttt ttcattatgc gtgcgttagg cgaacttctg
    20401 ttatcaaaca ccaataacca ttcttttctg gattttgttg cagaatatct tggcaaaaaa
    20461 gcggctttca tcactggctg gacgtattgg ttctgctggg tatcgattgc aatggccgat
    20521 ttaacagcca tcggaatgta tgtacgctac tgggcgcctg gtgttcctca gtggcttcct
    20581 gaactgattg cattaggtct tcttctttgc ttgaatatgg tggctgtctc attgtttggt
    20641 gagttagagt tttggtttgc attgatcaaa gttgtagcga tcattgcctt tatcctcgta
    20701 ggagcttata tgatcctcac ccactatacg acagatattg gcacagccag tatcacgaat
    20761 ctatggtctc atggcggatt ctttccaact ggtgcaaaag gatttatctt agctttccaa
    20821 atggtaacct ttgcttttgc tgggatagaa ttagttggat tagtcgctgg agaaacagaa
    20881 aatcctgaaa aagtgttgcc ggaagctatc aataatattc ctatcaggat catcttattt
    20941 tacttaggat ctctttttgt cattatggca atctatccat ggaacagctt gaatccagat
    21001 aacagtccat ttgtagaagt attttcggaa attgggatca cgattgctgc ttccttgatc
    21061 aatttagtcg tattaagtgc agcagcttct gcctgcaaca gtgcaatata tagtacagga
    21121 cgtatgcttc gttctttagc gcaagaaggc agtgccccta aaaaattcaa aaagttaaca
    21181 acacatcatg tgccagggaa tgctttgaca ttttctacta tcgtcatttt catttctgtt
    21241 atcttgaatt acgtgatgcc tagtgaagta ttcacactag taagcagtat tgccactact
    21301 tgtttcttgt ttatctggag tatgcttgtc tacacgcaca tgaaatatcg taaaagtatc
    21361 ctcggcaaaa aagctcattc atttaaaatg ccgttgtacc catttagtaa ttatctggta
    21421 tttctttaca tggcctttgt gtgtgtcgtc ttgttcctag gaaaagatac gagaatcgct
    21481 ttattactaa cacctgtgtg gttcttgctt ctattactaa tctatcatat gaaatatgaa
    21541 cgtaagaagt aagaaagtag ttgtgacaga agtggtcagc ttcgagaaat aagaaggaat
    21601 ttacgaaaat tgcttcttaa atttttgtga aatttcggct tatttcttgg agttaaaagc
    21661 ttctgtccca gcctcttttc tatttccccg gaaacaaccg ctgataatag gcatctgctt
    21721 ttttatagga aaaaatccaa ataccagata caaaaaaacg tccgaataac ccgatcaacg
    21781 gtacaaaaaa gacaccggca atcaagcata agttaatcat catggagata agacttcgac
    21841 ggtcctttga caaaactctc acgacatggg aatctggttt atttgctttc atgatttgcc
    21901 tcatcaagat accaaaagaa aaactccaca tcgtgtatac taagacataa gccatctcag
    21961 gccaaaaagc ttcagggtta cgcccgaccc aactagtaac agaaggacat aaagtcagcc
    22021 aaaagagcaa aaagatattc gaccagacca ctcggtaatt aattcgttct gccatctgaa
    22081 atatacgatg atggttcacc cagatcaccg caacaaatat aaagctgatg gtataagcta
    22141 ataatgtatt tcggatgacc cataaagaat gaaagctaga tgtattaggg atcttgatct
    22201 ctaatacaag aactctcaaa acaatggcta ccactccatc agtaaatgct tctaatctcg
    22261 actttgtcac gatgattcct cctcagatga ttcagcgctt ttattttatc acttacgtta
    22321 ttaaataacc gtcgttttat ataggaaatt ttctgaaaat tgttgcatat ttttcgaaat
    22381 tttgttactt ttaaaaaaac cgtaaaggag ttatgtatga caaaaaaatt atcatttaaa
    22441 gactatcttt ttataggctc catgcttttc ggtctatttt ttggtgctgg aaatcttatc
    22501 tttcccgttc atttgggtca agaagcaggc gctgctacct tttgggcgaa tctaggtttc
    22561 ttggttaccg gtatcggtct tcccttctta ggggtgattg ccattggtgt atcgaaaagc
    22621 tcaggtgtat atgaattagc aaaacggata aatcaaacat atgcgatggt ttttaccatc
    22681 ttgctttact tagttatcgg accgtttttt gctttacctc gccttgcaac cacatctttc
    22741 gagattggtt tagcaccatt tatcgatcct agcagacaga cgctatatct agcaattttc
    22801 agtattgtat tttttgtgct ggcttggtgg ttttcaagaa aaccgacaaa gatattagat
    22861 tatgttggaa agttcctgaa tcctgcattt ttggttttat taggtgcttt gcttctcctt
    22921 gcttttttga atccactagg ttctgtaaat caagcgccta ttcagccaaa ttatcaagag
    22981 catgcttttt tcacagggtt tactcaagga tataacacat tagatgcgct tgctgcttta
    23041 gcatttggga tcatcattgt cacaacaatc caaaacatgg gtgtaacaaa acctgctgaa
    23101 atcgctaaag acacgatcaa atctggtgcg atcagcatta tattgatggg aatcatttat
    23161 actttgctcg cttatttagg tgctatgagc ttaggtagct ttgctctaag tgaaaatggc
    23221 gggatcacgc tggctcagat tgctgatcat tatttaggta cttatggcag tatcttatta
    23281 gcatttatcg ttatattagc ttgcttgaag accggtattg gtttgattac agcttttgca
    23341 gaaacattca cagatttgtt tccgcaaaga aattacgcat tctttgttgc acttgctagt
    23401 atccttcctt gtttagcggc aaacgtaggc ttgacgaata tcattcaatt ttcgttgcct
    23461 gttttgatgt tcatctatcc attagcaatg acactgattt tactagtgtt agttggtcct
    23521 ttgtttaagc agcgtacggc tgtttatcga atgactactt attttacatt gattgcttcg
    23581 atttttgatg ggttgaacgc ttgcccagaa actatcaaac aaacacctat tgttcaaaat
    23641 cttttatatg ttgcggaaag ctacttgccg tttttcaaat tggggatggg gtggatcgta
    23701 ccagcagtta tcggttttgt tatcggactg atttggagtt tcgctaagaa agaagaagta
    23761 gcagattgat aaaagaggtt gtgaaagaga cgttcagcct tattgccgat aggctgtctc
    23821 ttgaacactg tttattgaga ggtcgtgaaa aagccgctca gtttcgagta ttaagaaata
    23881 ttttttgaaa atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc
    23941 ttttgaacac cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg
    24001 caaagtagct ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc
    24061 aaacaacatt tcttttataa ta
//
LOCUS       DAZKOW010000037        23830 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000037 DAZKOW010000000
VERSION     DAZKOW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.037"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(55..789)
                     /locus_tag="ACG912_001392"
     CDS             complement(55..789)
                     /locus_tag="ACG912_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFQ0512722.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACG912_001393"
     CDS             1116..1526
                     /locus_tag="ACG912_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFQ0512723.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACG912_001394"
     CDS             1519..2220
                     /locus_tag="ACG912_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFQ0512724.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACG912_001395"
     CDS             2220..3806
                     /locus_tag="ACG912_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512725.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACG912_001396"
     CDS             3825..4046
                     /locus_tag="ACG912_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFQ0512726.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACG912_001397"
     CDS             4043..4804
                     /locus_tag="ACG912_001397"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFQ0512727.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACG912_001398"
     CDS             5184..5693
                     /locus_tag="ACG912_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFQ0512728.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACG912_001399"
     CDS             complement(5768..6307)
                     /locus_tag="ACG912_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFQ0512729.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG912_001400"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACG912_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFQ0512730.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACG912_001401"
     CDS             7320..8171
                     /locus_tag="ACG912_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFQ0512731.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACG912_001402"
     CDS             8199..8834
                     /locus_tag="ACG912_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFQ0512732.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACG912_001403"
     CDS             8854..9627
                     /locus_tag="ACG912_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0512733.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACG912_001404"
     CDS             10035..10832
                     /locus_tag="ACG912_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFQ0512734.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACG912_001405"
     CDS             10810..11223
                     /locus_tag="ACG912_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFQ0512735.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACG912_001406"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACG912_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0512736.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACG912_001407"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACG912_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFQ0512737.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACG912_001408"
     CDS             16000..16560
                     /locus_tag="ACG912_001408"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFQ0512738.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG912_001409"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACG912_001409"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFQ0512739.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACG912_001410"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACG912_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFQ0512740.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACG912_001411"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACG912_001411"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFQ0512741.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG912_001412"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACG912_001412"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFQ0512742.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG912_001413"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACG912_001413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFQ0512743.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACG912_001414"
     CDS             21907..22485
                     /locus_tag="ACG912_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0512744.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACG912_001415"
     CDS             22537..23019
                     /locus_tag="ACG912_001415"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFQ0512745.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACG912_001416"
     CDS             23136..23510
                     /locus_tag="ACG912_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512746.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4286 c         5116 g         6645 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttatttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt
//
LOCUS       DAZKOW010000038        23742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000038 DAZKOW010000000
VERSION     DAZKOW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.038"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..807
                     /locus_tag="ACG912_001417"
     CDS             106..807
                     /locus_tag="ACG912_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFQ0512747.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1416..2432)
                     /gene="tsaD"
                     /locus_tag="ACG912_001418"
     CDS             complement(1416..2432)
                     /gene="tsaD"
                     /locus_tag="ACG912_001418"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFQ0512748.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2429..2893)
                     /gene="rimI"
                     /locus_tag="ACG912_001419"
     CDS             complement(2429..2893)
                     /gene="rimI"
                     /locus_tag="ACG912_001419"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0512749.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2900..3442)
                     /gene="rimI"
                     /locus_tag="ACG912_001420"
     CDS             complement(2900..3442)
                     /gene="rimI"
                     /locus_tag="ACG912_001420"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFQ0512750.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3426..4148)
                     /gene="tsaB"
                     /locus_tag="ACG912_001421"
     CDS             complement(3426..4148)
                     /gene="tsaB"
                     /locus_tag="ACG912_001421"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFQ0512751.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4339..5319)
                     /locus_tag="ACG912_001422"
     CDS             complement(4339..5319)
                     /locus_tag="ACG912_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0512752.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5343..6827)
                     /gene="galT"
                     /locus_tag="ACG912_001423"
     CDS             complement(5343..6827)
                     /gene="galT"
                     /locus_tag="ACG912_001423"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFQ0512753.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6839..7828)
                     /gene="galE"
                     /locus_tag="ACG912_001424"
     CDS             complement(6839..7828)
                     /gene="galE"
                     /locus_tag="ACG912_001424"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFQ0512754.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8076..8243
                     /locus_tag="ACG912_001425"
     CDS             8076..8243
                     /locus_tag="ACG912_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFQ0512755.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8305..10116)
                     /locus_tag="ACG912_001426"
     CDS             complement(8305..10116)
                     /locus_tag="ACG912_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFQ0512756.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10113..10478)
                     /locus_tag="ACG912_001427"
     CDS             complement(10113..10478)
                     /locus_tag="ACG912_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFQ0512757.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10641..11036)
                     /locus_tag="ACG912_001428"
     CDS             complement(10641..11036)
                     /locus_tag="ACG912_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFQ0512758.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11054..12016)
                     /locus_tag="ACG912_001429"
     CDS             complement(11054..12016)
                     /locus_tag="ACG912_001429"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFQ0512759.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12016..12228)
                     /locus_tag="ACG912_001430"
     CDS             complement(12016..12228)
                     /locus_tag="ACG912_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFQ0512760.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12249..12947)
                     /locus_tag="ACG912_001431"
     CDS             complement(12249..12947)
                     /locus_tag="ACG912_001431"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFQ0512761.1"
                     /translation="MNYQQQIKLRVWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12967..13509)
                     /locus_tag="ACG912_001432"
     CDS             complement(12967..13509)
                     /locus_tag="ACG912_001432"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFQ0512762.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13641..14645)
                     /locus_tag="ACG912_001433"
     CDS             complement(13641..14645)
                     /locus_tag="ACG912_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0512763.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14642..15631)
                     /locus_tag="ACG912_001434"
     CDS             complement(14642..15631)
                     /locus_tag="ACG912_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFQ0512764.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15628..16434)
                     /locus_tag="ACG912_001435"
     CDS             complement(15628..16434)
                     /locus_tag="ACG912_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512765.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16600..17556
                     /locus_tag="ACG912_001436"
     CDS             16600..17556
                     /locus_tag="ACG912_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0512766.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17633..18151)
                     /locus_tag="ACG912_001437"
     CDS             complement(17633..18151)
                     /locus_tag="ACG912_001437"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFQ0512767.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18239..18388)
                     /gene="rpmG"
                     /locus_tag="ACG912_001438"
     CDS             complement(18239..18388)
                     /gene="rpmG"
                     /locus_tag="ACG912_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0512768.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18616..19062
                     /locus_tag="ACG912_001439"
     CDS             18616..19062
                     /locus_tag="ACG912_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0512769.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19257..21152)
                     /gene="asnB"
                     /locus_tag="ACG912_001440"
     CDS             complement(19257..21152)
                     /gene="asnB"
                     /locus_tag="ACG912_001440"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFQ0512770.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(21477..22451)
                     /gene="bsh"
                     /locus_tag="ACG912_001441"
     CDS             complement(21477..22451)
                     /gene="bsh"
                     /locus_tag="ACG912_001441"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0512771.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(22785..22872)
                     /locus_tag="ACG912_001442"
     tRNA            complement(22785..22872)
                     /locus_tag="ACG912_001442"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22838..22840),aa:Ser,seq:cga)
     gene            complement(23017..23583)
                     /locus_tag="ACG912_001443"
     CDS             complement(23017..23583)
                     /locus_tag="ACG912_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0512772.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         7162 a         4995 c         4042 g         7543 t
ORIGIN      
        1 tgtaaagtat taaaacttgt ttctaatgtt ggtatgttaa aactttacaa attctttaaa
       61 aaaagtgaca ataaatatag tgaacaataa ggagaggact ttttaatgcg tgttaataaa
      121 atcgattttc acttttcaaa actcagatat caacctgatt ttgaacattt ctcggatgaa
      181 gagttccaac agatcaagga acatactgtt cttcgttctt ataaaaaagg acagatcctt
      241 ttcgacgatg gtgatcccag acaacggttg tatatcttga ccaaaggact cgttcgttta
      301 gaacgttatg atgaaagtgc aacttattac tactatgact atgtgaatac aaataacctt
      361 tttcctatgt caggaatgtt tggagaagag acctacactt atacagctca agcgatgact
      421 gatattgaaa cattctacct tcctgtttcc tactatgaaa aattagcacg aaaaaatccc
      481 gaacagttga tctatatgat acaaaaaatt tctaaagtag ttgaaataca tgaattgcgt
      541 atccaaatcg gattgacctc tagcgccttt gaccgggtaa aacaaaattt attcattttg
      601 aaagaagaat tgggcgtacc aacaaaagat ggtcatatca ccattcctta tccgatcacc
      661 ttaaaagaat tagcagtgaa tagcggcacg acacgtgaaa ctgctggaca agtaatcaaa
      721 caactacgtg attccgggct gttagattat gacaaaaagc gttttacttt ctgtaagaca
      781 gctactactc cagctatgag agaataacgc gaacaaaaat aagcagtgac cctgataaat
      841 atcagggtca ctgcttattt tcctccctaa aacttctgaa agatccggca gttttagatc
      901 actggacaag actcaaagat tttctatgtg tgtttcagtt atgtaatatt cactgtttgc
      961 aaaaaatact gttagattaa ctagatgaag cgtcttgtcc tcatccgatc tagattattc
     1021 gatattcctc gacaataaac gattactcag tgagggcaat cagaggaacg gataatgata
     1081 aataagctgt aaaaaatagc aaaataacaa taatgattaa aagtatgtaa aaaatagggc
     1141 tttgacaaaa acgtgtcaca gccctatttc gattaaacgg tgtgtacctg tcaactcttc
     1201 ggtcacaagt cataatgttc ataaacatga ggagctgttt tttgaaattg ttccttgtga
     1261 atcagagtta aacgacaggc ttacgaactc ttgattaaac ggcgttcaaa agcttgcata
     1321 cgcggtaata agaacagcca ggtcaaaaac atgagaagcc gtttttcctg ggctgttctt
     1381 attacttgat gcagggcagc tttttcacaa cctctttatt cagataaaac caagcttgga
     1441 tcagcattca actgatagct agcaaaattt cttttttgca tttccacatg tgctgctgct
     1501 ccaatcattg cagcattatc tccacaaagt cgaagagggg gaatcactag ttctacctca
     1561 gggagtttag ttgccaaagc atcctgcaat ccttcacgca acccttgatt ggccgctaca
     1621 cctccagcta taatcagttg cttgactgga taattttgac aagctcgaag tgttttagta
     1681 atcaatacat cgatcacact tgcttgaaag ctagctgcta aatcattttt gtctagatct
     1741 tctccacgct gttgtgcatt atgcaccaaa ttgataaatg cactttttaa tccgctaaaa
     1801 ctgaaatcat aattatcttc atggatcatc gctcgcggaa aatggtaatt atcttttccc
     1861 tgatgagcta gctgatcgat ttcttttccg cttgggtaag aaagacctaa tactcttcca
     1921 actttgtcat aagcttctcc tgctgcgtca tctcttgtct ccccaatgat ttcatatgat
     1981 ccatcttctt gcatatagac taattctgta tgtcctccgc taacaagaag tgccatcaat
     2041 ggaaactgga atggtttaac cagacgggct gcatagatat gccctgccat atggtttaca
     2101 ggaataagcg gcaattggtg tgcccaagca aaagccttag ctgcagaaat accaatcagc
     2161 aacgagccga caagtcctgg tccataagtg acagcgactg ccgataaatc ttctgccgac
     2221 acaccagctt ctaccagtgc atcttccaag cataaggtga tttgttctac gtgatggcgg
     2281 ctagctactt caggtactac gccaccaaaa cgtttatgac tgtttatttg agaagcaacg
     2341 atgttagata agatctctgt tccatttcta acgaccgcta cactcgtttc atcacaactt
     2401 gattcaatcg ccagaatcaa ttcttcattc atttcatcgt ctccgttttt aactttctgc
     2461 tcataatgat cgcatcttcc gtcggattgc gataatatga tttccgttta cctaatatac
     2521 gaaacttttc tgattcataa agttttcttg ctgcttcatt agatagacgt acttctaaaa
     2581 aaacatgcag cgaatctttt atttgttccg taatcagtaa ttgtaacaaa tgccgtgcat
     2641 gcccttttcc ttgttctgaa actgcaacag caatatttgt gatctcgact tcatctatta
     2701 ctttcgagta tccaataaag ccacagatct ggttatcttc agctaaaagc agataatcag
     2761 agtgaggctg ttggatgtct gccaaaaact gcattttcgt ccaaggtgaa ccatgtggat
     2821 aagcattttc gctaatcatc catacgcatt ctgccaagta cgcttcatca aaatcttttt
     2881 ttctgcaaat caactaatct cactcatttt ctaaaagttt tatcatatcc aacgcatctt
     2941 cttttgtttc agtgtaatat ctttttttga tcgttgttgc ttgaaacccc aatttccgat
     3001 aaagtctttg tgcatcttga ttactcatac gcacttctaa tgacatcgtc tcacagcgat
     3061 tcattatagc aaagttctcg atttcgtcaa taagcagcga accgattcgt ttacgctgat
     3121 atgccggtcg aacggcaata ttcgtgatat ggcaatctaa accgaacgct cttgaaccga
     3181 taaaaccaat cattcttcca ttatcaaaga tacctagata tagatgagga agcggtgact
     3241 tcaattctga tataaaggca ctttttgtcc atggcaattc gccattatag acttctcgtt
     3301 cgacatccag cagctctttg atatcttctg tagtcaattc tcttagtacg taacgacgat
     3361 cgatttgttc gactgttttt tctgtataaa tcattttatc ttgaaaaaag cttctggcat
     3421 acgctctaaa ttttttcaac ataactttcg tctccaggtg tgtgcgtctc caaccatttt
     3481 tcttctgctt ctactcgttt taaatagcga ggaagaaaag cttgtttctc tgcaactggt
     3541 tcaactaaag cacctaattg agccacagtt acaccacttg gaatatccca tgctggaact
     3601 gtattaatca tcgcactagg caatatttcc tttatttcag agaagaattt gtgacaatca
     3661 ctcccgacga aataaatagc ttcattcagc ttttgtagtt gtgtgaacaa atcagaagca
     3721 gaaatatgta catcttcaag tacagtttct aatctatttt ctttccagcg gtatgcccca
     3781 gcgtatacat tttttcttcg agcatcaaac atgggcacaa taatattatc cacaccgatg
     3841 caattagccg cgatggtttt cagactagat actcctacta gctctttatc taatgtataa
     3901 gcaatcgttt ttgctgtcgt tacacctata cgaagccctg tataagaacc gggaccatca
     3961 gaaaccgcga ttcggtcaat gtcttttgga cttatttgaa gatctgcaaa caattggtcg
     4021 attgcaggca tcagtgttac actgtggttg cgtttgatat ttgtttgtat ttgtccaaga
     4081 atttgttcgt cttctaccac accgatcgcc aaagtttggt ttgcagtatc tattcctaat
     4141 gtaatcatat atttgatcct ttctgctatg acattctcgc cttccattgt agcatacttt
     4201 tttattctga ggatgaaaat accaaaggca aaactagtac aataatacat gattatctgc
     4261 tggcgggcaa attttgcccc ttcctgaaac acaaaaaagc tgcggtatca ctccattttc
     4321 ccacagcttt tccatctttt atttcgttga ttctcgaata atcaactctg tttccacaat
     4381 aatctttctt gaaaccggtg ctttggaatt acttaattga agaatcgtct ccactgctag
     4441 ctctcccatc cattcggtat aaacttttac agtagaaagc gagggcgtta catatttcgc
     4501 tacactgaca tcattgaagc ccatcacaga aatatcttct ggtaccacta aacctttttc
     4561 ttgaatcgct ttcagtgcgc caatcgctaa tgcatcatta gctgcaaaaa gcgcatcagg
     4621 aacatgcgga ttcgttttta aatactgctt cactgcttga tatcctgact cgacagaaaa
     4681 atctgcttcg ataattggac cttcgtcgta gcctttttct gccaaaactt gttgaaatgc
     4741 cataattctg ggatctttta gccgctgatg gtttttcttc gtatattctg ttccagcaag
     4801 catagcgata tgttgcgctt tatgggagac aaagtgttca atgacttttt gaacacttga
     4861 cttaaagtcg acaactagag agtcgattcc ttgagaagtg ccgtcagaat ctaccaaaag
     4921 caaggcattt tgggatcctt tcatttctaa taactgagct tcgtcgaatt ttcctagtgc
     4981 gatcgttcca tctgccggaa gatcactaat ggaatcccac gtttctttca ataaagtaac
     5041 gtttgcttct tctgcctttt tttcgattcc taggcgaata gataaataat aaagatcttc
     5101 aagctcttcc gtatcgtcgt accactggat caggcgaagc gtttgcttag tatttgctgc
     5161 atttttacga tgtttcgtat aatttaagcg ttctgctgct tcaaaaattt tttgctttgt
     5221 ggctaagcca acggaaagat caggatcata gttgagaact cttgaaacag tagcaggaga
     5281 aactcctgcc aactgcgcaa tgtctttaat tgttgccatt tgtctctcct ccttttttac
     5341 gtttataaaa tcgcagtaaa tcgttcaaac gctgcctgtc ctttttcatc tcgcttgaat
     5401 actccagcgt cttccagtac acgtgcaaaa acctgaccta cagatttttg tacgacatcg
     5461 gtgaccgtat ctttcgtcaa atcaggatgt tcttttttta attgttctgc ccacggacga
     5521 tgatattcag ccatctggtt ttcttctcct aataagaaat gctctacttc tactagttct
     5581 ggtttcaaac gaggtggtaa aacagcaagc cccattactt cgatcaaccc aatgttctcc
     5641 tttttgatat gttggacatc tttatgtgga tgaaaaatcc catcaggatg ttcttctgaa
     5701 acattgttgt ctcgaaggac aaggtccaac tcaaagtaat tgtcccttct tcgtgcaatt
     5761 ggcgtaatcg tatggtgtgg tgtaccatct gctgaaaaag ctctgataga tactgtttca
     5821 tccgaatatg ctttccattt ttccaatact gagttagctg cttcaataag caagtttttc
     5881 tctgttgatt gcaaacggat cacagacatc ggccatttca cgactcctgc gatgacttgc
     5941 ggataatctt ctagtttgat aagtttttct atcggtgctt ttgccatagg aaattcatga
     6001 cgtcctgcct gataatgatc atgagaaaga atcgatcctc caacaattgg taagtcagca
     6061 ttcgaaccaa caaagtaatg aggcaagact tcagtaatcc gaagcagtct agcaaatgtt
     6121 tcctttgtga ttttcatcgg gcggtgttgt tctgataaaa tgatcgaatg ttcattgtaa
     6181 taagcataag gggaatattg gaatccccag ctttcaccgt ctagattcat tcgaataatc
     6241 cggtgattgg tgcgggctgg atagttcaag cgacctctat atccttcgtt ttccatgcaa
     6301 agcatgcagg taggatagtc tacttttacc gcctcgcgtt ctgctgcgat ttgttttgga
     6361 tctttttctg gcttagataa attgattgtg atttccagtt caccatactc cgactctgcc
     6421 ggaaatttga tgttttttgc gatggctctt gttttgatgt agtcattttt cttacttaaa
     6481 ttaaagaaat actcagttgc ttcctctggg cttttcgaat agtgctgtgc aaagaatgca
     6541 ttgacgactg aaggtggcgg tgtgagaaaa tccattaatt gcgcttcaaa aatctcttgt
     6601 tctgctggca attctgaaag aacaccattt tctcttgcct tagcgacaag ctgatccaat
     6661 aagtctacag attcttcctt aactggtctt acttcaatgc tttctagagc atcttctcct
     6721 accatcccta aaagacgatt ctgtaaatat aagcggtcca tttccatcca tccgccagct
     6781 tcgatcgcaa gtgtggcaaa atcacaaatc gtttgactga ttgacatatc tcattcccct
     6841 attcttcata accatttgga tggctgacat gccaatccca tgctgttttg ataatcgctt
     6901 tgatatctgt gtattcaggt ttccagccca gaatctctct cgctttttca cttgaagcga
     6961 ctaatcggct aggatctcca gctcttcttg gtgctatttc tgctggaatt tcttttcctg
     7021 tcacttctct agctgcttct agcatttcct tgactgagta tcctttgctg ctgcctaaat
     7081 tgaagaagtt gctttcattt ccttctttca agtattccaa agctaaaata tgtgcagcaa
     7141 tcaaatcttc tacttgaaca taatctctga tacatgttcc atctggtgta tcataatcgt
     7201 ctccataaac agccaaggct tttctttgac caagagctac ttgtaaaatg attgggacaa
     7261 gatgcgtttc tggtgtatga tcttctccga tagatgcatc tgcttttgct ccggctacat
     7321 tgaagtagcg caaagcgaca tagcgcatgc catatgcttg gtcacaccac ttcatcattt
     7381 tttccatcat caatttactt tcaccatacg gatttttagg attagctggt gtatcttctg
     7441 tgattggaga ctctttcggc tctccataag ttgctgcagt agaagagaat acgattttat
     7501 tcacgttgaa ctcgtgcatg acttcaagta agatttgcat accgtatacg ttgttgttga
     7561 aatacatcaa tggtttttcg acagattctc ctactaaaga gcttgcagca aaatggatta
     7621 ctccttcaat cggttccttt tcaaatacgg aacgtagaaa ttctttatcc cggatgtctc
     7681 cttcataaaa atgagcatct ggatgtactg cttgtttgtg acctgtgagc aaattatcta
     7741 ccacagctac ttgatacccc ttttgaacta gctggtctac agcgtgcgaa ccgatataac
     7801 cggctccgcc tagtactaaa attgtcataa aaaacactcc tttgattagt aaaattgtgt
     7861 ttgttgattc tatataattt aaaaaacgaa gtttactttt atggtgaaaa caaacaaagt
     7921 aaaatttcgt aaattcatat ggtaaattta taataaaaca tttagtaaac attttcaagg
     7981 attttttgca aaggtttaca aacaaacgta aaactagcct ttttttctct tttgttccat
     8041 aatagatata acaaataata aatgaggtgg attcaatgaa aaaatttgta tctggaatat
     8101 tagtaggaag tctggcaaca gccgctgccg ttgctggttt ggttgcttct gtgaaaaaaa
     8161 cagtgatcga tccaattgac gaaaaagaag caatgatcga agaaaaccgt aaaaaagcaa
     8221 tgcgtaaacg cgtttctcgt taataattat ttaaaaatac gcatgaagac tttctaaaaa
     8281 acagataagt catcatgcgt atttttattt gtcagtcaat ataagttgtg ctattttatc
     8341 tgcctgtctc agattccatg gatctgtata aggtctagct actgctgcag ttagccaatc
     8401 cacaccttca acaggtattc catagcaata aaagtcagac tggatttccc ctgaagcatc
     8461 aatcacgcga ttcgtacctc gctcaacatc tatagcttct gaatgataac ttcctgtatt
     8521 atctgaaaat gaaaaagggc gaacaagttt gttttccttc aaatttttta tgacaggatt
     8581 acttgaacga gaaaagcttg tttgtggcaa tcgagcttca atcaaatagc gactgctgat
     8641 ttctttatct tgtagagaaa taacatagcg gttgtgcttt tgctgaatat caaatggcgg
     8701 ttcgatcaaa tgaaaaatac ccgcatctat cagtgctgct aattctttcg tccgaatgat
     8761 tggcggaccg atcgttaaaa agctatttag atgagtaaac catccccata gtatttcttt
     8821 gtattcctta gcagaaaaaa cgtgccattc aatagcctgc cggattggat cacgccagtc
     8881 ttttaatgca tcaaatgttg aagcaaatgc tcctgtgcaa ttgccttttt ccgcttcttt
     8941 tatctgccct tcaataaata ttcggctagc ttctgaaaag ggctcattct gataggcaag
     9001 aggagtttct aaatacgacc aatcccatct ataagggatt agtgctggat gctttccgag
     9061 acactcctct gaagagaggg agagaaaatc atgctcaaaa tttttctttg gaatcggcaa
     9121 gtgatattct tcgatgattt tcttgtaata aaacaactca acgtcttttt tcagatagtc
     9181 aaaaaatgtt tcgccagtca aaaatcccga ctgatgccat tgctccaggt tttcatttgt
     9241 tagtaaccgt ggccatgcca ttgctccgcc ttgcttttga tttcttcctc gagggtaata
     9301 agggagcccc cttcttgatc cactgtatac aatcggttcc ttgcctgatg gatgatagac
     9361 cagttttccc ttttcgttct cgaagcgccc tcctcttgat actgtaaaca atccgacata
     9421 atcaaaaaaa cttaagccta gacctcttaa aaagacattt tctttaggcg gtatcttcga
     9481 aagatctaca tctgcaggat tagaaggaag ctgatacagt aaaacatttt tctttgcgta
     9541 gtctacccac actctttctt cttctgtctc atcattttcc cagtgaccgc ttgctaaaac
     9601 aagctgatcg atagaatagc tttgctgatc tgtctgtaaa agataaccat cttttctttt
     9661 gcttactttt tttactaaag aataaacaaa ctgaatagaa gctggaaatt cttcttgcaa
     9721 ctttgagaaa aaccagcgtt gatagagtcc gtataaacat cgagtagatt gctgatcttt
     9781 ttccagacga ttgatttctt ctataattag ttgcttattt ttggttaccg tctgttcgat
     9841 ataatctgta gcaggtcctt tactccattg aaacaagttg ggtccctgtg ttaccaatcc
     9901 tccactggac aatgtttcat ctgtaaaaag cgtcacttgt tgagaaacag aattcattaa
     9961 taactccggt ggctgattca accgccaaac tcgacctcca ggaccattgg gatcgaaaag
    10021 aaaaagttca agagaatgtc tgtcctgatt attatggaga atcctttctg ctacacttaa
    10081 tcctctaggt ccggcaccaa tgatccctat tttcatttgc atgcctccat ttcgataaat
    10141 tttttcaata aaacagcatg gttctgatgt tcattttttg caccataaac aaaagtaatg
    10201 actggatgtt ctttcataag ttgactgagt ttaaaaatag cttgcttctt ttcttcttcg
    10261 aaaatcaatt cttgctggta tttctttgca aatgaagcaa atcgttctgg tacatggtca
    10321 aaagttttgc gtgcttcatt actaggtgca atttctttca accacagatc taactgcgct
    10381 ttttcttttg aaattcctcg tggccagaat ctatccacga gtattctata gccatcctct
    10441 ttttcagcat caaggtaaac acgttttaat ttgatcatag ttgtttcctt ctttctacct
    10501 tttcgcttag cttatatatg tagataatag cacaaaataa aaaaaatgaa cggcacaact
    10561 atccattgcg ccgttcatgt gatcttggtc aaaatctaat gattgattcg gtttttaaag
    10621 ctgtcataca atcatctgtt ttatactttt ttacttttga tttttccaga ccatccgtca
    10681 tatccaccct taagtaaata aatctcttga taacctgctt tacgtaattt atttgctgta
    10741 cggatgctca aacttttctt ttgatcgtaa atatagacag gctggtcttt tcgaatagat
    10801 cctaatgaat ctttcaaaac agtgtaaggg atgtttcttg cacctaaaat atgcccagca
    10861 tcaaacacat ctttttcgcg aacatcgatt acttgtgctt ttctcattcc ttctcgaaat
    10921 tcttcttctg tcaaagtcgt tgctgaacgt ttggccatta ctcgtagata gacttcccaa
    10981 aggatgattg caagcacaat caatagcaaa atgatattaa ttacccataa aaccatgtaa
    11041 tgcctccgtt tttttaagca aatttcaagg ctaatgaggc agcaccaatg acaccagctt
    11101 cattaccaag ctctgctaat ttgatttttg ttgattctct tacttgcggg aaagtaaatt
    11161 ctttaaaata tttctctaca cggctacgta aaaattctcc tgctgccgat actcctccac
    11221 ctaacacgat acttgacgga tttaatgtat tccccaagtt tccgcaagct aaacctaagt
    11281 agaagcatac gcgatcaaca accatcaatg caaacggatc gtcttcttga gccatttcaa
    11341 agatatcttt acttgttact tcttctccgt tgtctagcat tgctttcagt tttgaatccc
    11401 ctgcgtattc ttctgccaag aaacgaccta ggcgtaccac acctgtagcg ctagatacag
    11461 tttctaaaca tccgcgttta ccgcaagtac attcgaaccc ttcaggatcc acagtgatat
    11521 gtccgatttc tcctccagct ccgctcacgc cgtgaatcaa atttccgcca gcaacgatac
    11581 ctccgccaac acctgttcct agagtaacga atacaacgtc tggatcgttt tctccagcac
    11641 ctttccatcg ttcacctaaa gcagcaacat tcgcgtcatt atcgatagcg aatttgattc
    11701 ctgttcctgc ctcgatcagt tttttcacaa attgaacttc tgtccagttt aagttatacg
    11761 caccaataac cgttccagct tcgctgtcca cgcttcctgg ggtacccatg ccgattccga
    11821 taaagtcttc ggctttcata ttatataaat tcaaacgatg attgattgat tcaatgattt
    11881 caggaacgat atgcttacca tcatccaaaa tattggtatc gatgctccat ttttgctgaa
    11941 tctctccttc tggtgttaaa attgcaaatt ttgctgttgt tccacctaaa tcaatcccaa
    12001 taatcttctt gtccattaca aatttccttt ctttcggctt tcttcaattc ggtgctctct
    12061 ttttaatatg cgccacgctg tcatgtatgt atcatgatca atcaaacggc tatcatacag
    12121 attacgcaat tcgatagtca ttaattcaat gtcatagatg cgtgcaccca catagataaa
    12181 gatgccaaat tttttgaaca gctgctgtac atcatataag ctctccatct ttaagcactt
    12241 ccttcatatt atacaagtaa accatatttt tttaaaccca gtaaaataca tattacaaaa
    12301 agaaatacaa aaactagtgt agaaagaatc ctttggtgta tcgaatagct tgtctgttgt
    12361 ttcggtagac ccaaaacatt ccctaacact aatccgccaa ttagcccacc gacatgtccc
    12421 caaacatcta cagaggtatc cagaagacca aaaacaaatg tcaacacaat aaataaaaga
    12481 aaatgtctga ctagctgctg gatcgctggg ttatgtttaa aatgaaaccc taagataaac
    12541 aacgcaccga acaaaccgaa tatcgaagta cttgcaccac cagacagaac attcgcttcg
    12601 ttaaaagcaa agcttgctgt atttcccatt atcccgctga acatataaat caagaaaaaa
    12661 cgccaatggc catagattgc ctcgatttgc tgccccataa aatacagtat cagtgagttc
    12721 attacgaaat gcatcagtcc aaaatggatg aagattggtg tcactaaacg ccaccattca
    12781 ttgaagtgaa caagataagg accatacatt ccggcataat aagggatctc aagtcgcgga
    12841 aataattcca tcatgaggaa gaccgctgtc tgtatcccta aaaaagcata ggtccataac
    12901 ggtcgtctta accatacacg caattttatt tgctgttgat aattcatgaa aacctcccat
    12961 ttcattttag tcaataaaca attgttggat cttctgatca aagctctccg gttgccattg
    13021 ttcattcaat tgttcacgaa aaacaaggct acatgttttt ttatgaaaat tatttaaata
    13081 ccggtcgtaa taaccaccgc caaaaccgat tcgataaccg gctctattgt agataatacc
    13141 tggtacaatg aggagatcga tcatctccgg caaatagagc ttctctgatt gtggctcaag
    13201 aacaccaaaa ctcgaacgta caagaacgga ctgttcatcc atttcataaa acgccatctg
    13261 acgtttcggc aaagttttag gtacaactat tttcttttgt tctttttctg ctcgttggat
    13321 aaccggctgt gtatccagtt caaattcatt cgataaagtc gtccctatca cttttgctga
    13381 cttccagtac tcgctggcga atagctgttt atagattcgc tgttcttttt cctttttctt
    13441 gccacttttg gccagatttt tcagcttttc gattcctttc cggcgtaatc ttactttcaa
    13501 ttggtccaaa aactcaccct tccttctctc ataaaatggt ataggaaaaa aagaaaaata
    13561 gcaatcgaac tttcaaataa atgcataaat agaataaaaa gaagacctag aaatgattgg
    13621 ccttcctttt ttaatgaatt tcaactgttc agcgcgtgtt tttgtctgat aaaaagtgca
    13681 tacagaaaat atggcgcacc aaaaagggca atcaaaattc ctgtaggtac ttccccactt
    13741 agtagaatca ctctagcaac catatctgca ctaagcagaa aaatggaacc gaacaaaccg
    13801 ctcagaacaa aggtccagtt attttcttta cgtacgacta gcttcgcagc atgaggagct
    13861 agtagtccta caaaagataa actacctgtc accgctacac taccgcttgc caaagcaaca
    13921 gctaacaaca aaaggatttt ttgactttta gccacgttaa ggcctaatcc ttgcgcgcgt
    13981 tcttgaccaa atgatagcaa tacaatcctc tgtgcataaa gtcctcctag cggaattaag
    14041 ataaccgccc atggaagcaa tgcccaaaca taaggccagc tcgcacccca gattgatccc
    14101 gctaaccatg cattcacaaa tccataattt tccttagata attggatcgt gcatagcaat
    14161 gttaatgcag aaaggccggc attaacggca ataccggaca ataagattcg attcataccc
    14221 aaaaagccat ttcgatcata ggccgcaaga tagacacagc aagcagctaa aacaccacct
    14281 agaaaggcta caactagcaa tagaaacggc agcgaatagt gcgaaaagaa acctaaataa
    14341 agcatcacta aaaatcctgc tccggcatta atgcccaata tactagaatc tgccaattca
    14401 tttcttgtta ctccttgcaa gatataacca gaaacgccaa gacaagctcc tgctaataaa
    14461 ctgatcagta atcgaggaac tcgaaattct ctaacaatca gcgccaaaga agatgattct
    14521 ttccctatga acgtggcaaa aagctgatcc aggtaaataa aaggcgtacc aatcattaaa
    14581 ctacctatag acaggatgac aagtacaaaa agcaagaaat agaacgtcca atatattttt
    14641 ttcaccgttg ttccctcctt acttgtatca gtaaaaacgg aacgccaatc aatgcaatga
    14701 ctaatccaaa aggtgtttca aaagggggat tgatcatcct cgctgtaaaa tccgccaaca
    14761 taaccaatcc accgccaact aaaaaagaga caggtagaac ataacgatag tccgttccaa
    14821 ccattccgcg aacaactggc ggaaccaaca gcccaataaa tgaaacaggt ccgactaacg
    14881 aaacggcaat ggctgctaac actgcaacta gacagaaaac aatcaaccgc gttcgtttag
    14941 gatgacttcc aagagctaat gcatgtgttt cactcaaatg aatcaaagat attgcttttc
    15001 ctaatgaaaa agaaaccaaa acagcaagta tatataaagg aaacaatact gctaattgtt
    15061 tccatgtgat attggcagct ccgcctacaa accaaaaagc aagatcctgc tgcagattaa
    15121 agaattgggt aagtgcttgg ctgatagcag taaaaaaaga actaatagct acgccagcta
    15181 aaatcagctg taaaggattc atgcccattt tcgaaaactg caaaaaacca aatacgagca
    15241 aagttacagc aaaagaacct gccaatgaca ttagaaacac agaaaaatca gaagctttag
    15301 gccaaaaagc aaaactaagg gccattgcca acgaagcacc cgcgttgatt cctagtaagc
    15361 ctgaatctgc taaaggattt cttgtcactc cttgcatcaa tgcaccgctc aatgcaaaac
    15421 aactccctac aagaaatgct cccgccacac gaggcaaacg aatatttcgt atgatttggt
    15481 gactttgttt tgatctatca aaatcaaaca acgcctgcca aaaatctgct tttcccacag
    15541 tcgctgcgcc atttgaaaga gcaacaaaaa aaataatcaa aagaacaaaa cagaccaaaa
    15601 gcaggagtct atttttcttc atagtcctca taattgatcc tcctgctctt ttggcaacaa
    15661 atcataggtt agaaaaactg gtttatttgt tcttggaagc gtgacaatcg ttgcatcaat
    15721 ctcaaatatt tttttcatac actcttttgt gatcacttct tcaggtgtac cttctgtgat
    15781 caacttaccg tttttcattg ctaataaata atctgaaaaa cgtgaagcat gattaatgtc
    15841 atgaatcgac atcaagatcg ttgtttgttt catttgattg atttcagtca ataactgtag
    15901 gatctctaat tggtgtgccg gatctaaaaa tgttgtcggt tcatccaaga tcaagatatc
    15961 tgtttcctga gcaagcgcca tagcgatcca gacacgctgg gtttgtccac cagacagcgt
    16021 tgcgatatcc cgcatacgaa atccagctag acctgtagca tccaacgccc actcgattgc
    16081 ttcatgatct tgcgtgtcca tgccactgaa cagtttttga taagggtatc gtccgtaaga
    16141 tactacttct tctactgtca tacctgtagg gtgttcacta gtttgtgcta ataatgcaat
    16201 tttttttgca agttcttttg aactgtatgt agagatatca gagttattga caatgatctt
    16261 tcctgatttc acaggtagta tacgggtaaa acttttcaat aaagtagatt ttccacttcc
    16321 atttggccca atcaaactag tgattttccc ttcaggaatc gtaacagaca tgttttcaat
    16381 gatttttttg ttttcgtagc cggctgcaat gtcagctgct cttaatctcg tcatttcacc
    16441 catcctttcg ctgaaaatca ttctcaatta tgacgctgtt tcaacatttc gtcaagcgct
    16501 actttcatat ctatagccaa atgaagcggt tgacagcgtt cgaaatatca ggaataataa
    16561 ccaatgagaa agattctcaa ttatgttagg aggaaagaaa tgaaattaaa aacaaaagta
    16621 ttcacattat ctagcatatt gctgctggct ggagtactga gcgcttgctc cccctcttca
    16681 gataaagaaa cagctacaac tgccaattcg acgacttcat ccaatagtac tcatacagtc
    16741 acagatacac ttggacataa agtagagatt ccaaacgcgc ccaaacggat catcggcagt
    16801 tacttagaag actatctgat tgcactaggg gaaaaaccta tcgcccagtg gactgttggc
    16861 agtggttcca ttcaagatta tctgcaagat gatttaaagg atgtcccaac gatttcttat
    16921 gaccttcctt atgagaaagt gttaagcttt gagcctgatt tgctgttgat ttcttcttcc
    16981 gctactgtcg aaggcgggaa atacgaacaa tatagcaaaa tcgcgccaac ttatgttgta
    17041 aaaaacggaa cagatgtcac atgggaagaa caactaaaag atgtcggcaa agctttggca
    17101 aaagaaaaag aggcagaaaa gatcatttcc gattatcaaa aatccatcaa gaaaactcga
    17161 gaagaactag cagataaaat cgagaacaag acagctgcag tgttgtgggt gaccaacaac
    17221 tcagccttta tggtcagcaa aaatcggtca agtggaagaa tcgtatacga cgatctggaa
    17281 tttggtgttc ctgatctagt ggaagaggtc tccaaaaaag ccacttctga ctggtctgct
    17341 gtttcctctg aaaagctagc tgaattagat gctgactaca tcatccttgt gaacagtgac
    17401 aaagatgctg ccatgttcaa tgaaccaagc tggaagaatc ttaaagcagt gacagacaat
    17461 catgtactgc aattcggacc tgaatctagt tggctatata acggacctat tgccagtcag
    17521 gaaatggtca atgatatcaa agaaaaacta aattgatttt aactaaaaaa agcatcttca
    17581 cgattagtcg tgatatgctt tttttcttat taacaaacta gacgcataga ttttattctt
    17641 cttttgaagc aaccctattt tttactcttt cccatttttg aaaacgatgg gcatatagat
    17701 tttcttgatc cgttatcccc tcatactcct ctataagggc gaagtctgaa aaatcaatat
    17761 tcccaatata cgtatcgcct tcaaacgtgc tatcgatcaa tgttcgccag atccgaccag
    17821 tgtaaggaag aagttcttga aaaatccgac taccacccga aacatagata tcttctgaga
    17881 tcgtttgact atacgacaaa agttcatcga tcgaatgcat tacttccgca ttttctttcg
    17941 tgcgaaaatt tggctgcgtt gtcaacacga tgatatggcg ataaggaagt gaaagatccc
    18001 ccattccttc ataggtcttt cttcccatca ctaaaattcg atccattgta tgttcgcgaa
    18061 agaaacgcat atcatttggc aatcgccatg gtaacaaacc atttttgccg atcagaccat
    18121 ttttatcttg tgcccacatg gaaataaaca tattatccgc tccttatgat tttagatgtt
    18181 ttgagataaa aaaagtctca attctttgac cagcaaggaa ttgagaactt taaaaacatt
    18241 attttgtttc acggtgcaaa gtaactttgc gttcgcgtgg gcaatatttt tgtttttcca
    18301 aacgatcagg attgttacgt ttgtttttgc ttgttagata gttacgttct ttacaagaag
    18361 tacactctaa agtgatgttt acgcgcatgt ttttccctcc catttttaca agaatcctct
    18421 aagatgactc tcagacttga ttatcttacc atgatttttt ctagattgct agtgtttttc
    18481 acgattttgt taagtaaaac tacttgacga taaatacaac ggttttactt ctcaaacaaa
    18541 atctttttct ttttgtataa atacccttac acttgtttat atagattatg ttttttaata
    18601 aagggggata ctaatatgcc atggaacatg aaggactttc ctgcatcaat gaaaaactta
    18661 gataaattga caagaaaaaa agcaatcgac atcgcaaatg cattattaga tgaagggtac
    18721 cctgataacc gtgcgatccc aattgccatt gatcaagcaa aaaaatggta tgaagatgct
    18781 agtgaatcag aaaaacgaac tttcgaaaaa gaaaaaaatc caagcaagac ggatgaacac
    18841 gatacgaatc ctcgtgctgg aaaactgctt gattcagatg tgatcgtaga atatgaggaa
    18901 gaacaatgga tcgtgaaatc taaaggggca aaaaaggcaa gcaaccattt tgatacaaaa
    18961 aaagaagcaa tcgaaaaagg aaagcaagta gcacaaaaca aagaatcaac tttgttcatt
    19021 tataaaaaag atgggacaaa agaaaaagaa atcgattatt gaacatatgt aaaggggttg
    19081 tgacgaaaat cgtgtcacaa cccccttatg ttcgaaacaa tggtgttcct ctgccaactc
    19141 cgaaccataa gtcataatat ctataaaacg ggaagagctg ttttttgaaa ttgtttctta
    19201 tcactcagtg ttaaacggta ggagaaaacc tcttttttat tgatatttga ttttcatcaa
    19261 acagtcacat agtcgattgc ttctgcttgc ggaatcgttt catctacaaa gtagacttta
    19321 taccatgtca agaaactgaa gattgtccag atttttcttt gctcttcttt acgtccttcg
    19381 tgatggtcat ctagcaactt catgattttt tcttgatcaa aaaattcttt tgcaaaatct
    19441 gcttcgaaca atgctttgac ttgttcgtag ccgtgttctt ctttcaacca agctttgatt
    19501 ggcacaggga atcctaattt cacacgattc gaccattttt ccggaagatg tttgttcgca
    19561 gcttgtctga aaatatcttt tgtgttgttt tgattcaata aaaacttact tggtatttgt
    19621 tgagccaatt tcatgacttc gatatccaat aaaggtactc ggacttccaa agaacttgcc
    19681 atagaaagtt tatctgcctt taacaagata tcttttggca tccattgatg aagatcgaca
    19741 tattgcatct tattgacttc atctttgatt ccttctgttt tctcgtaatg aacagtcatg
    19801 atttcatcca cagatggtgc tgtttgatat tttggtgtta acacttctga tgcttctgat
    19861 tcttcgaaca ctcgagcatg accgataaaa tattcttttg caggagcaag tgattcatat
    19921 aaatggatcc gtccatggaa atttttcatt tttccgattt tacgtcctaa attatagcgt
    19981 gttccttttg gtaatttttt caatccttga gcaatcacac ggatgaattt tgaattcgta
    20041 tggaatccat acgtttggta tcccgcaaac agttcatccg ccccttctcc agattggact
    20101 acccgcacac tttgtgaagc aagatttgct aaaaagtaca aaggtacgca agaaggattt
    20161 gaatcaggtt catctaagtg atactggatc aaagggaatg ctttgaacga catgtctccg
    20221 tcgatcactg ctgctgtatt atctaagtcc aatagttcgg ttagttttct ggcttcaatc
    20281 gcttcattat aggttttatc atcaaacccg attgagaaag aatgatctgg tttcaataca
    20341 gaggttacat aactggaatc aaccccgctg gataagaaag aaccaacttc tacatcactg
    20401 accgtatgtg cttcaataga agcttgaacg gtttcgtcga ttttttcgat ccattcttgt
    20461 cgggaatgcg tttcttttgt ttcaaaatct gcatcccaat attgctgaat gtctattttt
    20521 ccatctttat acgtataata atggccttca ggcaaacgat agacatcttt gaagaatgtt
    20581 tcgccgttta atggagaata ttggaaagtc atataaggtt tcaacgcttc tttgttcaat
    20641 tctttgttaa aatcaggatg aggcagaaaa cttttgattt ctgaaccaaa cataaatgtt
    20701 ccgttcattt ctgcataata atatggtttg atcccaaagt ggtctcttgc accgaataac
    20761 tcattctttt cattatccca aatagcaaaa gcaaacattc cgcggatctt ttgcaataat
    20821 tctgttcccc attcttcgta accatgcaaa aggacttctg tatctgcatg tgtttgaaaa
    20881 acgtggccag ctgcaatcaa ttcttctctc aaaggctggt aattatagat ttcgccatta
    20941 aaaataatga tctttgtccg atcttcattg taaataggct gagtaccgcc ctctaagtca
    21001 ataatactta atcttctgaa tcctaaagca acatgtttat cgatatattc tcctgaacta
    21061 ttaggtccac ggtgaacgat tcgatccatc attgtattaa taattgtttt tttgttgtcc
    21121 ttgtcgttaa caaaacctac aataccgcac atttaacttt cacatcctaa tataaaatta
    21181 ctgtttattt cattcatatg catcttatca tagaataaat agcggacgaa tattttctct
    21241 cttacaattt cgtcacgtga aaacaaaaga tgaaagtttc tgtttttaca gaaaataaag
    21301 tcccaatatc taatttacca tgttttcttt tttgaggcta ccttattctc ttcgtgttaa
    21361 aaaaatatta gtggatgcta tttccaaaaa aaccagcaaa aaaagacacg acctaaagat
    21421 cgtgtctaat tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa
    21481 ttgacatagt ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc
    21541 aagtcttctt tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa
    21601 taaatccctt tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttctttct
    21661 ccagtatcgc atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg
    21721 atactttcag attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca
    21781 aatcgagaaa cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa
    21841 acattcaagc tgatctggtt tgaaaaatga ttttctggtg ttccatttga tagcgagcga
    21901 taattgttca gattaaataa ttgataatca aatggcggat tatttgtcaa cacaccgaca
    21961 ggattatcat ataaatgaag tccatctttc atactttcaa ttactatcga tttttcttta
    22021 tcagctaata accagtgtaa aggtgacaag ggtagttttt cactaaaatc gatattgact
    22081 agattgatag tagccaataa ttctttagct tcgtctatgg ttgagcattg ccctagtatc
    22141 catggaataa actcaaatgg tgcaacgtta tcttttcctt cttgtaattc tttataatca
    22201 gcattgccag gaaaattcag tcctgccata ctcaaccctt tttcatttgt agcctcataa
    22261 tagagtggat agttggatac cccagctgct attcctatca ttgcataatg tttatctaaa
    22321 ttttttacct ttctaaaatc gaaacgataa tttcttggag taatagtgac cacttcatta
    22381 taagacatct cataatcaaa attccttcca aaataatggt ctttcgttgc ataagtgatt
    22441 gcggtacaca tcgtttcccc tcctaaaaca aatcgttttt acacttttat cttacatcta
    22501 gtattttttt tatcaaaaaa aggccaaaga agattgattt actagcattt ataaaaaata
    22561 gtactcaggg ggaatcaaaa aatattgtag gttaaaagta caacaataag gggctgtgac
    22621 aaaagtcttg tcacagcccc ttattccgaa caaacggtgt tcaaaagctt gcatctgcgg
    22681 taataagccc aatcaaacga aaaaatagga aaagctattt ttgtttggcc gttcttatta
    22741 cttgatgcag gacagcttct tcacaacctc taatctttga tataaggaag gtacaggatt
    22801 tgaacctgcg cgccggtatt agccggttcg ccggatttcg agtccggtgc attaccactc
    22861 tgccaacctt ccgtaagtac tatttttaaa cttttttcac gaaatgtcaa gcctatttct
    22921 aatagataaa taacagaaat cgcttttttt agcaaaaaag tccattgaca gtttgcagcc
    22981 aacggactta ccgatcaaat tcatactgat agaacactat ctgattattt caggattatc
    23041 taatgctaaa atcgagtcaa ttgtccgttt gatcagtgca atgatttgtt cttctgttgg
    23101 atgcatgatg tttgatgaca tcaatgacgc taagccagtc actgctaccc atgtaccagt
    23161 atgcagagca tcgacatatt catccgataa ttgattgtat ctttcatctt ctttaactat
    23221 ttcacggaaa taattgtatg aaaaatcatg cattttcttt ccgtccccat gttcatcaat
    23281 gtacaatgcc atgtaaagac tatgatgctt ttttgcaaag tagatatagt tcaaaccgag
    23341 atcaatgatt ttatcaccag tacgttcaat agggaaaaca tcttctttta gttgttcaaa
    23401 gatagaatcc agcaacgtat tttttaaatc ttccatgttt ttgaattcca gataaatcgg
    23461 ctgtgtcgaa atccccatct ttttagcaat atttctcgca gtaaatcctt taaacccttc
    23521 ctttgcaaca acatcataag cagcttttag aatttgatcc ttggtataga cttttcttct
    23581 cattaaaact cccccacttc tttactgcag tatatgttgt tttattgtac cctattttta
    23641 gcattttttg caaacatttt actcaaaacg ttatatttta aatttttttt cagtcttctt
    23701 taactttttt ttgcaaaatt aaaaaaattt ggttaaattc aa
//
LOCUS       DAZKOW010000039        23696 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000039 DAZKOW010000000
VERSION     DAZKOW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.039"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(24..1304)
                     /locus_tag="ACG912_001444"
     CDS             complement(24..1304)
                     /locus_tag="ACG912_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFQ0512773.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(1395..1847)
                     /locus_tag="ACG912_001445"
     CDS             complement(1395..1847)
                     /locus_tag="ACG912_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFQ0512774.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(1860..2270)
                     /locus_tag="ACG912_001446"
     CDS             complement(1860..2270)
                     /locus_tag="ACG912_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0512775.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            2545..2757
                     /locus_tag="ACG912_001447"
     CDS             2545..2757
                     /locus_tag="ACG912_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFQ0512776.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            2760..2972
                     /locus_tag="ACG912_001448"
     CDS             2760..2972
                     /locus_tag="ACG912_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512777.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            3112..3267
                     /locus_tag="ACG912_001449"
     CDS             3112..3267
                     /locus_tag="ACG912_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512778.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(3264..3488)
                     /locus_tag="ACG912_001450"
     CDS             complement(3264..3488)
                     /locus_tag="ACG912_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFQ0512779.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            3566..3754
                     /locus_tag="ACG912_001451"
     CDS             3566..3754
                     /locus_tag="ACG912_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512780.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            3751..3924
                     /locus_tag="ACG912_001452"
     CDS             3751..3924
                     /locus_tag="ACG912_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512781.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            3893..4021
                     /locus_tag="ACG912_001453"
     CDS             3893..4021
                     /locus_tag="ACG912_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512782.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            4080..4415
                     /locus_tag="ACG912_001454"
     CDS             4080..4415
                     /locus_tag="ACG912_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512783.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            4648..5589
                     /locus_tag="ACG912_001455"
     CDS             4648..5589
                     /locus_tag="ACG912_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFQ0512784.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            5591..6481
                     /locus_tag="ACG912_001456"
     CDS             5591..6481
                     /locus_tag="ACG912_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFQ0512785.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            6524..7324
                     /locus_tag="ACG912_001457"
     CDS             6524..7324
                     /locus_tag="ACG912_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFQ0512786.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            7339..8190
                     /locus_tag="ACG912_001458"
     CDS             7339..8190
                     /locus_tag="ACG912_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0512787.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            8391..8852
                     /locus_tag="ACG912_001459"
     CDS             8391..8852
                     /locus_tag="ACG912_001459"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0512788.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            8878..9330
                     /locus_tag="ACG912_001460"
     CDS             8878..9330
                     /locus_tag="ACG912_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512789.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            9436..9630
                     /locus_tag="ACG912_001461"
     CDS             9436..9630
                     /locus_tag="ACG912_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512790.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            9661..9954
                     /locus_tag="ACG912_001462"
     CDS             9661..9954
                     /locus_tag="ACG912_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512791.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            9885..10424
                     /locus_tag="ACG912_001463"
     CDS             9885..10424
                     /locus_tag="ACG912_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFQ0512792.1"
                     /translation="MVSEKQREFSSGEIGGHGSYKILILLFYNYPTTMSVIPQLEGEE
                     AIVNILENIDIKQTRKNARRLLKRYRNLERLVGPVKIDFSVMTVTKNLKFTIDSQNEE
                     IIEAISTRDSVIEALTRLSRIHFQVLYYSYCFPNKMSMYQIGEKLGYSDRTIERMKAV
                     ALVEFAEAYKSGELISRTK"
     gene            10499..10966
                     /locus_tag="ACG912_001464"
     CDS             10499..10966
                     /locus_tag="ACG912_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HFQ0512793.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQHR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            11713..11943
                     /locus_tag="ACG912_001465"
     CDS             11713..11943
                     /locus_tag="ACG912_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512794.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            12102..12935
                     /gene="terS"
                     /locus_tag="ACG912_001466"
     CDS             12102..12935
                     /gene="terS"
                     /locus_tag="ACG912_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFQ0512795.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            12928..14343
                     /locus_tag="ACG912_001467"
     CDS             12928..14343
                     /locus_tag="ACG912_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0512796.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            14355..15884
                     /locus_tag="ACG912_001468"
     CDS             14355..15884
                     /locus_tag="ACG912_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFQ0512797.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            15802..16854
                     /locus_tag="ACG912_001469"
     CDS             15802..16854
                     /locus_tag="ACG912_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFQ0512798.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            16856..17173
                     /locus_tag="ACG912_001470"
     CDS             16856..17173
                     /locus_tag="ACG912_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512799.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            17300..17923
                     /locus_tag="ACG912_001471"
     CDS             17300..17923
                     /locus_tag="ACG912_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFQ0512800.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            17938..18828
                     /locus_tag="ACG912_001472"
     CDS             17938..18828
                     /locus_tag="ACG912_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFQ0512801.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            18851..19066
                     /locus_tag="ACG912_001473"
     CDS             18851..19066
                     /locus_tag="ACG912_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512802.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            19078..19419
                     /locus_tag="ACG912_001474"
     CDS             19078..19419
                     /locus_tag="ACG912_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512803.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            19419..19790
                     /locus_tag="ACG912_001475"
     CDS             19419..19790
                     /locus_tag="ACG912_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512804.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            19783..20178
                     /locus_tag="ACG912_001476"
     CDS             19783..20178
                     /locus_tag="ACG912_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFQ0512805.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            20180..20557
                     /locus_tag="ACG912_001477"
     CDS             20180..20557
                     /locus_tag="ACG912_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFQ0512806.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            20558..21166
                     /locus_tag="ACG912_001478"
     CDS             20558..21166
                     /locus_tag="ACG912_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFQ0512807.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            21166..21507
                     /locus_tag="ACG912_001479"
     CDS             21166..21507
                     /locus_tag="ACG912_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512808.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            21749..>23696
                     /locus_tag="ACG912_001480"
     CDS             21749..>23696
                     /locus_tag="ACG912_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFQ0512809.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLN"
BASE COUNT         8427 a         3491 c         4742 g         7036 t
ORIGIN      
        1 aagcccgaag gctttttata tatttaatct tttgacaatt ctaaagatcg tctatattgt
       61 tcagctttag ctaattttgt aaatgttact gttccattat aattttctaa aacaactttc
      121 tctatttcgt ctagtgaaac tttgaaaaat tcttttcttt gattaatttt attaacttct
      181 tgttttctaa aatgattatg caatttagtt tccaaagacg gagcgtcctc tgaaaaaatc
      241 atcgcatgaa cgtcaaattc aaatggaacc gatgcactac tcaattcttt tattctatcc
      301 ataggttcta atcttctagt cataccaatt ttatatatgt cttcaccaaa agacccaata
      361 ttactaatta catacacata accggctcta gtatttaatt ctctttgtaa tacattttca
      421 cgtataactt ctaattcttt taatttttct tccagttctt gaattttatt tgcatataat
      481 tctttttcca catctgcatt agctttttgc atatatgcga ttaatttgga aatttcagaa
      541 ttgaattgac gttgttcttt atcaatacgt ttcttttcac gatcaatttc tctacgaact
      601 ttttcctctt ctatcatctg ttcttttatt gctttttgtt gttctttttc ttcggtaatt
      661 ttcactaaat aagagtaatg aagttctagt tcttgaagtt taaactctaa aagacccttc
      721 cttaattcag cattgtcatt cttgaataaa ttattaattt tatcaaatat ttttatgatt
      781 ttttccctag tcgagtctat attagaaata gttagtttat tgagtaatcc tttcacttct
      841 gaatcaaatg gaatgagcat ttttttcttt aaattatttt tctctcgttt atttaaaata
      901 agagggacag aaacagcatc gtcaataata tattcctttt cttttaattt taacaaagaa
      961 agcttatttt ttatttcaga agatgaagtt tcttctatat ttgaaacgat aagtgaagaa
     1021 tagacatttt ttaatgtttc atcatacttt tgatttattt catcatttaa atcttctttc
     1081 tcttttttta ataaaataat atcatttttt aacatagtaa tttgtttatt taaatctgat
     1141 agtgtttctt ccttacttaa tataaatctt tctctatcat gtaatttatt tagtttttca
     1201 tcattttttt gttctaattc ataagcttct ttttttattg tgttagcttt cgacagttta
     1261 gtgattccta taattaataa aataagtcca atcaataata acattcaaaa ctcctcattt
     1321 ctttgatata attattgtag agtttactca gaaatgagga aagagtccat gtgaaagcat
     1381 gggctttttt atttttaatg ttgtaatact aaatgttcaa gattatgagg aagccctaaa
     1441 tattcaataa tttggaactg actcatatca gataaatctt gttcatctat tgttctagtt
     1501 aacatttcaa ttgcaaacat attagcttca gcttctatac cataaatcat acaactaagg
     1561 ttgtttgctc taaaagctgc tgtgcaaata cctttatgga gtctaacgtg ggctatttca
     1621 tgccataata caaatctttt catagaaggt gataggtttt gattaattaa tatcacataa
     1681 catcttttat tgtgtatcgt ttgtcctaat gttttattcc ctaaatcagt ttcaattaaa
     1741 tctatatcag aatatttgat tattttatat ggattaatcg catcagatcc aaactctaca
     1801 aataattttt ctatttcttc taatatccag tatttgttta aatgcatacc aaagcccacc
     1861 tattgacgat attttttagg agtgaatttt ttctttgctt taactttggt taaggcaagt
     1921 gcaagttgaa tggcatcgta taatttttcc ttatcttcat cgtctaaagg ttcaccataa
     1981 taatttgttt cagcatctga attcaagcct tcaagtaatt tttcagcttg taaagcaata
     2041 tcttgtttat ctttttctgt taaatcataa taatggcgtt tatcagtacg tcctaaaaga
     2101 taatcagttg atacattaaa gtaatctgca actttttgta ttttatctcc agatggagta
     2161 cttttattcc atctagacat gcttccattt ccgaaattta actttctttc aagttctgcc
     2221 aacgtcattg aacgttcttt taataagatt tttatacggt ctaatagcat aggatatgca
     2281 ttcctttcaa aataccgaca aaaaaagtat ttttctgcaa attatctatt tacaaataga
     2341 gaatttgcag ttatactagt tttgtaagca agatatttac ggcatacaaa gactaaaagc
     2401 aataaactaa tagtctcccc agacatcagt aagctcgctt tttgtgcgct gtattttgta
     2461 tgcttacata atagcatatt tgcagttttt gtcaaactta ttagacaaaa atattagaaa
     2521 atattcgata agggggtatg aagcatgact ttaaaacagc aagtattaat tgctttagta
     2581 aagaaaggtt ggagtcaaag agagttagct cgacgaatgg gtatatcaat tacttattta
     2641 cgagatatct tcatcgaaaa aagaaaacct aaggaacgtt taaaacaaat cgaagaactt
     2701 ttggatatca agttagaagt agattctaat gatcagccat cagaacagga ggcgtaaaaa
     2761 tggtacaacg tttgagcaaa gcggagttta aagagaaata taagtattct gaaagtactt
     2821 atcaaagaag aatgaaggaa tttaaaaaat cacgttttag tgaggggtat aacgcagtta
     2881 cttccaaaga aataacaatt gatgtcgatt tatatgaaca gtttagaaca tggcgttcta
     2941 aaaatcgatt tagagttaga gctgtttgtt aatagctaaa aaatctaaat tcaccacttc
     3001 tgaaaagagg agttgctgct ctcctttcaa gtttgccaaa gtccacgttt tttcattttg
     3061 gcaactcctc ttcttggagg tggtgacggc gagagacgaa ggagggatga catgaaggaa
     3121 gaatcaaaaa tagggatttt gaatgagaac aattacacaa gattaattat tgaaacagaa
     3181 gaagggaaaa aagtagcaga agttacattg accgatgcaa ctcctgctga cggttatcgt
     3241 attcgattaa ctcctaaata tgattaacct tttggtgggt gtggatcgtt tccgtgacta
     3301 tcacggcttt gaatttttcc atctctgcca tgaataaata gctcagatcc ttgattttta
     3361 gaaatctctc tagcgatttt tactgcttct gattttgtat tagtatgtgc agtcgcttta
     3421 gaatttccag cacctttgac attccaacca ccattggaat taggtacaac atgttgattt
     3481 tttcccatag ttttcacctc catttctata gaaatatttt atcaaataga aatggagaaa
     3541 ttcaataaac gaaaggagaa gtgatatggc atataccttg caacaagaac atcaaattct
     3601 cggtttgatt aaacaacgca ggaaacaatt acaagatgac cgtgcagcgc ttagaaaagc
     3661 cgatgagcta tcagataggc aagctgaact aattgcttct gaacttgagg atttgagaat
     3721 gctagaaata aaaaataggg agattagatt atgaagaaga cagacacact ttttatagga
     3781 ttcattttgg ggttattagt gattgtagcg caccaaagta ttatcggggg aagcttgttc
     3841 gcagcattga tggttttaat caatctgctt gattcaaaag aaaggagcaa ctatggcacg
     3901 agaagaagcg ctaaaaatcg gtaaagtgat tgctgataat cggtggggaa atagccgccc
     3961 tattatttta agcaagcaac atatcgaaaa gcaaaaagca tggcaacaaa taaaaaagtg
     4021 actctgccag ccagcaaaga gtcacataca aatttataaa actaggagaa ttatatcaca
     4081 tgaataagaa aattgaaaat cttatcgaag aattgaaacg tgaatgccaa aagcaaggtg
     4141 tttctattat ttgtacagcc caaaaagaag gggaacttaa aagtctcgtt tatggtgaga
     4201 caacagaaat tttactttgt cttgctatgc aagaagaaca tttagatgaa aatctcccat
     4261 tgtcagcaca tattatgcgt agaattgctg tggatgctta cgaacaagcg aaaaatgaag
     4321 aagaaaatca accttcaaat catacttttg ttattaataa caaggaagat ttagctgatg
     4381 tgatgactcg tattctcaag ggggaatttc aatgatgaat gaaaaaacaa ttgatctact
     4441 cattatttta tttttcacgt tagtgattgt agcgaaaagt tatgtaatct ttggcttgtt
     4501 attcgcaatt accatgcttg tgctttgcgt gttagcaagc agaaaagagg ttaaacatga
     4561 acgagttagc aaatcttgac aactatttaa ctgatcctga ttatgctaaa ccaccatatg
     4621 aagcaccaat tgatgaggag gatgaagatg agtaaatcta ccttagaaat gagccatcaa
     4681 gaatggcttg aagaccgtaa gaaaggcatc ggaggttcgg atgttggaac aattttagga
     4741 ttgaataaat ggaaatctcc ttatcaacta tggctagaaa aaacaggaca agtagtactt
     4801 gaagaatcag gaagtgagcc cgcttattgg ggtaatgttt tagaagaagt ggttgctaaa
     4861 gagtttcagg aacgtacagg caaaaaagta cgcagaagaa accaagtatt tgaacatcca
     4921 ttacatccat ttttaagagc aaatattgat cgggaagtag tgggagaaaa tgccattctg
     4981 gaatgcaaaa cagccaatca atttctcggt aaagaatggg aaggcgaaga ggtaccgctt
     5041 agctatctct gtcaagttca acattacatg aacgttctaa acaaagacta ttgttacatc
     5101 gctgtcttaa tcggtggtca aaaatttatc tggaagcgga ttgaacgaga tcaagagctg
     5161 atcgatacaa tcactgaaca attagtagag ttttgggaaa caaacgttct tggaggtatc
     5221 gagccaatta ttgatggtag tcaagcgaca gctgatttct tgaaagagaa gtatgcagat
     5281 gttgaagacg ttcaaacgac gttaccaatt catttcgatg aactagttga acagaaaaac
     5341 gaactcaaac ggactaagaa agaaattgaa tcagctatcc gtcaagtaga caacgagatt
     5401 atcagcgagc taggtaaacg tgaagccagt atcggtatca ctcaaaaaaa catcatcagt
     5461 tggaaatttg tccgtacgag acgtatgaac tcgaagaaac tagcagagaa atatccagat
     5521 gtcgcaaatg atgaagagat ttataacgtt actgaatcaa gaaggctaac cgaaagggag
     5581 atcaaataat atggcaacaa atgaatcgtt aaaaaatcaa ttggcagcaa agccacagaa
     5641 acaagttgca ccaggtcaat taggacttaa agctttgatg aatacaccaa caatgagaaa
     5701 gaagttcgaa gaggtgcttc atgacaatgc taatgctttt atgtcgaatg ttatgactct
     5761 tgtatctaat gacagttatc ttgcagatag tgaaccgatg tctatcatga gtggtgcgtt
     5821 aactgctgca acattaaatc ttgggctaga taagaattta ggttatgcat atttagttcc
     5881 attcaatagt aaaaacaagc aaacaggaaa atgggaaaag aaagctcaat tcatgcttgg
     5941 ctataaagga tatatccaat tagcccaacg atcaggtaaa tacaaagcat taaatgtgat
     6001 cgaagtttac gaaggagaac taaaaagctg gaaccgactg acagaagagt ttgagtttga
     6061 tccaaatggt agaacgtctg atgaagtcat tggatatgtt ggctatttcg agttactgaa
     6121 tggattcaaa aaaactgtct attggaccaa acaagaaatc gaagctcatc ggattgctaa
     6181 caataaagat cgcgataaga caaagttaag tggtgtgtgg gcatctgatt acaatgcaat
     6241 ggcacgaaaa actgttttga gaaatcttct ttctaaatgg ggaatcttgt ccatcgaaat
     6301 gcaagaagcc accacatcgg atgagagagt ccaaagggtt caagaagacg gcagcattat
     6361 tgctgaaaca gaagttgaag aagatattcc tgaaagaaaa gaagcagagg ttatatctga
     6421 agaaaatgaa gatgtacaaa ctggattatt tgatgcatct aatccgccgt taaacaaata
     6481 atgagggagc tttctccctt gcttttctag aggagaaata cgaatgaaca caggatatgt
     6541 caaattacat cgaaaagtga tgaattcatt cgtttggacc aatccttata tgtataaatt
     6601 atggaacttg tgtttgatga aagcgagtca tgagaatcgc aagatacttt ttaatggaaa
     6661 agagatacaa gtgaacagcg gagaattcgt cacagggcgc gatgctatca catctgagat
     6721 gaacgaaggc gtgaaacgtg aacatcaagt gaacagcggt tctgtatgga gatggctaaa
     6781 acaatttgaa aaaaacggaa tgttgaacat caaatcaact acgaaataca gcgttatatc
     6841 aataaaaaac tggtctttgt accaaagcag tgaacaacaa atgaacatca aacgcacaac
     6901 aagtgaacaa caagtgcaca caaacaagaa tgaaaagaat tataagaatg aaaagaataa
     6961 taaaacatca tcgtcgcaac cacgcaaaaa gcgtgtttac gacaccgact cagtttacta
     7021 cattctcgcg gaggagttat tcaaacagat ttgtcagaat caggaaatca aaaagccgaa
     7081 tctgcaaagt tgggcagatg acattcggaa aatgatcgag atcgacaagc ggaccgaaaa
     7141 ccaggtacgg ggaatgattg aatggagtca gcacaatgtg ttttgggcat cgaatatttt
     7201 atctgctaaa aaattgcgag aaaaatacga cacaatggca gcgcaagcaa atcgtgatta
     7261 taaaacaaaa caaactaaaa cgcttgaata cgagaaattt agcacagatg agttgcctat
     7321 ttgagaggag gcgtaggcat ggaaactgtt ggggaaatca tggaaaagct gatacagaaa
     7381 gtgcttgtcc aacgtggcga atgtcctgaa tgcggacagc ctttgtatgg atggcgtacg
     7441 aagaaccctg atggctcaga acgttgtaag ccaacctgta tgcaatgtgg gtataaagca
     7501 ttgcgagttc aggaagactt acaaacagaa cgaatttaca acgagagcct gaaagcaaga
     7561 gcaatcaatt ttttcaaagg tggttctgtt gtgcctaatc aagcgttgtt tgattgcaca
     7621 ttgcagaatt atcaaattgt cgatcaagaa acaagacaag cggttgaagt aaccaaacgc
     7681 tttgttaatt cagtcttgtt aggaaatcca agtcaccttg ttttaactgg aaaacaagga
     7741 acaggtaaaa gtcacctagc gatggcagcg gcttgggaag tcttgaagcg atcaaactac
     7801 gataaaaaaa tcttatttat cgggttacag gaaatgctgg atcaaatcaa attttcgtac
     7861 aacaatcctg aactcagaaa aacgattgag ggatcgttga ttgcagatat caaaacagcg
     7921 gatttagtca tcattgatga tatcggttca gaactaggaa aagatgcatc agatagtcga
     7981 gcgtttggca taaacacgct aaattcgttc ttggatgcac gacagaacct agcaacgatt
     8041 atcacaacga acttgcttgg tgaagaactg aaaaaagctt acggtacgag aacaatatca
     8101 agaatgtttg tcaactctga tggatttacg atggtatttt ctcaaacagc agacaagcgc
     8161 ataaaaccag tcaaaggagc gatcgcatga atttgactaa acaagaaaaa agaatctgtc
     8221 gtcagataat gttgcaaatt ataggtgatc gtgacatgga cgaattatct caacaagaga
     8281 gtcgaatggt tcggaagata agtaagctaa ttatttatcg ggatacagaa agatttctca
     8341 ctgttttagg taacgtctac gagaatccag agttattgga ggtagagtaa ttgccaaaaa
     8401 tattagatgc atgttgcggt agtagattgt tctggtttga taagaacaat tctgacgtaa
     8461 catttatgga ttgtcgtcag cagtatgaag aattatcaac aggtcatgtg atcaatgttg
     8521 atccagatgt ggtcgcagac tttcgcgata tgccttttga agatgatgtg ttcgatatgg
     8581 ttgtatttga cccgccacac ttaagacatg tcggcgaaaa ctcatggctt gccaaaaaat
     8641 atggaaaatt agacgaactt tggccagaag acatcaggca aggttttgca gagtgtatgc
     8701 gagttttacg accttcagga acattaattt ttaagtggaa cgaggaacag ataccacttt
     8761 ctgatgtgtt agaggctatt ggagaacagc cattgtttgg taataaacga agcaaaacac
     8821 attggcttgt gtttatgaaa aaattcagct aacggaggaa acaaaggagg aaaagtaatg
     8881 aagattgtat taaataaatg ttttggtggc tttgggttga gccatgaagc aaaaatggaa
     8941 atttttaaaa gaaaaaatat tgaagtattc ccatacatca ataactttag ctatgactca
     9001 gatgatgaat acacgagata cacaggccaa aagctaggtt cgatggattt tatctactat
     9061 ttcaaaaaag acccgaaaat cgacaaagtt acaggaattt atagcgaaat tgaccgactg
     9121 tacggaattg ctgatgattc cagtttttca agtgattcaa acagaggtga taaggacctc
     9181 gttgctgttg tcgaaaaact aggaagtgag gccagtggac catatgcaag tcttaaagtc
     9241 gttgatatac ccgatggggc tgagtgggaa ataagtgatt atgacggcgt tgaaactgca
     9301 cattatggtt ttcaaacagg tagtatttag tccactaatg acaactaaag aggaggcaga
     9361 aggatgagtg aagatgaata ctttgaacag tagtctatta acgacagaga tttttaactg
     9421 gaaaggggtg tagtcatgga cgcatcggaa gtagtaaata aaattaatag tctcaaagag
     9481 gttttcggag acgtcgaagt agtgataaac gcacaaggct atgacaacga attttttaag
     9541 cagattaccg acattagcat tcagcaagga atggaagacg aagacggaaa ctttatcgat
     9601 gaagtagcca ttttagtgac ttgcgagtaa gtaattgaca gctaaagagg aggaagcgga
     9661 atgaaactaa aagacggatt ttactccagc ggccacggca tcggcggttt aatgctagat
     9721 atgccgacaa agaatcctaa aacacgtaag aaaccaaaat tcaaagtcgg tgacatggtt
     9781 cgctgcgaag cagaaggatt catctatcca tttcgtggat atgtagaaca cgtctataat
     9841 cactcagcaa tcattcgcat tgaaaacacg atggaatgtg ataagtggtt agcgaaaagc
     9901 aaagagaatt tagcagtggt gagattggtg gacatggaag ttataaaatc ttaattttgc
     9961 ttttttacaa ctatcctaca acaatgagcg ttattccaca actggaagga gaagaggcaa
    10021 ttgtgaacat tttagagaat atagatatta agcaaacaag gaaaaatgct agacgattac
    10081 taaaaagata cagaaattta gaacgtttgg tcggaccagt gaaaatagac ttttccgtga
    10141 tgactgttac caaaaatttg aaattcacaa ttgacagtca aaacgaagaa atcattgaag
    10201 cgataagtac tcgagattcg gttatcgaag cattaacacg gctaagtaga atccatttcc
    10261 aggtacttta ttatagttat tgttttccta ataagatgtc aatgtatcaa ataggagaaa
    10321 aattaggata ttccgataga actatcgaga gaatgaaggc agtagctttg gttgaatttg
    10381 ctgaggcgta taaatcagga gaactcattt cacgtacaaa ataaaaaaag tcggattcct
    10441 ccgactatga ataatatttc cgacataagt attatatcat aattggggga atcagaggat
    10501 ggtacttttc gatgtaaaga aatatgagac accaagcgcg aaagatgttg atatggaacg
    10561 cacaaaacat aatgtcgctg tgtttctttc agcatatcta tcagctagat gtagagtagg
    10621 gcaacatcga gaaccaaaag tgacagcatc ttattccttg gttccacctt ctacagctga
    10681 tcatatattt gaagcagaaa gaatgctgat cgataaagaa gaagcacagg aagaatttga
    10741 gtatctgcat aaattgtttg ttcgaggcta ttcagcgata cagcatccgc ataagcctga
    10801 tgtgactgaa aggcgcaaga agatattcta tgatcgttat atcaatggtc tgcccattta
    10861 tgtaactgct caaaggaata atactagcga agaatcggtt aaagtagaat caaacagaat
    10921 tatcatccaa tttgcttcat cgttagaact ggttgctttc aagtagccag tttttacact
    10981 ttttataccc ttttattacc aatttggttt ccattttata ccttttttat accaatcact
    11041 tacctattca acgttgtatt atgatagtgt cgaaaaatta ggaaacagga tcgacaaaat
    11101 aaaatgtaag ggaggaaatc tccctcatcg tttaattaag cttcgatagg cagcaacgga
    11161 aatattaaga ataaggatgt gaatttcaac tccttctaaa ttgttcttat tatctatcat
    11221 ccgttgctgt ctattgtttt tttaattatt cacacgataa ctaaaggtaa gtgaagagaa
    11281 atgattccat taataatttc aatttttgcg ctttgtctta atgtatatat gattggattt
    11341 aaaaatgggc aaaataaaaa atagtagcag ccaagaataa ttttatagtg tcactgtggc
    11401 ggaaagggta gacgctaagc atgtgtgcta ggtcaatgct tcggcaacca tgcaaggttc
    11461 gattccttgc cagtgacttt ggcaaccgag gcatcggcgg tttaaaaata taggggtgcg
    11521 caatttcgta cgcgttttgt gcatcgtgta ggttgctatt acataactgg ttaggttaga
    11581 ttgagatttg ggattcggta caaatgaatc gtcaaatgtc tcaagcacag gatcggaaac
    11641 gtccctgcct gtgcattaca tattagatca ctctttgagt ggtctttttc tttacataaa
    11701 ggaggctaca taatgagaaa ctactggtat gtatcgctaa ctaatgaata tcctcgaacc
    11761 attgatgatt gttcagtgcg tgttgtgcgt tctgtacaaa tcaaagggaa gtactctatt
    11821 gtcgaaatgg ggagagaagc agaaccatgt gagattgatg cgtgcaagct cgtttattgc
    11881 ggtcatggtt tctatgatga accaaacatt caaaataata ttaacaagaa tttgagggat
    11941 tagaatgcaa gaaatggctt aaaaatctcc aatcaggaca aatcaagtta gacaaagttt
    12001 ccgatttaaa gatattgatt gaagctgatt taatgctaaa agatattgaa aattagaaaa
    12061 caaaactcaa cctaaacaga atgtgaggtg gtgtgtattg aatggcaaga caacgtgatc
    12121 caagacgtga tgaagccaaa agaatttggt tagaatccaa cggagaaaag cagttaaagg
    12181 aaattgcatc tgaattaaat gtttcagatt ctcaggttag aaaatggaaa tcgcaagaca
    12241 aatggagcgc tgaattgatt agtaacgtta ccaatggcaa aagtaacgtt actaatcaag
    12301 gtggcgctcc tattggtaat caaaatgcta aaggtaacaa aggaaatagc cgagcttctc
    12361 cgccagtggg taataaaaac gctttgaaaa caggtgagta tgaaacaata ttttttgaca
    12421 cgttaaccga taatgagaag gacatctatt ctagtttgaa tgatgatcct tcttttgctt
    12481 tgtccgaaga aatacgactt cttaaaataa gacaacttcg aatgatgaaa agaatccaac
    12541 aagctgaagc tggactaaat gatgaagaag tcgaacgatt gcagcagcta agaaagatta
    12601 aaactccgat tgaaaaaaat ggtaaaaagc tagaaatcaa gcgtgaggtt atgcaagatg
    12661 tgcagattag cagaaaaaaa catcgcaaaa ttgatgatat tctttcaatt gaagattcat
    12721 tgactcggat tagcaaccag ttagctaaag ccatcaagca aatgaatgaa ctttatatga
    12781 atgaatacag aactgattta attaaagctc agactgataa gatccaagct gagacaaatg
    12841 aaattggcgg aaataattca ggtgaagaaa tagaagaatg gaaacaggca gttttaaatg
    12901 ccgcaaacaa acgggcggtg aaagaaaatg aataatgaat ttattccttt tgctgatatt
    12961 ggttctgcca ttgattatta ctatgataaa ccagtagctt tttgccaaga tattttgcat
    13021 ttgaatcctg atgaatggca agaaaatgtt ttaaatgatt tagctgaatt ttcaaaggtt
    13081 tctgttcgtt ctggtcaagg agttggaaaa acagcattag aagcaggagc aatactttgg
    13141 ttcttaacgt gtcgacccta cgctaaagta atagcaacag ctccgacaat gaagcaactt
    13201 tacgatgtac tttgggcaga ggtagctaaa tggttaaatg atagcttgat caaaaactta
    13261 ctgaagtgga caaagaccaa aatttatatg gttggtgatt cagagcgttg gtttgctacg
    13321 gctagaacag cgactaaacc agaaagtatg caaggttttc acgaggacca tatgttgatt
    13381 gtggtagatg aagcttctgg tgtgtctgat ccaattatgg aagctattct tggtacgcta
    13441 tcaggttttg ataataagct gttgatgtgt ggaaacccca ataatattga aggtgtcttt
    13501 tatgattccc ataattcaga ccgtgataaa tacagagttc ataaagtatc aagctatgat
    13561 agtaaacgta caaacaaaga caatatagaa atgattctta aaaaatatgg aaaagaaagt
    13621 gatgttgctc gtgtccgtat ttttggagaa tttccgaaag gtgcgttgga ttcatttatc
    13681 agtcttgaaa cagttgaatt ggctacagaa aaacaaatta gtgattcttt agtcaataaa
    13741 acaaaggttg ctcatattgg tgtcgacgta gctcgatatg gtgatgattc tacgattctc
    13801 tttcctagaa ttgctaccag ggcattggag tatgagaagt attcaaaacg tagcaccatg
    13861 gaaacaacag gatatgtcat caacatggcc aagaatctaa tgagtcaata tccgagtatt
    13921 gataaagtga tgattaaagt cgatgacact ggtgtcggag gtggtgtaac cgaccgccta
    13981 gaagaactta tagaagacaa acattatcct tttgaggtgt ttggagtgaa taacggttca
    14041 acatcagaag acgattttta cgataattta ggtactcaac tatggggaaa catcaaggaa
    14101 atgttagaag aaaatatgac agcaaatctt aacggagaac agcctgttat tgaattgcct
    14161 tctgatagtt cgttaatcaa agaattaagt actcgcaaat tcaaaatgac aagtagaagt
    14221 cgtatacgtt tagaaagtaa agatgatatg aaaaagcgaa atattggtag tcccgatatt
    14281 gctgacgcac tggctttagc gttttatgag ccaccaagtc actatcaatt tattcaattt
    14341 taggaggtga gcttttgatt agtacagtat tatctttaga gagatataag aagctacgtg
    14401 ttaaatatgc aacacaaatt gaagatggaa tgtttgatcc gaatggtttt atagaagata
    14461 tgaaaccatt ttttgctgat cgcgaaagaa aatatctagc ttatactagc gaaaagaatg
    14521 aaatagataa tagaccaaag cctaacactg atattgtaaa agttaataat aaacttcatg
    14581 ctggtatgta ttcaattgtc gtagaccaag cagtcaatca ttttactggt atacctatca
    14641 aatgggatta cgatgtatcg gaacaaaaaa gaacactcat tcaaagatta aaagataaat
    14701 tcttaaaaaa tgatatagaa cttccgacag ttccagaagc ttttaacaaa ttaacaagca
    14761 atcttgattc catgagattc gcaatgcttg attctgaaac tgcaactttt caaggagctt
    14821 gtggagtagc tttccgattg ctagagcctg tggaagaaga tgatggttgg aagttaagag
    14881 caagtaatat tgaaccttgg agagcagaaa gatatggaaa tgccggaatc tatatcaaag
    14941 aaaagtatga ctcttaccag aaaaaatttt ttcaagaaat gaaagttatg acaagaaata
    15001 aaattctgac atatgcttgc tatggtgatt tgaattttgt tacaagcgga acgttcaaaa
    15061 aaattgatga gaccgataac cctttgggga cgattacctt ggcagaattt aaaaataata
    15121 cgaatcgtta ttgtgatttt gaagtagctg aggaaattgg agatgcaatt gatcgtgctt
    15181 tgtctgatca acaaaacgaa atcgaacaat ttaagcttgc gtacatgctt gttactggca
    15241 caacaatgag caaaggcact gctaaagaaa tgatgaatca gctagggatt atcaacttaa
    15301 aagcccctac tgcaaaagct gaatatgtca caaaaaattt agcaaaagat ttcaatgaat
    15361 atcatatgga tctattgaaa aaacagtttt acaccatttg taaagcaatc gattttaacg
    15421 atgaggtgtt taaatcaaac agttctggag aagctcgcaa gtggcaaatc attagcctag
    15481 aagcgaaaac aaatactaaa gagcaatatt ttagagaagg attgaaagaa tgcgcagaaa
    15541 caattgctgc ttttcttaag tttcatgaca aagtagaaat tgagcctgaa aaaattattt
    15601 ttactttctc tagatctttg ccaactgatt taagttattt agctgaagct ttacctaaac
    15661 ttgcaccata cgtatccaaa cgaactattc agagccaaat tccatttgta acagatgttg
    15721 attatgaaaa tgaaatgatg gaattggaaa gtggaagtgc ttatccagat agtgaataca
    15781 attttggcgg aggtggcaat agtgacgata gaaacgaagt attggaccaa acgtcgagaa
    15841 ctggaggatc aagcaaggct caaacaagaa aatcagacac ttaaaaaatt aactagtgta
    15901 tttcctgaag cactgaaaga gatacaagca aaactattat cacaagctga cttacacaat
    15961 atcacttatc cagaaatgat ggagttttac agtacaagta atcagaaaaa atatcgtgaa
    16021 tatgtggaaa aaaattataa gtcattaaaa atgtatgatg caaaatacaa agagtttatc
    16081 gatgaatttt ttccaccatt tgactatgca aaagtcaatc gcttattaca aatacgatca
    16141 gatgtattta aaattcttgc agaatatgcg atggatgcag atgtgaatca atatttttct
    16201 gatcgcttag aggaaattct tcaaagaaca tattcttcta atgctaatgt ttttgttcaa
    16261 cttttaaacg ttgatatacc aaattattta ccagaaaatg aaattaaaca ttatttgaac
    16321 tatccatggt caggaaagac attttcaaga agactttggg gaaatatttc atcgcttgaa
    16381 cagaaactct ctaatgctat tgtaaaaagt gttgctagtg gagaaggcgt tatacacgca
    16441 ttaaacacca tgagattaga ctcagaaatt tgtgacatgt ttaagttaga agaatcaaag
    16501 tataacaaag cgatagaaaa tctcgttcga acggagtatg caaaatttgc acaagatggt
    16561 attgaaaaat catatttaga aacaggtatt gaggaataca acgtattgac tgcaaaagat
    16621 gagagagttt gccggatttg tggaggaaag gcaagtaaga atccctataa actgaaagat
    16681 gctgtcatag gtgaaaatcg agcacctttc catagtcgtt gtagatgtac ggatgttcct
    16741 aatttaccaa aattaggaaa ggatattgat gaagaatatg accgtttatt tggcgattta
    16801 ttagatgagt ttgcacatga ttcttttgga attaatttga aacggaggaa gtagaatgaa
    16861 agattttttt gaagcagtac taacaattaa tgtaaatgct gatattgcag aagcctacaa
    16921 aacagctatt gagtctgaga accatcctaa tggcttgaga gaccattgga atggcaatta
    16981 tgcctacgtg gttattggcg atcaaactgt taattatcaa gataatactc cagttgataa
    17041 gaataccgtt aatttaacga ttcaattatt atctcattca ttaccaaatt taaaagaaac
    17101 agttgattgg tatgaaaaga tgggatgtat tgttgttaga actgactaca aagaaggaaa
    17161 gtctagtaat taggcttttt tattttgtcc gaaatgacgt taaactagcg caatactggg
    17221 cttggttgaa tggtggggcg caactattaa aactcaaagc aatgcggggc gtgaaaacga
    17281 atcgtggggc gaaaggagaa tgagcatgaa acacaaatca ttaatgccaa tgaatttgca
    17341 atactttgcc gaaggtgacg atcaaaagtt ttcttttgat gacttcaaat cttttgtgga
    17401 atccaatgaa gaagctcaaa aatttattca gtcacaatct caaagtgttg ccgacaaaca
    17461 attggaagct tggaaacaaa ataacttaga taaaattaaa caggatacca tcaaggaata
    17521 tgaggaatct aagaaaaaca agtcacctga gcaaattcaa ctggaaaaac tacaagctga
    17581 atttgaagca gaaaaagcgt tgcgtgtgac aagtgataat aaagcatttg ttgcagaaca
    17641 aattgctgga ttagaactag atggagagtt aaaagagtct atttctcaat ttatgctaaa
    17701 taatcttgtt agttcggata cagatttcac taagaatgct gttgaaggtt ttacaagtgt
    17761 tttgaatgca attaatgaga aacatgcaga tgcattaaaa gaactacaaa tgaagtctgc
    17821 atttggtgga actcaacaat cgaataacca ggttcagcag aacaatgaaa cattaacaaa
    17881 tccagaagaa caattaggac aaattcttca acaatttaac taggagagtg aaaaattatg
    17941 aaaaaaacat ctttaaataa tttagagtat ttggatattt caccagcgat taatgctatg
    18001 caagtaccaa atacaccttt tttaagctat ttacttggtg ctggaaaaac agagccagca
    18061 aactcgacag aaattaaatg gcgtgaatat gatatcaaca acgatgattc ttctgaaaaa
    18121 cttgagggcg gagaatatcc agatgctgaa tcaggtcgaa cttggtttaa caactatact
    18181 gaaattttta gaaaatcaac ctctgtatct ggtacattag atgctattaa tgtgaatgga
    18241 gtcggaaatg aattaactaa tcaagtagca ctacgtggta tggaaatgaa aattgatttg
    18301 aaccgaaaat tgattactgg tgtaaaagct gatgaaaatg gttctaaagg tcgtcgaatg
    18361 aatgggattt tgaacttgat caattcagca aataaggcag aaacagccac tgcgggtgca
    18421 gtaacaagaa aagatatcga tactttattt aaattgatgt atgaaaaagg ttatatggga
    18481 gaaaaactat gcttgatttc tccagatatg caggagttaa tgactgatga gttagatgga
    18541 aaatcaacaa aaattgttca gttcggggaa agagtaactt ttggattgca aattggaaat
    18601 atcgtgtcta attacggtac aggtattgct ctactagaac catcattgcc aaaaggaaca
    18661 attgccgcaa tcgatactaa ttatgtgaaa ctacgtccat tacgtgaatg gagagcagaa
    18721 gaacttgcaa aaacaacgga ttctagacgt atcggtcttg taggtgaata ctctcttgaa
    18781 tacaatgctt caaactctgg ggcaatttta aatttaaagt ctgaataaaa gggagttagt
    18841 actccctttt ttgataggag gaattacagt ggtaaaaaaa gatgaaacta aaaaagatga
    18901 agtcgtgaaa tatagagtag gtaaaactaa aaattttgtt gggtttgttc atcctaaaac
    18961 tcgtagattt atcacagcag attcaaataa cgaatttatc atttctatag atgataaaga
    19021 agcaattgca attttggaag atgcaattga tgttaatgaa atttaggaag tgatctgatg
    19081 gatgaatcgc taaaaacgga aatcattgag tctacaaaag aagattttcc agatttgagt
    19141 gaagaacgca taactaattt attagaaata attttgctag aaattgaatc atacaacact
    19201 tgtaaaaatg atatttcatg ggaaaagtta aaaagcgtga ttagtgaagt gttgtataaa
    19261 ataataaaaa atgaatcaga aaaaacagta tcttcagtta gacgtggcga tacaacgatt
    19321 agttatgctt caacaacaaa tgatgttagt gagttacttc taggctatgg tgatttgata
    19381 cgaagagtta ttggttgtgg aggattggag tttttttaat gaatgaagca gatattttag
    19441 aaactactta tgaagatagt tgtattattg aaagactgac ggacattgaa gattctaata
    19501 caaatattac tattcaagat tacaaaaaag tatatgataa tcctatttct tgcgctcttt
    19561 cacaaggtca aatcgatgga ctagcagtca tagaagatgg agagatggta aatgtttcaa
    19621 ctgacacata taaattattt gttcatccta agattaaact aaaaaaagga gatcgaataa
    19681 caataactca aaaggccagt ggcttaattt tttctctatt tgctactaag cctttttact
    19741 atcctagtca ttgtgaagtg aatttgatag gaagtgagaa aaatgggtaa tctcaaatat
    19801 gaatcaaatg cagaaaagat cattgagaat tttaaaaata tgactgtaat tgctcaaaaa
    19861 gaaggaatat ccttcgttaa cgattcaatg aataaagtcg ttagtttagc taaaccatta
    19921 actcctgtaa aatcaggtaa tttaaggcga ggatatcgtg tagtaaaagc tagaaaacta
    19981 tcaagcggtc gaattgttgg agaagttata aacaatgaac attattttaa atatgtagaa
    20041 gaaggtcata ggactaaaaa tggtggcttt gtcaaaggga aattcatgtt aactcgtgca
    20101 acgaatcttg caaatatgtc ttatattcct cgaagattta aacaaatggc aataaaaatc
    20161 gttaagaaag gaaagtaaca tgaaagatga aatcattgct gcaatcagta gcaaattaaa
    20221 agaaatctat cctgatggga caatatatct tgattcagtt atgcagtcaa ctaaagattt
    20281 ttatttcgtt ttatcagtaa tggaatctgg aactgaaaat gtaggaattg atattcaaaa
    20341 tgtttctttc ttaattgata ttgcattgat tgataataac cctaatagaa atttaattaa
    20401 tgaattagtc tcacgttgtg ggactttttt taatacgatt acaatcgatg agcaaatatt
    20461 atttccaaaa gcctatttac ctgatgaagc agatggtgtt caacatattc gttttacatt
    20521 agaatttcca caatatattg aatggagtga aagataaatg ggagaaaaaa gaagtaaaac
    20581 cggaattatt tctgtcgaaa aacctacttg gtttcctttg gaagatgaga cagggaattt
    20641 tccggtatat ggtactgcgt tgccaatggg aacagcagta agtatcaaac caacagctaa
    20701 ttatgaaaca acacaagatt atggggattc agtggttcaa gatcaattta cagcttttgg
    20761 tggtgcagaa gttgaactgg aagcaaatgg atatactcat aaagttttat ctacaattac
    20821 tggagggaaa attgttaaag gtggtgcctt gcgttctgga gaagatattg cgcaagatgg
    20881 tgcgtttgca tatagacgta aaaaatcgaa tgggaaatac cgttatacgg tcttttataa
    20941 aggacagttt gctctagatt cagatgaaac atcaacgatt gaaggaagta aagtaagttt
    21001 tactcatcca acttggaagg gttcatttgt agatgtacca ggacttggat atatgtattc
    21061 agttgatgaa gatgatgaag gcgtggataa agcaatgatc ggaaattggt ttacaaaagt
    21121 agcaattcca attgaagaag cagaactttc aggaggtaca gaataatgtc aaaatatcaa
    21181 acaacgatta aattaacgaa aaaagacgaa gaaggtaaat atgagcaagt acaatttaaa
    21241 tctgctgaat ttttaccagg aacagttgta gaagatgctg caggagttat ggaagaaatg
    21301 caaacagcga ctgataagca atcagttaaa aaagctttaa gtcgtgctta ttcatttatt
    21361 gcagatactt tatttgaggg acaatttact ggtgaagatt attgcaaagg aattgatgct
    21421 cgtgagattg cctcattgac aggaaaatta ttgaagtctg ttactgcagg ttttgatgaa
    21481 acttatacag agacgaaaaa aaagtaagtg aggcgctcaa gtcaccttca tttaagtatt
    21541 cgattactta ccgagaatta gatataaaga cgcaattact cgaagcaggt tggacgttac
    21601 cagaaattga acgtactgac ttggatgagt taatgcgtct ttttgctttt agagatgcgg
    21661 taaaagagca tgaagatgtt gagtactatg ataatttcac tcaattttag gaggtgatac
    21721 tttgaataac gatgacctaa ttctgaagat gatactagat gaatctggtt ttactgccgg
    21781 tatgaataat gctgtcaaaa agttgaactc ttttgatgaa acaatcgaaa gaaaaagtag
    21841 aaatagtggc agttcattag gtagtatttg gaaaatattt gctggaagtt ttctagctag
    21901 cggagtaact agaatcgtag gagctggctt tgatctaatc aagggttcca taagtggagc
    21961 ggttgatcga gtagatacga tgaataacgc tctacgaaat ttccaaaata tgggatttag
    22021 taattcagaa attatgaaga atatcggaaa gaatgggctt ttatctcaag gtattcaagg
    22081 acttcctact gctttgaatg atgcgataag tcatgttcaa cttcttgctt cttctacagg
    22141 tgatttaact cgttcaactc aaatatttaa agctttaaat gatggaattc ttggtttcgg
    22201 tggttcaact gatcaagtta atgaagctgt tattcaatta tctcaaagtt tctcaaacgg
    22261 aaaagtagac gcacaaactt ggaactcaat gatcaacgct caacttggac ctactctttc
    22321 tgctattgct aaaaagatgg gaattacaat gggagatctg aaagaaggtt tatctcaagg
    22381 taagatttct gttgaagaat tccaaaatca attaatagaa atggatacca aaggtggcgg
    22441 aggacttaaa tcattaagtc aaatcgctaa agattctact aagggaatta aaacctctat
    22501 acaaaatgca aaaacagctg ttacacgtgg tgttggtgag gtaatagaag gattaaataa
    22561 agcattagta gattcagatt taggtggatt taagggaatt attgataaag tgggcagttc
    22621 aatggaatcg ttcttaaaag taattgctgc aaatattcct acagcagtat catttttaag
    22681 taatttattt aacgaaattc aaaaatttgg ttctgctttg aaattcatga tgccctttct
    22741 tgttcctgca gctaccgctt ttggagcgtt catgtttcaa cttaaaggta taccagcaat
    22801 aataaaaagc ttcaataatt ttaagaatgc cataattggc gttggaaatt cattaaagat
    22861 tatgggggca atagctgctg ctaatccatt tgttttaatt attggtgcag tcgttggtgc
    22921 aatagctgtt ttcggttact ttatggcaac caatgaagag cttagaaaca aggttatatc
    22981 tatttggaat gatgtgaaag attccgtaat tggtgtatta aaaaatataa aggactgggg
    23041 aattgatact tggaattctg ctaaagaaat ggcatcaaat gcagttgagg gtgtcaaaga
    23101 tgcttggtca ggaacaaagg aatggttttc taacacctgg aaaggtatta aggatggggc
    23161 cacaggttta tttgataaaa cagtagagac ttcaagaaat gcagtcgata gtgtaaaaaa
    23221 tgcatggtct aatatgaaga aatggttttc tgatacttgg caaagtataa aagattcagc
    23281 cagagaaaaa tggaatggaa tcaaaggttc tattatgtca gttgctggtc cattgattac
    23341 aggtataaag aatgcatttt tacatgttac tttttatcta gagaccttat ggaataattt
    23401 agttgagatt ggtaaaaacg tctttgagat tttaaaaaat gttatacttg cgccggtttt
    23461 gtttattacc tccatgattt ctggtggatg ggaagaaaca aaaaataata tgattgctgt
    23521 ttggaataat attaaagaaa gtgccataaa tatttgggaa tctataaaga atatatttgt
    23581 aagttatttt acaaatattt attttgctgc actcaatatt tggacagggt tcaagcttac
    23641 attgataaat atttggaatg aagtggtaaa tcaagctaaa tcgatttggt taaaca
//
LOCUS       DAZKOW010000040        23144 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000040 DAZKOW010000000
VERSION     DAZKOW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.040"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1083
                     /locus_tag="ACG912_001481"
     CDS             <1..1083
                     /locus_tag="ACG912_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFQ0512810.1"
                     /translation="TQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTK
                     ELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVLCMDEFK
                     SMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMDMNAS
                     YCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYW
                     KLLLKDSTTLEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKR
                     DTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNGVTEGLNNKIKLIK
                     RIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            complement(1212..2603)
                     /locus_tag="ACG912_001482"
     CDS             complement(1212..2603)
                     /locus_tag="ACG912_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512811.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            2860..6249
                     /locus_tag="ACG912_001483"
     CDS             2860..6249
                     /locus_tag="ACG912_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0512812.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            6252..7673
                     /locus_tag="ACG912_001484"
     CDS             6252..7673
                     /locus_tag="ACG912_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0512813.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            7670..9547
                     /locus_tag="ACG912_001485"
     CDS             7670..9547
                     /locus_tag="ACG912_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0512814.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            9639..10481
                     /locus_tag="ACG912_001486"
     CDS             9639..10481
                     /locus_tag="ACG912_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0512815.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(10633..11508)
                     /locus_tag="ACG912_001487"
     CDS             complement(10633..11508)
                     /locus_tag="ACG912_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0512816.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            11715..12745
                     /locus_tag="ACG912_001488"
                     /pseudo
     CDS             11715..12745
                     /locus_tag="ACG912_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            12834..13139
                     /locus_tag="ACG912_001489"
     CDS             12834..13139
                     /locus_tag="ACG912_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFQ0512817.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            13318..15333
                     /locus_tag="ACG912_001490"
     CDS             13318..15333
                     /locus_tag="ACG912_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0512818.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            15335..15619
                     /locus_tag="ACG912_001491"
     CDS             15335..15619
                     /locus_tag="ACG912_001491"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0512819.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            15645..17018
                     /locus_tag="ACG912_001492"
     CDS             15645..17018
                     /locus_tag="ACG912_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0512820.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(17101..19116)
                     /locus_tag="ACG912_001493"
     CDS             complement(17101..19116)
                     /locus_tag="ACG912_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFQ0512821.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(19138..20352)
                     /locus_tag="ACG912_001494"
     CDS             complement(19138..20352)
                     /locus_tag="ACG912_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0512822.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            20572..21516
                     /locus_tag="ACG912_001495"
     CDS             20572..21516
                     /locus_tag="ACG912_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0512823.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            21812..22756
                     /locus_tag="ACG912_001496"
     CDS             21812..22756
                     /locus_tag="ACG912_001496"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0512824.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
BASE COUNT         7844 a         4260 c         4363 g         6677 t
ORIGIN      
        1 actcagatgt tgaaagtcaa agaaaagttg actattttag aattaaaacg tacccgtttt
       61 ctctgccatg attgtggacg gaccttttct gcgaaaacgg acttagttga tgaacatcat
      121 caactaacaa aggaactgaa gcaggcgatt ctcatggaac tgtacgaaaa tcaatcacgt
      181 aaactaatcg ctaagaagta cttcgtctcg gatgggacgg tgacacggat cttgcgtgaa
      241 gcaacgaagc attaccagcc acgtatgaac ttccttccga cggtgttgtg tatggatgag
      301 ttcaaatcaa tgaagtctgt ttctggttcc atgagtttta tttgtgttga tgggacaacc
      361 aatcagctat ttacgatcct tgaagatcgc agactttaca aactaactca atacttcctg
      421 cgctttccta gaaaggcccg tttaaaggtc aagtacctag tgatggacat gaatgccagc
      481 tattgtcagt tgcttaaaac agtctttcca aatgcggaga tcgtaactga ccgctttcat
      541 attgtccaac acatcaatcg cagcttcaat caattacgtg tccagatcat gaatcgtttt
      601 cgaacctcac actctgaaaa tcagaagaag taccgtcggc tcaagcgata ttggaaactc
      661 ttactcaaag attccacgac attggagcca ctcaaacggc attatcatcg actgtttaaa
      721 cgaccaatta gtcaaacaga aatcgttgat gagctgttaa gctacaacga agaattgcga
      781 actgcgtatc atttttgtca attattacgc tattatttcg ttaaacgaga tacggaaggc
      841 tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa aaagaaattc
      901 acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgcc ccactcaaac
      961 ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc gtttggttat
     1021 cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat ctttgaaaac
     1081 taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta ttcaaatttg
     1141 tcttcgccaa cacaatttga cgaagagcca tttttatcag ggtggtttat acatagctgt
     1201 tttttgatta ttcataactc gaataagcgg atgagttctt cttcatttgc gaatgacaca
     1261 ctatcatcaa tcaatatatg aggaacatgg gcattcaaaa tgggttgatt cgtaacgatc
     1321 agatcatact cgagaagatt gtatttcgac agctgatctt cagtaatagc agttgtctca
     1381 gagtgaattt tatgcacagc aagaatatca ctgatatatt cttcaatata ttgactatgt
     1441 cgaagactta agtcgctgat aatcagtatt tttttatcag aagtataatg gtgaatttca
     1501 ggattaccga taagcatcca aaagatatag ctagataaat aataaatcag attctgattc
     1561 agtagagtgg aataaaattg aagttctgca cgaaatccag catatagacg tatctggcgg
     1621 tgttgcatct gctgtgcaaa gtaagttgca cggatcgtca aatgagtcgt atcgtatggg
     1681 aaaatacggg agaaaaaata aatttgcgtt aataattgaa caacatattc ttcgtttttt
     1741 tctttgcaat cagcggatga aaacgaacag taggacatat tttttcgtat ctgctcactg
     1801 atttcatttt tttctttctc cgaaaattct gaaaaaatcg tattgacaaa atagttcact
     1861 acttttatat agctgctgta cttcaatttt cctaagagat ttttcaagat taagaagtct
     1921 tttttatcga tatttatcaa ctgtacataa gagtctccag ctttccattc aaatggagtt
     1981 ccttgggatt ctctcatgag atgcacaaca aaatacataa taaagaaaga ttgctcatat
     2041 gggctttctt cctgaaacaa attcatctga caattattct tgaccataat ttctttgatc
     2101 ttcttttttt cgattggaaa tggcaacgtg cgtcttccgt agtaacttaa aaagaaaaag
     2161 gtaacaaaga tacgcaactg gagctcactt ttaaggctag ttaactgaaa accttggtca
     2221 acgatcactt ttaataaata gctttctaaa atttgattgc attgtttcaa cttacgtgta
     2281 aaagaagacc ggctgatgga aattttttca caataatact caatcgtttt acccggattg
     2341 acaataatct catttaataa acgactgttt gtagaacttt gcgacatttc catcagaaca
     2401 tttaaataag ctggtacatt caaatgttca agacgatacc ccaatttttg tgaagattga
     2461 atcgttacat ccgttcccca gcggattttg atctcttcga tataccttac aaatgtagat
     2521 tctgaactac caattcgttt ggcaagttct tttttagtca tccacttgtc agaaatggac
     2581 agaattcgaa tgattttttc catctttcta tagtttaatt ccaacaacct gatcatatgt
     2641 ttttctccct caaccctttt ctttcaatga aacgctcatt aatcgaagga atcaacttca
     2701 taaatgaaac caattggtat gaaaattggt gtgattccct ttagcgccct tcgtataata
     2761 attatcatat taaaataaat aaaattatgt aacgcctgtt tattttaata attttcacta
     2821 ttcacgtcaa aacagaaaaa aagaaaaagg aggggaaaaa tgacgaccac agggaagaaa
     2881 ctgaaagtta ttttcatgct gataatattg agtttatcaa actttgtgcc attatctgca
     2941 atagcagaca ctacagatga tccaacagtt ttagaaacaa tttcagctga agtcatttcg
     3001 gatcagtctg gaaaaaaagc actgaacatc aagctaaatg cgaataacac cagtgctgaa
     3061 aagatagaaa aagaaattgg tctagtcgaa aattacttaa gtgatgtgga aagaaaagaa
     3121 ggagatggct atgcttatca ggtaaatagc gggaaaatta cgttggaaat ctcatcaaac
     3181 actaaacaaa ctatcgatct gagttttcca atcgatccag cactttacca cagccaggca
     3241 aacaagctga tcgtcgataa taaagaatat gacattattg atgagacaga aaataagaaa
     3301 gatacagatg tgtcagtacc aaagccagac gaaatagaag aagaatcatc aaaagaaaac
     3361 gaaaattctg tcagcccatt tacattgcct acattatcct tgccagctgt gagtgtgcca
     3421 tctaatcaaa cgattcctac agaatataca acagatgatc agggcactta tcctaaagcc
     3481 agttggcaac ctacaggaaa tacaaatgtt cttgatcatc aaggcaataa aaacggaaca
     3541 aatcaatggg atggtataaa ttcttggaat ggagatccta atgatcggac ccattcgtat
     3601 atcgaatatg gaggaaccgg taatcaagca gactatgcga tacgaaagta tgcaaaggaa
     3661 acaagtacac ccggattgtt tgatgtttat ttgaatgctc gtggaaatgt acaaaaagat
     3721 atcacgcctc ttgatctcgt attggtcgta gactggtcag gaagtatgaa cgacaataat
     3781 cggatcggtg aagtaaagat tggtgtcgat cgttttgtcg atactttagc agatagcggt
     3841 atcacagaca aaatcaatat gggatatgtc ggctactcaa gcgaaggata tagctacagt
     3901 aacggtgcag tacagatggg ttcatttgat tcagtgaaaa atcaagtaaa atccattaca
     3961 ccttcacgga caaatggtgg tacttttaca caaaaagcac taagagatgc aggaagcatg
     4021 ctatccgttc caaatggaca taaaaaagtg atcgttttgc tgacggatgg tgtaccaaca
     4081 ttttcctata aagtacagcg ggtacacgca caatcaagca gcaattatta cggaactcag
     4141 ttttctaata cgcaagatcg gccgggaaat acttctctaa tctcaagaat ctatgatgca
     4201 cctgaccaaa acaatctatc cagaagaatc gacagtacgt ttatcgcaac catcggagaa
     4261 gcgatggcac tcaaagaacg aggaatcgaa atacatggtc ttggcatcca acttcaaagc
     4321 gatccggcag ctggtctctc aaaagcagaa gtagagtctc gtatgcgaca aatggtttca
     4381 tcagatgaaa aaggcgatct ttactatgaa tcagctgatc atgcaacaga tatctctgaa
     4441 tacctagcca aaaaagctgt acagatctca gcaactgtaa gcaatggaca aataaatgat
     4501 ccaatcgcag aaccattcat ttatcagcct ggtacacttt cagtcaagag tgtggggaca
     4561 agtcctacaa cggtcactcc atctatttcc atagaaggaa ataccatcaa gagcaatcag
     4621 atctatttag gaaaagacca agaaatccaa atccattacc aagtgagaat ccaaacagaa
     4681 aatgaggact tccatccaaa tttctggtat caaatgaacg gcaggacaac tttccagcca
     4741 aacattgata ccaatgaatt agctgaattc ggtataccat ctgctaaagc tcccggagtc
     4801 agtcttcaca tcaaaaagtt atgggaagaa tttgacaaca atctagctga tcgtccagat
     4861 caagttactt ttgagattca acgggaacat acgacaaatg ctgcagcttg gaaaaacgga
     4921 tatattcgaa tcattaaacc agctaaagat acaacaaata cgtgggaacg tgcagacatt
     4981 gacaaattat ctgcaaatag cggagaaagt tatcaagaga tattatcact acctcaatac
     5041 aataatcaag gtcaagcatt cagttaccaa acaatcaaag aattacctgt accaggatac
     5101 gattctcaac aaatagatgc aatgacatgg aaaaatacta aacaattcac accgttaaac
     5161 ttgaaaataa cgaaaaattc ctctacaggt gaaaaggatc ttattggcgc tgttttcaaa
     5221 ttaacaggag attctattga tactttacta acagatcatg gcgacggaac ctattctctt
     5281 ccagaaaatg tcaaattgca aaaagaaatg acctatacgc tgacagaaac aaaagctcca
     5341 gaagggcatg gattaagcaa aaagactact tgggaaatca agatcgcttc tgatggtacg
     5401 gtaaccattg atggaaaaac agtcactact tccgatgata cgatccagtt gactattgaa
     5461 aatccttttg ttgaagttcc tgtagcagta cgtaagtatg cgatgcaagg gacggacaaa
     5521 gagataaatc ttaaaggagc agcattttcc ctacagaaaa aagaagcaaa tggtacttat
     5581 cagccaattg acagccaaac aacgaatgaa aaaggtcttg ccagttttga ttcactcaca
     5641 cctggtaaat atcgagtcgt tgaaacagct ggtcctgccg gatatgatac ttcgccggga
     5701 aattatgaat tccaaatcga taaatatgga aaaatcattt acacgggaaa aaataccgag
     5761 atgacaaata atgtatggac gctcactcat caaaatcgac taaaagcgtt tgatctaacg
     5821 gtacacaaaa aagaagacaa cggacagaca ttaaaaggag caaaattcag actgcaggga
     5881 ccagaaatgg acttagaatc gccaaaagat ggacaagaaa cagatacctt tctattcgaa
     5941 aatttaaaac ctggaactta tacgctgacc gaaactttta caccagaagg ataccaaggt
     6001 ctaaaagagc cagttactat agttatacac gaagatgggt caattcaagt ggatggacaa
     6061 gatcatgaat ctgttctgtc accaggagcc aaaaacaacc agatttcttt agacatcacg
     6121 aatcaggcaa aagtaccatt acctgaaacg ggaggaattg gccgtttagg aatctatcta
     6181 gtagggatga ttggttgtgc gttttctatt tggtatcttt ttttgaaaaa agaaagaggg
     6241 ggcagctaaa tatgaaaaaa cttggttggc ttagtatgtg tctcttcttg ttactattta
     6301 aaccagcttt tactcaggta gcaacagaaa cagaaacaga aatggttcag attactttac
     6361 acaaattgct tttcccaaac gggcaactgc cgaaaaatca tccaaatgac ggacaagaaa
     6421 aagctttatt acaaacgtat cgaggattaa atggtgtcac attccaagtt tatgatgtca
     6481 cagattcttt ttaccatcta cgggaaaagg gcaaaacggt agaagaagca caagcagaga
     6541 tcgcaaaaaa cggtgcgtct tccggtatgt ttaccgcaga agcaacaact acaactctta
     6601 acaacgaaga tggtatcgct tctttttctc tggccgctaa agatcaagaa aaaagagata
     6661 aagcgtatct tttcattgaa tccaaagtac cagaagtcgt caaagaaaag gcagagaata
     6721 tggtagttgt tcttcctgta catggacaaa acaatcaaaa actttcaact atccatttgt
     6781 atcctaaaaa tgaagaaaac gactaccctg atccaccttt tgagaaggta ttagaagagc
     6841 ctagaaatga ttttacgatt ggtgaaaaaa tcacttattc cttgcatacg acaattcctg
     6901 taaatatcct tgactatcaa aagttcgaat tgtcagatag tgcggatgaa gcattaacgt
     6961 ttttacctaa tagtttaacg atttcatcga atggagaaaa gctgacagaa ggctttgtca
     7021 tacacaagaa acctcacgga tttgatgttt tattttcgat cccttcgttg gaaaaatatg
     7081 ctggaaaaaa actgaccatt tcttatcaga tgcagctaag cagtacagca caggcgaaca
     7141 aggaaatcaa caacaacgga acactggatt ttggttttgg tgtcagtaca aagaaagtct
     7201 ctgtatatac agggagtaag caatttgtca aaatcgagac aaataaacca gataaacgat
     7261 tagctggcgc agtattcctt attaaaaaca aagcaggaaa ttacctccag caaacagcca
     7321 acggatacaa gtggacaaag aacgaatcag atgcgcttca cctgatttcc gataaaaatg
     7381 gcgctttttc aatttccggg ttgaaaacag gaagttatcg attaaaagag atcgaagcac
     7441 cttctggtta tattttaagt gaaacagaaa ttccgtttac catttcaact tttctttctg
     7501 aggataaaga ggcggacagt atattgaaag tagtcaataa aaaagaaaat agccgtccat
     7561 ttcttccaaa aacaaacgaa acgaaaaata cacttttagg cgttgttggt atggtattcg
     7621 caagctttgc aatctggttg tttatcaaaa aaagaacagg agtgaaaaaa tgaaaaatca
     7681 taaaaaaata aacgttatgt taggagtcct tttccttatt ttaccattac tcacaaacag
     7741 cttcggcgca aaaaaagtgt ttgcagagga gacagcagct caagtcatcc ttcataaaaa
     7801 gaaaatgact gatttacccg atcctttaat ccaaaacagc gggaaagaaa tgagcgaatt
     7861 cgatcaatac caaggattag ccgatatttc attttcagtt tataacgtca ctcaagaatt
     7921 ttatgcgcaa cgagataaag gagcgtccgt ggatgcagca aaacaagcag tccagtcttt
     7981 gactcctggt acaccagttg cttcaggaac gacagatgct gatggaaatg tcactttatc
     8041 tttacctaaa aaacaaaatg ggaaagatgc agtctacacg atcaaagaag aaccaaaaga
     8101 cggagtgtca gctgccgcaa acatggtttt agctttccct gtatatgaga tgatcaaaca
     8161 agcagatggc tcttataaat acgggacaga agaactagat actatccatc tctaccctaa
     8221 aaatacagtc ggtaatgatg gaacgttgaa agttacaaaa atcggtactg ccgaaaacga
     8281 agcactaaat ggagcagaat ttattatttc taaagaagaa ggaacaccaa gcgtcaaaaa
     8341 atacatccaa agtgtcacag atggattgta cacttggaca actgatcaaa ccaaagccaa
     8401 acatttcatt actggtcatt cttatgacat cggcaacaat gactttgccg aggcatctat
     8461 tgaaaaaggc cagttgatcg ttaatcattt agaagttgga aaatataatt tagaagaagt
     8521 aaaagctcct gataatgcgg aaatgattga aaagcaaaca atcacgcctt ttgagatcct
     8581 ggcaaatagc caaacaccag tagaaaagac catcaaaaat gatacgtcta aagttgataa
     8641 aacaacacct caattgaatg gaaaagatgt cgcaatcggt gaaaaaattc aatatgagat
     8701 ttctgtcaat atcccattag gtatcgctga taaagaagga acgcaaaaca agtacacaac
     8761 attcaaactt atcgatactc atgacgctgc tttaacattt gataatgatt cttcaggaac
     8821 gtatgcttat gccttatatg atggaaataa agaaatcgac ccagtaaatt attctgtcac
     8881 tgagcaaaca gacggattca cggtttcagt tgatccgaat tatattcctt cattaactcc
     8941 tggcggtaca ttgaaattcg tttactatat gcatttgaac gaaaaagcag atccaaccaa
     9001 aggattttct aaccaagcaa atgtcgataa cgggcataca aatgatcaaa caccaccgtc
     9061 agtcgatgtc gttactgggg gcaaacgatt tgttaaagta gatggtgacg ttacatcaga
     9121 ccaaacactt gctggagcag aattcgtcgt tcgtgatcaa gatagtgaca cagcgaaata
     9181 tttatcgatc gacccatcca caaaagccgt cagctgggta tcggcgaaag aatcagcaac
     9241 ggtttttaca accacaagta acggtttaat cgatgtgaca ggtctaaaat atggcacgta
     9301 ctatctggaa gaaacgaaag cgccagaaaa atatgttcca ttaacaaacc gtgtagcatt
     9361 tactatcgat gaacaatctt atgtaacagc aggacagttg atttctcctg aaaaaatacc
     9421 aaataaacac aaaggtacac ttccttcaac aggcggtaag ggaatctatg tgtatatcgg
     9481 tgcaggagta gtccttctac tgattgctgg actgtacttt gctagacgca agcacagtca
     9541 gatttagtct ttttgcaaga attggaaaaa ggagcagcgg attacgcaaa tgctcctttt
     9601 tcttttatta tcaaacagtc atcacaagga gaacacaaat gaaaccaaag aaaaggaaac
     9661 ttctagatct gctgatgctt ttcttgcttc tctcaggaat agggatacta gcttatccat
     9721 ttgttagcga cgcgctgaat aattatctgg atcagcagat catctctcat tatcagcaac
     9781 aagcggtcaa agaaaatgaa gaagtcatgg ctaaaatcca agaaaacatg acaaagaaaa
     9841 accagcaatt agcaaaagaa ggtggaaatc caggtgcaga tccctttact aagaaaaaag
     9901 aacctgcgaa aaaggataga acctattttg agaaacatac gatcggtatt ttgacgatcc
     9961 ctgagatcaa tgtccatttg ccaatttttg atgaaacgaa tactgtgctg ctagaaaaag
    10021 gtgcctcatt attggaagga acgtcttatc ctacgggggg agaaaataca catgccgtta
    10081 tttccagcca tcgaggactg cctcaagcaa agttgttcac agacttaccg aaactgactt
    10141 taggtgattc tttctatatc gaaatcaatg gacggacact cgcttatcaa gtcgatcaga
    10201 tcaagacggt agaaccgact gatacaaaag atctgagaat cgaaaaaggg caggatttcg
    10261 tgacactcct aacttgtaca ccttatatgg tcaacagtca tcgcttactt gtcagagggc
    10321 accgggttcc ttatcatgca aaagaagttc aaaaagaagt acagaaagta tccgagaaca
    10381 aacgattttt cctgtatgga ttgttagctg catgtatact gattttcctc cttcttatat
    10441 atctttttcg tcaatcgttc aaaataagaa acagaagtta gctattcctc atttctataa
    10501 tatatctatt cgaatcgttc cgtaaacaaa caggctgagg tttgaaaaag aaaacttcag
    10561 cctgttttta tgatgatatc aaagagagcc tatcaaacac tatctttctt attactaaat
    10621 gaggatcttt tcttaagttt tccgatattc tttaagaagg cctttttgca tcaattcttg
    10681 tttgtttttc aagttgcgga tattctcttc aaaattttga gaatatatca ctgagaataa
    10741 cgtatttaat acgtaatcga aggcaatttg agaggagaaa gtcgctactt tagcaaaatc
    10801 atattcctcc tgcgatataa caagacacat atcagcctgt ttggccattt ctgaatgctg
    10861 atttccggta atcagcaagg taggggcctc tacatgcttc aaataggtca ttattcgttc
    10921 ataatgatgg atgcgaccgc tgtagctaat aaaaatggcg caatcctcgc tagtcaaatt
    10981 ggctgcattc caagaagaat cactgtattc ttcagccatg atcaaaaact tattcaactt
    11041 gaccatttta ttctggaatt tccttgccgt gatttgagaa tcaccttttg caaaaagaaa
    11101 gattcgctgt gccttgttca atactttacc gatttgatct aaatcttcat gttcaatctg
    11161 caattgtgct ttttttattg actggatcga taaatccgcc attttctttg caatttgctg
    11221 agtgctatct tcacttgaaa aaggaaaatt aggatcaatc gtttgattga actgagcaat
    11281 caattgatga acttcccgcc ccagctcgaa acggaattct ttaaatccgc tgaatcccat
    11341 ttttttgcat aaacgaatga tagctgaatg ggaagtatac gtagcttttg ctaattcttc
    11401 aatcgtcatg tagactgcag cttcgatatt cctacggatg taatcgacaa tccgtttctc
    11461 tgtcgtggta aacaactctt gttttgttag tttttcttca ataagcatga tctgacctcc
    11521 aaattctatc ttactgataa acacttggtt ccgctactat ctttttgatt tttatcctta
    11581 ctttgaacat taatttcacc aaaccgaatg ataacaagtg tttttctgaa cttttttacc
    11641 agaatagcta ttataaatac tttctgcgct tttctaggat atactgaaaa catcaataaa
    11701 aggaggcacc actcatgctt acaatcgctt atcttggcaa tggaaagagt acaaatcgct
    11761 atcatttgcc tttttcaaga aaacttacga acaaaatcaa tatcaaaaaa attttttccc
    11821 catctgaacc agtgtggaaa acattcgaag acatcgaata tactgatcag ctgtctgatc
    11881 tttgggatga tcctgagatc caattagttg tcatcactac acctagtcaa tttcattatg
    11941 aatatggaaa aatggcactc gaacatggta agaatgtact agtagagaaa ccttttgcag
    12001 aaacgtcaaa ggaagcagaa gagttatttg cattagcaaa agaaaaaggt ctatttatcc
    12061 aatgttacca aaacagacgg ttcgattctg acttcttgac agtccaaaaa gtaatcgaaa
    12121 gcggaaaatt aggggatatt ttagaagtag aaatgcatta tgattatttc agaccggaag
    12181 taccagaaaa tacgcatgaa ttttctactg cgaacagcta tttatatgga catgcttgtc
    12241 atacagtaga tcaagttctt tcttatttcg gcgaaccaga tgatatcagc tatgatgtac
    12301 gtcaactttt agggaaagga cgaatgaatg attacttcga tctggatttt ttctatggaa
    12361 atatgaaaat ttcgataaaa tccagttatt tccgcatcaa agaacgtcca agtttcgtcg
    12421 tttacggaaa aaagggatgt tcgtgaaaca aacaaaagat cgacaagaag aacatctgaa
    12481 aatgttttac atgcctgatc atgaagactt tggtatcgat ttgcccgaac attatggtgt
    12541 attgacctat atagatgaac aagggagtta tcatgaagaa aaagtaattt ccgaggttgg
    12601 tgattacagc cgtgtctacg aagggattta cgagacatta gttaatggcg cagaaaaagt
    12661 catcaaagat gaagaaacaa tcttacaaat gaaaattttg gaagaaggaa tcaaaagcat
    12721 tcaagaatca gaaggaaaaa tatgacatat ttgtaagaat cgggtttgtt ttttgacgca
    12781 taaactgtct tattgtacga tgaaaaagta tagaactact aggaggaacc aacatgacaa
    12841 attatgacgg tgaaaaacac aaacgattaa gaggttcaat ggcccctaaa acagaagaag
    12901 aagtggcttt agcaggaact aattctggtg aaacagaaga caaagaagaa tcattagctg
    12961 gcacaaactc tggtgaaaca gaaaataaag aaacagcttt atctggttca aattctgtag
    13021 aagaggaaac aaagcctaat gagcccaatc actttgaaaa agatatcgaa aaagcggttg
    13081 aagatgtcaa agaaaaattt gaagaactga cctcaaaaaa taaacatgat catcattgat
    13141 cggacgaaat caattcataa aagcaggaga catttggtca cctgcttttt ttctgtatcc
    13201 attcgataga aatcgttcgg tacagtcgta aaaaaaatcc aactgtaagt tgtaccaaac
    13261 acccctcgat ttaaaagcgc taccatacta gaatataagt aggaggtgaa cagcgctgtg
    13321 aactacaaaa ttagttatct cctgaaaaca ccaagagaaa tcagtctaga aaaagcagca
    13381 gagatcatcg aagtaaatac aagcgaggcg atcaaacaat taactccttt tgccgaatat
    13441 gtggatgata cccgtatccg tataaagaaa ttccgagcaa tcgactgggc agaaaaattt
    13501 ttggatatta ctcgatttga agctgtgtat accgaacaag agcgaagaga tcttctttat
    13561 ttgatggttt attcgactgc aacgtctgtt tctgtttttc attttcaaga ttttcttcag
    13621 gtttctaaag ggacagtcct gtcagatatc aaaaagataa gaaatgaatt agaagtggaa
    13681 gattgtcagc tgtcgtatac taggaaaact ggttttaggc tgctagggtc agaagcaata
    13741 ttaagaagag aagcaaaaaa tcgcactgct cgtttgatgc agtcacttgc tggaagattt
    13801 gggctcacgt atttaaccgc aaccctttcc attgattatt acgccaaagt tagagatttg
    13861 ctagataaaa cgatttcact atcagatctt cgattcgcac caaatcgaat ggatgaatta
    13921 gcttattatt tgagtttgac aaccattcga attggaaaaa catccatgga actggtaaaa
    13981 gatgctgaac tgttggcttc tcttgcagtc actgacatta gtcgacaagt attgactggc
    14041 cttggctatt ccgaacatca aaattcggag attatatttt tctctctttg cttagcatcg
    14101 atcgttgaag gagatgtcca ggagcctgca cttgattttt tgctggaatg ttcttcagaa
    14161 gtcatccatc gcatggaatt cctgatggcc gtagaatttg cttcgtttcg tgaattattg
    14221 atgaatgtct tttatcactt ggtccctgcc tattttcgta tttcctattc tttttattta
    14281 cctaatgtga tgatcgatca aattaagcat caatatgctt ccatctacga aatgacaaga
    14341 aaagcattac ttccgttaga aagacgaata gggaagagca tccctgaaga agagattggt
    14401 tttttcacta tactatttgg cggagagatc agaaaagcgg acgcagaaga aagaaataga
    14461 aaaataagag ccgtgatcgt ctgtccaagt ggcattagct cttcattgat cttaaaaagt
    14521 gaacttcagc aattattccc gatgatcttt tttacagaga ctaattcagc tgaccgctta
    14581 cacgaggttg acgaaacttc ttacgatatt attttttcta ctgtacctct cacacttact
    14641 gataagaaaa agtctctcta tgttctgcat ccaatcatga ctgacttgga aaaaaatcag
    14701 ctgatcaatc aagtacaaag agattggctg ataccaggat tttcaatgcc ttctgcccaa
    14761 gaattgttgt cggcgctact gccttatatc gaattaaaag aaggcgtaga cgaagaaaag
    14821 ctctatcaag tattaaatcg taaaatgaac aaacttttag aaaagaagga ggatcaacgt
    14881 cctatgctat ctgaattatt aacagaagaa atgatccaat tatcggatca accaaaaaac
    14941 tggcaagaag caatcacatt tgccgcacag ccgctattat cagaaaataa aatagaagaa
    15001 agttatatca aagctatgat cgagcgcgtg gagcagtatg gtgcgtttat ccatattggc
    15061 gatcacatcg cattacctca tgctcgtcca gaagatggcg taaacgaagt agggatgtct
    15121 ttgctgaaac ttcaacaacc tgtggatctg cttgataatc cagcccatcc cattagtttg
    15181 ttcatctgtt tagctgctat cgataatgag gcacatcttc gggctttggc aaatttaaca
    15241 aagctacttt ctaataaaca aaatcttcaa gatttattac acgcatcaac aaaagaagaa
    15301 atattagcga tcataagaaa aggagacgaa tgatatgaaa atttttgcag tatgtcaaag
    15361 tggattagga acaagtttta tggtagagat gaacatcaaa caagcacttg cagatcttgg
    15421 agcagatgtg gacgagtttg aagtaagtca tacggatgtt ggaagtgtat ctagcgatat
    15481 ggctgattat ttcttcattg agcattcttt gaaagatacg ctaacttcta tacctgatga
    15541 aaagctcgta cctttgcaat cgatcattga cagcgaagaa gtaaaagaaa aagtaacgaa
    15601 gatcttgtca aaagaataaa aatgattcaa gggaggaact atcaatggat aaattgttga
    15661 atttgtttgt gtcaatcgtc agtcagccct ccatcctcgt ttctttgatt gcactgcttg
    15721 gtctcgttct tcagaagaag aaattttcag aagtgatcca aggaactatc aaaacatttg
    15781 tcggattcct agtactgatc ggcggtgctg gtttaatcag taattcacta accccttttg
    15841 cttcgatgtt ccaaacagct ctgcatactc aaggagtagt tcctagcaat gaagcagtag
    15901 tggcgattgc tttgcaacaa tacggtacac ctactgcttt gatcatgcta gtagggatga
    15961 tcgtgaatat tttacttgct agattcacaa gatataaatt catttttctt acaggtcaag
    16021 caatgcttta tgtttcttgt ctaacagcag tcattttagt aagcgcgggg tttactgcaa
    16081 atttgtggat gatcttatta ggcggggtct tcgaaggaac actattaact attacgcctg
    16141 ctctttgtca accgtttatg aagaacatca ctggaaacaa tcaagttgcg ttagggcata
    16201 ccggaaatat cggttatgct tctagtggat ggatcggaaa gttttttgga aacaaagacc
    16261 gttctacaga ggacataaag attcctaaaa gtttcagctt tttgcgagat tctactgtat
    16321 cgatcatgct cttgatgtcg atcgtatatg tcgcacttgc tttgttagct ggtcctggat
    16381 ttgttgaaag agaactaagc aatggagaca acgctgtaat ctttgcactg atccaagctg
    16441 gaacattcac agctgggttt gttatcgtcc ttcaaggggt tcgaatggtg ttggcagaaa
    16501 tcgttccagc attccaaggg attgctaaaa aactcgtacc cgattctaaa ccagcattag
    16561 atgttcctat cgtttttcca tatgcaccta atgctgtatt aattggcttt tttgttagct
    16621 tctttgtagg aacactatcc atgtttgcta tgataggctt gcaaactaca gttatcattc
    16681 ctggggtagt aggacatttc ttctgcggag ctgcttctgg tgtcttcggc aactcgactg
    16741 gcggtagaag aggggcggta ttaggttctg ctttcaatag cttattgatt agttggctgc
    16801 cattatttat ccttccagta ttaggagatc ttaaaatcgc ttcttctaca tttgccgata
    16861 ctgattatct agtaccagga cttgtacttg ggaaactagg agaatacggt caaatagcct
    16921 tgacagttgg aatcatcgct ttctttttac ttgtcgtatt atctagcttt ctttttgccg
    16981 gaaaaagaac aaacattgtt tctaaagaac agaattaagc aaacttcaaa ataaaaattg
    17041 cttggacaat atcagaagaa gatttaaagt cttctgatat gtcgcaagca gttttttgtt
    17101 ttatgcattt atttttgaat agcttgttaa gacaagtggc tgttttgcta gaagaggata
    17161 acttttacca ttcattttca ctgttccgcc tgttctatca gaagtaatca cgatcttatc
    17221 ttttgttttt tcaagcgaaa tctttccatc tttgataggt agcgtacaat tcagtgtttc
    17281 aaatgaatct agattaggtt gtatcataaa ttcttcataa cctggctttg ttggatacaa
    17341 accgataaaa tagcgtccta aaagatagat tgggcttgct ccccatgcat ggcataagct
    17401 ttttccataa ggatctccat acatactgta cttttcattt cctgtttgag tagggtcgta
    17461 ttcttcccag aaagttaccg catcatgaga taacatccct ccccaataat caagaatcgt
    17521 ttggtagacg atattcgtct gtccgagttt gcataaagca tcttgttcat agaatttgaa
    17581 atatggagta gtaatctgtg tgatagtgtc attcaataag acattcttta agatcgattg
    17641 ctgttggtct ttgttagcaa aatcgaataa aacagcgaaa atattcgcat gacgcgtcac
    17701 atttcttctc ccagattcat agcaatcgat aaaagcgcca agttcttcat cccaaaagta
    17761 gtcaaatagg ttttgtttca atagttgata tttttcttga tagagatgag tatgataatt
    17821 ttcgttctct ccatatgttt cagctaagat atctccgcaa gtgatcatgg aatgataggc
    17881 tttcagtaat aagacttgtt ctgcagcaag cgttccttct ttatccattt cagaccaatc
    17941 aataaatatc caatcttttg ctctcccata aataaaccct aattcattcg tttgctccat
    18001 caagtatctc attaatgatt gcatcttagg gtagacgatg cgtaaaaatt catagtcttt
    18061 cgtcatcata tagtggtttt cgataccaat caaccacaag atcgaatagt caacgatagt
    18121 attcatatgc tgcttgattt cattttgtcc tcttaatgct aatatcgttc gtttattgat
    18181 ttcctcgtca aaaaaaagat attgattgat catgaatgat tgatacgcat ctccagacca
    18241 aatccagcga tctcgtttga taccgtcgat aaaaaacaaa tcactacata acttgaaggc
    18301 ttcttctgat actgaccaga tctgattcag taaagaagaa tcgcttgaaa aagcagcttg
    18361 tttcgaaaga gataaaaatt catgaaaaac ttgaagttga accattccag tagaaacctc
    18421 tggtataaac aaatagcgaa aagcgcgttt acgagctttc atttttcttg tcattcctat
    18481 ttgcttatag tagcaataat cgatatccat agcttctaca tcggactctc cataacatag
    18541 agtgatagat tcgatatggt catccagaga tattagttct acacagccat tcactgctcg
    18601 cccaaaatcg aataagattc ctccgccaat ctccgtttca gtgactggat ggacgcattc
    18661 tttttgataa tagatttgat ttggatcttt tgcgatatcg acataaagcg gactccagcc
    18721 agctggatat tcttcaataa aattacttgc tgtccagcca atatctgatt ttacttcgtc
    18781 tccctcgata aacatcgctg gtagccctga tgtatgtcct acaaagatac gaatttttgc
    18841 tttaccagct ggacaagtca gccattttcc acatggatgt ttttgtccat taatttctac
    18901 atacccaact ccttggatcg tcactttaaa catggaaggt tgatcaagaa agtaatatct
    18961 cttaaacttg acgttccgat ggcaatcatc catgtaccag tacgctggcc agtcaaaacc
    19021 tcgttcttcc cgttggaaat tttgtagcag tccatggcgg atctcaaagt ctccaggata
    19081 ccaaagccat ctatctttat gtttttcatt tgtcatttta cacgacctcc atcaaattta
    19141 ttttgttgtc aattgttctt ttatttcttc atctactctt atcactgtct ttctattcgt
    19201 cctgtgtact actagaaggc taataagcgc gctaatcaac agcaagatac tgccattgac
    19261 gaaaacagca agtgttttac tcccaagaga agcaaaagag agaaagagca aaccatatgg
    19321 tgcaatgaaa gtacctaaat tagcgccaac caataagatt gaagtattga aactctccgc
    19381 gtacttgggc gcttggatac ttacttggtt gaataagtaa ggaatgaata acgaaaaaga
    19441 aattcctgtt aggatcgcac caaacccaat cacatacagg tttatcacag tggcaatcaa
    19501 tacaaagccg attcccatca gtagtaaaga aagtgtaagc atatagcgat gtgcttttgt
    19561 ctgaagccag ccaaaacaaa ctccggcagc catacttgcc aattgcatga ttgttaagat
    19621 attcgctgca ttagtttcgt ttcctagaga ataagtcgtc aggactgtgg ctaatttaat
    19681 cgatacagca ttatatacca cgatgaccag caagagaaaa ataccataac taatcatttt
    19741 aggattcgtt ttgattactt gtcttgttgg ggctgtgcca tgcgattgaa gttcagttgt
    19801 ttcaggtgta ggaacaaaca aaacaaacaa gacaagaata ggtaagctga tggcgtagac
    19861 taaaaaggta tattgccaac cgaactgtac tagctgtcct gcaacaaatg tcattaaagc
    19921 tgcaccaaat ccttgaaatg cactttgaaa accaatcata gaggctcgtt catctccgtg
    19981 ataaaaggca gcaatcatgc tgactgccaa agaattgatc aatccaaagc caatacctag
    20041 tccagctctt gaagctaaga caatactgta actttgtgag aagaggggga ggataccaca
    20101 aatcgatgca atcactaatc cgatgatcac agtttgtttt tgtccgattt tcctagcaat
    20161 aaaactacta atcaaaacaa aaatagtgac cataaaagag ggagcagtcg ttaataattc
    20221 aacagcagat aaagaacgcg tggcataagt agctgacata ctgggaattg cagacgtgac
    20281 tgcaccagca gaagtcaaaa taagagagac tgacagcaga gaaaatttca tcatcttttt
    20341 attcataagc atgttccttt catttttgta agcgcttatc aaaaatatag tatgaagtgg
    20401 ttattctctc aatagcggaa tcacaggaag ttcatgtccc aaccatagaa agtctttatg
    20461 aaaccaaaaa tagctttgca tataatagca gtacagcgtg aactaataag cgaaagaaac
    20521 cgcttacaaa aaggagggag tcaattggaa ctagctgaat tagacatttt tttgcgtaac
    20581 gaagaatcaa tcgaaactct gcagcgtgaa caaggaatga atatcaatga tttaaactta
    20641 gcttaccatc ttcagacacc gccaaaaatc caccgagggg atctatttca acagggaaat
    20701 attcatatca gtaaacaccg tcgatatgcg gaagttcccg agcatacaca tgattttgtt
    20761 gaaatcaatt atatgtatag cggtacatgt ttacaaatta ttaatggaga aaggatagag
    20821 atggaagaaa gtgacgtggt gatcattgat aaagaagcta ctcaaagaat cgaatacaca
    20881 gggcaagagg atattttaat caacatctta ttaaaagaag aaaccttatc tacagagata
    20941 ttgaatcatt tggcaagcac aaataatcta ctttcttctt ttttgataaa tgcatcaaaa
    21001 gaaaaagggg aacatacaca attcattcat tttcgcggcg gaaaaacaag tcgtttgacg
    21061 catttgattg aaaatatgct gttgatttat tttgatacat acagtcataa gatccgttcg
    21121 ttaaatctct atacatcact attactgatc gaattgactc atttattaga acaacaaagc
    21181 ttttcatcag cagatggatg cgcagaaaat gagatgctta gagtattaga ttatatcgat
    21241 aagcatttta cttcttgtac gttaaaagaa acagcagctc attttggcta caatccggtc
    21301 tatcttagta ataaattgaa aatgtatttt gatgcttcct ttcaagaact ggttctaaag
    21361 aaaaaattcg actttgcgtt ggaactgatt caagaaacga gtttttcgtt ggaagaaatc
    21421 gctaatcgta tcggatataa acaaacgact tctttattca aattattcaa gaagtattca
    21481 acatgtactc ccaatgaatg gagaaataaa cattaagaag aaaatctttc actaaagcat
    21541 atgaaggaaa attgttttag gtgcgattgc tgtcttatta tatcgttgca gcgaattttt
    21601 ttggatttat tttcagttct aaacttttgg ttgtattatc aggggatata ggtgtaaaca
    21661 tttgtcattt atctgttatg cgtattcagg aattcgtttc catcaaaaat tataataaca
    21721 actattataa ttaacatgtt gataaaatga ataaacaaga ctatactttt catgaaagta
    21781 acaaatagtt atgaaaagga gctgtttatt catgaaaaaa acaagtcgta aagtagtaat
    21841 cgtcggtaca ggttttgttg gaacaagtat cgcttatgca atgatcaacc aaggaatctc
    21901 taatgaatta gtattgatcg atgtgaatca agaaaaagca gaaggggaag cattggacct
    21961 tttagatggt atggcttggg gcgatgaaaa tgtggctgta tggtcaggtg gctatgaaga
    22021 atgtaaagat gctgatatcg ttgttatcac agctggtatc aaccaaaaac ctggtcaatc
    22081 tcgtttagat ttagttaaaa caaatgcgtc aatcatgcgc caaatcgtaa aagaaatcat
    22141 gggatcagga ttcgacggta ttatcgttgt tgcttcaaac ccagtagata ttttgacata
    22201 tatcgcttgg aacgaatctg gtctgccaac atcacgtgtt atcggtactg gaacaacatt
    22261 ggatacaact cgtttccgta aagaaatcgc attgaaatta aaagttgacc cacgcagcgt
    22321 ccacggctat atcttaggtg aacatggtga ttcagaagtt gctgcatggt cacatacaac
    22381 tgtcggtggt aaaccagtgt ttgaaattgt agaaaaagac catcgtattg cccaagatga
    22441 attagatgtg attgctgata aagtccgtaa tgctgcttat gagatcattg atcgtaaaaa
    22501 agcaacttac tatggtattg gtatgagtac tgcacgtatc gtaaaagcta tcttgaataa
    22561 cgaacaagct gtattgcctg tatctgctta tttaactggt gaatacgacg aaaaagatat
    22621 ctttacaggt gtgccatcaa tcgttgacga aaatggtgtt cgtgaagttg ttgaattatc
    22681 aatcaacgaa gaagaaaaag caatgttcag caaatcaaca agtgctttaa gagaagtatt
    22741 gaacactgta ttataagaaa caatgaatat gaactaaaaa tcctaaaact gcttaacagc
    22801 tgaaatagct gttaagcagt tttttattga tatctaatag gtaagcaaat aaatagaatg
    22861 gttaaaatta atcggtccga aaatgtatcc gtacatatga acatatgaaa tttaatctgt
    22921 tgattacaat aagcgaatcg ttatcaatgt ttatctgtgg ttaattaaaa aatccttccg
    22981 caacggcaaa tacaatgatt catatctaat tcgttcatat ttgattaact gaataaagaa
    23041 actggttttt atcattcaat taaacgacaa aaaaagtaag aaaaactgca aaattttttt
    23101 ataattttgc ataagcagat tttgatggaa aaatgacgat tttt
//
LOCUS       DAZKOW010000041        22719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000041 DAZKOW010000000
VERSION     DAZKOW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.041"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(11..1942)
                     /locus_tag="ACG912_001497"
     CDS             complement(11..1942)
                     /locus_tag="ACG912_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFQ0512825.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG912_001498"
     CDS             complement(1960..2874)
                     /gene="pfkB"
                     /locus_tag="ACG912_001498"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFQ0512826.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2871..3623)
                     /locus_tag="ACG912_001499"
     CDS             complement(2871..3623)
                     /locus_tag="ACG912_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0512827.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3782..3991)
                     /locus_tag="ACG912_001500"
     CDS             complement(3782..3991)
                     /locus_tag="ACG912_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512828.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4002..5330)
                     /locus_tag="ACG912_001501"
     CDS             complement(4002..5330)
                     /locus_tag="ACG912_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512829.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            5466..5621
                     /locus_tag="ACG912_001502"
     CDS             5466..5621
                     /locus_tag="ACG912_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFQ0512830.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG912_001503"
     CDS             complement(5695..6282)
                     /gene="yihA"
                     /locus_tag="ACG912_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFQ0512831.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG912_001504"
     CDS             complement(6365..7615)
                     /gene="clpX"
                     /locus_tag="ACG912_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFQ0512832.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG912_001505"
     CDS             complement(7862..9151)
                     /gene="tig"
                     /locus_tag="ACG912_001505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFQ0512833.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9305..10237)
                     /locus_tag="ACG912_001506"
     CDS             complement(9305..10237)
                     /locus_tag="ACG912_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512834.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10394..11053)
                     /locus_tag="ACG912_001507"
     CDS             complement(10394..11053)
                     /locus_tag="ACG912_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0512835.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG912_001508"
     CDS             complement(11056..12081)
                     /gene="sppA"
                     /locus_tag="ACG912_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0512836.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12094..12384)
                     /locus_tag="ACG912_001509"
     CDS             complement(12094..12384)
                     /locus_tag="ACG912_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512837.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12793..14598
                     /gene="glmS"
                     /locus_tag="ACG912_001510"
     CDS             12793..14598
                     /gene="glmS"
                     /locus_tag="ACG912_001510"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFQ0512838.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG912_001511"
     CDS             complement(14670..16025)
                     /gene="glmM"
                     /locus_tag="ACG912_001511"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFQ0512839.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16049..17218)
                     /locus_tag="ACG912_001512"
     CDS             complement(16049..17218)
                     /locus_tag="ACG912_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFQ0512840.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG912_001513"
     CDS             complement(17215..18102)
                     /gene="cdaA"
                     /locus_tag="ACG912_001513"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFQ0512841.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG912_001514"
     CDS             complement(18291..21983)
                     /gene="nifJ"
                     /locus_tag="ACG912_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFQ0512842.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22182..22703
                     /locus_tag="ACG912_001515"
     CDS             22182..22703
                     /locus_tag="ACG912_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFQ0512843.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         6326 a         4932 c         4041 g         7420 t
ORIGIN      
        1 ttttttattt ctatgcttct gctactgcag gagcttttct caataaacca taaatcacac
       61 cagatacgac tgctccgata gcaataaaca ccaaatacat gaccgggtga ctcagtaaga
      121 atacaacgaa aatcccgccg tgaggagcca gtaatttgat tccgaaagct ccgactaatc
      181 cgccagttaa agcagaacca ataataaaac ttggaatggc acgaatcggg tcagctgctg
      241 caaatggaat cgatccttcc gtgacaaacg atagtcccat cacgatgttc gtcaaaccag
      301 catcttgatc tgttttgctg aatttattct taaacaaacg tgtagcaacg aaaattgcca
      361 atggaggtac cattcctcca gccataacag aagccatcac gacacttccg ccagttgcaa
      421 cagtcgtagc taaagttgct gtaccaaaca cataagcggc tttgttgatt ggaccgccta
      481 aatctgccgc catcattcca gctaataatg caccaagtaa agcagcattc gaaccactca
      541 tacttgtcaa gaaaccgttc aatgcatcat tgattgcttt catagggaca ttgaccagca
      601 acatcaagaa accagtaatc agtaagccaa acactggata aaagaggatc gttttaattc
      661 catccaatga ttttggtaag ttcttgaata atttacgtaa aaatacgatg acataaccag
      721 ctaagaatcc accaatcagt gcacctaaga atcctgcacc gcctgtatta gctaatgcac
      781 cagcagcaaa tccaacgatc aaaccagggc ggtcaccgat acttgaagca ataaatcctg
      841 ctaataccgg cagcatgaaa ccaaatgcag cttgtccgat ttggttgaac cagctagcag
      901 cttgattgta attccctaga tttgataatt gatcatgtgg cacgccaata aattggtcga
      961 tcatgaaaga aagcgcaata gcgattcctc caccaataac gaaaggtaac atgtgtgata
     1021 cgccgttcat caaatctttg taaattcttt gtccgatagt accatccgca tttccttctt
     1081 ctgaactgct ttcttttcca tcaccatgga atactggtgc agtaccacta agggctagat
     1141 tgatcaactc ttctggttta cggataccat cactgacagg acgattgatc agttcttttc
     1201 cgtcaaagcg attcatctct acttttttgt ctgctgcaac gattacacct tctgcacggg
     1261 cgatgtcttc agcagtcaat cggtttttga ttccttctga accgttcgtt tcgactttga
     1321 tctccacacc catttctttc gcttttttct tcaaagcatc ttctgccatg taagtgtgag
     1381 cgatccctgt tggacaagct gtaacggcaa caacgaattt tctttctgga tgagcagaag
     1441 cttctgcagc ttgtgtttct tgttctgctt tttcttctgc ttctttttgc tcttcagctg
     1501 tttggaacaa gtcttggact tcttctggtg tatttgcttc ttttaatttt cctacgaaat
     1561 ctgcatcaat cagcaatcga gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc
     1621 cttctggagc tgcgatcatg aaaaacagat aagttggctg tccgtctaag gcttcataat
     1681 ctactccttt ttggcttttt gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg
     1741 cgtgaggcat ggcgattcca tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa
     1801 ttccttcttt atatgtttcg atatcggaaa tccggccggc atcaaacatt ttttggacca
     1861 tttcatcgat tgcgcctttt ttatcagtcg cttgcaaatc catgatcatc gcttctttga
     1921 ccattaaatc cttgatattc atgattatcc tccttgaaac taatcaattt ttgtgatttc
     1981 aacttctggc agcaactcat caatcaattc gcgagctgcc aagtcatcag aaaaagctgt
     2041 tgcgcttccg caagcaactc cccatttaaa ggcttcgatt ggatctttcg tacggctgta
     2101 agccccaata aagccagcaa tcattgaatc ccctgcacct actgaatttt tcaatgggcg
     2161 aactaataca ttcgttttat aagttccttg ttccgtgaag agcaaagcac cgtctccagc
     2221 cattgagatc agcacattct gtgccccttc agctagtaat tttttcccat acggaatgat
     2281 ttcttctaca gattggaagg ttgtgttgta aagctcagct aattcgtgat tgtttggttt
     2341 gatcaacaac ggtttcttaa caagtgcatt ttttaggtca tcacctgttg tatcaatcac
     2401 gaaatctgca cctttttctc gaatgatatc aatcagttct tgataaaatc cttttcgcag
     2461 gctagctggc gcgcttcctg acaatacgac gatatctcct tgtcctactg ttcctaatgc
     2521 ttgcttcaac tcatcgattt cttcatctga aatagatggg cctaagccat tgatctcagt
     2581 ttcagtgtct gattttagtt tgatattgat tcttgtatct tcttttacgg ttgtgaacaa
     2641 agactggatt tgttcttttt caagccactt cgaaataaat tcgccagtga accctcctaa
     2701 aaaccctagt gctgtagatt gagaatcgat ccgtttcaaa atacgggaaa cattgatccc
     2761 ttttcctcca ggtaacttga agtcttcctt cattcggttc aaatcgccta gttttaaccc
     2821 atctacgcgt acgatataat caattgatgg attaagtgtt actgtgtaaa tcattgtatt
     2881 gcctccttaa ttgttgtctt ttcacggaat tcattaacga attctaatgg acaacgctcg
     2941 gttatgatcg tggctgcttc caaaggtgca acagaggtga aggacaactg gttgaatttg
     3001 gtatgatcaa tcaatacaaa tgtctcttcg gaatttttca tggctaaacg tttaacagct
     3061 gcctcttctg gatccggagt cgtgaatcct tgttcaagat gggcaccgtt cattcccata
     3121 aatgctttat taaaatgaaa attttttagt tgttctatcg cattagatcc taaaacagca
     3181 ttagttgcca gctttatcgt tccacctaaa ataatggtag caagctccaa atcaactaaa
     3241 tttgccgcat gttgaacgga atttgtcact actgtgatct gttttccttg cagaaaagga
     3301 atcatttcta acgtagtaga gccagcgtct aaataaatga catcttcgtc attaatggtt
     3361 tcagctgccg atttggcgat ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc
     3421 atattctgct caaaattcaa taattgctct ttcttagctc cgccatgaac tctttctaga
     3481 aacccactat tctccagctc ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa
     3541 aggtttacaa gatcttgtga tttcacgatt ttctgctgtt caagcaagtc taggattttt
     3601 tgtctgcgtt cctctgtaag cattttcacg atcacctcaa tacaaataat agcatatttt
     3661 ttttctacta gcaatataaa tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa
     3721 aaaaataaaa aaccagtcag aaagactggt tttttatttt tttgtctatc atcaagccgt
     3781 tttatcgttt tttattttcg cttcttgttt ttttcatata aacagaagca ggttttcttt
     3841 ccttatgaag aagataataa taaagtccca tcccgccaaa catgaccaaa aaaccgatga
     3901 ctgatcgaat aggaaaatta ggaacaaaaa agagacagac ggataataat gatgccggaa
     3961 ccattattcg ataatagagt tgtttatcca ttttcttcac ctcatatttt ttgtaaagta
     4021 atcaattgtt ctaattctcg gtaatctaaa aattgcacac gcagatgctg ctcacctttc
     4081 ttgacatcga attggatact tcgccgcttt ttctttattc gtttgaaggt aacatcagag
     4141 agtctatgtt tgatttcctt taacggtaat tctgacaggg attctgaagg aacatagatt
     4201 cttaaggata aaagatactc ttgatatttt tcatctgtta cctctacttc tagatattcc
     4261 ctaatataat aacaataagc ttcgattcca ttatccaaag caacagtcgg atcgataact
     4321 ttatagcaca tcatgcgtga tcgattctcg tcaacccaag gaaaatacgc ctgagcattt
     4381 agtgttccgc gaacacgcct ttctttataa tctccttgtt ggatatcata actttttgtt
     4441 acaaaaaatg aagcattctt tttaggaaca ggttgcattt gtgcattctg atactggaca
     4501 gattggatcg tatggtcagt gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca
     4561 ccattgtccc aagcaagatg atcaaaccca agctggtccc taaaatagga ttgaaacatt
     4621 tgattgtttg gcttttgcca gttaaagggg tctcgataaa gctggataca tatgacttcg
     4681 ttttctaatt ccttcacttt ttctacttgt tggatgtcga gaaaaatcgg tttagggatg
     4741 atttgatgat aatagcgact ataatacatt tcccaacacg aagtgaaaca taaaccttgg
     4801 taaaaaagat cgtatcctgg aaattggttg caatcgacta ctacttcccc gttttgttgt
     4861 ttcattttag gtaaatgttc gatttcatcc tttttctcaa acaatagccg ttgagagatc
     4921 tgttttgtat tgtgatagat aaactcgcta taagctcgca tataggcgaa caccccatga
     4981 gcaatcattt ttttctgaat gaagtctcga atcaaaaatt gcttttcttt gaaaacagag
     5041 atagacaata cattttttat caataattga ttatcttcca agcgaaaact acattcgttc
     5101 tcgtcatttc ctacaactaa ccaactttcg tctcttaccg atgctagcat tattttttct
     5161 tgaagctttc gaagatcata cggatccgct tgttgtgtga aagagcggat aaatgttggc
     5221 tggatcaaca gacgtgtcaa aatagcccaa tcgcttattt cctgtttttt ttcttcttca
     5281 tttaagtctg tgagctggat agtcatcaat aatttatctt cgccaatcat tttctcacct
     5341 ctttcaaatc tttttataat ttaattataa ccaatgataa agaaaaacta aaactttatg
     5401 tcttagaaac caatttggac gattacatga tagactaagt aaaaagaaac aaagaggtgt
     5461 ttacaatggg gttgaaattt gaacgtacag ataatttaga taaacttttc gaatcatttg
     5521 ctgtcgatcc ggaaaaaaca gttgcaccaa aagaagagat cgatcgattt ctaaaaccac
     5581 agccacaaaa tgaaaagaaa aaagacaaga aacaaaagta agttaagctc aaaaaaagac
     5641 ttaccagcca aacttttatg aaagagtttg actggtaagt cttttttatt ctttttatga
     5701 ttcaattgct tgttcgattg ctgcccacgc ttcttctttt ccctgttttg taacggaaga
     5761 aaagagaata aacgtgtcat tgcgatcaaa atccaacgct tttttgattt cgctctcatg
     5821 cttgttccat ttaccacgag ggatcttatc tgccttagtg gcaacaacaa ttacagggat
     5881 atcataatac ttcaaaaatt gatacatctg gatatcttct tgagaaggct tatgacgcaa
     5941 atctactaaa gaaacgaccg cacgtagctg gtctctagtc gttaaatatg tctcgatcat
     6001 ctttccccat tttgctcgct cagttttgga tacttttgca tacccatatc ccggaacatc
     6061 gacaaaatgt aatgcgttct cgatcaaata aaaattcaat gtctgggtct ttcctggctt
     6121 accggaagta cgggctaaat tctttcgatc aatcaatgtg ttgataaaag aagatttccc
     6181 tacatttgat cgtcctgcca gagctatttc tggcaattgt gtatctggat attgttttgg
     6241 tgccactgca cttatgataa tttcagcttg atggactttc atggtctctc tccttaacaa
     6301 ggtgatgaat aaagcaagcg gctgtcgcaa aagccaaaat gacttttacg gcagcctctt
     6361 ttcatcaacc cgcttttttc tctgaatctt tatagattac tgttggctca ccagacaatt
     6421 cagctgcttc ttttgtgata atcacttttt cgatgttttc atttgaaggg atatcaaaca
     6481 tcacatccat catgatttct tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt
     6541 cgattgcttt tttcgcaatg gctctcaacg cttcaggttc gaattcaagt tcggtattat
     6601 ctagagaaag aagtttttga tattgtttga ctagcgcatt tttcggctca gtcaagatcc
     6661 gtaccagatc atctgttgtc aatttctcta aagctgccat aacaggtaat cgtccgataa
     6721 actcagggat caatccgaat tttagtaaat cttcaggaat gatatgctgc atgacacttt
     6781 cgtcttcaga aagtttttgg ttttgtgtac caaatccaat cgtcttttcg cccatacgat
     6841 tcttcacaat cgtttcgatg ccatcaaatg caccacctac gatgaacaat atgttcgtcg
     6901 tatcaatctg aataaattct tgatgtggat gtttacgtcc gccctgaggt ggcacacttg
     6961 caacagttcc ttctagaatt ttcagcaagg cttgttggac accttcgccg gaaacgtcac
     7021 gagtaataga tacattttcg cttttacgcg caattttatc gatctcatca atatagatga
     7081 tccctttttc tgcaagttcc acattgtaat cagcagattg aagcaattta agcaaaatgt
     7141 tttctacatc ttcccccaca taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa
     7201 aaggtacatt caatgttttt gctaatgttt gtgctaaaaa tgttttacca gaccctgtag
     7261 gtccgatcaa gcagatattg cttttttgca gttcaacatc atctgtgttt tcttgtgctt
     7321 gctgattgac acgtttatag tgattgtata cggcaacagc taacgagcgt tttgcgcgat
     7381 cttgtccaat cacgtagtta ttcaatactt caagaatttc tagtggttta gggacttcag
     7441 tgaattcgcg tacagcttct tcataaaatt cttcatcaat gatttcttta cataggtcga
     7501 tacattcatt acagatgtag acccctggac cagctacgat ctttttcact tcttcttggg
     7561 ttttcccgca aaaagaacag cgtacggtct cattgctact tggattgtca tacatggtct
     7621 tcacccctta acttgcaaaa agtatgcaag aaactatcaa agactttaca gtcttttact
     7681 atcataacat agtttagtaa aaagaaaaag tgtttaacaa aggatcaact gacctatacc
     7741 tttgcatttt ttactcttaa caaaaaataa ggaactgtga caaaatcgtg tcacagtccc
     7801 ttatttcgaa taaacaattt tctagaagta gcttcttcag aaatgattta ttacttgatt
     7861 tctattcttt cgcttctgtc gctgtacctg tgatcaattc gattgctttt ttcattgtca
     7921 catcatgttc cagcatatct tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt
     7981 atgtttctga taattcttgg atttctgcat tgatttcttc ttctgttgct tgaagatttt
     8041 cagcagctgc aattgcttcg atcacaagat ttgttcttgt gcgcatttct gcttcacctt
     8101 cgaattgttt atgcaagtct tcttctgtag aaccagttaa ttgatagtac atttcaggag
     8161 agataccttg acgttgcatg ttgttcaaga attcgtccat tgaacgatgt acttcatcat
     8221 gaaccatcac gtgtggcaat tcaacgattt ctgcgttttc aacagccata cggatcgctg
     8281 cttcatcttt tgcatcgtcc gctgcttttt cttttgtttc tactaattct ttgcgatatt
     8341 tttctttcaa ttcgtctaat gttgatactt cgtcatcaac atcttttgcg aattcgtcat
     8401 ctaattcagg cacttctttt gcttttactt catggattgt cactttgaag actgcttctt
     8461 ttccagcaag atcttctgct tgataatctt ctgggaaagt tacatttaca tctagacttt
     8521 cacctgattt atgaccaact aattgatctt caaaacctgg aatgaatgag cctgaaccta
     8581 attcaagtga gtaattttcg ccttttccgc catcaaacgc ttcgccatct acaaagcctt
     8641 cgaagtcgat tactactgta tcgccatttt cagctgcagc atcttctttg ataactaatt
     8701 cagcttgtgc ttcttgttca cgtttgatac gtgcatccac gtcagcatct gttacttcac
     8761 ggtcttgttt ttcaacaact agatctttat attgtcctaa ttttacttct ggtttcactg
     8821 ttacttcagc agtgattacc cagtcttgac ctttttccat gctttcaaca tcgatttttg
     8881 gttgagaaac aggatcgatg ccagcttctt tgacagcagc ttcataagct tctggtaata
     8941 cagcattcaa tgcatcttca tataaagctt cttcaccgta catacggttg aatacttgac
     9001 gagaaacttt ccctttacgg aatccaggta cgttcaagct ttttttcact ttattaaacg
     9061 cagttgttaa tcctttttgg atttcgtctt gcgcaatcgt aaattttaaa acaccatcat
     9121 tcgtgccttt tttttcaaat gttgcagtca tttaattccc tccgagttta gaaattcatt
     9181 ttatacatca aaaacgtttc atgcatacct ttaaatagta cactaaccct ttcaaattgt
     9241 aaacatattg aatggataga atccttgttt ttcataacat ttttatcttt ttcactgata
     9301 aaatctaatg gaagaagatc atttgctgca aaattttttc tctttttttt aaaaaatctt
     9361 gtttttcatc ttctgtacct tcatacatcg tttttcctgt acacgcctct acagtcagtt
     9421 gaaaccagcg cttcgggtct tcgatgtatg tatcatagac aggataaaca attgcagatt
     9481 gcaacgtgaa ttcttctttt aacacggaac taagcagcgc atcttcattc ccgtaacgat
     9541 ctgaaaatac acataattgc tggtaacttg cctgctgctg cggctttggc agatcttttg
     9601 gtatgacttt gactaacttt tcttcaatcg tcaaatagaa aacttgatgg cattcgttca
     9661 attgcctaag tgtctccagt aattttgccc ttgctaaaat atggacctct ggcgatacaa
     9721 taagtttact cgctgtttgg ataaactcat gataaggaag ctgttcgatc tcatcaatag
     9781 acgcaagctg acgtgcaggt tccaacgaag gaagttcctc cgatttattg tggagtgcct
     9841 tgatcttctg cctctccatt tgtccggata attcttctgc aaattggatt ttttcaatga
     9901 cccgctgctc gaaagcttca taaaaatctg tacttgctat caattttcta gcatataaaa
     9961 aatcttgtgt actgattaac aagtcaaaat aaaattcagc aaactcctca ttttgaagat
    10021 actgtttttc atgcaagagt gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa
    10081 gacaaaaaac aagcaggcta ttagcctcaa agctttcttt caactcgtaa gctcgttgaa
    10141 ataattcttt tgcctgctga tattgctggt ttgctagtgc ttgttgtgcc actcgcatta
    10201 aattctcata tttttctgga aaatcaatcg gcttcacgca gtgtcctcct cttgctcata
    10261 ttcagctcat gttcaatagt agcaaaacaa aaaaagcatg acaattaaaa ataactgaag
    10321 caccctctaa aagctagatt ttagatctga cttttagaag gtgctttcaa ctgttactgc
    10381 tttttattgc tcatcagctg ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa
    10441 caacacttgt attagtgaaa aaatcgccca gatgttgctt tttctgatta aagactgctg
    10501 gaagataccc taacatcagc aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa
    10561 ctaaaacggt tgaccagcta agttcttctt catcaaagct gatgactcga ataccaaaga
    10621 tcattttccc aatcgtttgg ccatgattga atttcgtcaa taaaataaaa tacgctagat
    10681 aaataagtaa ggctgtgaat ccgtagagac tcagataaga atctgaaaaa ttaagccaac
    10741 ctagattttt ggcaatacca agtgttaaac gcgtaatagc tccgatacac aaaagatcga
    10801 ccaagaaggc aaacattcgg ataaaaaaac ctgcatagaa atacttagga aaatcatttg
    10861 cttttggcgg ttcatttctt gtgtccgtat actttttcca tttacgttgt ttctttttga
    10921 tttcttcggg cgtcaaaggc tctctttcaa aagagctcag taccttttgg gaaaagttgg
    10981 agtcaggtcc tacagcaggc tgctgttgct tatctttttc ttgttcctta ttttcatttg
    11041 aaagctctga catgcttact cacctccgta ataatacatt gcttttggtg attccgctgt
    11101 ccctagattc tcaagaacgg ataagatctg actagtctca gaaggcttca atccctggaa
    11161 ttctgctagt ttacttccta accatgtact agcaaaccca gatggcgtgg aagaatattc
    11221 tactagttcc gcatcttcaa gctgttgttc ctttcgtata gcttttaaag catcttcagg
    11281 aaatcccaat tcatcgacta aacctacttc ttttgcttgg acaccatcat aaatccttcc
    11341 atcagcaatt tttttgactt cttcttcaga tttattccgt ccctcgctga cgattgctac
    11401 aaaacgacta tatgcgctgt cgatatatgc ttgaagcacc gcttcttctt cttttgtctc
    11461 tggtcgcgtt cctgaaccca tatctttcaa cgctccgctt ttaactgttg tatcttccac
    11521 acccagtttt tctagaagtc ctgaataatt caatcctgac atgatcacac caattgagcc
    11581 ggtcactgtt tcttctgttg caaagatttt atctgcttga gcagaaatat agtagccacc
    11641 gctagctgcc atgtttttca tgctgacata cattgggata tcatgttctt tacgaatcgt
    11701 atccaatttc ttagcgattt ccgcactttc gtagacaccg ccaccaggag aatttacttc
    11761 taaaaagata cctttgactg ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt
    11821 tttatgattg taaccttcag aagcaaataa acctgaagac cctcccgcag caatcgttcc
    11881 atctactgat aattttacga ttttttgcgt actgtctccc tcttccaaca catttggagc
    11941 tagttcttca tccccatata accaactgtt caatccctca atttgatcgt tttcctgagg
    12001 cttagtcaaa ctagctgaaa ctaacgaaac cacaaataat cctgcagcaa tcagaactgc
    12061 tgcccaacgt cttttattca ttctttctcc tttctaatac aagtttttta tgacgatcac
    12121 agtttctgac tgctcagaag tgtcgaaccg ctctgctttt tcttcccgcg catacccgga
    12181 aaacgactct tttgttaatt catcttctac cgtaaactca cgagcttgag caacttccaa
    12241 ggcatcaggt gcgatttctt ggatgctatc ccagccagta tctaaaaata ttttcgtatc
    12301 atctgataca ccagctgatg cttcgaattt ggacaattgt ttttttaaca ttcccagtgt
    12361 catgatctca atttttgaat ccatttttac tcctcctcta tccgattact ttctattgta
    12421 ccgaactcct gaaaaagatg atagataaaa acccattatt ttttgacata aaaaggacat
    12481 ttgacaaaga atttgatata taccaattta ttttttattc tttttttgtt gacaagaaca
    12541 ttcgcaaaag gttatgattt taaatgtttc cagataaaaa acaaaaagga ggatagttat
    12601 accttttagg atagcgccag gtctgtgtaa acagatgacg aggagagggt ttatcgaaag
    12661 attcggcgga tggccctagg gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa
    12721 gataaaacaa aaggattttc ctgcagctgg aagcattcat acgaattcat tatagaaagt
    12781 aggatgtttt ttatgtgcgg aattgttgga atgattggat taaaaaatgt aacaccagga
    12841 ttgattgacg gtctagaaaa attagaatac cgtggttatg attcagctgg tatctttgtc
    12901 tcagatggca caactgatta tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac
    12961 aaaataaata ttgatactac aggaaatata ggtattggtc atacacgatg ggcaactcac
    13021 gggcaacctt ctgaagaaaa cgcccatcca catacttccc aaagtggacg cttcgtgctc
    13081 gttcacaacg gcgtaatcga aaactttgaa gaacttaaaa ttgcttattt agcaaatgac
    13141 cactttattg gtgagaccga tactgagatt atcgctcatc taattgaaac tttcgcgaag
    13201 gatactacaa cgcaagaggc gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct
    13261 ttcgctttga ttgatcggac agcacctgat gttatttacg tcgctaaaaa taaaagtcca
    13321 ctattaatcg gactcggcga tggattcaat gtgattgcca gcgatgcaat ggctatgctt
    13381 gctcatacga aggaattcgt tgagattgaa gatgaagaga tggttaccgt cacatcagaa
    13441 aaagttatta ttcaaaattt tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa
    13501 gtagatgctt cagatatcga gaaaggtacc tatccttttt atatgctgaa agaaatcgac
    13561 gaacagccta ttatcatgcg tcgtatcgct caaaaatacg taacagaaga caatcgtgtc
    13621 tctcttgatc aaaagctggt cgatacattg tctgatagcg atcgaatcta tatcgttgct
    13681 tgcggaacta gttaccatgc ggggttagca ggtaaacaga ctttggaaaa attgacacag
    13741 attcctgttg aggttcattt agccagtgaa tttggctata acacacctct tctttcaaaa
    13801 aaaccattct ttattttctt gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg
    13861 gtaaaaatca accgtctcgg ctacccttca cttacgatta caaatgttgc tggttctact
    13921 ctttcgcggg aagcatcctt tacactgcta ttgcatgctg gacccgaaat cgcagtagca
    13981 tcaacgaaag cttatactgc acaaatagct gtactaactt tattagccaa agctatcggt
    14041 gataaaaaag aatttgaaac ttctcttgta ttcgatatca ctcatgaatt gagtttagtt
    14101 gctaatgcga tggaatcggt catcgcccaa aaagattatt tagaagagtt ggcagaagta
    14161 tatctcagcg atacaagaaa tgccttttac attggacgtg gtgctgacta caacgtttct
    14221 cttgaagctg ctttgaaatt gaaagaaatc agttatatcc aggcagaagg ttttgccgct
    14281 ggcgaattga aacatggaac gattgctttg atcgaagaag gaacaccagt gatcggaatc
    14341 atcagtgaag aagtgactgg tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt
    14401 ggcgctaaaa cactagttat tgtctcagag ggattagaaa aggaaagtga ccagctggtg
    14461 ctaccaactg tccaccctta tctaactact ctaaccacag tcgtaccgac tcaattactt
    14521 gcatattacg caacgctatt acggggattc gacgttgaca aaccacgtaa tttagcgaaa
    14581 tcagtgacag tagaataata aaagaacaag gaccagtgtc agacatctga cactggtcct
    14641 tgttctttta ttttttatga aaatatctct taatccagtc cgatttcttc tttgactact
    14701 gccgcgattt tatcgacata gtaattaact ttctcatcag taggcgcttc tgccatcact
    14761 cgaagtaatg gttctgtacc ggaaggacga actaggattc ttccttctga tcccatttca
    14821 gcttctgcct cttcaatcac ttttttgatt gccggaacat ccatcgcacc gtttttatca
    14881 cttacgcgga tattcactaa tttttgcgga tagattgtta cctctgctgc aagctctgat
    14941 aatttcttac ctgtctgttt catgatattc agtaactgaa ttcctgaaag catcccatct
    15001 ccagttgtgt taaaatctaa gaaaatcatg tgcccagact gctcgccgcc aaagttataa
    15061 tcgtttttgc gcatttcttc tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc
    15121 aaaccgattc cttccaccgc tttgtggaat cctaagttac tcatgacagt cgtaacgatc
    15181 gtatcttttt tcaatcgatt tttttctgcc aagtatttag cacagataaa catgatttta
    15241 tcaccgtcga caatgtttcc aagttcatcc accgcaataa tacgatcgcc atcgccatcg
    15301 aaagctagtc cagcatctgc tcctttttca acgaccatct cagctaaacg ttctggatgg
    15361 gtagagccta cgccgtcatt gatatttaac ccgtctggat ttgtgcccat tgtataaaaa
    15421 tctgtttcaa gatcggcaaa caaacgatta acacttgttg aagttgctcc gtttgctgca
    15481 tcgacacaaa tggtcaaacc ggaaaggtca ccgttaatcg tttgtacaag gaattgagaa
    15541 tatttcaata agccttctgg aaattcttct acagttccca atccttcagc tgatggacga
    15601 ggcaattcat ccacttcagc atctaataat gcttcaattt ctagctcttg gtcatctact
    15661 agtttaaaac catctgcacc gaaaaatttg attccattat cttgtgctgg attatgagaa
    15721 gcagaaatca tgacccctgc gcttgctttt tgcaaacgtg tcagataagc aacacctggt
    15781 gtagagatca cacctagctg gaacacttcg atacccaccg ataaaagtcc tgaaatcagc
    15841 gcttcttcta acatttgacc tgagattctt gtatcccgcc ctactaacac gcgtgggcgt
    15901 tttccttctt cttcatgctg gcttaggaca taacctccgc accgtcccag cttaaatgca
    15961 agttctggcg taagttcctt gttggcttca ccgcgtacgc cgtctgttcc aaaatattta
    16021 cccatagttg taaaattact cctttaactt aattttctga ggaatttgtt ctgctcattt
    16081 gtgttgattc gcttgtttct gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag
    16141 attccgaact ggaagaaatg gttgttgtcg atggttccgt tcctgaatca gcagatgaaa
    16201 tacttctact cgtacttgta ttttcttgtg tccgactgct cgtcgaagca gagctactgc
    16261 ttgacgtttg caacacgggt gtaatctcca cactaactga ttcgggcttg atacttaacc
    16321 cctcttctac tggtatttca attttacgag ttgttcgatg attgatacct gagacattga
    16381 tcggtacagc aatactgtca ccgatcgatg caagcaaatc gctagagccg cttacttctg
    16441 cttccagttt tgaaaaactg aaatcatagc tttctacccc ggcagctggt gttccttgct
    16501 gtatcccata aagtcgtacc gacttgcttg gcttgatcga atcagctctc actgttactt
    16561 gcactggatc agagacgata cttaatactt cccctgctgc attcagcgcc tgtattggga
    16621 cctcactatt aatcccatca tctgtgacca tcgaagcgtc cacatcagct actactcgtt
    16681 tgatttctgc caatgttttg tcacctgtag tgatagagac ttttttagga ctaacggaag
    16741 tatcatcaag ttcatatcca gatggtgtga acgttgaagg aatcaacgtt tccacatcga
    16801 aatcttttat tacctttttc tcaaccgtta ctgtaatagt cgatggttcg atctttgctg
    16861 tgacagcact gctcaaattt tgaattttta gcggaacttc atgtgttcct tcgcccagct
    16921 tagtgatatc tgctgtgacg cggaacatcc gtgtatcttc gtttgcctct gcattcaact
    16981 gaactcggtt tgcactggaa agtttgacag tcactgcatt ttcatatcca tggatatagt
    17041 agcgctttga atcataagaa atcttgatcg gcacccccgt aactgtttga ttatacgctt
    17101 catttccaga aagcgtattt ggaacggact ggccatttgc attgaagtac agcgcaatac
    17161 tgaacagcaa tgcaataaaa ctgtaaagaa tgcttgtccg tttctctttt gagatcattt
    17221 gcgatcccct cccttagtca taccgtctag gaaagattga atcacgtttt tcttgttttt
    17281 tgttttctct tcagcaatta gttctctacg caagattgcc aaatattctt gctgggataa
    17341 gccagccatc agttcattat ctaacgtgat gctgacgtcc ccggtttctt cagagacaac
    17401 gatggtaatc gcgtcactga cttcgcttat accgacagct gcccggtgtc gagtcccgaa
    17461 ttctttaggg atcaacatac tttctgatag aggaagatag gcgcttgcta ctgcgatctt
    17521 tccttctttt acaataactg caccgtcatg cagcggtgta ttaggaataa agatattgat
    17581 cagcagctcc ccagtgatat ctgcatctaa agcaatccct gtctcaatat actcatctaa
    17641 tccagtatga cgttcgatag tgatcaacgc accgattttt cgcttggaca tatattggat
    17701 ggctttgtca aaagacaaga tcatcttctc atcttcctgc tgttcgcttt ttgattgttt
    17761 gaaaagcgaa ctgcgtccaa gatgttccag tcctctccgg atttccggct gaaaaatcac
    17821 gatagcagct atcaccccgt acgtaatgac ttggttcatc agccaagaca aggtgtgcag
    17881 accaatgatt tccgctacga ttcggataac aataaaaata gctacacctt tcagaagctg
    17941 aatcgctttt gtgcctcgca gcagttggat cagtttgtat acaagatacc agacaaccag
    18001 aatatcaagt atgttcacca aaaaattcag agagaacaag tcaccggaaa acatttgctg
    18061 ccaatagtcc aaattaaaaa gttccccgat ttgaaatgac ataaaaagcc tctcttctct
    18121 tttgagattt cctctctagt atatcatatg ttttaatttt agaggctgat atgctcgtat
    18181 ttttcttgag aaaaacgcat aattttcaag aaaattgcaa gtatgcgttt tatttttctc
    18241 tgcaataaat atttttaaac ttcagagaat tattcattat attttttctg ttatttttcc
    18301 tttttttctt ttgtagcttg ttccattttt ttacgtcgtt ctgctctggc agctcttcta
    18361 gcagctcttc tttcttctgc ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt
    18421 acatcgatag attcctctgg ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt
    18481 gcagcatagt tgtaaaaacg tgttttagcg tcgtggacag tcttttcaaa taaagctttt
    18541 gcaagatttg gatttgcgtt atctagcgag gaaaagcgaa cttgtttcct catgaaatca
    18601 ggcatagcag agaaatcagg ttttttgtag tccaaagtca ttgggttctt gtttttttcg
    18661 accattgtag ggttatatcg atataatgac caataaccag aagaaacagc ttcttttgct
    18721 tctttaagcg attctttcat tccgccaagc aagccatgtg tgatacaagg cgtataggca
    18781 ataatcagag aaggaccagg atacttttct gcttcttcaa atgccttgat cgtttgcatt
    18841 tgattggcac ctgaagcaat ttgcgccaca taaacatttc catatgtcat cgccatcatt
    18901 cctagatctt tctttgaagc atactttcca ctagccgaaa atttagcgat tgccgaagct
    18961 ggtgttgctt ttgaggtttg accgccagta ttggaataga cttcgttatc caaaacaagg
    19021 atattcacgt ctgctccgct ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa
    19081 gcccatccgt ctccaccaat catccattgg ctgtttttta cgaatagatc ctttctcttg
    19141 tatatatcct ccagttcagg gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt
    19201 gctgctcttt gctgcgttcc ttcactctca aacatgtgta ctaaccagtc ttccatcaat
    19261 tctcgtagtt cttctgatac ttttggcaat acttctttga ttgaacttgc caaagcctcg
    19321 cgacgagtct gtgccgctag aagcatacca taaccaaatt cagcattatc ttccaataaa
    19381 gaatttgacc atgcaggccc ttgaccacat tcattggtcg tataaggaga agcaggagaa
    19441 gcaccgcccc aaatagaaga acagcctgtc gcattcgcga tcatcattcg atcaccgaac
    19501 atttgtgtca ataattttac ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat
    19561 tcaagaagcg gttgattgaa ttgtgtagac aagacagtat tctttccttt gactggattt
    19621 gctttttgtc ttaatgtcat tgcaaaagcc caattcacag cttgttcttt ctgttcttca
    19681 taaggcttca tcactaaagc tttttctttt gcgggacaag cctctacaca cagaccacaa
    19741 cctgtacagt cttccactga aacttggata cggtatttta cgccgtctgc gcctcgcatt
    19801 tctctcacga taaatccttc tggcgcttct tccatttcct cttcatctgc taaaaatgga
    19861 cggatggcag catgaggaca aacaaaggca cattcattac acatcgtaca gcgatcacta
    19921 atccactcag gtacttctaa agcgacgccc cgcttttcca tagaagccgt tcctaaaggc
    19981 atttcgccag ttgtcattcc atttgctaca agtgtaccaa cagagagttg atttccttcc
    20041 tgtgcattca ctggttcaag tatttcttgt atataaccag tgttcctttt attgattttt
    20101 tcaggcatct ccaacgttct ccaatggtct gggatcgcga cttgatgaag cagttcggtc
    20161 gtccgatcga ttgcttgtat gtttttttct acgatagcca ttgatttatg tccatagctt
    20221 ttcagggctt cttcttttag cagcggaagc acctcatcaa aaggaatgat gtcagctaat
    20281 ttaaaaaaag ccgtttccat tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc
    20341 aatctcatgg cattgatcgt atagaatcta atgttatttt cagccaagta acgttttaat
    20401 tttaagggca gatgcgtttc taattgttcg tcgctccaaa gagtattcaa taaaaaaatg
    20461 ccacctggct tcaatccttt gaccaagtca tagctatgca ggtaagctgg tgtgtgacag
    20521 gcaacaaggt ccgcatgctc gacaagatac gctgaacgta tcggcgtatc tccaaaacga
    20581 agatgagaga cagtcagacc gccagatttt ttcgaatcat aataaaaata gccttgcgca
    20641 tatttatcgg tgtgatctcc aataatcttg atagctgatt tatttgctcc aactgttccg
    20701 tcagatccaa atccccaaaa cttggcctgg aatgtttgcg gttctgtcaa atccagactt
    20761 tcctttggtt ccaaagattg ataagtcaca tcatccacga tccctatggt aaaccgtttt
    20821 ttgcgtatgc ttgggtcttt tttcaattca tcaaaaactg ctgagatttg gtcgggagtc
    20881 acatctttag accccaatcc gtagcgacca ccaataacag ccgggcgaag ttcagaatca
    20941 tataacgcac tttgaacgtc taataagaga ggttctccac ctgcccctgg ttctttactt
    21001 cgatcaagaa cagcaattgt ttttactgta tgaggcaact tttgcacgaa tgtttccact
    21061 ggaaaaggac gataaagatg gatattcaaa aaacctgttt tccgtccgtt tgcattcaag
    21121 taatcaatcg tttgttcaat cgtctgtgca actgatccca tagaaacaat gacttcttcc
    21181 gcatcgtctg ctccgtaata agtcaccaag tcataagaag ttcctcgtaa tcggttgatc
    21241 tctttcatat atttttgaac gatttcaggt aatttatcat aataggggtt gatcgtttcc
    21301 cgttgttgaa aatggatgtc tggattctgg ttcgtaccac taacagtcgg atgattggga
    21361 ttcatccctc gtttacgaaa tgaagctaat ttctcctgat tgatcagagg agctaattct
    21421 tcatattcca aaacttcgat tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa
    21481 ttcaaaaaag ggacttctcc ttcaatagaa gcaagatggg caacaggtgc taaatccatc
    21541 acttcttgta cactgctttc agctaacata gcaaatcctg tttgtcttgc agccatcacg
    21601 tctccgtgat ctccgaaaat attcaacgca tttgtcgtca ctgcccgact agcaacgtgg
    21661 aaaacacttg gcagtagttc cccagcaatc ttgtacatat tcggtatcat caaaagcagc
    21721 ccttgtgaag cggtatatgt agttgtcagt gcaccagttt tcaatgatcc atggaccaca
    21781 ccagctgctc ctgcttcgga ttgcatttct actactttta ctggttgacc gaatacgttt
    21841 tcttttcctg ctgctgacca ttgatctact aattcagcca tggtcgaact gggtgttatt
    21901 ggatatattg cagcaatctc ggtaaatgca taagaaatat atgcggctgc cgtattgcca
    21961 tccattgttt tcatttttcg catgattcat catccttgtt catattctct cttattatct
    22021 gttccattat aacgtaaaac atgccatttt tttcttttat ttataaaaat attttacatg
    22081 tatctatttg aattggtcat ctaattttta gacaaacagt ctaaatcgtt gccttttttt
    22141 gtcagacatg tataaactat aagcgaaaga ggtgttttaa catggatata tcagtgattg
    22201 atgcaacgaa aacaaataca caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa
    22261 aaatggtcga attcatcaac ttagcttgtc cgtattgccg acaatggttt gaagaatctt
    22321 atgagctatt agaagaagca gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg
    22381 ataaagaaaa agaaagctta cagcgaggga acgtgatgca ccggtatctc acaatcagcg
    22441 atggccaaaa agcgatcaaa gagatcaaac aaatattcga tactcaagac gagtggaaac
    22501 atctttctct ccaagaagta gcggattttg cagtagataa actaaagctg acagaacaaa
    22561 aagacgaaca gctgagccaa gctgtcatca acgaagctga gcaagcacat attcgatttg
    22621 ttccaactgt catcttgggt aaagaaatct ttgatgaatc tatttcaata gaggaattaa
    22681 aagaattgat ccaaacaaaa taaaaaaata aggagcgtg
//
LOCUS       DAZKOW010000042        22251 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000042 DAZKOW010000000
VERSION     DAZKOW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.042"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            321..2156
                     /gene="tdc"
                     /locus_tag="ACG912_001516"
     CDS             321..2156
                     /gene="tdc"
                     /locus_tag="ACG912_001516"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFQ0512844.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2461..3939
                     /locus_tag="ACG912_001517"
     CDS             2461..3939
                     /locus_tag="ACG912_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0512845.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3905..5236
                     /locus_tag="ACG912_001518"
     CDS             3905..5236
                     /locus_tag="ACG912_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0512846.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5350..7902
                     /locus_tag="ACG912_001519"
     CDS             5350..7902
                     /locus_tag="ACG912_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFQ0512847.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7909..9348)
                     /locus_tag="ACG912_001520"
     CDS             complement(7909..9348)
                     /locus_tag="ACG912_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512848.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9323..9505
                     /locus_tag="ACG912_001521"
     CDS             9323..9505
                     /locus_tag="ACG912_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512849.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9694..10056
                     /locus_tag="ACG912_001522"
     CDS             9694..10056
                     /locus_tag="ACG912_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFQ0512850.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10243..11100)
                     /locus_tag="ACG912_001523"
     CDS             complement(10243..11100)
                     /locus_tag="ACG912_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0512851.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11220..12131
                     /locus_tag="ACG912_001524"
     CDS             11220..12131
                     /locus_tag="ACG912_001524"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFQ0512852.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12138..13784
                     /gene="mdcA"
                     /locus_tag="ACG912_001525"
     CDS             12138..13784
                     /gene="mdcA"
                     /locus_tag="ACG912_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HFQ0512853.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13886..14185
                     /locus_tag="ACG912_001526"
     CDS             13886..14185
                     /locus_tag="ACG912_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFQ0512854.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14175..15851
                     /gene="mdcD"
                     /locus_tag="ACG912_001527"
     CDS             14175..15851
                     /gene="mdcD"
                     /locus_tag="ACG912_001527"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFQ0512855.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15855..16448
                     /locus_tag="ACG912_001528"
     CDS             15855..16448
                     /locus_tag="ACG912_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFQ0512856.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16472..17548
                     /locus_tag="ACG912_001529"
     CDS             16472..17548
                     /locus_tag="ACG912_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFQ0512857.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17573..18442
                     /gene="mdcB"
                     /locus_tag="ACG912_001530"
     CDS             17573..18442
                     /gene="mdcB"
                     /locus_tag="ACG912_001530"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFQ0512858.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18553..19317
                     /locus_tag="ACG912_001531"
     CDS             18553..19317
                     /locus_tag="ACG912_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFQ0512859.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19806..21899
                     /locus_tag="ACG912_001532"
     CDS             19806..21899
                     /locus_tag="ACG912_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0512860.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21924..22226
                     /locus_tag="ACG912_001533"
     CDS             21924..22226
                     /locus_tag="ACG912_001533"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0512861.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7216 a         3792 c         4733 g         6510 t
ORIGIN      
        1 ctattaaaaa ataaattgcg aatttttaac aatataaaaa aataaaaata tgtttagtga
       61 atgaaggaca gtcattttat ttgtttgttc gcttttttta ggttatctat tttagaagac
      121 tgatttattg aaaaattgtt cgcttttttg cgttgaatta tcagaaaaat ccgactttat
      181 ttatttttgt ttgtaaaaat ctacgtggat ttgatgttat aaaaaaaaaa ttctcatttt
      241 tggcgctata gtgaagaggt gttacagata aacacatgta tctgcaagac attgaccaac
      301 aaaatatgga gggaagaaaa atgaaagata tggatatcaa ggccgtcttt ataggcgaca
      361 aagctgaaaa tggaccagtt tataaaatgt tgttgaacaa gatggtagat gaacatcttg
      421 gatggagaga aaattatatt ccttcagata tgcctgcaat tagcgaagga gataagctca
      481 ctccagatta tctagccacc cgcgaccata tgatagaggt cttggatgaa gtcagtcagc
      541 gattacgcgc aggatcgatc ccatggcatt cagcaggccg ttactgggga caaatgaatg
      601 ctgaaacatt gatgcctgct ttattagctt acaactatgc gatgctgtgg aacccaaata
      661 atgttgcatt ggaatcttct atggcaactt cgcaaatgga agcagaagta ggacaggatt
      721 ttgcttcatt attcaatatg acagacggat ggggacacat tgcagcagat ggttctattg
      781 ctaatcttga aggattatgg tacgcccgtt gcatcaaatc gattccttta gctgtcaagg
      841 aagtgctgcc tgaaaaagtg aaaaaaatgt ctgagtggga acttttgaat ttatctgttg
      901 aagaaatttt ggaaatgacc gaaagcttta cggacgaaga aatggatgag gtcaaagcag
      961 cttcttcccg tagcggaaaa aatatccaaa gattagggaa atggctggtc cctcaaacaa
     1021 aacactattc ttggatgaag gcgttagata tttgcggagt cggcttagat cagatggttg
     1081 ctatcccagt tcaagaggat tatcgaatgg atattaatgc tttggaaaaa acaattcgtg
     1141 aattagctgg acaaaaaatc ccaattctag gtgtggttgc tgtcgtagga acgacagaag
     1201 aaggtcaagt agacagtgta gataaaatcg tccaattaag agaaaggtta aaagatgaag
     1261 ggatctattt ctatttacat gttgatgccg catatggcgg ctatgcgcgt tcactgtttc
     1321 taaacgaagc aggagaattt gtgccgtatg cttccttagc tgaattcttt gaagaacatc
     1381 atgttttcca ccactgcgta acgattgaca aagaggtata tgaagggttc agagctattt
     1441 cagaagcgga ttctgtcacg atagatcctc ataagatggg ctatgtccct tatgctgctg
     1501 gaggaatcgt aatcaaacat aaaaatatga gaaatatcat ttcttatttc gcgccatatg
     1561 tgtttgaaaa atcagtaaag gctccagata tgttaggagc ttatattttg gaaggatcaa
     1621 aagctggagc aaccgccgcc gcagtctgga cagcacatcg tgtattgcca ttgaatgtga
     1681 ccggatatgg tcaattgatt ggggcttcaa tcgaggcagc tcaaagattc agagaatttc
     1741 tcgatcatct gacttttact gttaaaggaa aaacaatcga agtctatcca ttgaatcatc
     1801 ctgattttaa tatggtcaat tgggtattca aagaacaagg ctgtacagat ttgaacgcta
     1861 tcaatgaatt aaatgaaaaa atgttcgatc ggtcttctta tatggatggc gatgtttatg
     1921 gtgaacggtt tattacttct catactacat ttacacaaga agattacggt gattctccga
     1981 ttcgttttgt tgaaagaatg ggtctaacga aagaagaatg gaaaaaagaa cagaaaatca
     2041 ctctgctacg tgcggctatc atgactcctt atttgaatga tgatcgaatt ttcaattttt
     2101 atacaaaaaa aatcgccaaa gcaatggaaa agaaactaaa cgaaatcatc caatagaact
     2161 gatttttgtg tgaaagaata acaagagaca tatggagaac agacaattat ttgcttcatc
     2221 aaaacaattg tatttccccc tgtttttcat atattcctcg ccaagatatt cttttttata
     2281 aaacgttcta taagttggat cgttcgtttc atgtatcaaa cccagttcac taagatcgtt
     2341 caatggatgt ttcggtgata ctgggttttg tactgaatcg atgaaacctt gtcatttctg
     2401 ttcttgaaca aaaataaaag aaaacaaaaa catatgtcgc tattgaaaga aggaataaag
     2461 atgaaagata ctggttcatc aaagcaaatc acgaccatga cctttatcgg aatgacctgc
     2521 gcccttgtag caagtgtacg aaatattcca gatgtagcag caacgagctg gacgatgatt
     2581 ttttacatgc tggttgcagt attattgtac gcttttccaa tctcattgat ctctggtgaa
     2641 tttgctggaa tgtttcccca aaaaggtggt ccagaacttt gggtatcgaa tgctctagga
     2701 aaaaaatggg ggttcgttgt ctcatggctg ttatgggtcc aaatgtttcc tgggatggtg
     2761 atggtcgctt cagcactcgc tccgttgttt ggtaatatta tcgataatgt gccgcttggt
     2821 ttgaacagca aatttacatt agttgtcatt ttagtcgttt attggatcat cacttttttg
     2881 aatttgaaat ttgatatggc aaaaatcggc ggaaaagtcg gtgtttggtt aggattgtac
     2941 gtgcctttga cgatgatgct cttattaggg tttgctgcgt ggatcaaagt tgggatcgta
     3001 ccaaccggaa cattaggcag tttttcatgg gacaaattaa taccagatac tacgactgca
     3061 tcctcatttg tttattttgc gccgatcatg tttattttta ctggtatcga aatgtcctct
     3121 gtgtatatca ctcgattgga aaatccagtt aaaacgtata ttcgcggagt atttgccgcg
     3181 ttaattttta tatttttcgt caatattttg aatgctttag ttgtagcaaa tgtcgtacca
     3241 aaaggacaaa tggaattgaa taatatcgcg caatctattt cgatttattg ccaaattctt
     3301 ggtttgccac atcttattgt gaatctgttt agtttacttg tcttcattgg tgtagcagtc
     3361 caattatctg cttgggcctc aggaccagca aagacagtga cagaaagtgc tagaaaaggt
     3421 gcgtatccac caaaattcaa tttttggaag acgaatcaat ttgacgtatc caaatccgtt
     3481 attttgactc agtctgtcat tatctcaatc tttgcacttt tttatttgct gattcctggc
     3541 gtcaatcaag catttttgat gctcgtaaat tcaactactg ttatctactg tatcgtctat
     3601 gtcatcatgg ggatcgcagt tttgagattg cgttatacac atgcaaagtt gaatcgacca
     3661 tttagaattg gaaaaaaaga gaaaaagagc aatcttggag tttggactgt cgttattgtt
     3721 ttatttgcgg caatcggatt ttctgtcgga ctgacgatga aagctggaac atggatcaat
     3781 ctaattgcag tcacagccat cagtgtcatt ttgtttgtag taccactctg tattgaaaaa
     3841 ataaagaaac cttcttggga acaggaagtc gccatgctgc ttgaaaaaga aaaaggtgaa
     3901 cttgatggaa aaaccggcaa tgaaacggac tctaactaat cgaaatatcc agttaatcgc
     3961 attaggtggc gcaattggaa caggattatt tttaggttca gcaaaggcaa ttgcatcagc
     4021 agggccagct gttattctcg tttatttgat atgcggtttg gctatttatg gtttgatgcg
     4081 ggccatgggt gatttatttt tagcaaatag tacatttaac tctatcagtg atttcgttga
     4141 ctattattta ggcaaaaagt gggcattttt tgtcaattgg acttattggc tttgctggct
     4201 tggagctgga cttgcggaat taacagcaat aggattgtat atccatttct ggtttgtaca
     4261 tgtaccagct ttgataagcg gaggtagcgc actaatgatc ttaatggcag tcaaccttgt
     4321 tgcagtcaag tggtttggtg aattggaatc ttttttctca atcatcaaga tcttggggat
     4381 tttccttttt atcttattgg gtataggatt gagtgtctca gattttcata cccagtctat
     4441 cggaatcaaa gaaaattttg gacagatcga tgaattacta ccgtttggct ttgctggaat
     4501 ggtagtctca tttcctatgg ttttattcag ttttgctgga attgaaatga tcggcttaac
     4561 cgttggcgag acaagtgatc cgcaaaagaa tttgaaaaaa gcaatcaact gtctgccttg
     4621 gcgtattctc tttttctata taggaacgat tttgtcgatt ttatttgttg ttccatggca
     4681 ttgtttagat ttaaaagaaa gtcctttagt gactatgctg caagtgatcc attgccaagg
     4741 cggagcagca attgtcaatc taatgatttt ggttgcttct ttgtcctcag caaatagtgc
     4801 agtctacagt actagcagga ttttatatca aacagcaaaa gaaaaaagtt tgccccattg
     4861 gttttgtgcg ctttctaaga aaggcgtacc tttacacggt attttaatta caggaatgtt
     4921 attttttgcc ggtctttgtc tacatttttt tgtagctgat agtcgattgt tttttcagct
     4981 tttggcaggc atgacaacga gttgttttct atttgtgtgg tcttcgatct tgtgtgcaca
     5041 tgttcaattt attaaagaaa agaaacggaa cactacacaa agatataaag atcttgcttt
     5101 gttggtgttt tttacttcaa ttgctttcag cttgttattc gaaagcacga cacgctttat
     5161 tcctttttta ttggttatct ggtttgtatt cttaaatttt gtatataaaa gaataagcaa
     5221 tagaaatatc agttaggccc agatacattt tgcaaaacga attatttcag acccccctga
     5281 aataatcgat acgcctgaaa aaacttccct gcgtataata acagtatcaa ttaaacagga
     5341 ggggatagga tgaactggta cagattgacg gtagaagatg tattgaaaaa ctatcaaaca
     5401 tcaacagaag gcttgtcgca agaagaacga cagaaacgcc tgactgatca aggatataat
     5461 gaaataaaag caaggcagca gacaaaacga tggaaaaaaa tagcaaagca ttttactgat
     5521 ctgctgatga tcgtgttgat cattgcctct tttttgaaat ttgcttctag tgaatatatt
     5581 gaaggaagta ttattttatt cgtagtgatc gtcaatggac tcgttagtta ctggcaagaa
     5641 agaaaagcgg aagaatcatt gaatggtttg aagcagctga tgggacaaga agcgatagta
     5701 ctatcaaacg ggatgcaaga aaaaatccct gcgaaagaac tggtaaaagg agatgtcatt
     5761 gtcttacgtg ccggagatgt cgttccagca gatatccgat taatcgaagc acatgattta
     5821 ttagtggaag aatcgatttt gacgggagaa tcagaagcat ctgaaaaaag tcatgccgtt
     5881 ttgacagaag aagaatcgat cggcgaccag aaaaatatgg gtttttcagg aacgcttgta
     5941 caatctggtt ctgcagtcgg tgtcgttgta gaaacaggaa acacgacaga aatcggtaaa
     6001 atcaatcaag cattgcaatc ggtcgaacaa caaacgacgc ctctgattaa aaaaatcaac
     6061 cgattaaaca aacaaatttt tcaaggcatt ctttgtttga gcctattctt agttattttt
     6121 acgacatttc gatatggtat ggattggcat atccttcttt cggctacgat tgctttggtg
     6181 gtgtcaatgg tacccgaagg attgccagct gttttgacca tgattctatc cgttggagta
     6241 catgaaatgg caaaagaaaa ggcgatcatc aaaggacttc cttcggtaga aacattaggt
     6301 tctatgacag tgatctgttc agataagaca ggaacattga caaaaaatga aatgacagtt
     6361 gtggatgtag ctgcaaaaga agaagcctgc gtgttgtcca ttatgaaaaa ctgtcaggaa
     6421 ttaaaaacaa aagaagaaca gaaaacagaa aatttaagtg gcaatccgac agaagttgcg
     6481 ctgattcgac acacagaaac agccaatcta cctttgaaaa aaatagaagc aaagattccg
     6541 ttcagttctg aatacaaata tatggcaacg atgcatgcgg aggaaaaagg agcggtgatt
     6601 tacgtcaaag gtgcaccaga agtattattt gctaaatcaa ctctttcttc tgcagaacaa
     6661 gaagcatgga gtcagacagc tgcagaattt gcgagaaaag gtcagcgtgt tttaggattt
     6721 gcttataaaa aagtagattc gaaacaggaa ctcacgcatg agactttaac ccaattaact
     6781 tttgcaggga tcgccggatt gatcgatccg ccaaaagaaa gtgcagtcaa agctgtaaag
     6841 gaatgccaac aagcagggat ctcagtgaaa atgattacag gggatcacaa agacacagca
     6901 aaagcgattg cggaacaaat tggcttaaaa catactgcca aagttttaga agggatcgat
     6961 ttggatctta tgtctgatga agagttgatt cagcaagtgc caatagtcga tgtctttgct
     7021 cgaacgacac cagaacataa actgcgaatt gttaaagctt tgcagaaaaa tggagaaatc
     7081 gtcggaatga ccggagacgg cgtaaatgat gcacctgctt tgaaaagatc cgatgtagga
     7141 attgctatgg gaatcaaagg tagtgaagta agcaaacagg cagctgatat ggttttagga
     7201 gatgataatt tccatacgat tgcaaaagcc gtaaaagagg gccggcggat tttggataat
     7261 ttgcaaaaaa ccatcaattt cttcttgcca actgcattag ctcaaggatt gatcttgatt
     7321 tgggcactta tgctgaatcg cccgctacct ttgtccccag tacagatttt atgggtcaat
     7381 atggtaacga caatcacttt atcttatgca ctagggttcg agtcgccaga tccagaaatc
     7441 atgaagcgcc caccaagaga tccaaagcaa ggaatcctat ctggttatca tttgtttcgg
     7501 atcatctatg tctccctact gatcatggtt cctgcctatt tattggctat gcagtttgaa
     7561 gggcaaagct tacagcaaac cattcttctg caaaatatcg tcttagctca agctgtttac
     7621 atgatcaatt gcagagagtt gtccaagccg tcattgaacc aaggtctttt gcaaaacaaa
     7681 gcgctttttc tttcattagg tatccttgct ttattgcaag ggatggttat ttttctacca
     7741 gtcggccagc agctgatcgg tacagtaagt ttaacgatga cacaacagtt attgattgga
     7801 gcaaatgtga ttcttctatt cttagttgta gaaatagaaa aagggatcat gaacaaactg
     7861 tttcgcaaaa aagaaaaaac agctagagaa tacagtcgct agccgttact ataaaaaagt
     7921 tttgaaagtg agttcagtca aacgattgag ctcatttttc gttggaatag aagaaatgta
     7981 tacaataggg atatctagag aatctagagg gaaattggaa atcaagatat ctgttttttc
     8041 aaaagaaata gtggaaagat cggttagttc gataggtaag aagcagacac gttttcctaa
     8101 gtaatctttt aattcctgct gcaagaacgc cttccatgaa ggttctcctt ggaaaataaa
     8161 atagacattg aacaaattcg gtttgataga aaaaatcgtt tgttggatca gtagagacaa
     8221 gagttggaga aagtaatttg gttcattgat ttctatttct ttttttagat cgttcagtaa
     8281 attctctttc tgttgataca agtgtgcata ttgctgatca tatgtttcta aaaaacgatg
     8341 gtattgacgg tccaatgctt caatcaatat cggcgattca tgatatttta atatgaggag
     8401 agccaagtta tcagtcagag atgttttttt tagttgatca agagacagct ctgaagctag
     8461 ttcctctaca aacgtttcga ggacctctaa aaatggagaa tgatgcaggc agcgagcggt
     8521 ttcttgtcca tagttattgt tgtaattcca actttccaga aagcagaaag aagcaaaaga
     8581 aagctcagtg tctttaagat aaataccttc cgttaaatac aattttttca atgcagagtc
     8641 atacagggag gagagtgtgc tgttggtatg gatagaagaa tctaatcggc aattcgcttt
     8701 tatacggatc gtattgataa agaaccaaca tgttccgaaa atctcttctg tttcaaccgc
     8761 tagttcagtt tgactgaggt ttcttaaaaa ttgaaaataa tgggattcat ggatcgaata
     8821 atcctcgaaa aaatagttgt tatgagtgaa aggaatcagc aatcgatgat aaaaagtacg
     8881 aatcgcacgc tcttttccaa gaatacaagc tgtatatttc gtcaaatcga tctgcaagtc
     8941 gtaacttgct aaacgctgat tgattttagt cacatgtctt ttcaatgtcc cataagaaag
     9001 cccatggtcg gctagaaaat cagggacagc aacttttttt gtaaaaagca gatttttcag
     9061 cagttcgaca ctgactgatt gtttgaaaaa aatctcccaa aggtcatttg ttaataaatt
     9121 ttgctggttt tgcaaactga ttcctgcttg atcgctgatg atgtcccagc cttcgggcag
     9181 ctgagagcga atagattgaa ggtcagaaaa aatcgttcgt tcggttgtcc ctatgatttc
     9241 agctaattca tttacggtta tctttgtatg attcagcaat tgttctacta aagaaacatg
     9301 acgtcgaata tctttttcgg taatgatttt tttcattagt gaatacatca atcagactcc
     9361 cttctattct aagaatacgg agaaaataaa cggtggtcaa gtttcaactg atttttcaat
     9421 cattgtttat ttttctggag catttactga tgataagaat ttcatcatga acttgaaaaa
     9481 tgataccact aaaatcaaaa aataggtggg caaacaataa taaacatttt aatagtagat
     9541 ggtagcaaga ccaacacagg cggattgaac atttaagagt tgataaaaaa gaaaaatatc
     9601 gaagtatttg attagcaact acttgtattc atttttctat tcttacgtgg tatctttgaa
     9661 taaagcgaat caataagaaa gagatggaag cagatgctaa cgaaaaaaga actaatcgat
     9721 cacatcaatg aaacatacaa tttgtctcca gaatatattt ttaaaaaatt tcctgattat
     9781 tgcgtattca gacatcaaaa aagcaaaaaa tggtttgggg tcctgatgtc tgttccagaa
     9841 gaaaaaatct atgatgatgc aagtgaccat aggatcatca atattcttga tttgaaggtc
     9901 gatcctgaac ttggcggaat tttgcgagga aaagaggggt attttccagc ctatcacatg
     9961 aataaagaac attggatctc tattgatcta cgtaaaatag aaaagctaga tgaactagca
    10021 gatttgattg atggaagttt taaatcaacg caatgaatgt ctaggacaat agagaagagg
    10081 ttgtgacaga aatgtttagc ttcaagaaat aagaaggaat ttacgaaaat tgcttctcaa
    10141 atttttggga aatttcggct tattttctaa gaaattactt ctggaacacc gtttgttcga
    10201 aaaaagagct gcgacaagaa ttttgtccca gctctttttt ttttattttc ctaaataaaa
    10261 ttcccgcatc gtttttgcaa tcattttgat caaaggatga tcttctgtga agctagaaga
    10321 taaataaatt gttcgtgtca agggcaaatc tttgatttcc cgcaagacga aaggatcatt
    10381 tttatcgtaa gaccaagaaa cttttgggaa aaagctaaca gctaatcctt gcttgacaat
    10441 agaacgtaat gtcgcaggat catctgtcgt cgaactgatc gtcaattcga tacttttcgc
    10501 ttcaagtgct gcatccagtg tatgacggaa ggcgttgctc tttgacagca tcaagatggg
    10561 ggtgcgttgg atatcttcta attgcagatg ttctttttta gcaagaggat gtttcttagg
    10621 aatggcgaca taaacaggtt ctttcatgat aggaatattg gtcatgccag gcttcatctc
    10681 cgaagtgatg tacagtacag gatcggtaat atcatctggt aaatgatgtt gcatgatcgt
    10741 taattggata tcaggatgct tttccataaa aattcggacc aagttcggaa tagaaacaga
    10801 ggctacctct aaatacaata tgacggaagt aacgatcttc tcattgaccg aagacaataa
    10861 tttgattcct tcttctaatg tgtctaatgt cttgactgca tatggataaa attcttctgc
    10921 ataaacggtc gtctcaatgt ttctgccgct atggtaaaac aaagggacac ctaattcgtt
    10981 ttccaaatgt ttgatcgcac ggctcaaggc aggttgagaa ataaataatt ctttggctgc
    11041 ttgggtaaca ttttttgttt cgaatacttt tttaaaatac tgcagatgaa gcaatttcat
    11101 aattatccat cccatccata cctttttgtt atggtttttt ctttttttgg cattttttat
    11161 tatagcagaa attgactaag atagcagtgg aaaaagtaag tgctgaggag gcgaggctca
    11221 tgagtacagg tttcttattt cctggacaag gcagtcagaa gttagggatg ttagcggata
    11281 tccctgaaat gtacatggaa cgtttagaaa atgtgacggg gtatcggtta gttgataaag
    11341 aagaaaacta tcaagatacg gtttttatcc agctagcatt actgacaaaa gcagtatttt
    11401 atttagatga aatggaaaaa aacggtattc agcctgatat tgcagcagga cattcgattg
    11461 gtgctttttg tgcagctgtt gcttgtcgat ctttgagatt tgagaatgca gcggaactag
    11521 tctatcaccg cgcagtctta atgaaagaag cttatccaag cggttatgca atgggcgtag
    11581 tagtaggtgt gacgagaagc gaagctgagg aaattgtagc aaaaacattt gatcctgacc
    11641 atccagttta tctgtcaaat gaaaattgtc cgatgcaaca tacgttatct gggagtatcg
    11701 aaggtttatc cgttacgttg gcagaagcca aacggcacca agcgcaaaca gcgaagctgc
    11761 tgaaagtccc tgtgccatct cactgtctct tgatgaatga tacggtcaag aaattcaagc
    11821 cgtatatgca gtctgtccat ctatctgatg ctagatgttt gtatataaaa aatacggatg
    11881 gaagagccac tcaggacgca gaagagattc gtaaagattt gttggaaaac ctagctttcc
    11941 ctgtgcagtg gaaccaaatg atggacttgg caaaagaatt aggaatggat ctgacgattg
    12001 aatttccccc cggcaatact ttgacaaagc tgattcatac aaaatttggt gtagataaag
    12061 ggatccgtac gatcaatcta gaccaacatg gtattgatga cgccacgttt ctatatcaaa
    12121 aatggagatg actaattatg gaaaaaccaa accgcaactg ggcttctaaa cgcgaaaaaa
    12181 aagcgcagcg attggaaaaa atcagcaact tgctcaatgg caagtatgta gaaactgaaa
    12241 aaatcgtaga agtaatggaa aaactgattg ctcccggcga caaagttgta ttagaaggcg
    12301 acaatcaaaa acaagcaagt tttctttcag aaagtcttga aaaagtagat gcagaaaaag
    12361 tgaatgatct gcatttgatc atgtctagta tctcacgacc agaacatttg aatatttttg
    12421 aaaaaggaat cgcaagcaag atcgatttct cttatgctgg tgcacaaagc ttgcgtgtcg
    12481 cccaaatgat tgaagacggc acaatgaaat taggtgatat ccatacttat ttggaacttt
    12541 atggacgctt attcatcgat ttgattccaa atgttgtttt agtagctgca gataaagcag
    12601 ataaagacgg caacttgtac acaggtttca atacggaaga aacaccaaca atcatcgaag
    12661 ctgcagcttt caaagatggt atcgtgatcg ttcaagttaa cgaagttgtc gattctttac
    12721 cacgtgtaga tatccctggt gactgggtag acgttgtcgt gaaagcagat aaaccttatc
    12781 aattagaagc tttattcaca cgagatcctc aaaacatcac agaattgcaa attctaatgg
    12841 caatgatggc aatcaaagga atctacgcag aacacggcgt acaatctttg aatcacggta
    12901 ttggattcaa tacagcagct atcgaattgt tactgccaac ttatggtgaa tcactagggt
    12961 taaaagggaa aatcgcgaag aactgggtat tgaacccaca tccaacgatg attccagcca
    13021 tcgaatcagg ttgggttgaa agcatccata gttttggtgg cgaagttggt atggaaaaat
    13081 atattgctgc tcgcccagat gtcttttttg tcggtaaaga cggcacgatg cgcagtaacc
    13141 gaacattatc acaagctgca ggacaatatg ccatcgatat gtttatcggt tcaaccttgc
    13201 aattagatta cgaaggaaac tcttctaccg taacaaaagg acgtttatct ggttttggcg
    13261 gtgcgcctaa catgggacat aatcctggcg gtcgtcgtca ttctacacca gcatggcaat
    13321 cacttcgcga caatgatgat ccattaggga aaggccaaaa attagttgtt caaatggttg
    13381 aaacatatgg ttcaaataaa aaaccagtct ttgtcgaaga attagatgca ctagctgttc
    13441 aaaaacaagc aggcttagcc aatgcaccag tgatgattta tagcgaagat actacacata
    13501 tcgtgacaga agaaggaatt gcttatttgt acaaagcaaa aacaaaagaa gaacgacaag
    13561 cagcaattgc agcaattggc ggtgttacac ctcttggaat gactagcacg aaagaatcac
    13621 tagatagctt gcgtgcagcc ggcatcgtga aattaccaga agatctaggc atcaaacgta
    13681 gtgaagcgaa acgttcatta ttagctgcac aaaccatcga tgatttgatc gaatggtcag
    13741 atggcctgta tgaaccacca atgaaattca gaacttggtc ataaaattgg cgagaaaaac
    13801 cttttgtcgc catttaccca gatggaagaa gaaagacgaa taagcagaac gtgtatgctc
    13861 tgttttacaa aaaggggaga gatccatgga acaattagct tataaatatc caacgaaaca
    13921 gccgatcaat aagaaaatcc atgtaggagt ggtcggttca ggagatttgg aaatcttgat
    13981 gtttccaaca gaaaaaacag aatctactgt aaaaatttgt acaggaagcg atggcttcgg
    14041 cgaagtatgg aacaatgtat taacgagatt ttttgatcgt tatccaattt cagcagatat
    14101 cgtgatcaat gacttcggtg ctacaccagg agttgtgtac ctacgattga cgcaagcaat
    14161 ggaggaactt tcagatgaaa aatagttttg tagaattaaa cgccagagaa cgagtgaacg
    14221 ctctacttga caatggacgt gaattgattg atccattcga tcaaatcatc gcgccaagtt
    14281 taccagcaca aggaatcgtc cctgaaagtg atgacgggat cgttgtcagc cgtggcacga
    14341 tcaatggcaa acaagcagta gtaatcgcta tggaaggaaa attccaaggt ggcggtatcg
    14401 gtgaagtttc cggtgcaaaa atcgttgctg cacttgaaca tgtgttagaa gaaaataaaa
    14461 aaggaaatga gatctttcca gtcattgtac tagatacagg tggtgttcgt ttgcaagaag
    14521 caaactacgg actgttatct atttcagaaa tcggaaatgt aatcattgct ctaaaacaat
    14581 atgtaccagt catcggtttg attccaggac gtgtaggatc atttggcggt atgtcgatta
    14641 cttcagcatt aatgagttat ttaatcgcaa caaaaaaagc gagagtcggt ttgaatggac
    14701 cagaagttat tgaacaagaa gcaggcgttc gagaatttga ttctagtgac aaagacttga
    14761 tttggaatac attaggatct cgtcaacggg tagaatcaaa aatcattgat gaattagtga
    14821 ctgatagtgt agaagcaatc aaagctgctg tcatcgcagc aatggacgaa cgaaaagaca
    14881 cgcatcgcag tgaaagctca gcattttatt tatcattact tgattcttta gatctttcaa
    14941 aaccaatggc cattgacgat tacaatgaag caattgctaa ccatcaagca aaatcaattg
    15001 acttaccaga agtagcagaa ggaaatgaac cagctgcagc aagtgttggt tacgattggt
    15061 ttgcggcact aactggcatg aaaaatccaa cttcttctat tagtactgtg tattatggta
    15121 aatcaagtaa attcatagaa gaagccatcg tgacatcaat cgtacctgat cctaaaaacc
    15181 cgatgtaccg tgtacgaaac ggtgaagttg gtttagttga aggtttccgc atgggacaaa
    15241 ttttggatca cgtttacgaa gaagataaag acaaaaaagt gaaacggcca atcatcgttg
    15301 tgatcgatgt accaagccaa gcatacggct ataacgaaga attgatcggt atccatgtgg
    15361 ctttggctaa tagtgctgct gcttatgcaa aactccgtca agctggacat ccagttattg
    15421 gattgattgc aggaaatgct atttcaggtg catttttagc acatggatta caatcaagcc
    15481 gcttgattgc tttaaacaat aaagaaatta ccgtccaagc gatgtctaaa gaaagtgctg
    15541 cacgtgtgac aaaacgttcg gttgctgaaa tcgaagcagc tgctgaaaaa gttccagcta
    15601 tcgcttacga tatcaataat tatacaaaac taggagcgat ctatcatttc ctagaagata
    15661 tcaccgacca agctgcttca gaacaaaatg tcagcaaagt catcgaagca atcaatgaag
    15721 ccattcaaga tgtccgcaat acaaatgaca cgatgctgac aaatcgatac acaaacgaaa
    15781 cagccaaaac attcggacgt gtcgaaacaa ataaagtccg tgccaaaatg gacgaagaat
    15841 gggatgcata aaaaatgatt caagcacatg atattgtcct ttttgataaa catgaaatag
    15901 aagggttcac cttgcctgaa tgggtcgttc taactgaaaa gatgttcgga acagttcgtc
    15961 gtgcgtcgac ggatcacgca gaaatactac cgattggttt acgtggacct gcccgcaatc
    16021 agagatggtc aacagaaatc tcaaaacatg ccgtcaaaca agtcattcat ccgtgggaca
    16081 tcatcgaaca agaatcgtat agacaagaga aaatcatgga ttatcctgtt tatcagcaat
    16141 ttcatgctgc tagaaacata ttatcaggat gtaaatgggg aatcggcggc agcttaggat
    16201 ttgaattatc gactgggatt ccggcagtaa aagaaaccag tgattttgat ttgctgctgt
    16261 acgcaaattc accgattgaa ttgccaattc aggtaattca atcgcatcct gctttttttg
    16321 agcaattcga tacccaagta gtgactcggc aaggcggttt ttcactaaaa gagtatttga
    16381 gagagccaga gaaaaaaatt ctattgaaaa cagtcgaagg acctaaatta accaaagaac
    16441 tttggtaata aagaaaaaga gagagaaaga gatgtttgaa aacaaaaaaa tcaaaatcaa
    16501 cgtaagtgac ttccagaaag tatcagctgt aacggctgtt atgatgcaaa cgatattgag
    16561 ctttgctttg gcaaacagcc acagcgcaac aaatacagct tttcttggta ccttttacgt
    16621 catcgttaaa tacacagcac caatgtttat ttttgcaatt gtttataata tggtaaaaac
    16681 aagtcagcat ttgtcttatt gggaattttt gaaagaaaag ttttttgaac tagtcgttcc
    16741 gtatcttcta tggacgacgg cttatctttg gctatttcca gcagtccaac aacaaactcc
    16801 gtatacggat actgcaagct ttttactgaa atatgtcaca ggtgatggag cgcctcattt
    16861 atggtatacc gttatgatgc tgcagatcca attgttcatg ccgttttttg tttggctcgg
    16921 ttacaaagta tttgctaata aaaaatatgt atggccagta ttgattggtt caacgatttt
    16981 atatgtcgca tggtatctgt tctatcaaac acaagtattg actgggcctt atcatgacag
    17041 ctggtactta ttagatcggt ttgttgctag tttcatgatt tatggggtag ccgcatttgt
    17101 ttatcacgaa aaagtctatc aatatttgga cagagtacgt tatcttttct tacctgttgc
    17161 cttagttatc gcgttctttt ctgtccgttc attgctcgca catccaggag acttgagctt
    17221 tgcgaatgca ccttacttga atacgatcca aagtttatac agtttagtca tcatctttgc
    17281 tgtcttcata ggcgcttcca aaatgattgt aaacgattcg ccaaaattgc cattgttcaa
    17341 atggttgtct gtttatgctt atcgtacgta tttagccaat gtatttgttt tccaagtact
    17401 gttattattg tttaaagata gttggctgca gttaccttca ggagtcatga ttcttgtagc
    17461 ttatctaatg acggcaagtt gtgcatttgc tttaagctgg ttacttcata tcatttgggt
    17521 agctatcaaa aaaggtttca gcaagtaata gtaggaagaa ggagtgagtg tcatgataaa
    17581 tgacgaacga aaaaaagcag tgactcagca actcgctgcg ttggtagagg aagcattgat
    17641 cgcagaagta acattaagtc caaaacctgg attagtggat gcactggatg cgggtgcaca
    17701 ttccgatatg gactatgctt tatttcttaa gtctgctaag acgctaactc ctttttttga
    17761 agaaatggct caaatagcat ggcatcatgc tattgatcag gagttaagag aaagaattgc
    17821 tgagattggg cgaaaagcgg aaaaggcaat gctgattgct acaaatggca tcaataccca
    17881 taaaggagcg atttgggcaa tggggctatt gatcagtgtc attgcacaaa aaatcagtat
    17941 ggaacagcct ttttctttat cgaaaatact tgatgatgtc agcaagcttg ctgcttttcc
    18001 tgatacaaaa tatcgtcata aaaaaaccac tcatggtcat agggtgaagc aaaaatatgg
    18061 cgtaaatggt gcatatgaag aagcgatatt agggtatccg catatcaggc ttgcaattca
    18121 agcagctaag gagctagata atgattcata tgtacagaaa caaatgcata tgttgttagt
    18181 tttgatgagt tcgctagatg acacatgcgt attgcatcgc agtgatcaga aaacgttagt
    18241 ggaaatgcaa aaacttgctc atgctgctag tcaaagtgct ttaccaaatc gtccatttac
    18301 agcactgttg cacttttgtc aggaaaacca aatctctcct ggaggaagcg cagatctctt
    18361 gtctgcaagc ttgttcatgc tgaagataga ggaattatgg actgccgaca catacatttg
    18421 tccgatgtta ctcaatgact aaataagaca aaattgctaa aaaaacagtt tatgcatctg
    18481 gttattgact cttcctttta gcaaggctat cataaaaata gttttttgta ataaaagata
    18541 acgaggaaga caatgaaaaa aatcaattat ttagcaatca ttttgttagt gggcctatgt
    18601 ttgtttgctc tgccgaccca aggattcgct gtcgcgggtg gtcatggtgg aggtacatct
    18661 agcggtcgaa gttcgggcag aggttcattt ggtggtcgtt catatggggg cagttatttt
    18721 ggcagtcgtt cttacggcag agattcttat gggtattatg gtccaggtac aggtccttct
    18781 agttatgagc aaacgggatt tcttgcatta gctgttttgt cagtaggagg ggtttctgct
    18841 ttttctatcg tcaagaataa aagacaaaag aaaaggctga aagcggagat attgtcccgc
    18901 atgtcaggaa cggctaaaga aaagaaagca cagtttctgg aaatccaaaa gatattcatg
    18961 aagatccaat ccgcttggga agaagaaact cctgaaaaag ccagaagctg ttatacagac
    19021 aagctgtttg ccgaacatca gaaagttctg caaaaaaaca aagcagacgg tgtaagaaat
    19081 catactaaaa aaataactat tcaggaagta ggaaattatc gacagatcca cgaaaatagt
    19141 tttgccatcc gaattgattt cagatgctgt gattattcag tcgatcgaaa agatgggcga
    19201 ttgatctcag gaagtaagac acaacgacag tatttttctc agatatggta ttttgatttt
    19261 tctgagaaag aaaaaagatg gcaagccgat tttattcagc cgattatgct ggattgacag
    19321 gaaatattta tgaacggagt tgtgacaaaa gccttgtcgc agctcctttt tctgaataaa
    19381 cggtgtgcca gagatagcct atcggcaata aggcacgatt ttgtaaaata tgggaagcga
    19441 tttttcaaaa tcgcttctta tcgctcaagg ctgaacatct ctgtcacaac ctctccacta
    19501 aatggtgttt aaaagcttgc atctgcgata ataagaacaa ccaaacgaaa aataggaagc
    19561 actattttag tttagttgtt cttattactt gatgcaggac agctttttca caacctcttt
    19621 tttgtataga tgggcaaaat ttttgtaata ataggcgtat gtctttttcg ttgaggagat
    19681 gaaaaagcat atggcttatt ttttgtttgc tttgcttact ttctttccac ttcaagtggc
    19741 agaaatatct actttttatt atgtaagcgg aaccatatac ttgaaatcag aaggagatca
    19801 tttctatgaa aacaagaaca attgaaatac ttcacagaat catgacagcc caatatccag
    19861 taaaagcaaa acatctagca agtgagttca atgtcagtca gcggacgatc cgtcaggaag
    19921 ttcttgaagc gaatatctgg ttgagcagta aaaagcttcc ggagatccgt acaattcgca
    19981 acaaaggttt tttgttgaag ctggaaaagg aaaagcaagc tcgattagaa actgaaataa
    20041 aagatgtgaa agacggcttt ttgaatcggc gcgaacgtat ttttgatcta gttttagccg
    20101 ttgcttacag ccgaatcccg atccatctaa acaaaaaaga agaagaattt caaatctcta
    20161 aaagtacatt agatgaagat atgagacggt tacgagcaac actggtcagt tatgggatag
    20221 agatcgtcag ttatggaaag caaggactgg tttataaagg ttcagaacga tctatccgta
    20281 cgatgatcta tgattttatc aatcagaatc tgggaatgac cgacttcttt atgaatcaag
    20341 atgcacaatt aacaccggct caaaaaatct ttctccatta ttttcctttg gtggaaatca
    20401 aaaaaataca ggagatctat tccaataagt tgttgaaaca agaagatgat atttataaaa
    20461 aacaaatcct gctttttaca ttgatctgga tccagcgaat caggaaacat gaatttatct
    20521 ctgccattaa ttggaaaaat acagaaataa aagacagtag cttttctgca tttattgacc
    20581 acatgatcga ggaatttcaa ttggacaatg tcccgcaagt agaaagaaat tatcttcgtt
    20641 tcactattga gacgttcaat gctagagata tctccaattc aatcgagtgg gtacaagctc
    20701 aattactaac gatccaattg atccaatttg tagagaacga gacacacgta cctttccatc
    20761 taaaggaaga agctttattc gaaaatctct ataagcacat ggctgcactg attgtaagaa
    20821 tcaagaatca tatccaagtc atgaatccat tgaaagaaaa catccgaaca aattatttcc
    20881 caatctaccg cgcagtcact cattttgttc ctacgatcga agaagtaatt ggtggcaaga
    20941 tactggagga cgagatagct tttcttgtca tccacttttc gacgatcgcc agtgtaatca
    21001 agcgagactt gaattatgtt tataaatcag tcgttgtctg taaccatggg atggctactg
    21061 gaaatctgtt agcagagaat ctgaaagaaa agtttcctca gatcgagatt actgcagttt
    21121 taagttcaaa agaaatcgaa ttagtagata aattggatgt caatctggtt ttcagcacct
    21181 ttcaattgaa ttatcgaaat aagccgcttt tagttgtcga tccgatttta actgagacga
    21241 atatcttggt tatagaagac tttttgaaaa gaaatgaaga actacagcgg ttagagccta
    21301 acagtcatga atcgacggag ttattcactc aaataataga tgtcatgaaa gaaagtggtg
    21361 gccgagtaga tcgaatcatc tatggcaagt tagaaaaatt attcgaaaca aacaatttag
    21421 aaatcaacaa aagggagatc cagcctatgt taaaagatat tttgacggac agcaatatca
    21481 tgatcaaaga aaaagccact acttgggagg aatcgattga actagctgct agaccattgg
    21541 ttaaagaaaa cattgtcgaa gagcgctatg tcgaagcgat gattcgtgca gtgaatgatt
    21601 acggaccgta tatcgtgatt ggcaaacaca tggcactagc ccatgcccga ccagaagatg
    21661 gggtcaataa attaggagtc agcgtagcta cgattgagca accaatcgat ttcggtaatc
    21721 ctgaaatgga tccagtaaag attatttttt gtttggcagc agtagactcc tactcgcatc
    21781 tgaacatcat gaaagaacta attgaattga tcaacaacga agagaagttg gatcgattga
    21841 ttgaatgcac aaatatcgaa gatttcaaac aactgctttt ttcagtagtt gagaaataat
    21901 aaatgatacg ggggagaaca aagatgaaca aattgaacat tttattcgta tgcggtgcag
    21961 gtttaggaag tagttttgca gcacagatgt ctgctgagga tgttttgaac aaattaggag
    22021 tagaagccaa actggaccat tgtgatattt cttccgcagt ttccatgaaa ccagatgtga
    22081 tcatcacagc agaaaatttt cgttcgcagt tcgaaaagtt cgcagttgat cctaaaacgg
    22141 cgatgatcta tttgaaaaat attgtttcaa aaaatgaaat tgaagaaaaa atcaccccag
    22201 ttctgcaaga taagggagtt atctaaatac aaaaagaaaa gagggtaaaa a
//
LOCUS       DAZKOW010000043        22218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000043 DAZKOW010000000
VERSION     DAZKOW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.043"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..485)
                     /gene="yajC"
                     /locus_tag="ACG912_001534"
     CDS             complement(105..485)
                     /gene="yajC"
                     /locus_tag="ACG912_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFQ0512862.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(582..1727)
                     /gene="tgt"
                     /locus_tag="ACG912_001535"
     CDS             complement(582..1727)
                     /gene="tgt"
                     /locus_tag="ACG912_001535"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFQ0512863.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1945..2643
                     /locus_tag="ACG912_001536"
     CDS             1945..2643
                     /locus_tag="ACG912_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0512864.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2811..5450)
                     /locus_tag="ACG912_001537"
     CDS             complement(2811..5450)
                     /locus_tag="ACG912_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0512865.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6069..7100)
                     /gene="queA"
                     /locus_tag="ACG912_001538"
     CDS             complement(6069..7100)
                     /gene="queA"
                     /locus_tag="ACG912_001538"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFQ0512866.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7293..8467
                     /locus_tag="ACG912_001539"
                     /pseudo
     CDS             7293..8467
                     /locus_tag="ACG912_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
     gene            8471..9112
                     /locus_tag="ACG912_001540"
     CDS             8471..9112
                     /locus_tag="ACG912_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0512867.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9109..10026
                     /locus_tag="ACG912_001541"
                     /pseudo
     CDS             9109..10026
                     /locus_tag="ACG912_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
     gene            10023..10694
                     /locus_tag="ACG912_001542"
     CDS             10023..10694
                     /locus_tag="ACG912_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0512868.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11235..11723)
                     /locus_tag="ACG912_001543"
     CDS             complement(11235..11723)
                     /locus_tag="ACG912_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFQ0512869.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11924..12190)
                     /locus_tag="ACG912_001544"
     CDS             complement(11924..12190)
                     /locus_tag="ACG912_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0512870.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12190..12453)
                     /locus_tag="ACG912_001545"
     CDS             complement(12190..12453)
                     /locus_tag="ACG912_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0512871.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12974..13114
                     /locus_tag="ACG912_001546"
                     /pseudo
     CDS             12974..13114
                     /locus_tag="ACG912_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13256..15079)
                     /locus_tag="ACG912_001547"
     CDS             complement(13256..15079)
                     /locus_tag="ACG912_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0512872.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15677..18382)
                     /locus_tag="ACG912_001548"
     CDS             complement(15677..18382)
                     /locus_tag="ACG912_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFQ0512873.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18688..19635)
                     /locus_tag="ACG912_001549"
     CDS             complement(18688..19635)
                     /locus_tag="ACG912_001549"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFQ0512874.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19825..20094
                     /gene="rpsN"
                     /locus_tag="ACG912_001550"
     CDS             19825..20094
                     /gene="rpsN"
                     /locus_tag="ACG912_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0512875.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20270..20632)
                     /locus_tag="ACG912_001551"
     CDS             complement(20270..20632)
                     /locus_tag="ACG912_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0512876.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20643..21764)
                     /gene="alr"
                     /locus_tag="ACG912_001552"
     CDS             complement(20643..21764)
                     /gene="alr"
                     /locus_tag="ACG912_001552"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFQ0512877.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21780..22130)
                     /gene="acpS"
                     /locus_tag="ACG912_001553"
     CDS             complement(21780..22130)
                     /gene="acpS"
                     /locus_tag="ACG912_001553"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFQ0512878.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
BASE COUNT         6286 a         4757 c         4141 g         7034 t
ORIGIN      
        1 aatattgagc aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta
       61 ggacatcttt ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc
      121 tcttctgtct cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt
      181 tcttctgttg tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc
      241 gttttgattg ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc
      301 cgtttgtcat tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa
      361 tcacctggtt tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa
      421 cgtgacataa aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt
      481 cccatttttt tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct
      541 gataacacta gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt
      601 tctttattga acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc
      661 atgattgctt gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa
      721 gtcaaacgaa tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta
      781 tagtttttac atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat
      841 ttcgcatttt tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt
      901 cgagtaggca agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa
      961 tcagcagcac cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg
     1021 aattctaata cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag
     1081 taaccaggga aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac
     1141 cctgctcctt ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat
     1201 cctcgttctg cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca
     1261 tcgaatggtg gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg
     1321 ttgattgctt tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc
     1381 acgccttctt cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa
     1441 tctgttaaaa tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact
     1501 aagtcttcac cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc
     1561 atttctttca actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg
     1621 aacattggtg tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta
     1681 tgtttttctt tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact
     1741 ttctatataa aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa
     1801 gttccctcgg cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat
     1861 aacaaaaaaa agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt
     1921 attaaagaaa aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa
     1981 gagagtctaa aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt
     2041 ggtattgcta ctattcttct tattatgatt cctattatct tgtttgccac caccatatat
     2101 gatggatcag cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt
     2161 ggcggaatta tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat
     2221 cttgatattt tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga
     2281 ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca
     2341 acattatggg gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa
     2401 agctatttta tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat
     2461 acgatcactg caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat
     2521 ctttcattta tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg
     2581 ctcagcccat acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt
     2641 taaaagagag tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc
     2701 agtaaaagaa acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc
     2761 tcctttttgt atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct
     2821 tcttctgctt ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata
     2881 gataaaatcc ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct
     2941 tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct
     3001 accaaagctt tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg
     3061 aacacatgca gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc
     3121 tgacttgcgt ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac
     3181 aaggttacga tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca
     3241 aagatccctt catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct
     3301 cttcctaaag caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca
     3361 gctgtaaatg gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa
     3421 atattacagc ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct
     3481 tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca
     3541 ctttgcgcca catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac
     3601 gctggtgcat cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa
     3661 gatttgacga tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg
     3721 acacgcgcat ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct
     3781 gacggatcat tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct
     3841 ccagtgatca tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt
     3901 tctggacgtg gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt
     3961 gcttcagaag aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact
     4021 ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa
     4081 ctaaagcgcg gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc
     4141 atttggtgaa ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt
     4201 ggatattttt cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg
     4261 gctacttctg ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca
     4321 tctgtacata gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg
     4381 atattcgtta cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc
     4441 agcgttcctg ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc
     4501 aactgtcgga tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac
     4561 gtaacaataa cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg
     4621 aacatctcaa ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc
     4681 gctaaagcga tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt
     4741 ttttgcatag tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca
     4801 atggcagtga cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata
     4861 ttaagttggt cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact
     4921 gcttcacttt ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa
     4981 cgggcatcgg cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt
     5041 tgctctgact caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc
     5101 atatcttgca aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc
     5161 gagttgatca caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta
     5221 gcaaatgcgg tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg
     5281 gacaagcttc gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc
     5341 aatccctgct tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct
     5401 gcttctgtta ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc
     5461 ctttaaaatc ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta
     5521 cttcaaataa agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata
     5581 tttgcaaacg ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa
     5641 tagacagaag aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt
     5701 ttcacccaga catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg
     5761 gatcacgaag tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg
     5821 tctgaaaatt gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa
     5881 tttttgttta acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa
     5941 tcatttctgt ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg
     6001 gctttttctt tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt
     6061 tcataacctt atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca
     6121 atcgcatgct ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg
     6181 actaaagtcg attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca
     6241 tagccaggcg taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat
     6301 ttcgttccaa tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg
     6361 ccatttttac gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca
     6421 ctatgcatat ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct
     6481 acatgtagtg tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct
     6541 ttcgtaaaat gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca
     6601 gtttgatagc gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct
     6661 cccaatgatt caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg
     6721 ccgtgttcca attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt
     6781 gtcccaactt tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta
     6841 ttcgttagaa gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga
     6901 gcaggcagaa ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg
     6961 atttcatgga aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga
     7021 gaatgatcgc ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca
     7081 aaatcttctg ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc
     7141 atgtttttcg ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa
     7201 ataaaaaaac taccctattt gtattttttc attgttttag gcatactagt aatagatagt
     7261 tcttcattga aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa
     7321 tttataaagg tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt
     7381 tcatttgttt tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc
     7441 gaatgactga tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg
     7501 atccagtgga attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc
     7561 acatgacgat ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag
     7621 aacggaataa aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag
     7681 atcgctaccc acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc
     7741 ttgcagcgaa tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca
     7801 ctcgtgattc cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg
     7861 tatttgtaac ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga
     7921 gtgaaggccg tgtgatcaat ttgatacacc agaaaatatt ttacgtcatc ctgcaaacga
     7981 tttcgtagaa gagttgattg gagaagatcg cctactacaa gccaagccaa atgcgacaag
     8041 agtcggcgaa gttatgctaa atacagcaat cacgattaca ccggaaaaat cattacaaga
     8101 agcaatcaaa ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg ataattcgaa
     8161 tgtattaaaa ggatttatcg atgtagaaac gttgaataaa aaacgaggaa aagtatccag
     8221 tgtcggcgac attttgaata aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga
     8281 taccttgcaa cgcattttaa aacgcgggct gaaatacgtg ccggtcgttg atgaacagaa
     8341 caaagtcgta ggtatcctga ctcgtgcatc attagtggat atcgtctacg atgtgatttg
     8401 gggagaagat cccgcaacta ctgatgttgc agagtcagct actcctgaag aatctaagga
     8461 ggtctgacct atgcagcagt tttttcatca gcacggttca gaaattctct caaaaggttt
     8521 tgaacatctt tatatttcag gacttgcttt gatcattggt atcatcattg ctgtaccgct
     8581 tggtgtctta ctgacacgtt ttccaaaagc tgccacgatt gtgattggct taaccagtgc
     8641 cttgcagaca atcccttctc ttgctttatt ggcattgatg atcccgctat ttggcgtagg
     8701 taaattacct gcgattatcg cactctttat ttattcatta ttgccgattt tacgtaatac
     8761 gtacattggg atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag gcatggggat
     8821 gactgagttc caatccattt tttccgttga attgcccatt gctatgccaa cgatcatggc
     8881 tggtattcgt ttagctgccg tttacgtgat tgcatgggct acacttgcct cttatatcgg
     8941 cgctggtggt ttaggcgatt tgatttttag tggactgaat aattaccaac cagatctcat
     9001 tttggcaggt acgatccctg taacaatttt agcactattg gctgattggc ttctaggtct
     9061 cttggaacac cggctgacac cgatcgcact tagagaggag aatgaagaat gaaacgtata
     9121 ctaaaaatga ccatggttct tttctctctt cttatacttg cgagttgttc cttccctggt
     9181 cttgccagca atacagatga agacaccatt tcgatcacag gtggaatcac ttcagaagca
     9241 cagatcttag caagtctagt tgccggaatg gtttaacatt atacagataa aaataccgca
     9301 atcatcaaca atctggctac tacaactatc aaccatcaag cgatgatgaa cggagatgct
     9361 tcaatttcag cggctcgcta cacaggaaca gatttaacga ctacattgaa ccttcctcct
     9421 gaaaaagatc cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa aagatacggt
     9481 caaacgtggt tcccgtccta tagttttgaa aatacgtatg tctttcttgt tcgaaaagac
     9541 accgcacaaa aataccatct atctaaagtc agcgatctga aaaatgtagc ggatgaatta
     9601 gtagctggtg tagatacttc ttggatcaat cgtaaaggag acggttatga cggcttccaa
     9661 gaaacttatg gcttctcctt caattcgata ttacctatgc agattggact tgtttatgac
     9721 gcagtcgaag caggaaaaat ggatattgtt cttggctact ctacggatgg acggatcgcc
     9781 agttatgatc tagtcatgtt ggaagatgat cgtcaattct tcccaccata tgatgctgct
     9841 ccagttgtag acaacaagat attagctgat acgcctcatt taaaagaagc cttaaataag
     9901 ttaggtcata ccatcagtac ggaaaaaatg caggaattga actacgaagc agataataat
     9961 ctagtggaac catcagtagt agcacaacgt ttcttacaag aaaaccacta ttttgaaggg
    10021 aagtgaagca attggataaa atgaatgtct ttcaacaatt tttctattat ttccaggaaa
    10081 atggctccta tatctttgca caatttatcc gtcacttcct gatttctatc tatggtgtcc
    10141 tctttgcagc agttgtcggg attccggttg ggatcatgat ttctaggaga cgtaaattag
    10201 caaactgggt gattcgtttg gcgaatatca tccagacgat cccttctcta gccatgatct
    10261 ccattttaat cattggatta ggcctgggag tcaatgtcgt gattgttact gtatttttgt
    10321 attccctctt accgatcatc aagaatacgt ataccgggat gatccaagta gacaaaaaca
    10381 tccttgatgt cggcaaaggc atggggatga ctgctagaca acgactattc atggtagagt
    10441 taccactctc tgtttctgtt atcatggcag gcataagaaa cgcgttagtt gttgccatcg
    10501 ggataaccgc gatcggtgca ttcgtcggtg ctggtggtct tggagatatc attatccgag
    10561 gaaccaatgc aacagagggc acatcgatta ttttagcagg tgcattgcca actgcattaa
    10621 tggcgatcat cacggactgg cttttaggta ttttggaaag acggctagat ccagccagcc
    10681 gtacatcacg ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca
    10741 aaggcttttt tagttctgtc atttttaagg caataatcca ctctgattct tccctcctta
    10801 tgggcaaaga gttgcttttc ttagctattt atccattccg tatactccaa cttcatactg
    10861 ccaacgggtt catatctgca tccaccgact aagttttttt cttatccgct aaggcatttt
    10921 gccaatccat tttcccatca ctcgtcatgg aattcatacc acatgatctc taaatactcg
    10981 tctgcttgtt ctttgaattg ggcatcaatt cttacatcct ttggcgtggc tttcatttaa
    11041 gccttttcca tcagtcctcg attgttcgat gccttcagct gcatcatcaa aactggtgac
    11101 tgccgtatcg gtaacttcga tcatctttta tacaatcggg aacttgccat cctcatcctt
    11161 ggctaaaaac atatctagcc attccagctt ctcgccatgg gaggtttgcg gttcctaaaa
    11221 ccggtacacg attcttatgg actgcatccg tcgcaactag acttggcgat acgatgatcc
    11281 ttcaccagaa gagcaccccc gtaaaccaat tgatcgatat attgccaata agaaaagaca
    11341 ttggcgtaaa agatatcgat ccctcgtgat gcattgccgt tgatactgct attcatggcc
    11401 gaaagcgcca cgactttaac ttcttccgtt tgagtgacat tcatgttcgg caatgtcgct
    11461 ttggataccc gctctgctaa aggaacgaca ctggtattgt atttcgtatc tgtatctttt
    11521 ccaaaagacc atgccaacga atcctctggg aaccaataag acgactccgg ttgattgtcc
    11581 attgcttttt tcaacatact tttggctgtt tctcgataaa cgacatttcc aattgtcttc
    11641 gcctcacttt ggcgataatc gatggtcgtt gccactgcca acactaacaa cgatgctcca
    11701 attttactga ttttccttgt cacacttctt cctccttgtt ttacataaac ccgtcaacat
    11761 tccattccta aactcaaaca atgacccttc aaaggtggag atataggaac aaattaaaga
    11821 aatcatgcaa taaatccaag aaggttggtt ttagaacaaa aaaagagtct cctttttggg
    11881 gagactcttt gttaactatc taaaaaggtt ggctcttctc acttcactca taatgtgtcc
    11941 aggcagaata aatttctaca actttatttt cctcatcaac tttgtaaacc actcgatgct
    12001 gtctattcag cctgcgtgag tatctgcctt catgtgtagg tctcagcttt tcaaaagact
    12061 gcgtgggcat atatggatct tccttcaatg tctgaatcac ttcttcaaat tgtttcttta
    12121 aattcgtttg cttgattttt cttaaatcca ctttcgcaga gtttttgatt gcgactgtat
    12181 agttactcat caaagagctt cccaatcaat atcatcgata ttcgtaaagc tactgtcgtc
    12241 tttttcgcgg tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa gggtttcttg
    12301 aatcgctctc cagtcttcca gcccaatcaa tacagcattg tttccactac gatcactaat
    12361 aatctcgatt tcagtatgat tttcatttac ctctttcaat aactgataaa aatttttacg
    12421 agcagtgcta ggattcatcg ttgtattagc cattgtctct cctccttttt ctgatataat
    12481 tatagcgtac ttgattaagt acgtcaaaaa aatacaataa agcacttaaa ttcagcaaac
    12541 ttttcctctt ttttacatag agaaaaagac ccctttttag ggaggatctt tgtccacgct
    12601 cacttcttac tcctttacga ctagcggtag ccaaacgtaa agtaattggt aacaacgtat
    12661 catctttagc aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac caatacacga
    12721 aggttcctcg ttgcagtgtg acttgattga tatcttgaat aacctgagga tacgcacgaa
    12781 agatttgtaa cggttattca aaactgattt tggccgtcaa gtcttctact gcctcaaaaa
    12841 tgattgcctc tttcgtctcc tgtcattcat gttcactatc gatttgatac ttgcttagac
    12901 cgttgggttt gtgaatcacc aattaaaaat ggcactgagt tatggtaaca aaaaatggtc
    12961 acttgacgag gctgagattc ctacttatga agacacatgg cacttaagag tcgaggtaaa
    13021 ggaagaagct gaaaatatag gaggctgtac attatttggc aaaggattcg gaaattatga
    13081 tcaagacatc aaaatcaaaa tgttttacag ctaaatagga agaataataa catttcatac
    13141 gctgttccca tctttaatgg cttatagagg gcacctttag ctgagaataa gacaaaacag
    13201 accctctagc ttcaattaag agctagaggg tctgtttttg attttaaaac ttggtttatt
    13261 tcttcaaatg gtagctatag gtgctgacaa cgatattttc acgccaagac aatcgcaatc
    13321 ttaaccgcaa acttgtttgg ttatggagaa tattctgcca gcgtctgttt ggtacaaatt
    13381 gaggaatcaa cacagtagtt gtatagtttt gtttcgctgc atttttactt acgatatcta
    13441 cataacggat aattggattt tcaatcgagc gataagaaga gtggacgatt gaaaaacgga
    13501 catctgggaa atgcttttta aattctgctt cgatttcttt ttctttttcg acatcttcgt
    13561 ctagcgaaac atgcatcgca acgacataat caccgatcga acgtgcgtaa ttcaacgcac
    13621 cgatatttac ccgtgtcaca ttcccgacta atacaagaac ggtattcccg tcataatcat
    13681 gtagttttgc atcattttct aaacgaagct gttctgctac ttttttataa tgatcatgga
    13741 tcttataaaa aatgaacata acgattggca taatgatgaa gaaaggccaa atgtccccta
    13801 accgatagac aaacaaaatc gcaatgattg catatgagat gaacgcaccc gtaatattgg
    13861 caatcgattt gcttaaccat ctttttcctt cttttttcca tttgacgacc atccctgttt
    13921 gagataaagc aaatggaatg aacacgccga tagagtataa cggaatcaag cgttctgttg
    13981 agccatggaa aataaacagc aatacgatcg aacctagcgc tagtgtgata atcccattgg
    14041 aatagcctag tcgatcccca cgatcttgat acatgtgagg catgaattta tcttttgcca
    14101 gattgtatgc aagaactgga aaagcagaaa aaccggtgtt agcagcaact gctaaaatca
    14161 gcgctgtagc aaattgaagg acgtaataca aaataccatg tccaaagaca gctttaccaa
    14221 tttgagacaa aacagtgact tctttttcag gaacgatccc ataccaatag ttgataaatg
    14281 tgatccccac gaagaagaaa cctaagatca atgccatcat cgtcaatgta gcagcggcat
    14341 tttttgctct tggcttctta aagaatggta cagcattact aatcgcctcc accccagtta
    14401 aagaggaaga accagaagaa aatgctcgta aaatcaaagc gatagaaaca cccggcacag
    14461 ccgctcctgg aagtgcagta gcttgcaatg gcacagcacc tgtcacgata ttatataaac
    14521 caacaacgat cagtaacgag atcacaagga taaatgtgta caccggaaat aataggaacg
    14581 atgcagattc cctcagccca cgaagattca gcatcatcag tagcaaaacg attgtcacag
    14641 aaatagcgac ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct tcagcacctg
    14701 ctgagacaga tactgctact gtcaacatgt agtcgatcaa taaagagcca ccggaaatca
    14761 agccagcatt ctttccaaga ttctcactgc tgacgacata tgctcccccg ccatgaggat
    14821 aagcatggat gatttgacga tatgatagcg ttaatgaaat caataaaata ataacaaaag
    14881 cggcaatggg tagcgaatac cagatggctg cagcagacaa agcgactaaa acgacaacga
    14941 tttgttccgt accatatgca atagatgata acgcatcaga cgacaataat gccaaagcag
    15001 caaatcgggt taatttgtgc tcatcatttt cagcagattt taaaggctta cctactaaca
    15061 acctttttaa gtaatccaat ttctaaactc ctttacacac aattttactt caagttttat
    15121 agtcgaaaat ttatagtaaa caagatgtta tagtacgctc actgacctta aaagtcaata
    15181 aactttaaga aaataattgg aaaaaaacag aaaaatatca agcgcttttt caaacgcttt
    15241 aatacagcaa aaatctaaca tttttttatg ggctttttct ttgaattcta taataaaagg
    15301 atagaacttg acttagaaaa tcaagttcta tcctttttga tatcttaact tttggaagag
    15361 agatgtaagg ctgctttcta cccatcattc ttactactcg aagcatagcg cagttgtcac
    15421 aacctcttga taaacggtgt ttaaaagctt gcatctgcgg taataagccc aaccaaatta
    15481 aaaacataag aagctgtttt tatttggtcg ttcttattac ttgatgcaga gcagcttttt
    15541 tacaacctct tggtacacgg tgttcaatca gctgaccctc ggtattagct caaaaaaacg
    15601 gcaaaatatg aggaactatt tttccatttt tttctctaat actcaggtca aaacactgat
    15661 ttcacaacct ctttttttaa tgaaaaatat ggctctcatg cgccgcttct ttgaatttcg
    15721 tcattttttc ttcgatgaac ggatagattg ccgttcctac gattccaaag acaacaaaca
    15781 aaccgatcat gatccagatt gctttcgtag catttggcca ataaattcct cctgcagatt
    15841 cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg attgatcatt tggaagaatt
    15901 tccctgagac ttggatcgga tagttcccgc ctccgccaga gatcgacagt actaagatga
    15961 taatagcgat ccctttaccg acattatcga ataatgcagc caagacatag acgatcatca
    16021 tgaacgctaa ggcgatcaag acagcaaatg acacgctgta gaccggattt tggacatcta
    16081 ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc gccattgtca
    16141 aaaatgtcag catacgagca acgaattgtt ctcgtttcgt aaaattgttt cggtctttct
    16201 ttcctaaata gtattctgtc gtagcaacac ttgaaagaag aacagcgccg acccacagac
    16261 atagtgcagt ataaaacggg gtgctagctg aaccgttatt tgcgattgga taaagcgcat
    16321 tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg tctagtttca
    16381 ataatttgat gacttcgcct agatcgactt cttgttcgcc ttttcggatc gcgtctgctg
    16441 ctttttggat accagctttg atatttggcc agtcattgtt gatcaagtcc gcagcagcgt
    16501 tcacagctgt ttcgacttct ggcatttttt catttactgt tgtcaatgta ccggtgattt
    16561 cattttttag tgctggccaa tcattttgga taaaatcagc ggccaatcct aatttttgct
    16621 gaatgatcgg taattcattg ttgtataggt ctgccccttc attgatgcca ttgacgatgg
    16681 tatccatatt tccattcagc attgtattgg catcatggat ttcttgtcca attgcaggca
    16741 tttctttttg atatttttct aaaattgcca ctgcattcgt tacagttgct ttcgttgagc
    16801 tgagaagcga atcaaagtcg atctggtccg ctttgctcag tgcgccttgt gcagtcccaa
    16861 tcgtatcgat gacttggtct agtagagaag tgacagtgga actgatttga cctgtattga
    16921 taccagcaat gatgccattg atattattaa caacggcatc aatttgatct agcaattcat
    16981 caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg cggttttgca
    17041 gattgcttag caagggtttt aattgattta gctgatcaat aattggctgt aatttcgtat
    17101 ttccagcact tgtttgcaat ttagtcagga actcgaccag tttatcgatt gcattttgtt
    17161 gttgagatat tccgtcaata aagccgttca atacattgct gatgtttttc ttttcatcat
    17221 cagtcagctt accatcttca atagctgttc ttacagtatc tattaacgaa cctgtcgttg
    17281 ttgccacttg tccaattgac ttcaatgtaa catctacact gtttgtgatg cttggtatcg
    17341 catctttcag ctgagaggca cctgattttg tttggtttgc tagatcactt gcctgatttt
    17401 ctaaattttt cacatctggc aagatatctt gtacttgttg gatgatcgtc aagccttgtt
    17461 ttgcttcatc gattccttgg ttcattgtct cttggatcga agcaaaatcg ccgtcgatct
    17521 ctgctagttg cttaccggca ttttggatct ctgggatttt ttcttgcaaa tcaaggatga
    17581 tttttgcttg atctttcaac tcaggcattt tgtcattcaa tgcaacgact ttttcaccca
    17641 tctcatctac tttagggatg taatccacga attcatttgc tttattcagt ttttccttga
    17701 tttcaggtag ttgactttgt aattcgagga cttgttttgt gtagccgtcg atcgtgtcca
    17761 aattttcatc tgttgataaa atcatatctt taactttatt gatacttact aggttcgtat
    17821 cgatgtcgta acctatctca ttgaatactt tcaataaagt cgaactggct gttttgataa
    17881 attcatttgt gatttgcgat tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa
    17941 tcgcattgat tttttcattt accgtatatt cgatttttgg tttcttgata tcaccactag
    18001 taaaactcag cagatcttct gagaaatcct ttggcaagta aatacctgcg taatactttc
    18061 cggaacggac tccgtcttct aattgttctt ttgaatcaac gaattgccat cctaattgtt
    18121 tatttttatg aagcgattcg atgacttgct ccccaatggc cacttcttta ccttgaaact
    18181 ctgctggctt gtctgcactg taaacagcaa ttggcagctc tccagtattt ccatatggat
    18241 cccataatgc tttgatgtta aaccacgcat atagcgaagg gatgatcatg atcgcgatga
    18301 tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa
    18361 gcgtattttt gattgactgc atagttctcc cccttcttct tttttcagct gaattacttt
    18421 accatgtatt tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat
    18481 atcacggcaa ttgtctcatt gtaatctctt acatattgag aaataattaa ggattacagc
    18541 ctttacttta tcatgaaata aaagggaaag ataattttat gccaaagttt ttatctatta
    18601 tacgtatacg caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc
    18661 ttttttcgtt ttttattcct gtatttctta tttttcgaat gacaggatga ttttcccagc
    18721 aggaggatgt ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg aagcagacag
    18781 attcattgaa ataaaaacat gaaaggctcc tttttggtag gctttgatta tgccagcaaa
    18841 ggcttctgca tctagatatt cctttcttgg aacaaacgat tcataaaagc cttccttttt
    18901 ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca tacctgccgc ctggtttcat
    18961 gatctggatg ccggtttctc ctttgatact tcctttcgtc gcatcgatta caaggtctgc
    19021 ctgatcagca aattgcagac ctggatttgt tttatcataa gcagcaaatg ctgaagctcc
    19081 taattttttt accttttcct catttttact ggatgcactg gttaagattc ggattccctt
    19141 ttcatggaga agctggatca gacttgaccc gaccgcacct gatgctccta gtatcattac
    19201 agtatctgtc ggctggattt ccgtcaaatg gttgattaga ttataagccg taattcctgg
    19261 cgtgaccatt ccggctgctt cttcccaact cattttatct ggaatttttg ctacttttgc
    19321 tgatggcaac acgacttctt ctccgtaagt cccgccgact gcatggacag ctactcgatc
    19381 acctacatga acagttgtga catctgaacc aatctctgtc acaataccag cgccatcatt
    19441 acctggtaca taagggaatt tcaatgttcg tatctctttc atttctccta aacgcaaggc
    19501 aacatcataa ggattcacac tgaatgcttt tatttctaca cgaacatgtc cagaaccgac
    19561 ttctctggga tgagcatcga tttcttcaaa aacatcttgt gcttctccgt aaccgttaat
    19621 tgcaaatctt cgcatggtca ccatctccct ttttctttaa gtttacgttt atgcctttat
    19681 tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt
    19741 tttcgcgtaa cgtgttaaat tatttatcta aaagcgtaaa gattacgatt tacgctaata
    19801 aaaaataatt ggaggaattt gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa
    19861 cagcaagaaa cgattgaacg ctatgctgct ttacgccagg aattaaaagc gcaaaaagat
    19921 tacgaaggct tgcgtaaact tcctttagat gctcgtccag aacgattgaa aaatcgtgat
    19981 ttaatcgatg gacgccctcg cggatacatg cgtaaattcg ggatgtcccg tataaaattc
    20041 cgtgaattag cacataaagg tttgattcca ggagtaaaaa aagccagctg gtaatccaag
    20101 tctggctgta atgtaaagga ggagggcgca tggaccactc cttggatcat caaaaaagat
    20161 acgttcgctt cttctagcag aaatgctggt actagaagca gacgtatctt tttttacgca
    20221 cagctggtcc ttttttatat gtaaaggctg catggttact tcgctatatt tatttagtaa
    20281 caggagtcag tgcttcgata cccacactaa ttttcaatgc tcggtcaact tctgccataa
    20341 tgggtcgatc caaatggcag accttttctt tcaatcttga tttatcgatc gttctgatct
    20401 gctccaacag gataacggag tctctttcta ttcctgttcc agtagctttg atcccaatgt
    20461 gagtcggaag cttaggttta gccatttttg ccgttatggc cgcaacaatg atcgtcgggc
    20521 taaaatggtt acctagatta ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg
    20581 atcccacgac tggagaaaga tcagcaaaat aaatatctcc tctttttacc ataggatcct
    20641 ccttattctt gatattctct tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg
    20701 atcgtaccta attgatctgc tatatcctgc atagtgattt ctttgtctgc atttttgccg
    20761 ataagcgtca cttttgttcc gacaggaaat tcttgaggca atcggatcat cagttgatcc
    20821 atacatacac gcccaatcgt ttcacagtag ttcccttcta ccaatagaga aaaaccctgc
    20881 atctttcgtg gccagccatc tgcataaccg atcggtatcg ttccgatcca ctctgcttca
    20941 ggagtgatat atgtttctcc atagccgatt ccttcacctg ctgggagttt tttgacttga
    21001 atcaattcag aaaccagctc aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc
    21061 ccagagggat taagtccgta cattgctact ccataacgaa tcatattgcc gattgtttca
    21121 tcatgccata aagccgttgc actattgctg acatgtacat atcgaggaag ttcgggaagt
    21181 tttttcagca cttctataaa gcgttctttt tgcagattcc aatatgtatc atctgcctga
    21241 tctgctgtag aaaaatgggt aaataaacct tcccattcta atacacgact ttctttgatg
    21301 aatgcagctg cctcttttac ttcttcaggt gtacaaaatc cgatacgacc catcccagtg
    21361 tctgctttta gatgaatcga aagaggcgtc tctagcttac ttttctctaa aacagcagct
    21421 gctgcttgca accattcccg tgtacctgca gttactgata aatcatgagc aacagctaca
    21481 gtcagtgcgt cgtaaggaac catattcaag ataaggatgg gctgagtaaa tccagcttct
    21541 ctcaactcga ctccttcatc taaatttgaa acacaaaagc ccgaagctcc acctgcaact
    21601 gctgcctcag ctgtttcaat agctccatga ccgtatccgt tcgcttttac tactgcaaac
    21661 aattcttttc cttgcggaag tcttgcaact tcatttgcta cattttcagt tatagctttt
    21721 ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt tccttctttc
    21781 tagtcttcca aaataatttg tgcaaaagct gtttcatttg tatgagtaat actgacccat
    21841 acattgcctt catgaggtga acgcgtcaca acaggctgac cattttcatt ttttaaaatc
    21901 tcgacatctt gaaaggtaac ttttccgatc ccagttcccc aagctttaga gaaagcctct
    21961 ttgcaagcgt aacgcccgcc taaaaattct acttggcgat gaaaaggcaa ggactggaat
    22021 agtttcattt catcagaggt caagattcgt gcaatgaatt ttggtttttc ttcgatcagc
    22081 cgagtgatcc taggaagttc gactgcatcg atcccaattc cttttatcat atgcttttct
    22141 cttttcaata aatttagaac actctcattc taccaaatta taaccatttt gccacaaata
    22201 agtccacaaa taacaaaa
//
LOCUS       DAZKOW010000044        22123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000044 DAZKOW010000000
VERSION     DAZKOW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.044"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(317..1813)
                     /gene="lysS"
                     /locus_tag="ACG912_001554"
     CDS             complement(317..1813)
                     /gene="lysS"
                     /locus_tag="ACG912_001554"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFQ0512879.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="ACG912_001555"
     CDS             complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="ACG912_001555"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFQ0512880.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="ACG912_001556"
     CDS             complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="ACG912_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFQ0512881.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="ACG912_001557"
     CDS             complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="ACG912_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFQ0512882.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="ACG912_001558"
     CDS             complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="ACG912_001558"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFQ0512883.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="ACG912_001559"
     CDS             complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="ACG912_001559"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFQ0512884.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8344..8814)
                     /locus_tag="ACG912_001560"
     CDS             complement(8344..8814)
                     /locus_tag="ACG912_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFQ0512885.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8860..9285)
                     /locus_tag="ACG912_001561"
     CDS             complement(8860..9285)
                     /locus_tag="ACG912_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFQ0512886.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9352..9621)
                     /locus_tag="ACG912_001562"
     CDS             complement(9352..9621)
                     /locus_tag="ACG912_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFQ0512887.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9632..11227)
                     /locus_tag="ACG912_001563"
     CDS             complement(9632..11227)
                     /locus_tag="ACG912_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0512888.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="ACG912_001564"
     CDS             complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="ACG912_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFQ0512889.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14780..15340)
                     /gene="pth"
                     /locus_tag="ACG912_001565"
     CDS             complement(14780..15340)
                     /gene="pth"
                     /locus_tag="ACG912_001565"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFQ0512890.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15693..16667
                     /locus_tag="ACG912_001566"
     CDS             15693..16667
                     /locus_tag="ACG912_001566"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFQ0512891.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16837..18237
                     /locus_tag="ACG912_001567"
     CDS             16837..18237
                     /locus_tag="ACG912_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFQ0512892.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18315..19037)
                     /locus_tag="ACG912_001568"
     CDS             complement(18315..19037)
                     /locus_tag="ACG912_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0512893.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19116..19622)
                     /locus_tag="ACG912_001569"
     CDS             complement(19116..19622)
                     /locus_tag="ACG912_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0512894.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19745..21544
                     /locus_tag="ACG912_001570"
     CDS             19745..21544
                     /locus_tag="ACG912_001570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFQ0512895.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21612..>22123)
                     /locus_tag="ACG912_001571"
     CDS             complement(21612..>22123)
                     /locus_tag="ACG912_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFQ0512896.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT         6126 a         4837 c         3999 g         7161 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataacttttt tgttatgttt
       61 ttgaataaat attttcttct gtcttttgga caagtaataa tataccaact aaatttagct
      121 tggtcaacga attttttgta ttttttcaag tttttttcaa taaacgatta ttcataactt
      181 ttctttcttt atcgttcgtt ttcgtcttct ttaatgtcta gaatagtcgg cattagcttt
      241 tgaactttcg ttattattta ttcctttcta cacagcaaaa gctgcctaag gaattcctta
      301 gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga attgattgtg
      361 catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca gttggcggca
      421 taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg tcatttccta
      481 gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga tcgttcagtt
      541 ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga tctgtgaatc
      601 gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt ccgtatacaa
      661 acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg ataacatgtc
      721 cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct aatgcttctt
      781 ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca tctaccatat
      841 ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg atttttgctg
      901 ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc attacatcat
      961 tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta tgtgtagtat
     1021 caattccttc attacggaaa acgcggccga tttcataaac tttttccatt ccgccaacaa
     1081 ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg tccagtgcat
     1141 tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc aatactggtg
     1201 tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg ataatttggc
     1261 ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag tagcgttgtc
     1321 tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga cgaagtgctt
     1381 ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct gttttcatga
     1441 tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact tcataggcct
     1501 catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga tcttgtagat
     1561 gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct acactagcgg
     1621 tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca tgcaattctt
     1681 ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct tcacgcaatt
     1741 gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact tgatgttgtt
     1801 gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat aatgccaatg
     1861 aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg aaaaacttag
     1921 aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt gatccagcag
     1981 gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt tcgctgctcg
     2041 aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag ctactttttc
     2101 tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt tttcggctgg
     2161 tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt gaatcatcca
     2221 tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat agtcaagcat
     2281 ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa tcgtcaaatg
     2341 ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt
     2401 acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac
     2461 agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac
     2521 tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc
     2581 tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat ctattatatc
     2641 ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc
     2701 aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt
     2761 tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc
     2821 aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac
     2881 gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc
     2941 taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca
     3001 gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc
     3061 acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac
     3121 aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc
     3181 tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc
     3241 gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc
     3301 cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg
     3361 ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt
     3421 ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt
     3481 tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg
     3541 atttttgatg taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc
     3601 tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag
     3661 tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg
     3721 acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc
     3781 gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt
     3841 tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct
     3901 tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata
     3961 aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg
     4021 acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc
     4081 tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg
     4141 tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa
     4201 agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc
     4261 gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc
     4321 agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc
     4381 ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc
     4441 ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg
     4501 acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg
     4561 ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg
     4621 tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc
     4681 ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt
     4741 gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata
     4801 agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat
     4861 cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc
     4921 agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt
     4981 ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac
     5041 tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa
     5101 acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac
     5161 gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg
     5221 ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat
     5281 ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac
     5341 acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa
     5401 cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg
     5461 tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc
     5521 tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc
     5581 agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt
     5641 catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa
     5701 aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct
     5761 tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct
     5821 caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga
     5881 acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt
     5941 gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag
     6001 ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat
     6061 gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg catttttcat
     6121 catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt
     6181 tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt
     6241 gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca
     6301 taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct
     6361 tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt
     6421 gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata
     6481 tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt
     6541 acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc
     6601 aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca
     6661 cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg
     6721 caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc
     6781 aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat
     6841 cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc
     6901 ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga
     6961 taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt
     7021 ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag
     7081 accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt
     7141 ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct
     7201 ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta
     7261 tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa
     7321 tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa
     7381 aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa
     7441 atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt
     7501 gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct
     7561 gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat
     7621 ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat
     7681 ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt
     7741 gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca
     7801 ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt
     7861 ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt
     7921 tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc
     7981 gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac
     8041 ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat
     8101 caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat
     8161 tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt
     8221 tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac
     8281 aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc
     8341 tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga
     8401 agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt
     8461 ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc
     8521 acggggatct ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg
     8581 ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt
     8641 aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga
     8701 cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa
     8761 atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa
     8821 aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact
     8881 agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac
     8941 tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc
     9001 atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat
     9061 ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa
     9121 aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct
     9181 gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc
     9241 atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa
     9301 tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc
     9361 tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt
     9421 ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt
     9481 caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat
     9541 tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt
     9601 taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca
     9661 ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa
     9721 tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag
     9781 cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca
     9841 gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc
     9901 aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc
     9961 ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag
    10021 tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct
    10081 tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa
    10141 ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga
    10201 gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga
    10261 ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag
    10321 gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac
    10381 ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca
    10441 aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa
    10501 tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga
    10561 acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa
    10621 ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt
    10681 ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac
    10741 gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga
    10801 aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga
    10861 tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga
    10921 caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt
    10981 gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac
    11041 cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa
    11101 aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg
    11161 ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat
    11221 gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac
    11281 aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg
    11341 tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc
    11401 ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat
    11461 atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg
    11521 cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc
    11581 atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc
    11641 gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc
    11701 gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc
    11761 gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc
    11821 acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga
    11881 tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat
    11941 cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc
    12001 tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt
    12061 tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg
    12121 accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc
    12181 ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt
    12241 ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc
    12301 ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc
    12361 aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct
    12421 cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg
    12481 ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct
    12541 aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt
    12601 acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc
    12661 gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa
    12721 gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc
    12781 tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg
    12841 tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc
    12901 agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata
    12961 tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat
    13021 ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg
    13081 aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat
    13141 aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac
    13201 ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc
    13261 tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct
    13321 cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc
    13381 ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat
    13441 caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc
    13501 tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca
    13561 acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata
    13621 ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa
    13681 aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg
    13741 ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc
    13801 gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga
    13861 agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc
    13921 ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa
    13981 aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat
    14041 ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac
    14101 ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga
    14161 atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat
    14221 atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac
    14281 atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg
    14341 ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc
    14401 gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct
    14461 ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg
    14521 aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc
    14581 ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc
    14641 aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc
    14701 ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc
    14761 cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt
    14821 ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat
    14881 tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc
    14941 cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga
    15001 taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc actgccagat
    15061 ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg
    15121 ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt
    15181 ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt
    15241 gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt
    15301 atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc
    15361 taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc
    15421 ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa
    15481 tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa taattgatat
    15541 ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg
    15601 ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg
    15661 aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc
    15721 atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag
    15781 ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag
    15841 gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg
    15901 ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa
    15961 aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg
    16021 tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag
    16081 atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt
    16141 caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg
    16201 atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt
    16261 ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa
    16321 tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta
    16381 tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg
    16441 ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg
    16501 gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag
    16561 taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat
    16621 taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga
    16681 cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa
    16741 ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc
    16801 gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa
    16861 aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt
    16921 tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga
    16981 ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct
    17041 gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag
    17101 ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga
    17161 tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt
    17221 caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg
    17281 aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt
    17341 atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa
    17401 agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa
    17461 gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat
    17521 gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg
    17581 agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat
    17641 gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca
    17701 aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt
    17761 gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt
    17821 gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat
    17881 ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc
    17941 gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa
    18001 gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac
    18061 tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa
    18121 tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt
    18181 gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa
    18241 aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc
    18301 taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct
    18361 tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc
    18421 gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct
    18481 cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc
    18541 agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac
    18601 ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt
    18661 tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta
    18721 cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac
    18781 gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc
    18841 acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga
    18901 ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc
    18961 tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat
    19021 ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta
    19081 gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca
    19141 aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg
    19201 aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct
    19261 gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca
    19321 cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa
    19381 aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact tccaccataa
    19441 ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat
    19501 cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg
    19561 actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc
    19621 ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata
    19681 gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt
    19741 tttcatgagt tattcattaa attggaatct agattctatt ttttccggag gcagtcattc
    19801 tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg
    19861 cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt
    19921 gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc
    19981 tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc
    20041 tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga
    20101 ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat
    20161 tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc
    20221 tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat
    20281 tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat
    20341 gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg
    20401 gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc
    20461 tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt
    20521 gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt
    20581 taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca
    20641 agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc
    20701 agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc
    20761 tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta
    20821 ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt
    20881 taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg
    20941 ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt
    21001 cgcagaactg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat
    21061 caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg
    21121 attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa
    21181 gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta
    21241 ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa
    21301 cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa
    21361 gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac
    21421 ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc
    21481 aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat
    21541 ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg
    21601 attaatcatt cttacatgct gacagcaaag tcaggggcaa accattgaac tgaaccaact
    21661 tttacgcttt cgcctggttg aggagcatgg atatattgtc ctccgcctaa agcaatcgct
    21721 acgtggtaag ttccgccttg tgacccccag aataacaagt ctccagcttt tgcttgagaa
    21781 actgaaattt ttgttccagc tgattcttga ggaactgtcc aaccaccgat gtcacgacca
    21841 gttacttgca tgtaaacata gcgtgtgaac cctgagcagt caaagccact tggatcttta
    21901 ccgccccaaa catatggagt accaatatat ttgtaagctt ctgctacgat tgctgcacca
    21961 ttcacacttc cagaaggagc tggagcaggt gttacaggtt ttggttgttc aggtgttggt
    22021 gttggcgttg gagccggagt actacttcct gtgccgtttc ctgtatcaac actttgatct
    22081 gttgatggtg ttgtaggcgc aggtgttgtt gattcttctg ttg
//
LOCUS       DAZKOW010000045        22119 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000045 DAZKOW010000000
VERSION     DAZKOW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.045"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..631)
                     /locus_tag="ACG912_001572"
     CDS             complement(107..631)
                     /locus_tag="ACG912_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFQ0512897.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="ACG912_001573"
     CDS             complement(624..1097)
                     /gene="tsaE"
                     /locus_tag="ACG912_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFQ0512898.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1220..2200)
                     /gene="pta"
                     /locus_tag="ACG912_001574"
     CDS             complement(1220..2200)
                     /gene="pta"
                     /locus_tag="ACG912_001574"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFQ0512899.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2353..3033)
                     /locus_tag="ACG912_001575"
     CDS             complement(2353..3033)
                     /locus_tag="ACG912_001575"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0512900.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3159..4028
                     /locus_tag="ACG912_001576"
     CDS             3159..4028
                     /locus_tag="ACG912_001576"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0512901.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4054..4569
                     /locus_tag="ACG912_001577"
     CDS             4054..4569
                     /locus_tag="ACG912_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFQ0512902.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4616..5179)
                     /locus_tag="ACG912_001578"
     CDS             complement(4616..5179)
                     /locus_tag="ACG912_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0512903.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5234..5413)
                     /locus_tag="ACG912_001579"
     CDS             complement(5234..5413)
                     /locus_tag="ACG912_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512904.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5490..5999)
                     /locus_tag="ACG912_001580"
     CDS             complement(5490..5999)
                     /locus_tag="ACG912_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512905.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6095..6475)
                     /locus_tag="ACG912_001581"
     CDS             complement(6095..6475)
                     /locus_tag="ACG912_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0512906.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6805..7554
                     /gene="pnuC"
                     /locus_tag="ACG912_001582"
     CDS             6805..7554
                     /gene="pnuC"
                     /locus_tag="ACG912_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFQ0512907.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7875..9092)
                     /locus_tag="ACG912_001583"
     CDS             complement(7875..9092)
                     /locus_tag="ACG912_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0512908.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFSNRLTPYALTLIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKKALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(9350..10627)
                     /locus_tag="ACG912_001584"
     CDS             complement(9350..10627)
                     /locus_tag="ACG912_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512909.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(10706..11350)
                     /gene="pcp"
                     /locus_tag="ACG912_001585"
     CDS             complement(10706..11350)
                     /gene="pcp"
                     /locus_tag="ACG912_001585"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFQ0512910.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(11549..12493)
                     /locus_tag="ACG912_001586"
     CDS             complement(11549..12493)
                     /locus_tag="ACG912_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFQ0512911.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(12495..13181)
                     /locus_tag="ACG912_001587"
     CDS             complement(12495..13181)
                     /locus_tag="ACG912_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFQ0512912.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13655..13975
                     /locus_tag="ACG912_001588"
     CDS             13655..13975
                     /locus_tag="ACG912_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFQ0512913.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            14129..15034
                     /locus_tag="ACG912_001589"
     CDS             14129..15034
                     /locus_tag="ACG912_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0512914.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            15031..15804
                     /locus_tag="ACG912_001590"
     CDS             15031..15804
                     /locus_tag="ACG912_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFQ0512915.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15985..17409)
                     /locus_tag="ACG912_001591"
     CDS             complement(15985..17409)
                     /locus_tag="ACG912_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFQ0512916.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            17620..18066
                     /gene="nrdI"
                     /locus_tag="ACG912_001592"
     CDS             17620..18066
                     /gene="nrdI"
                     /locus_tag="ACG912_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFQ0512917.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(18131..20113)
                     /locus_tag="ACG912_001593"
     CDS             complement(18131..20113)
                     /locus_tag="ACG912_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFQ0512918.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(20127..21023)
                     /gene="murQ"
                     /locus_tag="ACG912_001594"
     CDS             complement(20127..21023)
                     /gene="murQ"
                     /locus_tag="ACG912_001594"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFQ0512919.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(21026..22087)
                     /locus_tag="ACG912_001595"
     CDS             complement(21026..22087)
                     /locus_tag="ACG912_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFQ0512920.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
BASE COUNT         6363 a         4651 c         3980 g         7125 t
ORIGIN      
        1 tttattcaaa aaaaatggac tgcgacacga cttttgtcac agtcccttcg tgtgtttttc
       61 tgccttctca aaaggatgac agtttctcac tcttttccta tttcttctat tcaatcaaat
      121 aactcatttt atatacatct aaaaattcac ctagatctgt tcttgcccct ctaggcatga
      181 ccgcttcgat ttggaagccc atttttcggt aaaggtgtac ggctctttca ttacgaactt
      241 gaacgtcaag ctccaatcga aaaagtacac ccatttcctt cgcccaactg atgatttctt
      301 ctagcataag agtgcctagt cccattcccc aatattcctg cagaatactg ataccgactt
      361 ctcccacgtg agacaaacga aattgactgt ctgccttgac agatgctgtt ccaataatcg
      421 tcccttcata gattgcaagc agcaataaat tattattgct ttcgtatata taatccaact
      481 cttcttctaa ggtttctggt ggtaacagga gttctgcttc atccaaaact aaaaattctg
      541 tctccttatt aacttgtccc atcatcaaaa gaatgtcttt tgcatctcct ggcactgctt
      601 cccgcaagat gatcccaact tcttcactca tgattttcag cccatttttc tagaattcgc
      661 tgtttgaagg tttcactttg ctccccgtat gagattagtt tcacataccg cttttctaaa
      721 tctgtatcac ttttttctaa aatcagttcc aaataatcct ccggcagtgc ttcttctaat
      781 agatttcccc attcaacaac ggaaagccca tcaccttcaa aatattcatc taaccctaaa
      841 tctgctccgc tagctgctac ccgataaata tccatatgat acaacggaat ccgaccttga
      901 ctatattctc taataatcgt gtatgtcgga cttttgatca tttgcgttat ccctaatcct
      961 tgggcaattc ctttcgtcaa cgttgtttta cccgcaccta gatcaccagt caatatcaga
     1021 ttgtcacctg gcattgcgga atgaccaatt gcctctgcga attgttctgt cattgttaat
     1081 cctgatagtt cgatcatttt tcatcttctc ccgctcttct ttctttttgc cagtatatcg
     1141 cgtaatgaag tttttttaaa gaaaaaatga actagtcaag aacagcagct gctgtttttg
     1201 gctagttcgc gtgtatttgt taatcaatca atgtttgtgc tgctgtgata atcgataatt
     1261 tatatacatc ttcttcattt gctccacgag acaaatcaga aaccggtttg ttcaagcctt
     1321 gtagaattgg tccgattgct tcaaagttac cgaaacgctg agcaatttta tagccgatat
     1381 ttcctgactg caattctgga aatacaaaga ctgttgcttg accagctact tctgaatctg
     1441 gcgctttcag ttgtgcaact gaaggaacat aagaagcatc aaattgcaat tcgccgtcga
     1501 tcatgtattg tggcgctagt tttttagcga ttttagttgc ttcaactact ttgtctactt
     1561 ctggcgattt cgctgagcct tttgtcgaga agctcatcat tgctactttt ggttcgatat
     1621 cgaacaattc tgctgttttt gcagaatcta cggcgatttc agccaattct tgcgcagttg
     1681 gattcacgtt gatcgcacaa tccgaaaaga catatttttc ttgatctctt ccacgtacca
     1741 tgatcatcgc tccgcttgtc cgactgatgc caggctttgt tttgatgatc tgaagtgctg
     1801 ggcgaacagt gtcgccagtc gaatggatcg ctccagagac cattccgtct gcaatcccca
     1861 tatacgtaag catcgtacca aagtaattca catcttttaa aatcttttct gcttgttctt
     1921 tggatgcctt tccatttcta cgttcaacaa aagtatccac catttcatcc cacttatcgt
     1981 aattatcagg gctgatgatc gtaaagctag atgttttgat tcctctggca tgcgctgcat
     2041 ttcttatttc ttcttcgttc cctactaaaa tgggttccac aagttcttct gctttcaacc
     2101 gggcagctgc gcctaaaata cgagcatcag tagcttccgg gaaaacgatt ttgatcccac
     2161 ggcgaacaat tttaaatctt aaactatcaa acaattccac aagatgacct ccagacgttt
     2221 ttatatgtat tatcatacac tattccagaa aaaaagaaca gtttatttta tctgtatcag
     2281 tcaaaaatgc tggaaaaatt aagtttttca catacaaagc gaactgcact ataacaggaa
     2341 agcgctgttt tattacactg tttcaggcag ttgccaatca atcggttctt ctcctaacgc
     2401 aaggagcgca tcattcgttt tcgagaaggg tttggaacca aaaaagccac gacttgcaga
     2461 taacggactg ggatgtggag atgttatgat gatatggcga ctcttatcga tcattttgat
     2521 tttttcttgt gccggttttc cccacaagat aaacacgatt ggtttttccc gttcatttaa
     2581 tttttcaatg atcgtgtctg ttaatctttc ccaacctttc ccgcgatgag aatacgcttg
     2641 cccttcccga acggtaagaa cggtgttgag taaaaggact ccctgtttag cccatgacac
     2701 tagatttcca tgttgaacag gtgcgattcc aagatcatca taaagctctt tatagatatt
     2761 tcttaacgat ggcgggatct ttacgccagg ttgcacagaa aaagataagc catgagcttg
     2821 attttctcca tgataaggat cttgtcctaa aatcaccact ttaacttctt cataaggagt
     2881 caattccaat gcttcataga tatgatacat atctggatgg atcttttggg acgcatattc
     2941 tttttttaaa aattctctta gatgcagata ataatctttt gaaaattctg aggacaaaac
     3001 ttcttgccaa ctgttatgaa tgatttcttt caaaaactcc acctcctaat aaaaagaata
     3061 actcacattt tcttcaatca caagggacaa tcttcaccct cagcacttga ttaatatggt
     3121 aaactaggaa caagaagcag aaaggaacga taatcattat gatcaaatta attgcatcag
     3181 atatggacgg cacattatta gatgcccata tgagtgtttc tcctgaaaat gccgaggcga
     3241 tacgttttgc aaatgatgcc ggtgttgagt tcatggtcgc tactggtcga aactatcaag
     3301 aagctcgcgc agctttagat gaggtaggaa tcgattgtgc gatgatcact ttgaacgggg
     3361 cacaggtatt tgataaagaa ggaaattcat tgtttaccgt gtcgattcca aataatcaag
     3421 caatctctgt tttagatatt ctagatgcta acggtattta ctatgaagta gcaaccaacg
     3481 atggtcttta ttctgaaaat caagcgaaac gaattgaaag ttttgcttcc atggtggcta
     3541 cccatttacc tcaccttacg tataaaatgg cgattgccat ggcctcagct aagctggagt
     3601 tattgcacat cacatacgtg gacagtattc gtgctatttt ggaagaaaag aatctggaag
     3661 ttttaaaaat tatttgtttt catacagaag ggccagctgt attaggtcca gttggaaagc
     3721 agatttctct tttaggcgat ctagccgtta cttcctctgg tcaaaacaat atcgaagtga
     3781 accataaaaa cgctcaaaaa ggaattgctg ttgcccatgt cgctcacgag cgtgggattt
     3841 ctttagaaga agtcatgacg attggcgata acttcaatga tgtcagcatg ctgcaagtag
     3901 caggagtcag tttcgcaatg ggaaatgcag aaatcgaagt aaaagattat gccaaatata
     3961 caacggacac aaaccttgaa tctggcgtag gaaaagccat attgcgcgcg attgcagaag
     4021 acttgtagtt ttatctagaa aagagggata tagatgtcag aatttttcat tcaggaaaag
     4081 cagcttggac gtgcaactcg gacaattgtc aaaaacgaaa aaggacattc cctgtttttg
     4141 atggttggtc gctggggaac aaagggcgat gcattgtctc tttatgccat gaatggcgat
     4201 ctcgttgctc acatcaagca gataagcctg acattcggta cgcgttttga gctgtataaa
     4261 gagttcgaaa aggtgggaac catgcagaaa atattcaatt ggcccggtga cttttattat
     4321 attcgtagac ttcactggac agtccaaggt gatatctata atcatcaata tcaaatccat
     4381 catttcaatc agcaaatcat gaaaatggat aaagcaacac ttttttcagg agattactat
     4441 gtattggatg tctcaaatga tgaagatgct ccagtttgta tttgtattgc tgcggtttta
     4501 gattactggc tctaccgtaa agacaaaagt cgcgataagc ctgtttctta ccgacttaac
     4561 ttaagttaaa aagatgtacc tgaaatgcgg aaatacgcat ttcaggtaca tctttttaga
     4621 tattcttttg catatgatca taaagatgac cactgatcat ccgctgaccg acaactttga
     4681 agtcatggcg ttcgtataat tttttagcct ttggattttt ttcatccaca cttagaccga
     4741 tgacggagcg attagctttc ttcgccaatt taggtaatgc ttctaataat cgcgaaccga
     4801 tgccttgtcc tctaaaatct tctgatacac tgatagagtc caaataccat tcgttgggaa
     4861 acgtttcttt atcaatgaac aaacgtgtag acgcatctag ctgatatttc tctaagatag
     4921 gtaccagtgg ctgatcgatg attgattctt cttcatctgt ataaccgtaa gcaactcctg
     4981 caactttacc gtctatttct tctacgatac ctcgtgaata actgtaacga taatttggat
     5041 cagtgattgc ttcttctaaa atctctaaag tcttttgttc ccctgtctgc ttcaaaaatg
     5101 gcagttccat atcttttaga atcactaaga tcaatggggc gatttctgga gcatcttctt
     5161 ttctcgcaaa acgaatcata gtttcctctc cttttctcca ttctaacatg cagaaaaaga
     5221 aatcgcaaaa tcgttatttt aaaaaatctt caatcttttc agtaatgaaa taaccatgag
     5281 taacgattct tgccgcttca gttcggcaat aagggcagac gactggcatt tctttatcat
     5341 tatgttcgac tacattattg acttcttcgt atgcaaattt ttttccgcaa ttagggcatt
     5401 tcattttttc catgttatct caacttcctt tcatctttac tctcttagct tactcttata
     5461 ttgtaaaaaa gagaagcaat atccttcttc tatgcagata tccatacctg acggtcctgt
     5521 tctccatcta ttaatcggac tttataataa ggacgttcat ctttgactag aagtgtccac
     5581 tctttcactt ttgctttttt cccgtagtag ctgactgctt ccttcatcgc tgcttgcggt
     5641 tgtttgaact cttccgtttt ttctatatca aaaagatgtt ctgcggcctt ttccgtgtca
     5701 tttcgtacgg ttgttttccc agtggatgga ttgactgcta tgacttgtcc ttcacaaaaa
     5761 aactggtatt catatgttgc attcgtagga ttctccaaat ctttttcaac tgcaaaatga
     5821 atagcagata tgttatgttc ttttgtttcg ttttttacga ttgctgctgc ttttttcaat
     5881 gagatccgat attcctggtc actaatatca tccatgtgaa aagcggcatt gagcagtccc
     5941 gtcataccgg taatccctat tacgatgagg gcagcaacac caaacatcat ttttttcatt
     6001 cttcattctc ctgtttcttt tttttaaaga atatcaagaa atgaacgaat gaaaaatcga
     6061 atgaaacgaa aaaaaagtga caattttgtt atctttattc gtgcagttct aatggcaatc
     6121 cgtctggatc aaagaaaaac gtcatttttt ttcctgtaaa ggtatcttct ctaatcggtt
     6181 ctgattcgat ccctaaggac tgcaattctg caattgtttc ttcaatatcg tctactttga
     6241 aagcaagatg acgcaagcca caagcttctg gatagtttgg tcgttgtggc gcattagcaa
     6301 ttccaaaaat ctccaattca gcacttccta gtttcagatc taatttatag tcatctcgtt
     6361 ctttgcggta attttctctg attacttcaa atcctaattt attaacataa aaatcacgtg
     6421 atttctgata atctgaaaca atgatggcta tatgatggat cgttgtaagg ttcatatgag
     6481 cactcctatt cttcgtttaa gctcggatct atattaatct ccagttccat tgtaaatgtg
     6541 tcagattgaa aaaaatagct gtttttcctt tttataagaa aaaaggcaaa aaaaacagaa
     6601 agtaggtcct ccaagacact cttcctgttc ccaatataat aaatgaaagt aagaaattat
     6661 attttcaaga aaacaaacag aaaattatca taacacaaat tatttataat tgaaagacta
     6721 gttttttttc aagaaaagca tacaatagat tgtgttagta aatacagaac acactacatt
     6781 tagtgtctta aggagtcctc atacatgaag tcatattttc agtttttagc tcatcagcta
     6841 aaaggctggc cgcaacaaaa ttattatctt ttctttttca gtcttggctg ccaggtcatg
     6901 actcttgtca atagtcccat cacttggctg tccgttatta catttatcgg taccacgctt
     6961 ggtgtattgt gtattttatc catcaatgcg gcaaaatcag tcaacggtat tttaggtatt
     7021 ttgtctgcga tttgttttat tatcgttggg ttttcagcaa agaactattt aagtatcggt
     7081 gaacagattg cttatatgat cacattggat atccccgtat tgttaagtaa agaatggaat
     7141 cataatatgg cttcaaaaat ccgaaaattc actggaaaaa gctggtttat cgcaatcatc
     7201 agtactttcc ttgtctatct tgtttcagga ttcctgattg gtcgttatac agatgatcct
     7261 cggccgtggg tagatgccat tgctttttct attagcttga ctgctggtat catcagtttc
     7321 ttgcgttaca acaaccaata ttactggtgg ttagcttcag ggcttgctca aatggtcctt
     7381 tggtatattt catttacgca tggatctgca agtttggcga tgttcgtgaa cagctctgtt
     7441 tacttgctga atgatgtcct tgcatttacc gtttcacctt ggtataacca aaaagaacgt
     7501 gaacgtatgc aaaaaatgga agaagaatac tacaacgcac aacaacacgc ataaaaaaac
     7561 tgagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     7621 ggaatcagaa gcaactcctt cggaaataag ctgaaattca caaaaatttg aaaagcaatt
     7681 ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct ctatttccta
     7741 cgattagtca agtaaaaggt gcaagaaaaa tagctcgtaa acgaaagcga caaaatcaaa
     7801 gcacaccaaa taaagccccg ttcgtaaaaa attttcgatc tgggcaaaga agtggtatac
     7861 tgtaagtggt ttagttaatg gtccactgag accgcgtacg catagtcgta ctctgtatgt
     7921 gccctaacca tagcgtgcat acatttcaga agcttgttca tacaagcaac tgtggcaacc
     7981 ttatctttct tagggatagg ttgctttttt aatttgtagt aataatcgac aatatgatta
     8041 ggagctgcgc gttgctggcg aatcatattt cgaatggtga agaaaataat tttccgtgct
     8101 ttgggattac cacgcttatt gatgcggtct tggccagtat acttacctga atgataccgt
     8161 cggatgtcga ttccgacaaa ggcattcaat tgtttatggg ttttaaatcg tgtgatatct
     8221 cctaattcac cgagcaataa agcggctgtg agtgcgccaa tcccagggaa cgtttggtac
     8281 aacaaaaagc aaggagagcc ttcggctttt ttgatcattt gtgtcgccag tgcttttttt
     8341 tgttcgagta aatcttggag aagttcggcg taatagatcg ttttttgaca gtggatactg
     8401 tctttttcaa cagctggata ggcacactgt gcatagtgga tgatttgatc tgccttttgc
     8461 tcggcacgat tcgcggagat ttttttccgt gtttcgttga tcagtttatt ttttattttt
     8521 gtccgagtag atgccaatac aaaatctgga tggggaaaaa gacggatcaa tgtcaatgcg
     8581 taaggggtca gcctgtttga aaagaactgt tcaagctctg gaaaagtcaa ttgaaggcag
     8641 ttgtgtaaat ccatgcgcag acgagtgatt ttcttctcaa tttcttgata aaaacgggct
     8701 aaatcgcgca tctctaagta aaaattgctt tgtacttctt ttggctgacg ttgattttta
     8761 agatgagtct gtgcgagccg gtgggcatcc gatttatctg ttttccaact gcgtaaggtg
     8821 ctttcttcca tctgtttttt cgcctctaac gggtttaaca aacagtaaga aaagtgatga
     8881 tcttgaaaga acttttcgac tggtctggaa taaatccccg ttgcttcaaa aacaatcaat
     8941 ggacgctcag gcaagtgctg gatctcttcg agtaatgcat gaaatcctct ttgagtgtgt
     9001 tcaatctcgt cttcaaataa gcaggtgtcg tctttgtata ggacactgta gctgtgtccc
     9061 attgaaacat ctaatgcaag aatgtaaggc atttgttttc ctccttttta agttttgaaa
     9121 gaactttact gcttcatttc gattccaatt tcctatacac gggctcatgg ccccaacata
     9181 cgtaaacctg attctaaaat gacagtaaag aagttcggtt tttgataggg actcgtagtc
     9241 ctcaaggctt tgttcgaact ttctttcact cttcactata atctaaaaat agaaaataga
     9301 ggagaacatc attcgtcaat gatgttcccc tctaatctta gtatgttttt tatagtaatt
     9361 ccttcagata ctttcttgcc tcatcattga aacgcagtga ttcttctaac ttctgataat
     9421 attccaacgc ttgtttctga gcttcagtaa aacgataatg ttttcctttc catttgtcga
     9481 ttgtctcttg cacatttcca gattggatat ctttcactac tagttgccat ttttcttttg
     9541 agactgtacg gtctttctta tcagcagatt gtatagcttc tcgttcttta cgcaatactt
     9601 ttaaaaaacc atttctgact atccgaggaa tgctgtcgaa gaacccttct gtaaaaccat
     9661 agcgacaata ttgcttccaa ataaatagat agatttttgg ttggatgaat cgttggtttt
     9721 tcgcaaaata ttccatctga gcagtgactt ctggtgttcg ttgacgtttt gtctttctca
     9781 acacggaaat aaatgtttca aaataagcat taggatgtac tggcacttct tcatatacga
     9841 tggattgcca atctttacgt aaaaatgcgc gatagttgat caagaattct tctacatcct
     9901 ttactaattc ttctgcaaag tcgtcaaata gttctttttt tatctgtcgc ttttttgact
     9961 gcccattgat gtgttcactt attttcttga tttcattttc caatgcttga taatccgcta
    10021 tgtaaatcgg catttctact tggcgaaatt ctgcgagccg ctgagctaat tttatatccg
    10081 gtaaatatgg ctgtttcatc cgacgatttt ggtacaagac aaaacagtat ttttgccata
    10141 gtccttcagg aaaatcgata ttcttctgct taagccataa taattcgtct aaggccagat
    10201 caacttttgt cattccctga tggatcgaag ctgccactgt cggagagaca tgcaggtgat
    10261 cggcaaagtg gttgatgcct agttttagga tctttccggt ttttggcgat ttaacgatgt
    10321 attgcacttt taattctcta ccacactcac aatacaattt ttgctggctt cgttttgttc
    10381 gaaaaaacgg attgatcttg aagtcagaaa acacccaatc tgttgcagct aaataatgtt
    10441 cttccataaa acgagagctg attagatatc ggcggtgctt atcgactagt tcccgctgtt
    10501 ttttcgttaa ttttgcatag atctcccaac gttgactgct tgtgtattgt ttagaaattt
    10561 tgatgggata gcttttgctg atttcttttg gaaaccattc ttcaaagacg gattgccatt
    10621 cttccacggc tgtttctttc ctttctaatt acaaaaagct ggctactaac ataacgttag
    10681 caaccagcca gtgtttcttg ctaaattagt gagttgttcc ggcatttccg gaaatatctt
    10741 ctttattttc tactgctgct tcaactgcat aagtcaaaga agaagcaatg tcttctaaag
    10801 acatagaagg cgtacccact ggacgatcga tcacttgatc gggtgagaat ggcacgtgga
    10861 tgaacccacc tcgaattgcc gggaattgtg tttcgatcaa atgaagcaaa cgatacatga
    10921 tatcattgca gacaaatgtt cctgctgtat aagagatgta cgctggtaga ccatgatctt
    10981 gtacattttt tgtcattgct ttgattggca atgaggtaaa gtaagcggct ggaccgtttt
    11041 cttctaaagc cgtatcgaat ggctgatttc cttcattatc agggattctt gcttctgcta
    11101 gattgattgc gacacgttcg aacgagactg ctgaccgccc acctgcttgt ccgacacaga
    11161 tcacgacatc tggttggtgc tgttggatgg cttctttgac tacttgactg cttttttcga
    11221 atacggtcgg tacttccact ttgatgattt cagcaccgct gatttcatct ggcatttttt
    11281 ttactgcttc ataggctgga ttcactttgt ccccaccaaa tggatcaaaa cctgttacta
    11341 gtactttcat ctgttgttcc tccgtccgat tgatttaaca tatggttatc ttttatcata
    11401 ccattttcct tgaacagcgt aaataaagga aacaatagtt taaataacat aacagatcaa
    11461 acttaatata ttcttataaa aaaatagtgt tccactacaa tgttagaaga ggttgcgaca
    11521 attattctgt cacaacccct ctcttctatt atttaaatag aatcatataa gcaatttgga
    11581 aaactagcat aaataacgca ataaacaact ggtttttgat cactccgtat ttgtctttca
    11641 tctccagcat cgctacaggc acgatattga agttagcagc catcggtgtc agcagggtcc
    11701 cacaaaaacc gcaagtcaat gcaaccattc ctattaacgc tggattcgct ccatgactaa
    11761 atacgaatgg tgcaccgatc ccaaccgtca tcacggtgat ggccgcaaat gcattcccca
    11821 tgattaccgt aaatatcacc atgccaagag cgtaaatgat gatgccgatg ttcacatttc
    11881 cttctggaac gatcgtacct actgcattgg aaatcactgt cccaactcca gcgcttgtaa
    11941 ataccgcacc tagagaagct aaaagcattg gcaacatgct taacggccct actgtgccca
    12001 gcatatctgc tgcatcatct aaaaatgtac ttggtcgatt atctcgagaa aagaacatca
    12061 agattagcat ggctgcaaaa acaccgacgc ccacgccgac taaagcacct aaattggtaa
    12121 atagagcaaa caagatcgca aagacaccga tactcaaagc tggaataaaa attttcattc
    12181 ctagtttgtc tgacattttt tccatatatt cttttgatgg aagccggctt tctcctttgc
    12241 tgactttctt agcaattgct ggtatggtca tcgcaaagat caagccaccg ttcacgattg
    12301 ccggaagcca tcttcctcct gcaataacta cccctaagac cgtccagaaa aaggctgacc
    12361 cataacgatg aggattggtt ttatctgtta aatttcttac tccagtgtaa atcaatacga
    12421 gtcccatcac gatataaagg atctccagta atttatcgcc taataaaata tcaggatttg
    12481 taaaaaagtc catcttattg cccctttcct ggataatatt tcttcgctaa tttcttttct
    12541 ttaagataga agtagacact tgccacaact aaagccgtga tagccactgg aacttccact
    12601 aaagcaagct gtcccaatgt tacgtcatat cctagatctt tcagtgttga ttggactagc
    12661 aacgcccctg ctcctccgat gaatagtact tggccaaaga accagcagac attttccata
    12721 gctgaagcca taccttttag ttcttcttca tgcttaggat ggacttctaa cccttttgat
    12781 tcgatggtcc ccaatgccat cggtaaaatg attggacgaa caaatccagc aactccacca
    12841 aaactcacgt taaaagccgc aaagatcatt cgaaagatcc cataaatatc tacaatcata
    12901 ccagcagata ctttcttgaa tcgcttgatc aaggaagccg ctgattcttt cagaccattt
    12961 ctttcaagcg ttcctgttgc caacataatg atgataaaaa tcgccatccc gcgattgttt
    13021 acaaaacttg tacctaaaag ttctaacatt ccttgaatgc caagaccgcc agtcaaagct
    13081 gtaacaacta aagcaaccat tacgatcaat atcgagtcta atttaaatgc aaacccgaga
    13141 ataacaatca agacgcctaa aaggctaaga taattttcca tatattttcc tccataaaat
    13201 gacaatattt ttttaattat aaagcaaaac acataaaaaa tatagttgtt ttctcacgaa
    13261 atagacacta tttttaaata tttttcgtta tttttttatc acaaaaatta tcttacttat
    13321 tgtaaggtaa agcttttttt aatttattgc ccgataatat atagaaataa ggtctagtta
    13381 ctgttttttt taaatgatta ataaataaca acttctttct atgaaattgc aaagcattca
    13441 tcttcttttc cgctaacgat atatccgtca gtgcttggta cactttacta aaaagtatga
    13501 gattgccgat atctggatag gtgtgctata ctacagaaaa agatatacaa tctgccaccg
    13561 ggtaggataa agaggatgat cattccatta ggtctttgaa gggatgattt tcctcttttt
    13621 gtgtattttt ttatagttaa ggaggaatgt aagtatggca tggatggaat taatcatagc
    13681 aggaattttg gaagtattct ggtcaacgat gatgaaatgg agtgatggtt tttctaaaat
    13741 caattattct ttttacacag tgattggcat gattgctagt ttttactttt tgtcaaaggc
    13801 aatcaaaagt ttgccgatga gtctctctta tcctatctgg acagggattg gcgcagttgg
    13861 ttcggtcata attggtgtgg tcttgtttca tgataagctg aatctttcca cttggttctt
    13921 tgtggggatg ctactcatca gtatcattgg catcaagatt actagcggcc actagacact
    13981 tggaataagt actgatcaga aaaaataagc ctttttattt aatattcaat ttttttatga
    14041 aaaaataaga aataagtcca atgacctagc gacttctcca ctcatcaggt gtatgcttgt
    14101 actatctata gaaaaggagt gaatattttt gaaaagtctt tcggtccaag aattatccaa
    14161 gaactttggt cacaaacaag ccctcagcga tgtcagtttt agcttatccc ctggcaaaat
    14221 cgttggatta gtaggtccta atggcgctgg aaaaacaacg atcatgaaag ctattttagg
    14281 actgatccat tactcagatg gaacaatctg tattggtgaa aatgagattt ctccctctag
    14341 tcataaaggg ttggaacaag taggagcatt gatcgaatac ccgggtatct atccattctt
    14401 aacaggctat gatcatctga agttattctc tgaaaccaac gatgtatctg ctatcgacac
    14461 tatcgtgaat cagctcaaga tggaaaagta cattcacaaa aaagcgaaat cctactcgtt
    14521 gggaatgaaa caaaaattgg gaattgctct tgccttactg aaccatccgt ctatcgttat
    14581 tttagatgag ccgatgaacg gtttggatcc acaagcaaca agagatgtgc gaaaaatcat
    14641 taccgacttg gctactcagg gcacctcttt cttgatctcc agtcacttac tcagcgagct
    14701 tgaaaaaatg gtcgatgagc tgatcctgat caatcaagga aagatcatca aacaatgtac
    14761 aatggctgaa ttgaaccaaa cagcgcaaga tttcatcgtt atctctacaa caaatgatct
    14821 agctgctcga acgatcttga cagaagctgg atatactttg gaagatcctg ataaaatcaa
    14881 gcttttaaaa gaaaacgaag aattattagg aaccgtgatc cagctattgt ctgcacaaaa
    14941 tatccatgtg acagacgtcc agcatctgac gaatgatttg gaatcttctc tattggaaat
    15001 tctggattca gtaaaggggg aagaagaaaa atgacattgc ttgtaaaaca agaattattt
    15061 aaattgatca agaaaaagtc aactgctgtt ttatctgttc tattagttgt tttgctcata
    15121 ggaacagcac ttctagccaa aaaatacacg acgatcattg atccagttga aatgactgct
    15181 cagcttttta gtgccacatc ttggattgtc tttatcatga ttgcagctgc aagcacgatc
    15241 atttcgatgg aagcacaata tggtacattg aaaaatctcc tttatcgaaa gtattctcga
    15301 ggagaaatat tagtaagcaa atggatcaca ttagttattt attctgttta tttatacctt
    15361 ttagctatta ttgtcactgt gttgatgaaa ctgattcttt tcccttcgat ttccttcacg
    15421 gagaaggttt ctactggtca aacactgatc caaagcttgt tcacttatac tttgggtagt
    15481 tatatcggtt tatggctgat tttaagttta gtgttgatga ttgcttgctt tatcaatagt
    15541 tccggcgcat ccatctcagc aggaatcgtc ttttattttg cttcgtcaat catatctggt
    15601 atccttattg cactcatcca aaaatgggag tggatcaaat ggaatccgat cagtatgttg
    15661 aatctgcaaa accaaattgg aaacgaagag atcatgaagc aattgactca tctttctact
    15721 aatcaaatgc tattcggtaa tttagcttac atcgttttgt tcttagcatt aggatatctc
    15781 gtatttaagc gaaaaaatgt atgatgactc atcaaaaaag aggctgtgaa agagacgttc
    15841 agccttattg ccgattggct gtctcttaaa caccgtttat tctgaaaaag gagctgtgac
    15901 aacatttgtg tcacagctcc tttcctagta tgtttttgaa taaacgacag gctcacaacc
    15961 tctctttata cttcttttat acttctatac gtgaagcatc ccagtggcaa ataaaaccat
    16021 tgcaatcact ccgaccatcg tcacacctat aagtaaaatc ttatctaagt ggatatgttg
    16081 tacaggctcg ttttgctcct tacgtgcttt gaggtaaaaa taaatgcctg gcaagaaaag
    16141 aacaagaaca agcattactt cattaaaggc tacgactagc atagcccata cttgaaaggc
    16201 acttgctaaa agccctacta aaaattggac ccattctttc cgctgataag aaagtttcat
    16261 ttggtacaat cctaagaaaa gccacgtgaa gaaaatagct gctgtaccaa gtgtgtaagc
    16321 aaagttataa gcactgtcag tgaataataa tgaaagtaaa aagacattcg tacataacgt
    16381 cgtcaatacg atagcataag ttggtgcccc atttttgttg agttttcccc atgctttagg
    16441 caaaagtccg tcttcagcca tgtttcccat tgtttcaatc ggtaagatcg tccatgagag
    16501 ccaaacacca actaaagaaa tgatcaaacc gccgttgatc acgactgctc cccatttacc
    16561 aaccatcatt tcaaacacat agcccatagc aggctgcgcc ttgatcgttt gcagctgatt
    16621 catcgtcatt gtcccatatg gaagcatgga aaccaagacg taaataataa tcaagccgaa
    16681 tatccctaga atcgttgctt tcccaacatc tgattgtttt tttgctcgtg aacctacgac
    16741 agaggctcct tcaataccaa caaataccca aagcatcacc atgatgcaac tcaatacttg
    16801 actggaaaac gggatcggct gtccattcgc attcaatata atgtcgctag aaaagttatc
    16861 aatgaattgt tggatcgaaa acgtgctgat agctactaca ataaataaaa ataatggcac
    16921 taatttacag aatgtgacga tagcattgac gaaactagca ttttcgactc cccgattcac
    16981 tagccatgca tatccccata aaaagactgt actcatcacg atcgatgcca gattttgccc
    17041 accttcaaat actgggaaga aatatcctaa tgcgctcatc aacaatgttg caaaagctac
    17101 gttgcctaac cagcatgtga gccagtaacc ccaaccgctg ataaaaccag cgaaacggcc
    17161 aaaaccttct tctgcgtaac taaagatccc gccttttaaa tctggacgtt ttttattgat
    17221 attattgatc gacaaggaga gcatcaacat tgctgttcct acgagtaccc aactgaaaat
    17281 agctgggccg ccagccgatc cggagatgtc cgtcataata ccaaatacgc cacctccaat
    17341 cgctgaacca attactaagg cagcaagtgg aattaaacca attccttttt tctgttgctc
    17401 tgtatccatg taagcatcct ccaaaataaa accgctttta tcaaaaagta taacaaacct
    17461 tttttatttt tacttaatta ataaaaactt taatagaaat ataattctac cggctttttt
    17521 ctcattatac aagcaaagcg cctagtccct ttccaaatta caccaccaaa aaacttaaaa
    17581 aagatgctat actaaaagga aagaattgga ggatgatcca tgaacatttt atatatatcg
    17641 atttcaggaa atacgcgtgc ctttgccaaa catctagcag aatatgctga aaaaatgcat
    17701 gcagaagatc ctgtcaatcc tgaagttact ttaaaagaaa tccatgaaaa tagtgatttt
    17761 acgaaagaaa cagaaccatt ttttactttc gttcctactt atttagacgg cggaaatggt
    17821 ttagataacg gtgatacaga aatcttgacc gaaacgatgc gtgaatatct ggaacatgaa
    17881 gacaatcata aactttgtct tggtgtagtc ggtagcggaa ataaaaactt caataatcag
    17941 tattgtctaa cagccaaaca atatgcacaa acatttggct ttcctttctt ggcagattat
    18001 gaattacggg gaacaccatc tgatgtgaca cgaatttatc agattttagc agaaaacaat
    18061 aaataataaa aaagaagaag ctgaaaaata gatttcagtt tcttctttcg tttctattgt
    18121 ttctatcata ttaagctttg acatccatca caggtgcttt tgcttttgct ttacctgttt
    18181 tttccaagac aaagtttttt acacgatcca tattggttac caagacgatc atggatgttt
    18241 ctttgcctgc tgctttgatc gcttctaagt cagcttctgc tacaagcgta tcaggtgtca
    18301 cttgctgccc ttctgttact ttcacatcaa atggcgtacc gcctaagtca actgtgttga
    18361 ttcccatatg aagcaagatt tctaaaccat ttggcgttgt gatcccaata gcgtgtttcg
    18421 ttgggaagac agagctgact accccagtaa ctggagaata gatatgtccg ttttctggtt
    18481 ctacaaagta gccttcaccc atcatctttt gtgcaaaaac tggatcactt gctttatcta
    18541 aagaaacaac tgttccgtca gcaactgatg agaggatagt cacactttca gctgaagttt
    18601 gaggtactac tttttcaggc acttcattta ctgtggctgt ttcagtcgct aattcttctt
    18661 tttctaaacg gtctttagga ataccgaaga agaatgtagc aacgaagcca ccagcataag
    18721 ctgccaataa tccaaggatg tagcctaacc atttaccgtc agagatcaat gggatcaaag
    18781 cggctcctga aggtccaata gcgatagccc cgacatttcc gatcattccg atcactgccc
    18841 cgccgatacc tccaccgata caagcagtaa taaatgggcg tcctagaggt aatgttactc
    18901 cgtaaatcaa tggttcgccg attcctagga tcccgactgg taaagctcct ttgatcattt
    18961 ctgttaattc tttatctttt ttgcagcgaa tccataatgc caatgctgca cccacttgtc
    19021 ctgcaccagc catcgcaaga attggaagca atagcgtacg accagtttca tcaatcattt
    19081 gcacatggat tggagtcaaa atttgatgca gtccgaacat aaccatcggc aagaataatg
    19141 tgcctagaac gaagccggca aatgctccac caacgccaag tacccagttg atcccaccga
    19201 ccatgccatc agaaatgaaa cctgctaaag gcatgatgaa gaaaatttca atcagaccga
    19261 tggcaatcaa tgaaagagtt ggtgtaacga tgatatctac cgcatcaggt actactttat
    19321 gcagtttttt ctcaacgatt gacaatagcc aaacagcgaa gatgacacct aggatcccac
    19381 cttgtccagc tgcaagagct tcaccagtaa ataagttttt cagtggtgct tccggattca
    19441 tccctgtcag catcacaacg gccccgataa ctccaccaag agttggtgtt gcaccaaaaa
    19501 cttgtgcagc attgatacct gtatagataa ctaggtagct aaacatcccg tttttcaaga
    19561 tgttcatcac atcgatatat tgttgccatg ttgctgcatc aagtgttccg gctgtgacta
    19621 agtttgctaa aactgcggca atccctgcca cgatcccagt accgacaaat gcaggaatca
    19681 ttggcacaaa gatattagaa atgtctttta ataccgattt gattttagat ggtttttgtt
    19741 tcgatttttg tgccgctttc atttcttgtg cccgttcgtt tacacggtct tttccagatg
    19801 ctgatgaatt tgctgtagct gtacttacag aagaaagttt ttctcctaat ttcacgcctg
    19861 ccaaatcaac catttgctgt gctactttgt tcactttccc aggtccgatg atgacttgaa
    19921 gctgttcatc ttctactaca ccaagcactc ctggaatcgc ttttaattca gagacattta
    19981 caagatcatc attgatgatc ttcatacgca cacgagtcat gcagtggaca atgctttcta
    20041 cattgcccat gccgcctgcc cctttataaa tttgttttgc cagttcttcc aaagtcagtt
    20101 cgttttttgc catggtcttt tccccctcat aaggtccctt ttacaaatcc gtttgcttca
    20161 gctaattttg cttgagcttc ctctttatca ctatttgtca aaatcatgac gatagccagc
    20221 ttaacatttt gatctgcttc ttcaaagtac ttttcagctg tttcgtaatc agcttctgtc
    20281 gcttgcatga tgattcgttt tgagcgttcg attagttttt catttgttgg tttgacatcg
    20341 accatcaagt tattgaatac tttacctata ccaatcatac tgatagtaga aatcatattc
    20401 aagataagtt tttgtgctgt tcctgacttc aatcgagtag agcctgtcaa aaattctgga
    20461 cctgcatcga cttcgattgg gaaatccgcg tgtttactga tttcggcttg tttattgcaa
    20521 gaaatcgttc ctgtgacagc accgatcgat tttgcgtaat ctaatccacc gatcacatat
    20581 ggtgtacgac cgctagctgc gataccaata actatatcat tttttgtcaa attcagcttg
    20641 attagatctt cttgccctag ctcataagaa tcttcggccc cttcgacagc tacagtcatt
    20701 gcttcttctc caccagcaat tagacctatg accatttccg gttctacacc gaatgtcggt
    20761 acgcattcag cagcatctaa cacacctagt cttccacttg ttccggctcc catataaatc
    20821 agacgtccac cttttttaaa ggactcaatc gtgttagcaa tcactggttc gatacttgtc
    20881 agttcttttt caaccgcagc ggccactttc tgatcttctt tgttcattag ttgaacggct
    20941 tgagaaatac tcatctcatc taatccaaaa gtttcttggt ttctgcgttc cgttgttagg
    21001 ttctctaaat tgattttgct catttttatc ttccttcttt tcttataaat tgatagttca
    21061 taccagcttt gatttggttg atcaatggta gctcttcttt gataacttta gctactacat
    21121 tcactttttc atccgctggc aaatcatatt tggttaattg gatctcaccc atatagcgca
    21181 aatacttttc gttatctaaa gtaatagacc ctttcatacg cgtagctgta tttcttgctg
    21241 ggataggcgg aatctctcga aaacgtgcat ctacgcttcg tatgacatct cttgcatcgt
    21301 cttgccgatt cgtatgcttc cctaatatat attcgtaaaa ttcttcatct acaggttccg
    21361 tatggagcaa gactgtctgt tctttttgga aagaagcaaa ttgctcctga acgttttctg
    21421 acagacctgc atctccgatg taaacaatat ccgtgttact tgctaataga tctagagcgc
    21481 ctgcaagagg atggatccct cgatgttctt ccaaagttgg caatccttga tatagtgggc
    21541 ctctcaactc tgtatctcct ggtacaaaac cttggacaag gagaccttga attttgagcc
    21601 attgattttt tttctgatac cattctttgt ccagccctgt ttcgggacgt ggataataat
    21661 tatgccaagc ttcaatctgt gagaaatcag cttctgcttc tttcaattca tcgatatctt
    21721 taggcgtgat cgtgctagca ttcaaactga ttttcaattg atgtgaccag tcggcaatct
    21781 gctgattgga aatatgataa tccattctta aacctgttac accaattgct ttcaaaggcg
    21841 ttagatcttc taatgaaaaa cccgctcgtt ccaatgcctc tcctgaaata tcgaccatca
    21901 attctagttt attctttttc gcttcgcttc ctagttcgat caatcgcttt tgatatgccg
    21961 aaacatcatc ttctggtata tgcatggaag tgaaaatacc gacaaagcca ttggatgcca
    22021 tttggcggat atactgtttc ttctcttctg acaaagaatc gttcataaat attgaaaagc
    22081 caaacatatt atccctcttt ttctgtatcc gtcttcatt
//
LOCUS       DAZKOW010000046        21923 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000046 DAZKOW010000000
VERSION     DAZKOW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.046"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            282..521
                     /locus_tag="ACG912_001596"
     CDS             282..521
                     /locus_tag="ACG912_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512921.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(883..1143)
                     /locus_tag="ACG912_001597"
     CDS             complement(883..1143)
                     /locus_tag="ACG912_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512922.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1328..1825)
                     /locus_tag="ACG912_001598"
     CDS             complement(1328..1825)
                     /locus_tag="ACG912_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFQ0512923.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1955..2665)
                     /gene="budA"
                     /locus_tag="ACG912_001599"
     CDS             complement(1955..2665)
                     /gene="budA"
                     /locus_tag="ACG912_001599"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFQ0512924.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="ACG912_001600"
     CDS             complement(2678..4342)
                     /gene="alsS"
                     /locus_tag="ACG912_001600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFQ0512925.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4547..5209)
                     /locus_tag="ACG912_001601"
     CDS             complement(4547..5209)
                     /locus_tag="ACG912_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0512926.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5219..6034)
                     /locus_tag="ACG912_001602"
     CDS             complement(5219..6034)
                     /locus_tag="ACG912_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0512927.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6296..6739)
                     /locus_tag="ACG912_001603"
     CDS             complement(6296..6739)
                     /locus_tag="ACG912_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFQ0512928.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6873..7211)
                     /locus_tag="ACG912_001604"
     CDS             complement(6873..7211)
                     /locus_tag="ACG912_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0512929.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7199..7576)
                     /locus_tag="ACG912_001605"
     CDS             complement(7199..7576)
                     /locus_tag="ACG912_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0512930.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7800..8993
                     /locus_tag="ACG912_001606"
     CDS             7800..8993
                     /locus_tag="ACG912_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0512931.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9159..9581)
                     /locus_tag="ACG912_001607"
     CDS             complement(9159..9581)
                     /locus_tag="ACG912_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512932.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9976..10170
                     /locus_tag="ACG912_001608"
     CDS             9976..10170
                     /locus_tag="ACG912_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512933.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10167..10661
                     /locus_tag="ACG912_001609"
     CDS             10167..10661
                     /locus_tag="ACG912_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512934.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10823..11995)
                     /locus_tag="ACG912_001610"
     CDS             complement(10823..11995)
                     /locus_tag="ACG912_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFQ0512935.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12201..13016)
                     /locus_tag="ACG912_001611"
     CDS             complement(12201..13016)
                     /locus_tag="ACG912_001611"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFQ0512936.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13166..13612)
                     /locus_tag="ACG912_001612"
     CDS             complement(13166..13612)
                     /locus_tag="ACG912_001612"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFQ0512937.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13609..14124)
                     /locus_tag="ACG912_001613"
     CDS             complement(13609..14124)
                     /locus_tag="ACG912_001613"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFQ0512938.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(14197..15111)
                     /locus_tag="ACG912_001614"
                     /pseudo
     CDS             complement(14197..15111)
                     /locus_tag="ACG912_001614"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(15108..17435)
                     /locus_tag="ACG912_001615"
     CDS             complement(15108..17435)
                     /locus_tag="ACG912_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFQ0512939.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            18044..18190
                     /locus_tag="ACG912_001616"
                     /pseudo
     CDS             18044..18190
                     /locus_tag="ACG912_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            complement(18278..18571)
                     /locus_tag="ACG912_001617"
     CDS             complement(18278..18571)
                     /locus_tag="ACG912_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFQ0512940.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            18608..18793
                     /locus_tag="ACG912_001618"
     CDS             18608..18793
                     /locus_tag="ACG912_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512941.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            complement(18974..20152)
                     /locus_tag="ACG912_001619"
     CDS             complement(18974..20152)
                     /locus_tag="ACG912_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0512942.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(20256..20570)
                     /locus_tag="ACG912_001620"
     CDS             complement(20256..20570)
                     /locus_tag="ACG912_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512943.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            20677..20892
                     /locus_tag="ACG912_001621"
     CDS             20677..20892
                     /locus_tag="ACG912_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512944.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(21192..21434)
                     /locus_tag="ACG912_001622"
     CDS             complement(21192..21434)
                     /locus_tag="ACG912_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0512945.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(21421..21849)
                     /locus_tag="ACG912_001623"
     CDS             complement(21421..21849)
                     /locus_tag="ACG912_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFQ0512946.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         6454 a         4313 c         3706 g         7450 t
ORIGIN      
        1 tatcctccta gcaactattt aaatacccaa aaaaattacc tctagataat atcataccta
       61 cgttttcact tataaatttt ttcataaaaa aagcgtttag aggcataatc gttaatacag
      121 atggtaacca atgtatctgg tttgaactgg gatatcaccc tctccatata tgtattacta
      181 agactaatag atactataaa agacttgata gttttgcatt gattttgcct aatactgagc
      241 ataggtgtat gctaaacgat actataagga gtgtgtacat aatgaaaaga attctgttta
      301 tcagtttatc aaatttattg ttacttactg cttgtgggac ttctgcatca aagaacatac
      361 cttcagatac gcaaatttcg aatagtatcg attcaacaat ttctacaaat gagaaaaatt
      421 ctccggtaga aacttccgat ataaaagttt ctacctctgc aaaaaacagg gtaaagaaac
      481 tgtcagcacg tctcctttat ttcaaacaaa ctctgaatta gcttcttcat cttcttcagc
      541 aaataatgat gatagtttcg cgtttactcc taactaaatg caacctttac aaatgcttta
      601 cgataacaag cctgagctaa atgatcctga tatcagtttt gcattttttg aaatgattgg
      661 atcggatgat ctttttaaaa ctactagttt gtctatccga aaataggaca gttctggcag
      721 tataggtttt tatcgagttt ctccaccagg cctcgttaca gagacagatt ctctaggcga
      781 accttttgaa taaatcaaaa atcatgaaca atcactgcca actaaataaa gtgccttcaa
      841 aagcttggtc ttctcgcttt tgaaggcact ttatttaaat ctttatgaaa ctaaagtatg
      901 ttcatatttg ttgctatagt tatatagata ttctgttgtt tccaactcat aaacgtgagt
      961 atattttgcc actacttttc cgcatttttc taatatttca ttgcgatcga cgtcatgata
     1021 ttcgtctacg cagatcacaa cacaattttc taatttactg attacttctc caaccacttt
     1081 ttgtttcaag ccaataggtt ggcaaatata tttttttcct aaaatgatag tttcgttatt
     1141 catatttcat ccatccttaa tgtttttcca ttatttaaaa tgataatacc attaaaatat
     1201 gattatgaaa atcaataaca cttgtgattt tataaaaaaa taaaaaaacc gaaaaacaga
     1261 aatgcccact gtctttccgg ctctttttat tcttgatttc tcttcttttt tgtttcaata
     1321 gacgatttta gttattaata aatgaaaaag catttatatt aaaatttgct aataaaagat
     1381 ctacatattt ttctagaaat gcttgatcga tttcatcata ccctttcgtt actccgcaat
     1441 cgatatctaa aacaccgatt aaattacctt cttttaccat tggcactacg atttctgatt
     1501 gtgcggctga atcgcaggaa atgtaatttg catgggtttt gacatcatca acgatcaatg
     1561 tttcttgttt ttcagctgat tctccacaaa ctccttttcc catcttgata cgtgtgcaag
     1621 aaactcgccc ttggaacggt cctaaaatca attcattacc atcgaataaa taatatcctg
     1681 caaagacagt ctcaggcaaa gtctcattca acaatgcaga tgaatttgct aaatttgcta
     1741 accagttatc ttctgcttct aataatcctt gttgctgtaa taataaaatt tcgtatgcat
     1801 cttttttctg tttttctgtc cacatgataa aaaccgctcc tttttgatct atggagaaga
     1861 ttatagctca gtttttttgt aaacgctagg tttcctcctc aaaaaaacga gatacgtcag
     1921 gttaatcacc ttgaacgcat ctcgctgtct ttttctattc tgcttgttgg atttctgctg
     1981 ctaaatcagc ataatcaatc gtttcgtgaa caaaagtcgg atcatcaact ggaaaatgct
     2041 gtacaaggga cgagatgcgg ctaatctcaa cttttccttc agccacttca aaatccatta
     2101 catgtccgcc aaatgtatgt gtatcatcga taaaatgcaa gtgaaatccc gctgcagcaa
     2161 ctccttggaa taattcaggc gtaaagaacc cgactaatgt tccttggaca ttgctttgtt
     2221 ggaattctgg ttgagactct gaaatttttg ctagtcttct gtaaggtttt tcttgcttcg
     2281 gcatgatacg gatgtgcata tttttgaata atccagttat cttcactgcc aaaaataggt
     2341 tttccccggc ttctcttact aatatgtctt ttttcaattg ttctccatct gcagtttttg
     2401 agacagaaaa actagtatct ggcgtaaaat ctgtaattgc tgcatagggg gtaagttcac
     2461 ttccatctaa ttgggcaact gagccatctg atcttccttg ataagctacg ccatctaaaa
     2521 agatgacttc accatcaagc ccatgcaatg ttcccaatcc cagagttcct ttttctaata
     2581 tatgagcaat cgtttctgtt ccatccatca agccagccat caaagctcct aatgtcccgt
     2641 gctggtataa gattttttca gtcatgtcga cacttcctta atagaattga tctggtaaga
     2701 ttgtttctcc caatttctca ttatcacggt aatcaatcgg aatatcaata attacgggtc
     2761 cttctgtcgc aaatccttct tctaaagctt tttctaattc ctctacactt gtagctcgga
     2821 ttcctttagc gccaaaagct tctgcatatt tcacaaaatc aactggacca aagtctacac
     2881 ctgacgcccg ctgatatttc attttttctt ggaactctac catattgtag tgtccatcat
     2941 tccaaatcaa atgaacgata ttcaattttt tacgtaccgc cgtttctaaa tcctgggcag
     3001 agaataaaaa tcccccatcg ccggaaactg atacgatttg tgtatttgga cgcaccaatg
     3061 cagcagaaat cgcccatggc aatgcaacac caagcgtttg cataccatta ctgaataaca
     3121 aatgacgagg ttcataactt ctaaagtggc gtgccatcca aatataatga ctacctacat
     3181 ctacggtaac agtcatatca tctgttactt ttgactgaag cgtgttaata acttctaaag
     3241 gatgcaagat acctgcttct ccttttgatt gaacaatgtc ccgctccgtt aattctgctt
     3301 gaagagatgc taaatattct tttgcatctt ctgacacttg ttgaggctct aatgaacctg
     3361 tcaataaatc aagagtggct gagatatcac cgatcaattc tctttcaggc tgcataaaag
     3421 gatcgatttc agctggcgct tcatcgatca caatgatccg cgcatctttt tccgcattcc
     3481 agtttcttgc ttcatattcg attggatcat agccaatagc aataactaga tcacttcgtt
     3541 tcaaaagcat atctcctggt tggtttctaa ataaacccac acgaccaaag aaatgcgctt
     3601 ctaattcacg tgagatcact ccagctgctt ggaatgtctc tacaacaggc aatgctgtct
     3661 ttccaactaa ttgcctgatg gctaacgttt ctttttcact tgatcctcgc atacctacta
     3721 atagtaccgg tagttttgct tctctaatgc gttcagctag gtaacggata tcatctgcag
     3781 aagcagaacc taatttagga tcgctcaatg gcttgatgac attcccttgg acaggtgcat
     3841 ccactacatc ttgcgggata ctgataaagc tcgcgccttt ttttgaactc ttagccatac
     3901 gataagcatt ggcaatcact tctgaaattg tttctggatc ttggatttct gcactgtatt
     3961 ttgtgattgg ctggaacagt gctgcattat ccatgctttg atgggtcaac ttcaatagat
     4021 cgcttcgttt tacttgtccg gcaattgcta aaactgggtc accttctgct gtcgctgtta
     4081 ccaaacctgt agctaagtta ctagcaccag gaccacttgt tgcgataact actcctggct
     4141 ctcctgtaat acgtccgatt gcttgtgcca taaatgcagc attttgttcg tgacgagtca
     4201 cgattaattc aggaccttga tcctctaatt cattaaatac accatcgatt tttgcacctg
     4261 gtataccgaa tacataatca acatgatgat taatcaaact ttctactaca gatttcgaac
     4321 cttgtttcat tttttcgtcc attgaacatc gtccttttta tgctttattt ttaattatta
     4381 gttagaaata atcacaaata attgatacag tttctaaacc tgtttcattc aaaagtatag
     4441 cacgttttat ttcatttgtt tagccatatg tttcaaaaaa tgaaaaagaa tgacagtttt
     4501 tacataaaaa ctgtcattct taattgaaag tttcaattta taaagtctac agatccatag
     4561 ttctcatcaa tttacgattg ttgaagtaaa tcaaaccaaa tacgatagaa ggacctagta
     4621 gaatacacca ttggggcttt acgaaaaaga agagaaacaa acttgtcaat agtaaaaaat
     4681 ctagtaaact cacataccat tttttcaacg tatagtagaa cgctatccgt agaacttgat
     4741 ttggtttcga cgctggattt ctcacctgaa aataccagta gttgatgcta aaggaaactg
     4801 ataaacaagc aagtaaaaca aacaacggaa tcaaccattt tccataagaa aaacgtataa
     4861 aaaagaaaca atcaaaaatc attatcacac taactatcca agcaaatagc caataaaaaa
     4921 atccctttag ccaaaattgg cgaaacgtct ttatgaacaa acgtactggt gaaaaacttt
     4981 tttcttttgc taattcttct gtcataccta tcatcacgat gcaacctgga ccaataaaaa
     5041 gtaaagaaaa aataaaggaa aagacattcc ttccatctat cgacaaaagg aacgcagctg
     5101 ctatatacaa gatattgaca caccaaaaac aaaggctact tgccaatgat tgatacaacc
     5161 aataaaagaa ttccatataa acatttttgt caaaacgctg ctctttcata atcacctcct
     5221 attccttcat tcctgatgaa gcgatccctt ctacaaaata tttttgcatc gctaaaaata
     5281 taaaagcaac aggaagaaca gaaacaactg aggcagccat gattaatgcg tactctgcat
     5341 caaatagccc aataaacatt ttcaacccta actggattgt tttattttcc gtagaagaca
     5401 aataaatgaa tggccccata taatcgttcc atgtattaac gaatgtgatg atcgtcaaac
     5461 tggcaatcgc cggttttgta agaggtaaaa tgatcttgcg atagattccc cactctgata
     5521 agccatcgat ccgcgcactc tcacataaag aatctgggat actactgtag tattgcttca
     5581 acaagaacac accaaaagca ttgaacgctt gtaatagcac caacgaccac aacgtatatg
     5641 ttaaacccac ttttctcatc ataatgaact gcgggatcat ataagactgc caagggacag
     5701 caatcgtgcc gatatatgct aaaaataaaa tatcgcgccc tttaaaattt agtttagaaa
     5761 acccgtaagc agcaaaacta gaagtaaaca actgaatcaa cgtgatcaca acgcttaaaa
     5821 aagctgtatt cttaaaaaat gtcactaaag gaatacgaga ccaaataacc agataattct
     5881 ctaaatgcca agttttaggg atccattgaa tcggaatcgt gaacacttca ttatttgctt
     5941 tgaatgaagc agaaaccatc cataaaaaag gaatgatcgt caataaagct aatacaatca
     6001 gcagaatcat acccaaccat tgaataaagg gcatgttttt tgaacgaatt tttgcttttt
     6061 ttctttcatt tcgaacacct ccaaagttat aatcttcatt tctttgtaat gattacaatc
     6121 cagaacaacg gttattatag ctgattcatt tccgttcttc ttatgtttgt aagcgcttat
     6181 ttaacaagtt atgtaaatct ctttattttt tttgatttaa aaatgaaaaa atacaaaagc
     6241 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6301 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6361 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6421 ctcgctttga aagatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6481 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6541 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6601 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6661 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6721 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6781 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6841 tttctagtaa tagtgccaat gtattcgaat tatcagcttc cacacagccg atcgtaaatc
     6901 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6961 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     7021 cggttttcgc gtacaaacag ttctgttcct agttcctctt ctaactcctt cagttgtcgg
     7081 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7141 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7201 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7261 gtaaacgttc caaacactct tgcatttcag caatttcttt cgtcaaaagt tcactttctt
     7321 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7381 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7441 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7501 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7561 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7621 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7681 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7741 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7801 tgacaacgta tcagcgaaag gtattattat cgacatcggc gggaattgct ttagagaata
     7861 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7921 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7981 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     8041 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8101 ttcgctttat cgcaggtgta ggagcgggag gagaatacgg ggcgtgtatg tccttgattt
     8161 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8221 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8281 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8341 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8401 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8461 tccaaattgc cggctatttt gggttaatga attggctgcc ttcaattatg caaaatcgat
     8521 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8581 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8641 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8701 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8761 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcatta
     8821 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8881 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8941 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     9001 gtctgtaaat gacaagaaag aggtgtggca aaagcttgca tctgcggtaa taagaccagc
     9061 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9121 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9181 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9241 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9301 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9361 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatatttt
     9421 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9481 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9541 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9601 actttactat actacataca agaaaaaaaa cggatacaaa tatcatattg tcacaccgtc
     9661 aatagtgcat aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat
     9721 catttaatcg tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt
     9781 attattgcat acttaataac agaactatag caaaaaatcc cgtgtatcaa cacgggatta
     9841 gattaagttt cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt
     9901 gaaaaggaca tttattgaaa acggttgttt tttacttaag gaggaaattt agcgaaaaaa
     9961 ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct
    10021 actactcaaa gcacaagttc agatcaggct tccagcagca gtaccacgat ctccgaaagt
    10081 acggcagatt catcgaaaga acaagaacaa attgaaaaga aaaaagaaga gaaacaaaaa
    10141 gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga atctgctttg tcacaagctg
    10201 aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc agctatccaa gcgctacctg
    10261 aaaacaaaca agagttgaca agccgtcttg ctgcaattga ttccacaatc aaagcaaaag
    10321 aagcagaaga agaacggaaa aagcaagaag aaattgcacg acaacaagca gaacaagcgc
    10381 aacaagaagc aatcgcacag caacaagcag aacaggcgag gcaagaagca atcgcacagc
    10441 aacaagcaga acaggcgcga cagcaatcag cagctgaaca ggcctctcag caggcacaag
    10501 ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt cacaccgacc ggttcaaaat
    10561 accatacaca taaatgtgga aatggtacgt atacccctgc cactttaagc gaagctcaag
    10621 ctcgtggttt aaccccttgc tccaaatgtt atggaaacta attcaaaagt tatactataa
    10681 taataaaatt cttctgaaac ataaaaaaat agaagatggc gttccagata gtaaaccaaa
    10741 aataacagtt tacacgtcca tttcgtcagc ttctctcttt tttcatttag tctgttccct
    10801 ctgctttttt ggacatcttc attcattctt tctcaatcaa atagtacacc tctttttctt
    10861 ttcgcagttt atatcttcct tttttcaatt tcattgaaaa ctgtttcgaa tgtttatgtt
    10921 ttacatggat tttaagcagg agctcctcat gttgcaagcg atagttcctg ccttttaaat
    10981 cagaaaatct cactagtccg tattcatcag aagctttcac aaatggaact ttttctctag
    11041 taatatagtg tttgccgttg agcgttttta cagaaaagac cattccttcg attggtcgtc
    11101 cgtttgtgtt ctttacataa aaagataaag gatagtttcg cttagcgatg gccaaagaat
    11161 ctaaataagc gatgagtagc caaactaagc cgattgatcc aagtagtata ccgataaacc
    11221 aaataaaaaa tttccatttt ttccattgat caattccttg gatcgctttt tttgcctctt
    11281 ttgcttgata aggaatacga tgccccgtca caagcagtcg atgtgtattg accatataag
    11341 gagtacaggt aagcaaagtc acgagatctc tgcctttttg gatacccaaa gaatctactt
    11401 catcaggtaa gaccacttgg attttttcta cttgatacgc caatgtcttt ccattgatct
    11461 ggatgaaaaa ctggtctcct tttttcaact taggcaaatc tgtaaataat tttgcttctg
    11521 ggagtccacg atgcccagaa agaacggcat gggtgttttt cccgccagtc ggataagaac
    11581 tttcttccaa taatgacgtt cctttttgca aaaatatttc cgttgtctga tcgaatatag
    11641 gtaagctgac attgattttt gggatttcga tgactccaag catatgttcc ataaaaaagg
    11701 cattactagg taaatctttt aatgcttttg catcaactgc ttggttgaaa ctgcctatcc
    11761 ctggtgatac gttttgctct gccaactgtt ggtttttctt tgtcattttt tcctgctgtt
    11821 tttttatttc ctctgaattc ttattcgatg cttgcttttg ataatggatg atcatttgct
    11881 gggcaagaaa atcattcagt gaatcttcta caaaaggata agaaaagatc ccgatcccaa
    11941 cgactaaaat gattgccatg aagatgtcca gtattcttat tctgctttta ttcatcatgt
    12001 ccacctattt tctaatatgt atatgaagag gttgtgaaat cagcgccttg acctgagtat
    12061 tagcgaagca aacggcaaaa tagttcctcc tattttgccg tttgttgagc gaataccaag
    12121 agaccgacca cttgtcacaa cctttctccc catttgaatc actatcgttt atttatcgta
    12181 tgatttttgt ttccaatagt ttaagcttct gtttcttgat ggcgtttttt gaaatagatc
    12241 actgctcctc caactaaagc agtacctacc aagatgaata cgatgatccc cataccacca
    12301 gttgaaggca agattccttt aggttggtta tcgatcgttg tttcatcaga agttgttgga
    12361 tcatcatcta ctgtaaattc agtgtacgca gcttcattct taggtaatgc gtagccttct
    12421 ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca gcccttcgat tgaaaatttc
    12481 ccttctgaat cagaaatcaa tttcacttct tcccagtcag aacctgtgac ttcatctgac
    12541 caagcatatg ggtattctcc tgaggcaagg tttgctattt ttttcattaa tttaacagtt
    12601 ggatcttttg ctcttagaat agaaaattct gcatcggcta atttttcttg cgtgttcaca
    12661 tcttgtttga tgaagtggaa atcaccgtag ttttggacat tttctacagt aatcgtgtta
    12721 tccgtagttg tattttcccg attgactaca ccgtttggat tcccaagatt ggcttcatct
    12781 gcgccatgga tgataccggt tgtttgttgc aattttccgt tttttactat aaaagtagtt
    12841 tctttttgtg gattggttaa ttgataacca tctggtgctt ttgtttcaac taaagtataa
    12901 ttgccgtcta ttaatccatt gacactgatg gatccttcag tatcagttgt gaatgtcaac
    12961 gctgactcag caggatcata gagagcgaca ccagtgtttt catcaaaacc ggtcacgaat
    13021 tgattctctg aatttttgat ctggaattgc gcccctgcta acttatctac ccctgaaaag
    13081 ttcccagctt tctggatttt gatattgctt tctttgatga catttttagc atataggtga
    13141 atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa cacgatattt ggcgacgttt
    13201 cgacaccggg ctttggtgtt tctacaaaga cgtatacagc atctttccca tcagatttac
    13261 ctggaagagt caggttagct aatccttcac cagaagtcgt tacacttgcg aagggtgtag
    13321 cgtctgctgg aactgctgtg tctttacttg gatagtacgt agcagtgatg tcatatacgt
    13381 tgaattcaac gccgttcaac ggttcgctat tcggaaatgt caattccgtt ccgttgttta
    13441 tttgattttc tggtgctgaa gtgaatttca atttatgcaa attaaccgta acattttctg
    13501 gtgtaggtga tacggctgca gaaacctctg cagttcctag gccgagaacg aaaggaagca
    13561 gcatgatagc agttcctaaa aacgtccata tttttgtttt gatgtttttc attttatttc
    13621 cctcctattg tttccttttt gttgtttttt ataaaaccaa gctgtccctg ttgaaaggac
    13681 tagcaataac cctaaaattg agaaacctcc aagcatctct tcgcttgttt ttggaaagct
    13741 tcctccattt ttttcatttg atggaccatt tgtctgcatc tgaactggcg tttttccatt
    13801 attcgtcgga tttgcttttg gttcgttgat gatttccaat ggtataggat gaagcggatc
    13861 ataactgcct ttttcaactt caaagagaat ggttgattga ctcaatatat atcctttagg
    13921 cggtttaatc tccactagtt cgtaacttcc aaaagacaat ccatggacag aaaatgttcc
    13981 atctttactg gaagtaaaaa tcgttgctct tgaatcatct ttggctaccc aatgatttat
    14041 cccgttttct tgcgctaagt attggtcatt ttttttgact agaaattgag catctgctag
    14101 tttttgtttc gtttgccgat ccactttgac aaattgtttg cctcctgttt ggatggcaag
    14161 atgtgtttct actgtttgat tgtcagtcgt caaacgtaca tcgttatcaa atgtcgtttt
    14221 ttcactgttt tctttcaatg tcatttggta acggatcgtt aatttttttc cagcaaacga
    14281 actaagatct tttccagaat ctgataagac aaaaccatgt tttatttttt gtgtgtgaaa
    14341 tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt tctggttcta gccataactg
    14401 aggatcagct gtatctttga tcgtataggt tcctatctcg ttgatgttct caggaattgt
    14461 tacggtgatg agataaggga ctttatcccc atctgcaaaa tctgtctgct tattctctac
    14521 tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg ctctttggat acaagtggat
    14581 ggtcgtcaat ttatgattag atgaatctgt cacaggaagc acaacgacaa atggtgctga
    14641 tttttctttt acttaatctg aagtctttat ttccgtaaat cggtaaacag cgtcacgacc
    14701 ttgctcatct ttgtccgatg cactaaaaga agcaatgccc tcttcctcgt ctactgtttt
    14761 tgtcacattt tctgccagta ttttttcact atcacttttt tgtgctagtt ttctttgtgc
    14821 gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat tcatccgaga catcatatat
    14881 agcgaaagta acgccgttca acccttttac tccttgaaag ttatccggga tatttattcc
    14941 gtcgttttga ctgatacttg gcatcttatc gctttcgaac attcgattat gcaaagtaaa
    15001 tgtaataggt gattctctag ctgacacagt cggagaacct ataaaaaaga ccagtaggat
    15061 aaaaaagaga acagcaaata tatccacacg tacatttttt gtttttttca tcgctgttca
    15121 cctgcttttt tcttacgatg gataaaataa aagaagccag aaaaaataaa gatcagtcct
    15181 aaaaagacaa agggcagtag acctatccca cctgtcgaag gaagcaagag agtcggattg
    15241 atcgtcaact tagcggtatc tgttgcgata atcgtataat tattgtttac acttccttcg
    15301 cctaaactaa tcgttactat ttgtgatcca atatctgtcc ggctgttaac tgttagggta
    15361 taatccaatg attcgcctaa gacaacaccg gtaacgtgga gatttccaac cgaaatcgag
    15421 tgactttggg catcataatt aaaggcagcg tcatcgacag taatcactgc gttagcttta
    15481 tagatggtat aagttcctga tgtgttggct atatcagtaa atgacaactt gggatttgct
    15541 gctttgactt tgtctattcc gctattcgtt agtttaattg tatagctgcc agcattaacc
    15601 atattttttg ctgattgtgc atctgctgtg taatccatac tgctaagtgc cacttgtact
    15661 ggagtaagcg aactagtgac accctctgct agcttgacat ggattgacgg gacacctgct
    15721 tttccatcat atgtcttact gccactgagc tctaccgcac cgctgatttg cttaatcgta
    15781 tagacatcag atgtctgcac gatagtaaat aaatagtttg ttctggcagc tagtttagct
    15841 agtccgcttt ttgtcaattg ccaagacgta taggttccaa tttcagtagg cgcagttact
    15901 tcaactccct tttcattcat cgctttgaaa tcagttggat tccatgtgat ttgatcaata
    15961 ctgagatcat tgtttaaaag ggatgttgat tctgttttat cattaaaggc atatttaacg
    16021 atcggtacaa cagaaagacc atcatagtac ttactgccat ttgcattgat tgatactgtt
    16081 gcatttgtct tataatatag gatgacttca gaaatctgtt ttccatttgc gtcctttacg
    16141 attacatttc catttgtatc taaactagct aaaacactca ttgctgattg gttctttgtt
    16201 tctatcgctg aaacagctgt aaaggtatat ccgttgatag taggcgtatt agtcgtcagg
    16261 tcttgtgcag cacttccgta agtaagattc attattttag aaggagcaat catctgtcca
    16321 aaatagtctt gatacttaat agtgattggt gtttgtttgc ctgtataggt caccgtatcg
    16381 acgttacttt ctagggctgt gaaagaaccg tttgtttgtg caaaagaata gcccgttact
    16441 gctgttggag tgataaccac tttgtcaccc acatggattc cagataatgt agtcccgtac
    16501 gttccgattt ttgctgctcc accattgact gtataagaaa aagttcctgt ccgattcgca
    16561 ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg aaacatttcc cttaccatca
    16621 acgatctgcc catttttgat cgagtacccc gtgaatggaa tatcatttgc taactgtata
    16681 ccgattgaaa aagtactcat acctcctccg gcaggaagcc ctccccatgt tgagggtgta
    16741 aaagaataac ttttatccgc attgggtgta actgaatcga tcgtatttcc agcggtagcc
    16801 acccccatat agatactttt tgggaatgtg atagtagtac catttactgt ggagccaata
    16861 aacagattat ggatatctgg acggttatca ataaaagcga ttgctttggt tacgccttgt
    16921 aacgcagttt tatccgcact catcgatccg ttaaccgcaa caatgactcc atctttggca
    16981 tatatcactg tatatccatc tggtatggta tgtccgttga aattcgcagt ggccgcaaca
    17041 gtactaaatg tctggcttct acctgtttct gaataagctt tctcccctgc tgcttttgct
    17101 gctgtgattt ctttttctgt cggatcaatc agttgagtcg aggatgtggt tgaggttgtc
    17161 tcgttgttgc tagactgatt ggtgctttct atggactgta tttcagaata gggtggacta
    17221 tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt ctgtataaac gtttacataa
    17281 atacttgata agctccctaa gaaagtaaaa atcaagatga tcgaaatcat aatgggatgt
    17341 aagtattttc ttttagatac agctttgagt ctagaaacaa aatgtttttg acatatacgt
    17401 tggattttat tttgagtttt cttaatcatt ttcatccctc ctctcaataa aaaatcatca
    17461 aataatcctt tacacgttat tctaaattta atttttatta ttttattgat gtatagaaag
    17521 tttggggctt tttttctctt tttattctaa ggttatttta gtcttgtcct ttaatacaaa
    17581 gattttattt ttaaataaaa aatactcttt ttttacgatt attgaaaaaa aaagataaaa
    17641 cttgattaat tgaagtattg cttaaatttg gaagctatga gggaagaata aaagagttag
    17701 gacgggatta tctactattt ttctttgttt taaatatttg ttatctttga acataaaaga
    17761 gaaatgtgtc gactgcagtg ttgtgtccct catcacgaat aaacggtgtt caactagctg
    17821 atcttgtatt cacttaaaaa atggtaaagt acgagttgct attccccatt ttcaattttc
    17881 taacgctcag gacaaaatac tgttttcaca acctcattgg aataaaagca tatatattgt
    17941 ttcaagaacg aaatcttgtt atctttttaa agggcatctg tccaaaatct aattttagga
    18001 ggtacgtttt ggtatttatt cgtacaaata aaagagaatt atctttattc atgtaacgca
    18061 tccaattgat tttttcatgt acgctcctgg aacgatttta ctgacgattg cttatttaac
    18121 tgcgaatcgc tcattagctt ttatcatagc tatccatatt ttgaataacg ttcttggctt
    18181 tgtattatag atacagatga caaaaaaagt tcaagtatgc ggtgtagaac caatatcgca
    18241 tacttgaact tttttcaatg agtataaatt aaaacttcta attttcaatt cccatcatct
    18301 gatatgcccg accgtctgaa cttcttttga aaaagccaaa tgtgattaga taacgtcttt
    18361 gttcgacata atcgccacta gaaaatacgt tcagcagtat cgaattaacc tctgtttctg
    18421 tataagtcac tccatggtca aagcgtttca agacttcttt caataggatg tatttctttt
    18481 ttgttgaact tggtacttgt tttacttttc catcattcat gaatgtgtct aaaactttca
    18541 tcttttcttg ctccgtataa gtataaacca tcttctttct ccttttgatt ctaagaatac
    18601 tccatttatg ttagaatatt ctggattttt tattaaagcg gttactctta agaaagatat
    18661 tatcatagtg ctcttttttt tactactact tataggtaga tttattctaa tccattattc
    18721 tcccctacca ataattaaag aaaaaactgg atcagggtgt tcaaattaca tgctaagaaa
    18781 aaagaaccct tgaataccaa gggttctttc tcagtctgat aacaatgatt aacgacggat
    18841 ttctttgata cttgtgaaaa gtcgttaaag tattgatata aaaggatttt tattacctct
    18901 gactacatat tgactaattt gttaaaaaat cgtttttcaa gaatcctgta ataccagtgc
    18961 tttcaccgtt tttttaactc tctaaagaac taaaagcaat cagaggtttt accttttcat
    19021 ttgacgttaa gtggcgataa attttcgaag ttgtttggtc agtatggcct aagcgatctt
    19081 tcaagacttc ccagctacag tgttccgcta ctaagcttgc cattgtatga cgagctaaat
    19141 gagtatgaaa tggcttatta attcctgagc gattcacgca ccgtttgacg tgatcattta
    19201 catactctcg acgataagga acaccacgtt tactagaatc aagatacaca aacaaatatg
    19261 tttcttctat aataaacctg taatgttgcc tcatctcacc caaagcctta atcaactgtt
    19321 gtagcttttc cataactaga ggagttacta gaactcgtct agcagaacta actgtcttag
    19381 tagtttcaat tcgaaaatcc ttactcttac ctttaactgc cgtttgctta tagaacagaa
    19441 tttcatttct tttgaaatct atatctccag gctgtagagc aagcgcctcg ctgactcggc
    19501 agccagtgta aaatagaaca tggaaaagta cagacatcgg taattcctca tgaacaatgc
    19561 cataattcaa aaaagttctc aattcatcga cagtaagaaa cttgtcctca atattattta
    19621 tggcttcttt taaatcagtt accgttaact gatattttgg cattctcaca gacttcattg
    19681 gattttcact tatcaaattc tttctttgac aatatttaaa aatcatattt agaacttgaa
    19741 taagtgattg taaactattt ttggcatagc caacatttgt tccattaacg ccacattcct
    19801 ttaaggtaaa aagcaattct tgaaggcact ctggtgtcaa atcagacaac tttttatcac
    19861 caatccatcg aacaataatt tttaattgtt cctctcgctt atcataggtt atttcctttt
    19921 gttcatttaa cgaacggtaa tattggaacc attgctcacc tacaacacta aacttgtcat
    19981 tgcttgcaac aatttttttc ttaagccttt gttgtttctc catttccaga taagcgagtt
    20041 gcgcctcacg ttttgaatta aagccacgtc ttgttgttcg atatgcttga aagtaccatg
    20101 tgatcctacc agtagaactg atttgcttct tgaatcgatc tgttttttcc atttattgat
    20161 tcctcctatt ttgaaggaac ccaacaagca gaaacccttt ggaaatcttt ctatctatgt
    20221 catcattgta ccgcatctta gaaagaaaat gtaacttata aagatcgaag tagtcgcaat
    20281 ttttctcgat agtcattcga atcactcttt ctcatccatt ctaaaacatc atcacgaaaa
    20341 tagtgtcctc gaatcccgta ccaacaggga ggaaactcac caagttttac taaacggttc
    20401 agtgtactat cacttatctg aaatagtcgc tttaaatcag tatttgttaa taccagaggt
    20461 aaatgactga ctgcaagcaa tttttccgtg taatcttcca gttttttttg taaaattgaa
    20521 tagaactcta tttccagaga attaacattt gttttaactt ccaactccat cacccttctc
    20581 tctcaaaatc aaaacgacca ccacggtacc ccttaaaatt gtaaaaaaac actccagtac
    20641 tccatcaaaa cggactagaa aagaggaatt tttaatatga aacaaggaat tgcagacata
    20701 aaaataatta aagaaattct tgaaaaatct acggctaatg caatagcatt cggaacagga
    20761 atcaacttga gtacagtaaa aaaacttaaa agtggagaga gagctgaaga aaaattaaat
    20821 cttgccgatg ctattaaaat cacagaattt ggcatgaaaa atatgccaac caaaatcgaa
    20881 atatggaaat agtagttttt ctgatctatc agctgttttg ccaaagcaca taggaatctc
    20941 acctcccccc agtaatggcg ggccgatttg gttacggaag tatcattatc gtagtcgcga
    21001 ttctcatatt aaaatttgtc gcttaaaagc taaccttgtc atggcgtaac tagagaatca
    21061 aaatctacat gtactgatag atttgctatt catttgtcaa agagcgctag gctattttca
    21121 gcctatggaa atcctttatt ttgacccgat aaaaaacttc catagaatga aaaggaaagg
    21181 gaaaaaatac tttaacgagt tgtatcaaat tcaagtatct tctcaatcaa ttttgtttcc
    21241 agttgtctac gcatatattc atctacatac ataaccattc ctttttcttc ttcataaact
    21301 ggtttcatgg ctaaccgtga aatataattt tgatattttg acagaacgat ggacatggca
    21361 attgaatcac cagaagctgc ggctttgatt gtcgttagtg gtatgaatgg tatatatcgt
    21421 ttattttgct tcatatttga cacccaaata ctttcctagc tcttccaaag ccatttgtcg
    21481 ttggtaccaa acactgccga gacttttccc aataatatcg ccaatttcac gatcattaag
    21541 accgatgaag aaggacataa aaatgatttc ttgtctttga ctaggtaatg ctgcaatcgc
    21601 atttgccaac tcctcattgg aaataaagat tttcatacca aggacaataa aagtaccact
    21661 actattcaaa tttggttcca aataatagaa ctctgtacct aacccttttt gttccaatat
    21721 ttccattgat atttgccgtt ggcgaaatct agcgtacttc ttttgaatat ttaacgcctc
    21781 attgcagact actttcttac aaaaactgtc aaacgtatgt tgaatgtggg tttcctctga
    21841 gtcgcccata atattctccc cctcaaaaaa ttcattcaac tcgttttact tctatccctt
    21901 tccattatta ctacaatcta gga
//
LOCUS       DAZKOW010000047        21362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000047 DAZKOW010000000
VERSION     DAZKOW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.047"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            62..1018
                     /locus_tag="ACG912_001624"
     CDS             62..1018
                     /locus_tag="ACG912_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0512947.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1091..1207
                     /locus_tag="ACG912_001625"
     CDS             1091..1207
                     /locus_tag="ACG912_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFQ0512948.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1262..2944)
                     /gene="recN"
                     /locus_tag="ACG912_001626"
     CDS             complement(1262..2944)
                     /gene="recN"
                     /locus_tag="ACG912_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFQ0512949.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2962..3411)
                     /locus_tag="ACG912_001627"
     CDS             complement(2962..3411)
                     /locus_tag="ACG912_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0512950.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3518..4336)
                     /locus_tag="ACG912_001628"
     CDS             complement(3518..4336)
                     /locus_tag="ACG912_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFQ0512951.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4345..5235)
                     /locus_tag="ACG912_001629"
     CDS             complement(4345..5235)
                     /locus_tag="ACG912_001629"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0512952.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5237..5461)
                     /locus_tag="ACG912_001630"
     CDS             complement(5237..5461)
                     /locus_tag="ACG912_001630"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFQ0512953.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5466..6803)
                     /gene="xseA"
                     /locus_tag="ACG912_001631"
     CDS             complement(5466..6803)
                     /gene="xseA"
                     /locus_tag="ACG912_001631"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFQ0512954.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6804..7658)
                     /locus_tag="ACG912_001632"
     CDS             complement(6804..7658)
                     /locus_tag="ACG912_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFQ0512955.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7755..8204)
                     /gene="nusB"
                     /locus_tag="ACG912_001633"
     CDS             complement(7755..8204)
                     /gene="nusB"
                     /locus_tag="ACG912_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFQ0512956.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8197..8622)
                     /locus_tag="ACG912_001634"
     CDS             complement(8197..8622)
                     /locus_tag="ACG912_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0512957.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8868..9932)
                     /locus_tag="ACG912_001635"
     CDS             complement(8868..9932)
                     /locus_tag="ACG912_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFQ0512958.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10104..10349)
                     /locus_tag="ACG912_001636"
     CDS             complement(10104..10349)
                     /locus_tag="ACG912_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0512959.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(10488..10862)
                     /locus_tag="ACG912_001637"
     CDS             complement(10488..10862)
                     /locus_tag="ACG912_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFQ0512960.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(10945..11859)
                     /locus_tag="ACG912_001638"
     CDS             complement(10945..11859)
                     /locus_tag="ACG912_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0512961.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(11880..12683)
                     /locus_tag="ACG912_001639"
     CDS             complement(11880..12683)
                     /locus_tag="ACG912_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFQ0512962.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(12716..13705)
                     /locus_tag="ACG912_001640"
     CDS             complement(12716..13705)
                     /locus_tag="ACG912_001640"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0512963.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(13808..14299)
                     /locus_tag="ACG912_001641"
     CDS             complement(13808..14299)
                     /locus_tag="ACG912_001641"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFQ0512964.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(14491..17298)
                     /locus_tag="ACG912_001642"
     CDS             complement(14491..17298)
                     /locus_tag="ACG912_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0512965.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(17732..18025)
                     /gene="rpmA"
                     /locus_tag="ACG912_001643"
     CDS             complement(17732..18025)
                     /gene="rpmA"
                     /locus_tag="ACG912_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFQ0512966.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18038..18382)
                     /locus_tag="ACG912_001644"
     CDS             complement(18038..18382)
                     /locus_tag="ACG912_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFQ0512967.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18409..18717)
                     /gene="rplU"
                     /locus_tag="ACG912_001645"
     CDS             complement(18409..18717)
                     /gene="rplU"
                     /locus_tag="ACG912_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFQ0512968.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(18737..18813)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            18999..19640
                     /locus_tag="ACG912_001646"
     CDS             18999..19640
                     /locus_tag="ACG912_001646"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFQ0512969.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(19702..21333)
                     /locus_tag="ACG912_001647"
     CDS             complement(19702..21333)
                     /locus_tag="ACG912_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFQ0512970.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
BASE COUNT         6150 a         4738 c         3577 g         6897 t
ORIGIN      
        1 ttccatgtat tccaattccc tatccgttat aataaaagaa acaggcaaca ggaggaaaat
       61 catgatcaaa tattttacat tagataatga gaaactgcgt aataccggaa aggaaactga
      121 ccaaacgatc tggtattgtg tagaacgtcc taatcaagaa gagattcatc agctgacaca
      181 tcaatttgaa attccgtcag attacatcac ttccatattg gacgatgcag aaaattcccg
      241 tacagaagaa ttcaatcaat caaagttgac aaagccagcg ttattattgt tacaatatcc
      301 ttttgctaaa acaagtccta gcggttattt tcaagtggat acatacccgc tctctattat
      361 tttgacgccc aaaaagaagc ttatcacaat tacgaatcat gaaccactgt ttttaaagaa
      421 agtctttagc cagacctttc caaaaagtga tatttcgcct aatctcaata tcttctttca
      481 attcatttgg caggtcacac tctcttacaa cagttattta gctacgcttc aatctcaatg
      541 cgatactttg gaagccaaac ttcaagtatc cactgaaaac aaacaacttt atcaaatcat
      601 ggatatccaa aaaagcctcg tttatttcag agaagcgact tctgctaatt tagatgcatt
      661 gaaagaacta gcgcaaaata aacttttcca agaaaatcat gctttgaaaa atcatttacg
      721 tgatgtattg gtggaaacag agcaggcaat gactacttct cgcattcaat taaaactagt
      781 agaacaaatg aatcagactt tttcagcaat cgtatcgaat aatctgaata atattatgaa
      841 aattttaact tctttaacga ttattttaac tataccgaca atcgtcggca gtatctatgg
      901 aatgaacatt aagttaccta ttgctgaaag ggatgatgcc tttatatggc tagttctatt
      961 aacattattt atttgtatcg taactaccta ttatttaaaa aaagggaagt ttttataaaa
     1021 aagccctctt gtggaaatgg aaaaaagatt gtacacttga atcaactaat taatgacgag
     1081 gttgaaaaca atgcgtgaaa aaaaacaaac aagtactttt gctaaagtaa caaaatttgt
     1141 catttggaca atgctgattt taacgattgg atctgttgta ttgactgctg taatgagtgt
     1201 catgtaaaaa taaaatatag ctatcagctg ctgaaaagga cctgaaccac tgcgaggtgg
     1261 ctcaggtcct ttttgcgagt ttcaatagtt cttttgcatg ttcgagtgtg agttttgtaa
     1321 tctcacttcc agccagcata cgtgcaattt cttcttctct ttcttttgtt ttcaactctg
     1381 aaacagaagt ttgcgtacgt ccatcaatca ctgctttcac aatataatat tgatagtcgg
     1441 cgactgcagc aacttgaggc agatgagtga tgcacaaaac ttgagagtat ttcgatattt
     1501 gagaaatttt atctgcgatg gcctgtgcaa ctcttccgct cacacctgta tccacttcat
     1561 caaaaataat acttgtcaca ccttgctcag aagaaaaaat agttttcaaa gcaagcaaca
     1621 tccgagacag ttcgccacca gaagcgactt tcaccaaagg cttcagtggc tctcctggat
     1681 tggtcgtaat gtaaaattcg atttggtcaa atccatcatg attcaattgt ctattttctt
     1741 cttttaagaa acgtacctca aactcagtgt tttccatata cagacttttc aattcatgca
     1801 gaatggattt ttccaattct tttgcgattt ttttacgcgc ttcatgaaga tctgcggctt
     1861 gttgctgaag tagaagctct ttttgctcta gttcctcttt cattttgtcc aattgacctt
     1921 ctgaaaaatc agaagaagct agctcttcgg tgatttcgtc atagtaagcc aatattgact
     1981 caatggattc gccgtattta cgtttcaact gtcggattaa ttctaaacgt tgcgtaactt
     2041 cttctaaacg actctcatct aactccaata aatcaatctg acgactcata tcgccgattg
     2101 catcttgcag caaatagtaa gcactttgga tattttcata aattgcttca tactccacat
     2161 ctagatgggc gatcccttgc agttctgaaa cggccaagcc aacaccatct acactgtttt
     2221 gttcattatt cgtcagtgct ccgtaacctg cagctaatcc atcagcaatt ttttgataat
     2281 tgctaagttt ttctctttct tcttttaatt tctcttcttc tccgacttgc aaatcagcag
     2341 cagctatttc ttcttgctgg aaatggagca tatctattct ctgaacatag gatttttcat
     2401 tttgctgcac ttttcgtacc cttttttcca gctcacgata agagatatat tcttgctgat
     2461 aactcttttt cttctgttgg aaagctacat ctccaaaacg atccaataaa gcaagatgtg
     2521 attcaggctg gagcagttcc tgatgttcgt tttgtccttg gatatctact aaataactgc
     2581 cgattcttct cagattagct aatgtgataa tatgaccatt cactcgacaa acattttttc
     2641 cagttaagga catatcccgt cgaacaatca gattgtcctc atctgtttcg atccctaatt
     2701 caaccatcag ttcagagaat ccttcttgtt ttggtagttc aaacaaccct tcaagcacac
     2761 atttttctgc tccttgacgt atataatcgc ttgaacctct tcctcctgct aacagaccta
     2821 aagcatcgat aataatggat ttacccgcac cagtctctcc ggtcagcgcg gtcattcctt
     2881 cgtgaaaaga aagccgcaat tccggaataa tcgcaaagtt agtaatactg atttcttgta
     2941 gcatcacatt cgctcctcaa cttaaaggta atgcaggaag tctttcttca ataaaactgc
     3001 ctggtcttca gttcgtgtaa tcatcaaaac attatcgtca tcattgataa tcgaaaataa
     3061 ttctttcttg taacgtttat caatcaaatt tgctgctgcc gaagcatttc ccggcaatgt
     3121 tttcaaaata acaaattttt ccatttggtc aactgccacg aaagcgtctt ttaatagttt
     3181 ttccaattta gtatttacat cttcgtttgt ttcagctggt atgctataac ggtagccgcc
     3241 atctgaagcc ggtaccttga tcagtttcag ttcctttata tctctggaaa tcgtagcttg
     3301 agtaacagat attcctttgc ttttcaggat ttcaacaaat tcttcctgct ttcggatatc
     3361 ttgctcactc aacaatcgag taatcaaacg atgccgatct ttttttctca ttacgtcttc
     3421 gctccttagc atattcttct taatcaatga tagcttattt ccttggtata ataaagaaga
     3481 atccgcatac ttctcacttg attttcacag ttacaattta tttatggtca aactgttcgt
     3541 gtgcatttga aaccacttct gccatattaa tagctgaatt gattgttccg ctttcgggta
     3601 ctttcctcaa gtgagccaag aactcgatat ttccttctcc ccccgtgatc ggagaataac
     3661 ttaattccag cacatcatat ccaccttgtg ccgcaaatga caaaatgtct tctaaaactt
     3721 tttgatgggt ctgagggtca cgaacaattc cgtttttccc tacagcttct ctgcctgctt
     3781 cgaattgggg tttgattaaa gcaatcactt ctccctcttc ttttaaaata gcatgtaatg
     3841 gaggcagcat cagttttagt gaaataaaag aaacatcgat aacagcaact tctggaattc
     3901 ctaaggtaaa gtcttcgggt tttgcataac gaaaattcac tctttccatt acgacgactc
     3961 taggatcttg tcttattttc caagctagct gattgtagcc aacgtctaaa gcataactca
     4021 tttttgcgcc attttgcaaa gctgcatcag taaatccgcc agtggatgca ccaatatcta
     4081 acaatgtttt tccttcaaca gatagatcaa atgctttgag tgctttttct aattttaatc
     4141 cccctcgaga aacataagga agttttttac ctttgactct taattcacta ctcactggaa
     4201 ttttttctcc cggtttatca aaccgctcgt ttttttcgtt gtaaatcaaa ccagccatca
     4261 ctgaacgttt ggcttgttct cttgtttcaa ataatccttg tttgaccgct aatacatcta
     4321 cgcgttcttt ttccattctt tccctcattt ctccacatca aattgcttga tcatctggcg
     4381 catcagcccg ccatcaaaag aaaaaacgtc ctcttctaat ttgtctatga tcttgttggc
     4441 ggataacagc tcaatctcca acgcctcacg tgtcttttct aggcctaata aacgcgggta
     4501 agtatttttt tccatacgtt catctttacc agctgttttc cctaatgttt tcgttgtacc
     4561 aatcacatcg agtagatcat ctcttatctg aaaagctaaa cctaaatgtt cggctaatcg
     4621 ttgaagtagc aataagattt cttcaggttg cttcgctaat atgccaccag ccagcaaggc
     4681 ataacgaatc aaatgccctg tctttctttc atgaataaat gctaattctt ctaaagaaag
     4741 ctttttcgat tcgccctcga tatctgcagc ttgcccagca accatacctt gtgatcccgc
     4801 acaaacagct aattgctgaa gcagcagaag cttaggagaa ttacctaagt gtgccatact
     4861 gattagctgg aatgctcctg taagcaaacc atctccggcc aatatagcta atgcttcgcc
     4921 gaatacttta tgattggtag gttttcctct acgaagatca tcattgtcca tcgcaggcaa
     4981 atcatcgtgg atcaaggaat acgtatgcac catttctaaa gcagctgcta cttgatagac
     5041 gggaacatca ataggctcat caaaggaagc aacggccgtt aaaagtaata agggacgtat
     5101 tcttttcccg cctgctttca ctgaataact catcgcatca tagagtcctc tatccgttgt
     5161 atgctcatct aaaaaagaca agatttcttt ttctacagca ggtagatgaa tagctgaaaa
     5221 gtcagttaat ctcatctcag ctttcctcgt ttccgtcaaa atcaacttct tcatttgatt
     5281 ctgtcatcat tttcgtcaag gtctcttctg ctttagctaa tgtatcttgg caatgtttgc
     5341 ttaattccat tcctcgttta aacgaatcta gagctgattc taatggaacg tctccttgtt
     5401 ctaactgcat gacaattttt tctaattctt gcagtgactc ttcaaaagtt ggattttcca
     5461 tatttttatt ctcctttgtc tatctcttgt acttgtcctt taacgattcc atcttgataa
     5521 tggatcgtca gcaaatcccc ttgatgaatc tcggtgacac tttttactac ttgatcattt
     5581 aacgtcgtat agctgtagcc tcttcccatg attttcaatg gactcaacaa atcaagagat
     5641 tgaacagctt gagcaaaacg ctgctgctgt tgtttcatat attgttggat tttctcttct
     5701 aagcgttttt ctagataagc agtttcttgg cgagctgctt taactcgata aagcggagcc
     5761 acttgttcca attggtggct taatgcgatc gtttgttttt ctttcgcatg ataaatttgc
     5821 tgtgtagctt gatataatcg ctgtttcaac tggtctaatt tgatggtctg accttcatac
     5881 aatctttcag gctgccgaaa tacataggaa cgtcgagcac gctcgaaccg ttcttgtttg
     5941 cgctggatct ggcgtacaaa cgcctgctgc aatctagctt gccgttcttg aatgcgcatc
     6001 aactcttctg acaaaacagg aacagctaat tctgcagctg ctgttggagt ggcagcccga
     6061 acatctgcta ctaaatcagc gatcgtggta tctgtttcat gtcctacaga tgaaatcacc
     6121 ggcgtattag cttgataaat cgcccgagca acccgctctt cattaaacgg ccataaatcc
     6181 tcaatcgaac cacccccacg accgatgatc attgtatcga aattgcccat ctcttctact
     6241 ctctggatat tcctgacaac attgtccgct gcttggtttc cttgtacaac tgttgggaac
     6301 aaaaccaatt ggacaattgg atatcttctt ttcaccgtcg tgatgatatc gcggataacc
     6361 gctccgcttg gactggtaag gaccgcgatt cgctttggga aacgtggcag cattttttta
     6421 ggcccttgaa aaaggccttc cttcatcagc ttttcttttc gttcttccaa ttcttgatat
     6481 aaagcaccta ttccatcagg ctccatgtgc tctacataga tttgataaga acctcccgct
     6541 tcatataatg aaatacgccc aacgacaagg actttcattc cttcttttgg ctggaatcgt
     6601 aatttttgga atgctccttt gaacataata gcagatattt ttgcatggtc atcttttaag
     6661 ctgaaatatt gatgggcatt ggcacgtaaa cggaaattcg atatttcgcc tgttaaatat
     6721 acgcgttcta aataaggatc ggcatcaaat tttcttttca aatacttcgt taatgtagtg
     6781 acagttaaat attcttgtgc catctattca cctgctccaa aagcttcggc agattttacg
     6841 gtttgataca tcagcatcgt gatagtcatt ggccctacac cctttggcac aggagtgatc
     6901 cagcctgcaa taggctccac ttcatcaaat ttcacatcac cgatcagctt accgttagca
     6961 tcacgattca tccctacatc tatcactaca gcacctggtt ttacgaattc ttttgtgaca
     7021 aaatgtccac gtcctattgc tacgactaaa atatctgctt cttgtgccag ttcagccaga
     7081 ttttcggttt tagaatgagc aacagtaaca gttgcatctg ccatcaagag tagctgagcc
     7141 atcggtttac cgacgatatt gcttcgtccg ataactactg ctcgtttacc cgccggatcg
     7201 atagaataag cttcaaacat tttcatgatg ccgtatggag tacaaggaat cttgtccggt
     7261 ttacccgcaa ggagtctgcc taaattcatc ggatgaaaac cgtctacgtc tttttcaggg
     7321 cggatagcta acaggatctt ttcttcatct atatgctttg gcaatggtaa ttggaccaat
     7381 ataccatgaa aactaggatc ttgattgtat ttttcaattt ctgcaagtag ttcgtcttca
     7441 gaaatcgtct caggataacg ctctaccaat gaatggattc cgatttcttt tgctacacgt
     7501 tctttatttc ttacatagat ttggctggct ggattttccc ctaccaataa aacgactaat
     7561 ccgggatgga taccattatc tttcaatttt tggatttttt cagcaatctc tgcctgcatt
     7621 ttttcagcta attcttttcc gttaatcaat tctgccatct aaccactccc agatattttt
     7681 tgtgttttca ttctaccata gaattttccc gatttataac aggtttatga tgattcatag
     7741 aaaaaagcta ggacttacgc cccagcttct aattctttca atacattgga taaaacaccg
     7801 ttgacgaatt tacgtgaacg gtcatcacta aatgtttttg ccaactcgat tgcttcattt
     7861 aaagcaacag ccgctggtac atctgtcaca taagtcattt caaaaatcgc gatacgcaag
     7921 atcaccaaat ccatttttga gatccgattg atgtgccaat tgttttttaa atgtttttca
     7981 atcactttat ccaattcatc tttttttgca cagacaccgc caactagcgt atcaagataa
     8041 acaggaacaa actctgattc atcttcgttg atcatgtcgc ggtggtctaa ttcgattgcg
     8101 ttgaaaatcg catcttcttt tgttaggtct gcattgaagt ctaatggaaa taaagcctgc
     8161 aatgccattt cccgaatctc gtgtcttgat aattctttat tcattctctt cctcttcgtc
     8221 ttcaaaaagt tcttcgattg ttggttgttc taatttttca ggaacaacag ctactacatg
     8281 gatattgact tcagataaat cgatatccgt catgaaaagg acttgctggc tgacccgttc
     8341 ctgcatttcc attgctactt taggaactga aacaccatat tctagataac agtaaagatc
     8401 tactttcaag ccatcttcct cgtttgctaa gtaaacacct tttccgtgag ctgcacgtcc
     8461 taataattca gtgacattgc tggcaaaagt tccgcgcatt ccgtatacgc cttctacttt
     8521 agaagctgca atcccgataa ttacttcgat gacttcagga gcgatcacga tctctcctaa
     8581 atcttggttt gcgtttaaaa tcaaattttt ttcatctgcc atttatttat tcctcccttt
     8641 gcctaagcac gagatacatc cgaattctct atgatgcggc atagtgatca cgtggtaaga
     8701 aaactgccgc cgatcagctg caaactgcta tgaattggag aaaacaccca actttattat
     8761 ctcttacagc ttaaaagttc ttctatagtg tatcattaaa tcgagtgatt ttctataaca
     8821 aagtctgtga acactgattt ttacgcttac ttcttctatt gatgaaacta taagatgatc
     8881 aattcttttg gtgaacgagt caacacacga tttccatctt ctgtgatcaa caagtcatcc
     8941 tcaatacgta ctccaccgat tcctggtaag tagatccctg gttcatcagt gatcacattt
     9001 cctggtacaa attgcttttc tgcacgagaa gaaacgtttg gtccttcgtg gatctctaaa
     9061 ccgatcccat gtcctgtaga atgtccaaat gcttccccgt aaccatgttt tgaaatataa
     9121 tctctagcaa ctgcatctaa ttggacacca gtcattcctg gttttgcagc atcaataact
     9181 gctaattgag catctaagac tatttgataa atttctttta atttttcccc aggatcacca
     9241 atagcgaatg tacgtgtcat atcggaaacg tacccttcgt aatagcaacc aaagtccaat
     9301 gtgatcaagt caccttgttc gatgattttt ttgcttgcta caccatgcgg catagccgaa
     9361 cgcaatccgc tagcaacgat cgtatcaaaa gaaacacctg aagcgccaag agaacgcata
     9421 taaaagtcta gttgatttgc gacttcaatc tcagtcatgc ccggttggat cattttcagg
     9481 atgtgatcat atgccatatc cgcaatatga catgcttttt cgataatagc gatttcttct
     9541 tcatctttca cttctctgag ttcttcgatc atctgtgaaa caggtactaa ttccgcatcg
     9601 atgatttctt ctaaaaccga atattctaaa aatgagactg ttgtttcctc aaaagctaat
     9661 gagtgcagtt cttctttgcg tactaagttt gctacctcat caaaaatagg tccgacattt
     9721 ttaataattt caaacccttg agcctgcgct gccgcctgct cggtataacg aaaatcagta
     9781 ataaaaaatg ctttttccaa agtaataaca gctaatcctg tcgtacctgt aaagttcgtt
     9841 aagtaacgaa gattgtacgg actggtaacc aaaaatgaat ccaaattttc ttcttgcatt
     9901 tttttgcgca atttttctac acgtagcatc ataatgaaac tccctctttc tttgcacgct
     9961 tttttccatt atagcaaact tttcttcgta cacgacagtt aaataaaaat ttccggaaaa
    10021 cttcttttta ttttaaataa ataaagccaa aaaggagtca cagtctcgct gcaactcctt
    10081 ttagtttact attttgtaaa aacttatgat cgttttccaa agaaaaatga gacaactgct
    10141 accaaaatca ctgcgcctaa aatagatgga ataagtgcca tacctgcagc ttttggtccc
    10201 cattcgccga aaatcgcttg tccaatactt gaaccgacta gtccagcaat gatattcgtt
    10261 acacagccca tcgaatttcc tttactggtg atcgcacctg caattgctcc gatcacggct
    10321 ccaacaatca atgtccataa ccaactcata atataacctc cttctttgct aatcaatttt
    10381 agcatatggg atttaaaggg gacaaataat tggttttgct atttcatatt ttcttctgca
    10441 aagtaaaaaa ataaagaaaa cctcctctta gaagttttct ttatttttta cttgcgttta
    10501 aacatactac gaataccacg tttcactaca tcaaagaaac tcaatgattg taccattcta
    10561 tcttcatcta catattctct gatcgcacga agcacttttt taggttcttt ggaagaaaat
    10621 gtgtatgtac catttttttt cgtttgaatg gcatatcgag gaatccattt tccctttagc
    10681 atgactgatg ctagtacata atccacttct tcccatggaa tttggatata tttgcgtcta
    10741 tctcgcgcat ggtaaaactc aaaaccatta ttacctatca tgatttgccc gtaatctgtc
    10801 agacccgtaa acgaagtagc atcaattacc aagtctactt ttgtattgat tgattgaacc
    10861 attttctccc tccagatatt tgtttatgaa cagtatacta tataaaaaaa gctcgggaca
    10921 acgcccgagc tatattttta tggattataa aagtccgatg acgtgtccaa caacaccgac
    10981 tacgaataaa ccaaggatga taacgattgg agaaaccttt ttcttaagca accacataca
    11041 taacaatgtt aatcctaatg cagctaaacc aggaatcaat tggtctaagt tgtcttgtaa
    11101 tgttgttacc ttttcaggag aaagggctaa gccattatta acttgttcaa atgcactctt
    11161 gattccttca ccacctgaag gcaatttatc ccattcgatg taagcacctt tatctaattt
    11221 tacagttgat acaactggta agaatttgat tgatacccat ctttgtacaa gggcagctaa
    11281 tacgaacatc cctaaaattg aagcaccttt tgttacgtct tgtaacaatc cgccagaaag
    11341 gtcatcagta attttagaac cagctttgta gccaaattct tgtgtatacc acatgaatgc
    11401 ccaacgaata atgttccaac ctaagaagaa gatgattggt ccaaggatat ttcctcccat
    11461 agctaatgaa gcacctagtg cacctagcat tggacgtact gtaaaccaga acactgggtc
    11521 accaacacca gctaaaggac ccatcatacc tactttaacc ccttgaatcg ctacatcatc
    11581 aacaggcgcg ccattcgcac gttcttcttc cagagccaat gtaaccccta aaattggaga
    11641 agcaatgtat gggtgtgtat taaagaattc caagtgacgt tttaatgctg cagaacgttc
    11701 ttcttttgtt ttgtataatt ttttgattgc tggaatcata gaaaatgccc agccaccgtt
    11761 ttgcatacgt tcgtagttcc atgaaccttg aatgaaggta gagcgccacg caacagctaa
    11821 acgatctttt tttgataatt tgattttttc ttctgccatt ttattttcct ccttcaagat
    11881 taatagtcat tcaaaatgtc gcctaaaggg tcaccagagt tgcttccgcc tccattattt
    11941 gatgaaccac ccattttaga aaggttcaag taaataagag ctaatgcaac acctaaagca
    12001 cctagagcaa tcaatgttaa ttgagaaatt gcagctacaa cgaaaccaat aacgaagaat
    12061 ggccatactt cttttgtagc catcatgttg attaccaaag cgtaacctac agctacgacc
    12121 attcccccac cgatagccat accatctgtt aaccatgctg gcattgattc aaggaatgat
    12181 tgcacagttt gtgctgggat aaatagcaac gctgctgctg ggaccgcgat acgaacccct
    12241 tgtaaacata cagcaatgat gtgccacatt tcaaccttac ggatgtttcc ttcttcagca
    12301 gccgcatcca tcaaatgaac gattggaaca gcgattgtac gtacgatcat tgttaagaac
    12361 aaccctgcaa cagccaaagg aacggcaatc gcgatcgcag aaggaacacc tttaacacct
    12421 tgtccaccta aaactaaaat aattgctgat gctactgatg ctaatgcagc atctggtgct
    12481 acagcggcac cgatatttgc ccaacctaaa gcgatcatct gaagcgtacc gccaagtacg
    12541 ataccagctt cgaggttacc ggtaactaaa ccgatcaatg tacatgccac tagtggttga
    12601 tgaaattgaa attcatcaag aataccttcc ataccagcta gaaaggcaac taatacgact
    12661 aaaattattg aaataataga catgataagc ctcctataaa ttttaatatt atttattacg
    12721 cttttgctaa ttcagctttt gcttttttca aaatatcatc catgttatcg cgtgaatcat
    12781 taggtacttt acgtacatcg aatttcacac ctttttgttc aagcttctca aacgcttcaa
    12841 cgtcttcttg tcccatagac aatactttac ttacaacgac tttaccgacc gaatgcgcca
    12901 ttgacccaac atttaattct ttgatgtcta cgccaccttc aacagctgtc aatacgtctt
    12961 caggattttc aaataaaagc aatgctttgg tatttccaaa acgagggtct tttgctactt
    13021 cgatcatttt gctaatagga ataacatttg cttttacacc tggaggagca gcttgttcga
    13081 ttagtctctt acgcagatca tcctttgata ctgcatctga aacaacgata atacggtttg
    13141 gttgcactgc ttttgtccaa gctgtcgcaa cttgtccatg taacagacgt gagtcaacac
    13201 gtgctaatac atatttgatt tttccgtctc cgaccacagt tccttctgga agtgctcctt
    13261 taggttgtgc gtcttcaact gctgctttcg gtgcttctgc cggttctaat tcttccggtt
    13321 ttacttttac accgtctttt gctgtttcaa tgatgtgtgc tgcaatttca tgagcagatt
    13381 ccattgaaaa gcgtgatgcg taggcttcaa tcaacattgg taaattcaaa ccgctgacga
    13441 ttgcccattt atctttgtgc tcttcgaata atgtgtttga ttgattgaac ggtgttccgc
    13501 cccataaatc aactaagaat aacacttcgt cttgattatc aaaagaagca accgcttctt
    13561 ccaatttggc tctcaagtca tctggacctt cacttggttt caaaatcacg gctttgacgt
    13621 tttcttgttc gccaaagatc attgaaccgg attgtaagat tccttcagca aattgtccat
    13681 gacttgctag gataatccct accatattta tacctcctat ttacttttgt tgtaacaaat
    13741 tcaaaaatat taataattgc taaccattac aacagaatta tttgaccttt tccaacaacc
    13801 attcttgcta cacctttttg attttcgcta gtagttccat caaaaggatt ttcctgtcag
    13861 caggtacttt tcgaatctct aactccactt cttgactgtt caagtaacga aaggaatcaa
    13921 tatcttgttg atttactgaa acaaaattgg taatcattga tttgccttca gtaaaactca
    13981 taccgccgat attcaacgac ttcaactgta caccgcagcg gacaaccgct tcaacatctt
    14041 ggggattggt gaataaaagc atcacttttg ctcgtgataa taatccacta tgatagattt
    14101 gaatcatttt ctgcacggtt attacatttg ctgtgatccc cggcggtgca gcttctttta
    14161 gtagaaactt tctcaattta tcttgagcga ctacatcgct gatcacaatg atacgttcaa
    14221 cacctgtttg cttcgaccaa actgttgcga cttgcccgtg gattaaacga tcatctatac
    14281 gtgccaatcg aatatccatc tgcaaaactc cttcttcctc actacttcac agaaacccct
    14341 atcatttctg tgtgaacaac cacctcctag cacacactac tataagcaaa aagcgtgcca
    14401 acttttttta cgatacacta attgaattta aaggattctt aaccaataca caaaaacgat
    14461 acactataaa agtgtatcgt ttttgtgtat tcatggcaca gtgtattctt ttttatctaa
    14521 ctgattatgg actagacgta gcagataata catttcttct atcggcactt ttacttttat
    14581 ctgatcttcc aacagaacaa ctgaggctgc caattggcta taaagaggat cattcgttgt
    14641 atttgtcaat tcgttttctt ccacagtcaa tggttcattc cgaataatac gttcgatcat
    14701 tcctgctgtg tgcatcacta ggttgatttt gaatccatat tcttcatcgc cgattcccgt
    14761 cgtctgagag atttgtgtag aaaattgcca cattggatcg attaacttac taccattaat
    14821 aaagatgaag ttatcagaga taaactcaac acacgtattt ttagctgact cttcatctag
    14881 ggagatgaat tcagtttcct ccaattctga ttccatcagc aactgatcca aaatgacttc
    14941 gatattttga tcaataaaac gttctaaagg aataaatggg gcttggatat tcaaatcagt
    15001 aatcccagta gtagctatga tctgatattc tttttggata actggcagtt cttctttcac
    15061 ttccacaatc gacaaagcta gaacttctaa ttctgtttct tgacgcttgt ttaccgctcg
    15121 ttcgatcaat tctttgattc tttgtgctgt accttcgcca gatgcgcaga tagccaaaac
    15181 agcttttttc ttatgaatag gttttgcttc ctgaacagat tctacagaag atatttctgc
    15241 gtaacctcga aaatttttca atgattcatg caacatatct agatctgtcc ctaatactga
    15301 gacctttcgt acagtttcca aaactagaga agttgttacc atatcaacag tacgtactgg
    15361 aattccagtg tctctttgaa tttgcgaatt aaacgacgca agggacccca tatctactaa
    15421 gagtatgacc ccgcttcctt catctacttc ttgtacattg cgttcaattc ggtccaatgc
    15481 tgttttcgga tccatatcca aaggcatgtc tactgctcgt acgttctctg catctaacaa
    15541 ctgttggaca acttggacca tactgcttgc tgtgctgttt ccgtgagctg caacgactac
    15601 accgattctg ccgcttgctt gatttgcccg taacgagacg agtagaacgg tcaagtaata
    15661 gtcttcactt tctggaatct ccacttgaaa atacgtttcg atataactac gcacttcttt
    15721 cgcaacagca tattcatctg gaaaatcaaa agccatctca cggatgttat cattcgtatg
    15781 ccgttcttct cctaagttga tttttttcaa aaaagaactg atatgcaagc tcatagcata
    15841 aataaagttt tgctgaaact catatggtaa agatcttttg accattacat aaatctgatt
    15901 ggtcacttct atgattttcg gatctacaaa ttctgctaat ttgctgtctg cattaaaaga
    15961 aaaaccatgg tctttataga aagacttcag atggatgttg atatctgtag aaatgaaatg
    16021 attgattgct tcttgatcca aaccatccga cttcagtaaa gctgctttat ctccgatgat
    16081 atcatataga ttataaggta actcatagga gtccgattgg attttcatga tcgtatcatt
    16141 tggtgctaca gttatctttg gctccaatat tttagaaagc tctgccattg cactgcggtt
    16201 gcttgctaat tgaatcaatc cgctacgaat cccttctgtc aaatcatcga ttgtgatcga
    16261 gatttcgggt gattgcatat gatttagaaa tcctcgtgca catactaact ggatatttga
    16321 cttcaattgt cctatatttc cataggtcac actgccgatc aatgcttttg ctacatcttc
    16381 tgtcaaagaa atctttcgct gcgtgcggtt tgcttcgtgg gcaatcatga tttttactaa
    16441 atcgactttc tcatttgcag gacgtttttc aaaagccgga agtttgatat tgattgggat
    16501 acgccgaaca aaagtttcta atagagaaga acctgggtct tctgtggtcg cacctacgat
    16561 ccgtacattt gcttcatggg atttcgttgt ttccccaaga cgactgtaag ttccatgatc
    16621 cataaagtaa aagatcattt cttgaccttc aggcggcaac cggtgaatct catccaaaaa
    16681 cagcataccg ccatccgctt gatcgataat ccccattttt tcttcatctg cgcctgtaaa
    16741 agcacctttt gcgtacccaa ataaatgact catcaaaagc tcagggttat tggcataatc
    16801 agcacaattg aaaacaatca gttccgtttc ctcatctatt acatgatgcg cttttgcaaa
    16861 atgatacatc gcgtgtgcaa aataagtctt tcctgatccg gtaggtcctg taatcaaaca
    16921 atttaatcct cttggcggat acaaaatcgc agcttttgcc tgttccactg aatttttcat
    16981 gctgccgtta gctccaatga tttttgcaaa gatatcctta gtatctattt ggagtgtatt
    17041 tttttcttca gagaccggcg tttctttgta actgactaca tgttttgtaa ctggctttac
    17101 gtgcgtaacg agtgtttgat atatgtaacg tactggtcgt ccgtctgtct ttatcagttt
    17161 tccttcgcgc actagctgat tcagatcttt actgctattt gttcgctgaa tccctaacac
    17221 ttcagcaatt tcctgtgttg ttactccttg atcatattct gcctttgtta aatgagcggt
    17281 tttttctttg acatacatat aaattcggtc aatgcgtttt gccaatggta tcgctccttt
    17341 tctatagact attgtactta ggcttcaaaa cataatacta tgtatttatt tctgtttcgt
    17401 caactattta cctgattttg ttttgttgat aacacgcgga tagatagaaa tgttcaattt
    17461 aaaagaacga aaaatcccga tacgattttt ataatggaaa caagaaaaag aacactgttt
    17521 accatataat tatttcactg atagcaaaca atatagcttc catcaattga aaacgatagt
    17581 tatcaaaaca tcctgattcc aaattggcaa aaaaatatta agtgtaaaaa gacaaagtaa
    17641 acttcgtagc ctccactaca acttttattt ttataaacaa aaaaggctgt gacaaaagtc
    17701 ttgtcacaac ctctttaatc ataagattca attaagcttc ttgagctact ggataaacag
    17761 acacttgttt tttgttacgg cctttacgtt cgaaacgtac tacgccatca acttttgcaa
    17821 ataaagtgtc gtctccaccg atacccacgt ttacacctgg atagattttt gtaccgcgtt
    17881 gacggtaaag aattgatcca cctgtaactg tttgtccatc agcacgttta gcacctagac
    17941 gttttgattc tgagtcacgg ccgttagatg tagaaccgcc accttttttg tgggcaaata
    18001 attgtagatt catagataac aacatggtct gcacctccta tttatcatta attgttttaa
    18061 tttggatata ttctgagttt tcagcttcta tcgattgtaa tcctaataaa agattttcta
    18121 atagaatctc agcaatattg ttttgttctt gtgtcattcc tgaagtgact tcggtataaa
    18181 gataaccgcc ttcttcttca ttcatttcaa caatcggctc aaaaccggct aatgaggcaa
    18241 tcccgttgac agtgctgatc gctaacgcag aaacacccgc acaaacgata tcacttccat
    18301 aaggaccagc atcagcatgc ccggtcaaag tgaatgaaac gatctgacct gcgtcatttc
    18361 gcttaaatgt tcctttgatc atatataggc cttctttctc aaacgctatt aagcgttgat
    18421 cgcgtcgata accacttttg tatatggttg acgatgacct tgtttacggt gtgaatgttt
    18481 tttaggtttg tatttgaaag taacgacttt cttttgtttg ccgtgttttt caacagtacc
    18541 ttcaacagtt gcacctgcaa cagttggagc gcctactttt gtagattctc cgcctactaa
    18601 gataacttcg tcaaaaacaa ctttttctcc tgcttcaacg tcaagttttt caacatagat
    18661 tgcttgtccc acttcaactt ttacttgttt accaccagtt ttaataattg cgtacatgct
    18721 ataaagcacc tccttctgat atacttagac tcgccatccg aagtgacagc atatgctgtg
    18781 cttattactt gttctgagcg gttgtagctg tggagtccac aattacaaca gtactagctt
    18841 accagaacag caaccgttag tcaactaaat ttaaaatcaa aaagtaaaac aaaaattcaa
    18901 gcaatacttc tgcttttcta tcctcacagt tctaccaaat aaaatagtcg cattgtgttt
    18961 cttttattct tataataaaa gaaaaaggag gagcataaat ggaataccca aaagaactga
    19021 tacaagaagc ttctttaaga atgaacggcc gtcctgtaga aggatttatc gctgggcaag
    19081 gacctcttca tcctaaactg atgctcgtgg gagaagcccc agggaaaaca gagattgata
    19141 cgcatgttcc ttttagcggt caagctggaa aagaattgat gcaggcactt tcttcaactg
    19201 gcttaacacg agaagaggtg tatatcacca gtgctgtaag gagccgcccg tatcgcgtga
    19261 ctcatcggat caataaacgg acacaacagt cagaaactgt ttatccaaat cggacaccta
    19321 ctcgttccga agtatttgcg catgcaccaa tattggatta cgaactgatg aaggtccagc
    19381 caaaattaat tgcgacactt ggaaacattg gtcttcaacg tttacttggc aaagaatata
    19441 cggttaccaa aaatcatggt caactctata cagggcctgt cttacaatta acagaacaaa
    19501 aggatgccta cgaatggtca gaaaaaaact atcgaattct tccacttttt catccagcag
    19561 ctattttcta caatcgctcc cttgtacctg ttatccatca agactggcaa gtactagctt
    19621 cacttttaca gcaatattaa aaagaacaga caagaccata aaatcttcag tttacagtct
    19681 tgtctgttct ttttcattgt tttaatggct aattgcagta tatttcgcat gtaaagctgt
    19741 caacgcatga gcaactgttt gatgttgatg cgtgaatgta tgttcccaca agtgagaaag
    19801 ttgtttttct acttctggct gcaaggaagt atagataatc gtttgtcctc tttgctctgc
    19861 tttttcttga aattcatcaa aaactgcacg tgccgtcaaa tcaatacttg gtacgcctcg
    19921 catggagaaa atgacaccat cgtgatgatt tagttcttcc aatttatttt tgagtttttc
    19981 tgaagacata aaaaacagtg gaccactaat atacactact gtccagttat tcagcttttc
    20041 cgtttctggc agccccattc tttgccaatc gatcgtttct aatgaaatcg tgatagcagc
    20101 gcttttgaca acaaaaaata cacagccgcc gatcaccccg atcacgatag caatacttaa
    20161 atcaaagata actgtacaaa tcatcgtaag gataaacaat agaactgctg accaatattt
    20221 attagtaaat agctccttga tcgtttccca ttcattcata cgccaagccg tcacaatcaa
    20281 aacacctgca agtgctggca ttggaatctg tgacatcact ggcgcaaaaa caagcatgga
    20341 gagcagtaag acaactgcat gtatcatacc agctaatcta gtttgtgcac cagatttgat
    20401 ggccacgctt gtacgggcga tggccgcagt agcaggaatc ccgccaaaga aaggaagcaa
    20461 cagatttcca attccttgcg ccattaattc ttgattgcta tctaagggct tatttgccat
    20521 tcggccagcc gaagcaccac atagcaagct ttcaatcatt cctaataaag caatgctaat
    20581 agctggaact gccacttgtt gcaaggcaga aaagctaaaa tctcccatat tcaaacgatt
    20641 actactgatc aatgtttgtg gaatcttacc cacagttgct actggtaaat ggaacaatag
    20701 cataactgct gttgccaaaa tgatagccag cagcgaacta ggcattcgtt tcgcccattt
    20761 ttttggatag ataaacatcc ctaaaatgac tagccctccc atgatcaaag aggctgtgtt
    20821 tatcggaaat cctatcgttt gataactact tagtttttca ataacgtttg taccttccga
    20881 gtgaacacca aataaattat cgatctgacc taatgcaata atgatagcaa tacctgaagt
    20941 aaaccccgta attacgggtg ctgggataaa agaagtcagt gttcctaatc ggaaaagtcc
    21001 tgctgcaagt aaaaatagac ccgctaaaaa agtagcaagc aatacacctt gcattccctt
    21061 tgttgccgca atagacatca agatagctgc catcgcacct gtcggacctg atatttgata
    21121 aaaaccgcct gataaaccgc caataacgat accggcaatg atagccgtaa tcaaacctgc
    21181 ggccgcatca gctccgcttg ataccccaaa agccaaggct aaaggcaatg ctacagcagc
    21241 tactgtgatc ccagccaaaa gatctttctg aaatcttgaa agatcataac ctgaaaattc
    21301 tttttttagt gaggaaacat aatttttgaa cacctatcta actccttttt catgaaattt
    21361 tc
//
LOCUS       DAZKOW010000048        20716 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000048 DAZKOW010000000
VERSION     DAZKOW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.048"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            352..1041
                     /locus_tag="ACG912_001648"
     CDS             352..1041
                     /locus_tag="ACG912_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0512971.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="ACG912_001649"
     CDS             1028..2236
                     /locus_tag="ACG912_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0512972.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="ACG912_001650"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="ACG912_001650"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFQ0512973.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG912_001651"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="ACG912_001651"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFQ0512974.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="ACG912_001652"
     CDS             complement(6339..7034)
                     /locus_tag="ACG912_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFQ0512975.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG912_001653"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="ACG912_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFQ0512976.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="ACG912_001654"
     CDS             complement(8233..8424)
                     /locus_tag="ACG912_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFQ0512977.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="ACG912_001655"
     CDS             complement(8445..9335)
                     /locus_tag="ACG912_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFQ0512978.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="ACG912_001656"
     CDS             complement(9322..10089)
                     /locus_tag="ACG912_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0512979.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG912_001657"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="ACG912_001657"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFQ0512980.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG912_001658"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="ACG912_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFQ0512981.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG912_001659"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="ACG912_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFQ0512982.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="ACG912_001660"
     CDS             14660..15043
                     /locus_tag="ACG912_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0512983.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG912_001661"
     CDS             complement(15211..15969)
                     /gene="jag"
                     /locus_tag="ACG912_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFQ0512984.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="ACG912_001662"
     CDS             complement(15987..16805)
                     /locus_tag="ACG912_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFQ0512985.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG912_001663"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="ACG912_001663"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFQ0512986.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG912_001664"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="ACG912_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFQ0512987.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG912_001665"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="ACG912_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFQ0512988.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG912_001666"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="ACG912_001666"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFQ0512989.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5931 a         4223 c         3801 g         6761 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtgtattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca cctttt
//
LOCUS       DAZKOW010000049        20661 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000049 DAZKOW010000000
VERSION     DAZKOW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.049"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            39..407
                     /locus_tag="ACG912_001667"
     CDS             39..407
                     /locus_tag="ACG912_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFQ0512990.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            577..1008
                     /gene="mraZ"
                     /locus_tag="ACG912_001668"
     CDS             577..1008
                     /gene="mraZ"
                     /locus_tag="ACG912_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFQ0512991.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1029..1988
                     /gene="rsmH"
                     /locus_tag="ACG912_001669"
     CDS             1029..1988
                     /gene="rsmH"
                     /locus_tag="ACG912_001669"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFQ0512992.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            1993..2394
                     /gene="ftsL"
                     /locus_tag="ACG912_001670"
     CDS             1993..2394
                     /gene="ftsL"
                     /locus_tag="ACG912_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFQ0512993.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2395..4587
                     /locus_tag="ACG912_001671"
     CDS             2395..4587
                     /locus_tag="ACG912_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0512994.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4613..5575
                     /gene="mraY"
                     /locus_tag="ACG912_001672"
     CDS             4613..5575
                     /gene="mraY"
                     /locus_tag="ACG912_001672"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFQ0512995.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5589..6950
                     /gene="murD"
                     /locus_tag="ACG912_001673"
     CDS             5589..6950
                     /gene="murD"
                     /locus_tag="ACG912_001673"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFQ0512996.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            6976..8064
                     /gene="murG"
                     /locus_tag="ACG912_001674"
     CDS             6976..8064
                     /gene="murG"
                     /locus_tag="ACG912_001674"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0512997.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8079..9299
                     /locus_tag="ACG912_001675"
     CDS             8079..9299
                     /locus_tag="ACG912_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFQ0512998.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9455..10780
                     /gene="ftsA"
                     /locus_tag="ACG912_001676"
     CDS             9455..10780
                     /gene="ftsA"
                     /locus_tag="ACG912_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFQ0512999.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10803..12044
                     /gene="ftsZ"
                     /locus_tag="ACG912_001677"
     CDS             10803..12044
                     /gene="ftsZ"
                     /locus_tag="ACG912_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFQ0513000.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12061..12738
                     /locus_tag="ACG912_001678"
     CDS             12061..12738
                     /locus_tag="ACG912_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFQ0513001.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12752..13351
                     /locus_tag="ACG912_001679"
     CDS             12752..13351
                     /locus_tag="ACG912_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFQ0513002.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13432..13653
                     /locus_tag="ACG912_001680"
     CDS             13432..13653
                     /locus_tag="ACG912_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFQ0513003.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13702..14484
                     /locus_tag="ACG912_001681"
     CDS             13702..14484
                     /locus_tag="ACG912_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFQ0513004.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14505..15212
                     /locus_tag="ACG912_001682"
     CDS             14505..15212
                     /locus_tag="ACG912_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFQ0513005.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15474..18272
                     /gene="ileS"
                     /locus_tag="ACG912_001683"
     CDS             15474..18272
                     /gene="ileS"
                     /locus_tag="ACG912_001683"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFQ0513006.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18321..19847)
                     /gene="zwf"
                     /locus_tag="ACG912_001684"
     CDS             complement(18321..19847)
                     /gene="zwf"
                     /locus_tag="ACG912_001684"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFQ0513007.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19862..20509)
                     /locus_tag="ACG912_001685"
     CDS             complement(19862..20509)
                     /locus_tag="ACG912_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFQ0513008.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
BASE COUNT         6983 a         3575 c         4418 g         5685 t
ORIGIN      
        1 gtatacttga agcaacgata aaaaaggagt ttacgttcat gggcacattc acaccactta
       61 tcttgttttg gtatatttac ccggtaatcg ttttgtttgg ttgccaattt cttgtaaaat
      121 tattttcgtt aaatcgtcgt tttaaaataa aagcccctga tttggcgatt ccttttttgt
      181 ggggcggtat gcatgcattg tctagaaata cagggacaat ttctatctta ccgtttttct
      241 ttatttctgt tttgctaatg ggaatttgta ttgctgtttt ccaagcatat tattatgaag
      301 aaattattta tccgcgttat ttcaagatga cttggcgttt ggtttttctg ttgacaatcg
      361 tcttgtacgc tgtcttgatc attgtgaaca tactttctta cctatgaaaa aaatagaaaa
      421 gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt tttaaaaaaa tgtttatgaa
      481 taaaaaaagt tgttcccgga tttcccagaa aaggtggtag aaagtggggg attgtggtag
      541 actaatttta gttagtggag gaatgggggg ctttcaatgt tgatgggtga attccaacat
      601 aatattgatg caaaaggacg tttaatcgtg ccctcaaaac ttcgtgaaga acttggcgaa
      661 aagtttgttc tgacacgcgg attggatggc tgtcttttcg gctatccgat gagcgaatgg
      721 gaaaatctgg aagctaaact gaacgaaatg ccgttggcta aaaaagacgc acgtactttt
      781 gtgcgattct tctattcggc tgccactgaa tgcgaacttg ataaacaagg acgaatcaat
      841 atacccagca cgttaagaaa ttatgcagct ttgacaaaag gttgcgtagt catcggtgta
      901 tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag atttttctgc agcagcagaa
      961 gagaactttg acgaaattgc tgaaagtatg attgatttcg gactataaga ataggaagag
     1021 gctgactaat gactgaaaca tttcaacact atacagtcat gttgaaagaa acggtagatg
     1081 gattgaacat caaaccggat ggcgtttatg tagactgtac actcggcggt gccggacata
     1141 gtgaatattt attaagccaa ctaagttcta aagggcatct gtatgcgttt gaccaagatc
     1201 aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta tgtcgaacaa ggtatggtca
     1261 catttatcaa agcgaacttc agagaattag aagaacgctt gagtgagcat gtagataagg
     1321 tagacggcat tttgtatgat ctaggggttt cctctcctca gctagatgaa gcagaacgtg
     1381 gatttagtta tcatcaagat gcaccgttgg atatgcgcat ggatcagtca gctcctttat
     1441 ccgcttatca cgtaattaat gaatattcct accatgaatt agtaaagatt ttcttccgct
     1501 atggcgagga gaaattttcc aaacaaattg ctcgtgaaat cgaacgagta cgaaaaacaa
     1561 aaccaatcca aacaactggt gagcttgtcg aaatcattaa acaagtcatc ccagctcccg
     1621 caagaagaaa aggcgggcat cctgcaaaaa gaatttttca agctgtgcgg attgcagtaa
     1681 atgacgaact cggtgccgaa gaggcctctt tagaacaggc aatccggtta ttgaaagtca
     1741 atggaagaat cagcgtaatc acattccatt cattggaaga cagaattgtc aaaagtatgt
     1801 ttaaagaata tagtacggta caagatctac caccgggact tcctgtcata cctgacgaat
     1861 tccaaccaga actgaaagtc attactcgta aaccaatcct tccttcagag gaagagctaa
     1921 cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc agaaaaaata agaatgaaag
     1981 aggagtagaa tgatggcaga attaaagaaa atgcaagatt acgaatatga tttacctgaa
     2041 ttggtggatc agccggaaca acaaatgcct gattcttctt cgaatcaaga aatcattctt
     2101 ccacaatcac cggcaaaacg attaaagcag atttctgctt tggaaaaaat tgctgtggca
     2161 agcgtggcga ttgctattgt tgctttatgt gttttaacag ttatgctgcg cacaaatatc
     2221 agcggagctg aaaaagaaat cacgtcaata caaacagaaa tcaatgataa aaagcaagaa
     2281 aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta ctgaacggat taaacaaatc
     2341 gcagaagaaa aaggattgtc tattaatgac gacaatttaa ggaaagtgaa gtaaatgagt
     2401 cttaaaaata aaattcggcg gtttatggaa aagaaaaatc taaatccgat gaacaatcgc
     2461 aaaaaagtag gtatcatttt atttgctacg agtattggat tgttcttttt atttgccgtc
     2521 cgtttctcgt atatcgttat tggaggtcat gttgctggta cctcattagc tgaaaaaaca
     2581 aaacagttgt accaaggaag tgaagtagta aaagcaaaaa gaggaacgat ttatgatcgt
     2641 aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa ttaaagcgat actctcaaaa
     2701 acatatacct ctggggataa aaaactgtat gttgaagaaa agaattttga taaaatagca
     2761 gaaatcctgc ataagaatct ttctatagac aaaaaagatg cactgaatat tttagaggac
     2821 ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct atgggaaaaa tatctcccaa
     2881 gaaaccaagc aaaacatcga agcggacatg aaaaaagaag gtgtcgctgg tctttatttt
     2941 gtcgaccatc aggctcggat gtatccaaat ggcgtctttt cttctcattt tattggatac
     3001 gcagttccag acaaagatga gaatggctta gttggtaaac taggtttgga gtctgcttac
     3061 aatgatatct tgagcggtaa agatggtaag attatttatc aaaaagataa ttttcaaaat
     3121 cctctgccag gtacagtggc agaagaagaa aaagctgtcg acggacaaga tatctacacg
     3181 actttagaca gcagactaca aagttattta gaaacactga tggatcaagt aaatgaagaa
     3241 tatcagccag aagaattgac agcagtcctg atgaaagcaa aaaccggaga aatcttggca
     3301 atgggacaac gacctacttt caatcctgaa acaatggaag gattgactgg aaaagatgct
     3361 atctggcgta atttcttggt acaagatagt tatgaaccag gatcaaccat gaaagttttc
     3421 actactgcag ctgctatcga agaaggagaa ttcaacgaga atgaaacctt ccaatctgga
     3481 aaaatccaag tagaagatgc tacgatcaat gaccatgact ttggtgaaaa aggtgtgctg
     3541 accatgcgtc aagctctttc atggtcaagt aacgtcggga tggtcatact ggaacagcga
     3601 ttaggcggac gatggtataa ctatttacaa aaactgggat ttggacaaag tactcattct
     3661 ggattagatg atgaagtaaa cggtgcttta cctacattaa atattgtcga ccgagcaatg
     3721 agtgcttatg gacaagcggt aggtgtgacg aatttccaaa tgatgaaagc tttcacttct
     3781 attgcgaata acggtacaat gattcaacct cgatatatca gcaaagtggt ggatccccaa
     3841 actggagaag agcgaacaac tcaaacagaa gttctagggc aacctttttc aaaagaaaca
     3901 acagaaaaag tccgtgaata tatgagagat gttgtcgaaa gcgaaaacta cggtagtgct
     3961 tatggtgtat atagtgtacc tggatacaat gtatcggcaa aaaacggaac agcccaaatc
     4021 gcttctgata cagggggata tcaaacagga gatacagcct acctgtattc gattgtggag
     4081 atggttcctt ctgaagatcc agactatgtc ttgtatctaa caatgaaaca ccctaagaca
     4141 tatgatcgaa tggcattggc taaaatcgca aatccattga tgaaacgggc aatggatttt
     4201 aaagaaactg aagaagattc agatacagaa acaaaaacag aaaaagtatc cgtagccgat
     4261 tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc aaaaaagtgg actgcaacct
     4321 gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta ctgccaacgg tgatcagttg
     4381 atctctggag aaaaactgat cctatatact ggtggtgata agctgatgcc tgacgtaaca
     4441 ggatggtcaa aagcagatat catgaagctg gggaaaatcc tcggtattga agttagtttt
     4501 gatggagatg gttattgtgt caaacaagaa cttgctccat atgaaaaaat cactgaagac
     4561 aaacttagtt ttactttaga agaataagaa gagaaatagg agagaaaaga gcatggaatg
     4621 gacacaagca ttgattccca ttgtcagcag ttgtgcaatg acaattgccg caatgccgct
     4681 atttattggt tattttcaaa tgaaaaagca aggtcaggcg attcgtgagg agggaccaaa
     4741 gtggcacaat agcaaagccg gaactcctac aatgggcggc ttagtgtttc tgatcggctc
     4801 tattttgaca ggaatttggg ttggagcttg gcaaaaacag ctaactccta ctcttttcat
     4861 cttgcttttt gtattagcgt tatacggagt aatcggcttt cttgatgatt ttataaaaat
     4921 attcaagaaa agaaacatgg gactgaactc gaagcagaaa ttgattggac agattctagg
     4981 gggaatcatc ttttatcttg tttaccgttc tgaaggttat ccaggtgtat tgaatttctt
     5041 cggactagat ctacctttag gaatcattta tggtattttc gctctcttct ggcttgttgg
     5101 attttcaaat gcagtaaatc tgacagatgg catcgatgga ttagttgctg gcttaggaag
     5161 tatctctttt gcagcttacg gtctaattgc ttggcatcag catcagtatg atgtattaat
     5221 catttgctta agtgttttag gaggtttact gggtttcttc gtctacaaca gaaaacctgc
     5281 caagattttt atgggagatg tcggttcatt agcgttgggc ggacttttag cggcaatttc
     5341 tataatgcta aaccaagaat ggacattgct tttgattggt ttgatctatg ttatggaaac
     5401 agcaagtgtg atgcttcaag ttacttcctt taagcttaca ggaaaacgaa tttttaaaat
     5461 gtctccaatt catcatcact ttgaaatgtg cggatggtcg gagtggaaga ttgatacagt
     5521 tttttggttg atcagcattg tgacttcgtt gatcacatta tggatcgttt ggtaggagat
     5581 gacgggaaat gaaaaaaata tcaacatatg aaaataagaa agtgcttgtc ttaggactcg
     5641 caaaaagcgg agtaagtgcg gccaaattat tacatgaatt gggcgcactt gtcaccgtta
     5701 atgatggaaa accttttgac gaaaatccgg aagcacaaga gctgctttct ttagggataa
     5761 aagtaataac aggtagtcat ccaatcgaat tgttggatga agaattttca ttgatggtca
     5821 aaaatccagg gattccctac agtcatcctt tagtagcaaa agcccaagaa atgggtattc
     5881 cggtaattac ggaagttgag ctagcttacg aagtagcgga atgcccaatt attgggatta
     5941 caggaacgaa cggaaaaaca acgacaacaa cgatgacagg actactactg aatgcaggag
     6001 cagatcaagg aattgcacgt ttagcgggta atattgggta tccagcaagt ggtgtagcgc
     6061 aagaagcaaa atcagaagat aaaattgtga tggaattgtc tagcttccaa ctcatgggaa
     6121 tcacagattt ccgccctcat atagcggtga ttaccaatat ctatgaagca catattgatt
     6181 atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa ccttcaaaaa aacatgacag
     6241 aaaaagatta tctgatcctt aactggaatc agtcagaact acaagaatta gcgcaaagaa
     6301 caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt ggaagacggt gtgtatgctg
     6361 acgattactc aatatactat aaaaaagaaa aaataatgga aatatctgaa cttggcgtac
     6421 caggtaagca taatgtagaa aatgcactag ctgctatttc tgtagcaaaa ttgtatggta
     6481 tatcaaatga agctatacga gaaacgttgc attttttcca tggcgtacca catcgtacgc
     6541 aatatgtagg agaaatccaa ggcagaaaat tttataatga ttcaaaagca accaatattt
     6601 tagcgactaa aatggctcta agtggatttg aaacgtctaa ggtcgtgtta ttggctggcg
     6661 gattagacag aggaaataca tttgatgaat taattccttc attaaaagga atcaaagcaa
     6721 tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc tggaaaaaaa gcaggcatcg
     6781 agacaatagt aacagccgat tctgtagaac aagcagttcc tttagcatta gaaaacagta
     6841 cagatggtga tgtagtttta ttatcaccag cgaatgccag ctgggatcaa tacccaaatt
     6901 ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg attaaaatag aaaaagtaga
     6961 agcgagcgat aaaaaatgaa aattttggtt acaggcggag gaacaggggg acatatttat
     7021 ccagctcttg cctttgtcaa ttatgtaaag actcaagaac cgaatgctga attcatgtat
     7081 gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg atacaggaat gcctttccat
     7141 accttggaga ttcaaggatt taaacgaaaa atttccatgc ataacatcaa gacggtccga
     7201 ttgtttctta agagtatccg tcaagcaaaa aaaatcctca gtgattttca accagatatc
     7261 gttatcggta ctggaggata tgtttccgga gcggtagtgt acgctgcagc caaaatgggt
     7321 attccaacag ttatccatga acaaaacagt gtaccaggtg ttaccaataa atttttaagc
     7381 cgatatgtgg atcggatcgc tctttcattt aaagatgcgg cttccttttt tcctgaaaac
     7441 aaagcagttc ttgtaggtaa tcctcgagca caagaagtag cagatactaa aaaatcagat
     7501 attttgaaaa catttgattt ggatccagaa aagaaaacag tcttgatttt tggcggtagt
     7561 caaggagcac ttaaaatcaa tcaagcagta tcagtttttt taagtgaata tattccttct
     7621 tcttaccaag ttttgtatgc ttcaggagat agatattatg aagagatcct aaataaaata
     7681 aatcctgaaa tacaaaatat tagtgtccag ccttatatca aaaatatggc agaggtcatg
     7741 gcttccagtg atctgctggt ggggcgtgct ggtgcaactt cgatcgcaga attcacagct
     7801 ctggggttac cggcagtgtt ggttcccagc ccatatgtta cgaatgatca tcagactaaa
     7861 aatgcaatga gtcttgttca cgcaggagca gcaaaaatga ttgcagataa tgaactaaca
     7921 ggtgaatcac taagtcagac agtcaacgaa atcatgggtg atgaagaatt gcaaaagcaa
     7981 atgtgccgag cttctaaaga acaaggaatc cctgatgctt caaaacgtct ttatgactta
     8041 gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt gagtaagaaa aaagaaccaa
     8101 aaaaattgtc tcatccgtct tctgatgaaa acaatttgac tccttggcaa aaggctaacc
     8161 gaaaatatct ggcagaacaa gagatgaaaa accaagagcc aaaaaaagaa gacataacat
     8221 tagatacacc cctttcagat tctgaagaag aaaaaacggt tcaagaggaa cagcaagaag
     8281 aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat caagcaaaag aaaaacggtg
     8341 gaccatataa cggttctttt ttgaaccggc tgcccaattt aaaaagtcaa cggcacaaag
     8401 tactatttcg tcggttagct ttgatcatca ctgtattggg tataccgctg atttttttga
     8461 tctattatgt ttctccttat agcaaattac aagcagttgc agtatcaggc aataagacag
     8521 tcaacagcca agaaatcatc tctgatacaa aattgtcatt aggagaaaat gtttggggac
     8581 agtattttca tcgatctact tatatcgatc gccttaaaaa agcacagcca agaatcgaaa
     8641 cagcaaatat acatttcaaa gggatgaatg aattcgattt ggatgtaaca gaatataaag
     8701 agattgcttt gatcgcaaaa aacaatcaat actacccagt gattgaaaat ggaacagtat
     8761 tagatgaaaa agtagcaaat cctacaaaaa atctccctat cttggaagag ttcaaagact
     8821 ctgcaaaaat caaagaatta acaaagcaat acaatcagtt atcatcagaa ttacaaaaag
     8881 caatttctga aatcaagtac acgccaagag caagcaataa aaatctgatc caattgaata
     8941 tgaatgacgg caatcaagtt attgtaaata tcaataatct tgcgaaccaa atgaaatatt
     9001 attcgcaagt agccaaagat atggatgaaa aaggtgtgat cgacatggaa gttggtatct
     9061 tctcgcatcc ttacggtagc actcaagaaa cggaagaatc gggccaatcg gaagaaacaa
     9121 ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag tgaagaggca gaaacgcaaa
     9181 aagcacaaac acctggatcg tctagcacaa acggatcatc tgattcaaat gattcaagtg
     9241 aaacaagtca aatagaagat aatccaagta caactccgtc aagtttaaat gaaaattgat
     9301 aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc agtaatttat ggtagaattg
     9361 gaagcagtga atatatctat taataagtat attttaaaac ggacgctagt tgttcgcttg
     9421 tatatggaat agtaagtagg agggaatccc attcatggca aaaacaggaa tgtatgtagg
     9481 ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct gaatatatcg acagccaaat
     9541 gaatattatt ggagtaggaa acgcaaaatc agaagggatt aaccgaggca ttatcgttga
     9601 tatcgataaa acggtccaag cgatacaacg agcagtacga caagcagaag aaaaagcagg
     9661 tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac atgcttgaag tagaaaattg
     9721 ccagggaatg attgcagtta acggagattc aaaggaaatc accgatgaag atgtcagaaa
     9781 tgtggcttca gctgcccttg ttcgttcaat acctcctgaa cgtcaaatcg tgtcaatatt
     9841 gccacaagat tttactgtcg atgggttcga gggaatcaaa gatcctcgtg gaatgatcgg
     9901 cgtaagatta gaaatgtacg gtctgttgtt tacaggacct aaaacgatca tccataacat
     9961 acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa atggtgatta ctcctttagc
    10021 gctgactgaa tcaattcttt ctgacggaga aaaagatttt ggtacgatcg tcattgatat
    10081 gggtggcggc caaacaacaa cggctgttat gcatgataag caattgaagt ttaccaatct
    10141 tgaccaagaa ggtggcgaat ttgtcactaa agatatctct attgtattaa atacgtcatt
    10201 taacaatgca gaagcgttga aaataaacta cggggatgca tacccagaac ggacatcacc
    10261 agatgaagaa tttccagtag atgtgattgg tcagtcagaa ccagtgaaag tcgatgaacg
    10321 ctacttgtca gaaattattt cagctcgaat ggaacagatt ttcaacaaag caaaagatgc
    10381 attggaccaa atcgaagcat tagagttgcc aggtggcgtc gtattgacag gcggagctgc
    10441 tagtcttcct ggtgttgtgg acttggcaca agaaatcttc ggcgtaaatg taaaattgta
    10501 tgttccaaat cacatggggc tgagaaaccc ggtatttaca aatgtaataa gtatcgtgga
    10561 ttattcagca aacttgagtg aagtgtatca attggcaaaa attgctgtta cgggtgaaac
    10621 ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca aacacatatg agagctatga
    10681 agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa gagtccggag aaaatgtaaa
    10741 aaataaaatc aagggtttct ttacaaatat ttttgattaa ttcatcctgg gaggaataag
    10801 atatggaatt ctcaattgat aataatatca atgacggcgc agtaatcaaa gtcatcggag
    10861 tcggtggtgg cggtggaaat gctgtaaacc gaatgattga agaaaacgtt aaaggtgttg
    10921 aattcattac agccaacaca gacgtgcaag cattaaaaaa ttcaaaagct gaaacagtta
    10981 tccaattagg ccccaaatat actcgtggtt taggtgctgg ttctcagcca gaagttggac
    11041 aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc tttagacggt gcagatatga
    11101 ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg tgctgcacca atcgttgcag
    11161 gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt gacacgtcca ttcacttttg
    11221 aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc tcgtttgaaa gaaaatgtgg
    11281 atacattatt aattatctca aataaccgtt tattagaagt tgtagataaa aagacaccga
    11341 tgcttgaagc tttccgtgaa gcagataatg ttttacgaca aggtgtacaa ggtatctctg
    11401 atttgatcac agcgccaggt tacgttaacc ttgactttgc tgacgtgaaa acagtgatgg
    11461 aaaaccaagg tacagcattg atggggatcg gtgtggctag tggagaagat cgcgtagtag
    11521 aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac atctattgat ggagctgaac
    11581 aagtacttct taacatcaca ggcggtttgg atatgacatt gttcgaagca caagatgctt
    11641 cagatatcgt agcaaatgca gcaactggcg atgtaaatat cattctaggt acatctatca
    11701 atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc aacaggtatc gatgaatcaa
    11761 aaaaggaacg caagtctagc cgaccagcta gacaagcaca aatgcaatca cctgctcaaa
    11821 agactgtatt agatatggat caagctaaac caacttcttc agaagaagaa aacagttttg
    11881 gcgattggga tatcagacgt gaacaaaatg ttcgtcctag agttgatgat tccaattttg
    11941 acaatatcga aaaaaaagaa tttgatacat tcaaccgcga agaaacaaaa tctcatggcg
    12001 atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa ataaaggaga ggaagagcac
    12061 atgattgctg acaacttgca gaaaatcaag caagaaattc aggattcgtg tgctcttgtt
    12121 catcgtgatc cgaaagaagt tacattagta gctgtaacaa aatctgttga atccgctcaa
    12181 gctgaagaat tggttagagc tggcgttatg aattgtgcag aaaaccgagt agataaatta
    12241 ttagaaaaga aacaagattt atttgatttt cctgaaataa agtggcattt aattggtaat
    12301 ttacagcgca gaaaggtaaa attgattgta aatgaaattg attactttca tgcgttggac
    12361 agtttaagat tagcagaaga aatccagaag cggttagaga aagaattatc ctgttttatt
    12421 gaagtaaatg tttctggtga ggaatcaaaa catggaattt caccagatga gcttttaccg
    12481 tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg ttgggttgat gactatggcg
    12541 cctaaagaag cttctgatga gcaaatccat aaagtgtttg gtacgctgaa agaattgcgt
    12601 gatcttgttc aagcaaaagg ttatgcccat gcaccatgta gtgaattaag tatggggatg
    12661 agtcaagact ttcaaattgc gattgaagaa ggtgcaacat ttatccgtgt aggaacagcg
    12721 ctattcaaag acgaataaag gaggaacaaa aatgccgctt ttaaacagag gagcaattgc
    12781 taattttttc ggattagctg atgaggaaga gtacgaatac caagattatc ctgaaacaac
    12841 aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag aatcaatcaa ctcattctgt
    12901 atctgctgcg aatactcatg aaaaacaaaa aacggcgact tcacgtcctg taacgcatga
    12961 aagtattcag ccggctgaaa agaaaaaagt aattgaacta catcaatcac ctacaaaaac
    13021 tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa cctaaagcaa atcttgctgg
    13081 aaaaatcact gtaatggaac caagagctta ttctgaagct atggcgattg ctaaacgaat
    13141 cattgctgga gaagcaacct tagtgaattt tcatttagta gaagaaaaac aagcgagaag
    13201 aatcgttgat tttctaacag gaacagttta tgcactagat ggagatatca aacgtgttgg
    13261 agatgagatt tttctttgta caccgactgg gatggagatt gacagttcaa ccgctcaatc
    13321 ttttgcagat gcgaatttgt ttgatctgta agaggaggaa ctacgattta tactattgca
    13381 ctattacttc atttacttca acgggcagcc tatttctata gcattatctt gatggtatat
    13441 gcattgctct cttggtttcc aggcgggtat caatcagggc taggacgatt attagctaag
    13501 atctgcgaac cgtatatcag tttgttcgat cgtctgcctt tgaggattgg acccttggat
    13561 ttcacaattt taggggcaat cctgatcttg aacttagccg tacaaggttt agcacagatc
    13621 gttatatgga taactgtcca tttagtttat tgattgaagc aacaaaatct ttattctcag
    13681 aatagatagg aggggaaaat tttggatgca aatgtatacc agcactttcg ggcagatgaa
    13741 cgtacattta tcgattcggt aggcgattgg attgaacagg ttcaaggaca atatgcaccc
    13801 tatctgaccg aatttcttga cccaagacaa tcctatattt tagaaacact gattcgacaa
    13861 agcagtgatt tgcgtttcat gttttatggc ggatatgaac aggcagaaag aaaaagatgt
    13921 ttgattttcc ccgattatta cgaacctgta gaggaagatt ttgaaacagc attgtatgaa
    13981 atccattatc ctagcaaatt tgccagccta agtcatggga aaatccttgg aactttagtg
    14041 ggaacgggga tcaaacgctc ttactttggc gatatcatct cggacggtga tcgctggcaa
    14101 gtatttattg caaatgaggt ggatcatttt gttgctgcac aagtaacaaa gattggaaag
    14161 gttgctgtac gcttggaacc aatcaaatac acagaaatga ttatgccgaa agatagctgg
    14221 acacaagaaa gaggaacagt tagttccatg cgactagaca gtgtgatctc tgagatttat
    14281 aatatttcca gacagcggtc caagcagctg atcgaagctg gtaaagtaaa agtgaactgg
    14341 acagaaaata caagacctga ttttgtactt gaactattag atattatttc agtcagaggt
    14401 tatggcagag tccagatcca agaacttgag gggaagacga aaaaagaaaa gtacagggta
    14461 ctgttcggtg tattacgtaa ataaaataaa aaggtggttt actcatggct ttaactccat
    14521 tagatatcca aaataagaca ttcccgacaa aaatgcgggg atataatcaa gacgaagtag
    14581 atgatttctt agatttagtt gttcgtgact atgaggaatt gactcaaaga aatcgagaat
    14641 tagaaaaagc agtcaaacat tctgaagaaa aacttgagta cttcaatgaa ttaaaagatg
    14701 ctttgaacca atcaatcatc gtagctcaag atacggctga taaagtaaaa acaagtgcaa
    14761 gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa agcagatgag ttagttgcga
    14821 acgctgaaaa acgtgcacat caattaacaa ctgatgcaga agaaaaagca agaaaaatct
    14881 tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga aactgaagat cttaagaaaa
    14941 aaacacgagt attccatcaa cgtatcagct tgatgcttga gtctcaatta gaacaagtga
    15001 aaagtccgga atgggatgaa attttacaac cattctccag ctacgtgact gacagccatg
    15061 aagtgatcaa agaagtttta gcaaaacagc ttgacaatga aaatgatact gatgtaaact
    15121 cagacacagg agtcatagaa gcacctatga cggcatttga tacacaggct attgatatct
    15181 cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa agcactttgt tattcttaaa
    15241 gcgagccgat gagagtggaa gttcggtaag acactgcatg gtgaaagatc ctctacttga
    15301 gacctgcttt tccgaaatta gagtaagaag cagcggctga cctcgttatt ggtcacagag
    15361 agtttgcact agatagatgc aactaaattt gggtggtacc acggcatgtt cgtcccttgc
    15421 ggctgaacat gcttttttgt gtccggaaaa caaataaagg agtgtatggt tgaatgaaaa
    15481 tgaaagaaac cttgcaatta ggaaaaacaa aattccctat gcgtggtaat ttgccaaacc
    15541 gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat ctaccaaaaa agacaagagt
    15601 tgaacgaagg aaaacctagc tttgttcttc atgatggacc tccatatgcc aatggtaata
    15661 tccatttagg acattcattg aataaaatca gtaaagacat tatcattcgt tcaaaatcaa
    15721 tgtctggcta tcgtgcacca tatgttccag gatgggatac acatggattg ccgattgaac
    15781 aagtattggc taataaaggc gtgaaacgta aagaaatgtc catggcagaa tacttggaaa
    15841 aatgtcgtga atatgcactt tcccaagtag ataagcaacg agcagatttc aaacgcctag
    15901 gagttgcagg agattgggaa catccttatg ttacattaga tccttcttat gaagctgcac
    15961 aagtacgcgt atttggtaaa atggctgaaa aaggctatat ttacaaagga ttgaaaccaa
    16021 tttactggtc tccgtcaagt gagtcttctt tagcggaagc tgaaattgaa tataaagatg
    16081 taaaatctcc atctattttt gtagctttca atgtcaaaga cggcaaagat attcttgatg
    16141 aagatacagc ttttgtcatt tggacgacaa caccatggac attgccagca aaccaaggta
    16201 ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc cgatggccgt aagtttgtcg
    16261 tagcgaaaga tttgctagaa acagtccaat cagaaatcgg ttgggaaaat gtaacagttc
    16321 taaaagaatt tgctggacaa gaaatggaat atatgactgc aagtcatcca ttctatgaac
    16381 gtgaatcact tgtgatttta ggcgatcatg ttactttaga cgctggtact ggattagtcc
    16441 atactgctcc aggacatggg gaagatgact atatcgctgg tagcaaatat aaactatctg
    16501 tagtgtcacc tgtagactca aaaggattgt ttacagaaga agcacctgga tttgaaggta
    16561 tcttttatga caaagcaaat cctatgatca ctgatctatt aaaagaaaaa ggagctttgc
    16621 tgaagctaga tttctttact catagttatc ctcacgattg gcggacgaaa aagcctgtga
    16681 tctatcgggc aactccacaa tggtttgcat ctatcgataa attccgtcag aatattttgg
    16741 atgaagtaga aaaagtagag tgggttatcc catggggtaa aacacgtttg tataatatga
    16801 tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc atggggagta ccacttccga
    16861 ttttctatgc tgaaaatgga gaagcaatca tcacaccaga aacaatcgaa catgtggcaa
    16921 aattatttgc tgaacatgga tcaattatct ggtttgaaaa agaagcgaaa gaacttcttc
    16981 ctgaaggatt tacccaccca ggttctccta atggcgaatt tacaaaagaa aaagatatca
    17041 tggatgtctg gtttgattct ggttcttctc atgaagctgt tttacgtcag cgaccagaat
    17101 tatcattccc agcagatatg tatttagaag gatcagacca atatcgtggc tggttcaact
    17161 caagtatcac gactagtgtg gctatcaatg gtgtagcacc atacaaatca gttatttctc
    17221 aaggatttgc tttagatggt gaaggccgta agatgagtaa atctttagga aacatcattg
    17281 caccagaaaa agtgatcaaa caatttggtg cagatatcct tcgcttatgg gtatctagtg
    17341 tagattatga agcagatgtt cgtgtttcta tggatatctt aagtcaagtg gcagaagtat
    17401 atcgtaaaat cagaaatacg atgcgtttct tattagcgaa tacagaagat ttcgatccta
    17461 aaaaagatac tgtttcgtac aatgatttac gttctgtaga caaatatatg accgttcgat
    17521 tgaaccaagt aatcaaagaa atccgagaag aagggtacga caaatataac ttcatgcata
    17581 tttaccgtac agttatgaac ttcttgacgg ttgatctatc gtcattctac ctggattttg
    17641 ctaaagatgt agtttatatt gaagcagaag atgattatca acgtcgttgc atgcaaactg
    17701 ttttctatca aacagctgta gctttgacaa aattattgac tcctatcatt ccgcatacag
    17761 cagaagaaat ctggtcattc ctgaaagaag aagaggagta cgttcagtta agcgagtttc
    17821 ctggttatga agagtttgca aaccaagaag aattgatgga tacctggact gctttcatgg
    17881 acttccgtga taatgtctta aaagcattgg aagaagcaag aaacagcaaa ttgattggta
    17941 aatctttgga agcaaaacta actgtttatc caaaacaaca agttcgtgaa atgttaaaag
    18001 ctttggatgc agatattgca caattattga tcgtttcacc agactacttt gaagttatgg
    18061 cagcagatga acaagtgcca gataacgcta tggtatttga tgatgttgct attacagtgg
    18121 aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt tcgtaaagat gtaggagtag
    18181 atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat cgttgaagac ttctatcctg
    18241 aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa agggggtgtg acatgttctt
    18301 tgtcacaccc ccttttgtta tcaatcgttt aattttccac gttcacgata ccagttatct
    18361 ggttgccaga tccattcgaa tccgtctttt tcaagtagtt cgaaagctgc tttaggtccc
    18421 attgatccag ctttgtagtt gggaaaatcc ggttgtgttt ggtcccaagc ttttcgaatg
    18481 acatccacga tatgccaaga ttgagcgact tcttcccaat gagagaagtt cgttccatca
    18541 ccattcaacg catctaatag taatttttcg taagcttctg ggctattttc aaccatttct
    18601 gcactatttc taaattccaa tttgattggt tccgtttcaa atccttgccc tacttctttc
    18661 ccattcaagg ataaagaaaa tccttcagtt ggctgaatat agatcgttag aacattaggt
    18721 ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt tgaatacatt aacaggaact
    18781 tgtttgaata cgatgttgat ccgagtccct ttttcagtca aacgtttgcc tgtacgtaca
    18841 tagaaaggaa cacctgacca acggaagtta tcgatagtga atttgcctgc gacgaatgtt
    18901 tcagtctctg attgagcatc cactttgtct tcttcgcgat agcccttgaa ttgtacacca
    18961 tccaattcac cttgagcata ttgcccgcga acgaagtttt ctaacacttc ttcttcgtta
    19021 taaatgcgga tagctttcaa tgctttgatc ttttctgtac ggatttcttt ttcggaaaat
    19081 gctactggcg gttccattgc aagtaatgct acaacctgta aaatatgatt ttgcaccatg
    19141 tctttaagtg cgccactatg atcgtagtag cccccgcgat cttctacacc gaggttttcg
    19201 ccaaaagtaa tttggatatt gtcgatatag cgattgttcc attgagcctc aaaaatatta
    19261 ttggcaaaac ggatagctga gatgttttgg atcatttctt ttcctaaata atgatcaatc
    19321 ctgaaaatat cttgttctgg gaatacttct ctgatttgct ggttcaattc aaaagcagac
    19381 tggtaatcag aaccgaacgg tttttctatg atcaaacgat tgaagccatt ttttgtcata
    19441 atattttgtg atttcaaatg agaaacgatc gttccaaaaa attgaggtgc catagccaaa
    19501 taatagacac ggttaccgtt taattgatag tgtccatcta atcggtcaga caattctttt
    19561 aatgtctgat aatgcgttga atcatttaca tcatgggatt gatagtaaaa atggcttgca
    19621 aaggtagttg cttcttcatc tgaaggattc aatgcacgga ttgtttcttt gacaacttca
    19681 cggtagtgct catcgctcca gggacggcga gcggttccga ttacagcaaa atgctcgcct
    19741 aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca atttacgctg ggctaggtca
    19801 cctgtaccgc cgaatatcgt aaacaataca tttttttcat tagacatgtg gatacactcc
    19861 tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa gctgacgggg attttctttc
    19921 tatcattttg gatagtgatc ggtccttcat aagctgcgat gtcaacgatc tcatattctt
    19981 cgttgatatt caggtcaata gtgactaagt aatccaacaa ttctttttca tcaagcactc
    20041 gtgcgatacg cacttttgta ccgacaggat aggactccaa tgtttgtcgg cgttcttcat
    20101 ggacaacttc ctctttttta ggaatcgatc ctccatgagg acaatgttca ggatgattca
    20161 aatagttttc taaatgatct gcaagatgtt ccgaagtcac atgttccaat acttcagcat
    20221 catcatggac ttcattccag ctatagtcta aatgttcaac taaaaaaact tcccataagc
    20281 gatgtttgcg aatcagactg ctagcttttt gcagtccgct tgcagtcaat tgtacacctt
    20341 gataagggga atgttctaca agctcttctt tcacaagttt agaaatcatt tcactgacag
    20401 atgcagctga gacatctaga ctagatacga tttgtttatt gttgactttc gttgtatctc
    20461 cacctagttc taaaatgatt ttcaaataat cttctcgatt tggtgtcata ttcgatcatc
    20521 ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa aaaacgttaa ttgactattt
    20581 ttctaaataa aaaacaaagg atttttagac gtttttacaa caaaaaacga aagatgatag
    20641 agaagactca ttctttcgtt t
//
LOCUS       DAZKOW010000050        20407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000050 DAZKOW010000000
VERSION     DAZKOW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.050"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            380..934
                     /locus_tag="ACG912_001686"
     CDS             380..934
                     /locus_tag="ACG912_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0513009.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1107..1544)
                     /locus_tag="ACG912_001687"
     CDS             complement(1107..1544)
                     /locus_tag="ACG912_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0513010.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1592..2539
                     /locus_tag="ACG912_001688"
                     /pseudo
     CDS             1592..2539
                     /locus_tag="ACG912_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2810..3245
                     /locus_tag="ACG912_001689"
                     /pseudo
     CDS             2810..3245
                     /locus_tag="ACG912_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3261..4094
                     /locus_tag="ACG912_001690"
     CDS             3261..4094
                     /locus_tag="ACG912_001690"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0513011.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4339..5364
                     /locus_tag="ACG912_001691"
     CDS             4339..5364
                     /locus_tag="ACG912_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0513012.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5502..5627
                     /locus_tag="ACG912_001692"
     CDS             5502..5627
                     /locus_tag="ACG912_001692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513013.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5652..6767
                     /locus_tag="ACG912_001693"
                     /pseudo
     CDS             5652..6767
                     /locus_tag="ACG912_001693"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6850..7746
                     /locus_tag="ACG912_001694"
                     /pseudo
     CDS             6850..7746
                     /locus_tag="ACG912_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7762..8586
                     /locus_tag="ACG912_001695"
     CDS             7762..8586
                     /locus_tag="ACG912_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0513014.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8618..9928
                     /locus_tag="ACG912_001696"
     CDS             8618..9928
                     /locus_tag="ACG912_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFQ0513015.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10076..11335
                     /locus_tag="ACG912_001697"
     CDS             10076..11335
                     /locus_tag="ACG912_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0513016.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11345..12217
                     /locus_tag="ACG912_001698"
     CDS             11345..12217
                     /locus_tag="ACG912_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0513017.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12229..13059
                     /locus_tag="ACG912_001699"
     CDS             12229..13059
                     /locus_tag="ACG912_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0513018.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13099..15282
                     /locus_tag="ACG912_001700"
     CDS             13099..15282
                     /locus_tag="ACG912_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0513019.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15343..16563
                     /locus_tag="ACG912_001701"
     CDS             15343..16563
                     /locus_tag="ACG912_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFQ0513020.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16560..18020
                     /gene="gtfA"
                     /locus_tag="ACG912_001702"
     CDS             16560..18020
                     /gene="gtfA"
                     /locus_tag="ACG912_001702"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFQ0513021.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18097..19203
                     /locus_tag="ACG912_001703"
                     /pseudo
     CDS             18097..19203
                     /locus_tag="ACG912_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19525..19734
                     /locus_tag="ACG912_001704"
     CDS             19525..19734
                     /locus_tag="ACG912_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0513022.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19727..19876
                     /locus_tag="ACG912_001705"
     CDS             19727..19876
                     /locus_tag="ACG912_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFQ0513023.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            complement(19952..>20407)
                     /locus_tag="ACG912_001706"
     CDS             complement(19952..>20407)
                     /locus_tag="ACG912_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0513024.1"
                     /translation="TIVGIHHKSAVITLVERLSKAIIALKPEGRKAVDIENSINEWLQ
                     SVPKNLFKSITFDCGKEFSNWKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRKDGL
                     PKEMDFNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT         6942 a         3070 c         3970 g         6425 t
ORIGIN      
        1 aaatatttta ttaatgaaaa gcttggtagc acggttattc atatgctaat ttatttcact
       61 ttatagtaag gggagttctt agtatattgt tcaaacttaa atttcatata atttaattat
      121 gtaaattagc tattctaaga acttctacgg ataactgccc gtctaaaatt taaataagag
      181 cttagaatct tcatataagt aaggttaatt tttttgtaca aaaaagtcgg aatattctga
      241 gttactgaaa tgatataaaa tcataaacta aatcccttga ttttattgac tttcaaaagt
      301 ttatatatat aagttaatga ttatggtaaa atatatacat aatcattaac tcagaatatt
      361 tttaagaagg gaaacaatag tgagctataa tgtacaaccg ttaaggacaa atgaagagat
      421 acaagatttt cagttttggc taagaagaac aagtaatccg gaaagagata gttttttatt
      481 tttgtttgga atcaataatg ggttacgtat gtcagatatt ataggattaa aagttggaga
      541 tatacgaggc aaaagtaagc ctattattgt tgaacataaa acaggtaagc gaaaaccgat
      601 ttttatagat aatttgagag aagaaatttt attatatact gaaggtaaag aagaaaatga
      661 ttggttattt ccgagtcggc aacaaggtcg acatataaca agggatcgtg tctatcagat
      721 atatgcagat atagcagaaa aattaggacg agatgatata ggaacacata cattaagaaa
      781 aacttttggg tatcactact acaagaaaac tagagacatt gctaccttaa tgtttatttt
      841 taatcatagc tcgcaagcaa ttacaaaaag gtacattggt ataactgaag atgaaatagg
      901 agcatcactt cgtggattta aactaggtgt ataatttaaa aatttttata aacagtaaag
      961 acaaagatta aaagctattt ttatctagaa cgattttatt tcataaagtt cgcggaataa
     1021 aagctggggt tactggtact tggtataggt tcgtgttata taaactaagt aattaaataa
     1081 catttttagg ttggaaaagg atgaaattaa aaaaagagaa tcaatacggt ctcaaaaacg
     1141 caaacgctgc ggaataagaa aaatactgat aactcaacgt ttagcagact atctaatagg
     1201 gatagtctgc ttttttcgtt tttgaccata cttttgacct tctgcaaact tccttgcttt
     1261 tcctctgtag aactcatgaa atctgcaaaa cgaattgccg tttcctctgt ctgctttttc
     1321 gcgatatggg tgtatatatc atccatagtg gttttaatat ctgtatgtcc aagtcgaaca
     1381 tacacttctt tgacagacgc accagattca aagagcagac tacaatgggt atgacgaaaa
     1441 ccatgaagtg tgatatgttt taagttattc tctttaatga tgattttcaa attgtgattt
     1501 acatagtcta aataaagggg cttattttct tctgatgaaa acaaaagttg ttcaccatct
     1561 tttataggtt tgattctcat ttttagatat tatgcccgca cgtattcatc acatttctat
     1621 tgtgaatcgc tttattcgac cgacatttga cttttatcat aatatactcg gtttaaaatt
     1681 actaatgaaa acaattaatc aagacgatca taccatgtat cacctgtttt tttcagataa
     1741 tcaccaccgt gtgggaactg aactgacttt ttttgaagta caagaaggga acaaccagtt
     1801 ttttggaacc aatacgattg aacgaacgat actaaaagtt ccttccgaag catcattgca
     1861 tttttgggaa atatattttg aaacacaagg cgtttgccat tatggaatcg aatcattttc
     1921 tggacgacct atcttacgtt ttgaagggcc agatgcaaca cgattagctt tagttccctt
     1981 acgcgaattt gaagatattg atgattattt cccgtatgtc acagaccaga tccctacaga
     2041 gcatgccatt ttaggaattg atgccattca actacgcgta caatattccg atgcgactga
     2101 acgagagtta ttgtcggtag gctggtagca caaagaatac gtcagtttct tttcgggtca
     2161 tcaacgagtg acaatcttag aaaatcaaga cactcagttc tatcaagaag ttcagattat
     2221 tcaagatcgt gagaatccag ttgccattga agggattggt agcgtccatc atgttgcgtt
     2281 tggcgtagaa aataaatctg atttacaacg catcgacaaa caattacaag agcgcaattt
     2341 tataaattca ggaataaaag atcgtgaatt ttttgtatca ctttattatc gtgacccaaa
     2401 tcaattattg attgaaattg ctacagggga aggaaaccta gatgcaaaag cgtatgaaaa
     2461 tcaaagtcca cgctttgaag aaattccttt atttttaccg cagtacttaa atttcattcg
     2521 tgaacaagtg gagcaataat tagctaatca aatccatgac gaagagtaag aggttgactc
     2581 ataagtcaaa aaataaagaa aaacgcatca aatccattct aaagaatgtt gatttgatgc
     2641 ggttcctatt tttataaact atcattttcg actaataaaa ctacttatga gtcagcctct
     2701 ctttttttct tgatttttat acctctttct aggtaaacta aacatctgcc aaaaataccg
     2761 tgtctcattg aaacattctc aactatttca aaataaaggg gagtaccaaa tgaacgagta
     2821 tattgaaatt atccacgcaa gagaaacaac ttaaaaatgt cagtctcaaa attcccaaaa
     2881 ataaaattag tatttttacc agcgtatctg gttcaggaaa atcatcgatt gtttttgata
     2941 ctattaccca agaagctggt cgtcaactga atgacaccta tgatagtttt acccgtttat
     3001 ttttacctaa atacagtcgt ccagaggttg atgagattga taatctttct acggcgattg
     3061 tggtcaatca aaagaagatt ggtggcagtg cgcgctcaac gttagggacg attactaaca
     3121 tcaatgcgtt atttcgtgtg ttgttttcat gttttgcaac acctagttta ggatatgcaa
     3181 atgcttattc atttaatgat cctagtggaa tgtgttctgt ctgtcaggga atcggtaaga
     3241 cgattgcatt tttaatagga ttgctttatt tttcggctat tgataatgct gattttgtat
     3301 tttctgatcc atttaccgca gaagttttgt ctggaattga ggaacaggca aagaatcatg
     3361 gttattttgt attggtgcat aatgttaatt cagcagaaga tgttaaaact attcagagaa
     3421 attggcgttt tgaggggttc attgttgttg gtgtacgtgt aaaagaattt ttggaattaa
     3481 ataacgtttt tgatggtcct gttgtttata ttgatacgca tctttcaaaa gagacattaa
     3541 gagaacttga acaacaaatg aatcgtttat ttgtaaatac aaatgattac caagctagta
     3601 cattggcagt ggaacatttg attcattatg gtcatgagaa aatagcgttt ctttcttttg
     3661 actacattgt aggtgaacca agtgttattc aagaacgaca tcaagcttat atcgatacat
     3721 taaagaaata tcatttaacc tttaaatcgc attggatata taacaactct gaatttgagc
     3781 gaatttatca agaattagct gaatttactg caattgttgt tactggagat tatttagcgg
     3841 ctaaattagt aaaattttta aaaaataaga ataaacgagt gatggaacaa ttatcgatta
     3901 ttggatttga tgatattact tttgcagaat taatggatcc tgcgttgaca acaatacgac
     3961 taaatccagg taacaagggg gcgattgctt ttcaccaact gattggactg gccaaacaag
     4021 aagaaataaa aaatcaagta ctactattag agggagaatt gatttctcga gactctgttc
     4081 acaaaatcaa ataaaaaagc tctgtgaaat aagtatagtt aacaataaaa tatccgaacg
     4141 atagagagaa tgaactgtga tctagcagtt cctcgtcgca aattcactag aattagcatt
     4201 atttactcta aattgttcgg atattttttc ttgtttttaa ttatgattac agtgcctatt
     4261 tttaacgaaa tttgcttatt ttcaaaatgt atgattatat gttagtaaac actttcaaat
     4321 atgaaaagag gagctattat ggtaggaatt agggatgttg caaaacgggc gggtgtgtcc
     4381 attgcaacag tatcgcgcgt gctaaatgag gatccgacac tttcagtaac cgatgaaacc
     4441 aaacgaaaga ttgcagaatc ggttaatttt tatcattatg tcaaaaaaag tcccgttgca
     4501 aaaaatttaa caaatattgc attaataacg acagtttctg aagtagatga gttggaagat
     4561 ccctattttc gaacgattcg cagagggatt caagtcgaag cagaacaaag caaagtcaat
     4621 ttgaagaaaa ttattcgttt gtcagaaaca ccgttggagc aagacgattt gctaaaatgt
     4681 caaggtgtgt tgattattgg gcaggtgcta tcttctgtga ttgaagaagt atcagcaatt
     4741 aattcgaatg tcgtagtgat tgatgaccct agtattgaag ctgaagtaga tgcagtttat
     4801 actgatttaa caaaagcaac gaatcagcat ttagatcgat tgtataaaaa aggacaccga
     4861 aacattgcgt ttatcggagg aggacgtgtt cgtttagata gccaaggaaa taaacacgtg
     4921 tcagatgatg attatcgaaa aatagcctat gagacgtgga tgaaacaaaa aggattagaa
     4981 aaatatagtc aaatttattt agaaggctgg accacacttg acggaatgca ggcagcagat
     5041 ttacttttga aaaaacatgc taaagataag ttgccgactg ctatcatggt aggaaatgat
     5101 cctattgctg tgggggttta tcgttcactt caaaaaaatg gtctacatat tccagaagat
     5161 atttcgattg tgagttttga taatattgaa gttgctgaat ttttaacacc tgcattaaat
     5221 acggtgaatg tgaatacaga agaaattgga aaactagcag ttcgcatggt taatgaacga
     5281 ataaaagaag ttagaaatgt ttcgatcaaa gtgattgttt ctaataatat tattgttaga
     5341 gaaagtgaaa aaaatatcga ttaaaaaagt aaacatccaa aaaagggtgt ttactttttt
     5401 gtttactaat atttttagta aaaaatctcg acaaaacatt aagtaaacag ttatggtgtt
     5461 gttaacaaaa agaaaacgca ttcaaaacag gaggaattaa gatgaaacat ttcaaaaaaa
     5521 tagctttaac agggatagct gttttaactg ggacattact cgcagcatgt ggtgggggag
     5581 atagtaattc taaagacggg caagtaactt taagctatgc gagttgagat actgaccaag
     5641 ctccaggact aagaaaaatg ttagatgaat ttgaaaaaca aaatcctgat atcaaagtag
     5701 aaatggagac aacgccttgg gatcaatatt gggttaaatt agaagctgca acaacgggag
     5761 gtaatatgcc agatgtagta accatgcact ctagcgaatc ctataaatat atgtcacaga
     5821 atgctttgat gggacttgat gatgttatca aagaaaatga tattgacatg ggaaacttta
     5881 ctgaaggaat tgctgatttc tatacgcttg aagacgaact atacgcaatc cctaaagatg
     5941 cttctgtagt ggggctatgg tacaacaaag aattatttga taatgcaggt gtcgattatc
     6001 cagatgaaac atggacatgg gaaacactca gacaagctgc aattgattta acagatgaat
     6061 caaaaggcat ctatggtttt gcatcacata ttggaacaga gactggctac tggccattta
     6121 tttatcaaaa tgggggagaa gtatttgccg atgacaatac aaaatcaggt ttgaataccc
     6181 cagaagcaca agaagcttta gctttttatc gagatattgc acttgttgat catgcttcac
     6241 caacaattac agaattacaa gatcctgata agttaagtcg ttttcaatca ggtagattag
     6301 caatgatgat tgaaggaaac tggtttgcac cgggattttt gaaaaatgac tatctagttg
     6361 aaaatgttgc ttctgccgtg ttacctaagg gaaaagttaa tgcaacaatt acaaatggac
     6421 ttggttgggc tgccagtgcg agtacaaaat acccagaaga agtgaaaaaa ctaatggcat
     6481 ttttagctag tgaggacgcc aataaaatcc aagcggaaac gggggcatca atcccagcac
     6541 taaaaggctt tgaagacgag tgggctgctt cttcagatac aattgatttg agtgcttttg
     6601 gaaaaatgtt ggagtatgga gtccctcgtc catacaataa agatggatta cgagctgaac
     6661 aagcagaagt tcaaattatt aaccgtattc tgtctggtga agtaactgtt gaagatggaa
     6721 ccgctcaaat tgaaaaagaa gtcaatgcga tattgagcgg aaattaaatt agttaattat
     6781 acaatataca ggcgaaagga ttctatttcg tctgtatatt aatggatgaa taccaaaaag
     6841 ggggatatta tgaaaaatcg aatttttgga aaatatcgta aagatcaagt gatatggggc
     6901 tatattttta tttttccaac ggcattagga ttaattttat taaatatatg gccagcaatt
     6961 caaacagcaa ttttaagttt tcaaaaaacg gtagggttcg gtgatactga atgggtcgga
     7021 ttaagaaatt accaaaaaat gtttgcagac ggtgaagttt ttcagtcttt attaaacaca
     7081 ttaatttatg cagtggtttc gatacctagt attgttgtat tatccttgtt ggtggcagtt
     7141 ttaatgaata gaaaagttaa aggattgtcg atttatcgga caatttattt tctaccagtc
     7201 gttgctgcac catccgcagt cgcaatgatt tggcgctgga tgttcaataa tgattatgga
     7261 ctgattaata acatgttggc taaaataggt ctcgatggac cagcatggtt aacggatcca
     7321 tcgattgcga tttattctat tatcattgta ggagtttgga gtgctattgg ttataacatg
     7381 gtgttattac tagccggctt ataagaaatt cctaaagatt attatgaagc agcggaaatt
     7441 gatggtgcta gtccaatcca gcagtttttc aacattacgt taccattagt tacaccaaca
     7501 ttattttttg tagttgtaac aacagttatc aatgctttcc aagtatttga tgttattttt
     7561 atgatgatta ctcctaatag taccgccatg tttaaatcac aatcattagc ctatttattc
     7621 tataaacatt catttatttt aaatgacaag ggatatggtt cggcaatcgt tatgttcctt
     7681 ttagtgatta tattaattat tactgcaatt caaatgaagt tgcagaaaaa gtgggtaaac
     7741 tattaaggag gctatcaaat tatgaaaaat aaattttttt caaatgcgcc aatccatatc
     7801 atgcttattt taggtggtat tgcaatggca ttacctttta tttggatgat tttaacttca
     7861 gggaaaacgt tggttgaatc tacccaaatt ccacctacaa ttttccctga gaaatttcag
     7921 ctaagtaact atcaacaagt acttgattta ttaccattca tggcttttta tatcaataca
     7981 tttttaatgg ttgttggacg agtgattggc tcggttttat tttcagcaat ggctgcatat
     8041 gcttgtgcgc gtctaagttt tccagggaaa aatatccttt tcagcttagt acttattcaa
     8101 atgatgattc ccggttccat ttttattact ccacagtatt tattagcaca aaaattgggt
     8161 attttaaata caattccagc attaatatta ccaggggtag ttagtgcgtt tggtacgttt
     8221 ttattaagac aatttttctt aggtttacct gatgaattgg aggaagcggc ctatttagaa
     8281 ggtgccaata tttggacgat ctttttcaaa attatgttgc cgcttgctaa atcaggattg
     8341 gttgcacttg gaatattcac agcgttgttt gcatttaaag atttaatgtg gccattgatt
     8401 gtcaatactt caattgataa gatgccttta gctccgggat tagcaaactt acaaggacaa
     8461 tatgccacga acttcccaca attaatggca ggttcgttat tagcaatttg gccaatagtt
     8521 gtgatattca tcgttttcca aagaaaattc attgaaggaa ttgctacaac aggtagtaaa
     8581 ttataaaact attaattagt gattggagag taagtacatg acaaaaatta catttattgg
     8641 tgcaggaagt actatttttg caaaaaacgt attaggcgat gcgatgttaa ctccaagtct
     8701 acaagatgca catattgcat tgtacgatat tgatgaaaaa cgactaaaag aatcaaattt
     8761 aatgcttaaa aatattaata aaaattcgaa tgaaaatcgt gctgatattg tttcttatta
     8821 tgatcgcaaa gaagcgttaa gagaggctga ttttgttatc aatgctattc aagtgggtgg
     8881 ttataaacct tcaacagtga ttgattttga tattcctaaa aaatatgggt tgcgacaaac
     8941 tattggtgac accatgggga ttggtgggat ttttagaacc ctacggacac taccagtaat
     9001 gttagacttt gctaaagaaa tggaagaagt ttgtccaaat gcttggttat tgaactatac
     9061 aaatccgatg gcgatgttaa ccatgggtgt tttaaaagcg acgagtatta agactgttgg
     9121 tttatgtcat agcgttcaag tatgcgtacc agaattgttt gagcatttag gaatagccga
     9181 tcaatatgac ttaaatgact ttcaatggaa aattgcaggc attaatcata tggcattctt
     9241 gttggaaatt acaaaaaatg gggaagattt ctatccaaca attcgtcagt tagctgcaga
     9301 aaaaccaaat ccacatccag attctgtacg ttttgaattg ttgaaacgtt ttggttacta
     9361 tattacagaa tcaagtgaac acaatgcaga ataccatcca tactttatta aatcaaaata
     9421 cccagaattg attgacgaat tagggattcc gttggatgaa tacttacgtc gttgtgaaca
     9481 acaaattaat gattgggaaa gcatgcgaga ggacattgta aataatgggg agttaacaca
     9541 tacgagaagc cgtgaatatg cttcatatat catggattct attacgacag gaactccgtc
     9601 agtgattgca ggaaatgtct taaataaagg cttaatcaca aacttaccga cagatgtttg
     9661 tgtggaagta ccttgtttag tcgataaaaa tggcgtgcaa ccaacctatg tcggtgaatt
     9721 gccaacacaa ttagctgctt taaatcggac gaatatcaat gttcaagaac tgacagtaga
     9781 agccgcttta acactagata aatcaaaaat ttatcaagca gcgtatttag atccacatct
     9841 atcatctgaa ttgtctttaa cagatatcaa aaatttagtg gatgatttaa tcgaagcaca
     9901 tggggactat ttacctgaat ataattaaaa agaagctgaa agagtaagga attttcaatt
     9961 ccttactctt ttcttagtta aatttagtaa aaatattgac aaaacactta gtaaacagtt
    10021 acattagtgt taacaaaaag aaaacgcatt caaattgtat aaggaggagc ataaaatgaa
    10081 gtggtctaaa aaaattattt tagcttgtat agcactagga gcaacgatct ctttgactgc
    10141 ttgtggaaaa aatcaggaag atgcaagcgg taaggtagaa atagagtttt ttaatcaaaa
    10201 aaaagaaatg actcaaacga ttcaggagat tgctaaagat tttgaagcaa aaaaccctga
    10261 tattcatgta aaagttgtgg atgtaccgaa tgctggtgaa gtaattaaaa cacgtatgct
    10321 tgcaggcgat gtaccagatg tcattaatct ttatcctcaa agtatagaat tgaaagaatg
    10381 ggcgaaagct ggttatttag aggacttaac cgaagaaccg tatttagaga atatcaaaaa
    10441 tggatatgca caacgttttg ctattgagga tcgtgtatat agcataccat taaccgctaa
    10501 tgtctatggt ttttactata acaaaactgc ttttgaggaa atggggattc aagcaccaga
    10561 aacttgggca gaatttgaaa aaatagtagc tgagattaaa gatcaaaaca aagtaccgtt
    10621 tgcgattgca ggagcagaag gctggacttt aaatggttat caccaactag ctatagcgac
    10681 tgctgctggt ggtgaagagg aagcgaacaa tgtttggcgt ttctcagaag ttaatggaat
    10741 taatgctgaa agcaaagaaa tgcaaaaaga ttttaatcga ttagatttgt taagagaacc
    10801 aaaagctttg caaaacaact ggcaaggtgc cggttataac gatacagtag ttacttttac
    10861 aaaaggtgat gcgttgatta tgccgaatgg ttcatgggca atgccgatga ttcattcaca
    10921 aaatccagat tttgaagtag gaacttttcc ttttccagca gatgaagcag gtcaaagtct
    10981 aacgattggc gcaggagacc tagccttatc agtttctgca acttctcaac ataaagaaga
    11041 agccaaacgt tttgtagaat atatgacaac acctgaagcg atgcagaaat attatgatgt
    11101 agatggtgca ccgtgtgctg ttgagggcgt tatcgagaat actcaggatt caccattaag
    11161 tggtttaact gaacttgctt ttactgatcg tcatttagtt tggttagcca aagattggaa
    11221 tagtgaaaat gatttttata ccttaacaac aaattattta cacaatggca atcaaaaagc
    11281 catgattaac gcattaaatg ctttctttaa tccaatgaaa gctgacggaa ggtaggaaaa
    11341 taaaatgaaa ataaaagaaa ttttaaatcg ttactgggca atcatttttg tagcaattcc
    11401 aattattttg cagatgattt ttttctactt tccaatggtt caaggtgctt tttacagttt
    11461 aactaattgg actggtctga cttataattt tgattttgtg ggtttgaaca attataagat
    11521 tcttttttct gatccgaagt ttatcaaatc gattggcttc actttaattg tgacgctttg
    11581 catgattgct ggacaaattg gttttggtat tttgattgcg cgggcgttga acgcaaatat
    11641 taaagggaaa acattcttta gagcatggtt cttttttcca gcggtcttat cgggcgtggt
    11701 tgtttcttta gtatttaaac aaatttttaa ctatggttta ccaaaaattg gtagtatgtt
    11761 aggcattgaa tttttacaaa ccagtctttt aggaacaaca tggggagcga tttttgcagt
    11821 aatttttgtt ttactttggc aaggaattgc catgccagtc atcattttct tagcgggttt
    11881 tcaaagtatt ccacaagaaa ttattgaagc tgctagaatg gatggggcga atgataagca
    11941 aattttttgg aatattgaaa tcacctactt attaccaagt atttcaatgg tatttattat
    12001 ggcattaaaa ggtggcttaa cagcatttga tcaaattttt gctttaactg gtggtggacc
    12061 aagcgatgcc acaacatctt tggggttact tgtttataat tatgctttta aaaacaatca
    12121 gtttggttat gcgaatgcta ttgctttagt cttatttatc ttaattggga ttgtttcact
    12181 ggcacaatta aaattatcga aaaaatttga aatctaggag gtttcccaat gaaaacatca
    12241 aaagaaaaaa atatttctaa atacttgctt ttattattag ggggcttcat gattttagtt
    12301 cccttagttt caacgctatt cggttcattt aaaacaacac ccgatattat gaataatttc
    12361 tttcgttttc ctaatccagc gactttggaa aatttccgaa gattgttagc ggatggaatt
    12421 ggtcattact tttggaattc ggcaatcatc acaatcattg cagtagttgc tgtgacagca
    12481 attattccta tggctgctta ttcactagca agaaaaatga ctcgtgggaa agcatacgga
    12541 ttgatgtatt tatttttaat tttaggaatt tttgttcctt tccaagtaat tatgattccg
    12601 attaccgtta tgatgagtaa aattggttta gcaaatatgt ggggattaat cttactctat
    12661 ctcacttatg ccgtaccaca gacactattt ttatatgttg gttttattaa actaaatgtg
    12721 cctatcagtc tggatgaggc tgctgaaatt gatggggcta gtcactttac aacttatttc
    12781 aaaattgttt tcccaatgtt aaaaccaatg catgcgacga ctattattct taatgctcta
    12841 tggttttgga atgatttcat gctaccatta ttaattttaa atcgtgattc taatatgtgg
    12901 acactaccat tgtttcaata taattatacg ggacaatatt ttaacgacta tggtccaagt
    12961 tttgcttcct atacagtagg aattgtcacg attacactag tttatcttgc tttccaaaga
    13021 agtattatag ctggaatgag caatggagca gtgaaataac caataaatta ttctcattat
    13081 aaaggagttc ataaacttat gattatattt gacaatcaca cacaaacatt tcatttacaa
    13141 aataaaaaaa ttagctatct attaggaatc gaagaaaata attatttaac gcatctttat
    13201 tttggaaaaa agattacgca ctattctggt gggtatcgct acccccgaac agatcgttcc
    13261 ttctcaccca acaatgcaaa tgcgcaagat cgtttattat ctttagacac attaccatta
    13321 gaatttccag gatatggaca tggtgatttt agagaaccgg cgcataatat taaattagga
    13381 aacggtagtc gtgtaaatga ttttcgttat gatagctacg aaattcaaaa aggaaagccc
    13441 agcttagcgg gattgccagc cttatatacg gaagatgatg atgaagctga aacgctgatt
    13501 attcaattga aagacgcgat tagtggattg actttacagt tgttctacac aatttatcaa
    13561 aatcacagtg taattattcg ttctacgcaa ttagtaaata atagtgatga aacagtggag
    13621 attaaccgct tagctagtca atcaattgat tttccaaatc gtccactcga gttaattcat
    13681 ttaaatggta cctgggcaag agaacgtcaa atgactagag aaaaaattca tacagggata
    13741 aaagtattag atagtaaacg cggatcaagc agccatcaac aaaatccgtt tgttgcttta
    13801 gtagatgcca aaacagatga atttcaagga gaggcatatg gatttaattt agtttatagc
    13861 ggtaatcacg aaacggtgat tcaaaaagat ccatttgacc aaacacgggt cattatggga
    13921 attaattcat ttaattttgg ttggcaacta aaaactggcg actcatttca aacgccagaa
    13981 gtcgtgatgg tttatagtaa tgaaggtctg aatacgttat cacagacgta tcatgaattg
    14041 tataatcacc gtttaattcg tggaaactat cgtttaaaag aacgtcccac gttaatcaac
    14101 aactgggaag caacgtattt tgattttaac gaagaaaaaa tcatgaatat tgtcgatgat
    14161 gcgaagaact tgggcattga aatgtttgtt ttagatgatg gttggtttgg cggtcgtgaa
    14221 aatgacttca cttcattagg tgattgggtt gaaacagaag gcaaacttgc ttgtggtttg
    14281 gaaaatttag ccaagcaagt gcatgataac gggatgaaat ttgggatttg gtttgaaccg
    14341 gaaatgattt ctgaagatag tgaactattt cgagcacatc cagattgggc attgggtatt
    14401 ccaggtcgtg accgctcaat tagtcgaaat caatatgttc ttgatttttc aagacaagat
    14461 gttcgtgata atatctatca acaaatgacg gcgatttttg atcgtgtacc gattgattat
    14521 gtgaagtggg atatgaatcg aaatatgaca gaagtctatt ctgtcttgtt ggactcaacg
    14581 atgcaaggtg aagtggctca tcgttatatg ttagggttat atgaattttt agaaaagtta
    14641 acaacgaatt atccaactat tttatttgaa agttgttcgg gtggaggcgg tcgttttgat
    14701 gcaggtttct tgtattacat gccacaaact tggacgagcg acaataccga tgccgtggct
    14761 cgtctgaaaa ttcaatatgg aaccagctta gtgtatccaa tttcaagtat gggtgcgcat
    14821 gtttcagcag tcccaaatca tcaaacagga agagaaacga gtttaactat tcgtggcaat
    14881 gctgcgatgg ctggtgtttt tggttatgag ctagatctaa cgcaattaac ttctgaagaa
    14941 aaagcagaaa ttaaagaaca agttgccttt tataaaaagc atcgccaact tttgcaatat
    15001 ggaacttttt atcgcttgga aagtccgttt gaaggcaatg atactgcatg gatgtttgtc
    15061 tcaaaagacc aaacagaagc catggtcttt tatttccgag tattagcaga agctgcgcat
    15121 ccattagtca cattgaaatt agcaggatta gcagaagaag caacttatca aatggaagat
    15181 gtaacatttg ctggcgatga attgatgaat ctcggttttt atattgactc agaactacat
    15241 ggtgattatg cgacacaacg cttttatttg aaacagatat agtagcttga atttacaata
    15301 taagtgcaac accaaaagag actcattcca aacgaagaaa atatgtttta tttacacgca
    15361 tttactaaaa agcagcctga tttgaattgg gaaaatgaag aagtacgtga agaaatctat
    15421 gcaatgatta actattggtt agataaagga ctaggcggat ttagaatcga tgcgatatta
    15481 aacatcaaaa aaaaactaga gtatggcatc tttgaatctg atggagaaga tgggttagcc
    15541 ttcattgggc cctggattct taatcagtcg ggcattgaag tgtggttaaa agaaatgaga
    15601 gagcggacat ttaaaccaca taatagtatg acggtagcag aagccgatgt tcctaatgaa
    15661 cgtttagatg aatatattgg tgaagatggc tattattcga tggtttttga ttttagttat
    15721 acggatattg atgtacctga aactggtgaa tggtttaaag atagccaatg gacatggacg
    15781 gatatgcgta ccaatatttt tacgaatcaa ttagttacgc aagataaagg ttggggcgcc
    15841 ttatatttag aaaaccacga tcaaccacgt tcaattaata aatatattcc agaagaatgg
    15901 atcaatgatt atagtaaaaa aatgttggcg acgcttttta tgttactaag aggtacccct
    15961 tttatttatc aaggacaaga actaggaatg accaacatag aaatggcatc attagaagat
    16021 tttgatgatg tagctacgca ttctcagtat aaacgggcat tagaatatgg attggcacca
    16081 gagcaagcat tgaaagctgt caataaacga agtcgggata attctcgcac acctatgcaa
    16141 tggaatacgc aaaaaaatgc cagattctca acttctgata atgtatggtt aaaagtaaat
    16201 cctaattatc cagaactcaa tgcagaagct cagttaacca atcaacagtc ggtattcaat
    16261 tattatcatc aattaattaa attaagaaaa aatagtgtat acaaagacgt gctaatctac
    16321 ggacaatttc taccagtgaa agataaaaat gaacatattt tgctttatga acgccaatta
    16381 gaagataaaa tcgtattagt tatcttaaac gttacagcaa cagaacaaga atattatgta
    16441 gatgaacagt accaacaagt attaatcaat aattatgaag atgttttatt agatcaaagt
    16501 cgaaaattgc gtttacgacc gtttgaaagt attgtattag ctaattatgg aggacaatta
    16561 tgaaaattaa aaacgaagca atgctcatca cctatgcaga tagtttagga aaaaacatgg
    16621 gcgaattaaa acaagtctta gacaaacatt tacaaggtgt tattggtggt gtacatctgt
    16681 tgcctttttt tccatcgaca ggtgatcgag gctttgcgcc gagtgattat acgacagttg
    16741 atccagctct tggaacatgg gaagatgtgg aagcattagg tgaagattat tatttgatgt
    16801 ttgattttat gattaatcac atttctcgtg aatcaaaatt tttccaagat ttcaaaaaaa
    16861 accatgagca gtcaccatac aaagaaatgt ttatccgtat tcatgaattt ttcccagaaa
    16921 atcgaccaac acaagaagat attgatttaa tctacaagcg taaagacaaa gcgccgtttc
    16981 aaacggtaga atttgcggat ggtacgacag aagaggtttg gaataccttt ggggaagaac
    17041 aaattgattt agatgtgacc aaagaagttg tgaaagaatt tattcgagaa accatcaaag
    17101 atatggcgaa ccatggctgt tctttaattc gattagatgc gtttgcttat gcgattaaaa
    17161 aattagatac caatgatttc ttcgtggaac cagatatttg ggaactttta gacgaagtgc
    17221 gtcaagaagc agcgaaatat gacgtcgaat tattaccaga aatccatgag cattattcca
    17281 ttcaaatgaa aattgccaat catgattatt atatctatga cttcgccttg ccaatggtca
    17341 ctctgtattc actttatagt ggaaaatcag aacgtttagc caattggttg aaaatgagtc
    17401 cgatgaagca gtttacaaca ttggatacgc atgatggaat tggtgttgtg gacgctcgtg
    17461 atttattaac agatgaagaa ttggattaca cctctgagga attgtataaa gtcggggcga
    17521 atgtgaagaa ggtctattca agtgccaatt acaataattt agatatttat caaattaaca
    17581 gcacctatta tagtgcatta ggcgataatg accgtagcta tctgttagca cgtgcgattc
    17641 aagtatttgc gccagggatt ccacaaattt attacgttgg gttattagca ggaaaaaatg
    17701 atattgaact gttggaagca acgaaagaag ggcgtaatat taatcgtcat tactatgatt
    17761 tagccgaaat tgaagaagaa gtgcaacggc cagtgattca acaattattt gcacttttaa
    17821 aatttagaaa tgaatctgcg gcgtttgatt tggatggaac gattgaagta gcaacgccat
    17881 cagaatcaga aatagtgatt acacgtaaaa ataaaaacgg tagtgatagt gcaacattaa
    17941 cggctaactt ggatacgaaa gaatttatca ttgagtctaa agatataaaa attttcacta
    18001 gtgttgcatt aaaattttaa cctattccat agaacaaaaa atcctttata ctagcacttg
    18061 gatgacttat ccaagaccaa taaaaaggac tcatacatgg ataagtataa aacaaataca
    18121 gcatttaata aatggttttc tgccataaaa ttaggaaaac tttctgtcaa ctcccaaaaa
    18181 tgtatttttg attacaattc gtatcgtaaa aaactgtctt ttgaagctgt gctcaagctc
    18241 tttctctacg cagttaatga agaaaaagaa agtttgcgtg atttgagtac agctcttatc
    18301 gaccgatcgc tccaactaga aaccgatgtg tcatcgatta gtcataccca attgtctcga
    18361 gcatttaatg cacttgaacc taaagtgtta gaagaaattt ttcaacaact gttagaaaaa
    18421 gttcaacatc aaatcaaacc aaccaagcga aatcatctgt atctgattga ttccagtacg
    18481 ttttcgctta gtttaaaacg tcataagtgg gcgactttcc gtcaaacaaa atcaggtgtc
    18541 aagttacact taaactattg ttatatggac gatactacga tgtatccaac tgattttacc
    18601 ttaacaaatg cccatgaaca tgatctcaat caactaccag tattggtgaa tcaaccagag
    18661 gcaacttacg tgtttgatcg tgggtattag gactttgaaa agatggatca gatgcattgg
    18721 gatggctatt tcttcgtcac tcggattaaa aaaaacacca aagttcacgt gatagatact
    18781 ctggaaacat cgccagaaac agaaattcta cgagacgagc tggttcgttt aggatctaaa
    18841 acctatctaa ccgccaattt ccgactagtc actgttcaag ataagaacgg acgtgtgttt
    18901 caatttatca ccaatcgaat ggacgtctct tcaaaagaaa tctctgatat gtatcacgct
    18961 cgttggcaaa tcgaattgtt tttcaagcac atcaaacaac acatgaccat taaaaccttc
    19021 ttttctcaaa gtgaaaaagg cgtgcagaat caattgattt taacgatgat ttccgctctc
    19081 ctcacgtttc tcatcaaatt ggagacgaaa acggaaaaat cagtgttcca aatcaaacga
    19141 ttctttcgtt atttactgtt tcagcccttt gaatgttgtc tagaaaaatt aattcccaca
    19201 tgagaaaaaa gaaatttggc tcgttacttt tggttttcag ttgaataaca gataaaaaat
    19261 tttctggaaa aagtcatcct ctgcgtcagc acagtttttt ttgacttatt ttcagaggaa
    19321 atagttttca gaatattcta aaatgtttta tgcaacacta gtgatatttt ttagaattag
    19381 atttctaatg tgcatttttt gagtattaag gagcaagtat acaaagattt ataaagctga
    19441 ttaaatttga ataaaaatat gcaggaacgc tactattttt agtagtgttc ttttttatat
    19501 ttgaataaaa aatgtggtgg ttaaatggtg gttataacaa aaaatgaatt aatgaaatta
    19561 ggttatggga agtatcagtc tgagagaatc atacgacagg caaaagcaat tatggtttca
    19621 aaaagctata cctattacga aaatagaaga ttaggactag tacctgttga atctgtagaa
    19681 gaagttttag gtatgaagat agaggtagag gaggattttc agaatcatgg ttaattttaa
    19741 aaaagataaa aatgggaaat atcattttac tgctagtttg ggattcgaca aacgaacagg
    19801 gaaaagagta caaaaaaacg tagaggggtt aaaacagtta aagaagaaaa aaagcgtata
    19861 ccgaacttgt ttctaattat ggaaaagaag cagaaacttc tcaaagtact atgttgtttg
    19921 aggagttttt ttataaatat tatttgtcaa attaagcgag acaaaacttt ccatttacgt
    19981 aactcaaaaa aacttctaat ggtgtttggt aatttagtga ttttctaggg atatggtttc
    20041 ttttggacgc aacggatgag atgaatcctt gattgacttg gttgaagtcc atttcttttg
    20101 gtagtccatc ttttcggagg agtccgtttg aatgttcatt taatccccgt tgggaaggcg
    20161 ttcctgggtc tgcgaaataa atatcaatat cattcgtatt actaatactt ttccaattag
    20221 agaattcttt tccacaatca aaggtaattg atttgaaaag atttttgggt acggattgaa
    20281 gccattcatt aatcgaattt tcaatatcaa ctgccttacg gccttctggt ttcaaggcga
    20341 taatggcttt tgaaagtcgc tcaacgagtg tgatgacggc acttttatgg tggatgccga
    20401 caatcgt
//
LOCUS       DAZKOW010000051        20360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000051 DAZKOW010000000
VERSION     DAZKOW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.051"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            291..1430
                     /locus_tag="ACG912_001707"
     CDS             291..1430
                     /locus_tag="ACG912_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFQ0513025.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1497..2072)
                     /locus_tag="ACG912_001708"
     CDS             complement(1497..2072)
                     /locus_tag="ACG912_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0513026.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2077..3051)
                     /locus_tag="ACG912_001709"
     CDS             complement(2077..3051)
                     /locus_tag="ACG912_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFQ0513027.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3075..3119)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3334..4302
                     /locus_tag="ACG912_001710"
     CDS             3334..4302
                     /locus_tag="ACG912_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0513028.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4415..4693
                     /locus_tag="ACG912_001711"
     CDS             4415..4693
                     /locus_tag="ACG912_001711"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0513029.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4811..5047
                     /gene="secG"
                     /locus_tag="ACG912_001712"
     CDS             4811..5047
                     /gene="secG"
                     /locus_tag="ACG912_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFQ0513030.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5150..5905
                     /locus_tag="ACG912_001713"
     CDS             5150..5905
                     /locus_tag="ACG912_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0513031.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5908..8265
                     /gene="rnr"
                     /locus_tag="ACG912_001714"
     CDS             5908..8265
                     /gene="rnr"
                     /locus_tag="ACG912_001714"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFQ0513032.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8287..8751
                     /gene="smpB"
                     /locus_tag="ACG912_001715"
     CDS             8287..8751
                     /gene="smpB"
                     /locus_tag="ACG912_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFQ0513033.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9053..9772
                     /gene="atpB"
                     /locus_tag="ACG912_001716"
     CDS             9053..9772
                     /gene="atpB"
                     /locus_tag="ACG912_001716"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFQ0513034.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9825..10040
                     /gene="atpE"
                     /locus_tag="ACG912_001717"
     CDS             9825..10040
                     /gene="atpE"
                     /locus_tag="ACG912_001717"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFQ0513035.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10131..10655
                     /gene="atpF"
                     /locus_tag="ACG912_001718"
     CDS             10131..10655
                     /gene="atpF"
                     /locus_tag="ACG912_001718"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFQ0513036.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10642..11184
                     /locus_tag="ACG912_001719"
     CDS             10642..11184
                     /locus_tag="ACG912_001719"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFQ0513037.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11207..12763
                     /gene="atpA"
                     /locus_tag="ACG912_001720"
     CDS             11207..12763
                     /gene="atpA"
                     /locus_tag="ACG912_001720"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFQ0513038.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12777..13679
                     /locus_tag="ACG912_001721"
     CDS             12777..13679
                     /locus_tag="ACG912_001721"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFQ0513039.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13702..15108
                     /gene="atpD"
                     /locus_tag="ACG912_001722"
     CDS             13702..15108
                     /gene="atpD"
                     /locus_tag="ACG912_001722"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFQ0513040.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15122..15544
                     /locus_tag="ACG912_001723"
     CDS             15122..15544
                     /locus_tag="ACG912_001723"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFQ0513041.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16013..16243
                     /locus_tag="ACG912_001724"
     CDS             16013..16243
                     /locus_tag="ACG912_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFQ0513042.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16340..17641
                     /gene="murA"
                     /locus_tag="ACG912_001725"
     CDS             16340..17641
                     /gene="murA"
                     /locus_tag="ACG912_001725"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFQ0513043.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17654..17830
                     /locus_tag="ACG912_001726"
     CDS             17654..17830
                     /locus_tag="ACG912_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0513044.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17965..18261
                     /gene="yidD"
                     /locus_tag="ACG912_001727"
     CDS             17965..18261
                     /gene="yidD"
                     /locus_tag="ACG912_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFQ0513045.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18325..18708
                     /locus_tag="ACG912_001728"
     CDS             18325..18708
                     /locus_tag="ACG912_001728"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0513046.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18877..19761
                     /locus_tag="ACG912_001729"
     CDS             18877..19761
                     /locus_tag="ACG912_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0513047.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19875..20111
                     /locus_tag="ACG912_001730"
     CDS             19875..20111
                     /locus_tag="ACG912_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFQ0513048.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20199..20279
                     /locus_tag="ACG912_001731"
     tRNA            20199..20279
                     /locus_tag="ACG912_001731"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20233..20235,aa:Tyr,seq:gta)
     gene            20286..20357
                     /locus_tag="ACG912_001732"
     tRNA            20286..20357
                     /locus_tag="ACG912_001732"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20318..20320,aa:Gln,seq:ttg)
BASE COUNT         6759 a         3566 c         4425 g         5610 t
ORIGIN      
        1 ttttttgcaa ctagcttctg tatatgatgt ttacaaagaa tggtacaaga aaagatcatc
       61 cttgaaaaag gaaagctaac aataactagc atcatataag agcttttaag gatagaacag
      121 tagaatagga atctatgggc aagtcctctc gtataaataa aataggaggg aagtagcagc
      181 atatttttca taaagtggct ctctttcttc atttttactt tggttgctcc tatttgaaaa
      241 ttcgagcaca ctcaaataca ataaaaagaa atggcaaggg agaggattag atgaaaaaag
      301 aagaaaaaat cgcgatttta caagagatta tccgaattaa atcagtgaat ggaaacgaag
      361 gtgaagtagc agcctatttg aataagcttt tggcaaggca tgacataaca ggagagattg
      421 tttcttacag agatggtaga gataatttga ttgctcgtta ccaaaagggc caatcaggaa
      481 aagtgttagg cctatctgga catatggatg tcgtggcggc tggtgatgaa tcgtcttgga
      541 cctatgcacc ctttgcagca gaaatccatg ggaaccgttt gtacggacgt ggtgctacag
      601 atatgaagag tggcttggca gcaatggtga ttgccatgat cgaactgaaa gagtcaggaa
      661 aaccatttaa cggaacagtc aaattattag ccactgtcgg agaagaagtg ggtgaactag
      721 gtggggaaca gctcacgaaa gcaggctatg tagatgactt agatgccttg atcatcggag
      781 agccaaccaa ttacagtttg atgtatacgc acatgggatc aatcaactat acagtcacct
      841 cgcacggaaa agaagcgcat agctctatgc cagaccaagg ttataatgca atcaatcacc
      901 taaatgaatt cattacgaaa gcaaatgctg aaatgaatca cctcgctgaa acaatcgaga
      961 atcctgtcct aggaaaaaca atccataatg ttacattgat cagtggcggg aatcaagtca
     1021 acagcatccc aagccatgcg cagctccaag ggaacattcg atcgattcca gaatacccaa
     1081 atgataaaat cattgcattg ctgcaatcaa tcgtcaatga attgaatcaa gagacagact
     1141 atcatttgga attgatgatc gattacaaca aaatacctgt caaagctgat ccagattctc
     1201 ctttgattca ttccattcag caacaattca gccagccatt acctttagtt ggagcggcag
     1261 caacaacgga tgcagccgaa tttacgaaag ctaaccattc cttcgacttt gtcgtctttg
     1321 ggcctggtgt cgttactttg cctcatcaag tagatgagta tgttgaaatc gataattatt
     1381 tggatatgat cgaaaaatat caaggaatta ttttatccta cttagcgtaa gattttttta
     1441 ataaatgaaa aaacagagcc agattagcat tattttgcta atccagctct gtttattcag
     1501 cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac taatgaaaca
     1561 acgacgatag acaacaagcg atcaaggtag tctgtaccca cctgtaccaa gatcacactt
     1621 gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga tcctgaggag
     1681 gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc aggtaccgac
     1741 aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag atatcttggc
     1801 aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata cagagaaaaa
     1861 atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat tccaggtaca
     1921 ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat tggcaaacga
     1981 agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc catcactgta
     2041 agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta acctttccaa
     2101 atctttcata atcgtttctt cttgtgcatt taaaagaacg gttaaaaact ttcggaaaaa
     2161 aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc aaaattcata
     2221 acgatcaacc aatgtttgtc cgcgacttat tccttgagtt tctacagcag tgaagctttt
     2281 aaagccttca cagatggact catcgataaa ataagcaaca gctaatggat cattgatgac
     2341 acatcctaag attctttctt gtttccagtg aaagtcaaaa taaaaacgag tgatcgcttt
     2401 tagatactct ccctcttcag gattcatttg gcagcaatat tcaaggatag tcggtgtcaa
     2461 gacgatttct cttgtcacat ctaacccaac catttctatc ggttgtttga gcttttcgaa
     2521 tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg gtgaacagtt
     2581 accatggctt ttgtaagttc cgcccattga gacaaaacga tccatatgtt tcccaacaga
     2641 tgggctgttt ttcaaagcag aagcgatatt ggtcaaaggc cccaaagcaa tgactgatgt
     2701 atctgttttt tctttgaaat aatcagtcag aaaatcaact gcatggagcg attctgcctg
     2761 tttggctaag accatcggga aatttgtgtc ccctaatcca tccaacccat gagtgtcttg
     2821 cgcactaatg aaaggttgct tcaaaggttt ttccattcct tgataaacag ggatatctaa
     2881 acgatcacag cgctccaaac atttcagcac attctgcgta ccgatatcta cagggacatt
     2941 cccgcaaacg acggtaatag caactacttc gatatcaggg gatttgacag ctagtgataa
     3001 agctaaagtg tcgtcgatcc ctggatcgca atcaataatt actttacgca ttatttccaa
     3061 tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac cagtccaaga
     3121 cgagtgtctt gtttataatg cacgaaatta gtatactggc ttttattgtg aatgtaaatg
     3181 ctctcttaca agtaaatcta ttgagaagaa ctttaagtaa atgatacaat gaaaaagaaa
     3241 tggaatttac gtgtgccgaa gaaaaagaat cctattgatc ggtatattgt ataataaata
     3301 gattatagtt tggcagaaag cggcgggtgt acgatggtta ttttttattt cattcttatt
     3361 ctgatattta taggaatcag ttaccatttt ctttcttatt tttatgcaaa gaaattagta
     3421 agagcggggg tacagagagg ctatgactgg tatgaagaaa ctgggcaccg cttgatggat
     3481 cctgctgcag tcacgtcagt agaaaaaaag cgacaaaaat tagaagaaga gtgtgaaagc
     3541 ttctgggaac aaggaatacc gaaaacgatc aaaagttatg acgggttgaa actagctgga
     3601 cagatgttcc tccaaccaac tcagcagaat aaatgggtca tttgtgtaca tgattatcga
     3661 agtacaggga aaagagatat gtctcatata ggtaaaagat atgcagagaa aggattcaac
     3721 gttcttatac ccgatttacg tgcgcatgga gaaagtgaag gagaaatcat cggaatggga
     3781 tggctagacc ggctagattt aattgcatgg atccaactca tcttagatga acaaccagat
     3841 gcatccatta ttttgcacgg cagttcgatg ggcgcttcta ctattatgat ggcaagtggt
     3901 gaaaagcttc catctgcagt caaaggattt atcttagaca gcggatatgt ttctgtttat
     3961 gcggaatttc ggtatatgtt aagtaaaatc acggtctttc ctaaaaaaat gatcatgaga
     4021 tatgcgaatc actatgctca gaaatacgca ggttattctt taaagcaagc atcagctaca
     4081 agacagcttg gtagtaacca tttgcctttg ttagtcatcc atggtgaaag agatcatttt
     4141 gttcctacag aagcagcata cacgattcaa aacgctacgg caggggataa agcattactg
     4201 cttgttccag aagcagaaca tttggaagca tcgatgaaag atccgaaaac ctattggaca
     4261 gtgatttttt catttattga acaaagaata acgatggatt gattttctct tcctttttgt
     4321 aagcaacacg aggataaata attgtttata gaccatgaga ctgttaaaat aaggtaggaa
     4381 taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagagg aaaaaaatcg
     4441 tcttgcttta ttcaatgatg agcaacaaga aatcggagaa atgacatggt cggatgcggg
     4501 gccagacatc atgatcattg accatacatt tgtcgatcca gcatatcgtg gtcaaaaatt
     4561 agctgaaaag ctagtgtata ctggagtaga actagcaaga cgagagggga aaaagattat
     4621 cccttcatgc ccttacgcaa aaaaagagtt tgagaagaaa ccagagtatc atgacgtctt
     4681 acgccaatca taataataac agctgaaaga tttgcacttt cagctgtttt tccatttata
     4741 tagagttgtc acggagtttt atctatgata aagttaaaga gacgatatct tttgggaagg
     4801 aggctgctat atgtataatt taattttggg aatcgtgatc gttttatcga ttgttatggt
     4861 cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat ttaccggcgg
     4921 tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca tgcagcgatc
     4981 aactgcagtc atgggagctg tctggatgat cttattattt gtcctagcat ttttatcttc
     5041 gaagtagtcg aataagcctc tcgcacaacg gcgggcggct ttttttattg caaagagaac
     5101 gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg tgaaacgagt
     5161 gaaacaatta ccaaaacccc tttatgcaaa acatggaaaa cgagcagttt tgctgcttca
     5221 tgcgtactca ggaagtccaa atgatgtccg gatgctcgca cggtttttag aaaaagcaga
     5281 ttatacagtc tatgcgccgc tatttaaagg acatggaacg atggagccat atgatatctt
     5341 gcaagaaaaa gcagaaagct ggtgggcaga tactaaaaaa gctatccatt ttttacagtc
     5401 tgaacagttt tctgatattg ccgtattggg attatcgatg ggtggcatct ttgctgtacg
     5461 ggcactagaa gaagaatcag taattggcgg cggttttttc tgttcgcccc tgtctcctgt
     5521 agaaacaaac gtgcctgaaa attttgaaaa atatgtacgt caagtgctga agatagcagg
     5581 caaatcagaa aaagaaatca atgaaaaagc tgtgtcttac cgctcgttag ctgaacatca
     5641 attaatggat atacaagacc aagcagcaat cgcagaaagt agactatcag atatccaaca
     5701 gccaatattt ttagctcaag ctgggaaaga tgaaatgatc gacccaaatg gtgtatttga
     5761 aacggcacgc aaattgagcc gccaacgagt gacacttcaa tggtatcctg aaagcggtca
     5821 tgtgattaca gttggcacag caagacgcga attagaaaaa gacgtgttgg aatttttaga
     5881 aaaattacct tggaatgagg aataaatatg acaaaagaaa caataaaaga gaaaattctc
     5941 tttttcatgg aaagcagtcg caaacgtagt ttttctatgg aagaaatcgc tgaaggtcta
     6001 ggctttcaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca aatggaaaga
     6061 gaacagagta tcgttttcaa taaaaaagga aaagtcaaac ttcctttaaa accgatcttg
     6121 gtcgaaggga catttcgggc aaatgaacga ggatttggat ttgtaacgat tgatcccgaa
     6181 gaagatgata tctatatccc gaaagaagcg actggctttg caatggatgg ggataccgta
     6241 gcaattgata tcatgaagcg agcaaataca gatatggatc gcggagcaga gggaaaagta
     6301 gtggagattc gagaccgagc gactactcag ttagccggag aatttgttgc ttattcagac
     6361 gaagagatac aggaaacgga tctttatggt gtggtcatcc cgaaagatag aaagcttaac
     6421 caattcaaag tgtatgcagc ggcagaggga gttcgtccag ttgacggaag tattgtattg
     6481 atcgaactga cccattaccc tgaaaaaggg tatgcaacta gtttagaagg gataatcaag
     6541 caagtgattg gtcataagaa tgaccctggc atggatatcc tctcgattgt cgtggctaac
     6601 ggtattccta caaaatttcc tgatgatgtg ttatctgagg cagatgaagt tccagaccag
     6661 atcagcgaaa aggatattgt tggacgcaga gatttaagag atcagctcat cgttacgatt
     6721 gatggagaag atgcaaaaga tctagatgat gcagtgacgg tcaaaaaatt agaaaatggt
     6781 aattattttc ttggagtaca cattgcagat gtatcttact atgtgacgca aggcagtcaa
     6841 ctggacagag aagcatacga aagaggaaca agtgtctatt tgaccgatcg agtgattccg
     6901 atgatcccac aacgtttatc caatggtatc tgttcattga atccccatgt tccacgactc
     6961 accatgagtt gtgaaatgga gatcgatcca aatggacaag ttgtctctca tgagattttt
     7021 ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca aatcttagaa
     7081 gatcaagatg aagaaacgat gcaacgttat gctcagctcg tgcctatgtt ccaagaaatg
     7141 ggtgaattgc atcgaatcct tgaaatgatg cgaatcaaac gtggagcgat ttcttttgag
     7201 gatcgtgaag cgaagatcct tgttgatgct gaaggacacc caaatgatat ccagctaaga
     7261 agcagaggga taggtgaacg cttgatcgaa tcctttatgc ttgctgccaa cgaaacggta
     7321 gctgaacatt ataataaatt aaatcttccg tttatttatc gaatccatga acagccaaaa
     7381 gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct ggtccgagga
     7441 acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt agaagacaaa
     7501 ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc acgctattca
     7561 gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt tacatctcct
     7621 attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag tgaagataaa
     7681 agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga tcatagttct
     7741 aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa gaaagcagaa
     7801 ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt tgtgaaattc
     7861 ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa tgaattgaaa
     7921 caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag aactggtctg
     7981 acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc agatgaacga
     8041 gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg tccaaaagca
     8101 cgcaatggaa aatctggaaa acgagaagga aaacaagcag gcaaacgctc agaaaaaaat
     8161 ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa aaaagggaaa
     8221 aagccatttt acaaaggtgt caaaaagaag aaaaagaaaa aataaaatga gttggaggaa
     8281 acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc tcgccatgat
     8341 tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga gatcaagtcg
     8401 atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa cggggaagct
     8461 ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa tcatgatccg
     8521 ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga aaacgaattg
     8581 aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg ctatgccaag
     8641 gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga tttgaaacga
     8701 aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata atttcattat
     8761 cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt tagcatttaa
     8821 tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt gcatggggaa
     8881 aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa aatgttttga
     8941 attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga aaaaaccaga
     9001 tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg gattggatga
     9061 acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt gtataatggt
     9121 gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa atcttaagtt
     9181 gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc gaggaatcgt
     9241 aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat ttacattgtt
     9301 tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct tacctaacga
     9361 aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt tggcatttat
     9421 aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag gatatttcgt
     9481 aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg aagaatttac
     9541 aaacttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg aagttttgct
     9601 aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat tagcaattcc
     9661 attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag catttgtctt
     9721 tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat aatactcggt
     9781 taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat tatatcgcag
     9841 cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt caagttatct
     9901 ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc cgtacaacaa
     9961 tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt atcgccttga
    10021 ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc gtttgttact
    10081 gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct atgctgaatc
    10141 aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt gtcagcggct
    10201 catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc agcgatattt
    10261 tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa tctcgcatca
    10321 actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt tctgatgcag
    10381 ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat attttgaagg
    10441 atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc actgtagaac
    10501 gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt caaatcgcgg
    10561 aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc aatcaataca
    10621 ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag acgttacggg
    10681 aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta tcaagatctc
    10741 gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt aagtgatgtt
    10801 cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg ttatgatgga
    10861 atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga ccttttgttc
    10921 atgattgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt gggaagtgtc
    10981 acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa tgttgcaaaa
    11041 acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat tattggtggc
    11101 gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca attagaaaaa
    11161 atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg ccatcaaagc
    11221 agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg tgctttcagt
    11281 cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac atggtttaga
    11341 aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa tggcgcaaaa
    11401 cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca ttcgtgaagg
    11461 agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg ccttgatcgg
    11521 tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg ttactgacaa
    11581 agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg ttaacgaacc
    11641 aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg gacaacgtga
    11701 attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgattgata cgatcatcaa
    11761 ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag attctacagt
    11821 tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa tcgttgtgaa
    11881 tgccggtgcg tctcaaccag caccattact ttatatcgca ccatatgctg gtactgcaat
    11941 gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg atctttcaaa
    12001 acaagctgtt gcttatcgtg aactttcctt gctacttcgc cgtccaccag gtcgtgaagc
    12061 ttatcctggg gatgttttct acttgcactc acgtttatta gaacgtgcag caaaattaag
    12121 tgatgatcta ggcggcggtt caatgactgc tttgccattt gttgaaacac aagctggaga
    12181 tatctctgct tatatcccaa caaacgttat ctcaatcact gacggacaaa tcttcttaga
    12241 aagcgacttg ttctacgcgg gtactcgtcc agcggttgat gccggactat ctgtctctcg
    12301 tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt tacgtctaga
    12361 cttagcaagt tatcgtgaat tagaagcatt tacacaattc ggttctgatt tggatgcggc
    12421 aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac aaaaactgca
    12481 tgcaccgcta gctgttgaga aacaagtagt gattttgtat gccttgacac atggattctt
    12541 ggacagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg aatatctgga
    12601 taccaatcat tcagatattt ttgaaacaat ccgtacaaca aaagatcttc cagaagaaga
    12661 aacgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa aaggaaataa
    12721 ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg agatgaatgg
    12781 gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg agccaaatca
    12841 cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct tcttcacaga
    12901 agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact gctactcagt
    12961 taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc atgctgctca
    13021 gccgtcctgt gaagaaaacc ggttatatcg tcatcactgc agatggcggt ctggtcggtg
    13081 gatataacag ttcgatattg aaacaaacga tgtctatttt agaagaagac caccaatcac
    13141 ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt aaagcacgcg
    13201 gtatcaacct agcatatgag ctgcgcaatt tatctgatca gccaagcttt gatgaagtcc
    13261 gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat gaattatacg
    13321 tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa aaaatgctgc
    13381 caatctcaga cttagatcca gaagaagcaa cgactttcga acaagaatat atttttgaac
    13441 cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt ttgatctacg
    13501 gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca atgaaaacag
    13561 caactgataa tgctgctaat atcattgatg agctgacggt ttcttataac cgtgcaagac
    13621 aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca ctagaatagt
    13681 attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat cggtcccgtt
    13741 gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc attagttgtt
    13801 tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga attaggggac
    13861 ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg aatggaagtc
    13921 attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg tcgcgtattc
    13981 aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc ggaaagaagc
    14041 gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga aattttggaa
    14101 acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa agtcggacta
    14161 ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca taatatcgcc
    14221 caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg tgaaggtaat
    14281 gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat ggtgttcgga
    14341 caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt gacgattgcg
    14401 gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa cattttccgt
    14461 ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc tgcggttggt
    14521 tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac atctacgaaa
    14581 aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta cactgacccg
    14641 gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg taaattaaca
    14701 gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc tttagcacct
    14761 gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt attgcaacga
    14821 tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc cgatcaagaa
    14881 aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa ctttaacgtg
    14941 gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac agtgaaagga
    15001 ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt ccgaagtgtt
    15061 ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa aggggtgccc
    15121 catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct atgatcacca
    15181 tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc ctaaacatgc
    15241 accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag attcagatac
    15301 ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata atgttgtttc
    15361 aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg cggaacgtgc
    15421 aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata cgaacgaatt
    15481 gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat ctaagcactc
    15541 ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg ttatgacaga
    15601 agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa atttttgtga
    15661 aatctcagct tatttttcga agaagctact tctggaacac cgtatatcag aggttgtgaa
    15721 atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc atattttgcc
    15781 gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg aataagggac
    15841 tgcgacaaga cttttgtctc agtccctttt tttgcatatt tttagcattt atttcctaaa
    15901 aaatgtaagg ggattcctat atttttaagt atacttgttt ttccttttat acattttatg
    15961 gtattatcat tttattagtt attataaaac aatcgttaga aaggacgtca ttatgcaagt
    16021 atacggaatt gatgcggtgc ttcgaatcgt gagtcatttg gcgtttatct acttagcctt
    16081 ctggtcttta cgttcgttac gcattgaaac ttttttcaga ccaatgcata atacgcaaat
    16141 tagaatggca atcgtacttt tttcaatttt tttagggtac actgcaagta attttttctt
    16201 agaagttatc gctttatgcc gaaatctttt cgttagcctg taaattttaa aaaaaggtac
    16261 tattttaaaa gcaaggatgt tataatagaa aagattttga gtaagtgtat gctcattgga
    16321 attttttgga gggaacaaca tggaagagat catcgtaaga ggtggcaatc aattaaatgg
    16381 aacagtaaga attgaaggag ctaaaaatgc tgttttgcct attttagctg ctagtttgtt
    16441 agcagaagaa ggtattacga cattagacaa cgttccgatt ctttctgatg tcttcactat
    16501 gaatcaagtg attcgtcact taaacgtaga tgtagatttt gatgaacaaa agaatcaagt
    16561 aacaatcgac gcttcaagac aattagaaat cgaagcgcct tatgagtatg ttagtcaaat
    16621 gcgtgcttca atcgttgtga tgggtccgct acttgccaga aatggacatg caaaagtagc
    16681 tatgcctggt ggatgtgcga ttggtaaacg accaatcgat ttgcatttaa aaggatttca
    16741 agctttagga gcaaaaatta ttcaaaaaaa cggctatatc gaagcaattg cggatgaatt
    16801 gatcggtaac acgatttatt tagatttccc tagtgtgggt gctactcaaa atatcatgat
    16861 ggctgccgta aaagcaaaag gaacaaccat cattgaaaac gtagctcgcg aaccagaaat
    16921 cgttgattta gcaaacattt tgaataaaat gggcgctcaa gtctatggag ctggtactga
    16981 aacaatgcgt attgaaggcg tggatcattt acatgcagtc aaccactcaa tcgtacaaga
    17041 tcgtatcgaa gcaggtacct ttatggttgc tgctgcaatg acacaaggca atgtgctgat
    17101 tgcagatgct atctctgaac ataactgccc attgatttct aaattgatcg aaatgggtgc
    17161 agaaatcatt gaagaagaag gcggagtccg tgtcatcgga ccaaaacata tcttacctac
    17221 agatgtaaaa acgatgcctc atcctggttt ccctacagat atgcaagctc aaatgacagc
    17281 tatccaatta gttgcagaag gtacaagtgt ggtgactgaa acagtgtttg aaaatcgttt
    17341 ccaacattta gaagagatgc gccgtatgaa cgctcatgtc aaaatcgacg gaaatgtagc
    17401 aatcatggat ggaaaccatg aattgcaagg tgcagaagta tatgcaacag acttgcgggc
    17461 agcagcagca ttagttttag ctggcttgaa agcaaatgga atcacacgtg tgcgtaactt
    17521 gaattatcta gaccgtggat attataattt ccatatcaaa ctacagcaat taggtgcaga
    17581 cgtcgaacgt gtagacatgg atcaaacttc tgctgaaaaa acagcacaaa cgattgctta
    17641 aggaagtggc tgaatgagta accaacgata tatcttatta actttgataa agattttagt
    17701 ggtgatctta ttgcttatcc ttctttttgt ggccggaacg atgatcggat atggtgtcat
    17761 cggtggcggc aatccattta aagtattcca gccaagtttg tggattcata ttcgagactt
    17821 ttttcattga taaataagaa tcacagtggt tctagtcgat atgaagtgag ccatgaagca
    17881 gaacatgata aaagaaaaca gaagggaacg ttgacgttag tcgcgatccc tttttttagt
    17941 aaaagaaaag aaggaatcta attgatgaag aagatattga tttatttagt tagaggatat
    18001 cagcgcttta tttcgcctgc atttccacct agctgccgtt attacccaac ctgttccaat
    18061 tatatgataa aagcaattca aacccatggt gcgctaaaag ggacggtaat ggggactgca
    18121 cggatcttac gctgtcatcc ttttgtcaaa ggcggtatcg attatgtacc attgacattc
    18181 cgattaacgc gaaataagaa agataaaaaa gaaccagatt ataaaaaggt tgtgaaaaat
    18241 acgggtagca ctgagcgata aagaaaaaga ggaaatttat tgtcaaagta acaactccgc
    18301 aactacttat atgggggaca aaaaatgatc gaagaactaa acaagaaaga tttaccatgg
    18361 tccttgttat tagatgcaga tcctgataaa gaaaaagttc aagtatatgt ttccagaggg
    18421 agtggctgga tatggaaaga aaaagaaaaa acaataggtg tactgatcta tgtggcaagg
    18481 gaaaaagaat acgaaatcgt caatgtagca gtaaccccaa cgtatcaagg aaaggggatt
    18541 ggcggaaaat tacttgagac agcttttcag aagctttcac agctagcttc aaaccaaact
    18601 cggatcatta tacgtactgg cactactagt agcgctgcgt tacacctcta tcaaaaaatg
    18661 ggctatgtgg agattggtcg agtcaaagac tattttatcc atcattaatc tgagcccatt
    18721 tttgaaaatg gagatcaatt aagggatcaa gtgattttag caattcactt acctaaaaat
    18781 agctttaact aataaacttt caattcgaga aaatgctttc aaatgctaaa tagatacatt
    18841 acaatagctt tagcaaacaa aaaggagaat atttttatgg gaatattagt ttttgatttt
    18901 ggtggatcgg ctgtcaaata tggttgttgg gatggcaaag agattaaagg taagggacaa
    18961 tttgcaacgc ctgaatcttg ggaagaaatg aaaacgcaat tatttcaagt ttataaaaaa
    19021 atactcgttt cttttgaagt cgaaggaatt ggaatcagct ctcctggagt agtgaataat
    19081 gagaaacagg tgattgaggg aatcagtgcg attccttata ttcatggatt taatattttc
    19141 cgagatttgg aaactctgtt tcaactccca gtaacgattg aaaatgacgc caactgtgcc
    19201 ggaatggctg aattttatga aggtgctgcc cgtgattatc aagatgttgc ttttgttgtg
    19261 gcaggaactg ggattggcgg ggcgttgttc acgaaaggac aaataaataa aggggcacat
    19321 ctttacggtg gagaatttgg gttgatgttc ttagatggcg ataagacatt cagtaaactt
    19381 ggtacagcgg tacagatggc ttggcgttat tgtgaacgca aaggtctcga taaaaatgct
    19441 tataccggaa aagatgtctt tgaattagca gaaactggag atgcaatagc aaaagaagaa
    19501 gttgagagtt tttatacgta tcttacaaag ggattattca gtattcaatt ttcgtttgat
    19561 ccagaagtga tcgtattagg cggcggggtt tctgcaaaag aagggctcat tgatgagatc
    19621 aaaagccgaa tgaagaagct gacagaacaa ttcgatctgc atgatttcga accgcagatt
    19681 ttattatgtg agtacagaaa cgatgctaat cttgtaggtg cagcagcaaa ctatctcgct
    19741 gtacgtcaag gagagaatta agagctttag agaaggtgca gcttgcataa aagaagagaa
    19801 tgaaatgaat aggagatttt tacctaagga atgttttatt tgctgcagta tgataaaata
    19861 tctgagaggt gaaaatgatg tcaaaaaaaa ataaagatat tgaagtgcgt atagaagaag
    19921 tgaaaaaaga tatcaaaggt aagaattatg aagtcactca gttatttata gggaaaaaaa
    19981 tgattggtga gatcttagcc tatgggccaa aagaatatga aattttcttt ggtgaagaag
    20041 attttggaaa agaaaaaagt cttgaaaatg cgatagaaac agtgattcga cactggaatc
    20101 tgcacgaata aaaaaatatc aaaaaaactt tcaataaagg gttgcaaaaa tctggaaaat
    20161 taggtattat aactaagtca ggttgataca aacctttcgg aggagtagcg aagtggctaa
    20221 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
    20281 ttcattgggg tatagccaag cggtaaggca agggactttg actccctcat gcgttggttc
    20341 gaatccagct accccagtat
//
LOCUS       DAZKOW010000052        20290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000052 DAZKOW010000000
VERSION     DAZKOW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.052"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(107..1666)
                     /locus_tag="ACG912_001733"
     CDS             complement(107..1666)
                     /locus_tag="ACG912_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFQ0513049.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            1821..4055
                     /gene="pcrA"
                     /locus_tag="ACG912_001734"
     CDS             1821..4055
                     /gene="pcrA"
                     /locus_tag="ACG912_001734"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFQ0513050.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            4072..6108
                     /gene="ligA"
                     /locus_tag="ACG912_001735"
     CDS             4072..6108
                     /gene="ligA"
                     /locus_tag="ACG912_001735"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFQ0513051.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            6214..6519
                     /gene="gatC"
                     /locus_tag="ACG912_001736"
     CDS             6214..6519
                     /gene="gatC"
                     /locus_tag="ACG912_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFQ0513052.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            6519..7991
                     /gene="gatA"
                     /locus_tag="ACG912_001737"
     CDS             6519..7991
                     /gene="gatA"
                     /locus_tag="ACG912_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFQ0513053.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            7988..9418
                     /gene="gatB"
                     /locus_tag="ACG912_001738"
     CDS             7988..9418
                     /gene="gatB"
                     /locus_tag="ACG912_001738"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFQ0513054.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            9428..10483
                     /locus_tag="ACG912_001739"
     CDS             9428..10483
                     /locus_tag="ACG912_001739"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0513055.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            10594..11967
                     /gene="rlmD"
                     /locus_tag="ACG912_001740"
     CDS             10594..11967
                     /gene="rlmD"
                     /locus_tag="ACG912_001740"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFQ0513056.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            11970..12098
                     /locus_tag="ACG912_001741"
     CDS             11970..12098
                     /locus_tag="ACG912_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFQ0513057.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            12538..12726
                     /locus_tag="ACG912_001742"
     CDS             12538..12726
                     /locus_tag="ACG912_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFQ0513058.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            12804..13817
                     /locus_tag="ACG912_001743"
     CDS             12804..13817
                     /locus_tag="ACG912_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0513059.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(13884..14918)
                     /locus_tag="ACG912_001744"
     CDS             complement(13884..14918)
                     /locus_tag="ACG912_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0513060.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            15033..17258
                     /locus_tag="ACG912_001745"
     CDS             15033..17258
                     /locus_tag="ACG912_001745"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0513061.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            17276..19159
                     /locus_tag="ACG912_001746"
     CDS             17276..19159
                     /locus_tag="ACG912_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFQ0513062.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            19140..20114
                     /locus_tag="ACG912_001747"
     CDS             19140..20114
                     /locus_tag="ACG912_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFQ0513063.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         6839 a         3720 c         4350 g         5381 t
ORIGIN      
        1 aaaaaagaag aggctgggtt ttcccagcct cttctttttt tatagaatca tatagaatca
       61 agtagtgtag atgaatagtt gttgtaaaat agtttttcca tgttcgttaa aatgattgct
      121 tcaaatttgt gatagaagat ttagcctcga tttcctgctt gattcttggc aagtttgctt
      181 catcagtcaa gataaccaaa atatctccta cattcatgat cgtatcaccg tgagtcaaga
      241 tttcctgatt tcctctgcgt atagagatta gcagcatatt tttaggccaa ttgaagtctc
      301 gtaccattaa tccgtctaaa gaactctctg ccgttactgg aaaatcaaaa gttgtccttg
      361 caccttttag tgaggcggtc tgagtatttc caagtagacg ttccaataat gattcgtaaa
      421 ttggattacc gccgagaagg tcattgacga tataagcaac aagagcagcc aatcctaaag
      481 gcatcagatg attaagagag ccaaccattt ctgtcactaa aataattgct gtcagcggtg
      541 cttttccaat cgctgtgaaa taaccagcca tggcaataac gacaaaatcc ttgatgtaga
      601 ttggatccat cccgaaatca accataatgt ttccataaaa gctaccgatt aaagccccca
      661 atgtcaatat cggaaggaaa atcccccctg gaagaccccc accgtaagaa atcatggaaa
      721 aaacgaatcg aagaacaaac aaaccgataa tcataatgat tggcaaggaa gcatgtccaa
      781 aagctaaaac gatttgattg cctccgccta gagtactagg ccataaataa ccaattggca
      841 aaatcagcaa gaaaggaacg aacccgtgaa aataagctgg taaaggcaat cggttgtaaa
      901 attttggcaa agccagcaag acattctgat aaataaaacc taagatgcct agaagtactc
      961 ctaagatcac taaggaacca taatatttca acggcaaaga aggaaggtca cctagatata
     1021 aaacaggtct caagccgaaa aagtaaagag agacgaagtt agcagtgatt gctgaagcaa
     1081 atgatgtcag ccaaacaagt ggagaaaagg agtgatggat ttcttctaaa acaaagagta
     1141 atcctgcgat aggagcattg aatgctgctg caagtccgct acttgcaccg ctagaaatta
     1201 aaatcttttc ttctgaagat ggactccgaa acaattgtcc agctccttgt ccaatggatg
     1261 cgccaagctg tatagaaggt ccttctctac ctagaaaaag gcccgcacca actgaaagta
     1321 caccgccaat gaactttttc cataaaacag agaaccagtt cattgataat tccccgcgaa
     1381 gctgtcccac tacttgtgga atcccgcttc ccttgatatc cggttcattt ttgaccagat
     1441 aacccacgaa aaatgctgca aggatgcagc aaatagctag gccaattaag taaatcggct
     1501 gttcccgact catttcatag atacttcgaa ctaaatcgct tagcttttcg atagataatc
     1561 gaaacaggct aacgacgatt cctgtcagca gtccaacaaa tattcctttt agaataaaat
     1621 atatctgagt cttatctaat ttcttgattt ttcctttttt ttccattcaa acgcctcgct
     1681 ttcttttaat ttagcgcgaa atcaacgaat tgacaaggaa aaatccataa aaagaaacaa
     1741 atgattttga actagctagt ttaatgaaag ccaagaaaat gctataatta acaagattga
     1801 taaaagaaag gatgttgtta atgtcatcca aagctgaatt attaaacggg atgaaccccc
     1861 gtcagaaaga agctgttctt cacactgacg gaccattact gctaatggca ggtgcaggaa
     1921 gtggaaagac gagagtgctg acacaccgca ttgcctattt gattgaggaa aaagaagtca
     1981 atccttggaa catcctagcg attactttta ccaataaggc tgcaaaagaa atgaaagaac
     2041 gtgtcaatgc gattctcgca tctggaggcg aagatgtctg ggtatcgact tttcactcga
     2101 tgtgtgtgcg tattttaaga agagatgttg attttatcgg gtataaccgg aactttacaa
     2161 tcatcgattc ttctgaacag ctgaccttga tgaaacggat actaaaagaa ctgaacattg
     2221 atccgaaaaa atatgatcct cgaagtatat taggaacaat ctctcaagca aaaaacagcc
     2281 tgcagacccc tcaggatttt gcaaaaatgc aaggcagtta ttacgaagag attgcggcaa
     2341 aatgttatgc tgcttaccaa aaagagcttc aatataatca atgtatggac tttgacgacc
     2401 tgatcatgaa tacgattcga ttatttgaag aacatcctga ttcactcacg tattatcaaa
     2461 acaaattcca ttatattcat gtagatgagt accaagatac aaatcacgca cagtatacgc
     2521 tagtcaattt attggcaggt agatttagga acctgtgcgt cgtgggagat gcagatcaaa
     2581 gtatttacgg ctggcgcggt gcagatatgc agaacatctt ggattttgaa aaagattatc
     2641 cagatgctgc agtaattttg ctagaacaaa actatcgctc cacgaaaaac atccttagcg
     2701 cagcaaacca agtcattgaa aacaatagca atcgaaaacc aaaaaacttg tggacagaaa
     2761 acaaagaagg aaacaagatc acttactacc gtgcagataa tgaacgagac gaaacccgtt
     2821 ttatcgtaga ccggatgcaa gaagagattc gaagcaatca tcggaattac ggcgactttg
     2881 ccatcttgta ccggacgaac gcacaatctc gtgtaatgga agaaactctg ttgaaagcaa
     2941 atattcctta taaaatggtt ggagggcaca agttctacga tcgtaaagaa atcaaagata
     3001 ttttagcgta tttgaatgtg ctggtaaatc ctcaagattc aatcagtttt gagcgtatcg
     3061 ttaattcgcc caaacgaggc atcggaccag gatctatcga aaaactacgc agttttgctt
     3121 ctcttcatga atggccactg cttgaagcag cgcaaaatgt ggatctggca aatatcggtg
     3181 gaaaagcagg acagcaatta ggtgcatttg gagagatgat ccaagaagtc actcagatga
     3241 tcccatactt gacagtgaca gaattgacga aagaggtttt ggatcgtagc ggatatctag
     3301 aagacttaaa aatccaaaat acattagaag cacaagcacg catcgaaaac ttggaggaat
     3361 ttttaacggt cacacaagaa ttcgacaaac aattcgaaca gcaaaatgaa gaagacgctg
     3421 atgcaccaga agaaaaacta acggtcttct taaatgatct ggcacttgtt tccgacattg
     3481 acaatttaga agaagatgct tcccaagtga cattgatgac tttgcatgca gccaaaggat
     3541 tagaatttcc agttgtattc ctgattggtc tagaagaggg ggtttttcct ttatctcgag
     3601 cattgatgga agaaagcgaa ctggaggaag aacgccgtct cgcatatgtg gggatcacgc
     3661 gagcagaaga agcgttgtat ttaacaaatg ctttctccag aacattatat ggacggacgc
     3721 aatataaccg accttctcgt tttgtagaag aaatcgatca ggagttactg gaaatcgaag
     3781 gcatgcgtcc tacgcctaaa aaaacgcctg tattcgctaa aaaaacagca tatagctata
     3841 aacagccaga gacagcggtt gttccctcta aaagtgctac tggaggggaa aataacagct
     3901 ggaaacctgg agataaagtc aaacacaaaa aatggggtct aggaacagtc gtccgagtaa
     3961 gcgggacgtc gaaggatcta gaactagatg tggctttccc aagtcaagga gtaaaacgtc
     4021 ttttggctgc ctttgcgcca attgaaaaag cttagtagtg gagggtaaat gatggaacat
     4081 gaaatgacga ttgacgaagc tgctaaacga gcagaagaac ttcgtacaag actgaaccaa
     4141 tggtctcgcg aatactacgt agaagataaa ccaacagtgg aagactatgt ttatgataaa
     4201 gaatacgcag aattagtagc aatcgaggaa caatatcctg atttgatcac gtctgattct
     4261 cccactcaac gagtaggcgg aaaagtatta gaaggttttg aaaaagtcac tcatgatatt
     4321 ccgctttaca gtttgaacga cgttttcagc aaagaagagc tgatagcttt tgatcaacgt
     4381 gtacaaaaag cagtgggacg tgtagttgac tattgctgtg aactaaaaat agatggactt
     4441 tccgtttctt tgcgttatga agatggaaat ttcgtgagag gtgcgactcg cggggatgga
     4501 actgtaggag aaaacatcac tgaaaaccta aaaactgtcc ggtcggtccc aatcaaatta
     4561 aaagaaccaa tgaacatcga agtaagaggc gaatgtttca tgccaaaacg gtcatttgtc
     4621 caattgaacc aagacagaga agcagaggga aaagatatct ttgcgaatcc tcggaacgcc
     4681 gcagctggtt ctttgcgtca gttagattct aaaatcacag cgaaaagaaa tctggacact
     4741 tttttataca cagtagctga tttcggtcct atgcaagcaa aaacccagta tgatgcgcta
     4801 gaagagctag aaaaaattgg attccacacg aatcgggaaa aacgtctatg tcattcgatt
     4861 gatgaagtgt ggtcatacat tgaagaatat catgacaaac gtgtggattt gccctatgaa
     4921 atcgatggaa tcgtcatcaa agtgaatgaa ttctcgctac aagatcaact tggttttact
     4981 atcaaagcac cgagatgggc agcagcttac aagtttccgc cagaagaagt tgaaacgcta
     5041 atcgaaaaca tcgaatggac agtcggtcga acaggggtag taacgccaac agctatcatg
     5101 acaccagtcc gagtagcagg aacgacagta agccgtgcca gcctgcataa cggtgattat
     5161 attaagttga aggatatccg cttgaaagat acagttctga tttataaagc gggagacatc
     5221 atcccagaag tttcgcaagt cgtgttagat aaacgaccta aagacagcga agaataccaa
     5281 ttgcctacac attgtccagt ttgtggaagt gaacttgtcc atttggatga agaagtagcc
     5341 ttacggtgca tcaatcctaa atgccctgct caaatgaaag aaggtttgaa ccactttgtt
     5401 tcaagaaatg cgatgaatat cgatgggttg gggccgcgcg tactagaaca gatgtatgat
     5461 aaaaaactag tagcagatgt tgctgatctt tacaaattga cagaagaaga gttattgacg
     5521 ttggacaaaa taaaagaaaa atcagcaaat aatattttga cagcaatcga caacagcaaa
     5581 gataattcgg tcgagcggtt gattttcggg ttaggaatcc gacatgtcgg agcgaaagca
     5641 gcaaaaattt tagcagaaca tttcggtgat ctagaaacat tgagtaaaag tgactatgag
     5701 tcgattattg cgcttgatac gatcggtgat atcattgccg acagtgtagt cacttatttc
     5761 agtaacgaag aagtccatga attgatgaat gaattaaaac aagcaggcgt caatttcgaa
     5821 tacaaagggc ttcgaaatgc ccaattgcaa gaagtcgagt ctccttttaa agaaaaaaca
     5881 gtagtcctta ctggtaaact aacacgattt actcgtgaag aagcaaaaga gacgatcgaa
     5941 aatcttggcg ggaaagtgac tggtagcgta tcgaaaaaaa cggatatcgt ggtagcagga
     6001 gaagatgctg gcagcaagct gaccaaagca caagaattag gaatcgaagt ttggacagaa
     6061 gatcaaatgg cagatgcatt ggctaaaagt cgctcagtag aagaatgaaa aatgagtttt
     6121 ttcagacaaa tttcaataaa caatttcaga cacggcactc ttgtgttaaa atagagagca
     6181 aagtttttaa ctcgtttaga aagaaggggt agtatggcga ttagtgaaga acaagtgaaa
     6241 cacgttgcca aattagcaaa acttgcgttc gcagatgaag aacttgcaag ttttaccgac
     6301 caattaggta aaatcatcga tatggttgaa atgctggaag aagtagatac ggaaggtgtt
     6361 ccatttacgt cgaatgtggt tgaaacagta aacgtgatgc gacaagatcg ggcgcaagct
     6421 ggatggaacc gcgatgaatt attgaaaaat gtgccggaaa cagaagacgg ctttatcaaa
     6481 gtacccgcaa tcatcgataa tggggaggct ggcgcataat gacagaatta cacaatcgtt
     6541 ctctagaaga actgcatcat atgcttgtat cgaaagaaat cactgtacaa gatttgacaa
     6601 aaacaacatt tgaacgaatc aaagaaacag agcctgaggt cgaggcattc attacattga
     6661 acgaagaaaa agcattggaa caggcaaaag ctttagatga aaaagggatt gatgaaagca
     6721 acgtccttgc tggtattcca attggtatca aggataatat cgtaacaaaa gatatcttga
     6781 caaccgctgc atctaagatc ctttacaatt ttgaaccaat ttatgatgca acggtcatgg
     6841 ataaaatcta tcaagcagat atgatccctg ttggtaagct aaacatggat gaatttgcta
     6901 tgggtggaag tacagaaact tcttatttca agaaaacaaa aaatgcttgg gacccaacaa
     6961 aagtacctgg tggctcttct ggtggttctg cagcagcagt tgcagctgga caaattccag
     7021 tttctttagg tagtgatact gggggaagta tccgccaacc agcagctttt aacggcatcg
     7081 tagggatgaa acctacttat ggccgtgtct cacgttttgg tttgattgcc tttgcttcat
     7141 ctcttgacca aatcggacca atgacccgta cggtgaaaga taatgcatta gcattgacag
     7201 ctatcagcgg ttatgacgaa aaagacggta cttcttcagg cgtttcagtt cctgattttg
     7261 cagagggact gacaggcgac atcaaaggaa tgaagattgc tttacctaaa gaatacttag
     7321 gcgaaggtgt ggcccctggc gtaaaagaag ctgtattgaa agcagcagaa acattccgtt
     7381 cattaggtgc tgttgtggaa gaagtcagct tgcctcattc aaaatacggt gtggctgttt
     7441 attatatcat tgcctcatca gaagcaagtt ccaatttgca acgattcgat ggtatccgct
     7501 acggttatcg ctctgaaaat gtgaaatcat tagatgatgt atatgtcaac tcacgttcag
     7561 aaggattcgg tgaagaagta aaacgccgta tcatgttagg tacattctcc ttaagcgcgg
     7621 gttattatga cgcttacttc aaaaaagcag gacaagtccg tacattgatc aaacgagatt
     7681 ttgaaaaagt ctttgcagat tatgacttga tcatcgggcc atcttcacca acagttgcat
     7741 tcggaatcgg tgagaacatt aacgatccaa tcacaatgta catgagtgac atcttaacga
     7801 tccctgtcaa cttggctgga cttccaggta tgtctgtacc ggcaggttta tcagaaggat
     7861 taccagttgg attgcagatc atcggtaatt attttgatga aaaaacgatg tatcaagcgg
     7921 catatgcgtt tgaacaagca acagaattcc ataaacaaca accagctatt ttaggaggga
     7981 aagacgaatg aattttgaaa cagtcattgg actagaagtc cacgtcgagt taaaaaccaa
     8041 ctcaaaaatt ttctctccag cgccagctca ttttggtgca gaaccaaaca gcaatacaaa
     8101 cgtgatcgac tggggttatc caggcgtgct gccagtaatg aataaagctg cgctggaatt
     8161 cgggatgaaa gcagctttgg cattaaactg tgaaatctct aaagatactc attttgaccg
     8221 gaaaaattac ttttacccag ataatccaaa agcatatcaa atttcccaat ttgatcaacc
     8281 aatcggtcat gacggatgga tcgaaatcga agtagaaggt aaaaagaaaa aaatccggat
     8341 cgaacgtgtc catttagaag aagatgctgg aaaaaacatg catggtattg gcggttattc
     8401 ttacgttgat ttgaaccgac aaggcacgcc tttgatcgaa atcgtttctg aagctgatat
     8461 gcgttcacca gaagaagcat acgcttatct agaagcattg cgatcaatta tccaatttac
     8521 agaagtcagc gatgtgaaaa tggaagaagg ctctatgcgc tgtgatgcca atatttctct
     8581 tcgcccttat ggacaagaag agttcggtac gaaagcagaa ttgaaaaact tgaactcttt
     8641 atctttcgtg aaaaaaggtt tagcttttga agaaaaacgt caagcaaaag ttcttctttc
     8701 aggaggagaa atccaacaag aaacacgccg ttttgacgaa acaacgaata aaacagtttt
     8761 gatgcgtgtc aaagaagggt ctagtgatta tcgttacttc ccagagcctg atatcccgaa
     8821 attcgtgatc gatgacgaat ggattcaaaa agtaaaagct agtttgcctg agatgccagc
     8881 ttctcgtcgc gaacgctata tccgtgagtt tggacttcca gagtatgacg caatggtatt
     8941 gacacttaca aaagaaatgt ctgatttttt tgaagctgtt ttggaaaatg gtgcagatgc
     9001 caagcaagca tctaactggt tgatgggtga agtttctgct tacttgaaca gtgagaaact
     9061 ggaattagct gagacaaaat tgacacctga gaacttagca ggaatgattc gtttgatcgc
     9121 cgatggaacg atcagctcaa aaatcgccaa aaaagtcttc cgtgaattga tcacaaatgg
     9181 cggagaagcc caagaagtag ttgaaaaaaa tggttgggtg caattatctg atccgaacaa
     9241 actactgcca atcattaatg aaattttgga taacaaccaa caatctgtcg atgatttcaa
     9301 aaatggaaaa gatcgtgcgg ttggcttcct agtcggtcaa atcatgaaag caactaaagg
     9361 acaagcaaat cctggtgtcg tgaataagct acttaaagat gaattagcga aacgatagag
     9421 gctgaaaatg aaaaaagcac gcgtaattta taaccccacc tcgggaaaag aattattgaa
     9481 aaaaaatttg gcggatatcc tacaggcatt agaagaagca ggatttgaag ccagcgccta
     9541 tgcaactaca ccagaaccag attcagccaa gaatgaagca tgtagagtcg cagaactagg
     9601 ttttgatcta gtagtggcag ctggtggcga tggcacaatc aacgaagtgg ttaatggaat
     9661 cgctccgctt gaacaccgtc ctaaaatggc catcatccca gctggtacaa cgaacgacta
     9721 tgctcgagca ttaaaaattc ctagagataa tattaaagca gcagcagaag tgataaaaaa
     9781 acaccaaacc gtaaaaatgg acatcggcct ttctgataac agttacttca tcaatatcgc
     9841 tgcaggtggc tatttgacag aattgactta tgaagttcca tcagaactaa agagtatttt
     9901 tggttatctt gcttatcttg cgaaaggagc agagatgctc cctcgagtaa agccaatcaa
     9961 aatgcgcttg aagtatgatg aaggggaata tatcggaaat gcttcgatgt ttttcctagg
    10021 gctgacgaac tctgttggag gattcgaaca gatcgcgcca gatgccaaat tggatgatgg
    10081 gaagttttct ttaatcattg tgaagacagc aaatatcttt gagatcttgc atattgcagc
    10141 actcatgctg aatggcggtc gacatgtcga tgatccaaga ataatctata ccaaaacaag
    10201 cagtttgttc gttgaaacag aagatgatat caatcgacca gtaatgatca acttagatgg
    10261 tgaatacggc ggggaagcac cgatgcattt ccaaaatctt catcaacaca ttgagttctt
    10321 tgccaatacc gatcagattc ctgatgaagc aatcacaggt acagatgaag aagaattaga
    10381 ggttgtcagc aaagaattcg taaaagaagt cgaacgttta acggatgaag atatcgacgg
    10441 tgacggaaaa atagcagacg aaacacctaa tcaaaagaat taatcaaaag ccggtgctga
    10501 acgtagtaag gtcagtgccg gttttagttg tgtaattagc tgatttatag tagagttcag
    10561 taggattgaa aaataggctt aaaggagcaa aatatgacag aacagcaaat agtaaagaaa
    10621 aacgaacgat atacagtcga aatcgtcgac ttgagttatg aaggaatggg ggtagcaaaa
    10681 atcgatggtt atccattatt tatcgaaaat gcactccctg gagaagaagt agaaatacta
    10741 gtagtaaaag caggcaataa gtttggatat ggaaaagtcg aaacatttat tacacaatca
    10801 cctgatcgtc agcctgtaga aaacaatgca ttacttcgca taggtatcgc acctttagcc
    10861 catttgaagt atgaacaaca gcttgtcttc aaacaaaaac aagtagagca tgtaatggcg
    10921 aagattgcta agatgccaga agttcctgta cttccaacgg tcggaatggc agaaccattc
    10981 ggctaccgta ataaagcaca gatccctgtt cgccgtatcg atggacagtt agccaccggt
    11041 ttttacaaaa agaatagcca tgatctagtc cctatcgaag atttttatat ccaagatcct
    11101 gtcatcgatc aagcaatcaa agtagtcaga gatatcttgc aacggttcca agtacgagga
    11161 tataatgaag aaaaaaatga aggacagctt cgtcacatca ttatccgtcg tggtcattat
    11221 tcccatgaaa tgatggtcgt tttagttact cgtaaagaaa aattctttaa atccaaggaa
    11281 attgctgaag ctattcatga agaattgcca gaagtggttt cggtcatcca aaatatcaat
    11341 gaagaaaaaa caaatgtgat tttaggagac aaagaaaaag ttttatatgg cagagactat
    11401 atcgaagatc agctattagg aaaaacctat cgaatctcag caaagtcatt ttatcaagta
    11461 aatactcaac aagcagagta tctgtaccaa acagccatcg agtttgccga gttagataaa
    11521 aaagacattg tgatcgacgc ttattcagga ataggtacga ttggcttgtc tttagcagac
    11581 agagtggata aggtgtatgg gatggaagtt attccagaag caattgaaga tgcacagttc
    11641 aatgctttaa ataataaaat cgaaaatgtt cattatgaag tcggaaaagc agaaaaagtt
    11701 atgaaaaaat ggcaagaaaa agggatcaaa ccatctgtga tcgtagttga ccctccacga
    11761 aaaggattag atcatcattt tattgaatca gcaatcgaaa tgtcaccaga acgaatcgtc
    11821 tatatctcat gtaatccggc aacttttgca agagatgcta agttatttgc agaagcaggt
    11881 tatgccgtga agaaagttca gcctgtagat cttttcccgc aaacccatca tatcgaatta
    11941 gtcgcttcat tcgttaaagg agaataaaaa tggaccaaaa agaaaaaatg aaagaattga
    12001 tcaaaaagaa acaaggtgga ggccgatcca aacaaatgga aacacctaag aatgatcgta
    12061 aaaacatgag aaaaggccct aaaatcttta ataaatagac gtgctaaaga aagactaaga
    12121 agtgttaact tttgttattg tttcttagtc tttttagtta gtctacatta ttttggcgat
    12181 ttgccaataa ggactgttga gttgtgcaaa atcaaatctg ggtcagaatg aacgtcattc
    12241 tgtctcagat ttttcgttat ttcaatttga ttaactgatt gatcaaaaag atttttattt
    12301 tcatattctc aaatgattca acactaaata tacatgtttg acgcggaaat tattattcga
    12361 ttttggcggc atcagggggg atgacgacgt agcagaccca ttattttact caagaaaatg
    12421 tccgtgccta ttatttagaa tacgatagtg aacgtgctgg gagttttgtt cttttagaga
    12481 aagtctctga agataaatta gttgtcttag attttgactg taacgaaaaa tggcgaaatg
    12541 gaagagcacc agcaagtgat tgaccggatc tacgaagcga cacgatatct gcctttggat
    12601 cgtttgtgct taagtcctca atgtggattt gcttcaacag aagaagaaaa tgttttgttt
    12661 gaagaacaat ggaaaaaatt tcttttgtca aaagcattgc agaagaagtt tggggaaaat
    12721 cattaaatca tctaggagag cttgatagga aataacacta tggtataatc agaatgctta
    12781 atactaacta ctgagggagc cttatgaaaa agaaaattct taaatttatc ggatggttct
    12841 tcggaatcat tcttgggatc gcgtttgcaa tctttcttgc cttccagctt tcccccagac
    12901 ctggagcatt cttgatcaac catatgtttt caaatactgt tcaaatcact gataaaaaaa
    12961 cgttcgatca agcagaaaaa aacgtggcga aaaaaacaga tttgacgtat gagtccaagt
    13021 ataaagagaa tacttatgac atctactatc caaaaaacag caaggggcct gttcctgttc
    13081 tattttgggt acatggtgga ggattcgtcg gcggagataa atcgggtgtc aaagaatttg
    13141 cgacaaaatt agtttcagac gccaacatcg cagtggttgc gatgaattat gaattagcgc
    13201 ctgattcaga ataccctaat caagttcttc aagtgaatga actgatcaaa gatttattag
    13261 ctgaagaaaa agacgacaaa ttgctagaca tggaacaatt attttttggt ggagatagtg
    13321 caggttcaca aatcgcattg caatatgcag ctatacaaac gaatgatgcg tatgctaaac
    13381 aaatgaaaat caaacagcta cttccaaaag atagtctgaa aggaaccatt tcttattgcg
    13441 gacctgttga tttgaagcta acagcacagc agcattcgga tgatcgattt atgaagtttt
    13501 tcgtcaaaac cgttgcgtgg tcgttgattg gaacaaaaga ttggaaaacg agtgagcaat
    13561 tgaaagaagc tagtgtggct gaccatgttt ctaaggaatt tccgccaacg tatataactg
    13621 acggaaatgc atactcattc caggagcaag gtcaagcatt agaaaatcgg ttgacagaat
    13681 taaaggtacc cgtgcaatcc ttgttttata cagatacaaa aaaagagatt acccatgaat
    13741 accaatttga ttacacgcta aaagaatcgc aaaattgtta ccagcagaca ttggattttg
    13801 tgaataagta taagtaagag gttgtgaaaa agccgtttcg ttttgagtat gaagaaacga
    13861 agcggctttt cacaacctct ctttcaatac ctttttgttc ttgggataaa actttcccta
    13921 acaatcaatc gtgcaggata agtcaaaatg attggttcgt cccgtatacc gtcaattcgc
    13981 tccaatgcca ttcgaacagc tgcttgccca attgatttcg cattgatttg aacactggtc
    14041 aagccagggg agaggtttgc gattgtctcg atattatcaa agcttgctac aacaatctct
    14101 tctccaattt ggatattttt ttgtttcaat gcccgataga caccaattgc cattggatcg
    14161 ctcccgacaa gaatggcagt aggaagaggg gattcatttt ctaatagatt agtcgcaagt
    14221 acctcacctt gtttttctgt ccagccacct agatgataag acatatattt ttctaagtga
    14281 tgatcgatca taaaatgctg ataggcacgc atcctctttt catttataga aatctgcttc
    14341 ccaccgaatt catccatatc gactttatat cccccgatat aggctattct tttatgacca
    14401 ttttctaaga acaattccag tacggtttta gtcatccgtt caagatctgc atacaccata
    14461 tcgacctctt gctggatgtc agggttatcc acgacgacta tttgcatgtt ttgttttaac
    14521 aaggcggtaa tcgattcttt cgtaacagtc ccaacaacga tcaccgcacc taactgatct
    14581 agatcttccc attgtttagg attatcagat agattataga ttcgattcat ccctaaatgc
    14641 agacgtcgag cttcttcttc catccctact cgcaaatgtt tgtaatagat atcctgttct
    14701 tcgctcgctt tgccgatcgt agagatcaca ccaatcgaag gcattctctt tttgatatat
    14761 ttacgagtac tgatgtcata atccaacgca gcagctgcct ctactatccg ttgccgagta
    14821 gtctgagcca catggatcgt ctgatcgtta ttcaacacac gggaaactgt agcaggagag
    14881 acatttgcct ttcgtgcgac atctctaatt ccaaccattt ttacacactc ctcaaaaaaa
    14941 taaaaaattt ttattgacaa aattgtttag taaacagtat acaggaatta taaaaaaaag
    15001 taaacggttt ccgttttgtt gggagtgaaa tgatggatat tacgcataga aaaacagata
    15061 aaaatttcat cttatttgat gaaaagagcc gagtcttcca tttacgaaat acatttcttt
    15121 cttatctgat aaagatagaa gaaagcaatg tattggcgca tatttatttt ggcaaaccag
    15181 tcaagcagta caaagacaat aaaaattatc cccgaagaga tcgtggattt tcaggaaatg
    15241 ttcccttaaa tcctgaccgg tctctgtcga aagatacact accgcaagaa tactcttctc
    15301 atggcagcat ggactttcga acgcccgcat cgatcattca gcgcaagaat ggatcagact
    15361 tactggatct gagatatgac agccattata ttacagatgg gaagccagag atcgaaggct
    15421 tgcctcaaac ttatgtgatg gacaagtcag aggcacaaac gttggtgatt tcacttaagg
    15481 atagagaaac agcgatttat ttcgatttgt tctataccat atttactgat agagcagtga
    15541 tcacacgatc tgtaaagata agaaatgaaa ccggagaaac aatcaaacta gaaaaagcgg
    15601 ctagtttcca gctagacttt gcccatacta ggcgatttga tgaagtcatt gctttgccag
    15661 gagcacatgt caatgaaaga cagatttcac ggcaatctgt tttatctgga acaaaagtat
    15721 ttgaaagccg tcgaggaacg agcagtcacc atatgaataa ctttattgct ttagttcatc
    15781 atcatacgac agaaaatact ggagaagcga tcggacttca atttgtttat tccggcaacc
    15841 attctttcga acttgaaaaa gatcagatca atcaattgag agtcgtcggt gggatcaaca
    15901 gccatcgttt ttcgtgggaa ttgaatgcag gccagtcttt tcaaacacct gaaatgatcc
    15961 tttcttactc aagtcaggga ttgaacaaaa tgagtcagat ccaccatgag ttgctacgtg
    16021 aaagaatcgc aagaggtcgt catcaatttg ctgaacggcc gattctggtc aataactggg
    16081 aagccactta ctttgacttc aatagtgaaa aaatcaaggc gatcattgat gaagcaaagg
    16141 agctaggcat cgaaatgttc gtattagatg atggctggtt tggaaaaaga gatgcggaca
    16201 attcttcatt aggagactgg ttcgagtatg aaggcaagtt gacaaatgga ttacgtgaaa
    16261 tagcagatta tgctcatagc aaaggattga aatttggttt atggtttgaa cctgaaatga
    16321 tctcggttga ttctgaactg tatcgaacac atcctgattt tcttatgcag gagccaggta
    16381 gaatgccttc tgcttccaga tcacaacatg tactggattt tacaaggtta gatgttcggc
    16441 agacgattga gaaacaaatg agaaagattt tagatacgat tcctcttgac tacatcaaat
    16501 gggatatgaa ccgttcgctt tcagatgtct attcgattac ccttgaccca cagagacaag
    16561 gagaagtagc gcatcgttat atgctcgggc tttatgaact acttgaacat ttgacaactg
    16621 attatccgga aatcttatgg gaaggctgtt ctggtggagg tggacggttt gatgccgggt
    16681 ttatttatta tatgccgcag tcatggacaa gtgataacac ggatgcagtg gagcgtatga
    16741 aaatccaata tggaacaagc cttgcttatc cgatttcgtc catcacagct catgtatcag
    16801 ctgtaccaaa tcatcagacc gacagatcta cttcgttgaa aacaagagga gaaacagcta
    16861 tgagcgctgt ttttggttat gaactggatt tgacaaagct gtctcctgaa gaagaaaagc
    16921 aagtaaaaga ccaaattata tcctatcaaa cgattcgtcc ggtgatccag tatggccatt
    16981 attatcgttt ggcgagtcct tttgaagaaa atattgccgc atggatgttt gtgtctccta
    17041 aacaagatga agctattgtg tttttaggga ggatactagc ttcagctcaa ccagcgttcc
    17101 atgaagtata tctgatgggg ttagatgatg aggcacttta tcaggaacag acctcgaagc
    17161 ggatattttc gggggccgaa ttgatgacag ttggacttta cttccccgat tttcaaggtg
    17221 atttccaaac agaactgctt catttcaaaa agttatgaga gagaaggaaa aaagtatgaa
    17281 agcaaatata aaatggttag atgatccaca agtttttaga gtgaaccaat caaatgcaca
    17341 tagcgatcat gtttgttatg caacaaggga agaagtagaa accacatcct cccttaaaca
    17401 gtctttaaat ggatcatggc gtttttgtta ttcgccaaat ccttcggtac gaccaaaaac
    17461 attttataaa aaagattttg atgcctgttc ttttgatgaa atcactgttc ctagtcatat
    17521 tgaattagca aattatgaca agatccatta catcaatacg atgtatccgt gggagggaca
    17581 tgtatttcgc cgtccccctt tcacgacaag ccctgagaaa gaggcggaag ggatcttttc
    17641 agctgctaaa gacaatccgg ttggttcgta tttgacgact tttcgattag aagaaggtct
    17701 aaagggaaaa caaatctcga ttcattttgc tggtgtagaa caagcaatgt ttatttggtt
    17761 gaatggtcat tttatcgggt atgccgaaga ttcgttcact ccgtctgaat ttgatttaac
    17821 accttatttg gaagaagaaa atattctagc tgtggaggtt tacaaaagaa gtacagctgc
    17881 attcttggaa gatcaggact ttttccgatt ttttgggatc ttcagagatg tcttcttagt
    17941 tgctcgagga caaaaccatt tagaagatct ttggatcaaa cctcgcttag atgacaaatg
    18001 tcaaaatggc aaacttgaag tagatttgtc ttttgagcaa gtagccaaaa acagtcagct
    18061 gaatgtagcg atactagatc ctgatgggaa ggaaatctat caacagcaat atccgttaaa
    18121 agagcaaatg acgattcgca tcgatggatt tgaaaatgtg gcactgtggg ggcattttca
    18181 gcccaattta tataagcttg aattggagac gatagacgaa gaaaaaagag taacggagta
    18241 tattgactat tccttcggtt ttcgtaaaat cgaaatcaaa gataaaatca tctacttcaa
    18301 tggaaaacgg atgattatca acggggtaaa tcgacatgag tggcatccga aaaaaggacg
    18361 cgccatcact atagaagata tggaaaaaga tttggctatc ataaaaaatg caaagatcaa
    18421 cggcgtacgg acatctcatt accccaacca gatcacttgg tattatttat gcgaccaagc
    18481 aggaatccat gtgatggcgg aaacgaacct cgaatcccat ggctcatggc aaaaaatggg
    18541 cgcatttgag ccttcttaca acgtaccagg tgcagtacct cagtggcagg cggctgttct
    18601 tgatcgagca aaaacgaatt tcgaaacatt aaaaaaccat ccaagtattc ttttttggtc
    18661 tcttggaaat gaatcttatg cggaagaaaa cattcgacaa atgaacgatt acttcaaaaa
    18721 agtagacccc gatcgacttg tccattatga agggaatttt ccaaatccag cattcgaaga
    18781 ttcgatttca gatgtaaaaa gctgcatgta tgcgccacca gaagagatca ttgactacct
    18841 tgaaaataaa gcggaaaaac cgttcatctt atgtgaatat atgcatgaca tggggaattc
    18901 gttaggtgga atggattctt acatgacgtt gcttgatcgt tatgaaatgt accaaggagg
    18961 ctatatatgg gattttaaag atcaggcgat ccaagtgact gatccaatca caggcagaga
    19021 agtgatgcgc tatggcggtg actttgacga taaaccaagt gactatgaat tctcagggaa
    19081 tgggatcgtt tttgcagatg gacaagaaaa acccgccatg caggaggtaa gatattatta
    19141 tgaaaaatac agtaaataaa agtcatatgg atacggaaaa agttgcaatc gtcttcggcg
    19201 actgtacatt aggtgtcaaa tcggggaata cgcattatat tttttcttat acaagaggcg
    19261 gactggaatc gctcaataaa aacggaaaag aatggctata ccgggaaaca cttccagttt
    19321 tttggcgagc aacgaccgat aatgacagag gtagcggatt tggatttaaa tccggtatgt
    19381 ggttaggagc cggattgtat ccaaaagtca aagaaataga agtactggtg gacaataaaa
    19441 agatcgattt gccgattgct ccagtaaata atcaatattc aagtaatgaa tacgctgaaa
    19501 cggttgaaat catttttaca ctggaaatca acacagtacc aaaaacccaa gtgacaattt
    19561 cctaccaaat cgctggagag ggtaaaatgc ttgtcaatat gcattattat ggacaaaaag
    19621 gattaccgga attacccgca ttgggtttaa ggttcattat gccaacgaaa gcaaaatttt
    19681 ttgagtatga aggcctatca ggggaaacat atccggatcg gatggctggt ggtgtcgaag
    19741 gtatttataa agtggcagga ctccccgttt ccccatacct tgttccccaa gaatgcggca
    19801 tgcatatgga aacgaaatgg gtagaactaa caagaaatca aacgttggat aatacggata
    19861 aagatcaaga agatttttcg ttgcgatttg aacagcagga tacgccattt gctttttccg
    19921 ctttaccatt caaagcagaa gaactggaac aggcaacaca tatagaagaa ctaccgccag
    19981 aaagacgaac ggttcttacg atttttgcaa aagtaagagg agtcgggggt atcaatagct
    20041 ggggagcgga tgttgagtca ccttaccgaa tcagcagcga agaggaccat gaaatcggct
    20101 ttattattca gtagcaatct ttagattatt atttgaaaaa cggagtttac aagacaaagc
    20161 tgctttattc tatatgtcat gaacaagaga gtaaaaaagt tctcagatac ttttgttcat
    20221 gactattttc ttcaataaaa ccaattgtta tttttctgaa aaacaaagtt aactatcaga
    20281 aaattaaaaa
//
LOCUS       DAZKOW010000053        19694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000053 DAZKOW010000000
VERSION     DAZKOW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.053"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACG912_001748"
     CDS             complement(<1..210)
                     /locus_tag="ACG912_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0513064.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="ACG912_001749"
     CDS             complement(216..446)
                     /locus_tag="ACG912_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513065.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="ACG912_001750"
     CDS             1063..1725
                     /locus_tag="ACG912_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513066.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="ACG912_001751"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="ACG912_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFQ0513067.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="ACG912_001752"
     CDS             5145..5459
                     /locus_tag="ACG912_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0513068.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="ACG912_001753"
     CDS             5472..5846
                     /locus_tag="ACG912_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFQ0513069.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="ACG912_001754"
     CDS             5856..6200
                     /locus_tag="ACG912_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFQ0513070.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="ACG912_001755"
     CDS             6271..7623
                     /locus_tag="ACG912_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFQ0513071.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="ACG912_001756"
     CDS             7683..8825
                     /locus_tag="ACG912_001756"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFQ0513072.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="ACG912_001757"
     CDS             8850..9104
                     /locus_tag="ACG912_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513073.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /gene="mobT"
                     /locus_tag="ACG912_001758"
     CDS             9360..10544
                     /gene="mobT"
                     /locus_tag="ACG912_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFQ0513074.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="ACG912_001759"
     CDS             10541..10678
                     /locus_tag="ACG912_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFQ0513075.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="ACG912_001760"
     CDS             10683..10904
                     /locus_tag="ACG912_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513076.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="ACG912_001761"
     CDS             10917..11417
                     /locus_tag="ACG912_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFQ0513077.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="ACG912_001762"
     CDS             11514..13361
                     /locus_tag="ACG912_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0513078.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="ACG912_001763"
     CDS             13364..14260
                     /locus_tag="ACG912_001763"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0513079.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="ACG912_001764"
     CDS             14310..14699
                     /locus_tag="ACG912_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0513080.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16185
                     /locus_tag="ACG912_001765"
                     /pseudo
     CDS             14686..16185
                     /locus_tag="ACG912_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG912_001766"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="ACG912_001766"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFQ0513081.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18872..19694
                     /locus_tag="ACG912_001767"
                     /pseudo
     CDS             18872..19694
                     /locus_tag="ACG912_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DAZKOW010000054        19007 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000054 DAZKOW010000000
VERSION     DAZKOW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.054"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            34..2187
                     /locus_tag="ACG912_001768"
     CDS             34..2187
                     /locus_tag="ACG912_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFQ0513082.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2156..3571
                     /locus_tag="ACG912_001769"
     CDS             2156..3571
                     /locus_tag="ACG912_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFQ0513083.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3604..4638
                     /gene="sppA"
                     /locus_tag="ACG912_001770"
     CDS             3604..4638
                     /gene="sppA"
                     /locus_tag="ACG912_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFQ0513084.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4640..5161
                     /locus_tag="ACG912_001771"
     CDS             4640..5161
                     /locus_tag="ACG912_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFQ0513085.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5158..5544
                     /locus_tag="ACG912_001772"
     CDS             5158..5544
                     /locus_tag="ACG912_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFQ0513086.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(6040..6112)
                     /locus_tag="ACG912_001773"
     tRNA            complement(6040..6112)
                     /locus_tag="ACG912_001773"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6077..6079),aa:Lys,seq:ctt)
     gene            6493..7062
                     /locus_tag="ACG912_001774"
     CDS             6493..7062
                     /locus_tag="ACG912_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFQ0513087.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7049..7909
                     /locus_tag="ACG912_001775"
     CDS             7049..7909
                     /locus_tag="ACG912_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0513088.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8099..8803
                     /gene="yycF"
                     /locus_tag="ACG912_001776"
     CDS             8099..8803
                     /gene="yycF"
                     /locus_tag="ACG912_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFQ0513089.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            8808..10643
                     /gene="walK"
                     /locus_tag="ACG912_001777"
     CDS             8808..10643
                     /gene="walK"
                     /locus_tag="ACG912_001777"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFQ0513090.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            10640..11956
                     /gene="yycH"
                     /locus_tag="ACG912_001778"
     CDS             10640..11956
                     /gene="yycH"
                     /locus_tag="ACG912_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFQ0513091.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            11957..12826
                     /locus_tag="ACG912_001779"
     CDS             11957..12826
                     /locus_tag="ACG912_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFQ0513092.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            12881..13690
                     /locus_tag="ACG912_001780"
     CDS             12881..13690
                     /locus_tag="ACG912_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0513093.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13793..14422)
                     /locus_tag="ACG912_001781"
     CDS             complement(13793..14422)
                     /locus_tag="ACG912_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0513094.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            14592..15884
                     /locus_tag="ACG912_001782"
     CDS             14592..15884
                     /locus_tag="ACG912_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0513095.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(15960..16622)
                     /locus_tag="ACG912_001783"
     CDS             complement(15960..16622)
                     /locus_tag="ACG912_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0513096.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            16847..17131
                     /gene="groES"
                     /locus_tag="ACG912_001784"
     CDS             16847..17131
                     /gene="groES"
                     /locus_tag="ACG912_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFQ0513097.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            17182..18807
                     /gene="groL"
                     /locus_tag="ACG912_001785"
     CDS             17182..18807
                     /gene="groL"
                     /locus_tag="ACG912_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFQ0513098.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6471 a         3063 c         3939 g         5534 t
ORIGIN      
        1 taaaaatttt ttagaaggag ttgtgttaga taagtgataa aatcatttcc ttttgtgcag
       61 caacaagatg aaaaagattg tggtgttgct tgcctttcga tgattttaaa atattttaaa
      121 actgatatcc cgattcataa attacgggac ctatctggga ctgatttaga gggtacttca
      181 gcatttggat tgaaaaagac atttgaaaaa ttgtcttttg attgtatggc tattcaagca
      241 gatagtgaga tctggaaaga aagagatctt atcttaccat taattgcaca tatattggtt
      301 gataacagct atatgcacta cgttgtaatt tataaaacga agggaaacgt actatggatt
      361 gcagatcccg ctaatggaaa aaatagaaaa acgattgaag cgtttcaaaa agaatggaca
      421 ggtattcttt tattacctat gcctagagaa acttatattc ctattaaaga aaaagtagct
      481 ggattaagtt cttttttgcc tataatttgg aaacaaaaag gattagtatt ccacatcgtt
      541 ttagcatcga ttttcattac gttttttggc ataggaagct catattattt tcaaggtatt
      601 cttgattttt tcatccctaa tcaagcacgt tcaactttaa atataatttc cattggcttg
      661 atcgctgtct atttatttcg ggtattgttt gaatatagtc gtagttatct tcttattatt
      721 ttaggacagc gaatgagtat ggcaattatg cttcagtatt tcaaacatgt gttaacttta
      781 ccaatgaatt tttttgctac gagaaaatca ggggaaatta tttctcgttt tcttgatgcc
      841 agtaagatta ttgatgcttt agcaagtgct acgttatcaa tatttttaga tattggtatg
      901 gtgttgcttg ttggggttac gttagctatt cagaatagta cgttgttttt aatcactcta
      961 tgctctttgc ctttttatgt agttgcaata ttcgctttcg tgaaaagcta tgaaaaatcg
     1021 aacagggaag aaatggcagc cggtgcagct gtaaattcta gtatcataga aagtcttaaa
     1081 ggaatagaaa caattaaagc atatagcggt gaagagaaag tatataaccg tatagaccat
     1141 gaatttgtta aattaatgaa aaaatcattt cgtacggtta cgcttgataa tattcaacaa
     1201 ggaattaaac atttgattca attaattagc agtgcattaa ttttatggct aggttcttat
     1261 tttgttatgg ataatagaat tagtctaggt caacttatca catacaatgc tttactagta
     1321 tttttgacag atcctttaca aaacattatt aatttacaag tgaaaatgca aacggctcaa
     1381 gttgcaaata atcgtttaaa tgaaattttc gcgattgatt cagaaaaatc gaaccagtct
     1441 tttgaatata tgctttctga acaattattt caacaagata tcactgtaaa aaacgtttca
     1501 ttctcgtata gcatgaaggc cccaacgttg aacgaaattt catgtacaat tccttcgcac
     1561 agtaaaacgg ctatagttgg agttagtggt tctggaaaat caactcttgc aaagttattt
     1621 gttcgttttt atgaagcatc agaaggatcg atccattatg gaaacattaa tcacttagac
     1681 attccttttc agatattacg cgatcgagtg acatatttgc ctcaagagtc atttttcttt
     1741 agtggaacaa tcattgagaa tcttatattc ggtttagata gcccaccaag tttcgaacaa
     1801 ataatagaag tttgtgagat agtccaacta aatgaattta ttaatcaaca gccattacgt
     1861 tacgagacat tgttggaaga aggtggaaat aatctttcag gagggcaacg acaacgatta
     1921 gccttagcac gggctttatt aaaaaatccg gacatattaa ttttagatga agcaactagt
     1981 ggattagata ccttgttaga acatgcaatt atgaattatc tcctcaattt aaaagaaaaa
     2041 acgattattt ttattgcgca tcatttacca atagcaaaag catgtgatca aattttagtt
     2101 ctccatgaag ggcatttggt agaaaaagga acgcacgatg aacttcgttt tgccgatggt
     2161 atctatcaaa aattatggga aatctaggag agggaaacaa tttatgcaga ataataattg
     2221 gttcgaacat tctagtatct atactaaaca acatcatagc ttctaccgtt gggtgttata
     2281 ccctgtaatt ctttttttat gtattattgg attgttttta acctttgcta aaaaagaggt
     2341 tgttattcgg acaacggctc aattgaaccc cgaaaaaata gagaagttac aagtgccatt
     2401 agaagctaaa ataatggaaa acttcctaaa agaaaatcaa tttgttcata aggacgatgt
     2461 gttggttaga ttggattgtt ctttaataga gaatgaaaag gctcagatag aacaagaaaa
     2521 ccaacgtatt actcaacaaa taaagatggc tcagctattt attgaaagta taagtaaagg
     2581 aaaaaatttg ttttcaacgg atgacagttt tggctacagt aatcaattaa agagcatgtt
     2641 gtcagaaaaa gaatcactcc gctacgcttt gaagcaaagt gaattaaatg atcaaaagca
     2701 attagaagta tacgaaaaga caaaaagaca actagaaaaa caaattgaga gttcagatag
     2761 taaattacaa gaatggcaac aagtacaggt agcttggagt aataatcaat cattaaaaga
     2821 tttttcaaaa gaaatgatgg caaactatga gaattggcaa gaacaactaa ataatgtttc
     2881 tgatgatcaa aaaaatcaag tgaaactgac aatttcagca agcataaatg aacaaattga
     2941 gcaactaaaa aaagaagtag aacagtatca gtcagaaaaa gctaaattag ttaaaccaac
     3001 tacttctgag aatgacagaa ttagtcaaac ggaaaaagga aagcaagagc tagaacaagc
     3061 aataacaact gtaaaacaaa cagttacaga gctaaatgaa aaacaagaga agaaccagtc
     3121 agttatcaaa agtttggacg atcaactttc aaaaggaata ctaaaagctc ctgtatctgg
     3181 aacggttcat ttaaatgaag aaacaaaggg acaagcagaa ctagccaaag gaacagtgct
     3241 tgcagaaatt tatccgatcc acgaaaaatc aaaaatgaaa tttacagcac ttttacctgc
     3301 gaatgaaagt acctatatta aagaagggat gaaggttcat tttaaattgg atcaaaaagg
     3361 tggggctccg attacgatcg acggaacttt agatgaaatt tcagaaaatt ctactgccac
     3421 tgagaaaggt gttttttatg cagtgaaagg aacgttaaaa acaactcaat cgtttccgtt
     3481 tcgttatgga ttaactggag agttgtcttt aattgtagga gaaaaaacat attggcaaca
     3541 aattgtcgat gtgtttctaa ataaaaaata attattttta ctaagaatat aggaggagtt
     3601 tatatgaata aaagaagatg gattgctgtg ggagtagcgt gcggattatt gttactatcc
     3661 attatcgtcg cgctaattcc aggaaaagat aaagaggaag catcaaatac aaccctaaca
     3721 ggaataaata agatttttta cggatctaac gaaatcacag aagaaacgtt agaagaaggc
     3781 gcatcaaata aaaagattgt caaactatcc gtcaatggag ttattgcaga tacaggtgaa
     3841 agcaatcttt ttagtaggga acaatataac catcagaatt ttttaacaca gctaaagaaa
     3901 attcaagaag acaaagcggt taagggagtg ttattagaag taaattcacc tgggggagga
     3961 atatatgaaa gtgctgagat tgctaaagaa atggcaaaaa ttaaaaagct agatattcct
     4021 atctatactg catttaaaaa tacagcggca agtggagggt actacatttc tgctggttca
     4081 gataaaatat ttgctacgga agaaacaaca actggatcaa ttggtgtcat tatttctggt
     4141 ttaaattatt ctggcttatt agaaaaactt ggcgtaactg atgcgacgta taaaagtggt
     4201 gcattaaaag atatgatgtc acctcaacac aaaccttcag aggaagagaa taaagttata
     4261 caggaatttg tgatgagtgc ttatgatcgt tttgtcaatg ttgtagcaaa aggaagaaat
     4321 atggatacta atgcagtgaa agaacttgcg gatggaagaa tttatgatgg taaccaagca
     4381 gtagaaaatg ggttggtgga tcaaattggt tactctgaag atgcattaga ttccctaaaa
     4441 aaagagaaga aattaactga tgcgacggtt atagaatata aaaatgacac aactggcttt
     4501 gcttcttctt ggttaggtaa taagattgct gaatggcaag ggctaaaggc aactacatca
     4561 gatcgtgtaa tttcagtttt tgaaaaactt ggattttctg atactccaaa accaatgtat
     4621 tactatggag ggctataaaa tgaataagaa gagtggcatt gaaactttgc ctaattatgt
     4681 atttgcggga ttttggttac gattttttgc ttttcttatt gatttaattt gtattagcat
     4741 catcacccat gctactatag gtctctttta caaaatcatg ggactggaat ctgacaaatc
     4801 cattattact ttttatggta tagcgtcatt agctatttat ttaacctatt ttgtactttt
     4861 gactaagctg aattcaggac aaacaattgg gaaaatgatt tttgggataa gagtagtgag
     4921 ctttgaggaa aaagagctaa gttggcaaac tatctttatt cgtgagtgta tatgtaggtt
     4981 tatattaaaa aattttgtat ttcaaattgg gtatttggtg gctgctttca cgcccaagaa
     5041 acaacatatt ggggattatt tttctaacac gtcagtggtt gttattcgtt cattagaagc
     5101 tattaactat ggtcaaaaaa tttctaattc taaaacactt aatctggaga gaattaaatg
     5161 aataagagaa aaacagagtt tattttgatt ttaattagtg gaataacagg gattttatgt
     5221 agtcttttag cattgatggg tggtatgttt ctatcttcta ttgattcaac tactttaaat
     5281 actacagaac taaccagtaa ggatatagat atgttagtca atctaagcgg gaatgttgtt
     5341 ttatttggag gaattaccct agttttttcg ataatgattt tcataatagg ttttttagta
     5401 agaaaaaatc aaaaaacagt ggtctatggt attagtttac tcattttatc tattattcca
     5461 ttttttcttt taacattatt gtgggtaatt cctggagttt taggtattat tgcgggattg
     5521 atgcttctaa taagaaaatc ttaattaggc aatgtgtaga caacgtgaaa cgattaatca
     5581 attaaaagct aaatacgttt tgagaaatga taacttgtag tcgatggaag acatgtagat
     5641 aaaaaaacta cgtgtctttt atttattcta attttttcga aaaaaagaca ctctctgatt
     5701 gttgtattaa tggaaaaggg gaaagcagca agtaaattta gtttttctat taaatattct
     5761 tcacttttta accttttata gtacttagat ttaatcctat atgtttagag agttcattgg
     5821 cagcatgatt ttgcaaagct tctcgaatta ttgttatatt agaaacttta ctgttcataa
     5881 ttatgcttct tttttaactc aaagtccaaa gggctacagt ggactttgag ttaaaaataa
     5941 aaagccctag ttattttgaa aaataaaaaa agaacagcgt aaatgttttc gaaatgcttt
     6001 cgcaccaaca tttacctgtt ccgtgggttt ggcacccaat gggccgtgag gggctcgaac
     6061 ccgcgacccg ctgattaaga gtcagctgct ctaccaactg agctaacggc ccgtacaaaa
     6121 agtacttaat aatagtagca ctaagaattt taaaatgcaa gactacattt aaagaaaatc
     6181 agaaaaatct ttacttcctt ataggaaatc attcatagat tattcacatt tgctcgctat
     6241 tatataagca taccaacaaa caaagttctt gcaagagaac aaaccctaac acttttttca
     6301 ttttttactc ctttcccgcc gtctccccgg cgggtctcct gttcttaacg ataaaagaca
     6361 agaaggctta gccttcttgt tttttgtttt catggaatcc cgctaatcaa atcacctagt
     6421 taaagcaact agattgaatg tttatcataa gtctgatatc atgtttatga aatgaaaaaa
     6481 ggagcggtta tgatggaaaa aatgcgtcag caattaatgg agtgggggaa ggaactagaa
     6541 acattccgtc tgcctcactg ggaagaactc cctgacttag aactttatat ggaccaagtc
     6601 agaacgttag ttgatcgtta tctttcacct gtgatacaag gagaaaagca tccattgctt
     6661 acctcttcta tggtaaacaa ttacgttaaa ctaggtttga taccagcacc agttaaaaaa
     6721 agatataaca aagaacacgt agcattttta cttgcaatca ctacgttgaa acaagtattg
     6781 actattcctg aaatcaaaga agggatattg ttccaaggaa aaacagtagg aattcgtgaa
     6841 gcctataatc tattttgcga tgaacaagaa gcagctgtgt ggatggtcag ccaattagca
     6901 caaggtaaat ctcatccgca aaaattcgac caggcaaccc ctgtagaata tattgctgta
     6961 agagcggcaa ctttatcgtt tgcaatgaaa cttcttgcgg aaaaaacgat tgttctagaa
     7021 accgaatatt taaaagagga gaaaacaaat gaaaaacaat aaaatcgcaa ttttagtaga
     7081 ttcaggtacg gatgttccag aagaattgat aaaaaaatac gagatgtata tgatcccatt
     7141 gaaaattatt tataaagatc aggtatatac agataaaatt gacattaccc ctgaagagat
     7201 ctatcaacgt ttgccagagg aaatccctgg tacatctttg ccggatggtg agtcgatcac
     7261 agaaatcttt gagcaaatca aaaaagacgg ttatgaaaag gtacttgcgg taacaatttc
     7321 cagtggattg agcgggacat ataatgtggt tcgtttaatt gcacaacagc aagaagaatt
     7381 ggaagcatat gtactagata cgaaaaatat tggtattggt gcaggttttt ctgcgatcca
     7441 agcagcaaaa tggctggaag aaggagtaga atgggatcaa ctgatcagta atctgaatga
     7501 gttagtcgaa agaacaaaag ttttctttaa tgttgcgaca ttggaatatc ttcaaaaagg
     7561 tggacggatc ggtttagttg cttctattgt aggaaccgct ttaaagctta acccaatcat
     7621 ctcatgtaat gaagaaggga tttattacac agtaggaaaa gctaggggac gtaaaaaaag
     7681 tttggataaa acggttgctt acgtcaaaga aaaaatcggt aatgcaaagg tcttcaactt
     7741 agctgttgcc catggggatg cgaaagaaga agcaattgaa atggaagcga gacttaaaag
     7801 agagttccca caagcaaacc aaatctactt tggccagatt tctccggcat tagtagtcca
     7861 cactggtccg ggattattgg gtgtcggtgt acaagtttta gaaggataaa tcaaaaaaag
     7921 gaaacactga ggtgaaaatc agtgttttcc ttttttattg aagttagaac aaaaaataat
     7981 aaagtatttt gcactttcat tttaattttc tcttttgttt tataggcaaa gatgaaacaa
     8041 aagcagaaat acggtataat gagaaggtat gacaataata tataagaggt ggctctttat
     8101 gaaaaaagtt ttagttgtcg atgatgagaa gccgatttcg gatatcgtca aattcaattt
     8161 agccaaagaa ggctatgacg tttatactgc atatgatggg gaagaagcct tggaaaaagt
     8221 tgctgaggta gaacctgatt tgattttact tgatttgatg ctacctaaaa tggacggctt
     8281 agaagttgct cgtgaggtac gcaaaactta tgatatgccg atcattatgg taacagccaa
     8341 agattctgaa attgataaag tcctaggctt agagttaggc gccgatgatt acgtgaccaa
     8401 gccattttcc aaccgagaat tagttgcacg tgtcaaagca aatcttcgtc gcggtgccac
     8461 ggctgcgaag gaaccagaag aggcagcacc tgcggaattg acgattggtg atttgacgat
     8521 ccatccagaa gcttacatgg tgacgaaacg aggagaaaca atcgagttaa cacatcgtga
     8581 attcgaacta ctcttttact tggcgaagca ccttggacaa gtgatgacaa gagaacattt
     8641 attacaaacg gtttggggtt atgattattt tggagatgta cgaacagtgg atgtaactgt
     8701 acgtcgtttg cgtgaaaaga tcgaagataa tccaagccat ccaaattatt tagtgacacg
     8761 tcgtggtgtc ggttactatt tgcgtaaccc agaacaggag taattggatg aaaagaaaag
     8821 tccgcttttt tcgatcagtc aattttaaga tcgctattac ctttattctg attttattga
     8881 tctcgatcga aattattggt gcctacttta tacgtggttt agaacgttcc acgatcaata
     8941 catttattaa agatatgaat caaacggtgg aatcattagc aacaacaatc agcccggaat
     9001 tgaatcgaaa agataatgca gatgatgaag aggtaaatgc gaatatcaaa agatttattg
     9061 aaaacagtgc gactagcgat atcatcgaaa tacgggtcgt tgacgaaaaa gggatcatcc
     9121 gaggaacaac cgatgtcaat gaacaaagcg ctgtgggaaa gaaaaatgat tatgttgata
     9181 tcaatgattt cacgactaaa cgttatgttg ctttagataa cgacgataaa cgggtgaata
     9241 tcaatgttca accgatcctg tccccgaccg gcgatgccgt tatcggggct ctttacgtca
     9301 aaagtaatat cgaacaaaaa tattcggaaa tcaataacac agcagtcatt ttctttactg
     9361 cctcactaat tgcggcattt atttcaatga tcgtatctgt tttagttgct cgttcgatta
     9421 cacagccgat cggtgaaatg agagaacaag ccattcgaat cgctagagga gactacagtc
     9481 ggaaagtcaa agtgcatgga caagatgaac ttggacagct ggctgatacc ttcaatcagt
     9541 tagctgaacg tattgaagaa gctcaagata caatggaagc tgaacgcaat cgtttggata
     9601 gcgtgctatc ccatatgaca gacggcgtca ttgcgacaga tcgcaggggt aaagtcatta
     9661 cgatcaatga tatggctgtt tccttgctag atatcaaaaa cgaggaagca atcggacaat
     9721 cgattttgac acttttagat atcgaagaag aatatacgtt gcggaaatta ttagaagatc
     9781 caaacgaaat ggtacttgac cgttcaacca gtacattgga aagtgatcag atgatcttgc
     9841 gagtagactt ttctatgatc cgtcgcgaat caggttttat cagcggatta gtagctgtct
     9901 tgcatgatgt gacagaacaa gaaaaaacag aacgggaacg tcgtgaattc gtctctaatg
     9961 tttcccatga acttcgtaca ccgttgacaa gtatgcgtag ttatatcgaa gcattgagcg
    10021 aaggtgcgtg gaaagacgag gaagtagcac caaatttttt gaaggtaacg ctagatgaaa
    10081 cagaccgaat gattcgaatg atcaatgacc tcttaaatct atctcgcatg gatacaggta
    10141 atacccagct tcaattagaa tatgtcaatt tcaacgagtt agtgaatttt gttcttgacc
    10201 ggtttgatat gatggtggga aatcaggaaa aaaattataa gatccgtcgt gaatttacgc
    10261 aaagagatct ttgggtcgaa gtagatacag ataaaatcat ccaagtagtc gataatatca
    10321 tgaataacgc gatcaagtat tcaccagatg ggggaacgat tacttgtcgc ttattagaaa
    10381 cgcataataa cgtgatttta agtatcaccg atcaaggatt ggggattcct aaaaaagatc
    10441 tgaaccgagt gttcgagcgg ttctatcgag tagataaagc acgtgcaaga gcacaaggtg
    10501 ggacaggact tggattagct atttctagag aagtgattaa ggcgcatcga ggcgctattt
    10561 gggcagagag caaggaagga aaagggtcga ctttttatat ctcgctgcca tatgaacctt
    10621 atgaggagga atggtgggaa tgagaatctc tgaaaaagtc gttcgtatag gactcatttt
    10681 gatggttgcg ttaagcatat acttctctta tgcaatctgg ctgagcccag caggaaaaag
    10741 ttcaatcaac gtagatgaga caaacactca aatgatcgag tctcaaagca atcgaaaaac
    10801 ttccgaagtc tttcttccgc ttcatgttac atggcaccat tcgggaatga tccaagaaac
    10861 caacagtgaa aatctcgtgt accgattgca attgatcatc gataaagcaa gatttggaaa
    10921 gctttctgaa gtagtttcag gagacaaatc acagtttgaa caggtgaaaa atctgaactc
    10981 tggttttgag ttggcctata atgcgccttt tttgctaaga gaatacaagg aagcatttga
    11041 tttagattta gatgtgtcga acttggcaaa agaaagcgaa aatatgtatt ttactagaat
    11101 acaattcgat caaggaaata aaaaaatccg atttttgaat tattcgaacc atctcgtata
    11161 tgaagcaaat atttcgttga attggacaga tatcaataaa gaattaacag caaaagacac
    11221 aaaatggaca gaaatgacaa cagaaccatc gacgatcgac acgcaatatg ctacgaaaca
    11281 ttcgatcaaa ctgaagaagt atagttatat cctgtcacag caaccctata cactttttag
    11341 aaatgctttt ttccagaaac cagatagcgt gaaaaataat gatgaaagca atgaattgat
    11401 tttttacggt gggaatgaaa caatgtctat ccattcggaa acgcaagtag ttgattttcg
    11461 tggagagctg ccagagaaaa aagaaaatgc tagtgtattt tctgaaagtt tcccctatat
    11521 tagcaaatta ggcagttctt tagggaacct cagatatttt gaccgcagtg acaatacgat
    11581 cgattatcgg atattcgtgg aaggcttccc ggtttttggg acagacagca gaggacagat
    11641 tggtgtggaa gttggcggtg aaacgaatgg ttctgtgcaa gtgaatatcc aaaccagttt
    11701 gaatacgatt caagtgccga taccttcaga cgaagaagta gaacttgcaa gtacgaatga
    11761 aatcatccag cagttaaaag ctaagggagc agatagcaac aagattggtt cagtgatcgt
    11821 tggctataca tggcaaaatg tcaaagaaac gaatcgtgtc gttgatctct tacctgaatg
    11881 gtatgtgaaa tataaagata actggtattc agctaacgac cttctggaaa aattacctgg
    11941 attggaggcg aattaattgg atttcaaaaa aatcgaatgg atatttttcg tcgctttttt
    12001 agggttgaat gtgtttttat tcagcagtta tcatgacgcg gtataccaag aaaacaatgt
    12061 cattcgctct aatcagactg aatcaatcca acaacgtctg gctagcgacg atatcactta
    12121 cacggggaag atttcgtctg agaacaagca aggctattat cttagcgcgg agcagaccaa
    12181 tcttagcact tcattagcga attatcgcaa ggctggtaaa agcggcttgc tggcaagtgg
    12241 tgtgttttta gacggtgatg ttttgacgaa atctttatcc agttcagatg ctgaaaagaa
    12301 cgtgatcgat ccagataaaa ttagtagttc cttggaagat tttttatcta acaaagacaa
    12361 tattttattc ggtaatgatt atgtgtacac gaaaaacttt tctcatttag aagaagaacc
    12421 actagaaatc caagcaagcc aagagtatga aggcatccca ttcaatgatg atacagcaaa
    12481 aatcgttctt tctttagaaa aaaacgataa tgattatagc atcacgaaat acatgcagac
    12541 gcatttatct gatatcgagc agctgcgcga aaagacggaa ctgtatacgg aagaggaagc
    12601 aatcaataca ttgtacatca ataacaaaat accaagagga tcaaaaattt tatggcgtca
    12661 gctagcttat tcctgtattc tgaaagtccg ggaaaaaaat gtgtatgttc ctgtttggca
    12721 tgtagcgatc gaaacaagcg ataaagtggt ccaaattgag agcgtcaacg ctttcagcaa
    12781 tacgatcgtg acaaacaata ctgtaccaaa agtggaagat caataaaaaa gcgagtataa
    12841 tgaaaaaagt ttacaaagta agaagggaag taggagtcaa atggctgaag aaaaaccagc
    12901 ttttaaaatc agtatcctag caagtggaag ttcgggtaat tcactttata tcgaaagtga
    12961 aaaaaaacgg cttttggtcg atgcaggact tagcggtaaa aaaatcactt ccttgatggc
    13021 tcaaattgat cggacaccgg accaattaga tggaatactt gtgacgcatg aacatcgaga
    13081 tcatatacat ggcgtgggtg tcttggctcg taagtatcat ttggatgttt ttgccaatca
    13141 gcagacttgg gaagcgatgg aaccactgat tggaaatgta ccagtggaac aaaaacatct
    13201 atttgagatg gggaaagtca gaacattcgg cgatatcgat attgagagtt tcggcgtctc
    13261 ccatgacgca gcggctccgc aattttatcg tttccataaa gaaaatcggt cattcgtcat
    13321 gttgacggac accggatatt gcagtgatta tatgcgaggt gtcattgaaa atgccgatgg
    13381 ttatttgatg gaaagtaatc atgatcttga gatgttacgc atgggacctt atccgtggaa
    13441 tttaaaacag cgtattttag gagatagagg acatctttcc aacgaagacg gtgcacttgt
    13501 catgactgat gtgatcggta atcggaccaa acgaatctat cttggccatc ttagcaagga
    13561 aaacaatatg aaagaacttg ctcatttaac aatggaaaat gttttgaaag aaaaagattt
    13621 tggcgttgga catgattttg aaatctatga tacagatcca gatgaagcaa ctgagctatt
    13681 ttcgatttaa acagttcaat caataaaaat ataaaaagca gaagataaaa tcaatcaggg
    13741 ttggttttat cttctatttt tattttttta gcatattaaa aaaataaccg tattatcgac
    13801 tgactacact gatgtgctgc ttttcatgtt tctcatccag tatctcatct actaatgcaa
    13861 tcgcgtaatc tgccatgctg atcgtactgt cgctagcagc gtttctttgt aaataatcat
    13921 cacttaacaa ataagtacct gtacgtttgc catgtggatc aaataaagaa gctggactga
    13981 tataagtcca atgtagattt ttagattgtt tcaattttaa caatccttcg tacatattga
    14041 aagctgttgg atagaagggt gcatttttag gtacttggtc aatcatacga gtcgtctttt
    14101 ctgtatctaa gaataacgaa gaggcacccc caacgacaat taagcgtgta tcggttcctt
    14161 caagcatctc aatcaaatgg gacaaagaag tttgatgcat ttcttctttt cctttgggag
    14221 cgttgaaagc atcaacgaca acatcaaaag atgcaagatc agtagttgtc aactcaaata
    14281 aatccttttg tataaacgga acatctgatg gcaatttttc tggatgacgc acgattgccg
    14341 tcacgtctaa atctctggac aatgcttcgt ctaaaataaa tgaacctgca tggccagtag
    14401 ctccaatgag tgcaattttc ataaaaaacc tcctttttta ttcctgtcca ttgtaagcag
    14461 ttgctgaata ttcgtcaaag aatccgattt ttgaaaagct cagtgcgcat tcctttcatg
    14521 gttcgtgatc attgggtata gttaaattaa gaaaactgat tgataaaaat agaagttgaa
    14581 ggagataaga aatgaaagag tggttagatc gtgccttttt accaatgata acatctgttg
    14641 tgaatcttag acccttaact gctttgcgta atggaatgac ttacacgatc ccaataatta
    14701 ttgttggctc gctgtttttc ttatttgctc attttcctgt ggattacatc gctcagtgga
    14761 tagacaaaac aggattttct atgtatttta cacaagtata ccaggctaca tttgatgtga
    14821 tagccttttg gatcgttttt gccattgcat acgcttatgc aaaagaagaa gacatcgatc
    14881 gcttaccagt cggcataaca gccttgtctg cttatctact gcttattcag ccactaaata
    14941 ctcagataga tactgtctgg acaggtgctg aagggatact aggagcagtt acaactgggc
    15001 tcatcatcgg atggggctat gcacgtttag taagacttgc gaaagggtgg aagcaaagag
    15061 agaagatacc tgaaaatgtt tggcagtctt ttacttcatt gattccaatc attgttatag
    15121 tcacttttac gcttatcggt tcagcggttt ttcaacattt gttcagaatg actgttgttc
    15181 aaggagtatg gacatatgtc cagcttccca ttcaatcgtt catagacacg ttgtttggtg
    15241 tgcttttatt aggttttttt gttccgtttt tatggttatt tggtcttcat ggatctgcga
    15301 tggtcaatgg gttggttagt cccgtcttgc aagcgaattc attagcaaat gcagaaatct
    15361 tagcctcagg acaagagttg acggtggcaa atggcggaca tatcgttact cagcaatttc
    15421 tcgatcagtt catgacggtt acgggtgctg gattgacgtt aggggctgta ttttttatga
    15481 cggtttttgc gaaatcgaag aaatacagag aattaggaaa tcttgctctt ttaccagcct
    15541 ttttcaacat caatgaatca attatttttt ccacaccgat cgtgatgaac ccgatgatgg
    15601 ctgttccttt tattttcgca ccggtcgttt caggattgat tacctattcc gctttatatt
    15661 ttggattcgt tcctttattt acagcagtcc aagtcccttg gacaacaccg ccgatcctct
    15721 cgggtttctt agtaggcgga gtgcctgcag cgattttaca aggtatcgtg ttgtcaatta
    15781 gttttttcat ttatttccca tttttgaaaa aaatagattc tgcaaactgc aaaaaggaaa
    15841 gacagacaaa aaatgatgga gcagcagaaa agatgaacga ctaaaaaaca aaaatactcc
    15901 tgtcgcttta gattgggaaa atcaccaatc taggatgaca ggagtattta tagtcatgtt
    15961 tatactagcc ctttttggac agctaattga acaagaacaa ctaatgtgtt cattcctacg
    16021 tgtgtgatca tcgatgtcca gatccgacca gttgctttgt actctaggct gaagaagaaa
    16081 cctaggaaaa agtagattag cagatgacca tcattatgag caaacgcaaa cagcaaagag
    16141 ctgatcactg ctccgaccca aaaatttgaa tatcgactga tgatccccag tatactgcgg
    16201 cggaatacga actcttccat gatgggtcca gcaacagttg tcgccaaaat aaaagcgggt
    16261 gcttccatga tcatctggat gatattttgt gtattctcgg aaggggttgc tttgccaaac
    16321 aatacagatt ctaacatgac agcgatgctt tgcagaatca atgcgacaaa gattccaacc
    16381 aatccataaa taacaatctt tggagcagaa gcttttttta ccgctgattc gatcgctaag
    16441 ggttcacgtt gcttaaaata aaaaatagct aggacaacgg cgcctagtat atacatgact
    16501 gcagttgctg tgatcaattc agacgtagat tttacaagtc ctatcgaagc taggatcaag
    16561 ggtgagataa aaattagacc atagcaaaaa atgctgagga agctatattt ttttaaagac
    16621 atgtaattcc tccttttaag ggataacaag catgcaaggc aagcttatat cgattgattt
    16681 caattatatc ataatttcaa taagcagctg tcagaataaa atttcttttc tttgatttaa
    16741 aagcttgcaa aaaagaggga gaatgagtat tattaaaaat gtaggttagc actcgaaggt
    16801 taagagtgct aacagattaa aaaaagagtt atcggaggga tttatcgtgt taaaaccatt
    16861 aggtgatcgc gtcattatcg aagtcgcaaa agaagaagaa aaaactgtcg gaggaatcgt
    16921 tttagcttct gctgctaaag agaaacccca aactgggaca gttgttgcag tcggtgaagg
    16981 ccgtttgtta gaaaacggtg aaaaagttcc tgccgctgta aaagcaggcg accaagttat
    17041 gttcgaaaaa tacgccggta cagaagtaaa atacgaagga aaagaatatc tgatcgtcgc
    17101 aggaaaagat atcatggcga ttgttgagta aatttggaag ataaaaaaat aatcaaaaat
    17161 aatcaatgag gtgaatcaat catggcaaaa gaattgaaat ttgcagaaga cgcacgcgca
    17221 gctatgttac gcggggtaga taaattagca gatacagtga aagtaacatt aggacctaaa
    17281 ggccgaaatg tcgtactaga aaaatcttac ggctctccat tgatcacaaa tgatggtgtg
    17341 acgatcgcaa aagaaatcga attagaagat cattttgaaa acatgggtgc aaaacttgta
    17401 tcagaagtag catcaaaaac aaatgatatt gcaggagacg gtacaacgac agcaactgtt
    17461 ctgacacaag caatcgttcg cgaaggttta aaaaacgtaa ctgcaggtgc taatcctcta
    17521 ggcattcgtc gcgggatcga attggcaaca aaagcagcag tcgaagaatt gcataatatc
    17581 tcaactgttg ttgattcaaa agaagccatt gcacaagtag cagctgtttc ttcgggttct
    17641 gataaagttg gacatttgat tgctgatgca atggaaaaag ttggaaacga cggtgtcatc
    17701 acaatcgaag aatcaaaagg gatcgaaaca gaactagatg ttgtagaagg tatgcagttt
    17761 gatcgtggtt acttatcaca atatatggta acagataacg acaaaatgga agctgttcta
    17821 gaaaacccat atatcttgat cacagacaaa aaaatctcta acatccaaga tattctgcca
    17881 ttgttagaac aaatcttgca acaatcacgt ccattgttga ttattgctga cgatgtagat
    17941 ggtgaagcat tgccaactct tgtattgaac aaaatccgtg gaacattcaa cgtagtagct
    18001 gtaaaagctc ctggtttcgg cgatcgtcgt aaagcaatgc ttgaagatat cgctattttg
    18061 acaggtggta cagtaattac tgacgatcta ggtttagaat tgaaagatgc aacaatcgaa
    18121 aacttaggaa atgcttctaa agttgttgta gataaagaca atacaacgat cgtagaaggt
    18181 tctggagaaa aagaagcaat cgaagctcgt gtgcaattga tcaaaaacca aattgctgaa
    18241 acaacttctg attttgaccg cgaaaaatta caagaacgct tagctaaatt agcaggtggt
    18301 gtagctgttg ttaaagttgg tgcagctact gaaacagaat tgaaagaatt aaaattacgc
    18361 attgaagatg cattgaatgc tacacgtgcc gcagtagaag aaggaatggt ctcaggtggt
    18421 ggtacagctc tagtgaacgt tatcagcaaa gtttctgctg tagaagcaga aggagacgtg
    18481 gcaacaggta tcaagatcgt tgttcgtgct ttagaagaac caattcgtca aatcgctgaa
    18541 aacgctggtt atgaaggatc agtgattgtt gataaattga agaacgtgga actaggcact
    18601 ggtttcaatg ctgctactgg agaatgggta aacatggttg aagcaggtat cgtagaccca
    18661 actaaagtaa cacgttctgc cttgcaaaat gccgcttccg tttctgcctt gttattaacg
    18721 acagaagcag tcgttgcaga taaaccagaa ccagcagcgc cagcagctcc tgcaatggat
    18781 ccatcaatga tgggcggtat gatgtaagaa aaagcaactt gcttatctac aactgatcat
    18841 tagtgaaaga cagataaaaa gaggctggaa cagaagcgtt taactccaag aaataagaag
    18901 aaattcacgg aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggagttg
    18961 cttctgcttc cgctgtttat acgtgtttag agcgtgagac aaaagtg
//
LOCUS       DAZKOW010000055        18826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000055 DAZKOW010000000
VERSION     DAZKOW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.055"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..361
                     /locus_tag="ACG912_001786"
     CDS             44..361
                     /locus_tag="ACG912_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0513099.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            354..962
                     /locus_tag="ACG912_001787"
     CDS             354..962
                     /locus_tag="ACG912_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFQ0513100.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            1017..1808
                     /locus_tag="ACG912_001788"
     CDS             1017..1808
                     /locus_tag="ACG912_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFQ0513101.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            1946..2434
                     /locus_tag="ACG912_001789"
     CDS             1946..2434
                     /locus_tag="ACG912_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFQ0513102.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            2485..3357
                     /locus_tag="ACG912_001790"
     CDS             2485..3357
                     /locus_tag="ACG912_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFQ0513103.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            3533..3895
                     /locus_tag="ACG912_001791"
     CDS             3533..3895
                     /locus_tag="ACG912_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0513104.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            3904..4656
                     /locus_tag="ACG912_001792"
     CDS             3904..4656
                     /locus_tag="ACG912_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513105.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            4670..6472
                     /locus_tag="ACG912_001793"
     CDS             4670..6472
                     /locus_tag="ACG912_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513106.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            6749..7789
                     /locus_tag="ACG912_001794"
     CDS             6749..7789
                     /locus_tag="ACG912_001794"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0513107.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(7841..9985)
                     /locus_tag="ACG912_001795"
     CDS             complement(7841..9985)
                     /locus_tag="ACG912_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0513108.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(10342..11715)
                     /locus_tag="ACG912_001796"
     CDS             complement(10342..11715)
                     /locus_tag="ACG912_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0513109.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(11715..12110)
                     /gene="rbsD"
                     /locus_tag="ACG912_001797"
     CDS             complement(11715..12110)
                     /gene="rbsD"
                     /locus_tag="ACG912_001797"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFQ0513110.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(12138..13055)
                     /gene="rbsK"
                     /locus_tag="ACG912_001798"
     CDS             complement(12138..13055)
                     /gene="rbsK"
                     /locus_tag="ACG912_001798"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFQ0513111.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(13163..14176)
                     /locus_tag="ACG912_001799"
     CDS             complement(13163..14176)
                     /locus_tag="ACG912_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFQ0513112.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(14257..14856)
                     /locus_tag="ACG912_001800"
     CDS             complement(14257..14856)
                     /locus_tag="ACG912_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0513113.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            15042..15743
                     /locus_tag="ACG912_001801"
     CDS             15042..15743
                     /locus_tag="ACG912_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513114.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            15753..18464
                     /locus_tag="ACG912_001802"
     CDS             15753..18464
                     /locus_tag="ACG912_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFQ0513115.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
BASE COUNT         5943 a         3382 c         3859 g         5642 t
ORIGIN      
        1 agattgaaat aaaatattac acttgagaat agaaggtgac gtaatgcaca tccagattcc
       61 aactgttctt cttgatggta ctgtgctagc agcattaagc aaggaagata tgtatgggta
      121 tgccttaaca aaaatcgtac aagaaagtct ctctgtcagt gaatctacac tatatccggt
      181 tttgagaaga ttgaaaaaag aaggatatct tgagacctat gatgaacctt ttgaaggcag
      241 aaaccgtcga tattactcgt tgacaccaat gggaagtcgt catttagaag aaattattga
      301 tgaatggatt gtttttcgag attctttaga ccggttattg gaggagagca caaatgaatg
      361 aatatttgaa tgaagttgtg gatcatcttc aagaaattcc tgaagacgat cgttttgatt
      421 tgatccagta ttatgaggaa tattttttag attctggaaa aacattagat cagatcattg
      481 atgaatatgg tacaccaaag cagtttgcct taaaattaaa aatcagttac ttttcagaag
      541 aagaacaagc agaaaacgag ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt
      601 taatcattat cggtttattt gcttctcctg tcctcattcc actagcactg gggtttatat
      661 tggttattgg gggactattg gttgctttac tagcgctcat ttttagtatc tatgttttat
      721 gtatcagtat cttaggtgtt ggcctattca tgatcatctc aggaatcagt gtgtttggac
      781 aatcgattct gagtggatta ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag
      841 tatttatttc gccactgttg ctgaaaggaa caaaagggct tttttatgtc atgatgaact
      901 ttgttaaatg gatgggacga cgttttatta caaaaagaga tctgcattca gcgggcgttt
      961 gaaggaggac acataaattg agattgaaac attttttgat taccggtatt ggtctgatgc
     1021 ttactggtgg cctcctagca ggtgcaactt atacattagg cgctcaaaag agtctgactt
     1081 gggataatgg tcctcggttg atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa
     1141 acaaaatcgt catccatgca gaaaatcaaa cagtttcggt catacgcgga ttggattttg
     1201 agataaaaac gacttatgat aagacagaaa agccagaaat aaaggagaaa aatggcgtgt
     1261 tgactgtgaa tgcagtcaaa aaatcaacag gtgcaatcat tagtttagaa gacacaagtg
     1321 cacaaatcac tattgttgtc cctgcagatc atgaattatc tgaattgaca atcgaaggga
     1381 ataatagtgc aattgatatc aacgaagtat caatcaaaaa agtgaatact aagacacatg
     1441 atggctgggt atcgttcaat gaatttacag gaggaacaat aactgcgaaa gatgaattag
     1501 gctcacttcg attagatgag gccaatgtgg ataacttgat cgtcacaggt gaaaacagca
     1561 acttccatct agaagacagt ttgatcagca aagaaggaaa agtcatccta aacggtggca
     1621 atttatcttt gcatgagatg gaaaacagtg gctatactat ccaaacgtct ggtgaatctt
     1681 atattgaaaa agactaccag ccaataacaa atactttgtt agaacaagga cagaaaaagc
     1741 taaagatcac tgctaaagac acatcgatca atattacgac ggatcaagag gacaatgaaa
     1801 cagattgatc ctgtactcaa ctggatattt taagaaaaaa cagcaaagat ccttccaatc
     1861 tttgctgttt ttcatcgctt aacgcacata actatagtaa aataagaaag aaaatatcta
     1921 aaaagaaaac agggtgaaag agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa
     1981 aacagaaatg gaaagattac atacttatat cactcaacta agccaaacct tctatgatcc
     2041 agacaaagaa aaagtgatgg ttaattaccc gaacaattca gaaggtcgcc aattagagca
     2101 agtgtaccat gaagttttca aacatttatt gacggtgaag aaagagctgg attattattc
     2161 tttaccgatt atcgatacag gaatattgaa gtatgatggg aagaaggaac gatttatctt
     2221 taaatctgtt cgggaagatt tgcctctttc agcagggatg gatctagagg tcttggtaga
     2281 agactatttt acagaagaaa agcactgggt tcgaacaaaa ttggattatt taccgcaggc
     2341 agccggaggg acacaggcaa gcggttggta catcactgaa gacaaagagt tagaactaga
     2401 aggtgtaatg gcgcgtattc gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa
     2461 ggataaagga gcgcaggaag tagcatgaga gaaaaaagag cattttatat caatggttat
     2521 gcagggattg tcggattggt cgtattagct ttagttggat tgtttttctt ctatttaggt
     2581 atgtggcaag cgaaagtttg ggcacttttt ttaagtattt ttctttggtt gatcacgtta
     2641 ttgttgttaa gttcggcaac tgtagtcagt cctaatcaag caaaagtaat tttgtttttt
     2701 ggacaatatt taggtacgat tcgagaaaat ggctttttcc ttacgatacc tttggcacaa
     2761 aaaatgactg tttcgttgaa agttcggaat ttcaatagtt ctgttttaaa agtcaatgat
     2821 ttggatggaa accccatcga aatctcagca gtagtagtct ttaaagtcgt tgatacagca
     2881 aaggcattat tcgatgtagc gtattatcaa gattttgtag aaatccaaag tgagacagcc
     2941 attcggcata tcgctagcca atacccgtat gatacattca atgagaatga tttgacgctt
     3001 cgaggaaaca cgacggcagt gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca
     3061 gtagcaggtg tcgaagtaat cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc
     3121 gctagtgcca tgctccaacg tcaacaagca aaagccattt tgtctgctcg acaaaccatt
     3181 gttgaaggag ctgtcacaat cactcaaatg gccttagagc aaatcgaaga tggacaagaa
     3241 atcaatttca ccgatgatcg gaaagtccaa ttgatcaata atttacttgt ctcaatcatt
     3301 acagacaaag gaacgcaacc tgttatcaat acaggcgaca tgaaagaccg taagtgacat
     3361 actttttgta agataggaag aaggagttta ggacggatta ttgtcttgaa ctcttttttt
     3421 agttgtttta aagaaaatga caaaattgaa agaagaatga tagttgattc gaaggcagaa
     3481 ttgttcgctt ttttctataa tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa
     3541 acttctgatc ggtctacttg ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga
     3601 ttacgtagtt atgggtggag aagaatacta tgtacaaatt acgacagatg gtacaaaaac
     3661 agtttcaaaa tcagactctg gacaagaaac agtcaactat agatatatcc tgcctggcta
     3721 tacgaaagag ggtaaggaaa aagaactgga cttcaaggga caattgactc gaccgcttcg
     3781 taaaggagca tatttaaaaa tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga
     3841 agtcgaaaaa aaagacgtgc cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa
     3901 gaaatgaaca aaatagtcga agtacagaat gtaacaaaag tatacggaaa aaacaatgag
     3961 aaaaagacgc aagcattaag cggcatcagc ttcgatgtag aaaaaggcga atttattggg
     4021 atcatgggcg cttctggttc tggtaagtcg acacttttga atattttatc cacattagat
     4081 aaaccaacag atgggcatat ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat
     4141 caactagcag attttcgtgc gaatgagatt ggctttattt tccaagattt caatttatta
     4201 gaaagcttga cagcacaaga aaatatcgct gttcctcttt ctttacaagg cgtcagacca
     4261 aaagaaatca agcaacgtgt ccaaaaaatt gcagaacgtt tatccatctc tcatattttg
     4321 gaaagctatc cttctgagat ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca
     4381 ttgatcacac aaccaacgat tctattaggg gatgagccta caggtgcgct ggattccaaa
     4441 agtgcccggg atcttttaga tacgatggac gaattgaata caaaagatca tgtatcgatt
     4501 cttcttgtga cgcatgatcc gttttctgca agttattgcc aacgtatttt attcatcaag
     4561 gatggcggta tccatcaaga agtgaaacgt ggagatcaaa gccgtgattc cttctatcga
     4621 gaaatactat ctattcttgg aaacttggaa cagtaggggg agattgacta tgctttggaa
     4681 attatcactt actgggatca agagccgttt aagagactac ttggtgttat tctcaggatt
     4741 agttatggca tctgctattt tttacatgtt tgaatcaatg gcaagcaacg aagctttttt
     4801 gaaaagtaat acgatcgttg cggctacagt tatcattttc cgattcggtt ctgttttact
     4861 agggatcatc acattcgttt atattttata tgcaaattcc tttttgatga cgatgcggca
     4921 aaaagactat gcaatgttta tgatgctggg agcaaaaggc agaaaaatcg cacagatgat
     4981 tttttctgaa acattcttag tagggatcgc agctacttta gtgggttcag ctgtcggtgt
     5041 aggattgaca agtatcgtcc accgattatt agtggatcag ttagatctca atatttcgca
     5101 ttttacacct tttagtattc acgggttgtt gatcactgtt ttgttttttg ctgttttgtt
     5161 tttattagct gctgttgtca atgctttttc gattgtcaaa aaaccgattt tagcattgat
     5221 tcgtgcggat cagacaccaa caagaatcaa gcagcataaa ttcttgtttt tccttgaagt
     5281 agtactggga attgcttttc taggctgcgg ttactatatg atgaaacacg tggggaccta
     5341 tcaagtcttt ggtatagggg tagccttagt tacgatcgta ttaggaactt atttcatttt
     5401 ccattctgtg atcattttct ttttatcatt actaaagaaa tcagaacaga tttcattgaa
     5461 aaaattgaac aatttcacat tgtcacagtt aaactttcgt attcgtgaat atacacaaat
     5521 gctctcaatg gtcgctatgc tgtttgcctt ggcactgggc gctttgacgg taggattagg
     5581 ttttaaaaat gaaattgcag acttgacaga aaacaattct ggatatgatc tagtgatgaa
     5641 taatcctcgg gcagaagatc agcaaaaaat caaagaactt tcaccaacac tttctgcttc
     5701 ttatactcaa aaagaagatg cgggaacgat ttactataat gcaagtgaat ttgcggctca
     5761 accattgatc atgataaaac atgaagatgg aacaccgccg aagatcacga aagaaaaagt
     5821 tcctgctaaa aaaatgaatg aactgactgt acaagaggaa cttcgaagct atgaattgcc
     5881 ggaacagcaa gaaaaagaaa tcaaggtagt cagtcaatca gaatttgatc aactgaactt
     5941 gccacaatcc actttacagc tagttcaggt aaaagacttc caagcaaacc tgaaaccaat
     6001 cgaaacactt gttgctcaaa ataaaatgaa caatccatct ttgcaaaaag tagagtacag
     6061 caaccagaaa tatgaaatgt atgaagtgta taatggtcta ttctctggtt ttgaatttat
     6121 gggattcttc ctaggtttag ctttcttgac catgcttgct agctgtttga tgttcaagat
     6181 cttgtcagga agcaagagtg acattgctcg ttatgaaatg cttgaaaaga taggaacacg
     6241 acgcgggttg cttaaacaat cgatccgtag agaagtaggc gtgttattct tagcaccagg
     6301 aatcttaggt gtagtccatg tcttattcgg attacaaatg tttggtctat tcatgcaaaa
     6361 tccatacaaa gatatttggg ttccgtttac gatattcttc gtattgtatt tcatctacta
     6421 tgtactgacg acatggttat atactggaat cgtattgaaa aagagattgt gaaaaagctg
     6481 tcctgcatca agtaataaga acagccaagc aaaaatagct cctcatattt ttcgtttggt
     6541 tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaaaa agaggttgtg
     6601 acaatatttt tgtcacaacc tttttttcac ggtcttttat tagaatatag taaatttttt
     6661 ctcgttactc acggaataag ctgttaagaa aacggtttcc atgtatactg gaatcagtgc
     6721 aaatgaaaga aaagagggtg caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa
     6781 ctatcacttg attacgatca cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg
     6841 ggcacgggtt gttcattttc aaacaataat cgataatgaa aaacgagagt tgatcttagg
     6901 ttttgatgaa gcaaaagaat atctagaaaa agacccttat atcggtgctt caatcggacg
     6961 tatggctgga cggattacga atggcacatt caaattagat gatgaaacgt tcgaagtaaa
     7021 aacagacccg gacactggtc attgtttaca tggtggtgca cctagctttg aagccaagca
     7081 gtggcattat acagtcatca atggtgaaaa cgaagcatcg gtcattttct atcagactag
     7141 cccagatatg gaaaacggat ttcctggaac tttagatgta gaagtacggt atacgctgac
     7201 gaacgatgat acatggcgag tgacggtcca agggatcagt gaccgcaaaa cagtattcaa
     7261 tcctacaaat catgtctatt tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca
     7321 tatactttgg ttgaatagcc gattatttgc tacgctcaat aaagatacaa cgcctacagg
     7381 agaagtagtt tcagtaagtg gcacagcatt tgacttccaa cgtcctaaaa aattaggtga
     7441 ggtctttgcc agtgattttc gccaatcaat gatagtgggc ggcatcgatc atccattttt
     7501 gttgaaaaaa caatctggtt tactaaatgc tgcggaattg acaagcccag atgaaaaagt
     7561 atcgatccaa gtaaaaacag atgcttcagg aatcgtgatt tttacagcaa attttggaga
     7621 agatggaccg gaaatgcgcg ggaataaatt agtaaatcat ggcggtatca catttgagac
     7681 tcaagaactg ccaggagcgg agcgacttga aagttttgga gatgtcactc tggaacctaa
     7741 ccaagtacgc gagtttgtaa cggaatacaa aataaatata aagaaataga ataaattaaa
     7801 aagggagtgt gacagtgtca catccccttt ttaatttatt ctatttcttt aaaggcagat
     7861 caagccagaa tgatttgtct aaacttaaca aggttagtac aggaacagtt gcagctttaa
     7921 ctgtacgagt agttgtttct ggtatctgtc ttgccgtaaa attgttccac aaatcccaga
     7981 ttgaagcgac acggaagatg gaagaagcgt tgttgacgcg ttttatcgtt gaaaattcta
     8041 atcctgtgat tggcacgcca tctgttgtcc atttgattgg cacatcgaat cgcttagaca
     8101 tattgaggat ttcagcgttt gcttcttcag ttgttgctgc ttcttttttc ggttcatcat
     8161 attttgtcag gtcgtacgct tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg
     8221 tagcataacg tccttccaaa tatttggttg cttctttgta gtcttttgtt tccgacttcc
     8281 aagttggttt gtagaaatca ggattgccag tcagtccatt tttgatcaag tcggcatagt
     8341 cttccaaact ttccttgtaa gaaggatatt tacggaattg tgcgcggatt tggtagcttt
     8401 gtccggaact atcttgttca agtgtattga aggtcacgct gcttccttta tatgatcctt
     8461 tgataccaaa aaggttatga tttggttcgc ttgataataa gctgtttcca gatccacttt
     8521 caaggatagc ttgagccatc atgactgatg cgtagattcc ttctttttgc ccgatttcct
     8581 gtgcatcatc tgcaatcttt ttcaagaact gatcagttgt ttggttttga ctgaaatgga
     8641 acgagccatc taatccatct aaaatagatg agccgcctgt cagcgaacct gaagcagcag
     8701 gtaaacctgc taagtttact cggacagcaa aatcaggcgt gaaatctgaa atagaagtta
     8761 ctcgaacttt atctcctggt tgaggcgcat ggataaattg tccgttccca atatagatac
     8821 ctacatggtg agtaacaccg gaagaaggtc cccagaagac gaggtcgcca ggttgggcct
     8881 gacttactgg aatctgcgta cctgcatatt gctgttcgcc tgtccagcca ccgatataat
     8941 gaccggttgc tttcatatat acgtaataag tcaaaccaga acagtcaaaa gtatttggac
     9001 cttttgctcc ccatacatat ggttttccaa gatgcttgaa tgcttctcga acgattgctt
     9061 gttggacatt cgttgtattt gtaggcaata cagcatcaat tggtgatttt acaacaaaat
     9121 cagaagaagc agaagatgga ttctctatac cgctttgatt tgctgtattt ggacgagatg
     9181 aagcattgtt cgttccgcca ttcgatgaac cattgtttgg atggtttggt tctctctgag
     9241 tatttccatt gttattcgat ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg
     9301 aattattgtt attattgttt gaattatttg aaccattatt attgttgtta ttacccacat
     9361 ctggtttgtt tggcgtttcc ggatctggtg tactagggac ttcttcacta ggatcagttg
     9421 gtttctctgt tgaagtatcc ggtccagctt taaattctgc agtaatagat aaattgttcg
     9481 tacctactgt aaaggtcttt gcatcataat ttacttcttt ggcaccatta acagcgactg
     9541 aagcaatttg ttcaccttca gctggcgtaa tagtgtaagt taccttcgtt tccgctaata
     9601 aaccttctac cgttccactt tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg
     9661 tcgaagcagt cagttggatc ttaccaatca aagaaggagc aattgtaagg gaatatgtct
     9721 cggctggctt ttcattttct gatggatcag ccggatcaat ttcagttggc tcggtcgatc
     9781 ctgttgttga gttagttgtt ccagttgggt cttctgtaaa agaatccgtt gtttcactag
     9841 tagctgagct ggatgtagtc gtatctgttg tgactgtttg atcagctatt tgattatcta
     9901 cggcaaatac ggtattggtc gacaaaacag acggagcaat cagccaagta gcagctagca
     9961 aacgtatcgt ttttttattt ttcataataa actagtcctt ttcctttcat tgattttctg
    10021 tcttattcag cattcattag acattttttg gaacagataa tcgtgtcgaa aagctttttc
    10081 ttttcataaa tctgtctgtg tattctaaat aaaacaattt gtaaataagc aacgagcatt
    10141 cattcgtttt tctttgacgt tcattttatt gcatagacca cctttgtttg gggataaaca
    10201 agtggggata aaaatggtta tgcatatgta aattatacca aagtttggaa agtatcacaa
    10261 aaatttataa aaataatgat cctaaaaaga aaaagactga aatatgtgat aaacatctca
    10321 tacttcagtc tttttcatta actattcata agacattagg tcttcatcaa acccgctaac
    10381 gagagtagtg atcattgcta cgactagact aacagccagt cctgctaagt accagaaaaa
    10441 agtaccggaa atgaatgcag gcaatgtggt cagactacca aatacaaaag cgatcgcttt
    10501 cacttgggca aatcccatta cagctccacc agcagctgca ccaatgcaag cagcaacaaa
    10561 tggttttttc aatgggatcg caattccgta aacagttggt tcggtcactc ccgcaagtaa
    10621 aatcgaagca aaagcagaac cggagatcga acgggttttt gaacgtttac ttcgcagaaa
    10681 tacacctaaa gctgcaccag ccattccaaa attggctgct cctgtcaacg gaacgattcg
    10741 gtcgaaccca taagcagaga tattactgat catgacaggg tttacagccc attggatacc
    10801 aaatattacc aaaatattcc aaccaccagc gatgattgcg ccactgatcg gtccgttaat
    10861 atccatcatc caagtaattg cggcagctaa accttcgcca aggtaaacac ccgcaggccc
    10921 gaataccatt aacgtcaaag gaaccgtgat aactaatgaa agtaaaggta caaatactaa
    10981 ttgcatattt tcgcgaatat attttttgag gaatttttca acaaaagaat aaaagaaaat
    11041 acctaaaatc gccggcatga cagtagacgt atactgcatc agtactacag gaatccccag
    11101 aaaatcagta gctgctttgc cactgtctga aagtaaagag gtcagattcg gttcaatcag
    11161 tgctgcagca atgacagccg caatatatcc attcacttta aacgttttgg cagcagaaaa
    11221 agcaagaaag attggcagaa aataaaagac ggcattcgac gcagcagaca aaatatgata
    11281 agttccgctt gtttcactaa gccagccaat cgtagtcgct aaaacaacca gaccttttaa
    11341 tataccagaa ccggcaaaca gcggaatgat cggcgtaaag atttccgata tcaaaccaaa
    11401 tagtttttcg atcggattta gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa
    11461 ttccttgtaa gcaggagcca atccctgatc catgattatt tctgcatatc tatcgagtaa
    11521 aatgaaatac ttaatggaag ggatttgctg ttttaatttt tctgcattgg ctactggctg
    11581 ttttttcaat ttgatacgaa gccgagtcgc acatggaagg acagaatcaa tattgtcttt
    11641 tcctcccaac tgttcaatca gcatatgaat ctgctcggac agtaccatct gttcaggatt
    11701 cattgtcgtt tccattagaa gaccactcca ctcactaata taatattggc gtaaggtgtc
    11761 atttcgcctg tacgaataaa tgcatgacag ttattcaact cttttttcat ttcttcatgt
    11821 gggataaagc taatcggtgt atctggtaac aataatttga ttttttccaa tatttccggg
    11881 ttcattgtct ttatttcttc cgccaattca atcgattgta cctcaagttc ttctaaaacg
    11941 ttttctaaaa cagataaaaa acttggaaga ccgtttgaga cagccaaatc tattttttca
    12001 gtaccaaagg ggacaggcat gcctgcatcc ccgatactta atttatcaaa gtgccccatt
    12061 tgtgcgatta ctcgtgaaat atctgaattg attacttttc cttttttcat gtttgtttct
    12121 tcctctcatt gttaatttta gactgctgat tgttctaaag cttcgcggta agggatagaa
    12181 ggttgcgcac cataagactg cacagcaaca gaagaggcta gatttccata aaggatcgct
    12241 tctttcaagt tactaaggtc tggctgaagt gtgctgctta atgcgccaat aaatgtatca
    12301 cctgcggctg ttgtgtcaac ggcatccact ttaaaagcag ggacgatacc gtgttctttt
    12361 tctgtatgat agaaagcgcc ggcactgcct agtgtaatga ttactgttcc aatacctaat
    12421 tcatggagtt tttcggctgc tgcgaccaaa ctgttatgat ctgtcactct gattcctgta
    12481 atgatttctg tttctgtttc atttggtata atgatatctg tcttttcaag tagttcagta
    12541 ggaatcgttt cacgagctgg tgcaggattc aggatcgttg tttttcctgc gtcttttgca
    12601 atagaaaaag ctaggatcgt cgcatctaag ctactttcaa actgagcaat aataaagtca
    12661 ctgtttgtaa tcagttgctt gttttttagg acttcttgtt catggaaagc attatttgcc
    12721 cctgcatgga tcaagatact attttctcct gctgcgtcta ccataatcat tgcttgtccg
    12781 gttgtggcgc cttggatttc agctatccca gatgtatcga tgttttcttt tgtcagtaga
    12841 tctaataact gttgtccttc gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg
    12901 cctgaacgtt tggctgccac tgcttggttg gcacctttac ctccgccagc gtgaaaaatc
    12961 tcatgcgcat gcatcgtttc gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc
    13021 atattaatac ttccaattac tgtaaccgtg ttcattttac aaacctccaa ctatctcatt
    13081 tattttgatg ggaaaattat tttttaaaac gttttagtaa aacgttttaa tagttcgata
    13141 aaaatatagc acaccttgaa agctatttca agggtgcagt tgaaaatctt ttttctaatc
    13201 gtacaggtaa tcttttttct tcccattctt tttctgggaa ttggatccga tctaacaata
    13261 acttggcaga tgtttgtccc aattcgaaga ttggttgtgc gatcgttgtc agtttcggct
    13321 tgatatactc gcacatatct atattatcat agccaataat gctgtagtct tcagggatgc
    13381 tttttccagc ttcctctaat ccgcgatata atccaaaagc taattcatca tttaaagcaa
    13441 acacaccagt tgcagcagaa tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg
    13501 agaactgtgt aggtaagagg atcagttggt cgtgagggat ttggtaaaca tcaagagcag
    13561 atttaaaccc ctcaatccga gcgtggacat tttccggtgg attttcagga tagacaaggg
    13621 cgatcgtttg atgtcctaga ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat
    13681 cgtctgttcg tacggcatca ctaaatcctt cagatttttt ctgatccaaa acaatgaagg
    13741 gccggccttg ttttttcaaa ttttcgttga ttgcatctgt agagacagca gaagtcgcga
    13801 taatgaatcc atccacacct cgacgtgtga gctcggctag atattcgatt tctttttgat
    13861 gatcagaatc agcattacat aatatcgtga cgaaattctg cttatacaag atatcctcaa
    13921 ttcctcgcat cagtgtactg aagaatggat tcgtgatatc aggaacgagt acaccaattg
    13981 ttttcgtttc cttggtaatc atttgtctag caaaatagtc aggctgatag cctaattctt
    14041 ctttggcagc taaaactttc tgaacagttt tcggactgaa tttctccgta tttccattta
    14101 atatcaagga gacagttgtg attgaaacgc cggaaagttt agcgacttct ttgattgtga
    14161 tctttttttt caccatgttc atactcctat ctaaatcctg tggttatttt cccatgaaat
    14221 caaaaagaag taaatgaaaa tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc
    14281 gattatttgt ttcgttgagc gtactcgtgc agttttcgga aatatctttt ttatgaacaa
    14341 ttgatgggat tctttgtcac gatcactgca agcatcttca gcaagcacga tatggtaccc
    14401 gtgttgaaag gcatctaatg cagtagcata tacaccgttt gttgtcgcta caccgcttaa
    14461 aatgatagtt tcgattccac gccggcgaag ttgaagatca agagaagtac caaaaaaagc
    14521 acttggattg tattttgtga tagtcaacat attatctgta tctttcaaag aatccttcaa
    14581 aagtaaatca gtgaattctg gaggtacaga agcagtcatt ccacccatat cagtttgttg
    14641 actcaaataa gggaaagtgg agatgtctac gttcactaaa actttcaagg cagcggtacg
    14701 tttcaatgtt cttactaaca aatcattgtt tgtcagaata gatgctgcag tgtgtggaga
    14761 gagtgtaccg ttatttacga tctctttttg catgtcgatc gcgacaaaag cagtttttga
    14821 taaatcaaaa atcatatttt cctttaaatc attcatgatg ttccttcttt caaataacaa
    14881 tgttttttat gtaagtaaat cataaatcgt ttatttgtca actaaattaa aggattcctt
    14941 tctttagtct agagaaattc agtggtaaaa taaataagtg tgagaaaacg cattcgatgc
    15001 atagatatag cgagaaatat cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa
    15061 aaaatgaata caaacgatac aaaatgggag aaacgaccat tacagcaaat gacgatattt
    15121 ctttttcaat tgaaaaaggc gaattagtga ttatacttgg tccaagtgga gcaggaaagt
    15181 caacgattct taatatttta ggtgggatgg atacacctga tgaaggtcag atcattattg
    15241 atgacacaga tattgcgcaa ttttcagata aacagcttac tgcttaccga cgaacagatg
    15301 tagggttcgt tttccaattc tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg
    15361 aattggcaac ggaagtttct cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg
    15421 gcctagctca tcgattgaat aactttccgt cccaattatc aggaggcgaa cagcagaggg
    15481 tttcaattgc tcgtgcacta gctaaaaatc ccaaacttct tttatgtgat gaaccgacag
    15541 gcgcgctgga ttttgaaacg ggcaagcaag tattgaaatt attgcaaaac gctagcagac
    15601 aacacggcaa taccgttttg atcattacac ataattctgc tttagcgccc atcgctgata
    15661 gagtcattca cattaatgat gctaaagtaa ggtctgttga attaaatgat catccttcat
    15721 ccattgatga gattgtatgg tagggggatt ttatgaaaac aagaacatat cttaaagcag
    15781 gttttcgcga aattcggcaa tctaaaggac gattcattgc aatcatcctt attgtcatgc
    15841 taggaacact tttatttgta ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg
    15901 atcactatgt tggtacagca ggactttctg atttgcaaat cgtttctact ggtggcttga
    15961 cacaaaaaga tattgctcaa gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac
    16021 agatctatta ttccaatact ggcaaaaacg aagtgattca aatattttct tataacaaaa
    16081 ataacaagca gaacaagcta caattaactg atggaaaact cccggaaaaa ccaaaccagc
    16141 ttgtattgga tgaaaaagcg gaagatgaag ggtataaaat cggtgatatt tatcagattg
    16201 attcagatga attgaaagaa aaagagtata ccatcacggg ctttgtccgt tctccattgt
    16261 tcatcaacaa tttggagaga ggatacgcga acgtcggaaa tggtagcgtc gattattttg
    16321 tctatttacc tgaatccgtt tttaaaacag atatttattc tgtcttatat cttgatttta
    16381 caaacgtaaa gaatttgaat acgtacagca aagcttacaa agataaaatg gagcaaaatc
    16441 aagaaaaggc agaaaagtat ttacaagatc gacccgaaga acgcttagaa gagctgaaaa
    16501 aatcggccaa cgaagaatta gaaccggcac agcaaaaaat agcggatggc aaagcacaaa
    16561 caacgcaagc aagaacacgg ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa
    16621 ctatccagca gcttcctgaa gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag
    16681 aagctcaact tgctgaacaa gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg
    16741 cagaggaaga attgaatcga aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc
    16801 tatacaatga acgttcagaa aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta
    16861 tcgctgcgat tgccaatgtt tttcctgttt tctttttctt tattgccgca ttgatcacct
    16921 ttacgacaat gacacgtatg gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt
    16981 taggttatac gaaagttgaa atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag
    17041 ggataggtat tgttttagga acgatattag gaacgaatct tttgccaagg atcatttttg
    17101 agctgtctaa cgagcggtat gatattggga gtgcgcttat tttttatgac tggccgccga
    17161 ttttacaagc agctgccgca ttcttcattg ccgcatttgg cgctgcaatg atcgttttat
    17221 tcaaagattt aagagaacgg cctgctgcat tgcttcaacc gaaagcaccg aagccaggga
    17281 aacgcatttt tcttgaatac atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa
    17341 tcagttaccg aaatctcttc cgatataagt caagaatgct tatggcgatt atcggaattg
    17401 ctggatgtgc cgggctgatg gttgcaggcg tgggattgaa agattctcta agttctgttt
    17461 cagccaaaca atttggaccg atcattgact atcaagcaat cgttacattg gattcatcca
    17521 cggaagatag tcgtcaaaaa gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga
    17581 tcctagctat tcatactcaa accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc
    17641 tgacagtgat cgtaccaagt aaaacagctg atttggatcg ttttatccat ttaactgatc
    17701 ttgaagagaa cccacgctcc ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag
    17761 aactatttga cttatctaca ggagatcata tttcgcttta tgatgaagat caaaaagaat
    17821 ggaaagtcga agtaaaagaa atcgtgaaaa actatctagg tagctttatt tatatgtccc
    17881 ctgaatatta tgcttcagtt actgatcaat cgatgatgga taatgcattt ttgctgcgta
    17941 cagataagat gacaacaaaa gaagaagaaa tgctttccga gaaactgctt gccacagacg
    18001 ctgtgacaaa tacgtctttc atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga
    18061 acttagactc ggttgtctta atctttgtcg tattatcagg actgctggct tttatcgtct
    18121 tgtataatct aactaatatc aatatttccg agcgtgtacg cgaattatct acaataaaag
    18181 tccttggatt ttttgataaa gaagtcacga tgtacattgt tcgagaaaat atcatcttta
    18241 ctcttttagg aatcatcgga ggatttggtg taggatatat attgactgac ttcatcttgc
    18301 aacaagcgtc tatggagaat gtcatctttc cattagtgat cacatggtgg gcttatgtct
    18361 tatcagcagg attaactatc gtatttacgg ttattgtgat gattgttacc catttcaaat
    18421 taaagcatat cgatatgatt gatgcgttga aatcgaatga ataaaaacag aggttgtgaa
    18481 atcagcggtt tgacctgagt attagagaaa caaacggcaa aatagttttt catattttgc
    18541 cgttttttga gctaataccg aaggacagct gattgaacac catgaatcag aggttgtaaa
    18601 aaagccgtcc tgcatcgagc aaaaagaata ctcaaataaa attagctcct catgttttac
    18661 atttgagaag actgattgcc gtagataaaa gcttttgaac accatttata gaagaaacaa
    18721 gaatattttg ctattttaaa caagaggata gacaaataca tgtctgatct cttgtttttt
    18781 attgggcaac agacctctta gaatgtataa agataaattt tacaaa
//
LOCUS       DAZKOW010000056        18640 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000056 DAZKOW010000000
VERSION     DAZKOW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.056"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..937)
                     /locus_tag="ACG912_001803"
     CDS             complement(26..937)
                     /locus_tag="ACG912_001803"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0513116.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1314..1952)
                     /locus_tag="ACG912_001804"
     CDS             complement(1314..1952)
                     /locus_tag="ACG912_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513117.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDFVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFNQETKHLTGREKRLIKRNALSFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKEQVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(1956..3938)
                     /locus_tag="ACG912_001805"
                     /pseudo
     CDS             complement(1956..3938)
                     /locus_tag="ACG912_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            4118..4783
                     /locus_tag="ACG912_001806"
     CDS             4118..4783
                     /locus_tag="ACG912_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513118.1"
                     /translation="MNIGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHQTLGLPIDFPSIESATESIEPTLPDEPLVIKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETVDRFNPFLKPQIGYTTLPVGYVENSIEHWVCNDIFVHGTW
                     LSFHAGEVEKGKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILIQDYDDALKRSPQEP
                     DRH"
     gene            complement(5180..7213)
                     /gene="brxL"
                     /locus_tag="ACG912_001807"
     CDS             complement(5180..7213)
                     /gene="brxL"
                     /locus_tag="ACG912_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFQ0513119.1"
                     /translation="MEQQEILKLSEFFPGKIVRKDLTKKIKEGANVPVYVLEYLLGMY
                     ASSLDETLVEKGVENVKNILARNYVRPDEAEKVKAKIRELGGYTVIDKVTVHLNEKKD
                     CYEAEFSNLGLKNVQISEEYPKKFDRLLGGGIWCIVSMEYWYDENDRWNSPFIAKKVD
                     PIQMPGLDIEAYKMARKEFTTNEWIDLLLRSIGMEPTQFNKRVKWLHLARLIPLIENN
                     FNLCELGPRGTGKSHVYKEISPNSILVSGGQTTVANLFYNMASRTMGLVGMWDCVAFD
                     EVAGIKFKDNDGVQIMKDYMASGSFARGKEEKNASASMAFVGNINQSVDVILKTSNLF
                     EPFPEVMGTDSAFLDRMHCYLPGWEIPKYRPDFFTNDYGFITDYFSEVMRELRKVSFG
                     DSFDKYFKLGSQLNQRDTIAVRRIVSGMMKLLYPDGRFEKSDVEEILRFALEMRRRVK
                     EQLKKIGGMEFYDVNFSYIDLEEEREEYVSVPEQGGGTLIPEGLGKPGHLYTVSYGKT
                     GQLGTYKLETQMTSGNGKFERTGINSDAGAREAMDSAYRYLRANYGSVSQSISLTTKD
                     FVVHVQDMNGIGMTKTLSLSTIIALCSVSLNKPVLPSLVILGDITIGGSMIKVDNLAN
                     CLQVAKDAGAKKVLVPMISAADIGTVPPELMGTFNLIFYQTPEDAVFKALGVE"
     gene            complement(7331..9853)
                     /gene="pglZ"
                     /locus_tag="ACG912_001808"
     CDS             complement(7331..9853)
                     /gene="pglZ"
                     /locus_tag="ACG912_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFQ0513120.1"
                     /translation="MTDINTQQIEEKLNQRFPNYGCRKIIFWFDSNQDFLEEIDSLVI
                     QNAKIYRLEKDAQFKTKRLLEYEDPETNYLIYAPFPRWDDKDEDNHLLSVLNYSEEFS
                     ADRLAIIMNELAIPLRFHQTVKQYRNFFNAKDRMRSFGEFSTHMTINKSETIELMIMA
                     VLLKSKSIRLNDLLRVVLKSFTESRENPLRELDKYQLTAKFWEFIQMKFGFTDSQPTL
                     EKLTMCLYLNYFYYQIDQEMPKKFKGFEILHLKANIITFMDQFMNDTRYMESFEQLSK
                     KVYQAIDGDGLLDSIEIDDLIEADVFSEIHAKILMFYAERLKASDTTTKIAGQTIFDS
                     LKQREKMHFASDYIYEYQLLKHAYYLVDKNRLCLPMNMDNLLTQYEKEMYWVDTHYRK
                     FIWNSDHIEEKQKYGQLYRLVEANYSKFLDQSGQIWNQTFEFKNRPSIRKFYNQAVMK
                     RSVKTVVIISDAFRYELGMNLERKLVREKKYSTNMASFLSVLPSVTEFGKAALLPNNQ
                     LTYNEGTEVLIDGKKTNGLSNRNAILQQRDANAVALNYDDVVKMNKAEVREVFNGKSV
                     IYIYHDQVDRTGDHSNENRVFEACDLAISEISRLINRLHNDGSIYRFIVTADHGFIYK
                     RSHVEEVDKIENPSRNEKDRVERRFILSEEEYEIIGTKNYSLGDSLGNDDTRNICVPI
                     TSSIFKKAGGGQSYVHGGSSIQEMLVPVLEITVAKGSNAKEPVAIELMTSNRRITGLS
                     TTLEFYQKNAISDVYDPASFWLYFEDEYGERISNEEIYVADSKTEDANQRFTKFVFEF
                     VNRRYTVEDTYYLVIKNQKNDLTERIKFIIDNPFAESFDFDI"
     gene            complement(9969..13601)
                     /gene="pglX"
                     /locus_tag="ACG912_001809"
     CDS             complement(9969..13601)
                     /gene="pglX"
                     /locus_tag="ACG912_001809"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFQ0513121.1"
                     /translation="MDKKAIKNFAVNARRKLIDEVMTKAKRLGIDESKIEDVKKIDSN
                     LQEIEKSGIRIQGKEIAQRNKLIAELKQRASVTSYSEAFQELIEEVAYTWFNRLIAIR
                     FMEVNDYLPDTYRVLSSEISGKIEPDIITDIYDADIFEELSSNEQQQVRTWKTDNSAE
                     AMDKLYQLIFIKICNQLNDYLPELFEEVEDYTELLFTASYIKKDGVIADLLSIPEDDF
                     YVQAGGQVEIIGWLYQYYNSEPHNQVVNIYKGIVKKQDIPAATQLFTTDWVVRYMVDN
                     SLGKYWLDHYPNSELRNKLKYLIPDKYAFEQATSVSNLKPEELKIIDNAMGSGHILVY
                     AFDVLMEIYLTLGYSERDAARAIVEYNLYGLEIDKRAYQLSYFAIMMKGRQYDRLFLK
                     KSLSPNLREFIDSPKVTDEYQERISELNNDKYSERWNEYKELIFDQFENGKELGSIIK
                     LVDKNNQAITKEYLGKVREYISKFNEFTNMDILYGMKEIQEKYLHILDVAEMLVDKYV
                     AVVTNPPYLNKMDKKLKKYVNDNYSDVKKDLFSVFIKLNSQMLIDNGYASFMTPFVWM
                     FISSYEKLRSFLINERNISSLIQMEYSAFEEATVPICTFTIKNSNEQDGSYIKLSDFK
                     GGMEVQKIKTLEAIHQHGECDYFYSTNQDNFKKIPGMPIAYWASDNVITDFAQGKLTN
                     DVADAKQGLATGNNNKFLRYWFEVEFNKIKFDAKTLEDAKKSRKKWFPTTKGGRFRKW
                     YGNNEYVVNWENDGDEIKNYVNENGKLKSRPQNLNYYFKKGITWSTLSSGQVSFRLQN
                     QAIFETKGSVLFSKDITDFEEIFGYVNSKLVNHFLTFISPTLDYHEGPMGKLPFLKVT
                     SERKLKKIREKVDENIDIARKDYNALETSYEFRRNILLSYMFKKDLSEAFNCLSRQLV
                     NQFNTLKSNEEELNDIFINHYSLEKELTKEVQDEYISARLPDKAREIKDFISYSIGCI
                     FGRYSLDEDGLIFAGGEWDPSRYQKFKPDTDNVLLITDDEYFKDSQIDIVNRFIDFVE
                     ITFGKETLEENLQFIADTLGGKGTSREIIRRYFVQDFYKDHLKTYSNRPIYWQLDSGK
                     QNGFKALMYLHRYTPDQLGKVRTEYLHELQKAYDNRIELRQQQISTSDNPKERNAMTK
                     EIVKIQKQLKEVREYDEKLGHLALKRIELDLDDGVIVNYDKLQRDPKTGEKFQIFAKG
                     VKEPKK"
     gene            complement(13665..17336)
                     /gene="brxC"
                     /locus_tag="ACG912_001810"
     CDS             complement(13665..17336)
                     /gene="brxC"
                     /locus_tag="ACG912_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFQ0513122.1"
                     /translation="MLIKDIFAKPIDRNIQGVIKVGQAKDENVQQELEEYVVTKELQK
                     HFKTIFDAYQRSINTPTDKMGVWIQGFFGSGKSHFLKIISYLLANREINDKKAIDYFK
                     EDGKIKDPLTLATMERCSQINTDVILFNIDSKSDAGGKKDKNGIVNVFLKVFNEMQGL
                     STIPHLADFERRLIKEEKFDAFKAAFEEINGAKWQEERHEFDWIQDDIKEAALKIDFM
                     SEAAIDNFLEKASGNYLISIDDFANLVKSYLDSKEADHHIAFLVDEVGQYIGGNRELT
                     LQLQTVVEDLGRICEGKAWVLVTSQEAIDHVTNFDNNSDNDFSKIKDRFATTVSLSSA
                     NADEVIKERILKKKEFANDSLALQFETVQTELKNKLLFVDSPELKLFQDAENYAEVYP
                     FIPYQFNLLGQILTEIRNHSYQGSNLSSGERSLLAMFKEAAERNKENDTTSLVSLDQF
                     YYSLERWIDTAISRVITQATNNSHIQRPTDIDNFNVNVLRILFMIKYVDQAIKPNVEN
                     ITSLMIRNINEDRLETKKRVEEALDLLIKENFVRKNLDSYIFQTDEEQEITREIKNQN
                     ITDSDLIVELSKRIFDGVLDRNSFAYEKLGMSGGNKFKNRYTFTYAQVLDQRPYKQAG
                     NAEMTVNFISPYSDLAGQEAELALKSSRNLLFVELPSDKEFKDDIAMYARMNLYLTTT
                     YSSVVKNFDAIRSEKSGELQELSASINDQLNELLKSAKFFYNGAEIQLTGSDFKAKFQ
                     SALQKMAEEVYHKLNQIDAPKEKEDILYLVKESKNEIIEDPSNEQAVQEIFNFINDQK
                     RNHLTITMKTIRDRFETKQPYGFTELDVEWCIAKLFMSGKIDLLYNNVQISRMDDPNS
                     IVMIIQKRRESEKIKIEPSAVISPKAKKLVDEIGRELFQSRNIVDENNNEHTMENFKK
                     YLKGQLDNLRSYFDLQNTYTYPSAYEYEQIKKYLEVIVQTSDVEDFFYTIKKYRDELF
                     DWAETYDKIKNFHKQNSTQKKIWENAQDKIRLIEKSRRQTSSQEIDQLIHEMKTILKK
                     ADPYSDMMKLNELIQEFIEQYNQLLELESQEILNKLTEEKQATFKLLEGKDFKDQFVP
                     IVDQEFQELKAKIEQANTIDDLVFLSLNVAEQYLIFNQRFDNEENRRRQEEQARKQIE
                     AANLIRETEKDEIDSAAKAEEIAIVKPTIQQKTVPMRTLNVHVKTIKSAADIDELTAE
                     INQKLKEQLQENTEITIQF"
     gene            complement(17361..17936)
                     /locus_tag="ACG912_001811"
     CDS             complement(17361..17936)
                     /locus_tag="ACG912_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFQ0513123.1"
                     /translation="MSLSIQEHLEKLNEVIQKEDFLLNRGLGNEVGYYIFDYDPKDEL
                     IVRDYINDISGTSVGDNYEVKVYNLYVIMLEYLEKRGFTEKAIKMEERRGLQKLVESI
                     GRLLKMDLPEEENVFFEYVDAHTPEKSVVFLTGIGELYPLIRAHSILNKMHQVFDRVP
                     VVMMYPGEYDELSLTILNTNKDDNYYRAFRI"
     gene            complement(17936..18535)
                     /locus_tag="ACG912_001812"
     CDS             complement(17936..18535)
                     /locus_tag="ACG912_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFQ0513124.1"
                     /translation="MNKKYSAGLVSKRFWFSEFKLYIQLRLEGYTDIEIKEKNEHENI
                     FFAVSSNRQKEIYQNVKRRVSQLDEAGLQLFNQLNIDNQKLVNLISILMIDDLFNEFM
                     TEVYAENLRKGNLQLTPVDYRSFFTEKRRSSEDISKWKDYTIKRLIAAYQTNLIETGL
                     VRGEKEYGRMTPILLDKRLIQWLVDKNRKDIVLALGGIL"
BASE COUNT         5742 a         3562 c         2610 g         6726 t
ORIGIN      
        1 agttcatatt ttttatttat accgcttatt tactacttgc tgtactagaa tctgaagtga
       61 atgtactcaa aacattttca aagtctttgt ctttgatttt tacattagcg tctttcaata
      121 cttcgccaat tactttattc tggaaggtac tgtcacttaa ttttgtgtct gttgcaattt
      181 ctttcaattc ttttttgtat ttatccatgt cgttgccttt gttttggttt ttcaccattt
      241 taaccacata atattccgta gtataagttg aagcattggt agaagtgatc acatcagaaa
      301 cttgaccatc tttcaactta aaggctgctt ctttgacttc tgccggaacc gttgtagtag
      361 tggaatcaaa tttcactttt ccgccatctt cttttgtcgt agtatcagtt gacttgtctt
      421 tggctaattt agagaaatcg tctcccttat cagcagcttt cttcacatct tttgcatcat
      481 cttcacttgc tacttggata atttgagctt ccacttctgg atggaaagat tcccaagccg
      541 tttttaaatc ttcatcggtc aatttgatat gtttctttag accttcttga aaggctagac
      601 tttgtttgat actgtctttg aatgtttgtt ctgtgtaacc tgcagatttc aattgtgaat
      661 caaatgaatc acccaattgt ttctttgttt gatcatattg ggaatcgatt tctttggttg
      721 atacgtcatc tccatattta tcttcaaata cttgataaac gatcaactgt tgcacagcgg
      781 aatctgctcc ttgcattttt ttcaagctat ccattacttc ttgttcgctg attgtaccgc
      841 ctttataact aacggctgtt ttttcaccgg cattcgtaca gcctgacaat aagccagtgc
      901 ctagtacgat ggcaagtgct aaaaatcttt ttttcattta tcaattctcc tacttttcgt
      961 tttttgcgaa ttgctaaaaa caaaggtgtt caagatgaaa atcatcaaga acacctttac
     1021 taatctgctc gatacgtcct gaatagatat aaggctacac aatccagaaa gttcgctcag
     1081 cagaataaat gtcaagtagc acatcacacc ttaatatact acttcttttg ttaccgtttt
     1141 tcaagtctat cttgcgttcc gtatttaacg taactaattc ttagaacaaa tgttatcgat
     1201 tttgtttttc ttcttccgct gttgggatat tcaaatcaga cacttttatg ttcgattttc
     1261 cagacatttt tttgttgttt ttattagaaa aagtttgttt tttcatttgt tttctaaagc
     1321 atgatcttct tgtttcctat tttggtcaga tatggatcat gcgtgaccac gataactaac
     1381 gtaccttggg aagcctgttg cgataagatt tcaacgactt gctccttatt tcgtttatct
     1441 aatgatccag ttggttcatc ggctaaaatg attttgctat tcttcaataa gactctcgca
     1501 caggcgattc tttgcttctc accaccagat aatgtgtaga taggcgtact tagcttcttg
     1561 tttatattta cttttttcaa tgcctcatac tttgctgttc gcttatctga aacatttaaa
     1621 ttcgaaaact tcaatccaat atctaaatta aaatcgattg tttcatattc aatcaaccca
     1681 aaatcttgaa aaagaaaaga gagggcattt ctttttatca agcgcttctc tcttccagtt
     1741 aaatgttttg tttcttgatt aaataaataa aattgccctg aactgaattg atcattcaat
     1801 cctaatatat tcaataaagt acttttccct gctccgctct ctccaactac agtcacaaaa
     1861 tccccagcat ccgcagtgaa acgatccaaa tgaagaatcg ttttgtttcg aatgattttt
     1921 ttcgcacctt ttatcactat caacggctcc aattgttaac ctccattcat cagcaataac
     1981 tgacttttct tttctgtaga caaaataaaa acaactgaaa gaataaaatc aatgaagcta
     2041 aagaaaaaaa tattaaaaag atatttaggc tctctttcta taagaaaaag gaagattaca
     2101 cttgtcgttc cccatttgat gaggttcatc atgaagaaat ctttatacgt ttcaaagagc
     2161 gaataaccac ttgaacgaaa gatagcgatt ttccgacaat tgtagacaaa atatatgcat
     2221 aacatgacat agctcaacaa gaagaaagaa aagaaagtga gtaaaaatat ctgtatcaag
     2281 taatgaaatg atctgcttaa cactttgaca gctatctctt cagtctgagt atacggaaat
     2341 gataactgca agttatcacg taactgactt tcttcaatca gctcaaataa cctttctttc
     2401 gacaaagagc ctatcttgat ctttaacggt ggatagattt ctccgctgag aatatttctt
     2461 tcccaatacg tactgttttt caatgtcagt atttcaacaa ccggataatg ttcgatccag
     2521 gggatagaag gaataaatgt ataaatgggt tgattgtttt ttataaatag aaatttcggt
     2581 tctttttcac tgatttcttg ataatattct gttatttgcg ccaggtcatt ttttaaatct
     2641 gtatgtctat atcgttcagg gatcaatata acagggtgcg tttctttttc ggttatttgg
     2701 atcaaccttc cagagacatc aatcaaagga aatttcttaa gataattcag atttattttt
     2761 atccctctgc caaactgttc attttcttca ttcaagtaat ctgatacatt gactaatata
     2821 cttccgtttt tattcaaagt ttcatataat tcctgatcgg ctgctgatgc aaaacgattg
     2881 gaataaataa aatccacgtg gtttttcccg atggttactg gataaaatac atgatagtcc
     2941 tcaccagcga tttcttttgg tgtttcatgg gaaacagcta actgtgaaga agaaaaaacg
     3001 atagttgcta attcgacatt ggcaacggtc aaaagaagag aaaacaacaa tccaaatgta
     3061 aaaagaatgt attggatata gttgaacgct tctcttaacg aagcgatttt ttcaactaat
     3121 aaaagagaaa tcaaagagat accctatatt ccgctgaaca agaaaacgta catctggaac
     3181 aaataaagaa attgctcctt ataatacata aaaataaaac caccaatgat tcctgcaatt
     3241 atgaatgtaa taatgatttt ctttttcccc aataaacacc acaatacttg atgtaatgaa
     3301 aagccattat tcaacaataa actgatttct ttattcatca ataagaaaaa ggatgataac
     3361 aaaaggaata aaagaatcaa tccaatagtt aaatacactc gataattata tacgtctgta
     3421 cttgaaataa ccgaatacga atagctattt tcaaagtctt caaaaatatt agtggcataa
     3481 tcagtgtgaa atgcttgatt gaatcgttcc acaaatagta aaatgaaccg ttcataggct
     3541 tctttttctc tcgtcatcaa gaaaaattca ccttcaaaat cttttatttt gatcgattct
     3601 tcaatggacg ttacgcgaaa ggaggtatta gaatagggta ccttctcgtg agaagatttg
     3661 acatcccctt gataatacaa tgtcatttca agctttggct gtatcttgaa gttccaagat
     3721 gtatctactt cattggattc atttactgtt tttttcatat aagaaagctc agtttctgat
     3781 gagacatttt caaataccat gattagctgc tcatattctt cttgcccaat aaaatgttgt
     3841 ggcaatcgaa tcggagtata agtatctgtt tgatggaaaa attcatttgt ccgatagagt
     3901 acctgtttat ttataataac caaggcaaaa ccactcatga taccggtcac tatgattgtg
     3961 agtaaaactg tgaacttttt tttgattatc accactctcc cttacttaaa tgattctttg
     4021 ataaaatcat accgtttttc atcaaaaaaa cgtatctatc aaatgtatag gtcatgtaga
     4081 ataaatctat atactgtaga atcgaaagga gaagaagatg aacatcggag aaaaaatacg
     4141 aacttaccgc agaaattgta agcttacgca aaaagaatta ggcgaaaaaa tcggtgtttc
     4201 cgataaaaca atctctagtt gggaaaattc tcgtacaatg ccagaccttg aaatgctgtc
     4261 acttttacat cagactttag gtctgccgat tgattttcct tctattgaat cagctaccga
     4321 atcaatagaa ccaacattgc cggatgaacc tcttgttatc aagaaatctt attcatcaaa
     4381 acaacccttc tcactcaaaa agacactatc tttctttctg ctcgtctttt gtttggtcta
     4441 tctttttctt atcaattatc aatataaaaa tgaaacggtt gatcgattca atcctttttt
     4501 gaaaccacaa atcggctata ccacactccc tgtaggatac gtggaaaatt ccatcgaaca
     4561 ttgggtatgt aatgatatct ttgttcatgg tacatggctg tcttttcacg ctggagaggt
     4621 agaaaaaggt aagaatcatg ctgtaatcaa acacaaagga aaacacgtca tgaacgttcg
     4681 cctgctcact tgggaagcca ttccctcaga ttaccaagaa atcctcatcc aagactatga
     4741 cgatgcctta aaaagaagcc ctcaggagcc agataggcat taagtaggag atgaacgtag
     4801 agatagaaaa aatcaatgca catgatctta tcaaaaaaaa acaggggtac aaattaattg
     4861 aaaaagcttt gagatgaaag ttcctaaata aaaaagttca gaatgatagg taaaaaaatg
     4921 actaactatt ctagtattag gctatttgta ttattcttct gttattttta tatcaaaaaa
     4981 tccagaaata actaaatata ggtatttctg gcttatagta taatatttac gccacagccc
     5041 ccaatgattc tttttggaat catgatcaag gacggacata cacaaaaata ttttgcccat
     5101 tcgatccgaa tagattaacg actaatctag taagtaatat agattggtac gtagtcaaac
     5161 cagcaaatgg aaaagttgtc tattccaccc caagggcttt aaagacagca tcttccggcg
     5221 tttgataaaa aatcaaatta aaggtaccca ttaattctgg gggtacagtt ccaatatcag
     5281 cagcgcttat cataggtaca agtacttttt tcgctccggc atcttttgct acttgtaaac
     5341 agttagctaa attatctacc ttaatcatag aaccaccaat tgtaatatcc cctaaaataa
     5401 ctaaacttgg taatacaggt ttatttaagg aaactgagca taaagctatt atagttgata
     5461 acgataatgt ttttgtcata ccaatgccat tcatatcttg aacatgaacg acaaaatctt
     5521 ttgtcgtcaa actaatagac tgactaacac ttccataatt agctcttaaa tatcggtatg
     5581 ccgaatccat cgcttctctt gctccagcat ctgaattgat cccagtacgt tcaaactttc
     5641 catttcccga agtcatttgc gtttccaatt tataggtacc taattgccca gttttgccat
     5701 aagataccgt gtacaaatgc cctggcttcc ctaacccttc tggaatcaaa gttcctccac
     5761 cttgttcagg aacagaaaca tactcttctc gctcttcttc taaatcaatg tatgaaaaat
     5821 tgacatcata aaattccatt ccaccaattt tctttaattg ttcttttaca cgacgacgca
     5881 tttcaagagc aaatcttagt atttcttcga catcactttt ttcaaatcta ccatcaggat
     5941 acaacagttt catcatccca gatacaatcc gacgaactgc aatcgtatcc cgctgattta
     6001 attgagaacc aagtttgaaa tatttatcaa aactatcgcc aaaactaact tttcgaagct
     6061 cacgcatgac ttcagaaaaa taatctgtaa tgaagccata gtcatttgta aaaaaatctg
     6121 gtcgatattt aggaatttcc caacctggta aataacaatg catccgatcc aaaaaggctg
     6181 aatccgtccc cataacctcc ggaaatggct caaataaatt cgaagttttc aatattacat
     6241 caacgctttg attaatattt cctacaaaag ccattgatgc tgaagcattc ttttcttctt
     6301 tgcctctagc aaaagatcct gaagccatgt aatctttcat gatttgaacg ccatcattat
     6361 ccttaaattt aatcccagca acttcgtcaa aagcaacaca gtcccacata cctacaagtc
     6421 ccattgtacg actagccata ttatagaaaa ggtttgctac tgttgtttgt ccacctgaaa
     6481 caaggattga gtttggtgaa atttctttat atacatgcga tttacctgta ccacgtggtc
     6541 ctagttcaca gagattaaaa ttattttcaa ttaatggaat aagtcttgct aaatgaagcc
     6601 attttaccct tttattaaat tgtgtaggtt ccataccaat ggaccgtaga agtaagtcaa
     6661 tccattcatt tgtagtaaac tccttgcgag ccatcttgta agcctcaatg tctaaaccag
     6721 gcatttggat tggatcaacc ttttttgcaa taaaagggct attccaacga tcattctcat
     6781 cataccaata ttccatcgaa acaatacacc agatcccccc acctaataat cgatcaaatt
     6841 ttttcggata ctcttcacta atttgaacat tttttaatcc caggttagag aattctgctt
     6901 cataacaatc tttcttttca tttaaatgta cagttacttt atcaataact gtataaccac
     6961 ccaattcacg aatttttgct tttacttttt ctgcttcatc aggacgcaca tagtttcgag
     7021 caagaatatt tttaacattt tccacacctt tttcaaccaa tgtttcatcc aatgacgaag
     7081 catacatacc aagtaaatat tccaatacat aaacaggtac attcgcacct tctttaattt
     7141 tctttgttaa atcttttctg acaatctttc cgggaaagaa ttcagataac tttaaaattt
     7201 cctgttgttc cactagaatt cctccatgtg ttcagtcatt ataatagcaa ttaaagtata
     7261 tcatacttaa ccagtaggct tgataataca aaagaaaaag tatccacaaa aaattgtgga
     7321 tacctcgttt ttatatatca aaatcaaatg attctgcaaa aggattatct ataatgaatt
     7381 ttattcgctc ggtcaaatca tttttttgat tttttatcac taaataataa gtatcttcaa
     7441 ctgtatatcg acgatttaca aattcaaaaa caaatttagt aaaccgctga tttgcatctt
     7501 ctgtttttga atccgcaaca tagatttctt catttgagat acgttcacca tattcatctt
     7561 caaaataaag ccagaaagat gcggggtcat aaacatcact aattgcattt ttttgataga
     7621 attctaacgt ggttgataat cctgtaattc tgcggttgct agtcattaat tcaattgcta
     7681 ctggttcctt tgcattgcta cctttggcta ccgtaatttc tagaacaggg actagcattt
     7741 cttgaataga tgaccctcca tgaacgtagc tttgtccccc gccagctttc ttaaaaatac
     7801 ttgaagtaat cgggacacaa atgtttctag tgtcatcatt tcctaaagaa tctcccaaag
     7861 agtaattttt cgtaccaatt atttcgtatt cttcttcact aagaataaaa cgacgttcta
     7921 cacgatcttt ttcatttcta gatggatttt caatcttatc tacttcttca acgtggcttc
     7981 ttttataaat aaatccatga tcagccgtaa ctataaaacg atagatactg ccatcattat
     8041 gcaagcgatt aataagtcgg gaaatttcgc ttatcgctaa atcacaggcc tcaaaaactc
     8101 gattttcatt cgagtgatca cctgttctat ccacttgatc gtgataaata taaatgactg
     8161 atttgccatt aaatacttct cgtacctcag ccttattcat cttaaccaca tcatcataat
     8221 ttaaagctac agcatttgca tctcgttgct ggagaatcgc atttcgattg gataaaccat
     8281 tcgttttttt cccatcaatc agtacttctg ttccttcatt gtaagttagt tgattgtttg
     8341 gtaataaggc ggctttaccg aattcagtaa ctgaaggtaa tacagaaaga aagctagcca
     8401 tgtttgttga atacttcttt tctctaacta acttacgttc taaattcatc cctaactcat
     8461 atcgaaaagc atctgaaata ataactacag ttttaacaga gcgtttcatt acagcttgat
     8521 tataaaattt tcgaatggat ggacgattct taaattcaaa agtttgattc caaatttgtc
     8581 ctgattgatc aagaaacttc gaataatttg cttcaactaa tcgatacaat tggccatatt
     8641 tttgcttttc ttctatatga tcactattcc aaataaattt ccgataatgc gtatcaaccc
     8701 aatacatttc cttctcatat tgagttagca aattatccat gttcatcggc aagcacaatc
     8761 tgtttttatc cacaagataa taagcatgtt tcaataattg atactcataa atataatccg
     8821 atgcgaaatg cattttttcg cgctgcttta aactatcaaa aattgtttgt cctgcaattt
     8881 tcgttgtagt gtcagaagcc tttaatcgtt ctgcataaaa cattaatatt tttgcatgaa
     8941 tctcagaaaa tacgtctgct tcaattagat catcaatttc tatactgtct aataatccat
     9001 caccatcaat agcctgatat acttttttag atagctgttc aaagctttcc atgtaacgtg
     9061 tatcattcat aaactgatcc atgaaagtaa taatgtttgc tttcaaatgt agtatttcaa
     9121 atcctttgaa cttcttcggc atctcttgat caatttgata ataaaaatag ttcaaataaa
     9181 gacacatcgt cagcttttcc aatgttggct ggctatccgt aaagccaaat ttcatttgaa
     9241 taaattccca aaatttagct gttaactgat acttatcaag ttcccttaaa ggattctccc
     9301 tactttctgt aaagcttttt agaactacac gcagtaaatc atttaatcga atacttttcg
     9361 atttcaacaa aacagccatt atcattaatt caatcgtttc tgatttatta attgtcatat
     9421 gcgtactaaa ttcaccaaaa cttctcatgc gatcctttgc attaaagaaa ttacgatact
     9481 gtttaactgt ctgatgaaat ctaagtggaa tagccaattc attcatgata atcgctaagc
     9541 ggtctgcact aaattcctct gaataattca atacactcaa caaatgatta tcttcatctt
     9601 tatcatccca acgaggaaat ggcgcataga ttaaatagtt agtttcggga tcttcatatt
     9661 ccaataaacg cttagtttta aattgtgcgt ccttttctaa tcgatagatt tttgcatttt
     9721 gaatgaccaa tgagtcaatt tcttcaagaa aatcctgatt tgaatcaaac caaaaaatga
     9781 ttttcctaca tccataatta ggaaaacgtt gatttaattt ttcttcaatt tgttgcgtat
     9841 ttatatctgt catcaatctc acctaatcgt tctctttcta taccccttat attgacataa
     9901 tttggataat ttcgcacgca atttaagaat aaaaaagaaa gttgttagaa aacaactttc
     9961 ttagataact atttttttgg ttctttgacc ccttttgcaa aaatttggaa tttctcgccg
    10021 gttttaggat cacgctgcaa cttatcataa ttgacaatta caccatcatc taagtctaat
    10081 tcaattcgtt ttaatgctaa gtgacccaac ttctcgtcat actcacgaac ttcttttaat
    10141 tgcttctgaa tctttacaat ttcttttgtc attgcatttc tttcttttgg gttatcactt
    10201 gttgaaattt gttgctgacg caattcgatt cgattgtcat acgctttttg tagttcgtgt
    10261 aggtattcag tacgtacttt tcctaattga tctggtgtat agcgatgcaa gtacattaac
    10321 gctttaaagc cattttgttt tcctgagtcc aattgccaat aaattggtcg attggagtat
    10381 gtttttaaat ggtctttgta aaaatcttgt acgaagtaac gacgaataat ttctcgactt
    10441 gtccctttgc cacctaaagt gtcagcgata aactggagat tttcttcaag ggtttcttta
    10501 ccaaaagtaa tctcaacgaa atcaatgaaa cgattgacaa tgtcaatttg gctatccttg
    10561 aaatactcat catcggtgat gagtaaaaca ttatcagtat caggtttaaa tttttgatag
    10621 cgggaaggat cccattcgcc gccagcgaag atgagaccat cttcgtctag ggagtaacga
    10681 ccgaagatac aacctattga ataagaaata aaatccttaa tttccctcgc cttatcaggt
    10741 aaccttgcag agatatattc atcttgaacc tcttttgtta attctttttc aagagaataa
    10801 tgattaatga atatgtcgtt cagttcctct tcattcgatt ttagagtatt aaattgatta
    10861 accaactgcc tacttagaca gttaaatgcc tctgagagat cttttttaaa catataactt
    10921 aacaaaatgt ttcttctaaa ctcgtatgac gtttcaagag cattatagtc ttttctagct
    10981 atatcgatat tttcatccac tttttctctt atttttttta attttctctc agatgtaact
    11041 ttaagaaaag gtagttttcc cattggtcct tcatggtaat ccaaggtggg agaaataaaa
    11101 gttaaaaagt gattaactaa tttagagttt acatagccaa atatttcttc aaagtcagtt
    11161 atatcctttg agaacagaac agatccttta gtttcaaaaa ttgcctgatt ctgcaatcta
    11221 aaagatactt gtccagatga aagtgtcgac caagtaattc cctttttaaa atagtaatta
    11281 agattttgtg gtctagattt taacttccca ttttcattaa cataattttt tatttcatct
    11341 ccatcattct cccagttaac cacgtattca ttgtttccat accattttct aaaacgacca
    11401 cctttagttg taggaaacca tttttttcta gactttttag cgtcctctaa tgttttagca
    11461 tcaaacttta ttttgttaaa ttctacttca aaccaatacc gcaaaaattt attgttattt
    11521 ccggttgcca acccttgttt tgcatctgca acatcgttag ttaattttcc ttgagcaaaa
    11581 tcagtgatta cgttatcgct tgcccagtaa gcaatcggca ttcctgggat ttttttgaag
    11641 ttgtcttgat tagtactata aaaataatcg cattcaccat gctgatgaat tgcttctaac
    11701 gttttaattt tttgaacttc cattccacct ttaaaatctg ataattttat atagcttcca
    11761 tcttgttcat ttgaattttt tatcgtaaac gtacaaattg gaacggttgc ttcttcaaaa
    11821 gctgaatact ccatttgaat caaactagaa atgtttcttt cattaattaa aaatgagcgt
    11881 aacttttcat agctagaaat gaacatccaa acaaatggcg tcataaagga agcatatcca
    11941 ttatcaatca gcatttggct attcaattta ataaacacag aaaatagatc ttttttgaca
    12001 tcagaatagt tatcatttac atattttttt aatttcttat ccattttatt aagataaggc
    12061 ggattagtga ctaccgctac atatttatca actagcattt cagccacgtc aagaatgtgc
    12121 aagtattttt cttgtatttc cttcatccca taaagaatat ccatattcgt aaattcgttg
    12181 aattttgata tatactctct tacttttcct aagtattctt tagttattgc ctgattattt
    12241 ttatcgacta acttaatgat tgatccaagt tctttcccat tttcaaattg atcaaaaatt
    12301 aactctttgt attcattcca gcgttcacta tacttatcat tgttcaattc actaatacgc
    12361 tcttgatact cgtctgtcac tttaggagaa tcaataaact ctcttaaatt cggcgataga
    12421 ctttttttca aaaaaagacg atcgtattga cgccctttca tcataattgc aaaataagac
    12481 aattgatacg ctcttttatc gatttcaaga ccatataaat tgtattcaac gattgcacgt
    12541 gcagcatcac gctcagaata tcctaacgta agataaattt ccatcaatac atcgaaggcg
    12601 taaactaaaa tatgcccaga ccccattgcg ttatcgataa tttttagttc ttctggtttt
    12661 aaattactta cagatgttgc ttgttcaaaa gcatatttat ctggaattaa gtatttcaac
    12721 ttatttctta attcactatt gggataatga tctaaccaat actttcctaa actattgtca
    12781 accatataac gaacgaccca atctgtggta aatagttgag ttgctgccgg aatatcttgt
    12841 tttttgacaa tccctttata aatattcacc acttgattat gtggctcact attatagtac
    12901 tgatacaacc acccaataat ctctacctgt ccaccagctt gaacatagaa atcatcctct
    12961 ggaatactta aaagatcagc gattacacca tcttttttaa tataagaagc tgtaaacaaa
    13021 agttctgtat aatcttctac ttcttcaaat agctcaggta agtaatcatt caattgatta
    13081 cagattttta taaaaatcaa ttgatacagc ttgtccattg cttctgctga attatctgtt
    13141 ttccaagttc tgacttgttg ctgttcatta ctagataatt cttcaaagat atcggcatca
    13201 taaatatctg taataatatc tggctcaatt ttcccactta tttcactaga taatacacga
    13261 tacgtgtcag gtaaataatc atttacctcc ataaaccgaa tggcaatcaa tcggttaaac
    13321 cacgtataag caacctcttc gattaactct tgaaaagctt ctgaataaga agtgacagaa
    13381 gcacgttgtt ttagttctgc aatcaatttg tttctttgcg ctatttcttt tccttgaatt
    13441 cttattcctg atttttcgat ttcttgtaaa ttgctatcaa ttttcttcac atcttcaatt
    13501 tttgattcat caattcctaa acgttttgct ttagtcatta cttcatcaat taattttcta
    13561 cgggcattga ccgcgaaatt ctttatcgct ttcttatcca tttttctttc ccttctatat
    13621 gtaacctttg aaaagacagg caccttttta tgcctgtctt ttcactagaa ttgaatcgtg
    13681 atttctgtat tttcttgaag ttgttccttc agtttttgat taatttcagc tgttaactca
    13741 tcaatatctg ctgctgactt aatagtttta acatgaacgt taagtgttct cattggtact
    13801 gttttctgtt gaatagtagg ttttactatc gctatttctt cagcttttgc tgcagagtct
    13861 atttcatctt tttcagtttc acgaatcaag tttgctgctt caatttgttt acgtgcttgt
    13921 tcttcttgac gtctgcggtt ttcttcatta tcgaaacgtt gattaaagat caaatactgt
    13981 tctgctacgt ttaatgataa gaaaacaaga tcatctatag tattcgcttg ttcaattttt
    14041 gctttcagtt cttgaaattc ttggtcaact attggtacaa attgatcctt aaaatctttt
    14101 ccttctaata atttaaatgt tgcttgtttt tcttccgtta atttatttaa tatttcctga
    14161 ctttccaact ctaacagttg attatattgt tcaataaatt cttgaatcaa ctcatttagt
    14221 ttcatcatat ctgaataagg atcagccttt ttcaaaatgg ttttcatctc atgaatcaat
    14281 tgatcaattt cttggctaga agtttgacgc cgactttttt cgattaatct aattttatct
    14341 tgtgcattct cccagatttt tttctgagta ctattttgct tgtggaaatt tttaatttta
    14401 tcatatgttt cagcccagtc gaataattca tcgcgatatt ttttgattgt ataaaaaaag
    14461 tcttcaacat ctgacgtttg aacaattact tcaagatatt tttttatttg ctcatattca
    14521 taagcactag ggtaggtata agtgttttgt aaatcaaaat aagagcgtaa attgtctaat
    14581 tgacctttta aatatttctt gaaattttcc attgtatgtt cattgttatt ttcatcaaca
    14641 atattacgac tttgaaacag ttctctaccg atttcgtcaa ccaatttttt tgcttttggc
    14701 gaaatcaccg cgcttggttc aatttttatc ttttcacttt ctcgtcgttt ttgaataatc
    14761 attacaatac tatttggatc atccatccgt gaaatttgta cattgttata aagtaaatca
    14821 attttaccag acatgaaaag tttagcgatg caccattcca catcaagttc tgtgaatcca
    14881 tacggttgtt ttgtttcaaa acgatcacga attgttttca tcgtaatcgt taggtgattt
    14941 ctcttttgat cattgataaa attgaaaatt tcctgaactg cttgctcatt tgatggatct
    15001 tcaataattt catttttgct ttctttcact aaatataaga tatcttcttt ttctttaggc
    15061 gcatcaattt ggtttaattt atggtatact tcttcagcca ttttctgaag tgctgattgg
    15121 aattttgctt taaagtcgga gcctgtcagt tgaatttctg caccgttata gaaaaatttt
    15181 gcagatttca acagttcatt taattgatcg ttgatacttg cacttagttc ctgtaattca
    15241 ccagattttt ccgaacgaat cgcgtcaaag ttctttacta cactcgaata agtcgttgtt
    15301 aaataaagat tcattctagc atacattgct atatcatctt taaattcttt atctgatggc
    15361 aattctacga ataataagtt acgggaactc ttaagcgcaa gttctgcctc ttgacctgct
    15421 aaatcagagt atggtgaaat aaaattaact gtcatttcag catttccagc ttgcttgtat
    15481 ggacgttgat ctaacacttg tgcataagta aacgtatacc ggtttttaaa tttatttcca
    15541 cctgacatgc ctaatttttc ataggcaaaa ctatttcggt ctagaacgcc gtcaaaaatt
    15601 cgtttagata gttctacgat caaatcacta tctgtaatat tttgattttt aatctcacga
    15661 gtaatttcct gttcttcatc agtttggaaa atataactat ctaagttctt ccgcacaaag
    15721 ttttccttaa ttaaaaggtc caatgcttct tctactcgtt ttttagtttc caggcggtct
    15781 tcgttgatat ttcttatcat caagctcgta atattttcaa catttggttt gatggcttga
    15841 tcaacatatt taatcatgaa taaaattcgt aagacattta cgttaaagtt atctatatca
    15901 gttggtcttt gaatatgaga attatttgta gcttgtgtaa tcactcggct aattgctgta
    15961 tcaatccaac gttctaacga gtaataaaac tgatctaaag aaaccaaact ggttgtatca
    16021 ttttccttat ttcgttcagc ggcttcttta aacattgcta gtaatgaacg ttcaccagac
    16081 gataagtttg atccttgata gctgtgatta cgaatctcgg ttagaatttg ccccaataaa
    16141 ttgaattgat agggaataaa tggataaact tctgcgtaat tttcagcatc ttggaagagc
    16201 tttaattcag gactatcaac aaagagtaat ttatttttca attctgtttg aacagtttca
    16261 aattgtaagg ctaaagaatc attggcaaac tcttttttct tcaaaatacg ttctttaatt
    16321 acttcatcgg catttgctga agataaactc acagttgtgg caaaacgatc cttgatctta
    16381 gaaaaatcat tatcagaatt gttatcaaag tttgttacgt gatcaatcgc ttcctgactt
    16441 gtcactaata cccaagcctt accttcacaa attcgaccca aatcttccac aacggtttgt
    16501 aactgcaaag tcaactcacg attaccgccg atatattgtc cgacctcatc gactagaaag
    16561 gcaatatgat ggtctgcttc tttggaatca agatagcttt taactagatt tgcaaagtca
    16621 tcaatgctta ttaaataatt tcctgatgct ttttctaaaa aattatcaat agcggcttca
    16681 ctcataaaat cgattttaag tgccgcttct ttgatatcat cttggatcca atcaaactca
    16741 tggcgctctt cttgccattt tgccccatta atctcttcaa aagcagcttt gaatgcatca
    16801 aacttttctt ctttaattaa ccgacgttca aaatctgcta gatgtggaat cgtgcttaat
    16861 ccttgcattt cgttaaatac ctttaaaaaa acattcacga ttccgttttt gtctttttta
    16921 ccacctgcat cactttttga atcaatatta aacagaataa catctgtatt tatttgtgag
    16981 caccgttcca tcgttgccaa agtcagtgga tctttgattt tcccatcttc tttgaagtaa
    17041 tcaatggctt tcttgtcatt tatttctcga tttgcgagca agtaagaaat aatttttaag
    17101 aaatgagatt tacctgaacc aaagaacccc tgaatccaaa cacccatttt atctgttggt
    17161 gtattgatac tccgttggta ggcatcaaaa attgttttaa agtgtttttg tagttctttc
    17221 gtaaccacgt attcttctaa ttcttgttga acattttcat cctttgcttg accgacctta
    17281 ataactcctt gaatatttcg gtcaattggt tttgcaaaaa tatctttaat tagcatttaa
    17341 tctcctacct tccatcccaa ctaaattcta aatgctcgat aataattatc atctttattg
    17401 gtattaagaa ttgtcaaact taattcatca tattcccctg gatacatcat cacaactggt
    17461 acgcgatcaa atacctgatg cattttattt aaaatagagt gcgctctaat taatggatat
    17521 aattcgccaa ttcctgtcag gaacacaact gatttttctg gtgtatgggc atccacatat
    17581 tcaaaaaata cattttcttc ctcaggtaaa tccattttca ataaacgacc aatgctttcc
    17641 actaattttt gtaaaccacg tcgttcttcc atcttaatcg ctttttcagt gaaaccgcgt
    17701 ttttctagat actctaacat aatcacatag agattataaa ctttcacttc ataattatca
    17761 ccaacagatg ttccagatat atcattaata taatctctta caattaactc gtctttcgga
    17821 tcgtagtcaa agatataata gcctacttca ttacctaaac cacgattcaa taaaaaatct
    17881 tctttttgaa ttacttcatt caacttttcc aaatgctctt gaatcgataa tgacattata
    17941 aaattcctcc cagtgctagt acaatatctt tacgattctt gtcaactaac cattgaatca
    18001 atcgtttatc taataatatc ggagtcattc gtccatattc tttttcccct ctgactaatc
    18061 ctgtttcaat tagattcgtt tgataagcgg caataagccg tttaatcgta taatctttcc
    18121 attttgaaat atcttctgaa cttctccgct tctctgtaaa aaaagaacgg taatcaactg
    18181 gtgttaattg caaatttcct ttacgtagat tttcagcata tacctcagtc ataaattcat
    18241 taaaaagatc atcaatcata aggatggaaa tcaaattcac taacttttga ttatctatat
    18301 ttaactgatt gaaaagttgc aatccagctt catccagttg actaactctc cttttcacgt
    18361 tctgatagat ttctttttga cggttactgc tcactgcaaa aaaaatattt tcatgttcat
    18421 ttttctcttt aatttctata tctgtatatc cttctagtcg taattgaata tataacttga
    18481 actctgaaaa ccaaaagcgt ttagaaacta gaccagcaga atatttttta ttcaatagat
    18541 tgcctcctaa tcatctataa ttcaataagt aaacaagtgt tttttgctca tcgttagcat
    18601 tcatttttta gtatctacta tacaatacat atttattata
//
LOCUS       DAZKOW010000057        18504 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000057 DAZKOW010000000
VERSION     DAZKOW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.057"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            99..746
                     /locus_tag="ACG912_001813"
     CDS             99..746
                     /locus_tag="ACG912_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFQ0513125.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="ACG912_001814"
     CDS             1139..3784
                     /locus_tag="ACG912_001814"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFQ0513126.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="ACG912_001815"
     CDS             4094..5407
                     /locus_tag="ACG912_001815"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFQ0513127.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="ACG912_001816"
     CDS             5397..6050
                     /locus_tag="ACG912_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0513128.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /gene="radC"
                     /locus_tag="ACG912_001817"
     CDS             6105..6797
                     /gene="radC"
                     /locus_tag="ACG912_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFQ0513129.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="ACG912_001818"
     CDS             6947..7147
                     /locus_tag="ACG912_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0513130.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="ACG912_001819"
     CDS             complement(7401..9080)
                     /locus_tag="ACG912_001819"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0513131.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="ACG912_001820"
     CDS             complement(9082..9294)
                     /locus_tag="ACG912_001820"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFQ0513132.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="ACG912_001821"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="ACG912_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFQ0513133.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="ACG912_001822"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="ACG912_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFQ0513134.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="ACG912_001823"
     CDS             13269..13466
                     /locus_tag="ACG912_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513135.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="ACG912_001824"
     CDS             13619..14104
                     /locus_tag="ACG912_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFQ0513136.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG912_001825"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="ACG912_001825"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFQ0513137.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="ACG912_001826"
     CDS             14981..15508
                     /locus_tag="ACG912_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513138.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="ACG912_001827"
     CDS             15599..16336
                     /locus_tag="ACG912_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFQ0513139.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="ACG912_001828"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="ACG912_001828"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFQ0513140.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="ACG912_001829"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="ACG912_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFQ0513141.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5984 a         3199 c         4022 g         5299 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acaaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgt
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DAZKOW010000058        18095 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000058 DAZKOW010000000
VERSION     DAZKOW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18095
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.058"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..1891
                     /locus_tag="ACG912_001830"
     CDS             191..1891
                     /locus_tag="ACG912_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFQ0513142.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            1861..2079
                     /locus_tag="ACG912_001831"
     CDS             1861..2079
                     /locus_tag="ACG912_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFQ0513143.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            2099..2989
                     /locus_tag="ACG912_001832"
     CDS             2099..2989
                     /locus_tag="ACG912_001832"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFQ0513144.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            3007..3291
                     /locus_tag="ACG912_001833"
     CDS             3007..3291
                     /locus_tag="ACG912_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513145.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            3778..4392
                     /gene="gmk"
                     /locus_tag="ACG912_001834"
     CDS             3778..4392
                     /gene="gmk"
                     /locus_tag="ACG912_001834"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFQ0513146.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            4397..4702
                     /gene="rpoZ"
                     /locus_tag="ACG912_001835"
     CDS             4397..4702
                     /gene="rpoZ"
                     /locus_tag="ACG912_001835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0513147.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            4955..7363
                     /gene="priA"
                     /locus_tag="ACG912_001836"
     CDS             4955..7363
                     /gene="priA"
                     /locus_tag="ACG912_001836"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFQ0513148.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            7378..7869
                     /gene="def"
                     /locus_tag="ACG912_001837"
     CDS             7378..7869
                     /gene="def"
                     /locus_tag="ACG912_001837"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFQ0513149.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            7862..8800
                     /gene="fmt"
                     /locus_tag="ACG912_001838"
     CDS             7862..8800
                     /gene="fmt"
                     /locus_tag="ACG912_001838"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFQ0513150.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            8800..10158
                     /gene="rsmB"
                     /locus_tag="ACG912_001839"
     CDS             8800..10158
                     /gene="rsmB"
                     /locus_tag="ACG912_001839"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFQ0513151.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            10171..10911
                     /locus_tag="ACG912_001840"
     CDS             10171..10911
                     /locus_tag="ACG912_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFQ0513152.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            10908..12977
                     /gene="pknB"
                     /locus_tag="ACG912_001841"
     CDS             10908..12977
                     /gene="pknB"
                     /locus_tag="ACG912_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFQ0513153.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            13142..14041
                     /gene="rsgA"
                     /locus_tag="ACG912_001842"
     CDS             13142..14041
                     /gene="rsgA"
                     /locus_tag="ACG912_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFQ0513154.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            14054..14704
                     /gene="rpe"
                     /locus_tag="ACG912_001843"
     CDS             14054..14704
                     /gene="rpe"
                     /locus_tag="ACG912_001843"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFQ0513155.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            14705..15343
                     /locus_tag="ACG912_001844"
     CDS             14705..15343
                     /locus_tag="ACG912_001844"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFQ0513156.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            15498..16352
                     /gene="mreC"
                     /locus_tag="ACG912_001845"
     CDS             15498..16352
                     /gene="mreC"
                     /locus_tag="ACG912_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFQ0513157.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            16356..16865
                     /gene="mreD"
                     /locus_tag="ACG912_001846"
     CDS             16356..16865
                     /gene="mreD"
                     /locus_tag="ACG912_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFQ0513158.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            17120..>18095
                     /locus_tag="ACG912_001847"
     CDS             17120..>18095
                     /locus_tag="ACG912_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFQ0513159.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTT"
BASE COUNT         6142 a         3278 c         3907 g         4768 t
ORIGIN      
        1 ttaaaattcc attacaaaat cacgttttca ttgaaaacac tctcattttc aattaatttt
       61 tgtgattttt gtattttttt atttaaagga ggttttatga attgttttct tatggaatct
      121 acaaagtcga atacgagact catgaccttt ttatgacgta ctggtatact tgaagaaaaa
      181 ggagagcgcg atggagataa aaaaagttta cttttcacag aaaatgtcgc tgtattcgcc
      241 tatccagcaa gcaaaacaac cccttccatt ttttcaaagt catcaagaaa gtagagacgc
      301 tttttggcgt tctgcaaaaa agcaatgtgc ttttaaagca gtatcgaaaa aatataccgt
      361 ctattttact attgctaaaa gtagaggaga tgttattttt aataatctgt tgattgaagg
      421 tgaaactttt gaatggaaaa aattttttcg gtatatggaa gcttgcgctc gtttttttat
      481 taaagaaaac tgttgttttt tatttcaatc tcctctatca gaagaatggt tgcgtatttt
      541 tcataaaaac ggctatcaag gaaccacaca attaaataaa aagctggtct atcatacagc
      601 acttgtttta ggtggaggag gagcgcacgg tgcttatcag attggtgtgt ggcaggcgct
      661 gaaggaacat gatatcaact ttgagattat cacaggtacc tcggtaggtg cgttgaacgg
      721 cgcattgatt ttacaaggag atatgaaaaa agctgtgaat ttatggaaaa agctgtcgac
      781 aagacaagtg cttgcgttac cagaaatggc tgcagttgaa gaccttcgtg aacgcttcta
      841 tcgagaaaga cgccaaatga cgaaaacagc actaattgaa ggcggggttt cttctgctcc
      901 attggaaaag atggtcaaag acaatttgga tttgtcctta ctcaaacata atcccaaaat
      961 acgtttatat acggtgtcta ctaaactgcc agaactagcg gaagtagtga ctgatatcca
     1021 acagacaaaa acagaagaaa taccagattg gatactcgcc tctgcttcat tttatcccgc
     1081 tatggcttat cgaaagatcg gaaaaaataa atatgcagac ggtgggtatc gcaacaaaat
     1141 acctattgat attgcgatta acaaaggagc aacagaggca tttgtcgtag atgtgcaagg
     1201 gccaggccca gcgaaaaaaa ttcgtatgcc agacattttt atccattgga aatgtcaaac
     1261 attgtggaca ctaggcagct ttctgctttt tgatagccaa aggaaccaat taaatttaca
     1321 attaggctat ctggaaatga aaaaacgatt aggctattac tatgggaact ggtatacatt
     1381 tcattctgtt aaacctgcaa gaacatactg gcgaggattt ttgtcttact taacaaaaga
     1441 aattcagtta gaattatctt ttttcaaaag cataaaattt tggcaaaaac tgcgtaattt
     1501 atataagaac agggtcgtac cagagacatg cggattggcg atgctagaac ttttagcaaa
     1561 aaaacatttt ttactgccca atgaaatcta tcaagtagac atgatgattc aaatgatctg
     1621 ccaacaagat ataaaaagta tgccggatga tttattggca caaattggtc aattaagtac
     1681 tgaagaatgg cgaaggtatc gaaaatacca gcaaaaaata caaaatgagc gcacgaaaac
     1741 tgtttatttt gattatctgc tacaaggaaa acgcaaggat cgtttgcagc atgaattgct
     1801 taagcaacca gttgacacat tactgatcct atatttatat tatctcaagg aggaacaacc
     1861 atggcacaag aattttcata tgaaatcgta gaagaaatcg ctatattgtc agaaaacaac
     1921 aaggggtggc gtaaagaact gaacctagtc agctggaatg gtcgcccgcc aaaattcgat
     1981 cttcgagatt gggcgccaga ccatgaaaaa atggggaaag gcctgacatt gacgaacgaa
     2041 gagtttgaac aactgcaaaa agcaatagaa aatatgtaat gaggaaggac cctgtcgaat
     2101 gaaaagtatg actggttttg gcaaagccat ccgcgaaaca aaagcgtatc agttagatat
     2161 tgaaataaaa agtgtcaacc agcgttttct agatgttcaa atacgaagtc ctaaaatcct
     2221 gaactttgtt gaaaacgatt ttcgtcagct tattaaacag cagttaagcc gcggtcgaat
     2281 cgaagtattt gtgaatctag cttatattgg caaaaacgaa aaacagatcg tcatcaactg
     2341 ggaattgatt gattcactta tcactcaatt gacagacgga attcaacaac gatacggtag
     2401 tcaaaatcaa gtgtcaatta gtcggatggc ggaacgcttc gtgctggacg aaaacttcgt
     2461 aacggtagaa gaaaaaccgg aagagagcat ggaagaattg gcacagttag ctctagatac
     2521 attgaaaagc gcacttgatt ctattgaggc gagtcgagaa aaagaaggtc aagctttagc
     2581 tttagtgttg aaaaagaata ctgaagaatt caaagaagta ctaaaaaaac tcgatagttt
     2641 tgtagaaatc tatgaaaagg aatttcaaat aaggtatcaa aaaaagctgg aggaatttct
     2701 tggagcgaaa gtcgatgagc aacggttgtt gacggagcta gccatcttgt tggaacgcgg
     2761 agatattcac gaggaactgg atcgtctggc gatccatgta caaaaactag atgaacttat
     2821 cgaagcacaa tgtcctgtag gtcgggaatt agatttcctg atccaagaaa tgaatcggga
     2881 aatcaacacg atcggttcca aatcaaatgc aatcgaaatt aaaaatcaag tcgtccagtc
     2941 taagacgatc ttagaaaaaa tcagagaaca agtacaaaat attgaatgac gaaagaggta
     3001 gagataatga aatattatga cgtaacgttc cacgaattaa gtggaaaaac tgtggttaaa
     3061 cgggatatcc cttctgaaaa aaacggcttt gacgtatgga aagatgcctg tgcagactat
     3121 aatgagaacg aactgtttat tttgatcaac gacggtgctt acgtgacgat gaatcggaaa
     3181 tttattgttc gaattgacac agaagaggta gaagatccga cagaaaaagc acgttctcga
     3241 aaagatgaga tcatgggtgt agtgaatact ttgtccaata tgggctttta gcaaggatgc
     3301 tgaacaaaga aatacaataa ataaacagaa ttggatttct tcgcttagct tctttgctct
     3361 aggaatacat ttaaaggttg tgacaaaatt cgtgtcacaa tcttttttta ttttgaataa
     3421 gtgatgttca aaaaccattt tctcggtatt aagccaaatt tatgcaaaaa tgtgagaaac
     3481 tattttcaca aaatttttct tcatactcga aactaatcgg cttcttcacg acctcttata
     3541 cacggcgttc aaaagcttgc atctgcgata ataaccccaa ccaaacgaaa aacatgagaa
     3601 gctgtttatg tttggctgtt cttattactt gatgcaggac agctttttta cgacctcttg
     3661 gtacagggta gcttttttac caatttctag ctataattaa agataacctt gaaagttgca
     3721 gaaaaaaagt gcaaaataaa cgataagtac aaaaagacta gttgaaagga cgtcgtcatg
     3781 tcagagcgtg gattattaat cgtgttgtct ggtccatccg gtgtaggaaa aggcacagta
     3841 cgaaaagcaa tttttgagag tgaggataat gattttcagt attcgatctc aatgactacc
     3901 cgtaaaatgc gtgaagggga ggtcgaaggc gttgattatt tcttccgaag caaagaagag
     3961 tttgaatcaa tgatcgaagc tggagagatg ctggaatacg cagaatatgt cgggaattat
     4021 tatggaactc ctttgtcata tgtcaacaag acattggatg aaggaaaaga tgttttcttg
     4081 gagattgaag tccaaggagc aaaacaagtc aaagaaaaag tgcccgatgg tgtattcatc
     4141 tttttgactc cgccagattt agcggaatta aaatcacgga tcgtaggccg aggaacagat
     4201 gcagatgaag taatcgaaaa acgaatgaaa gttgcaaaag aagaaatcga aatgatggct
     4261 ctttacgact atgctgttgt aaatgatgaa gtgccgctag cagttgaacg aatcaaaaac
     4321 attattgcca gtgaacattt tcgagtagat cgcgtaatcg gtaaatatat taaaatgtta
     4381 aaggagatgt agtcttatga tgttaaaacc ttcaattgac tcactattag atcgtgtaaa
     4441 ttcaaaatat tctctagtga ttttagcaag taaacgcgcg catgaattgg atgcaagtgc
     4501 acagcctact ctagattctt ttgattcagt aaaaagcgtc ggtcaagctt tagaagaaat
     4561 cgatgcagga aatgtagtca atgatccaca tcctgaatta aaacgtgaac gtttgaagat
     4621 ggaagaagag gaacgtcaag cacaaaaaga gcgtgaacaa cacgaattgg aatcacgtat
     4681 ccgtgaagag caaaaaatgt aataaagaaa aatagagcga acatctaaaa agaaaagcaa
     4741 acccagctgt ttccttttca aacattcgcc acttattgtc tggtagaaaa ggctgtgtct
     4801 gaactttttg acacagttct tttttttctt atctgctgtt aattttttgt taggatttgg
     4861 cagaagataa gacaaatcca tcgatctcta gtaaaatagg caaggaactt tttgtcgctt
     4921 gataagaaaa gacaaaaaaa ggaggaaacg aaccatgaca ttaacagctg aagtcattgt
     4981 ggacgtacct actatgcaga ccgatcagcc attcacttat ttggttccct ctgaaatgga
     5041 aacagctttg caagtcggga tgcgagtaga agtccctttt ggtaatggca atcgtcatgt
     5101 ccaaggattt gttatggcta tccaaaaatc agaagaatca gctaatccat ctttgaaggc
     5161 agttattcgt ttgctagacc ttgctccggt ggtcaatgaa gaattacttt cattagctga
     5221 ttatatgaaa aaaattactt acgcattcaa aatcacttgt ctacaaacga tgcttccgag
     5281 tgtgatgaaa gcagaatatg ataaattgat ctatcctttg gcagacacac cagaagttca
     5341 ggtattgttt gatcagcgag aagtgatttc ctggaaggaa gcagaagaag aaggcagcct
     5401 ttctcaattg attcgctggc gccaagagca attagtagat atcaaatatg aagtccatac
     5461 acgaaataaa gtaaaaacga ttcgtttggt ccgttctttg ttaactgaaa agcaaatcga
     5521 agaagaatgg gcaaaattac ggcaaaatgc caagaaacaa aaagaacttc ttttgtgttt
     5581 atctgaactg agccaagaag aaccaattgc ttatttcaaa aataaaggaa tcagtactgc
     5641 tgtcttgaat caaggaaaag aaaagggctg gctggagttt atagaatcag aacgttatcg
     5701 cgacccttat aaagaccgag tatttgacca aacgacggca ttggaactga atgcagaaca
     5761 aaaaaatgct gtcgaacaaa tcatcacagc aggtcagcag caaaaagatc aggtcttttt
     5821 gctggaggga atcactggca gcgggaaaac agaagtatat cttcaggcaa tcgctgatgt
     5881 tttgtcagaa aataaaacgg ctattatgtt agtgcctgaa atcgcattga ctccgcaaat
     5941 ggtcgagcga ttcaaaggac gcttcggaga atcagttgcg gtcttacata gcggactttc
     6001 ccaaggtgaa aaatatgatg aatggcgcaa gattgagcgg gaagaagcgc aagtagtagt
     6061 aggcgcacga tctgctattt ttgccccgct gaaaaatatc gggctgatca ttatcgatga
     6121 agaacatgaa tcaagttaca aacaagatga gacaccgcgt tatcatgcga gagatctagc
     6181 aatttggcgc agtaaatatc atcattgccc aatagttcta ggaagcgcga cgccctcttt
     6241 agaatcaaga gcacgagctc aaaaaggcgt ttatcagtta ttgcagttaa accaccgagc
     6301 caaagcagct gcacagcttc cagctattga gatcgtagat atgcgtgaag agtttcagaa
     6361 tcatcggacc tctacttttt ctgctaattt gcaggaaaaa atacaaaatc gattagacaa
     6421 aaaagaacaa actgtcctac tgctcaatcg tcgaggatat tcgtcttttg tgatgtgtag
     6481 ggactgtgga tttgttcttc catgtcctaa ttgcgatatc tctcttacac tgcatatgga
     6541 tacacgttca atgcgctgcc attattgcgg acatgaagaa cctattccca atcgctgtcc
     6601 taattgtggc ggcaataaaa tccgctacta tggaacaggt actcaaaaag tagaagagga
     6661 actccgagaa ctttatccgc aagctaggat tttgcgtatg gatgtagata ccactcgaag
     6721 aaaaggggca cacgaacaga ttttacaaaa attcggtgca aaagaagcag atatattatt
     6781 agggacacaa atgattgcta aaggtttgga ctttccagaa gtgacattag ttggggtgtt
     6841 gaatgctgat acctccctga atttaccgga ttttcgttct agtgagcata cattccaatt
     6901 gcttacccaa gtttcaggac gagcagggcg agcggaaaaa gcaggcgaag tcgtgatcca
     6961 aacattcaat cctcaacatt acgccataga attggctaaa aaacaaaatt atgagcagtt
     7021 ttatcagcag gaaatgcatg tacggcatcg tggaggctat cctccttatt actttactgt
     7081 gaaaataacc gccagccatc ctgaagaaca agtagcggca aagaaaatct ttcagattgc
     7141 caatcagcta aaagaagtgt taagccctca aagcttgcta ttagggccaa cgcccagcat
     7201 gattttgaga gtgaaaaacc gatattatta tcaactaatc atcaaatata aacatgaacc
     7261 gaatcttcct catgttttgg atgaaatttt gaatggaagc cagaaagaac agcgacaagg
     7321 tctttttgtg gcaattgaca acgaaccatt aaactttatt taagaaaggg ccaaattatg
     7381 cgctatccta tattgatcca ccctaatgat aaattaaaaa gaactgctca gccaatcgat
     7441 gtaatcacgg acgagacaat cgctttatta gacaatttat atgagacaat gattgcgaat
     7501 gacggaatag ggatcgctgc tcctcaagta ggacaaaaca agagaattgc tgtgatcgaa
     7561 gtagatgaag gcgagaaatt tgaactgatt aacccagaaa tcatcgaagc aaaaggtgaa
     7621 agccttgacg tagaaggttg tctcagtatt ccgcatgtct atggaacggt caagcgagca
     7681 gatgaagtga ccgtgcgtta ttatgatcga gacggagaag agatagaagt gacggctttt
     7741 ggctatttag cccgtgcctt ccagcatgaa atcgatcact tagacggtat cttgttcatt
     7801 gagaaaatga tacagcagat cccggaagaa gaacttgaag aatatatgga ggaacatctt
     7861 gatgactaag ctagtattta tgggcacacc tgctttttct gtgccgattc ttgaagggct
     7921 gatagaatca ggttacgaga tccaggcagt ggtaacacag cccgaccgtc cagttgggcg
     7981 gaaacgagta attacgccga caccggtaaa aaaagcggct ctgaagcacg gtatccgtgt
     8041 gctgcagcca gagaaaatct ccggttcgcc agaaatggaa gaaatcattg aattggtgcc
     8101 tgatgtgatc gtcactgcgg catttggcca attcttgcct gaaaagttat tgcaagtacc
     8161 aaaattaggg gcaatcaatg tccatgcttc tttacttcct aaatatcggg gaggtgcgcc
     8221 tgttcattat gccatcatga atggggagaa agaaactggt gtaacgatca tggaaatgat
     8281 caaaaaaatg gatgcaggcg gaatctatgc gcaagaaagc ataccaatca ctaaacaaga
     8341 tgatgtaggg acgatgtttg aaaagcttag ccttttagga agaaagctat tgctagaaac
     8401 attgcccaat attttggatg gacaaaaacc agtacctcaa gacgagtcag aagcaacttt
     8461 ttcgccaaac atcacacggg aacaagaagc aatcgattgg cataaaactg cagaagaaat
     8521 tgacaaccaa gtacgcggaa tgcgtccttg gccaattgca ttcacgacct atgagggcat
     8581 acgctggaaa ttattatcgg ttgaagtatt ggcagaaact acagacaaag cacctggtac
     8641 gatcatccgg aaagacaaaa aaaatctatg gattgcttgc ggtaaacaga ccgtcttagc
     8701 catcaatgaa ttacagccag ctggtaaagg aaaacaggcc gtacatgatt tccttaacgg
     8761 aagcggtcag caagttcaga ttggtcagca ggtgaaataa tgaaaaaaat ccctaaaaaa
     8821 atgaaaaaat cagtacgata tatggcatta ctgacattag agcgagtgaa caagggtggc
     8881 gcctactcga atcttttgtt gaatgagatg atcaaacaaa gcgagctaaa tcaaaaagat
     8941 gtgggtttat ttacagaatt gatctatggt acgattagcc atcagcgact attggaattt
     9001 tacgttgcac cattgatcgc taaagcaaaa aaagtggatg actgggtcaa gatgcttctt
     9061 tatctttctg tataccaatt agaattttta gataaagtcc ctgcacatgc tattttaaac
     9121 gaggcggtcg aaatcgcaaa agtaaaagga aatcctggga cagggaaatt tgtaaatggt
     9181 gtcttgagga attatcagcg tcaaggagca ccagatctgc aaggcattgc tgatcctatc
     9241 gaaagacttt ctgtcaaaat cagcctgcct ctatggatga cgaaacgact ggtggatcaa
     9301 atcggatacg aagaaactga aaagctcggt ttatcgctat atcagcctag ccatgctagt
     9361 gcccgagtag atacacgccg actttccaga gatcaggcca tcactgtttt gcaagaagaa
     9421 gagatcacag ctgaaaaaag ccaagtcagt ccatacggta tagtagcgaa aaaagggcat
     9481 ttagcttcta gctcattgtt ccacgatggc gtcatgacga tccaagatga aagttcgatg
     9541 cttgtagcgc cgtctatgca aatcgagcct gaacatcatg tcttagatgc ctgtgcagca
     9601 cccggaggta agacgacgca tattgcttct tttttagatc cgtcatgcgg tggagaagtg
     9661 gtcgctttag atgtacacga acacaaagtc aaactcattg aagataatgc agcacgtcta
     9721 ggtgtctctt ctgctgtcta cgctcaaaag atggatgcta gagaagtcag taaacagttc
     9781 gaagcagagc agtttgatcg gattttagta gacgcgcctt gttcaggtct tgggctgctt
     9841 cggcgaaaac cagatattcg ttataaaaaa cagccggatg aattagcgaa tttaccaaaa
     9901 atccaattgg aaattttaga aagctgcgca ccaactttaa agtcttccgg tattctgact
     9961 tatagcactt gtacgatctt aaaagaagaa aaccaagacg ttgtttctgc tttcttggaa
    10021 agacaccctg attttgagag gattgatgtt ttgaccaatc ctatgctaca atcatcaatc
    10081 gaagagaaaa tgttgacttt gtatccgcat cagttttaca cagatggatt tttcatttgt
    10141 tgtttgcgca aaaagtaatg aggtgaaaaa atgcagattg aatatcaatc agatgtagga
    10201 agacgacgta acacaaatca agactatgcc agtgtattta ccagccgttc aggtctcaag
    10261 cttgctgtat tagcagacgg gatgggtggt catcgtgccg gagacattgc cagtcagatg
    10321 gcggtaacga atctaggcga agcttgggaa aaacaagaag ctgttgacga tgaaaaaatc
    10381 gcccaatggt tcattcaatc gattcaagaa gaaaatatgc tgatttatca gcgtggacaa
    10441 gaacagccgg aatataacgg tatgggaaca acaatcgtag cggccgctct gtcagatgac
    10501 tggttcacga ttgccaatgt tggggatagc agagcttatt tgattcgtga agataaaatc
    10561 atgcaattga cggaggatca ttctttagtc aatgaactag tcaaatcagg agaaatcagc
    10621 gaagaaatgg cagtgaacca tccaaggaaa aatattttaa cacgttctgt cggcatgcct
    10681 ggaacagtcg aagtagatgt ttctactcat ctttggcaac taaaagatcg actgctgtta
    10741 tgttcagatg gcttgacgaa catgctgtct gaagaagaaa taggaacgat catcaatcaa
    10801 gaaggcccat tgtctcataa agtaactgag ctgattgatc gtgccaatga agcaggcgga
    10861 gcggataata tcaccgttct tcttatcgaa tataaggagg acagtgtatg atcgagttgg
    10921 gcaaaaaatt aaatggccgt tatcaaatca ctggaaatat cggtagcggt ggtatggcaa
    10981 atgtattttt ggcgcacgat ttaatcttgg atcgtgatgt agcagttaaa gtcttacgat
    11041 ttgactttca aaatgaccag actgcgattc gacgatttca gagagaagct ttagcagcaa
    11101 cagaactcgt tcatccaaac attgttagtg tttacgatgt tggtgaagaa gacaacatgc
    11161 agtatctggt gatggaatac gtcaaaggaa tggacttgaa acgatatatc caaacacatt
    11221 atcctgttcc ttatgaaaca gcagtaaata ttatgcagca gatcctttct gctatttctt
    11281 tagctcataa ccatcaaatc atccaccgag atttgaaacc ccaaaacgta ctgatcgata
    11341 atgaaggtgt tgtaaaaatc acggatttcg ggattgctat cgctttatct gaaacatcga
    11401 tcacgcagac gaatacgatg ctaggatctg ttcattatct gtcccctgag caggccagag
    11461 gaagtatggc aacgaagcag tcagatattt atgctttagg gatcatcctt tatgagatgt
    11521 tgacgggtag cgtaccgttt gatggtgaat ctgctgtaac aattgcttta aaacattttc
    11581 aagatgatct tccatctata aaagcacttg atccaaatgt gccacaagca ctggaaaacg
    11641 tgattttacg ggcaacggct aaagaacctg cagaccgtta taagtcagcc gaggaaatgt
    11701 ctgatgatct ttctacagta ttatcaccag ctcgtgcaaa cgaagaaaaa tggcagcctc
    11761 atgtgatgga taatgaaaca aaagttatca caccccttac ggaagatgca ccaatgcctg
    11821 attcattcaa atctatgcct ctgccaaaag acaagccaga aaacgaaacc ttaccagagg
    11881 aggaagaagt tctgcctgat cctaaaaaga aaggacagaa aaaatggtgg ctgatcctat
    11941 tagctattct tgctgtttta ggaatcggaa cagctgtatt ctttgcttct ggcgggcgag
    12001 gagaagttgt tgttcctgat gtttctgatc tttctgaagc ttctgctaga gaaacactcg
    12061 caaaagcgaa attagaagta gcaacaaaaa cagaagaaat tgcagatgac aaaatcgaag
    12121 aaggtaatgt agtgaagaca gacccggcag cgggaacgac agtcaagcaa aaacgcgaag
    12181 tcactctgta tattagtagc ggcacgaaaa aaatcaaatt agatgattac agtggtaaaa
    12241 gttatgaaga tgccgaagca agtttgaaag aattagggtt ttctgctgac ttgattcaaa
    12301 aagaagagga attcagttca gaggtagaaa gcggaatgat catttcgcaa tctccatcag
    12361 aagatacaga agtcgatcct aaaacagaca cgattacttt cacagtcagc aaaggaccag
    12421 aaacagtgac tttgagtagt catgtaggag aaaactacga aactgttcgt aattggcttg
    12481 ttacacaagg attcaatatc aataatatta gtgctactta tgactatagc gacactgtac
    12541 catcaggttc gatcatcagt caggatccag cctcgggtga agttgtagca gaagaaactt
    12601 acatcaattt tgtcgtaagc caaggaacag agcctaaatt gaatgatatc tccggctata
    12661 caaaaagcga agcacaaagt tatttagcta gtgtcggtgc agaatatatc gggcatgaga
    12721 cttccgaata ttcaaacacg gtggataaag ataaagtgat tcgtacagta ccaggggcag
    12781 gaacgacaat taccaaagga atggttgtga acgtaattta ctctaaagga ccagatccag
    12841 cgtcttctag ttctgaaaaa gactcttcag acagttctag cagctcaagt agctccagta
    12901 gcacaagcaa ctcttcaaaa gaagaatctt ccagctccac aagttcatca gctagcacct
    12961 cagaaagttc aaaataagtg ctgaataaaa tatatcagaa gggaatgcga cattgatttg
    13021 ttgcattctc ttttttgtgt ttctatctct tttgatcaat tttcaagcca tatactttga
    13081 gaagtttgca tggaataaca cctactttcc tcttcgtatg gtaaaataaa caaaaacgga
    13141 ggtggcactt atcaaaggac aaatcagaaa agcaatcagc ggtttttact atgtatacgc
    13201 aaacggagag acctatcaaa caagaggaag aggaaatttc cggaatcgga aaatcacgcc
    13261 gcttgtcgga gatcaggtga tctttgagag tgaaaatcaa acagacggct atcttctgga
    13321 aatcttgccg agaaaaaatc aattagttcg cccaccagtc acaaatgttg atcttggaat
    13381 cattgtcaca agtatcgtag agcctgcatt ttcttataat ctacttgacc gctttctagt
    13441 taccttggaa tacgagtcga tcgaaccgat tatttttttg acaaagaccg acttgtcaga
    13501 cgacccagaa aaaatcgaag agatcaagca aacctatact aaaataggct atccggtgat
    13561 tgtacccaat catacgggtg atacgacaga actgatccgt tacttcccag aacggctatc
    13621 ggtctttatg ggtcagtctg gagcaggaaa atcgacgcta ctgaatcaaa tatcaccgga
    13681 actgaattta gcaacaggag agatttctga tgcacttgga agaggacggc atacaacacg
    13741 tcatgtggaa ctgattccta tctatggcgg gttagtagca gatacaccag gatttagctc
    13801 gattgacttt ttgacaattg aagcaggaga actgccgaga caatttcctg aatttgtaga
    13861 agcttctgtc cattgcaaat ttagagagtg catgcatgct aaagaacctg gctgcgaagt
    13921 aaaacataga gtagaaacag gtggtatcgc acagacacgt tacgacaatt atttgcaatt
    13981 cttacaagaa atcgaaaaaa gaaaaccaat gtatgcaaaa aaagaaggta aacgaaaata
    14041 ggaggaaaca agaatgaaga tagcaccatc cattttgagt gcagattttg ccaatttaca
    14101 aagagacatt gagttagtgg aaaaaggagg cgcagattac atccacgtag atgtaatgga
    14161 tggccgattt gtcccaaata tcacattagg gccaaatatt gttcaggcaa tccgtcctgt
    14221 tactaaatta ccgttagacg tccatctgat gatcgttgat ccagaaaact acattccagc
    14281 atttgcaaaa gctggagcag atatcattac tgtccacgtc gaagcaactc ctcatattca
    14341 tcgggcactt caaatgacaa aaagcttagg agttaaagct ggagtagtta tcaatccagg
    14401 aacaccagtc tccatgatca aacatgtttt gcctttggct gatcaagtac tagtcatgac
    14461 tgtcaatcct gggttcggcg gacaaagctt tattgaagaa acagtagaaa aaattacgga
    14521 actcgctgat ctacgtagag agaacaattg gacttatgca atcgaagtag atggcggaat
    14581 cgttccagaa acagcaaaaa tctgtaaaga tgcaggggcg gatgtcttcg ttgcaggatc
    14641 atatatctat ggagccgaaa atccagtcgg acaaatcgaa cgattaaaag aggcattaga
    14701 ctaaatgaaa attttattag ctgctggagg taatccgcaa aactggccgc agtctctgct
    14761 gtccaaatac gatgcctatg tcgggataga ccgtggtgcg ctgtacctgc ttcaagcagg
    14821 ctacccagta gatctggcag tgggtgattt tgattcattg tcgaacgaag agcagaagga
    14881 agtatttgca aaagccaaag agtatacccg ttcccctgca gaaaaagacg atacagatac
    14941 acagttaggt atcttaaaaa catttgaacg ttacccgcaa gcggaagtca ctttgattgg
    15001 agctacgggc ggaagattgg atcatctttt agccaatctc tggttaggta tggaacctcg
    15061 cttcaaaccc tttgcccatc agcttattat cagagattgt caaaattatt tgtcttatta
    15121 tttgcctgga gagcatatag taagacagat accagatatg aagtatttag cttattgctg
    15181 cttgactccc gtctcgaatc ttacattaaa aggcagcaag tatgtattgg aaaataaaga
    15241 agtagaatat ccgacatctt atgcaagtaa cgaatttctt tcagataccg cagaattttc
    15301 tttttcagat ggtatgattg cagttattca aagcaaagat tgatccataa acaaataaaa
    15361 aaatttgtct ttcaggctaa aaatgacaga atctattatt tgctaaaaaa aagcttaaga
    15421 ggagcaactt accttttgtt tcacggcatt tagtgataaa attgaacaca agagtctaat
    15481 taattcgagg tgtctttgtg aagaagttca atcctaataa aaatattatt attaccttgg
    15541 ttattgttat catcgtggtg acgatactaa gtgtgaccgc tgcgcagcga gcgaatgaag
    15601 gaaagcccaa tttcttccaa tccatcgtta atgacagtgt tgcaatggtt gataaaacaa
    15661 tctctgcccc agtacggtgg gtacgaaatg gagtagattc tgtccataat cttttttcaa
    15721 cttatgccga aaatgagcgg ctaaaagaaa aaatcgatag ctatgatgac gtttcacagc
    15781 aaaacaagaa ttacgagaga gaaatcgaag cgttgaaaaa agaacttgag ttgaatcaga
    15841 cgctgactag ttacgaaaaa gtaacggcaa atgtcattac tcgttcgcct gacagttggc
    15901 aggacatgct gatcgtagac aagggttcta aagacggtat cgaagcaaat atggcagtca
    15961 tgtcgcaaaa aggattgatt ggccgagtga tcgaggtcaa tacggcttcg tctaaaatcg
    16021 aattactgtc atcctctaat gaaagctcca atcattttcc agtacgggta tcttcggcta
    16081 atggcgaagc gtttggtttg cttaaaaact atgatgaaaa gctccatgcc ttagtggtga
    16141 cccaattaac tggtgatacg gatatcaaag aaggggatgt tgtccagaca tccggtcttg
    16201 gagggaattc tccagctaac ttgccgatcg gtacggttat taaaacgaaa ccagatagtt
    16261 atgggctgga tcgggaagtt tatgtgaaac cttatgcaga aatgtatgac gtgtcagttg
    16321 tgacgattgt ccaacggcta gcggaggatt agtccatgtt gaagaaagaa acaatgaagt
    16381 actatctgcc aatcgttttg ttctttttga tgttgataga tggtcattta acaagaatgc
    16441 taggggagtg gtcgaaaggc acctatatgt caaatgccca ctttctgata ttggcattat
    16501 tatgttgcag tatggcgttt gaaaaacgtt atttactgat taccacgatt gttctcgggg
    16561 ctatctatga cgcttactat attggcgtta tcggtatcta tgcagtagct ctccctttaa
    16621 ttgtatggtt gatgtatgta atgaaagacg ttatccatgt caacatcttt actgaatttt
    16681 tcagtatgat catctttgtc acgggttatg aattgtttac gatggtggtc cagttgattt
    16741 ttaaattagc agtagtaaat aacacgtatt ttattacaag gtttttagga cctacactgc
    16801 tgttgaacat gattatattt gtattattca tttttccctt taagaaatta ttcagtgatg
    16861 aataatttct ttttttattt ttaggtcgaa agcgaaaaag aatcgttgtt atcttaagaa
    16921 atgctagact taacgcggct ttttcatgat cattttctga aacgaacagt tttgtcattt
    16981 ttaaaagttg ttttgaaaca tttttgtaac ataactatta tatcgttgac acactgctat
    17041 ggtacaattt ttatatcgtt aagaaagact taaatatatt tagatattta ttaaattaag
    17101 ccagtcggag gaatgaagag tgaaaaagag tttaatatca gcagtaatgg tatgttcaat
    17161 gaccttgact gccgtagcat caccgatcgc tgccgcggcg gacgattttg attctcagat
    17221 acaacagcaa gatcaaaaga ttgcagactt gaaaaatcaa caagcagatg cacaatcaca
    17281 aatcgatgcc ttagaaagcc aagtttctga aatcaacaca caagcacaag acctattagc
    17341 aaaacaagat acattgcgtc aagaatctgc acaattagta aaagacattg cagatttaca
    17401 agaacgtatt gaaaaacgtg aagatacaat ccaaaaacaa gcacgcgaag cacaagtaag
    17461 caacacaagc tcaaactaca tcgatgctgt attgaatgct gattcattag cagatgctat
    17521 tggacgcgtt caagcaatga caacgatggt aaaagcaaac aacgacttga tggaacaaca
    17581 aaaacaagat aaaaaagctg tagaagacaa aaaagctgaa aacgatgcta aactaaaaga
    17641 attagctgaa aaccaagctg ctttggaatc tcaaaaaggc gatcttttat ctaaacaagc
    17701 tgatttgaac gtactgaaaa cttcattagc tgctgaacaa gcaactgctg aagacaagaa
    17761 agctgacttg aaccgtcaaa aagcagaagc tgaagcagag caagctcgta ttcgtgaaca
    17821 acaacgtcta gcagaacaag ctcgtcaaca agctgcacaa gaaaaagctg aaaaagaagc
    17881 acgcgaacaa gcagaggctg aagcacaagc tactcaagct tcatctactg cacaaagctc
    17941 agcaacagaa gaatcatcag ctacacaatc ttcaatgact gaagaatcat catcagctac
    18001 acaatcttca gcaacagaag aatcaacaac acctgaatct tcaacagaag aatcaacagc
    18061 gcctgaatct tcagcaacag aagaatcaac aacag
//
LOCUS       DAZKOW010000059        17869 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000059 DAZKOW010000000
VERSION     DAZKOW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.059"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..325
                     /gene="sgrA"
                     /locus_tag="ACG912_001848"
     CDS             <1..325
                     /gene="sgrA"
                     /locus_tag="ACG912_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0513160.1"
                     /translation="SSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSV
                     LKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSL
                     FHRVK"
     gene            623..979
                     /locus_tag="ACG912_001849"
     CDS             623..979
                     /locus_tag="ACG912_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513161.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            1024..2139
                     /locus_tag="ACG912_001850"
     CDS             1024..2139
                     /locus_tag="ACG912_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513162.1"
                     /translation="MKNYHLSCSLKKGNPIEGEEYRIRYFFHLMYWQSFDENHSDILS
                     SIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSITYVPEEMNKFVHPL
                     ITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYLEEDLLDTPDKVQES
                     WGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGTKKKVDAFGKSAEDR
                     DFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCMLVRDIFIKHEQPVIF
                     YIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYPIDKKAYDQTNTQIF
                     YWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2165..2605)
                     /locus_tag="ACG912_001851"
     CDS             complement(2165..2605)
                     /locus_tag="ACG912_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0513163.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            2765..3271
                     /locus_tag="ACG912_001852"
     CDS             2765..3271
                     /locus_tag="ACG912_001852"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFQ0513164.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            3439..5352
                     /locus_tag="ACG912_001853"
     CDS             3439..5352
                     /locus_tag="ACG912_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0513165.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5357..6358
                     /locus_tag="ACG912_001854"
     CDS             5357..6358
                     /locus_tag="ACG912_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0513166.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6560..6766)
                     /locus_tag="ACG912_001855"
     CDS             complement(6560..6766)
                     /locus_tag="ACG912_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFQ0513167.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(7412..8260)
                     /locus_tag="ACG912_001856"
     CDS             complement(7412..8260)
                     /locus_tag="ACG912_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0513168.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            8424..9326
                     /gene="gnd"
                     /locus_tag="ACG912_001857"
     CDS             8424..9326
                     /gene="gnd"
                     /locus_tag="ACG912_001857"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFQ0513169.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            9328..10329
                     /locus_tag="ACG912_001858"
     CDS             9328..10329
                     /locus_tag="ACG912_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFQ0513170.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            10326..10610
                     /locus_tag="ACG912_001859"
     CDS             10326..10610
                     /locus_tag="ACG912_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFQ0513171.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            10643..11440
                     /locus_tag="ACG912_001860"
     CDS             10643..11440
                     /locus_tag="ACG912_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0513172.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            11454..12287
                     /locus_tag="ACG912_001861"
     CDS             11454..12287
                     /locus_tag="ACG912_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0513173.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            12305..13162
                     /locus_tag="ACG912_001862"
     CDS             12305..13162
                     /locus_tag="ACG912_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0513174.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            13184..13693
                     /locus_tag="ACG912_001863"
     CDS             13184..13693
                     /locus_tag="ACG912_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0513175.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            13730..14131
                     /locus_tag="ACG912_001864"
     CDS             13730..14131
                     /locus_tag="ACG912_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513176.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            14243..14596
                     /locus_tag="ACG912_001865"
     CDS             14243..14596
                     /locus_tag="ACG912_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513177.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            14630..15073
                     /locus_tag="ACG912_001866"
     CDS             14630..15073
                     /locus_tag="ACG912_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0513178.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            15090..16175
                     /gene="uxuA"
                     /locus_tag="ACG912_001867"
     CDS             15090..16175
                     /gene="uxuA"
                     /locus_tag="ACG912_001867"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0513179.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            16168..16881
                     /locus_tag="ACG912_001868"
     CDS             16168..16881
                     /locus_tag="ACG912_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0513180.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(17088..17237)
                     /locus_tag="ACG912_001869"
     CDS             complement(17088..17237)
                     /locus_tag="ACG912_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513181.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            17633..17833
                     /locus_tag="ACG912_001870"
     CDS             17633..17833
                     /locus_tag="ACG912_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0513182.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         6243 a         2768 c         3728 g         5130 t
ORIGIN      
        1 gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa aagtaaataa
       61 tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg cagggagcaa
      121 cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc atcaagaaaa
      181 agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta gaaccttgaa
      241 caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa gctattttcg
      301 ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat tgaataaaac
      361 gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg aagttgtagc
      421 tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa caggaacaca
      481 aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag tatctctgaa
      541 aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa gtctaaataa
      601 tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg aaaggcaagt
      661 atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg tgaaggatat
      721 cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac aagcagaatc
      781 gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca agatgataaa
      841 agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt tagagggaac
      901 gattggttat attttactgg attcacttta caaaggtact tatcaatcct tagaaaatct
      961 tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga acttaatact
     1021 attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat agaaggagaa
     1081 gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga cgaaaatcat
     1141 tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt tgcgaaacat
     1201 gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag aatttcgttg
     1261 caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa caaatttgtg
     1321 catccattga taacaaaaga agagtttacg cattacatac tagagccttt ttttgaagca
     1381 aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta ttttatgttt
     1441 ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa agtacaagaa
     1501 agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt caagatttgt
     1561 tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca tttgtattca
     1621 agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga agacagagat
     1681 tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag tggggtagct
     1741 cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat ttttcaatac
     1801 tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt ctatattcaa
     1861 tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc tatgactgaa
     1921 cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat tagcgattat
     1981 cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg gaatggacaa
     2041 cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag gaatgagaaa
     2101 aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat tgtttatgcc
     2161 aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga ccagaaagca
     2221 agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg attttatctt
     2281 cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt ttcaataaag
     2341 aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct attctgtgaa
     2401 caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg ttgtttgtca
     2461 attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag ttctgttcga
     2521 taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac gagcagccag
     2581 aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt atgaaataat
     2641 aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa atgttgtcac
     2701 gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga aagaaggcac
     2761 aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag gggtatgttt
     2821 tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata aaagaatcaa
     2881 aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg ctcaattagt
     2941 gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc acagttattt
     3001 tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt tattaactga
     3061 attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta tagatacgac
     3121 tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag caacgacaac
     3181 ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga aaatttggga
     3241 taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa aaagcagaat
     3301 ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc ctgttctatt
     3361 tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca tataattctc
     3421 ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa gggagcgttg
     3481 gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg agagagcatt
     3541 tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc tggaaatgaa
     3601 acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga gacatctgtt
     3661 actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga agaagcaaca
     3721 gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa agagaatggg
     3781 acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata tttgaattac
     3841 ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa gtcaaaatta
     3901 atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa agagattatc
     3961 aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa cagtgtgttg
     4021 atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac aggcttctat
     4081 ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa aatgatggat
     4141 gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca tggcggattt
     4201 cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta taaggccggg
     4261 tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga ttatgtgacc
     4321 tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt tcgagagcgg
     4381 ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat gatgtctgta
     4441 ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg atttgcatca
     4501 attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact accggatgcc
     4561 ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt tatgggcctt
     4621 ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc tctacctacg
     4681 attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga tgatgtagta
     4741 cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga tcttttatta
     4801 gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta cacgaaacat
     4861 gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta tgtggatgaa
     4921 aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc ttatggagaa
     4981 aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt tgaaggaccg
     5041 actgaaggga tttttaatga gacgatacca actattattt tctattacaa aaaaatccca
     5101 gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg aaagaatgaa
     5161 cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt gaaagtacaa
     5221 aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa acattcgctt
     5281 ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat ttggcgaaga
     5341 aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac cagttatcat
     5401 cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc cgattagctt
     5461 gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc gaccagatga
     5521 tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc cttcaaagta
     5581 caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat taccgattct
     5641 tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc gtgattattt
     5701 ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg aacgatcacc
     5761 ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag cagctaaaaa
     5821 agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt cggcaggtgc
     5881 acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa aagctgatca
     5941 tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt ctggtctgtt
     6001 ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt atgaaaaatg
     6061 tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca aaagtttagc
     6121 cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg gaaatacaaa
     6181 tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg gggtgaaagt
     6241 cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc agtttgattt
     6301 cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca aggagtaaag
     6361 tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa ggagcaattt
     6421 caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg agttagagat
     6481 ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt cacgatctct
     6541 tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa gcaacaaatc
     6601 cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag aatgctggtt
     6661 ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata gcaaataaaa
     6721 tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc agcacctcct
     6781 tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt ttgttaggat
     6841 gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata agcaattaat
     6901 gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa taatagaaaa
     6961 tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc aaaaattcga
     7021 tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat aaaattacaa
     7081 atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta attcaccaaa
     7141 atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga aaagataaat
     7201 tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga ataataccaa
     7261 tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat tgatgttctg
     7321 taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc tttttttgtt
     7381 ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa atagtatgtc
     7441 gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa atcacatata
     7501 gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat cggaattctg
     7561 tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa gaattagctt
     7621 ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca atatggattg
     7681 tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc gtcaataaag
     7741 aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata ggggaacgca
     7801 aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg aaaaaataga
     7861 cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt tgtaacaact
     7921 tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc atagtgagct
     7981 cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca ttttcttgga
     8041 tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt gtaaattgaa
     8101 aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga ctatgactta
     8161 ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg ctgaagtcgt
     8221 tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct cattttcata
     8281 ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga gtaggaaaaa
     8341 atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg tataatgagg
     8401 gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga aaaatgggat
     8461 tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc gacatttcaa
     8521 gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag atctctgaat
     8581 ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct ggagagataa
     8641 ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt attattgaag
     8701 gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag aaaaatgaaa
     8761 tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat caagcctgct
     8821 taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc aaagatgtag
     8881 caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc ttgaaaatga
     8941 tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc caacttgtac
     9001 aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat catggttcgg
     9061 tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat cccaatttaa
     9121 gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt gaaacagcgc
     9181 tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg aatctttcac
     9241 aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg tttggcggac
     9301 atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat cgttaattcc
     9361 agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt tggagacgtt
     9421 catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt acttaaagga
     9481 ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt tgaaaacaaa
     9541 gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga tttaaaagca
     9601 gcaaaagaac accagacaat cgtatctatc atacctgctt tagttgaacg aaatgctgta
     9661 gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca agcacaagca
     9721 agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac gctgtttaac
     9781 aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga aattttgcgt
     9841 aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt atacatcgaa
     9901 agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga tatcatttgt
     9961 ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga agtcgatcag
    10021 atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga tgaagaagcg
    10081 atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt tttagaagaa
    10141 gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat tacgccacat
    10201 atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt cagagatgtc
    10261 gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag caactatata
    10321 aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct caattttatt
    10381 tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac ggcgcgaaaa
    10441 gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa ttagtatttt
    10501 tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt agttatgctg
    10561 aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag ttggcgttgg
    10621 cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa tcgatttcgg
    10681 agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg tcatgtataa
    10741 ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg ctttttttat
    10801 aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg ggttagcaga
    10861 tacactagca aaatggtatg aatctgagct catcttagaa caagattttt tgcaagatga
    10921 atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga ttttgaatca
    10981 ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt tcaaacagct
    11041 ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata aatacgcaag
    11101 aaatgtggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg aaagccatcg
    11161 ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac tagaaggtcg
    11221 ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc cggtttctct
    11281 agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg ctgaatttat
    11341 tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat cactgataca
    11401 gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga cacatggaaa
    11461 cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt atgtcccgct
    11521 ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact ggattagtcg
    11581 tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct ttgcaaatga
    11641 tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct attgcagcag
    11701 ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg attgccgttg
    11761 cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc atcaatactt
    11821 ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct atggcccgat
    11881 caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt gttttcattg
    11941 ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag ggaacagtcg
    12001 ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc gcgacaactg
    12061 tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat tttatgagtg
    12121 ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg attattatcg
    12181 ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca agcaataaca
    12241 attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt aggaggaatg
    12301 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
    12361 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
    12421 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
    12481 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
    12541 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
    12601 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
    12661 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
    12721 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
    12781 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
    12841 cactatgggc cgtaatgcta caggcctaaa gcaaaacact ggtgttcaga cagcgattga
    12901 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
    12961 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
    13021 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
    13081 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
    13141 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
    13201 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
    13261 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
    13321 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
    13381 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
    13441 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
    13501 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
    13561 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
    13621 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
    13681 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
    13741 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
    13801 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
    13861 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
    13921 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
    13981 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
    14041 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
    14101 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
    14161 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
    14221 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
    14281 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
    14341 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
    14401 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
    14461 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
    14521 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
    14581 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
    14641 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
    14701 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
    14761 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
    14821 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
    14881 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgttaga
    14941 agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac aaattgtcaa
    15001 acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa aattagatga
    15061 ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg tatggtgaaa
    15121 aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat gggataattg
    15181 gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt gaggagttga
    15241 aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta gcgatccacg
    15301 atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat atacgaacaa
    15361 tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa ccaatttttg
    15421 gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct cttttatacg
    15481 atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata cacagccagt
    15541 caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc gctcagctgg
    15601 aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact tattttttag
    15661 aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat ccagatgatc
    15721 cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac ttgaagaaaa
    15781 ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga tctttagggg
    15841 caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc atccattttg
    15901 tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg gcacatccct
    15961 ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa gaagggttcg
    16021 acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct atgcctggat
    16081 atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat gaggcgataa
    16141 gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat ctttgatatg
    16201 gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa atcagcacaa
    16261 aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca aacagaaatg
    16321 gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat caaatggcga
    16381 gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt atatacgaaa
    16441 aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac aatcgtggct
    16501 tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct atatgccttg
    16561 tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc agagatcttt
    16621 ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt tgaagactcg
    16681 attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat caaagatcat
    16741 ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt aagtttatat
    16801 gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg atttttagaa
    16861 agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta tttattgtcc
    16921 tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt atctacctta
    16981 tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta ccagtagatg
    17041 cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta tgcagatttt
    17101 tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt ggtattttct
    17161 ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata ttgtatatct
    17221 ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa ggcaacgaac
    17281 gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt tcatagttaa
    17341 gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca aaaagtgaat
    17401 caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa tgaaatttat
    17461 atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat gagtaatcta
    17521 ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg cctatcaaat
    17581 atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat ttatgaataa
    17641 cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg gtgaagatgg
    17701 aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa ctttagaaga
    17761 aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag caacaaacat
    17821 tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaa
//
LOCUS       DAZKOW010000060        16809 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000060 DAZKOW010000000
VERSION     DAZKOW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.060"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..749)
                     /locus_tag="ACG912_001871"
     CDS             complement(36..749)
                     /locus_tag="ACG912_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0513183.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            924..2348
                     /locus_tag="ACG912_001872"
     CDS             924..2348
                     /locus_tag="ACG912_001872"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0513184.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            2647..3354
                     /locus_tag="ACG912_001873"
     CDS             2647..3354
                     /locus_tag="ACG912_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFQ0513185.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            3341..3664
                     /locus_tag="ACG912_001874"
     CDS             3341..3664
                     /locus_tag="ACG912_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFQ0513186.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(3753..4790)
                     /locus_tag="ACG912_001875"
     CDS             complement(3753..4790)
                     /locus_tag="ACG912_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0513187.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5111..6094)
                     /locus_tag="ACG912_001876"
     CDS             complement(5111..6094)
                     /locus_tag="ACG912_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFQ0513188.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(6509..7279)
                     /locus_tag="ACG912_001877"
     CDS             complement(6509..7279)
                     /locus_tag="ACG912_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513189.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7272..8207)
                     /locus_tag="ACG912_001878"
     CDS             complement(7272..8207)
                     /locus_tag="ACG912_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513190.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(8523..10172)
                     /locus_tag="ACG912_001879"
     CDS             complement(8523..10172)
                     /locus_tag="ACG912_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFQ0513191.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10289..12892)
                     /locus_tag="ACG912_001880"
     CDS             complement(10289..12892)
                     /locus_tag="ACG912_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0513192.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12904..13953)
                     /locus_tag="ACG912_001881"
     CDS             complement(12904..13953)
                     /locus_tag="ACG912_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFQ0513193.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13979..14293)
                     /locus_tag="ACG912_001882"
     CDS             complement(13979..14293)
                     /locus_tag="ACG912_001882"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFQ0513194.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14309..14755)
                     /locus_tag="ACG912_001883"
     CDS             complement(14309..14755)
                     /locus_tag="ACG912_001883"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFQ0513195.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(14756..16678)
                     /locus_tag="ACG912_001884"
     CDS             complement(14756..16678)
                     /locus_tag="ACG912_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0513196.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         4994 a         3415 c         2764 g         5636 t
ORIGIN      
        1 aaaaaataag ctctgaaaaa ataattttcc cgagcttatt tttcaacaaa aacagcttca
       61 aaaacaaagt cttgatataa atgtctcgat accgaatact cgaaaggttt accagacgat
      121 agagcaacaa ctttgtctat ctcaatcata aattcacctg gatctatttt taatattttt
      181 gcgtcaaatt cattcgcagt atctcctttg atacgtatgt gcgaagtctt tatttccaga
      241 cccagttcat ttgtgatata agcatataca gatttcttta aatgttttgg ttccaagcct
      301 ggtatgactg ctaatggcat gaatgtttgc tcaatcgagt atggttgaca atcaataaaa
      361 cggcttcgta caatattata tacatagtct tttacactga tacttaattt atcagctact
      421 tttttcgatg gaagttcaat tgagaaagta tggatttctg aatctacgtg ttcttcacca
      481 accacgctaa acaaaccttc gtttggacca agaactcgtg agttatcaat tggtgttctg
      541 acgacttcag tcccataccc acttctactt cgcaaaactc cttcggaaac aagtaagtct
      601 acgcctttct ttactgttaa cttgcttgta tgataaataa ctgccaattc atctcctgta
      661 ggaagtttct caccaatttt atactctcca tttacaattt ttttctttat atctaagtaa
      721 atttccatat acttcgcaat ttttttcaat ggtgaccctc caatcaaaaa catatcttca
      781 tctatacttt aacataaata aaccgataaa atccacatca aaattataca cacaataaaa
      841 aagatatata tatttaaaca aaactattta ctttaagttg taagcggtgt tatcattaag
      901 tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa gctaactttt ccagatgatt
      961 tttggtgggg ttccgcttgg tcagctgagc aagcagaagg aaaaggagaa acaggaaaag
     1021 cagagactat ctgggatcgc tggtttaaag aacaacctta ccggttttat caacgaatcg
     1081 attcctctat tacaaccgat cacatccatc aatataaaga agatatagaa ctaatgaaaa
     1141 aaactgggca taactccttt cgcgtttcta tttcttgggc acgaatgttt ccaaatggcg
     1201 gtaccggaaa agtaaatccc aaagccattt ctttctatcg aaaattattt tccgaaatga
     1261 ataataacgg aatcaaagta tttgccaact tatatcattt tgacatgcct gctactcttc
     1321 aagataaagg aggatgggaa tcacgtgaag tagttgatgc ttatgttcat tacgctgaaa
     1381 cttgttttaa agaatttggt gacttagttt gtcattggtt taccttcaat gaaccacttg
     1441 ggcctatttt aggtacttat cttgaagatt ttcattatcc aaacctcatt gactttaaac
     1501 gcggagcgca agcagcattc ttcactatac ttgcacacgc aaaagcaata caagtattta
     1561 aagaactagc tcttcctagt aaaataggcg ttattctaaa tttgagtcct acttttcctc
     1621 gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc tgatttattt tatacgcgga
     1681 gctttttaga tcctatggtt aaagggaatt tttctaaaaa gcttatcctg ttattacaag
     1741 aatataatca gcttcctgaa gattatacga aagaagatct tgtatgtatc tacgaaaata
     1801 ccgctcaaat tcttggacta aactattacg agccacgtcg cgtcaaatct cgttcttcta
     1861 tggtggatac atctagtcca tttttaccag agtggttttt cgaaccacat atcatgcctg
     1921 gacgacgaat gaacgaatac cggggatggg aaatttacga aaaaggaatt tatgatttgt
     1981 gcatggatat caagaaccat tatggaaaca tcgaagcatt tatctcggag aacggaatgg
     2041 gcgttgcgga tgaagaacgt ttcatggatg atacagggca aataattgat gattatcgga
     2101 ttgagtatat caaagatcat cttgcttact tatggaaggc aataaatgaa ggatgtaaca
     2161 ttaaggggta tcatctttgg acatttattg attgctggtc atggatcaac gcctataaaa
     2221 accgatatgg gctggtttca ctcgacctta ctactcaaaa aagaacaatt aaaaaaagtg
     2281 gcgaatttta caagcaatta agtgatacaa atggatttag atacgatgta gacttgttag
     2341 ttgaataata ttctaaaatt ccacttgaag tgaacacatt agaaaacaag aataatgaga
     2401 aataccaata acaaatactt tttcttcata cttcatttat acttgtcagt ataagagact
     2461 gtgacaaatg tagtgatcct cagaagttag gtctggaaat ctaacttgag aagatttttt
     2521 taaactacgt ccagtctttt ttatttgttt aaaattgaag ttgacaagtc ttcattaaaa
     2581 atttagaata ttcacaagtt tgacgacaat tatgaaaatt tatgtcgtgc taaaggagtt
     2641 ttgttattgg atgaaaagtt agatataaaa actgcgatca aagacacgtt acctaccgtt
     2701 tttggttata tcggtattgg acttgcattt ggtatcgttg ggaaagctgc cgggtttcat
     2761 ccgctggtcg tcacgttgat gtccctgctg atctatgctg gttctgccca atttatcacg
     2821 gtcagcatgc ttgctagcca tagtccactg ctctccatcg ttctctcgac ctttctagtc
     2881 aattcccgaa tgattctgat gagtatgacg attgctcctt atttcaagaa aaatcggctg
     2941 cttcaaaatc tgctgattgg cacattgctg acagatgaaa gtttcgcact gggaatgaat
     3001 aaattgaact atactggaca aaagttaaac tttcgttgga tgaatacagc taattggatt
     3061 tcttatttga cgtgggtggg ttcttctctt gttggcgcac ttttagggaa ttttattact
     3121 gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta tgtttatcgg gttactttat
     3181 cttcaagtca tttctgatag aaacacctct aaacgattac agttgatcct gattggtctc
     3241 actttgattc tggtatatgt cggactgatt tttatcccaa gtaacttagt gattttagtt
     3301 gtaacactga ttgggtgcgg attgggggtg tggatcaaac atgccttctt ttgagtttac
     3361 attgctgact attttaggtt gtactttagc aacttggctt tctaggatcc taccatttgt
     3421 cttactgaaa aaattcgatt tgccgcaacc gttgcttgag tatttaagtt ttgtcccgat
     3481 cgtcattatg tctgctttat ggttcagcag tctatttacc caaaatatcg gacatctgcc
     3541 gcagatcaat ctcgaaaatg ctttggcttc agttcctaca cttttagctg ctattctttc
     3601 aaaaagctta ttggtgatcg tcttggtagg aattctttcc ctttcgttga ttcgcttatt
     3661 ataaataaaa aaacaagagc ttatggcaaa ttactgtcat gcgctcttgt ttttatcaaa
     3721 gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt tgaacggacc actaacaacg
     3781 acttttccta atatatgtcc tgtttctacg tctttggttg cttgtttcaa gttcttcgca
     3841 ttcatcccat ctgaatagac cttgctcaat gtactgcgga taatcccttc atttgctaaa
     3901 gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt catagttata gtctgtttta
     3961 gcgaacatat actcccagtc gaagctgata gattggtttt tccaaacacc tatatctaac
     4021 ggctctttga tccctacgat cgttcccaca tgtccaaatg gtcggacaag gtgtttgatt
     4081 tgatcaaagt agcgggtaat atcgaataat acagcgacat actctacttg atcgattcct
     4141 aattcacgaa aacttttact taagtcgtga tgataatcga ttggatagtc tacacctgtt
     4201 tttttcagcc attcaaaatt cttcggactt gcagtcgcgt agacttctag gccagcccaa
     4261 tgcgctagtt gattcaaaat cgaaccgacg cccccagctc cgttgatcac taagattttt
     4321 ttcccgcggt tggcattttc ttctggagtc agaccaaatt tttcaaataa caattcataa
     4381 gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat ctgaaagatt ttttggtgca
     4441 agagcgacaa tacgttcgtc aaccagttgg tattcttgat tgcttcctgc tcttgtcgtt
     4501 gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt gcacttcatc acctacagca
     4561 acaactttcc cgactccatc aaaccctaag atacgtaaag catttcttat cccgttatct
     4621 tggcgaagtt tcgtatcgac aggattaaca gatactgcgt tgacttttac cagaagatcc
     4681 cgtgaactgg gtacaggtat ctgttctttt tcatcaataa agctttttgg atcatcgatc
     4741 gacaaccctt cataaaaacc gactaccttc attaattcct tttctgacat acgaattcct
     4801 ccttagcttc atcaagttgg agcatccata cagttttcat ttcaatatat atagtatgct
     4861 atttgttcca cttttattca tcataattat tctcttactt aaagtaaact atttgcactt
     4921 ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctctttta aacggtgttc
     4981 aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa tatgaggagc tatttttgtt
     5041 tggctgttct tattacttga tgcaggacag ctttttcacg acctctaatt cacggtgttc
     5101 aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc tttttcaaag gtgtctaaga
     5161 tctctcgtac ttcatcagta gatttcgtat tcatcaactg aactcgcaag tcatttgctc
     5221 ctggaaatcc tttgatatag attttaaaga aacgatgtag tcctgtgatg gaacgcggga
     5281 tgatttctga ataatgatct tgcagatcta gttgcagacg aagtagtccg atcagttctt
     5341 tttctgtatg ttccctcggt tctttttcaa atgcataagg attcttgaaa atcccccggc
     5401 caatcatgat gccatctacg ccatattttt cggccaattc ttctcctgtc tggcgatctg
     5461 ggatatctcc gttgatcgtg atcagtgttt gcggagcgat ttggtcacgt aaagcaatga
     5521 ttttagggat gacttcccaa tgtgcatcta ctttactcat ctctttacgc gtacgcaaat
     5581 ggacagaaag attggctata tcttgatgaa gtaggtgact gatccattct tccatctcag
     5641 acatttccgt atagccgata cgcgtcttga cgctgacagg caaaccgcca gctttagccg
     5701 cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa gccactgcct tttccacgtt
     5761 ctgctacatt agggactggg cagcccatgt tgatatcgat tccttggaag cccatttctg
     5821 ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc tccccaaata tgggcaacca
     5881 tcggctgttc atcttctgta aaggtcaatc ttcctcgtac actgtctttt ccatctggat
     5941 gacagaaact gtctgagttt gtaaactcag taaagaagac atccggtgct gctgcatgct
     6001 ttacgacatg gcgaaataca acatctgtta catcttccat tggtgctaaa acaaaaaaag
     6061 gctttggcag ctctttccaa aaattagtag tcatttattt gttactctcc attctatcaa
     6121 gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat actaggaaac aaaaaaagaa
     6181 gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa aaaagactgt gacaaaaatc
     6241 ttatcacaat tccttttccg gaataaatgg tgttcaaaag cttgcatctg cggtaataag
     6301 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
     6361 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
     6421 agctcaacaa acgacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
     6481 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
     6541 aataccaagt aacggcgtac aaaacaacga tgaatagcac aagtacgatc atcgatgttg
     6601 ctactggcac atcgattgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
     6661 tcggattgac tttcgaaaca gcttgccaaa atggcggcaa catagagatg gcatagaaaa
     6721 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcgtcat
     6781 aagactgagc aaaaatcccg ttgagcagtc ctgctaacga aaacaagatt gccgtcatca
     6841 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
     6901 tgatcgttac gagagagccg acgagaatac tgcgacccac tccgccaatc acaaagcccc
     6961 aaatgatcac atgcgtagga acaggcgcta ccaagatttc ttcgatgttt ttctggaatt
     7021 tttgagagaa gaaggacgaa gaaacgttgg catacgaact agtgatagca gacatcataa
     7081 tcaaaccagg cacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgaccac
     7141 cgatcatttt tccgaaaata acaaagtaca aggatgtcgt gatcacaggc ggaaccagtg
     7201 tttggaccca gatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
     7261 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaaa
     7321 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
     7381 gtcagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
     7441 tgttcatctt caaatgtaca agcgtatcct tcaatcacag gtttggtctc atatggcgct
     7501 aaatcaaaga taaatgtttc aaattgcagt ttggatagaa gctctttcat actagtattc
     7561 tcgatcagtt cacctgattg gatgatcccg atgttgcgac aaagcatttc agcttcttct
     7621 aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg gagaaactcc
     7681 cacatctcgc gtctcaattc aatatcgact cctgctgttg gttcatccaa gatcagcaaa
     7741 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
     7801 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
     7861 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
     7921 tggaccgttt caaagggatt gaaattgaac tcttgcggga ctaatccaat ctgttgcttt
     7981 gctcggacta gatccgtatc cagatcataa ccgaagacat tcacttttcc agatgtttta
     8041 ttgactaaag aagtaatgat tccgatcgtt gtagacttcc cagctccatt tggtcctaat
     8101 agcgcataga aatctccttc tttcacggaa agatcgattc ctcgcaatgc ttcgacccca
     8161 cttccataaa cctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
     8221 ccttatagac atgattcttt attgtatcat ggaatacagc agatgaaaaa acaacttggt
     8281 tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc taatgaaaga catcaatcgg
     8341 acgaagtcag acaacctttt cagaaaaaac aaatcgaaga taaaggatta ctattcatat
     8401 gtgcaacaaa aggagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac
     8461 gtataaacgg cggaagcaaa agccaagatt tttgatccaa taaatacttt cgatcacttc
     8521 gcctatccca atacatgcca ctgccgcttt tcatcaaata acgcggcttc cgtttttgcg
     8581 gtttctacta aagtctctgg gtaatccaaa actttcagca aggcaatcgc atttcgggtc
     8641 cttgaaggtc cttcttttaa tttgaaatca aaactgattc cttttccttc tgttacttgt
     8701 tcttcaaagt ggacattctc acaataattc tttaaaatct ctgtcaattc gatatcatgt
     8761 gtggcgacaa atgctaaaga tggatactcc gcaagccacc gaacgactga agcggaagaa
     8821 gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat ctacgaagca gtagcagcgt
     8881 tctttggaag ccacttggtc caacaaacgt ttgatcgatt tgatttctga aacaaaataa
     8941 ctgtcgcctt caaataaatc atcttcgatt gccatggaag tcaacacatg tccaggttgc
     9001 agggtaaact gttctgcaat cgctgtttgg atcgtctgtg ctagaataca gctgatggct
     9061 atacttttga catacgtaga ttttcctgat gcattggaac ccgtgactaa tgtattcttc
     9121 tgccagttga ccggattcac taccgcttct tttaacaatg gatgatagat atctttcgct
     9181 gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg gcatataagt acggtaattc
     9241 aatattgcag cggctacttc taattctccc agcaattccc acagcttgat ggcttcagtt
     9301 gaatgctttt ctaatttagt gagaacgaaa tgataggaga taaatggcag catgaacatg
     9361 atgttgacat attcaaaaat caactctgct tcactcccac tcttcatacg aaatgaaaaa
     9421 cctaatttag ggatgtctct taatggttta gtttgctgtt tcagctcttc ttgaagtggt
     9481 gtcggcagtt ttgctagctg attggcggct gaaatcgttg aaaccaaata actcatgcta
     9541 ttcagctctg tttctagtgt ttgtttcttc actagataat agaccgtatt gaatactaac
     9601 ccaccgattg ccaaatagat tcctagtact tgtcctaaaa gaagcaaacc caaacccacc
     9661 agcggaagca agccaagaaa aataaataaa cctacgcgtc caatcttttt actttttcca
     9721 tccgctagat attgttttgc aaaattatga tctttttttc ctaaacgagc gaattgatat
     9781 tggatttttt ctctcagttg aggattttcc tgataaaaag caatcagttt ttctaactgc
     9841 tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca acgcttctga acctacacta
     9901 gaataggtac tgttgattcc ttcgaatact tcgaacatat ccaggtcata ccaagtaaga
     9961 tcatcgattt cgctatccca tgaacggaac ttcttctccg tgagccatgc ttccttcaag
    10021 ctttcttctt tatcaaatct cggttgataa ggaatttttt cccatttgct acgaactgtt
    10081 tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta ataaaataac agctgcaata
    10141 ataattaaaa cgataaattc tctttgaccc atatccctct ccgttccttt atttgttagc
    10201 tttagtatag cactgttgcg ctatttgaaa agagggtaaa aaaacacctt tcaaaataaa
    10261 aggtgttttt ttcttgtata ttacatattt atctctcatt tatcaaaatt gacaaataat
    10321 catagggagg aattgttgtt aattcttgtt tattctccag tgcattgaca actgtcccaa
    10381 gggaaaggat agcctcaggt actatttttt cttgttctgt tggattagct aagcgaagaa
    10441 caaatccttg ttcatgaaaa taagaaggat aaatcgatga tacaattaag tcttcaggta
    10501 attcaatcaa tgaaaattta cgaggaacat caagttcatt ttgtaatggc caaatcttgt
    10561 tatctaacct gtgaataaat acattcagtt tctgcttttg ataagaaata gattgttcta
    10621 accgtttttc tgcctgtttt gccactttga actcattaaa tcttccttct tctacattaa
    10681 atccaaaagt gactttatac tcacctatct cttgagctag cggtgtttcc atcatgacat
    10741 gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg ccaagcaaga ttaggttttc
    10801 caagttcccc tgttgttgaa aataaggtta tgattaattg attttctata cgttcatact
    10861 cttttaaacc atctgcataa aacgtaaaat aatgatcatt ttctgccaca gagacactct
    10921 tatcgaatat ttctaaatta acaggtttct ctaccatagt ttctgaccaa ttcggaggaa
    10981 tgacatcagg ggtattttca ataaatccgt tttgaatttg tgcaattgat ttttcagatt
    11041 tatctaaaac attaattgct aagcgcatac gatgcgagta aacagtatta tgaattttga
    11101 gtgtgccttc aacgatttga ctttctccca gactgaacga tatttcataa gatatttctt
    11161 ccatttcggg tttttcagat aaacgatctt ttaaatcttt aggaagttga gcttttccgt
    11221 aaacaactaa tgtttcttgt aaagaatctt tatatacttt agctgtttct aattttagag
    11281 atagttctgt atctccttct aatggtgaat aatcatacgt atcgccatca tttgctgaat
    11341 cgatcaaatg aacgaaatca agatattgtc tatctccaac tatcaaattc aattgcccat
    11401 ctttaaaaga aagtttatat ttattgttac ttatctcagt tttgtctatc tcgattgctg
    11461 tttctaattt ttcattattt ttctcaaatt caacaacagt gtatcctagt gctggcacat
    11521 ttacatttat ttttatgtca agttcataat aagctggttc atcaaaatat tcgaaacctg
    11581 tagcaatcat ccgataagca tttttccgtt ctggaaaata aacttctttt tcaatgatat
    11641 tatagggact acctttgaag tgtattttct ttgaatcagc tactacatgc acgattttcg
    11701 atccactata attctcagtc gttgtgttga aaacaaggac ttcattcgat tttagcttta
    11761 atgcatcagc catccgtttt acaataaggt tttcgattcc gtcagctatc tcattagctt
    11821 ctttgattcg atggaaaata tcttccgcaa cattatctga aacacagcca ccaatactat
    11881 catgagcctg gttttgcaaa atctttttcc ataaccgacg aagtaggcct ttactgatat
    11941 ggataccatt tctttctgca atcagcatga gcggctcaac tcgttttaga attttttgtt
    12001 ccaatttaaa attttctatt ttcatttttg ttcttactga gccaatagaa cgatgaacac
    12061 gagagtaaac cggctgacga agttcacctt tatattcagg taattctttt ttgtttctga
    12121 taacttctac ataagaagga taatctgaaa taatattgtg gaatttactg tatttattca
    12181 acttctctac gacttgatca aaatttaaaa tcattgactt ttgatctaca cccgaaggta
    12241 ctaaaatatc tttgtttttt ccttgcatat gaagcatttc aactgcttca tccaactttt
    12301 cttttgcaaa ttcaggaata tctttaattg cgtctaaggt catcattcca gtcatataac
    12361 caaatggaaa attcatggca taaacaaatt tatctcccaa tcctttccag ataaaataag
    12421 gagatggaac catcgtttca aagtttaatc ctcgccaaca catgaaatta tcgatttcta
    12481 cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa cgtgtcaggc aaatacccta
    12541 ccatcattgg atctccatat ttaaggatcg tatcactgat tccaatcatt aagtttctta
    12601 aaattgattc agcatccact aataaggcat ctgtttgtgc ataccacggt cccacgaata
    12661 gttgtcctgt tttcatcagt cgttggataa ccttaagctt ttgcggattg atctccaaat
    12721 actcatcgac gattgaagtt tgtccatcta aacaaaagtt tactttaggg ttcctttcca
    12781 attcatctag aacttcagta aagacctgat cagacaatac gatggcatct tcatttgtaa
    12841 aataccattc tctgtcccag tgcgtgtgtt gaattatatg tgcatttacc atatcagatt
    12901 ctcctacttt ccttcttcta ctgaatttac ttgttgttct tttttatact tgatggattt
    12961 tagtgcattg accattaacg ctgtaaccac tcccccaata acaattgata ctaaatataa
    13021 aggtatattc gtaacaaaag gaattgccat aaaggttgaa agcattgttt cttgagtaac
    13081 accaaaaaat ccatttagac cacatgctac tgcagtgccc accacgatgc ttggtattac
    13141 tcgtaaagga tctttcacgg caaacgggat cgtcccttca gtaatcccca caaggcccat
    13201 aatcatagct gtctttgcat tatctcgttc cgattcatca taaagttttt tgtttaaaat
    13261 tgttgctaaa aacattccaa atggcggaat agcaattcct aataacatgg ctgtactagg
    13321 tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca tatgcgattt tattgatagg
    13381 tcctcccata tcaaacgcca tcattcctcc taaaatagcc gacatcaaag cagcatttgc
    13441 tcctgacatg ccttctagcc aattagtcag tcctgttgtc attgcttgaa taggcgtagc
    13501 tagtacatac cataatatga ttccaacaac taatgtagta acaaaaggaa tcagcataat
    13561 tgattttaga ctaataactt gcatcggtac ttttactttt ttcaatgcat tagctaaata
    13621 gccacaagct aatccagcaa ccattcctcc aataaatccc ataccaagat tttgacacat
    13681 catcccagtg ataaaagctg gtgctagacc gggacggtca gcaattgaat atgcgatata
    13741 ggctgaaatc atcggtacga taaactgtaa tcccgtctga ccaatcattc gcaggactcc
    13801 ccagaatgta tccgtctggt cccaaacacc tgtgccacca aaaatgactg caatagaagt
    13861 tagtaaccct gctgctacga caagcgggat catataggaa accccacttt ggaaatgatt
    13921 tagaatttct cttccttgtt tcttgaattc cattagtaac tcccccacga tatatttttt
    13981 atttgttaat taatgcaatt gcttcatcaa tcgtctcttc accatctttt actacctctg
    14041 aggtccctac tgttaaaatc ggtttaccgt caaagcgacc tcgttctgaa acatttgtat
    14101 ctactgcaaa tatgacaaca tctgcctcgg aaacatccga aattgaaagt ttgttttcta
    14161 tccccatagc cccctgtgtt tctactttaa tatctaaccc tttcttcttc gcaaactttt
    14221 ctaaattagc ttttgccata taagtatggg caatccctgc gatacatgct gttactgcta
    14281 ctatttttgt catatgaaat tcctcctttt aaaatgatag agcttttacc aattcttttt
    14341 tatttctcga atcttttatt cttttaacaa actcgtcatc catcaatctt ccagataatt
    14401 ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc ttttattgct aacaaaaaga
    14461 cgagatcaac tggttgatca tctaatgcgc cccattcaac agaccgcttt aacttggcta
    14521 cagccactgt tgattcaatt actgctaggc ttttgccatg aggaatggcg atatttttcc
    14581 caattcctgt ggtcatatgg ttttctctgt caaatacgtc tttttgaaat tgtttgtggt
    14641 cagacacaaa cccattagtc tctaacaatt tcgataattt ttcgactaca ctttttttat
    14701 cagatgcatt taaatctatc actatattat ttatattgat tttttcactg atcatttata
    14761 agccttcttt caatatataa actaattttt caaacgacga actcgttttt aaatttgcta
    14821 ataactcatg attcgaagcg atttcattga atgtttcata aattttattc aattcaggtt
    14881 ttactttttc attaagtgca aaaaagaaaa cagaatgaac aagtgtccca tcccaatcaa
    14941 ttccttctgg attaacgtag acacagatta ctggcttttt tataaaatct acttcgatat
    15001 gcggcatagc aaaatcattc atcgctgttg aagatagttt ttctcttttg attgctccct
    15061 ctagtatccc ttctttcgcg taattttctt ttaccaattt tttggtaatt atatttaaaa
    15121 cgttcacttg attatccttt atttggtggg taaaaaagca aaatcgttta tctaataaat
    15181 ttagaaaagc atcttctgtt tgttcattta actgtagata ctgttttact tttctgacat
    15241 ctgcccctaa catcaaagga ctaacaacaa taacaggaaa atttgtattt tcaatcggaa
    15301 tcgtgcttat aacaagttca tcagtgactt ttctatcttg tagttcattg atgcttagta
    15361 catcttctat ctcaaccata tctgcaaaac cgttatttat tctttgctct aataattttg
    15421 atgttccata tccgctacta caaactaata tcactttcgt tttgtcaggt ttgtaacgat
    15481 ccaaaagtga ttgaatatgg agtgttacgt aagcgatttc atcttcatta aaatgtatac
    15541 agaatctctc ttcgatttcc tctaataaat ctacacttat cataaatgac cttttaaaag
    15601 aatatttgat tttgtccgta taagggttat agatatttac tcccaatttg agtcttttaa
    15661 ctgctgcatt gagatgtaat actagattat tgatcaattc ttcatcagga taaaattcat
    15721 ttaatttaat tcttactaac tcttgtagct caacacccat atctaaatag gaaagcttcg
    15781 tttgatctaa gtcgtgattc atagcatttt tttcaatcga agtaatgtga atgtcaacat
    15841 aagcttgttc atctattggc acaataattg aaaatgcttt ttcaatcatt ttgattaaaa
    15901 taagtgtatt ctttgagatt tcttcttcag aattattact acgtgataag aaattgcttg
    15961 tttcaatcct ttctatcgca atagcaaggt ggatagctaa agtttgaaat tcataatctg
    16021 taaatttcaa ttgtgtctta ttcatgtatc gattaagcaa ttcggctact tttttcaata
    16081 tgtccttatt taagaaatta ttcgcttcac caatgaaatt gatattcaag tctaagagat
    16141 ctgttttact gttagatgct gtaaagccac cccaataata gttcacaact tctgacatca
    16201 accttctttt acttttttca tcagctttga tgcgcacgcc atttcgatct gaaactaagg
    16261 acatcccatg cttgtttagt ctttttctaa catctttcat tgtgctttct accgtcgacc
    16321 tgctcacgta taattcgtca gcaagatttt gaatctttag agaatcatta gtattcaata
    16381 atttagcaaa cacataagtt agacggtcct ccggagagtc tgcgatattg attttatgac
    16441 tggataataa atttcgaagt gtttcttttt ttccaataag cgaaacgcct tttttaggtt
    16501 gaaccaacat ttgtacatct ggcatttttt ggattatttc tttcaaaatt aaaatatctt
    16561 tgctaattgt tttcgtagat acatataatt gttctgataa ttctttgtaa gttgtgtgct
    16621 cattttcaat caaaaagtat agtattttca tttgccgttc gctaaattcg atgcccatta
    16681 tcttcacctc ctgaatacag cttaagtgta caattattat ctctattttc atacagaaga
    16741 aagttcttga ttaatgtaga agtttctaat attcttgatt atttcaaatc aataaaaaag
    16801 cctgcaata
//
LOCUS       DAZKOW010000061        16799 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000061 DAZKOW010000000
VERSION     DAZKOW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.061"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..384)
                     /locus_tag="ACG912_001885"
     CDS             complement(<1..384)
                     /locus_tag="ACG912_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513197.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKI"
     gene            complement(569..967)
                     /locus_tag="ACG912_001886"
     CDS             complement(569..967)
                     /locus_tag="ACG912_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFQ0513198.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(964..1737)
                     /locus_tag="ACG912_001887"
     CDS             complement(964..1737)
                     /locus_tag="ACG912_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0513199.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1734..2417)
                     /locus_tag="ACG912_001888"
     CDS             complement(1734..2417)
                     /locus_tag="ACG912_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFQ0513200.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2584..3471)
                     /locus_tag="ACG912_001889"
     CDS             complement(2584..3471)
                     /locus_tag="ACG912_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0513201.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3468..4337)
                     /locus_tag="ACG912_001890"
     CDS             complement(3468..4337)
                     /locus_tag="ACG912_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0513202.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4474..5760)
                     /locus_tag="ACG912_001891"
     CDS             complement(4474..5760)
                     /locus_tag="ACG912_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0513203.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            6033..6611
                     /locus_tag="ACG912_001892"
     CDS             6033..6611
                     /locus_tag="ACG912_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0513204.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6611..7540
                     /locus_tag="ACG912_001893"
     CDS             6611..7540
                     /locus_tag="ACG912_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513205.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7537..9273
                     /locus_tag="ACG912_001894"
     CDS             7537..9273
                     /locus_tag="ACG912_001894"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0513206.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG912_001895"
     CDS             complement(9336..10172)
                     /gene="pheA"
                     /locus_tag="ACG912_001895"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFQ0513207.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(10169..10678)
                     /locus_tag="ACG912_001896"
     CDS             complement(10169..10678)
                     /locus_tag="ACG912_001896"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFQ0513208.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG912_001897"
     CDS             complement(10671..11966)
                     /gene="aroA"
                     /locus_tag="ACG912_001897"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0513209.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11979..13070)
                     /locus_tag="ACG912_001898"
     CDS             complement(11979..13070)
                     /locus_tag="ACG912_001898"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFQ0513210.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG912_001899"
     CDS             complement(13087..14253)
                     /gene="aroC"
                     /locus_tag="ACG912_001899"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFQ0513211.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG912_001900"
     CDS             complement(14255..15319)
                     /gene="aroB"
                     /locus_tag="ACG912_001900"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFQ0513212.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG912_001901"
     CDS             complement(15352..16374)
                     /gene="aroF"
                     /locus_tag="ACG912_001901"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFQ0513213.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4858 a         3708 c         2970 g         5263 t
ORIGIN      
        1 aatcttttgc tgacgtctaa aaatagaaaa agaattttcg tcatctattt gataacgcat
       61 ttgtttttgc agttctgctg cagtcagatg gatcttccca tcgtttgtat cgatcattaa
      121 accttctgga aacagttcaa ccactgctcg atcccaagat tcaagagaaa gctcgatata
      181 atcttttact ggtaattcca attctacctt aaaaaagtgc aaaacagccg acagcccttc
      241 tgcttgatag atttctccta atgttagccc ctctagatgg aactctggtg aaaaatgcag
      301 caatgtttta ttttcctgca attgaacata tagaaatgta gatcgctcaa tcagagtatc
      361 ttttaaaaca actaaaaaat tcatttttct cccccttttt tcatcatttt taaaatagca
      421 tattttgaaa agaaagtctg atgcttagag tagaatttca taaaaattta ctatttcata
      481 agtattcacg agaaaaatac aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt
      541 tgtgtttatt tcctcttcaa acgcttgtct atgattcaga cttttctcta tcgttatttt
      601 catctaaaag ttcttgcatt ccgataattt ctcgatattg atgaatctta gtgataccaa
      661 tgactttgtc tacatcaata ctaaaacaaa taccattacc tggaatatcg atgttgatgc
      721 cttgaccaat cgcatccatt actttattcg tcaaactatc gggtactaat gtcaacacaa
      781 tatctttttc tggcgaaatg tccaaaccta aaaatttccc tgaatcatgt acaccagaac
      841 ctcttccatg taagatcgtt ccgcctgctg cacctgcttc ttttgaaaag tcgataacat
      901 cttcgcccat tcctccgtca acgatcgtca caatcatgtg aagattcaat gctacaagat
      961 ctttcatttg ctttccccca atttttgcaa tctttctttt tcgattctgt ctttttcatc
     1021 ttttcgttta taaatatatc ctagtactaa agtggataat attggtgtca atgcaatcat
     1081 tgcaaccaca ccaaacccgt caatgatcgg atctcttcct tcaatttgtg cagcgattgc
     1141 cacgctcatt gataaaataa atgtcgaaca catcgggccc gttgctactc caccgttatc
     1201 aaaagccatt gctaaaaaga tcttgtccac ttttcgtcct aaaataaatg ctagcccgta
     1261 tcctgggatc aagaaataat aaagagagaa tccgacgagc aaacgccaca tagacaatcc
     1321 tactgctgcg ccgatcccaa ttgaaattac catcagcatg attttcgatc ggattctccc
     1381 ctcgctcaat tcttcaacct gtttgatcat tacatgaatc gccggctcag caaaccctac
     1441 taagaacccc attaaaaagc caaggggaac cagtatataa ttgtaaggca atcccgcaat
     1501 agcactgcct aaatgctgtc ctaccggtac ataagccata ttgactccgt gcaggaaaag
     1561 gatcaaccca atagtcgtta tcaagaaacc tttcataatg tcaaagaaac gctttttgcg
     1621 catacgaaaa ctaatgatat tcattaaaac aaataggatt acgataggtg ccactgccaa
     1681 aatgacttcc atggtaacga tttcgattcc atctatcact tgctgccaga aactcattgg
     1741 aagatcaccc ccaaaatcat cacggcaaga atcggcccta acgaagcaat accgaccatt
     1801 ccaaaactgt cattttcatt tttcttttgt ttgatcatac tagtcatccc tccggcaagg
     1861 gccaagataa atggaacagt tactggacca gtagtcactc cccccgaatc aaaagcaatc
     1921 ggcataaatt cggaactggt gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc
     1981 atcaattgat aataggatag cttgaatacg acccgaagca gcgcaaaggc tagaaatact
     2041 ccagtgccga cgctgacgat cccaatcagc aacactctgc caatctctcc gtcagatata
     2101 tcatatactt gttgtcccaa aacttgcacg gaaggttcag caatcgtgat cacgatcccg
     2161 atagcaaatc ccaaactgac caaaacggtc aaacttttct ttttgatcat atatttccct
     2221 actagatctc cgacttccat catagaataa tctgctccaa acaaaaagag cgtcattccg
     2281 atcatcatga ttgccgcacc aaccagaaat gaagcgaggt cttctccttc taacggtgca
     2341 aaaacaaacg ttaaaatgac aatcaggaca gtcatcggca atatagcagc aatcacttct
     2401 ttaaaatttt ttagcatgta tcctcctcct ttactcttta agtttacctt aaaatgaaag
     2461 agttttcgag cacaaactta aaacgttatc atttaatttt tctcaacata aaataacaca
     2521 cgtcccaaaa gtatcttttt acttggaaaa atgactttga aggaacgcgc ttattgtgtt
     2581 tttttatttt acagagccga tcattccttg aacgaattgc ttttgcaaaa caaaaaatac
     2641 gatggcagtt ggtaaagtag cgatgactaa tccagccatg atcattccat agtctggtgt
     2701 ataggaagca cccatagcag ataaaacaag cggaaccgtc cgtttatcag gagattgcaa
     2761 agctactaag ggccataaat aattgttcca gctactcatg aatgtgatga ttgcagccgc
     2821 ggcataggta tttttagccg tcggcatata cactctaaaa aaaatactta gttcttttag
     2881 accgtctaat cgagcggctt ctactagatc ttttggaaaa gatttaacat tttgtcgaaa
     2941 gaagaagatc aaaaaagcag tgctaacaga tggcaggatg acaaccaaga aactgttgat
     3001 tcccaaaacc gttccgttca attgtgaaaa cattcgatat aaagggatca tcaatgccgc
     3061 aaaaggaacc atcatcgata gcagtagaat attgaaaatt aggtcttttc gtttactttg
     3121 ataaatctca aatccatatc ctgccattga cgaaatcagc aatgcaaaca cagtggtcac
     3181 caaagcgatc atcgctgaat tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa
     3241 attttgaaaa ttgatgatca agttactgcc aatctttatt tttcccgatg tgatatccac
     3301 gggtgtattc gtcattccca aaaccatcca gacaaaaggg aaaatggaaa taaatgatag
     3361 gatgcttaat gctgtatact ttaatactga cccaattgtc gaacgaagtt ctaatgcttg
     3421 ttttttgacg ttggggcgtt cgattgtttc tttttctacc atatttttca tcgactttct
     3481 cctcctactt tcgtttggat tatcgacaat atcacaataa aggaaacaat aacaaaggaa
     3541 actgctgccg catatccaaa attaggcgtg aatttataac tcaaattata gatgtattga
     3601 gatagggtgg tcgttgcatt tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa
     3661 agctgcaatg tcccgatcgt cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt
     3721 gggatcgtca ctttgataaa ctgctgccat ttagaagcac cgtcaataga tgctgcttca
     3781 taaatctccg aatcaatatt ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc
     3841 caacgccaag tgatcgaaag aatgatcaga actctcgccc aaattggatg agtcaaccag
     3901 ccaatcggtg tgtcaatcat accaatgccc aacaacaagt tgtttacaag gccagattga
     3961 gaaaatagac ttttcatgat cagagaataa ctgaccatcg aagtgataca tggaagaaag
     4021 atagcggttc gaaacaaccc tttatatttc aactttttat catttaaaag gttggaaata
     4081 aaaagtgcta gaaacaacat gatcggtact tgtacaagta aatacaggaa tgtattaaaa
     4141 aaagcttttt taaaagttgc atctgtcaac atccgctgat aattacctaa cccgttaaat
     4201 gtcatattga cagggggacc actttgaaaa gacgtgatca aagccaaaac catcggaata
     4261 aaaaccatga ttgatatcaa aatgactggc aagattaaaa aagcatgccc gtttttgaat
     4321 aaatctttct gcttcattct tttccctcca attctatcat tgattatcta catgaaaaga
     4381 caagaaagac aatatctgta aggcagacgt gatctttttt gtcttttcgt ttgtctgatg
     4441 aaaaagcgaa gaaattcact tttttaccac cgatcaatta gccaattgtg cttctacttg
     4501 tttttgtgta tctgctagta ccttgtctgt ctcttctcca ttgatgattc gatgaagagc
     4561 ttcagctacg actgcttcaa ttgcataggt ctcatgtccg taattcactt ctggtatttc
     4621 agatgtccat gttgaaaaat cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc
     4681 ttgataagct tcttgttcat tcgcgctaag catcgtagat actagtccga tttctttcgc
     4741 aagagtgccc atcagttctt cattcgaagc aaacgttgct tctaggaaat ctttcgctga
     4801 atcttctcca gctactcctt tgatcacata ccagccagcg ccgccaaggt tagaagcttg
     4861 cgctttttgc agattttctg gaagtgcagg tattggtgca atcttccatt ttccagattg
     4921 gttttctgct ccttggatcg ttgaagaata ccaagcgcca gttgggctag aagctactgc
     4981 tcctgattgt atcgcagtta ctccagcatt ccagtcagag gtttgttcga ctagatcttc
     5041 tttcaaaaga gtggcaaaca attcaagacc atactttaaa gatgcattgt cttggatcgt
     5101 gactgtctcc ccatcttcag atgtatacca ttctcctgcc tgttgcataa tcatccttac
     5161 tcgaccaagg tcagaaggat tgacttcagc gattttcttt cccgtctttt ctttgacatc
     5221 tcgcgctact tgaatatact cgtcccactt cagattctcc atatcctctt ctgtataacc
     5281 tgcttctgcc atcagatccg tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac
     5341 accgtatatt ttatctccta ctttgtttac cgcaaattta tacggtgcaa aatgatcttc
     5401 ctttacaata gcagacaaat tagcgaatgc atctggataa gaagtcaaat agccttgtat
     5461 cctataatct tcaatcaaga cgatattggg caatccttct gtattcccgc tcgctaaagc
     5521 ggtgttcagt ttctgaacga tatcatcctg agacatcgta acaacttcta tctctgtttc
     5581 ttcgttctca taaacttttt ttgcttcatt cactgctttg atattgaatg tctcatccca
     5641 cgcccagaca gtgacttttt cttctttgtc tgctgtacta ctagcacttc cgcatcctgt
     5701 taagaaacct gatgctgccg ctaaacaaac gactccagca attaactttc tgatgttcat
     5761 ttttatccgc tccttttcca tccattagtt gtttacgttt gctacattca tcataccaaa
     5821 atttgaaaac ggtttcttta ttattcagat acttcaaaaa tttctcctaa aataataaaa
     5881 aaatacaaat caactatgac aaaaggaata atcttagcat ctagaccaaa gatcgaaatg
     5941 gtgaggtttc tttaaaataa aaatatcctt gcattttctc ctagaaataa gtatttttaa
     6001 attacaagac tattcttaag gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg
     6061 atgaacagac gattttagat ggaatgaaac agttgatcaa ttggtctgag cacgggtttc
     6121 aattaatagg gactttttcc agtccgctga aagcgcttga tttttctttg gtacatgcaa
     6181 tcgatatcgt ggtcacagat gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc
     6241 tattaaaaca gcaacaacct gaaacacaga tccttatcct ctctagctat gatgaatttg
     6301 atatggttaa agattctttt aaagaaggcg tcgctgatta tcttttaaag cctcgattga
     6361 gtcctgattt ttttctaaat tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca
     6421 acaaaagccc aatcaccaat cgttatgagc agatctgcga gaatctttca cagcagattg
     6481 ctggacaaac tcgtccattg cttgaccctc ttctttttga acacaccttg tttttctgtt
     6541 atataccgaa aaacggccat tcagttcaaa acaaggctct aaacaaaaac agtcgatcac
     6601 ccattcattg atggatagcg acgattcgtt tactatttat cctgtttcta ccttttcgga
     6661 agaatccggc tatgtaatca atactgattc caaagataca cttaggaagt ttgttcaaaa
     6721 gtttcgtcaa gaagtttttc caggtgattt ttttgttgtt tctgattctt ttgcaatcca
     6781 agatatcaat gaaatattcc aagagatcag aaattgcacc aaaggacaac gtttctatca
     6841 taagggaaag aaccttgctt atcagcatga acttcttccg ttattaacca accacgagca
     6901 gcaagcaagt agttatttaa caaaaatcgt tcataaagac tacgctttag ctgtcaaaga
     6961 acttgagaat ttcttcacga cgatattgga acagcctatt gatccagctt atttgaaaca
     7021 tgaggcaatc aatcgtttct atgcattact caacgctttg gctgacgagc atgttgggaa
     7081 tgaagaactt tctttgctca agatcgccct tccttcttta ttagccgatg ctgagtattt
     7141 cgaagatttt tctattcttg tcaaagaaac acttgagaaa ctcacccact tttctgcaaa
     7201 tgcaaaaaaa gacaatttcg atatcttgac tttgatctac gagttcgtcc aggaaaatta
     7261 tgagaaagaa atcaatttac aacttttatc cgaaaaatat catttttcat atagctatct
     7321 ctcagccatc tttactgaaa aatttggtat caacttttca aaatatctaa aaaaagtccg
     7381 tatcagtaaa gccaagctct ttttgacgga aacacggctt actttgacgg aaatctgtga
     7441 gaaagtcggc tataccgaac tgggttattt ctccagagtt tttaaagaag agacaggaat
     7501 gacaccttct caataccgta aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat
     7561 gaactctttg ccaaacttct gttcattttg ttcatcggtc tttttctgca agccatcgtc
     7621 atttctttgt ttatttatca tcgatcaagg gatgcctata tccagctgtt caatcaatcg
     7681 aatgatgtgg tactaaaaaa gattcaatct gaatttgaaa gtttgaatga tacaatcgag
     7741 aatacattag ccgcttttga ttctaatcca gctgtcaaaa gctatttttc caatgatcca
     7801 gcccaacata tggaacagct tcagcaacta agaacaatcc aaaaaatgaa tgactctctt
     7861 tcaaaaatcc atccaatgat cgattacgat gtattgatct ttggagaaaa tggaagaact
     7921 tttgtcggaa acgacatgct cactgccgta tctgccgatt ctttttttca atctgcgatt
     7981 gctcagagag tgaatgaacg tgctgctgat acacaaatgc tttttgcaca ccacggattg
     8041 actcttcgcg ataagaaagc acccagcgtc ttctttgtca aaaaactgaa aaatacattg
     8101 aatcacgttt acggatatgc tgttttatcg atctcatcca agcaactagc gaatcttttt
     8161 caatctgtag taaatcctga gatatcgaag atctccttca tgaacgaaga acagacgatc
     8221 atcgcatcaa atgaagaaaa tacaattggg agccgatcaa tgattttcaa aaaactaaca
     8281 gtaggtgaaa caacgatttc aaatggacag cagttgaccc gtcttcctct gtaccgccag
     8341 gattgtacat tactcagtca agtcgatctt cattctttat ctaaccaaat gggcgtgatt
     8401 tttccgatta ttttgtataa tatcgtgaca cttgcttttg ctggctcatt ggtatttatg
     8461 tatttggatc gacacacaaa atcgatctac cgcttgattg attcgctaaa acggctagag
     8521 gaagatggct taaaagctac tgttcctgta caaggaagcc atgaagtcca attgctaggg
     8581 aatacgatca atcaattgct aagtaatgct cagcatcatt atgaacagac gatccaagac
     8641 gaaaagaaga aacgttcgct tgaaatccaa actatgcaag cacagataca gccccatttt
     8701 atttataata cgctgactag cttgaaattt ttgatttggc agaaaaaaac agatgaagca
     8761 gttcaaggaa tcgacagttt cattacttta ctaagaagta cgattgggaa aaaagaagaa
     8821 gtgattccag tcaaggaaga aatcaaaagc gtacaaagtt acatcgatct tttgagtatg
     8881 cgatatggcg aaagaatctc ggctaaaata atgattcctg atgaactgca gtcattatct
     8941 atgccgaata tgatccttca accaattatc gaaaacgcct atcttcatgc ttttcacaat
     9001 aaaacgcaag gattcatcac ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa
     9061 gtgattgata acggcgatgg cttcgatact gaaaatacca aacaaaaaaa tgatcatttt
     9121 tcaggaatcg gcatcagcca tgtagatgag cggatacgcc taatgtatgg cgatgcatat
     9181 ggcttaacga tccaatcagt gattgggaca ggaacgactg taacgatttg tctaccgctt
     9241 cttcctaaaa atcaatcaga atatcaaaaa taaagaatag ccaagcgctg atttttttat
     9301 tttaagttaa ataaatcaat cagtattctt gactttcatc tacgcgtctc ttctactgtc
     9361 agcactggat aacagcccaa tatttgactt tctcctccga gcatcttgat ttcttggagc
     9421 gcattctcta ctaatgtcat tggacggtcc aacaacaaat cgatcacgaa aaaatattct
     9481 cccaagctag ttttcaatgg tcgggattca atcttactta agttgatttc ccgccagcca
     9541 aaagctgcca gcattttatg aagcatccct ggccgattag caggtagtgt caagatgaca
     9601 ctcattttta ctggagctga ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa
     9661 cgtgtctggt tcaactcatt atcttggata ttctcagcta agatctccaa tccaacatgc
     9721 tgtgctgttt ctaatgaagc aatcgctgct gcgtcttctt tcggatgttc tgctacatac
     9781 attgctgcag cagttgttga ttctgttgct actaaaggaa cattaggata atgcgtttct
     9841 aaaaactgtt gtgattgtgc tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt
     9901 gctggattgc ctaaaagctg ctgcttgata ggaagaacga tttcactttt aacttcgact
     9961 tctggatgtt tggaaagaag atcgattgtg tgatgaacag ctccttccaa tgaattctct
    10021 acaggaacga cagccagatc gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg
    10081 gaagcatacg cagtcaattg ttctgtagga aatagctgac aagccgcctg ataagtgaat
    10141 gaactttctg gccctagata gctgacgttc atatgctttt gatcctttct ataatggtat
    10201 cgatgatctt ttctggtgtt tgattggtcg tgttgattcg atgtgtcgca ctttcttcat
    10261 accaagaaag tcgttgtgcc aataatgctt ttatttcttc tcttgttttt ccaagagcta
    10321 aaggtcgaat agacgtttca tcttgttgga tacgatcaac tagacagtcc gcttctgcct
    10381 ctaagtaaag aacaatcggc tgtgttttta aaaaacgccg attttctgca ctttggacaa
    10441 tccctccacc agtcgcgata attttgtctt catttgacag ctttcttaaa atatctgact
    10501 ccacttttcg aaagcttttt tcaccgtaat agtgaaaata ttcggcaatc gacaattgga
    10561 tatgttcctc aatctttgta tctaaatcta catactcttt ttgtaaacat tgagctaacc
    10621 ctttaccgat tgttgttttt ccagcgccca tgaaaccaat cagcaaaatg ctagccatga
    10681 gcttctcctc ctgacacaag ttctgctaaa tcgtcaaaaa atgccggata agagattgaa
    10741 acagcttcag ctccttctag ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc
    10801 atcataccga ttcgatgatc gcctctgctg gacacttttg cgccatgcag aggtgtcggc
    10861 ccatggatga tcagaccgtc tgctgtcgct tttatctttg ctcccatttt ctgtaattct
    10921 tctgccaccg catcaattct gttggtttct tttactttta attcttctgc gtctttgatg
    10981 atcgtgattc cttctgcttg agtagccact aaagctagaa tgggcaattc atcaatcaga
    11041 cgcggaatga tttctccacc aatcgttgct ttcttcaaat gactcgtctt gacacttaaa
    11101 tcagccgatt ggttctgttc attgtgattc gtctgggtaa tcgctgcgcc catctcctcc
    11161 aacacatcta agataccagt tcttgttgga ttgaccccga cattttttaa caacagttgg
    11221 ctttccggaa caagaagtcc tgctgctaga aagaaagcag ctgatgaaat atcacctgga
    11281 acagttatct gttgaccagt taatttttgc ggtccggtca ctatgatcgt tttatcttcc
    11341 actgtgatcc ttccgccaaa ttgacgaatc atttcttccg tatggttacg ggaacgttct
    11401 ttttcaacga ttacggatgt gccttctgct tgtaatgccg caaacagcaa agcagatttg
    11461 acttgcgcac tggctacagg catattgtat cggataggtg ataaagagcg ggttcctgtc
    11521 acagtgattg gtagatactg gtcattttct tctccttgca agtcagcgcc catctcgcgt
    11581 aaaggttcca tcactcgccc cattggacgt ttagacaacg aagcatcccc gaacagagta
    11641 gtcgtaaaag gctgtccagc caaaataccc atcaataaac gtatggtcgt accggagttt
    11701 cccatgtcaa ttgattcggt tggttggaca aatgagtggc taccgcgacc atgaatcttg
    11761 atcacctctt cctcttcttc gatctcgaca cctaattgtt ggaaggcatg taaagtactc
    11821 agacaatctt ctgcgcgcaa aaaattttgt atagtcgtcg ttccttcagc aattgctcca
    11881 aacatgatgc tgcgatggga aattgattta tctgctggta tcctaacagt ccctcttaat
    11941 ccatgtattt gttgtaataa ttgcatttat ccagcctcct atttgatcca gcaatgataa
    12001 ctggtattag acataatgat tctttttgct tgtaacaaat cctgttggtt tttaaaactg
    12061 atttgcagta ttcctaaaat atcttccctg gtctcttgga tcttcaaatt aatcaaagaa
    12121 actcccgctt ttccgatcag tccagttact tctgcaataa cgccaggttc atctggcaca
    12181 tcgataaata aatcaaaaaa ggcaggaatc gcgccttgct tatggacagg caactggtct
    12241 cgactttctt ttgcccgatt gaaaaaagaa aagatttccg attggttctc agtcatgatc
    12301 cattctgcta tttgtgtcat ttcttgttgc caatctgcca atcgttccaa taatatctgc
    12361 ttattgctca acaaaatatc tgtccacatg acaggatctg aggaagcgat cctagtgata
    12421 tctcgaaagc ctcctgctgc cagttgtttg gctcgcggat actcttgatt gaattgttct
    12481 gcctgattga ctaagccagc tgccagaata tgtggaaaat gacttagcat tcctgttata
    12541 cggtcatgtt ctcttgctgt aagagttaca tattttgctt tggttccgca aaatagttct
    12601 tgcagttttt caacatcttt tttcgtcttt tccaaaggtg taaagatgta ataggcattt
    12661 tcaaatagtt tagcatctgc tgcctgtata cctgatttat gagaaccagc cattggatgt
    12721 ccaccgacaa accgaagatt tttctgctca gcggatgtca cgacttgctg cttcgtactg
    12781 ctgacatccg taatgatgac gtgatcagac aagttgattc tttcaaggac atcaagataa
    12841 gctaaggttg tacggacggg aacggctaat aagataacgt ctgcttgttc tgcacctgtt
    12901 tcaaaatcac ttgcaacgta atcaacgatc tgctgttcta ctgcttgctc acgggttttt
    12961 gactgactat cccaaccaac tactatcagt tctggattgg tctgacgaat acaaagtgcc
    13021 atagaagcac caatcagtcc aagccccacg ataaaaacat gttgtgccat cttatttgcc
    13081 ccctttttag taatgtttca agtagtcacg gtactccttc actgcagctt tcaactcaac
    13141 aagcgaatca cttgggaatt ttgacaatag ctctgtcgct accacagtgg ctacaacatt
    13201 ttccgcaacg acgctagctg caggaactgc tgtactatcc gaacgttcta cacttgcttt
    13261 gtaaggttct tttgtatcga tatcaacact ttgaagtggc ttgtacaatg taggaatggg
    13321 tttcatgacc ccgcgaatga caagtggttc gccattggtc attccgcctt cgaagccgcc
    13381 tagattattg gtacgtcgga aatagcctct ttcttcttcc cataggatct catccatcac
    13441 ttctgagcct ggtaatgaac cagaagtgaa tccgacccca aattctacgc ctttaaaggc
    13501 attgatacta accacagctt gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta
    13561 gcttcctaat ccagctggaa cgcctccaac gagcacttct acaattcctc cgatcgtatc
    13621 tccatttttc tttgtctgat cgatcaaatc ttttattctt tgctcaactg ctggatcaac
    13681 aactcgtact tctgacgctt cagaaatttc tttgatttct tgaatcgaga tatttccagg
    13741 tatttctgct tggattccac caagacaaat cacgtgtcct gctacgatga tgtcaagttc
    13801 ttccagaatt ttcttggcaa ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga
    13861 ggaccgttca agcacattcc gaagatctcg atgatggtat ttcataccgc ctaccaaatc
    13921 cgcgtgtcct ggacgcggac gtgcaactcg ccgcagtgat tggtcttttt caggcacttc
    13981 tgctacagac atgacagttg tccaattttt ccaatcttta ttttctacca ctaaagtgac
    14041 aggtgaacca attgttttgc cgtgacggat gccagacgtg atccgaacac gatctttttc
    14101 gatcttcatc cttcctcctc tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat
    14161 ttcttcttct gacagaggta atcctgctgg tagtccttca ataatggctg ttaattctgg
    14221 tccatgggat tctcccgcag tcatataacg catcttacag tcctcctaag taatctttga
    14281 atgtctctgt gggaacagga acgatttttg cttgcccaat cttttctagc aagatgatat
    14341 tcaattggct tcctcttgct ttcttatcat gggttatcgc gtggaataat tcatctggat
    14401 gacttagatc gtacgtaatc ggcagatgga atttttgaag tatcctttct aaagcagctg
    14461 tcgatccagt tggagacaag ccgatttttt ctgcatgaga agtgatggct accattccaa
    14521 tagctactcc ttctccatga ctgactaccc catatcctgc tgtattttca attgcatggc
    14581 caattgtatg accaaaattt aaaataagtc gatttccttg gtcaaagaca tcttcttcca
    14641 ctacttttcg tttgatttcc aacgtagcag taatgattgg aacagcatgt tccagaagat
    14701 ctttttcgtc tttcagctcg ttcagctgtt tccaaagcgt tgcatctgct atagctgctg
    14761 atttgatgat ttcagcgatt ccttctcgta aacgacgtac cggcaaagtt tttagaacct
    14821 caggatcaat caatacacca gctggctggc taaatgttcc gacaaggttt ttcgctttgt
    14881 ttgtattgac tgctgtcttc ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg
    14941 gaatttgaac gaaatggatc ccacgcatga acgtcgaggc cacgaatgct gctagatcac
    15001 ccaccattcc tccaccaagt gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta
    15061 aaaaatcgta taactcagct gcttgttcta aagatttact ggtctcaccc gccggtacga
    15121 ttgaaaggac tggttcaaaa ttagcttgtc tcaattggaa catgattttt tctgcatata
    15181 gtggagcaac gtttgtatca gtgatcacag ctactttttg aggtctccaa atcgtttgaa
    15241 gccatgatcc agcctgttct agtgcgcctt tttcaatatg aatatcgtat ttttggttat
    15301 caacatgtac agtaagcatt cgtttcactt ctttctttta aaaagctgga attaatcgag
    15361 tgtctgaatc ttttccattg cttctttcat gatcgcaact tctttcatca tgcgtgagta
    15421 agtcttttca ttcaacgatt ggggaccgtc tgaccaagca tttgcaggat caggatggat
    15481 ctctacgatc atgccatctg ctccgcttgc cacacccgca cgagccattg gtggtacaag
    15541 ttcccaaatt cctgtaccat gacttggatc aacaatcaca gggaaatgag ttaatttttt
    15601 catcaatggg acagcactta aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat
    15661 cccacgttcg ataaaaatga caggagattt gtcttcgact gcaatgtatt ctgcagcatt
    15721 aagccattca ttgatcgtac cggaaattcc acgtttcaat ccaacaggtt tacctgtttt
    15781 accgacagca gatagcagtt taaaattttg catattacgt gcgccaatct gtaggatatc
    15841 gctgtactca gccaccatat cgatatgccc ttcatccatc acttcagtaa tcactttcat
    15901 tccaaattca tcagccgctt gacggatgta tttcaatcct tcttcttcta aaccttggaa
    15961 agcatatgga gaagtacgtg gtttgaaggc accacctcgc agaatttttg cgcccccggc
    16021 tttggccatt cgcgcacatt ctctgatttg gtctaatcct tcgatagaac aaggaccagc
    16081 catcgtcacg aaactgccgt caccaatttt tactccgtct acatccacga ctgtattgcc
    16141 aggatggaat tctcgagaag tcaatttgta tgtgtttaaa atacgcacag cattttctac
    16201 tccttcatag cgtgtaaatg ctccttcttg taaacttcgc gtatcacctt ttagtccaat
    16261 cactactcgt tctgttcctt gatcgattga gactttgaat ccttcttttt cgatatgatc
    16321 gacgactttt tttatttgtt cttccgttgc ttcttttttc ataattacta tcattgtttt
    16381 tcccgctttc ttttgtttac ttatctatct tggctgattt tctctacttt ttcaaggtgg
    16441 ttttacatta attcctacta aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt
    16501 cctacttatg acccagataa tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca
    16561 gtgaacatct tcaacgagcc ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat
    16621 gaatttctct acgtggctgt aagtcgtgtt taaaaatctt tagccatcat agatacaaac
    16681 ctggtttgtt tgtacccacg aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat
    16741 acgtattcag ttttgtaagt tttctagatt tcataaaaaa tcgctccaaa ctgatgtaa
//
LOCUS       DAZKOW010000062        16185 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000062 DAZKOW010000000
VERSION     DAZKOW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.062"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..442
                     /gene="scm"
                     /locus_tag="ACG912_001902"
     CDS             <1..442
                     /gene="scm"
                     /locus_tag="ACG912_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0513214.1"
                     /translation="TSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASS
                     DSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSND
                     NGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(597..1583)
                     /locus_tag="ACG912_001903"
     CDS             complement(597..1583)
                     /locus_tag="ACG912_001903"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFQ0513215.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1606..2172)
                     /locus_tag="ACG912_001904"
     CDS             complement(1606..2172)
                     /locus_tag="ACG912_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFQ0513216.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2186..2602)
                     /locus_tag="ACG912_001905"
     CDS             complement(2186..2602)
                     /locus_tag="ACG912_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFQ0513217.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3059..4993)
                     /locus_tag="ACG912_001906"
     CDS             complement(3059..4993)
                     /locus_tag="ACG912_001906"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0513218.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5295..6230
                     /gene="pplA"
                     /locus_tag="ACG912_001907"
     CDS             5295..6230
                     /gene="pplA"
                     /locus_tag="ACG912_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFQ0513219.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6320..7423
                     /locus_tag="ACG912_001908"
     CDS             6320..7423
                     /locus_tag="ACG912_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0513220.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7436..8389
                     /gene="menA"
                     /locus_tag="ACG912_001909"
     CDS             7436..8389
                     /gene="menA"
                     /locus_tag="ACG912_001909"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFQ0513221.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9203..10549)
                     /gene="gor"
                     /locus_tag="ACG912_001910"
     CDS             complement(9203..10549)
                     /gene="gor"
                     /locus_tag="ACG912_001910"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFQ0513222.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10683..11936)
                     /locus_tag="ACG912_001911"
     CDS             complement(10683..11936)
                     /locus_tag="ACG912_001911"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFQ0513223.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11942..12865)
                     /locus_tag="ACG912_001912"
     CDS             complement(11942..12865)
                     /locus_tag="ACG912_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFQ0513224.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13086..15578)
                     /locus_tag="ACG912_001913"
     CDS             complement(13086..15578)
                     /locus_tag="ACG912_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFQ0513225.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15585..16052)
                     /locus_tag="ACG912_001914"
     CDS             complement(15585..16052)
                     /locus_tag="ACG912_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFQ0513226.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4754 a         3384 c         2877 g         5170 t
ORIGIN      
        1 tacatctacc acaacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac
       61 tacctctacc acaagtgata gctcagattc atctaccact acaagtgata gcacggacac
      121 gtcagcttca agtgacagta cagatacatc ctctaccaca agtgatagct cagattcatc
      181 aacttcaagc gatagtacag acacgtcgtc taccacaagc gatacacgtc atgcaagtgt
      241 ctccttctca aaaacaagtt cggataacag gacgaacttt aacttaacta ataaaacaag
      301 caatgacaat ggaaacaaat caaaaagagc acttcctaaa actggaagtc aatcgaataa
      361 ttggataaca ctcgcaggcg ttatattact tgtaatcggt ttaagaatca cttttatgag
      421 ttataagaaa tcgaaaaggt aaaaaaatat tcaagataaa aaagagcaac gtcgctaacg
      481 gacgttgctc tttatttttg ctttttatca taaatacaag aactacaatt caatttgtca
      541 aaaaacacgt agatttcctt ctgagaaatt tatttacgta tcgaaccttt ccctattcag
      601 tctttgcgat ccaaaatcag ttgggtaagg gaaaagagat tctcttttgt tcgaagagag
      661 gtggcaggta atttctttat ttccttcaaa gccttttctg tgtaagaggc cgctaacttc
      721 ctagcttctt ccacgcctcc tgtatgttca acgattttgc gtacggcgtc gacatcttct
      781 tctgtcattg ccgctttctt ctctaaataa ggtagtagct ttggtttgtt tttttgcaaa
      841 gagtagatca acggtaaaga atagacccct tgacgcatgt cttctagtac aggtttgccg
      901 atttcctggc tctcttttgt ataatctaaa atatcatcga taatctgaaa ggccatcccg
      961 atagaaagtc cgatatctcc tgcttttttt gcgaagcgct cgcttgttcc actttcaaaa
     1021 gcgccaacag aacaacttaa ttggaacagt tcagctgtct tacctgaaat attttccaaa
     1081 tattctttta cagaaagttc ataattatag cgatcatcca tctgccctaa ttcgccattc
     1141 aatacttttt ccatactgcg agaattcaat tggagacttt tcaaggaagt ggagtaatca
     1201 gacaatagtt taaaacagca gacaaataaa tagtctcctg catagacaga aacgacatta
     1261 ccaaactgac tacggatatt aggctgacca cgtcgcagat ccgcttcatc aacgatatca
     1321 tcatggatca gtgtggccgt atggagcatt tcgatagaag ctgccaaagc gatggctttg
     1381 tctcgatctt gctctggtcc aaattgtgag aacaatagct gataggcagg acgcaacagc
     1441 tttccgccgg catggatcgt ttctttgatt gcttgttcga caggtttgtt tttcaggcgg
     1501 acagtttctt ccatccgttt tagtgtttct gtcaattctt tacttaaagc aggatagttt
     1561 tcccacattg gatgtagctt cataaaaaaa ctccttgtgt gacgattatt ttttggtaaa
     1621 cattgggctc catttttgtt tggttccctg ctcataatct ttttggaatc gttgaagtgt
     1681 ctgcacatgt cgtaaaagat aattcgctgc gatcccgatt gcaatccctg ataaaatccc
     1741 taaaactgaa aggactggca agtacaacat gactgtccat gattgtgcaa tgaaagaagc
     1801 catcaaaagc tgtccgacat tatgcatgaa accgccggcc gcactaattc cgatgatact
     1861 gacacgtttt ggtcctaatt gcttgaccaa taacattcca aaataactaa gcaaagcacc
     1921 actcatacta taaaggaatg tggaaatcgt tccgcctagc aaagtagtca ggaatagtcg
     1981 tagccaaagt agtaagaagc tgtctcgttt tttcatggta aaaatggcaa cgatcgtgat
     2041 tagattggct aagcctagtt ttgcaccagg cgcaaaagaa aaaggataag ggatcatatt
     2101 ttccaataaa ccaatgatca ctccttgtgc aacaagtagc gaaatataaa tcattttttg
     2161 taatttactc atggtgtgtg aaggtctaat aaatcagact gtcttcgtca cctccatctg
     2221 ttgaacggac ttcgatcact aatttatgtg gcaaacagac gatggtttcg ccatttttcg
     2281 ttgcccagcc tcgacgtacg cagatctggt catcgcaatc tgcttctttt atccgtactc
     2341 gatcgccatc tatctcgatc aagttataat ctccgtgttc atctttgtaa gtatatgtgt
     2401 tgctctcttc tccagccatc aaagggaatg ttttgatttc tgtgccatcc acacgcagta
     2461 ccgcttcttt ttctgctgtt gcggtgtttt gctgaagacc aaaaatcatg actggtaaaa
     2521 aagaaagaac agtcaaaagc aaaataataa aaacatccca aggtttgagc agactttttt
     2581 cagcaaattc tttaaggctc atttttgttc tcctttttcc agattaactc acccaatttt
     2641 agcataaaaa caagaggttg tgacacctgt gtcttgcttt gagtaataag aacgaagaat
     2701 agaaaacagc tttccatgtt ttctaattct ttgggcttat taccgagaag cagcaggtgg
     2761 aacaccgttt ataacaagag gttgtgacag aagtgggcaa cttcaagaaa taagaaggaa
     2821 tttacgaaaa ttacttttca agtttttgtg aaatttcggc ttatttttcg aagaagctac
     2881 ttctggaaca ccgtttataa caagaggttg taacagaggt tgtgacacct gtgtcttgct
     2941 catggaggct tatctcgggc atcttattcc tttaatttta cataaaaagg aggctgtgac
     3001 ggtttgtctc agcccctttc tattgaaatc ttgcattcaa ataacgttct ttatctattt
     3061 atttcttgcc gtactgggaa cttggtctac cgtaccacca attacctagt gtacgttgta
     3121 gccatggaat tacccagcga tccaacccaa tcgcacgtcc ggaaccgttc atcaatgcga
     3181 aagcaacaaa aatgaaccaa atgtttaccc agtaaaacat gcctgataag cagaacgtga
     3241 tcaccaatac aatcgttgtc gcactgcata gccatgtgaa taatccagca atcaatgcta
     3301 atgccaaggc aatttcaaag aataccatga atttttggaa gaacattgct acatcattgt
     3361 ttggcatgat cacttgcatg atctttgtga accagtctgg tgacttctca atcacagcca
     3421 ttggttcttc accgtatgca taactcaaac caaaatgtgc agcttgagtc gctgcttctc
     3481 cggcacttgc ccctgctccg ctggcagctg tggttgcatc accagcagca gaagcaccac
     3541 tggcaacatc tgttacattg tattcccatg tccaagggaa cacactgttt ccagtgaacc
     3601 atgatccttc accaaaccaa gtacttggtt tgaacacttc accattccca atgatttttt
     3661 tggaagcttc aacgaaccaa actaaaccat agaaaatacg aagtggcaca ctccataaga
     3721 cattgccgta acgagaagaa tgtcctcgag tcacgttgcg atcatctttg atatggaaaa
     3781 actcatgcat taaatattgg aacatataat aaccagaacg aatatcaaag aagtatttca
     3841 aattcacgat atgtttcatt acgatagcta aaaatccgct taaatggatc ttatcaaata
     3901 ggttagctac cccccatttg gcaccaatag aaaccataaa tccttgatag tttcctttaa
     3961 atttatgctt ttctccgcct ttgatcgaag ccacgatatt cgctgcggct gtgtgtcctg
     4021 tttgttcagc agcttgaacg atctgcggtg tcggtgttcc tgggaattct tcataaaaga
     4081 ctaaatcacc aatgatatag atgtttttat cttcgtagcc ttttgcctgc atatattcat
     4141 tggcgatcaa ccgctgtccg cgtgctgatt ctaatccaaa gtcagcagcg tcagaagttc
     4201 ctttgacacc agctgtccaa atcagtgtgt gagtcggaat gttgcgtccg tctttcaatt
     4261 tgatatgatc agcagccact tctacgattg gtgcattcaa taataattcc acgttgtgct
     4321 tacgcaaata gcgttcagct tttgcagcat catttcgatc caacatattc aagatcgtcg
     4381 gcattgcctc tacgaccatc agtgtgattt cgtttggatc taatttataa tctttggcta
     4441 ggcgattttt ccagtcgatc agttccccaa tcatttcaat accggtaaat ccagaaccac
     4501 agacaacgaa agtcagcatg gctttacgca gttcatcatc tggttccaat gcagcttttg
     4561 ctactgtctt ctcaatatgt tcccgaatcc ttacagaatc ttcaaatgac caaagggtga
     4621 atccgtgtgc tttgacacca ggtgtaccaa agtcattcgg ctctccgccc attccaagga
     4681 tcagctgatc aaaaggatag gaacctaatt tggtcttgac gatttttcgt tctttatcga
     4741 taccgattac agtgtctgtg acaagttgta cattcttttg acgagcaaac aaatgctgca
     4801 gatcgtattg gattgcttct ggttctacac gtccgccagc tacttcatgc aactcggtca
     4861 tcatagtatg gtacgaatga cgatcgatca gagtaattgc tacatcttct tttttcagtt
     4921 tctttgccaa gtattttgta gctgaaacac cggcatatcc tgctcctaca acgacaatat
     4981 tttgctttgc cattgatcat ccgctcctta tagactatct atataatgaa tatatgataa
     5041 ttcttttagt ttgatactta ttacacaatc tccatatatt atatagatgt tctgttcttt
     5101 tgtctaactc cttttggatt tttttcaaat tttaaaataa agcgtttttt cacgaaattt
     5161 gctgaaagaa aacgtttgaa atattacatg ggttatttat caaaaatagt tgacttatag
     5221 gctaaaaaga ttagaatgta taatgaatca caaagaaaaa ttgtggacta gaaatggaat
     5281 ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg atttgccgta ttagcatttt
     5341 cagctctagt gttaggcgct tgcggcgcag atgacaacgg aagctcaaac tcttcttctg
     5401 aatcatctac agcacaatct tcgactgcaa aaagcacaac agaatcatct gcaaaagttg
     5461 tagctggcgg agatttgcaa gacggcacat ataaattaga agaaaaaaat tattcaaatg
     5521 gttaccgtgc agtctttgaa atggttgtaa aagacggcaa aatcactgaa tcaaaatatg
     5581 ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc agaatacaac aaaaacatgg
     5641 aagcaaaatc tggcacaaac ccagaaaaat acattcctga gttgaatgac caattcttaa
     5701 aagcacaaag cgcaagcggt gtggaagtcg taactggagc tactcactca tctgaaagct
     5761 tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc tggtaacaca gatacaatcg
     5821 aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa gcttgaagaa aagaactact
     5881 ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg cggcaaagta actgaatcaa
     5941 actttgatta tatcgacaaa gacggtaaat caaaacaaga tgatacagaa tataatgaaa
     6001 atatgaaagc aaaaagcggt acagaaccaa aaacttacat ccctactctg aacgacgaat
     6061 tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa tgtagaagtt gttacaggtg
     6121 ctacacacag ctcacattca ttcatcatgt acgcacaaca attagtgaat gcagctgaaa
     6181 aaggcgacac tcaaacaatc gaagtagata atatcgtaac agaaaaataa tcacataatc
     6241 atactaataa aagttggagg gtgggacgat agccagatta ggcagtcgtc tcacctttca
     6301 aaatgaagag aggaaaaaga tgcggaaaaa actactattt tccttaatca cactgggcat
     6361 ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat aaaacaacca ctgaaagttc
     6421 agcagctacc aagatattga aagatccata ttctgatgaa cagttcttgt tagggactta
     6481 cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca ttgaaacctg ccttcgatcg
     6541 agtaaaagaa cttggtgata aaatcacgat caatcaaaaa ggttctgaga tcgatgaagt
     6601 gaatgagcaa gctgggatca aaccagtaaa agtttctgat gacgtctaca cgttagtcaa
     6661 gcgggcctac gaatatagcc aagattctca aggcggattc gatatggcga ttggtgcaat
     6721 cacacaatta tggcgaatcg gttttgatga cgccagaaaa ccttcccaag aagaaatcga
     6781 tcaggcattg aaattagtgg attatcataa gatcgaacta aatgacaaag aaaaaactgt
     6841 ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca atcgccaaag gttacattac
     6901 ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc actgccatcg ttgacttggg
     6961 cggtaatgtc tatgtcttag gccacagccc aagaggcgaa aaccaagact ggacagtcgg
     7021 catccaagat ccgaacatgg ctcgcggatc tgttttaggt tctattaaag aacggaacaa
     7081 aacattagtt acctctggaa tttacgagcg ttatttagaa gtagatggca aaaaatacca
     7141 tcatttgttt gatccaaaaa cgggttatcc gttcgacaat gatatcgcaa gtgtcacgat
     7201 cataacagat aaatcaattg atggtgacgg gttgtctaca gcagtctttt caatgggtgt
     7261 gaaaaaagga ctggaatacg tcgaaagcga attgaataac gggacggaag cgatcttcgt
     7321 gacaaaagac gataaagtat atgtgaccga tccaatcaaa gacaccttca aattatcgga
     7381 ggactcccat tacacgatgg gtgaccgttc tgaactaaaa taaggagtac gaaccatgac
     7441 actaaaaatc tttttagaag tagttgaaat acgaacaaag gtggctagtg tatttccttt
     7501 tgcggtaggg gtattattct ctatcgcttt ttttcatgag atacattggc taaacacttt
     7561 tttatttttt attggaatgc tagtttttga tttagcgact accgctatca ataattacat
     7621 ggattatgaa aaggctcatt ctgaagtata taagtatgaa gaaaatgtca ttggccgcag
     7681 aaatctatct cctgtattga ttcgaaacat gattttagct atgattgcat ttgtttttgt
     7741 tatcggcgtt attctaacga tcaatacggg ttggctttta cttttgatgg gatttatctg
     7801 ttgttttatt ggagtattct atacattcgg accgattcct ttatctcgaa tgcctttagg
     7861 ggaaattttc agtggggtga ctatgggcct tggaattttt gccatggtcg tttatgtcaa
     7921 tacattcggt tcagatgtct atgatctgac agtcaacttt tcatctggac aatttgcttt
     7981 ggtaggaaat ctttgggcta tagcagcaat cgtattagct tccttaccct tggtgtttac
     8041 gattgccaat atcatgttgg ctaataattt gcgggatctt gaccgtgata ttgaaaatca
     8101 tcgctataca ctagtttttt atatcggacg tcctgcggga acagtcttat tccagctatt
     8161 gatgtatgct tgctatttcg tgattttaat ggggttgttg agcggtatct atgaatggcc
     8221 gatcttggtc accttcttga ccttgccaat tgtgaagaag catttggatc aattcaagca
     8281 aacgttaccc cagcctatca gtttcagcta ttccatcaaa aatatggtct tattcaatgc
     8341 cagttatgta ttggggctat tactaacaat cattatgcaa gccatttaac agcatgaaga
     8401 ggttgtgaaa aaactgccct gcatcaagta ataagaacag ccaaataaaa atagctcctt
     8461 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatca
     8521 ggaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggctt
     8581 tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat
     8641 cagaggttgt aaaatcagtg ttttgtcctg agtattagag aaacaaatgg aaaaatagtt
     8701 cctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa cactatttgt
     8761 tcggaaaaag tgactaacta tgacacaatt tttttcacag tccctttttt cacttgattt
     8821 gattcgtacc agcgaacttg acgagaactt tcttggtttg gtaactatat ggtattcttt
     8881 taacatcctc gtttgcttct tctaatttat ttatacaaag tcacactttt ttaaacatct
     8941 gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga acttaagagt
     9001 gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa atctccaact
     9061 tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa ctaaaaaaca
     9121 aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc aataaaaagc
     9181 tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct cctgttggat
     9241 ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata gccacggcaa
     9301 aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg attctttctt
     9361 cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt aatgcaaagt
     9421 acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat ttttccaatg
     9481 cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg actgtcggaa
     9541 caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct gataaacgtc
     9601 taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca ccaacagcaa
     9661 aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct tttttgtcca
     9721 attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt ccaccggcaa
     9781 aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta tattcgtcat
     9841 tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca gcttcttggt
     9901 acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt cgttcttttc
     9961 gatatgccca catgacttca ctacctaaac cacgaaatac acctgaaagt tcagccgcaa
    10021 tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca aaaaatccat
    10081 ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg cctccagtag
    10141 cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt tgactatcga
    10201 taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct ctttgataag
    10261 caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg aaatcaacat
    10321 tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg
    10381 tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat gttccgccca
    10441 attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg tttgctgaag
    10501 caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt ttgttcctcc
    10561 ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag aaaacaagtg
    10621 taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata tcagcttttt
    10681 ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac tggcatagtt
    10741 aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt atgcataact
    10801 tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc atctcctaca
    10861 gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata agataatact
    10921 tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg ttcgttttca
    10981 gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt ccataactcg
    11041 tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact gtctacagcc
    11101 gctttataga catttgacac cgtagataca taatggatcg atttcattct tccttcgatc
    11161 ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat tgacatatcc
    11221 actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact gtttcgttct
    11281 tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga acatccgcca
    11341 cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga ataagagata
    11401 cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg aatctctgcc
    11461 acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc ttctttccag
    11521 aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat cggcagacct
    11581 ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac tgcagaaata
    11641 ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc ttcatgggtg
    11701 accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc aactccttca
    11761 gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc attcccgcca
    11821 atatatactg catctgctcc ataatggatc gcggttttta acttttccag tgttcctgct
    11881 ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt catcatactc
    11941 cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca cggttttcag
    12001 gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc cagctacctt
    12061 ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg
    12121 gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt tcattcaatt
    12181 cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta tcttcataaa
    12241 tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt tcacgatctt
    12301 tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt ttggattgat
    12361 ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt tcttccatcg
    12421 ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg ccttttttcg
    12481 cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa atatacggta
    12541 tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca actgtgatca
    12601 catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc attttttcag
    12661 gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt gctaattcta
    12721 ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca aattgctctt
    12781 caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt tcaatagttt
    12841 ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata atagtagcat
    12901 agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa aattatttca
    12961 caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga ttgtgacaaa
    13021 aatcttgtca caacctctta atcaagtctt actattataa aaattcagct acacaggcca
    13081 agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa tgtgatctta
    13141 ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc
    13201 tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg tgcaccatat
    13261 tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat tttcacttga
    13321 atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac gattgtatgg
    13381 atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt caagaattca
    13441 ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt gtgatcttta
    13501 gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt cgctccgata
    13561 tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt agaatctgtt
    13621 aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc
    13681 acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt caactgtcca
    13741 ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt actgtatttt
    13801 tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa catgacttct
    13861 gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa catgaatgaa
    13921 ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact
    13981 gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc cagttctaac
    14041 aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca ctccgataca
    14101 actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg ttttgcttca
    14161 attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg taattgagca
    14221 gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag ttcatcgatt
    14281 tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg gactttcgct
    14341 gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt gatataacga
    14401 acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc atggtgctct
    14461 tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt tggctcatct
    14521 acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata tttttgatat
    14581 tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc cggtttcaag
    14641 atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt atgcaattca
    14701 tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt tttcattcga
    14761 tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc aagcatcatc
    14821 aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg agctaaccct
    14881 tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt attctttgtc
    14941 cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac aactgggtcc
    15001 agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt
    15061 ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt cattcctttt
    15121 ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc ttttgaaagg
    15181 ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt taataagctc
    15241 atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc gctgctagct
    15301 aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt agggtaagaa
    15361 cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt ttcattcaaa
    15421 tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa ggctagtaaa
    15481 acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg tgcaatttct
    15541 aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt cccatcttca
    15601 taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc ttcatattca
    15661 ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc tttttttgta
    15721 attatttggt cttcataaag tttttgaata atagcaaaag catttttctc actgattgta
    15781 tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat ccttactttt
    15841 gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc acgttggatg
    15901 gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc
    15961 atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt tatatatgat
    16021 tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga ctaatattga
    16081 ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt taaataaatc
    16141 ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatc
//
LOCUS       DAZKOW010000063        16157 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000063 DAZKOW010000000
VERSION     DAZKOW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.063"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            226..894
                     /locus_tag="ACG912_001915"
     CDS             226..894
                     /locus_tag="ACG912_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFQ0513227.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1414)
                     /locus_tag="ACG912_001916"
     CDS             complement(1094..1414)
                     /locus_tag="ACG912_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513228.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1451..2833)
                     /locus_tag="ACG912_001917"
     CDS             complement(1451..2833)
                     /locus_tag="ACG912_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0513229.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="ACG912_001918"
     CDS             complement(2989..4176)
                     /locus_tag="ACG912_001918"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFQ0513230.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="ACG912_001919"
     CDS             complement(4183..5214)
                     /locus_tag="ACG912_001919"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFQ0513231.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5230..6684)
                     /locus_tag="ACG912_001920"
     CDS             complement(5230..6684)
                     /locus_tag="ACG912_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFQ0513232.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            6859..7764
                     /locus_tag="ACG912_001921"
     CDS             6859..7764
                     /locus_tag="ACG912_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFQ0513233.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="ACG912_001922"
     CDS             7855..8532
                     /locus_tag="ACG912_001922"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0513234.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="ACG912_001923"
     CDS             8529..8699
                     /locus_tag="ACG912_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513235.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="ACG912_001924"
     CDS             8813..9709
                     /locus_tag="ACG912_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0513236.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="ACG912_001925"
     CDS             complement(9764..11134)
                     /locus_tag="ACG912_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0513237.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="ACG912_001926"
     CDS             11372..12118
                     /locus_tag="ACG912_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513238.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="ACG912_001927"
     CDS             12115..12954
                     /locus_tag="ACG912_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFQ0513239.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13905
                     /locus_tag="ACG912_001928"
     CDS             12955..13905
                     /locus_tag="ACG912_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFQ0513240.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14046..14798
                     /locus_tag="ACG912_001929"
     CDS             14046..14798
                     /locus_tag="ACG912_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513241.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14817..15968
                     /gene="queG"
                     /locus_tag="ACG912_001930"
     CDS             14817..15968
                     /gene="queG"
                     /locus_tag="ACG912_001930"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFQ0513242.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5244 a         3011 c         3243 g         4659 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgctag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gatctactgc atcgtgaagt tcatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaaatg cagaagcata tgtggaaaaa
    13501 ctaaaagaat tagatacaca agcgaaggaa agttttgctt ctatcgaaga gaacaaaaaa
    13561 ttattagtaa caagtgaagg ggcattcaag tatttttcac gagcatatga tttgccagca
    13621 gcttatatat gggagatcaa tacagaaagt caaggtacgc ctgatcaaat gaaagcgatc
    13681 attgatcaga taagagcaaa agaagtaccg gttttattcg tggaaaccag tgtagactca
    13741 agaagcatgg aacgggtagc aaaagaaacg ggtttgaaaa tctatgataa actgttcact
    13801 gattccatag caaaagaagg agaacaagga gattcttact atcagatgat gaaatggaat
    13861 attgaaacga tccatgaagg tttgtcacaa acaaaagaaa gctgaaaagt gcaaggtcta
    13921 gcttgaggag cagaccttgc attttttata agagatatga aaaaaaatta tgctgttttt
    13981 cgtcgtacag ataccttttt tgaagaaatt ccgttatagt tagagtaagg aaggaaggtg
    14041 tggctatgaa ttttacacgt aaagataaat atacgattaa agaagaagtc tttcgattga
    14101 tcagccagca ggaaaatacc gacaaggatc aagaccttca atttcttgaa gcagaattag
    14161 ctaaattatt catagcaaat caaaatgaga caacagaacc agatgaagag ctgtacatag
    14221 gcaaatggtt gtttgatgaa tttcagcctt tagatgaaaa taccggtatc ggttattttg
    14281 tgttcctttc tccagtcggc ttcacacctg gcgtattagt taaacaaaac gacgaaataa
    14341 ccgtcaaagc atggcctgca ttattttcag tcatcaaagc agaaggagta atcaaaaaga
    14401 tccagtcggc ctattataaa agtcaattta ttgccaacag caaattaatg aatatgtttt
    14461 ataatcgtct agtagaaatt gctaggcctg ggaaaatccc gcaaacattc acgtacacac
    14521 agcaaaaaaa tctctatgaa gaagtattgt attcccgcga tctgtctgag ttttcgccat
    14581 gggaagaggt aaggctccat gaactgttaa gtacaaaacc agtttcattg aattttttta
    14641 atgcattaga taacaaaaga aaattaattt caacaaatta ttgggaagta tttgctgcaa
    14701 gaaaaaaatg gatggaagaa aagaagattg ccgatcagca gatccagttt gtccagctga
    14761 aaaaagaaaa actatattat gggttataca gccgttaaca gcaagcgagg aagaccatga
    14821 cattaaaaga agaaatcata caagaaagca aacgattagg aattgacaaa atcgggttca
    14881 ccacggcgga accttttgat tcattaaaag aatcgttgga agaacaaaaa gcagctggcc
    14941 atacttctgg ctttgaacat ccaaatatcg atgagcgtct ttatccagaa atgacatttg
    15001 atcagccgaa atcgatcatt tccattgcac ttgcgtatcc tactaggatc catgaagaag
    15061 tgcctaggga tgaaaaacgc gggcagtttg ctcgtgcttc ttgggggatc gattaccatg
    15121 atatactcag agaccgtttg aaccgattga tcgaattcat ccaaacaaga gcagaaaaat
    15181 ggcaaaaaga agaggaatgg cgtctagctc cgcaagtaga tactggagaa ctgatcgatg
    15241 tcgctgtagc acagcgagct ggtttaggat ttattggacg aaacggattg ttgatcactg
    15301 aagagttcgg ttcatttgtc tatcttggtg agatcgttac taatattgta tttgagcctg
    15361 ataaaccagg agaatttggt tgcggtgact gtatgcgctg cgtgactgct tgtccgacaa
    15421 aagcattgct tggtgacgga agaatgaatg cccagcgatg tttgtcttat caaactcaga
    15481 caaaagggat gatgccggaa gaatatcgta aaaaaatgcg aaatgtcatt tatggatgtg
    15541 atatctgcca gttagtctgc ccttacaatc aaggaaaaga ttttcatttt catgaagaga
    15601 tggaaccaaa agtagacgaa atatatccaa aattaaagcc aatgctttcg atgtctaata
    15661 aagacttcaa acagcaattt gggcatttag ctggttcatg gcgtgggaag aaacctttgc
    15721 agagaaacgc attgattgct ttagctaatc taggtgatcg aacggcattg cctgagattt
    15781 cagaatgttt aacagatgtc cgccctgtca tcagaggaac agcagcttgg gctattggac
    15841 gtctaggaac aaaagaaccg gaaaaatggc ttgatgtatt agatgaaata ttagctaaag
    15901 aaacagaaga agtcgttata aacgaattgg aacatgccca gaaattattg cggagaaagt
    15961 taaaataaaa aaatagttaa cattattcaa agtttttgtc cagtaattgt tgtaacaatt
    16021 actggttttt ttgtattttt tacatttttt taagagaatt tgataaaaat caaaaatttt
    16081 tttgcttttt tttatggatt attgcagtga atgttacaga atctgcgttt ttcattcaaa
    16141 aataaaaaaa catagaa
//
LOCUS       DAZKOW010000064        15747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000064 DAZKOW010000000
VERSION     DAZKOW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.064"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(28..726)
                     /locus_tag="ACG912_001931"
     CDS             complement(28..726)
                     /locus_tag="ACG912_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFQ0513243.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="ACG912_001932"
     CDS             complement(719..2071)
                     /locus_tag="ACG912_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFQ0513244.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="ACG912_001933"
     CDS             complement(2294..3694)
                     /locus_tag="ACG912_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFQ0513245.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="ACG912_001934"
     CDS             3855..4592
                     /locus_tag="ACG912_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFQ0513246.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="ACG912_001935"
     CDS             4717..5196
                     /locus_tag="ACG912_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFQ0513247.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="ACG912_001936"
     CDS             5193..5960
                     /locus_tag="ACG912_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFQ0513248.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="ACG912_001937"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="ACG912_001937"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0513249.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="ACG912_001938"
     CDS             complement(6863..7465)
                     /locus_tag="ACG912_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFQ0513250.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..7801
                     /locus_tag="ACG912_001939"
     CDS             7571..7801
                     /locus_tag="ACG912_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513251.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7792..8040
                     /locus_tag="ACG912_001940"
     CDS             7792..8040
                     /locus_tag="ACG912_001940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0513252.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8146..8730
                     /locus_tag="ACG912_001941"
     CDS             8146..8730
                     /locus_tag="ACG912_001941"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFQ0513253.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="ACG912_001942"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="ACG912_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFQ0513254.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="ACG912_001943"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="ACG912_001943"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFQ0513255.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="ACG912_001944"
     CDS             10631..10810
                     /locus_tag="ACG912_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513256.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="ACG912_001945"
     CDS             10832..11857
                     /locus_tag="ACG912_001945"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFQ0513257.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /gene="glyA"
                     /locus_tag="ACG912_001946"
     CDS             12032..13276
                     /gene="glyA"
                     /locus_tag="ACG912_001946"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFQ0513258.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="ACG912_001947"
     CDS             13429..14376
                     /locus_tag="ACG912_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFQ0513259.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="ACG912_001948"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="ACG912_001948"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFQ0513260.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="ACG912_001949"
     CDS             complement(15278..15520)
                     /locus_tag="ACG912_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513261.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="ACG912_001950"
     tRNA            15641..15713
                     /locus_tag="ACG912_001950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
BASE COUNT         5350 a         2823 c         3196 g         4378 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg attttta
//
LOCUS       DAZKOW010000065        15423 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000065 DAZKOW010000000
VERSION     DAZKOW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.065"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1016..1357)
                     /locus_tag="ACG912_001951"
     CDS             complement(1016..1357)
                     /locus_tag="ACG912_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFQ0513262.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1357..1587)
                     /locus_tag="ACG912_001952"
     CDS             complement(1357..1587)
                     /locus_tag="ACG912_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0513263.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1874..2212
                     /locus_tag="ACG912_001953"
     CDS             1874..2212
                     /locus_tag="ACG912_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513264.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(2417..2979)
                     /locus_tag="ACG912_001954"
                     /pseudo
     CDS             complement(2417..2979)
                     /locus_tag="ACG912_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(3711..4292)
                     /locus_tag="ACG912_001955"
     CDS             complement(3711..4292)
                     /locus_tag="ACG912_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0513265.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(4475..5101)
                     /locus_tag="ACG912_001956"
     CDS             complement(4475..5101)
                     /locus_tag="ACG912_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFQ0513266.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(5123..6454)
                     /locus_tag="ACG912_001957"
     CDS             complement(5123..6454)
                     /locus_tag="ACG912_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFQ0513267.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(6609..7010)
                     /locus_tag="ACG912_001958"
     CDS             complement(6609..7010)
                     /locus_tag="ACG912_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFQ0513268.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(7096..7302)
                     /locus_tag="ACG912_001959"
     CDS             complement(7096..7302)
                     /locus_tag="ACG912_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513269.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(7397..8899)
                     /locus_tag="ACG912_001960"
     CDS             complement(7397..8899)
                     /locus_tag="ACG912_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFQ0513270.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG912_001961"
     CDS             complement(8917..9372)
                     /gene="argR"
                     /locus_tag="ACG912_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0513271.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(9470..10087)
                     /locus_tag="ACG912_001962"
     CDS             complement(9470..10087)
                     /locus_tag="ACG912_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFQ0513272.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            10308..11681
                     /locus_tag="ACG912_001963"
     CDS             10308..11681
                     /locus_tag="ACG912_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0513273.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11756..12868
                     /locus_tag="ACG912_001964"
     CDS             11756..12868
                     /locus_tag="ACG912_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0513274.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12940..14577)
                     /locus_tag="ACG912_001965"
     CDS             complement(12940..14577)
                     /locus_tag="ACG912_001965"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFQ0513275.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14745..15353)
                     /locus_tag="ACG912_001966"
     CDS             complement(14745..15353)
                     /locus_tag="ACG912_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0513276.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4267 a         3166 c         2689 g         5301 t
ORIGIN      
        1 aaccaacagc aaaaaataca atgaaaaaca aacttggaac taatgtatca ttaataaatg
       61 ttgaaaaatt cagcaactca ttactgtatg attttagcag cccaaccaac atatacccta
      121 taaaagctaa taagatggtt aggaatcctt tagaattttt actcattcat agacacttcc
      181 ttaaataact tttattatag aaggtattgt aaataataat attgacaaaa agtgactcaa
      241 aataaaaaaa accttacatt atactggaat gaactgtatt gatagtgtac ctaaaagctc
      301 tactgacata agatcgtgta atcggtaaag taattttatt aattatacgg gatgtaatca
      361 attagagaat aaactaatca taaaaggtac ttttacattc ataattgtcg ctttgagatt
      421 ctactttggt tcactatctg acccaattgc acattttcga tcattacaag tattggtgtc
      481 gttagaacaa ctaaataaaa actagcggta gatttttcag aatctatgct aatttctgta
      541 tggatgttcc agtgatggca aatttctctt ttcattgttt ttcttgtcgc atgatatgcc
      601 tttttgtgtt attcagttaa actatagaaa ttctttccta gacagatctt gatcccaaat
      661 tttacgtttt tgagcatcga tatagtttaa aatgacatta ctcatatcta aagagtttag
      721 attttttagg actaaaactg tcagttgttc tttttcttct ttctgttaaa gtattgtcgc
      781 gacttttcgt atacgttaag atatgtcgtg aaaaaacaag ttttgtgaca caaaaaaacg
      841 aatgacttga ggaaatcacc tcaatcattc gtttaaaatg ctatatattc tttagtttaa
      901 gatacggaaa ttaatgccat cgaccacact agtagaatga acgacattta cttggccttt
      961 ttttaatttc gttatgttta gcatataaaa atattataga gtttacttac aatgcttaaa
     1021 acatatactg tataatctga tcaattttag ccatgtcttg caatggtaag ttttctattt
     1081 ttttgagttt tcgttcctta aaatcaagag attttaattg tgtaataatt acttgtcctt
     1141 ttatatctag ctcttcaggt aatgagtaac gtgtcggtaa attctttata gtggaagtaa
     1201 ttggacatat gaccgaaaac attgttttac ggttaaattc ataacgagag acgactagtc
     1261 ctggtcgtct tttttgaatt tcttttccta cggagggatc aaaatcaatc catacaatat
     1321 cacctttttt aggaatataa attctttctt ctgacactat aaaatttctc tcccttcagg
     1381 agttaaatct tcccattcgt ctttctcata atactcggcc tcttccccac cactaaatgg
     1441 atcttcaatt tttggaacta acgtaattgt tccatcatca gaatacacaa cgatatattc
     1501 ttggttttct gaaggtttct ttccgttatc agaaggtaaa gttaccacta cagaacttcc
     1561 ttgtaatcta gattttgtaa caagcattat catcactccc ttaataaaag tatactatat
     1621 aacactaagt aacgcaaaat tcccctttgt aaactatcta gaaaattgtt ttgtttaaag
     1681 tatatcttat tggatataaa tagaaataaa ttgtagccag ttcatttttt ctttaactaa
     1741 aaaaataatc caagaaacat tgaacctatg gatccaaaag ctacccaaat actatagcta
     1801 accccccgct accttcaatt ttgcagataa aacgctttgg gagaaaattt caaagacaac
     1861 aggccatcct atcatgcccg attataaaca aaaagctggc tattcctttt gctctgacaa
     1921 atttcaattg ggaaagttca cgaaactagc aggtgaagaa gtttcaactg ttcggatcaa
     1981 agaatgcccg cttcaaatag aaacattggt tcaacgaatt tcaactagac aagactttgc
     2041 tattgtagaa cgtgagatcg tagctatttt tgtagaagaa gggattttac atgatccaac
     2101 tcatatcaat gtggataaat ggcaacctct tatctataaa ttcagagaat atacaactgc
     2161 caaccagtcg ttaactttga attttagatt ccaagaattc aaagagtcat aatgataaac
     2221 atcgttaggt ataaataaaa aagactcgca gcacgagtaa aaaacaaata gaagctatac
     2281 attagaaagg tcgactgtga ccctatagat gcagtatatg cagcttatga gcatttcttc
     2341 tgttctcttt gtttttggga tctgtgctga agtctttttc gttttttgcc gctctttctt
     2401 tgaaaaatcg attatgttat accttctcca ttactaattc aggcatctgc tgagtaatga
     2461 tttttcgcgc aaaagaaata tctgttaccc aaggttcgat ttgttcttca gtcaagacaa
     2521 gcggcattcg gtcatggatt ggtgcgactg tgcggttagg ttcagtagtc attatgattg
     2581 attctgccat tttttcatcc tgatggctac gataaaaacc gccaatatac actatttttt
     2641 ccttatttga aaacataaat ttgtcttttt cttcattcca ctcattggga aaacacaacg
     2701 attttgcaaa aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt
     2761 aatcatcaat tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag
     2821 gatgttttct tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc
     2881 tgctaattct gctgcctgct tcggttgttt cgtctgggct tctcgataat aattctgaag
     2941 gagctctgac tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt
     3001 tttctatcat acactgcaac aagaagttcg caccaacaaa acgctttcaa tttttgtaga
     3061 actaaaaaag ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc
     3121 tcggtcacaa gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg
     3181 tgactcggag tgaaacggca ggcttacgac ctctgggtaa acggtgttca aaagccagtt
     3241 tctcggtatt aagccaaatt tatgcaaaaa tgtagaaagc tattttcaca aaatttttct
     3301 taatactcga aactaagcga ctttttcaca acctcttcct taaacggtgt tcaaaagctt
     3361 gcatctgcgg taataagccc aaccaaacga aaaatatgag cagctatttt tatttggctg
     3421 ttcttattac ttgatgcagg gcagcttttt cacaacctct ccactaaacg gtgttcaatc
     3481 agctaacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
     3541 tttctctaat actcaggtca aagcgctgat ttcacaacct ctaatacacg gtgttccaga
     3601 agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca attttcgtaa
     3661 attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta ttaaagaacc
     3721 ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag tagtgataga
     3781 tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa cgctttggct
     3841 tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat attttgtttc
     3901 gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc ttcatccact
     3961 gtactcaact caataatcaa attacctaca ggacagccat gataagtgat ctgtgtctca
     4021 tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg ttttccagca
     4081 tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg gatcaccgct
     4141 agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc tgtatcagaa
     4201 gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat aagatctttg
     4261 gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca tttctcatag
     4321 tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa aagactgtgc
     4381 caaattatct gacacagccc tcactataaa actgttctca tatagctaga tgttcattga
     4441 ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct aatacttcct
     4501 ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca tccattgctt
     4561 cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca ctctctacca
     4621 attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga attactgtgt
     4681 cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca aagattttat
     4741 gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg ataaaatcgg
     4801 ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc tgctgctcaa
     4861 gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg taaaaataat
     4921 gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt tcaatcatat
     4981 tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca ccgcctgctg
     5041 gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta ttattgaaca
     5101 tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca ctcattgtcg
     5161 gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc atagctagcg
     5221 aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct agaatcaagt
     5281 cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat acttgtgctt
     5341 tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt gctttggctt
     5401 gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg taaggcacag
     5461 ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata atgattcgat
     5521 aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca atcgcccaaa
     5581 tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga tctgttagtt
     5641 taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca gttgcagcca
     5701 aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg acggcagctg
     5761 ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg attcccgcat
     5821 ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga agaaattcag
     5881 aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat gcaaattcca
     5941 agccaatata tccagccccg atgatcacta atttttccgg taattgttcc tgatccatcg
     6001 cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt aaaatgattg
     6061 gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt tcaccattat
     6121 tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc actgtcccag
     6181 ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc aatttttctt
     6241 tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc aatgatttag
     6301 aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac ttttcaacga
     6361 ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg ccctttccaa
     6421 aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt ttttacctac
     6481 cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat ctgctttatt
     6541 tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt tcgatttcct
     6601 ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc tttggagtaa
     6661 gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct gatgccatcg
     6721 atatgcactt ctaatactac accgatctga tagccgccat catctaaatt ataaagagag
     6781 acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat atgttgtacc
     6841 gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt tgttccttct
     6901 gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc ctcgacttct
     6961 ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat tgtactgctc
     7021 ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt aaggcgcttt
     7081 ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt ttccaaaaaa
     7141 caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa tagcaatctt
     7201 ttctacataa tcttttatat gattgatttt aagatacata tataccagaa agttgacccc
     7261 ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct ccttttgctg
     7321 gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa gcaactagcc
     7381 tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa tgtccgaata
     7441 attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt tctatctgta
     7501 atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt ccgtatggct
     7561 cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc cctttttctt
     7621 cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt ggttcatcgc
     7681 tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct ttgatttgtt
     7741 catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc tttgcgatga
     7801 catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg cttgtttcga
     7861 tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg attacatttc
     7921 gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat ctttgtagat
     7981 gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt aatacgattg
     8041 tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca ctgacaactg
     8101 cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt gctaaaacag
     8161 cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga ttaggaaatc
     8221 ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta ttcaatactt
     8281 tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc caaccattta
     8341 gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa tcttgcgcat
     8401 aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt attttttgct
     8461 gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag gtaaagaatt
     8521 ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca gcatcgcacg
     8581 agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat tcgaaatcga
     8641 tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt gctggtccgc
     8701 ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg gttgatttct
     8761 tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg tctgtcgtca
     8821 agtttacttc atatccttca actggtaatg cgattcctcg aatatccgat ccattttgca
     8881 gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt tttccagctt
     8941 ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg tatctacacc
     9001 ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg ttaccacatc
     9061 ggcaccattt cgttcagtca atacgataac catgaactga acagactgga tacgcaaaac
     9121 ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg gctggttaaa
     9181 taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca tttctcgaat
     9241 gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc gcgcaagcag
     9301 ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg cttgtcgctg
     9361 tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa gtaatatttc
     9421 ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc taaccttttt
     9481 ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt ggatcgctga
     9541 tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg atgaatgctt
     9601 ctaatttctc aaacgttgct ggttcttcgg catagggccc gacgtgtaag atttgggcac
     9661 aaagaccttc ttccatcttt ttgaatacta cttgttcttt caagtcttcc ggaagtttgc
     9721 tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc tgtcggatca
     9781 tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac ttttcttgca
     9841 gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga atcagccaat
     9901 tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct actcttcgtt
     9961 taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatgat tttttaggaa
    10021 cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc cattcaattt
    10081 tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata cctctttttg
    10141 agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata agtgaagtaa
    10201 ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc tatttttata
    10261 atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg acaaaacaaa
    10321 aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt gcgggcgtat
    10381 tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt gatttaacga
    10441 ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc attgttccgc
    10501 tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct tcaaatctgc
    10561 ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc ctattgtttg
    10621 gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc catattatct
    10681 tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact ttgacaacgg
    10741 ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat tttacatgga
    10801 atcatatctt cctgtttctt gtgcccgttt tgtttctttc tttgattatc ggctggtttg
    10861 ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc gttttaggaa
    10921 tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca gtttccagct
    10981 ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga agtaaaaaag
    11041 tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc ttgtttttga
    11101 ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg ccgaatttcc
    11161 ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt cccggcgcag
    11221 cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat ggtccgaaga
    11281 agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta ttcctactca
    11341 taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg atcggtatcg
    11401 gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa gaactacaag
    11461 gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg gctacttcat
    11521 tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat gaagtgtcta
    11581 ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc gtaagctttg
    11641 ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat acggcaggtg
    11701 ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct tttttatgga
    11761 aaaaattgcc attatcggtg gcgggatcat tggtatgacc ctagccagtt acttagatac
    11821 atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta aagctagcgc
    11881 tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc gtctggctaa
    11941 agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc aaacgattta
    12001 cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat tggctgttct
    12061 tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc tttcacctga
    12121 acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta tcagtggtgg
    12181 tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag cacaaaaaca
    12241 aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt ggctcatcaa
    12301 gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc cggcacttaa
    12361 gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag ggcagctctt
    12421 agcatttcag acatcttata agatgagtca agactggcct gttgctatgc ttgacggaga
    12481 agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc atgaaaacga
    12541 tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta agagtacaga
    12601 acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc gtgtaggtac
    12661 ccgtgcttat acgtcagatt tttccccatt tttcggcgta ctgccagatg atccaagctt
    12721 actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata tcggctatct
    12781 attagccaac ttcttaaata ctggtcattg gcctacggaa gattatcaaa aggacatctc
    12841 cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag gatcccgagc
    12901 gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt tctgaatcaa
    12961 attctccaaa ctttcttttg cttcatctgg caagagatct tttcctatct gagcttcaat
    13021 gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg tatcctcaaa
    13081 agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac caaacgactt
    13141 gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt caggtttgat
    13201 ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct gtttcaaaaa
    13261 caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag gattagcaaa
    13321 tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg cgtctgatcg
    13381 ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga tcttgataat
    13441 tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt cgctaagatg
    13501 atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc cgtttcgtat
    13561 atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca cattctgaca
    13621 agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt tatcaaagta
    13681 agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag catcgtcatg
    13741 caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg attttccaga
    13801 accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat attgtttcat
    13861 cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa gcgtcaaagt
    13921 cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat tatggagagc
    13981 atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat cacaaaagat
    14041 ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga tcttcgaatt
    14101 cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt ctggaatcag
    14161 caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg tatggtaaaa
    14221 ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt tgctttgttc
    14281 cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa tgcttccatc
    14341 attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat gtgagattgt
    14401 taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg tcttcctatc
    14461 aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc gaatactgga
    14521 taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg tactcatggc
    14581 tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc gagtttttat
    14641 ttgtactgct gcgctgtggg aagcaaacga gttatttttc acacaaaaaa accgagaact
    14701 tgaataaaca agtttctcgg tttcatcatt ttccttttat cggcttagta ccagccgttt
    14761 gctaaccaga atgttttagc agcatcccat gaaccataac gagatgttac atattgttct
    14821 gctacacgtt cttgattggc agctgaatag tcgccgttta agtatgaagc atctaattga
    14881 taacggccga tgtaacgtcc gttagtagct gtgtatgaac cgcctgattc tttttgtgcg
    14941 atccattctt ttgcagagct tgaagttggc gctgttgcag cagctggggc agcaggagct
    15001 acttcaactg tttcaacagg tgcaacgtat gtatgttctt gcgttgctgt ttcagttgta
    15061 gatgctgtat tttctgttgt tttgttttct gttgttgctg aagaatcatt tgaagttggg
    15121 attgttaatt gttcaccaac ataaatgcgg ttgatgtttg cgatcgaatt tttttcagca
    15181 attgcatcga tcattgagtt gtctcctgca aatttttgtg agatcttaga taatgaatca
    15241 cctgatttta ctgtataaac ttcatcagcg tgagcatttg tagttcctac aaacattgca
    15301 gtagcaccag cagcaaaagt tgtacctaaa acaattttct taaatgaatt catattaaac
    15361 tccttttttt ctccgttttt tattctttcg acgctattaa ctttatcatt tagaaatatt
    15421 tca
//
LOCUS       DAZKOW010000066        15255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000066 DAZKOW010000000
VERSION     DAZKOW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.066"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1021
                     /locus_tag="ACG912_001967"
     CDS             2..1021
                     /locus_tag="ACG912_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0513277.1"
                     /translation="MSIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDHQLIT
                     GKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGWLFFF
                     TSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVVIGMI
                     ISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSV
                     FLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSMTNIL
                     LFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTV
                     RRLKR"
     gene            1018..1785
                     /locus_tag="ACG912_001968"
     CDS             1018..1785
                     /locus_tag="ACG912_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFQ0513278.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="ACG912_001969"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="ACG912_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFQ0513279.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="ACG912_001970"
     CDS             3750..4070
                     /locus_tag="ACG912_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFQ0513280.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="ACG912_001971"
     CDS             4070..4420
                     /locus_tag="ACG912_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFQ0513281.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /gene="hprK"
                     /locus_tag="ACG912_001972"
     CDS             4587..5525
                     /gene="hprK"
                     /locus_tag="ACG912_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFQ0513282.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /gene="lgt"
                     /locus_tag="ACG912_001973"
     CDS             5540..6370
                     /gene="lgt"
                     /locus_tag="ACG912_001973"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFQ0513283.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="ACG912_001974"
     CDS             6414..7439
                     /locus_tag="ACG912_001974"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0513284.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="ACG912_001975"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="ACG912_001975"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0513285.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="ACG912_001976"
     CDS             8517..8957
                     /locus_tag="ACG912_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFQ0513286.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="ACG912_001977"
     CDS             8960..9562
                     /locus_tag="ACG912_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFQ0513287.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9625..10728)
                     /locus_tag="ACG912_001978"
     CDS             complement(9625..10728)
                     /locus_tag="ACG912_001978"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFQ0513288.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG912_001979"
     CDS             10938..11939
                     /gene="ccpA"
                     /locus_tag="ACG912_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFQ0513289.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12294..12689
                     /locus_tag="ACG912_001980"
     CDS             12294..12689
                     /locus_tag="ACG912_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513290.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            12689..12940
                     /locus_tag="ACG912_001981"
     CDS             12689..12940
                     /locus_tag="ACG912_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513291.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            12983..13378
                     /locus_tag="ACG912_001982"
     CDS             12983..13378
                     /locus_tag="ACG912_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513292.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            13375..14631
                     /locus_tag="ACG912_001983"
     CDS             13375..14631
                     /locus_tag="ACG912_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFQ0513293.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            14643..14966
                     /locus_tag="ACG912_001984"
     CDS             14643..14966
                     /locus_tag="ACG912_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513294.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14959..15225
                     /locus_tag="ACG912_001985"
     CDS             14959..15225
                     /locus_tag="ACG912_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513295.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT         5242 a         2580 c         3225 g         4208 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaactc ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggcggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaataa atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt ctttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccccatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagcg
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta attgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 aaggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcagg cgttagaggc agaaatcaaa
     2341 gagttagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaatctttct gtgtctttag taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aatagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatgatgag atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg tgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat tgatcgatga ctatgagcgt acacacgctt ctacgattgc
     7921 cgttatgcaa gttcctcacg atgaaacttc aaaatacgga atcatcaacc caggggaagt
     7981 actggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttgggcgtta cttattgact ccagaaatct ttgatgtatt
     8101 atcagaacaa caaccaggtg ctggaaatga gatccaacta actgacgcaa tcgatacgtt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttatg acgttggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtctg acacatccag aagtaggggc
     8281 acctttgcgt gaatacatca tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 caatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aatctttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttca
     8581 tcgttcgcgt attgttgcaa gtttccaaaa ctttagaaaa agtagaccag actgtcgcag
     8641 aagcaaatac gacaattgag atcgtcacac aagacgtaga tcttttatct agacaagtag
     8701 aaggcttgct agtcaaaagt aatgagctgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcggaa cttaatcatt
     8821 ctagtaaaaa catcgtgacg aaagtcggcg gaatcggaaa aacgacagct aaagcgactg
     8881 ttgccggaaa agtcggtgga acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataagaa tggctaaaaa aggtggattt ttattaggtg cagtagttgg
     9001 aggtacagcc gcagcaatcg ccgcattatt gttagcaccc aaatctggta aagaacttcg
     9061 ggatgaattg gctgctcaag cagatgattt gatggacatg gcttcagatt acacagatat
     9121 ggcaaaagaa aaatcatctg agttaacagg tattgcaaaa gaaaaagcgc aaggtctttc
     9181 tcaacaagca ggggatctag caggcaatgt taagcagaag gtaagtgaat cagcagagga
     9241 agcaggaagt gaatcagatg atatcttgtt aaacctcaaa gaacaatcag ctgatttatc
     9301 taatcgtttc aaaaaaacag cagaagatct gaaaaagcca gcagacgaat taggtagtat
     9361 tgctaaagat acttcagaag atatcatgat cgatgtaaaa gatgctgcag ataaagcagg
     9421 agagacggtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgaca caaaagaaat
     9481 gttgaaaatg gcaaaagaag atgcaaaaga agctaaaaaa gaaatgaaag atacaatgac
     9541 agattcgact accaatcact aagattactc atttctagaa taaaaaaggt gagagcggac
     9601 cagctctcac cttttttatt ttcattactc taaaacaagt aattcttttg gtgtagtcgt
     9661 gaatggcaca caaccatcac ttgtcacata cacacaatct tcgatacgta cccctacttt
     9721 ttcaggaata tagattcctg gttcaagaga gaagcacatc ccttcttcaa tcactaagtc
     9781 gtttccttct acaagagaag ggaattcgtg gacggttgtt ccgattccgt gacctaaacg
     9841 gtgattgaaa tattcgccgt agccatagct gttgatcacg tttcgtgcga tttgatcaag
     9901 ttcacctgct gtcacacctg ggcgaactgc ctcttgtgcg gcaagctgtg cttcaagagt
     9961 gatgttatag attttttctt ggaactcagt tggtttttga taagcaactg tacgagtagc
    10021 atcactgcaa tagccattcc aaataacgcc aagatcgaat aacactagtt catttggtga
    10081 aaccttcgtg cttcctggtg taccatgagg gcttgcagca tttgctcctg caaggactaa
    10141 tgtgtcgaaa gacatttgtg aaacaccttg tttttttaat tggtattcga tttctgcgat
    10201 aattgcttgt tcagttacgc cttcttttac tgttttgaag ccaatttcaa aggcaacatc
    10261 ggcccattta ccagcttcga tcagtttgtc tttttcttct accgttttga tcagttgcag
    10321 acgctggata atagaagtta gatcggtgta agaatctgta cctgtcaaga tatcagagat
    10381 agcatcgaag cgatcgacag tcagagcatt cttttcaata cctacattac caggttgccc
    10441 atagcgtgcg cgaagctctt ttccgatgat ctcccaagga ttttcgctgt ctaagtagcc
    10501 acgtacatcg tacgcccagc tgctgttttg agcatcttca acttccagag caggagtgaa
    10561 aaggaaagga tcttgttcca gagggataaa aagagctagt acacgctcgt gaggatcact
    10621 ttcatatccg gaaaagtaag cgatgtggcc agggtcgctg atataagcca attctacttg
    10681 gtgttgtgcc atccattgac gtagttcatt gatttttacg ttattcattt ggcaacctac
    10741 tttctaaatt aattttgtat tcaataattg taccactgat tgattaaaag accaacgttc
    10801 ttaaaactga aaaaagtaaa aattttcatg aaaacagaga taaaacggtt gcaattgttg
    10861 agattatttg atatcctatt ttagaaatgt aagacgtatt ttcagaaaac atataaaata
    10921 tgaataggag aacacaaatg gaaaagcaaa caatcactat ctatgatgta gcgagagagg
    10981 caaatgtttc gatggctact gtttcacgtg tcgtaaatgg caatccaaac gtcaaaccag
    11041 caacaagaaa aaaagtcttg gaagttatcg atcgcttgga ttatcgtcca aatgcagtcg
    11101 cacgtggctt ggcaagtaag aaaacaacaa cagttggggt aatcattcct gatgtcagca
    11161 atatgttctt tgcctcttta gcacgtggta tcgacgatgt ggcaacaatg tacaaataca
    11221 atatcatctt agcaaactca gatggcaacg atcaaaaaga agtcaatgta ttgaataatt
    11281 tattggctaa gcaagtggac ggcgtgatct ttatgggtca ccatatcaca gacgaaatcc
    11341 gtggagaatt ttcacgttca aaaacgcctg ttgtactagc tggttccatt gatccagatg
    11401 aacaagtagg tagcgtaaac attgattatg tgtcagcaac aaaagatgca gtaaataaat
    11461 tagcaaaaag cggcaataaa aaaatcgctt ttgtcagcgg tgcgctgatt gatcctatca
    11521 atggtcaaaa ccgtctaaaa ggctataaag aagcattaag cgaaaacaat ctaccatata
    11581 gcgaaggatt gatttttgag gcacaataca actttaaaga tggtctgtct ctagttgacc
    11641 ggatccataa tagcggtgca acagctgtct atgtttcaga cgatgaatta gccattggta
    11701 tcttggatgg tttattagac cgcggcgtaa aagtaccaga agaattcgaa atcatcacaa
    11761 gcaacaattc tttactgaca gaagtagctc gtcctcgttt gacaagtatc acacaaccat
    11821 tatacgattt aggagcggta tccatgcgcc tattgacgaa aatgatgaat aaagaagaag
    11881 tcgaagaaaa aacgattatc ttgccttatg gaatcgaaga aaaaggatca actaaataat
    11941 aaaagatcaa tcatttaaag gttgttccaa aagtggtcaa cttctggaac aacgtttata
    12001 cgagaaaggg ggttatgaca aaattttgtc ataacccctt tttttttgtg actttaaaaa
    12061 aagagaaaat ggcggaaaca aaccttacgt ccatcgtata cgacacaatc tgtgcgtaga
    12121 tgctacattt tgttcactac tatcaaaatt actttaaaat gaaaacgtga taaaaaagaa
    12181 agtagaaaag tagataagta actctaaaaa atccaaaaaa atccaggctc gattgcgtac
    12241 ttatcttaaa ggagaaacaa tccatcatat ataacccgaa aggagacttc tatttgccta
    12301 agaaaagacg aaagaccata gtcatcattt tgaccattct tactatagga atcggaggga
    12361 aattttacat ggacaaaaga gaaatgcaaa gacagaaaga tttactcgca gtagaaaaac
    12421 agtccgtcaa agttctgaaa aatacctttg cggatatcaa ggaagtgaag attgaaaaat
    12481 ctgctcgaaa tgaaatgacg ggttcatacc ggatagttat tctaatgact aataaacaag
    12541 atcaatcgat ttatttttct tatagttttt ggaaagaaag aaatgagata ggaagttacg
    12601 gaatagttga tgaaaaaaag caaaaagaag gaaatacgct caataaagtt aaagtaactt
    12661 actctaatgg aaatgaggaa agtatttaat gattactgac aaacaatata gtgaattaag
    12721 tgatgctgtt tactggctag atcctaaaca tagagactat gtacctgaaa tgcaagaaaa
    12781 tttaagtttt aaaattaacg gcactatgta taaaatccta aaaatcaaaa actccttcga
    12841 cggcatgcag gcgatggctg tagcgccaat cgtccatagc aaattggaga aaaatttcaa
    12901 aaataaaaaa attcctgcaa actttcgcgt acttaaataa tgaaggtgtc aatacttaca
    12961 taaaaagaaa ggaggcacgt tcttgtcaag gaaaataaga aaactcagct tagccatttt
    13021 attaatcgtc atattaggga tcggagggaa agtctatatg gacaaaagag aagcacaaag
    13081 gcagaaagat ttactcgcag tagaaaaaca gtcagtcaaa gttctgaaaa atacctttgc
    13141 ggatatcagg gaagtgaagg tagaagaatt taaaaaaaac cctgttaccg gaagttatgg
    13201 tgcacttgtg acaatgacaa ataatgaagg gaaatctgtt tattttgatt atagtttttg
    13261 gaaggagaga aatgaacttg gtggttttgg cattgagaac agagcggtac aaaagacagg
    13321 agtcactata aaaagagtaa aagtaatttt ttctaacgga caggaggagt tagtatgaca
    13381 ataaacgata agcattacaa tgatattagt gagaaggttt attggctaga tcccaaatat
    13441 ccccgctaca atgagggata caaaaagaac tctgttaaag aatttgctgg tatggaattt
    13501 caaatcctac aaatcaaaga ctcattggac ggcatgcagg cgatgtctgt agcgccaatc
    13561 aagaacggta aagtggatct ttctcaagta gtgattgctt ttgcaggaac gaacttaagt
    13621 gattggaaag atttggaaac ggatgcaaga agtatcatga atctctacgg gaatagacca
    13681 ggagcaagtt tttctgaaaa agcaacaaca aacaagattt tatccgttag tggattgaaa
    13741 acagcggggc aaatcaattc agcgattgca tttgctaatg aagtaaaaaa ggaatatgga
    13801 gaagctgcta tttctactac cggccattca ttaggtggat tcttagcatt atatgtagca
    13861 gcagagaaac aatggaaaaa tgtcggcttc aacgggccgg acccgtatga tctcttatca
    13921 ccagaagcaa aaaaatgggt ggaagaaaat cccggtatgc tcaccaatta ccgaaatcac
    13981 gccgatttta tcggtaattt tggtggtaat ggcacaggtg ctgaagttcg gatcagtatg
    14041 gaaacagcat tcttgaatct gcttgcttcc cacagtttaa cctcttggaa gtttgatgaa
    14101 aatggaaagc tgattattcc tgaaaatgaa aacaatgtta aagctcgaaa acaacagaaa
    14161 gaaaaccaag cgacccagtt gttttcctat cagttgcaag aactgaacaa catacgtaaa
    14221 aaactacgat taagtggcgg ccggctgaca gttaatgaac aaatttactt agatgacaca
    14281 caagctaggt tagtagtgga aaagttatcg gcaaaaatga aaagcgctat ggaagaagtt
    14341 atccgcatca atcaaagagg aatttttgag ctagagaaaa aatggcaaga aggcttgcgg
    14401 caagcacgtg ttgtagcccc ttcgttgact gaacaggaaa tcatagaagc attagctagt
    14461 gtgggcgcca caaaagaaaa cttagtcgat tctgcgaaag aagtctatct gctacgaatc
    14521 aataaggcaa gacgtatggg ggaggccttt gatacattgg cgaaggagat ccaagacaaa
    14581 atcaatgaaa tcgtggcacg agatcgagaa ttagcacagc aattcaatta aaggagaagt
    14641 agatgaaact agaagaattg gaaagagatt atcgaagtga acgaagacaa ttagaagctc
    14701 aagaagatga attgatccac tttaagaatc aaggattacg attgatggag caagaggagc
    14761 agcgactgca tcatgtatta aagaacttag ctttagataa tgagcctctg tttcaagcaa
    14821 gacgaagtta ttcgaattat caggagcatt atcaacaagc cctacaagta gaacgacgaa
    14881 aactctatca gcagcaagat gaactagaac gttcctatcg tcaaaaaatc agacaaataa
    14941 ctatggagaa atcatcttat gaatgaattt ttaatgcaat tactaaaaga attagagaaa
    15001 aatctacatc gatcaaatgt acgttcagtt ggtatgggga tatctcagca aatttgggca
    15061 gaaaaacgat tgacagatca aaactaccaa atgactgcag aagaagctgc tcaagaattt
    15121 tctaaccgac atgtacactt gaaaggatca gtaaaagggc aatttgcaga aaaactaact
    15181 gaattattcc aaagacaaga aaagaaatta aaacagtttc gttagtaaag ttatatagaa
    15241 aaattttgtg aaaat
//
LOCUS       DAZKOW010000067        15231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000067 DAZKOW010000000
VERSION     DAZKOW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.067"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            278..787
                     /locus_tag="ACG912_001986"
     CDS             278..787
                     /locus_tag="ACG912_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0513296.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="ACG912_001987"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="ACG912_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0513297.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="ACG912_001988"
     CDS             2322..3302
                     /locus_tag="ACG912_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0513298.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="ACG912_001989"
     CDS             3424..4842
                     /locus_tag="ACG912_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFQ0513299.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="ACG912_001990"
     CDS             4913..6382
                     /locus_tag="ACG912_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0513300.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="ACG912_001991"
     CDS             6392..6859
                     /locus_tag="ACG912_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0513301.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /gene="scrK"
                     /locus_tag="ACG912_001992"
     CDS             6895..7767
                     /gene="scrK"
                     /locus_tag="ACG912_001992"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFQ0513302.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7849..10383
                     /locus_tag="ACG912_001993"
     CDS             7849..10383
                     /locus_tag="ACG912_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0513303.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10539..11312
                     /locus_tag="ACG912_001994"
     CDS             10539..11312
                     /locus_tag="ACG912_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0513304.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11305..12096
                     /locus_tag="ACG912_001995"
     CDS             11305..12096
                     /locus_tag="ACG912_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0513305.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12106..12597
                     /locus_tag="ACG912_001996"
     CDS             12106..12597
                     /locus_tag="ACG912_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFQ0513306.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12615..13025
                     /locus_tag="ACG912_001997"
     CDS             12615..13025
                     /locus_tag="ACG912_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0513307.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13029..14429
                     /locus_tag="ACG912_001998"
     CDS             13029..14429
                     /locus_tag="ACG912_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HFQ0513308.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14452..14826
                     /locus_tag="ACG912_001999"
     CDS             14452..14826
                     /locus_tag="ACG912_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0513309.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2137 c         3045 g         4762 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga cccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttt caattagtaa ttgaagggac gcctaaaagt gaaaaaaaaa
     7861 atgaaagatg aagtattaga gtatatcaaa aataatttaa agtattacga tttttctgct
     7921 caagatattg caatgaaatt ttgtattaaa agaaatgtcg cttcccacta tttgaatcag
     7981 ttattttcag atggaaaatt actaaagaat gattctgtta gaccagtaat gttcaagtat
     8041 aatcaacaaa agccaaaaga ctgcttttct aagtttattg gagcagacat atctttgaaa
     8101 tccactatag ataaatgcaa agctactgta atgtatcccc ctaatggcct cccattaata
     8161 ataaaaggaa acagtggcgt tggaaaaagt tttttagcaa gtttgatcta tcagtatgct
     8221 cttgatagaa aagtaattca taatgacgca aaatttgttg ttgtgaattg tgcagattat
     8281 gcaaataatc cagagctatt gtcagcagtt ttatttggat ataaaaaagg agcctttact
     8341 ggggctgaaa aagatactgc agggatgcta tcttctgcta atggaggata ccttttttta
     8401 gatgaggttc acaatctaag tgcagaaaac caagagaaat tgtttcttct tatggattct
     8461 caaaagtatc gtaaacttgg tgaaagtgtg caatgggagt atgcaaatgt tcgccttatc
     8521 ttagccacaa cagaagataa aaatacctct ttgttagcaa cttttagaag acgcatccca
     8581 gctgaaatca ctctgccaga ttacaataat agaagtacat ctgaaaaaat tcaattatta
     8641 tttgaatttc ttaaagatga agcaattaca attgattcaa aaatattttg ttcgattgaa
     8701 ctttttactg atcttttatc gaaaacattt gaaggaaatg ttggagaatt gaggaatgaa
     8761 atcaagcttc tttgtgcaga aggttattta ggaaataaga gaaagtcttc aatttattta
     8821 ggggacaaac tagacagcgg tttttggata gatccagaaa ttagtataga tttaaaaaaa
     8881 atgtttctta aatcactttc tgaaattgat ttgattgaac tttttcaaaa tcacatttca
     8941 attgaagaaa gcatgagtca attaagagat agaatactta aagagtgtgc aggttcttct
     9001 gagtataatt atttagaaag tgacgaattt atagcattaa gaaactatgt tgttaataaa
     9061 atatcaccaa ttattgattc gacaggtcta tgtttattag atgaattctt ggcggatatc
     9121 agtctattta taatgtttat tgatgtaatt gaagaaacat caagaaattt tttgctgtct
     9181 aagttcagaa aatttcgtat gaaaaatgat aaacaaaaat tattggcaaa tgagatttgg
     9241 agtagtttag aagtcgaaga aagtaaacaa gaacttttgt tgaaatggat actttttctt
     9301 gtaaagaagt tctatgtgaa aataccagaa acccattgtc tgatagttat gcacggtaaa
     9361 ataacagctt ctgcgatcgc aagggaaaca aataagttgc tgaatacata tgtttatgag
     9421 gctttcgata tgccaattga aggtgagact agtgacttaa ttaacctaat caacaatttt
     9481 tgtaaaagta tagatacgac gaatggactc attctgctag ttgatatggg atcattggaa
     9541 caaatgtatg aaaaaattga atccaatgta ataggtgatt tggtaatttt aaacaatgtt
     9601 tctacagctt tagcgattga atgtgggatt caagtttgcc aaaaaaagcc aattagtcat
     9661 ttttaccaga tggattttga ttcgtttcaa gttaaagttc agtattataa aggattatca
     9721 caaaagaaaa atgtaatagt atcttgtctc tctggtgagg ggatttctga aaaagttaag
     9781 gatattttaa aacggtatct cgataatcaa gtggagattt tgatttttga ttttaacgca
     9841 ttaaagaaaa tagcaaagga aaaagatact atgattttta agaatacaat ttgtgttctt
     9901 tcaaccactg aattttacat tcaaggaatc gattgtttaa atttagaaaa tctaattaat
     9961 gggaatcaaa ccttagaaaa attaaataag tatatggact cggatagctg tgagttctgc
    10021 ttaaatgaat tagttaaact ctttactatt gaaggcgcta gtactaagct tcgcttttta
    10081 gattccaaaa aagtattcaa tgagatagaa aaagtactat atttgtatga aaaatattac
    10141 gatgtcaaaa tcccaagttt tctgagaatt aatctatttc tacatctttc tggaatgatt
    10201 gaacgaatta tgattggtga tggaataaca aatgaacatt tgaaagaggg agcgcaacta
    10261 tttgatactt ttttaaatgt atcaaaagat tttttttcag aaatcaaaga tatttactgt
    10321 attgaaatac ccaaaggaga gtatgagtta atctatacaa tttttgagca gactattttc
    10381 taaatagcat actagcgtct aaaaaaatga aagggtgtcc aaagtatatt tagacgctca
    10441 tttaattaat atttactgta ttttcccgct ttattggata atgttagttg gcacagtaat
    10501 tgcttatata aaagtataag aagaaaggag agaacaaaat ggcgttttgg caaacatttt
    10561 tagttgtgtt gtatggattt ttcattaact gggataagga agggcccaaa atcggaacct
    10621 cccaacctgt cgttgcaggt ttcttgaccg gacttattat gggagatatt caaactggac
    10681 tttatatcgg tgggactttg caattaatga cactaggtat tactagtttt ggtggtgcat
    10741 cagtaccgga ttatcaaaca gcagcattga tcggtactta tttagcaatc tcaactaacc
    10801 aggatgcttc tataggaata actttagcaa taccggtagc aatgttaatt gtacaactag
    10861 atgttctaaa gtggtcaaca aacatttttt ttcaacaaaa ggcagaaaaa ttcgcacaaa
    10921 aagaagatta tcaaaaatta gagtggatgc aatatttggc atcaattaat acaagctttg
    10981 tatctggaat tcctgttttg ttaactgtga tattaggccc aacgttagtg ggaagtgcaa
    11041 tcaattatat tcctgaatgg gttaccggtg gtttaaaggt agcaggggga ctattacctg
    11101 ctgttgggat aggaatgcta cttaaatatt tacctacaaa agattacttt gcctatttgt
    11161 tagttggttt cgttttggca gtttatttcc aaataccaat tttagggatt tcgttacttg
    11221 gcgctgcaat cgcactaatc gtatataaaa atagatcaaa taatactcaa gtagcaccag
    11281 ctgcaacagg aggttttgac gaagatgaat aagaaactga caaaaatcga caaacaagta
    11341 attttccggt ggattctcaa tggtgcaact tcattaaatt atgaaaaaat ggaagggttg
    11401 ggttatgcgt attctatgct tccttttatc aaggacacat ataaagatga tcctgaagga
    11461 atgaaacaat ccgttttagc tcacctacaa ttttttaaca ctacgccgta tacagcgcct
    11521 tacattattg gtatgaatgt tggaattgaa aaagaagaaa aaaataagtc tttggaagca
    11581 gtaagtgctc tgaaaacagg tctaatgggt cctttagcgg gaattggaga tagtgtattt
    11641 gtagttattc catggactat ttttggagca attgcagcta atatggcatt ggaaggtagt
    11701 tttgttggaa ttattttgtg gatactggca tcaattttat taaggattac tgcttatcct
    11761 ttataccttg ctgggaaaga atcgggtgcg agcctactaa ctcaaattga aacaaaatta
    11821 aagtctatta ctgatgcagt atcaatttta gggttaatgg tggtcggtgc actcattgcc
    11881 acagttgtaa aagtgaatgt tggctttgaa tttaaacaag gcgacttaac aatgaccgga
    11941 aatgaaatct ttgatcaagt tatgcctggt ttattacctg catgtgtagt agcaattgtg
    12001 tattatttgt taggacgaaa ggttaaacca gtttatgtaa ttctattaat cttagtagtt
    12061 tctattgcgt taagagcgtt aaatattttg aagtaggtgt tgaatatgga tgaaaaaatg
    12121 aaaggtatta tacatgttcg tattgatgat cgaatgattc atggacaagt agcaacacaa
    12181 tggacaaatc aattgagtgc taacagaatt atggttatta ataatgaagt agccaatgat
    12241 gacgtaaaaa aagctgtagt tcgcctcgct gcaccaccta atgttagaac ttcaataatt
    12301 acaagggaag tagcagttaa aaatattctt tctggtaaat atgaaggaca aaaagttctt
    12361 attgttgcag tttctccaat cgatgttaaa tttttgattg ataacggttt accaataaca
    12421 agtgtaaacg taggaaattt atcaagaagg aatggaacag aacgcatccg tccgacgata
    12481 aatattactg aagaagagag agaggctttt aaagaactaa ttagtgatgg agttgaagtg
    12541 acagtgatac aaactcctag agattcaaaa gaatttttaa aggattttat ttattaagaa
    12601 cagaggatga gtggatggag tttataatta catctcatgg aaatattgcc attggatttg
    12661 tagatacatt aaaaatgttt tttggcgata atttaaattg tcatgtggta acacttggag
    12721 atgcaggcat tgaagaattc cgtgaaaaca ttgaaaaaat agtttctcaa gtaaaaaatc
    12781 aagaagttat ggtatttgct gacttgattg gtggaacacc tttcaacgaa tttgctaaaa
    12841 gaagtgaagt atttcaaaat gggtttcaac ttcttggtgg catgaattta ggaattttga
    12901 ttgaggctgt caatttgaga ttacaaggaa aaaaaagcga tgatatcatc gaaaatctaa
    12961 gaaatatgaa tacaattgcg tgtcttgcag atatgcaaag acatactaac gaagaggatg
    13021 agtgataaat gcaaaaaatt ttttacaagc ctaaagattt ttggtttggg gattgcatgc
    13081 ctttttataa agatggaact ttttatctat ttcatcaacg agatacgaga aatcctaaac
    13141 ctttaactga tccttttggg tggtcactag ttacaaccac tgattttgtg aaatttgaag
    13201 aatacgggga agtaattcat cgtggtggca acgatgacat tgatcaatac atttacgctg
    13261 gaagtattgt ccaaggaatc aatgagtatt tagctttgta tactggacat aatcgcaaag
    13321 ctaagttgga tggaaaaact tcagaagtgt taatgattgc aagttctaaa gatttaattc
    13381 attggaaaaa ggatggcgaa gcagcaaaac ttgttcctca agaaggatat gataagaatg
    13441 attggcgcga tccttttgca atttttgatc aagatcatgg aaaatacgtt ttgatactag
    13501 gagctagaaa accagcaagt aagaaaaagc cgacggggag attggtctat tttgaatccg
    13561 aagatttaaa agattggaat ttcaaaggag actactgggc taataatgaa tttaacatga
    13621 ttgaaatgcc tgatatattt gaaattgatg gaacgtggta tttacttttt tcagagtatt
    13681 gtgaagataa aaagactaaa tatcgagtcg gagcaggatt attttcagat tggaaatcac
    13741 cagtagacga agctttcgac ggaaaagcct attatgcagc aagaacagtc ggaactaatg
    13801 aaactggccg ttttctaact ggctgggtgg caacaaaaga aggggagaac gatcaaaata
    13861 actgggattg gggcggtgca cttgttccac atcaaattat tcaaaagaca gacaaaactc
    13921 ttggtgttaa actgcctgaa aaaataattg agaattttaa agaaatcact ccattttcaa
    13981 gttttaagtt agagaaagaa tttggaaaaa aagaaagagt aattgttcct aaaatcgaag
    14041 attattttct atttgaaaca actttgacta ttacatcgtt aacaaaggag tttgctttaa
    14101 aattatttgt agatagtgat actcaagaag gatacgaatt tcgtatttca ttgattaata
    14161 atcagcttac tttcggaaaa actccgaact atccttggcc tcaaatgttt gataaggggc
    14221 tgcaacgacc actatttttg gaaataggca aagaatatga actaaagtta attgttgatg
    14281 atacgatagc tgttttatat attgataatg ttgctttaag tactagaatg tatgataaag
    14341 tcggaacagc attaagtatg actgttacgg agggagcaat caaggtagat aaactagact
    14401 attcaataaa ttatgaatgt ggattttaaa acaattgaag ggatgatgta agtgaaatta
    14461 aatggtttac aacatattgg aattccaaca aaagattttg gagcttcaaa aatcttttat
    14521 gaatccttag ggtttagtct gattaatgaa gaaatgaatg tgggaagtaa agtcggcttt
    14581 ttcgagttga atggaacgat aatagaaata tgggaagata aaactactga gtgtagagga
    14641 gccattgatc atctagcatt agatacggat gacatagatg aggtattcaa ttcaattcaa
    14701 gatttaggat atgaattatt agataaaaat attatggaat taccgttctg ggaaaatgga
    14761 atccgttact ttaatttcta cggaccaaat catgagatta tagagataag ccaaatgaag
    14821 aaataaatag ataacgaagt gctctaagtt ggtatgaacg aaaagtactt cattttttgg
    14881 tatcagcatt tcaatagttt aatgagaatt tactatttca aattaattca aagtgacagt
    14941 attttttact tatttggttt caaattttgt aatccttcct atttattgga atacactcca
    15001 cattttgtat acaagtcata tcatgtttta gcaaagctct taccaagcaa agttataatg
    15061 atgttttcca aagtttgatc tttcgatgga ggctaacaca tattttttgc aggacagcca
    15121 aaattagatg tatagaatag agtctgtgag gcgtatagac agtttattgt ctgtatgcct
    15181 ttttttattt tgaaaaattt ttagtatttt tcgaaaaatg ctcactccta g
//
LOCUS       DAZKOW010000068        15149 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000068 DAZKOW010000000
VERSION     DAZKOW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.068"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..712
                     /locus_tag="ACG912_002000"
     CDS             <1..712
                     /locus_tag="ACG912_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513310.1"
                     /translation="AFLITKNTILYNVAFILLNLLISVVFAIIMSELRNKRLVKVYQT
                     MSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGT
                     WKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVG
                     NIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVL
                     LMITNTIARRFDSDSALF"
     gene            724..1644
                     /locus_tag="ACG912_002001"
     CDS             724..1644
                     /locus_tag="ACG912_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0513311.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1685..3142
                     /locus_tag="ACG912_002002"
     CDS             1685..3142
                     /locus_tag="ACG912_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0513312.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3487..4101
                     /locus_tag="ACG912_002003"
     CDS             3487..4101
                     /locus_tag="ACG912_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFQ0513313.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            4094..5818
                     /locus_tag="ACG912_002004"
     CDS             4094..5818
                     /locus_tag="ACG912_002004"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFQ0513314.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5815..7266
                     /locus_tag="ACG912_002005"
     CDS             5815..7266
                     /locus_tag="ACG912_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFQ0513315.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7739..9892)
                     /locus_tag="ACG912_002006"
     CDS             complement(7739..9892)
                     /locus_tag="ACG912_002006"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFQ0513316.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9894..10940)
                     /locus_tag="ACG912_002007"
     CDS             complement(9894..10940)
                     /locus_tag="ACG912_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0513317.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            11090..12379
                     /locus_tag="ACG912_002008"
     CDS             11090..12379
                     /locus_tag="ACG912_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFQ0513318.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12392..15094
                     /locus_tag="ACG912_002009"
     CDS             12392..15094
                     /locus_tag="ACG912_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFQ0513319.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
BASE COUNT         5106 a         2645 c         3050 g         4348 t
ORIGIN      
        1 agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc ttttgaatct
       61 gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac gactcgttaa
      121 agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta tcagttactt
      181 tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca ctggacatgg
      241 aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct tggtgttcat
      301 tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg ttatgggaat
      361 cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc agcagatcaa
      421 acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt tagccgtagg
      481 aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt ccggtgcatt
      541 atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat ccacaggaga
      601 ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg tcttgttgat
      661 gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct aaggaggaaa
      721 aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa gacggctaat
      781 tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc atttatattt
      841 gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta cagtttttgg
      901 ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca ggataaaatt
      961 ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat caatagcacg
     1021 gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg tcgttttttt
     1081 accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa ttatcttgtt
     1141 atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc aatggctgtc
     1201 agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc tgattcgatt
     1261 attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca gatcgtttta
     1321 ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg ttactggaat
     1381 gactggttca atgctttact atacatccaa gatgataagc tagttccact acagtatctt
     1441 ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc aggaagccaa
     1501 ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat cgtagtcatc
     1561 tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt caaaggtcta
     1621 acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat taggaggaag
     1681 aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt tgttgggaac
     1741 attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca attcggacac
     1801 tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga tggaagtagc
     1861 aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca ttggctgggg
     1921 cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg atatcgcctt
     1981 tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga ctgaattagc
     2041 gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta aaggaaatct
     2101 ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc aacaagtact
     2161 tacttttaac aaagctttac tagataaata cgatctttca attgatggaa tccaatctta
     2221 cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca cggcagcttt
     2281 tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag ggaatacgtt
     2341 accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat acgataatga
     2401 aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac tcattccaag
     2461 tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc taatgcgtgg
     2521 agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag caaaacaaga
     2581 acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac aaatggcaaa
     2641 ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc tagggctgct
     2701 taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag aagcatggga
     2761 aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc caaatacaca
     2821 tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt ctattacgga
     2881 tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac cgattttagg
     2941 attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta atgtcatgaa
     3001 tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat taccgaaatt
     3061 gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc aaaaacaata
     3121 tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac aaaagacgtg
     3181 tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt attaagctaa
     3241 atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact cgaaactaag
     3301 cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg gtattcgctc
     3361 acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa tcaggtcaag
     3421 gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat gtttggagga
     3481 gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt cattataaaa
     3541 ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg attaggtgcg
     3601 gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa agaaatcaca
     3661 ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct gctatttagt
     3721 ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt tcaaatcaaa
     3781 gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg cctatttgct
     3841 acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc taatctgcta
     3901 aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat aattattggt
     3961 agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt tggtgtcatt
     4021 ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga attagataaa
     4081 cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct tactaaatcg
     4141 tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta ccgttatttt
     4201 tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa gtgatgccgt
     4261 tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca tcaaaaacca
     4321 gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa aacccataga
     4381 tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag tttctttccc
     4441 aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat atatcgtgtt
     4501 gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg aaataaaaaa
     4561 ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac aaggaggagc
     4621 tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga ttttagaaaa
     4681 tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa atcgactata
     4741 cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa tgatcatcac
     4801 agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa acaattttgt
     4861 tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata aaaaaagtgt
     4921 aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta ttttagacaa
     4981 tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag tatctatcgt
     5041 catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg acataacaaa
     5101 cacggaatat gaactgaagg aactttcaat cggtatcaac gaaatgttgg atagcatcaa
     5161 tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac atatgcgggc
     5221 gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata tccgaatgta
     5281 tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt ccgctttgct
     5341 tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg acttttgcga
     5401 aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc attttatgat
     5461 cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat tagttgaaaa
     5521 ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg tcaaagtctt
     5581 gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga tgactgaaaa
     5641 acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc acggttctat
     5701 cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt attatatgtc
     5761 gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg agggatgagc
     5821 gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt aaaaaagata
     5881 attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc caaagaagga
     5941 ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac aatgccagaa
     6001 ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt tgaatttatg
     6061 attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt aggcgccgtg
     6121 aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa gaaaatcaaa
     6181 acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca agaactgtta
     6241 ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat aataggacaa
     6301 ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca tgatactctt
     6361 atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa ttacggagac
     6421 cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg ttgttttgtt
     6481 caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac agattctttg
     6541 gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca tcaattttat
     6601 ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga tcaaaccatc
     6661 gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tagaagtcgc tcaaaaaatg
     6721 atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga tatacgatat
     6781 tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga agatgaggaa
     6841 tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact tcagcagcta
     6901 cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa tgtggaaaaa
     6961 gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga agtgtcagaa
     7021 aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac gaaaaaaagt
     7081 ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct tttgcacagt
     7141 aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac gtatttctct
     7201 aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa cagtggagaa
     7261 tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta gaaatctaat
     7321 tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc gataaacgga
     7381 gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg aaagccattt
     7441 tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc tccactaaac
     7501 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat gaggaactat
     7561 tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc tctcaataaa
     7621 cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa tatgaggatc
     7681 tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca acctcttgtt
     7741 aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta gggtgaggga
     7801 tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg gtaaaatata
     7861 atttcgtata ttctttgtcc atccgtttga ttttatggat aaattgttgc tgcggccgat
     7921 taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca gccttgtcaa
     7981 ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa aatcccagac
     8041 tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg taacccattg
     8101 gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc atttcaggtt
     8161 gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa atagctaatt
     8221 gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca tatcctcctg
     8281 ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat ctctcactgc
     8341 caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg ctttgccaag
     8401 cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct ctgaacttgc
     8461 catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt tgatagttca
     8521 atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg gcaacttggc
     8581 taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg tggtagtgat
     8641 taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa tttggatgtt
     8701 tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc aagtctcgtc
     8761 ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca ggcataagtc
     8821 cacgttcatc aggagacagc cattttggca aatagccact ttctctatag ctgttcaaaa
     8881 aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga ggataaaccg
     8941 ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt atagatcgac
     9001 tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat gtttgtggaa
     9061 ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga tcatggtgtg
     9121 tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca aggtaagtct
     9181 ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag ctgataaaag
     9241 atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg atcgagtcgc
     9301 tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat ctcaatataa
     9361 aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt ccttccactg
     9421 tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt aaaccgctgt
     9481 catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc aatgtagaac
     9541 agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata ctttcttctg
     9601 ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca ttgatcggcg
     9661 taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta agacggtagc
     9721 cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc gtagtttgcg
     9781 gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc ccattagaga
     9841 aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc attttatttc
     9901 tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga tggtcgatct
     9961 tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg ttcaaaatct
    10021 tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc atcaaatccg
    10081 ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt catggtctgg
    10141 atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt gatataagaa
    10201 agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt caaaccagct
    10261 ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg atgagtctgc
    10321 gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga ggcagcaagc
    10381 gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata gattttttta
    10441 tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc aagatgttgt
    10501 tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc ttttgctgtt
    10561 ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc ttgcatcaca
    10621 gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg aagaagaaac
    10681 agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc ggatttaacg
    10741 aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc agtcaatgca
    10801 cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc agttggtagt
    10861 tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt aaatattgtc
    10921 tggtacaatg gttgattcat ataaacacct cttaatgata tatcatattc aaataatacc
    10981 ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat aattggctgt
    11041 atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag tggcttataa
    11101 agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat gtggggatat
    11161 ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt tgacaacagt
    11221 caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag cgatgtggtt
    11281 aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag atgaggaatt
    11341 agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata tcgatccata
    11401 tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc atacaaaaat
    11461 gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc caatacagtt
    11521 ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat cttttattga
    11581 aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac actctcctta
    11641 tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg ggcgaggtgc
    11701 aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg atacttgccg
    11761 ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt atgtggaaga
    11821 aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga aattgaagaa
    11881 agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag ggcaaacgat
    11941 ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca gtaatgtgcc
    12001 caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag attatctgca
    12061 aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa agtacgcaaa
    12121 aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat tagctatgga
    12181 aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac ttgtagcaac
    12241 tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg aaaattatac
    12301 gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg attactttga
    12361 tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata tcatttctca
    12421 tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc gactgattga
    12481 attgatagac gatctactag agctatttga agttgatccg tccttcaaca gttttcattt
    12541 ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa aaagacaggc
    12601 aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata tcttgcaaga
    12661 tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca tggaagaaag
    12721 ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg gaaatatggg
    12781 acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg gccgtggtgt
    12841 aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt cccaatactc
    12901 agagatgtgg tggaaaggac ctgatcaaac agcaatcttt ggtctgctat ttgccaattg
    12961 gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct tttggaagca
    13021 aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga tgaatggtgt
    13081 tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa atgaactgtt
    13141 tccagattac gagtttattc attctaattt tactgactat ctggaagcag ttcaaaaaga
    13201 tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa cggatggctg
    13261 gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga ataccaaggt
    13321 ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg aagttagtgg
    13381 aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa atcatcctca
    13441 cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac ctcgttttga
    13501 aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc tgaccgcagc
    13561 aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa atactgcagg
    13621 aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa gattcgctga
    13681 aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta agtatcatgt
    13741 agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg tccaatttgg
    13801 ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga tcaaagtgac
    13861 tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg tagaagggga
    13921 agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca tcgaaaacgg
    13981 ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc gcaaaaacaa
    14041 acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa atgagtatat
    14101 ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa actctgaagt
    14161 aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg aaatggaaat
    14221 ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg aatttcgtta
    14281 tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa tcatgacaat
    14341 cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga tgaaagatca
    14401 tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag cagatagtat
    14461 ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc ccacgaatcc
    14521 gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa cagtcggaaa
    14581 ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga cgctacttcg
    14641 cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac agtgtctagg
    14701 tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat tctctactta
    14761 tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac atcatgaagg
    14821 aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg caattacagc
    14881 tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata tgtctagtca
    14941 tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga atctgcttga
    15001 agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc ggacaatcgg
    15061 attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa atttgtgtgt
    15121 gcggtttcag atggtggaga aattttaga
//
LOCUS       DAZKOW010000069        15072 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000069 DAZKOW010000000
VERSION     DAZKOW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.069"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            78..686
                     /locus_tag="ACG912_002010"
                     /pseudo
     CDS             78..686
                     /locus_tag="ACG912_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            706..1734
                     /locus_tag="ACG912_002011"
     CDS             706..1734
                     /locus_tag="ACG912_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0513320.1"
                     /translation="MNRIWNKSHRNNCSLLLILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILIRNSEKESSPNLKLKKTYADQSDGRNVIKANLQNPNAS
                     YMFGLTTSVEIYKKETKEPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKG
                     AWKEKRWNLHTDFEITDDAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFI
                     LKERRKKK"
     gene            1731..2489
                     /locus_tag="ACG912_002012"
     CDS             1731..2489
                     /locus_tag="ACG912_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0513321.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPNEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QNGTDSTGTATQVQNFVQVTDKSGIYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGTPSTISGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEGKA"
     gene            complement(2575..3315)
                     /locus_tag="ACG912_002013"
     CDS             complement(2575..3315)
                     /locus_tag="ACG912_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0513322.1"
                     /translation="MKKYFIIEDNQFHSEWIIKELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            3376..4629
                     /locus_tag="ACG912_002014"
     CDS             3376..4629
                     /locus_tag="ACG912_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0513323.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGFIALMVVTLL
                     FPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGLRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            4924..5178
                     /locus_tag="ACG912_002015"
                     /pseudo
     CDS             4924..5178
                     /locus_tag="ACG912_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            5300..5626
                     /locus_tag="ACG912_002016"
     CDS             5300..5626
                     /locus_tag="ACG912_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFQ0513324.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLQLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEF"
     gene            5694..6407
                     /locus_tag="ACG912_002017"
     CDS             5694..6407
                     /locus_tag="ACG912_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFQ0513325.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYLVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PIFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEEKEIVTLITCGDMDAVTRLVVQGELESVTP
                     IKDATDDMRSAFNLETKTF"
     gene            6717..7569
                     /gene="licT"
                     /locus_tag="ACG912_002018"
                     /pseudo
     CDS             6717..7569
                     /gene="licT"
                     /locus_tag="ACG912_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            7690..9555
                     /locus_tag="ACG912_002019"
     CDS             7690..9555
                     /locus_tag="ACG912_002019"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0513326.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            9569..11012
                     /locus_tag="ACG912_002020"
                     /pseudo
     CDS             9569..11012
                     /locus_tag="ACG912_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            11120..11821
                     /locus_tag="ACG912_002021"
     CDS             11120..11821
                     /locus_tag="ACG912_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0513327.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            11947..12459
                     /locus_tag="ACG912_002022"
                     /pseudo
     CDS             11947..12459
                     /locus_tag="ACG912_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(12857..13066)
                     /locus_tag="ACG912_002023"
     CDS             complement(12857..13066)
                     /locus_tag="ACG912_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFQ0513328.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            13307..13882
                     /locus_tag="ACG912_002024"
     CDS             13307..13882
                     /locus_tag="ACG912_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFQ0513329.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            13882..14322
                     /locus_tag="ACG912_002025"
     CDS             13882..14322
                     /locus_tag="ACG912_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFQ0513330.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            14338..15039
                     /locus_tag="ACG912_002026"
     CDS             14338..15039
                     /locus_tag="ACG912_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0513331.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         5083 a         2501 c         3179 g         4309 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttaccca gaaaatcaac tttttgcagg agatgtatta gaaattgtat
      121 taaacgacca agcaggagaa gctgctgcta agggtgtgca gaacaagcca agtaccaacg
      181 atgaaattgg gaatcacaat ccaagtgata cgccagttgt ttatcatgat gccccgcctt
      241 ttgcacctgc tacgaagatt gtagtagaag gtggtctctc atttcttgcc ccttccaaac
      301 tcgattgtgg tcagatcaaa gcaactgggt tgacgcaaga agcatttggc caaatggata
      361 tacaggaaaa attatggatt tatgatcagc gagaaacaaa aagtccatgg atcttaacga
      421 taaaattatc tcagcctttc acaaccgatg caggattttc tatgcaggat gtcctatatt
      481 tcaagaaagc tgaggagtat caattagtga atgaagaaga actggaaatt gtcagaggac
      541 aaaattcaac gaatgtgcaa acagattatt cagaatattt aaataatggt cgggcgttta
      601 aacttgtctt aaccaaagaa aatcagatag ctggtaatta ctcagcagag atgacatgga
      661 cattagcaga tgcgccgaca gaataaagat caaggagaat gattgatgaa tcgaatatgg
      721 aataaaagtc atcgaaacaa ctgctcttta cttctgatcc ttctccttgg attcatcctt
      781 tggcagacac ctgtccatgc ctccgaattc aattttgcgg tagattttga attaccggct
      841 aatcagattg ataagaacaa aacatatata aatgtaagga tggagcctgg gaaaacacag
      901 gaaatcccct acacggtctt taaccgaaca gataaatcag taacagttga gacattgatc
      961 cactccgcta cgacgaattc aaacggtgtg atcgaatatg gagaatcaaa aagtaaattt
     1021 gatgaaacat taagttatga tatgaaaaac ttagcaaaaa cagtgggaga aataactgtc
     1081 cctcctcaaa gtgcggtcac tgagacactt tcactgaaag ctccagaaaa agagctggct
     1141 ggtgtggtcg caggaggaat cacgtttcaa gaaaaaaaag agaacgtttc taacaaaagt
     1201 acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa tcttgattcg aaatagcgaa
     1261 aaagagtctt ccccaaattt gaaattaaaa aaaacttatg cggatcaaag cgatggaaga
     1321 aatgtgatta aggcgaatct tcaaaatcca aatgcttctt atatgttcgg cttgactaca
     1381 tcggttgaaa tctacaaaaa agaaacaaaa gagccttatc tttcaataaa aaaagaaaaa
     1441 atagatgtag cacccaatac aaattttgat ttacttgtcc cattagaagg aaaaaaatta
     1501 gctccgggac attatcaagc ggttgtgaag ggtgcttgga aagaaaaacg ctggaatctc
     1561 cataccgatt ttgaaataac tgatgatgca gcggaccaat tgaatgaaaa ggatattgat
     1621 ttaaaaaatc agccagaaac atttgactgg cgatggctga tacttgttgg agtcattctt
     1681 attggcttat ttgctggatt atttatttta aaagaaagaa ggaaaaagaa atgaaaaaaa
     1741 caattgcatt aagtaccgtt ctagctggaa gtctattatt atgtacacaa agtgtttttg
     1801 ctgagaataa gagctcagcc actacagaag gaacgatttc tgtaacaaaa ccagatgccg
     1861 caaatcctga agacgttgtt ccagttcctc ctgttgatcc aaatgatccc gatacgccgt
     1921 ttacacctaa cgaaccaaat cagggaacat ccggattact gacgattgac caagctccag
     1981 atcttgattt tggaacaatc aagttaggta cagcaaaaac agtatatgct gcattacaaa
     2041 acggaacgga cagtacaggg acagcaacac aagtacagaa tttcgtacaa gtaacggata
     2101 aaagcggaat ttatgccggt tggaaactat cagtcaaacg aacagaattt attggtacaa
     2161 catctgatac gaataaacta actggttcaa aggtgaaatt caaaaacgcc gtgattcgaa
     2221 atagcgcaac taatagcagt ggaacgccat ctactatttc tggtactggc gctgaaggaa
     2281 ttgagatccc agcagatcag gcagtagatc tagtaactgc aggagcaaat gaagggattg
     2341 gcacatggat ttattcatta ggagagaatg tggaacaagg gagacaatca gtggaattat
     2401 ttgttcctgt atccaattat gccgtagaca actacgtttc aacattcaca tggtcgttaa
     2461 cagatgctcc tacagaaggg aaagcgtaaa atcaaatcaa tgacttcagg gagtggagcc
     2521 gattcaaggc caactccctg aaatctgcta tgcttacgtc tcagctctta ttcatcaact
     2581 cgaagcagtt ttttggaagt catggaaacg ttcctttgtc ttttcaaaga tctttcgacc
     2641 aacgagtaag gagtcaccat tgaaaaattc taacacataa tgagcagaat caattttctg
     2701 caattgcgat aagttgatac agtaagattt taatagtaaa aagtaagcat acttttccaa
     2761 ctctgtcttc aattgtttta aagaagtatt cactaattta tcttctgtag ttgttttgac
     2821 cataacttgg tttttcaaac ctttgatact ttcaatatat aaaatttccc gaggatacag
     2881 gacgagactt ccaccttttt gatcaataaa gattttatcg acatttacga tttgctgttc
     2941 tttccaattg atctgttcta gcacttgacg aatttgttca gataaattcc tgtcttttct
     3001 gatatatcca atcggaagga ttttccgatt gatgatctct actgcaaaat tcgtataagc
     3061 ggtgaaaaaa acaatattac tatgtgcatt ttttttacgt atttgttcgg cgacatcaat
     3121 acctgtgagt gattgcttca agtcgatatc gataaagaaa acatcgtgat tatcaatagt
     3181 acccaactgg atcaactgat tgatttgaga gatagaagtg agctgcacga tattctcgat
     3241 atgatgattg accaattctt tgatgatcca ttcgctgtga aactggttat cttctatgat
     3301 aaaatacttt ttcatatgca ggctcctatt aaaaaaatat gtgtacattt tgaaacaaaa
     3361 caatggagga ataagatggt cgcaacagtg acactttatt tgacctttac tttgatttta
     3421 gatttaggta tctatttatt cttttcgaaa caactgctag taaaaaacag ctttggtttt
     3481 atagctttga tggtggtgac acttttattt cctatcccta aagtgagctt tccttaccta
     3541 gttggcttat tattgatgtt tcttttttat tttactacga aaaacctgtt tatcagctgt
     3601 ttgatcagta atcttttttt tgctatcact ggactcagct tttttctggt ttttgatctg
     3661 atggattatt taggaaagag acatcttgga acagcaagtt tttttgcact acttatcgca
     3721 tttattttct atctattagt aaaaagtata gacaaacaga cgagatttct caacttgctt
     3781 tttacttatc caactgaaca ctattttgga ggtatttgta ttttattaag ctggggcgtg
     3841 atttacttgg tattgggaag tgttcacttc cgctcatcta attatctgct tctatctctt
     3901 ttgtcgatag tcatcagttt tttttctttg tttttttcca ttatgttgat cagtttccaa
     3961 gtaaaagaaa gacaacatca ggaatcgctt caactgtatc aaataaatga agaatattac
     4021 catcatctag aggaattcaa acatgattat aaaagtctac tgttttcatt aaaaattact
     4081 cttcaggaaa ataaaaaaga aacgttggaa tttttagaaa tcttagaaga tcattctgca
     4141 gctatttttg aaaaaccgca aatacaacag ctaacaaata ttgtatcccc agctgtcagg
     4201 ggtgtctttt taaaatttct agaaaaagca gaatcggaga aaatcccgat ccaagtagtt
     4261 attcctgtcg aagtgaaaac aatcccgatt gatctggttg tttttattcg ctgtttatct
     4321 atttttattt ctaacgcatt cgatcatcgt gtagccgatc agagtccgat tacgatcaat
     4381 ctgctggaag aaaaagaggg attacgtttt agtatagcga acaaggcaaa tccgacgaat
     4441 ttgacgctat cggagatggt caaaagagga aaaacctcta aaaaaaatgg cgggttaggt
     4501 ctatacagtg cgaaaaaaat gttggatgtt tacgaaaatg cagaactaaa aatcgaattt
     4561 tcaaaacaga atcactattt ttttgtagaa atgtatctgg catctagaaa gagcaaatca
     4621 gcctactgac ttactgtcaa aggataagct tgaggaaagt ctacacggag atagagacaa
     4681 caacacagca tattcaaaag aaacagaagc gtgatgaccg ttgtcaccct tctgtttctt
     4741 ttgctatgta gttaaacaaa gtagttgaca cttccgctac ttagtattag tacgaaagta
     4801 tacttcatga tactaagtag ataagaagtg aaaagcggaa cccacgatcg tcaaaatgct
     4861 aactgctttg cttacaccag ataaagggaa gagcaatatc gcttatttta ggcgtggaga
     4921 agcaagaaaa tccctacgat cacacaaaaa atgctgaccc agcagttaag agagttagaa
     4981 caagatggca ttgtccatcg aaaagtctat aaccaagtcc cgcctaaagt agagtactcg
     5041 ctcaccgaag aaggaaaatc attaagagag attctagtag cgatgtctgt ttggggagaa
     5101 gaacgtatcg aaaaacaaaa agcctcaggg gaagaagtca cattactgca tacacatggt
     5161 ggctacctaa attactgatt tctatgacat ttattttaaa taaaagaaaa caggagtttt
     5221 tttaaaagca tcatctattt tttatgtaaa aaaatggtat gattaatcaa gtaagaaatg
     5281 attggaagaa ggcaagcaaa tgaatagcat ggaaaaagtt aaccagttta gagatgaacg
     5341 taattggcga caatttcata acgaaaaaga tttggctatt tctatttctt tggaagcatc
     5401 tgaattgtta gaactttttc aatggaaaac gccggaagaa gtaaaagaaa cacaattaga
     5461 acgaatcaaa gaagaactgg cggatgtatt gatctattca tacatgatgg cagacaacct
     5521 tcagctggat ctagatgaga tcattgaaga aaaattgatc aaaaataatt tgaaatatcc
     5581 tgtagaaaaa agcgcaggtg tacgaaaaaa atacacagag ttttagaaat gtgtcattcg
     5641 gctgttggaa agagcaaaag aaaaacagta aaatgagtct ggagggagag aaaatgaaaa
     5701 aaaaaggaaa agcaaaaaga tggctagtca atatcctctt gttcctcttg ttattagtag
     5761 gactagcttt gattttcaat gaacagatca aggattatct tgtcagagag acaggagata
     5821 aatatgctat agcgaatgta acaaaagaag atctgaaaaa gaataacgat aaagatgtca
     5881 gttttgactt tgatgcggta gaaccaatga cgacagaagg ggtcatgagg tcgcagatga
     5941 gaggaacaga tctaccagtg attgctagta tcgcagtccc ttcagtttca atcaatttac
     6001 cgattttcaa aggcttggat aatacgtcac tactttacgg agcaggaaca ttatctccag
     6061 atcaagaaat gggaaaagga aattatgcct tagcaagcca tcgtgcaacg aaccctgaat
     6121 tgttgtttac accgctggaa aatttagaga tgggtgccaa gatctatttg acagatttag
     6181 aaaatgtgta tacatacaaa acatttttta aagaaaaagt tgctcctaca gatacccaat
     6241 tactgaatga agtcgaggaa aaagaaattg tcacactaat tacttgtggc gacatggacg
     6301 ccgtcacgcg actagttgta caaggagaac tggaaagtgt tacaccgata aaagatgcga
     6361 cagatgatat gagatctgct ttcaatctag agacaaaaac attttaggat actgggacat
     6421 aagtcgttac agattaaaaa cagccaaagg atatgaatcg ataaggaaaa accaatttgc
     6481 tgaaaaaaag aggaacgcaa agaggtgcat ctctgatttg gttgttttct agatagactt
     6541 gtgttccagt ttttttattt tttatatgaa aaggcttgca taaataaaaa ttcgatggta
     6601 tgatgatttt gaataagaaa tggattgtta ctgtacggca ggcaaaacca gaattcagct
     6661 aagaaacagt aggcgaattc taggtttttt tatttttctt gagagggcga agcaaaatga
     6721 aaattgataa aattttgaat aacaatgtcg tcatttcgaa aaatggattc ggggaagaag
     6781 tcgtttgtat gggaaaagga ctagcttttc aaaaaaaaga ttggggacga gatttcgcca
     6841 gaagcggtcc aaaaagaatt tattttaaga gattcttttg ctaaaaacca attccagcag
     6901 ctgatggctg atattccggc agaggagatc gaatgggtca aacaagtagt cgaactggct
     6961 gaagataaac taaaagttga attttcgcct aatatttatc tgacattgac cgatcatctt
     7021 cattatgcga tcacacgagc aaaagaaaat atccaactgc ctaatccatt attatttgaa
     7081 acaaaaaaat tttatccgaa agaatatcaa gcagcgaaag aggctctttt gttcgtgaaa
     7141 caaaagacgg gtgtggaact ttctgaggat gaagccggtt ttattgcctt tcattttgtg
     7201 aatagccaac aaggaaacga agacatgcaa gtgacgatga ccgctaccac aatggtaaga
     7261 gatatactga gtatcatcag taaattcttc ggcatcgtgt tcgatgaaga atctttgaat
     7321 tatcagcgta tcattactca tctgcagttt tttgcccagc gttatctcaa aggggaagga
     7381 tctgacgagc aagatgaatt tctctacgca ttagttcaag gaaagtatcc aaaagcgttt
     7441 cattgtgtcg aacggatcaa tgagtatctt cgacaaacgc aaaaacagga aatggggatc
     7501 gcagagcaga tttatttaac gattcatatt caaagagtag taagtgagaa aaaaagcatc
     7561 caatcataag gattgttact gtatggcagg caaaacctgg attgaaaaaa taggttcacg
     7621 aatgtttgtg actgtttttt tcaatctggg tttttatttt ttaaataaaa aagaaagaag
     7681 gaaaaaacaa tggataatca agcagttggt cgtcgtgtat gggaagcggt aggaggacaa
     7741 aagaatgtca aaagtttagt acattgtgct acgcggttgc gattcagatt aaaagatgaa
     7801 tctttagcag atacgcaaaa gttaaaagaa gacccggacg tgatccaagt cgtacaaagt
     7861 ggcggtcagt atcaagtagt gattggcagt aatgtagcag atgtttatca atcgatcgtg
     7921 gatgaggaag gtcttgcagt ttcggaggat ggaaaagaag agtccaaaaa tattttgaat
     7981 cgtctgatcg atattatctc tagtatattt acaccttttc taggggcgat ggcagccgcc
     8041 ggggtattga aaggattctt atcattggca acggtgatgg gctggctgac agctgatagc
     8101 ggtacttatc aaatattgtt tgctgcagca gatggtgtct ttaccttctt acctgtcatg
     8161 cttgctttta cagcagctaa aaagttcaaa gccaaccagt ttttagcagt agcaattgct
     8221 atggcacttg tgtatccagc tatcacagca gttgctggtg cgggagaagc tatcagtttc
     8281 tttgggatcc cagtgattct ttcaccatct ggttatactt cttctgtcat accaatcatt
     8341 ctagccgtat gggttcaaag taaattagag ccattcgtaa aaaaagtgat tccgcaattt
     8401 ctgcaaatga ttttagttcc gcttgtcgtt ttagtagtga tggttccatt aacatttctt
     8461 gctttaggac caatcgggac ggttgccggt aatgctttag gcggtctctt caactcgatt
     8521 tatggattta gtccaatcgt agcaggtcta atcatgggaa gtttatggca agtattcgtg
     8581 atgttcggca tgcattgggg attcgtaccg atcatgtttt tgaatatcga acaatatggg
     8641 tttgatgtat tgatgccaat gcttcttcca gccatcttgg cacaaggtgg ggctgcttta
     8701 gctgtagcat tgcgaacaaa agatacgaaa cttcgagcat tagggatttc ttctacagtg
     8761 acttcgttat ttggaatcac tgaaccaact gtgtatggtg tgacattacc tttgaagaaa
     8821 ccattcattg ccgcttgtat ttctggaggt attggtggag ctattatcgg attttccgga
     8881 gtaaaagctt tctccagcag tttggtcagc ttattgacga ttccgacttt cattaatacg
     8941 gtggatggtg tagagtcaaa tgtgacagta gcagtaatag ctacaggaat tgcttttgtt
     9001 cttgcattcg taggtacatt gattttagga tttgacgaac aaacacaaga caatcaatta
     9061 gaaaacaaac atgcgaatgc aggagaaccg attacttcag cacggcatac cttaaaaagt
     9121 cctttaacgg gtaaagtact gccattgagt gaagtaccag accaagtttt ttcttctggc
     9181 gtgatgggaa aaggtatcgc tattgatcca gaagtaggag aactagttgc acctgcagac
     9241 ggagagatca cgactatttt tccaactgga catgctgtag ggatcacaac gactgacgga
     9301 gcggagattt tgattcatat cgggatggat acagtagaat taaatgggaa tggctttgag
     9361 atcttagtca aacaagggga tctcgtcaaa gcaggagatt tattgattcg ttttgacatc
     9421 gaagcaatta gagcagctgg ctatagcgta atcactccag ttgtcattac gaatactgat
     9481 gcatttgccg atatacttga attagaccaa aaagagatta ttgcaaacga agatgttctg
     9541 gcaattgtaa aataaggagg aaatcaggat gcaatcacgt ttttcagaaa aatttttatg
     9601 gggtggagca actgctgcca aatcaattag aaggggctta tgatcgagac ggaaaaggac
     9661 tatccgttgc agatgctatg cctggaggta agcagcgctt tgcgattatc ggcagtgaag
     9721 aatttgactg gacgattgat caagaaaaat atatttaccc gaatcatcga ggaatcgatc
     9781 attacgatcg tttcaaagaa gatcttgcct tgtttgcgga aatgggattc aaatgttatc
     9841 gtttttcgat tgcctggaca cgaatttttc caaatggaga cgaaggtact cctaacgaag
     9901 cgggattaga attttatgat cagctgattg atgagtgctt aaaatatgat attgaacctg
     9961 tgatcacgat ttctcattac gaaatgcctc ttcatttggc aaaagaatac ggtggctgga
    10021 aaaatcgaaa actgatcgac ttctatgaac gatttgctca gacagtatta gagagataca
    10081 gcagtaaagt caaatattgg atgactttca atgaaatcaa ttcggccttt cactttccag
    10141 ctttaagtca aggattagta aaaagcaatg gagcaggaga gtaccaaaat attttccaag
    10201 catggcataa tcaattcgtt gcaagtagca aagccgtaaa aatcggccac gaattgcgtt
    10261 cggatatcca aatcggctgc atgattatct atgcaacaac ttacagcatc gatgccaatc
    10321 cagtcaatca ggcagcaaca atgatccaaa accaagaatt caattttttc tgcacggatg
    10381 tccaagtacg tggagaatac cctgcataca cagcgcgtac ccataaaaaa tatggagtcg
    10441 atccggaaaa attagagcaa acggaaaaag acttccagtt gctaaaagaa tatccagtgg
    10501 actacatcgg atttagttat tatatgtcta cggcaatcaa tgagactgat ccacaagcag
    10561 caacttcaga agggaacttg ctgggaggag taaaaaatcc gtttcttgag gcaagtgaat
    10621 ggggctggca gatcgatcca gaagggctga gaatcgcatt gaatgaatta tacaatcgtt
    10681 accagaaacc attattcatt gttgaaaacg gtcttggagc cattgatcat atggaagagg
    10741 atggcacgat tattgatgat tatcggatcg attatttacg tcgtcatatc gaagcgatgg
    10801 ctaatgcagt agcagacggt gtagatttga tgggctatac gccttgggga tgtatcgatc
    10861 ttgtcagtgc atcaacagga gaaatgagta aacgttacgg ctttatctat gtggatctag
    10921 acgatgaagg aaatgggaca ctgaatcgat caaagaaaaa atcttttgat tggtataaag
    10981 aagtaattgc ctcaaatggc gtaaacttgt aattaaagca gctgaatgaa aaagaacaca
    11041 cttctcacat gtgatttgtg ttcttttttt attttttatg tgctattctt ttagtaatag
    11101 taaagcaaag ggtgacgtta tgaaaaggac acgtgtacta tacttggaag tcgcagataa
    11161 aattaaagaa gatattttct caggaaaata tccagtcggt tcgatgttgc cgacagaaag
    11221 tgaattagaa gaactgtttc aagtcagcaa aatcactgtc cgaaaggcaa tcgaactgct
    11281 ggcatcagat gaatatgtcg caaagaaaag tggtcgtggg acgacggttc taagcaatcg
    11341 tccttacaat cgattatcca aagcggcaag ctttacgcaa atcttggaga actcccgata
    11401 tgaagtccgg aaagaaacgc tggatttgca attggtccat ataaagaagg aagatccttt
    11461 gttttccttt ttcggtgagc aagctgtttg ttttaagcgc ttatattatt tgaacaatca
    11521 gccttatatt tattttgttc attatcttcc acaagaaatg atgcaattca gcagagaaga
    11581 atttggcaaa gaatctttgt atcgtttgct gacgaaaaaa ggatatacta ttgactcatt
    11641 ttctgatgat ttctcggcag tatttttaac agaagaagaa caaggaattc tgaaaacaga
    11701 cgcacagatc ggcatcaaac ggatacggaa atcgtctacg caaattgggg aaatgattga
    11761 atattcggag gcggtctacc atacggagct gcatccttac catattgatt atgaaacata
    11821 aataaaaaaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
    11881 aaatagcttc tcatattttt cgtttggtta ggcttattac cgcagataca cggctgtttc
    11941 tggattacca tttattctgg ataagggggt gtggcacgat tttcagaagg aatctatgaa
    12001 ccaatcactg tattgatttt agcttcgatg gtaaccccat tcggcttgac gatcgcttat
    12061 ttcttaggga aaatcattcg taaaaatata ctcaatcgtc aagaaatcga tacattgaag
    12121 accgcttttc ctatggggat ctgtcagatc acagagggat gtttccctat cgtattgaac
    12181 gacttggcac gcaatgtcat agcaacggga gtcggtggga cagtaggtgg cggattaagc
    12241 atgttttggg gcgcagatag ccgtattccg gcatcgggaa tgtttgcagt cgctacgatg
    12301 accagaccat gggcattcat tggtgcattg ttagctggtt cttttgtcac aggaataacc
    12361 attttattat tgaaaaaacc agtagatccg aatgcagaaa tcatccaaga agaaaaagag
    12421 gaagaagaca tatcttggga tgatttgaca attagttagg agaggcagaa tggattacaa
    12481 gaaaaaagta caatagatgt accttgagac aggaatggta tgaaagaatc agagttagaa
    12541 agaatcgaat atacagattt tggtttagga gcgatcgaaa ctgtccagag catcggcacc
    12601 ctccttgaaa tgacgatgaa ggaaaaatga gaacctttcg ttgtcgaaag ggtctggtat
    12661 accttatgtc gaaggaaagg aaacaaaaaa actttcctgt actattccaa aaaggaaaga
    12721 aagttattat acagtaaaaa aagaaattat tcttcatgct agagatcagt acacgttcga
    12781 gcccaatatc aaacactagt tccaggcagg cgcaacaggt gcggttatca ctgaattttc
    12841 ttccagcagt gacgattcaa gtgatatttt tacaaatcca gctatttatc gttaacaaaa
    12901 agatttttag gagaagctga tgaacaaaaa tgatcaaatt ttgatcgttt agttcgtcgg
    12961 cttttttatt tagagaaaca tcatttatca gtgaaacata acacgagaac aaaatgattt
    13021 tttcgttaat aaaagaaaca agtatttgtt gtttaaaata atacacgtta ttggcatata
    13081 ggacgaaatc ccagtatctt ctataagtaa gtcatttcat atcgatgaaa acacgttatt
    13141 tatttttgtt ttatctataa aatggctgaa aaatgatttt tttctaaata ctatatatag
    13201 tggcgtctat cctatatacc actaaaaaac tacaaaatct agtcttttat tttaaaaaaa
    13261 cacgtgtaga atgaagatag acttttgagt tagggagtgt ttttttatga aacttactgt
    13321 tttttgcggt tcccgttttg gtaataaaga aagttacaaa tttattgcgc aaacgttagg
    13381 aaaatacatg gcacaagaag aaatcgaact ggtttacggt ggttcatact ccgggattat
    13441 gggagtgatt agtaaaacag tactggaaaa taacggaaaa gtcacaggta tctatccgaa
    13501 tgggcttttt gaaactgaat tgcctggaaa agatgttact acatttattc ctacagaaac
    13561 gatcgatgaa agaaaggtat tattatttga aaagggagat gctgtcctcg ttttccctgg
    13621 aggtctaggt acattggagg aattctcaca acttctttct tggatagcaa tcggtctgac
    13681 gcccgacaag ccaatcggaa tattgaatat tggcggttat tacagcggct tgcaacaatt
    13741 attagaaact tttgcaaaag aggattttat ggataaaaaa tggttggatc aagtaatctt
    13801 ttccaacaat ccattggaac tggtcaatga attacgagca gtatctatta aaaatcagtt
    13861 attattgaag gaggcaaaat aatgagtcaa atatatttag caggaccatt tttttcagag
    13921 gagcaaatcg atcgagtaag tcgtatcgaa aaagcgttgg aggaaaacaa aacagtcaca
    13981 agtttctact ctccacgtca tcaccaagaa agcaattatg aactattcag cgctggatgg
    14041 gcgcaggaag tatacgaaaa agatatggaa gaactgacaa atgcagaatt tgttgttgct
    14101 atccttgact ttgagcatca aacaatcgat ccaggaacgg cttacgaatt aggtgtggca
    14161 actatgttga aaaaaccgat gatcattgta caagaagaga cggtacctac gaacttgatg
    14221 attacacaaa gtctccatac gtatttaaaa tcagaccaag ctgtacgtga atatgatttt
    14281 gaaacattgc ctgttgaaac ttacgtaggc gagtacttat agaagaggaa gggttgtatg
    14341 tcagtgattt tgatttcagg aacgattggt gcaggtaaga gcagtttgac ggatatgctt
    14401 gcaaaagaga ttgattctaa accattttat gaaaatgtcg aagataacga agtattgccg
    14461 ctcttttatt caaatcctga gcagtacgct tttttattgc aaatcttctt tttgaacaag
    14521 cgatttttag caatgaaaaa tgctttagtg aacgatgaca atgtgctaga ccgatccatc
    14581 tatgaagata gcctgttgtt ccatctaaac gctgatttag gtcgggtaac ggatatcgaa
    14641 gtacagcagt acgatagctt attagatacg atgctaaacg agttagatga tgttgctcca
    14701 aaaaaacggc ctgatttgat ggtccatatc aaagtttctt tggacacgat gctagaacgt
    14761 atcaaaaaac gtgggcgtga ctatgaacag ctagaaagcg atgagacgtt atatacctat
    14821 tatgaaacgt tgaacacccg ctataatcaa tggtatgaag attttgatgt atgtcctaaa
    14881 atacaagtgg atggggataa attcgatttt gtagaaaatc cagaagatgc aaaaaaggtg
    14941 atccgtcaga tccaagaaaa attagctgaa ttggaaggaa agcctgctgg aaaatatttt
    15001 acggataacc agcatgcaaa aggtcaagaa ttaggctaaa aataaaaaac caaacgacgg
    15061 gaaagaaaaa tt
//
LOCUS       DAZKOW010000070        14943 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000070 DAZKOW010000000
VERSION     DAZKOW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.070"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(510..1076)
                     /locus_tag="ACG912_002027"
     CDS             complement(510..1076)
                     /locus_tag="ACG912_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0513332.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1076..2032)
                     /locus_tag="ACG912_002028"
     CDS             complement(1076..2032)
                     /locus_tag="ACG912_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFQ0513333.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2032..2688)
                     /locus_tag="ACG912_002029"
     CDS             complement(2032..2688)
                     /locus_tag="ACG912_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0513334.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3058..5076
                     /locus_tag="ACG912_002030"
     CDS             3058..5076
                     /locus_tag="ACG912_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0513335.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5146..5352
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5503..7917
                     /gene="leuS"
                     /locus_tag="ACG912_002031"
     CDS             5503..7917
                     /gene="leuS"
                     /locus_tag="ACG912_002031"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFQ0513336.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8265..9818
                     /locus_tag="ACG912_002032"
     CDS             8265..9818
                     /locus_tag="ACG912_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFQ0513337.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10199..11983)
                     /locus_tag="ACG912_002033"
     CDS             complement(10199..11983)
                     /locus_tag="ACG912_002033"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513338.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11998..13719)
                     /locus_tag="ACG912_002034"
     CDS             complement(11998..13719)
                     /locus_tag="ACG912_002034"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513339.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13819..14580
                     /locus_tag="ACG912_002035"
     CDS             13819..14580
                     /locus_tag="ACG912_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0513340.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4861 a         2745 c         2956 g         4381 t
ORIGIN      
        1 tcttttttac tgctttgatc ataaaagtct gcatattcat cactgatatt ttttctaata
       61 aattcttcca tcataaaaat cacctcaaaa cgattatacg aacttacgtt cttgttgtaa
      121 agcgatattt gaggggcaaa aaaggggcat aatttgactg atattaagtt agctattgat
      181 atatgaatga catacaaaaa tataaaatct tttaaaaatg cttatatatc aatgtttttg
      241 ccattctttt atatatctgt tatatgtcaa aaaaatgtgt tcaatttcaa tttgtatttg
      301 aataaagagg ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
      361 agcttctcat gtttttgatt tggttggact tattaccgca gatataagct tttgagcacc
      421 gtgaataaag aggttatgaa atcaacgctc taacctgagt attagagaaa aaaacgcaaa
      481 acatatcatt tatgttttgc gttttttatt tatctttttt tctcaataca atataaaata
      541 ggcggctggt tcttttggtt gataaaccga taattcagca cattatatgt ttcctgtggc
      601 agtttggaac aaaaagaatc aaccatatcc agttcttttt ctccaccttc atgaccatag
      661 tagacgacaa ggatcaatcg tccgcgaggc acaagtcgtt tcaatatttc ttccatagct
      721 gtgcgtgtag tctctggcat agtgatgatt gctttatcac tttttgggag ataccctaga
      781 ttgaaaatcc ctgctttgag attttgttgt tcagctatca cttctcctaa cgtttcgtgg
      841 ccttgtagaa acaatgagac acgttcttct aactgacgct gttctagcct ttcacgagta
      901 gcatcgattg cttgttgctg gatatcaaaa ctatagacat gaccagtttt tccgatgtgt
      961 tctgctagaa aaaccgtatc gtggccattg cccatcgttg catctacgac gtgatcccct
     1021 ggttgcaaga tttcttgcag taatgtatgg ctgaaatgca aagcagtttg caacatcaga
     1081 cgctcacctt tttccgtaca tcatattttc cttggaagct atctctgcga agaagttctt
     1141 catcaattgc attcaagact tcccattttt tcaagctcca cattggtcca atcaaggaat
     1201 cccacggcgc atcccctgtc aaacgatgga tgatgatctc ttttggtatc atttccagct
     1261 ggtcgcaaat gacattgaca tagtcaggac ggctcattaa ttgaaggcga ccttcatgat
     1321 agtcccgcaa cattctcgtt ttgcgcatca gatgcatcaa atggagcttg attccttgga
     1381 tatctgaatc taaaatggtg cgtctgacat tttctttcat catttctaaa ctttcgcctg
     1441 gtaatccgtt gatcaaatgc gtacaaacac ggatattatg tttgcgtaat ttagccacgc
     1501 catctaaata agtttgataa tcatgtgctc ggttgatcag ttttgaagtt tcttcaaaag
     1561 ttgtttgcaa tccaagttct acccataaat acatccgctc gttcagttca gcaaggtaat
     1621 caactacttc atctggcaga caatccggtc gtgtaccgac tgataatcct acgacacctg
     1681 gtaaattgac tacttgctca aatcgttctt tgattatttc aactggcgca tgggtgtttg
     1741 taaaattttg gaaataaacg atgtattgtt ggacatgagg ccatttttga tgcatcatgt
     1801 cgatttcttt ctggaattgg attggaagag ggtcttcagg tgcaacgatc atgtctcccg
     1861 aaccagatac actgcaaaaa gtacaaccgc catgggcaac agtcccatct cgattcggac
     1921 agtcaaatcc tccatcgatc ggcaccttga aaattttccc gccaaactca tttcgtaaag
     1981 cgtaattcca agaatgatat cttttatttc cttctgaata aggaaaagtc attaaaagtc
     2041 cttctttctg taagagttat ctatttgttt tcttttttct ctatacactg gataagtcag
     2101 gcacagccaa gaaagactga gtaagtaacc gccgatgata tcagtaggaa aatgaacgcc
     2161 cagataaatg cggcttatcc cgacacttac tataataaac atcatcagca attgaagcaa
     2221 tgtgcgtaag gttctttttt gaacaaaagc tggaagcaga aatagtaaag aaccgtaaag
     2281 aatcatactg cctgctgcat gtccactagg gaaactgtaa cttgtttcaa ctactaagtg
     2341 cgccaatgaa ggacgctctc gcatgaaaaa tagtttgatc accgggttga tgattccagc
     2401 aattacaata aatccagaaa cgaaccaaaa tgtttctgcc ctttttttct tataccacat
     2461 ccataaagcc gcacaagcaa ataaaatgat gacggtaaga ggattaccga atttagtcat
     2521 aaagagttgg tagctgttca gatcaggata agaagctctg acaatgcctg taaaccactg
     2581 atcgaaacca gaaagccacg acagatggaa ctttacgatc cagcccaaaa tgaaaaacag
     2641 gacaagaaag aagctaccag ccacctgaaa tgatgtttta tatttcatac aattcctcct
     2701 ttgaagcaat gacttattat agcatttttg gtccagcgaa aaaaacggta aagagataaa
     2761 accaatgtat gattcctctt tgaataacca atattcttat aaaaaaaata actgtaatcg
     2821 gaaagacatt ttttggtaat gcaaatgaaa tgttagggaa ataaaatcag tgtttttcga
     2881 aatcctttta aacaagaacg tagacgtagg aagaacttga aaatgaacag aagttgttca
     2941 ttacggtaaa ttacaatatg attacaaaaa acaacaaagc tcttaaataa cttttaataa
     3001 atattaagag aagttataat tattaataat agataaattg aagttggagg atagaagatg
     3061 gaaaacattg caagaaaaga acggagacgt ttagaacaga ccaaacgttt tcgaaaagca
     3121 aaacgagggg cagcaatcgt gggaactgcg atggtaggct gttctgtcgc tgccccatta
     3181 gtacagccgg ttcctgttca agcggatgaa acacctgttc agttcggcgc aagaatcaat
     3241 acagcggcat ttatcacaga aattgctact tatgctcaac caatcgctca agccaacgat
     3301 ctatatgcat cagtgatgat cgcacaagca gttgtagaaa gcggttgggg cagtagtgcg
     3361 ttatctcaag caccttatta taatcttttt gggataaaag gcagctatca aggacaaacc
     3421 gtctacatgg atacgcttga atttttaaat aatcaatggg taaataaaaa ggagcctttc
     3481 cgacaatatc cttcttttgc tgaatctttc aatgacaatg cctatgtact tagaaatact
     3541 tcatttggca acggttatta ctatgccggt acttggaaga gcaacacgaa atcctataca
     3601 gatgcgacag cttgtttgac agggcgttat gcgactgatc ctggttacgc aggcaaatta
     3661 aataatatca tcgcaactta cggattgacc caatatgata caccagctag cggaaacgca
     3721 ggcggtggag cgactacagg tggcaataat actggaagtg caggtagtac tggcggttca
     3781 agcaattctg gtaatacaaa ctcaggttca gcaacttcga ccacttatac tgtccaatct
     3841 ggtgattctg tttggggaat ctcaaataaa ttcggtatca caatggatca attgatccaa
     3901 tggaacaaca tccaaaacaa tttcatttat ccaggacaaa aactgacaat caaaggtagc
     3961 caggaaaatg gctcttcgac gaataattcc ggtaacaata ccaattcttc aggaaatgct
     4021 ggtacatcaa acggaggaca aacaactggt gcgaaataca ctgttcaatc tggtgattct
     4081 gtttggaaga tctcaaatga tcacggaatt acgatggctc aattgattga atggaacaat
     4141 atcaagaaca atttcgttta tccaggacaa cagctaatcg tatcaaatgg cggttcttct
     4201 aatgctggag caaatactgg ctcaaatact gggaatacaa gtggctctag ttcgtccagc
     4261 tcaagttctt ctaatacagc ttcaggaacg aaatatacag ttaaagccgg agactctgtt
     4321 tggagcgtct caaataaata cggaattacg atggctcagc taatccaatg gaacaatatc
     4381 caaaataact ttatctaccc aggacaacaa ctgactgttt caaacagtgg atcaacaaac
     4441 agcacgtcag gtaatacagg ttcgtcaacc aatacaaata acagcagttc agttaatact
     4501 ggcagcagtt cgtctcaagg cacttatacc gtgaaagcag gtgaatctgt ttggagcgtg
     4561 gcaaataaaa acggcatcac gatggatcag ttaatcgaat ggaataatat caaaaacaac
     4621 ttcatttatc caggacagca attgatcgtt aaaggtggaa cttcggcaaa tacaaataca
     4681 ggttcaacta cttctgcagc gaaaccaaat acaccgaata cgtctgctac gacaagtaca
     4741 tcatccactg gtaatacgat gtataccgtg aaagcgggcg agtcagtttg gagcgtagcg
     4801 aacaaacatc atatcacgat ggatcagtta atcgaatgga ataatatcaa aaataatttc
     4861 atctatccag gacagcaagt gattgtgaag aaaggttcat ctcaatcaac aaaccaacaa
     4921 tcagttactg gttcgaaaac ctatactgtg aaatctggag aatctgtttg gagtgttgct
     4981 gacagtcatg gaattacaat ggctcaacta atcgaatgga acaatattaa gaacaacttt
     5041 atttatccag gtcaaacgtt gattgtagct aaataaaata gctgttcgaa gaaataacga
     5101 ttgaattcca caaaaatatt tgttactata aggctactta aatatgacaa cacttgaaaa
     5161 ggaagtagta aaaagactga agtttcagag agcgcatggc tggtgagaat gtgcacggat
     5221 gcttttgaac tcgcctttga gttgcccttg ctgaataccc gagtaagcaa gcggcggtga
     5281 cggccgttac aacgttcgag gccttttcac taaggtaaaa ttaggtggta ccgcgtagat
     5341 tacgtcctat aaaaagagca atctttttat aggatttttt tatttctttt gtgaagtttg
     5401 agaaaacaaa gcaaaaggaa ataaaaataa acagattctg ccggtttttt cgttatattt
     5461 ataatagaaa caagtgtttt caatagaaca ggaggacagc aaatgagtta caatcataaa
     5521 gaaattgaga aaaaatggca aaaatactgg gcaaaaacga atactttcaa cacccatgat
     5581 gatcctgaaa agccaaaatt ttacgcactg gatatgtttc cctatccatc cggtcaaggg
     5641 ctacatgtag ggcatcctga aggttatacg gcaacggata tcctttcacg tgtgaaaaga
     5701 agtcaaggat acaacgtgct gcatccaatg gggtgggatg cattcggtct gcctgctgaa
     5761 caatatgcgc tagatacagg aaacgatcca gctgagttta cccaaaaaaa tatcgaaaca
     5821 ttccgacgcc aaatcaattc tttaggattt agttatgact ggaatcgcga agtgaataca
     5881 actgatccgg agtattataa atggacacaa tggatcttta cgaagcttta tgaaaaaggt
     5941 ctagcttatg aagcagaagt agcggtaaac tgggtacctg aattaggaac agttatttca
     6001 aatgaagaag tgatcgatgg gaaaagtgaa cgtggtggct atgatgtagt cagaaaacca
     6061 atgcgccaat ggatgctgaa aatcactgct tatgctgatc gcttattgga tgatctagaa
     6121 ttagttgatt ggccagaaag cattaaagaa atgcaaagaa attggatcgg tcgttcagta
     6181 ggagcaaata ttgaatttaa agtggctggt acggataaat cctataccgt atttacgaca
     6241 cgaccagata ctctattcgg tgcaacttac tcggttcttg cacctgaact agatcttgtt
     6301 agagaaatta cgacccctga acaaaaagct gctgtagaag cttatatcga agagacagct
     6361 aaaaaatcag atctaaagag aacggatctt gcaaaagaaa aaacaggagt atttactggt
     6421 gcttatgcaa tcaatccagt taatggcaag gaaatcccaa tctggatcgc cgattatgta
     6481 ttatcttcat acggcacagg agccatcatg gctgttccag cacatgatga acgtgacttt
     6541 gaattcgctc aaactttcgg actagaaatt cttccagtta tcgaaggtgg agatgttgaa
     6601 aaagctgctt acacagaaga tggtacgcac atcaactctg aatttctaga cggcatgaac
     6661 aaacaagaag caatcgataa aatgaatgaa tggctagaag aaaatggcgt agggaaaaaa
     6721 gaagtcagct atcgcttacg tgactggcta ttctcccgtc aacgttactg gggtgaacca
     6781 atcccaatca tccattggga ggacggaaca gtcacagctg tcccagaaga agagctacca
     6841 ttacggttgc ctaagacaaa aaacatcaag ccaagcggta ctggagaatc tccattagca
     6901 aatatcgagg agtgggtcaa tgtcgttgat cctgtaacag gtaaaaaagg ccgtcgcgaa
     6961 acgaatacaa tgccacaatg ggcaggaagc tcatggtatt acctgcgtta tatcgaccct
     7021 cataacaaaa aagaactagc agattatgaa aagttgaagc gttggctacc agttgacatc
     7081 tacattggtg gagcagaaca tgcggtactt catttactat atgcacgatt ctggcataaa
     7141 ttcttatacg atatcggtgt tgttcctaca aaagaaccat tccaaaaact atacaatcaa
     7201 ggaatgatct taggcgaaaa caacgaaaaa atgtcaaaat ctcgtggtaa tgtagtcaat
     7261 cctgatgatg tggtcgaaac ttacggtgca gatacactgc gcatgtacga aatgttcatg
     7321 gggccattag atgcttcgat tgcttggagc gaaaatggct tagaaggaag ccgtaaattc
     7381 ttggatcgtg tatggcgttt gatcgttgat gagaacaata aaatgcgtga ccgtatcact
     7441 acattgaatg atggcaagct tgataaagtg tatcatcaaa cagtgaaaaa agtgacagaa
     7501 gactatgaaa atcttcattt caatacagca atctcccaat tgatggtctt tatcaatgaa
     7561 gcctataaag tggacgctct gccttatgaa tatatcgaag gatttgttca actactcgca
     7621 ccgattgctc ctcacatggg tgaagaacta tgggcaatcc ttggaaatga tggtggtatt
     7681 tcttacgcac catggccaac atacgatgaa gcagcacttg ttgaagacga agtagaagtt
     7741 gttttccaag taaacggtaa agttcgtgcg aaaagcaatg ttccccgaga cttaggaaaa
     7801 gatgagctag aaaaagcagc attagccaat gaaatagtgc aagaatatat tgaaggcaaa
     7861 acagtcagaa aagttattgc ggtaccaaac aagttagtca atatcgttgc taattaattg
     7921 gctctttgtc aataaggact gatgagttgt gcaaaatcaa atctgggcca gaatgaactt
     7981 cattctgtct cagatttttt gttatttcaa attgattaat tgattgatca aaaaggtttt
     8041 gattttcatg ttctcaaaca cgttcaatgt ctttttcttc tgaatggtct tgtaagagat
     8101 ctctgtaatc atcaaatttt tgtctattaa tcttagtatt ttttgatgta taaactacct
     8161 gaaattgaga gtacatacca aattcataaa ttttttatta gtgggactag tgattgtatg
     8221 gattttgacg cataatgaat gaagtatgag aaaaggagag acaaatggat caagaactat
     8281 ttattatcga agtattatca gctaaacaaa aaattatcga gaaagcgaaa aaaagagcga
     8341 aaagaataat gttcaagctt ttaagcttag tatttttctc tggtttgctt tttttcggag
     8401 ctgtatcgtt cctatatcgc catatgagtg cagtagtccg ttcagctgaa gggatgcaaa
     8461 aattaaataa tttgtatcac ttttcgtggg gagttggcgg aataacaatt gttgtgctta
     8521 ttgtttttct tctacttatt tggctccgtt accatcaatt gtcgcgtcat ttcgatcaat
     8581 ggcttagtca aagagcacaa cagtttttac ataataaacc aacttctcaa gatgatgact
     8641 atctttattt tgagaagact tcgacgagtc aagcttttcg gatacaaaaa agtgattgcc
     8701 agttattgct gagatcactt gatgatttac ctatctactt aggcgaaagc aaatcaaaat
     8761 tcggaatgaa cacattccat atttttttac tagaaaacga acccagatgg gaaaaaagcc
     8821 agtcttcgcc tggtggatac ctgacaacta agatgagtac attagtcact ggttttttcc
     8881 tcattctttt agctggaaca atctattatg ggagatacac tcagccaaaa atgtcttctc
     8941 atataaatgc tgcttctcac atgacaatga actcaactaa cgatacgcat atacaaaaga
     9001 caaatgaagg gttgttagct gatcctaagc aaaaggtaaa tttgacaaca caaaagataa
     9061 atgatctcaa acttgataca gagacgcact ctctctatat gacaacagat agtggtgcta
     9121 attgggtatt tgtgccgatt gattcgcaat ggttgcgttt tggcgaatac accctgacaa
     9181 cggggctgat tcctgaaggt tattggatgg acaacacata tgatatttcc gccgatttct
     9241 catggtttat ctattcttct ggggatgatg tacgtctgct gacttcaaga gataatggta
     9301 aaacatggca aacgtctatt atcacccaat cagttcagcc gatacgttat cgaaaagcgg
     9361 tattttatgg caacgatggt ctatttatct gtacatcagc cagtgggatg tctgcagaaa
     9421 ctttgtacat ttacactact atggatggcg gaatcacttg gataaaaagt ggaagtacaa
     9481 tgatcaatca gccagtacaa aatgtcagct tcgttacaac ttctttaggt tttgtttcga
     9541 cacgtgaaag tttgttttat acaaacaacg gaggacgctc attcaaagaa tcagttctgt
     9601 cgattccgga aaactatcag acagggggga tagatctttt tcaatcacca aatgaagtta
     9661 ctcaaataag cgcgaatacg ttagagacaa aattttattt gttgaaaaaa ggaccaatcg
     9721 atgcagggaa aatgtttgcc tgcctcttcc gttcaactga taacggggaa acatggcaat
     9781 tcgttcagca actatcgcaa gtagaacaag gagattaaag aagagagatt gtgagcctga
     9841 cgttcagcac cgagtcacaa gaaacaaatt caaaaaacag cttctcatat ttgttgaata
     9901 gtgtaactcg tgattcggag ttgacaagag caacaagaag cgattttgaa gtatagcttc
     9961 tcgtatttta caagatcgtg tcttattgcc gataggttgt ctcttgaaca ccgtttattc
    10021 gtaaaaaggg attgtgacaa gtcttttgtc actacttacc gaacgaaact gaaggagcta
    10081 tccaaaaagt atcattagta ttattctttg tgtttctagt ggcaccacta gtacatttaa
    10141 aaaagctatc gatcatggac taatcataca tgatcgatag ctttttagat aatgtttgtt
    10201 acgctatctg aaattgacta ttataaagac tggcataaag cgttggtgat tggagtagtt
    10261 cagaatgtgt tcctttttcg acgatatcgc cattcttcat tacaaggatc atgtctgcat
    10321 tttcaatcgt tgacaaacgg tgtgcaatca caaaacttgt cctatctttt gtcacagctt
    10381 ccattgcttg ctgaatcaat gcttctgttc gtgtatctac gctagaagtt gcttcatcta
    10441 aaataacgac aggaggattc gctagaatga ctcgagcaat tgtcaataat tgttgctgtc
    10501 cttgagataa cgtgccgttt tcgctagaaa tgatcgtatc atatccgtct ggcagtgttc
    10561 gaataaagtg atcacattgt gcgatttcag ccgcttcgat aatttgttct cttgtggcat
    10621 ccattttccc gtaggcgata ttatcagcta ccgttccttc aaacagccag gtattttgta
    10681 aaaccatccc aaaagaacga cgtagttctc cacgtgaaag cttggtaata tcaagaccgt
    10741 cgaattcgat ctttccttta ttcagttcat aaaaacgcat cagcagattg actaaagtcg
    10801 tttttcctgc accagttgga ccaacgatcg ctaccgtttg ttttggcttt actgaaaaat
    10861 cgacttgatt catcaatatt ttatctgatg tgtaaccaaa ctgtacattt ttgaaagcta
    10921 tttttccttt cggttgttct aaaagtcttg catctggaag gtctggaatt tcatctgttt
    10981 catctaatat ctcaaatatc ctatcgattg ctgccattgc ggattggatc gagttgatca
    11041 cataagaagc ggtagaaatt ggttcagaaa tctgattgat gtactgaaga taagcttgta
    11101 agaagccgat cgtcacacca cctgatagaa caagcatagc accgaatacc gcacttatga
    11161 tgaaggcgag ttgattaatg aaacgaatgg ccggatagat agcacagttc ataaattgag
    11221 cacgtttaaa agcgttgtat tgctttttat ttacttcgtg tatagcagct tctgcatgag
    11281 cttgctgatt gaatgttttg accactagat taccagctaa gatttcttcc attcgattat
    11341 ttaaaacacc cagttcatac tgattctgtt cagaaatctg tttgtttttg ttcgcaatct
    11401 tagttgtaac aattgaactg ccaacgatca gcaataagac gagcaaagta agcttgcttt
    11461 cgatataaaa cagcatcaga ccagcagcag ctattgttac gacagaagta aaaaattgat
    11521 tgatacctgt caataacact tgagaaagtt gattgagacc ggtagttgtc cgactaatga
    11581 tgtcacctac ttgatgattg tcaaaaaacg acatcggtaa tgttttgaat ttttttgtga
    11641 tttcttttcg caccctcaat gtgatctttt cgcttaacgc tgccattgtc cgttcctgaa
    11701 taaatgaagt gatgctgctt aagactgaac agccgatcag caataagaca gggaacaaca
    11761 atgcttgttt gatatcactg aaagaaagct gtccaatacc tgtttttctt atttctgata
    11821 agagatcatc gatcccgatt cccatgatca agggcatggc tacgactaat aggttcccga
    11881 ataaactgca tagaaacaat ccgtaaaaat atttatgttc aggtttgatc attttccaaa
    11941 aacgtatcaa agcacgcgtg ttcaacgttt ttttcttttc catcgtatcc cttctttcta
    12001 tattccttgt gattttgcaa actcctgata agtttggcaa ttagacagta gttcttcatg
    12061 agtaccttgt ccaacgattt ttccttcatc taaaacaatg atgttatctg cttgttggat
    12121 tgtactgagg cgttgagcaa caatcacgag agttttctcc gacatgtgtt tatgaagggc
    12181 tgctcttaat gctgcatctg ttttatagtc taaagcggag aaactatcat cgaatacata
    12241 gatctctgct ggtttgatca acgctcgagc aatacatatc cgctgtttct ggccccctga
    12301 ataattagaa ccgccttgag caacaaagct gtccaatccg tcaggaaggg tgtccactag
    12361 ttcttctaat tgagcgatct tgatcgcttt agacaactct tctgttgagg catattcatt
    12421 tcccatctgc aagttgctta aaattgtccc gctgaataag aaagccttct gagggacata
    12481 actgatgtgg gagcggatct gttcttgagt catttgagta agaggaatac caccaagggt
    12541 tacttttcct atcgtagggt cactaagtct caagagaagt ttcgccaaac tactttttcc
    12601 agatccggtc ccgccaacga tcgctgttgt ttttccttta ggtattgtga aatcaatatc
    12661 ttctagaact ggatcagaga tttcagtata tttaaatgtg acatgttcaa aagcagctgc
    12721 atttgtctga tcaaaggttg gttcagctac tgttttgatc gtctcttcct caatttcgat
    12781 ctctgcttgc agtatttctt ctatccgtcg taatgaggca aacgatttag ggataatgac
    12841 caataccata gctgcgatca tcaagtaagc aagtgtcagc atagagtatt cgactacagc
    12901 agtcacgaca ccgatttgta atgctccttc tgctacaaga tttccgccaa accacaaaat
    12961 agaagcataa acgattccca tgagcaaaaa agcaattgga gtgacccaag caaatattcg
    13021 gttcactttg atcatttgat tagcatagtc agcaaagctc ttattcgttc gcttttcttc
    13081 atatgattga ttatcaaagg cacggatcat attaatccca gtaaagaatt ctctcaatgt
    13141 gatggtgatc cgatccattt ttggctggat actcaaagat aaaggtgccc ctcgtttcat
    13201 cagtcgataa acaatccata cataaaaaac aacagagatc aatgtaaccc atgttagtaa
    13261 tagagaataa cgaaacatca tgagaatggt gaaaacgcaa ataaccggtg ctggtaagat
    13321 catttggcaa aataaaacaa tcatttgctg gacattatcg acatcattcg tcattcttgt
    13381 caaaagagat ccagaactaa aacgactgat atccttcatt gacagctgtt ggaatttacg
    13441 gaaatatttc tcccgcgttt ggaatccaaa cctagcggaa gccaatgctg aaaagtaact
    13501 agaggcaact gcagcgattg ttcctagaaa cgctacaagc agcatttcaa ctccaatttt
    13561 ttttataact gaggcttcgc cacttgctat acctgtgtcg atcagctttg ctactaattt
    13621 tggtacacct aatgtaccgg cgatttctaa acagatcatc acaaaggtaa ggagagcgag
    13681 tcctttgttt ttctgtaaga atgtttttaa gatagacata ttattcctcc ttttttcctg
    13741 atacttttcg attctatacc ttcaagtaac ttgaaggtca atagaaaaaa gaataaactg
    13801 aaaaagaggt gagaaaaaat gaaaaaaatg tatacgattg gccaagtatc ggaactattt
    13861 gatttgccaa aatcaacgat ccgctattgg gatgaacaag gattaatccg ttcaagtaga
    13921 caagaagaga atgattatcg attgtttgat atcgatgata tttttatgct ttatgatatc
    13981 gttttttata gaaaattaga tataccgatc aaacagatga agaatttata tggaaaaaca
    14041 ttgacggagt tatatgagac acttgatgaa acagaaagaa gaatccataa agaactggtg
    14101 gtgatgaaac aaaaacaaaa agagataagg gagcaaaaaa aacagttgaa attgatgatc
    14161 gatacaaacg aggaagaatt tcctgtagaa gagatccctt ttgactgtat gatctcaacg
    14221 gagtttgaag atattgtgga aatcaaaaaa tttctaccta actattcaag ctttgggatg
    14281 atgagtatgc cagcttccag ttcacagacg atgtatggat tttttatcga ccctagtgaa
    14341 gtacatttat ttactcaaga cgtgatttgg gagaaaaaag atacggctgt ttatcgtcga
    14401 ttcttattga aatccgagat gaaccatgcg gaacgtaata atattttaga aatcagagaa
    14461 agaatgtatg aaaaaggatg gaaaacagga gaagtgatcg gccagtatct attgacgaat
    14521 acggatgaaa acaatatcag aactgaatat tatcatgcat ggatagaaat gaaaaaatag
    14581 gaaatatgtg tttaagataa ctcgaaaatg atggttgttt tagttataaa gaaaaatttt
    14641 ctggttcgat tacgggttta atgtggggga ttattagtcg tggatagata gaattttagt
    14701 tttttagagg aaatatgtct ttaaaaatgg aatggttgca attcttagcg atatatacga
    14761 tgtaattgtc ctcttaaaaa agtatggaaa aataattgta ttgggtgtta tgtataattc
    14821 atggtgaact gagactaatg agaagaaaaa ttcatattgt tatgagtgag attgtatcag
    14881 atgagaatga taaattaaat aggtgcataa aagcattaca cttaaaatac attgttgctt
    14941 aca
//
LOCUS       DAZKOW010000071        14549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000071 DAZKOW010000000
VERSION     DAZKOW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.071"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..816
                     /locus_tag="ACG912_002036"
     CDS             4..816
                     /locus_tag="ACG912_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFQ0513341.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACG912_002037"
     CDS             813..1613
                     /locus_tag="ACG912_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFQ0513342.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACG912_002038"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACG912_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFQ0513343.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACG912_002039"
     CDS             3110..4819
                     /locus_tag="ACG912_002039"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFQ0513344.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACG912_002040"
     CDS             5031..9389
                     /locus_tag="ACG912_002040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFQ0513345.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACG912_002041"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACG912_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFQ0513346.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACG912_002042"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACG912_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFQ0513347.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACG912_002043"
     CDS             11227..11520
                     /locus_tag="ACG912_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFQ0513348.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACG912_002044"
     CDS             11517..11828
                     /locus_tag="ACG912_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFQ0513349.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACG912_002045"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACG912_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFQ0513350.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG912_002046"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACG912_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFQ0513351.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4959 a         2583 c         3238 g         3769 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaat
//
LOCUS       DAZKOW010000072        14215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000072 DAZKOW010000000
VERSION     DAZKOW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.072"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(33..3686)
                     /gene="rpoC"
                     /locus_tag="ACG912_002047"
     CDS             complement(33..3686)
                     /gene="rpoC"
                     /locus_tag="ACG912_002047"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFQ0513352.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3765..7382)
                     /gene="rpoB"
                     /locus_tag="ACG912_002048"
     CDS             complement(3765..7382)
                     /gene="rpoB"
                     /locus_tag="ACG912_002048"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFQ0513353.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8110..9078
                     /locus_tag="ACG912_002049"
     CDS             8110..9078
                     /locus_tag="ACG912_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0513354.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9096..10001
                     /locus_tag="ACG912_002050"
     CDS             9096..10001
                     /locus_tag="ACG912_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513355.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9998..10804
                     /locus_tag="ACG912_002051"
     CDS             9998..10804
                     /locus_tag="ACG912_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513356.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10961..11929
                     /locus_tag="ACG912_002052"
     CDS             10961..11929
                     /locus_tag="ACG912_002052"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFQ0513357.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12246..12770)
                     /locus_tag="ACG912_002053"
     CDS             complement(12246..12770)
                     /locus_tag="ACG912_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFQ0513358.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13057..>14215
                     /gene="scm"
                     /locus_tag="ACG912_002054"
     CDS             13057..>14215
                     /gene="scm"
                     /locus_tag="ACG912_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFQ0513359.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTT"
BASE COUNT         4029 a         3073 c         2802 g         4311 t
ORIGIN      
        1 aataatttgt cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct
       61 tctgcagcca tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg
      121 acttcttttg gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta
      181 cctataataa cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca
      241 tccgtcaaga cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct
      301 aatgatgctt ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag
      361 actagcgtat tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag
      421 atatctgtat cacctggatc cattacacga actttacgca gcatttgacg aaccattact
      481 tcgatatgct tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga
      541 agcaagtagt tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt
      601 gatccttctg tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt
      661 gctgtataag gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg
      721 gtacgtgttg caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact
      781 gcttgccctt tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg
      841 atatcgtctc cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt
      901 tcaccgattg attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca
      961 gttgcaaggt tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag
     1021 acagaacgga ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg
     1081 gaaatcaatt ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta
     1141 cgggtgtaac gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg
     1201 atggcttgga tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct
     1261 tgggccacgt caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta
     1321 tcggtcatcc ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct
     1381 tcacggaagt tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa
     1441 ccacgcattc cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac
     1501 atcatgaaga ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct
     1561 tttgcagcat tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca
     1621 cgacggaatt gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt
     1681 ttttcattca gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt
     1741 ttgtacccta agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt
     1801 ttgaagactt ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct
     1861 tgctctttga tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt
     1921 tggacggtca agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc
     1981 tcgttgaaga tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta
     2041 aatggttttt cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat
     2101 ccgttgcgcc atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg
     2161 cgtccttctt cttccattgt caagtagtag ttacctagaa ccatatcttg ggaaggagta
     2221 acaactggtt taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt
     2281 gcttctgctt gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa
     2341 tcggcattat aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa
     2401 actggttcga atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact
     2461 gggtgttcct tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt
     2521 ttacgtttcg catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg
     2581 aatggtttga ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag
     2641 aaaggtccaa caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt
     2701 tgacggaaac gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta
     2761 cctggtccag taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct
     2821 tgcagcatac gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt
     2881 ttcaaacggt tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca
     2941 aaacgtccac cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg
     3001 tccataacca tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa
     3061 cggcggattg cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg
     3121 gctgcttctg catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata
     3181 gcagcttgga attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac
     3241 aattgttttt tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa
     3301 atgatttctt ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc
     3361 cctttaaagt accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca
     3421 cggcgaactt tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa
     3481 cggatacgtt tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc
     3541 tcacagaaca acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt
     3601 tttacttcac cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata
     3661 ctttcgaatt tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc
     3721 gcaagacggc tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct
     3781 tctgcggttt caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct
     3841 ttcttttcta attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg
     3901 atcaaatcgt cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc
     3961 aagacacgca tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc
     4021 ggtacacctg gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt
     4081 ccgactacgt catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa
     4141 gcttccagtg cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct
     4201 cccaatggtt gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca
     4261 tcgaccatgt gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat
     4321 ggttcacctg tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca
     4381 cgaactgttt cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg
     4441 atacctaact gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata
     4501 cgtgaaggca cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat
     4561 ggcatatctt cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc
     4621 attttatccc cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt
     4681 acgcctggtg acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata
     4741 ccgccgccgc catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca
     4801 aagatcgcat gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact
     4861 ttccctacaa gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg
     4921 tctaaatctt ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt
     4981 cctaattttg tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg
     5041 tcatctttta ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa
     5101 gtcatgaaac caactaaaac gttttggcct agagccattt cgccttgttc catagatggt
     5161 ccgtctgcca gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg
     5221 ttgtagcttg ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca
     5281 ccgttgtcgc ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt
     5341 ttacataaca aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc
     5401 catggtgctt gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag
     5461 gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact
     5521 tgtttaggtg atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt
     5581 tgagcacgag ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt
     5641 gcttgggcta cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta
     5701 acacgacctg tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt
     5761 actttcgcat aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg
     5821 atcggacaca tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg
     5881 tcacgagtca atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca
     5941 agagggtttg tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata
     6001 cttgccacaa ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg
     6061 atcgacattc tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt
     6121 tgtaaaagtt caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca
     6181 ttgccgatac cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga
     6241 cgaaccgttt ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca
     6301 gggtctcttg gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg
     6361 tcttctgatg gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt
     6421 tccatgactt ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga
     6481 tcaactaatg tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg
     6541 tttactttgt aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca
     6601 ttcagcaagt tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa
     6661 acgtctttca aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg
     6721 actgtattgc gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca
     6781 aatcctaacg cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca
     6841 taagagatgt cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca
     6901 gtagaaccga atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt
     6961 gaacggacta actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt
     7021 tctgtcatta atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg
     7081 cggtttgtca aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga
     7141 gcttctgcta ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga
     7201 ttgccattga agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct
     7261 aagaaccatt ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca
     7321 ctgattcttg cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc
     7381 aagctcttca cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa
     7441 tatttcaaat attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa
     7501 aaccaaaaat agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt
     7561 atattaaagc cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg
     7621 caacttcata tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc
     7681 tttttcgcaa cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt
     7741 aattgacaga cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc
     7801 tatgcgaacc aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta
     7861 ctttagatag gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta
     7921 tgatggctaa agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct
     7981 gagcagtgaa aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga
     8041 cttttcaatc catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag
     8101 gaaaaagaaa tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta
     8161 ggaggttgcg cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc
     8221 gtattgcaac ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga
     8281 ttagcagaag aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa
     8341 aacaatcaag acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg
     8401 ctacttggaa tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg
     8461 atgactgtca cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa
     8521 cctggcggca atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta
     8581 ctgttaaata tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca
     8641 aactctaaga tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc
     8701 aaagtactga cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa
     8761 gatgtagatg gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc
     8821 ggtgaagtcg caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa
     8881 gacggcggtc tagcaacagt aggaatcgac tacgaaagct tgggtaaaca aacagggatc
     8941 atggcagcaa aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat
     9001 gatttggaac tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc
     9061 atcaaagaag ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct
     9121 tatcatctgc atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt
     9181 tccgtatctt ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga
     9241 tcgcggcagt aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt
     9301 tagggggtgc ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg
     9361 cccttcttgc agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg
     9421 gtgcgccgaa catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg
     9481 gcataacaaa agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat
     9541 gtttacttgt tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata
     9601 atctggcaat gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata
     9661 tgatttctaa tggatgcatt gctctttctg gctccttgct tgcgcaaaac aacggctttg
     9721 ctgatttaaa ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag
     9781 aagttttgtt acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg
     9841 tcatttatcg ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga
     9901 aacttgcttc tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat
     9961 tccatttacc tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga
    10021 aaatctccat catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat
    10081 taatctttcc ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa
    10141 atctactttg ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt
    10201 gaatggaaaa aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt
    10261 attccaagat cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct
    10321 tgctatgaaa cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac
    10381 atttttcaaa gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga
    10441 aatcggtttg ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca
    10501 gcgtccagaa ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa
    10561 aacggtgatg gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt
    10621 cactcatgac atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg
    10681 aaggatcgtc gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt
    10741 agcgttgttc aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc
    10801 ataatcagaa agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc
    10861 ttctttcgtt tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc
    10921 cttcttcctg atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt
    10981 acttcaatta ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact
    11041 ggaagtttcc cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg
    11101 ttttgagcac gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga
    11161 aatccagcaa gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc
    11221 tgaatctacg atgaaagatg tcaaactggc agcagctcaa ggagattctg tttcgaagtt
    11281 atttgttgct gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga
    11341 acctggacga ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt
    11401 gcctcaatat acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg
    11461 tcgttctgct tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg
    11521 gattttatct gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg
    11581 gatcgggctt aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc
    11641 catctctgtc tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat
    11701 ctggcaagaa ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga
    11761 aaaatcattc gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg
    11821 tcttgcaaaa gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat
    11881 gagtttgtct tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt
    11941 cacaaaagga cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc
    12001 tccttattct atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc
    12061 tataaagcat ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt
    12121 ttacacgatc aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg
    12181 gtaacgataa aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta
    12241 gtgccttaat ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata
    12301 gcagcatcat caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc
    12361 tcgtcaatta cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa
    12421 tcagcagctc ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt
    12481 gcttgttcca gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg
    12541 ctttcccagt cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa
    12601 tctaattctc gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta
    12661 tcttccactg gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac
    12721 cgatcgtatg cccgatccac actctcgcat agcatatcta aataatccat ttattttcct
    12781 cctttttatt tatcctactt actttttcct aattctagga tacctactta tatgttacta
    12841 cacaccctta ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga
    12901 aaaagatagt gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata
    12961 ttctttaaat aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat
    13021 aataggaaaa tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta
    13081 gtcctagttg ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca
    13141 tacattccta aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta
    13201 ggaattgcta gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca
    13261 gacggaaata tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat
    13321 gaaggtttat tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct
    13381 tcaggcatac ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat
    13441 gtagcacttc aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca
    13501 gatgattttt ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct
    13561 aaattgaatg tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct
    13621 gcagattttc cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg
    13681 tttttattgg taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt
    13741 aatccaaatg atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta
    13801 cagtcaccaa tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg
    13861 aatatctctt ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa
    13921 ggaacaatcc ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt
    13981 ataggaacaa atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt
    14041 atcagcgaca atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca
    14101 agtgatagct cagattcatc aacttcaagc gacagtacag atactacctc taccacaagt
    14161 gatagcacgg atacatcagc ttcaagcgac agtacagata ctacctctac cacaa
//
LOCUS       DAZKOW010000073        14128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000073 DAZKOW010000000
VERSION     DAZKOW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.073"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..179)
                     /locus_tag="ACG912_002055"
     CDS             complement(<1..179)
                     /locus_tag="ACG912_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFQ0513360.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLAS"
     gene            complement(658..1377)
                     /locus_tag="ACG912_002056"
     CDS             complement(658..1377)
                     /locus_tag="ACG912_002056"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFQ0513361.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1426..3072)
                     /locus_tag="ACG912_002057"
     CDS             complement(1426..3072)
                     /locus_tag="ACG912_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0513362.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3251..3841
                     /locus_tag="ACG912_002058"
     CDS             3251..3841
                     /locus_tag="ACG912_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFQ0513363.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3933..5438
                     /locus_tag="ACG912_002059"
     CDS             3933..5438
                     /locus_tag="ACG912_002059"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFQ0513364.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5522..6874)
                     /gene="celB"
                     /locus_tag="ACG912_002060"
     CDS             complement(5522..6874)
                     /gene="celB"
                     /locus_tag="ACG912_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0513365.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6874..7392)
                     /locus_tag="ACG912_002061"
     CDS             complement(6874..7392)
                     /locus_tag="ACG912_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513366.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7408..7734)
                     /locus_tag="ACG912_002062"
     CDS             complement(7408..7734)
                     /locus_tag="ACG912_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFQ0513367.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7747..9726)
                     /locus_tag="ACG912_002063"
     CDS             complement(7747..9726)
                     /locus_tag="ACG912_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0513368.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9747..10061)
                     /locus_tag="ACG912_002064"
     CDS             complement(9747..10061)
                     /locus_tag="ACG912_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFQ0513369.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10229..10522
                     /locus_tag="ACG912_002065"
     CDS             10229..10522
                     /locus_tag="ACG912_002065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFQ0513370.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10600..11712)
                     /locus_tag="ACG912_002066"
     CDS             complement(10600..11712)
                     /locus_tag="ACG912_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0513371.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11865..12713
                     /locus_tag="ACG912_002067"
                     /pseudo
     CDS             11865..12713
                     /locus_tag="ACG912_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13066..>14128)
                     /locus_tag="ACG912_002068"
     CDS             complement(13066..>14128)
                     /locus_tag="ACG912_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFQ0513372.1"
                     /translation="IEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEILQGAVF
                     ELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQAPTGYELDATPIEFEIERSQ
                     TAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLA
                     IDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKID
                     DQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVETQAPTGYELD
                     ATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILS
                     ILGVLLVFISIKFRKKRNI"
BASE COUNT         4378 a         2903 c         2423 g         4424 t
ORIGIN      
        1 aagctagcca agaacccatc tgctgaaata tgaaaatttt tgaatgctac gacattcgcc
       61 attacaggta tgtaaaagaa aaaaatcatc caaagcatac ccggtacagc catcaggaca
      121 agtgctttat aacgccatag attcataaaa aaatgattga acccctttcg ttttttcatc
      181 taaaaacccc tttcgattca ttcgcaacgt aattatagcg ctttcaataa tgagaataaa
      241 taaacaaagt atagggaaaa tgaaacaaag tataggagag gttgtgaaat cagcgttttg
      301 gcctgagtat tagagaaaaa aacgggaaaa tagttcctca tattttgccg ttttttgagc
      361 taataccgag ggtcagctga ttgaacaccg tttatcagag gttgtgaatt cagcgttttg
      421 gcctgagtat tagggagatc gtgtccttac tatctaactc tgagtcacaa ggaacaatgg
      481 ggactgtaac aaaagtcttg tcacagtccc ttattccgaa caaacggtgt tccagaagta
      541 gcttctacgg aaataagccg aaatttcaca aaaaagccat ccgaaagtca gacggacaag
      601 tctgcttttc agatggcgct acaactactc tgcttattct tttagctatc cattcaatta
      661 tttttctctc acttgttcta gctcttcttt ggtcatgggt cgatattgcc ctttattcaa
      721 atctttaggc agccaaaatt ttcccatacg gattcttttt aaatataaga cttcttttcc
      781 tactgctttg accattcgct taacttggtg aaactttcct tcatgcaaaa tcagacggat
      841 aattgaagtt tctgtctctt gatctacttg atcaatttgg agttttgccg gcaatgtctg
      901 ctcatcgcca tcaataatca gtccttctgc aaataaacgg atatcttctt ctgtcattat
      961 accgctaacc tcagctagat attctttctc cacatgtttt ttaggtgata gtaactgatg
     1021 ggcaaacacc ccgtcattcg ttaaaatcaa gaggccctct gtgtctttat ctaatcggcc
     1081 gactggaaaa agatcttccc gataatcctg gtcttccaat aaatcgatta cggtttgatc
     1141 atgattatcc tgagtagcag aaatgactcc tgcaggttta tgcaaaatgt agtaaaaatc
     1201 tttttgatac gtaatgattt ctcctaaata agtaaccgca tcttcgaatt catttacttg
     1261 cgctttatcg cttttcacca cttgtccatt gacttcgatc gttccttttt tgattaatcc
     1321 tttgacttct ttgcggctgc caagtcccat atctgctaaa aacttatcta aacgcatgcg
     1381 tttcccccat ttcaaaagag catcaatcta gctcttctga ttcacttatt ttatttttaa
     1441 tttatgtcgt aaacgcgcta ttcctggtcc tagtagtctt tctgccaagc ggattttcaa
     1501 agctaaataa ccatacacta aagcccctat acctgctact acaagaatca acaatagaga
     1561 ctggaatttg ctatcttcgt ttaaaaaagc accaaaaatc atctttgtga tccacgaagc
     1621 caaaagcata atcaaggtaa gaataaagat cagcaatgtc ctgcgccaga caaaacttgg
     1681 tttaaagcgg gctacttcat ggattctctt taagatcaag gtacaagaca cagcaaatcc
     1741 gatcattgtc gccaataaag ggccgtatac ttcaaataaa cgaatggctg ggtattgaag
     1801 aaggagtttc actagaaacc caataaccaa ataattaatt gccgccttgt tttcaaacat
     1861 tccttgcagc atgttggaaa ctagcatata tagtcctagg aagagtccta caaaacttgc
     1921 ttggatcagt acattacttc ctagagaatc cggtgtataa aataatgtat ttagcggata
     1981 ggcaagtaac ataacaccaa atgtcgccgg aaacataaaa aatgaaaaca actgtaaatt
     2041 tccgcttgtc aattttgcca gacctactcg atctttcaac gtaacagctt ctgtaatcaa
     2101 cggtaaccca gtagacgcga tcgatgttgc caatgcaata actaccatcg tcaacttatc
     2161 tggattggca ctaaaaatcg aaaaaagatc cagtaactgt gcattggaat actcggtgga
     2221 tgcagacatg atcttcataa aagtgaactg atcgactaat ttgaagatcg ttacccctga
     2281 acccacaata ataaacggaa tcgcttcttt aacggtatct aacaacaatt ccttcgttgc
     2341 aatatttatt ttgttttcgc tgtagtgaat aaacgccgat gtatacgctt gatgtttctt
     2401 caaaaaatac aaaaggacaa gaatacttgc tagcataccg ataaatgccg cgaaagttga
     2461 ttgcgtgacc gccgtcacat agtcaccatc catgactttc atgatgatga atgtagccaa
     2521 tagcatataa aaaatacgtg ccacctgttc tacaatctgc gacagcgcat aaggcatcat
     2581 ttcttgattg ccttgcaaat accctcgtat cacactcata caaggaaaaa ccaatacagc
     2641 gatactcaaa gaacgcatcg ttggaatcag ttcttctccg ccaccagaag catgagctag
     2701 ccaaggagag gcgatgtaca tgaacaaagc aaacaacagg cctaacccgc ccatcatctg
     2761 taatgcacgg ataaataatc gtcgtgaagt gccatattca ttcaatgaat tatagcgtgc
     2821 agtttgtttt gcaatcgcag caggtattcc tgctgtagag atcagtagaa acaatgcata
     2881 tatattgtat cccatattga ataatccatt ggcagcctta gcattttcgc ccatccaagc
     2941 ataccaggga atgatataga cagcgcctag caatcttgaa atgatattac tggcggtcat
     3001 ccaagcagag cctcgtgcca ttttttcttg cggtgttaat tgatctaatg ctggttttct
     3061 ttgattatcc atcggtcaga ttccaacttt ctacaaaaca ttactatcat tttaattggt
     3121 aaaacagaaa ctggcaatcg attttatata acttttataa gaacaattgt ttttttgaat
     3181 tcggtttcgt ttttttggta aagtaaagaa atatacagcc ttttatggct aacatttata
     3241 ggagatatca atgacacaag aaataaaaaa actacccgtt atatttttag gtcttttctt
     3301 tatcacagtt ggattgaatt ggttcttgct ccctcacgat atagcttccg ccggagtggg
     3361 cgccatcgga catttagttg aaacggcctt ttctatccaa cgctcagtta ctgtttggag
     3421 catcaatctt atgatgctgc ttctagccgc tgtcctatta ggaaaatcag tattttttaa
     3481 gactgtagcg ggaagtctgc tttttcctgt tgttttagag attgttccaa aagtggaaat
     3541 gatctcctca catttctttt cactgatttc aggaagtctt ttgttcagtc ttggggttta
     3601 cacattgtac acagtcgggg cctcaaatgg cggtataacg ataccgccga ttattttcca
     3661 caaatttttt cggcttccaa tgccgcaagg acttttactt accaatagtc ttatcgtctt
     3721 tttgaattat tatgtttttg gattgttgga gacactattc gttctcctat ctattacagt
     3781 cagttcattg tttatgaaaa tactcatccg cttgtcccct gtgtcaagaa aatttgaata
     3841 aagctggatt ttgtcatagc ggaaacaaac gatatattaa aaagagtgtg ctataataaa
     3901 ccaagaaatt gaataaagcg agggatcaat caatgagctt ctctttagaa gacatccgtc
     3961 atttactatt aaaagaaaat ctattaaaag aatttgtttc aacaagaggc tggcatcttg
     4021 atgtaccaga aaaagctgaa tttcttcagt taagttatga ttcaagaaaa gctgatgctt
     4081 ctactttgtt tttctgtaaa ggaatgaatt tcaaagaaga atatttggat tcagcaatcg
     4141 aacagggtat tcaatattat gtatcagaac aaccatacga aaattcagca gtaggaatca
     4201 tcgtaacgga tatccgtaaa gcaatggcgc ttcttgctat ggctttttac gactatccgc
     4261 aaaataaatt gaaagtgatc ggattcactg gaacaaaagg taaaacaact gctgcgtact
     4321 ttacaaaagc aatcttggat cacacaacaa ataaaaaaac agcattgctt tctactatga
     4381 atacaacgtt agatggcaaa acatatttca aatcccattt gacaacgcct gagtcattag
     4441 atttatatcg tatgatgtca gaagcagttg aaaatggtat gactcatcta gtgatggaag
     4501 tttcttcaca agcttacaaa acacaacgcg tatacggctt gactttagat gtcggaatct
     4561 tcttgaatat ctcgcctgac catatcagtc cgatcgaaca tccgacattt gatgactatt
     4621 tctattgcaa gcgacaattg atcttgaatt caaaaacagt tgttttaaat catgaaagtg
     4681 actattttga tttacttagg gaaacagccg atctattcca gattccaacg atcacttatg
     4741 gtcgttcgga agaaagcaat tatcaagtga tccgttcaga taaaggtact cacggtttta
     4801 ctttatcttc tcatgaagat cgcttagcta tctgcgatac tgcttatgat attttgctgg
     4861 ctggaggatt caaccaagaa aatgcagcca gtgcgatcat tgccgcagca ctaacaggtg
     4921 cctccagtca agatgcacaa gacggattaa aagaagctcg tgtaccaggc cgaatggatc
     4981 aattgatcca atccaatgga gcgcatgtct atgtggacta tgcacataat tatctaagct
     5041 tgaaaacatt gctgaaattt gccaaaaatg aacatccaga tggccgtgta attgttgtat
     5101 taggcagccc aggaaacaaa gcaatctccc gtcgacatga ttttggaaaa gtgctatcag
     5161 aaacagctga catcgccttt ttgacggcag atgacccagc atttgaagat ccgaaaaaaa
     5221 tcgcggaaga aatcaatgaa gcaattacta atccagattt gatcgttcat tatgaaatgg
     5281 atcgtcctga agcgattcga cgcgctttag cagaaagcgc tccgcaagat tcagtcgtta
     5341 ttgcgggaaa aggcgtggat ccctatcaaa aaatcaatgg tgtagatgaa ccttatgaag
     5401 gggattatgc aatcgtgaag cggttgattg aagaataatg gtatttatat actagtggtt
     5461 tcccatttct aagaaaaaaa gggactgtga ttttttgtca cagttccttt tttcttagaa
     5521 atcattttaa cttttcacta gctaattctg ctgccttttc tctttcttca tttaataatt
     5581 tattatcata gaatttgata aatgggaacc agataatgaa tgcgatgatt gcacaaatga
     5641 ctgctaacac agcagctttc caatcaccac cactaccaac aaatgctccg atcccgactg
     5701 gtgaaggcca cggcatctgt gcaagcatcg gtttgacgat ttccagccga atggcaaaat
     5761 aagcgagtgt agcagaagcc atcggagcta agaaaaacgg aaccgctaaa tagggattgt
     5821 agacgattgg catcccgaaa atgattggtt cattgatatt gaataatcct ggtacaagtg
     5881 aggcttttcc taacactttc agctgatcag atttcgcgag gaatgcaata aagatgatca
     5941 atcctaaagt cgcacctgat ccaccaacgg ttacataaga attgttgaat tctcctgcca
     6001 acggataatt tgcgccttgt gcgttagctg ccatattagc taatacgatc ggggtcaaga
     6061 aagaagtgat gatattcgct ccatggatcc ctacgatcca caatgcatgg atcaagaaat
     6121 aaatcaccat gacaccaagc catgtattcg tcaactgcgt aacaaaccca aatgggatag
     6181 caatcatttt gaagatatcc gttcctagtg caactaataa tccattgatg atcatcacca
     6241 caaacgcaat gacaaatgtt gggatcagag cagtgaaaga acgagaaact cctggcggaa
     6301 caacatctgg catttttatg acccaatttc tcttgacaca catacaatac aattcgaccg
     6361 caataatcga cataataata gctgtaaaga ttcctgaagt tcctaaacgt tcgacaaaag
     6421 agcccatcct aataccattt actacagttt ctgtctcatt gatagaagca atcaagctga
     6481 ttttaccatc agtcatgatc aattcaggga tacacataaa gaatgcaaaa acagatagta
     6541 atgcccccgt gagcggatta acatttaatt tttgttcatc tcgttcaatt gacgttaatt
     6601 cataagcaaa cacaatggca aagtaaatag ataaaatccc catcgtcatt gtattgacga
     6661 tcatgtacaa gtcactaact ttgaaaaaag ttgcattaaa aaatccttct aaaaatggga
     6721 acggtaatgg caaaatattt aaaaccaaaa acatcgagcc tacaatcgtg aaaggaatag
     6781 aagccatccc cgctgccatg acagcccgaa cgattttaag ttgcgcaatt ttccccatag
     6841 gtcccatcag gtatttctgc aaaaaatcaa acattagtta ttctcctttc ctaaatataa
     6901 cgctttttca tattgtttaa tacggatata ctgttggtct tggttgagtg aaattttctt
     6961 taaacgatac tgtataaatg cactggcaaa aataccgctt acaacaagaa tgaaaacaaa
     7021 taactgtcct ccattgttat ttgccataaa aggatacaag aatgtaaatc ctgaggtaaa
     7081 caacactgga atcactaata aattaaagac actctggaac aaaaaatacc actttgtcca
     7141 tggaacgttc gtttggtgtt ttcgataaag ctttacttgt tctcctactg caggcaaaat
     7201 agctaaaatc agaatcatcg gtaaactact tcctaaactt ttagctgata gaagcagaat
     7261 catccattgc atgttcatga aaaagaaaaa ggcagtcagg taacgtacga ttagataacg
     7321 attgaaatac atgtttttca aactaagttg ctttttttct aattcgattt tcctttttac
     7381 tgtatcctgc acaatgatcc ttcctttcta tttatttttt tgatacaaat aattcatttc
     7441 ctgtgcaact tccaataatg tcatcgtcgt catcagatga tcttgtgcat ggaccatgat
     7501 aatttccatt tctatttttg tcccgctggc atattctttc agaagttgag tttgtgactg
     7561 atgcgcatga agaatctctt catttgcttg tcgcaacttc tcttctactt cttcaaacat
     7621 ctctttacgc atcgagcgaa atgcttcatg tatcagtgtc cgtgcatttc cgctatggaa
     7681 aatgatatca aaagaagtca cttgtagtgt ttcactgttt attttttcag ccatctgttt
     7741 tcctccttat tcgatcagtt tgataaattt actgctgaaa ttctcaaaaa taggttcagc
     7801 taagatttcc tgctgtactt ttagctggtc aactaaccta acgattgctt tagtcacgac
     7861 cgtaattcct tcattttcga cataagaagg cgaaactaaa aaaacaaaat gaatttcttg
     7921 atattgatcc cactgcatcc cagaaggaat caatgcaacg gctacctttg tttcgattcc
     7981 aactggaaca gcgggatgtg ggactgccac tgtatcactg aatatgattt gtcccatatt
     8041 ttcacgttgc tggatctgct ttcgcatttc tcttatatat ccttcttctt catttatgct
     8101 cagcttttgc agcaaatcat gaagaatttc ttcctttttc ggttcgtacg tataagtctt
     8161 gaaaaatttt tcagacaact gttcggtgta aatctgcttt ttttgctgta gagaaaatgc
     8221 tggtatcggt ctatctgagg aatgataggg tatagattct ccaactacct ttcgtatttt
     8281 acgaacatca tcttcattca aaaaaatact gacatgtaaa actggaattt tgaaaaacat
     8341 tgtcgaaaga tcaatagaag aaataatcag gtcgattcct tttaaagttg tttcattgat
     8401 ctcataatag ccttttacac ttaccactgt aatattttta ccgaattcac tgactacgcg
     8461 attctttaat agttgggcac tgccgtaacc cgttgcacag ataatcaatg catgtagttt
     8521 atgcgcagct ctttcttttt ccaatgccgc catgagatgc aaagccaaat aggcccattc
     8581 atcttcattg atggtatagt ttttcaaagc cggcatgttg ctgaaatgcc tttttactgc
     8641 gtcaaagact ttgggatttt ctttctttat ctcctcagtc aatgggttct caatgcgaat
     8701 acctcggctc aatcgaatca tcatcggtga caaatgatcg agtagtccat tgaaaagctg
     8761 gagatcctct actaatgaaa aatgaaattc ctgagacaat cttttcagaa ctttcgctaa
     8821 ttcctttgat agttcctgat cattttgatt ttctttgtga ttcgacttag ccatgagatg
     8881 taatgtcaaa taagcaattt cttcttcggg aaacgataca cctgcgatta cctcaatacg
     8941 ttgaacgatc cgctgcgcca cttgatactc tactcgctta gaaacttctt gttcgatgcc
     9001 taattccttg atttcaaacc cttctcggag ccgtttgatc cccaaagcca agtgtaaagt
     9061 cagattctgt atgataaaat ccgaaacttt taacttagct tctcttgttt catcgagaat
     9121 gataatgact agctcttcaa aactgatgtc tgggaaaaaa cggctttgcc ctaaatattc
     9181 tttcaatgaa tttccataat ggtttccaaa aaaagtatcc atgataaaat gccgtcgatt
     9241 tctttcttcc ccttcgatcc atgtcccttg accatgctta gaaacgattt ttagtgaata
     9301 tggaatcaat cgctccttga tttcttgcat gtcctttgtc aaactggaac gactgatata
     9361 aagctcttct gccaaatcat ccataaaaag aatggcatct tccaaaagca attggttcaa
     9421 tatatacttt tgtcgatctt cggactcata aaatccttgc ggttctctag catatccagg
     9481 tactatttct tgccgactca aaaacaagtc aaatgtcagt ctttgtaaaa tatgcagttg
     9541 gtagccataa cctggtttag caatgatctt acctccgttt ttttctacta actctttgat
     9601 ccgctgcaag taattacgga ttgtccgatc agataataac aattcagaag ctaaacgttg
     9661 actggtgaca tactccttct tgtgatcaat caaatatctc aacagcaatt tttccttctg
     9721 tttcattatc atccactcct attccatcat atgttctctt cgatcacctc tgctaacttc
     9781 tggatgcctg tagggattgg gacatacgct tggaaaggga tactaacaac aggctttcca
     9841 acagattctc ctaatttttt gaatttatcc aaaaacatcg tcgtttgcgg gctgatcaga
     9901 aataaatcaa aatcactatt cttaatcata ttatcgcctt ctgtagctga aactgcatct
     9961 acgagtacct cttctctttt ttgcttaaag aaatccgttg tctttgaggc catcatcgag
    10021 gatgacattc ctgccgcaca aataatcaat gcttttttca taaatcaatc actcctttta
    10081 tttttataaa ccaagcatat cttgtaagcg ttttctattc aaaatatata tttccgtagc
    10141 attgcggaaa aaatgtgtat catatcttga ataataattt ctggcaccgt gatatactag
    10201 tgatagaatt ttttcaggag gggatttagt gagtatcaca gtgaatattt tgtatacaag
    10261 tacaaatgga caagccagaa aatttgcaga agaaatggtc gaaagaaaca ttgtcaaagc
    10321 gattcgtgca gaagaaggaa acgagaaata tgactattac tttccactag aagatccaga
    10381 atctctttta ctgatcgacc gttggaaaga tgaagaagca atcgaaaagc atcacaaatc
    10441 cgaaatgatg gctcaaataa acgaactccg taaaaagtat aaattaaaaa tgaaagtcga
    10501 acgtttcact gatttaccgt aaaaagatac acatggaatg atagcaattt atcatccatg
    10561 tgtatcttca ctttttcctt attttttatt cgatctcatc taatgattca aaccgtgttt
    10621 ttctcttatt tttcggataa gtatttcagt caccccaata tacatgattc ctaaacatac
    10681 cccaaggata ttctcaaaaa tgcggatcgt tactgctcct ggaaccccga ataaactggc
    10741 agtgattgcc aaagcaccaa aagaattaaa aactgtttta tatctatagg tagaacaaac
    10801 acctaaagct agaccaccta atataccgac ccagttaaaa ggaataaatt ggctgatacc
    10861 aataaataaa acacatccaa tcaatgagcc aatgattcgg tctactgctc gttctttgaa
    10921 accaatcgac atcaatccat agctagatac aatagaagaa aatgcaaacc ctgcccacat
    10981 caatcgctga aaagggacat actctcctgc aacgatcaaa agactaatgc caaatgcata
    11041 gatgagcatc caaatttttt cctgcgaata catgcctttc aaaaaaatct ctttaaacaa
    11101 gcttttgtct ttgttctttt ctcgatgttt tctgtaaagg agaactgaaa accaacaaaa
    11161 aaacaaaaat aataagctac ttgtttgtgt aaagaaattc ttgttcaacg aatctttagg
    11221 taacgaataa acaataaaga gatatgaaaa gccgtacaaa gaagcattcc ccattttggg
    11281 atcactagcg gttgccatta atatgctgct tagtaaaata aaatgaatca acatgcccat
    11341 aactgaccat ttgatcagct ggacatacgg tgctactaat agtgagaaca tgacgattgc
    11401 taaacctatg atcgaatggg aaatcttata accaaagtca acaaaacgaa tactcatcaa
    11461 catacagaat aaaaccacag cgtatggttt atgcttcgat tcaaataatg ttgtaattat
    11521 aatgataaaa caaatggcaa acaatacgat gaataatgat cgtaaaaaca atgaacaaat
    11581 atacatgcac ttttcttttt tagaagctgc ttctcgtatt ttttgtttta aaataaacgg
    11641 atctagttgt agagactgat aaaaatccac ctgttgtcgt cctttctttt tacttttatt
    11701 ttttacagtc atctatgaca ggttgtaaat actatggaac atcatatggc gttgtcattt
    11761 tcttgtcaac tatttttcct attcatgttt ttataaaaaa acatatcgtt ttcttgtatg
    11821 attaaatcac cacaaaataa acaactgaga gaacgatatg ctttatgaat ggttctatca
    11881 aaaaaattct gagatcaata gacaaaaatt tgatgattac agaggtctct tacgaaacct
    11941 tacagaagaa aaaaacattg atcgttgctg cagttctctt ccctgctcca tgtgtctgta
    12001 gaaattgtgg ttctgctgtg gtagatggaa ataggaaatt aatggtggtg aaaaacaaga
    12061 agaaggaaac cattgtccac tttgaataat ataatcatat gccaatggtt atacgcctaa
    12121 aaaagcagcg ctatacctgt aaaaactgtc gaagccattg gaacgctcaa agttatttta
    12181 tccgtccaag gcactcgatt tcaaatcatg tcagacataa aatcacttct ttactgactg
    12241 aaaaagtgtc gttatttttt atttcgaaaa gttgttaggt atctttgaca accgttattc
    12301 gcacattgaa agagttgaga agctatttac caaagtcttc caaaagaatt ctgtcttgag
    12361 tgttgagggt tgtgaatttc gttctcacgt ttccgttgag gataaactga gttttatttc
    12421 cgcggatggc gagacagaaa aattaatcga gaaaatttac ctacacaaaa aattcctcga
    12481 ttaacaaact atttccttag ctgcactaat cctaaagaaa tagaattctt agttacagac
    12541 atgaacgcca cctactttca gctcaccaaa catgttctac caaatgcgaa attggtgatt
    12601 gatcgattac ttggtttttc cacgcctttc aaggaggcgt aacctttatt cacgagttgg
    12661 ttgaagcttt ccgagacaaa agacccgact tgtttttctc cttattgaca aagagccatt
    12721 aaaactagag aaaaaattac aactgatcgc tgattatttg tagaaagaaa atcaaagtat
    12781 tacccaataa acttgagtca ttaggcaaaa aacactgcga aactcaagaa tgattttcgc
    12841 agtgtttttg tatatttttt attaactagt attactacaa aggtttattt ttcacttctc
    12901 aacgcagcac aaaattgtct ttacttattt actggttggg ttcaaccttc tctactccga
    12961 tactaatatt tgtttgtata ccccttctaa aatcggtagt ttccattcaa ttgatgggta
    13021 ctagttgatt ttaaaaacta tgcttattca attttttatt ttagctcata tatttcgttt
    13081 ctttctaaat tttatactga tgaaaacaag tagcacaccc aatattgaca atataaactg
    13141 gctaatgatg gtttctccag tttttggaag atgcttattt ccactgcttg ttggattgtt
    13201 tcctcttata tctgagctat cagcaacttt tctattctct tttgttaaaa agatcgccac
    13261 ttcttgttct ttttcgatct tgaaagttac tggtgtcgca tccaattcat accccgttgg
    13321 cgcttgagtt tctaccagct gatatgctcc tggtgctaag ccatctatcg ccagttttcc
    13381 atctgctcct gtggttagac ctgactgcag aacggttcca ttggcatctt gtaactcaaa
    13441 aacagctcct tgcaggactt ctccgctttg gtcatcgatc tttgtcaaca cgacaccgcc
    13501 tggtgtaagt cgattttctt tcgtcaattc taccaccgct gtctgacttc tttcgatctc
    13561 aaactcgatt ggtgtcgcat ccaattcata ccccgttggc gcttgggttt ctaccagctg
    13621 atacactcct ggtgctaagc cgtcaatcgc cagttttcca tctgctcctg tggttagacc
    13681 tgactgcaga acggttccat tggcatcttg taactcaaaa acagctcctt gcaggacttc
    13741 cccgctttgg tcatcgatct ttgtcaacac gacaccgcct ggtgtaagtc ggtttgcttt
    13801 cgtcaattct accaccgctg tctgacttct ttcgatctca aactcgatcg gtgtcgcatc
    13861 taattcatac cccgttggcg cttgagtttc taccagctga tatgctcctg gtgctaaacc
    13921 gtcaattgcc agttttccat cttctcctgt agttagaccg gtttgtagcg tttctccttc
    13981 tcggttctgt aactcaaaaa cagctccttg caggatttcc ccgctttggt catcgatctt
    14041 tgtcaacacg acaccgcctg gtgtaagtcg gttttctttc gtcaattcta ccaccgctgt
    14101 ctgacttctt tcgatctcaa actcgatt
//
LOCUS       DAZKOW010000074        14111 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000074 DAZKOW010000000
VERSION     DAZKOW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.074"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1364)
                     /locus_tag="ACG912_002069"
     CDS             complement(45..1364)
                     /locus_tag="ACG912_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0513373.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="ACG912_002070"
     CDS             complement(1361..2014)
                     /locus_tag="ACG912_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0513374.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="ACG912_002071"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="ACG912_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(2820..3539)
                     /locus_tag="ACG912_002072"
                     /pseudo
     CDS             complement(2820..3539)
                     /locus_tag="ACG912_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(3490..3699)
                     /locus_tag="ACG912_002073"
     CDS             complement(3490..3699)
                     /locus_tag="ACG912_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513375.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(3681..3749)
                     /locus_tag="ACG912_002074"
                     /pseudo
     CDS             complement(3681..3749)
                     /locus_tag="ACG912_002074"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
     gene            3976..5249
                     /locus_tag="ACG912_002075"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="ACG912_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="ACG912_002076"
     CDS             complement(5356..6387)
                     /locus_tag="ACG912_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513376.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="ACG912_002077"
     CDS             complement(6394..7224)
                     /locus_tag="ACG912_002077"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513377.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="ACG912_002078"
     CDS             complement(7221..8036)
                     /locus_tag="ACG912_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513378.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="ACG912_002079"
     CDS             complement(8033..8857)
                     /locus_tag="ACG912_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFQ0513379.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="ACG912_002080"
     CDS             complement(8847..9947)
                     /locus_tag="ACG912_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFQ0513380.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="ACG912_002081"
     CDS             10125..10910
                     /locus_tag="ACG912_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFQ0513381.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="ACG912_002082"
     CDS             10943..11326
                     /locus_tag="ACG912_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFQ0513382.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="ACG912_002083"
     CDS             11402..11533
                     /locus_tag="ACG912_002083"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0513383.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="ACG912_002084"
     CDS             11502..11738
                     /locus_tag="ACG912_002084"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0513384.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            11750..11836
                     /locus_tag="ACG912_002085"
                     /pseudo
     CDS             11750..11836
                     /locus_tag="ACG912_002085"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(11816..12787)
                     /locus_tag="ACG912_002086"
     CDS             complement(11816..12787)
                     /locus_tag="ACG912_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFQ0513385.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13421..>14111
                     /locus_tag="ACG912_002087"
     CDS             13421..>14111
                     /locus_tag="ACG912_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HFQ0513386.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPPFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQ"
BASE COUNT         4308 a         3049 c         2463 g         4291 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
    13381 gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag tgatagctat
    13441 gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga ttgatgatct
    13501 agttcctcaa gatcatctcg ttcgaaagat tgatgccgcg cttgattttg aatttattta
    13561 tccaatcgtc gaagccactt actccgattt aggtcgccca agtatcgatc cagtgattct
    13621 gatcaaattg gtgtttattc agtatttatt cggtatccgt tccatgcgac aaaccattaa
    13681 ggaagtcgat accaatgtcg cttaccgctg gtttttaggc tactccttcg aggagaaaat
    13741 cccccctttc tcaacttttg gaaagaatta tgttcgccga tttagagaaa cgacggtgtt
    13801 tgaggatatt tttgcctata ttttagaaca agcagttaaa gcaggttttg taacagagga
    13861 taatttgtat ctggattcta cccacatcaa agctaacgcc aataaacata agtttacgaa
    13921 agaaatgacc catgatgaag cgaaagccta tcaggatgaa ttagaagatg agatcaatcg
    13981 tcaacgaata gaggctggaa aacgcccttt tactttggac ttagaaaagg aagtgaagct
    14041 gaaagaaaga aaaataagta aagctgatcc ggaaagcggc tactacgtca aaggcgaacg
    14101 agaaaaacaa t
//
LOCUS       DAZKOW010000075        13977 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000075 DAZKOW010000000
VERSION     DAZKOW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.075"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            140..460
                     /locus_tag="ACG912_002088"
     CDS             140..460
                     /locus_tag="ACG912_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513387.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="ACG912_002089"
     CDS             912..1112
                     /locus_tag="ACG912_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFQ0513388.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..4048
                     /locus_tag="ACG912_002090"
     CDS             1259..4048
                     /locus_tag="ACG912_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFQ0513389.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            4095..4622
                     /locus_tag="ACG912_002091"
     CDS             4095..4622
                     /locus_tag="ACG912_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFQ0513390.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            4643..5833
                     /locus_tag="ACG912_002092"
     CDS             4643..5833
                     /locus_tag="ACG912_002092"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0513391.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5873..7171
                     /gene="asnS"
                     /locus_tag="ACG912_002093"
     CDS             5873..7171
                     /gene="asnS"
                     /locus_tag="ACG912_002093"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFQ0513392.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7966..8640
                     /locus_tag="ACG912_002094"
     CDS             7966..8640
                     /locus_tag="ACG912_002094"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFQ0513393.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            8637..8978
                     /locus_tag="ACG912_002095"
     CDS             8637..8978
                     /locus_tag="ACG912_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513394.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            9008..9367
                     /locus_tag="ACG912_002096"
     CDS             9008..9367
                     /locus_tag="ACG912_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFQ0513395.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9627..9749
                     /locus_tag="ACG912_002097"
     CDS             9627..9749
                     /locus_tag="ACG912_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513396.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEEKKKE"
     gene            9891..11975
                     /locus_tag="ACG912_002098"
     CDS             9891..11975
                     /locus_tag="ACG912_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0513397.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            12286..13752
                     /locus_tag="ACG912_002099"
     CDS             12286..13752
                     /locus_tag="ACG912_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFQ0513398.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4635 a         2430 c         2974 g         3938 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggaggaaatc tatcaggaca tccatgaatt
     4021 cttaaagaaa aatgaagaag gaagttagag gagctgtctt ttttcataat taaaatctgc
     4081 tataatgaaa aggaatgaag ataaaggagc gacgaccctt gaagaaaaaa cagacaaagg
     4141 aaaaaacgat taatcgcgtg ttacttggct tgatcgtatt tttattagcg gtcatcgtga
     4201 tcagctcgat tttttacttg cgttccagtc gtccggcggt gcaggcgaag aaagaagcag
     4261 tagaaatcgc aaagaaatat gcaaatcttg aggaagccga tcatttttat tggtttactc
     4321 gaaagaagac atacttttca ctgacaggta caaatgataa aggaaaagaa atcgccgtta
     4381 tcattcctaa atctggtgaa aaagtaaaag tattggatca aacaaaggga ctgactgaaa
     4441 acgaagcaaa acagacaatt gctgcggccc atcctgaaat ccaaatcgaa aaagctgctt
     4501 tagggatgta tgatgatcag cctgtgtggg aagtagtagg aaaagaaaca aatgggaaac
     4561 tgaattatta cctgattggc tttgaatctg gagatgaagt caagacaatc aagggaattt
     4621 aaaaggtaaa ggaatgaatg ggatggagtt atcaaaaaga acaaaacgat tagaaccgtc
     4681 tgtcactttg gcagctgcgg caaaggccaa ggcattaaaa gcaaaaggca gagatgtgct
     4741 gagtttgact gtgggagaac cggattttgc gacaccagaa aacatccaag aagcagcaat
     4801 cgaagcaatc cgcaatggga aagctagcta ttatacacca acagcaggaa tcccagagct
     4861 tcgacaggca atcgttgatt atctgaagaa aaactatgga cttgtctatg aaccttcaca
     4921 aacagtcgta acagacggag cgaaatttgc tttgtatacc cttttccaaa cgatccttga
     4981 cccgcaagat gaagtcatca ttcctgtgcc ttactgggtc agttacggtg agcaagtaaa
     5041 actggcagaa ggacttcctg tctttgtgaa aggggaagaa tccaatagct ggaaagttac
     5101 tgttgaacag ctagaacaag cacgttcaga aaaaacaaaa gccgtaatca tcaattctcc
     5161 atcgaatccg actggaatga tttatagcaa gaatgaactt caggcaatcg gtgaatgggc
     5221 agtaaaaaat gatattttga tagttgctga tgatatttat ggtcgtttag tttataatgg
     5281 caatgagttt actccaattg caacgatttc agaagcaatc aaaaatcaga ccatcatcat
     5341 caatggcgtg tctaaaacat atgcgatgac aggctggcgc attggttatg cggtggggaa
     5401 ttctgagatc atcaatggaa tgattgctgt tgcttcccag tcaaccagta atccgacagc
     5461 agtcagtcaa tatgcagctg ttgaagcatt gaaaggtgag caagatacag tagaagaaat
     5521 gcggaacgcc tttgaagaac gcctgaatac gttatatccg cttgtggcag aactgccagg
     5581 tgtttctctt gaaaagccgc aaggagcttt ctatcttttt cctaatgtaa aagaaacgct
     5641 acgcatgtgt aaatatgaaa atgtcacaca atgggtagaa gatcttttag aagaaacagg
     5701 ggtagcgctt gtgactggcg aaggctttgg ggcaccagaa aatgtccgca tgagttatgc
     5761 tactgatcgc gctacgttag aagaagcggt acgcagaatc aagcaattta ttgagagtaa
     5821 aagtcaaaat taatcctgaa gaaattcaag gaatataaaa ggagagtcat acgtggaaca
     5881 aattcaaatc atcgattcga aaaaccatgt gggagaaaca gtaacaatcg gcgcttgggt
     5941 cgcaaataag cgatcaagcg gaaaaatcac atttttacaa ttaagagacg gaacagcctt
     6001 tttccaaggt gtagttgtaa aaagcgaagt gtcggaagaa gttttccaaa cagctaaaaa
     6061 tctacaccaa gaaacatccg tgttagtaac tggagaaatc cgtgaagaca gtcgttcaaa
     6121 atttggttat gaaatcggaa ttacaaatgt ggaagtggta agcgagagcc acgattatcc
     6181 gatcacacca aaagaacacg gaacagactt cttgatggat catcgtcatt tatggctgcg
     6241 ttcttcaaga caacatgcga ttatgcagat cagaaatgaa atcattcgtg caacgtatga
     6301 attctttaat caacatcatt ttgtcaaaat tgatccgccg atcttgactg gttctgcacc
     6361 agaaggcact acggatctat tcgaaacaaa ttactttgat caaaaagctt atttatctca
     6421 aagtggccaa ttatacatgg aagctgctgc tatggctttc ggaaaagtct tttcattcgg
     6481 tccgactttc cgtgctgaaa aatcaaaaac acgacgtcat ttgatcgaat tctggatgat
     6541 cgaaccagaa atggctttca ctcatcaaga agaaagttta gaagtacagg aacaatatgt
     6601 agcattctta gtacaaaatg ttttagatca ttgcgaatac gcattagatg tactaggacg
     6661 agacaaagaa gtcttaaaaa cttatacaaa attgccattc cctcgtatta cttacgatga
     6721 tgcaatcgaa ttactgaaga aaaatggatt tgatgacatc gaatggggcg aagactttgg
     6781 ttcacctcat gaaacgttca ttgcgaattc attcgaacag cctgtcttta tcctaaacta
     6841 tccaaaagcc atcaagccat tctacatgaa accacatcca acaagagaag acgtggtaat
     6901 ttgtgcagat atgatcgcac cagaaggtta cggtgaaatt attggcggaa gcgaacgggc
     6961 aacggactat gatttcttgc tagaacaaat ccgtaatcac ggtttggatg aaaaagaata
     7021 ttcatggtat cttgatctac gtaaatacgg ttctgttccg cactctggtt ttggtctagg
     7081 tcttgaacga acagtcactt ggattgctgg tatcgaccat gtacgtgaag ctattccatt
     7141 cccacgtttg ttgaaccgaa tttatccata agaggttgtg cgcctgccgt tcagctccga
     7201 gtcacaagga gtaatttcaa aaaacagttt ttcatgtttg atgactattg tgacttgtgg
     7261 ccggagagca acgtttattt aaaaaaaaga actgcgaact ctcgcagttc ttttttttaa
     7321 atacgggata atggaagttt ccagcagaca agagaagaaa gcaaaagaaa ttgctatttg
     7381 attcacaagg ttctataacg aaatcgcaat caatcaaaca tttacaaaga aggtaattca
     7441 aatggaaatc tgatttgatg atgcttctgc tgttgaaata aagcagcatc tagcacctga
     7501 aaacaaacta atagtaacat tcgaagatgg agtaggtccg tattttcagc atgcaataat
     7561 ccatatgtaa gttcaattca cccttgaaat catcggacca gataaagtta tgaccaatct
     7621 atcccaagca atctagaaga gatattagta aagggacatt tactagaagt cttagatgag
     7681 cctataaata tatgtttcga tggcgtcatc agtggtctga ttgacggaag cttgggtgtg
     7741 atcgatctct ctggaccaaa caacgaagga atcaagggga atccggcacg ttaagaaaaa
     7801 aatagcataa aaaactagac acttgatgag cgttcattca attgtttgaa ggaactaggc
     7861 tggtgtcatt ttttatacat atattttctc tttcgtcttg atagggctta tgggataata
     7921 taaggaagaa aatcatcgtt ttattcaaaa aggagttttt gacaaatgaa aaaacgtata
     7981 ttatttatcg gtgatagcat tacagacgca ggaagatacc gagcagaagc aactgatcta
     8041 ggtaaaggtt atccactttt gattgccgga aaactaatct cccaatatcc aacgatggaa
     8101 tttgaatgca tgaaccgtgg gataggagga gacaaaattg cagatctgca agtacgctgg
     8161 caagaagatt gtttagcttt acaacctgac tatgtatcga ttttgatcgg aatcaatgat
     8221 acctggcata atactagcga tcaggctata ttcggaactg agaaatctgc tcagcaattt
     8281 gaatccgttt atcgaaaatt actaaatgaa cttcaaacag cggaaatctg caacatcatc
     8341 ttgatggagc cgtttgtact tcctataacc gacgaccgtc atggctggcg taaagactta
     8401 gaccctaaaa tccaaattgt tcgtaaactt gctaaggaat atggcgcatt gctggttcct
     8461 ttagatggta tattcaatgc tttagggatt gcttatggtt tttcgaaata tacaagagaa
     8521 gatggggtcc acccaacgat tggcggacat gagatcattg cacaaagctg gatagatgca
     8581 attgtcgcag tcgatttttt tagtggagat aaaaatgagc gaaagaaaag tgaaaaatga
     8641 tggaaataag gtgggcagta aaagaagatt tacctcaaat caatcagctg attactgatc
     8701 atccagaatc attcgtaaaa gcaaaaacgg cagctgacct aaatgataag aataaactat
     8761 tgctcgttgc tgtaacaaaa gaaaaacgga ttatcggatt tgcgcaagcg gacgtgcaac
     8821 agtcagaaga acgattggat cagcgaccat caggaaaact aatccaacac acattagtaa
     8881 caaaagaaaa tacggaagaa atccaaaaaa aatttgcaga atacttgatg ctggaagcta
     8941 aaaaaagaag aataaaatca atttttgccc agttttaatc aaaaacagta atagaaggag
     9001 aaatacaatg agttgtttag gaaatgtcat ctggtttata ttcggcggac taatcggtgg
     9061 tcttagctgg ttagcagcgg ggatactttg gtgtatcacg atcataggta tcccaatcgg
     9121 tatgcaatgt tttaagatcg cctccctttc ttttgcgcct tttggcaaag aagtaattta
     9181 tcaccagtct ggcatgtcat tgatcgttaa tatcatctgg ctgatcgttt caggcctacc
     9241 aattgctctc gcacatgtcg tcagcggcat ccttctatgt atcacgatca tagggatacc
     9301 ttttgccaag caatctttca aacttgctcg attggcgttg atgccattcg gtgcagaaat
     9361 tgtataaaag agaataataa aggttagata aagcatagag ccaggtggga tacatgtatt
     9421 tcacctgact ctatgcgttt tcctgtataa gttcttctat gtgactttta cgtcttttat
     9481 ttatatgggg ttactgttta tggaaagttg aaataatttt cattaaagaa ctctttctat
     9541 atttttgaaa gcgtagtgga gcttcgtata tctgggaagg acattttgca gggattgaaa
     9601 aaagatattg tcctatcgaa aaattaatga atttagacaa attgccagca acaggcttca
     9661 aacttagttg ctatcctgtc aagatcaaga aggcaagtgc gggttggata agagcagtgg
     9721 caatgatcga agagaaaaag aaagaataag ccaaaacatc tgtatggaag aaattttccg
     9781 atgcagatgt ttttcatttt ttctttcgga taccttgaca tatgaataac cattcattta
     9841 taattagtta tgaagagata ttcatatgtt gaggcgagga ggtcattaaa atgaaaagtt
     9901 acagattaga aggtttagat tgtgcgaact gtgcaatgaa aatcgaaaaa ggagttcaga
     9961 aaatcaatgg agtcaaggaa gcaacggtaa acttcacttc tggaaaacta acgattgacg
    10021 cagaggaaga tcatttggct acgattgaac aagaaacaaa aaaagtagtg aaggaattag
    10081 aaccagatgt caaagtaaca gaaatagata aggaaaaagt atctgaacat ggaaatgaaa
    10141 aaaagagaaa tacgctgttc cggattttat tttcccttgc aggtatcgct ttattgctat
    10201 tatttgattt taatgaacca attcgcttga tagggtattt attgatttat ctgctgatag
    10261 gatatgatgt agtgaagaag gcagtgatga atatcgtcaa aggaaaaatc tttgatgaga
    10321 attttcttat gtctgttgct acgattggcg ctatgttgat cggtgaatat ccagaagccg
    10381 tagcagtgat gctattttat caaatcggcg agtatttcca aggattggca gtcagtcatt
    10441 caagaaaatc gattcgcgaa ttgatggcga tccgtccaga aacagctcac gtgcagacgg
    10501 cggaaggatt gatgaccgtg aaccctgaag acgtgctgat cggtcagttt gtacttgtga
    10561 agcctggtga acgtgtaccg ttagatggaa cgatcatcga aggagaatca ttagtcgata
    10621 cgtcagcact tacaggagaa tcagtcccta aatctgtata tgttggtgaa acagtattaa
    10681 gcggatttat caataaaaat aaaccatttt tagttcaagt agaaaaatct tatgaaaatt
    10741 ctactatcag caaattatta gaattagtag aaaatgccag cagtaaaaaa gcaccagctg
    10801 aaaatttcat tactaaattc gcgcgctatt acacaccagt cgttgtagga ttggccgttt
    10861 tattagctgt tttacctcca cttgttgttt ctagagcggc gttttctgaa tggatctatc
    10921 gagcattgac atttttagtg atctcatgtc catgtgcatt agtgatttcc gtgccactta
    10981 gtttctttgg cggtatcgga ggagcaagta aaatcggtgt attagtaaaa ggttcaaatt
    11041 acttggagtt gcttgcacaa actgagacag tggtattcga taaaactggc acattaacga
    11101 aaggtgactt ttcgatccag acgatcgatc caacaggaat tgatccaaag aaatttatgc
    11161 aatatgttgc aagtgcagaa cagttttcta cacatccgat tgctcaatct gttttagaag
    11221 gctacgcagg tccgctttta ccaacagcta atatccagga atttgcaggt gaagggatct
    11281 tagctgaagt tgatggcaaa caagtattag taggtaatca caaattgatg gaaagatttg
    11341 aaatctcttt tccttcaagt caagaaatcg gtacattgct ttatctagcg atcgatcaaa
    11401 gttacagtgg ttatcttgtt attgcagata ccttgaaaga agatgccgta gacgcattgg
    11461 ttcaattaaa acaggctggt gtgaaaaaca cagtcatgtt gacgggcgac tcgaaaaaaa
    11521 ttgctgatca tatcgggaag caagtaggcg tggacaagat atatagcgaa ttgcttccag
    11581 aagataaagt gcagcggtta gaggaaatct tacaaagtaa caataaaaag accgcatttg
    11641 tcggagatgg aatcaacgat gcaccggtgc tagcaagagc agatgtagga atcgcaatgg
    11701 gtggattggg aagcgatgct gcaattgaag cagcagatgt agtcatcatg aatgatcaac
    11761 catcgaagat tgcagaagca atccatttag caaagaaaac attgaaaatc gtcaaacaga
    11821 acattgtctt tgctattggg ataaaaattt tggtactttc cttaggtgct tttggttttg
    11881 catcaatggg agatgcagta tttgcagatg ttggggtgac agtattagct gttttgaatg
    11941 ccatgcgaag cttgcatgta aaaaataaac gttgatcaat gaagaggacg aatgaacgtc
    12001 ctcttttttt tatatccttt tcttaatgaa aaatgtaaag aaaatagata ggattataaa
    12061 ataactaatg tttttaattt tgcagaaaat tcaaaatagc ttgaaattca ataaggtcaa
    12121 tgaaataaaa gaaaataact tatttttttg atggaaaaaa tatgaaaaaa cgatgtgtat
    12181 taaataaaag aatacacaca taaaataaca ctgtcttgta agtttgttaa aaattgaata
    12241 tgattacatc tggataacaa atttttatca taaaggagag aacgaatgaa caacaaacaa
    12301 ttacgctggt ttactgttgc gttaattgca tttaacatgg tttggggcat ggggaatgta
    12361 gttaataact atgctcagca aggagtatca gttgtcacct cttggctatt gattttggcc
    12421 atttatttta ttccatacgc attgatcgtt ggacaattag gatcagcctt caaggatagt
    12481 aaaggaggcg tcagttcatg ggtcgaaaat acgacatcta ataaacgact ggcatactat
    12541 gccgcttgga cttactgggt cgtccatatt ccttatttgg cacaaaagcc tcaagcaatt
    12601 ctgattgctg gtggttgggc agtccaagga aatggaaata tcgtaaatac gatgtcagta
    12661 caaatggtag cagttatttc acttattata tttcttgcct ttttatattt atcaacaaaa
    12721 ggactttcta ctttgaaagt gattggcgga ctggccggaa cagcgatgtt cgtcatgtcg
    12781 atcttattta ttcttttagc tgtaacggca ccgttcatca atccaacgat ggaatttgcc
    12841 acaccacata tgaatcaagt gaaaacatat attccaacat ttgatttcag ttatttcaca
    12901 accgtttcga tgctagtatt tgcagttgga ggggcggaaa aaatatcccc ttatgtcaac
    12961 tctacaagaa atcctgcaaa ggaatttcct aaagggatga tcttcttagc agcaatggta
    13021 gggatttgtg ctgtattagg ctcagtcgca atgggtatgc tgtttgctag cgataatatc
    13081 cctaaagact tgatggcaaa tggggcatac agtgcttttc aaattttagg taattactat
    13141 ggtatcggta actcattgat gatcatttac gcattgacaa acatgattgg acaaatctcc
    13201 gcattagcct tttcaattga tgcaccacta aaaatcttgt tagcagatgc ggatccagaa
    13261 tttgtaccaa actggctacg taaacgtaat cgtaaaggta cattggtgaa tggttatctt
    13321 ctgacaggta tactagtagg aatcattatt ttgttgccga tctttggtat caaagatatg
    13381 aatgaattag taaaatggtt gacgaacttg aactcagttg tcatgccaat gcgttaccta
    13441 tgggtgttct tggcatatat gatgttgaat aaagcttata agaaattcaa ttctgaatat
    13501 aaatttgtta aaaatccaaa aatcggtttt atctttggtg cttggtgctt tgccttcact
    13561 gcgtttgcat gtatcctagg aatggtgcca aaagtagatt atactgctga tcctaaagca
    13621 tggtggttcc aattattcac gaatatcatc acaccaattg tcttgatctt attaggtatg
    13681 atcttgccag caattgctcg tcgagacaaa aacaaagaaa ttactgaacc agaaacagtg
    13741 gttcagcctt aaaatagaaa tacttgttac aagaggttgt gacagaagct acttctggaa
    13801 caccgtgaat cagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac
    13861 aaaaatagct cctcatattt tgccgttttt tgagctaatg ctgaaggtca gctgattgaa
    13921 caccatttat tcgaaacaag ggactttgac acgacttttg tcacagtccc ttgtttt
//
LOCUS       DAZKOW010000076        13879 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000076 DAZKOW010000000
VERSION     DAZKOW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.076"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            153..1016
                     /locus_tag="ACG912_002100"
     CDS             153..1016
                     /locus_tag="ACG912_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFQ0513399.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1145..2767)
                     /locus_tag="ACG912_002101"
     CDS             complement(1145..2767)
                     /locus_tag="ACG912_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFQ0513400.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3016..3537
                     /locus_tag="ACG912_002102"
     CDS             3016..3537
                     /locus_tag="ACG912_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0513401.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3723..5453)
                     /gene="spxB"
                     /locus_tag="ACG912_002103"
     CDS             complement(3723..5453)
                     /gene="spxB"
                     /locus_tag="ACG912_002103"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFQ0513402.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5618..6436
                     /locus_tag="ACG912_002104"
     CDS             5618..6436
                     /locus_tag="ACG912_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFQ0513403.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6539..6736)
                     /locus_tag="ACG912_002105"
     CDS             complement(6539..6736)
                     /locus_tag="ACG912_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513404.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6905..7483
                     /locus_tag="ACG912_002106"
     CDS             6905..7483
                     /locus_tag="ACG912_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFQ0513405.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7578..7715
                     /locus_tag="ACG912_002107"
     CDS             7578..7715
                     /locus_tag="ACG912_002107"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513406.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8528..9031)
                     /locus_tag="ACG912_002108"
     CDS             complement(8528..9031)
                     /locus_tag="ACG912_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0513407.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9390..10415
                     /locus_tag="ACG912_002109"
     CDS             9390..10415
                     /locus_tag="ACG912_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0513408.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10738..11616
                     /locus_tag="ACG912_002110"
     CDS             10738..11616
                     /locus_tag="ACG912_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFQ0513409.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12092..12208
                     /locus_tag="ACG912_002111"
     CDS             12092..12208
                     /locus_tag="ACG912_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0513410.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13136..13474
                     /locus_tag="ACG912_002112"
     CDS             13136..13474
                     /locus_tag="ACG912_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513411.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13568..13798
                     /locus_tag="ACG912_002113"
     CDS             13568..13798
                     /locus_tag="ACG912_002113"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFQ0513412.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4407 a         2300 c         2660 g         4512 t
ORIGIN      
        1 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcttatttt gattagaact
       61 tgcgaaaata aaccggttaa agtgtgtcta gcgcaagaga tttgctataa tagacgataa
      121 gggtaaaatg taacgagaag gggttgaaaa atgtgtatca aattgtaaca gattcttgtt
      181 gtgatctgcc ataccatgta ttagcaaaag aaaaagttga ttttttatca atgaatctcc
      241 aattgaatga tcaagagtta gtagatgacc tcggaaagac attcaattat gaccgctaca
      301 tgaatcagct gaaaaatggt gcgatgccta cgacatcaca aatcaatgtt ggccgatatt
      361 tagaattttt ccgttcttat gcggaaaaac agattcctgt tttatatgtg gctttttcca
      421 gtggattaag cggctcttac agtagtgcgc ttcaagcggt tgaattgctg aaggaagaat
      481 atgaagaccc tgagatctat gtttttgata caaaagctgc aagtattggg gaagggcttt
      541 tagtattgga agctgtaaga ttgaaaaaag aaggtaaaag catcgaagaa accctagagt
      601 ggctggaaag taattatttg aaagtccatt catgggtaac agtagacgat ctgaaatatc
      661 tagaacgagg cggtaggatt tccaaagcaa gtgctactct tggcagtcta ttgagtgtaa
      721 agcctattat tacggtggat aaagaaggaa ggctgacgaa tgttgacaaa gttcgaggaa
      781 gaaacaagtc gatccagaca atcgtcaatg agacggtaaa aacaatcgtt gcaccacttg
      841 atcaaatcgt ttatgtggct tacgctgggg atctagagag cgcggagaag gcaaaacggc
      901 ttttggaaga gcagataaag atgaaagatg tgaagcttta tcccttagga ccaacgatcg
      961 ccagtcacac aggctatggc tgtatcgcga ttttttcgat gggaatcagt cgataaaagc
     1021 agtaactcat tctgtaaaaa agcccagaaa ttgattgtaa caaaaacagc gactcttaaa
     1081 agatagatga gcatcctatc tcttagaggt cgctgttttt ttatttttta agctaaatag
     1141 gccattatgc ccataactcg ttcaattgtt tttgaatatc tgcattttcc aaaaattcat
     1201 catatgtcgt atcagctcga tcgatcacac ctttatccga aacggcaatg atgcggtttg
     1261 ccaatgtttg gataaattga tggtcatgtg acgcaaataa gattgagcca gtaaatgcca
     1321 tcaagccgtc attcaatgct gtgattgatt ctaagtctaa atggttcgtt ggatcatcta
     1381 atacaagtac gttagctttc gaaagcatca tttttgaaag catgcaacgg actttctctc
     1441 caccagataa aacattcact ggtttcaata cttcttcacc agaaaacaac attcttccta
     1501 agaagcttct taagaaggtg ttatcatctt cttctttact tgcatattga cgcaaccagt
     1561 ctaaaatcgt tagatcagta tcgaattctt tcgttgtatc ctttggtaaa taagaattac
     1621 ttgttgtcac accccaacgt actgttccag tatctggcgt aatttcaccc atgataactt
     1681 tgaatagcgt tgttgttgtt atatcatttt ctgcgacgaa agcgaccttg tcttctttgc
     1741 tcaaattaaa agatatatca tctaaaattt tctttccatc aatcgttaca cttacatttt
     1801 ctacctgcaa taagtcgttt ccaatctcac gttcaggttt gaatccaacg aatggataac
     1861 gtctagaaga aggttggata tcgtctaaag taattttttc aagcattttt ttacgtgaag
     1921 ttgcttgttt tgattttgat gcattcgcac taaagcgggc aataaattct tggagttctt
     1981 tgatttgttc ttcttttttg gcatttgcgt ttgcttgaag tttagccgct agttgactag
     2041 attccaacca aaaatcataa tttcctacat aaagtttgat tttaccaaaa tctagatccg
     2101 ccatatgtgt acatactttg ttcaaaaagt gacggtcatg ggatacaacg atcacagtat
     2161 tttcaaaatt gatcaaaaat tcttccagcc aattgatcga ttggatatcc agaccgtttg
     2221 ttggttcgtc tagcaaaaga acatcaggtt taccaaatag tgtttgtgct aataacactt
     2281 ttaccttttg tccaccagtt aattcactca tttgctgatg gtgcaattct tcagggatgt
     2341 tcagaccttg cagtaaaaca gctgcttctg gttcagcttc ccatccatta agctcagcaa
     2401 attctccttc taattcagct gctcgaatac catcttcgtc agtgaaatcc tctttcatgt
     2461 aaatcgcatc tttttctttc agcacttcat acagacgctt gtgtcccatg atgactgttt
     2521 caagtacagt atagtcttca tagtcgtaat ggttctgttt caatacggcc atccgttcat
     2581 ctggtcccat agtaacggaa cctgtggaag gctgcagatc ccctgataac actttaagga
     2641 acgttgattt acctgcacca tttgcaccaa tcaagccata gcagtttccg ggggtaaatt
     2701 taatattcac atcatcaaat agtttgcggt ctgaaaagtg caaactcaca tcatttacag
     2761 taatcaattt ttttcctcac ttttatcatt attagttttg acaagtctta tagaattata
     2821 taggggaaag gtaaaagatt caaggcaatc gaacattctt tgtgtttcac gtgaaacaga
     2881 atgaaaaaaa taaaaataaa agcggagcga aaatagctac tataggattt attagtttgc
     2941 aacagcataa tttttgacgt tatttataag agggagtact ataaaactgt aaaagaaatg
     3001 aaagaaggat tgaaaatgga tgaaaaaaga accatcgact ggggctcatt gattttggga
     3061 attttatttg tacttgtctc attgttatca tttcaagacc ctgtcggaaa tttagttgca
     3121 attgtagtag tctttgcgat ttttgccttt attaaaggga tatttgaact gtttgtacgg
     3181 aacagattaa aagagctaac aggatataaa ggaaagatgc ccttaataat cgggatcatc
     3241 gatattctag ttggcgtctt tttcttattc aatattggtg caggagtcgc agcattgcct
     3301 tttgtttttg cagtatggtt tattgctgat tcagttttag cgttatttac tgctgacttg
     3361 gcaagaggag taagtgaagg atattattgg tttacgatca tcgtcaatat tttaggaatt
     3421 ttattaggaa ttttcttatt gtttaatcca atttcttctg cgttgacatt aagcttttta
     3481 gttggattct attttatgtt atttggtatc acacatatcg tctatgcatt tagataagta
     3541 aaatcaaaca aataaaaaag ccggcttccg attacagttt tactgttgta attggaagcc
     3601 ggctttttta tcatggacaa atatgcacga ggtgattttt aggtgtcgag acaaatttat
     3661 gcaaaaatgt gagaagctat atacacaaat tttatcttaa tactcgaagc atagcgcagt
     3721 tgtcacaacc tcttttcata taattcattc aatactggca aatcatggac ttcatatttt
     3781 tctttgaatg cttggatttc ttcttcagaa tattttcttg gatctaaatg cagatcttct
     3841 actggcaatg ggcgcttgtt attgatcttc acatcgatta ctacaggacg atctgatttt
     3901 gcagctgcat caaaggcaaa tcgaagttgc gagtattctg tgactgtaaa gctatttgcc
     3961 ccgagcgctt ctccaacttt accgaaatct gcatcttcta aaaatacacc aaatttcttt
     4021 tgttccgtat cttcttgttc tgcttcgatg aaaccaaaag aatcattcga aaagacaaca
     4081 ttaataattg gaagctgata tttgacttgt gtaatgatat cttgcatgac catagcaaac
     4141 ccgccatcac cacttaaggt aaatacttgt ttttctgggt agcttagttg tgcggcaatt
     4201 cctcctggca caccattacc catagttgcg aaccatccag atgtcgtatg tttttgttta
     4261 ccgttcatat tcaataaacg aatagaatgg atagtcgtgt taccgacatc tgtgacgaaa
     4321 atcgcatcgt cttcagcaat acgattgatt tctttataga cagcctctgg acgaatcggt
     4381 tcttcctctc gatcttcaaa agatttcaac caatttaccc atttttcttt attttcttga
     4441 tttgctaggt accagctatc agcagggcga gcatccccta actcgacaag tctttgcaaa
     4501 gctgtgtttg catcaccgag gatcgacaaa gatgtttcat ggcgtcgtcc aaattttgac
     4561 gcatcgatat cgatttggat aaattcagca gttggattaa aaaatgcacg tccaaatggg
     4621 aagtcgctac cgacaaataa gatcagatcg gcttctgcca atgcttcatt tccgggttta
     4681 gtagcgacac gtccagcaaa accaagaaag ttttcataat catcaggaat aattcctttt
     4741 gctagaacgg aagcgacaac aggaactgaa aaatgttctg agaattcttt gatttgcgga
     4801 aatccgtttc gcgttccttg accaacataa agaactggtt tttttgcttt ttcaagataa
     4861 ggcaaagcag ctagcaaatc tttttcttct ggtaatatca cacttgtttt gtgagtgtga
     4921 gcggtggaga aaggttgttc gtcaatttct tcaaaaccaa gatcaacagg gatagtcacg
     4981 atagccacgc ctttatgctc ataggcggct ttaatggctt catctactac atgcggaaga
     5041 ctttgtggtg tcatgactgt acgattgtat acgctaacat ctgcgaaaat aggattttca
     5101 tttagttcct ggaaagagtt atagttcatg gatgtagaag caacttgtcc taagaacgct
     5161 actagaggca catggtccat ctgtgcatca tacaaaccat tgatcaaatg tgtcgcacca
     5221 ggtcctgcag agccgaagac agcgccgact ttccccgtta attttgcatc tgcagcggct
     5281 gcgagtgcgc cgacttcttc atgacgaact tgaatgtagt tgatttcggt tttttcttga
     5341 tatagcgcat ccataattga attgaaagat cctccgggaa tgccgtaaat atggtcgata
     5401 ccccagcttt caagaacttt taccattgcg attcctgcat tgatttttga catggtgttt
     5461 cactccttta gcaatgatga tacataaatt ttttgtatct agagtataga actttttatc
     5521 gaaaatccca actaattcgt cttttcacaa aaatgtagtc atgtttttgg gaatatgcta
     5581 gagtagtaca tagatagatt caagagaggg gaatggcatg gttgaatggc tattgagact
     5641 tgaaccttgg cagcaagcac ttgctgggac agcttttact tattttatga cagctcttgg
     5701 tgccgggttg gtttttttct ttaaagaaat caaaaaagaa gtgctaaact tgatgttagg
     5761 atttgcttct ggtgtcatga ttgctgcaag tttttggtct ttgttagatc cggctattac
     5821 aaaagcagaa gaaaatggag atattgcttg gctagttgta agtattgggt ttggtttagg
     5881 cggtgtgttt ttatatatgg cagataaaac actgccccat atgcattttg gaccacaaca
     5941 tgaagcagaa ggattgccta cccatttaaa acggacgatc ttgcttgttt tttctattac
     6001 attgcataac attcctgaag gtttagccgt gggggtagca ttcggtgcag cagcaactgc
     6061 ggataatccg acagccgcta tattagccgc tgtttcggtg gcgctaggta ttggtatcca
     6121 aaacttccca gaaggagcag ctgtatcaat cccgttgcga caagaaggtc tgagtcgtaa
     6181 aaaggccttt gtttacggac aagcttctgg aatcgttgaa ccaattgctg gagtgattgg
     6241 agcgctacta gtaacgaaag tggagctgct gcttccttat gcacttgctt ttgccgcagg
     6301 agcaatgatt tatgtcgtgg tggaagaatt gatccctgaa gcacagcaga cattgtccag
     6361 caaacgtcat tttgcagttt ttggcgtcat gagtggattt atcattatga tgattttgga
     6421 tgtggcatta ggataaataa aaaaaatata tatgatatat caaatagggc cgcgaactta
     6481 tatcgcgacc ctattttttt agtaagttat atcataatga agtgaatggt atcatacatt
     6541 aataatagtt ttttctatgt gtatttcctt tgatccagcg aacaagccag atgataagtg
     6601 cgattgggat tagaactttg accgcaaacc ataaaagccc gcttaggaca ctgacaaaaa
     6661 gactgatgac gaagctagct gcaattagca ctaaaagaac catgaagata tttaagaaca
     6721 tatttttttg acgcatacaa aaccctcctc ttgttcattc tttactatag aaaaagtata
     6781 acagggattg tttaaaaaca cctccaactg aagactgatt tttacttcat ctacttaaat
     6841 gatcaaacaa ttgctttttc tctggtttat catctaaaat agagcggaaa aggaggagaa
     6901 tatcatgcct aaaaatcgtc ttgaagcatt tacagatgct gtaatcgcga ttatcatgac
     6961 tattttagtt ttagaacttc atgaacctaa cggagacacg tgggaggcgc tcgctgcatt
     7021 aggttaccga ttttttatct atattgtcag ttttatcatt ttagcgattt attggaacaa
     7081 tcatcatcat ttatttcaag ttgtacataa gataaatgga cgggtcttat gggcgaacag
     7141 tttctttgtt tttgcgcttt cgctttttcc gtttgcaact tcatggatca gtgagtacat
     7201 caatagcctg gctccagaaa tgacttatgg ttttgttacc ttgaccgcta atattgcgta
     7261 ttatatcttg gcaaaagaac ttgtcaaagc aaataaaggc cacgagaaag cattgaaggc
     7321 gatgttgaat aattacaaga aatcgtatct atcgattggt ttgaatgtgt taggaattgt
     7381 cttagggtat ttagtagcgc cagtagccgt gctgatcgca aacatcgtga ttctagctgc
     7441 ttggatcatc ccaagtcgta ccatcgaatc acaatatcgg taaaatgatc cgtgtttaaa
     7501 atttaagaga aaaatataga aaaaataaat aaacgatcaa atatccgagt gatactagta
     7561 ttcaaagagg gatgcagatg gatcaaaaaa gaattcatgt aggactgatt actagttttg
     7621 tcacgatgct tatttatttg gatcaaggtc atttgacttg gacagtagtt ggtgtcgtag
     7681 gatttttgac attattcaca gcaataaaaa aataaacatc tttatgtaat ctgatgttct
     7741 atctatttaa gaaaagcatg tttacagcag tatgattctg ttgtaaacgt gtttttattt
     7801 gtggaaaatt taatttttag aaaagtgtat cgaaaaatag atagttagtt tttccacagt
     7861 ggataacttc gtaagagata aaaataaaaa attagtcaaa gaagggcgta tagatttctt
     7921 ttgataggag atatacaaca gaaatgaata aatattaacg tgttttcaaa cagtataaaa
     7981 cggtgtggtt tttggaataa aaaaataaca tatctcattt tacacttttg ttattttata
     8041 cttaaaaaat aacgacacag aaaataccgt gtctcagatt tctctactac ttctaaaaac
     8101 tactgttttt ttcctttgaa atgccttcaa aaagtaggat caggcagaat atactggcag
     8161 aatcaaggag aagggagcct aaataaggag aaaaaccata gaaaatataa agtaattact
     8221 tataaaacaa gctttttata gaaaaataat atacctgagt agggtataaa ttaagacgga
     8281 attcctttct agattgagat attgactagg aagaatgtta agattttaag cctttaagct
     8341 aaaaaaaaac cctttttgtt aaataagggg aatatagcct gtcagtttca aactgcaagt
     8401 cgtcctgctg caataatata gtagattaga gatgaaaata gattttttta aaccaatgac
     8461 tgaataaaaa gaccgtcata gatacacatt ggaatgtgtc tattacggtc tttttttacg
     8521 aacgatttta ttgaagacgg acagttcggg tcaaaaataa aaaagctacg atcagaaata
     8581 agaataaaat gtatcccaaa ataatggcta gacggtctaa cttatttgtc gatagatgtt
     8641 tgtttagaag ttgataggcc cataaaccta tcagtcctcc tgttgtatta gttatcaaat
     8701 cagtgatgtc cgctacgcca ataccgaata ggaattgaat caattctgcc aagaagctta
     8761 caaggaaaat aatccctaat tttttagaaa aagaaagtct cttgaaattg acattcaaaa
     8821 gcaccccaaa aggaaaaaag acaatcaagt tataagtggt ctcacgtaat accgtcgtat
     8881 tttcaaaaga aaaagtagaa aaagggacga gattgacatt gctgtaagaa taagtgagaa
     8941 attgtggaag attaaatgat agcttgaata aaatcaacca taatagaaag aataaataaa
     9001 tccctaataa aaattttgat acataccgca tagacatctc ctccatttaa aaagtatacc
     9061 atcgaaaacc gctgaaaaat cataaaaaag tatgaagttt aaggaccaac gtagttatcg
     9121 tacgaaaatt agaagctttt ttttgttggg aatccggttt ctttaacaaa gctaagatag
     9181 cgagttgctt ggaaaagttg ctatcttttt tctgtcaaaa cgactcggaa tgttcgtttt
     9241 ttgttctaat ttctccaagt tgtttacttt ttgatactaa gtttatgaaa aatcaagaaa
     9301 tagttgttat actaggtaat gctgacaaac gggattggca tttcatcttg gttcagcttt
     9361 ttttccaatt actaggaagg agggatagat tgttcaagac ttattttcgt ttcaataaaa
     9421 tacaagataa tccttttcgt atcatgaatc tgatgctact tatctttatt gtattatggt
     9481 taggttacat aaatcatgat tttttgattt caagtttttc atcattaggg atctttactt
     9541 ttttctatta tcaaaacata ccgatgaaat atttgctgaa acgtatgttt ctgatcggtt
     9601 gcggactgtt ggttgctttc gttttgggga tcttatcgac ttatgtttta tggcttgaac
     9661 catttgccgt agctttggtt gctttttcca gcagattcgt gttgcgatta ttccatattt
     9721 ctaaaccagg tggcttgttt tttgcgatgc tttctgccat gggaacgagc atgcagctgc
     9781 caatcgctca attaccgatc gtctcacttt atttttttat gggtgtcgtt tttgccctga
     9841 ttgcggcagt gattacaaaa ttactggatt cacggcctga gcagacaatt gaaaaagcaa
     9901 ctttaaaaga aagatttcac gaagagcctt tagtgatcat tgattctgtt ttctacagcg
     9961 ctgccttatt tctttccgtt tatgtcagtc acggcttgaa tctgcataat ccttattggt
    10021 tgaccttgtc atgtgcatcg attttgctag cagaaaactt ggatgcgatg aagcatcgac
    10081 aagtccaata tctgattgga tcgatgggtg ggttatgtgt gtcggcattc ttgtcatttg
    10141 tgccgtttac gcagttgcag accatttttc tgatcacttt tctatatgga atcgcacaat
    10201 ttctagttgc tagaaattat gcagttgcta atatttttct taatccgatg gcgttgatgc
    10261 tatctacact tattcgtgga gcatacttga tttctttgat cgaatatcgt tttttaggga
    10321 tcgtcatagg aagtttcatc ggtttaggtg ttgcgtgggt aatgacggtt ggtttgcaac
    10381 attatttaac ggttgttcgt caaaaattag aatagaagga caaatatatg tgccaaaaga
    10441 ggttatgaca agatttttgt cacaacctct aattcaaggt gttcaaaagc cagattctca
    10501 gcattaagtc aaattcacac aaaaatgtgg aaaggaactt tcaaaaaaat tttatattta
    10561 ttctatcaaa aaattttgtt tatgatggta actgaataaa ctttcctgag atggtcaatg
    10621 ctttccttga aagtcttaca gatattcttt cttcatagaa agtttaagtt ttatagttag
    10681 tgtacaataa aagtatgttt ataccatatg gtgtccggat aatcagtcat ttaaacaatg
    10741 aggttcaaag atatgaaaag gggaaatttg atgaagaaaa taagcaatga aatccaaatt
    10801 ctggcagaaa ctttaactct tcccaaagtc gttatagagc aaatgattgt acaagcaaaa
    10861 aacatccttg atgaggtgtt tgagcaatat agtcatgaac taatggatcc taataaagca
    10921 aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc agcattagct
    10981 atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt gaagattcca
    11041 agaactatct atttggcaac aatgaatgct tttactagat ttattcatga acatttccaa
    11101 gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac tagtggcttg
    11161 atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg gtacacggat
    11221 ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc agatttatct
    11281 cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact ttattttcct
    11341 gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa tcttactaaa
    11401 tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt attatcagtt
    11461 gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc ccaaatacat
    11521 gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct tgcaggcgag
    11581 cacattggct ctggcttagg tattcttact tcataagttt agtacttcga aatagtttaa
    11641 aagagcgtga gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    11701 cggaagcaga agcaactcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat
    11761 tttcgtgaat ttcttcttat ttcttggagt taaacgcttc tgtcccagcc tcttttttag
    11821 tgttctatcg aacacacagt acgttcaata ggtaaactag ctataacaat agatattaaa
    11881 gttaacggac aagagggcct tttttaggta gctaaaacgt aataatgaaa atttatttcc
    11941 agagatagga gtcaatcagc tagtctgatc gactgtttta acaagttgac tttcatttta
    12001 acttttgata ccatttaaaa cacaaatcgt aattgtttcg atttgtgttt tttagcatga
    12061 ctacaatcaa agaagaaagt gggtcgtcca tatggaaaaa acagatatct caagtgcata
    12121 ccgccgcttg aaaagcccaa atatcaaaac acgaaaacgt gcgctgaaga tcatcaaaga
    12181 tgtgaaacgg aatagtggga aaaggtaaga agaaggaaac ttaattaaaa aaatagccga
    12241 aaaacgtttt tcggctattt tcaataatcg gtttttgtgt aaggtttccg aaagaactgc
    12301 tgttgatttt ccatttttaa catatgataa attggtagaa tctttctttt caaattgata
    12361 ccattcccta attccagtta tatagttaac tacagaacca caaagcacgt acgtttataa
    12421 aagtattatc tgtagttatt tttcaattcc ctttcaacct cttattttga ccagtagtat
    12481 tgatcacttt accttttaac acctttgttc gtttttgaaa catttttagt caagaatgga
    12541 aacgtaacta cataatcaaa ggttttgcga aatatttcat gaaatatttc atgtagaagc
    12601 gagcaatacg gttgcaaata accattatcg tttaataagc agttacgaaa gctcccaata
    12661 tctctaacgt tcagctcact ccatttcttt tcttcaaaaa aatcagtgat tcgtatctct
    12721 ttctatttgt cgaaaaagtt aactttaaaa agtaacgccg aaaaaatatg caaaaaaata
    12781 aaaaagcagt tactattgct agaataacat caaggaataa taagttttgg ttttcagata
    12841 atagtcgata agttgtgtat tggaatttta gatacagtaa taaagtattt ttcatgaaat
    12901 tttgtgagaa tcactattac aagtatcttt aataaaatta atggttaagg aggaacttaa
    12961 agtagaatgt aaatgaatag ttgtagtcag tcgattgtga tactagactc aaagttgcag
    13021 gaataagttt ttcaatttgt gattggaaga taaaaaatga aaaagaaggg ctttttttta
    13081 tactttgttg attgtagtgt ttattttccc tctgtttagc caagtaacat tttctatgac
    13141 tcaaccagtt tcaagtgagg aacatgataa cctttttcgt gctacagaag agtttaagaa
    13201 atatcaggaa aatattgttc cggagactcc attagctagt gagccaatta ctgatattga
    13261 tggaaaccaa ataggatggt atatacagta cgaagttatg gtggtaaata atgactcaat
    13321 agtaaaaaat acagtaggct tagagctaac accgattgaa aaggtgagtt cattgttaac
    13381 gtatatttat actgatgaaa gtgacgagac tgagatcatt ttaacggatt actcaaaaat
    13441 tgctactgat aaaaagtata tattgtggat gtagacacta gtgaaatact ttatgatata
    13501 gaaattgatg aaaatttact agatgtagca caagaaatcg atcatcaaaa gcaggaagct
    13561 attcgatatg ctggtagaaa agggaaaaga aaatatttat tacgtgaatg tagcaaaagt
    13621 tagagaagat gaaaatgagt ggaaagaatt caagagtcgc tactcaatta actcgactcc
    13681 gacatttaca gtttacagag aaggatcgat agaaaaaact gttttttgga ctaaagaaag
    13741 tggaatgtct ttagctgaag tagaagagtt tttggattat gttagtatgc aacagtaagg
    13801 gagttcttat tatgaaaaat tttgacacat attatgttat tgttttttcg atatctttgc
    13861 tgttaatatt actaacttt
//
LOCUS       DAZKOW010000077        13807 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000077 DAZKOW010000000
VERSION     DAZKOW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.077"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG912_002114"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="ACG912_002114"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFQ0513413.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="ACG912_002115"
     CDS             complement(2260..2511)
                     /locus_tag="ACG912_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFQ0513414.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="ACG912_002116"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="ACG912_002116"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFQ0513415.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="ACG912_002117"
     CDS             3461..3679
                     /locus_tag="ACG912_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513416.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="ACG912_002118"
     CDS             complement(3968..4480)
                     /locus_tag="ACG912_002118"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFQ0513417.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG912_002119"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="ACG912_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFQ0513418.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="ACG912_002120"
     CDS             complement(6933..7376)
                     /locus_tag="ACG912_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFQ0513419.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="ACG912_002121"
     CDS             complement(7377..8165)
                     /locus_tag="ACG912_002121"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0513420.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG912_002122"
     CDS             complement(8162..9103)
                     /gene="rnz"
                     /locus_tag="ACG912_002122"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFQ0513421.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="ACG912_002123"
     CDS             complement(9280..9867)
                     /locus_tag="ACG912_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFQ0513422.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG912_002124"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="ACG912_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFQ0513423.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="ACG912_002125"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="ACG912_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFQ0513424.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="ACG912_002126"
     CDS             complement(12784..13719)
                     /locus_tag="ACG912_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0513425.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3095 c         2435 g         4469 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataac
//
LOCUS       DAZKOW010000078        13615 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000078 DAZKOW010000000
VERSION     DAZKOW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.078"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..144)
                     /locus_tag="ACG912_002127"
                     /pseudo
     CDS             complement(1..144)
                     /locus_tag="ACG912_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(186..1312)
                     /locus_tag="ACG912_002128"
     CDS             complement(join(186..1084,1084..1312))
                     /locus_tag="ACG912_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0513426.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            complement(1716..2498)
                     /locus_tag="ACG912_002129"
     CDS             complement(1716..2498)
                     /locus_tag="ACG912_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513427.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(2563..3399)
                     /locus_tag="ACG912_002130"
     CDS             complement(2563..3399)
                     /locus_tag="ACG912_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0513428.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3417..4826)
                     /locus_tag="ACG912_002131"
     CDS             complement(3417..4826)
                     /locus_tag="ACG912_002131"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0513429.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4840..6663)
                     /locus_tag="ACG912_002132"
     CDS             complement(4840..6663)
                     /locus_tag="ACG912_002132"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFQ0513430.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(7506..7832)
                     /locus_tag="ACG912_002133"
     CDS             complement(7506..7832)
                     /locus_tag="ACG912_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513431.1"
                     /translation="MNKVNMNKKFIIIVIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7878..8240)
                     /locus_tag="ACG912_002134"
     CDS             complement(7878..8240)
                     /locus_tag="ACG912_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFQ0513432.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVALAFIPVVFGAVAVIMGYLYRDKDEQMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(8277..8491)
                     /locus_tag="ACG912_002135"
                     /pseudo
     CDS             complement(8277..8491)
                     /locus_tag="ACG912_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            8619..8954
                     /locus_tag="ACG912_002136"
     CDS             8619..8954
                     /locus_tag="ACG912_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0513433.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9030..11027)
                     /locus_tag="ACG912_002137"
     CDS             complement(9030..11027)
                     /locus_tag="ACG912_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513434.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSFYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELTNTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKRLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(11125..11400)
                     /locus_tag="ACG912_002138"
     CDS             complement(11125..11400)
                     /locus_tag="ACG912_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513435.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11632..12366
                     /locus_tag="ACG912_002139"
     CDS             11632..12366
                     /locus_tag="ACG912_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0513436.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12405..13547
                     /locus_tag="ACG912_002140"
     CDS             12405..13547
                     /locus_tag="ACG912_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0513437.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         4373 a         2681 c         2089 g         4472 t
ORIGIN      
        1 ctccagataa gtgcgatact cagacgcatc attcagtaac aaccgaacgg ttgtcgtccc
       61 ccaacgaatg atcctagagg ggacgacaca aagacaatgg gaacactctt ctggcttgta
      121 cagaagagtt cctttgtaaa tcatggattt gagtcaatat cttggacaca ttttagtaga
      181 gacggttaag ccattttttc ttttgctaat tcttcaaatt cctgtggggt tagatagtga
      241 atcgagctat ggattcgatt tcggttataa aatccttcaa tgtagctaaa tagcgctcga
      301 tttgcttctt caaaatctga gtagctagtc gtgtagactt cttctttttt caatgaggca
      361 tggaaagatt cgattccagc attatcataa ggtgttcctt ttctactata agaatgcctt
      421 attttgttgg ttttaagcca ttgttccact tctcttgctg tgtattggct gcccaagtca
      481 gtgtgtagaa tcattccttc tggaatgtga taatgtattt ttgctttatt caatgtttga
      541 atgacacaat ccacagtcat tcgttttgaa aatgtataac taataatttt tttcgtgtgt
      601 aaatccatga ttgaagataa gtaacaccaa ccatttttct tagtaggaat gtacgtaatg
      661 tcagctgccc atttctcaca gatcgtttca gtagaaaagt cctgatttaa gagattctct
      721 tttgaaatga tcggtttatt agacctttga ggtctatatt ttttaactac aatcgacctt
      781 aaatttaatt gtttcattag cttttggaca tgtttaagag atactgaaat cccttctttt
      841 aataaaactt gatggatctt tgtggcacca tagcgttgtt tattcgtaaa aaaaattgtt
      901 gaaatcttct ttttcaactt attatttctg atttctgtag ctgtcaacgg tttattctta
      961 taaaaataat agacacttct agggatttct aataaccgac ataacaaaga aacagtgtaa
     1021 tccttacacc atttttcaat aaattgaaca agcgaattta ggttttccga gagaatatgg
     1081 ctgcggcttt ttttaagatt tcaagttcct catttaaacg agcattttct tttctcagtt
     1141 cagctacttc ttttccagtc agtcctgtgg actgatcagg taaatataaa ttcttccatt
     1201 tgtaaatcgt tgcttcagat aagccatatt ctttcgctaa ccctcgaaca gaacgtccag
     1261 tttgattcag ttcaacaatc atttgtttaa aattttcttc atatcgggtc ataaaaattc
     1321 atcctctcgt agataagttt aatttattct tgtctctacg aaaagctgtc taagatttga
     1381 tactaacacc aagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
     1441 tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc ttaaaaattt
     1501 tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg tttaatgaaa
     1561 ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa
     1621 tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat ataacaacgg
     1681 ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt tttaaatact
     1741 tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt tttcccattt
     1801 tcaagtaacc gaagtggaat gtctttacca acttgctctg tcactgcatt actgatacct
     1861 gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc tgtatcagta
     1921 tttatcgtga ttcctttatc ttcatttaca agcttccaag taggtggtat agctgtgtat
     1981 gtttcgatga atgaatcaat tctttcatct atcgtcttcc cagtatctac ttctgtctca
     2041 ttactcttag atgaatcgat tctttcatcc atcgtctgct ccgtaactgc ttctgtctca
     2101 ttattctcac cggaaactct cttcacaccc aaacttatat attgtccatc tttagtgtgc
     2161 atagttaaaa ttcccacgct tttactaata aattcactat aaccagttct ttcaatagag
     2221 acttcaatgt atttcacact atcatactca aaagaaaaat agacatgtaa aagcattttg
     2281 ttgtttacat ctacataata atcctttgac tctacttgag accttcctat acggaataca
     2341 gctttactgt ctgatatctc tataccagca tccttattat taatctgcca atctccaagt
     2401 agctcttctg gcaagctagt agtttcttgt gaacacttta ccacttcact ggtagaggtc
     2461 gaaacattca cctccgcact gttagaagaa ttttccaagg aatcaggagc cttagcacaa
     2521 ccaacaagtc tttatatatt gtgcagccga tattttcttc attcagtaca cccttgaaat
     2581 atgcagtgtt agataaagca gttcgtcctg atttgctgag gcacccatct cttcttgaac
     2641 gtactccttg attttcaagg ccgcccgata ctctttcgga aaacgctgct tcactgaagc
     2701 cagcaaaaaa tcatccttct ccggcaactc ttccttctta atcattcggg caataaaaaa
     2761 ctgtaaatgc gtcacaaatc ttaagtaaag catcgaatct ttatcaacct ttttttcggg
     2821 aaacagatat cctagcatag ccaagatatc ctttagtgct cttaccgaga gcatggtttc
     2881 ttccatattt gcttgttcac tttgggcatt tacaatgtga aacgcaatgt ttccagcctc
     2941 ctcttctggc aactgaacat tcattttttt gttgatcaat tggatagcat agcagccgat
     3001 ctcaaattct tttgggtaat acttttgaat ttcaatcagc aggcgatttt gaaaaacaat
     3061 gcctttttga ctgcgttcaa tggcatgctt caagtgatca accaaggtaa aaaatagact
     3121 tttattcagc ttaaagtcaa acttggcgtt ggcataatcg ataattcgct tgacgcaaat
     3181 aattagatca tccggagcag cctcaaacag tttaatgtaa tcctttttta gagcatcaga
     3241 aagtgaatct aatttaaata ctttttggat tttgtcagcc ggaaccaaat cgcctttctt
     3301 tagttgaaat ccaatccctt tccccatcaa aattacttct ttgccttgct tatctaagca
     3361 aagaattaaa ctgttgttta acgttttaat tactctcatt agattcaccg ttctctctaa
     3421 gctaagccat ttcttgcgat tactttttga taccaaaaga agctttcttt ttttattcgt
     3481 tttaattctt taagattctc atcggttcgg tcaacatata taaaaccata acgcttattg
     3541 aagccttgat gggtactaac aacatcaatc gctgaccaag gtaaataacc aaaaacatct
     3601 acaccatctg ttaaagctgc ttgaatagcc agaatatgtt tggctagata atcaattcga
     3661 tcctggtcat taattcgtct atcttcctca actatgtcgg gtgccccata gccattttca
     3721 gtaattaaaa taggtaaatc atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca
     3781 gtagggtcaa ctacccagcc atatggcgtt ttatctacat aaggattttc agctgcccgg
     3841 tagacacctt cttctcccaa catgatctgt tgatcccccg ctctagcgga aacatctgat
     3901 ccgtcatttt tgctggcagc gatggtggca gttgaataat aattaatcgc aataaaatcg
     3961 ggtttaccgg aggataaaat tgcctgatcc tctgcctcaa ttttaggata taatccacga
     4021 tcattcaaat agttttcaaa caaataattg tattttcccc gaactgctac atctaagaaa
     4081 gaccagcctc tcaatgtttc ccaattatgg gctgcaattg catcttctgg actggaggta
     4141 gcttgataca tagccgttaa attgattgct ggaccgattt ttcctttaag attcaactga
     4201 tgatacaagt gaattactct agcttgagct aataaaacat ggtgattaat ttgaaagagt
     4261 tctttttttg aaggcagctc tccacttggt ggcaaaccaa tcgctccagg atgaaggatc
     4321 atcgtgtttt gctcattaat tgtcagccaa tagttcacct tgtcaccata ctcagtaagc
     4381 aatacctgta catagtccac aaaataatcg atagttgctc gatttaacca accgccctgc
     4441 tcctctagaa aataaggtaa atcgaaatgg tacatggtga caatcggttc gattccctga
     4501 ttcaggcatt catcaattac ttgatgataa aaagaaattc ccttttgatt ggttttatta
     4561 ttttcaatat ccataattcg cgtccaagcg atggaaaagc gaaaagcttt taagcctaat
     4621 tctttaaaca gctgtatatc ttctttatag tgatggtaaa aatccgaggc aacttcaaaa
     4681 ttagctaccc cttctgtatg ctgatacttg tcaataatag acggttgttt cccatcctcc
     4741 tgataggcac cctctgcctg gtaggcacta atggaggcac cccataaaaa cttctctgga
     4801 aatgctgaaa gctgtttgta ttgcacaaaa atcctctcct taactaattt gcatcaatgg
     4861 ttcgtgttcc tttaagcttc cgcttgcttc actaattcct gcgtaatttt cactgttggt
     4921 cactaccaca atcactgtat catcgtagcc agcgtttcta atggcagcga aatcggcgtt
     4981 gattaataat tgtcctgctg acacctgatc tcctgctttt acaaatactt caaagggttc
     5041 cccttttaaa ttcacggtgt cgatccccac atggatcaat aattcaatac cttctttaga
     5101 acgtagacca atcgcatgtt tagattctaa aatagtaatg acttctccct caacaggtga
     5161 ataaatgctg ttttcttctg gatgaatggc tacgcccttt cccaatagtc ccttagaaaa
     5221 gacagcatcg ttcacttcag aaataggaca cacggttcct tttaccggat tttcaacaat
     5281 gactgcttcg gctattgccg tttgctctga ttcatcagaa gtatcgtcaa atccaattag
     5341 ataagcaatg ataaaggttg caacaaaggc gattacacac gtaataattg cgtgaacaat
     5401 attcattgga ttttctccaa taaaggctgg taaggctgct aagccagggg aaacaaaggc
     5461 atatctgact aaaccgctca agccagcata caaaccagca attgcaccac caatcatgga
     5521 agcaactaat ggttttttca gcttcaaggt tactccatag agtgaaggct cagtaattcc
     5581 cattaaagca gtgatcccag tggacacagc tagctgcttc aactctttgt ttttagtttt
     5641 cgcggctaca gcaaaggtag cacctgattg agcaatatta gaagccagca ttcctggccc
     5701 attgatcatt tcagaacccg tactgctcaa ttgcatagta gcgattggcg tcattgccca
     5761 agctgtaccc gtaaccacca aaaatggctg caaaccaccc atcaaaaatg gaattaacca
     5821 agctgctttg ccgttaatcc attcagcccc cattgctata agatcgttca gcaacgttcc
     5881 aaacggtcca atgacaacca acgctaaagg ccccataatt aaaaatacca gcatcggctt
     5941 agtaaagaat ttaataaacg aaggcgaaat tcgatccgca aagggctcta cataggacat
     6001 gatccaaaca gtaaagataa tcggtaaaac tgatcccgca taatctgcag ctaataccgg
     6061 aattcctaga aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat ttggatgaag
     6121 caaaatgcca gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta tggctgcccc
     6181 ataggctaat agtactggca taaaataaaa ggctgcatca gcaatagtat tcaaaatgat
     6241 gtaagtttga ctactgtctg atagtacacc agtcaaagtc aagacggcca aaatggcttt
     6301 taccatcccg gcacctgtta tagctggaat catcggtgta aaggtggtag aaatgacact
     6361 aatcaatctt gaaataatac tttcttgatt gtctgaagag gggccctttt taattgaagt
     6421 tcccacttcc ttttgaattt ccttgaaggc tgtttggact tcattgccaa tcaccacttg
     6481 gtattgtcca ccgctatcca ccacactaat tacaccaagt gttttttcta aggctttcgt
     6541 atcggctttc cttttatctt tgagttcaaa acgcagtctg gtggcacaat gggttaagct
     6601 gaccacattt tcttttcccc caacctgttg caatacattt cttcctactt ctctgtaatc
     6661 cattattctc tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca
     6721 ttgaagtgtt agtttgtctt ggtcttgcct gcataatcag taacatgcca ataacgttat
     6781 tttgctgtaa tccaataata gcacattatg atagcgcaat caacagggaa tttaattttt
     6841 tatcttttcg tttgaccgta tagggtaata taacagttag atcacatcaa ctagggtata
     6901 tctgcgtata cttactgcta aaataagtat atctaatatt aactatacca gtaaaaccat
     6961 tcctacataa ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg catataacgg
     7021 atcacacaac ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt gagtaaagga
     7081 tggaataaac agcattccaa caatataggc aacaatagtg atacttgttt ttttggtaaa
     7141 aatcagtaag caatagccaa tttcaatcat tccgagatat aaaggtatcg atagaatgat
     7201 ttgggctagt attcttaaaa attaactagc aaatgtttaa actgatttat ttctaatcta
     7261 catgtagtaa gtatttcaat tccaccaatt atcaataaaa ataccaaaca tatccataat
     7321 aattttttct ttttcatttc ttttatattg ttataacagt gaataacata aaaattcccg
     7381 acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag gaaactataa
     7441 aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg cataatacat
     7501 ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat acggtctttc
     7561 tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat agctttagcc
     7621 cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga tcgatttaag
     7681 ttgtactgtt cttgtaaaat ctgtgttacc aatggtttcg atgacttctc taatccagat
     7741 gtacctgtca tttttgaaaa aatgatacct ataatagcca agacaacgat aactataatg
     7801 ataaacttct tgttcatatt tactttattc aactttttat ctcctatctc caaaataatt
     7861 tgctcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa gattgctcca
     7921 gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttatctcg atataagtag
     7981 cccataataa cagcaacagc accaaataca acaggaataa atgctaacgc tactaccata
     8041 gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc
     8101 ttcatttcat taattgtctg ctcagtactt ttctttactg cgtctgctcc agttgtctca
     8161 tctgtttctt gtagtgctcc gcaaaactgg caatacttac tttcatctgt tatttctttg
     8221 ccacattttc tacaatacat gtcttctcct cctaaaaaca ttcaattgtt tattagttaa
     8281 ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact taatatctca
     8341 gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca
     8401 cattgctgtg taacattcat ttttttgcta actccgtttg agaaagtcct ttttcttttc
     8461 tgtatcggct gagattactt acttttttca tgattctccc ctcctgtttt attatataca
     8521 acaatatgat gtatttcaac tcataaaata taaaaaaact tcaaatacca ttacaaatac
     8581 aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa caatatgcaa
     8641 aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa
     8701 tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag cctaacctcg
     8761 atagcttaac cgctctttct agagccctag gaacccctgt aagccatttt ctagacaatg
     8821 ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc actgaagatg
     8881 cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc
     8941 tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga
     9001 aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc ttttctcttc
     9061 cttactctcc gtccatcatc tcttgttaca cttgtaaatt gataaagagg aactaattca
     9121 actcctgata atatcaattc ttttattact tcatctatcc ctgctccttt tgctaggtag
     9181 actaaaaaac tactacttgc tttcacattg tatagtctct taatatcctt agaagctcgt
     9241 tcaagagtga atccttgttt aacaaagtag cctctgtttt cttttaaagc attttccacg
     9301 ctgttattcc caacattacg tagacgttca caaacagtgt tagtaagttc tttctctgtc
     9361 attagatatg ttccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc
     9421 aattgttttt ccttgctaag ttctgatcgg gaattttgag gaaatactct tttagcctct
     9481 ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaaat
     9541 ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa
     9601 acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc tttctcttcc
     9661 tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt
     9721 atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt
     9781 ttaccatagt aaaaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca
     9841 ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc
     9901 atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg
     9961 tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg
    10021 ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca
    10081 actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc
    10141 ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt
    10201 aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc
    10261 tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct
    10321 tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag ttcatactct
    10381 ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca
    10441 gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca
    10501 aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag
    10561 tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca
    10621 atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa
    10681 ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact atcatcttta
    10741 ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt
    10801 actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata
    10861 aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta
    10921 tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt ctctaataat
    10981 ttttctttat ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc
    11041 ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta
    11101 tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc
    11161 agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac
    11221 atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat
    11281 atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat
    11341 ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat
    11401 atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt
    11461 tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt
    11521 tcatcgacat acacataata cctcgataaa aaagaggtgt caatatcttt gtaaaattta
    11581 tttacaacac ccctttttcg aggtatacta attttaggag gtgtggtcaa tatgataaaa
    11641 cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata
    11701 agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta
    11761 attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga
    11821 ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta
    11881 gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca
    11941 tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat
    12001 ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct
    12061 aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata
    12121 gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact
    12181 agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa
    12241 gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa
    12301 caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc
    12361 atctagtggt aagtactcct tgtaaactaa gaggagatga tttcatggca aaaatatcaa
    12421 agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc
    12481 cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc
    12541 aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg
    12601 atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag
    12661 cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta
    12721 agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca
    12781 gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg
    12841 ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag
    12901 agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt
    12961 ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta
    13021 tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac
    13081 tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga
    13141 aaacaagagc ttctagacga gttatctctt tagatactga gactctttct atcctcaaca
    13201 actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag
    13261 agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt
    13321 ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac
    13381 atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat
    13441 tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac
    13501 ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa
    13561 aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta tatat
//
LOCUS       DAZKOW010000079        13429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000079 DAZKOW010000000
VERSION     DAZKOW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.079"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..914)
                     /locus_tag="ACG912_002141"
     CDS             complement(<1..914)
                     /locus_tag="ACG912_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFQ0513438.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="ACG912_002142"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="ACG912_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFQ0513439.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="ACG912_002143"
     CDS             2006..2176
                     /locus_tag="ACG912_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513440.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="ACG912_002144"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="ACG912_002144"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFQ0513441.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="ACG912_002145"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="ACG912_002145"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFQ0513442.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /gene="dapA"
                     /locus_tag="ACG912_002146"
     CDS             4440..5321
                     /gene="dapA"
                     /locus_tag="ACG912_002146"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFQ0513443.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /gene="dapB"
                     /locus_tag="ACG912_002147"
     CDS             5318..6034
                     /gene="dapB"
                     /locus_tag="ACG912_002147"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFQ0513444.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="ACG912_002148"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="ACG912_002148"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFQ0513445.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="ACG912_002149"
     CDS             6751..7752
                     /locus_tag="ACG912_002149"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFQ0513446.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="ACG912_002150"
     CDS             7749..9110
                     /locus_tag="ACG912_002150"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFQ0513447.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="ACG912_002151"
     CDS             9070..10221
                     /locus_tag="ACG912_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFQ0513448.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="ACG912_002152"
     CDS             10196..11365
                     /locus_tag="ACG912_002152"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0513449.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="ACG912_002153"
     CDS             11540..12919
                     /locus_tag="ACG912_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0513450.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="ACG912_002154"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="ACG912_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4484 a         2339 c         2924 g         3682 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAZKOW010000080        13212 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000080 DAZKOW010000000
VERSION     DAZKOW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.080"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            147..1016
                     /locus_tag="ACG912_002155"
     CDS             147..1016
                     /locus_tag="ACG912_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0513451.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="ACG912_002156"
     CDS             1225..2250
                     /locus_tag="ACG912_002156"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFQ0513452.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKTVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="ACG912_002157"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="ACG912_002157"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFQ0513453.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="ACG912_002158"
     CDS             3333..5036
                     /locus_tag="ACG912_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0513454.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="ACG912_002159"
     CDS             5038..7071
                     /locus_tag="ACG912_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFQ0513455.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="ACG912_002160"
     CDS             7082..7522
                     /locus_tag="ACG912_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0513456.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="ACG912_002161"
     CDS             complement(7722..8849)
                     /locus_tag="ACG912_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513457.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="ACG912_002162"
     CDS             complement(8852..9529)
                     /locus_tag="ACG912_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFQ0513458.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="ACG912_002163"
     CDS             9793..11559
                     /locus_tag="ACG912_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFQ0513459.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="ACG912_002164"
     CDS             11574..12011
                     /locus_tag="ACG912_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0513460.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGGVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="ACG912_002165"
     CDS             12024..13175
                     /locus_tag="ACG912_002165"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFQ0513461.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2731 g         3699 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aaacggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgtttg catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggggcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAZKOW010000081        13153 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000081 DAZKOW010000000
VERSION     DAZKOW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.081"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            117..1856
                     /gene="yfcC"
                     /locus_tag="ACG912_002166"
     CDS             117..1856
                     /gene="yfcC"
                     /locus_tag="ACG912_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFQ0513462.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2013..3197
                     /gene="iadA"
                     /locus_tag="ACG912_002167"
     CDS             2013..3197
                     /gene="iadA"
                     /locus_tag="ACG912_002167"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFQ0513463.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3308..3511
                     /locus_tag="ACG912_002168"
     CDS             3308..3511
                     /locus_tag="ACG912_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFQ0513464.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3612..4274)
                     /locus_tag="ACG912_002169"
     CDS             complement(3612..4274)
                     /locus_tag="ACG912_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513465.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4287..5789)
                     /locus_tag="ACG912_002170"
     CDS             complement(4287..5789)
                     /locus_tag="ACG912_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513466.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESRETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6337..8016
                     /locus_tag="ACG912_002171"
     CDS             6337..8016
                     /locus_tag="ACG912_002171"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFQ0513467.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8143..8622
                     /locus_tag="ACG912_002172"
     CDS             8143..8622
                     /locus_tag="ACG912_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFQ0513468.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8841..9521
                     /locus_tag="ACG912_002173"
     CDS             8841..9521
                     /locus_tag="ACG912_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFQ0513469.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9529..10860
                     /locus_tag="ACG912_002174"
     CDS             9529..10860
                     /locus_tag="ACG912_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFQ0513470.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10875..11837
                     /locus_tag="ACG912_002175"
     CDS             10875..11837
                     /locus_tag="ACG912_002175"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFQ0513471.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11948..12286
                     /locus_tag="ACG912_002176"
     CDS             11948..12286
                     /locus_tag="ACG912_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFQ0513472.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12365..13072
                     /locus_tag="ACG912_002177"
     CDS             12365..13072
                     /locus_tag="ACG912_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFQ0513473.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4180 a         2321 c         2883 g         3769 t
ORIGIN      
        1 aaaagtgatt ttctaataaa ttattcatgt ggaaatgtca tttttactgt ataattcatt
       61 tgattatcca ttttttattc atcaaaatta ctgaaaaaag aaaggaagaa ataaatttga
      121 agaaaacaaa ttcttcagga aaaccgaaac gcgacttcac taaattatcg acaccccata
      181 cgtatgtcat tatttttggt gtagtcatct ttgcatggat actgactttt gtcgttccag
      241 caggaaagtt cagcacgcaa gatattgaat acaaagatgc aaatggcgaa acaagtactc
      301 gtactgtttt acgtcaagat tccttccgtt atgcttacga gctggataag tcgtatgtgt
      361 ttgatcaatt agaagaactg caagatcatc cagcagaacg tgaaaaactg gatgttccgg
      421 aaaaaggatt ggaaaaagtc attgctgacg gagaaaaaaa tcttacgcag gaaaaactag
      481 atgaaatctc cttgacagat gatgtgcttt acgatcagta cggcgaaaat atctatgata
      541 cttctaaaaa attgcataaa acagccaaaa tatggggaac agatgatttt ggcggctttg
      601 gtttcttaaa ttttgttttt gaaggactag tatctggaga taagtatgga tctgctgtag
      661 ggatcgctgc attgatcctt gttgttggag gtgcttttgg tattattatg cgtaccggag
      721 cgatcgacgc aggtatctat gcgtttatca gtaaaactaa gggactggag cgtttagctt
      781 taccgctatt attctttgct ttttcattcg gtggcgctac attcgggatg gcagaagagg
      841 taattccatt ctcaatggtg atggttccat ttgtcattgc gttaggatat gactcgatcg
      901 tagcagtaac cgtgacttat gtcgcatctc aagtcggaaa tgcgacctct tggatgagcc
      961 cgtttagcgt agcggtggct caagggattg ctggaatccc cgtgttgtct ggtgcgacat
     1021 tccgattgat tatgtgggta gttgttactg cattagccgc tggttatatg atgatatatg
     1081 cagaaaaaat ccgtaaaaaa ccagaaagtt cgttgactta caaatcagat gattatttcc
     1141 gttctcatat caaaaagaca tctgatgaaa ataaaaaatt catgcttggg cataaattga
     1201 tcttattaga gatgttagtt gtccttgtct ggatcgtttg gggcgtgact caaaaaggct
     1261 attacattcc agaaatcgct tctcaattct ttgtgatggg attagttgct ggaatcattg
     1321 ctgttctttt caaattagat ggaatgaaag tcaatgacat cgcttcttct ttccaatctg
     1381 gtgcagctga tttagctggt acagcaatcg tagtaggtat ggccaaaggg attcttcttg
     1441 tcttgggagg atctgatgca tctgttcctt cagctttgaa tacgattttg catgggatcg
     1501 ggacggcgtt gaccggtgtt ccagctgtca ttggtgcttg ggcgatgtac atcttccaaa
     1561 gtttgttcaa cttggttgtt acatcaaatt caggacaagc agctttgact atgccaatca
     1621 tggctccact ggcagattta gtaggtgtat ccagacaagt agcagttctt gcttatcagc
     1681 ttggttcagg ttttatggac gcttttacac ccgtatcagc cagcttgatt ggtgtgctag
     1741 gtgttgcacg gatcgaatgg gcaaaatggg caagattcca aatcaagatg caaggattcc
     1801 tgtttgtttt aggaactatt ttcatcatga ttgccattgc aataggtctg cagtagtact
     1861 ggagacctgt aaataaaaaa gtaatccaaa cagtagataa ggactccaag aaccgtcatt
     1921 ttacggtaaa atcaaaggag taaagatcct atcgtttgga ttatttttta agatcctatc
     1981 atcagttata cttaaagaaa gaggaaaaat gtatgaaaat cattcgacaa attgaagtgt
     2041 ttgcaccaga ttacttagga aaaatggatg tactgattgc cggtgggaag attttggctg
     2101 tagaagaaca gctagaaggc ggctacgaag gagtggaagt agaagaactt tcaggtgagg
     2161 gaaaagtact aaccccgggt ttcattgact gtcatttcca tattttaggt ggaggaggag
     2221 aaggcggtta tcaaaatcga acgccagaag tcacattgag ccaattgact acagctggtg
     2281 taacgacagt tgtcggttgt ttgggaacag acggagaagg tagagatatg actgcgttga
     2341 tcagcaaagc gaaaggtctg gaagcagaag gaatctccac ttatgtatac gaaggttcgt
     2401 accgattgcc ggtaaaaaca gtaacagatt ctatcatcaa agactttttg acaattgata
     2461 aaattatcgg aatcggagaa atcgcagtat ccgatcaccg ttcttctcag ccaagttttg
     2521 aagagttcgc tcgtgcagta gcagatgctc gtgtaggagg catgttatcc ggaaaagctg
     2581 ggatcatcaa tgtccactta ggcggaggaa aaagaaagct agagctattg acacgaatcg
     2641 tagaagaaac agaaattccg atcacacaat ttttgcctac tcatgcaaat cgcacaccag
     2701 aattatttga agcttgcgtg gcatttgcca aacgtggtgg cacgattgac ttcactgcta
     2761 gcgaagatcc tgatttttgg gagaagacag atggtgaagt tcgcttcagc aaagcactga
     2821 aacgattgat cgaggaagat gtttctttag ataatttcac gatgacctca gatggacaag
     2881 gaagcttgcc ttattttgat gaaaacaatc atttcctagg gttaggagta ggaagtgcca
     2941 aggcattgct agtagggatc aaagaagctg tgcaaaaaga aagtatccct ttggaaatcg
     3001 ccttacgagc tataaccagt aatccagcac gtatattgaa attggataaa aaaggaaaaa
     3061 tcgaaattgg tgctgatgca gatctgtgta tcttggataa agaaacgctt gatattgata
     3121 cagttatcgc taaaggagaa ataatggtcc aagaaaaaga agtcaaagta tgggggactt
     3181 tcgaaaaaag tttttaatca acagctctaa ctatcttttt tagaaaacga gcgttctgtt
     3241 tgtctgtatc tgttgttgat gttaccctga aggtagatca ataaacaaac taagaggagg
     3301 aagcacaatg gcagatttaa aaggacgttc taaagatatg aaagataaag tagtcggtga
     3361 aggaaaagaa gcatacggca aagcaacaga cgacaagagc aaagaaacag aagggaaagc
     3421 acaatcacta ggtgctgaca tcaaagaaaa agctcgaaat ttaggcgacg aagtcaaaga
     3481 caagttttct cgaaaagacg aaaaaaaata aaaaccactt attactaatt gaatcaagca
     3541 aataaaaaat ccgaaccata tctgcgagac agatatggtt cggatttttt atttgatcat
     3601 tatcctgttt tttaaacttc gattttttga aattttttat tttttagttc aaagacttca
     3661 tcacagcttt ttgctacttc aatctcatga gtcacgatga taatacagcg attttgttca
     3721 tgggcgattt cttggaaaag tttgacgata tcttgtgaag ttgtttcatc tagatttcca
     3781 gtaggctcat ccgctacgat tagttgatgt tcacagcaga tagcacgaac gatagcgact
     3841 cgctgctgct gacctcccga aagtttggtc acctgttttt ttgctaatat ttcatcaatt
     3901 cctacacgag ccagtcgatc taaagcaact tgtcgataat tctcttgctt ggtcttagtg
     3961 atttccattg ctgctatgac gttgtctaga gccgataaat aaggaagcaa gttgtaagct
     4021 tggaaaataa tcgagacatc atttttacga taacttctta acccgatttt gttcagtgaa
     4081 cgatcttgat agtatacctt tccttctttt ggtgcatcta gtccagaaat aagcgaaaga
     4141 aaagtcgttt ttccagaacc acttgctcct aaaatagcgt acatttttcc agcttcgaaa
     4201 ttcagttcta catctttata aagcgcatct tgcggatctt gataccaaaa tcccaattgt
     4261 tctgtccgta acatcaaatt cctcctttag tttaatagga tttttcttgg gtttaatctt
     4321 aatatcccta tagaagctaa aaagacagaa aacagactga ttccaaatgc tactccggct
     4381 aatagtccca attgtgcagg tttgacattg atttccaatt cgctgacttg atcagaaaca
     4441 gcaaatggat tattcgtggt tccttgttga ccatcgcgtt gaccgccttg ttcttgtctg
     4501 ttacctggca tttgtccacc gccaggagct tgctggtttt gtgtcgtggt ttctgtctgt
     4561 tggtcaagta gctgctgtcc cacggcattc gcgacgacgt ttccgctaag cgaagcaact
     4621 cctaatgcaa gaatcataca aatagccact tcggtaaaca attggagaat gattttactt
     4681 ctggattctc ctaaagatag caagacgcct aactcatgcc gacgctcccg aatggtaatc
     4741 ataatgatca gagtaagtat gataataccg gctacagcca ctagtaaaac aacattttta
     4801 gaaaaacttg ccacgttgtt taaaggttcc aacattgact gatacatgga atcatttgat
     4861 tgtaagctaa atgtatctgt gtcgatcagc ttctcagctt ctttgagaaa ctcatccatt
     4921 ttatctggat cagatagagt gtaaatagca gaatctatcg tgttatcgtc agatgtccca
     4981 tttagttcat ttgcaaaagt gtaagaagtg taaagtgtat tcgacggatt catgaagttg
     5041 aatttcatac ccatactgcc gcttgtttct ctggattcat aaattccttt gatcgtcatt
     5101 tcataagtgg tatcttcatc ttcagtactc gtgattgtga aagtatctcc gactgccagg
     5161 tcatttgttt ctgctagagt agaatcaatg actacattgt ttgtgccttt gtcgctgtca
     5221 tctattcctt caccatccgt gatttttgct gttccatcag aaaaattact attttgtgca
     5281 gttgctgaca caccactgat ttgaaagtct gcttgagtca tttgcggatt cccgcccatt
     5341 cctccagcag gttcaccgcc tgcatcagta gtttctgaac tgtcttcact tgaatcagaa
     5401 ctagtgatgg cagttattcc atcttttgcc atagctgagg cggaactttc aaaggaataa
     5461 ctctttacat tatctaattc agctattttt tccgcgtccg ttaaggaaac tggtgtaaga
     5521 gaaaaactgc ctggatctgg acgttcttca gtggtagaac cgcttgaaga agtttgttcc
     5581 tgttttttaa atgaagcttc tcgattggca cttaaagtca ctgttgctcc aacttctttt
     5641 tgtgcttgat tcgctgcttc ttctgcagcg cttcgaatcg tgaggccagc tagcacaaaa
     5701 ataagaatgg ctgtgaacac ggcaaacaat aacaatgaac gtccccattt tacttttgta
     5761 cttttccatg cacgtttcat aaaattcata ttttgcatcc ctcctagtag atgcatttga
     5821 taatagacaa cctatcttaa agctagctta aatcttatgc tgtttgcaca ccatcaacat
     5881 gaggttctgt atatataaaa agtcactata gttattgcaa aaaatgtggt aaaatgaaaa
     5941 aagtatgttt caaaccgacg aagaagaaat gaaagaggaa tagcaaaaga aatcctagat
     6001 gctacagagg gatttttacg tgcatagttg ttctaacaat tgctgatact agcgatttag
     6061 tttatttgtt gtataataat ggggaacagc tgaatactta tataagtatt ctggctatag
     6121 cgtttcgcaa aagatccatt ttgttaatga agtatatcca aaataaaatg cccatccaac
     6181 tgtcagaacg cggatgtgct ggtaattttc ctgctgattc tccataagtt gagaaatagt
     6241 gattttgcat atgcttcatt ggaatggctc atatatttag ataaagaaga aaatcgacat
     6301 ctttaaactt ggatgtcata agaagaggtg aagtttgtga gtaccattaa aattatccca
     6361 ttaggcggcg ttcgtgaaaa tggaaaaaac atgtatgtcg cagaagtggg cgaggatatt
     6421 tttattttag attgtgggct aaaatatcct gaaaatgagt tgttggggat cgacgtcgta
     6481 atccctgatt ttacgtattt agaagaaaac attgatcgtg tagctggtgt ctttttaaca
     6541 catggacatg cagatgcgat cggggcactt ccttacttgt tgtcaaaaat ttctgtacca
     6601 gtattcggga cagagttgac gatcgagtta gcaaaattga atgtcagtcg taatgaagca
     6661 gccaaaggat ttaaggattt ccatgtggta gatgagcata cagaaataga ctttggtgaa
     6721 acagtagtga gctttttccg taccactcat acgataccag attcagtggg gatcaatctg
     6781 aaaacaaaag aaggtagtat cgtttatacg ggagatttta aatttgacca aacggctatc
     6841 ccaatgtatc aaacagatta tgcacgattg gcggaaatcg gcaaagaagg agtcttagcc
     6901 ttactaagtg aatcggcaaa tgcagaaaat cctcagcctg tagcttctga agcccaaata
     6961 gcagatgaag tatttgatac gatcaaatat tgggaagggc gtatcatcgt cgcttgtgta
     7021 gcaagtaatt tgcaacgagt gcaacaagta ttggatgcag cagaaaaagc acaacgaaaa
     7081 gttgtcttga ctggtcaaga tttcgaacga attattcgca cagcgatgcg tttagaaaaa
     7141 cttcaattac caagtgaaga tctgcttgtt acgttgaaag acatgaaaaa ctatgatcca
     7201 gaagaactga tcattttaga aactggccga atgggtgaac ccatcaagtc tctgcaaaaa
     7261 atggcaaacg gtacacaccg taatgtcaag attgaagaag gagatttggt ttatatcact
     7321 actacgccaa gcatcgcgat ggaaaccatc gtggcgaaaa cggaagatat catttatcga
     7381 gctggtggaa cagtgaagtt gatctctgag aatatgcgcg tttctggcca tgccaatcct
     7441 aatgacttgc aattaatgat caatttgatg aaaccaacat attttgtgcc agttcaagga
     7501 gagtatcgtg aattagctgc tcatgcagat ttggctcatg cagtagggat gccatacaaa
     7561 aatatctata ttactggacg cggagacgtg ctggaatata agaacaagcg tatgagtgtt
     7621 gcaggaacta caagtgcaga aaatgtaatg attgatggat taggcatagg tgatatcgga
     7681 aacattgttt tacgtgatcg aaaaatcttg tcagaagacg ggattttcgt agcggttgtc
     7741 actatcagtc ggcgagaaaa gaaaatcatt tcaaaacctc aaattacttc acggggattc
     7801 gtttacgtaa aagctagccg tgatctgatg aaagaaagca gcaatatgat tgaaacaatc
     7861 gttgagaaac atttagaaag caatgatttt gagtggagca aattaaagca agacatccga
     7921 gatcaattag gccgttactt atttgaacaa acaaaacgcc gcccagtgat cttgccagtc
     7981 attatggaag ctactcaacg tcgaaataaa aaataaacaa aaagagtaag atgaacctct
     8041 ctatagagtg ctcgtcttgc tctttttttg cgaaaaacaa ataatttttg aatacagcat
     8101 ccctcgcttt ataataaacg atagaataaa gggagtgaat caatgatgag tcaaacaaaa
     8161 gtcgaacgtt atttagaaga acaaaagatc agctatcaac cacttgattt tagtcatgtg
     8221 gtagaagaat ctttcacaga agaactacaa aaaagaggga ttgatccagc tttgatatgt
     8281 aaaactctcg tagtgaaagg aaacaaaact ggggtgatca tcgcattgat tcctttgaac
     8341 gatcacttag attacaaaaa aacgagacaa gtgacgaaag atcgtaaagt tggttttcct
     8401 ggaatggatt ttgtattaat gcatacagga tatccccacg gagcaaacac ccctattgga
     8461 ataaaactcg cacatcctga ttattgtttt ttagcagaca gttctttaaa agaaaaagaa
     8521 gaagtaattg tgtcaagcgg tgaaatcggg agaagccttc ggataaaagt agccgattta
     8581 gaaaagatcg tccatcctat ttacgctgat ttaattagtt aagggacgag aagagaagct
     8641 aagcagagca gagacctcaa acttgagatt atatctgctt ggtacttggt gcatcctgta
     8701 ttatctgtta caattgcttt gtacatactg tactatcaat ccaatgaaaa tatatttagc
     8761 agctatattt tttcattgat gtgcaagtga tgaaatcgtt tttaataaaa aagatacaga
     8821 gaaaacgcga ggtgtaaaat atgaaaatta cgtatcatgg acattcctgt ttaacaattg
     8881 aaatggaaaa tggccaacgc gtgatgattg acccgtttat tacaggaaat ccattgacag
     8941 acttaactgc agaagaagta aagacagatt ggttgctgat tactcatggt catagtgacc
     9001 acataggaga tatgattcct attgcaaaag aaaatcaagc cacaattatc agtattgttg
     9061 agattgctga ttatgcgaac agcagaggag tagattcatt cggtatgaat atcggtggaa
     9121 aacatgcgtt tccattcggc gtggtaaaat tcgtacatgc acagcatagt tcaagctatg
     9181 aagtagacgg tatcgttcag tatatgggcg agcctagcgg aattattatt caagcagaag
     9241 gaaaaacgat ttatcatgca ggggatactg cttatttcag tgatctagga ttgcttgcag
     9301 aagaattcga tattgatgtc gctttcttgc cgattggcga taactatacg atggggccag
     9361 aagatgcagc aaaggctgcg caaacaattc gtgccaagaa agtagttcca atccattaca
     9421 atactttccc tgtcattgaa caagatcccg aatcttttgt agagttactg gaagatagtg
     9481 aaggaaaaat aatgagcgtc tcggaaacgc tggaagtgta ggcagaaaat ggcgacaaaa
     9541 catgatttga ttttagaata tattgaaagt ttacctgttg gcaatcgaat ttctgtaaga
     9601 agcatcgcaa aagaattgaa tgtcagtgaa ggaacagcgt accgtgcaat caaagatgca
     9661 gaaaatgtcg gactagtttc aacaattcaa cgagtaggga ccatccgtat cgaacgcaaa
     9721 ttaaaaaaac atattgaacg tttgacattt ggcgaggtcg tcaggatcat tgaaggggac
     9781 gttttaggcg gggcggcagg attagacaag gtcttgaata aattcgtcat cggcgcgatg
     9841 actaaacatg ccatggaacg ctatattacg ccaggatcac taatgatcgt cggcaaccgg
     9901 gcggaagtcc aaaaattagc attggaagat ggcgccgcag tattgatcac aggaggattc
     9961 aatacgactg ctgagattgc aaagctagct gatgatcttg aaatgccgat cttacgcacc
    10021 tcttatgata catttacagt cgcaaccatg atcaaccgag cgttaagcga ccaattgatc
    10081 aaaaaagata tcatgcttgt cagtgatatc tatatgccag ctgaaaagac tcattatttg
    10141 cagcagacag atacgatttc tgattatcag cgtttgtcag aagaaacgca gcattcgcgt
    10201 tttccagtcg tcaaccgcca tcatcgtttg atggggattg ttacggcaaa agatgtttta
    10261 gggaaatcgc caaaccagat catcgatcgg gtgatgacga aagaaccaat cagtgtaaag
    10321 aaaacaatga gtattgcctc tgttagccat caaatgatct gggatgggct agaagtcatg
    10381 ccggtcgttt ccgatgactt gacattggaa ggcttgatta cccggcaaga tgtaatgaaa
    10441 gcgatgcagc ttgtccagcg acaaccgcag attgcagata cgatttcgga tcagatatct
    10501 ggaaatatcc atacgattga tactgaccgt gaaggaaatc gtttggaaaa ccctaagttc
    10561 aaattcgtag tagcaccaca aatggtcaat ggcgttggaa ccatttcttt tggcgtactt
    10621 agcgaaatca tttctgatgt cgcacaaaaa acaatggtga tgaaccaaaa acgaaatatc
    10681 ttgattgaac aagtgaacct acattatttg cggctgatcc aattggaaag cgaattagac
    10741 attcgtccac gtgtattgga aatcggcaga cgttccgcta aactggatat cgaagtgttt
    10801 attgaaaaca ccatcgtttc caaagcaatc gttgtttgtc aagtaatgga acgttcttag
    10861 aaagcaggag tgaaatgaca gtacagcaag aaatagtaga tacaataaaa aaatacgata
    10921 aaatcattat ccacaggcat ttgcgccctg atcctgatgc attaggatca caagtaggat
    10981 tagccgaact attacgggaa agtttcccag aaaaagaaat acgtcaagta ggtgaaacga
    11041 ttgaagggct tcgcttttta gcagaaatgc aagaagttga agatgtcttc taccaaggag
    11101 cgctagtcat cgtgactgat acagctaatt cacccagaat cagtgatcaa agatatcagt
    11161 tggcagaaaa actgatcaaa atcgaccatc atcccaatga tgacccttat ggagatcttg
    11221 tgtgggtaaa tacaaaagca agtagctgta gtgagatgat tgcagaattt gctttgatgt
    11281 ttcctgaatc tttaaagatg aacgttaagg ctgcacggtt actttatgca ggaatcgtcg
    11341 gagatacagg gcgctttttg tatccctcca cgacgtcccg tacattagag attgcttcga
    11401 tattgagaaa ttatccattt gatgcaagtg ctttgaacag agagattgaa caaatgccga
    11461 tgaaagtcgc taaactttcc ggttatcttt atcaaaatat ccaggtggat gaaaatggtg
    11521 caggaagaat aatccttccg caagaaattc ttgataaata tggaatcgat gattcagaga
    11581 cagcttcgat cgtttcactt cctggagtga ttgacgaagt gctggcttgg gggatttttg
    11641 ttcagcagcc ttcagggtat taccgcgtac gtttacgttc taaggggccg atcatcaatg
    11701 aattggctaa acgccatcat ggaggaggcc atccattagc aagtggtgcg aatgcaaaag
    11761 atttagcaga agtagaggaa atctataaag agatccaaga tatatgccaa tcgtataaga
    11821 gcagaaaaac agaatgatac ttcgttcgaa aacgtgcctt ttgtagtaaa aatcacaaat
    11881 gtatcttttc aagatcttct ttttccgtta caatagtgct cactaaagat tttaaaggag
    11941 aaagaagatg acattaccaa attgcccaga atgtggctca gagtacacat atgaagatcg
    12001 aggcttgttg atttgcccag aatgcgggaa cgaatggaac aaagaagaac aagaaactgt
    12061 catagaagac ggattagtgg tattagatgc aaatggaaac cgtctgttag agggcgatag
    12121 cgtgacagtg attaaagact tgaaagtaaa aggtgcgtca ggtgccatta aacaaggaac
    12181 aaaagtcaag aacatccgtt tagtagaagg cgatcataat atcgattgca aggtagaagg
    12241 atttggaccg atgaaactga aatcggaatt tgtgaaaaag aattaaagaa tattcaaggt
    12301 cttttcacat tcttttccta ctactaatga taaaataaag taaacgaaaa ggagtgaaac
    12361 gaaaatgtat ggttatggaa tgtatggttt tttagatccg acatttcttt tggtcattct
    12421 tggattagtt atttccggaa ttgcgtcagc gtacgtcaat agtactttcc gaaaatacga
    12481 tcaagtgaga agcaaaaaca atgtgactgg tacacaagca gcacaatata tattacaaag
    12541 tcaacagata aataatgtcg gtgttcaaca aattgccgga gatctcacag ataactacaa
    12601 ttcaggtaat aagatgttga gtttgtctca agcaactgca cagtctactt cagttgctgc
    12661 gataggtgta gcagctcatg aatgcggaca tgcggtacaa gatgcttcgg gttatgtccc
    12721 attgaagatt cgtgcagcac ttgttccagc agctaacttt ggttcgatga tttcgttccc
    12781 cttgattatg gtaggtgtat tgcttagctg gaactctacg ttaatcaata tcgggatttt
    12841 cgctttttct ttagcattac ttttccaatt ggtgacatta ccagtcgaat ttaacgcgtc
    12901 tcggcgagca atccaaattt tgagtgaagg cggactactg acggaagaag aagtaccgat
    12961 ggcaagacac gtattatttg cagcagcact gacttatgtt gcagcagcgc tttcgacatt
    13021 cctgcagctg ttgcgcttga tcttattgtt cggcggcaac cgaagagatt aaaataaaga
    13081 agttgtgaaa tcagcgcttt gatctgagct aataccgaga gttagctgat tgaataccat
    13141 ttatttggaa taa
//
LOCUS       DAZKOW010000082        12920 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000082 DAZKOW010000000
VERSION     DAZKOW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.082"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..730
                     /locus_tag="ACG912_002178"
     CDS             <1..730
                     /locus_tag="ACG912_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0513474.1"
                     /translation="SILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNV
                     ALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSY
                     GGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINK
                     GEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLL
                     GSIQTNLPESVLLDCGMDFLKDIC"
     gene            788..2827
                     /locus_tag="ACG912_002179"
     CDS             788..2827
                     /locus_tag="ACG912_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFQ0513475.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2936..4087)
                     /locus_tag="ACG912_002180"
     CDS             complement(2936..4087)
                     /locus_tag="ACG912_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFQ0513476.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4241..4771)
                     /locus_tag="ACG912_002181"
     CDS             complement(4241..4771)
                     /locus_tag="ACG912_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFQ0513477.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4870..5220)
                     /locus_tag="ACG912_002182"
     CDS             complement(4870..5220)
                     /locus_tag="ACG912_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFQ0513478.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG912_002183"
     CDS             complement(5377..6906)
                     /gene="ahpF"
                     /locus_tag="ACG912_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFQ0513479.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG912_002184"
     CDS             complement(6916..7479)
                     /gene="ahpC"
                     /locus_tag="ACG912_002184"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFQ0513480.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7623..8285)
                     /locus_tag="ACG912_002185"
     CDS             complement(7623..8285)
                     /locus_tag="ACG912_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0513481.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8469..10115
                     /locus_tag="ACG912_002186"
     CDS             8469..10115
                     /locus_tag="ACG912_002186"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0513482.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG912_002187"
     CDS             10106..11188
                     /gene="uxuA"
                     /locus_tag="ACG912_002187"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0513483.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11296..12849)
                     /locus_tag="ACG912_002188"
     CDS             complement(11296..12849)
                     /locus_tag="ACG912_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513484.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
BASE COUNT         4326 a         2368 c         2389 g         3837 t
ORIGIN      
        1 atctattttg ttactgctta cattagtagt tggctatttt tcgattgagt ttatccatgg
       61 attttcgtct gcaaaacaga gttcaactgt gaaaaaggta gagccgaaaa gtatccctac
      121 cacactaaat gtggctttga ttggttcaga tgctcgttcg aaagacgaaa acggtcgctc
      181 ggattcactt ctggttgcac aatacaacca gaaaacacaa caagcaaaac tgatctctat
      241 catgagagat tcatatgtcg atattccagg ttacggaatg aataagataa atgcagctta
      301 ctcttatggg ggagttgatt tattaaatca aacattaaag gaaaatttca aatttgaagc
      361 tccctattat gcaagtatca catttcaaga ttttatcgat tgcgttaatg aactgtttcc
      421 tggtggagtc aagatcgatg cagaaaaatc tctagattta gatggcgtat atataaacaa
      481 aggagagcaa gtgatggatg gtaatacttt actccagtat gctcgatttc gggaagacaa
      541 agaaggagac ttcgggagga tcagaaggca gcagcaagtg atcaaagcta tctctcagca
      601 gctgaaagac gtaacttcaa tattcaagct acccaaagcg gttgggaaat tacttggtag
      661 tatccaaacg aaccttccag aaagtgtgct tctggattgt ggtatggatt tcctcaaaga
      721 catatgttag aataaacagg tataaatagt gaaataaagg aatgacaagc aagagaagga
      781 ggaaaaaatg aaaagaagtg acaagcacgg caaaaatcga acaggcgctt atattgccgg
      841 cgcagtgatc ttaatagcag ctgcgggtgg cggttatttt tactaccagc actatcaaga
      901 aacccaagca gtagaagctg gagaaaagac agttgagcaa tttgtccaag ctttgaacaa
      961 gggagattat aacaaagctg cagaaatgac atcaaaaaag gcggcaaata aaagtgcatt
     1021 atctgaaaaa gagatcttag ataaatacca gaatatatac ggtgctgctg atgtcaaagg
     1081 acttcagata tcgaatctaa aagtagataa aaaagacgat tctacttaca gtttttcata
     1141 taaagcaaag atgaatacct cattaggtga attgaaagat ctttcttata aaggaacatt
     1201 agacagaaat gatggtcaaa ccacgatcaa ctggcaaccc aacttggttt ttccagaaat
     1261 ggaaggaaat gacaaagtaa gcctgaccac acaagaagca gcaagaggga atattataga
     1321 tcgaaatggt gaaccattag caacaacagg caaactaaaa caattaggag tcgttccaag
     1381 taaacttgga gatggaggcg aaaaaacagc caatatcaaa gcgattgctt cctccttcga
     1441 cttaacagaa gatgctatca atcaggcgat ttcacaaagc tgggtacaac ccgattactt
     1501 tgtcccattg aaaatcattg atggagcaac gccagaactt ccagctggag ctaccattca
     1561 agaagtagac ggcagatatt atcctttggg tgaagcagct gctcagctga ttggttacgt
     1621 gggagatatc acagcagaag atattgataa gaatccagaa ttaagcagta atggaaaaat
     1681 cggtcgctca ggtttggaga tggcttttga taaggatctt cgtgggacaa caggtgggaa
     1741 attaagcatt acagatgcag acggtgtaga gaaaaaagtt ctgatcgaac atgaagttca
     1801 aaatggcaaa gatatcaaat tgacaatcga tgcgaaggca caaaaaacag cttttgacag
     1861 tctaggagga aaagctggat caactgttgc gacaacgcca aaaaccggtg atcttcttgc
     1921 gcttgctagc tctccaagct atgatccaaa caaaatgaca aacgggatct cacaagaaga
     1981 ttacaaagct tatgaagaaa atcctgaaca tccattcatc agccgatttg cgacaggtta
     2041 tgctcctgga tctacgttta aaataattac agcagcaatc ggtctcgaca acggcactat
     2101 cgatccaaat gaagtgttga cgatcaacgg gcttaaatgg caaaaagaca gttcttgggg
     2161 atcttttcaa acaacgcgtg tcagtagtga tgtatcacaa gtagacttaa aaactgcttt
     2221 gatctattcc gataatatat atgcggcaca agaaacgttg aaaatgggtg agaaaaaatt
     2281 tcgtacaggc ttagataaat tcatttttgg tgaagacctt gatttgccaa ttagtatgaa
     2341 tccagcacaa atttctaatg aagatagctt taactcagat atcttgctag ctgatactgg
     2401 atatggacag ggcgaacttc taattaatcc tatccagcaa gcagcaatgt attctgtttt
     2461 taccaacaat ggcacacttg tctatcctaa attgattgca gataaagaga caaaagataa
     2521 gaagaatgtc atcagcgaaa cagcagtaca aacgatcgtg ccagatctga gagaagttgt
     2581 gcaagatgta aatggtacag cacattctct ttctgcttta gggattccgt tggcagcgaa
     2641 aactggtaca gcggaaatca aagaaaaaca ggatgtaaag gggcaagaga acagtttctt
     2701 gtttgctttc aatcttgata accaaggtta tatgatggtc agcatgttgg aaaataaaga
     2761 agatgatgat tcagcaacca aacgagcatc ggaactattg caatacctca accaaaatta
     2821 tcaataaaaa attgcttttt aagtacagta aaaggactgt ggcgttatcg tcacagtcct
     2881 tttttcaaat aaacggtttt cacgatgtct ctttcacaac ttctttttga tttactcaca
     2941 cttcataaac tttttttgta aaatatttga gaacaagtgg agaaataagt gtacttaata
     3001 aaatcacgat aactaaaggt gaatagtact gtggttcgat caattgactt tgctgtccaa
     3061 tttgtaaaat gatcaaagcc atttctccac gcgaaatcat accagcaccg accatcaatg
     3121 aactatgttg agaaaaaccg gcaatctgag caccaaaata acctccaagc aatttggtca
     3181 aaatcgctac aagcgtcaat atagaaataa agagtagttg ttcactaaag tcagaaaaat
     3241 caacttctaa tcctacactg acaaaaaata ctggtataaa tactgcatat cctaaagcct
     3301 ctacattgtg gtatacttct tctttaacct ttgtctgact gacagctatc ccagcaaaaa
     3361 aagcaccaat gacagagcta agcccaacaa gatccgctac gtaagacatt cctaaacaaa
     3421 taactagcga tacgatgata atagaagaat ttgcgtacac tttttctgca aatgaaagta
     3481 ggaaaggcgc aatccatttg actaataaga aaataaaaac aaaataaatt agctgttcaa
     3541 tcaaaacaag cggtaaagaa gcattgctag aagcttcccc cgttagaaaa gataggctga
     3601 aacttagaac taaaacgact aaaatgtcat ctaccacaga agcacccaaa attgttgatc
     3661 cttcttttgt attcactacg tttagttctt ttaatacttc tactgaaata ctgacagaag
     3721 ttgccgcaag aataatccca aagaaaatcg cttcatttcc cgaaacttgg aatgctttgc
     3781 ctgtcaacca gccaaaaaca accgggaaga tgatcccaag caaagcaacg aacataccag
     3841 gtcggaaata ttttttcaaa agagataaat cactttccag accagctaaa aacattaata
     3901 agatcacacc aatctccgaa aagtcatgga ctagaatatc aggtttgacc caattcaatc
     3961 cagcttggcc tagcaaaaca ccaacaagta attgtccaat cactgctggg atacctaatc
     4021 tccgcgagat atgtgagcca atcgttgttg aaatcaatat caggcataaa ataccaataa
     4081 attccattct catcattcct ttgtataaaa tatagcccga acttagcata acatacatta
     4141 ttgaaaaaat aggaaaatat actcacaaaa aaaccaacaa tccaaaagat ttttttggat
     4201 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
     4261 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
     4321 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
     4381 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
     4441 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
     4501 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
     4561 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
     4621 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
     4681 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
     4741 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
     4801 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
     4861 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
     4921 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
     4981 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
     5041 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
     5101 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
     5161 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     5221 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     5281 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     5341 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     5401 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     5461 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     5521 cactatttca tttcttggat tcatttcgat cgttccatct agccaatccg tattagggag
     5581 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     5641 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     5701 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     5761 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     5821 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     5881 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
     5941 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
     6001 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
     6061 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
     6121 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
     6181 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
     6241 tgcactacta gctgctggtc caccgccaat tactaataca tcataaggtt ctttttcaaa
     6301 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
     6361 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
     6421 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
     6481 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
     6541 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
     6601 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
     6661 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
     6721 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
     6781 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
     6841 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
     6901 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
     6961 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
     7021 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
     7081 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
     7141 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
     7201 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
     7261 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
     7321 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
     7381 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
     7441 tgcattaaat tcttcgattt ttttcccaat caatgacata gtaaaacctc caataataat
     7501 tatttattta taattattgt aatttagttg gagttgcaaa gtcaatggat attttgttat
     7561 ttaataaaga ttactcaaaa acgaaggtta attataaaaa tagaagaggt cgcaagaaaa
     7621 tgctactgct ggaaatactc gggataacgt tgagtaacag tttcctgttc tttcagcatc
     7681 atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg gttttttatc aactaaagcc
     7741 ttgagtaaat cataatgttg ttccatgatc gtttcccatg gaagcccctg aatggtcact
     7801 cgtaaccagc ggaaacgttc taaatgggta ttatggtcat ctaaccagtt ccaaacttct
     7861 tttcttcctg caatttcgaa acaagcttca tgaaataaat tatctgcacg gaaaaaagcg
     7921 cgttcgtcat ttattttgac tgctttttct tgttcttcta aaatcgtttc tagcactatt
     7981 cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga tttgctcttc caactgttct
     8041 cggacaaagc gagcattttc tgcagaaaca agattaattt tggaaacata cgtaccactt
     8101 tgaggtatcg tatacacgag ttcttgctga cgtagttgaa tcaatgcttc gcgaatggga
     8161 gtccggccga tagcaagatg ttcctccagt gccttttcag atattttcct tccaggttct
     8221 aagtcagcat agataatttg tttacgaatc gcttgataag cttgtgattg caagctgctt
     8281 ttcatcttta ttcctccaaa atcatttcta tgaacgtagt ataacagaag aggtctgaaa
     8341 aacaatctgg tatgtcacat taactagtaa atttgtataa atagtaaaat tttggttgac
     8401 atagcgattt ttattgtata aaattcactt gaatacaaaa actaatatgt tagtttagga
     8461 ggggaaaaat gattgattta aacaactgga ggaactttat ccctgaatta tctcaaaaag
     8521 aagtagaaat acctcatttt gaccgtgcat cattaagaga aaatggacaa aaaaatcctg
     8581 tctggatcca tttcggtggc ggaaatcttt atcgaggatt tcatgcagaa atcgctcaac
     8641 gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt ttgtgaaaca ttcgatgaag
     8701 aaataattga aagggcttac caagattttg atgatttgat tcttgaagtg gtcatgccaa
     8761 gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc tgacagtctg tattgtcatc
     8821 cagattgttc tgaacacttt gataagatga ttcgacactt tgaaaatcct agtttacaat
     8881 tagttacttt tgctattact gaaaaaggtt atcgattgaa agatacaaat aataaatata
     8941 taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa gccatcacat acaatgagta
     9001 ttcttgctgc gctactttat aaacgttttt tatctggagc ttggccgatt gcacttgtct
     9061 ctacagataa tttttcacaa aacggtagtc aattacgtac ttctttgcta acgatcgcaa
     9121 aagaatggca aaaggaaggt tatgtacaaa atacatttat ccaatactta tcgaatgaag
     9181 aaattgttgc gtttccatgg acgatgattg atcgtatcac cccgaatcct tccttgtcag
     9241 ttagcaaaat gttggcaaaa gagggctttt caaatatgtc gattttccaa accgcaaaag
     9301 gaaataatat tgctccattt gtcaatacag aggacatcca ttatttaatc atggaagatc
     9361 attttcccaa tggacgtccg aaattcgaaa aagctggggt attcttgact gatcgtgcaa
     9421 cggtagataa agcagatacg atgaaggtga cgacttgcct taatcctttg catacgtctt
     9481 tagctattat gggttgttta ctggggtata ggagcatagc tgaggagatg aaagatccag
     9541 atttatcgct gctagtttct gaaatcgggt acaatgaagg cctcccagtt gtggagtctc
     9601 caggtatcat tgatccaaag atctttctgg aagaagtaat tactgtgcgc ttttctaatc
     9661 caaatattcc tgatatgcct cagcgaatcg ccacggatac ctctcaaaag gtggcgattc
     9721 gttatggtga gacgattaaa aaatatatgg aaatagaagt taaagacccg cagacacttg
     9781 tattcatacc attgactctc gctgcttggc ttcgatacct tattggcatt gatgatcaag
     9841 gaaaaccatt catcccgagt ccagacccac tattgacaga ctttcagcat aagctttccg
     9901 atattcaact gggtacgcaa gatacatcac atattcatga gaaaatggct cctattttgt
     9961 caaataaaga aatttttggt gtagatcttt accaagcaga gcttggcaga aaaatagaag
    10021 aaatgttcat caagatggta gcaggaccga atgccgtacg aaaaacatta caggattact
    10081 tacaagaaca tggaggaaat ttatcatgga aatgactttt agatggtacg gatcacagga
    10141 agaaaagatt accttagatg aaatcagaca gattcctgcg gtcaaaggga ttgtaggcac
    10201 attgatggat ataccagtag gagaggtatg gcccagggag aggatcaaag aactaaaaga
    10261 agaaattgag tctcgtgggt taacactaaa agtaattgaa agcgtcaatg tgcatgatgc
    10321 cattaaaatt ggattacctt cccgtgagca gtatatcgaa aactataagc agacaatccg
    10381 aaacttggca gaatatggaa tagaagtcat ctgctataat tttatgcctg tatttgactg
    10441 ggtgaaatct gaccttgact accgcttaga agacggctca tccacgttag cttttatatc
    10501 tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc atcgaacatt ccagcaatga
    10561 atttgaattg ccgggatggg aacctgagcg cttagctcat atcaaatcct tgcttgaagc
    10621 ctatgcatca gtagacgaag aaaaacttcg agaaaatttt gcctattttc tccaatcaat
    10681 catcccaaca tgtgaagagg taggggtcaa aatggctgta catccagatg atccgccata
    10741 ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa gatttagatt ggttgtgccg
    10801 catagtagac agcccttata acggaatcac tctttgtaca gggagtattg cagaagatcc
    10861 agaaaatgat gtatatggga ttttagcaga attttgtcaa agagatcgaa tcccatttgc
    10921 acatgttcga aatattaaat ttttagctaa tcgagatttc tatgaggcac cgcataaatc
    10981 cgaatacggt tcattagaca tgtaccgcat actaaaaaca atgtatgaca acggatttaa
    11041 cgaatacatc cgtcctgatc atggaagaat gatctggaat gagacaggaa gaccaggata
    11101 cggcttatat gatcgagcat taggtgctgt ttatctaaat ggattatggg aagcaatcga
    11161 aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg taattttatt atgtaaataa
    11221 gaaggatgtt caaattcttg atacataaat ccggaggttg ttaataagca ttcggattta
    11281 tgtatcttat ttggtctagg aagtaacttg attttctttt ttcgattgat ccatgagtgc
    11341 ttcttctttt aattcaaaga tatattctga gatcctcata atcacttttt tccagtcttc
    11401 tggtgtgatg attcctttta aaatatagat ttttttgata tgagaaggca attgtttcaa
    11461 agggcagtcg ctgataacga tgttgcagtt ctccataaaa tcattttgta ttttgatcga
    11521 ttcatttgaa aaaatttgtt ctatcttaac gatcagatct ttactgatat aaaaatcacg
    11581 cgtatagttc acaccaatca caattcgcgg ctgttttttg tagcgatcat acaaatgtac
    11641 taggtcttca accatccatt gcacattttc cttttctaaa ataactggta acaagtgttg
    11701 attttgttct ctgaaatcca tataaaattg ttcaatggcg gtttggactt ctttcaacac
    11761 gctatgttct tgataaagca tgaaagtagg aaggggcgta tttggttgat tgaagttaag
    11821 aaatccatta taaagtttat ttcgtgcgaa tgccaatgag taaagaatct tttctttttc
    11881 aggtataaac aaatcaaata cagtacaaaa ggactccacc atttctcttg aataaagaac
    11941 atgaggaacg tttgcttttt ttaaaagttt gtattgctcc aagcttgttt cttccgattc
    12001 gattttcgtg atagaaagtc gagctaaaat attcactaag atgcgttgtt cgcgacaaat
    12061 tggcagatta ggtcgcaaaa tatctacaga gggatcaata aaaaagtcaa tatattcata
    12121 ctctgtgaat tctttttgta cgtctttcac agaagttgca ggaaaattct ctttacatat
    12181 ttttaataga aaatataatt tatctatcat accgctattt agtcggtcaa gtacttcttt
    12241 tttaaaaaaa gtattcatca taaaagccac tacttgcgga tcttcatcat aaaattctgt
    12301 agcagtactt gaaaagatgt tccaataaat atctaatgct gcatattgaa tttgtacctc
    12361 ttttccagaa aaagactttc tgccattatt agaaaaggag atagacaaac cgtacaatgc
    12421 taaaaatgca ttaatctttt tcattttcgt atacaaataa gaggcactat agtttgtttg
    12481 ctgacttaaa ctgataatcg aatttgtctg acagctgaac aaagaagtca agatctgata
    12541 actgactgat tcgttgcagt atcttccaaa tagacgataa tagcagtaag ccggatcaat
    12601 tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca atatgcagtt ctgttagatt
    12661 gagacgctca aagtcttgtt ctaattcttt gatgtatagt aaaaaatttg attcagacat
    12721 atggaaatga tctgctaaat attgtgtagt aataaagaaa cgatgttcag ataggaaaag
    12781 aaaaatatct aattttgtat tgcttttttt cttcaagaga aacgttttaa tcgcttcttg
    12841 agtttgcatg aaagaggctc ctttttactt aaattgttaa aacttgaaaa tattactgtt
    12901 tttttcataa atttagacaa
//
LOCUS       DAZKOW010000083        12747 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000083 DAZKOW010000000
VERSION     DAZKOW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.083"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG912_002189"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACG912_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0513485.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACG912_002190"
     CDS             complement(323..892)
                     /locus_tag="ACG912_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFQ0513486.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACG912_002191"
     CDS             1078..1980
                     /locus_tag="ACG912_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFQ0513487.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG912_002192"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACG912_002192"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFQ0513488.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG912_002193"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACG912_002193"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFQ0513489.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG912_002194"
     CDS             complement(5003..8323)
                     /gene="dnaE"
                     /locus_tag="ACG912_002194"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFQ0513490.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8554..8739
                     /locus_tag="ACG912_002195"
     CDS             8554..8739
                     /locus_tag="ACG912_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFQ0513491.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8801..9997)
                     /locus_tag="ACG912_002196"
     CDS             complement(8801..9997)
                     /locus_tag="ACG912_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0513492.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10167..11603)
                     /locus_tag="ACG912_002197"
     CDS             complement(10167..11603)
                     /locus_tag="ACG912_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFQ0513493.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11896..12705
                     /locus_tag="ACG912_002198"
     CDS             11896..12705
                     /locus_tag="ACG912_002198"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0513494.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4018 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttactcttgc tgtcatccac ccaataagca tctgacagaa
     5101 ctacttttcg ttgcgttttt tcgtgataca taactactgg tatatatcct gggtgtttct
     5161 gaaaaatcac ttgtaattgt ttcattatct cgggatcttc ccgttcttct gtaatacgca
     5221 aatagcaagt ttgtgaagga tactgggctt ctaactggtt aggatcagca aagctttctc
     5281 caattagttg cagttcctga ttatatttgc tgatttcgct ccgcccttga acaaagatca
     5341 ctcgatcaac ggcaaacaaa ttacgatatt tacgataaag agttggaaaa actgtgacag
     5401 aaatttctcc actactgtcg gttccttcaa caaaggccat cagttctcct ttttttgtcc
     5461 gaatttctct aacttccttg atcatcacta ataaattgac tggttgatta gggacgatat
     5521 ctgtaatcag ctgaacttgt ttagctaaac gaagacgatc aaacccttct gcaggatgac
     5581 ctgacaaata aatacctaag tactcctctt ccagcttcaa gcgttcttct agtgtatagt
     5641 cagcgacttc ttcttctttt aatgccatga ttcccagcaa gtcaagactt ccaccactgt
     5701 aaacgatatt ttggattttt ccttcgagat caagcatcaa ttgtttcctg ttaggagata
     5761 attcatcaaa aactcctact gctgtcagtg attgcagtaa ctctaatttc agccaacgct
     5821 tatcgatccg tatgaggaat tgatccactg actggaacgg gccattttgt tttctttcgt
     5881 ttaatatatc ttctacaaaa tcacgccgaa taccttttat cgctgtcagt ccgaatctga
     5941 tttcttcctt attgaccaac tcaaaactgg aattactttg attaatggac ggcgaggaaa
     6001 gtttgagttt atttttacgt gcttctgcaa tatattcttt caattttgat gtgttatgcc
     6061 gaacagaatt catcaggctg gtaaagaaag cacctggatg atgggctttt agatacgcca
     6121 tttggaatcc cacgaatgaa taagcaaatg catgtgaccg attgaatcca tagtttgcaa
     6181 atttttcgat atattcatat acggtcgctg ctgttttttc cgaatatccc tgttgtaatg
     6241 ctcctgatac aaaatgcgac ttttcttcat ctaatacgtc ttttttcttt ttactcactg
     6301 cccgtcgcag aatatctgac tgccctaatg taaatccagc catagtcgag gcgatctgca
     6361 tgatttgttc ctgatagacg atcactccat atgtgttttt caaaatcggg atcagtgaat
     6421 catctgggta gcgaatggct tcttttcctt tcttcctggc aatgaaggta tctatatttt
     6481 gcatagggcc tggtcgatac aatgcattga ctgcggcaat atcttcgatg ttttctggat
     6541 ctaatcttcg taaaacgttc cggataccag cagattcaaa ttgaaatact cctgaggttt
     6601 cgcctcgttg aaacaagagc agcgtttttg catcatttag cgggatctct tttaatcgga
     6661 tctcataccc ttctactcgc ttaacagctt tcaaagtaaa atcaataatc gataagttgc
     6721 gtagtcctaa aaagtccatt ttcaaaagac cgattgcttc tacctcattc atcgtatact
     6781 gggtcagaaa agcttcatca gaaccgcgtt ggagcggaac tagttcaagt agattcttat
     6841 cgctgataac tacacctgct gcgtgagtcg aaacatgtct tggcaatcct tctaaaatca
     6901 ctgcagtttt gaataaaagt tggttttttt cgcttgcctg aactaattct actaattgat
     6961 gcgaagtttt ataagcttgg gtcaatgtga ttttcaattg attgggtatt gcttttgacc
     7021 agcggtttgc ttcactttgc gataaaccaa acactctagc tgcatctcta agaaccattt
     7081 ttgctgccat tgtgccaaat gttgcgattt gtgccatatg atattgtcca tacttttctc
     7141 gaacataatt caatacttct tcacgtcgat tgtcaggaat atccaaatcg atatctggca
     7201 tcgtaaagcg ttcggggttt aaaaagcgtt cgaacagcaa atcatattct aatggatcca
     7261 catctgtgat ttctaacaca taggctacta atgaacccgc agcagaacca cgacctgcac
     7321 cggtaacgat tttgtttcta tgagcaaaag ccattacatc ccatacgatc aagaaataat
     7381 catcgaatcc catttgatgg atgacagata actcgtagtc caaacgttct tggtattcct
     7441 ttgtatagtc agtgattcgg cgttccactt gcgaatagca taactctctt aaatattctc
     7501 cagcttgttt tccattagga actggataat gcggaagtaa tttctgatgg aactggaatt
     7561 caaatgaaat cgattcagct aaacgctggg cgcctattac agcttgggaa agacccgctt
     7621 tttcgaatcg tttgatcatt tcttgttctg aaagaagata gacatttgct gttagtggta
     7681 aagatgcaga taaattttct aaaggtattt ggcttccctc tttgatatgt ttcatcactt
     7741 caagtgcaaa aagttcttct tgttgtaaac tagcgatggg atgtaatgca caaagcgaat
     7801 aattttcttt tatcacatac tgataccatt caggcaattg atcaggcgtg ttttctgtca
     7861 agccgatata aaaatttcta gtaaaaacat tttcaagtaa aaactgccaa tgaccgaaaa
     7921 agttatcctc tttattgaaa gcctcagaaa cagaatgatt tgccggaaca acaaacgcta
     7981 gatggtcggt ttctgtcatt tctgcaagtt ccaatttttc ttggatcatt tttcggctgg
     8041 aaagtttcat cagctgctga taacctattt catcttttgc gtacgcaagc aagccataat
     8101 ttttttcttc ataagtataa ctaagatgta atccaatgat tggctgtact ccttcttcta
     8161 cacaaatacg atagaattca ggtgcaccgt ataagacatc tttatccgtt atggctaatg
     8221 aagtgtagcc ttgttttttt gcttcttgta catattctcg aatacgtatc gtactttgta
     8281 aaagagaata agaagtaatt gtataaagtt gtgggaaaaa catcttgcat tccgcctttt
     8341 ttttcgttaa aattcactag cgttaaaagg tttgatcctt ttactttcat taagatagca
     8401 ggcgctgaag ttgattattc cacttcattt gcttgcttgt aatctgtagg aatgagttct
     8461 ttcgcaagaa ctcaaatttt attgagattt ggtctcgttt ctttacattc aaagaaaatc
     8521 tggtaaaatt aagatagaaa agaggtgttc tttatgcgat atatcgttac attattctgg
     8581 gctgtcgttc taggtcaagt agtcggttat attggtgctg cattaacaag cggaacatat
     8641 gattttactt tgacaacaat catttcattc attgcgggtg tcattatcct tttgattggt
     8701 gcagttgcgc caagaaaaga aacctctgcc cattcgtaaa aaaacagcca agagcttgag
     8761 atttctcaag ctctttttta ttttctactc ctcaccacca ttataatctg ctgcgaagaa
     8821 ctttacgaaa gttgaataaa gaaagaagaa tatttgacag aacaaacaaa cttgtcacaa
     8881 taaaaacagc actgtaatta taagcatgcg cgactgtcga tcctagcatg ggacctaaaa
     8941 cttgtccaaa gttgctaaac atctgattgt agctatagat tcggctgact ccttcttgag
     9001 gcgtaatctt actgatcagt gtattgacag aaggcatcaa agcgccagtt gaaaagccta
     9061 aaataaagcg aagtatacct aattggagcg gtgtagtgac gaatgccata ggtaaataac
     9121 agcaaaatga taaaatgagt cctccaatta gaactttttg attcccaatc ttatccccta
     9181 gttttcctag tttcggtgaa gaaaagacag cggaaacacc agctatcgaa acaatcaagc
     9241 cgctgacaaa aaggatattt tctgtacttc cgctcaattc acgaatatat aaggtcaaaa
     9301 tagggctgat cgtagtgatt cctatctgta aaatcaaggt cgtaatgaag agaccgatca
     9361 aaatagagag atgatccatt tttgaaaaaa tttctttggt gcttatcaga tcttttttct
     9421 cgattggatg gaaatcttct ttgactaaga aaatggtcaa taccgttgtg atcatcaaca
     9481 aagcccctgt gatcaagaat acattttcca taccaaacca ctgagccagt gcaccgccca
     9541 ttgaaggtcc gatcagattt ccagcaatcg ctccggtaga caatgttcct aacgcccatc
     9601 cgctcttttc tctaggtgcc tgtgaagcaa tcatcgctgt ggcatttgga atatatccag
     9661 acaaaatccc attccaaaac cgcatgatca aaagccaata cgcgtttggt acaaaagcca
     9721 aagatcccat tgttaatgtc atccctgctg ctgcacggat catcatgagt tttcggcctt
     9781 ttcgatcagc tagatttccc cagattggtg caaccacggc agatgcaaaa gcagtcacag
     9841 agattgctag tcccgaaaac aattcgattt gatcttttgg tgttcctagt tgctcaacat
     9901 aaactggtat gaatggcata actaaactaa tacttgcacc agtgaaaaaa cagccaatcc
     9961 acgcaatcac taaatttttt ctccaattaa tctccatcta tcatccctct ttcgtctctt
    10021 ttatcatacc tattttcgaa taaacaagca acgatctcgc cagtaaaaag agaaaaatcc
    10081 cgcaaaggtc attttatatg taacgttctt tttatgtaaa acaaaaagag atcagaacaa
    10141 atactgttcc aatcccttct ctttagttaa attgttgcta gaatgatgaa gttgataatg
    10201 aataaaatat caacgatcca caaagcaaca gaaacttgat tgtattttcc tttgattact
    10261 ttcacaattg tatagaaaat aaatcctgct gcaatcccat aggaaatgct atagcacaat
    10321 cccataaaga tcgatgcaaa gaaggccggt aaagcttctt ccatatctag ccatttgatg
    10381 tctgcaaaag aagccatcat cattactcca actaaaatca atgctggcgc agttgcttga
    10441 tttggtacaa tggcgatcag tggagagaat aaactactta atgcgaatag cactgccact
    10501 actactgaag tcaaaccagt acgtccaccg gccccgatcc cagctgcaga ttcgacataa
    10561 gttgttgtat tcgatgtacc aaagatagcc cccacagaag tagcgatcgc atcggcaaat
    10621 agtgctttat ccatttttgt tttgaaccca cgaccatctt ccaacgcgat ctcgtcttct
    10681 tttgagaaaa tacctgtgcg gcgtcctgta ccgataaatg ttccgatcgt atcgaaagta
    10741 tcagataaac tgaatgcgat gatcgtcatc aatacttgag gaatcttgga agaatcactg
    10801 aataaagatt gcatgccttc agaaccaaat gctgcaccga aagttgttcc taattcttta
    10861 aatgaattac ctaacgaatt cgtttgccaa tcaatggaac ttaaatcaac aacacccata
    10921 aaaataccaa ggactgtggt cgctacgata ccgataagaa tagccccacg gacatttttt
    10981 acaactaaaa tggctgtcag taccaaacca atcacagcca ataagatagg agcgttgtca
    11041 aagttggcta acgcagggac gattccacca tttatcgtaa catttgtcgc ttttccgcct
    11101 tcaacgacag agctcgaaat agctgactgg tctgctgaaa aagataagaa tcctgcattt
    11161 ttgattccga catacgcaac gaaaataccg atcccgccac caatcgcatg ttgaagactt
    11221 tcgggaatcg cccgaataat catttttcga atatttgtga cagtaataaa aatattgatt
    11281 aatccgcaaa taaacaccat tgccaaggct tgttgccaag aataaccaag cccaaaaacg
    11341 accgtaaaag tgaaaaatgc gttcaatccc ataccaggtg cctgagcata tggtacattt
    11401 gcgaacaatc ccattaccag cgtaccaata atcgatgcaa tgattgtcgc tagaaatact
    11461 gcttggaagg gcatacctga tgctgacaaa atcgtcggat ttacgaataa gatataactc
    11521 atggcaaaga atgtcgttac tcccgccatt atttcagttg atacatttgt attgtttttc
    11581 tttaaaccga aaaacttttc cataaaaagt tccactccct gaattttttt ctgatggaaa
    11641 actataataa taaaaaataa gtaaaatagg gcaaatccat cgaacaattt caaattatat
    11701 agacgtttca ttcgcttttc aacgtttttt taacaaaaaa agcgaaaata atacttaacg
    11761 ttcgtgttta gcttatgttt agctatttat atagccataa ctgtagtttt tgttaggaat
    11821 agctgattgc tcttgttaat tacctaaaat atgctagact aaaacaaatg ctagttgagg
    11881 aaggaaggac ccttattgaa taaaaaatta attgccatcg atctggacgg aactaccctc
    11941 aatcaagatt cgatgattac atctaaaaca aaagagactc taaaaaaagc aatcaatgcc
    12001 ggtcatcatg tcagtattgc tacaggacgc ccattccgaa tgagccatca gttttaccaa
    12061 caattagaat taacaacacc gatggttaat tttaacggcg cattagtaca catccctaat
    12121 cagcactggg atggtgaaag agaaacgttg atcaatcgtg aaatcgcttt tgaaatctta
    12181 tcgcaaaaaa agcagctaaa tttagatttt atcgctgctg aaaatcgtga tacttttttt
    12241 attgacagtt ttgatttctt tgatgaaaaa atttttgctt cttctcgtcc aggagagaag
    12301 aatttgcttt ctcctaaaaa tttgacgaca aatcctacat ctttgcttgt ccgaacagac
    12361 aaacgttttg caggagctgt atctgctgaa ttaacacgtc aatttggcag ttatgtcgat
    12421 gttcggacat ggggtggacc tactgccatc ttagagattg tttcaaaagg aatccaaaaa
    12481 gcaaaaggcg tgcaagagat tgctaactac ctgtctatcg atcaaaaaga cgtcattgcc
    12541 tttggtgacg agcataatga tttggaattg ttggattatg caggttgggg agtagctatg
    12601 gctaacggta ctgaccaact caaaggcatt gcaaatgatg tcactccttt gagcaatcag
    12661 gaagacggtt tagctgttta tctggaaaaa ctacttaaac tataatagag aattagattc
    12721 cacaaaaaag aggttgtgac aattttt
//
LOCUS       DAZKOW010000084        12635 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000084 DAZKOW010000000
VERSION     DAZKOW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.084"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(146..1111)
                     /locus_tag="ACG912_002199"
     CDS             complement(146..1111)
                     /locus_tag="ACG912_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFQ0513495.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            1345..2190
                     /locus_tag="ACG912_002200"
     CDS             1345..2190
                     /locus_tag="ACG912_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0513496.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            2381..3568
                     /locus_tag="ACG912_002201"
     CDS             2381..3568
                     /locus_tag="ACG912_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0513497.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            3574..3930
                     /locus_tag="ACG912_002202"
     CDS             3574..3930
                     /locus_tag="ACG912_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFQ0513498.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            3932..4270
                     /locus_tag="ACG912_002203"
     CDS             3932..4270
                     /locus_tag="ACG912_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFQ0513499.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    4346..4602
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4665..5699
                     /locus_tag="ACG912_002204"
     CDS             4665..5699
                     /locus_tag="ACG912_002204"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513500.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            5692..6378
                     /locus_tag="ACG912_002205"
     CDS             5692..6378
                     /locus_tag="ACG912_002205"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFQ0513501.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            6402..7250
                     /locus_tag="ACG912_002206"
     CDS             6402..7250
                     /locus_tag="ACG912_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0513502.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            7447..8220
                     /gene="sufC"
                     /locus_tag="ACG912_002207"
     CDS             7447..8220
                     /gene="sufC"
                     /locus_tag="ACG912_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFQ0513503.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            8233..9519
                     /gene="sufD"
                     /locus_tag="ACG912_002208"
     CDS             8233..9519
                     /gene="sufD"
                     /locus_tag="ACG912_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFQ0513504.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            9516..10751
                     /locus_tag="ACG912_002209"
     CDS             9516..10751
                     /locus_tag="ACG912_002209"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFQ0513505.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            10738..11208
                     /gene="sufU"
                     /locus_tag="ACG912_002210"
     CDS             10738..11208
                     /gene="sufU"
                     /locus_tag="ACG912_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFQ0513506.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            11213..12604
                     /gene="sufB"
                     /locus_tag="ACG912_002211"
     CDS             11213..12604
                     /gene="sufB"
                     /locus_tag="ACG912_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFQ0513507.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
BASE COUNT         4179 a         2366 c         2798 g         3292 t
ORIGIN      
        1 ttacgacctc ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccag
       61 tacaaaaaca tgaacagctg tttttgtttg gccgttctta ttacttgatg cagaacagct
      121 ttttcacaac ctattgaatc gattattaag cgattttttt agaaacaaat tttccatgtt
      181 tgctatgttc tttaattaag aaacgtacgt aatgagcaag aacgtagaag acaactacac
      241 ttgcaaacag acattccgca acttttaaga aatccaatac gctactatag atacctaaac
      301 tgttgaagta atgaacagta gtaaatatag ctgttgcaga gataacaagc gggaatgtaa
      361 aggctgcata gcttggataa aaacgaagct tgaacaaacg tggaagcata gacagcacaa
      421 cgaaatacaa tacttgagat aaagcgaaca atgctacgac aagataaaaa ttaggttcag
      481 caaagttttt aagataacct gttaggcaaa gagaaccagg tgctgcaacg atcgtaatca
      541 gcggacgtgt cggatgagcc atatttctta cgaaaaagac acgtttgatg atgatcggca
      601 ataagaacgc atagcagatc aatgaaatcc aaaagaaaat ttttccaata agtgggaaga
      661 agttcccggc agttacagtg atgaccccaa atccaacgta aatgataaac cagcttggat
      721 aaattgcttc aattgaaaga tcagctttga taacatggtg ataattgaaa taaaagacaa
      781 ggatcacttg gaagataaca gcgcacagcc aaatgaattt gacaattggt gcaatacttt
      841 catatgaaac aaaataagta caaataacca tgagtgacat cgtaaaagtt ggtgaaacgg
      901 aggcgatgat tggatcatgc aacgtttgtt tcgtgtgttc gaataagata aacaatttaa
      961 caaaaatgaa tatcataaga atcatagaga taccgccagt tagattgcct aaagcaacat
     1021 ggtgatagtc tttaaacaga tttccaaggg aagctaaccc aaggatcaac ccgcagatcg
     1081 ggatcggaat aattttcaaa aagttcttca tatataatat tctccttttt atttaataga
     1141 ttttagtacc aagatgaacg aatacattgt aaaatgaata ggaatgtatg attacgagca
     1201 agcagacagc tgattgtctt catctaagtt actagttgga tcagcaactg ttttgttgta
     1261 catttctatt atagatggtt ttttgtatat ttaaatcaaa tagtttttat agtaattatc
     1321 ataaaaagtt atggaagtgg tgccatgttt aagttattaa cgacttttcg agcagtatat
     1381 gaaacaagaa atttttccag agcagcagaa gtactgtttt tatctcaacc agcagtctcg
     1441 aatcaaatca aacagttgga gcgggaattg aatatcgatc tttttcaacg taacggaaga
     1501 aaagagatgc ttccaactaa gcaagccgac ttattatatg aacggacact ggttttgtta
     1561 gaagaatggg aagaaactca gcaaaaattg ctcaatgaac aaaatgaaga agaagtatgt
     1621 cggatcgttg cttcccacac ctttgctgtc tatcttttgc ctcagttgat gaaacatctg
     1681 atcaaaaggt ttcctaaagt gcattttgag atcgaaatca cgaattctca tgaatcatta
     1741 gaacaagtag cgaaacatga atccgatttt ggttttatcg aaaagccatt agagacttcc
     1801 ggcgtacaaa gatactcttt gatggaagac cagctagtca tcgctgggga cccaaaaagc
     1861 aaattgtggc ttgtaagaga agcaaactct ggtgtttttc attacacgca gcgttatttt
     1921 gaagagatga atatccaaga taagaagatg atcgtgaaaa gtaacgatgt aattgttgcg
     1981 ttgctgaagg aaggaatcgg tcgctcgatt ttgtctaagc gctctgttcc agaaaatgtg
     2041 ccaatgactc cgttgaattt ccatcgatat ttttatttca tccaacgaac caatctggtt
     2101 tctgatcagc tgctagagat tgccgaagag atccttcgat acaatgaaag cacagaaact
     2161 tctcacagcg aggagaatct cagctggtag ataattgcat gtatcaaaaa aagttctatg
     2221 atacactaaa gataagcagt aggcttttgt actgcatttg ctcaagcaac tgcacaaacg
     2281 gaaagttaat ttttcccaaa tgtctatgga aagactttcc attatctaac tggaccgcta
     2341 taataatcag cagtatggtt tttaagaaaa gggatgaaac atggaaaaaa aattaaaagc
     2401 ttcgaacgat aatcgtattg attatggcgt gattttacca gtctttctgt tatgtttgat
     2461 tggtctgctc tcattatatg tagcacttag ccatgatccg aacaatccag cagtcttgaa
     2521 aggcgtaggg atgcaagttg tctggtattt agttggagcg gcagcaatcg ttgtcatcat
     2581 gcatttgaat tccaaatgga tctggaaact gactccttac ctttatggtt tggggttagt
     2641 ggtaatggga ctcttgctca aattctatga tcagaattta gctgcttcaa cagggtctaa
     2701 aaactggttt cgattcggtg cgtttacttt ccagccagca gagttgatga agattgcgta
     2761 catcttgatg atggcactga tcgtaactaa acataatacg caagtaaaag aacggacgat
     2821 gaaatcggac ttttggctga ttgggaagct cttgatcgtg acaataccgg ttttagcatt
     2881 gatcatggcc caagatgact ttggaacgat gcttgtcttt cttgcgatct ttggcgggat
     2941 tttcctgatg tctggaatct cttggcgcat catcgcacct gtagtaatct tggcagtagt
     3001 cgttggagca ggaacgatct tcttggttac gactgaaggc ggaagagact tgctttataa
     3061 agttggattc aaatcttatc agtttgcacg tattgattca tggctggacc ctttccatga
     3121 tacgtcagga atgagttatc agccggccca aggacttttg gcaattggta ccggtggctt
     3181 gtttggtaaa ggattcaacg taagcaatat ctatgtgcct gttcgtgagt ccgatatgat
     3241 ttttaccgtt atcggtgaaa acttcggctt tatcggtggt gcattcgtca ttttcttgta
     3301 ttttatttta atctatcgta tgattcgggt gtgtttcgat acgaataatg aattttacgc
     3361 ctatatcgca agcggtttga tcatgatgct gctgttccat gtctttgaga atattggagc
     3421 aaatatcgga ttacttcctt taacagggat cccattgcct ttcattagtc agggaggatc
     3481 ctcgatttta gggaacatga tcggtatcgg gttgatatta tcgatgagat accaaaatga
     3541 agcacctgca agatcttcaa ggaggtaaga aaattgtaca ccttttactg gtacccgaaa
     3601 tgttcaacat gcaaaaaagc caaagcgtgg ttagaccagc atggcgttag ttatcaaacc
     3661 gttgatatga tcaaagaagt accgaaggcc tcaacgattg agacttggct agaaacgagt
     3721 caagtaccaa tccgtcgcct gttcaacaca agcgggatga aatatcggga acttggactg
     3781 aaagatcaag taggcgagtt tacggcttca aaagcgagcc aagtcttatc gacagatggt
     3841 atgctgatca agcgaccact gatcttacga gaggaccagc ttgttgcaat tggattcaat
     3901 gaaaaaattt atgaaggaga actgatataa aatggcaggc aagcatttga agaaaaagga
     3961 caacctttgg atcttattta atggaaaaga atactgtgtt ggtttgacaa atgaagcgca
     4021 agaagaactt ggagacatca cgtttgccaa tctgccaaaa gcaggacaat cgctaaaaaa
     4081 agatgaacct ctgatcgaag ttgaagcgga aaaagcggtc aatgaatttg ccagcccgct
     4141 aacgggtaca gtttcttctg ttaatgataa aatcggtgaa gatatcaata tcttgaatga
     4201 ttcagatgaa atgaatgcgt ggatcttaag tttcaaagat gttgatccaa aagaatttga
     4261 cgaattataa aatcagaaaa tcacttgaaa aaagttgaca ctgattcttc cagttgctat
     4321 aatcataagc aatcctatat gaaacgagaa agctttgaaa agaagagtac atgtcacgga
     4381 cgtttgaaga gagccctgtt tgctgaaaat gggtaacgaa aatgagatgg aagatggtct
     4441 tagagcagag taagcgagcg taagcaaact tactacggga gtgcccgtta cagcgcgaca
     4501 gtatacggaa agaagctaag tccaagtact ggaagaggct gttattgcga agtaacggta
     4561 aatcaaggtg gtaacacgga agttcaagct ttcgtccttt tgcttaaggc aagagggcga
     4621 aggctttttt gtttgaaaaa ttataagaga aagatgtgaa tggcatgtca ttaatccaac
     4681 taacgaatgt caaaaagaca ttcaatggga aaaacggaaa catccaagcc gtcaatgacg
     4741 tttcactaaa cgttgaaaaa ggggatattt atggaatcgt cggctattct ggcgcaggaa
     4801 aaagtacatt agtccgtctc ctgaatggac ttgagcttcc cacgagcggc gaagtgatcg
     4861 taaacgatca agatattaca aaattaaaaa acaaagaatt acgtacattc cgaaagaaaa
     4921 tcggtatgat cttccagcat ttcaacctgt tatggtcaag aactgtactt gaaaatatcc
     4981 agctgccgct ggaattagct ggcgtaccaa agagcaaacg taaagaacgg gcagaagaac
     5041 tgttgcgttt agtcgggtta gaaggacgcg gacaagcata tccttcacaa ctttccggcg
     5101 gacagaaaca acgcgtcggt atcgcacgag cattggcaaa tgatcctgag atcctgcttt
     5161 gtgatgaggc tactagcgca ttggacccgc aaacaacaga agaagtatta gacttattgt
     5221 tagcgatcaa taagaaattg aatttgacca tcgtcttgat cacacatgaa atgaatgtga
     5281 tccgcaaaat ctgcaacaaa gtcgctgtga tggagttggg gaaagtcgta gaagaaggag
     5341 acgtattgac tgtcttcaga caaccgaaaa aagaagtaac caaacgcttt gtacaacaag
     5401 acatcgaacc agaagctgat tcgacagaac tattagctga actcatcaaa gaaaatccaa
     5461 gcggccgttt ggcaactttg acctttagcg aaagaaatgc caacgagcca gtcatttccc
     5521 aagcgattcg acgctataac atcgatgtca acgttgtgta tggaaagatc aaacaagtga
     5581 aagaaggttc ctttggttca ttgactgtct tgatgactgg tgaaaccaaa gaattagatc
     5641 aagcagaagt gttcatcaaa gaacaaggag taggaattga ggtgatccat cgtggataaa
     5701 agcttttcag aaacatatct agattttaaa caaatcaacc cggaaacgat gaatcaggct
     5761 atccttgata cactatatat gacattaatc tcaatggtct tagtgacgat catcggatta
     5821 gttttgggat tgatccttta ctctattgga cgcaaaaaca aaccgtatgc tcgaatcatt
     5881 tatggaatcg tatcgatcat cagtaatatt ttccggtcaa cgccatttat gatcctgatg
     5941 gttttgatca ttccatttac taaagcaatc gtaggcacta tgctaggtgc gcaagcagcc
     6001 attccagctc ttgttttatc cgcagcacca ttttatgccc gtctagtaga gattgctttt
     6061 cgtgaagtca gccctggtgt actggaagca gcagatgcaa tgggagcaag ttactgggaa
     6121 atcatctgga aggtcttgat acctgaaagt attcccgcat tgatttctgg attaacagtt
     6181 acaacgatct cgatgatcgg atttactgcg atggccggcg cgatcggcgc aggtggactt
     6241 ggaggtcttg cttggcagga aggctaccag cgtggtaatc taacagtgac attcgttgcg
     6301 actttaatta ttttactaat cgtatttatc gttcaaggaa tcggtgattt cttaactaag
     6361 aaaacagaca aaagataata ggatataggg ggatgtagga tatgaaaaag aaaatttttg
     6421 gatttgcagc agcgcttttg ctgactgtag gattagcagc atgcggtaac aacagcgaca
     6481 gcaaagattc agcaaataaa gcagacgaca cgacattgaa agtcggcgct tcaccgacac
     6541 cgcacgcaga gattttggaa catgtgaaac cattattgaa agatgaagga atcgatcttg
     6601 aaatcgtcaa attcgatgac tatgtattac cgaaccaatc tttggaagaa ggcgacattg
     6661 atgccaatta cttccagcat attccttatt tgaacaaaga aatcaaagaa aaaggatatg
     6721 actttgtcaa tgccggtgct gtccacatcg aaccaatggg cttgtactct aaaaaagtca
     6781 aagatgtttc cgaattaaaa gatggtgcaa caatcattac ttcaaattct gaatcagact
     6841 ggggacgcat catcacaatt ctacaagatg ctggcttagt caaagtcaaa gatggagtag
     6901 atctggaaac agcaacattc gatgatattg aagaaaaccc taagaacttg aagttcaacc
     6961 atacgatcga cccatcactt ttagcttcta cgtaccaaaa tgatgaaggt gacctagttg
     7021 caatcaacgc aaacttcgct tacggcgcag ggttgaatcc attagaagat gccgtattgc
     7081 ttgaaaagga caactctcct tacgtgaata ttattgcgac tcgtaaaggt gatgaagaca
     7141 gtgacaaaat caaaaaactg attgaagtgc ttcaccgcaa agaagtccaa gaatggatca
     7201 cagacaaatg gggcggctct gttaaaccag taaaggctga tgcaaaataa ggtatggaac
     7261 aaaaaagatg agaaataagt ctgtaatggc ttatttctca tcttttttat acctattcta
     7321 atttagaaat caaaattgtt taataataaa taaaagatat ttgattttag taaggaaggt
     7381 cattgcgctt cattctcaat tagtctaaaa tgagtgagaa cgaaagagaa tttgggggta
     7441 taatgcatgt ctgtcttaga aataaaaaat ctacatgtgt cgatcgagga aaaagaaatc
     7501 cttaaaggcg taaacctaac aatgaaaacc ggagaaatcc atgccatcat gggacctaac
     7561 ggcacaggaa aatctacctt atctgccgct atcatgggca atccaaacta tgaagtaaca
     7621 gaaggcgaaa ttttgtttga cgggaaaaat gttttagact tggaagtaga tgaacgtgcg
     7681 cgacttggct tatttttagc tatgcaatat ccaagtgaga taccaggtat cacgaatgcg
     7741 gaattcatgc gtgcagcgat caatgcgaaa cgtgatgaaa acgacaaaat ttctgttatg
     7801 gcatttttga aaaagttaga tgaaaaaatg gctttactaa atatgccaga agaaatggct
     7861 gaacgttatc tgaacgaagg tttttctggc ggagaaaaga aacggaatga gatcctgcaa
     7921 ttgttgatgt tagaacctac atttgcaatt ttagacgaaa tcgattcagg tctggatatc
     7981 gatgctttga aagtcgttgc taaaggagta aatgaaatgc gcggagacaa tttcggtgca
     8041 ttgatcatca cccactatca acgattattg aactatatca cacctgatgt cgttcacatc
     8101 atgatggaag gccgagtagt catgacaggg aatgctgacc tagctaaacg tctggaagca
     8161 gaaggctacg ctggtatcag taaagaatta ggaatcgact ataaagaaga agaagcgtaa
     8221 gggggagcaa gaatgaaaga aaaaaactgg ctcgattatc ttgacgcggt aaatgatttt
     8281 tctttaagta aaggcgaacc tgattggatg agaaccttcc gtcaagacgc actagcgaaa
     8341 gcagatgaac tgccattgcc gcatatcgat cgggtgaaat tccatcgctg gtcgttattt
     8401 gatgtgaaag aaacacaaac catcagtgaa acaggcacga ttccggcatt tgatgcaatg
     8461 aaagacaatc ctgtgcttgt ccaacaaggt tcatggacga tttttgaaca actgcctgtg
     8521 gaattagcag aaaaaggagt catttttacc gacttgttca ctgcaatgat cgaatatcca
     8581 gaactagtcc aagaatatta tatgaaaaaa gcagtaaaca tgaacgaaga ccaattgact
     8641 gcactccatg tagcttttat gaacagcggt attttcctgt atgtgcctaa aaatgtcgtg
     8701 atcgatgaac cactcgagtc cttattcatt caagatggcg catccgatga gcacttcttc
     8761 aagcatgtgt tgatcgtggc agatgaacat agtgaatttt cttatctgga acgtttccag
     8821 acaaccaaag aacaagtagc aaaatcatca ggtaatatca tcgtggaagt catcgctaaa
     8881 gctggttcaa aaatcaaata ttcagccgta gatcaattag gagaaaatat tacttcttat
     8941 atgaatcgcc gtgggcacat tttacgagat gcttctgtcg actgggcaat tggtgtcatg
     9001 aatgacggtc atgtgatcgc tgactttgac tcagatttag caggagaagg tgcacacgca
     9061 gaagtcaaaa tcgtggcaat cagctcgggc agacaaatcc aaggaatcga tacacgagta
     9121 accaataaag cccctcatac gatcgggcat attttgcaac acggagtgat ccgagaaaaa
     9181 ggaacattga cctttaatgg tatcggccac atcttgaaag gggcaaaagg tgcagatgcc
     9241 caacaagaaa gtcgtgtctt gatgctttct gataaagcac gtggtgatgc gaatcctatt
     9301 cttttgatcg atgaaaacga agtaactgct ggacatgctg ccagtgtagg acgagttgat
     9361 ccagaagaaa tgtactatct gatgagtaga ggattgcata aagaagaagc agagcgctta
     9421 gtgattcgag gattcctcgg ttctgtctta acagcaatcc ctgtagaaca agttcggaaa
     9481 gaattagtcg aagtaatcga agggaagttg aatggatgat cgatgctaaa aagctacgca
     9541 atgattttcc tatcttattt caagacgtca atgatgaacc tttagtttat ctggataatg
     9601 cagcgacgac ccaaaagcca aaacaagtac tccaggcatt agcgtactat tacgaacacg
     9661 ataacgccaa tgttcatcgc ggtgtccata cgttagcaga acgcgcgacc catgattatg
     9721 aacaagcaag agaaaaagta cgggcgttca tccatgcaaa agaaacagca gaagtgctgt
     9781 ttactcgtgg tacgacgaca agcttgaatt gggtagccag aaccttcggc gaccgctata
     9841 tcaaagcagg agacgagata gtgctctctt acatggaaca tcattctaat gttatcccgt
     9901 ggcagcagtt ggcaaaacga acaggtgctg tcttgaaata tatcgaacta acagaagaag
     9961 gcttcctgga tatggaggat gcagcgaaaa agattacgga caaaaccaaa atcgtctcca
    10021 ttgctcatgt atccaatgtc ttaggtgtct tcaatcctgt tcaagaatta gctgaacttg
    10081 tccatgaaaa cggcggggtc atcgtagtag atggtgctca atctgccccg cacatggcca
    10141 ttgatgtcca agcactagac tgtgatttct ttgctttcag cggacacaaa atgtgcggac
    10201 caacaggtat tggtgtttta tatggtaaac gaaaatggtt agaagaaatg gaacctgttg
    10261 aatttggtgg cgaaatgatc gattttgtcg aattatacga cagtacatgg aaagaactac
    10321 cttggaaatt cgaagcaggg acaccaaata tcgccggcgc tatcgcatta ggccatgcaa
    10381 tcgattattt gcaaaaactc ggtatggaca atatccatcg atatgaagaa gaactagccg
    10441 cttatgtgtt gccaaaatta caagcaattg atggattgac tgtttatggt ccacaagacc
    10501 cgaattttcg gacaggagtc gttgcattca atttggatgg gttacatccc catgatgtcg
    10561 cgacagcttt agacatggaa ggggtagccg tcagagcagg tcatcattgt gcgcaaccgc
    10621 tattgaagta ccttgaagta tcagcgactg ctcgcgcgag cttttatttc tacaacacaa
    10681 cgcaagacgc cgaccgttta gttgaagcaa tcatggcaac aaaggagttt ttccaacatg
    10741 ggactatcta aattagacaa tttataccgt caagtgatct tggatcattc ttcccatccg
    10801 catcatcgtg gaagcttgac tgactcgaca agcaagatcg aattgaacaa ccccacatgt
    10861 ggagatgtga tcgaattgca agtcgatgta aaagacggaa tcatcaaaaa tatcgccttt
    10921 gacggcagtg gctgctccat cagtacggca agtgcctcga tgatgacaga cgccgtcatc
    10981 ggaaaatcaa tagaagaagc agaacattta actgtagact ttctacagct tgtccaagga
    11041 aaagaagtca aagatcccga ccaattaggc gatgctgcta tgcttggtgg tgtagctaaa
    11101 tttcccgctc ggatcaaatg tgcgacactt gcctggaaag cactaggaaa agcaatggaa
    11161 aacgatggta ccgcagcttt ccatcattac cacaaggaag aggagtgaga acatgggcgt
    11221 accagaatta gaagaatata aattcgggtt tcacgatgat gtcgaaccgg ttttcagtac
    11281 aggagaaggc ttgacagaag aagtgatccg tgagatgtca agaatcaaag gcgaaccaga
    11341 atggatgctt gaattccgtc tgaaatctct agaaacattt aacaaaatgc caatgcaaac
    11401 atggggcccc gacctttcag acatcgattt cgatgccatc aaatattacc aaaaaccaag
    11461 tgacaaaccc gctcgtgact gggaagacgt accagaaaaa atcaaagata catttgaaaa
    11521 gattggtata ccagaagctg aacgagctta tctagccggt gcttccgcac aatatgaatc
    11581 agaagtcgtg taccataata tgaaagatga attcgaaaag ttaggaatcg tctttactga
    11641 cactgactct gctttgaaag aataccctga attattcaaa gagtactttt caaaacttgt
    11701 accgccaacc gacaacaaat tagcagcgct gaattcagcc gtatggtcag gtgggacctt
    11761 tatttacgtg ccaaaaggcg tcaaagtcga tgttccatta caaacgtact tccggatcaa
    11821 tgcggaaaac acggggcaat tcgaacgtac gctgatcatt gttgacgaag gagcaagcat
    11881 ccactatgtc gaaggttgta cggcacctac ttattcaagt aacagcctgc atgccgccat
    11941 cgtagaaatc tttacaagaa aagatgcgta ttgccgttac acaacgatcc aaaactggtc
    12001 agacaatgtg tacaacttag taaccaaacg agcaaaagca tacgaaggcg caaccgttga
    12061 atggatcgac ggaaacctag gtgcaaaaac aacgatgaaa tacccaagtg tttacttaga
    12121 tggaaaaggt gcacgaggca ccatgctctc catcgccttt gcaggagcaa accaaatcca
    12181 agatacgggt gcaaaaatga tccacaatgc accaaataca tctagctcta tcgtctctaa
    12241 atcaatttca aaagacggcg gagaagtaaa ctatcgagga caagtaacct ttgccaaaaa
    12301 cagtgcgggt tcgatctctc atatcgaatg tgacacgatc atcatggacg aatggtcgaa
    12361 atccgatacg atcccattca atgagatcca caacagccaa gtctctttag aacacgaagc
    12421 caaagtctca aaaatctcag aagaacaact ctactatctg atgagccgag gcctgaccga
    12481 atctgaagca actgaaatga tcgtcatggg ctttgttgaa ccattcacca aagaacttcc
    12541 aatggaatat gcagtcgaac tcaatcgatt gatcagttat gagatggaag gcagtgttgg
    12601 gtaaacctgt tataggagct agaacccttg atata
//
LOCUS       DAZKOW010000085        12512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000085 DAZKOW010000000
VERSION     DAZKOW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.085"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG912_002212"
     CDS             <1..878
                     /locus_tag="ACG912_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0513508.1"
                     /translation="SLSQLLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPIKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKGDRRSKHLKKYWKLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
     gene            complement(1119..2735)
                     /locus_tag="ACG912_002213"
     CDS             complement(1119..2735)
                     /locus_tag="ACG912_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0513509.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            2853..3071
                     /locus_tag="ACG912_002214"
     CDS             2853..3071
                     /locus_tag="ACG912_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513510.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3266..3727
                     /locus_tag="ACG912_002215"
     CDS             3266..3727
                     /locus_tag="ACG912_002215"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0513511.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3920..4159)
                     /locus_tag="ACG912_002216"
     CDS             complement(3920..4159)
                     /locus_tag="ACG912_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513512.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4183..5706)
                     /locus_tag="ACG912_002217"
     CDS             complement(4183..5706)
                     /locus_tag="ACG912_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0513513.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(5724..5846)
                     /locus_tag="ACG912_002218"
     CDS             complement(5724..5846)
                     /locus_tag="ACG912_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFQ0513514.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(5876..6859)
                     /locus_tag="ACG912_002219"
     CDS             complement(5876..6859)
                     /locus_tag="ACG912_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFQ0513515.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG912_002220"
     CDS             complement(6883..7032)
                     /gene="rpmG"
                     /locus_tag="ACG912_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFQ0513516.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(7053..7430)
                     /locus_tag="ACG912_002221"
     CDS             complement(7053..7430)
                     /locus_tag="ACG912_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513517.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG912_002222"
     CDS             complement(7462..7641)
                     /gene="rpmF"
                     /locus_tag="ACG912_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFQ0513518.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG912_002223"
     CDS             complement(7656..7925)
                     /gene="rpsN"
                     /locus_tag="ACG912_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFQ0513519.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8448..10214)
                     /locus_tag="ACG912_002224"
     CDS             complement(8448..10214)
                     /locus_tag="ACG912_002224"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513520.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(10174..11928)
                     /locus_tag="ACG912_002225"
     CDS             complement(10174..11928)
                     /locus_tag="ACG912_002225"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513521.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(12038..>12512)
                     /locus_tag="ACG912_002226"
     CDS             complement(12038..>12512)
                     /locus_tag="ACG912_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFQ0513522.1"
                     /translation="FGHSISPGIFGLLSGIIFVYISYNYPRKGIFTIYTIVLLLFFTL
                     MGGAYLPWFISSISMAILADIILSVFGYDRAIPQVASWALMQLGSAAGQWIPIWFFTD
                     RFRQDWIDKGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         3938 a         2451 c         1871 g         4252 t
ORIGIN      
        1 tttctttgag ccagctgtta ccagaagtgg tgtgtttcga tgaattcaaa tcagtgaaaa
       61 atgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctataaaag
      181 tccgtgaaca agtgcgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacattg
      301 gtcgggcttt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
      361 ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gattccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgttat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatac caaacagaaa
      661 tcaaacgagc gttgcgcgtt ccctattcca acggccctat agaatgtttg aataaccata
      721 tcaaagtact aaaacgaatt gcctatggct atagaaactt tcaaaactac cgagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa tactacccaa ctgactaaag
      841 ccagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattatt
      901 ctattgaatt tcttcaccaa cactcattga cgaagagcca aaataagtaa tctaaccgac
      961 tgttaactaa accattgcag gtcccgttaa agatacttgt gcctgttgtc accgcttctt
     1021 tttcttgtcc acaagcgctt aacacactta ataaagcccc tgctattatt aataataata
     1081 tctttttctt attaaaaacc tcctcatcta tttccagatt agtaaatgta gtaatctgtg
     1141 ctttcttttt tctcaatact aataactaac ttatggggta aacaaatact cgtttgaccg
     1201 ggttttgaga tccatccagt tctaaccgcg atttgatcag gactattatc ttctttaact
     1261 cgaattcttc catctttaat ctcaacaata ttatattgtc ccttcgcagg atgataattc
     1321 acgagtttat gattgatctt atccaaatcg aagcgatcca tttccttgcc atcaattcga
     1381 acgatggcat atttgatagc atcttcagtt gtttcattct ccttttctat agagaataca
     1441 aataagggaa taaaagaggt aataatcaaa atgccaataa ttatgacatc gcctatcttg
     1501 acaccagctt ttcttaaaaa tttcattaac gatatctggc taaacatatt agactttttc
     1561 catggaaatg gtgaaatttt tcggaaatta tctgcattaa gagaatcagt taaacaaccc
     1621 tcaagttgtt ccttaataca ttgttgatcc atcttcatac caataaaaac tagtttatta
     1681 atgcgttcac caaacaacgg atgccaggct tccttcaatt cgggattttt ttgtataatc
     1741 atttgacact caattttagg taaactagct acccaataat ttaatgattc aatactttta
     1801 tagctacccg atgtttcaaa gatcgtcgaa aaatcactat gttgggcaaa ccaaagaaac
     1861 ccttttgctc ttgtaatatt gccaggaaaa tttttatccg taagatgggc aaatttttgt
     1921 gcgtcaagga gtaaccgatt tcgatagata aaacttgaga taccatattc ttctgtttca
     1981 ggaatatgat tttgatagcc aagctctaac tctttgaacc agcccactga ataggaagtc
     2041 tcatctatat taaaaagatt tgtatttaga attttcgata agtctacttc acctttaatt
     2101 gtcctaatta ttttggcatt ggactgtaac ttagcaatta gaccgttaat tttgtaaatc
     2161 gtcttggttg acactaagtc actattattt aaaagtaata tgttacaaca ctcgaattga
     2221 tcaattaaca agccacgaat atcctgttct tcttcaacta cattaccttc agcttttctt
     2281 tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac gttttacatc cactactata
     2341 ataatggcac caatctctaa tcgttgggtc aaatcaatgt ctaaagggtt tttagctaat
     2401 ataattgctt gaataattgg aattggtttg ctgattcctg aagcttcaat aacaatttga
     2461 ttcaagtcag aaaattgcac taattgatct agttcaacaa tcaaatcttc gcgtaatgta
     2521 caacaaatgc atccattcgg catcgaaaca agtttttctc cagttctttt aaaacctttc
     2581 ttgtcgacta ataccccatc aacatttact tcccctatgt catttacaat gacagcaatt
     2641 tcctttccgt gatcaccgta cagaatatga tttagaagta tcgtttttcc tgaaccaaga
     2701 tagccagtta agattgtaat aggtattttt ttcataggga acctctttct ataccttgaa
     2761 tttacacttt aagtgcaaca cataaaaatg agtcataatc gatatccaac tagtataagt
     2821 ttgtcgagaa cactcctaga aggaaaataa ttatgagcca gtatcgtcat cttactatac
     2881 tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt ttctattagt ataattggca
     2941 acgacattca gcatagcccg tttactattt tacatgaact gagaagaaac aagaaaaaaa
     3001 ctattcacct tccaaagcgg aaaaaaatta tgcttatcga agaaaaaaat gcggacgtta
     3061 tcacattcta aatgatccgt gagtttggtc aattgttcgt catttatttg tcgattttca
     3121 gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa catatggact tttcaattag
     3181 ttttatcacg atctaacgtg caatttgcaa tgatcttttc gatacagaac ccctctcaca
     3241 cggcaataaa ggcctgatcc gttcattgca ccatcgagga aaaacaagac atacaaaaaa
     3301 tcatgtcgag tctcgtggaa agatttgcct tactcattcg atccatggcc gtccaaaatc
     3361 cgcagatgat agaagtgatt ttggtcattg gaaagccgat attgtagctg gcaaaactgg
     3421 tggtacttgt ttagtgactt tgacagacag aaaatcatgc ttccttttag caggaaagaa
     3481 ggtccccaga aagaaagccg cttatgtgag cgagaaaatg attgaacttt taagcagtat
     3541 tccaaatgag gcggtcaaaa cgatcactcc agatagagga aaagaattct ccaatcattt
     3601 cgcagttaca gaagcattga ataatatgcc cttttatttt ccagatcctc atgcccttgg
     3661 caacaatgaa caaattaaaa tacgaataga ctcattcgag aatacttgcc aaaatcgaaa
     3721 gagatagcta cggtagataa ctcaacgata aatcagatga ttgaaaaact gaataacaga
     3781 cctagaaagt gtttagattg gagaacacct tatgaagtct tctttgataa agtgttgcgc
     3841 ttgatttgac aattcaagac ataataaaaa gcagatcaaa tccaaataag ggaaaaatga
     3901 tctactctgg tattatcaat taattggcca agctattatg ccgttttgct aaacgtctac
     3961 ttcttccagt attgaccttg ataaaacaac aatcaggatc ccgttttaat actctatgaa
     4021 ctgtgtcaat actaacatgt aaagttgcag caatatcagc taatgtactc ccatttaatc
     4081 tcatttgctt tgcaaatcga taacgccgat aagcatcaat attcttggaa tccatagaca
     4141 ataatacttt tcttaccatg tatttcctcc aaaaattgaa ttttaatgag ccatcatttc
     4201 ttgagcaatt tcatgtttag acaatgaagc tggatagaaa gtaggccaat tatgcaattc
     4261 attatagagt ttttcttgac tttctccacc aaagaaaata tggaaatgct ctgccttact
     4321 tggtgcaatt ccatgatcac taaattgaat atatttaaaa cgaccagcac ctgcatcatc
     4381 agtttcaaaa ttaaaacgaa caccacggtt tcctttttca taattcaaaa ttttgtatcc
     4441 aacatatttg tatgtgtatt gatgatgttc accgttgact acaaaatcaa tggtgttgtc
     4501 tttaatgttg atagtatcaa tatctgtctt atagccagta gtataataat ctttgtactc
     4561 ggcagctgtc atgtctttat tgatttttgc tttgtaatcg aaaacttcat ccaaaattcc
     4621 atcttttaac aacggataaa cagattgcca ttcgcctgta taatcactca attcgcgatc
     4681 cttcacatcg gcatctttaa agtacccatt agcaactgtc ttttcatcct tcgcttcttt
     4741 ttctggaact acctcttcac cagcaaccat agttgttttt tctaacgctt ttagattgtc
     4801 ttccattact gaaacataat cttcaccatt atccatttgt tcttttgtaa gactttcaag
     4861 aggattcagt acttccaatt ttatcccagc ttcgttggcc aaagtttttg caattttatc
     4921 attagcattc ttttcaaagt aaatataatt gatcttattt tttttaacat actcttttaa
     4981 ttcagctaat cgaccagaag atggctcttc ttcaggactt agtccagcaa taggaacttg
     5041 tttcaatcca taatccaaag ctaaatatcc aaaggctgcg tgttgcgtca caaaactttt
     5101 ttgtttcgct tctttaagac tagtggtata gtcagcatcc aaccgtttca gtttagtcaa
     5161 atacttttca gcatttgtct caaatacttt tgcttttttc ggatacaatt taactaactg
     5221 atccttgata tttgaaactt ccttaattgc cattttgggt gaaacccacg tatgaggatc
     5281 tagttcatga tggtgatctt cttctccgtg atcatgatct tcttcttcac tacctggcaa
     5341 taaaatcatg tcttttgttc cttctactac attaggccca gctttcttcc aactttcttt
     5401 agcttttggt acccatgatt ccatgttttc gttatgatat acaaaaacat cagcatcatg
     5461 agcttttgcc atatccttag ctgagggctc atagtcatga ggttctgatc ccgctggaac
     5521 cattaaatcc acgttcgctt catcgccgac gatgtttttg gtgaaatcat acattggata
     5581 gaatgttgtt acaatttcta attttccatc tttatcattc gatacttttg catcattagt
     5641 gctggagcaa gctcccaata atcctagagc tgctatccca ataagtccta cactgatttt
     5701 cttcattctt tttccttcct taattatgct aatttcttca ttttatttcg tttatgttct
     5761 ttgataattt ttaatgccct ttttctagtt ttaatattcg ggcttttaag tcgtcgatat
     5821 gcactagata tatccgtttt ctccattaga aattaccccc aatcactact tatcattatt
     5881 tgctttccgc cagtaattta tcaaaactct gtttgatcct ttcttcatcc aacatttttc
     5941 cgattagaat aatagtggtt tggtcttttt gtcgagtggt gtattgaaaa ttaacctgtt
     6001 cgtttacctc ttgcatggca actataaaat cctgctcttg taaagcaatc aatcctttaa
     6061 aacgatacag gctcccttgg ttggttaaaa tcagccagtc aatccattgg agtaaaaaat
     6121 ctttttttat gtcttgatct gaagttaaga gaattgattt aaactcatgt acttgatggt
     6181 gttgatgata gtatttgcca ttatagtgat tttctgatga atcactttca aaaactaagg
     6241 gatgattaaa ttttttcaaa ttataaaaat cctttttttg aatcgtttca ctatgtgtga
     6301 acattcgaat atcggataaa gggttaatac ttttgatttc tctctgtaat ttttttagtg
     6361 cagataaatt caagttttgt tttctagaca caaaaatgcg atctgccatg acaagttgct
     6421 taagggcttg tggttctctt tcaataagtt caaaactgtc actatcaata acagtcaata
     6481 aactatcgat atatactctt cctcttagtt gaggcgctgt taaaagtgtt tgaatgattg
     6541 gttggggatc tgctatacct gtagtttcaa tgatgatttg tgaaattggt tgttgatgtt
     6601 cttctattgt ttccagtaaa gaaatcaata ttgctaataa atcactgcga agactgcaac
     6661 acatacatcc attatttagt tgtaacaccc gttcctcgct atgaactaat aatttgtgat
     6721 caatcccaac ttcaccaaac tcattttcaa ttataataat ttcttccctg gggtagggtg
     6781 agacttgcaa ggcttgattg attagcgttg tttttccagc ccctaaaaac ccagaaacga
     6841 ttgtaactgg tatgcccatt ttgtcacctt tttaccactt agctatttca cttctttaaa
     6901 aattgcccgt ctgcgcaatt ttggcgaata cttttttaat tctaaccgtt cagggttatt
     6961 ccgtttattt ttgctggtga gatacagacg ttctccagtt tcgacacatt ccaaaataat
     7021 attttgacgc ataaatcttc ctccttcaat acttatcgat cttcaagata tgcttgaatt
     7081 tgttgtgcat cttcaaaagt taactcgcca gattccaatt gtgaaactaa attttctttc
     7141 atatcaacag gtatctccca ataaccatcg atacattgaa ataactcctc gtcatactct
     7201 tcaaaaattg gatagattct taaaaaatca tcaaatgata agccttggtc ctcatagtaa
     7261 tctttgacta atccattttc aattgcctgt tgggtcggta aacccgtttc agtaataatt
     7321 cctctctcta ccatcgaacg ataaaaagtg catttatcta gcgattgctg ggtcgtttca
     7381 agataatact ttaatccttc ttcaactaaa ggtgggtaaa taatttccat tttgattccc
     7441 tcctaaaagt tatatattaa actattttga agctccttta attactttct ttccattata
     7501 gtaccctttt aaagatacat gatggctacg tctataatca ccattcgctt cgtcaaatga
     7561 aatttctggt tttactacag tttgatgagt tcttcttaac cgtttcttcg ccttagatgt
     7621 ttttcttgct ggtactgcca tgatatttcc tccttttacc aacttgcttt ttttactcca
     7681 ggaatcaacc cttgatgggc aagttctcta aacttaatac gggacatacc gaactttcgc
     7741 atataccctc ttggacgacc atccgtctga tcacgaagcc tcagacgatt cggatttgaa
     7801 tctcttggca atttagcgag agcggtgcga tctcctgcgg cttttaattc ctgacgttta
     7861 tctgcatact tttcaatcat cgccatttgt ttctttgctt tagcaatttt cgactttttt
     7921 gccatatacc tttatactcc ttttattttg atttagacac ctcaaatcgt aatcgttacg
     7981 ttttgacact gatactttac caacaagagt tgctcttgtc aacaataaat aacatgaaat
     8041 aaaactcata tgaatagata actatttgaa aacgaattta tcggtacata gagataatca
     8101 aatcatttaa aagaatatgc agtttaggct ctgaaaaata agaattcact tttattagtc
     8161 caatcaaaca caaataccgt tttaagtagc ttttcctcta cttaaaacgg caatatgaaa
     8221 gaaactataa ctttttattc aatcttttat gtttaactgg tttatcgcca attttttaaa
     8281 actttgatta ttccttatat tataagaata gacattaagt gaactaattc aatcatactg
     8341 attctttcgt gtattcaaaa ttagatgctg tttaataaaa ttattctttt tgaaaaagaa
     8401 ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca tcgtgattga
     8461 gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag atttttgtat
     8521 atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt tccttgatcc
     8581 atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat aattagtact
     8641 gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt ttccgcatct
     8701 aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa agcccgtgct
     8761 atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc gaccaatgta
     8821 tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg agctgctttt
     8881 ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa aatacttgta
     8941 ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga agctggagat
     9001 acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc atacagtcta
     9061 gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa cgcagtcact
     9121 tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt atcatatgag
     9181 aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga tccctcttgg
     9241 atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata aaatatttca
     9301 agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc tagtaagtaa
     9361 ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc tactagaatg
     9421 actactgcta aactaacata tgagaaaata gaggataagg ccatgactga aaaagtactt
     9481 aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag tctgtcttga
     9541 acttttttag atagattaaa gctattaatc tcttgctgca attctatagt ttcttgaaat
     9601 tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt ttgggatttc
     9661 tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc taatcccatt
     9721 ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc cataccgccg
     9781 gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga catgatagtt
     9841 tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt tgacaaatcg
     9901 tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata taagttttca
     9961 tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac ggcaaaataa
    10021 agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat tataatgaat
    10081 gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt agttaaagct
    10141 tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat tgtcaagcct
    10201 ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat gtttagccat
    10261 taattcatta tgatttccac gttctactat tttcccttct gacaagacaa gaatttcatc
    10321 tacagattta atgctggata atcgatgagc tatcataata actgtttttt ctttcatcag
    10381 atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg cactggcttc
    10441 atccataata acaatctttg catcttttaa aattgctctg gcaatagcta tacgttgttt
    10501 ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc tttcgggaaa
    10561 tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt tacttgctgg
    10621 cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata gtttcgtatc
    10681 ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat ttaaaggctt
    10741 accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta gtttagctat
    10801 cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt gttctaaaac
    10861 taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat tttcaaatgc
    10921 aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt gcttcatttc
    10981 taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt gcatacttac
    11041 atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca acatcaacaa
    11101 gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta taggaactat
    11161 caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg ttttcccact
    11221 caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca aggctttaaa
    11281 tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat attctaccgt
    11341 ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg tattctctgt
    11401 cattttcttt aacaagaata caccaattac taacatggcg attagaacca atccaatttt
    11461 aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg cttgagcact
    11521 atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat caattgtctt
    11581 tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg ttaaaccgct
    11641 aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag aaaataagcc
    11701 agaaaaaata taaaataaag ctcctaaagt taaccaagca acagtttgaa ccgcataact
    11761 cttagctaaa tattcatctc ctacaataat tagagactga agaaatttgt aaatactgta
    11821 atatccatag actgttaata cgacagaaaa atcagaaaat aaagtggcta accatccgta
    11881 gtatttcatt ttagggacat aactaaataa ttttttgtat actttcattt tttcctcccc
    11941 cgcattcaat ttcttctctt attcgtttac aactgttaat gctttccgta atagcgtaaa
    12001 ttagagatgt acaaacagat aaaaggtaaa ctacgttcta ttttttgtat ttttttaaga
    12061 cttttcttcc aatcaataca ccaatcatag ataaacttgc tacaactaac actgaaatga
    12121 ttccccatat acctaccgca tagtggatca ttgcatccat tgttgcagca gattgtcctt
    12181 tatctatcca atcttgacga aaacgatctg taaagaacca tataggaatc cactgtccgg
    12241 cagcactgcc taattgcatt agagcccaag acgcaacttg aggaattgct cgatcatatc
    12301 caaatacaga tagaataata tcagctaaga tcgccatact aatcgaactg ataaaccaag
    12361 gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa aacaattgtg taaatagtaa
    12421 aaattccttt tctagggtag ttatagctaa tataaacaaa tatgattcca gataataatc
    12481 caaaaatccc gggggatatg ctgtgaccaa ac
//
LOCUS       DAZKOW010000086        12493 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000086 DAZKOW010000000
VERSION     DAZKOW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.086"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..375
                     /locus_tag="ACG912_002227"
     CDS             <1..375
                     /locus_tag="ACG912_002227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513523.1"
                     /translation="VKDNRNSIRKEVLHLIKFVKKTVAIPSEKKINESTRKLYRRANK
                     KALKLSAFLSSLSLKLSLILFISEHVSITDFLNLAKNPYLKGVRSILWQGLQSLACLE
                     VRTWFSYSSCRKIFLMMILSIN"
     gene            complement(324..893)
                     /locus_tag="ACG912_002228"
     CDS             complement(324..893)
                     /locus_tag="ACG912_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0513524.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="ACG912_002229"
     CDS             complement(967..2256)
                     /locus_tag="ACG912_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513525.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2514..3458
                     /locus_tag="ACG912_002230"
     CDS             2514..3458
                     /locus_tag="ACG912_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0513526.1"
                     /translation="MEEKIEESKQKIDQLIQELEKAVIGKRSTIQLMIVALLAGGHVL
                     FEDIPGVGKTLLVKALAKAVQGTFSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPI
                     FTTILLADEINRTTPRTQSALLEAMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTY
                     PLPEAQLDRFLFRLQIGYPSFDDELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANV
                     FVSPEVASYALQLVSATRSHAAIQLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQD
                     LVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQIPVPVR"
     gene            3472..4545
                     /locus_tag="ACG912_002231"
     CDS             3472..4545
                     /locus_tag="ACG912_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFQ0513527.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="ACG912_002232"
     CDS             4535..6706
                     /locus_tag="ACG912_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HFQ0513528.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG912_002233"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="ACG912_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFQ0513529.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="ACG912_002234"
     CDS             complement(9009..11195)
                     /locus_tag="ACG912_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFQ0513530.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG912_002235"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="ACG912_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFQ0513531.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="ACG912_002236"
     CDS             complement(11417..11857)
                     /locus_tag="ACG912_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFQ0513532.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12429)
                     /locus_tag="ACG912_002237"
                     /pseudo
     CDS             complement(11931..12429)
                     /locus_tag="ACG912_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
BASE COUNT         3520 a         2631 c         2217 g         4125 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aac
//
LOCUS       DAZKOW010000087        12363 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000087 DAZKOW010000000
VERSION     DAZKOW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.087"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..1663
                     /gene="treC"
                     /locus_tag="ACG912_002238"
     CDS             35..1663
                     /gene="treC"
                     /locus_tag="ACG912_002238"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFQ0513533.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(1723..2904)
                     /locus_tag="ACG912_002239"
     CDS             complement(1723..2904)
                     /locus_tag="ACG912_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFQ0513534.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            3156..4679
                     /locus_tag="ACG912_002240"
     CDS             3156..4679
                     /locus_tag="ACG912_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFQ0513535.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(4748..5476)
                     /locus_tag="ACG912_002241"
     CDS             complement(4748..5476)
                     /locus_tag="ACG912_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFQ0513536.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(5489..6769)
                     /locus_tag="ACG912_002242"
     CDS             complement(5489..6769)
                     /locus_tag="ACG912_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFQ0513537.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(6910..7512)
                     /locus_tag="ACG912_002243"
     CDS             complement(6910..7512)
                     /locus_tag="ACG912_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFQ0513538.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7757..7963
                     /locus_tag="ACG912_002244"
     CDS             7757..7963
                     /locus_tag="ACG912_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513539.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            8275..8994
                     /locus_tag="ACG912_002245"
     CDS             8275..8994
                     /locus_tag="ACG912_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFQ0513540.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(9075..9758)
                     /locus_tag="ACG912_002246"
     CDS             complement(9075..9758)
                     /locus_tag="ACG912_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFQ0513541.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            9976..12162
                     /locus_tag="ACG912_002247"
     CDS             9976..12162
                     /locus_tag="ACG912_002247"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFQ0513542.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         4465 a         2117 c         2384 g         3397 t
ORIGIN      
        1 aggtaataaa tcagttgaat ggaggtaaat atccatgaca tttaaagaca aagtcattta
       61 ccaaatctat cccaaatctt acaaagacag taatggtgat ggaattggag atttaaaagg
      121 aatcaaagaa aaactcccgt atctgaatga tctaggcatc gacatgattt ggctaaaccc
      181 tatttatcca agccctcaaa aagacaacgg ttatgatatc tctgattaca cagccatcga
      241 tcctatcttt ggtacgatgg aggaattcga acagctaata gccgaagcaa agaaataccg
      301 gatagaaatc atgctcgata tggtgctgaa tcacgtttcg acagaacatg aatggttcaa
      361 aaaagcacta gcaggggacc agcattatca ggacttcttc attttaagag aagagccaac
      421 ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca tttggagata cggggaaata
      481 ttatttgcac ttatatgatc ggacacaagc tgacttgaac tggcggaacc aagcagtaag
      541 agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa ggggtaaaag gattccgttt
      601 cgatgttatc aatgttatcg gtaaagacga acaattgaaa aataataaag aaaatgatgg
      661 caaacctgaa tatacggaca aaccaatcac ccatacgtat ctaaaagaat tgaaccaagc
      721 aacgtttggg acggattcca ctatcatcac agtcggtgaa atgagctcta cgacgatcga
      781 aaattgtatc ttgtatacag aacctagtag agaagaatta tcaatggtct ttaatttcca
      841 tcatttgaaa gtcgattatg aaaatgggaa taaatggtct actcctccat ttgattttgc
      901 agaattaaag cgactatttc atacatgggg agaaaagatg agtgagggta acggctggaa
      961 tgctctattt tttaataatc atgatcagcc acgtgcgctc aatcgatttg tagctatcga
     1021 tcagtatcga aaacaaggag cagagatgct agcaaccatg atccatttga accgtgggac
     1081 gccctatatt tatatgggag aagaaatcgg catgatcgac ccagactatt catcgatttc
     1141 cgactatatt gacgtagaaa gtttgaatgc ttataagtta ctccttgaac aaggattcac
     1201 agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc gataactctc gaacacccat
     1261 gcaatggtca gacacagaac aagcaggatt tactacaggg aaaccttggt taaaagttgg
     1321 aggcaagcta gaagagatta atgtagaaaa agaactatct tctgaagatt ctattttgtc
     1381 ctattacaag aaattgattc ggctgagaaa aacatatcca atcgttgcac aaggagatta
     1441 tcatgcttat gaagcaaatc atccggaagt ttatggcttt cttcgccaat ttgaaggaca
     1501 gcagctgctt gtattaacaa atttctatga taaagataca aggatcgcca tcccagaagg
     1561 attcacagaa gccaatattc ttatcgataa ttattccgat actgttcttc atcaaagtct
     1621 ggagctaaaa ccataccagg caattgcttt gctgaaaaat taaaacaaaa acacaaaacg
     1681 tcgttcctac gagctattct cgtaagatcg acgttttgtg ttttatttaa aataatcttc
     1741 ttcagaaaat gaagagtttt catactctcc tttatcgcca taaaatttat tgtagcgttg
     1801 tttgaattta cggaaaataa aagtaacgag taatttgact aacgtataga taggaatacc
     1861 aaaaataacg cccacaattc ccattagatc acccatgaca agtaaaacga ttaagatcgt
     1921 aatcggatgg atctgtaaac gatcacccat tactcgagga atcaccaagt caccgtggag
     1981 aagttgtacg gcaaaccaga caatgacaaa tttgacagcc ataaaagtag agtcttggaa
     2041 tgcaatgatc aaacctggaa agaaggcgat aaacggccca atatatggaa tgatacataa
     2101 caacccagca gaaatagcta aaacactagc aaattctaat ccgatcagta agaaacaaac
     2161 ccagtagact gctccaagga taaatgaagc aatgatttgc ccctttagaa atgaacccaa
     2221 ttgttgatta gcaatctttg tcaaattttt aaaatctgta cgaaatgctg gtgggacaat
     2281 cgctaaaact gactgataaa atttttgagg atctctcaaa aggaaaaaag cgacaattgg
     2341 accagtaaat aaagtaataa aaatagctgt aacagttgaa aaaatgttgc tgatccgttg
     2401 tgcgccactt tcccaataat ttccaataaa tccagctata tcagacgtca cgtcgtcaat
     2461 agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt ttctccaaaa attgtcggag
     2521 actctcttga aaatcttgaa acaaatctgg caaactactg atcaatgtct ttgcctgttc
     2581 ttcaagaaaa ggaataagcc atatgcttcc aagccccaat aaaaataaaa taatcacata
     2641 aacgataatg actgacaagt tcctcgaaag gtgcttgctc aaacgtttga tgactggttc
     2701 caaaagataa tacaagatca gtgcaaacaa taatgggggc aagatagtag cgataatgat
     2761 tccgacaggc ttgaatataa acgagatctg atggaaaaaa taaatgatca gcgcaagtaa
     2821 gataactatt cctaaaacat agtaagaggt ttttcctcct aaaaagcgga tcaaacgatt
     2881 cgttgtatct gtttcttttt tcactctttt tcctccttta tctataaatt cattatagac
     2941 aaaagcgggt ataaaaggaa aatgatagag gttgattgca aaaatatcgt caatcattca
     3001 tataaacttt ctcatcaatt aaaataagaa aacgcttgac agttctttta accatatgat
     3061 aaattaaatc ctgttgctaa aacgtaacta ttacggtttg cgaaaagcga atcggaacat
     3121 tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa acgattgata ggtatattgg
     3181 ccatgctggt tttagcaggt atgttaactg cttgcggggc aagtgggaaa gccgaggaca
     3241 gtaaagaaaa attgagcgtg atgacgacct tttatcccat gtatgatttt acaaaagcaa
     3301 tcgtcgggga tgaaggagaa gtagagttat tgattccagc agggactgat tcccacgatt
     3361 atgaaccttc tgctaaagac atggcaaaga tccaagatac agatatcttt gtttataatg
     3421 atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac attacaagaa ggcaatgtcc
     3481 atacgataaa agcgacagaa ggtatgcttt tacttccggg aagtgaagaa ggacatgatc
     3541 atgaccatga acacggggaa gaaggccata cacatgagct agacccacat gtatggctag
     3601 ctccaagttt agcaatcaag caggtagcaa acatacgtga tcagctgatt gaagcttacc
     3661 cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta taccgagaaa cttcaagcac
     3721 tccatcagct ttatcaagaa acctttaagc aagcaaaaca aagatctttt gtcacgcaac
     3781 atgctgcatt caactatcta gcgttagaat atggattgaa tcaagtatcg attgctggtc
     3841 tttcttctag tgaagaaccg tctgcggcga gaattgctga actgaaacac tttgttaaag
     3901 aacatggaat caactatatt tattttgaag aaaacgccaa agatagtatt gccagaacgt
     3961 tagcaaacga agcaggtgta tctttagaag tattgaatcc tttggaagga ttgacgaatg
     4021 aacaaataga aaatggagaa aactatcttt ctatcatgga agcaaacctc gaagcgttga
     4081 aaaaaacgac agagacagaa aacccgctag aagatacttt gacccccaaa aaagaaaaga
     4141 gcgtctacaa cggttatttt gaagacgagg atgtacaaga tcgctctctt tcagattggg
     4201 caggaacttg gcaaacagta gatacgtatg tagaagatgg gacttttgat cctgtttttg
     4261 aatataaaga aaaattgaat caggataaga cagcacaaga atataaagaa tattatacga
     4321 agggctatca aacagatatt gcttcaatca agatcagtga gaaagagatg gcatttacgt
     4381 ttaagaatgg aacaacgaaa cgttcaaatt atcgttatgt agggaaagaa atcttgacct
     4441 acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc agaagataag gaaagtggcg
     4501 cttttagata tattcaattc agtgatcaca cgattgcacc ggctgactcc gaacattttc
     4561 atttatatgc aggaaatgaa agtcaagaag tattgctgaa agaaatggag aattggccaa
     4621 cattttatcc agatacaatg gatggacgtg aaattgcaca ggaaatgctt ttgcactgat
     4681 aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt tagtcggtcc aaactattcg
     4741 gttcttttta tgaaatcaat ttctttaatc cacggataga ggctgggatg gtctcgccgt
     4801 ttgccaacgt gacttctctg gtttttttat taatcgaccg cacattttgt gtattgataa
     4861 cataggattt atgacaacgg ataaaattcc gatgcatttt ctcgatttct ttgatcttct
     4921 catagaattg taattgggca tgagtcaaat ggacttccag tttatgtggc gctcctagcg
     4981 aagtagaaaa atattggatg tctttgatcg gtaaaaattt gatctcatca ttaaactcta
     5041 ttttgaagat atctttttgg atttgggaca tttgaaaatc ttcgaaaatt ttgctcagga
     5101 tatgctcgac attttctttt aaatcataaa gatcttcttt gacgatataa tcagtcggtt
     5161 cgatgttgct ttttaagatt ttcatcgaca tttccgtatg ggaagtcaca tagattattt
     5221 ttcctaaagg atctaattca cggatcaatt taccgatcgc tacaccatcc atttcactgt
     5281 cttctagttc gatatctaaa aagtaaatac caaaagaaga agttctgatc ttggctgcat
     5341 tgagaagttc ttccggatca gaggtagaaa tatggacata actgtctaag ttttcaatca
     5401 tgattctatt tttgatcacc gttgataaaa cttctaattg ttttggatcg tcttcacaaa
     5461 tataaatggg aatcatatca ttactccttt attttatttg tatataaatc gtttgagaaa
     5521 aacgctggtt ttcaattgat gtttcaagga aaagataagg attggtcttt gtcaattcat
     5581 ccaagataaa caagccaagg ccttcgtttt ttggatttgt ttttgttgag aacccttttt
     5641 gtttcaactg gtttaacggt aatgtatttg ttgttgtatt gatcacacga atcatcacgg
     5701 aatgattctt cttgagtatg gtcaattcga tttttttctt ctttgcagca ctagctcctt
     5761 caatcgcatt gtccaagata ataccaagca tacgtacaag gtttaccgta tgtttcgtat
     5821 ccagttcgat tggctcagga aaatgaagct ggacgatcaa ccctttttcc tgtgccatca
     5881 tgagttttaa tgacagtaaa ctacggatct caggaacttt catattatcc aacatactta
     5941 gattggccgg aaaaaggttc atcatctggc gtgtaggtaa tatcgtttga tcataataag
     6001 accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg aagagaaaaa agcagatttt
     6061 ggtaatcatg tttgaattct ctcaagtctt gatagttttt ttctagatct ttgatataat
     6121 cctgttgcat cctgacttgt tgctctaata actgatcagc taatttctta ttagagattt
     6181 tccaataata aaccaagatg atcaggccaa cacacagata tagaatgaaa aaactcgtat
     6241 tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt ataaaaaata atgatatagt
     6301 atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg gcggtccaca gtgtgaaacc
     6361 aagtctctaa cgattttttt aaacaaaaag ctaataaagc agataaaaaa gcggataaga
     6421 caatgtgaaa atactgcaat gaatctcctg gttcaataac cgaagatttc aaaacaaaaa
     6481 aatcgccgat gcttgttaag tgatctccta aaagcgacgt caataaccca aaaggaagcg
     6541 cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa agtaagcatc agcattccta
     6601 tagcaattag agcagtaaaa atccctaaaa tcgaatagac ccatccaatc aaaatacaag
     6661 aaatcagaat gactcccgca aacagatatt tgtatcggat gtgatggtca atcaataaga
     6721 tacaaattgt gtaaatcaaa tattgcaacg ctatggacca taaaaacata aagtacagct
     6781 cccattgatt tttaccaaat tatagcacaa ttggcgtatt atttttttaa tttttcaggg
     6841 attttgaatt cagaactcat accaaagcag cagccagata caaaaaagtc agataagtta
     6901 gttaattgtt taatgattgt ttttttcatg aaagattcct ccaaaattca tcaagtagtt
     6961 tgtaatagga agcaacgtag ctatttctaa aacaatccca agtagcaaat aggtagaaaa
     7021 gtaagaagct gagaaaaata acgtaatgag agaaaaaatt agaaacatgc tgatcgcctt
     7081 ctttaataat gggggacgtt tctcgggttc aatgggattg attgctgtac ctcttgatcc
     7141 atagcgaagc agtaacaaaa agcagctgat taacaaaaag accatcattg ggaaggagat
     7201 agtcaattgc agatgtttca tcaccaaggg aacgccccat aaataaagta gtgtccacag
     7261 cagacaaacg aattcagagt cggaatgcaa tccataagca tatctgcgga tgcagatata
     7321 agaaagaaag gtgattcctg tttccgccca tgagcctgtc accagagaaa agactagaac
     7381 gaccgctagt tttccaagat tattcaataa aacttctaag acaaatttta cttggacata
     7441 caccatatct tcttccgatt tgtctccgct gaataaggct tctaataatt tgttcacaac
     7501 tttatctttc atctcatacg tacctccacc acaaatttta tgctaaaaaa aataaaaaac
     7561 gtcttttgtt tcataaaaag tggttttgac atcaaaaccg tcatgatctc ctaaaaaaat
     7621 cttcgttttc caagaacctt accaaaaaaa gtgacagttt tgatacgaaa agtacttttt
     7681 atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa attactgacc ttataaaagg
     7741 tgatggaggg attcttatgg aaaatgaaca attgtttgaa gtcggcgaaa catatgcatt
     7801 taaatctgat ttatttgaat caccaataaa aggaaaaata gaaaaaatct atgaaaatag
     7861 tgtgatgata cgtgtcatga aatgcgcagt agaagacaat gataccgctc ataatctgca
     7921 ctggcagtta gttgtacgca aatcaagtac aatagaagaa taaaaacaga taaaatgttg
     7981 agagcataaa gaggttgtga gtctcccgtt tagctctgag ttacaaggaa taatgttaaa
     8041 aaacagcttt tcatgtttta tgaatattgc cttgtgaccg aggtgttggc aggagaatac
     8101 tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac agcctttatt ttcatgaata
     8161 ggagttactt ttggctactt ttagagaagt agcttttaag gagcagataa agtcaaggct
     8221 tttatttcta taaatgaagt agtaaaatga agaacagatt ttattacagg aaggatgccg
     8281 ataattgtga tcattgttgc aggaacgatc ggtgcaggga aaagcacact aactgaaatg
     8341 cttgcccaag atttagaaac aaaacctttt tatgagaacg tggaagataa tgaagtattg
     8401 ccgctatttt attctaatcc agaaaagtat acttttttat tacagatttt ctttttgaac
     8461 aaacgctttt tggcaatcaa agatgctttc agccatgatg acaacgtact agaccgctcc
     8521 atctacgaag atagtatgct gtttcatttg aatgcagatt tagggcgtgt ttctgaagta
     8581 gaagtaaagc agtatgaagg tcttttagaa acgatgctga aggagttaga agaaatttcg
     8641 ccacaaaaga aaccagatct actggtttat attcgtgtat cctttgaaac tatgttagcg
     8701 agaatcaaaa aaagaggccg ggaatatgaa cagctagaac aagatcctga actctattcc
     8761 tactataaag aactcaatcg acgatacgaa gagtggtatg aacagtttga tatatgtccc
     8821 aaaatagtga tcgatgggga caaatatgat ttcgtagccg atccggcatg tggagaacaa
     8881 atcgtacaag aaatcaagat gcgggcaaag aaaatgacag aagaggcaga tgcccagtcg
     8941 atccgctaca ctacaaataa cccgaaagca aagggaatct ttacaactaa ataatataaa
     9001 aactaagcaa attgctcact cgcagatatt tctctgtgag cagcatcttg cttagttttt
     9061 ttgattcatt gagatcaaat agtgaaccga ttcgtatgat aaaattgcca taaatgaaat
     9121 gtctgagtga gggccatgat caaaaataaa atggaaacga tcaaaatcga atgggcgcca
     9181 aacagatgat gacagaaaac aaggacaata gctcctgcga taaaggcaag aacgacactt
     9241 agatagtgga aaaatcgatc aaaatctgct ttttctttag aaatacaata atcatataag
     9301 ttcgttgcta ccccttttaa atttccagtt gtaaataaat tagtgtaggc acgcccgttg
     9361 attttatcaa aagctaccca ctggatcgat gcagtaaaag caatcaaaat cgttactaat
     9421 gcgggatgct catggacggg tgtcaaacta acaaagaaaa aaatcactgc ttcataatat
     9481 agaacaaaca agcgccagaa atgtttttct ttttttctaa aataatcaat tagatacttt
     9541 gttagaagaa tccctaagaa aaagaaaagg gtagacaata cttttttacc aacacttagc
     9601 cattctccgt caaatgcttg gatgatcgat agaatgatat tgccagtttg agcagaggca
     9661 aaagcaccat attggatata agtataactg tccatggctc ctccgataaa tgtcagcaaa
     9721 agtcctattg tacgcccctc gtgaaaggaa gtctgcattt ttttcatcac cttttatgaa
     9781 tatttttgtc ttccttatct tagcataaac gattgaaaaa aaggaaaaat tttcagaaaa
     9841 ctaaaatcat atcgcttccc attttcattt ttagaaaggt ctgttagtat aaagaaaaaa
     9901 tagacaacga aactttaaat aaccaacttg aaataaacaa attcaaagaa gaaagatggg
     9961 aaggaaatca atcagttgaa tgaaaaagaa attctccgat tacaaaaaga attgaaacgc
    10021 tgggagaaca agtataaaaa gcttcaacaa gtctttcggc tacaagaaga aatcattgct
    10081 acctatgaag cggcttatgg cgaagcaccc ggacttattg aggaagaagt acaagtaaaa
    10141 gaaagtgaag gaaacagctc tttctttaaa atgatggaga aacgttccta taatcgcttc
    10201 aatcaagaac aaaaagcagc tatcacatat gatatgaaga aacatcttcg aatcatagca
    10261 ggagctggga gcggcaaaac gcaaacaatc tgtgcaaaag ctgtttatct gatgacgcaa
    10321 aaacaagtag acgaagaacg catactaatg atcactttta caaggaacgc tgcaaatgaa
    10381 ctaaaaaaac gagtagataa tttcagccag agaaaaaccg ctgtccatat cggaacattc
    10441 catagtatct tttttaggat atacaatgaa atttgtcgaa aatttcctga agtagcaatg
    10501 caaggaatac aaggagactt ttcaaaggat actgcacaaa aagtgaatgc tgttttgcaa
    10561 cagctgatac gtaaatataa tctttatata tttgatcaat atggagaaaa aacgattgct
    10621 tctcggttag actattggca gaatatgaac ctttcagtag aagaaatggt tcagttagtt
    10681 aaagaaaaat atgattccat tgataagaat gcacgacagc cgatatcaga acgactgtat
    10741 ggattgctca cggaactcca agaacaaaaa agaagccagc aattgctaga gttcaatgat
    10801 gtactccaaa atctgaaaag cgctttggag aatgaggaga tccagcatta tatcggccaa
    10861 aaatatgatt atctattcat tgatgaattt caagatacta atccacttca atgggaaatc
    10921 gtgaaacgga tgacaaagga aaacaggatc aagttgattg ttgttggaga tgatgatcag
    10981 agtatctatg gattcagagg atcagagcca gcatacatca agaactttga aaaagaatat
    11041 cctaccaaaa ctcttttctt attaactaat tatcgttctc gggcagctat tgtccaagga
    11101 gcaaatagat tgatcagcta taataaaaat gatcggatac caaaggcgat gattccagca
    11161 caaaaagaag caggtgttat ggaagcctat ttgttttctg atactaaagc agaaagtcag
    11221 tggctaatca aacaaattca gacatttatt caaaaaaatg ggaaatataa ggaatcaatc
    11281 atattatacc gatcagcttc acaaacgcag cagcttgtcc aatctctgct gaaaacgaat
    11341 attccttttg tactggaagc agatagtcca tatgaaggta tctttggtat caagtctttc
    11401 caacatttct atcaaaaaat tgttcattgg caaacagcac tgaatctgca aaagaaaaat
    11461 caggcctacc agcaattgct ccggcagctg atggcagact gctacctgaa aaaaagtgaa
    11521 tgcgatcaat acttttctcc taaatatcaa aatatagccg ttgcagatta tattttgtcg
    11581 ataagcccca atttaaaatc gaaatctgca gaaatccgag agtttgagga agcgttgcgt
    11641 aaaacagacc agcatcaaga aatctattcg cttgttcaaa gctatcttca tctgccacgt
    11701 atagctaagg aaatcgacca gagtgatcaa gaatggatac gagaagaagt aaagcagcat
    11761 gctactttca gaagactcca agccttatcg caagaaaccc aagcgacagc taaagaatta
    11821 aaaacccgct tgattgctta cagacaagga aaacttgatg cactatgtat ccagagcatt
    11881 cacaaaagta aaggtcttag ttaccgaaat gtctttttga ttggttgtaa cgaaggaagt
    11941 ttgccttata acggagcgac agaaaagaaa gtaatcgact ttaatgaaat aaaagcagaa
    12001 ccagtaacga cgatagaaga agaacgaaga ctattttatg ttgcgatgac tagagcaaga
    12061 aatcgtctgt atctatgtgt acctcaaatg aaaaattcaa gaaaattgaa agtatcgaga
    12121 tttatcaaag aaaccgggag tcgtgtgaaa aaaggaaagt gatagaagag agggtgtgac
    12181 agaagtggtc agtttcaaga aagaagaagg aatgtacgaa aattgcttct caaatttttg
    12241 tgaaatttca gcttattttc gaagaagcta tttctggaac acagtttatt cggaaaaggg
    12301 aggtgtgaca agaattttgt cacacctccc ttatttattt tgaaaatgag aatactatta
    12361 tca
//
LOCUS       DAZKOW010000088        12201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000088 DAZKOW010000000
VERSION     DAZKOW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.088"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..650)
                     /gene="frr"
                     /locus_tag="ACG912_002248"
     CDS             complement(93..650)
                     /gene="frr"
                     /locus_tag="ACG912_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFQ0513543.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(653..1375)
                     /gene="pyrH"
                     /locus_tag="ACG912_002249"
     CDS             complement(653..1375)
                     /gene="pyrH"
                     /locus_tag="ACG912_002249"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFQ0513544.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1511..2392)
                     /gene="tsf"
                     /locus_tag="ACG912_002250"
     CDS             complement(1511..2392)
                     /gene="tsf"
                     /locus_tag="ACG912_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFQ0513545.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="ACG912_002251"
     CDS             complement(2491..3273)
                     /gene="rpsB"
                     /locus_tag="ACG912_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFQ0513546.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3632..4111
                     /locus_tag="ACG912_002252"
     CDS             3632..4111
                     /locus_tag="ACG912_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFQ0513547.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4328..5419
                     /gene="phnW"
                     /locus_tag="ACG912_002253"
     CDS             4328..5419
                     /gene="phnW"
                     /locus_tag="ACG912_002253"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFQ0513548.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5412..5540
                     /locus_tag="ACG912_002254"
     CDS             5412..5540
                     /locus_tag="ACG912_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFQ0513549.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5544..6089
                     /locus_tag="ACG912_002255"
     CDS             5544..6089
                     /locus_tag="ACG912_002255"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFQ0513550.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6543..8234)
                     /gene="argS"
                     /locus_tag="ACG912_002256"
     CDS             complement(6543..8234)
                     /gene="argS"
                     /locus_tag="ACG912_002256"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFQ0513551.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG912_002257"
     CDS             complement(8654..9601)
                     /gene="arcC"
                     /locus_tag="ACG912_002257"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFQ0513552.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG912_002258"
     CDS             complement(9716..10735)
                     /gene="argF"
                     /locus_tag="ACG912_002258"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFQ0513553.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG912_002259"
     CDS             complement(10826..12055)
                     /gene="arcA"
                     /locus_tag="ACG912_002259"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFQ0513554.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3342 a         2529 c         2203 g         4127 t
ORIGIN      
        1 ataatttttt ctttttttaa tttttaaata aaagaaatat aaataaaaac agaggaagcg
       61 gaatgatccg cccctctgtt tttatgattt tattaaactt ccaataattc tttttcttta
      121 tcagcagcga tcttatctac ttctttgatg ctgttatctg tcaatgtttg tacgtctttt
      181 tctaatccgc gcaaatcatc ttcggtaata tcaccatttt tttgttgttt tttatattca
      241 tcgatcgcat cacgacggat gttacgaatc gcgatttttg cattttctgc ttcttttttc
      301 acatctttag ctaattcttt tctgcgttct tctgtcagtt gcgggataac taagcgaatg
      361 acatttccat cattggttgg actgattccg atatcacttg ccataatagc tttttcaaca
      421 tcttgaagaa tgctcttgtc aaaaggtgtg atcattaaaa cacgtgcttc tggaatttgg
      481 attgatgcca tttggttcaa tggtgttggc gcaccgtagt agttcacagt aatacggtcc
      541 aaaaggctag cgttcgcacg gcctgcgcgg atttgcccaa gttcacgttg aagattttgt
      601 gctgcttttt tcattttttc ttttgcttct gtcataattg catctgccat ttttatttcc
      661 cccttacagt tgttccgata ttttcgccta agattgcacg gcgaatattt ccgtgttcat
      721 ttaaattgaa gacaagcagt ggaatatcat tatccatgct tagtgaactt gcagttgaat
      781 ccatgacttg taatcctttg gcaataactt ccagatgagt cagttcttca aattttactg
      841 catttgcatc tactttcgga tcagcagaat aaacaccgtc tacattgttt ttagccatca
      901 ggataacatc tgcgccgatt tctgccgcgc gtaatgctgc tgttgtgtct gtcgagaagt
      961 acgggttgcc ggtaccgcct gcaaagataa cgatccgtcc tttttctaaa tgacgttctg
     1021 ctttacggcg aatatatggc tctgcgattt gacgcatttc aatcgaagtt tgcacgcgtg
     1081 tcggtacacc cacgttttcc aacgtatcct gtaaagctaa ggcattcata actgtagcta
     1141 acatacccat gtaatcagct tgagcacgtt ccatgcccat ttgtgctccg atttgtccgc
     1201 gccagatatt tccgccgccc acaacgattg ccatttcaac gcccagctcg tgaacttctt
     1261 tgatttcttg gatgatttct ttgattactg gtggtttgat tccaaatcct gcgtccccag
     1321 ctaaagcttc tccactcaat tttaagacta cacgctgata tttcggtgtg accatgttca
     1381 ttcctccaat attttctatt gccattttac catacttaga tgatttcgac actctaagac
     1441 tggtttttct tgttttgctt aaaaaaaggg agcgcacaaa ccgtgcgttc ccgtctgtta
     1501 ttcaacagaa ttattttttc acttggctca taacttcttc tacaaagtta tcttcgcgtt
     1561 tttcgatacc ttcgccaact tcgaaacgta cgaatgtttt aacagttgcg ccttttgatg
     1621 caacgtattt ttcaacagtc atatcaggat ctttaacgaa tggttggtcc accaaagcga
     1681 tttctgcttt gaatttcttc aagcgtcctt ctaccatttt ttcaacgata ttcgctggtt
     1741 tgccttcgtt caatgcttgt tctgtaagaa cagttttttc atgttctaat tcagcttctg
     1801 gaatttgtga ttcgtttaca taacgaggat tgatagctgc tacgtgcata gctacgtctc
     1861 ttgcaactgt ttcgtcagtt gttccatcta gaacagtcaa tacggcaata cgtccgccca
     1921 tatgcaagta tccaccaaat gcagcattgt cgtctttttc tacaacttca aagcgacgga
     1981 agctgatttt ttctccgata acttgagttg cttcgatcaa gtcagattca attgtacctt
     2041 tatctgtttt gattttcatt gcagcttcca tgtctgctgg tttgttttca gcaacaagtt
     2101 ctgcaatttc ttttactaaa tcttggaaca tttcgttttt agaaacaaag tctgtttctg
     2161 agtttacttc aacgattgct gcagtgttac ctttcacagc aactgaagcc aatccttcag
     2221 ctgcaatgcg gtcatttttc ttcgcagcct ttgccattcc tttttcacgt aaaagatcaa
     2281 ctgctttttc catgtcgcct tctacttcaa ctaatgcttt tttcgcatcc atcattccta
     2341 cgccagtcat ttcgcgtagt tcttttacca ttttagctgt aacgtctgcc atgtttaact
     2401 cctcctgtgt gtttttcttt ttaaaaaaag ctgcttcaaa ggagagtgag gcttgtcgcc
     2461 cagcctttga aacagctcca tcattcaaaa ttattctgct gaagaattgt cgccttcaac
     2521 aacatctacg atttcttcga ttgatgttgc tgtgttttct tctgtgaaat cttcttcaac
     2581 tacttgatct tcaccttggt ttccttcgat gaaagcatca gccatttttg aagtaatcaa
     2641 tttaacggca cgaattgcat cgtcgtttga agggattact acgtcgattt catctggatc
     2701 gcagtttgta tcaaccatag ctacgattgg gatatttaat ttgtgtgctt cttgaacagc
     2761 aatacgttct ttacgtgggt caacgatgta cattacatct gggattcttg gcatatcagc
     2821 gataccgcct aagaattttt caagacgttc acgttctttg ttcaaaccag caacttcttt
     2881 tttaggtaga acttcaaaag ttccatcttc ttccattgca ttgatttgtt tcaaacggct
     2941 gatacgtttt tggatagtat cccagtttgt caatgttccg cctaaccaac gatggttaac
     3001 gaagtattgt cctgcacgag ttgcttcttc tttgattgct tcttgtgctt gtttttttgt
     3061 acctacgaat aatgctacgc cgccttcttc agcaacgctc ttcatgtaat cataagctgc
     3121 atctactaat ttaactgttt tttgcaagtc aatgatgtag attccgtttc tttctgtgaa
     3181 gatatatttc ttcatttttg gattccagcg acgagtttgg tgaccaaagt gtacgccggc
     3241 ttctagtaat tgtttcatag aaattactgc catgtttgtt tcctccgatt tggttttatt
     3301 tttcctcttc ttatcttcta ccttgccggc aaactttttc aagcacctac cgacaatcca
     3361 ataagaatgt ggatttgctg atgcataaag caccctcagc tattatacag acaaaagatc
     3421 cgtatcgcaa gagcaaatat acttttctgc tcaacattca atagtttacc tgaaagataa
     3481 aaagaattca agaaaaacag tagatttctc tttgtttctt tattatcctt tttccataaa
     3541 ataattttag gtataaaaga cgaatattat tgggcaatcc ttcttttttt tgttatgata
     3601 catgtgaaat ctttcgtaaa ggagaaagaa aatgcgcaaa gctcaaagac atttgctgat
     3661 taaacaagtc attgaagaat tcccgatccg tacgcaagaa gaactattaa acaaacttgg
     3721 gcattatggc gttaccgcca cgcaggcaac gatttctcga gatatccgtg atctaaaaat
     3781 cgtgaaggca cctgatgaaa atggcgtttc ccgcttcgta ttgtttcgcg aaaaaggaac
     3841 tcctgagact aggactgagg aagaggatcg gctggttcaa atgattgaag acattgtatt
     3901 gaaagtcgaa cgagtccact ttttgacgat cgtcaatacc ttgccagaca atgctcattt
     3961 atttgcatct gttttagatg agatcaaacc tccctctatt gtcagtacaa ttgctggatt
     4021 cgatacgatc atggtcatct cggaaaatga ggaggacgca aaacgagtag aagaatttct
     4081 tcaccatcca actgaagtat cactttttta atcagagcac gtattcctaa tatggaatgc
     4141 gtgttttttt attttcaata ttttccttat tgaataaacc tttacactct ttacaaaaat
     4201 acatcatttt ttattcaaat ttttcaacac cttgattgaa tcacaataat catcatttat
     4261 attggatgtt aacaagagca gcttcttgct ctttgtgtat ttcaaaaaag aaatgaaagg
     4321 aaatcatatg tataaattat taacttctgg accattgacg acttctgaaa cagtgaaaaa
     4381 tgctatgctg atcgatcatt gtacttggga tcaagaatac aaagaaatta cacaatggat
     4441 aagaaaaaaa ttattggatc ttgctcaagt atcggaagga atatacacaa cggtcttaat
     4501 gcaaggtagc agctccttcg gggtagaatc ggtattaagt tctgtgatca ggccagaaga
     4561 tactgtactg atttgttcaa acggcgcata tagacaacga atgatcaaca tgtgcgaatg
     4621 tctgaaactg aactataccg tttatgaggt atcggaacat caaatcctcc aagctcatgt
     4681 gattacacag ctgattgaag agaatccgtc tatctccgca ctagcaatgg tccatagcga
     4741 gactacttcc ggtattttga ataatttgga agagattgct gctgtcacac aagaaagaga
     4801 tcttttgttt atagtggacg cgatgtcaag ctttggtggg ataccgattt gtgtagccga
     4861 actagggatt gattttttga tttccagctc aaacaaatgt attcaaggtg ttccaggctt
     4921 ttcttttgta cttgctaata aagcggaact ggaaaaacga aaaaatgtta gccgaagttt
     4981 gtcacttgat ctatatgacc agtaccgcgt gatggaaaac gagaatggca aatggcgttt
     5041 cacttcccct acccatacgg ttttggcttt tcaacaagcg ctgaaggaac tagaggcaga
     5101 aggaggaatc gaaactcgat attcacgtta tctcgccaat aatcatgtgc tacgacaaaa
     5161 aatgacagag atggggttcc agcctttcat tcaagaatat caggggccat tcatcacctc
     5221 ttttctttac cctaaggact ggcgatttga ttttaatcat ttttatcagt atttaaagca
     5281 aaatggattt gtgatctatc ccgggaaaat cagcgatcat gaatgttttc gtatcggaaa
     5341 tattggcgag atctatccgg aagatatttt ggaactgaca gcaattatca aagactataa
     5401 ggagaacgtt aatgattaaa actgtaattt ttgattgggc aggaacaact gtggactttg
     5461 gctgtatggc tcctgtccac gcttttagaa atgcttttct agaaaaagga acccaactga
     5521 ctgacaaaga aatccgctaa ccgatgggaa aattaaaatg ggatcatatc caacagcttc
     5581 tttccctccc ttctgtcaaa aaacaatgga tagctattta tggacaacta cctcaggaaa
     5641 aagaggtgga ggaattgtat caacaatttg aacattatct gtttaaagat ttggtcaagc
     5701 attctacctt gaaacccgac acattggaaa cgatcgaacg gttgagaaac gctgggatca
     5761 atattggttc cacgaatgga tttaatgctc ctatgatgac aatcgtagca gaaaccgcaa
     5821 aaaaagaaag gtattcacca gacttcatgg caacttttga agacgtcgaa gggtatggtc
     5881 gaccgtttcc ttatatgatc cataaaaaca tgcagtactt caaaaacaag tcggttgatg
     5941 aagtaatcaa agtcggagat acggtctcag atatccagga agggaaaaat gccggagtgt
     6001 tgacagtagg tgtaattgaa ggtagttcgc taatggggat ttcttatgat gagtaccaaa
     6061 aattagagat ggggaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc
     6121 cggcgctgat atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga
     6181 caagaatcca gaatgacttt tatacaaaat aagaggttgt gaaaaagctg tcctgtatca
     6241 agtaataaaa acagccaagc aaaaatagct cctcatattt ttcgtttggt taggcttatt
     6301 accgcagatg caagcttttg aacaccgttt attcgaaaaa aaaaagggag ctgtgacacg
     6361 acttttgtca cagtcccctt atttgcaaac aaacagtgtt taagagacaa cctctccact
     6421 aaacggtgtt cattcagctg actctcggta tttgctcaaa aaacgataaa atttgaaaag
     6481 ctattttctc ttttgtttct ctaatactcg gatcagaacg ctgatttcac attctctttt
     6541 tcttacatct tatctggtgc atgaagtcct agcaaacgaa ggtcttcttt caataataca
     6601 gtcactgcat aaactagagc taagcgtgct tctttttgtt catcatcagc taaaatcttc
     6661 gtatgagcat agtatttgtt gaatgcttgt gctaatttga ttgcatgttt cgcaatcact
     6721 gacggttcgt atttttcccc agcagacaag accgtttcag ggtatttttg aactaattta
     6781 accacttccc agctggtatc gtcattcaat gcatagtctg ctgtttctga agggacaaat
     6841 cctgcttttt ccaacaggct gactgcacga gcatgtgtat attgcacata aggtccagtt
     6901 tctccttcaa atcgaacaac ttcttccagg ttaaagtcaa atgtattcag tcgatcgttt
     6961 ttcaaatcgt ggaaaatgac tgccccaacc ccaacttgtt tagctacagc atctttgttt
     7021 tccaaatcag ggtttttctc gctgatttgt tctttagcag attggattgc ttcatttaat
     7081 acttcttcta ataagacgat ctttccttta cgagttgata attttttacc accttgcgtg
     7141 atcaaaccaa atggaatatg gtgcatgtcg tcagaccagt caaagcccat ttccttcaag
     7201 acagctttca actgtttaaa atgatagctt tgttcattcc ctactacata tagagattgt
     7261 ttgaagtcgt aagtacgttt acggtacaat gcagcagcta agtcacgtgt aatgtatagt
     7321 gtcgcaccgt ctgacttctt aatcaacgct ggattcaggt cataagcaga taggtcaacg
     7381 atctctgcac ctttatcctc gttcagcaaa tgtttttctt ctaaaagttt gacgatttca
     7441 tccatcttgt cattgtagaa agcttcaccg ttcaacgaat cgaaacgtac ttccagcaga
     7501 tcatagattt tattgaattc tttcatcgat tcatcacgga accattgcca taattgaata
     7561 gcctctttat cgccttcttc cagacgtttg aaccaagcac gagcttcttc atttaattct
     7621 ggttctgttt cagctacttc atggaattgg acgtataaac gaagcaattc attgattggt
     7681 tctgctttaa cagcttcttc tgtaccccat tttttgtaag cgacgatcag ttttccaaac
     7741 tgtgttcccc agtcacctaa atggttgatg cggattggct gataaccgat tttttccata
     7801 atgaaaccga tcgaattacc gataactgtc gaacgcaaat gtcccattga gattggtttg
     7861 gcaatattag gagaagacat gtcgattggt actgttcctt gattcccgat gttgctatcc
     7921 ccataatgtt ctttttcttt cactactgtt tgcaaaactt ttttgctgat aagttcttta
     7981 ttcatgaaaa agttcaagta aggtccgaca acttcgattt tttcaaagtt ggcagaatcg
     8041 attttttctg ctaaatctgc agcgatttgc tgtggcgctt ttcggtaaac ttttgctaat
     8101 gaaaaggctg gaaatgcaac atctccgtgt tcagctgatt ttggattttc tagtaattgc
     8161 tcgacttgtt ccagtgttaa atcatctttg acagcgtcat agacagcttt tgcgacaaga
     8221 tctttattat tcatctttat tcctcctaaa gtgttccctt gattgttaaa caaaaaaaat
     8281 ctctagcgac ggcatactta gtatgccatc actagagacg agatttcccg cggtaccact
     8341 ccaattgtcc taaaaaagga ccagctccat ctgatatcgg tagacgaccg tctgtttaaa
     8401 acagaattct ccaagtgcgc ttcgttgtat cttcttcatt cggcttccac cacttccgaa
     8461 ttcgctagcc aagaatgata caagtactct cttgttcaac aaatcttagt cttattaaac
     8521 tttcttgaat tatatcagaa aaaaaaacga aaggctagtc gtttcataaa aaaaaaatcc
     8581 agatggccta aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga
     8641 aagacttaac aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct
     8701 cgatattttc taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa
     8761 tagctgcttc tactttcgga agcatgcttc ctggagcgaa ttgcttttca tcaatgtatt
     8821 gtttcatttc tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga
     8881 catacacatt gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta
     8941 atttttctga agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg
     9001 tttctacaac aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca
     9061 gcgtttcgat gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc
     9121 tccatccacg tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt
     9181 tggcttcttc ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat
     9241 cattttcatc cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt
     9301 ccttcagtac ttcgcccatg gcgttttgta accagtaacc gatagatcct tctgtcattg
     9361 caacacatgt atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa
     9421 tcaataggtt accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt
     9481 cgataaattt cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac
     9541 ttgcatccgt agacaagatg gcattgcccc ctaaagcgac aactactttt cgtttggcca
     9601 tttcattcgt cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt
     9661 ttgtgatttt ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa
     9721 cgcgagggat aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac
     9781 gattttctgc ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt
     9841 ccatttcagt gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat
     9901 ggaaagctgg caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca
     9961 tttccatatt gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt
    10021 cacccattga tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa
    10081 catcgtcggt aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga
    10141 cgatttcttc ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa
    10201 ttgcacctgt gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa
    10261 cagtgatacc ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt
    10321 gtgttggatg ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta
    10381 gttcctctac cattttttga ctgaatccac ggaattcgat tccgtcaaac atacgtccta
    10441 gtactttagc tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta
    10501 gatattcagg atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg
    10561 tagatgtttt ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac
    10621 cacgtttttt caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt
    10681 ttgtaaagtc tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa
    10741 acattctcct ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac
    10801 agtaagactg ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca
    10861 tgcagcgtgg accaccacga cctcgtgaca attcacttga attgatttct aacactttga
    10921 tgccatagct acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac
    10981 ctggagcgat tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct
    11041 catctccatt accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg
    11101 tttcgtgtaa gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa
    11161 agacatccat cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat
    11221 ctaccattgt aaatacggta tccaaatgca tcattgcacg gttgttcgga attttgattg
    11281 ccaacacttt ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag
    11341 ctttcgcatt agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt
    11401 cgtctccgcc ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat
    11461 ttgcaaatct tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg
    11521 cttcaaaagt catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg
    11581 taaagtacaa gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat
    11641 catatagact gctagaacgt acttcgatat cttttgtacg aaccccagcc atgattttat
    11701 ctaccatttc ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat
    11761 tcgatgcaat atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg
    11821 cgtcaatcgc ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca
    11881 atgtcttcgc aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga
    11941 acaacagacg atccataata tctggtgtca aattttccac ttcttgtcca ggccgtttca
    12001 gtaaaactgt ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt
    12061 gttgcctcct tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata
    12121 attttgtctc attcaaaatg agacattcct cacattaata ttcgataatt aaataattaa
    12181 acgtttttct atttttaagc g
//
LOCUS       DAZKOW010000089        12200 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000089 DAZKOW010000000
VERSION     DAZKOW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.089"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..326)
                     /locus_tag="ACG912_002260"
     CDS             complement(162..326)
                     /locus_tag="ACG912_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513555.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            621..1949
                     /locus_tag="ACG912_002261"
     CDS             621..1949
                     /locus_tag="ACG912_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFQ0513556.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2151..2984
                     /locus_tag="ACG912_002262"
     CDS             2151..2984
                     /locus_tag="ACG912_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFQ0513557.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3000..3737
                     /locus_tag="ACG912_002263"
     CDS             3000..3737
                     /locus_tag="ACG912_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFQ0513558.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3892..4860)
                     /locus_tag="ACG912_002264"
     CDS             complement(3892..4860)
                     /locus_tag="ACG912_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFQ0513559.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5092..5925
                     /locus_tag="ACG912_002265"
     CDS             5092..5925
                     /locus_tag="ACG912_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFQ0513560.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5959..6798
                     /locus_tag="ACG912_002266"
     CDS             5959..6798
                     /locus_tag="ACG912_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0513561.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6824..7384
                     /locus_tag="ACG912_002267"
     CDS             6824..7384
                     /locus_tag="ACG912_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0513562.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7583..9304
                     /locus_tag="ACG912_002268"
     CDS             7583..9304
                     /locus_tag="ACG912_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFQ0513563.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9469..10611
                     /locus_tag="ACG912_002269"
     CDS             9469..10611
                     /locus_tag="ACG912_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFQ0513564.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10627..11838
                     /gene="thiI"
                     /locus_tag="ACG912_002270"
     CDS             10627..11838
                     /gene="thiI"
                     /locus_tag="ACG912_002270"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFQ0513565.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4155 a         2048 c         2535 g         3462 t
ORIGIN      
        1 taggttcgag tcctactgcc ggagtaaata aagcttatgc aaagaaaaag aaatccaatc
       61 acttgggttt ctttttcttt tattattatg atgaatcaag ctttctgttt ttgtcacgta
      121 tgttgtaatc tttacgaaag agtagagttg gctactcata atcaaccata cttcccatca
      181 tcgctaaata cgaaataaat cagcattcct acaccaatca aagcacaagc tgcaccaaga
      241 aaagggatga cattcccaaa gttatctgta ttcataagta tgccaccacc cacaaacaaa
      301 aagccgccag caatcaggtt tttcatttta ttcatctcct ttcttacgtt atagtatttc
      361 cttctttcaa tgagtatttt gaaagctatc ttattgtatg atgttcttct aggcctcttc
      421 tttattatac aggccatttt cgttatctca tagaaatatt cctttttatc tgaaaactat
      481 tgtataccgt atgacatttt tcttcatgaa gttgtaagta tattctcatt caaatgaaaa
      541 tttcatcata gaagagattg tatttctaaa ttcttaagag taaaatacga agtataactt
      601 ggatttggaa ggatgattgg atgaaagcta ttacgcctga tatgacaaca aaatttaaca
      661 aacagtttca agcaaatatc aaacagcagg cattgcagcg ggcagttgtg aagaacggaa
      721 ttactgcttc tgctgaaaat gtttcagcac atgtggagaa tacaccggta ttttctatag
      781 atttggctac ggggaaagtg gctaatcaaa tgcaatcggg gcgttgctgg atgtttgctg
      841 cactgaacac attccgtcat aagatgctga atcaattcca tttaaaagag tttgaattat
      901 cacaaaatta cacttttttc tgggataaat atgaaaaggc aaattatttc tatgaaaaca
      961 tcattgcaac aggaaaagag gcaatcgata gccggaaagt agctttctta ctcgctactc
     1021 ctcaacaaga tggcggccaa tgggatatga tcgtttcatt attccaaaaa tatggcgttg
     1081 tgcctaaatc ggtgatgccg gaaagtgcga atagttcaaa ttctcgagat ttgaataact
     1141 acttgaacaa attactgcgt aaagatgcag tagtgttacg gaaaatggtt gctcaaggag
     1201 aaacattaga cgctattgaa gaaaagaaag aagaaatgct agaaagcatt tataacttct
     1261 tagctatctc attaggaaca cctccaaaag aattcgattt tgagtaccgc gatgaagaaa
     1321 agaactatca tcttgaccga ggactgacac cacaaatttt ttatgataaa tacatcggtg
     1381 taaaattaga tgattatgtt agcgtgatca atgcaccaac aaaagacaaa ccattcaatc
     1441 gttcttatac agtagagatg ctaggaaatg tcgttggcgg gaaagaagtc aaatatctga
     1501 atgttgatat ggaaactttc aaaaaattag cgattgctca gcttgaagaa ggagaatctg
     1561 tttggtttgg ttgtgatgta ggtcaatctt ctactagaac tagtggcatc atggccttag
     1621 atgcttattc aatggatgat ctatttgata cggattttac aatgactaaa gctgaacgtt
     1681 tagattttgg agaaagtctg atgacccatg cgatggtatt gaccggtgtt gacttagtag
     1741 acgggcaatc aacaaaatgg aaagtcgaaa atagctgggg cgaaaaagtt ggaaagaatg
     1801 gcttctttgt tatgagtgat gcctggatgg acgaatatac gtaccaaatc gttgtaagaa
     1861 aagatttatt gacgaaagag caattaaatg cgttcaatga agaaccaatt gttctatctc
     1921 cgtgggatcc aatgggtgct ttagcataga cgagtatttc aatgaagagt atgtagtaaa
     1981 gataaaaaga gctggcttaa gccagctctt tttatcttta aagtccattc cctgtttttt
     2041 atggtccctg tgctataatt ctaaataaag gataaggatt ggaaataatc cttttatcat
     2101 taaaaatatg atgacgaagt agaataaaat tgggagtagg ggggaaagtc atggatatcc
     2161 aaactgtttt atctgatctg aaattaaatg gtaaagtgat tccagttgtt ggtggcgatg
     2221 ttaatcagac ctatcgtata aaaaccgaac atcgtgctta ttttttaaaa atccatccga
     2281 atgtaaaaaa aggatttttt gaagcagaag tagacggatt aaaagaactg tcagcattcg
     2341 tacgtgtacc ggatacgtat atgctaggtg aaacaagtga aggcgcttat ttgttaatgg
     2401 aatggatcga gccgggaaaa ggggatcaac gagatcttgc tgcagcttta gctaatctcc
     2461 atcagcaaac agcgccacaa tttggttttc gtaaagataa ctatttgggt acacttgttc
     2521 agaaaaattc ttttgaagaa gattggtgga cttttttctt taaagatcgg cttgaatccc
     2581 agatttcttt ggcagaagaa acgaatcgat ggaatgtaca gcgtcaagaa aagtatctgc
     2641 gatttaaaga acgtgtattg aaatcggttg aaccaaaaaa gatcactcca cgcctcttgc
     2701 acggagacct gtggagtggt aatgtgttct ttgaccagca agggcatcca gtttttgtcg
     2761 acccagctgt ttcttatggg aatcgggaac aagatattgc aatgagccag ttattcggtg
     2821 gtttccgacc ggaattttta gatgcttatc aaactatatt tccattagaa aagggatgga
     2881 aagaccgact gccgatttac cagttatatt atcttttggc acatttgaat atgtttggag
     2941 aaagttacgg ttcccaagta gatcaattac tagaaaattt ttaagaggag ggcgtgtttt
     3001 tggcaaaaaa atataccaaa aagcatgaag tttcatatta tgagtgtgac atcaaccaaa
     3061 cgatgacatt tccttcgatg ttaggagtag ttatcaaaac gtctgaagat caaagcgacc
     3121 agttgggaag aggcagtgaa ttcgtcaata gctttggact tacttgggtc atcacgaatt
     3181 attccatgga gattaatcgt ctccctagag tcggtgaagt aatctcagta acgactcaag
     3241 ccatggagta caataagttt ttctgttatc ggaacttctg gatccatgat gaagatggga
     3301 aagaattagt caaaatagaa tctgttttcg tattgatgga ttttgtgaat cgaaaaatga
     3361 gcagtgttaa tgaagagatc attgctcctt ttgagagtga gaagatcaag aaaatcaagc
     3421 gtcaggaaaa aattgaaaag attgattcgg gcttcatgtt gccatatcgt gttcgttttt
     3481 atgatatcga tagtaatcag catgttaata atgcgatgta ttttaattgg atcattgacg
     3541 tattaggata tgactttttg actacgcatg ttccagaaaa agtaattatt cgatttgata
     3601 aagaagtaga gtacgggaat gagatcgaaa gccattttga acaaatccct atcgaaaatg
     3661 gtgtcaaaac aaaacatgaa atccgaatgc aagaacagct gtattgtgaa gcaaatatta
     3721 tatggaaaaa taaataaaaa agaggttgtg acagaagggg gcaacttcaa gaaataagaa
     3781 gaaatttacg aaaattgttt ctcaaatttt tgtaaatttt cgacttagtt ttcgaagaag
     3841 ctatttttgt cacagcctct ctttttaagt ttagctatta gataatgatg tttagttttg
     3901 gataaacgaa atcaattctt cacccgacaa accatagttt acagcaatca atagttttaa
     3961 acgtgctttt tgactattaa tactgttaca gaaaataatc cccatttttt ctagttcttt
     4021 tcccccgcct tcataatcat aaacaggctc tgcgattcca ttgaaacaac gagaaacaag
     4081 tactacaggg attttttgga caagaagctt ttccaaagca gctaaggctt gtggagggag
     4141 attccccgca cctaaagctt cgattaccaa accgtctact ttcgtatggg cgatggcttc
     4201 caataagtcc ccttgcatcc cagcatatgc tttaacgatc gggatagtcc catctacagc
     4261 ggaaatgtcc agtcgctttg tttctaaaag ttcctgcaag aataaaattc gattcttagt
     4321 tactaagccg atgggaccca atgtaggtgt tcggaaagta gcaacatttg tggtatgggt
     4381 ctttgtcacg tagcgtgcag aatggatctc atcgttcatg accaccaaaa cccccttatc
     4441 tagcgcttct tcacaacttg cgacacgaat cgcactttca aaattataaa ggccgtcact
     4501 tccaagttca ttacttgaac gcattgcccc agtcaaaaca attggtaatt tgttgccaat
     4561 cgttgtatca agaaaaaaag cagtttcttc taatgtatct gtaccgtgtg tgatcaccac
     4621 tccggatata cctgatgcat aagcggacaa gatacgttct tttaatacca acatgtgaga
     4681 aggcatgatg tgtggtgagg gaagattgaa gatatcttca actaataatt ctacatgctt
     4741 ggggattgtg atttctgcat ctaataatgg gtttgccaca tcaggactaa caccgcccgt
     4801 gagcatgttt tctttcatgg cgatcgttcc gccggtgtgt agtacaagta tttttttcat
     4861 tttacagact tccttacgaa taattttgca tagccgttaa atcttattgt atactatata
     4921 aaagagtttt gctatcagtg aagaataacg aatagcctaa cttatcataa aaaagctgac
     4981 cttattaaaa tggaaaagac tcaaccttga cctcctaata caatggagta tctggttgag
     5041 tttcttttac aagaaagcag ccaaggaaat caatcacgaa ggtggccgga aatgatcaga
     5101 atcggattaa catcgtttaa agaacatgac aaattaactg gtaaaaagaa taacactctt
     5161 tatgaatatg cagggtttct acccttagtg gaattagata ctgcctatta tggaattccc
     5221 cgaaagtcct cagttgagaa ttggataagt gaagtacctg aatcatttcg ctttgtagtc
     5281 aaagtctatc aaggaatcag cggtcaagga gactggaagc cttattatca gaccgaagaa
     5341 gagatgattg cgcatttttt agatgttatg tcgccaatgg tagatgctgg aaagatgttt
     5401 tgctttttag tccaattccc ctcatcattc aaatgtacga aagaaaatgt ccagtacttg
     5461 cgcaaactta gaaagtggtt tgagggttat cctgtttcta tagaattgcg ggattattct
     5521 tggtacagcg atgaatttct tgaacgtaca agaaacttta tgtctagagc cgctttttct
     5581 ttagccatca tcgatgagcc acagttgctg aatacaacga ttccttttga tccatttgtc
     5641 accaatccga actttgctct ttttcgattc catggtagga atgcgcaagg atggcaagca
     5701 aatgataaag agtggcgtaa aaaaagaacg ttatatcgtt attcagaaaa agaactgcaa
     5761 gaactagcac atgaagtcaa agtagttgat tctaagacaa aagaggtagg tgtgattttt
     5821 aataataatt ctggtggtga tgcggctgaa aatgcattag cactgcaaaa aatattaaat
     5881 atcaagccag atggactcaa tccaacccaa atggatctgt tttaagggct tattttattc
     5941 aagaaagggg cgaagtctat gatcaaagct attttttttg atattgacgg aacattggtc
     6001 accaatcgtt caaaagcgtt ggaatcaaca aaacaagcaa ttgaatatgc tcggaaaaac
     6061 ggcattttat gcggtgtagc aacggggcgc agcccagtaa agatcaaaga aatcattgat
     6121 gaattggaat tagatatgta tgtcgtgtat aatggtcaac tggtttttac tgcagatcgc
     6181 acgattatcg accacccttt tgaacaaaaa gtattggaac atattgttga atttgcagat
     6241 gagaaccatc gacaaatcgt ttttggtgcg agaaatcggt tagatggatc aacgacgatg
     6301 ctattaggac aatcgatttt tatcaagcga ctagtcagct ttttaccgcg taaattcccg
     6361 gtacgtctga tgaaaaaaat cctccaggtt tttagccctc accgacaaaa ggatcgttat
     6421 gaaacgttag agatattaaa agaaccaatt tatcaatgta tattgctcag tccggagtca
     6481 gaacagaaaa agttagaaca tcgctttccc gagtgtacgt ttcagcgttc aaatacgtat
     6541 actgtagata tcatcccaaa aggtggttca aaattactag gcatccaagc tttcgcaaat
     6601 gctgtcgaaa ttgaaatgga agaaattatg gcattcggcg atcattacaa tgatatcgag
     6661 atgctgaaag gtgtaggaat tggagtggcc atggggaatg ctcagatcga agtaaaacat
     6721 gctgcagact ttgtgactca atcaaatgaa gaagatggga tatacgaagg actgaaacac
     6781 ttcggtctga tttattagtc aatgaaagaa ggaaaaaatc agtatggaag cagagacacc
     6841 ttttcaaaaa gtagaaacat tccacaaaac ttttgatcca agaagaccag agatacccac
     6901 ggcttttaca gaaaatgaag ctcatacacg tgctgcgttc aaaatagaag aacttgttga
     6961 atttgttgca gctacttcac ctaataaaga acaactgcat gaagctgtta tgctgctgca
     7021 cgaagcatta gataaagcag aagaaaaaat acttggtaaa acacagtggg gaagtgatcc
     7081 gctggttgaa caagtagatg cactgacaga tatcctttac tttacttatg gttctttttc
     7141 attactagga atagtgcctg atcgaatatt cgaaatcgtc catcaggcaa atatggggaa
     7201 attatttcca gatggaaaac ctcattatga ccctgtaaca aataaagtgt tgaaacctga
     7261 aaactggcag atggaatatg ctccagaacc taaaatcgcg gcagaaatcc aacgtcagat
     7321 tcaagaagcg attgctaaga agaaatccgc tgatataggc atgtgcacaa atgaaaaaga
     7381 gtgatcagag tttcactatt tttgttatct ttatacgatg gaaaaacaaa agattttcgt
     7441 ttaagttagt gaatgcatga aaatagtatg ctataatcta gaatagatgt cttttgcacg
     7501 agagacgtat tatttggatt tatgccaagc agaacgccat ctgttgcata aaagatgcat
     7561 actgaaaacg gaggaaccat aaatgggaaa taatgttatc atcggaatcg tcattgcaat
     7621 cattatagtc gccattgtcg tctttttggt tagttacttg atgaagaaaa aaaaccaaga
     7681 tcgattggat gccttagaaa aacgcaagga agaattattt gacttgccag tgattgaaga
     7741 agtcgatgat gttaaaaaaa tgcatctggt tgggcaaagc caaaatacct ttcgtgaatg
     7801 gagtcaaaaa tggaatgaca tatctacttc ctcgttcgct gaactagaaa gtcaaatttt
     7861 cgaagtggaa aacctcaatg aaagttttcg gttcttaaaa ggaaaaaaag cagttgttga
     7921 agcagaagct acaatggacc aaatggagaa acaagtgacg gaaatccgtg aaggtctaaa
     7981 ggaattgcgg gaaacggaag aacgcaactc actagctgtt cagcaggcac ttgacgtcta
     8041 tgaagaactg aaagaaaaag tcagagaaga cgagcatgaa tacggaccag ctctttttga
     8101 attgcaaaaa cagatcaaag tgatcgaaaa agaatttacg cagtttgtga ctttaaacac
     8161 ttctggagac cctgttgaag cccgcgaggt tttggataaa gcagaaaaac atacgtatga
     8221 agtggaagct ctgatgaaaa aaattccacc gctgtatgag gagctccata ccactttccc
     8281 tgaacaactt gaagaaatca gtgatactta cgagaaattg atggaagaac aatatgtatt
     8341 cccagaagaa gatcttgctg aagatatcgc aaaagtaagt cgaagaattg aaaattcatt
     8401 agccaatctt gaaaaaacag aagttgaaac ggtcgaattt gaaaaccgtg aaacagctga
     8461 tttgattgat tcgttatatg atatcttaga acgggaaatg gaagctcaga gatatgtaaa
     8521 aactaaccaa tcaacgattg cagagtatat caaacataca acaaagaata accgtcaatt
     8581 attgattgag ctggatcaca catctcaaag ctatacattg aaccacaatg aaattggccg
     8641 tgtacgaggt ttccaaaccg aagtggaaga aatggaacgt caaaatgaac agatgattcc
     8701 acagatccgt caacacgaaa ttccttattc agaagtgcgc acgttctata aaacagtctt
     8761 caaagtctta gaagatatcg aaaagcaaca agtagagatc gacgactcat tacatgaatt
     8821 gagaaaaggc gaaaaagaag cgcaagaaaa gatcgataca ttcgaattca aattgcgcag
     8881 tttgaaacgt tttgtagaaa aacaacgttt gcctggcttg ccgaatgatt acctagaatt
     8941 cttttttgtt gctactgacc gtattgaaga actaagtgtt gtcttgaata aaattcgagt
     9001 aaacatggaa gaggtcaatc gtttagttgc tctatgtgaa gaagatctag aattattaga
     9061 taagaagaca catgatttag tagatgcagc agcattaacg gagcaaatgc tccaatatgc
     9121 aaatcgttat cgtcatacac acgaagaagt gagagaagca attgataaaa gctactattt
     9181 attcaataaa gaatatcact atcaagaagc attagacgaa atcggtacag cattagagcg
     9241 agtagaacct ggtgctttca aacgcatcga agatttttat tttaatcatc cagacttagt
     9301 ataaagcaaa aaagttagta aaccagcaag agcaagacgg attattgtct tgttcttgtc
     9361 tttttttgta tatttgaaaa ttgaaacaag tgaaaatttc acctgctctt gaaagcacct
     9421 tggacaaccg ttataataga aaagaatttc aaaaaagagg ggtttcttat gatatatttt
     9481 gataacagtg caacaacccc aatctttccg caaagcttag atgcatatgt aaaaaccagt
     9541 cagcggatta ttgggaatcc atcaagcttg catgatttag gcaatcaagc taaccggttg
     9601 ttgcagcaag caagaaagca gatcgctgat ttgatccatg tagaaccaga agaaatctat
     9661 tttacttcag gcggaacaga aggagataac tgggtattaa aaggaacgat gatcgaaaaa
     9721 agagaatatg ggaatcatat gatcatttca ggagtcgaac atccggccgt cagcgagaca
     9781 gcagaacaat taaaagaatt aggaatcgaa gtaagtatcg ccccagtgga taaacgcgga
     9841 tttgtacgtg tcgacgaatt gaaagaatta ataagaaaag agacagtatt agtctctgtt
     9901 atggctgtga ataatgaagt aggatcgatt cagcccatcc aggaaatcag tgatcttttg
     9961 gatgattttc ctaaaattca tttccatgta gatgctgttc aggcgattgg aaaagtacca
    10021 ttctcgcaat ggttgacaga aagagttgat tttgcaacat tttcagctca taaattccat
    10081 ggcccacggg gaactggttt catttattgg aaaaagggtc gtcgtttagc accattactg
    10141 acaggtggag ggcaagaaag aaatcagcga agtgggacag aaaatgttcc aggtattgtg
    10201 gctatggtta aagctttacg tttatcatta gagataaagg aacaaaaacc tgagcagaca
    10261 caacgcttaa aagtagctct aatcaatgcg ttgaagacgt atgagaaagt gacgattttt
    10321 tcagaaggaa ctgactatgc accgcatatt ttatgttttg ctctaaaagg aattcgcggc
    10381 gaagttctcg tccatgcctt agaagaaaaa caaatatttg tttctactac tagtgcctgc
    10441 tcctcacgta aacatatggc aagcagtaca ttgcacgcaa tgcatgtacc aaatgatctg
    10501 gctacatctg ctgttcgaat cagcttagat gaaaacaatt cgcttgcaga agtcgagcaa
    10561 tttatgatta ttttcaagca gctttataag aaattttctg caatcaatta aaagaggagt
    10621 gttcttgtga aatacagtga aattatggtc cgttatgggg aactttcgac aaaaggaaag
    10681 aatcgaaaaa gctttatcat gcaactagca caaaatgtca gacaagcttt gaaagacttt
    10741 ccagaaatca aaatccatgc tgatcgggac cggatgcatc ttttattgaa tggcgcagac
    10801 gggcagctag ttattccaaa actagcaaaa atatttggga tccaaaattt ctctccaagt
    10861 attcgtgtag aaaaagatgt cgaagttttg aaacaagctg ttcaagacat catgaaagaa
    10921 atttatactg ggaaagaaac atttaaaatt atcgctaaaa gaagcgatca ccaatttgaa
    10981 ttgacaagta atgaattgaa tcaaacatta ggtaatgctg tttttgatat tttcccgcat
    11041 atccaagttc aaatgcgtca accagatatt ccattacgtg tagagattcg gcgtgatggc
    11101 gcttatctat cgtatgaaac gatcaaggga gctggaggat tgcccgtagg aacaagcgga
    11161 aaaggaatgc ttatgctttc tggggggatt gattctcctg tagcagggta ctttgccatg
    11221 aagcggggag tggaaattga agctgttcat tttgctagtc cgccgtatac aagtgaacaa
    11281 gcactgcaaa aagcaaaaga tctaacagcc aaactagcac cttatgtggg aacgatccat
    11341 tttatcgaag tgccatttac cgagattcaa gaagaaatca aaaagtcagt gccacaagga
    11401 tattggatga caattactcg tcgaatgatg ttgcgtttga ccgatgaaat tcgccgtatt
    11461 cgtcatggtt tagtcatttt gaatggtgaa tcattaggtc aagtggcttc acaaactttg
    11521 caaagtatgg tggctatcaa tgaagtaacg aatacgccaa tcattcggcc tgtagcgaca
    11581 atggataaac ttgaaatcat tgaagtagca gaacagatcg atacatttaa tctttctatc
    11641 cagccttttg aagattgctg tacgattttt gctcctccac agccaaaaac acgccctcaa
    11701 ctggaaaagg tccatcaata tgaagagcgt ttagcaatag atgaaatgat ccaacgagca
    11761 ctagctggct tgaagatgga agagatacaa gcagaatcgt ctaatgaaaa aactgaagaa
    11821 ttttcagaat tgctataaga ggttatgaaa atagtgtttt gaccagagta ttagagaaac
    11881 aaaacgaaaa aatagctcca tgtattttac cgtttttgaa ctaatgtcga agattagctg
    11941 attgaatacc atttatcaag aagttatgga aaagccggtt cttttttact aactgaaaaa
    12001 taaaatttta tttagagatc aagctacagt tgataaatga tataaaaaag agaatttccc
    12061 atgaaatagc gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttacga
    12121 aaattgcttt tcaaattttt gtgaaatttc ggcttatttt tcgaagaagt tacttctgga
    12181 acaccgtgta tcagaggttg
//
LOCUS       DAZKOW010000090        12167 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000090 DAZKOW010000000
VERSION     DAZKOW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.090"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(32..310)
                     /locus_tag="ACG912_002271"
     CDS             complement(32..310)
                     /locus_tag="ACG912_002271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513566.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(425..1042)
                     /locus_tag="ACG912_002272"
     CDS             complement(425..1042)
                     /locus_tag="ACG912_002272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513567.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1437..2261)
                     /locus_tag="ACG912_002273"
     CDS             complement(1437..2261)
                     /locus_tag="ACG912_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0513568.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2604..3020
                     /locus_tag="ACG912_002274"
     CDS             2604..3020
                     /locus_tag="ACG912_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0513569.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="ACG912_002275"
     CDS             3017..3487
                     /locus_tag="ACG912_002275"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0513570.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="ACG912_002276"
     CDS             3501..4274
                     /locus_tag="ACG912_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0513571.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="ACG912_002277"
     CDS             4288..5100
                     /locus_tag="ACG912_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0513572.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="ACG912_002278"
     CDS             5154..6119
                     /locus_tag="ACG912_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0513573.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6255..8978
                     /locus_tag="ACG912_002279"
     CDS             6255..8978
                     /locus_tag="ACG912_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0513574.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8991..9623
                     /locus_tag="ACG912_002280"
     CDS             8991..9623
                     /locus_tag="ACG912_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0513575.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9831..10055
                     /locus_tag="ACG912_002281"
     CDS             9831..10055
                     /locus_tag="ACG912_002281"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFQ0513576.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10157..10390
                     /gene="relB"
                     /locus_tag="ACG912_002282"
     CDS             10157..10390
                     /gene="relB"
                     /locus_tag="ACG912_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFQ0513577.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10380..10649
                     /locus_tag="ACG912_002283"
     CDS             10380..10649
                     /locus_tag="ACG912_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0513578.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10828..12129)
                     /locus_tag="ACG912_002284"
     CDS             complement(10828..12129)
                     /locus_tag="ACG912_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0513579.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         3973 a         1973 c         2387 g         3834 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcagatgcag cgattaaatg actatcttta tcaactaaca ttgcttttga
     6301 aatctacgcc ttcagataat tattcagctc gcagtatttc gcaagctcta cagttgaata
     6361 gaagtacaat cagtagctac ttaaatgaag gtgttcgtga agggcttttc ataaaagtaa
     6421 aaagttaccc ggttttattt ttacatagaa cagctcttga agaactacat atcacactga
     6481 ataactccga aatagaatca attgaaagct tgttaacaat ttctcaaaaa cccgcattag
     6541 atcaagtaat tggctctaaa ggaagtttga aagaagcaat tgatcaaatt aaaacggcag
     6601 tcttatatcc tggaaaaggg ttgcctttat tattgatcgg cgctagtgga tcagggaaaa
     6661 cctttctagc gaataaaata tatgaatatg cagtagaaga aaaggtcatt cacacatcgg
     6721 cacctttcat agattataat tgtgcacaat atgtgagcaa tccagagtta ctatcctctg
     6781 ctttatttgg ctacacgaaa ggagcattta ccggagcaag ccaggagcat actggtttgc
     6841 tagaaaaagc cgatggagga gtattgtttt tagatgaagt gcatcgctta tctgaagaag
     6901 ggcaagagaa attatttaca tttatggata cgggtgaatt ctctcctatg ggggataata
     6961 gtattcggaa aaaagccgat gttcgattag tctttgcaac aacagaaaat atatatacga
     7021 ctttcttacc aacttttctt aggagattgc cagttattgt taatctacct agatttcaac
     7081 aacgtccgtc gtttgaacgt ctgtcattga ttgatgaatt ttttgtttct gagagccaaa
     7141 ttttagctaa ggaactatct gtttctgatg cattgattca tttcttaatg aatagtgatt
     7201 ttgaaggtaa cgtggggaat attaaaaata ttatcaagta tgcttgcgga aacgcatata
     7261 tccaccagaa gagtcagcaa acaattcaag ttaaattatt ggatatgcct ttagagtaca
     7321 gttataaatt caaggagcaa ctaaataaac ccagaaagaa aagagcagat cgaatctact
     7381 atccagaaga aacacgacaa gtgcaactag aaagaaacaa tcagcttttt gttcaatttt
     7441 ttgaccaatt acttactgga ttctctgaag tgattgaaaa taacaaggat attaaactat
     7501 ttttagaaga aatggttacc aaggtaacac aagtaatgga tacgcttatt tttcaagagg
     7561 attacgaaaa agagcagtct ttatacgcca ttctaaacta tcatattcgt caaagtattg
     7621 attttatgcg agaaagctac gggtttgaac aagatggtaa ccgggtagtc gctttggcca
     7681 actatctata tgtcaaggat aatcaagtga tttttgaaaa tagtcttcaa gaaaagaaaa
     7741 ccaaagatca gcttaacgaa ttttttgcta atgttcttga aaccccttat tggtatgcaa
     7801 aaaaaatctt aagcctttta tctgagtgtt tagatcaacc tgtgtatgaa gaggacattt
     7861 tctttattac gctatatttt tatagtttga atgtcacgaa tgttaaaaca ggtattaaga
     7921 gtattgtgct agctcatggt tattctacag ctagtagtat ggcgaatgtt gccaaccgta
     7981 tgctgaataa gaacgttttt caagcttatg atatgccttt agatattaca ttagagaaaa
     8041 ttgaagcaaa attgattcaa ttccttggag attatcgaac agattcaggc ttgattttat
     8101 tggtagatat gggttcacta aaccaactag gcggtttatt agtgaatcat ctaaatggac
     8161 cactgttaat cattgatcat atcaatacac cgttattatt ggaagtcggc caacaagtat
     8221 taaacaataa aagtatcacc gaaataggag cagaaattga aacaagtctt actttcaaaa
     8281 agcagttact acttcccaag aaaaagaaaa agaaagccat cattacttgt tgttatacag
     8341 ggattggtag tgcgacccag attcaggaga ttttaacgaa atgcttgaaa gatgcagtta
     8401 gagaattagt aattattccg tatgattaca aaaagctgtt agaaaacaag aattttgaga
     8461 ctccttttca attatacgat gtcatcatga ttgtaggtac agaagatccc aaaattaatc
     8521 agattccata tattggatta gatcgcctga ttaatggggg aagcattggc gaatttgttc
     8581 attacttgga aaaagagctg actattgaag tgaacgattt acaaaaagaa ttaattttca
     8641 atttttcaat tagtaaaatt gtcgaaaatt taaccatttt ggatccagat aaggtgctat
     8701 cttttgtaca aaaagcaatt aaagaattat cgaagttatt aaattttgat ttttctaata
     8761 atcagctgtt tttgttgtac cttcactgta gttgtatgat tgaacgaatt gtacgtaaag
     8821 aaagtgtgga tgagcaagag gacattgaag aatatatgca agcaaatgga gaaataatgg
     8881 ataaaattca tagcgctttt tttgagattg aaagggaata cacaattgaa ataccagatt
     8941 tagagttgcg attattgagc gatattgttc aaggatagga gtgaattcga atgacaatgg
     9001 gagtaatttt tgatatggat ggggtactca tagatagcga gacattttat tttgagagaa
     9061 gaatgaactt ctttcaagaa ctggagatag atccaggatc aaaccagatt gatgattatg
     9121 tggggaaaac agaagctgca atatgggaaa cattagttcc tgaggacaaa tcattaagag
     9181 aggaacttta tccgaaatat gtagattatc gaaaaacgca tccaattgat ttcgctaaag
     9241 ctttacgaaa agaagtgaaa ccgttacttg acttcctcaa gaagcgggat ataccattgg
     9301 cactggcatc ttcttctcca gctgtcgaga tagagagtat gctcattcaa tgtgaactta
     9361 ctgaatattt ttcctttgtt ataagtggtg agtcattaaa acaaagcaaa ccacatcccg
     9421 aaatatattt aaaagcgaaa cagcaattag actgtgactt ctttattgca gtagaggatt
     9481 caacgattgg tattaaatca gcaaaggcag caaatatcta tacggcggca ctaaaacaac
     9541 caatgcaaat cgatcagagt gaagcagaca tacaaattga aagtctacaa gaactggaac
     9601 aaattgtttt agaaaaaggt tagtgaatta atggaaataa ggctatatat ctgtgctagc
     9661 tattttgact aaccaaaaaa acttgctata attcaggtgt agtaaaaggt gctggtaaca
     9721 ccttttacga aaatgcttcg gcagtgataa tgggattggc tttttaagtt ttttattttg
     9781 ttcgcaatcc ctaaggtcat gtgaatgatc ctagggattg ctagaatatt ttgcaacaga
     9841 accaaaatac gaatagttat caagcagcca tattgactgc ggtcaattta ggaggggata
     9901 ctgatactac tgctgccgta actggtgctc ttgcgggtat tctttacggg ggtgaagtga
     9961 tccctgaaaa ttggagaaaa tcgcttcaaa atgaccagct tcttaaaacg atcagtcaga
    10021 gattttgcga taccgtgcta tctcaaagaa tttgaataat ggatatgatg caaatcttgt
    10081 ttgtactaac gttgtattga caaaaaggat tttatttagt acaatgttag tacaaatgat
    10141 gaacggaggt agaatcatgt ctcaatcaac cattacattt aggattcccg acgatgaaaa
    10201 agaactggta ttagaatatg caaaagcaca caattcttca ttgaccgaac tttatcgaaa
    10261 tgctgtactg gataaaatcg aagatgaaat cgatctacgg actttacgga gtgcaattga
    10321 agtttccaaa gagaaaaaag agagtggtat ttctcaagag gaaatggaga aactgctaaa
    10381 tgaattataa agtcatctac atgccgcaag cccagaagca attaaaaaaa atggacagaa
    10441 accaagctaa aataattgtc gcctggatca gaaagaattt agaaaacacg actgacccgc
    10501 gtcagcacgg aaaaggtttg actggaaata agagtgggga atggcgctat cgaattggaa
    10561 actatcgaat attggctaat atttgtgatt ctgaaattga aatccagata ttctcaattg
    10621 gacatagaag tacaatatat aaaagataac cgtttttgga acgaagtaga gtttcgaaag
    10681 tcaatgtata atcttttgaa ttttgcataa taccacaggt agaaactacc cgatactatc
    10741 ggttggtttc tgctttttta tggctctttg tcaaatagga ctgatagagt gagataagaa
    10801 catctaaggt agaattcctt ttctacttta gatgtttttt gattcatagg taatcagatt
    10861 atttagtaag aaaattctgg tcttcatttt ccgaaagctt cgaaagccat aagcgactcg
    10921 tttcaatgct ttgatgtggg tatgcagatt ttctattttg gcatttgaat aaggcaattc
    10981 gagggctaaa cgaatgccgg attcatagtt tataaaagct ttttttatag aatgcagttc
    11041 ttttggaacg gaattgggca tcgttcttac aagtttaaag aaagtgtccg cctctttgtc
    11101 gtgaaacgct tgaagaattt cttgatagta aaaataggcg attcgtaatt cgtcagagat
    11161 cgaaagcaga cgatccacca cttcactttc ggtgagatac gcccaaccaa aattgcgcca
    11221 tttggtccat ttgtcataaa caagcttgtc ctgtcgtttg atcagaagcc gagacaacgc
    11281 ctttaattgt cgataatatt tggcttgtgt tggatcgttt cgatcaaagc ttttcatgat
    11341 tcgtacgcga atatcctcaa atttacgaat gagatgtttg acaatgtgaa aacgatccac
    11401 aacgagtttt gcctttggaa aacattcctt tatcagagag gcataagaaa cattcatgtc
    11461 cgtcacgatg acctttacct tcaatcgtgc gcgtcgttca aattgcataa aataagaaac
    11521 gagagccttt ttcttccgtg aaggaagaat atccagtaat tttccagtat cgccatccat
    11581 acaagaaaag gagtagggac cgacttgatt tttcagtgaa cgaaattcat ccattaataa
    11641 acaacgcggt aaccaatttc tttttacttt tgtcatgggg atcagagaag cgagtgtccg
    11701 agaaacggtc gaccaagaaa cgccgcaatg cttagcaatt gtcgtcattg cttgtttttc
    11761 agttagctct tctaaaattt tatagcgcac ttgtttagcg ataaagcagt ttctatcgac
    11821 aatgtaggta ttcgccgtaa aataggtgtg gcagccacga cagtggtagc gttgtttcgc
    11881 taaagcaaga taagtcagtt ggttcgcaca tttattaagt tgaatgagcg attttttctt
    11941 tccgttctta acgatcatgc cttcatgatt catctgttta cacaaaggac agcgacgaat
    12001 ttctttactt aacgtcgcag aaacaacaag actcttgata ttcttaatgc tcttcgattc
    12061 cacatttgtg atgaaaatgt ttttatcttt gattccaagt agtagtttga tatgatgaag
    12121 atcaggcata cagaacgctc ctttgattgt gttgtggtga ttcaatt
//
LOCUS       DAZKOW010000091        11979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000091 DAZKOW010000000
VERSION     DAZKOW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.091"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            55..297
                     /gene="yaaA"
                     /locus_tag="ACG912_002285"
     CDS             55..297
                     /gene="yaaA"
                     /locus_tag="ACG912_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFQ0513580.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            284..1408
                     /gene="recF"
                     /locus_tag="ACG912_002286"
     CDS             284..1408
                     /gene="recF"
                     /locus_tag="ACG912_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFQ0513581.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1405..3351
                     /gene="gyrB"
                     /locus_tag="ACG912_002287"
     CDS             1405..3351
                     /gene="gyrB"
                     /locus_tag="ACG912_002287"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFQ0513582.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3371..5842
                     /gene="gyrA"
                     /locus_tag="ACG912_002288"
     CDS             3371..5842
                     /gene="gyrA"
                     /locus_tag="ACG912_002288"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFQ0513583.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6078..6377
                     /gene="rpsF"
                     /locus_tag="ACG912_002289"
     CDS             6078..6377
                     /gene="rpsF"
                     /locus_tag="ACG912_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFQ0513584.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6427..6939
                     /gene="ssb"
                     /locus_tag="ACG912_002290"
     CDS             6427..6939
                     /gene="ssb"
                     /locus_tag="ACG912_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFQ0513585.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            6965..7201
                     /gene="rpsR"
                     /locus_tag="ACG912_002291"
     CDS             6965..7201
                     /gene="rpsR"
                     /locus_tag="ACG912_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFQ0513586.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7344..9317
                     /locus_tag="ACG912_002292"
     CDS             7344..9317
                     /locus_tag="ACG912_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFQ0513587.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9323..9775
                     /gene="rplI"
                     /locus_tag="ACG912_002293"
     CDS             9323..9775
                     /gene="rplI"
                     /locus_tag="ACG912_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFQ0513588.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10049..11416
                     /gene="dnaB"
                     /locus_tag="ACG912_002294"
     CDS             10049..11416
                     /gene="dnaB"
                     /locus_tag="ACG912_002294"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFQ0513589.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         4006 a         2179 c         2651 g         3143 t
ORIGIN      
        1 gaaaaaaaag gtataataga gtatactttt aataatgaaa atgagggatg ggcattgaaa
       61 caacaagtca ttctaaatac ggaattcatg acgttgggcc aaatgttgaa agaagtcaca
      121 gttatcggta gtggcggaca agcaaaatgg tatttggcag aaaacactgt gttagtcgat
      181 ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg gcatgatggt tgaagttcca
      241 gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg ataatgaggc tgaataagct
      301 gtatttaaaa aattatcgaa attacgaaga gctgaacatc gaattttcca agaacctcgt
      361 catcttttta ggagaaaatg cgcaaggaaa aacgaatctt ttggaaagta tctacgtatt
      421 agctatgacg cgaagtcatc ggacaagtag tgagcaggaa ttgattggct ggactgacga
      481 tcaggcgatg attcaaggag agatcacaaa aggttcatcc actcttccat tagaaatctt
      541 attatctaaa aaaggccgaa aaaccaagat caaccatatc gaacagaaaa agttaagtac
      601 ttacgtggga caaatgaatg tcattttatt tgcaccagaa gatctttctt tagtaaaagg
      661 cagcccgcag atcaggcgga agtttttaga tatggaatta ggccagatca atccaatcta
      721 tctttatgat cttgtccaat accaagcaat tttgaaacaa agaaaccagt acttgaagca
      781 attgaatgaa aaaaaacaaa ctgatttgct ttatttggat attttgtccg aacaactagc
      841 agcgtttggc agcaaagtat tgaaagcaag agcacagttc gtcaagcgat tggagttttg
      901 ggctaattct ttgcatcagc aaatcaccca tcaaaaagag cagctggaaa tcgagtatct
      961 caccgcagtc gattctttag aaacacatac gcaagagcaa atccaagaac aatttctcgc
     1021 acttttgaac caaaataaaa agaaagacct ttttcgggga acaacgacag ttggtcctca
     1081 tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg cagacttatg gatcgcaagg
     1141 acagcaaaga acaactgcac taagtgtcaa attagcagaa atcgatttga ttaaagaaga
     1201 gacaggggaa tatccaatcc ttttgttaga tgatgtgatg agcgagctgg atgataatcg
     1261 tcaactccat ctgctagaaa caatcgaagg aaaagttcaa acgttcttaa caacaacgac
     1321 acttgaccat gtaaaaaata aaatgactgt tgaacctgag attttttatg ttcaacaagg
     1381 gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa gtttagtaga acgcgctaaa
     1441 gagtatgatg ccagtcagat tcaagtactt gaaggacttg aagccgttcg taaacgacct
     1501 ggcatgtata tcggttcgac tagttctgag ggtctgcatc accttgtttg ggaaattgtc
     1561 gataattcga tcgatgaagt attagcaggt tttgcaacga aaatccatgt gattattgaa
     1621 aaagacaaca gtatcaccgt tattgacgac ggtcggggga tacctgtaga tatccaggca
     1681 aaaactggac gtcctgcagt tgaaacggtc tttactgttt tacatgctgg aggaaaattc
     1741 ggcggtggtg ggtacaaagt ttctggtgga cttcacggag taggatcttc tgttgtaaac
     1801 gcgctttcta cacagcttga tgtcaaagta tacaaagaag gaaatgtgta ctatcaagaa
     1861 tatcgtcgtg gagctgtagt ggacgactta aagattatcg aacaaacgga tcgccatgga
     1921 acaacggttc actttacgcc agaccctgaa atttttacag agacaactga gtttgatttt
     1981 tcaaaattgg ctacccggat ccgtgaactt gctttcttga accgcgggat gcgtatatcg
     2041 attgaagaca aacgagaaga agaaccagtc atcaatgaat accattatga cggcgggatc
     2101 aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga tttttccaga acctgtatat
     2161 ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta tgcaatatac agacggctac
     2221 cattccaata tcatgagttt tgccaacaat atccatacgt atgaaggtgg aacacatgaa
     2281 tcaggattca agactgcttt aactcgtgtg ataaatgatt atgcccgaaa acaaaagctg
     2341 atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc gagaaggatt gacagctgtt
     2401 atttctatca agcatcctga ccctcaattc gaaggacaaa caaaaacaaa attgggcaat
     2461 tccgaagtac ggacagtcac tgatcggtta tttagcgaac atttcatgaa attcttgctg
     2521 gaaaacccaa gtgtgggccg gcaaattgtt gaaaaaggat tactagcttc cagagctcgt
     2581 ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag gtgctttaga gattagtaat
     2641 ctgccaggaa aattagcgga ctgctctagc aacgatcctg aaaaatgcga attgttcatc
     2701 gtcgaaggag attcggccgg cggatcagct aagcaaggtc gtaatcgaga gtttcaagcc
     2761 atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag caagcatgga caagatcttg
     2821 gcaaatgaag agatccggtc tttatttact gctatgggta cagggtttgg agaagatttt
     2881 gatgtatcga aagctcgcta ccacaaatta gttatcatga ctgatgccga tgtcgacggc
     2941 gcgcatatcc gtacattgct gctgactctt ttttatcgtt acatgcgtcc tattgttgaa
     3001 gctggatacg tctatattgc tcagccacct ttatacgggg taaaacaagg gaagaatatt
     3061 acgtatgtac aaccaggaaa aaatgcagaa gaagagttga aacaagtcgt tgcttcactt
     3121 cctgcctcac caaaaccaag cgttcagcgt tataaaggac ttggagaaat ggatgatcat
     3181 caactatggg aaacaactat ggacccaagc aatcggatga tggcccgcgt cagtgtggac
     3241 gatgctattg cagcagatca gatatttgaa atgttgatgg gggatcgagt agaaccccgt
     3301 cgtgcattca tcgaggaaaa tgcacactat gtgaagaact tagatattta atcatgaagg
     3361 aggaaaaaga atgagtgaag aaatcagaga aaacatccat gatgtcaatc taaccagcga
     3421 aatgaaagac tccttcatcg attatgcgat gagtgttatt gtcgcacgtg cacttcctga
     3481 tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta tacggcatga acgaattagg
     3541 tgtaacaccg gataaagccc ataaaaaatc tgccagaatc gtcggagatg tcatgggtaa
     3601 ataccatcct catggagaca ttgcgatcta tgagtcaatg gtacggatgg cacagccatt
     3661 cagctaccgt tatatgctag tggatggtca tggaaacttt ggttctgttg acggtgacgg
     3721 tgccgccgct atgcgttata ccgaagcacg gatgagcaaa atcgctacag aaatgctgcg
     3781 tgatatcaat aagaacactg ttgatttcca aagcaactat gatgatacag aaaaagaacc
     3841 agttgtacta cctgctcgct tcccgaatct tttagtgaac ggaacaacgg ggatcgccgt
     3901 aggtatggcc acgaatatcc cacctcataa cttgtctgag gtagtagcgg ctatcgattt
     3961 gctgatggat aatcctgatg tgacgaccaa tgaattgatg gaagtattac ctggaccaga
     4021 ttttccaaca ggtggtttgg ttatggggaa atcaggaatc cgaagagcgt atgaaacagg
     4081 taagggttct attaccgtac gtgcaaaagt tgagattact gagatgccaa atggtaaaga
     4141 acggatcatc gtgacagaat tgccttatat ggtcaataaa gcaaaattga tcgaacggat
     4201 ttctgaactg cacagagaca agcgtatcga agggatcaca gatttgcggg acgaatcttc
     4261 ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac gccagcgcat ccgttatctt
     4321 gaataactta tataaattga catccttgca aaattcattt ggattcaata tgttggcgat
     4381 cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatc cttgaaaact atatcgaaca
     4441 tcaacgcgaa gtcatcacac gacggacggt ctttgaaaaa gaaaaagcag aagcaagagc
     4501 acacatctta gaaggtttgc gtattgcttt ggatcatatc gatgaaatca ttgcgattat
     4561 ccgcgggtct aaatcagatg acgaggcaaa agcaacaatg atcgaacggt tcgatttatc
     4621 cgatcgccag tctcaagcta ttttagatat gcgtttacgc cgattgaccg gtttggaacg
     4681 ggaaaaaatc gaaaatgaat accaagaact cttaaaactg attgaagatt taactgacat
     4741 tctggcaaga ccagaacgtg ttacagaaat catcaagaat gaattagcag acttgaatca
     4801 acgatttggt gataaacgac gcacagaatt actagtcggt gaggtcttaa gtctggaaga
     4861 tgaagattta atcgaagaag aagaaatcgt tattacattg acaaacaatg gttacatcaa
     4921 acgtttggca aataatgaat tcagagctca acgaaggggt ggacgtggtg tccaaggaat
     4981 gggtatccac aatgacgact tcgtcaaaaa tctggtttct tgttctaccc atgatacatt
     5041 gttattcttt acgaataacg gaaaagtgta tcgggcaaaa ggatacgaaa ttccagaata
     5101 cgggcgtaca gccaaaggaa tcccaatcat caatttacta ggtatcgatt cgagtgaaag
     5161 catccaggca atcattgcgg tgacgggaga agcagaagaa ggccactatc tcttctttac
     5221 gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct tttgctaaca tccgaagtaa
     5281 tggtctgatc gcaattggtt tgaaagaaga cgatgagctc gtcaatgtgc tactgacaga
     5341 tggtcaatct aatatcatta ttggaacaca taacggctac tctgtcactt tcaaagaaga
     5401 agcagtccgc gatatgggac gtacagcttc cggggtcaga gggatacgcc tgcgtgagga
     5461 agattatgtc gttggggcag ccttaatgaa ggaaaatcaa gaagtattag tgatcacaga
     5521 aaaaggttac ggaaaacgga caaaagtatc ggaatatcca gtgaaaggcc gcggaggtaa
     5581 agggatcaaa acggcgaaca tcactgaaaa aaatggtcca ctagctggat tgaccactgt
     5641 ttctggagat gaagacatca tgctgattac agataaaggt gtcattatcc gtttcaacgt
     5701 atcgactgtt tctcaaactg gacgttcaac attaggcgta cgtttgatga agatggaagc
     5761 tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa gctgcagaac aaacagttga
     5821 agaacaagga tcagaagaat aaccatcaag actaagtgtg taagctagca agaataaccg
     5881 attaaaaaat ctggttattc ctgctggctt ttttgattag ttgaatttct cttaaaatat
     5941 tcgaaactgt tcattctgta ttgcttttga ccaaaataga gtgtataatt agagcttgtg
     6001 agtagtctaa ttaattagat aactctcctt gctcttagga acctaagagc cgaaagtcca
     6061 cagggaggtg aatatcaatg gaaaacacga aatatgaaat catgtacatt attcgtccaa
     6121 acattgatga agaagcaaaa actgctttaa tcgaacgttt cgatgcaatc ttgaaagata
     6181 acggagcaga agttatcgaa tcaaaagatt gggaaaaacg ccgtttcgct tatgaaatga
     6241 acggttaccg tgaaggtatc tatcacatcg ttaaagtatc ttctccatca tcagcaaatg
     6301 ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga tatcattcgt cacatgattg
     6361 ttaaagaaga agcttaattg ttgtttcatg tgaaacaata gaaaacgatg agaggagatg
     6421 actattttga ttaataatgt tgtattagtt ggacgtctga cgaaagatcc agatttgcgt
     6481 tacacagcaa gtggtacggc agtcgcaaca tttactttgg ctgtgaatcg taattttaca
     6541 aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg tgatttggcg caaatcagca
     6601 gaaactttag caaactatgc tcgtaaagga acacttttag gtgttactgg acgtatccaa
     6661 actcgttctt atgataatca gcaaggacaa cgtgtatacg tgacagaagt agtagctgac
     6721 aacttccaat tgctggaaag tcgttctgca tctgaaaatc gccaacaaag cggcggattc
     6781 caaagttctg gtcaatcagc tggcggattt ggcggaaaca ataactctaa tcaaacatct
     6841 caatcatcca acggtatgcc tgatttcgac cgtgatactt ctgacccatt cggctcaagt
     6901 tcaacgatcg acatttcaga cgatgattta ccattctaaa cgaatttgat aggagggaaa
     6961 tagaatggca caacaaagaa gaggcggacg taaacgtcgt aaagtagact acattgctgc
     7021 taaccatatt gaatatatcg attataaaga tatcgaatta ctaaaaagat ttatttcaga
     7081 acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc gctaaaaacc aacgtaaaat
     7141 tactgttgca atcaaacgcg ctcgtatcat gggcttgcta cctttcgtta gcgaagacta
     7201 ataaatccta ggaattgaat gactgatcct tttgatcagc tattcaattc ctttttttct
     7261 gtaaaaataa cctctattcg tttagtcgct cgaatgacga aagttatggt aaaatcaaat
     7321 tagtcatata tgggaggaaa attatggaaa ataaaaacaa tcatttatcc ccgaaacctc
     7381 tcttccttat cctgtttata ttaatcgaag tattggtagt cttaatttta ccatttcgtt
     7441 ttgttagcgc ttcggtcatt ctattgatga atttatgggg tgttcgctat tggttgaaaa
     7501 tgaaacgttg gcttatttta aataaacgtg aaaaaatcca gcaggcttcg actgtagctg
     7561 aaaccaatct agcttatgtc tcagaagtca tgcctgtagg agtgattgct tatcagccat
     7621 caagccaaaa aatcgaatgg ctcaatccat tagcattgaa aatcaaagat caaagtggat
     7681 taacagagga agaattgcta gatacttttt ttactgcaaa agaaaaaaag aaaaaccaag
     7741 tctatctagc agataaaaca tttcagtttg atatggatac agtaaagggt gttgtttatt
     7801 ttaatgatat tacccaaaac cagttgttaa aactccatca gacgaacagt cagccggtga
     7861 ttggtatttt atctattgat aactatgcag atgccattga taaattagat gacaaagaga
     7921 tttcttattt gaactctttc gtcactacac tagtttctga ctggatgaat gaataccatg
     7981 tctttttcaa aagaatcaac gcagaacggt ttttcttcgt agcacagatg atcgatctga
     8041 aaaagatgat ggataaaaaa tttgatttgc tggatcgctt tcgagcagaa gctgaaaaac
     8101 agagctcgcc tatcaccctt agtatgggga ttgcttatgg ctcaagaacc ctcgatgaaa
     8161 tcggtggaga agctcagaat aatttagatg ttgctttagt ccgtggaggc gaccaagttg
     8221 tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta tggggggaaa tctgctaaga
     8281 atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac cgctttgcgt cggatttttg
     8341 atgaaacagg cgatgtgttt atcatggggc acaagtttcc ggatatggat gctttaggct
     8401 ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa aaaagcttac atcgttatca
     8461 atgagaacga gatcattcca gatgtagaac ggtgtcttga agaaatccat aagcatcctg
     8521 aattagaaat gcaactaatc tctccagaac gtgccataga gctgaaaaaa gaagaggacc
     8581 tcctcgtgat ggtggattac cataaacctt ctctatcgat ctctcagggc ctatatgagc
     8641 aatttgataa gattgtgatc attgatcacc atcgtcgtgg ggaagagttt ccaagtaaac
     8701 ctctgctgac ttatattgaa tcttcagctt cttcagcttc agagctagtt tctgagctga
     8761 tccaatacca aagcagcaaa acaaatcgct tagatcgttt tgaggccaca ttattgcttg
     8821 cagggatggt tgtagataca aatagtttca atgtccgtac aactgcacga acttttgacg
     8881 ttgccagcta tttaaggtct tgtggagcag acgcatcctt agtacaatat ttattgagct
     8941 cggatttagc gacatacttg gagatcagca atttgattgc tggaagcgac tatgtgactt
     9001 ctgatattgt tgtggcggca gggacagacg aaaaagagta taatagtgtt atagcagcaa
     9061 aaacagcaga tacattgctt tcgatgatca atatcaatgc agctttcgtc attacaaagc
     9121 ggacagacgg cttgattggg atcagtgcca gaagtaccgg cacgatcaac gtccaaatca
     9181 tcatggaatc cttaggtggc ggaggacact tcacaaacgc agctactcaa ctggaaaata
     9241 cgacagttga ggaaacaaaa cagaagctat ttgctgcaat caaagaaaat atgaatcaaa
     9301 tatatgataa ggagtaatcc aaatgaaagt aattttttta caagatgtaa aaggaaaagg
     9361 taaaaaaggg gacatcaaag aagttccagc aggttatgca caaaattatc taatcaaaaa
     9421 taacttggca aaagaagcaa cgaaaagcag tatcagtcag ttagcaggac aaaagaaagc
     9481 acaagaaaaa cacgaagctg aaatcttgga agaagctaaa aagttaaaag aattcttgga
     9541 aaaagaagat actgtagtcg aattgaaggc gaaagcaggg gaagacagcc gtttgtttgg
     9601 ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa caatacaaaa tcaaattaga
     9661 taaacgaaaa atcgaattag aaaatccgat ccgttcacta ggctacacaa acgtacctgt
     9721 taagcttcat catgaggtga cggctacgat caaagttcat gtcgtacaag aataacaaat
     9781 cttgatagcg atgttcagct ggtaagtata accagaagga aaaaagacaa accgaaattt
     9841 atctgagaaa acagctgcag caatgaaagc aaaagctgca aatcgatttc agattgattt
     9901 cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa cgagtataat aaaagagaac
     9961 tattttttta ttacactttc ggtacgaatg atgacttttc tctcattttc ttgtaaaatg
    10021 aagcaattga agacaaggag cgatacgtat gaatgaagtg tggcaggatc gaatcccacc
    10081 gcaaaatatt gaagctgaac aagcggtttt aggctctgtt tttcttgacg cagaaacgat
    10141 cattgatgcc atggagtaca tagagccaaa agacttttat cgtcgtggcc atcaaattat
    10201 ttttgagaca atgatcgagt taaatgatcg taatgaagcg attgacgtgg ttactgtcaa
    10261 agaccggctg gaacaagcca atttgttaga agatgccggt ggtttaagtt atctctcaga
    10321 cttggcatta gcagttccga cagcagcaaa tatcgtctac tatgcgaaaa tcgttgaaga
    10381 aaaatcattg ctgcgtacat tgatccagac agcaacagga atcgttacga aaggattcga
    10441 gcaaggagaa gaagttcaaa caattttaga tgatgccgaa cgcagtatct tggaggtttc
    10501 ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa gtactgaact ccacgattga
    10561 aaatatcgat cgactagctc aaaacaatga agaaatcaca ggtctaccaa ctggctatca
    10621 aggtctggat aaaatgacag caggtttcca gcccgaagaa ctaatcattc ttgctgcacg
    10681 tccagcggta ggtaaaacag cgtttgcatt gaatattgct caaaatgtag gaacaaaaac
    10741 agatcgttct gttgcgatat ttagtttgga aatgggggca gaatctcttg taaatcggat
    10801 gctttgtgca gaaggttcga ttgaggccag ccatttacgt acaggtcagc tttcggaaga
    10861 agaatggtcg aatctgatcg ttgcgatggg gagtctttct cgagcaaata tttttatcga
    10921 tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa tgtcggaaac tagcgcaaga
    10981 aaaaggcaat ctaggcttga tattgatcga ttatttgcag ttgattgaag gtaacggtaa
    11041 agagaaccgt caacaagaag tatcagatat ctctcgccaa ctgaaaaaac tggcgaaaga
    11101 gctgaaagta cctgtcattg ctctttcaca gttatctcgt ggagtagaac agcgacaaga
    11161 caaacgacca gtgctaagtg atattcgtga atccggatca attgaacaag atgcggacat
    11221 cgtagccttc ttgtatcgag atgattatta tgaaagagaa ggcggagatg acgatgatgg
    11281 aaacgagcca aaaaatgaca acgtcatcga agtcatcatc gagaagaacc gaagcggtgc
    11341 acgaggaaca gttgaactac tgtttatcaa agaatacaat aagttttcgt cgctttcgcc
    11401 aagagaagaa ttttaaagaa gaggttgtga gccttgagca atcccgagag ctctcgtaag
    11461 aagatgcaag gagtaaccgc aggaacgact agagtactta atttaaacag atctccaaag
    11521 ggattgtgac aaaagtcttg tcacagtccc tttttccgaa taaacggtgt tcaatcagct
    11581 gacccttggt attagctcag caaacgtcaa aatatgagga actattttga cgtttgtttc
    11641 tctaatactc aggtcaaaac gctgatctta cgacctcttt tccttaaacg gtgttctcct
    11701 gccaactcct cggtcacaag tcacaatatc atcaaacatg agaagcagtt ttttgaaatt
    11761 gctccttgtg actcagagtt aaacggcagg ctcacaacct ctttcttaaa cggtgttcaa
    11821 aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa tatgagaagc tatttttgtt
    11881 tggctgttct tattacttga tgcaggacag ctttttcaca acctctgatt cacggtgttc
    11941 caaaagtagc ttcttcgaaa aataagtcga aatttcaca
//
LOCUS       DAZKOW010000092        11542 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000092 DAZKOW010000000
VERSION     DAZKOW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.092"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(119..1225)
                     /locus_tag="ACG912_002295"
     CDS             complement(119..1225)
                     /locus_tag="ACG912_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFQ0513590.1"
                     /translation="MIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLSWSVIFLAR
                     SVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQFQPSELANL
                     FLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIAWVIFWAAA
                     VPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDSFIDSHGAG
                     YQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIGALCVLFLL
                     FSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGVPLPFVSYG
                     GTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     regulatory      complement(1399..1565)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            complement(1790..1996)
                     /locus_tag="ACG912_002296"
     CDS             complement(1790..1996)
                     /locus_tag="ACG912_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513591.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2012..2626)
                     /locus_tag="ACG912_002297"
     CDS             complement(2012..2626)
                     /locus_tag="ACG912_002297"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFQ0513592.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2773..3456)
                     /locus_tag="ACG912_002298"
     CDS             complement(2773..3456)
                     /locus_tag="ACG912_002298"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0513593.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3565..4023)
                     /locus_tag="ACG912_002299"
     CDS             complement(3565..4023)
                     /locus_tag="ACG912_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFQ0513594.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4107..5870)
                     /locus_tag="ACG912_002300"
     CDS             complement(4107..5870)
                     /locus_tag="ACG912_002300"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFQ0513595.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6091..7551)
                     /locus_tag="ACG912_002301"
     CDS             complement(6091..7551)
                     /locus_tag="ACG912_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513596.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="ACG912_002302"
     CDS             complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="ACG912_002302"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFQ0513597.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8708..10135)
                     /locus_tag="ACG912_002303"
     CDS             complement(8708..10135)
                     /locus_tag="ACG912_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFQ0513598.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10166..11380)
                     /locus_tag="ACG912_002304"
     CDS             complement(10166..11380)
                     /locus_tag="ACG912_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFQ0513599.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3496 a         2396 c         1907 g         3743 t
ORIGIN      
        1 taatatgatt ttttgagata ctttcattgc gaattcctct ttctaaaaga acctctcgct
       61 tgaaaaataa aagattgtac tttatgataa ttcaaactga gagtttctca gtttctcttt
      121 attgtttttc aggtcgatag agcgccaact cttcggcctg tatctttgaa gaaatattca
      181 acgtgattcc gattcctaaa gaaaggatca gataacttgt tcctccgtag ctaacaaatg
      241 gcaatggcac accagtcatt ggcatcagcc ccgcgatact tcccacgttc attatcgtct
      301 gaacgaatag caaagttccg aatcctaaaa gaaacaatcc agcttgctga tttttacagc
      361 gactgcttaa acaaaagatt ctcatgcata atgagaacaa taagaaaagc acacacaatg
      421 caccgattag cccaagctct tctgtaataa tcgaaaaaat aaaatctgtt tctgtctctg
      481 gaagataccc tttttttgtg atactatttc ctaatccccg cccaaatatg ccaccattat
      541 atagtgcata aaacgaatgg gtcatttgat agcccgcacc atgactatcg ataaaagaat
      601 ctcttaaagt cgcgatcctt tcataagcat gattaaacat ctgtggaagc caacctttat
      661 ttcctaaata taataccccg cctgctgctc caatcagcaa tgcagaaaaa gtaacgatta
      721 gatagatccc ttttttaaat ggaaccgccg ctgcccaaaa tatgacccac gcaatcgaaa
      781 ggatcatcaa tgctccagca atctttggct gaaataaaat cagaaaagtt atacctactg
      841 taatcaggaa tggttttttt agatcttttg ggctactatt tccgtcacga aaaaaccagc
      901 ttaaataaaa aatcaaaaaa agatttgcca gttcagaagg ctggaattga atgccaaaca
      961 gagagatcca acgttgtgcg ccgttgacag tgacaccgaa tatccctatt cttactaata
     1021 ctaaaaagaa aatcgataag gctaaaccgt atcctgctat tttagggtga agtaaatagt
     1081 gtagtttgac tgaacgagct aagaagatca cgctccaact caaaaatatg aaaattagtt
     1141 gacgcaataa tagacttttt gtattttcat cggcttgcaa taaacgataa gaactggcac
     1201 tgtatacttc taaaagaccg atcatggaca aagtcaaata aggacccaaa atccaccaat
     1261 cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg tgaggagcga
     1321 gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga aaccttttcg
     1381 aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat acaacgtaaa
     1441 gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt tacccattgg
     1501 cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac ctaacaaggt
     1561 tattactata ttctatttac gagttcatga tagacttttc ctaatgatta ggcaatggaa
     1621 tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa aataacaaat
     1681 aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt gataattttt
     1741 tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt tatttgttaa
     1801 agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta ccatctaaag
     1861 taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac tgcccttgca
     1921 gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc gatggtttat
     1981 agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc gtgaaaattt
     2041 ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat tttcttggat
     2101 cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc tgtacttcta
     2161 gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt tcatataagg
     2221 atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt actgaatcag
     2281 ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg gctgctgttt
     2341 tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct cttgtctcaa
     2401 gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc aaggataaga
     2461 tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc actatatctt
     2521 ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca atcagcaact
     2581 tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt tcgccttctt
     2641 tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat gcttttttca
     2701 tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa gaatctgtgc
     2761 cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt tagtgatcaa
     2821 ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt catatgcttt
     2881 ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat cgacttgttc
     2941 tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat attctgtata
     3001 gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc
     3061 aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt ggacaagttc
     3121 agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa tgatttctgc
     3181 ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca cttctgaccc
     3241 atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct tgatttcatt
     3301 gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg cagccaaagc
     3361 cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt gacaagaaac
     3421 gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag taatgttata
     3481 taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca ataatgagct
     3541 atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt cgtatacgac
     3601 cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt taaaaaactc
     3661 agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt tccaagcatg
     3721 ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga tgatacaaac
     3781 tggttcattt tttccattca ggaccacgtc taattgacca acttttggta aaggctcatt
     3841 ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt ttacaactag
     3901 ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac ctaatttttc
     3961 tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta cttgctgata
     4021 cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact agtataaaca
     4081 attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag actatcttca
     4141 acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg aacgctatcc
     4201 gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc ataataacga
     4261 ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat gactaattcg
     4321 tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt tcctggattg
     4381 atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt tcctaattca
     4441 agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt ttgataggct
     4501 ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt aggaattcgg
     4561 attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat catatcctct
     4621 tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg aatgtcaaat
     4681 gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa aataatcact
     4741 ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg caagaaagta
     4801 cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc gaaagtgata
     4861 gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt
     4921 ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc tgtacttgtt
     4981 tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc agaagttact
     5041 tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt cattttatta
     5101 tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac aatcggatga
     5161 tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt tccattatcc
     5221 acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt accgttggtt
     5281 tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat agacatctca
     5341 ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt caatccttca
     5401 aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt tcctgattct
     5461 ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt agatgtgtgc
     5521 cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct cacttcacgg
     5581 taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc ataagactga
     5641 tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg aaacgcaatc
     5701 gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct gtccttttgc
     5761 aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga taattttttg
     5821 acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat aatacattct
     5881 ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc tattctacta
     5941 cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag cttgttatat
     6001 aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca attaaaaagc
     6061 tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta ataggtgaag
     6121 ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg gatagatctt
     6181 tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa ccactctttt
     6241 tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa taggatttat
     6301 ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa attgtttgac
     6361 tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt cactgttgat
     6421 caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta gttggatttg
     6481 atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa gttgttcttt
     6541 ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc acaacgtacg
     6601 gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga tttcagcgat
     6661 actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa aatttttata
     6721 aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat ttctactgtt
     6781 gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc ctttttgctc
     6841 ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt tcttatcttc
     6901 ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc ctcgtatgat
     6961 aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta cataatcaat
     7021 caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt cccaattgac
     7081 ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa tgatttcttt
     7141 atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga ttgtatatac
     7201 tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata taccattaaa
     7261 ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg tgagctcaga
     7321 aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt
     7381 tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt
     7441 cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc
     7501 acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac
     7561 acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc
     7621 ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca
     7681 aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc
     7741 agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt
     7801 caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc
     7861 tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg
     7921 tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat
     7981 tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat
     8041 tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga
     8101 gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc
     8161 tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat
     8221 ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg
     8281 ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc
     8341 attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt
     8401 gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc
     8461 tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc
     8521 ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg
     8581 tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata
     8641 gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt
     8701 tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa
     8761 taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag
     8821 ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac
     8881 ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa
     8941 gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca
     9001 ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc
     9061 ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag
     9121 caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg
     9181 ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta
     9241 aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta
     9301 ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg
     9361 tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa
     9421 acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg
     9481 tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg
     9541 aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga
     9601 cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac
     9661 cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc
     9721 agaaaataat caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac
     9781 ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta
     9841 tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga
     9901 cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct gctgcgcaca
     9961 atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact aagctgaagc
    10021 ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg gtcatagcca
    10081 aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt tgcatgaatc
    10141 ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca
    10201 atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc gccgatttta
    10261 aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca atcacttgaa
    10321 tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat gcaaagatcc
    10381 ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca tcaagtgcat
    10441 catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata cccgcaatga
    10501 tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta atggctgcca
    10561 atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct tttacttttg
    10621 caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat
    10681 tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac cagccaaacc
    10741 aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt ctttctccta
    10801 aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat ccagctgtcg
    10861 tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa aatccgccac
    10921 cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata acaagtataa
    10981 tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga acggtaatca
    11041 cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca
    11101 tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt ccaataacac
    11161 cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata atcgttgtta
    11221 tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc cgtgctaatc
    11281 cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta cataaaatca
    11341 tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc tcctttctac
    11401 gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg aattaatact
    11461 tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct ttttcgtttt
    11521 tcacacaaat aaagacaaaa aa
//
LOCUS       DAZKOW010000093        11198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000093 DAZKOW010000000
VERSION     DAZKOW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.093"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG912_002305"
     CDS             complement(13..897)
                     /gene="ftsX"
                     /locus_tag="ACG912_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFQ0513600.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG912_002306"
     CDS             complement(890..1576)
                     /gene="ftsE"
                     /locus_tag="ACG912_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFQ0513601.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(1681..2782)
                     /gene="prfB"
                     /locus_tag="ACG912_002307"
     CDS             complement(join(1681..2709,2711..2782))
                     /gene="prfB"
                     /locus_tag="ACG912_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFQ0513602.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG912_002308"
     CDS             complement(2908..5442)
                     /gene="secA"
                     /locus_tag="ACG912_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFQ0513603.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG912_002309"
     CDS             complement(5664..6215)
                     /gene="raiA"
                     /locus_tag="ACG912_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFQ0513604.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(6343..7062)
                     /locus_tag="ACG912_002310"
     CDS             complement(6343..7062)
                     /locus_tag="ACG912_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFQ0513605.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(7059..8372)
                     /locus_tag="ACG912_002311"
     CDS             complement(7059..8372)
                     /locus_tag="ACG912_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFQ0513606.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(8453..8773)
                     /locus_tag="ACG912_002312"
     CDS             complement(8453..8773)
                     /locus_tag="ACG912_002312"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0513607.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(8881..9168)
                     /locus_tag="ACG912_002313"
     CDS             complement(8881..9168)
                     /locus_tag="ACG912_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513608.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            9295..9939
                     /locus_tag="ACG912_002314"
     CDS             9295..9939
                     /locus_tag="ACG912_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFQ0513609.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            complement(9998..10705)
                     /locus_tag="ACG912_002315"
     CDS             complement(9998..10705)
                     /locus_tag="ACG912_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0513610.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(10925..11095)
                     /locus_tag="ACG912_002316"
     CDS             complement(10925..11095)
                     /locus_tag="ACG912_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513611.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         3053 a         2430 c         1957 g         3758 t
ORIGIN      
        1 tatattgtgg tattagaatt tcaaaaaacg tctcatagaa atgatcgagc caagtgaacc
       61 aatgatcaca ccaatgccga ccattaaaag attgattttc caaacgatat tttgtggacg
      121 aatcaatgaa taatgggaac gtaataatga tggattgatc aattggaaaa accatgcata
      181 accaaatgtg atgatcaaaa tcggaagaat cgcaccgatc agaccgatcc agccaccttc
      241 aaggaaaaac ggccagcgaa tatatccatt ttttgcaccg actaaacgca tgatttgaat
      301 ttcacgctgt ctagacaaaa tcgtgatccg aatcgtgttc gaaatgagaa acatcgctac
      361 gaacagcagc aatgccgctg caactaatcc ccatgtccga actgcaccag cgatttggaa
      421 aatccgatct gaggaagctc caccgtaatc agaacgagaa acattcttca aatctgctgc
      481 ttcttctgct acttgttttg tataagaagg atcagtagca cttactacat acacatcata
      541 taacgggtta ttgtcacctt caaacaattt ccaggaatca cccatttcgt tttggatttt
      601 tttcaattcc tgttctttgc ttgaaaattc aactttttta acatgatcta agtcattcaa
      661 ttctttttct agctttttca tctcttcgtc ttttgtaccg atatctacga atacagatac
      721 atcaacgttt ccttctatat cctgagctaa tttagttgca ttcatgataa ctgccatgaa
      781 tacaccgact aaagtcaacg tgatcgttac cgcactgact gaagcaactg tcatccagcc
      841 gttacgtttc aaactcttga tactttctaa taaatggcgg aaaaaggttc taatcatcgt
      901 atccatattc cccttccgct tgatctcgaa taatgcggcc gttttcgata gcaattactc
      961 ggtgccgaat cgtatttacg atcgtactgt tatgtgtagc catcacgaca gtagttcctt
     1021 gggcattgat ccgatctaat aatttcatga tttcccatga gttttctgga tccagatttc
     1081 ctgtcggttc atccgcgata agtactttag gcgtattcac gattgcccga gcaatagaga
     1141 cacgttgctg ttccccaccg gataattcgc taggaaagac tcgaactttg tgtttcaaac
     1201 caacaaggtc cagcacttcc attactcgtt ttttgatatc tcgaggtttt ctgccgataa
     1261 cttgcatagc gtacgcaaca ttttcatata ctgtttttct tggtaataat ttatagtctt
     1321 ggaacaccac gccgatttct ctgcgtagat aaggaacttc gcggtttttg attgctatta
     1381 gatcatgacc tgcgacgttc aaagttcctt ttgaagcttt ttcttctcga tacatcaatt
     1441 tgatgaatgt agatttaccg gctccagacg gaccaaccac atagacaaac tctccttgtt
     1501 cgatgacaat cgagagattg cggatagcgg tcgtaccatt tgagtacttc ttcattacat
     1561 ccttcatttc aatcatggcg ttctctccat atctttttca gtctttagct attatagcat
     1621 ggcaatgttt caacatgctt gcaaaatctt acaattttat ttcaatttaa caatgagttc
     1681 ttattgtaga tgcattttta aatagccgtc aataaataaa tcaaggtcac cgtccataac
     1741 tgcttggaca tttcctgtct ctacgtttgt ccgatggtct tttaccatag aatagggatg
     1801 gaaaacataa gaacggatct gtgaacccca gccgatttct ttttgttctc ctcgaagtgc
     1861 agctgcttct tgttctttct tttccacttc taattgataa agcttagctt ttaacatccc
     1921 catcgcttgc tcacggtttt tcaattgtga gcgttgtgcc tgactagcta ctactgttcc
     1981 tgtaggcaag tgagtgatcc gtacagcaga ttctgtttta ttgatgtgct gaccacctgc
     2041 accacttgct cggtacgtat cgattttcaa atcatctgga ttgatttcta tatcgatcgt
     2101 ttcatcaagc tctggcatca catctaccga acaaaaagaa gtatgacgtc gtttcgctga
     2161 gtcaaaaggc gaaatccgta caaggcgatg aacacctttt tctgatttta gataaccaaa
     2221 agcgttatgt cctttaatca gcagggtcac actcttgatc cctgcttcat cacctgcttg
     2281 ataatctagt gtttctactt gatagccatg ctgctctgcc caacgtgtat acatacgtaa
     2341 aagcatgctg ccccagtctt gggactccgt tccgccagcc cctggatgca attcgacgat
     2401 cgcattgttt cgatcataag gtccgtctaa cagcatagat aattcatacg tcttcatttt
     2461 ttcatctaat gcattgatac gtgtttctgc ttcttcttgc attgctgcat caggttcttc
     2521 ttgcagcatc tccaccatca attcgatttc ttctagttct tcggcaagag aatggaattg
     2581 gtcataggtt tctttattag cgttattttc gctgatcact ttttgtgcgg cttcgctgtc
     2641 atcccaaaaa ccaggttcag ccatttttgc ttctgcttcc gcgatgtctt cttccagctg
     2701 atctaagtca aagagacctc ctgaaacttg tgaccttttc ctgcatttct gtcaggagat
     2761 ttcgaatttc ggatatttcc atgttttttc cttctttcac tatacttttt tcattatatc
     2821 tagtctaaca gaatcagcaa ataacagaaa gaaggtgaga cgaattctta aaaaaagaat
     2881 ttgtctcacc aatcctgctg tcagaaatta gtttgcattt tttccatgac agttcttata
     2941 ttttttcccg cttccacaag gacaaggatc atttcttccg accttgttca catggattgg
     3001 ctgttttttc gcgctagtat tactattttc tttggcatct ccgtcttctg agtgagaagc
     3061 tgttccttga gaaacctgtt cacgctgtac gttttgacgg atctcagctt tcataaagat
     3121 tcgagtgact tcatactcga tggcgcctac catatcttca aacatcttat agccttctgt
     3181 ttgatattcg actaatggat tgttctgtcc ataagcacgt aagccgattg actgacgtaa
     3241 ttgatccatt gcatcaatat ggtctgtcca tttagagtcc acaacacgca agatcacgac
     3301 tttttcaaat tccaacagtt gctctggacc atttagttga gccgctttgg tattaaatac
     3361 ttcttgtgct cgttcgttta gatactcttt gatctcctga gatgtttttc cttcgatatc
     3421 tgcaagagta atactatctt catggacaag agtactgccg gcaaaatcga tgatactttc
     3481 caaattccaa ttttctttct ctaattgtgt atgcgcatct actacacgac tgattgtccg
     3541 tttgaccatg ttcattagtg gcttggataa gcttttttct tccatgatta cttcttgtcg
     3601 ctgtttatag attacttcac gttgttcacg catcacatca tcgtattgta agacgttttt
     3661 acgtgtgtca tagttatttc cttctacacg tttttgtgca gattctactt gtttcgtcag
     3721 catcttgctt tggataacag catcttcctc accgatcttc atccgatcta agaaagcttt
     3781 gattcgttct gaaccgaatc gtttcatcaa atcatcttcc aatgacaagt agaattgtga
     3841 cataccaggg tctccttgac gtcctgcacg tcctcgaagc tggttatcga tacgacgaga
     3901 ttcatgtcgt tctgtcccga taactgctag accaccaagt tctcttaccc caagtcctaa
     3961 cttgatatct gttccccgac cagccatgtt ggtagcaatc gtcacagcac ctttttgacc
     4021 agcattcatg atgatctctg cttctttaaa gtgatttttc gcattcagca cttcatgagg
     4081 aatacgttct tgattcagca tatctgataa taactcagat gtttcaaccg caacagttcc
     4141 gactaacaca ggttgacctt tgcggtagcg atctttgata tcttgcacga cagcgtggaa
     4201 cttgctttgt aatgttgggt aaagtaaatc tggacgatca tcacggataa ccggacgatt
     4261 tgtcggaatc tggtatactt ggatgttata aatttctctg aattcttctt cttctgtttt
     4321 agctgtacca gtcattcccg caagtttctt gtacatacgg aaatagttct ggaaagtgat
     4381 cgtcgccatg gttttcgttt cgtcttcgat ctctacgcct tctttagctt cgattgcttg
     4441 gtgaagtcca tctgaataac ggcgtccatc catgatccga ccagtgaact ggtcaacaat
     4501 cagtactttt ccgtcttgga caacataatc gatatctaaa agcatgatat agttggctct
     4561 caatgcttga tccaagtgat gggtcaatgc agtattttca atgtcgtata agttatccaa
     4621 accgaatgtt tcttctgctt tttcgatccc tgcttcggtt aaactgattg tttttgattg
     4681 tacatcgatt ttataatctt cttcttcttt caaacgtttc acgaagttgt ctgctcttgt
     4741 atataaagct gtggattttt ctgcttgccc tgaaatgatc agcggcgttc tcgcttcatc
     4801 aatcaagatt gagtcaactt catcgacaat ggcataattc agcggacgtt gtaccatttg
     4861 atggcggtaa acgaccatat tgtctctcaa gtagtcaaat cctaattcat tattcgtact
     4921 ataagtgata tcacaattat aagcctcgcg tttttcatcc gaagattttg agttgatatt
     4981 caaccccact gttaatccta agaaattata aagttcgccc atttccgtcg agtcacgggt
     5041 tgctaaatat tcattgacgg taactacgtg taccccttcg ccagtcagag catttagata
     5101 tactggcatc gttgcggtca aggttttacc ttcaccagta cgcatttcag ggatatttcc
     5161 atcgtgaagc acgattccac ccatcaattg tacgtggtat ggatataacc ccaatacacg
     5221 ttttgctgct tctcggacga ctgcaaaagc ttccggaagt aattgatcta atgtttctcc
     5281 tttttggtat ctgcccttga attcagcagt ttttgcctgt aattgttcat cagttaatgc
     5341 tgccatttgg tcagcgtatg cttcaacttt ttttgcaatg ctatctaaac gttttagttc
     5401 tttcttatcg ttttcgataa gtgaacgaat caaattggcc atgcgttgtg taatctcctt
     5461 ttccttctgt gtattatcca gactatgtag actttacttt tcattttatc attagttata
     5521 gataattgaa atatcttaag cgtattaaag taagaacttc ataatttatt catatttcat
     5581 ctaaaagagg aggaaactca aaaaaacttg cggcagctgt cagtagctgc tgcaagtttt
     5641 cgttgttatt taagtaaggc tgatcagtct gtttcaatca gtccgtattt tccatcttta
     5701 cggcggtaaa cgatacttgt accatttgtt tctgcatctt caaaaataaa gaagttatgt
     5761 cccagcatgt tcatttgtaa gactgcttct tcactatcca ttggtttcaa tgaaaggcgt
     5821 ttcgtacgta cgatatctaa ttcagaatca ttttcgtttt cgtcctcttc attgaataaa
     5881 gcagcagcat ctgcagtatt catgcccgtt tcacgggatt tacgattaat ttttgttttg
     5941 aatttgcgga tttggcgttc caatttatca acaaccaaat cgatacttgc gtataaatct
     6001 ggagaagttt cttcggcacg taatacaaga taaggtaatg ggatcgttac ttccactttt
     6061 gctgtttttt ctgtataaac ttttaagttc acatgcgccg ttgcatcggg tgcgtcactg
     6121 aaatagcgtt ctagtttgcc tacttttttc tcaacgtagt cgcgaatcgc ttcagttact
     6181 tcgatatttt cgccgcgtac attatatcta aacataaaaa ttaccccttt cgtctaccac
     6241 aatcaagaag gagaaggacc actctttccc tcttcttaat tctattatat cgaactctag
     6301 gttatttgca aattaaatcg ctatcaaatt tccacatcta attcatctcg ccaaagaaat
     6361 ggttcggatg attttcggct gtgcgacctg caaacaatct gctgcgtgga aaagagtctg
     6421 tcctgttgta tagacatcat caataatcaa tattttttta ttttgtatct tagaaaaggt
     6481 gactgcaagt tcaaaaggtt gaggcatctc caatctttcc tttcgcgtgt tttttgcttg
     6541 agccagcaga tgcttctctc ttttgagtag catctgaaaa ggaattcttg ctgctttcaa
     6601 catctcgctt acttgattga atcctctttc tgttcgcctt ttttcagata aaggtattgg
     6661 acaatagatg tatgtcgggt attctttttt tatctgcgtc catttatatg caaaggtgcc
     6721 tgctagtcgg atatctccca taaacttgta ttgattgagc caggccttca tattttcatc
     6781 gtacacaaaa aaggcacggt gctgaaaatc ataggtcgga tatttcttct tccattcttg
     6841 acagtcactg cagtaaacgg gaatagactt tactttcttt ttcgtactct ctagattgaa
     6901 atggattggt ttttgacagc cagaacagcc tcttgtttcc aataaatgaa attgtgcgcg
     6961 acaaactgaa caagtctgag gtaatctgta tttcgaaaca ccaataatct cgctgaatgt
     7021 taaattttgg aagatatctt tactgcagca actgcacctc acagcatctt cttcccttct
     7081 tcattcattt ctttgatttc ctgacaagct tttcgaatag ctgcagtttt ttcttcatag
     7141 aaaaagtata cttgactgtt cgtataaggc cctttacgat cggctcttcc tgcgatttgt
     7201 accaaagaag atttagtaaa aaccggatga tccgctccta aaacgataac ggatattctt
     7261 tcaaatgtga ctccacgttc aaggatcatc gtggttaaca gtatatcata ttttgcttct
     7321 cgcattttct ggactttttc cgcccgctga ttgtctcttg aagaaacatc tgtgatctca
     7381 tatctcggaa gagcctcttg aagtgtggct ttcaaacgat gcatcatcgt gatacttgga
     7441 caaaataaaa ggatatggtt ttctttaatt aattgatgaa tgatccgcac caaagatttt
     7501 atcttcttct tgttcttgca tttttgtcgc caattattca tgaaaaccaa gacgggttca
     7561 ggaagcaaac gccgatgaaa gcgtaaagca agtttgtgga tgtcaaacgt ctgcttgatt
     7621 tcctttagta actttttatc aggtgtggca cttaagtaga tgaatcgtcc gtctggttta
     7681 atcgctgttt gcacagcatg agctaatcct gcatccccgt tataaggaaa cgcatcgatc
     7741 tcatcgatca ctattaaatc aaaggaccga taaaaacggt atagctgatg aatcgtacag
     7801 ataacaaaag aagtaaaggt ataagccgtt gctgtattcc catggagcaa cgtgattttt
     7861 tcttctggaa aaacataacg atagcgtaaa aacaattcat tacaaacatc gactcgcggt
     7921 gtacaaatcg ctactcttcc tccagctttt aaagtttcca ctaaaatagg ataaagcatc
     7981 tctgttttcc ctgcacctgt tacagcccaa ataagatgat gtcttttttc tgaatggcaa
     8041 agtattctct ttgagatttc cgtttgtttt tcagtaagct gtcctgtcca aaaaaagacg
     8101 acttctcgag gttttgcttc aggctgatca aacaaatata aatcttgttg agtgtcgcat
     8161 cgccctaaat atagacaatg tgtacaaaaa taaccgtaat ctgcaagctg tccatcaatg
     8221 acggacgtcc tcattccaca gcgatgacaa agaatccagt tgcccaaacg ttccactgct
     8281 gcgatcttct cataatttgg taaatttacc tcctcacagt ctattgcttc aatatctctc
     8341 gtcagaagcc ttctacccca aaaatgattc atattcttcg ccctaaaaaa atatacgaaa
     8401 aaacaagcga tctatttttt ggatcgcttg ttcgatagtt atactgttct atttattcac
     8461 ctatgagcgt gattgcttga tctaacactt tttcgccatt catcatacca taatcagcca
     8521 tattgatgac atctaaagga atgccttttg gttccagacg tttctcaaaa tcagctttca
     8581 taaagcgaac ttgtgggcct aataacaaca catccacgtc tttatttgca aggttgttat
     8641 ctgcgtcaga agcggacaca gcgaaaatat ccgagtcgat tccttttgct tcagcagctt
     8701 tttgcatttt tgttactagt aaacttgtgc tcataccagc cgaacataca agcatgattg
     8761 tttttttagc catacttttc tctccttttt aaaacaaact atgcaaacgt tttcttcgcg
     8821 tctgttactc tattatataa aaagaaagaa atttgtcaac gttttctaaa cgcggtttat
     8881 ttaatcaaat agccaactag atttcactaa tttattgaac acgacatatt ctttgtccaa
     8941 attgatgaca actacttcat agcctgcatt tatccagcca taggcacctt tttcagggtg
     9001 tttataatca tttgcccacc aatctgtact ttcccttgct gttttgggaa gtccgttact
     9061 gggaaacagc agttcgtcaa attgggtaaa ggtcaacgtt acttgagagc cgccattgtt
     9121 cttcaaataa tgcgtaattg catcgtactt tttcattcgt tcctgcatcc ccagatccac
     9181 ctgcctttgt ctttattatc gcatactaca gcaattgcgc aaccgttaca gtgttgaaac
     9241 aacctagaac tttatttata ctgaataaag aaaatgaaaa aaagaggtga caagatgatc
     9301 gagcgctatc gtacaataaa agaaaatgga caaagtgaga ttgagatcaa aaaatcacgc
     9361 tttatctgtt ctcttagacg aatagaatca gaggaccaag caaaaagttt cattcaagaa
     9421 ataaaaaaag aacattggaa agcgaaccat aactgcagcg ccttcatcat tggagaaaaa
     9481 aatgaaatcc agcgaagcag cgatgatgga gaaccaagtg gaacggcggg cgtaccgatg
     9541 ttggaagtcc tcaaaaaaaa tcaactgatc aatgtagctg cagtagtcac tcgatatttt
     9601 ggaggaacga aactcggcac aggcggatta atacgagcat acactcatgc ggtctctcaa
     9661 gcattgtctg acattggaat cgtagaaggt accttgcaac aggagatctc tgtgatgatc
     9721 acgtatccgc aactaggaaa attacagaat tatcttgaac tccaacattt ttttataaaa
     9781 gaaatccaat atactgatca agttagcgtc atctgtgctg tagatgaaaa aagtgctgac
     9841 cactttaaag aatccatcat tgagctgctg aacggacaag tccaatttga agagggttct
     9901 gtttcttatc acgagcaact gataactgat catatataaa aacaggtgat tctgattctc
     9961 ttttatttct aataaggaat cagggatcac ctgtttttta tttttccaaa taaaactcaa
    10021 aacgatttcc tacgtattgc gtacgcacat actcaaacgg cgtaccgtct tcaaaataag
    10081 aaatctggcg caaacgaaga actgcatctc ctcgttttac ctctaaatag tcagcaattt
    10141 tctcagaagc caacacagca gtaatcgttt gattcgctgg accgatcttt ttcccgcctt
    10201 tttcttctag cgtgcgatac aacgattcag taatctctgc tttgctgtag ccatcgatca
    10261 gcttttgtgg aacactggca acttcaaaac aaatagggac atcatctgcg aaacgaatac
    10321 gttccattcg caagatcgtg tcatctttac tcaagtttag tttttccatt tcacttgagc
    10381 ttggcgaagt caagaaataa gagattgtcc gactagacgg tactcgattt tgtgattcca
    10441 taatttctgt aaaacttgtt gcaccagaca ttttttcttg tactttttct cgcgcaacat
    10501 acgtacccga accgatcttt ctttctaaga ttccttcatc tgccaagttt tgaattgctt
    10561 gtctcaacgt catacggctg acattgaatt tcactgctaa ttctctttct gaaggaagac
    10621 ggtctccgac tttccatata ccctgttcga tttcattttt taattggtca tgaatctgta
    10681 tatatacagg taattgattc gacataacgt acacccgtcc tttcgtttct tctattataa
    10741 ctggtatata cctgattcac aactgaaaaa gataaaggtt tctatccatc aacagaggat
    10801 ttagacacac atcgttctcc ctactcctat tgtcagaaac aattttttag atatgttcaa
    10861 cctagaaaat aacaaaagac cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt
    10921 attattagcg gtttttagcc atccattttt ttgtatgatt cgttgggcgt tttgatttta
    10981 cctggaccgc ttttccgcct tttttcggaa tattcacttc aatattgtat ttttctgatt
    11041 tgaccaaatc atataacttt tcagctgctt cgtctacttt ttctttccat ttcatgtgga
    11101 tccctccaat tcttgtagtt aacttaactc tatcttaatt cagcagaatc ttcaaggaac
    11161 aaggtagcaa agcaaaaaaa agagctctcg aactaacg
//
LOCUS       DAZKOW010000094        10600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000094 DAZKOW010000000
VERSION     DAZKOW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.094"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(238..723)
                     /locus_tag="ACG912_002317"
     CDS             complement(238..723)
                     /locus_tag="ACG912_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFQ0513612.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    813..1158
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1203..1844
                     /locus_tag="ACG912_002318"
     CDS             1203..1844
                     /locus_tag="ACG912_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFQ0513613.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1863..2498
                     /locus_tag="ACG912_002319"
     CDS             1863..2498
                     /locus_tag="ACG912_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513614.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2516..3331
                     /locus_tag="ACG912_002320"
     CDS             2516..3331
                     /locus_tag="ACG912_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0513615.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3650..5086
                     /locus_tag="ACG912_002321"
     CDS             3650..5086
                     /locus_tag="ACG912_002321"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0513616.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5275..7722
                     /locus_tag="ACG912_002322"
     CDS             5275..7722
                     /locus_tag="ACG912_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFQ0513617.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7952..9019
                     /gene="rlmN"
                     /locus_tag="ACG912_002323"
     CDS             7952..9019
                     /gene="rlmN"
                     /locus_tag="ACG912_002323"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFQ0513618.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9009..9215
                     /locus_tag="ACG912_002324"
     CDS             9009..9215
                     /locus_tag="ACG912_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513619.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9212..10315)
                     /locus_tag="ACG912_002325"
     CDS             complement(9212..10315)
                     /locus_tag="ACG912_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFQ0513620.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3509 a         1809 c         2290 g         2992 t
ORIGIN      
        1 aatcaaaaga ataaaattcc gatttataat cgaaaaaaga agcattttta tatatgcttc
       61 ttttttcgta gaatcaataa atatggattt ggctaactat ggaagaatac tcgtattagt
      121 tttcagcgtt catactagtt gttacattgt ttgattggcc aaaagaattt ctgatatatt
      181 ttgtgctaaa taaagaaaaa gacgcttgaa ttgggaaata agaaaactac tctatcatta
      241 caaattttca taaactggca gatgttctaa gtaaacaagt atcttttcaa agtcggaaaa
      301 atccataatc aacgtagtgg tattgatatt tggatgaaag cctacggtat cttgacgatc
      361 aatagcagca tcgatcacga cttggatttt tttctctttg tcgtgcatca atgctaatgg
      421 agtcactgta cctgctggaa cgcctaggag gtcaggaagc tgttcttcag aaacaaaaga
      481 taagcgcttt tcttttaatt gttcagctag ttgttttaaa tcggcgtgct tttcatcagg
      541 caagatgaca agatagattt gttttccttt tcttgatttc aataatagat tttttacttg
      601 cggaccaggt agggaaaaag ggacattttt gactgacgta atcgcaggat gatcgacttt
      661 ttgaaaagag atacccagtt tttttagtag ttcataagct tcttgttcgg ttgcacgact
      721 catcgcgcat caccctcctt tttttattag tgtatcataa aaaagagttg acaaatgaaa
      781 aataatcatg taaattacac gtaaagttaa aacaaatgac atcgaaaaga agagtagccg
      841 ttaggtgttt ttagagagtc tctggttggt ggaaagaggc agcagttaac cgtgaaacac
      901 atcttggagt cgattttctg aagcgtatag taggaaaatc cggggagccc gttacagctg
      961 cggtcgttgc tagatgacca gataaggctg gcttttccgt gaggagtcgg taaaaaaagg
     1021 tggaaccacg tgattaacgt cctttgatct tcggatcaaa ggactttttg ttttcatctg
     1081 aacggcaacg actgtgtgag gttttttctg caaggaaaaa ataaacagag gtggaaccac
     1141 gtataatacg tcctcttggc atttgccaag aggatttttt tattttaaag gaggaaaaga
     1201 agatggagta tttgattgaa gttatgccgc aattattagt aggagcactc acaacattga
     1261 aattattttt atttaccttg attggctcat taccattagg gattgtttta gcattaggat
     1321 tgtctttgca ttttaagccg ctacgctact tgcttcaaac atatgtatgg gttatgcggg
     1381 gaacaccgct gttgttgcaa ctgattttta ttttttatgg gttgccgacg attgggatcg
     1441 tttttgatcg tttcgaagca gcactcattg cctttatctt aaactatgca gcttattttg
     1501 cagagatctt tcgtggaggg atacaatcga ttcctcaagg tcaatatgaa gcagcgcaag
     1561 ttttgcaatt aagtcccttt caaacggtga cgaaaatcgt catcccacaa gtgacaaaga
     1621 ttgttttacc ttctgtaggg aatgaagtta tcaacttggt caaagattct tccttgatct
     1681 atatattagg tttaggtgac gtaatgcgtg ctgggaaaat cgcgatggaa cgcgacgcga
     1741 ctttactgcc tttgctaggc gtggcaatca tctatcttac attgactggt attctaacaa
     1801 tcataatgaa acaagttgaa ggaaaattaa attattaccg ctaactgata ggaggaagaa
     1861 atatgattga attgaagaaa gtgaacaaag catttggtga aaaaaaaata atcaaagatt
     1921 tgaatctaat cattccagat gggaaaacat tagctatcgt aggtccttcc ggtggcggta
     1981 aaaccacgct tttacgtatc ttggctggct tagaaagttc tgacagcgga gaattttatt
     2041 tagacggtgt accgttcaac ccattcgagc ttaaaatgaa agaacaagtt gtaggagtcg
     2101 tattccaaga cttccagcta tttccacatc tgaatatttt tgaaaacatt accttagcac
     2161 caaaaatggt attgaaacag acaaaagaag aatacaccaa aaaagcagaa gaattagcgg
     2221 cttcattagg gattagtgat ttattgaata tctatcctta tcagctttct ggcggacaaa
     2281 aacagcgggt agctattgcc agagcattgg ctatgaatcc acgggtattg gcttatgatg
     2341 aaccaacaag tgctttagat cctgaattac gccagcaagt cgagcgtttg atcctatcat
     2401 tgaaagaaga tggggtaacc caaattattg tcacccatga tttagtattt gcagaaaatg
     2461 tcggtgatca attactaaaa gtttccccaa ttcattaaga aaggagaaga tagagatgaa
     2521 aaaaaccagt tttctattcg ctttgatcgc aggtcttctt ctttttgcag gctgttcgaa
     2581 caaaaaaact tcagcagata attgggcgac tatcaacgaa gagaaaaaaa tcgttatagg
     2641 tttagacgat acatttgttc ctatgggatt tagagataaa gacgggaagc tcacaggatt
     2701 tgatattgat ttagccaaag cagtctttga acaatatggg attacggtag actttcagcc
     2761 cattgactgg tcaatgaagg aatttgaact gaacaatgga acgatcgatt tgatatggaa
     2821 cggctattcc aaaacgccgg caagagaaaa gaaagtgcaa tttacaaaac cttatatgga
     2881 aaacgatcaa gtgctgatca cacctaaaga atcaggaatc acaaactttg gacaaatgaa
     2941 taataagaga ttaggtgcgc aaaatgggtc atctggttat gatgtattta cgaaacaacc
     3001 agaagtatta aaagagatag tcaaaaacca agatgctgtt ttgtatgata gttttaatga
     3061 agcattgatc gatctgaaaa gcagacgggt cgatggtctg ctgatggata aagtatacgc
     3121 agactattat ttgaaacaaa gaaacgaact agaagacttc aacataacaa aaggttctta
     3181 tgagagtgag gattttgcag ttggtgcacg caagtcagat agtacattag tgaaaaaaat
     3241 caatgaagcc ctagatcaac tagagaaaac aggtgaattc caaaaaatat ccgataaatg
     3301 gtttggcgaa gatgttacgc cgagaaatta acaaataggg gactgtgaca aaagacgtgt
     3361 cacagtccct ttttccgaat aaacagtgtt ctcctgccaa ctccttgtga ctcggagctg
     3421 aatggcaggc tcacaacctc ttggtaaacg gtgttcaaaa gccagttttt cggtattaag
     3481 ccaaatttat gcaaaaatgt agaaagccat tttcacaaaa tttttcttac tactcgaaac
     3541 taagcgactt tttcacaacc tcctttacat aattttattt tgttgtggaa ggggtttcgt
     3601 gttattctaa aagaggaacg attgaaggtt ataggaaagg aagagatgaa tggaacacaa
     3661 agaacttacc tcatttccaa aagattttct gtggggctct gcttctgcag cgtatcaagt
     3721 agaaggagct tggcaagaag atgggaaagg agaatcagtc tgggatcgat ttgtacggat
     3781 acctggaaag acatttaaag gaacgaacgg tgatttggca gtagaccact atcatcgtta
     3841 taaagaagat attgctttaa tgaaagaaca aggtctgaaa gcttaccgct tctctgtagc
     3901 ttggacacgg atttttccta atggacgagg agaagtcaat caagctgggc tcgcttttta
     3961 tgagagattg atcgacgaat tgattgaaaa cgatattgaa cctatgttga ctttatatca
     4021 ttgggatctt ccacaagcat tgcaagatga atataatggt tgggaatcac gtcagatcat
     4081 tgaagatttc actcattatg cagaaacatt atttgaggca ttccgcggga aagtccatta
     4141 ctggattagt ttgaatgaac aaaatatctt cacttcatta ggttatttgt tagcagcaca
     4201 tccgccaggt gtgacagacc cgaaacgaat gtatgaagtt aaccatattg ctaatttagc
     4261 aaatgcatct gtcatcaata aatttcacga gttagaaatg cctggaaaaa tcggaccaag
     4321 ctttgcgtat acgccaaatt atccaattga cagtaatcct gaaaatgttc tggctgctga
     4381 aaatgcagaa gacctgatgg ccaattattg gatggatgtc tatatgtggg gaaaatatcc
     4441 gattgctgcc atgaggttct tagaagaaaa aggatgggca ccaacgattg aagctggcga
     4501 tgcagaattg ctagaatctg ctaaaccaga ttttcttggg atcaattact atcaaacggc
     4561 gacgaatgca ttcaatccat taaacggagt tggtgctgga aaaatgaata cgacaggaaa
     4621 aaaaggttca tcagaagaaa ctggagttcc tggaatgtat aaaaaagttg agaatccatt
     4681 tgtcgaacga acaaattggg actgggagat tgacccagaa ggattaagga tcggactgcg
     4741 tcgtatcaca agccgttacc gcatgccggt actgattacg gaaaatggct taggagagta
     4801 tgacaagttg acagaagata aacaaatcca tgacgactat cgaatcaatt acttacaaag
     4861 ccacgtcaaa gccatcaaag aagcaatttc ggatggagca gaagtcctag gctattgcac
     4921 ctggtcttat acagatttgc ttagctggct gaacggttat caaaagcgat atggatttgt
     4981 atatgttgat caagacgaaa cacaagaagg atctttggaa cgatataaga aagacagtta
     5041 ttactggtac caaaaactga ttgaagaaaa tggagagaac ctttagttca catacttaaa
     5101 acaagaaggt acggctggat atggccgtgc cttttcttga taaaatcatt tagaaattaa
     5161 gaaaaactgt aaaataagct gaaaaatgaa agaaagcgtc agttctttca aacaaaactt
     5221 tagttccctt actttttttt gtataatgaa acttgatgaa atgagggata ataaatggca
     5281 caaaagaaac ggcctactaa aaaaaggaaa aagacgaaaa aaacgaaaaa gcaaaaacag
     5341 cagcaggaac aaatgctgac gattggtgtt gggtttgcct tgattttatt tgctgtcttc
     5401 ggctttttaa aattaggttt tttaggaata ttgattgcaa acggctttcg gatcattgca
     5461 ggaaatactt accaaatcct ctgtctttta ttagccgtat taggattttg gattgtcatt
     5521 aagaatacag agttttcaat tggtaaaaac cgacgctggt ttggcggcat actcttttat
     5581 ttcggtatct tattattgtt acatgcccat ttatttggca aactccatac tggggaacca
     5641 aatatcatgg ggacgacatg ggatttgtta gccagtgata taaaacaaag tcaagtagac
     5701 aataacgttg gtggtgggat gattggagca atcctttatc attttactta cttcttgatt
     5761 gcacagcctg gaagttattt agcagcagtg ctattactgg caggaggagc attcctgttc
     5821 agtaatctgg aaggctatca gctgttaaat ggtatccaat caattggaga acgagtacag
     5881 gaattattgg aaggtgatcc tgccaagcaa gctcgaaagc aagcggcaaa ggaagagaga
     5941 atgaaacagc gcgcagaagc gaaagaagca aggcgcttag ctgcaaaaga agctgcagaa
     6001 aaagaagctg cagaatatga gaagaaaaaa gcggtgagcc agcaaaggga aaatactgct
     6061 gatgaatggc aggaaccaga aaatacagag ccggagcagc taagttttgt tccaatcgac
     6121 agttttcaag aaaatatcca tcctgcgaat ctagagaaac cagtaccaga tacaccaaaa
     6181 cagaccaata cagcagaagg tttttcagat gagttgccag aggatgacgg tacctcatta
     6241 gaatttgaga ttgaagcaga acaggaaaat caagactatg aattaccgac agttgatttg
     6301 cttgattcta taccaactgt ggatcaaagt gatgagtata aaaaaattga aaaaaatatc
     6361 ggcgtattag aacaaacttt ccaaagtttc ggtgtcgatg ccaaggtcgt caaagccagc
     6421 ttaggaccat ccgtcacaaa attcgaagtc cagccggctg taggggtaaa agtaagtaaa
     6481 attgtgaact taacagatga tatcgcgctt gctttagcgg cgaaagatgt ccggatggaa
     6541 gcaccgattc caggtaaatc actgattggt attgaagtac caaatggaaa gatcagtatg
     6601 gtttccttta gagaaatcat cgaagcacaa ccaaaccacc cagataaatt actagaagta
     6661 cctttaggac gtgatgtttc tggacgggta caaacggctg atctttcgaa gatgcctcac
     6721 ttactcgtgg ctggttcgac tggtagtggg aagtcagtag caattaatgg gattatcaca
     6781 agtatattaa tgcgtgctaa gccccatgaa gtaaaactga tgatgatcga tccgaaaatg
     6841 gttgaattga atatgtacaa cggcatacct catctgttga cgccggtcgt gacgaaccct
     6901 agaaaagcag cgcaagcctt acaaaaagta gtacaagaaa tggaagaacg atatgaaaaa
     6961 ttcgcggcaa ccggagtaag aaatatttct ggttacaatg aatttgttca acagaaaaac
     7021 ttggaaaacg gaacgaaaca tccaacctta ccattcatcg tagtgatcgt agatgaatta
     7081 gctgatttga tgatggtagc cagcaatgaa gttgaagatg cgattattcg tttagcgcaa
     7141 atggctcggg cagcggggat tcatatgatc ctagctactc aacgtccaag tgtggatgtt
     7201 atcacaggaa tcatcaaagc aaatgtacct tccagaatgg catttgcggt atcaagcggt
     7261 acagattctc ggacgatcat tgattctaat ggggcagaaa agctcttagg gcgaggagat
     7321 atgctattct tgccaatggg tgagaataaa ccgatccgtg tgcaaggagc attcatttct
     7381 gaccacgaag tagaacgggt cgttcaattt gttacagatc agcaagaagc tcattatgaa
     7441 gagaaaatga tgccgacaga tgaagttgaa acgacaggtg ctccagaaca gccacaagat
     7501 gagctgtttg aagaagcaaa agcacttgtc gttgaaatgc aaacggcaag tatttccctt
     7561 ctccaaagaa gatttcggat cggttacaac cgagcagcac gtttagtaga tgaactggaa
     7621 gctcatggag ttgttggccc gtcagaagga agcaagcctc ggaaagtatt cattgagcag
     7681 gaagaaacat cagaaacgtt gccggacgat aatgacatgt aaaacttgtg tgatgaaggg
     7741 ttgaaaaacg aataaccaat aaaaagacaa ggcatgatga taccaaaaga aaaaatggtt
     7801 tttttccatc ttttctaata cggtatgatg tcttgtcttt ttagtttttg tgagattaaa
     7861 gttctattcg atttatttgt gaagtattgc attaaggaaa tgaatcatgt atagtatgta
     7921 gcgaaacttt gagaaaaaga gggaaaatac tttggaaaaa cagtccattt atggcttgac
     7981 gaatgaagaa ttaatcaatt ggtttataga aaacggcgag aaaaaattcc gagctgccca
     8041 agtttgggaa tggttgtatc aaaaacgtgt aagtaatttc acagaaatga cgaatctatc
     8101 gaaacaattg atcgaaaaat tatcggagca ttttatcatc aatcctctaa aacagatggt
     8161 cgtccaagaa gcttcggatg gtacagtaaa atatttgttt gaactccctg ataaaaacat
     8221 gattgagact gtactgatga gacaagaata tggcttgtca gtttgtgtga cgacacaagt
     8281 aggatgtaat atcggctgta cgttttgtgc aagcgggctg ctcaaaaaga accgtgatct
     8341 gactgctgga gaaattgttg cacagatcat gatggtccaa cattattttg atgagcgcaa
     8401 tcttggcgaa cgcgtttcac atgtcgttgt aatggggatc ggtgagccat ttgataatta
     8461 cgacaatgta atggactttt tgcatatcat caatgatgcc aaaggattgg caattggtgc
     8521 tcggcacatt acagtttcca ctagtggatt agcacacaaa atcaaggaat tcgctaataa
     8581 cggcttgcaa gtcaatctgg ctatttcctt gcacgcgccg aataatgagg tgcgaacatc
     8641 gatcatgcgg atcaaccgaa gtttcccaat tgagaaattg atggaagctg tggatgagta
     8701 tttagagaaa acaaaccgtc gaatcacgtt tgaatatatc atgttgaatc aagtaaatga
     8761 tagaccagag catgcgcaac aattagcaga tctattaaaa gataagaaaa aattagcata
     8821 tgtcaacttg attccatata atccagtcag tgaacatgat caatattctc gcagtccaaa
     8881 agctgatgta ttgagatttt atgatgtatt gaagaaaaac ggcgtaaact gtgtgatacg
     8941 taaagaacat ggtacagata ttgatgcagc ttgcggacaa ttgcgaagca agcaaatgaa
     9001 aaaaacagtt gccaaataat cgtatcctac tcgttttttc aattaccata aaaggagcag
     9061 ttttcttaat taaggaactg ttcaacttaa ataagagaaa agattctata gcgaaagcta
     9121 taaagctttt tttgacaaga aaaaccctca tcaagcaaag aaatgtcctc aatttgatga
     9181 cagcctgctt gatgggggtt ttagcaatta tttaactgat tttcttatat ccttgctggc
     9241 tttcttgtgt agcatgataa gtttctacgt caagaatggc ctgtttaaat ttttctcggg
     9301 tgatttcata aggcacatgt ttaatatcat ttgttgccat ggctttatct aagattgctt
     9361 ctaaatcggt cgtgacattg agattcatgt cggttaacga tactggaagt tggttctttt
     9421 tcataaacac atagatttta tcaaacaaat cgaactgctg atccatcatc aataaggtca
     9481 gtacaccata actgacagat gctccgtgaa ggtatccctc cattttttct agaacagtcg
     9541 taccgtaata gaatgagtgt gccatagcag aattatagtt attgttaact aaaacagaaa
     9601 ccaatccggt agtaacaatg atatccgaaa cgacttgttc aattgcatat gagggacgat
     9661 cgtcggcact atctttcaat gcttgcgtac cataagtcaa gagacgatca atgcacattt
     9721 ttccgatttg gattcctaat ctatccgtgt agcctagatc atcgccacga gcagaaaaag
     9781 tagattcgac ttctttggat aaagcatcgc caatacctgc ccataagtat ttatcaggtg
     9841 cttcggcaat aattgctgta tcaatgaagg tgtgtacagg tggttttgaa ggaaagaaga
     9901 gtccgtccat tacgccttct tcgttataga tgacacatac cgcagtgacg ggcgaacagt
     9961 tcgaaccaat agtgggaaag gtgaagactg gtttagctag tttttcacca atgactttta
    10021 cagtatcgca agcacggcca ccaccgactg caaacaggac gtctgctttt tggacacttt
    10081 catgctggat caactgttca atatttccat aagttgaatt acccccgtac cagagactgg
    10141 cgacaacctc tatttcctgt gtcaaagcta cttttatttt gttttctgcc tttgaaagtg
    10201 cagtcttccc tccaatcagc actacttttg tcccatattc tttgacgatt ccatttgctt
    10261 tttcatacgc atctgttcca atggtataat ttggtaagaa aacactttga ctcatattca
    10321 tttccttctt tcttcgttga aacgggttaa aattttaaag agtgatcgat ctgactgcgg
    10381 ctgtagatgg agtagaggtg aacctaagac aggataagtg atgaataagg tggcatgaac
    10441 accttgaaat catcaataag tatcctaaaa acgaacaagt acaaaaacat tgaaaacgac
    10501 aataatgaat ttttgttcct cttagtattt ctaccgattc cctttgcttt tttgtaatta
    10561 tctaaaaatt ctaaaaatta agattattat atgaaaacag
//
LOCUS       DAZKOW010000095        10517 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000095 DAZKOW010000000
VERSION     DAZKOW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.095"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..589)
                     /locus_tag="ACG912_002326"
                     /pseudo
     CDS             complement(133..589)
                     /locus_tag="ACG912_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            754..3116
                     /locus_tag="ACG912_002327"
                     /pseudo
     CDS             754..3116
                     /locus_tag="ACG912_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="ACG912_002328"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="ACG912_002328"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFQ0513621.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="ACG912_002329"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="ACG912_002329"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFQ0513622.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="ACG912_002330"
     CDS             5704..6489
                     /locus_tag="ACG912_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0513623.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="ACG912_002331"
     CDS             6502..7353
                     /locus_tag="ACG912_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFQ0513624.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="ACG912_002332"
     CDS             complement(7477..7929)
                     /locus_tag="ACG912_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513625.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="ACG912_002333"
     CDS             complement(8394..8762)
                     /locus_tag="ACG912_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFQ0513626.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="ACG912_002334"
     CDS             8912..9394
                     /locus_tag="ACG912_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFQ0513627.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="ACG912_002335"
     CDS             9431..10441
                     /locus_tag="ACG912_002335"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFQ0513628.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
BASE COUNT         3576 a         1748 c         2192 g         3001 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaa
//
LOCUS       DAZKOW010000096        10367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000096 DAZKOW010000000
VERSION     DAZKOW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.096"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACG912_002336"
     CDS             6..305
                     /locus_tag="ACG912_002336"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0513629.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACG912_002337"
     CDS             378..1739
                     /locus_tag="ACG912_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFQ0513630.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACG912_002338"
     CDS             1761..2021
                     /locus_tag="ACG912_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513631.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACG912_002339"
     CDS             2035..2787
                     /locus_tag="ACG912_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFQ0513632.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACG912_002340"
     CDS             complement(2881..3486)
                     /locus_tag="ACG912_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0513633.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACG912_002341"
     CDS             complement(3539..4513)
                     /locus_tag="ACG912_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFQ0513634.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACG912_002342"
     CDS             complement(4891..5610)
                     /locus_tag="ACG912_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0513635.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG912_002343"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACG912_002343"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFQ0513636.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACG912_002344"
     CDS             7161..8558
                     /locus_tag="ACG912_002344"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFQ0513637.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACG912_002345"
     CDS             8639..9079
                     /locus_tag="ACG912_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0513638.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACG912_002346"
     CDS             9227..9553
                     /locus_tag="ACG912_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0513639.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACG912_002347"
     CDS             9554..10303
                     /locus_tag="ACG912_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFQ0513640.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3288 a         1829 c         2149 g         3101 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgctt
//
LOCUS       DAZKOW010000097        10344 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000097 DAZKOW010000000
VERSION     DAZKOW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.097"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            121..1542
                     /gene="arcD"
                     /locus_tag="ACG912_002348"
     CDS             121..1542
                     /gene="arcD"
                     /locus_tag="ACG912_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFQ0513641.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            1556..2674
                     /locus_tag="ACG912_002349"
     CDS             1556..2674
                     /locus_tag="ACG912_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFQ0513642.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(2734..4026)
                     /locus_tag="ACG912_002350"
     CDS             complement(2734..4026)
                     /locus_tag="ACG912_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFQ0513643.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            4176..4736
                     /locus_tag="ACG912_002351"
     CDS             4176..4736
                     /locus_tag="ACG912_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0513644.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            4865..5242
                     /locus_tag="ACG912_002352"
     CDS             4865..5242
                     /locus_tag="ACG912_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFQ0513645.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            5175..7247
                     /locus_tag="ACG912_002353"
     CDS             5175..7247
                     /locus_tag="ACG912_002353"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0513646.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(7458..8405)
                     /locus_tag="ACG912_002354"
     CDS             complement(7458..8405)
                     /locus_tag="ACG912_002354"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFQ0513647.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            8651..9115
                     /locus_tag="ACG912_002355"
     CDS             8651..9115
                     /locus_tag="ACG912_002355"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFQ0513648.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(9177..10220)
                     /locus_tag="ACG912_002356"
     CDS             complement(9177..10220)
                     /locus_tag="ACG912_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFQ0513649.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         3447 a         1936 c         2153 g         2808 t
ORIGIN      
        1 aaagttcata atatccaata atatgaataa agttgctttt tgttaagcaa gagcagttgc
       61 aaaatagaag ggaatctttt gcaagtgatt tgttcaattt tataagtgag ggtgatggaa
      121 atggaaaaga aatcgacggg tattagcaaa tggggactgg ttgctctggt tgtcagttcg
      181 tcaatcggaa gcggtgtgtt tgggatcaca agtgatctgg ctagttctgc agctccaggg
      241 ccagcgatcc tttcatggat aatcgttggg attggtattt tagctttggt attatcgctg
      301 aatcatttag gagaaaagcg tccagatcta gatggtggta ttttcggtta cgcggaagct
      361 tcatttggta aattaggtgg cttcatcagt ggttggggtt actggttgtc tgcgtggctg
      421 ggaaatgttg cttttgcaac gatgctgatg agcgccatgg gagaattttt cccgatattc
      481 aaaggtggac aaaatgttcc ttctatttta tttgcaagta tttttatttg gggtctgact
      541 ttattagtaa acaatggagt tgaaggagcg agcgtcatca atacaatcgt gacgatctgt
      601 aaattagtgc cattattcct atttttaata tttgtattta tcgcatttaa agtgaatata
      661 ttcactgctg atttttgggg taacgtatct gataatctag tgaatggaac agggcagcaa
      721 ggaacgatct ggttgcagat taaaggttgt ttgatggtta tgatgtgggt ctttgtaggg
      781 atcgaaggcg caagtgtttt agccaaccgc gcgaagaaac gttcagaagc ccagcaagca
      841 tcgatcattg gtctgctttg tttattgttt atctatatcc ttgcttcgat cttgccatac
      901 ggcgtcttat ctcaagaaga gttagcaact atttcacaac ctgctatggc aaatatttta
      961 aaaggaatcg tgggaaattg gggagcagcg ctgatcaata tcggcttgat tatttcgatc
     1021 attggttctt ggttgtcttg gacgatgctc ccagcagaaa caacgatgtt gatggctcga
     1081 gacggtctgt tgccgaaagg ttggggaaaa acgaataaaa aaaatgcccc aacttattcg
     1141 ttagttgtta ctgcaatctt gactaatcta ttcttattga ctttcttagt tactgattat
     1201 gcctatcaat ttgcctattc tttatgtaca gcggcaattt tgatttgtta tctacttgtc
     1261 gggatctatc aagtccagtt ttctttaaaa gaaggaatca aaaaacaagt agtgatcggt
     1321 gggattgctg tagtttttga attgatgggc atcattttag caggattttc ttatgtcctt
     1381 ttatgtagta ttgcctatct tcctggtttt tgcttctact ggatcgcttg tcgtgaaacc
     1441 aaacatcaaa ttacgggaaa agaaaaagta atgattgggc tgatttgtgt ggccgcactg
     1501 ctttcaatcg ggttgcttgc tgtaggctgg atcaagatat aagggggaaa gaaacatgaa
     1561 aatcgcaaat ttcggtgtag aagaatggct aaataaatgg gaaaagaaag cggtatatga
     1621 tattgcacaa agctcgatcg aagcattgac attagacgaa ctcgttggct tagatgggac
     1681 tagcgtaagt aaatttttcg aaaaacgcaa aaacgaacca ttggactatg gctggatcga
     1741 aggatcagat gttttcaaac aagaagtcgc ttctctttat caaaccgtcg atccggaaaa
     1801 catcttacaa acgaatggag cgactggtgc taatctttta gctctatacg cgttagtgga
     1861 agcaggagac catgttgttt cgatgcttcc tacttatcag caattatatg acatccccaa
     1921 atctttaggt gccgctgttg actttgttca tttgaaggaa gaagaagatt ggcagtttga
     1981 tttagaacag ctagaagcct tagtcaaacc tgaaacaaaa atcatctgtt tgaatagtgc
     2041 aaacaatcct acaggcacat tgctagatcg gaaaacttta gaaaaaatcg cagaaattgc
     2101 tcgtacagta gatgcatatg tattgataga tgaagtatat gcgcctttaa cggataaagg
     2161 tgaattcttg tcgatcgtag atgtctatga aaaaggtata gcaaccaatt ccctatccaa
     2221 aacctactcc attccaggta ttcgtattgg ctggacagct actggacctg agttggcaga
     2281 agtttttcga aagtatcgag actacacgat gatttgcggc ggagtcctgt cagatgatct
     2341 tgctgtacat gcattgaaga ataaagaaaa aatattggcg cgaaacaaaa agatcattac
     2401 agagaacctt gtgattttaa aacaatgggt agccaatgaa ccgaaagttg aacttgtcgc
     2461 tcccaattac gtctctactt ctttcattaa attagccatc gaagaagatg accagacatt
     2521 ttgtatcaat ttattagaag aaacaggagt actattagta ccaggaagtg cctttgattt
     2581 accgaagcat gcccgactag gttactgttg taaaaaagaa accttggaaa agggattatt
     2641 cctcttatct gagttcttga aaaaacaggc ctaaacaaaa acactgcaag gacagtctgt
     2701 gacaactgtt caagcagtgt ttttcattta cgcttattgt ctatgagaag gagagtgccc
     2761 tgaagctatg gagactcctg aagctttttc ttctgcagtt tcttgctgat ggatctcttg
     2821 tgtattgtgt cgtacaagca agaagttgac caaaacaaga agagaagtcg aaagaaagac
     2881 gccgcgataa ccaaatgcac tggaaacact ggagccgatc ataggcccta ctacgttacc
     2941 agttgcttgg aaagattggt tataactgaa gatccgtcca gcagcttgat gaggcgaata
     3001 ttttgtgatc aatgcttgga cagcagggag cagacaagca tcagagatac caatcaaaaa
     3061 acgtaaggca gctaattgcc aaacattcgt gacgaatgcc attgggatat aaaccagaat
     3121 cgcaaaaccc aaaccaatcg caagtattct ttcactccca atcttgtcac ctaagcgacc
     3181 aaatcttgga gcggctatca aagtggcaat cccaggtatc gaagcaatga ttccgctgat
     3241 caacgtaaca ttcccatgtc catgcagcaa ttggcgaatg tacagactga tgattggact
     3301 aatggaattg ttggatgctt ggatgatcat cgttgtgacg aacataccga tgacgacgtg
     3361 tggatatttc aagtctttga aaatctgttt ggcagaaacc atgttttctt ttttcactgg
     3421 gacaaatttt tcatggacga aaaacagact caacaagaag acgaaaaata agatgatccc
     3481 tgtaatgaaa aaggtagggc gatagccaaa cgctgaagca gagatcccgc caagaagcgg
     3541 tccaagtaat gttcctgtaa cagaaccagt tgctaaggtt ccaagaactt gaccactttt
     3601 ttcttttggc gtcccagttg cgacaagggc agtggcatta ctgatataac cagaaaaaat
     3661 cccttgaagc agacgcaatg cgactaattg atacacacta gtcacgcaac ccatcaaaga
     3721 aatgacgatt gccattccta atgaagcacg caacagcatc aattttcttc ctttttggtc
     3781 ggctaatttt ccccaccaag gagagataag cgtagtcacc aagaaagtag atgaaaaagt
     3841 caatccgctc caaaagttca attgtgacgt actataatta cctaacgtat ctatgtaaag
     3901 tgacataaaa ggcatgacta aactaaatcc gataccagcc ataaatgttc caaaccataa
     3961 gacaaacaag ttttgttccc aagtttctct tttcctaaat aatttttcct ttattcttgc
     4021 agccaattag actccttctt tctgaaaatt ttcatctaca gctctttatt ataaactaca
     4081 atatgtagtt tatcaatgaa agtctctttt ttctctttct ttttcgtggt aaactgaaag
     4141 agtgaaggat ttattttatg agaggaaaca tggaaatgaa aaaaagaaat ctgtctcaag
     4201 agaaaatcat cgactgcttt cgagaactag cagaagagat ggatgttcag caaataacct
     4261 ttcagcattt agccaaacgc ttggatatca aggctccctc gctgtataac catttcaaaa
     4321 atattcgtga tgtcaaaaca gcgctaacag cgaaacttct gcaggaactt aatgaccagc
     4381 tgcgacgggc tcttgtggga aaaagtggtc aggaagcact gcagatctat gcagaaactt
     4441 atcgatcctt tgctttttca aatcaagcag tgtatgagtt actgatcagt gtaccgcata
     4501 ccaatgaagc tgttttatta gatggaatcc atgaaacgaa tcagatcatt ttgcagatat
     4561 ttgaggcttt tcaattaacg aaagaagaaa aagttcatcg aagcagggaa cttcgtagta
     4621 tgatccacgg ttatctttcc ttacgttttt taggatactt tacaaaagaa ccaacaatcg
     4681 atccagaaga cagctatttt tggatgatca ataactttat cgctactttg ccttaaacaa
     4741 tcagattcat ttgtaacaat tttaagatgt tcattaaatg acccttttat aagagaaaat
     4801 tcttgcaact ttcttgaaaa gtgactaaga tgagtctaga aaagctgaaa gaaggagcac
     4861 gagtatgtat caagtaatta ccatgttcgg ggacaatgag ccttggtggt tttttgaaga
     4921 ctgggaagaa gatattgaag aagaagaaac attcgagtct ttcgaagaag cacgacaagc
     4981 gtacgaaaaa caatggaacg aaatccatca aaattatgaa tatatcaatg caaaaagtaa
     5041 ctttctcagc gccttttgga atgacggaga tgagcgctgg tgtgaagagt gtgacgatga
     5101 tttacagcaa tacaagggac ttgcgttgtt gaaaaatcat cagcccatca cagtagaaag
     5161 cagaaaggaa ttttatgaaa caactaattc tagcggaaaa accaagcgtt gcgaaagacc
     5221 taagcaaggt gcttggtgct aatcaaaagc acaaaaatta ttatgaagga cccaaggtga
     5281 tcgttacttg ggcccttggt catttattag gattgaaaat gccggaagat ctgaataagg
     5341 aatggcagag ctggcaaatg gacacgttgc caatgatccc taaaaaacta ggaatcaaac
     5401 cacttcctaa aacaggacat caattaaaag caatcaagca gctggctcag cgaaaagatg
     5461 tctcagaagc agtcattgcg acagatgccg gaagagaagg ggaacttgtt gctcgctgga
     5521 tcttagagta tgtgcatttc aataaaccag tgaagcggtt atggatctct tctcaaacag
     5581 ataaagcaat caaaactgga ttcaagcaac taagacctgc gaaagaatat gatgcattgt
     5641 atgaatctgc tcttgcccgg gcaaaagcgg actggctggt aggattgaat gtgaccagag
     5701 ccttaactgt gaagtacgcg gataatttat cagcaggccg tgtgcagaca ccgacactag
     5761 cattagtgag agaccaagaa aagaaaatcg agcagttccg tccacaaacg tatttcacga
     5821 tcacgttgca agctggaaaa gaacaagcca aaatgcttca aaaaaatcaa tttgctttaa
     5881 aaacacaaga agaagcacaa aaacttgttc aattgatcag tacaagcaaa ggaactgtca
     5941 cagaagtcca agaaaaatta aaaacggaat ctgcgccatt gccttatgat ttaacagaga
     6001 tccaaagaga agccaatcaa cgctatggct attctgcaaa gaaaacatta agtcttgtac
     6061 aaagtttgta tgagacccat aagatcgtta cttatccgcg tacagacagc aaatatctga
     6121 caaatgacat gaaaagtacg atgaaagaac gattgcaagc agtcagcgac tttgctcctg
     6181 aggtcaagca gtatttgaaa aacggagcca tcgtccaaca gacaaaagta ttccaagaca
     6241 gtaaagtgac cgatcaccat gcacttttgc caactgaaaa cagagcacgt tatgaaaaat
     6301 taagtaatga agagcgaaaa atctatcaaa tgattgtctc tcgtttttta ggactgtttg
     6361 ctcaacccca taaagtcagc caaacaaaag ttacggttga atttggtaaa gaacgattca
     6421 tttttcggca aaatcgtgtc gtccaagcag gatggaaagg cgaaactgaa actgagacgg
     6481 aaacagtcaa atgggaaaaa ggaatgagaa tcaatcccga tttcgccatc aaaaaagaac
     6541 tatctgcacc gcctaaacca ttgtcagaag caagcttgct tggtcaaatg gaaaaataca
     6601 gtctgggaac cccagctacg cgtgcagaga ttatcgagaa actaatcaaa tctgaactga
     6661 tggaacgtac ccctcaagga ctgcaagtat ctcctaaagg taagcaatta ttagtattgg
     6721 taaatccctc tttagtaaca ccagatttga cagaaagctg ggaaaaagac cttgaagcta
     6781 ttgctgcagg aaagaaacaa gcaggaacct tcttgaatgg aatcgaaaaa gaaacaaaac
     6841 gcttggtgaa tgagatcaaa tcaagcaaac aagaatatca agatttctcg attacccaaa
     6901 agaaatgccc agaatgtggg gccaatctgc gcgagaaaaa cacacgtgac ggcaaaatct
     6961 atgtctgcac caattcagat tgcaactacc gcagaagaaa agatccaaaa gtctctaatc
     7021 atcgatgccc gcaatgtcac cggaaaatgg aaatcatcga aggtaaaaac ggtgcttatt
     7081 tccgttgtaa atacgatgga acgacagaaa aattgcttga taaaaaagag cagaagaaga
     7141 aaatgaccaa acatgaagaa cgacgcttgc tgaaaaaata tgcacaagca gaagaaccag
     7201 aagaaagccc attagcagca gcactaaaag cagcaatgaa gaagtaataa gatatgaaag
     7261 aggttgtgat agaagtggtc agcttcaaga aataagaagg aatttacgaa aattgcttct
     7321 caaatttttg tgaaatttcg gcttatttcc gaagaagcta cttctggaac accatttatt
     7381 cggaaaaaag gactgtgaca agacttttgt cacagtcctt tttcatttaa aaaaatgttt
     7441 acttcatttg acgtatatta ttggtgaggc ttcaattcat ttggcttcac tttttctgtt
     7501 tctcttatga tataaacagg acgctgtttc gtttctaaga agattttccc aatatatttc
     7561 ccgatgattc caagacataa caattggatt ccaccaatga acaggaagat cgagaccatg
     7621 gaaggccatc ctgatgtagg atcaccaaac aacaatgccc gcaaaataat gaacaaaatt
     7681 gcaataccag aaccgacaca agacaaagcg ccgataaatg aagcaatcgt taacggtgca
     7741 tccgagaagt tcacgatacc atcaatcgag tagttgaata atttccagaa ggaccaagat
     7801 gtttcgccag ccacacgttc gcggttttca taagaaatat attctgtttt aaatccgacc
     7861 cagctaaata atccttttga aaatcggttg tattctgtta attctaggac ggcatccacc
     7921 atttgacgag tcatcaagcg gaaatcacga gcgccatcga ccatttctgt atcgctgatt
     7981 ttattgatca actggtagaa ttttttcgca aagaaactgc gtatcgcagg ctcgccttca
     8041 cgagtcgaac gccgggtgcc gacacaatcg atatcagaag tagtgatccg gttgatcatc
     8101 tcaggaagca gttcgggcgg atcttgcaga tcaacatcca tcacggtcac taaatctcct
     8161 gtagaagctt gcaatccagc gtacagagcc gcttctttcc caaaatttct ggaaaatgag
     8221 acaaagcgga cataaggata agctgaggct agcttcttga ttgctgctaa cgtaccgtct
     8281 tttgatccgt cgtcaataaa caaatattcg aactcatggg cacctttgaa tttttcaacc
     8341 tcatcaaaaa aaagaggaac agttttttct tcattaaaac atgggacaac aattgatact
     8401 ttcatcgttt ctatcctttc aatcgtatgt ttatatatgg tttacattca aaatttaata
     8461 aaccttaaat aacaccttta aaatttgatt ttacgtaaaa gaacaggtat tctttttgta
     8521 aactaacgta tttagtttac catgtttcct cggtttatca aacttttaag aacaattttt
     8581 attagttggc aagtagtttt ggatagagaa aaaggaggaa gttccagtct attgactagg
     8641 agcaaacaaa atgaaaatac acacacctat cggcttttta tggttagatg cagatgaatc
     8701 cggattgact gctgtatctt ttcagccaat ttcagaaagc gaatcagcga atcagcgaat
     8761 tttaacgcgc acacaagaac agtttgaaga atattttgcg ggaaaaagaa aagaatttga
     8821 tgttccttta tctataaaga gaggtacgcc ttttcagcaa aatgtctgga aagctttgtc
     8881 gaccattcct tatggagaaa tccgaacgta taaagaagta gccgttgctg ttgatagtcc
     8941 gaaagctgtg cgagcgattg ggcaggcaaa tcgaaccaat ccattgccaa tcgttattcc
     9001 ttgtcatcga gtgatcgggc aaaatggaaa actgacagga tatatgggaa acgcaataga
     9061 tggggttgaa atcaaacgcc aattgttgtt gttagaagga tatctgcaaa attaaaaaca
     9121 gttgtgattc ggacattgtt ccatgcgaga atcacaactg ttttttcatg ttcattctaa
     9181 actttgatca gccaaataaa tagtaaagcc atcactgttt ggagaagccc gacagctaat
     9241 ccataagata agaagcgtgt accttctttc atgaattttg caaagtctaa acgcagacca
     9301 attgccgcta aagcagtgat ctcaaaccag ctgctgataa aatgtgccga ttggtcaata
     9361 actacaggta aagaaaacaa gctgttgacc aaacaaaaga tcacaaaccc taatacatac
     9421 caaggaagcg gtaattttcg ttttttctgc gtatcagttg gcgtttcaaa cagagctggt
     9481 tgtttcttgt gcatgaattt ttcaaacaga tagacaacaa caacgagcat cataatacgt
     9541 agaatcttaa atagcatggc gaattcgaca acagaattgt caagtaagct tgccccagcg
     9601 actacttgac caacagattg gagtgtacca cccaaaagtg cacttttttc aatgagattt
     9661 ccaccaaaca actgtaatcc aagaaaaggg agagtcaaca tcatcacagt acccagtaga
     9721 ttgactagtg tgatgatctg tcctttttct tcctctttcg catggatgga aggggcgatt
     9781 gaagcaatag cagaagaacc acaaacagcg tttcctccag ccatcatcaa agaagaagta
     9841 tcgctgaatt gcagtttttt accaatcaag taagcaccga tgatagtgac tgacatttgc
     9901 agtaacacaa aaacaattcc ttttacacca atttgagcaa tcgtttgagc tgtgacagtt
     9961 gcacctagta aaaaaacaga gaattctaat agtttgcttt cagcaaattt tgtgccgatt
    10021 gctagaacag gctgtttaaa aaaagtattt cctaataaaa tacccaataa aatagcaata
    10081 gtcgctccac ctaactgtgg cagataaata gcaagaagcc tgctgaaggc agcgatggca
    10141 aaagaaagca gtaaaccggg taagatttcg cggaccgctg aatcctttac agattttatt
    10201 ggatgtgaca atgatttcat ttgatcggtt cctttcgtca ataaatacta aaagcatact
    10261 cgaacatagt agcactaaac cagtattttg acaaaagaac aagaggtcgt gacaaaagtc
    10321 ttgtacgacc tcttgtttcg aata
//
LOCUS       DAZKOW010000098        10338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000098 DAZKOW010000000
VERSION     DAZKOW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.098"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            241..390
                     /locus_tag="ACG912_002357"
     CDS             241..390
                     /locus_tag="ACG912_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFQ0513650.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            401..1915
                     /gene="dltA"
                     /locus_tag="ACG912_002358"
     CDS             401..1915
                     /gene="dltA"
                     /locus_tag="ACG912_002358"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFQ0513651.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1912..3117
                     /gene="dltB"
                     /locus_tag="ACG912_002359"
     CDS             1912..3117
                     /gene="dltB"
                     /locus_tag="ACG912_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFQ0513652.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3162..3395
                     /gene="dltC"
                     /locus_tag="ACG912_002360"
     CDS             3162..3395
                     /gene="dltC"
                     /locus_tag="ACG912_002360"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFQ0513653.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            3398..4666
                     /gene="dltD"
                     /locus_tag="ACG912_002361"
     CDS             3398..4666
                     /gene="dltD"
                     /locus_tag="ACG912_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFQ0513654.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4807..5889
                     /locus_tag="ACG912_002362"
     CDS             4807..5889
                     /locus_tag="ACG912_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFQ0513655.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5889..6692
                     /locus_tag="ACG912_002363"
     CDS             5889..6692
                     /locus_tag="ACG912_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFQ0513656.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6762..7502
                     /locus_tag="ACG912_002364"
     CDS             6762..7502
                     /locus_tag="ACG912_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFQ0513657.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7513..8517
                     /locus_tag="ACG912_002365"
     CDS             7513..8517
                     /locus_tag="ACG912_002365"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFQ0513658.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8562..10337)
                     /locus_tag="ACG912_002366"
     CDS             complement(8562..10337)
                     /locus_tag="ACG912_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513659.1"
                     /translation="MILSLLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDF
                     KFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDY
                     QEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKFYLGTTGPLISFIYSQS
                     VPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGI
                     LFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFT
                     PAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQT
                     ALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQ
                     LRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQV
                     TNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPL
                     YRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTG
                     TLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3321 a         1705 c         2093 g         3219 t
ORIGIN      
        1 gaataccatt tatttggaat aagaggttgt gacacgattt ttgtcacaac ctctttattt
       61 tttatatttt ttaaaagtac gagaaattat aaagaaaaaa gcagtgattt gatgtgctat
      121 ttttataaaa gctatgtgac aaaactgtta gtaaaaaaac ggtttctgta agctagacac
      181 atgtttcttt ttgtcaaaat tagttacact ttgtgtgagg ttatatgagg agggaatcca
      241 atgaacaaat ggcgttctaa tccaaaattg aattattggg gaaaattcat cattcgaaca
      301 attttttata gtgcagtact gctgttcctg atttatctgt atcactataa aaatattcaa
      361 ggaggaactt ttatctataa cgaattttag gaggaagagt atggaaatca aaacgattat
      421 tgaagcaatc gatgattggg gaataaatga acctgatcga gttgcttatc aggcggatga
      481 gacgcatact tttggtgaat tgaaacaagc atcagacgca cttgcttatt atttagaaaa
      541 aaaggtagaa ggcgatggac caattgtcgt ttttggcaat ttggagtttg aaatgattgt
      601 ctcatttcta ggggttgtca aatctggcca tgcctatttg ccgattgaag agcatacacc
      661 caaggagcgt atcctatcga tctttcgggt ggctaaacca tctatggtca tcagtatcgg
      721 ggactggata gaagatattc cttcggttcc tgtcataaca aaaaaagaat ttcaagaaat
      781 caagaagata ccagtaggat tttatcccag aaatagcgta aaaggtacag aaaattttta
      841 tattattttt acttctggta ctactggaga accaaaaggc gtacaaatca gtcatgataa
      901 tctagtcagc tttgttcaat ggacgatgga agactttggg atcaagcctg gtatgcattt
      961 cttagcccaa gcgccatttt cttttgattt atcggtattt tctatttatc ctgcattagt
     1021 ttctggcgga atgctgaagc cgttaaataa agcagttgtt caagactttc gtcaattatt
     1081 cgcgacattg ccagccttga agttgaatgt gtgggtttct actccatcat ttatggatat
     1141 ttgcttgatg gaacctacat tcaatggaga aaatgtgccg gaattggaca tgtttttatt
     1201 ttgcggagaa gaattgacga aaaaaactgc agaaagtcta cttgatcgat tcccaaatgc
     1261 acggatatat aatacttacg gtccaacaga agcaactgta gctatttcaa gtattcagat
     1321 tgaccagcat gtactagata gttatgaccg actaccgatt ggctacgtaa aaaaagacac
     1381 gaaagtttct attgtccaag aaggtaaacc tgtttcaaaa ggagaaacgg gtgaaatcat
     1441 tattgcagga ccaagcgtat caaaaggtta cctgaacaat cccgaaaaga cagctgctgc
     1501 gttcttttgt caaaatgact tagcaagtta ccatacaggg gatgcaggta gactagatga
     1561 atatgggctt ttattttatg aaggacgtat ggatcagcag gtcaaattac atggataccg
     1621 gattgaactc ggggatatcg aacattatct gctccaagac aaccgaatca aacaagctgt
     1681 cgtcgtacca aaataccaag gaacaaaagt acagcagctg gttgcttttg tcgtattaga
     1741 aaacaaaacg aaacagccgg attttcagtt gactaaatct atcaaagaac aattactaga
     1801 agttgtaatg gattatatga ttcctcagaa gttccattat gtcgatctgc tgcctcagac
     1861 agttaatgga aaaatcgatc gaaaaaaatt aacagctgag gtgaatcctg aatgatgtta
     1921 cctcatatga tcccttatgc agagccgttt tatttttttc ttttagccat tgcgcttgta
     1981 ccgatcgttc tatctttatt gatatgggaa aaaagattac cagtttatca atcagcagtg
     2041 acacttttct ttttatttgt cagctttgga ggtaattatt ggcaacaggg agcggcgctg
     2101 attgggtatg ttttgtggca gacgatactt gtatggggct attttgttta tcggcaaagg
     2161 aaaaatcaga caggatggtt ttttgccgcc gttattttag cgataattcc actgttttta
     2221 acgaaagtga ctcctttttt tgaacatgga aaagcatcgt tacttggttt tttaggaatc
     2281 tcttatttaa cctttaaatc tgtacaaatg atcatggaaa taagagatgg catgatcaaa
     2341 acatataagc catttcgtta tatccagttt ctgctgttct ttccaacaat ctcttctgga
     2401 ccaatcgatc gctataggag atttgaaaaa gatcagctcc atccgcctgt tcgtgataaa
     2461 tatgttgatc ttttgggaaa aggcgtccaa aatatttttg tcggtttttt atataaattc
     2521 attatcggtt attattttgg tcaagtactg cttcctgtag tgggacgcat tgccttacag
     2581 catggtggta tttcatgggc attagtaggt tatatgtacg tgtacagtat gtatttgttt
     2641 tttgattttg ctggctatag cttatttgca gtcggtacaa gctacatgat gggatatcag
     2701 acaccaatca atttcaataa acctttttta tcatggaata tcaaagaatt ctggaatcgt
     2761 tggcatatga cgctgtcctt ttggttccgt gattatgtat atatgcgatt gatgttcttc
     2821 atgatgaaga aaaaattttt taaaagcaag atcgtaatgt caaatatcgg ttattttgct
     2881 ttgtttcttc taatgggtat atggcatgga ttgacgtggt actacatcgt atatggactg
     2941 tatcatgctt gtctgatctg tctgacagat gcatggatcc gatttaagaa gaagcataaa
     3001 gagcagttgc cttctaacaa atttacgcat ggcttagccg tatttttgac atttcaagca
     3061 gtatgtttta gcttcctgat tttttcagga tttttagata aacttttttt tagctaacga
     3121 atagtgacat attaatttat ataacaaagg agaaaaggaa aatggaagaa caaattttat
     3181 caatcttaga agaaataaca ggaacagatg aagtaagaaa aaatcgtgat attgatctat
     3241 ttgatgaagg gttgcttgat tctttagcat ctgttcaatt attagtcgag ctagacggag
     3301 agctggatat ccaagtccct gtatctgaat tcgagcgaga ggattggtgc acaccgaata
     3361 aaatcattca acaagcaact gcattgaagg gttgagtatg aaaaagaaac tttttgctat
     3421 tttcggcccg gttgttttag ctgttggctt actgtttgtg ttttttacct cctcctatca
     3481 agtagatatc tctaatccag aaacgattag acgtgcctct tcttccatgt cagtgaacgt
     3541 gttgaaagga gatgcgataa aaaattatgc attatctgaa aaacaatata ttcctttttt
     3601 tggctcaagt gaattaagca gaatcagtcc atttcatccg tctgttctgg cagaaaaata
     3661 ccagcgaaac tatcgtccat ttttgctggg agcacccggg acacaatcgt taagccaata
     3721 tatgatgatg cgttctgcag gagatgctat gaaaaacaag aaagtcgtct ttattatttc
     3781 tcctcagtgg ttcgttaaaa atggtgtaaa aactgattac tttaatacgt attattctga
     3841 acttcaaaca tacgactggt tgttttcaat gaagaaagta acgcctgctg atcgctattt
     3901 agctagaaga ttattaactt tctcaaaagt aaaagagaat gatacgttga cggcgattct
     3961 gcaaacgatc aaaaaaggga agctaccttt acctgaatct ttgaatcagc tgcgcagcca
     4021 atggaacatg ctgaagagag aggatgaagt attcagcaat attgggttga aagatcgaca
     4081 gcaaaaaatc gatcatgagt caaaacgatt accaaaacaa taccaagaaa cagaattatc
     4141 cattttagca aatcaaattg gagaacgaga aacgacgaat aatccattcg ggttgaaaaa
     4201 cgatttttat acgcatcgta tccgagccca cgagccagag ctcaaacagt cacaaaaaaa
     4261 ttgggattat cgtttttcgc cagaattttc tgattttcag ctggtcttgg atcaattagc
     4321 aaaaaaccat aacgaagtat tgttcatcat cccaccggtt aacgagaaat ggagcgatta
     4381 taccggacta tctcaagaaa tgttacaagg atttgccaaa aagatcaaat ttcaattaaa
     4441 tagccaagga ttcaatcgaa tagctgattt tgtcaatcaa gcgggtacca actattttat
     4501 ggaagacacg atccatctag gctggaaagg ctggttggct gcagatcaac agatcagacc
     4561 gtttttagaa gaaaatcata ttactgctag taagtatcat cttgacgatg ctttttttag
     4621 caaatcttgg caacatcaaa taccagataa actacaatta aaataaagaa aacaggcgag
     4681 tgttgctcgc ttgttttctt tatttttgaa tattctgaat aaaagataaa aataccaagt
     4741 ttgcctgaac aaaaatcata agcaatggta taatcacaat atgaatatag aaatggggga
     4801 aaacatttgg atacaaaaga agaacagcta cttattgatt taacttctgc aaaaggagta
     4861 cctggaaatg aagaagaggt aagagaagta ttcagagaat atgccaaacc attcgctgat
     4921 gatatttttt atgacggttt aggcagtgtg attgccaaac atggtgctgg cggaggcccc
     4981 aaggtcttta tttctggaca tatggacgaa gttggtttta tggtaacaaa aataaccgaa
     5041 aaaggattct tggaattcca aactcttgga ggttggtggg ggcaagtcat gcttgctcag
     5101 caagttgaaa tcaaaacacg tgaagggaaa atcgtccacg gtgtgattgg ttccaaacca
     5161 ccacatgttt taacacctca agtaagaaat aaaccttatg aaataaagga tatgttcatt
     5221 gatattggcg catcaagcca agaagaagcc aaagagtggg gcatacgtcc tggagatatg
     5281 gtcactccat atattgaata caaacgaatg aatggcagta aatatttact ggcaaaagca
     5341 tgggataatc gtatcgggac agctgtttct ctgcgagtat tagagaatct atctaaagaa
     5401 gcacacccaa atgttttatt cgcaggaagc gatgtgcaag aagaagtagg cttgcgaggt
     5461 gctcgaacaa gtacacacct tgtaaatccc gatattgctt ttgctttaga tactggtaca
     5521 gcaggagaca ctccaggaat gacaccaaaa gaagcagatt cgattttagg aaaaggccca
     5581 cagattttga tttttgatgc atctatgatc ccgcataaaa aattattgaa ttttgttatt
     5641 gatgtagcag aggaaatgga tattccattc caatatacag tgatttctgg cggaggaact
     5701 gatgcaggac aaatgcattt gacaagagat ggtgttccgt ctgtagccat taccgttcct
     5761 gttcgttatc tgcattccca tacatcagtg attcatgaag atgattattt gaatacagtg
     5821 aaactagtca cagaattagt gaaacggctt gatagtgaaa cagttgaaaa aattcggagt
     5881 tatgaataat gaataataga atacctattt ctgaatatcg gaaaaaagca cgagtttctt
     5941 tggaaaatca atggggaatc aatacttggc ttgttttctt atctataata tttacgagcg
     6001 tcatccagaa cttttttgac ggtttcagtc cttttccgga agaaagcatg cagcagcact
     6061 tattgagttt tctagtcgat aatttacttt tattcgcatt caactatgca ttgttttaca
     6121 ttgcgctagt agttgtacgg ggtggcagac caaaaagtaa ccttttattt tcagttttcc
     6181 aaagaaagta ttttggttcg atacttggca tcaatttgat caattcagtc atcaactggc
     6241 tactcaacag tttaatcttt ctgcccatgt ttttaatcag cggagtgagt acttatatgc
     6301 agctgctttt cagttcaaat gcggtaccta cgggtgtcat gccagaagat tttcttgata
     6361 tcagctttgt tttatcgatg atagggatga tttttgtttc agcactcatc atgagtattg
     6421 ttggcggttt attccaattt gctgcatgga caaagttcga ctatcctgat ttgtcggtta
     6481 ttcaatgttt gaaatatggc tggtttttat tgaaagatag aattggtacg tatattttgt
     6541 tgcagctgtc ttttatcggc tggtatttgc taggagcaat cgctttgttc atcggtcttt
     6601 tgtgggtcat tgtttatgtg aatgtgtcag ttgcagaatt ttatgaacaa gcacggattg
     6661 aaaaaggagg tccagacgcc tattttactt agcatgtaag cagtttattc atcaaaaaaa
     6721 taacttttgt ggtaggataa atctttgagg gggaatgtac gatgaaatta actgttttag
     6781 gatgcttagg agcgtatcct tataaaaatc aagggacaac aggttatttg cttcaatctg
     6841 acgggttcaa tttgttgatc gatgcaggta gtgctacgct gatcaaatta caagaacacc
     6901 ttgatccact tgatctagat gctgtgatcc ttagccatta tcatcatgat catattgctg
     6961 accttggtgt attacagtat tactggcaac ttcatcctaa gagaaatccg gagacattgc
     7021 tttcaattta tggacacaca aaagattcca ttcactttga agaattgacg ttggaaaatg
     7081 tctcaaaagg acatccttat tttgaagctg aagaattaaa aattggtcct tttctgatta
     7141 cttttatgga aacaatccat ccagtagtat gttacgccat gcgtatcgta gaagaaacaa
     7201 cggggaaagt attcgtattt acaggtgatt caggttattt ggaaagtttc gctgattttg
     7261 cgaaagatgc tgatttgttt ctagcagata cctatctttt tgaaggaaat gaacatcatc
     7321 atgcccattt cacttcaaaa gaagctggag aacttgcgaa atcagctaac gtaaagaaac
     7381 ttgtacttac acacctacct cagtttggtg atttagaaca attgaaaaat gaagcgcaaa
     7441 aggcagcagg caaagggatt gatatagaac ttgctgcagt tgacaaagtg tttaacattt
     7501 aggaggaaga acatgatttt tgtaccaaat gaaaaccacg acccaagaat caatttagca
     7561 attgaacaat atttgctgca agaaatgcct ttagatgaac ctattttgct cttttatatc
     7621 aatgaacctt caatcatcat cggcagaaac caaaatacgc ttgaagaaat caatcgagat
     7681 tatgtggaag aaaatggcat tcatgttgtc cgtcgcttaa gtggaggcgg agctgtctac
     7741 catgattttg gaaacctgaa ctttagtttt atcatgcctg atgatgggga ttcattccgt
     7801 gactttgcga aagtaacaca gccaatcatc caagcacttc atgaattggg tgttgctggg
     7861 gcggaattga aaggccgaaa tgatctagtc attgatggca tgaaattttc tggcaacgcg
     7921 atgtatgcta ccaacggtcg gatgtttgct catggaacaa ttatgttcga ctctgatatc
     7981 aatgaagtag tcaatgcatt aaaagtaaaa aaagacaaaa tcgaatcaaa aggaatcaaa
     8041 tcgatccgtt ctcgtgtaac gaatatcaaa ccatttcttc cagaagaaaa gcaaaaaatg
     8101 acaacagaag aatttcgtca agaaatcttg ttgaaaattt tcggcgtaga gtcagtagaa
     8161 gaagtaaaaa cgtacgaatt gactgatgaa gattggaaaa aaatcaatcg aatctctgag
     8221 gaatattatc gaaattggga ttggaattac gggaagtctc cagatttcaa tttctctcgc
     8281 caaaaacgtt tttctatcgg ttcgattgaa gtccatttga atgtctcaga aggaatgatc
     8341 aaagaagcaa aaatctttgg cgatttcttc ggactaggag atatctctga cgtggaaaaa
     8401 aaattggttg gacagaaata tgataaagca tctctatcaa aaatagtcag ccaaattgat
     8461 gtgaagaaat attttggtgc gatagaacca gaagaattgc ttaccttact ttattgaaaa
     8521 taaaaaatag cggaatccac atatatggat tccgctattt tttatacagg attcgggtaa
     8581 tttcgagaga gttgaacagt gatccgatca atgaaagtat ctttttcctg cgttagtgtt
     8641 cctgtgtaac gatccgctac gtctttcatc atcattaagt caaaacgttc ctgatcatct
     8701 aattcttctg tgatgcctcg actaaatagg tttgtatcgt aatgtgcact atgttcgcaa
     8761 caaatgacga aatgttcctc ttgttcgtgt atcgacaaat aaatttttcg atacagtgga
     8821 ttttcgatgg catgagtttc tctaaaggaa tgctctaaag cgtggatcaa aagttgagcc
     8881 agatcgtcat cgttgatagg aagttcttcg ggaagatctg cttggaaggt tatctgaata
     8941 ttccgtttgg cagctaactt ctggtaacgc gctaagataa gatttgtcac ctggtggttg
     9001 caaaaagaac acagcatttc gaaatttttt gcttcttcta atatcttttc cacatagttt
     9061 ttagcttttg tttggtccgt atgcattaaa tcttgcagat tctccatctc ttggacaaat
     9121 tccaggcgaa gtgtatttac ttcttggata tgctgtcgaa gctgctcata atgttgctcc
     9181 aacaaatcat ttttgatttt taagaaatct acttgtctcc gatctttgtc ggtatttaag
     9241 atgtgatcga ttagattgta gctgataata acgattagaa tgataatata taaaatcgta
     9301 gaccaattga tcgttgtttg atagatacct aatgccgttt ggatgtaaat caagcaatgg
     9361 ataatggcta ccaagacgat ccaaggtgta caagctacat aaaaacggtt attcttatat
     9421 gcttcgccaa tgcatgcaag tgaagtgact aaagtgctta taacactagc aatattagca
     9481 atcggcatta attcaggaag ttctgcagga gtaaaagttg cataaagcag aacgccgata
     9541 aaaatcaaaa gttggaacgt acaaaaaatt ccgtaccatt ttttatagta aaacattcgg
     9601 ctccaatagt agcagatgat gaaagctgac gttaagataa taaataaatg agataaaaaa
     9661 gaatgaacga atggttcaaa aagaatacca cttaaaatat cttctgatac agcttcaaat
     9721 cctaatgcca aagcaaagca acttaagaga ataagtgagt attcaagacg tttttgttta
     9781 tacatcaagt agatcgtata gaaaaaggtc cctatagcga taaaaacagc gattatcatc
     9841 gtcaatactt gcggcactga ttgtgaataa ataaaactaa tcaatggtcc agttgttcct
     9901 agataaaatt tcggtggcag tccagcatag ttctgataag gagatgagac tgtaagggtc
     9961 aatttttttc cgatataatt tgctggtaaa tctatcacag ccaatgagag tccgggattg
    10021 ctttcttcct gataatcttt ccgttggtat aaaatctttt cgcctagata tacagtaatc
    10081 cattgatggt ttcctttgat tagtaaattt gcttcgtcca acggtttttc aagtgtcgtt
    10141 tccataatga atgtttcgtc tttatccacg gtaggaatcg tacgagtgta cttgctgtca
    10201 aacttaaaat caggatcatc ttttgtatag aaaacccacg aatcttttat ttctttcatt
    10261 tgattgctgt ccgcaggaga aaaaataaaa taaaagaaat aagcaattcc tgttaaaaat
    10321 aacaatgata atataatg
//
LOCUS       DAZKOW010000099        10313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000099 DAZKOW010000000
VERSION     DAZKOW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.099"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            425..1660
                     /locus_tag="ACG912_002367"
     CDS             425..1660
                     /locus_tag="ACG912_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFQ0513660.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2659..2919)
                     /locus_tag="ACG912_002368"
     CDS             complement(2659..2919)
                     /locus_tag="ACG912_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFQ0513661.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2946..4247)
                     /locus_tag="ACG912_002369"
     CDS             complement(2946..4247)
                     /locus_tag="ACG912_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0513662.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4539..4997)
                     /locus_tag="ACG912_002370"
     CDS             complement(4539..4997)
                     /locus_tag="ACG912_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0513663.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(5010..6341)
                     /locus_tag="ACG912_002371"
     CDS             complement(5010..6341)
                     /locus_tag="ACG912_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFQ0513664.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6352..7794)
                     /locus_tag="ACG912_002372"
     CDS             complement(6352..7794)
                     /locus_tag="ACG912_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0513665.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(7781..8545)
                     /locus_tag="ACG912_002373"
     CDS             complement(7781..8545)
                     /locus_tag="ACG912_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFQ0513666.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8649..9524)
                     /locus_tag="ACG912_002374"
     CDS             complement(8649..9524)
                     /locus_tag="ACG912_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0513667.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(9779..9863)
                     /locus_tag="ACG912_002375"
     tRNA            complement(9779..9863)
                     /locus_tag="ACG912_002375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9827..9829),aa:Leu,seq:caa)
     gene            complement(9881..9952)
                     /locus_tag="ACG912_002376"
     tRNA            complement(9881..9952)
                     /locus_tag="ACG912_002376"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9918..9920),aa:Gln,seq:ttg)
     gene            complement(9962..10034)
                     /locus_tag="ACG912_002377"
     tRNA            complement(9962..10034)
                     /locus_tag="ACG912_002377"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9999..10001),aa:His,seq:gtg)
     gene            complement(10096..10180)
                     /locus_tag="ACG912_002378"
     tRNA            complement(10096..10180)
                     /locus_tag="ACG912_002378"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10144..10146),aa:Leu,seq:caa)
     gene            complement(10201..10271)
                     /locus_tag="ACG912_002379"
     tRNA            complement(10201..10271)
                     /locus_tag="ACG912_002379"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10237..10239),aa:Cys,seq:gca)
BASE COUNT         3330 a         2151 c         1592 g         3240 t
ORIGIN      
        1 ttgatttctt tttgtgttat ctgaataatt atcgtttaca gttataacac atagtgaaaa
       61 ttgtcatggc gagaaagtct aagaatattt ttaaacataa ctatttcttt cttacgtgtg
      121 tataatgacg acagcacacc tatcaattaa tataattatt ttattttttt atgttctcat
      181 gtttattttt aatgacacaa cttctcgtat ttatctggca caacatataa atcgatcaaa
      241 aaattgttgc gtgttttttt gaaaattgga gtttcaactt gattacaaaa tttaaattta
      301 gaagttgtga aaacagcgag agacagttta atattcaaaa tatacatagg ctactcgaac
      361 gctgtgtttt ttagtatact agtaagaaac gaagggatag actcattcca ataaggagga
      421 tttcatgaag acaattcttt ttgctcctgc agtattcaat ttagctgaga caacacgaat
      481 gattgaggtt gccaaagcta tgaaagaccg ttattactgt gtattctttg gcttttctaa
      541 aacttttgct cctttgatca cagaaagtgg ctttgagtat catttactag cgccgatcct
      601 tacgaaaaaa caagaagaac aaatcataaa atttgaccag ttgcgtacac ttaaaaaccc
      661 ttttaccaaa gaaatggttg cctctcgtat cactagtgaa cagagtctga ttgctaagtt
      721 tgagcctcga ctgattgtga caggttcgaa tgtgacaatt tttttatcgg cccgcaataa
      781 aaagattcct ttagtatatg ttaaacccta tgctttgagt gaaccttttt ttcaatcaga
      841 gcatgcgctg atgccccacg agttacgttt cttcggccct ttaagcaatc ccctttggca
      901 ctggacaaaa aagcgtatag taaatacccg atggatcccc aatgccttca aacacgctgc
      961 acaaaaagaa atgcttcctt taccaaaaac aagtttacaa atgatggatg ctgatctaaa
     1021 tctcatcaca acgcctaaac tatttttgga agaagaacag ctgccggaaa attatcgaat
     1081 cgttggtcca atttttgcta agctcagtac ttccttgcct gaagaagtgc taacttttat
     1141 acagcgaaag caacaagaaa gaaaacgtat tatctatttc gcaatgggta gttcaggcaa
     1201 tccgcgtatg ttacgcaaaa ttttgttctt cttacaaaaa caaccggatc tagcggttat
     1261 tgcacctatt gactatctgc ttgatgacat tcaatctgac tcagaagatc ttttaataac
     1321 agaatatctg tctagtcatc ttttgcatga attcattgat ttttctttta tccacggcgg
     1381 ggaagggacc gtccaaactg cctgtgaatc cggcaaaccg tttatcggtg tggggatgca
     1441 atttgagcaa agctgtaata ttgactattg tgtaaaatac ggaaacgcaa tcgccctcac
     1501 aaaaccagtg actgaaagaa aactgcagat tgctctgcgc gaagtttctt cacccaagat
     1561 tcgtcagcag gcacgcacgc tgcaagccgt ttttccaata aatggtccac aacaagcaat
     1621 tcaagaaatc gaaaccttcc tgagcaacca cccaatatag tttcaagtag aaaaagcttt
     1681 tttaatagag actcatcagt aatttcgcta ttcgagaccc agacagtgaa aatacaaaaa
     1741 aagtaaatag tggattgaaa agttagagtt acttaaatta cggtgtctaa aaaacacaaa
     1801 atcatcctta aaataaaaga tctgacgcta tccaaatgag cataggtagc gtctgccatt
     1861 tattcttagt caaattcttt gacacctttc tctggttgga tcatctcaac gtttcttcaa
     1921 aagaaagacc ataataatta ggcaaataca ttttcttaaa atgtgtcata tagatctaga
     1981 agcatcgctt tgtagagatt tgtgccgcta gtcccctcaa caattactaa atcaaatgta
     2041 ggatttacca ctttatcttt gataatcaac ctccctcttc tgttgagaca atgatctcgc
     2101 aatccgatgc atactcctat ataaactgcc agcaatcccc acatattgag tagatcatgt
     2161 cagaatgttc tatgtcgtca gcaacgatac actaaaagtc acgttcacca tgactgattg
     2221 ctattcccag cacaattatt tctatacacg tttcatgatt tgaataaaca ctcacatcat
     2281 acatattacc attcatacac catactgcag cccctactat atggttgtaa gctcccaaat
     2341 ctatatttga cttgatcact ttggtagtag atctactaat tctaaatcca ttgctgataa
     2401 aatttccatt ttttcctcct tctttctgcc aaaacagtta aaacaataat aatctttatg
     2461 taaaaaaggg ctgtgacaaa agccttgtca cagtcccata ttccgaataa acggtggtca
     2521 aaagcttgca tctgcggtaa taagttcaac caaacgaaaa ataagccaac atttcacaaa
     2581 aatttaagaa gcaattttcg taaatttctt cttatttctt gaagctaacc acttctgtta
     2641 caaccacttt tagaacattt aaagcttcga tgtgacaaac catctatgct gctcgatcat
     2701 aaaattgata gcaaaaattc cacaaagtac atatgtcccc catgaaaaca aaacattcat
     2761 atgcggtaca atgatctttg gaagaatcac gatcaaaaat gatgccagtg cccaaatcca
     2821 tcgattgatt tgaatatgat atgtagctag catattcaat ataaggtgac tagccgttaa
     2881 taaaaagata aaaacaaaaa ataaaatcag ccaatacatt gtttcccacc ttctaaaaac
     2941 caaacttaga cagtctggac ttcgttttct ccacgacttt gttcatctga cagcatcttt
     3001 ttatcataag cttttacaaa tggataatag ataataaatg ctatcaatgt gttgatcatg
     3061 atcaagataa caacacgcca gtcatttcct gctgaaagat aaccgccgat tggagctggt
     3121 aatgtccatg ttggcaaaac agtaaatcta ttgaccattt ctaatttgac tgcaaaataa
     3181 ctgataatag tcaaaactaa aggaactaag ttgaacggga tgaacatgta aggattcaac
     3241 atcataggag ctccaaaaat tatgggctca ttgatattga agataccagg aataatagaa
     3301 aaacgaccaa cctctttcaa gaattttgat ttacagaaga atataaacaa aacagatacc
     3361 acgatcgttg ctcctgcacc accaattgtt accgtccatt gataaaactg ttcaggagcg
     3421 atatagggta attgggtggc agctgtacca tctgccaatg cctttgcatt ctcatccaac
     3481 atgatcagcc acattggacg aacaacagcc cctacaatac tcatgccatg gataccaaat
     3541 gaccacaaca aatggatcaa aaacattggt agtaaaacac caagcaaatt attccctaaa
     3601 atattggtta aaggctcgaa taacgataat aaggcagcgt ttacatcaaa gcccgcgacg
     3661 cttcgaatca gccaagtaat tgttaaaatg actgcacctg gaatcaatgc ctcaaaacta
     3721 cgggcaacag caggtggcac ttgttctggc attttgatag taatatttcg atttttaaag
     3781 aaacgatata cttccactgc aaaaatcata gataagatcc ccgcaaacat cccagatgct
     3841 cccaaagaac tcataggtaa gacccagcca agaggtgcct cttcaggagt taaaacgttt
     3901 acaggaactt gtaacataaa gaacgttcct aaagataata taccacctgt cacactatct
     3961 aatccatatg atttagctag atccgaaccc ataacaaaag ttgcataaat agccatcaat
     4021 cccatcgtca tcctaaaggg taaaacaatc gttgccgaat aaggggcaat aaattcattc
     4081 catccaggaa acggtaaatt gataaagacc ataaaaaatg tcccaatcaa aatcaatgac
     4141 aatgttgaaa caaccccatt acgaattgct aataaatgac gctgtgtacc aatttttgcc
     4201 attggtggga caagtttctc ttcgacccat ttcatcagct tttccactaa aaccactcct
     4261 tttcaataaa atatgaacca ttcattgcat gacaacgcaa agaatgttca taactaatta
     4321 tataattaag aaattttatt tcaatatatt ctcttaaata atcaaattta tttttaagaa
     4381 tcctgaaaat aagttttcta aatcatagga gcaacaaaaa aattcgataa ttaacaaaaa
     4441 tcaaaatgat taaatctagt gattgaaaca tatcatttca aaaaaatttt gtaaattcac
     4501 ccataaatat caccctattt aacttttttc atcgtacact aaaattcaaa acaactatct
     4561 ttaatcttta cttcttggtt ttgttttaat aaatttcctt caaaaatgcc tggagaaact
     4621 acaatgatca ccgtatcttt ttgtgcttgc tccaacttct tcaaatctat aatagctaac
     4681 agtgtccctt cttttacctc ctggttactt tcaacccgta tctgaaacgg acttccttcg
     4741 agttcaactg tatctaaacc catatggatc agtatttttc tgccagattc actttcgatc
     4801 gttatggcat gtcgggtagg aaatatatta tcgattattc ctttaactgg cgcatggata
     4861 tatccatcat gattcgttat agcgtatcca actcctatca tttttgaaga aaagacctcg
     4921 tcttttactg ctgttaatgg aataatttga ccactagcta cagcttttag aatgtttctt
     4981 gttttaaaga aatgcacttt ttatcacctc tactttaatg ctggccaata ttttttgttt
     5041 actttgatca tttcatctag tatttttttt gcgactctgg catttggtac ggtttgattc
     5101 aatgtaaatg cttgtaaagc tttttgataa gaaccttcaa agtaagcatc tactagtaac
     5161 ttctctactg caacttgtgc ctccatcaat cctttatgaa aatcactgat tggttttctt
     5221 agtgtaattg gctccacacc agttgcacca acatatgccg gtatttcaac aactgcatct
     5281 gaccgtagat tcggaatcgc cccttgatta ggaacgatta gcataaagcg acgacgatca
     5341 tcattcaaca aggaaatagc tatatcgacg atatattgtc catgctcccc aaaattaaag
     5401 ttcaatactt cttccacaat accgcggcgt attttatcag ccatttcttt ggtatttttc
     5461 tctcgtccat ccatgaccat attagcacgt gtatactctt tgtcagtatg ctcaacgacc
     5521 atatcaggat ataagtaata ctctaagtaa ttattcggga tattacttgg aaaatttttc
     5581 gtgatgactg acatcatttg aaaggttttt tgccaagttt tatctcctat attaaaatcg
     5641 gcgacttgca tttcttttgt tattagtttt tcaataatct ctggcataat ttctcgttgc
     5701 aattctttat cataaatcga tgtataccag ccaaagtggt ttaaaccata atacgtaacg
     5761 atccaattct tacgatcata accgtaattc actgcaatcg tttcttcaat tgaaatagtc
     5821 atatcacagg cattaatcat ttttacattc ggatatttac gacgaacagc ttcagaaacg
     5881 atcgactctg gatttgtata attcaaaatc catgctttgg gggcatagtc ttgtatatga
     5941 tcgacaattt ccaataaacc tttgattgag cgcatcccat aagcaaatcc tccgagtcca
     6001 cacgtttctt gtcctactaa tccatgttta agtggaatct tttcgtcctt ttctcgcatc
     6061 tccaatcctc caacacgaat ttgtgagaaa acaaaatcgc atcctgtaaa agcaagcttt
     6121 ggatcatccg ttgcacgaat gcgaatattt gaaaaacctt ttcttttcag cataaattta
     6181 acaatcaagt acatatccat atttctttct tcatcaatat catataaacg tatttcagat
     6241 aatggcaacc gtttttcatt gttcaaaatg gcttgaacga ttcctggtgt atacgtactc
     6301 ccgcctccag ctatagtgat aatctgtttt ttcatcatca tcttttcctt tctattcttc
     6361 taataattta ttgaattctg ccataacata agtcacttct aagccgatga taatttgata
     6421 atttttttca gttggtttaa tgatcccgct tatccctaat tgcttgattc gctgttcctc
     6481 gaccatggtg ggatccacta cctctacccg taggcgtgta ttacaactat agacatccag
     6541 gatattggct tttcctccaa acgcttgtag catttttgtt gccatatatt cataattttt
     6601 acttgctaac cctaactctg tttcttctaa ggttacttca ttgccaaact ctgccttagc
     6661 ttcacgtcct aatgtctgaa tgtttctctt attgatcaaa taataaaaag taacgtaata
     6721 caaagcaaaa aagaaaattc cgattggaag aatcaaaata gcattggtag cgattcgaaa
     6781 attgatgaga taatcaacaa tacatgctcc gaatgtaaaa ccaattcgta tattgaataa
     6841 gtaacagaca atccctgcta aaccggcaaa aagtgcatgg atcacgtaca acataggaga
     6901 aagaaacatg aaactaaatt caacaggctc agtgatattt gcaataaatg aagttcctgc
     6961 accgcttccc atgattcctt tgaccatctc tttattttct ttataagctg ctttatagat
     7021 agctagacat atagctggta taccaaacat cattactaca aaaaaaactg ataaaaacag
     7081 tccagcctga ggatctccgt tgataaagcg tgtcatctct cctctgaaaa cttccttgct
     7141 aggagatgta tagcttccta aatcgtaata aatatacgta ttcaacacat ggtgcagtcc
     7201 aaaggggatc agcaaacgat tcaaaaacat aaatatcccc acacctaatg ctcccatatc
     7261 aacgagcagt tttgcaaaat tatcaatccc aatttggatg agcggccaga taaagcccaa
     7321 aaaaacagct gcgattgttt gtagaatcat caccatagtc aatgggaatt tttcgcccgc
     7381 aaaataagaa aaaactactg gtaatttttg ttctttaaag cgattatacg tccatgccgc
     7441 tactaaaccg gaaataattc caccaaaaat ccccatattc acttctgggt ccataatttc
     7501 taagcctttt ttcaaggtag caatcgctaa aaaaccagta agtgcaggta ttcctttgtc
     7561 cttatttttg acaaaaccga ttgcaactcc tatagcaaat aagatatcca agtttccaaa
     7621 aacggcattt ccagctgcac caattactgg gatgttcagc atatcgtcag ctgataaacg
     7681 catgatcaat cccgctaatg gcatcgctgc aactggaagt agcatcgctc ttccaagctt
     7741 ctgcacattt gatttaacat tcatttttat tcctcctcta ttaaatatcc tcatatgtta
     7801 ctaactcaat caaaggatca tttttcactt tttgagcaaa tgctgaatga attaaaaatt
     7861 cgacttggtg ggtacgagga tacacaaaac ttgaaccatt taacaattcc tgatccaaat
     7921 acgctgcgtg acacatgact tcgatcgttt tatcttctct caatatcatg agtaaacttg
     7981 ccaaatattc ttccacactc gtattgattt gtcgtttttt ttcatcagtt tctcctacga
     8041 catcgaaaca aggctcaaaa taatcgacat gacgaacttc atcttttcgt tcaaagttgc
     8101 tccgaaccgg caaatcatat ttttttgcta atgtaacaac tacctcctga ttttcatcca
     8161 aagtatgagt atgatgatga ctgtctaaat gagttggtat gatccccgca tgataaattt
     8221 tttgaatctg agcattccat tcctgataca gttgatcttt gtcaatgtta aatggatgct
     8281 gataataaga taaggatctg aactctccgc tttcgataag agtatcgaca gttttgagga
     8341 gtggtttgcc acacgtcaat gttaaatgga ctcctacacc tagcctaggc atttcctttc
     8401 ttagtttcac agcatgctcg aaacccggca tatttgccat caatgtagtt gatgtcaaaa
     8461 ttcctcgctg ataagcatcc ataatcccat aattcacacc atggctatag ccaaaatcat
     8521 ccgaattaaa aatcactttt cccatttcat tctccttctt ttttctacta aaataggcgc
     8581 aaccaaataa tcatagtcca tttactatgt gtcatttggt tgcgcctata gatagttacg
     8641 cccttatttt aatagattct tgctagatga attgccaaat agatgatttc attttcttga
     8701 atggcaagtt gaaattgctg ttcgatgata tctttaatct ttagtgctgt acaatacgct
     8761 ttgctatgtt taaatgtttc agtaaaaaaa ccaatatttt ctactggcgt ttcttcctga
     8821 taaatcaagc gtttaaccag gaatttgata tgaaccatta agcgatgata agtcagtgat
     8881 tctttatcat aaacgtttga ataggatttt tctagatggg acaaaatcgt ggtcgtcatt
     8941 tctactattt ttaagctctt taaactatta ggctctccta gctcagcatt aatgatatgc
     9001 atagtgatga atcctacttc atttgaccct aactcggttt gatatcgatg attgattcgc
     9061 tcgaccactt ctactgctat atcatattca tttggataaa ggaatctcat ttctggcaac
     9121 ataattgctg tgacatctct atcttctctt tttcgttgaa tagcaaaata aatatgatct
     9181 gtcaatgtaa tatagatatt tgaatctaat ggagaggaga tcctagctgt tatcagttca
     9241 atcacttctt ccgcaaaatc cataaattcg attggaatct gcgagactat ctcactgaat
     9301 cgtttgatct tttctttaga atctaataca aataatttgt cagcatcctc aacatcaaat
     9361 ggcttactca ctttagcaga aaagccaatc cctttcccca tcgcaatttt ttctattcca
     9421 tcgtcatcga tcaatacagc attatgattc agctttttca tcacatacac cgttaattca
     9481 cctcctctat atgataaaac aaaacaactt gcagactcct tcatacatat ttttgtctcc
     9541 aaacaatttt taaagacaaa aaacgaccta agaaagaacc ctttcttagg tcgtgcctaa
     9601 ttgaatagta gcaccctaca taatttacaa aaatagttta acacataata aaagcgttat
     9661 caatatcttt tttactcgtt ttatgataat acttgtatcg aattgatttg gaggtaatta
     9721 cataaaaaca aaaaaaatat agcaactctc tataagaaag ttgctatatt gataattata
     9781 ccggcggccg gggtcgaacc ggcacgtcct caacggacac tggattttga gtccagcgcg
     9841 tctgccaatt ccgccacgcc ggcaaattta aaaataataa ctggggtagc tggattcgaa
     9901 ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc caagaataaa
     9961 aggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg cgttaaccac
    10021 ttcgccacat tcgccatcat aaaagatgtt atttaatttc tcaataaaaa actaagatga
    10081 agacatctta gtcaataccg gcggccgggg tcgaaccggc acgccctcaa cgggcactgg
    10141 attttgagtc cagcgcgtct gccaattccg ccacgccggc aagttgattt attagtacta
    10201 aggcggtaac cggatttgaa ccggtgatga aggttttgca gacctctgcc ttaccacttg
    10261 gctataccgc cattatacta ttatcaatct ataaaataaa gaataaatta tag
//
LOCUS       DAZKOW010000100        10049 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000100 DAZKOW010000000
VERSION     DAZKOW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.100"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            255..734
                     /locus_tag="ACG912_002380"
     CDS             255..734
                     /locus_tag="ACG912_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFQ0513668.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            767..2995
                     /locus_tag="ACG912_002381"
     CDS             767..2995
                     /locus_tag="ACG912_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0513669.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3124..4767
                     /locus_tag="ACG912_002382"
     CDS             3124..4767
                     /locus_tag="ACG912_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFQ0513670.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4840..5385)
                     /locus_tag="ACG912_002383"
     CDS             complement(4840..5385)
                     /locus_tag="ACG912_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFQ0513671.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5408..5893)
                     /locus_tag="ACG912_002384"
     CDS             complement(5408..5893)
                     /locus_tag="ACG912_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFQ0513672.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5883..6695)
                     /locus_tag="ACG912_002385"
     CDS             complement(5883..6695)
                     /locus_tag="ACG912_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFQ0513673.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6834..8366)
                     /locus_tag="ACG912_002386"
     CDS             complement(6834..8366)
                     /locus_tag="ACG912_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFQ0513674.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8475..9506)
                     /locus_tag="ACG912_002387"
     CDS             complement(8475..9506)
                     /locus_tag="ACG912_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0513675.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3353 a         1852 c         1928 g         2916 t
ORIGIN      
        1 tacaaaaaag atgttggtga agttcaccaa catcaaaaag tatagagcca aaatattcca
       61 acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt
      121 ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc
      181 gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat
      241 gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag
      301 aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa gggctttcac
      361 tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac
      421 atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt
      481 tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat
      541 tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg
      601 tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac
      661 tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg
      721 agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc
      781 actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa
      841 aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca
      901 agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac
      961 acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt
     1021 tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa
     1081 ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact
     1141 gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga
     1201 gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc
     1261 tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc cgactgtcat
     1321 ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa
     1381 tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg
     1441 aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga
     1501 aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat
     1561 ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc
     1621 aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac
     1681 ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc
     1741 cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact
     1801 ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa
     1861 agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga
     1921 aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga
     1981 gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag
     2041 tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat
     2101 gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc
     2161 gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc
     2221 tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga
     2281 atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa
     2341 aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa
     2401 ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga
     2461 tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc
     2521 cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga
     2581 ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca
     2641 atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc
     2701 acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa
     2761 tattagtgac ggtcagccaa aagcgttgcc ggattataca ggcaaaaaag caaaagaaga
     2821 tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat
     2881 tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac
     2941 tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa
     3001 taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa
     3061 caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac
     3121 gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct
     3181 agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt
     3241 aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca
     3301 tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg
     3361 atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg
     3421 gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct
     3481 aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg
     3541 aataattgga catcgatttt cggcggttct gcttgggagt attctcatga aactggacag
     3601 tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg
     3661 aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt
     3721 ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct
     3781 ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt
     3841 tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga
     3901 ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat
     3961 atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca tgcattaagc
     4021 gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat
     4081 gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca
     4141 aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag
     4201 gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg
     4261 aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc
     4321 attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat
     4381 gctggttttt ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat
     4441 gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc
     4501 attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat
     4561 ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac
     4621 ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta
     4681 ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa
     4741 gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt
     4801 gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc
     4861 accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa
     4921 acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg tgtcaccgat
     4981 atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa
     5041 agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa
     5101 attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc
     5161 ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca
     5221 ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct
     5281 gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac
     5341 tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct
     5401 ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg
     5461 caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg
     5521 gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta
     5581 ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc
     5641 cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat
     5701 cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag
     5761 cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga
     5821 tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt
     5881 gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta
     5941 tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct
     6001 actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca
     6061 caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga
     6121 tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc
     6181 acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac
     6241 gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc
     6301 gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct
     6361 tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt
     6421 atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca
     6481 tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata
     6541 gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt
     6601 ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat
     6661 ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt
     6721 ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt
     6781 aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac
     6841 ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc
     6901 gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc
     6961 tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga
     7021 gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa
     7081 aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc
     7141 aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca
     7201 taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac
     7261 agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc
     7321 cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa
     7381 ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga
     7441 agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt
     7501 atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta
     7561 gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga
     7621 gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat
     7681 cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga
     7741 aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc
     7801 catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta
     7861 tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt
     7921 tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga aatttctctc
     7981 actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg atcgataaat
     8041 acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc accaaggtaa
     8101 gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat aataactttg
     8161 tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac actttcgcag
     8221 cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag
     8281 ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa taagttcaaa
     8341 ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa gtaatataca
     8401 aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg tgaaaaaaag
     8461 atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa
     8521 aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta tcgattacca
     8581 cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata acgatatgtt
     8641 tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa aatttggcga
     8701 atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc
     8761 gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc ttttggtatt
     8821 ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt ggtcaaagat
     8881 acatctttac gtttaagttc tactacttca tggatcttta atccgcaata cagaagaatg
     8941 gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc tctgcttgtt
     9001 tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata
     9061 tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga aatctcttct
     9121 tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt
     9181 gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg
     9241 aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg tttttctaaa
     9301 tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata atcagtaaac
     9361 agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga acgattttct
     9421 tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa cttttcttgc
     9481 agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc attttctata
     9541 ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg aagcaaattt
     9601 ttaactaagt actagctaaa agatatgaag taagaagata catttttagt tattttatta
     9661 gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat gtggttactt
     9721 catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc ttttatttcc
     9781 gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca ataatggttt
     9841 gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg gtacttcatt
     9901 actttacatg aagatttatt ctatggtagg agcaattata ttgagaatta atcctttttt
     9961 atataagttg tttgcttatt gatggattgg ataaattaaa ccatcacaac gagtggaaat
    10021 tatatgaaat ttttcataaa aattcatat
//
LOCUS       DAZKOW010000101        10011 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000101 DAZKOW010000000
VERSION     DAZKOW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.101"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..1059
                     /locus_tag="ACG912_002388"
     CDS             100..1059
                     /locus_tag="ACG912_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFQ0513676.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1536..1736
                     /locus_tag="ACG912_002389"
                     /pseudo
     CDS             1536..1736
                     /locus_tag="ACG912_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2448..3053)
                     /locus_tag="ACG912_002390"
     CDS             complement(2448..3053)
                     /locus_tag="ACG912_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0513677.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            3235..3570
                     /locus_tag="ACG912_002391"
                     /pseudo
     CDS             3235..3570
                     /locus_tag="ACG912_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3735..3950
                     /locus_tag="ACG912_002392"
                     /pseudo
     CDS             3735..3950
                     /locus_tag="ACG912_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4088..5059
                     /locus_tag="ACG912_002393"
     CDS             4088..5059
                     /locus_tag="ACG912_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0513678.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            5199..6512
                     /locus_tag="ACG912_002394"
     CDS             5199..6512
                     /locus_tag="ACG912_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFQ0513679.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            6555..7421
                     /locus_tag="ACG912_002395"
     CDS             6555..7421
                     /locus_tag="ACG912_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0513680.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            7418..8266
                     /locus_tag="ACG912_002396"
     CDS             7418..8266
                     /locus_tag="ACG912_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0513681.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            8280..9752
                     /locus_tag="ACG912_002397"
     CDS             8280..9752
                     /locus_tag="ACG912_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFQ0513682.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9769..>10011
                     /locus_tag="ACG912_002398"
     CDS             9769..>10011
                     /locus_tag="ACG912_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0513683.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         3202 a         1697 c         2026 g         3086 t
ORIGIN      
        1 ttattacgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
       61 aaatagttcc gaccaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
      121 acggatgaac ttgtaataat agagtcttat ttcaaaatga atcaatctgt tgctaaaact
      181 gcccattgct tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa
      241 ggaaaatcag ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga
      301 cgtccgcttg ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga
      361 tggacacccg atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc
      421 atttatcgta tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag
      481 cgtaaaccga atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat
      541 gaacgtgaaa aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact
      601 atcgtaggtc tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt
      661 atcatcacat tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa
      721 tggtttgaat ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa
      781 ttttcaaatt ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca
      841 ggaacgccgt ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt
      901 ttattgaaat ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct
      961 aaacgaaata atattcctag aaaatcactg gattatcgaa cacctttgga agtatttttg
     1021 agttacgtaa gtattgatga tctgtctaac ttaatttgac aattaagaca tttaaaaaga
     1081 aaagtgacag ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt
     1141 ctaatactag gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt
     1201 gtcagctatt ctatattaac attgattgct atggttttag gtacttcatt ttataatgct
     1261 tttgtaagta agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca
     1321 agtaattaat atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca
     1381 cattaagaac gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc
     1441 atatttgttt gttatgctat acatagatct gcataattag acttgcgctg cccgttattg
     1501 aagtttcagg cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga
     1561 aacgctggca gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct
     1621 accccactgt tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag
     1681 atatagatac ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag
     1741 aaacaagcga agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca
     1801 actctaagct cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa
     1861 tacatggatg tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag
     1921 ttgagtattg cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag
     1981 atagctccat ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc
     2041 tgtataatag aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg
     2101 ttcactatcg aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt
     2161 taactgtggg aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg
     2221 gggtgactga actaaccgct gatatgagcg atacttggct gtaggaatat ctttttccgg
     2281 aaaaacaaac aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac
     2341 taatgaaaaa gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat
     2401 agaaacgaaa atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc
     2461 gctttcttta ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca
     2521 actggaactc cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga
     2581 tctgttgagt tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc
     2641 aataatactc cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa
     2701 cttagcaatg ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta
     2761 aaatctaaaa actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta
     2821 actgtatttt ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca
     2881 caatcacctt ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta
     2941 ccactggctt tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt
     3001 cgatctaaat tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact
     3061 ccttttaaaa aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata
     3121 gcatttttaa aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat
     3181 gaaattaagc gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag
     3241 ttaaataaag gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc
     3301 aaactttatc aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta
     3361 actaatgcta tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa
     3421 acggaagggt tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat
     3481 cacattaatt ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata
     3541 ctacgtccgt taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca
     3601 ttttcgcacg gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata
     3661 ctaataaatc aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat
     3721 gttccatgta ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact
     3781 tagtcgtggt gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt
     3841 aggctgctgg aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat
     3901 tgacaaagta tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa
     3961 atttttttac gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa
     4021 tttggaatcg tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt
     4081 tcgtgaagtg aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac
     4141 ggtatctaga gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc
     4201 tgcaattagt gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag
     4261 tagcaataca atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc
     4321 ctactatgta gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa
     4381 cggcagtgcg gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg
     4441 aattataggt attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt
     4501 cagtatagac cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg
     4561 tgggcaaatt gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg
     4621 gggtgtttca ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg
     4681 tgaggaaaat aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga
     4741 ggaacaattg ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa
     4801 tgatttcatg gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa
     4861 ggattttcaa attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact
     4921 atcaacgatt gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc
     4981 gatgatttta aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg
     5041 agagacgact aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga
     5101 aagtgatttc aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa
     5161 ataaaacgga aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa
     5221 ttgcagcaat tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa
     5281 gttcaggtag cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc
     5341 ctgaaggaaa agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat
     5401 ataaagctgt tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag
     5461 taaatgctgc aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata
     5521 ctgcagctta tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg
     5581 acatggatga tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg
     5641 cgtttggttt ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg
     5701 ctggaattac agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc
     5761 aagcaatttg ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg
     5821 cgtcgggaga cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg
     5881 atgtcgttaa tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg
     5941 aagcgttgga atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg
     6001 aaaaaggatt tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag
     6061 atttagagga aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg
     6121 caactgggga gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag
     6181 agaaaaagga tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta
     6241 ttatgagttt agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag
     6301 atgaagtgtc ggaaggaatg caggtattaa ttaagcaaaa ccaactttct ggacatgccc
     6361 gcccagtggt tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata
     6421 ttagctactt cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa
     6481 tgcaacaggc aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt
     6541 taaaaaaacc gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc
     6601 tgctgctgac gatttttctg atcatacccg taatcatggt aatttattat gcctttactg
     6661 attattattt gttaacgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac
     6721 tgttcgctga tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga
     6781 ttattccggt ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta
     6841 aaggaaatat cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta
     6901 tttcgatttt atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg
     6961 caaagattgg aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc
     7021 ttgttttcgt atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa
     7081 tgcaaaatat tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc
     7141 agcaatttcg ctatatcacc atgccattat taaagcccac ggcgattttt attttactta
     7201 ccacattgat ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac
     7261 caatgaattc tactatgaca atggtttatt acatttacca aactggattt acagaccgta
     7321 tggtaggcta ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa
     7381 ttgctcaaag aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct
     7441 aatgatcgta gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct
     7501 gtggatggta gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt
     7561 caaagctttt ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat
     7621 ttcccggttc agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc
     7681 ctgtgggtca atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc
     7741 aggcaagaaa attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt
     7801 tttaattccg caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt
     7861 gattttacca gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc
     7921 gattccagaa gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt
     7981 ctttcacatc atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt
     8041 tatctccagc tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt
     8101 ttcaatgcaa gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt
     8161 gatggctgtt ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa
     8221 atatattctg cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa
     8281 tgttgcaaaa acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc
     8341 aagaataccg gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg
     8401 gttttgttta ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca
     8461 tctggggacc aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt
     8521 tgccagtagc actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg
     8581 ccatagaaaa ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc
     8641 aagttcgaca agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg
     8701 ccggaaatcc aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc
     8761 gcgatcccaa agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg
     8821 cagatgctcg tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt
     8881 attccgtatt gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt
     8941 ttgaactaga tggcaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca
     9001 atgcttacca aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag
     9061 gcaaatttct agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc
     9121 agacctgtga aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc
     9181 gtaaaatacc tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag
     9241 agctgagggt ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa
     9301 ttgatccgca ggaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg
     9361 acttagtata tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac
     9421 tagaacttca ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac
     9481 gggagtgctt tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg
     9541 aagtgttgaa aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa
     9601 ttttccgtga tacttcagct attgagcttt ttctaaatga tcgtctgact atgagtacaa
     9661 ctttttatga gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct
     9721 tttcattgga aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt
     9781 tcaatcgaag caggcggcac aaaatttgtg tgtgccatta gcgatgaaga aatgacgatc
     9841 aaggaaagag tgagctttcc gacaacgaca cctgaggaaa caataccttt agtcattgac
     9901 ttcttcaaac aataccaagc agatttagca gggattggga ttggctcatt tgggcctatc
     9961 gatatccatc gcgattccgc cacttatggg tacattacct cgaccccgaa a
//
LOCUS       DAZKOW010000102         9950 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000102 DAZKOW010000000
VERSION     DAZKOW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.102"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(46..1557)
                     /locus_tag="ACG912_002399"
     CDS             complement(46..1557)
                     /locus_tag="ACG912_002399"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFQ0513684.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG912_002400"
     CDS             complement(1879..3231)
                     /gene="murF"
                     /locus_tag="ACG912_002400"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFQ0513685.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(3384..4460)
                     /locus_tag="ACG912_002401"
     CDS             complement(3384..4460)
                     /locus_tag="ACG912_002401"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFQ0513686.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            4656..6401
                     /locus_tag="ACG912_002402"
     CDS             4656..6401
                     /locus_tag="ACG912_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFQ0513687.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(6681..7118)
                     /locus_tag="ACG912_002403"
     CDS             complement(6681..7118)
                     /locus_tag="ACG912_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFQ0513688.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(7102..8475)
                     /locus_tag="ACG912_002404"
     CDS             complement(7102..8475)
                     /locus_tag="ACG912_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513689.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(8797..9285)
                     /locus_tag="ACG912_002405"
     CDS             complement(8797..9285)
                     /locus_tag="ACG912_002405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0513690.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(9307..9909)
                     /locus_tag="ACG912_002406"
     CDS             complement(9307..9909)
                     /locus_tag="ACG912_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0513691.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         2913 a         2119 c         1798 g         3120 t
ORIGIN      
        1 aaaaatgaaa gtttctctta aaaaagagaa actttcattt tatgtttaat cgttatttga
       61 acggattaca aatccgcgtt ttttatcatt gtgacgttta ccgccatctt tttggctgtt
      121 attatttttg ctataaccgc cttttgattt gtttccacga taattaccgc cgtcacgacg
      181 attacggttg tttccgccac cacgtttgcc atttttattg tatccttttt tctgcattgg
      241 caatgggcgt tctggtgtga tcttcactgg cactgcatca gctggatctt ttgctgtcgt
      301 ttttagcaaa agtgcaacta aatcttgtgc agaatattct tctaataatt tatctgctgt
      361 ttgcaggtat ttatctagac cattttctgc aagttttgtt tcgatttgtt caactgcagc
      421 acctaattga cctttgaatg cttctttttc ggttggtgga cgtaaagtcg tcatccgttt
      481 tttcgtcaag ttttcaatca catgcaagta gtccatttca tttggtgtaa cgaaagtcac
      541 agacataccg ccttttccgg cacggcctgt acgtccgata cggtgaacgt agctttctgg
      601 atcttgaggg atatcatagt tgtacacatg agtgacacct gaaatatcta atccacgtgc
      661 agcaacgtct gtcgctacaa ggatatctaa atgaccgctc ttaaatgaac gcaatacgct
      721 cattcgtttt tgttgagaca aatcgccatg gattccttct gcacggtaac cgcgtgcttc
      781 taatccgcgt gccaattcgt ctacacggcg ttttgtacgt ccgaagacga ttgttaattc
      841 aggtgtttga acatcaaaca agcgagtcat gatatcgaat ttttcgtatt cttttgcgcg
      901 gacatagtat tgatcgatca agtcagctgt catttctttc gctttgatct tcacatgatg
      961 tggatttttc atgaatttaa cgccaatatt cttgatagct ggcggcatcg ttgctgagaa
     1021 aagtaatgtt tgtctttgat caggaacttg tgaaatgatt ttttcgatat cttccaagaa
     1081 gcccatattc aacatttcat ctgcttcatc caaaactaat gtttgaacgg ttccaagttt
     1141 caatgtgtgg cggttaatgt ggtcaagcat acgtcctggt gtacccacaa cgatatgtgg
     1201 acgatctttc aagccacgga tctgacggcc aatatctgct ccgccgtaaa cggcttgtac
     1261 acgtactttt ttgtcgcgcc ctaaacgata taattcttct tgtgtttgga tagctaattc
     1321 acgtgttggt gcgataacta aaccttgtaa ttcgtgacga tctggatcga ttttttctaa
     1381 cattggtaat ccaaatgcag ctgttttccc tgtacctgtc tgtgcttgtc caataacgtc
     1441 ttttccagct aatgctagtg gaatcgtttc tgcttggata ggcgttgctt cttcaaaacc
     1501 agcgcgttcg actgatttca acaattctgg tgataattct agttctttaa atttcaaatg
     1561 aatcctccta tatggttgtt aactgaggat gtagcttttt tgctaccccg tcgatttgtt
     1621 cggtcaaagt gtattgtttc tttttgacag tggccacgca cgtttttatc cattcttagc
     1681 ccctgtggca ccaccgaaaa atcactttgc gaagtttagg cattagaaaa gccaaacggg
     1741 tcgcgttcag ctttatcatt attatgacat ctggcttcaa caagttgttt ctatggcaaa
     1801 accagtccaa tttctcttca tctttcggca ataggttatt ctaacatagt ttttcgtttt
     1861 ctgcaagttt acagattttt atttcatttg ctgaagttgt tctaccactt caaataagcc
     1921 catgctgttg ctgcctttta gcataatact atctgttgct tcaagatttt gtttgatagc
     1981 atcaatcaat ttattctttt ctttttcaaa atagaacaca ggtagttggg ggtatttttg
     2041 gttcaattct tcttttagtg catgcatttg ttccccataa aggaaaatca tggcgaattt
     2101 ctcaggatcg atatgttcac tcatttgcgc atgcatattt aaagactctt tccctaattc
     2161 cagcatatct gctaacacag cgattctgcg tccttttgtt ggcagatttt taaaagtatc
     2221 taataccagt cccatggctg ttggatttgc attgtagaca tcgctcaaaa tagctgcacc
     2281 atttcctgcc gttagccatt cggttctgtt ttgagtgata gaaacatggg cgagcccttg
     2341 gcgtatctca ggtatggaca gtccaaagaa gcgcccgatc gtatacgcaa tcaaagcatt
     2401 cgttacatta taacttccag gcagcgggat cgtaaatgtt tctccttcta ctacaaaatc
     2461 ggtttgtgct tttccttttg ctgtcacttc cgcatgaaca tcgccatttc caataccgaa
     2521 tgttgtgact gtttgtacta atgttgccgt taaaggtgtc agcaacggtt catctgcggg
     2581 cacaacgagt aatccgtctg atacaagtcc gtcagtgatt tccattttgg ctttagcgat
     2641 tccttctcgc gacccaagat tttctatgtg tgcttcaccg atcattgtga ttgctgcagc
     2701 ttttggttga gccataagtg aaagttccgt gatttcattg gcgtgatcca tgcccatttc
     2761 caaaacgatc ttttctgtat cttggggcat gtgcaaaatc gtgtaaggaa gaccaatatt
     2821 attgttgtaa tttccttgcg tcttatatgt tttgtatttt tcagacaaga cagacgcggt
     2881 tagatctttc gtggtggttt taccattgct tcctgtaatt gcaacgactt ctgcgcctac
     2941 tttttccaag taataggttg ccaattgctg catggctttc aaaggatctg ctacaggaat
     3001 aactggaaaa ttttttggag cttgttcaac aggcaagctc caaaatgaag cagatgctcc
     3061 gtttgtgatc gcttgctgga taaattcatg tccatctcgc gtaccagcaa gaggaacgaa
     3121 taaacttcct tcggttatct tccggctgtc aaattcgaca ttggtaatct ttgtcagtgc
     3181 agagcattcg ctgcccacgg cttgtgcaat ttcttgtatt gttagattca ttgagatctc
     3241 tcctaaaaaa ggttgtgaaa aaagcgtttg gcctaaacgg cgggttcgca tgactctcac
     3301 ttccaagtaa atttctctat tcgtatttag aagctagaat gaacgcggtt acgcaatcac
     3361 tccagcctct tctttatttc tctttattca tttttttcaa aaaaagattg acgctgatgg
     3421 tatcgattca ttcctaactg gatcagttct tcaatcaaat caccgtattt caagcccata
     3481 ttttcccata agagtgggta catactgaac tccgtaaatc ctggcataga gtttaattca
     3541 ttcaggaata attcattttt atttgtcaaa aagaaatcgc accggctcaa tccgcttcca
     3601 cctaacatcg tgtaagctaa cttcgcgtac tcttgcgctt tttgataaac ttcttctggc
     3661 acttcggctg gaatctgcat ttcgatttta ttattgatat attttgcttc ataatcatag
     3721 aatgctacgt cttttacgac ttcaccaggc aaagtcgtcc gaacgtcttc atttcctaat
     3781 acagcaactt cgatttcgcg cgcttcaatt ccttgttcaa cgattgctcg agaatcatac
     3841 tgataggctg ttgctaaagc attttgcagc tcttctcggt tttctgcttt tgtaatgccg
     3901 acactagaac ccatattcgc cggtttgaca aacatcggat aaagcaaaga accttcacat
     3961 tgatcaaata cttttttagg attttctttc cattgattct taagtactgg tacataagga
     4021 acttgcggca caccagcagc ttgtaaaata tacttggtca tgattttatc catggcacat
     4081 gcactggtca atacgcctgc gccgacataa ggcatattca atgtctctaa gaagccttgg
     4141 atcgttccat cttccccgtt tggcccatgt aaaactggaa aaacgatggc tccttcttct
     4201 ttgatttcgc ccggattgat cacttttcct gtaaagcctt cctctgtctg tccacttggg
     4261 tcccatgaaa gatgcaagac atctttgctg gcaggttttt ctgttagtaa tggacctttg
     4321 acccattgtc cttcttttgt aataaatacg agttgaactt ggtaataatt ataataaatg
     4381 gcatttaaaa ctgaaaatgc ggaaagaatg gacacttcat gctctgcgct gcgtccgcca
     4441 tatagtaaag taatcttcaa aagattgtcc tcctgtattc taaatcgttc agtgatttac
     4501 tctcatattt tagcacaaaa caaaaataaa cgaataactt atttataaaa aaacgagcca
     4561 gcttctaaaa gaacgagcgt catttatttt ttgtcagcta gattatttgt cccgtttttt
     4621 ataaaaagtt atgataaaga aaaggaggga tgatgatggc gcaaacaaat ccacgcagcc
     4681 gcttggcaga aatcattcga gtgttcatca attacaatgt agtgccaaac tttgtacagc
     4741 aaaaaaatcc cgaacaggta aaaaaagcct ttgaagaact aggccccacc ttcatcaaga
     4801 taggacagat gctttctgtt cgagaagatt tgctttcttc tgccttcact caaacattca
     4861 aaacattaca agatagtgta cctagcgaca cattttcaac ggtcaaaaag acgatcgaaa
     4921 cagaactttc tctttcatta tcagatatat tcgatgattt ttcaaaatcg ccttttgcct
     4981 ctgcttctat ggggcaggct catcgtgcca agctgaagaa cggtgacgcg gtagtcgtta
     5041 agatccaaca ccctaacatc gccgaagaga tccgacttga tcttcaacta tttgaacgag
     5101 cgatcccttt gatcaaatac attcctgaaa ctagcgtagt tgatctaaaa ggggtactcc
     5161 aagaagtaaa acgttccttg atcaatgaaa tggacttctt aaaagaaagt caaaatggcg
     5221 aacaattcta tcaaaagaac aatggctgga aagagatccg cagccccaag atttatgacg
     5281 ctttttgttc gaaaaaagtg atcgtgatgg aagaaatgtc tggtaaaaac ttgaatcatt
     5341 taatgaacgc cgaaaacaaa actgaaactt ttatcactgg aattcaaaac aagcagctga
     5401 aacaagaagt tgcaaagctt ttagttgaga actttatgaa acaggtgttt gatgatggtt
     5461 ttttccatgc tgatcctcat ccagggaatc tgctattcca tgtactaaca aaagaagaac
     5521 agacacaagc tagccgaaaa actgaaacag tccatgaaaa agagttcggt tcctttgctt
     5581 ttcgagcctc cacttctgca gaagaccctg ttgcgccata taccatcaac tacatcgatt
     5641 ttggtatgat ggggcatctt tctgcaggac ttaggcaaaa actcacacaa gccgttttag
     5701 cgctgtatac gaaagatgct tatcgaatcg aaaaagcggt ccttcgactt tgtcagcaag
     5761 aaggctcgtt tgatgaaagt cggttccacc aagaactgac aagttttctt gaacagtatt
     5821 atgattctcc tattgatgaa atcaacttgc aagaagtatt cacacatgtc gtgacgattt
     5881 gtcatcaaaa taatctgcag ttcgatcgtg atatcacgct tttgctgaaa gcatttggga
     5941 cattagaagg ggtgatccgc gtcctcgatc ctgaagtttc cctcatggaa gtcgcttcgc
     6001 cttttgctca acattatttt ctcactcatc tggatgtaga agatacattg aaacaaagcg
     6061 gattagactt attggaaggg atgaaggctg cgcctaagat ccctcaacag ctccaccatc
     6121 tattagaaat gtggacttcg ggacaaggaa aagtcaattt ggaattgaaa aagcaagaca
     6181 aattattgag caggatcgaa agtatgatca atcggcttgt tttcggtatg attttagctg
     6241 cattgatcgt agggtcctcc ctgcttgtcc aagcagcacc tgtagaaaac gcagaggtcg
     6301 tttccttatt agggatattt acgtatgcta ttgccgcatt cgtcattatt tttttggcaa
     6361 tcgatgcatt gatccaaatg tataagaaga ggaaaaagta gaaaaaggtt gtgacagaaa
     6421 gaatcagctt caagaaataa gaaggggttt tacataaatt gtttctcaaa tttttgtgaa
     6481 aattcggctt atttttcgaa gaggctactt ctgaaacacc gagaataaaa aatgtgaaaa
     6541 agctgttctg cacccagtaa taagaacaat caaaaaggga acatggaaca catttatcct
     6601 gtttccctgt tccctttttg atgtattcga gcataaataa aataccttcg ttacctacta
     6661 tcttctgtac ttggtaaacc ttagtcaatt gcctttttta acgcttgacg aaaagacata
     6721 tgatctttct ccgtaaaggg ttttggaccc tttgtcctta tgccagcttt tcttaacttt
     6781 ccactatgat aacgttgttc tagaagcgtg ccgatattcg cttcgtcaaa ttcttttcca
     6841 gagtgatgga acactcgcac acccttcggc aatacaagtg aagctaaacc ataatcttgc
     6901 gtgatcaaaa agtcattctt cttaaccata ccgacgattt tataatccgc actatcagct
     6961 ccgcggtcta cgtagacgat ttggacatga ttagcgtatt ctttggtcgt ataatggtcc
     7021 acactggtca cgataaccac gtcaagacca taggattctc ctaattttat gacatcttct
     7081 tttactggag aaccatctcc atcaatgaat agcctcattc tttttcgcct tcttccataa
     7141 atgataaaca gttactaatc cgctcagtaa aagaccaaca ttgatccatt ttgccaagat
     7201 aagcagttgt acactttcta tcggcactga ttcatggttc agcgtgatca ataatagatg
     7261 gattggctct cccatttctt ttgctaaaac aaacaaaagc gtcatcaata aaaactggtg
     7321 gatcacactc cctttatttt gagtagtata cttgacaacg atccggcgat ccattggaat
     7381 agctgtatca gtttgataat attccttatt tagccagctg cctgttacta cgtgccagaa
     7441 actaagcaga aagacaccta tcgcaaaacc atttggaatc aagaggacca ataaagaaga
     7501 actcagtcca agcaattgca tagccaatgg agaccatgtt aaacgtttca aaatccatgg
     7561 tccgactatt tttgctgcaa tcaagcctgc tgcataaggc aaatacataa aaatcgaaag
     7621 tttttctaat ccgtacagta tgccaacata aaaactgcta aataaaaaaa ggaaattccc
     7681 tagcattcca tttagaaaag taaacagatt tagccagctt ggcaacttcc attccttctt
     7741 ctggtgtcct aaaacaaaca cgaacaagat gaacagcagc aagaaaccat aaatagcaag
     7801 atcaaaaagc ctttcatcga ataacaatcg ggcatttctc aaaagaaaca ataagccaaa
     7861 gaatatcaaa aataaaacaa gctctcgcgt agctaccaga tttcttttga tgtctttgaa
     7921 atccagttcg taacgcggat aatgactaac cgtatggtag gccatgacgt aaaaaagtgt
     7981 ataaatagta aagcttgctg caatctgagt cggtgcaggc aactcaatcg cttgatacag
     8041 gagaaatagc cagatagctg caaataagta ctttttccca tccatattga taaaaccttg
     8101 cgtcttttcg tgaaaattga ccgtggtatt agcaggtaca agccatgctg cactcagccc
     8161 tagcaaaaca cttgaaaaat aaaataaagg aaaataaaat acgcctaata ttccaagcaa
     8221 tgaaccagca ccaccaatca gcagagcgat catcagcaac gtgtaactat ctagaccaaa
     8281 tcgtagactg ttgactaaaa atagtccagt catccgaaag gcataaaaca gcgtaaatgg
     8341 caaaatcgtc agccaattat gcgagtctgt tagtcccaaa aataaaagat aaggaataaa
     8401 aataaccgtg ttacttaaga taaagggcgt tgtttgtgta aaattacgcc caaatcgttt
     8461 gagtttttcg aacatgacaa tggctcctta aacagtctta taactaatct gctcttcgat
     8521 tttagcatta aaggggaaat aaaccaattt tacagaatat attcttgtaa aaaaaaatac
     8581 agaaagaagc ataagcttct ttctgcattt ttacggtact tgattactca agcagacaag
     8641 cccttctcca caaaagtctt gcccgacacc cctgcatccg gcaggtgata aagatagaaa
     8701 tcaattatcc tgtgcctcca aaatcaatta ggagctctgt aaaccagagt ccttctcgac
     8761 ccgtcgcata aatttacttt aacacagttc ctatgtttat gcgagagctt ttttcatttt
     8821 ataatcgacc atttgaaatc cttgaccgat atctgaaata agttcttctg taatctcaaa
     8881 tccttcatgc ttataaacct cgattgcttg atgattcccc tgattcactc gcaaaaagat
     8941 ctctctttcc tttgtttgcg cagcaagttc ttcataccag cgaaaaactg aagaagtcaa
     9001 tccttgacca cgtactttat ttagcaaata taatttactt aaataaattt ttccatttat
     9061 ctcttcataa gcagtgtaac cgacagtctt ttcttcgctt tttaataaaa agtaggacac
     9121 accttctgtc agttcttttt gaatctgttt ttttgactga taagttttca gcatgtaatc
     9181 cacttgtttt tgaccaatga tcggcacaaa aacttccggc catacttctt gtatgactgc
     9241 catcagctca tccagttcat tttctgttac ttttattttt tccatacacc cacgtccttt
     9301 tttacattat aaatgacctt gcgcttgatg gaataaaaag agcatgatca tgctatacac
     9361 acccaccgac cacggtttca agaaaatcac aatctgcata aattccctaa aagataattt
     9421 cgtcaaacca gctactagac agatcaagtc gtctggcgca aagggaagca gcaaggtaac
     9481 aactagtact tttttaatat ctttcgtttt tcttgtcagc aattcatcaa atttctgata
     9541 cttttttggc gataaaataa gctggacaaa ggctacgcca tagtaacgga caagcaaaaa
     9601 tccgattgct tccccaatca ctaggccaat acaactatat aaaagcccaa atccattgcc
     9661 aaacatcagc atgcccgcta ctgaggagat ccctcccggc aaaataggaa tgatcacttg
     9721 gatcacttgc aacagaacaa atatcatcac tgcatacttg ccaaattgct ggataaacgc
     9781 ttgcaaagac gtgctcgatc gaaaaatccc atgatgataa gcataaagaa tcaacacaaa
     9841 gaaaaaaaac agaccaataa tgggcatgat ctgaagacat ttttgtaaaa ctgatccttt
     9901 tttagacatc tatattcccc acatcttttt tcatttatta aaactgcctc
//
LOCUS       DAZKOW010000103         9811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000103 DAZKOW010000000
VERSION     DAZKOW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.103"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(34..2154)
                     /locus_tag="ACG912_002407"
     CDS             complement(34..2154)
                     /locus_tag="ACG912_002407"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFQ0513692.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2384..3562
                     /locus_tag="ACG912_002408"
     CDS             2384..3562
                     /locus_tag="ACG912_002408"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFQ0513693.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3578..4336
                     /gene="aroD"
                     /locus_tag="ACG912_002409"
     CDS             3578..4336
                     /gene="aroD"
                     /locus_tag="ACG912_002409"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFQ0513694.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG912_002410"
     CDS             4359..4844
                     /gene="ybaK"
                     /locus_tag="ACG912_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFQ0513695.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4915..6168)
                     /locus_tag="ACG912_002411"
     CDS             complement(4915..6168)
                     /locus_tag="ACG912_002411"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0513696.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG912_002412"
     CDS             6285..7634
                     /gene="gdhA"
                     /locus_tag="ACG912_002412"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFQ0513697.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7746..9092
                     /locus_tag="ACG912_002413"
     CDS             7746..9092
                     /locus_tag="ACG912_002413"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFQ0513698.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9400..9786)
                     /locus_tag="ACG912_002414"
     CDS             complement(9400..9786)
                     /locus_tag="ACG912_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0513699.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3128 a         1782 c         2009 g         2892 t
ORIGIN      
        1 aattttgtcg ctgctccttt taaatttatt ttatcattcg gaagaacttg tggtcgttcc
       61 gtgaagttct tggtacgttt gcgttttata aagatcaaca gttgattgtc cgttgtgttc
      121 actaaagaca ctagttgact tgtcaccaag agatttttca gcttctttta atttctctaa
      181 gccattcttg tagtcataat cactggtatc aattgggtct aatccactat tcgtatagaa
      241 ccgaagaaga tcaccattat tgatttggtc agaaacagct aactgtttat caactttttc
      301 tttcagtgaa tcaatttcat tctgtacttc ctcagtaggt tcagtaatag cttcaccagt
      361 ttcgttacta tagatcgtac caccgtagta agtatatttt ggtgtaacaa aatcaccatc
      421 acggaaagaa acaagctgtt catgatcttt cgagaataga tcttgtccta actgaatata
      481 attttttgta tctacaccta gtaagtgaag taatgtaggc aaggcatcaa cttgtccgcc
      541 gtaagtgtga ttgattccgc cctgtgtttg tccagggata tggatcatat aagggacacg
      601 ctgcatttgt gcattgtcgt agtttgtcca agtagaactt gtttttccaa ctaattcagc
      661 tagattttta tttcttgaat tcgagacacc gtaatggtca ccgtaaagga cgatgactga
      721 attatcatac aagccgcttt ctttcaaata attgaagaat tcttgtacgg cggtatctaa
      781 ataattggct gtcgcaaagt aaccgttgat cgtttcatca tttgtattgg ctaacgggaa
      841 tcctgcttcg tcattggtga attttgaata aggataatgg ttagatacag caatgaattt
      901 tgaataaaat ggttgttgca gacgttccaa gtattgtacg gattgttgga aaaatggttt
      961 atcatgcaac ccgtattgga acgaattgtc atcattaaca tcataataag aagaatcaaa
     1021 gaaataatca tatcctaaat tcttgtatgt ctcgtttcga ttccagaatg taccagcgtt
     1081 cccatggaat accgcactaa tgtaaccttg tgtttgactt aaaatatcag gtgcagcttg
     1141 gaaagtattt tttccgccta attgtgtgaa caaggagcct tgattcaagc caaacaatga
     1201 attttccatc agcgtttccg catcgcttgt ttttcctgct tttacttgat ggaaaaagtt
     1261 gtcaaaactg aacgtgctgt tgctgtggta aagactattg atgaaaggtg taacttcatg
     1321 ctggagacca ttctcatctg tcaatttata atcgatcagg aattgctgga agctttctaa
     1381 atggatataa atcacatttt ttcctctagc gagcccgaac aaatcgtcat ttggtgctgc
     1441 ataatgttct tttacataat tttctacttc tttcatatca ttgggacttg cttctgcccg
     1501 cacttggttt gtctggtaag tttgaacacc gtcataaacc atgaaagcat tcaagccaag
     1561 atatttgacc aaataatcac gagagaatgt tcgagtcagc aattctggac gatcagcttc
     1621 cgccataaaa agatttcctg agaaaatcat aactgctaat gtagagatcg caaaagccat
     1681 tcttgctcgg atcgggcgag ggtccaactt gatttttttt gcaatcagca gaacagtcaa
     1741 gataataaaa tcgatccaat agatcacatc atatgggcga aacagtcgaa tggcactttc
     1801 tcccagacca ctcgctactt tcccagcacc taaaatcgtg ttgatcgtaa tgaagtccgt
     1861 gaattctcga taataagtga cattcgaaaa aacaagcaat gtcaaaagaa aataaatgac
     1921 catcatcgtg atataagcaa ctctagggcg tctgatataa agagcaattc caagtaataa
     1981 caatgtagtt gcaatagggt tgataaacaa gataaagtat tgaatgccac tttcgattcc
     2041 gagatggaaa tcaactaaat aggcaaaaat cgttttcaac caaagtaaaa cgactagtaa
     2101 agaaaaaaat ccgagcctct tattcaaaaa ggcaggtgtt tttatacatt tcaagataat
     2161 cttcctttct gcacttctta atcacagaag tgtcctgaaa ccatgttaca cagctcactt
     2221 attctcattt attttactag tatgacaata tagtgtcaat tgaaaccaga agtttatgct
     2281 gtttcattaa ggttttcttt aagcttcatg aaaaaatgag cagtaagaca agtcctatgt
     2341 tttttgttat acttgggaag agaaagctaa agcaggtgaa ggaatgaaag taaaagtaaa
     2401 aaaaagagca agcaatcgat tgaaaaaagg atatccactg atccaaaaag aagaccttgc
     2461 tcatccccaa aaagaaacca cgcaatgggt ggaattagtt gattcgcaaa accaatttat
     2521 cgcaaaagga tatctaggaa agcagaataa gggaatcggt tgggtattga gccagcagaa
     2581 catcccattt gatcaatctt tttttgaaga attttttgtg aagtcaaaag aaaaaagaac
     2641 tttctttttt tcagatgatt ctactaccgc ttttcgtata ttcaatggag aaggagacgg
     2701 gatcggcggt ctgattgttg attattatgc aggttatgct gttttttctt ggtataacga
     2761 aacgttatat gcacatcgtg aattattgat tcaagctttt cgtgaaatct acccggaaat
     2821 cataggaggc tatgagaaaa ttcgatttga atcagtaaat ctgcctgaga gccagttcat
     2881 ttacggaaaa gaggcaccgg aacccttgat cgtaaaagaa aacggagtaa aatttgccgt
     2941 ttatttaaat gaaggattaa tgactgggat ctttttagac caaaaagaag tacggggatc
     3001 attagcagaa ggtttcgctt ctgggaaaac agttttgaac atgtttagtt ataccggcgc
     3061 cttttctgtt gctgccgcaa tgggaggggc aatcgaaaca acaagtgtcg atctagcaaa
     3121 acgcagttta aaaaaaacac aggaacagtt tgaagtgaat ggtttggatg tcgatcagca
     3181 aaaaatcatt gttatggacg tattcgagta tttcaaatac gccaaaagaa aacaattttt
     3241 atatgacatg attgtattgg atccaccaag ttttgccaga aataagaaaa aagtctttcg
     3301 cgtagcaaaa gattatgggg aactggtcaa ggatagtctg gatattttag cagaagacgg
     3361 gatacttatt gcgtctacaa atgctgcgaa tgtctcgata gaacaattcc aaacaatgat
     3421 tgaagaggaa ttccatgcca aaaatgtttc ttttgaacaa cttgctttat atcgtctgcc
     3481 aaaagatttc cagacgacgg ataccttttt agaaggcaat tatctgaaag tgtttgttta
     3541 tcaaattaaa aataaccatt agaaggagcg atgcagaatg gttcgggtaa agggattgaa
     3601 tataggaagc ggtagaccaa agatttgtgc gcctcttatc ggggccgtcc gagaggaagt
     3661 attacgtgaa gcagttcttg cacgtgaagc aggggccgat ttagtcgaat ggcgagtcga
     3721 tcattatcgt gaagtttttt cttttgatga agtagcagaa acattggttc ttatccatga
     3781 gatgcttgat ggactgccgt tattatttac gtttcgtacc ttagctgaag gcggagaaca
     3841 agacatatcg atccgtcagt atcgcgacct ttatgaacat gtgatacaca caggattagt
     3901 agatatggtc gatatcgagt tgttcaaagt agaaagtcta ggaaaaagac tgatagagga
     3961 aatcaagtct ttaaacattc ctttaattat cagtagtcat gattttaaag aaacaccagc
     4021 agaccctgtc ttgctttacc gattgaatgt tatggaacat tttggagccg atatcggaaa
     4081 actggcagta actcccaata atgaacgaga tgttttgcgg ttgatggagt tgactagacg
     4141 ggcaaatgct tttttctcca tgcctattat tacgatgtcc atgggaaatc ttggtaaaat
     4201 ttctcgtctg gttggcggaa cgacgggttc cgttatgaca tttggttcct taagcagcga
     4261 ttctacttct gctccaggac aattaccggt caaagcattg aagcaaatta ttcaattatt
     4321 aaatgaaccg ttgtagaaca agtttgaggt gatatttttt ggcaaagaaa aaaatagtaa
     4381 aaacgaatgc aatccgtatg gtcgaacaaa aaaagattcc ctatacagag cacgaatatg
     4441 agtgggacga aagccaccta agtgcgtcat cggtcgctga acagcttcca gaaagccaat
     4501 ctcgtatatt taaaacactg gttgctgtgg gcaatgtgac ggggcctctt gttgctgtga
     4561 taccgggaga agccgaattg aatctaaaaa aattagcaaa agtttcaggg aataaaaaag
     4621 tagaaatgct ccatttaaaa gatttggaag caacgaccgg atacatacgt ggtggctgct
     4681 cgcctatagg tatgaaaaaa ctatttccta cttatttaga tcaaattgca gaaagctatg
     4741 aacaaatcat tgtctcggct ggcagaagag ggctacagat ggagttagca cctcaagata
     4801 tctgtgcatt aacgagcgga caatttgcag atataaaaca ataaatcata cgtataaaag
     4861 aaagaaggag gccgggatgt tgtttgtcca gtctccttct ttctttttta tacattatgc
     4921 tctttgcccg gcattcattc ctgctacatg ccctgtagcg aaagccgctg tgatattaaa
     4981 gccgccagta taaccattga catccagtaa ttctcccgca aaatacagac catcaatgat
     5041 tttactctcc aatgttttag gataaatttc ctttagagaa acccctccgc cagttacaaa
     5101 tgatttctca ataggaaaag ttttgttgat tgatagctta aactctttgc atagttgaac
     5161 aaaatcttga atttgttttt gagaggcttg ttgtcctgtc agatgatcca tttcaagccg
     5221 ctcaagaaag aaaactaaca atcgctcagg caatatacca tgccaagcat tgaccaagtt
     5281 ctttttggtt gctttacttt tttctgtcaa tatatgaata agctcttgtt tggtttgtgt
     5341 agggaaacaa tccagagaaa gtgtcactgg ttctcccgtt ttctctaatt ctttattgac
     5401 aaaacttgaa caacgcagtg cagccggacc agaaataccg aaatgggtga aaagtaaatc
     5461 catcgtatgt tcagtcaata cacgaccttt tttatttaaa acgcgtaaag tgatgtcttg
     5521 taaagaaagc ccttgaagtg ttttctcctg aatatacggt tcatccgata tcaaggggga
     5581 ctctgtcgca taaagaggcg tcaccgtgtg acccactcgc ttcacgatct tgtacccatc
     5641 tccagtagaa ccagtagatg gataagtccg tccaccagtt gttaagataa tggcaggagc
     5701 atggaactct tcgaattccg tgcgtatacc gtaaatttta tgttctttgt ggatcaattt
     5761 ctctactcga gtagagaata gaagcgtcac atctagttct ttcagacgat tgagcaatgc
     5821 ttcgataatc gtttttgatt tatttgttac aggaaacatc cgtccatgat cctcttcttt
     5881 taaatgtacg ccttgtgatt caaagaagtt catgatatcg aaattattcc attgcgcaaa
     5941 cgtactatat aaaaactttc catttccagg aatatgtgca atcaaatcat caacaggacg
     6001 attgttcgta acattgcacc gaccaccacc tgtcatcagt agtttttttc ctgctttttt
     6061 gtttttttcg atcaagagta cgcgtgcgcc ttgttcagct gcactgatcg ctgccatcat
     6121 cccgcttgtt ccagcgccga ctacgatcac atcatacagt ttttccattc cctcacctca
     6181 aggaaagtgt agcatatctg aagaaagaaa acagaaaaaa atgttttgag ccaaaagaga
     6241 aaatgagcta aaatgaggga agataagcaa aggagcgtgg aacgatgaca aacgcaacag
     6301 aatatgttca agcaattcaa gaaaagctac atcaaaagga tcaagggcaa gaagaatttt
     6361 tacaagcaat cgatgaattc atgcccacgg ttatgtcttt tttggacact catcccgaat
     6421 atatcgaaaa gaatatctta gaacttttaa cggaaccaga aagagttatt caatttcgag
     6481 tgccttggca agatgatgaa ggaaactggc gagtaaatag agggtaccgt atccaataca
     6541 actcagctat cggcccttat aaaggtggat tgcgcttcca tcctagtgtc aatctaagta
     6601 tcttgaaatt tttggctttt gaacagattt tcaagaatag tttaaccggt ctgccaatcg
     6661 gtggaggaaa aggcggaagt gacttcgatc ctaaaggaaa atctgataat gagatcatgc
     6721 gtttttgtca aagtttcatg ctggaattgt caaaacatat cggcccatcg attgatgttc
     6781 ctgcgggaga tatcggtgta ggagctagag aaatcggcta tttgttcggc gaatataaac
     6841 gattgaaaaa atatgatact ggtgtgttga caggtaaacc tttggatttt tggggaagta
     6901 agatacgtac ggaagctact ggttatggat tagtgtatta tgtcaaacat ctattgaacg
     6961 aagaaaaaga ttcatttaat caaaaaacag tttttgtttc cggaagcggg aatgtagcca
     7021 tttatgcaat cgaaaaagtg caagagctag gcgggaaagt tgttacatgt tcagattcta
     7081 gcgggtacat ctatgatcca gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa
     7141 tcaatagaca acgtttgacg gaatacgcaa accaacgtcc ttctgctatt tatcatgctg
     7201 gagaatctgt ttggacattg aaagaaaaag cggatatcgc tttgccatgt gctactcaaa
     7261 atgaaataga cgaaagcttg gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag
     7321 aaggcgctaa tatgccatgt acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt
     7381 ggtattgtcc aggaaaagct gctaatgctg gaggagtagc agtatctgct ttagaaatga
     7441 gtcagaacgt ccagcgattg acttgggaaa gagagcaagt ggatgagcag ctggatcaaa
     7501 tcatggaaac gatttaccat acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa
     7561 atctgcttct tggagcaaat gttgctggat ttgaaaaagt tgccaaagca atggcagcag
     7621 aaggtttagt ttgaaaaatg aaactttctt ggaaatattg tcagcatttt gtttatttgc
     7681 ttgaaaaata ataaaaaaaa acgtaaagta ttacctgtag agtaaaccta taaggaggag
     7741 aacaaatggc acacattcat tttgactatt caaaagtagc accgtttgtc aacgaacaag
     7801 agttagggta catgcaaagc caagtaacag cagcccacaa cgaattacgg gaaggtactg
     7861 gcgcaggaag cgacttccgc ggctggatcg atttaccaac gaactatgac aaagaagaat
     7921 ttgcacgtat caaagaagca gctaaaaaaa tccaatctga ttctgaagta ttagttgtta
     7981 tcggtatcgg tggttcttat ttaggcgctc gtgcagcgat cgacttcttg aatcacacat
     8041 tcttcaattt attagatagc aacacaagaa aagcaccaca aatcttcttt gccggaaatt
     8101 caatcagctc aacatatatt gctgacttga tcgaagtaat cggtgatcgc gacttttcag
     8161 taaacgttat ttcaaaatct ggaacaacta ctgaaccagc gattgcgttc cgtgtattca
     8221 aagagcttct tgttaagaaa tatggacaag aagaagcaaa caaacgtatc tacgctacaa
     8281 cagataaagc aaaaggtgca gttaaagtag aagcagatgc agaaggttgg gaaactttcg
     8341 tgattcctga tgacatcggc ggacgtttct ctgtattaac tgctgttggt ttgttaccaa
     8401 tcgctgttag tggtgcagat atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag
     8461 catactcaag cgacaaacta gaagaaaacg aagcttacca atacgcagca atgcgcaaca
     8521 ttctttaccg taaaggcaaa gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat
     8581 acttctcaga atggtggaaa caattgtatg gtgaatctga aggaaaagac caaaaaggca
     8641 tctatccttc aagcgcaaac ttctcaactg atttgcactc tttaggacaa ttcatccaag
     8701 aaggacgtcg caatattttt gaaacagttg tgaaggttga aaaacctcgt aaatcaatca
     8761 caattcctga acaagcagaa gatttagacg gtctaggcta cttgcaagga aaagaagtgg
     8821 acttcgtaaa tacaaaagca tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc
     8881 ctaacttgct agtcaaaatt ccagaaatgg atgcttatac attgggttat acaatgtact
     8941 tctttgaaat tgctgtaggt atttcaggtt acttgaatgg aatcaaccca ttcgaccaac
     9001 caggtgtaga agcttacaag aaaaacatgt tcgcacttct aggcaaacca ggctttgaag
     9061 atcttgctaa agaattgaac gaacgcctgt aagataaggg tttttcgcta gatttaaata
     9121 atcagtgata gaaacatgtt cgctttaaaa tgaaaactgg tacccattta ggtactcaaa
     9181 catactgcat cgtttattca gaataagggg ctgtgacaag acgtttgtca cagtcccttg
     9241 tttgtataga aatcgattga ttgccttgct ctttagtttg ttttaggact ttaagaacag
     9301 tgaggctgac ttcgaaggtt aattctgttt ttttgttttc tagaatcagt aagcaaaaaa
     9361 gcactcactt cgagtgcttt tttgcgatat attcttagct taagataggc ctaatttttc
     9421 ttttacagat gagggtacct tatcgctggt aattactttt ttctctatga tccgcttttc
     9481 gcttgctttc aactgcaaga tcgttccttc ggggtaagca gaaccattgg cagagtcacc
     9541 agaagtaatg acatcgaaat ctaactgtct tttttcaccg ttttcagcat aagcatctac
     9601 gttgtattta aaacctcttc caattacttc cccattatct gctttgatat cagtttctgc
     9661 aggcattgga gctttgacta ccgcataata atctttacct acataagtag actgatagta
     9721 gtcccaacct ttccatccta taaaagcaat caatatgata acgatcaacc ctaaaatttt
     9781 tttcatgaaa gatctccttt tttattttta t
//
LOCUS       DAZKOW010000104         9724 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000104 DAZKOW010000000
VERSION     DAZKOW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.104"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            33..656
                     /locus_tag="ACG912_002415"
     CDS             33..656
                     /locus_tag="ACG912_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513700.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /gene="trpX"
                     /locus_tag="ACG912_002416"
     CDS             1106..2155
                     /gene="trpX"
                     /locus_tag="ACG912_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0513701.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="ACG912_002417"
     CDS             2152..3033
                     /locus_tag="ACG912_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513702.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="ACG912_002418"
     CDS             3036..3806
                     /locus_tag="ACG912_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513703.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="ACG912_002419"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="ACG912_002419"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFQ0513704.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="ACG912_002420"
     CDS             5117..6280
                     /locus_tag="ACG912_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFQ0513705.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="ACG912_002421"
     CDS             6397..6807
                     /locus_tag="ACG912_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFQ0513706.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="ACG912_002422"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="ACG912_002422"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFQ0513707.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="ACG912_002423"
     CDS             9380..9664
                     /locus_tag="ACG912_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513708.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3372 a         1648 c         2088 g         2616 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgt
//
LOCUS       DAZKOW010000105         9691 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000105 DAZKOW010000000
VERSION     DAZKOW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.105"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..536
                     /locus_tag="ACG912_002424"
     CDS             <1..536
                     /locus_tag="ACG912_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0513709.1"
                     /translation="DYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGS
                     QWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPD
                     LPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDK
                     LLNRIDKEILKVGNIDE"
     gene            529..1956
                     /locus_tag="ACG912_002425"
     CDS             529..1956
                     /locus_tag="ACG912_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFQ0513710.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            1958..3028
                     /locus_tag="ACG912_002426"
     CDS             1958..3028
                     /locus_tag="ACG912_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialyltransferase family
                     glycosyltransferase"
                     /protein_id="HFQ0513711.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            3015..4436
                     /locus_tag="ACG912_002427"
     CDS             3015..4436
                     /locus_tag="ACG912_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0513712.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            4449..5456
                     /locus_tag="ACG912_002428"
     CDS             4449..5456
                     /locus_tag="ACG912_002428"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFQ0513713.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            5468..6472
                     /gene="neuB"
                     /locus_tag="ACG912_002429"
     CDS             5468..6472
                     /gene="neuB"
                     /locus_tag="ACG912_002429"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFQ0513714.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            6469..7617
                     /gene="neuC"
                     /locus_tag="ACG912_002430"
     CDS             6469..7617
                     /gene="neuC"
                     /locus_tag="ACG912_002430"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFQ0513715.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            7590..8267
                     /locus_tag="ACG912_002431"
     CDS             7590..8267
                     /locus_tag="ACG912_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFQ0513716.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            8218..9399
                     /locus_tag="ACG912_002432"
     CDS             8218..9399
                     /locus_tag="ACG912_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFQ0513717.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            9412..>9691
                     /locus_tag="ACG912_002433"
     CDS             9412..>9691
                     /locus_tag="ACG912_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0513718.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEIN"
BASE COUNT         3471 a         1216 c         1724 g         3280 t
ORIGIN      
        1 atgattatcc tagaattaat aggaataaca caactgcatt taaaattttt agagctataa
       61 ttaatcgttg tctgaggatg attaaacttc ccccaaaaat tagacaccat aaatttgatt
      121 tgtatcatgg ttctcaatgg tgggctttgt cgaaagaatg cctaacagaa ttaatacaaa
      181 tgtatgaaca aaatcaacaa gactacctga attttaaaat aggtatgttt gccccagatg
      241 aaaaattttt tcatacactt ttttttaatt cctcgttcaa aaataaaaat gttattggag
      301 gaccagattt accacttgaa ttgaaaaata ttgaagaaac tactttacaa acttcgaaat
      361 tagcaaatat tcatattatt gatccttcca tgaacaaatg gtttgatgaa agggatatag
      421 atcaaataaa agaaagtgaa aaattatttg taagaaaagt taaatcggat tactctgata
      481 aactattaaa tagaattgat aaagaaattt tgaaagttgg aaatatagat gaatagaata
      541 gtacctgttt tagagaagag tttattaagt cgagatgcgt ttcgagtaat aaatttaatc
      601 atttgtctgc tttattttat gcctagtact tttaatgtaa ctaatatccc gatgaaaata
      661 gcttttgctt ggggtggaat tatagttttg tatgaggttt ttataaagaa aaattttttt
      721 aaaatgcgtt tttcttggct actgattgga gcaatgatta gttttatctt aactataatc
      781 attaacagag aaaatttttt aattccatct atttataatt ttggatattt gctgattact
      841 ttgatagtaa tttttcctgc agacttttca caaagtgaag aggaaaaaaa gaaaaaaatg
      901 gtgtatttta ataatgtttt cattatcata atttttttag ctgcgctaat atctatctat
      961 caatttattt ttttgatttc ttatcatgtc ccaacaggaa cgccaggatt acttgcaaga
     1021 caaggtttta ttgaacatcg gttatttggt gtctatacaa gtccaaatgt tggagctatg
     1081 tttggatata cttctattat tttaagttta attagtgtat taatttcaaa aataacaaaa
     1141 acgattcgtg ttttttatat gatgaatttt atcgtacaaa tattttatat tacactttct
     1201 tcgtctagag gtgcccaggt agtaatttgt tcttttttag tattatttct tttgttgttt
     1261 gccaattcaa aatttagaaa gagcattaga gaagtactac ctgtaaaaat caaatggatg
     1321 gtaagcatat tattagttct attggtatat ttttcttttg gtactaatta tgctaaggat
     1381 tttttagcaa caattcctgt atcgattgat agaaaagtag aagttataga gtcaaatgaa
     1441 tcagataaaa ctccacttcc agaaaaagaa gtaatattac agcattctag tgataactcc
     1501 gaaatttcag ctggtcgttt tacgatttgg aaagctgcgc ttaaagttat aagaccaact
     1561 ttgttatttg gttatggaga gaccgatttc tatgggaata gcgatgcaaa atttttgaaa
     1621 aaagtagatc ttgatactac agatgtgaat gaactaaaaa gagcacatgg aaacatgcat
     1681 aatggatttc ttcaagtact tgtatcaagt ggtataattg cttttatatg tatctatgct
     1741 ttctatgtgt taaatatctt tagtttactc aaaacatttt ttagaagtaa agtgaattat
     1801 gcgtttcttg gtattgtttt aatttttata ctatctattt ttatagatga aatggtggag
     1861 gctcatgtac ttttcaataa gcgcgatgtc atttcaattg ttttctggta ttatttggga
     1921 agcatttctt acttgtattt aaaaaaggag aattaatatg aagacagctt tcattgcttg
     1981 gactccttta catttgatta atatattaaa tgtttatcaa aatttttacc gagaatcaaa
     2041 aggagattta tttatttatt ctgaatttga aaacgctaga agatatagtg agcgtataaa
     2101 agaattaggg ctatttaatg atgttgtttt tgtgaatcac cggaaaatcg gttcttcctt
     2161 taaacgtaaa attaacttgt taactaacca taatcaattt ttctcggatg atttatccgt
     2221 ttatgatgaa atttttatac agggggaaaa ttatttcaca aaaatacttt tctccaatgt
     2281 ttacaaaaaa aatccagatg taaaactaaa ttatatagaa gatggtttgg gtacttattt
     2341 gaagaagagt atattttcaa caagtaaaaa cgttcaaaga gtttttaaaa tattaaataa
     2401 aaatagcatc ttcaaaaaaa aaattgacga ttattttgtc tattgtccgg aattgttact
     2461 ttatgatgta aaaaatataa aaaaaattcc caaaatagat gaaaacagta aattgatgaa
     2521 aaacatacaa tcaatctttg ggatagaaga agtttttgtc ggagataaat gtatcttttt
     2581 tgatcaacct ttattatctg atggttatgg catagatgaa acagatattt tcgctaaagt
     2641 aaatagtata ttttctggaa aaaaagaaac tttaattaag cttcatccta gagcaaggac
     2701 aaatcgttat ggtgaaaaaa acatattgaa ttccagttta ccgtttgaag tcttgctact
     2761 tgaaaatgac ttctcttcaa cacttttgat tagtcctata tctacaattt ctttttcacc
     2821 atctatgatg tttgaaaaga cgatcaactc tattatgttg gctaaattaa ttttaaatga
     2881 atatacagca ttatctgaag ataaaaaaga aacattgaca aatattgttc ttttctgtga
     2941 acattataac cgtctatcag aagaaaaaat tttactccca gaaaactggg cagaattaag
     3001 aaagttggta gagtatggga ttatttaaca cactttccaa gtcaaaatta gttaagtcca
     3061 gtttctggta tactataggg aattttttta ttaagggaat ttcattcata actattccta
     3121 tttttgtaaa tattactact caagcagaat atggtttgat taataatttc acttcatttg
     3181 tatcaatttt ttcgattctt gttgggctaa gcctaaattc ggcaattaat aatgcaagtt
     3241 ttgactataa agatgacata aatgaattta tgtcatcggt actttctcta gctacacttt
     3301 cgttaattgt gttcttatta atagggaaca tatatttttt gttttcaaat gctttttttg
     3361 atatgaacca acttgtattt aatttgatgt tattacaaag ttttgctagt tttgtaatta
     3421 gttatatcca gtcttatttt acgattaact ttaaccattt taaatatttg attttatctt
     3481 ttatatctac ttttttgaat attgcaatct ctgtagcttt gatgcagaca ttatattttg
     3541 atgataaata tgttggcaga gtagtcggcg gaacactgtc atttgtgttg ataggtttag
     3601 taatttattt aagaataatg ataaagggtg ctcatttttt tgataaaaaa cagtggagat
     3661 acgctttaaa aatctctttg ccgctaatcc cacattcctt agcaaatata gttttagcac
     3721 aattcgatag aattatgatt aattcttatt taggtcctgc tgcgggggga atatattcct
     3781 acattagtaa cataggtatt attctaagcg taatatggat gtctactaat aacgcctggg
     3841 ttccatggtt ctatggtgag atgagtaaaa ataatgaaaa aaaagtgaag aaagttagta
     3901 actattattt agtagtcttt actttgatta cgatgattgt aatggtagtt tcgattgatt
     3961 tagcaaaatt tatggctccg aaagactatt attctgggtt atctttagtg gttccgataa
     4021 ccttaggcta tttctttcag tttttatata gtttaccagt taatgctgaa ttttatgaga
     4081 aaaaaacatc gtttattgcg attggtacag tagcaagtgc tatagttaat atactattaa
     4141 acatgatttt catacctagg ttaggaataa ttgctgctgg atacacaacc gtatttacgt
     4201 attttttact ttttattttt cattataata tcgctaaaaa aattacgaat aaacaattat
     4261 ttgacacaaa aaaaatatgt atcataataa tttttgtatc aattttttct ttgatacttc
     4321 atattttaat caatcaaatg ttgattcgtt acgttttgac atcattattg ataattagtt
     4381 tagttatata ttttttaaag attaagaatc gaattataga tgactataat tcataggagg
     4441 agatataagt gtttaaagga aaaacaatat tagtaactgg cggaactggt tccattggaa
     4501 gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt tatagtagag
     4561 gtgaagaaaa acaattttat atgcaacaag agctctcaaa tcacactaat ttgagatttt
     4621 taattggtga tatcagagac tctcaaagga ttgattatgc atgtcgtgat gtggatatta
     4681 tttttcatgc tgccgcaatg aaacacgttc cagcaagtga atataatcct atggaagctg
     4741 taaaaacaaa tgtgatggga actcaaaatg taatagatgc agcgatcaaa aataatgtaa
     4801 aacattttgt aggtatcagt acagataaag tagtgagtcc tactaacacc atgggtgcga
     4861 caaagttatt gagtgaaaaa ttaataatag ctgccaatca gtataaagga aaaacagaaa
     4921 caatattttc ttgtgtaagg tttggaaatg tcatggggtc aagtggatct gttattcctc
     4981 tttttagtaa acaaataaaa aacaataaag atattacttt gacagatgtt gatatgacac
     5041 gatttatgat gtcaataccg gatgctgcaa gattagttct gtctgctgct atgaattcaa
     5101 aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa tattttaact ttagctcaat
     5161 cattaataaa aatatataat gatacacatg atgaaaaata tagaggtgaa ataaaagttg
     5221 taggtatcag gccaggagaa aaattatatg aagagctaat gactacagac gaaattgaac
     5281 gctcatatga aaatgataaa atgtatatca ttccaccaca ctatcatgct actgacgaac
     5341 tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc ttcagatgca acagaattaa
     5401 tgagtggaca ggaatttatt agaatcgcaa aagagaataa tgtcgtgact tggtaaggag
     5461 ttttatgatg aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc ataatggaag
     5521 tctagattta gctttagaaa gtattgataa agctaaagaa tgtggagcta acgcggttaa
     5581 atttcaaaca tttaaaacga ataagctagt ttccaaaaag gctccaatgg caaaatatca
     5641 gaaggataat cttaaagagg acagttctca atttgaaatg ctaaaaaaat tagaattatc
     5701 tcaatctgat tttattagaa taaaagatta ttgtatacaa aataaaattg attttttgtc
     5761 tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac aaggatttaa
     5821 aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata tgtatgggtt
     5881 gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg ctgtagattg
     5941 tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg ctagttctga
     6001 agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac ccgttgggta
     6061 ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg gtgctaaggt
     6121 aattgagaaa cattttacct tggataagga actacctggt ccagatcata aagcttcttt
     6181 agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa tatttttagg
     6241 tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat caagaaaaag
     6301 cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat ctcttacaat
     6361 aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg gcaaaaaagt
     6421 taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat gaaaaaaata
     6481 cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc tgagaattta
     6541 ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc ccataaatat
     6601 ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga agttcaaagt
     6661 ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga gataaatggt
     6721 ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg ggatagagga
     6781 gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc acatattcac
     6841 ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac gaaaatagca
     6901 tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat gggggaagat
     6961 tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa taatagatta
     7021 cctagtttta gagatataaa agaaaaatat catctaaaat ttgaaaagaa aaaatacgtt
     7081 ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca aattttagct
     7141 ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc taatagtgat
     7201 gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga tattatttat
     7261 attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa atttatgatc
     7321 ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt aataaattta
     7381 ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa tctaaatgaa
     7441 aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa aagcttgaaa
     7501 aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa aacattgaat
     7561 caaatattga atgatgggga agatattaag tggattcaaa aacaaatagc ttattaaaat
     7621 atactagtga tccatacatt attattgggg ctggcggaca cgcaaaagtc attattgaaa
     7681 ccttaagatt agaaaataag ttaatatatg gattttttga tgataagcaa aataaaatgc
     7741 aagatctgaa atatcttggg aaaataaacg aattgaatag ctgggcggat agcaaaaaaa
     7801 aaatctataa atttattgta gctattggtg actgtgtagt aagaaaaaag attataaaaa
     7861 aattgaatat gccaagtgag caatatggaa tagctgttca tccaagtgct atagtggcaa
     7921 aagaaatttc aattggttat ggaactgtta tttttgcgaa tgctgtaatt aacccagatg
     7981 ctgtaattgg agagcatgct ataataaaca caggtagcat tgttgagcat gattgtagaa
     8041 ttaataacta tgttcattta tctccaggtg tatgcttagc gggtggagta catgtaggtg
     8101 taggaactca aataggaatt ggttctcaat gtatccagat gaagaaaatt ggatcttggt
     8161 gtatgatagg cgcagggagc actattgtta aggatattcc ctcacattcc ttagcttatg
     8221 gcaatccagc taaaattaag aaagaaggaa ttaattttga attctagaat tttattatca
     8281 agtcctcata tgggaggaac agaagaaaaa tatgttgcta aggcttttaa agataattgg
     8341 atagcgccat taggtgaaaa tgtggataaa tttgaagaaa gtattacaaa atatactggt
     8401 tctaagggcg gagcttctgc gttaagttca ggtacagctg ccatacatct tgccttaaga
     8461 ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt catttacttt tatagctaca
     8521 gtaaatccta ttttatatca aggggctgta ccagtgttca ttgactcaga acctaataca
     8581 tggaatatga gccctgttgc actagagagc gcattagaag aagctgaaag aaacaaccaa
     8641 ttacctaagg caatcatcgt agtgaattta tatggtcaaa tggctgatta tgaacgaatt
     8701 ttagctattg caaaccaatt taatgttcca gtgatagaag atgcagcgga atcgttaggt
     8761 tctacttata agggaatttc gtcaggctta tttgggacaa ttgggatcta ttcatttaac
     8821 ggtaataaga tcatcacaac ctcaggtgga ggagctctag tgtctagtga taaaaaatat
     8881 gttgagcatt caagattttt agcaacacaa gcaagagatc aagcaccgta ttatcaacat
     8941 agcgaaatag gttataatta ccgtctaagc aatatttctg ccggtatcgg acgtggacaa
     9001 atggaagtat tgaatgatag agtagctaaa cgaagagaaa ttttcaatcg atataaggaa
     9061 gctttttctg gaattgatag aattgaaatg atgccagaag tggatggcat ggtgccaaat
     9121 cgttggttgt ctactttaac gatcaatgat tataattcta ttgatcaagt agttaacgta
     9181 ataaataaac taaatgaaaa aaacattgaa acaagagctt tgtggaaacc actacatttg
     9241 caaccagtat ttcaaggggt aaaaatgtat taccatgata ataatcttag tgtgtcagaa
     9301 aagttattta atagcggctt atgcttacct tctggaagta atatgacaga tagtgaacaa
     9361 gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg tgtgatattt tatgaacata
     9421 ggaattattg gtttaggttc catggggaaa agaagactta gactcattca agaacacttt
     9481 aatgatattt tactatgtgg tgttgatact aatgaggcga gacgaaagga agttgaaaag
     9541 gaatttggaa taccaactta taatagtcta acagaggcaa aaaatttgtt tgaaataaat
     9601 tcagtttttg tttgtacatc ccctataacc catgaattta ttataaaaga ggctttaaat
     9661 ttagattgta atatctttac ggaaatcaat t
//
LOCUS       DAZKOW010000106         9644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000106 DAZKOW010000000
VERSION     DAZKOW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.106"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG912_002434"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="ACG912_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0513719.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="ACG912_002435"
     CDS             1995..4661
                     /locus_tag="ACG912_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFQ0513720.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="ACG912_002436"
     CDS             5025..5777
                     /locus_tag="ACG912_002436"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0513721.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="ACG912_002437"
     CDS             complement(5799..6953)
                     /locus_tag="ACG912_002437"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFQ0513722.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="ACG912_002438"
     CDS             7138..9579
                     /locus_tag="ACG912_002438"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFQ0513723.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3111 a         1736 c         2066 g         2731 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact acccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agctgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tttt
//
LOCUS       DAZKOW010000107         9593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000107 DAZKOW010000000
VERSION     DAZKOW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.107"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..641)
                     /locus_tag="ACG912_002439"
     CDS             complement(<1..641)
                     /locus_tag="ACG912_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFQ0513724.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQT"
     gene            complement(643..1407)
                     /locus_tag="ACG912_002440"
     CDS             complement(643..1407)
                     /locus_tag="ACG912_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFQ0513725.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(1415..2092)
                     /locus_tag="ACG912_002441"
     CDS             complement(1415..2092)
                     /locus_tag="ACG912_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFQ0513726.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(2129..3514)
                     /locus_tag="ACG912_002442"
     CDS             complement(2129..3514)
                     /locus_tag="ACG912_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFQ0513727.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(3724..3808)
                     /gene="ffs"
                     /locus_tag="ACG912_002443"
     ncRNA           complement(3724..3808)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACG912_002443"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG912_002444"
     CDS             complement(3831..4346)
                     /gene="tadA"
                     /locus_tag="ACG912_002444"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFQ0513728.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            4453..5310
                     /locus_tag="ACG912_002445"
     CDS             4453..5310
                     /locus_tag="ACG912_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513729.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(5391..5726)
                     /locus_tag="ACG912_002446"
     CDS             complement(5391..5726)
                     /locus_tag="ACG912_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513730.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(5793..7163)
                     /locus_tag="ACG912_002447"
     CDS             complement(5793..7163)
                     /locus_tag="ACG912_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFQ0513731.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(7241..7777)
                     /locus_tag="ACG912_002448"
     CDS             complement(7241..7777)
                     /locus_tag="ACG912_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0513732.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            8086..9567
                     /locus_tag="ACG912_002449"
     CDS             8086..9567
                     /locus_tag="ACG912_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513733.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         2908 a         1969 c         1502 g         3214 t
ORIGIN      
        1 tttgtttgtt tcaagctatt ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat
       61 aatacaaaaa ataagataaa gctatgcatg aaaaattctt ctggaagtac attgatgatt
      121 ggatcagtaa cgggatcgaa taaccagtca tcgttgttga aaaacgtttc atgaaaaagg
      181 ataaaaaagc gatcaaatcc tatcatcatg aaaaaaccga aaacaactgg cagtaacatg
      241 ccgatttgaa acggacgaat caaccgccaa agccgatcat tcttttgcaa atatttgata
      301 aacaaaatac tcgggatcaa agtgatgaaa aacacagcat aatccactaa aaacaaaatt
      361 ttcacttcat aaaagtgatg ggctccgcta gcagaaactg ggaaatctgg caagctgagg
      421 atggttttga agggattatt gagataattc attaggtgat caaaattttt cagtaacgtc
      481 tcttgatcta aagaagtata atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt
      541 ggtcgaaagt tgatcgtcac accaactgcc agactgatca gtgtcagaaa taggctgaac
      601 aatccagcat attccatcca ctgccatctc gaatttttca tattagaagc tccattcatc
      661 cagtgattgt ttcacatagg tagctgggat aggcaaccca ggtacatctt cttcttttgt
      721 aaaacctgat aaaacgagca aagtatctat attattgcgt atcccagctt ggatatctgt
      781 ttcatagtta tctccaacca tgattacttc ttctttttgc agccctaata cttcaacggc
      841 tttatccata atgatggctt ctggttttcc gatgtagatc ggcggcgttt gggttgctgt
      901 ctctacaaaa gagatgactg accctgctcc aggaagaagg ccgcgttcag taggtatatt
      961 cttatctggg ttcgttccaa tgaaagtagc acctttttga atagcaagtg ttgctttaac
     1021 tactttttca taggtcagat aattatctaa gcctaccact acatagtcag gagcctcttc
     1081 ttcccatgta aatccggcag ctaaaatcaa gtcaatcaat cctgcttctc cgataacata
     1141 gacttttttc ccttttccat ctgctttcat aaaatcgatt gttgccaaag tagctgtgta
     1201 gactgtctct ggagctacat gaatgtcaaa ctcatctgct agacgattag ctactgtttc
     1261 tggtgactta gtcgtgttat tggtaacaaa caagaaaggt aattttcttt tttgcaattc
     1321 ttccacaaat cgtttaccgg ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa
     1381 atcaattaaa tatcctttat agtccattca tccactactc ttcccttttt ctgatcgtaa
     1441 atgctgtttt tgttttttct acttgtttag gttgttcttg tttttttact ttttcagagg
     1501 tttttgctga ctctgttttt cgatttttaa taaccggttt tttgcgatta gatgcgactg
     1561 gtcctttttt ctcatcgata tacgcacgat tatggttttt tttctttttt cggcgctgag
     1621 ttgtcttttc ttttttaccg ccgattctct cgatgacaaa atacgcacaa ccaaaattgc
     1681 aatattcgta aagatagtct tccaaggaat cgattcgctg atctggatgc gcccgtcgat
     1741 tatccgcttg gaaaaatcct tttaggcgta gttgttcata accccaatct cctacgatat
     1801 aatcatatcg ggctagtaca tcactgtaac gttctccaag cttttctgca ttaaatcctt
     1861 cacgataatc ggacacaagc cgataatgtc tttcacctat caaaaaatta ctgtcatcta
     1921 acagtgtcac tagctcgcct ttgacttttt ctgctttagg ctcctcaacg atttcttcca
     1981 aagcagctgt cagttcttcg gtgactgttg tttcctgttc agctgatgat tttttcttag
     2041 caatttctgt tttatgctca acgttctgct gttcattttg ttttgcagac atgcgttcac
     2101 cacctatctt ttttattata ccttgttttt atttgattgg gtagtgagct ttcaagtaat
     2161 ctcctaccac acttcggata aattcaggga acaaaaattc attttttcca gcaatctcaa
     2221 tcgttggaaa aaatggaaca aacatcaaat gatctacggt agtaaaagta taatttttat
     2281 tcagatcgat tggcttgttc ttccaaaaga cttgatggtt aatagaatca tatgtgattc
     2341 cattatagac catctgccca aaaatcttac cgcgaaatcc cattcccttc ataggatagt
     2401 tacgtaaaaa gttacgattt ttttctattt ctaaaactaa gcggatcaga tcatttccac
     2461 ccaatgtgac atttaacaag tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat
     2521 tgacccgccc ttttggaatt tcagttaaaa acagaccact gtttaatatt gctgcttccg
     2581 tctttcctcg ttctttcacc gcctcaaagg ctacttggat aaacgaatgc tcattgaaga
     2641 tatctagact cagagaaaat ggcaaatctg ctacttcttt ttcttttaat aattcatgcc
     2701 cacgttctag ataactattt atttcttgtt catcttctgt aaaagctgtc attgtttcgg
     2761 tagggatggc tttggctgaa tgcttaagga tttttttctc ttcatcgatg gttaaatgga
     2821 cttcaccgac atactgcccg tactttccag cggctgctat ttggacatca ttgattacgt
     2881 gaccttctct gaataaatga tgggtgtgcg atcctaaaat aacatcaatt gctggtattt
     2941 cttttgcaat ttggaaatca tcctgaatcc cgagatgact catcaaaact aaaacatcca
     3001 cttgtgaacg taaatcttcg actaattctg gcaaaatatc ataaggattc cgtatgtccc
     3061 agccattcgg attataagta agcggaaatg gagcagtaaa tgcaattagt ccgattttcg
     3121 tctggtgttt cgttgtaatg attttatatg gtttcgtcca tgccggtggc tggagcgtat
     3181 ttttatcgaa taagttatcc aggatgatat caaactcagc atgatcgtat aaatgattca
     3241 actcttcttt ggaatttcct acgccttcat tgtttcctat cgtcgctgca tcataaccaa
     3301 cttcattcat cagcaagata ttttcctgtc cattcgttgc ttctgacaat ggatgccaac
     3361 gatccacaaa atcacctaga tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt
     3421 ctcgtttacg ttgatccaaa aaccttctga tccttggcca attttctaaa tgagaatgca
     3481 gatcattcgt atgtaaaatc actatttttt ccatgtaccc tctcgcctct ctttatcttt
     3541 atcataccat aggcagcttt ttcttccatt gaaacaagct ctatttcaga gtttttcctg
     3601 ccttgaagca aattgtataa gtacaatatt cgataaatca gtctggctaa aaaatcagga
     3661 acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt
     3721 tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca gacctgacac
     3781 gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc ttatttttca
     3841 ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg gaaaaagtca
     3901 gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc ttggtggtta
     3961 aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt tggatcatac
     4021 gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca cataggacaa
     4081 ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac tttttcacag
     4141 gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg
     4201 ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat cggaacttca
     4261 tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt ttctttttct
     4321 actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt tcttgtttta
     4381 ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa ataaatacaa
     4441 gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc acagaaaaaa
     4501 aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg
     4561 atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt attccctgta
     4621 atcaacgatc aaaaaaaaca cccatggtat cgctatcttt ttaaagatgc ttcttgtgtt
     4681 caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga attcttaaaa
     4741 aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt ctttttttat
     4801 acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga tgctactgaa
     4861 atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc tgtattcgtt
     4921 ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga aagaaaaatt
     4981 tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat gcaagatgtg
     5041 gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa aagttacata
     5101 gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa tgaattaggc
     5161 aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa atatcgaatc
     5221 gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt actgttttgc
     5281 tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt gacaataatt
     5341 ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc ttacatcatt
     5401 ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt gattgaatca
     5461 ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc catcaaagaa
     5521 tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt tccatcaaat
     5581 ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat cttttccatt
     5641 acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac gcttaatccg
     5701 attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat ctccatcata
     5761 gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc tttagggaaa
     5821 aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt atctaatact
     5881 agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc
     5941 cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt agcaagcgct
     6001 aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt
     6061 acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag atggtcgttg
     6121 aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc cgtccagagt
     6181 ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt ttctttatgc
     6241 atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc ctgatcttta
     6301 tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc agaacgtcct
     6361 gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt ttctacaagc
     6421 cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata aatcagttgt
     6481 cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg tttttgacag
     6541 tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg gaacattcct
     6601 tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca
     6661 agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg ttctatattt
     6721 tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa ccctttcccc
     6781 ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa atccaaaact
     6841 ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg ttcttggcta
     6901 tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag aaatccctca
     6961 ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat gtccaatcct
     7021 gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa
     7081 aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa ttgctgtggt
     7141 aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa tcttttttac
     7201 gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac tgttccttta
     7261 agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg ttcatcattt
     7321 caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta agccatacca
     7381 attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc
     7441 gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga atactttcat
     7501 ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg gaactgcatt
     7561 cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct tttaacccgc
     7621 tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac tctgctaact
     7681 ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt ttgaacagca
     7741 aatattcttt actataattt gttactctcc ccatcattct tacctccgtc tcttttcatc
     7801 tacccataaa taaataacat aaataattct aaaaatatta ttgataatta tcaaaacata
     7861 tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata tcttgacaaa
     7921 ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa taacgttata
     7981 taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt ccactcacag
     8041 tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct aattgaagca
     8101 agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg ttctttacaa
     8161 gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat ccgaaaaata
     8221 aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa tcataaagga
     8281 gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc taagttaaaa
     8341 ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat caattatcca
     8401 caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc ctcctatctc
     8461 gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg gatcctatct
     8521 gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta tctttacttt
     8581 tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga attaaatgca
     8641 ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc
     8701 tacttgctgt taactttatt catattacga aacaaacaac aggcatattt tccaaaaact
     8761 tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta ttcagaatac
     8821 tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag agcctacttt
     8881 aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa acaggagatt
     8941 ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca attgatgact
     9001 tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat ttatttttat
     9061 tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag tagtttttct
     9121 acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca ggaaaagaaa
     9181 ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca aatcagtcaa
     9241 taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca gttactcatc
     9301 catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca tttgcagcag
     9361 ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga tttgatcatt
     9421 acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga tacaacgcac
     9481 aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat gaaaaaaggt
     9541 tatgagaaca aaacaaagag gatgtgacaa aattcttgtc acatcctctt att
//
LOCUS       DAZKOW010000108         9570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000108 DAZKOW010000000
VERSION     DAZKOW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.108"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..818
                     /locus_tag="ACG912_002450"
     CDS             <1..818
                     /locus_tag="ACG912_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0513734.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ECYFSRFSLATREAVQWIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTARLKGTSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIVD
                     RLLSACPNLRQGYQLYQDILYAVKKRDQSLFEDCLNREVSGLPETYTTTLRTFKKFLP
                     QIQNALHYSYSNGPLECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGKTFLTK"
     gene            938..1138
                     /locus_tag="ACG912_002451"
     CDS             938..1138
                     /locus_tag="ACG912_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0513735.1"
                     /translation="MIEEEQKNIWDRYYKADPSSKNTKIGESGLGLSIVKELVRLHHG
                     SIEVTSRKNEGTTFLIQFPKEK"
     gene            1214..1411
                     /locus_tag="ACG912_002452"
     CDS             1214..1411
                     /locus_tag="ACG912_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513736.1"
                     /translation="MVECLKNKKMEANFVKAVISGSIVFQEEYRDMEKDFSKKGIELI
                     DYPRSCPILDKEYPEILTTFF"
     gene            complement(1708..3255)
                     /locus_tag="ACG912_002453"
     CDS             complement(1708..3255)
                     /locus_tag="ACG912_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFQ0513737.1"
                     /translation="MNITFLETSDMHGYVSPTDYSGRTDLALGTAKVSSKLKELRAKA
                     DGPVITIENGDFIQGSPLSYYLAKEYGSAKELTDLINQMSYDVQVLGNHEFNYGVDYL
                     EEAIKNYQAPILGANMVNKNDQPYFGNAYKIIEKVGVKIAILGLTTQYIPHWEKPETL
                     AGITFKSIVETAKEYVPLLRKQADLVIVSYHGGFEKDLLTGEDTEALTGENEGYELLQ
                     QVEGIDALFTGHQHRKIAETVHGVPVVQPGYRGEFIGEIRLTVEKKEGQVVVTDHQVA
                     LHSVEKSEPDPEILAAIVPVEEKLEKWLDQPLGKVNGNMRINDPMQARLEEHPYVEFI
                     NRVQLFASGSEISGTALFNNEGKGFGETITMRDVITNYIYPNTLAVIQINGADLRAAL
                     EQTANYLAVENNQIVFNPAFIQPKPQYYNYDMYEGIQYTIDMKQPVGQRITELRFKGH
                     PVLPQQMLEVVTNQYRAIGGGNYAMFGAEKIVREIQIDMTELIADYLNIHPRIEAKVN
                     HNFQVIL"
     gene            complement(3339..4142)
                     /locus_tag="ACG912_002454"
     CDS             complement(3339..4142)
                     /locus_tag="ACG912_002454"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFQ0513738.1"
                     /translation="MNILHISDIHFRREYEACEEGYKGMLATMQNPLIPLEQCIHHLL
                     QQTPLDLVIISGDLTEDGEIDDYRYLKTWLENALGETTILVTLGNHDIKEHFWVGWCD
                     EAASSAPYNQIVKYPEFTVISFDNSSYGYADGIVDDQQFQWLKKALEQEKDQPIFLVT
                     HHHLLSHQSSIPKWPGTERFLKLIAPYDIRCILNGHTHHTFTDEINGIPYFTVSSMSF
                     VGEDEGDGFVRFEERHGYNLYHLDQGRIVRQTTENFIPGNILKTLQMKD"
     gene            complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="ACG912_002455"
     CDS             complement(4145..4927)
                     /gene="phnE"
                     /locus_tag="ACG912_002455"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0513739.1"
                     /translation="MMLREMNRRTLLSLVALVILVILSAATLDYSGLSTFSPSMGMDV
                     LRGLGQPDWQFLYDGSGEDLISLLLLTIGIACLGTMIATVLAIPITLISAVNLWQAHP
                     WVTKIGKLICNVLRAFPELVFAIIFVKVVGPGPFAGVMAIGVHQIGMLGKLFTEEMEA
                     MDERLVEEMHAVGANFWQTIFFVRIPYLMPIYCSLALNHFEIAVRSAATLGLVGAGGI
                     GAPLIFAIQTRSWSKVSIILIGVVVTVFILDQITGIIRKRLR"
     gene            complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="ACG912_002456"
     CDS             complement(4924..5703)
                     /gene="phnE"
                     /locus_tag="ACG912_002456"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFQ0513740.1"
                     /translation="MSLKETVHFKWYKHLLILLFLIVCFQGSAWITSADFSQVFQNSN
                     QMTVFLSRFIHPDFSYLPKLVEPMVKTLQMSLLGTVIGLILAIPVSFLATTVVTENRL
                     LSTIFRFLLGLVRTIPTLLLAALFVAVFGIGEATGVLTIAVFTFGMVSQLMFQAIETI
                     DFGPIEAAAAVGANKMQIAIWSIAPQVLSQFASYAFYAFEVNVRASTVLGYVGAGGIG
                     VILNSSLALLNYERVSIIILTILVTVAIVDKLSERVRRSLL"
     gene            complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="ACG912_002457"
     CDS             complement(5675..6466)
                     /gene="phnC"
                     /locus_tag="ACG912_002457"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513741.1"
                     /translation="MIIFDNVSKVYPNGVVGLKDIDLTIEDGEFVSIIGLSGAGKSTL
                     LRSINRLAETTKGDIRINGTSITKANKKELRQIRRQIGLISQSFNLVKRSTVQKNVLS
                     GRLGYYSTWKSILGLFTKEDYQQTKEALEKVGLSDKLQTRSDELSGGQQQRVSIARAL
                     VQQAPIILADEPVASLDPITTQKVMMDLKNINQTMDKTVIVNLHSVPLAREFSTRVLA
                     LKAAQVVFDGRPDDLTDERLEQIYGNAIFEEKAGEIDELERNRSL"
     gene            complement(6527..7456)
                     /locus_tag="ACG912_002458"
     CDS             complement(6527..7456)
                     /locus_tag="ACG912_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFQ0513742.1"
                     /translation="MQFKQLAKGFIGVMLLTTLAACGSGNTSSSSEKESKEKLVVQFV
                     PTNNDGSMEAKAKPFAKYLSKKLDREVEVTLATDYSTIVEAMSSGQVDVGIMPPAAYV
                     QAKDAGAADAILTSQLGDYDQETGLPLKDKLTNTFKGEVLVRADSGLNKLTDLKGKNI
                     ATLSPNSASGYIYPVAELKDAGIDPTADTKMTTVNDIPSEITAVLNKQMDAAFVFEGA
                     RNVFASNFPDNDLFKDLKVLYLTKGDIPNDAIAVQPDMSKDLKAKIKETFLNMKDDKD
                     GKEAMALWGHQGYEEAADSAYDTIRDYTKKAAE"
     gene            7651..8334
                     /locus_tag="ACG912_002459"
     CDS             7651..8334
                     /locus_tag="ACG912_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HFQ0513743.1"
                     /translation="MKKIHDTKQLTAYVEKHNLQDYMDSDILAMASLLFFEKEEHLIQ
                     TGKTSDYLYFLVDGTVMVYSYSSDTQNICIDYAKPATPLGEASSLWELSPKSSVKAVT
                     KCICISIPLNQYRTQLQKDVRFLQNICQLLSYRLNSGINLANSLTEPVETRLAKFILT
                     HHENERFSFRLTTCASILNVSYRHLLRTITNFREENIIEKKKNYYLIHDIEALEDLAE
                     NISSPVKAE"
     gene            8495..9538
                     /locus_tag="ACG912_002460"
     CDS             8495..9538
                     /locus_tag="ACG912_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFQ0513744.1"
                     /translation="MKKILVSLLFLVSLVTLVGCSQSQGEKQSEKEKSKIALVLGFGG
                     VDDRSFNQAGWEGLEAWGKEKKLTAKKDFTYIETNNPEDNSTNIDQAIRNGFTTIISL
                     SYLQAEELENAAKDNPKLKFALVDGEITGKKNISSATFKDQESAYLAGLAAAYTTKSD
                     QVGFIGGMEGPVIQRFQSGFEAGVKAGATALNKEITVNVQYAASYDSLDKGKAIAASM
                     YKEGVDILYQAAGSTGAGVFQEAKARNENDYSRKVWVIGVDRDQADEGDYKMENGETG
                     NFTLTSTKKNVGAEVKEFAETSHNDKFPGGKHLTFGLKEEGVDLTKGQLSEEAYQAVS
                     DAKEKIISGDFRL"
BASE COUNT         2931 a         1956 c         1900 g         2783 t
ORIGIN      
        1 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
       61 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
      121 gcttgcctta tttagaatgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
      181 aatggattgt tattgatatg tatgcgcctt atgtttcatt ggtgaaaaag cttttcccga
      241 atgctcagtt gatcatcgac cgtttccaca tcgtccaaca tattggcaga accttcttga
      301 atcatcgagt gaaagaaacc acggcacggc ttaaaggaac ttcccactct caacgagggt
      361 tagggaaaaa attgaaacgc tattggaaac tcctacaaaa agaagaagca aagttaaact
      421 acgaaaaaag ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgtcg
      481 atcgactgtt atctgcttgt cccaacctgc gccaaggcta tcagttatac caagacattc
      541 tttatgcggt aaagaaaaga gatcagtcat tatttgagga ctgtttaaac agagaagtca
      601 gcggtcttcc tgaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
      661 tccaaaatgc gcttcactac agctattcaa atggaccatt ggaatgttta aacaaccaca
      721 tcaaagtatt aaaacgcaat gcgtacggct ttcgcagctt ttacaatttc aaactacgga
      781 tcatgattcg tcatggcaaa acatttttaa ccaaataaac agaacagaaa gagagtttct
      841 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacatcat ttgacaaaga
      901 gcctgtattt attactgtcg aggacaatgg catcggcatg attgaagagg aacaaaaaaa
      961 catttgggat cgctattata aagccgatcc gtcaagtaaa aatacaaaga taggcgagtc
     1021 aggtcttgga ttatcaatcg tgaaggaact agtgcgcctg caccacggtt caattgaagt
     1081 gaccagccga aaaaatgaag gaacgacctt cctgatacaa tttccaaaag aaaaataaaa
     1141 ttaccagtaa aaaaccgtgg agagatagct gctattgatt tttgctatgt cttttctttg
     1201 agaggtatcc ggtatggtgg agtgtctgaa aaataaaaaa atggaggcca attttgtgaa
     1261 agctgttatt tctggaagta ttgttttcca agaagaatat cgagatatgg aaaaagattt
     1321 cagtaaaaaa ggcattgaat taattgatta tcctcgctca tgtccgatcc tagacaaaga
     1381 gtatccagaa atcttgacga ctttttttta aatatcgcag cagcggattt attttttgta
     1441 tttaatcaag atcggaaagg ggtattaggc tatattggtg ccgcaacttt ttcagaaatg
     1501 aagtattgtc tgatgcaaaa tcttattcat ggaaaaagga tcaaaatagt cttatttaag
     1561 gaaccatcag aggaaaattt tttgttttga tgaggtagat cgctggctaa agaatggctg
     1621 gatggaaatc agacagccta acaatttggc ataacaaagg tgtgggcaaa aaactgctca
     1681 cacctttttg aattttttat ttatatacta caaaatcact tgaaaattat gattcacttt
     1741 tgcttcgatg cgtgggtgaa tattcaaata gtcggcaatt aattcggtca tgtcgatttg
     1801 gatttctcgg acaattttct ccgcaccaaa catcgcataa tttcctccgc cgattgcccg
     1861 atattgattt gtcaccactt ccagcatttg ttgcggaaga acggggtgac ctttaaaacg
     1921 aagttcggta atccgttgtc caacaggctg tttcatatca atagtgtact gaatcccttc
     1981 atacatgtca taattgtagt actggggctt aggctgaata aaggctggat tgaatacgat
     2041 ttggttattt tctacagcta aataatttgc ggtctgctcc aacgccgctc tcaaatctgc
     2101 accatttatt tgaatcactg ccagagtatt cggataaata tagttggtga tgacgtcccg
     2161 catcgtgatc gtctcaccaa agccttttcc ttcattattg aataacgcag ttccagaaat
     2221 ctctgagccg ctggcaaata attgtacacg attaataaac tctacatagg gatgttcctc
     2281 aagacgagcc tgcatcggat cattaatacg catatttccg ttcactttac ctaagggttg
     2341 atccaaccat ttctctagtt tttcttctac cggtacgatc gctgcaagaa tttctggatc
     2401 aggctctgat ttttcaactg agtgtaaagc aacctggtga tctgtaacaa ccacttgccc
     2461 ctcttttttc tcgacggtaa gacgaatttc accgatgaac tcgccacgat agcctggttg
     2521 aacaacggga actccatgaa ccgtttcagc aattttccgg tgctgatgac cggtgaaaag
     2581 ggcgtcaatg ccttcaacct gctgcaatag ctcgtagcct tcattctctc cagttagcgc
     2641 ttctgtgtcc tcaccggtta acaagtcttt ttcaaatccg ccgtggtaac taacgattac
     2701 caagtctgct tgtttcctta atagaggaac atattctttt gctgtttcga cgatactctt
     2761 aaacgtaatt cctgctaagg tttctggctt ttcccagtga ggaatgtatt gtgtcgttaa
     2821 tcccaagata gcaattttta cacccacctt ttcgataatc ttataggcgt ttccaaagta
     2881 tggttggtca tttttgttca ccatattggc tcctaatata ggcgcttgat aattcttaat
     2941 cgcttcctcg agataatcca caccatagtt aaactcatga tttcccaaca cttgaacatc
     3001 atagctcatt tggttaatca agtctgtaag ctctttggct gaaccatatt ctttagcaag
     3061 ataataactc aaaggagacc cttggatgaa atcaccattt tcgatcgtaa ttaccggtcc
     3121 atctgctttt gcccgtaatt ccttcagttt cgacgatact ttagccgttc ctaaggccag
     3181 atcggttctg ccggaatagt ctgttggtga aacatagcca tgcatatcac ttgtctctaa
     3241 aaacgtaatg ttcattctgc gtgctccttt agctgactca aaatagaaaa accgaagcgt
     3301 attcagctgt tcggtctctt tttttgattt ataatgcctt aatccttcat ttgaagtgtt
     3361 tttaaaatgt tgcctggaat aaagttttct gttgtctggc gaacaatcct tccttgatcg
     3421 agatgataaa ggttataccc atgacgttct tcaaagcgta cgaagccatc gccttcatct
     3481 tcaccaacaa aggacatact cgatacagta aaatagggga tcccattaat ttcatcagta
     3541 aatgtgtgat gggtatgacc gtttaggata cagcgaatat cgtaaggtgc gattagcttc
     3601 aagaaacgtt cggtaccagg ccattttggt atactgcttt ggtgagacag tagatgatga
     3661 tgggtgacca agaaaatcgg ttggtctttt tcttgctcta aggctttttt cagccattga
     3721 aattgctgat cgtccactat accatctgcg tatccatagc ttgaattatc aaaggaaatc
     3781 actgtaaact caggatattt gactatctgg ttgtacgggg cagaagaggc ggcttcatcg
     3841 caccagccta cccaaaaatg ttctttgata tcgtgattgc ctagagtaac aagaatcgtc
     3901 gtttctccta gcgcattttc cagccaagtc ttcaagtatc gataatcgtc tatttcgcca
     3961 tcttcggtta aatctccgct gataatcaca aggtccaagg gtgtttgctg caggagatga
     4021 tgaatgcatt gctccagagg gataagggga ttctgcatcg tagccagcat tcccttatag
     4081 ccttcttcac aagcctcata ctctctgcga aagtgtatat ctgatatatg caatatattc
     4141 attcttatct caatctcttt cttataattc cggttatttg atctaaaata aacacggtta
     4201 cgactacgcc aatcaaaata atactcactt tgctccaaga ccgagtttga atcgcgaata
     4261 tcaatggagc accgattccg ccagcgccaa ctaagcctaa ggtagccgca ctacgaaccg
     4321 ctatttcaaa atgatttaat gctaaagaac aataaatcgg catcaaatag gggatgcgaa
     4381 caaagaaaat cgtttgccaa aaatttgctc ctaccgcatg catttcttca acaagtcgct
     4441 catccatcgc ttccatttct tctgtaaaaa gtttgccaag cattccgatc tgatggacgc
     4501 caatcgccat aacaccagca aaggggccag gtccgacgac tttcacaaaa ataattgcaa
     4561 aaactagttc aggaaaagct cgtaaaacat tacagataag cttgccaatt tttgttaccc
     4621 atggatgtgc ttgccagagg ttaaccgcac tgatcaaggt aatgggaatc gctaataccg
     4681 tagcgatcat tgtccctaaa caggcgattc caatggttaa taatagcaaa ctaatcaaat
     4741 cctcgccgct gccatcatat agaaactgcc aatcaggctg acctaaccct ctcaaaacgt
     4801 ccattcccat ggaaggagag aaggtgctta atccagagta atccagagta gcggccgaca
     4861 aaataacgag aatcactaaa gcaactaatg ataaaagggt tcttctgttc atttcacgaa
     4921 gcatcataac agggacctcc tgacacgttc acttaattta tcaacgatcg cgacagttac
     4981 taggatcgtt agaataatga ttgaaactcg ttcataattc aaaagcgcaa gtgaggaatt
     5041 tagaataacg ccaataccac cagctccgac atatccaagg acagtagaag cacgaacatt
     5101 cacctcaaac gcgtaaaaag catagctcgc aaactgactc aaaacttgtg gagcgatcga
     5161 ccaaatagcg atttgcattt tatttgctcc aacagccgca gcggcttcaa tcgggccaaa
     5221 gtcaatggtt tcaattgctt gaaacattaa ttgtgacacc ataccaaaag taaagacagc
     5281 gatagtcaac acgccagtcg cttcaccaat tccaaacaca gcaacaaaaa gcgcggctaa
     5341 aagcagggta gggatggtcc gtactaaacc taaaagaaaa cgaaagatgg tagaaagaag
     5401 acggttttct gtcactacag tggtcgctaa aaaactaaca gggatcgcta aaattaagcc
     5461 aatgaccgtt ccaagcaaag acatttgaag tgttttcacc ataggctcga ctaatttagg
     5521 taaatagcta aagtctggat gaatgaagcg tgataaaaag acagtcattt gattggagtt
     5581 ttgaaaaacc tgagaaaaat cagctgacgt gatccaggca cttccttgaa aacaaacaat
     5641 taagaacaga agaataagca ggtgtttata ccatttaaag tgaacggttt ctttcaagct
     5701 catcgatttc gcctgccttt tcttcaaaga ttgcattgcc ataaatttgc tctaagcgtt
     5761 catcggttaa gtcgtctgga cgaccatcaa agacaacctg agcagctttt aacgcgagca
     5821 cacgcgtaga aaactcacga gccagaggga ctgaatgcag attgacaatg actgttttat
     5881 ccatcgtttg attgatattt tttaggtcca tcataacctt ttgagtcgtg attggatcta
     5941 atgatgcaac gggttcatcc gctaaaataa tgggggcttg ctgcacgagt gctcgcgcaa
     6001 tggatactcg ctgttgttgc ccaccactga gctcatcact gcgtgtctgc aatttatcag
     6061 acaaaccaac tttttccagt gcttcctttg tctgctggta gtcttccttc gtaaaaagac
     6121 ctaaaatact tttccaagtg gagtaatagc ccaaacgtcc agatagcaca tttttctgaa
     6181 ccgtactacg ctttaccaga ttgaagcttt gcgagattag accaatctgg cggcgaatct
     6241 gtcgcagctc ttttttattc gcttttgtaa tagaggttcc attaatgcga atatctcctt
     6301 tggtggtttc tgccaagcga ttgatactgc gcagcagagt gctctttcca gcaccactca
     6361 accctatgat ggaaacaaat tctccatctt caattgttaa gtcaatatcc ttcaagccaa
     6421 caacgccatt gggatagacc ttcgaaacat tgtcaaaaat aatcattatc cttctccatt
     6481 tttttaagtg taagcaaaaa aaagcttgct gacacttact gatgttttat tctgctgctt
     6541 ttttcgtgta gtctctgatc gtgtcatagg cagaatcagc agcttcttca tagccctgat
     6601 gcccccacaa agccattgct tctttgccat ctttatcatc cttcatattt aagaaggtct
     6661 ctttaatttt cgcttttaaa tcttttgaca tgtcaggctg tacagcgatt gcgtcattcg
     6721 gaatatcgcc ttttgtcagg tacaggactt ttaaatcctt aaataaatca ttatctggga
     6781 aattcgaagc aaaaacattt cgcgcgcctt caaaaacaaa cgccgcatcc atttgcttgt
     6841 tcaacactgc ggtaatctca ctcggaatat cattcacagt ggtcatcttt gtatctgcag
     6901 taggatcaat cccagcatct ttcaattcag cgacaggata gatgtagccg ctagcagagt
     6961 taggacttaa ggttgcgata ttttttcctt ttaaatcagt taacttattc aatccgctat
     7021 cggcacgaac caatacttct cctttaaagg tgttcgtaag tttgtccttt aatggcaagc
     7081 cagtttcttg atcataatcg ccgagctgag aagtcaggat tgcatctgca gctccagcat
     7141 cctttgcttg aacataggct gcaggcggca taattccgac atcgacttga ccagaagaca
     7201 tcgcttcgac gatcgttgaa taatcagtag ctaacgtcac ttctacctca cgatctaact
     7261 ttttcgataa atacttagca aatggttttg cttttgcctc catactacca tcattgtttg
     7321 ttggaacgaa ttgaacaact aatttttctt tgctctcttt ttctgagctg ctacttgtat
     7381 tcccagaacc gcaggccgcc aaagtggtca ataacatgac tccgatgaac ccttttgcta
     7441 actgtttgaa ctgcattttt ctcctcctgt tggatataat ttctaatacg tctaaatcat
     7501 agcagggaat caaaatcaat gaagtgacgc acgtcctatt ttacacaact tttacataca
     7561 atttacaaac aattataaat aattattcgt attgagccga aaaacaatct aagctataat
     7621 aaagaaaaac ttcttagagg tgacgatttc atgaaaaaaa tccatgatac gaagcaatta
     7681 actgcttacg ttgagaagca caacctgcag gattatatgg attcagatat attggcaatg
     7741 gcttctttac ttttttttga aaaggaagag cacctcattc aaacgggaaa aacctcagat
     7801 tatttgtatt ttttagtgga tgggacggta atggtgtact cgtattcttc tgatacacaa
     7861 aatatttgca tcgattacgc aaagcccgct acaccactcg gcgaagcctc ttcattgtgg
     7921 gaattatcgc cgaaaagcag cgttaaagca gtgactaagt gcatctgtat cagcattccg
     7981 ctaaatcaat atcgaacaca gcttcaaaaa gatgttcgct ttttgcagaa tatttgccag
     8041 ttactaagtt atcgtttaaa ttccgggata aatttggcga actcactgac agaacctgtt
     8101 gaaactcgtt tagcaaagtt tattttgacc catcacgaaa acgaacgttt ctcatttcgg
     8161 ttaaccacct gcgcctcaat cttaaatgtc agttatcgac atcttttacg aacaatcacg
     8221 aattttcgtg aggagaatat tatcgaaaag aaaaagaatt attacttgat tcatgatatc
     8281 gaagctttag aggacttagc tgagaatata agtagtccag taaaagcaga ataacagcta
     8341 taaaaaaaca gcagtcagaa gtgattgctg gttttttgat agtggagaaa ttgggaaacg
     8401 gaaaatcttc gttgaagagt agtgttctat atattggtat gctatacttt taaaagttca
     8461 atgtgtcaag acacataaaa aggggagaaa aattttgaag aaaatacttg tttcattgtt
     8521 gtttttggtg tcattagtaa cgttagtcgg ctgtagtcag tctcaggggg agaagcaaag
     8581 tgaaaaagag aaaagtaaaa tcgctttggt tcttggattt ggcggggttg atgaccgctc
     8641 cttcaatcaa gctggctggg aaggattaga ggcttgggga aaagagaaaa agctgacagc
     8701 aaaaaaagat tttacgtata tagaaacgaa caatccagag gataacagta ccaatatcga
     8761 tcaggcaatc agaaacggct ttaccaccat tattagcttg agctacttgc aagcagagga
     8821 actggaaaat gctgctaaag ataatccaaa gttgaaattt gccttggtag atggtgagat
     8881 tactgggaaa aagaatatct catcagcaac ctttaaagat caagaaagtg cgtatttagc
     8941 aggactagct gccgcatata cgacaaaaag tgatcaagta ggctttattg gtggaatgga
     9001 agggcccgtg atccaacgtt tccaaagcgg atttgaagct ggcgttaagg caggggccac
     9061 agcactgaac aaggaaatta ctgttaacgt acaatatgct gcatcctatg attcgctaga
     9121 taaaggaaaa gctattgctg cttctatgta caaagaggga gtcgatattc tttatcaggc
     9181 ggcaggaagt actggagctg gggtgttcca agaagcaaaa gcccgcaatg aaaacgacta
     9241 tagcagaaaa gtatgggtga ttggtgtaga tcgcgatcaa gcggatgaag gggattataa
     9301 aatggagaac ggcgagacag ggaatttcac gttgacttct acaaagaaaa atgtcggtgc
     9361 agaagtcaaa gaatttgcag aaacttctca taacgataaa tttcctggcg gcaagcattt
     9421 gacctttggt ttgaaagaag aaggggtaga tctgacaaaa ggacaattat cagaggaagc
     9481 ctatcaggca gtttcagacg caaaagagaa aatcatcagt ggtgatttca gactgtagac
     9541 aaagtccatt ttatggatga tgtctacagt
//
LOCUS       DAZKOW010000109         9485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000109 DAZKOW010000000
VERSION     DAZKOW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.109"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            168..362
                     /locus_tag="ACG912_002461"
     CDS             168..362
                     /locus_tag="ACG912_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513745.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="ACG912_002462"
     CDS             631..930
                     /locus_tag="ACG912_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513746.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="ACG912_002463"
     CDS             complement(1027..1461)
                     /locus_tag="ACG912_002463"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0513747.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="ACG912_002464"
     CDS             complement(1693..2403)
                     /locus_tag="ACG912_002464"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFQ0513748.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="ACG912_002465"
     CDS             complement(2393..3253)
                     /locus_tag="ACG912_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFQ0513749.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="ACG912_002466"
     CDS             complement(3258..3896)
                     /locus_tag="ACG912_002466"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFQ0513750.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="ACG912_002467"
     CDS             complement(3911..4210)
                     /locus_tag="ACG912_002467"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0513751.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="ACG912_002468"
     CDS             complement(4253..5737)
                     /locus_tag="ACG912_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFQ0513752.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGVLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="ACG912_002469"
     CDS             complement(5757..6218)
                     /locus_tag="ACG912_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0513753.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG912_002470"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="ACG912_002470"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFQ0513754.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="ACG912_002471"
     CDS             7521..8291
                     /locus_tag="ACG912_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFQ0513755.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="ACG912_002472"
     CDS             complement(8564..9391)
                     /locus_tag="ACG912_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513756.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2959 a         1939 c         1485 g         3102 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagacccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgtccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatgcttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaa
//
LOCUS       DAZKOW010000110         9455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000110 DAZKOW010000000
VERSION     DAZKOW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.110"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..74
                     /locus_tag="ACG912_002473"
     tRNA            2..74
                     /locus_tag="ACG912_002473"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="ACG912_002474"
     tRNA            78..150
                     /locus_tag="ACG912_002474"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="ACG912_002475"
     tRNA            155..225
                     /locus_tag="ACG912_002475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="ACG912_002476"
     tRNA            238..311
                     /locus_tag="ACG912_002476"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="ACG912_002477"
     tRNA            323..394
                     /locus_tag="ACG912_002477"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /gene="metK"
                     /locus_tag="ACG912_002478"
     CDS             660..1853
                     /gene="metK"
                     /locus_tag="ACG912_002478"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFQ0513757.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="ACG912_002479"
     CDS             1983..3455
                     /locus_tag="ACG912_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFQ0513758.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="ACG912_002480"
     CDS             3538..4326
                     /locus_tag="ACG912_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFQ0513759.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="ACG912_002481"
     CDS             complement(4393..5061)
                     /locus_tag="ACG912_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFQ0513760.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="ACG912_002482"
     CDS             5176..6888
                     /locus_tag="ACG912_002482"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513761.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="ACG912_002483"
     CDS             6891..8663
                     /locus_tag="ACG912_002483"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513762.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8745..>9455)
                     /locus_tag="ACG912_002484"
     CDS             complement(8745..>9455)
                     /locus_tag="ACG912_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0513763.1"
                     /translation="TGMIFKFANRRYGLKYNPVNGVSLARKPKTNEEIKKEKDGYLSK
                     EEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELISLTESDYDGNKITVSGTYDYE
                     LKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAY
                     NQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTH
                     TTKNMQNQLVEKLGKIEI"
BASE COUNT         2839 a         1585 c         2046 g         2985 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc agaaaagggg caatttaaat ttctattttt
     8761 cctagttttt ctactagttg attttgcata tttttagttg tatgagtata aatctttaat
     8821 gttgtttttg aatcttcatg tcctacacga tccataatgg cttttaatgg gattcctaat
     8881 tctgtaagta gagaaatgtg ggaatgcctt aacatgtgag aagaaacttt tttatctagg
     8941 tttaattctt tggatactgc tcttaaagat tgattatatg cttgaataga tatcgggtta
     9001 ccatttttag agatgaaaat gtatttatct ttattattct taattaattt gttttcttca
     9061 attaattgat ctatgatttc tttagcatta cttgataatt caatggttct ataagaacct
     9121 gtatttttag ttgttgtttt aatttttaac tcgtaatcat aagtaccaga aaccgtaatt
     9181 ttattaccat cataatcaga ttcagtcaaa gatatcaatt caccataacg tagtccagtt
     9241 aaaaacaaaa atttagtaat taaagaataa cgtgactgtt gatgtgaact tttttgttta
     9301 ctagcaatca tttgaacttc ttcttttgat aaataaccat ctttttcttt ttttatttct
     9361 tcgtttgtct taggttttct tgctaaagaa accccattta cgggattata ttttaatcca
     9421 tatcgacggt tggcgaattt aaaaatcatt cctgt
//
LOCUS       DAZKOW010000111         9028 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000111 DAZKOW010000000
VERSION     DAZKOW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.111"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..1245
                     /locus_tag="ACG912_002485"
     CDS             73..1245
                     /locus_tag="ACG912_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HFQ0513764.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
     gene            1596..3086
                     /locus_tag="ACG912_002486"
     CDS             1596..3086
                     /locus_tag="ACG912_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0513765.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
     gene            3434..3604
                     /locus_tag="ACG912_002487"
     CDS             3434..3604
                     /locus_tag="ACG912_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513766.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            3618..4235
                     /locus_tag="ACG912_002488"
     CDS             3618..4235
                     /locus_tag="ACG912_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0513767.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            4235..5173
                     /locus_tag="ACG912_002489"
                     /pseudo
     CDS             4235..5173
                     /locus_tag="ACG912_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(5866..6513)
                     /locus_tag="ACG912_002490"
     CDS             complement(5866..6513)
                     /locus_tag="ACG912_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0513768.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(6643..7581)
                     /locus_tag="ACG912_002491"
     CDS             complement(6643..7581)
                     /locus_tag="ACG912_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0513769.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            8072..9028
                     /locus_tag="ACG912_002492"
                     /pseudo
     CDS             8072..9028
                     /locus_tag="ACG912_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         3212 a         1481 c         1582 g         2753 t
ORIGIN      
        1 taaaaggcca tgtaacagcc cttttacggt acaatgtttt taaccacaaa aacataccca
       61 ggaggacgtt acatgaccca agtacatttt acactgaaca acgaagaggt tcaaagtatt
      121 attgaacatt cggtaaaaga tgatgtttct aaaaatattt taaccactgt tttcaaccaa
      181 ttgatggaaa atcaacgaac agaatatatt caagccgatg actatgaacg ttcagaaagt
      241 cgtcagagtc aaagaaatgg ctattatgag cgagacttta cgactcgtgt gggtacactc
      301 gaattaaaag tgcctagaac acgtgatggt gaattttcac cgacggtgtt tgagcgttat
      361 cagcgaaatg aaaaggcact gctcgcttca atgcttgaga tgtatgtttc gggcgtttcg
      421 acacgtaaag tttcaaagat tgttgaagag ctatgtggaa aatctgtatc gaaatctttt
      481 gtttctagcc tgactgagca gttagacccg atggtcaacg aatggcagaa ccgttcactc
      541 tcaggtacga attatcctta tctgatgact gatgttctct acataaaagt ccgtgaggac
      601 catcgagtgc tttctaaaag ctgccatatt gcgatcggga taacagaagg tggcgaccgt
      661 gaaatcattg gcttcatgat tcaaaatgaa gaaagtgatg acacatggtc catcttcttt
      721 gaatacttaa aagaacgcgg cctaaagggg acagaactca tcatttctga tgcccataaa
      781 ggcctagtgt ctgcgattcg taagtcattt accaacgcaa gttggcagag atgccaggtc
      841 cattttttaa gaaacatctt cagttccatt ccaaaaaaga attcaaaacc gtttagagaa
      901 gcagtaaaag cgatctttaa gtttacggat attgaactcg ctcgaacagc taagaatgcc
      961 ttagtcggtg aatatatcga ccagcctaaa tatacaaaag cctgcgaaat attggataat
     1021 ggcttcgaag atgcctttca atacacggtt atcggaaata gtcataatcg gctaaaaagc
     1081 accaaccttc ttgaacgact gaaccaggaa gtccgcagaa gagaaaagat tattcggatt
     1141 tttcccaacc gaacgtctgc caatcgatta attggagctg tccttatgga ccttcatgac
     1201 gaatggctca gttctacaag aaaatatatt aagtttgatc aatgagagac cggtaaaaca
     1261 ttgtatagta ttttacacag gattatggac ttgacttgga gataagaaaa ttagcaaata
     1321 tctatacact agaaacgttt aagaaagagt tagaaaagag aaatatctac ttagaaacaa
     1381 aatcagataa gtatttttct tcggaggggg aagattatat atataagtta atagaaaata
     1441 acaaaataat ttattcgatt agtggaaaaa aattgactta taaaggaaaa aaatcttttt
     1501 caaaacatgc aatattgaaa cagttgaatg aaaaagcaaa ccaagttaat taaacaacct
     1561 attttatagg atttatagga aaggagaaca gctgaatgaa tatccctttt gttgtagaaa
     1621 ctgtgcttca tgacggcttg ttaaagtaca aatttaaaaa tagtaaaatt cgctcaatca
     1681 ctaccaagcc aggtaaaagc aaaggggcta tttttgcgta tcgctcaaaa tcaagcatga
     1741 ttggcggtcg tggtgttgtt ctgacttccg aggaagcgat tcaagaaaat caagatacat
     1801 ttacacattg gacacccaac gtttatcgtt atggaacgta tgcagacgaa aaccgttcat
     1861 acacgaaagg acattctgaa aacaatttaa gacaaatcaa taccttcttt attgattttg
     1921 atattcacac ggcaaaagaa actatttcag caagcgatat tttaacaacc gctattgatt
     1981 taggttttat gcctactatg attatcaaat ctgataaagg ttatcaagca tattttgttt
     2041 tagaaacgcc agtctatgtg acttcaaaat cagaatttaa atctgtcaaa gcagccaaaa
     2101 taatttcgca aaatatccga gaatattttg gaaagtcttt gccagttgat ctaacgtgta
     2161 atcattttgg tattgctcgc ataccaagaa cggacaatgt agaatttttt gatcctaatt
     2221 accgttattc tttcaaagaa tggcaagatt ggtctttcaa acaaacagat aataagggct
     2281 ttactcgttc aagtctaacg gttttaagcg gtacagaagg caaaaaacaa gtagatgaac
     2341 cctggtttaa tctcttattg cacgaaacga aattttcagg agaaaagggt ttaatagggc
     2401 gtaataacgt catgtttacc ctctctttag cctactttag ttcaggctat tcaatcgaaa
     2461 cgtgcgaata taatatgttt gagtttaata atcgattaga tcaaccctta gaagaaaaag
     2521 aagtaatcaa aattgttaga agtgcctatt cagaaaacta tcaaggggct aatagggaat
     2581 acattaccat tctttgcaaa gcttgggtat caagtgattt aaccagtaaa gatttatttg
     2641 tccgtcaagg gtggtttaaa ttcaagaaaa aaagaagcga acgtcaacgt gttcatttgt
     2701 cagaatggaa agaagattta atggcttata ttagcgaaaa aagcgatgta tacaagcctt
     2761 atttagtgac gaccaaaaaa gagattagag aagtgctagg cattcctgaa cggacattag
     2821 ataaattgct gaaggtactg aaggcgaatc aggaaatttt ctttaagatt aaaccaggaa
     2881 gaaatggtgg cattcaactt gctagtgtta aatcattgtt gctatcgatc attaaagtaa
     2941 aaaaagaaga aaaagaaagc tatataaagg cgctgacaaa ttcttttgac ttagagcata
     3001 cattcattca agagacttta aacaagctag cagaacgccc taaaacggac acacaactcg
     3061 atttgtttag ctatgataca ggctgaaaat aaaacccgca ctatgccatt acatttatat
     3121 ctatgatacg tgtttgtttt ttctttgctg tttagcgaat gattagcaga aatatacaga
     3181 gtaagatttt aattaattat tagggggaga aggagagagt agcccgaaaa cttttagttg
     3241 gcttggactg aacgaagtga gggaaaggct actaaaacgt cgaggggcag tgagagcgaa
     3301 gcgaacactt gattttttaa ttttctatct tttataggtc attagagtat acctatttgt
     3361 cctaatatga ttttagcagt ataattgact tggtgaatag gtcatttaag ttgggcataa
     3421 taggaggagt aaaatgaaaa aatttattta tcgagtttta gaaaatgacg aagtggtggc
     3481 tatttttaat gagcaacaat atgcgcaaga ttttatcgct tacgaaaaga caatttctga
     3541 taagcaattt gaaattgaaa aagtagatat tgctgattgg ttattgcaac cgagagaatt
     3601 ttagaggttg gttgaaaatg gctaaaattg gttatgcacg tgtcagtagc aaagaacaga
     3661 acttagatcg gcaattacaa gcgttacagg gcgtttctaa ggtcttttca gacaaattaa
     3721 gcggtcaatc ggtcgaacgc ccacaattac aagctatgct taactatatt cgtgaagggg
     3781 atattgttat tgttactgaa ttagatcgat taggacgaaa taataaagaa ttaacagaat
     3841 tgatgaatca aattcaaatt aagggggcaa ccctggaagt cttaaattta ccctcaatga
     3901 atggtattga agatgaaaat ttaaggcgtt tgattaatag ccttgtcatt gaattgtaca
     3961 agtatcaagc agaatcagaa cgaaaaaaaa ttaaggaacg tcaggcacaa ggaatcgaaa
     4021 ttgctaagaa aaaaggcaaa ttcaaaggtc gtcagcataa atttaaagaa aatgatccac
     4081 gtttaaagca tgctttcgat ttgtttttga atggtttatc cgataaagaa gttgaagaac
     4141 aaactggaat caatcgccga acgtttagaa ggtatcgagc aagatataac gtgacagtcg
     4201 atcaaagaaa aaacaatgaa aagagggata gttaatgagt acggttattt tagctgaaaa
     4261 accaagccag gcattagcct acgcaagtgc tttaaaacaa agcaccaaaa aagacggtta
     4321 ttttgagatc aaagacccac tatttacaga tgaaacgttt atcacctttg gttttgggca
     4381 tttagtggaa ttagcagaac caggtcatta tgacgaaaag tggcaaaatt ggaaacttga
     4441 atctttgccg atttttcctg atcgatacga ttttgaagtt gcaaaagata agggaaagca
     4501 gtttaaaatt gttgcagaac ttctcaaaaa ggcaaataca attattgttg caacagatag
     4561 cgacagagaa ggtgaaaata tcgcctggtc gattatccat aaagcaaatg ccttttcaaa
     4621 agataaaaca tttaaaagac tatggatcaa tagcttagaa aaagatgtaa tccgaagcgg
     4681 ttttcaaaat ttgcaacctg gaatgaatta ctatcccttt tatcaagaag cgcaaacacg
     4741 ccaaattgcc gattggttga tcggcatgaa cgcaagccct ttgtatacgt taaatttaca
     4801 acagaagggc gtacaaggta cattttcact aggacgtgtt caaacgccca ccttatacct
     4861 tatttttcag cgccaggaag ccatagagaa ttttaaaaaa gaaccttttt tcgaggtgga
     4921 agctagtata aaagtaaacc aagggtcgtt taagggcgtt ctaagcccca cacagcgttt
     4981 taaaacccaa gaggagcttt tagcttttgt ttcttctaaa caagctaaaa taggcaatca
     5041 agaggggata attgctgatg ttcaaaccaa agagaagaaa acgaatagtc cgagtttgtt
     5101 ttctttaagt agtttgcaat caaaagtcaa tcagctttat aaagcgacag cgagccaaac
     5161 tttaaaagct atttcttttt taataactta aaaataaact taatgtaaca gcaagcacag
     5221 tcaaggtata cacctttgac aaaaaatagc acattctcta tcgaaaattt ttgcttattt
     5281 tttaaattat tttgggaaat tttcccaatc cctttttcta actcaaaaaa tataatcact
     5341 caaaatttaa aagggcgcac ttatacatca ttttaaaaaa ttgatgtaac gtgctaagtt
     5401 caaaacaaag ggcgcactta tacacgattt tcaatcttgt atatttctaa cgaaaagcgt
     5461 gcgccaaaaa acccccttcg tcaattttga cagggggctt tttgatgtaa aaatttctat
     5521 cgaaatttaa aaattcgctt cactcatgtt ataaagactt aaaataaaat aactctttaa
     5581 aatcttttgc tagttgttct tcaatatttt ttattcggtg catcttccaa gtaaagtata
     5641 acacactaga cttatttact acgtttcata agtcattaat gcgtgtgctc tgcgaggcta
     5701 gtttttgtgc aagcacaaaa aatggactga ataaatcagt ccataagttc aaaaccaaat
     5761 tcaaaatcaa aaccacaagc aaccaaaaaa tgtggttgtt atacgttcat aaattttatg
     5821 atcacttacg tgtataaaat taaattcact ttcaaaatct aaaaactaaa tccaatcatc
     5881 taccctatga attatatctt gaaattcatt cataaatagt gaagcatggt aaccatcaca
     5941 tacagaatga tgaagttgca gagcaactgg tatataaatt ttattattct cactataaaa
     6001 tttacctatc gtaataatag gcaataaaaa gctgctattg ttaccaatat ttaaattaaa
     6061 tgaactaaaa tcaatccaag gaatcattga aatcggtatg gtgttttcag gtatcggttt
     6121 tttaggaaac atttcttctt tatctttata ttcaagcaag tcatttttat aattattata
     6181 aaaagaaatg aagtttttat cagattcagt ccaaatgtta gtaaattttt cagtttgctt
     6241 attaaaaact gtatacaaag gatttaactt atcccaataa cctaatttat tctcactatt
     6301 aattcctgtt ctaaacactt tatttttatt tacaacttcc ataattgcat aaattaaaga
     6361 gggataaatt tcatatcctt tcttttttat catatcttta aacaaagtaa tatcaatttc
     6421 tttagtaatg ctataagtag tttgctgatt aaaatagtgt tcaaaatatt cttttctatc
     6481 ccaattttct aattcaataa tattaaaagt catatataac ttcctcctaa attttaaatt
     6541 tttatattta ggaggaataa tcctctgatt ttttcatacg ttatgtcacc tcgtaaatat
     6601 taattatact gaattagcaa tttttatcaa ataaaactta ttttacttcc aaaacctaaa
     6661 ttcacgttgc caaaaatcaa tctgcttttg caattgtttt tcgttcgctt ttaaagtcga
     6721 tttcattaat tccgttaaat caattggaga tatttctcta atcaattttt taaatttagt
     6781 cttagtattc ttacttagct ttccccacat actttcttca tgcaacaaag tataaaccat
     6841 agcttgctca ttaatttttt ctaaagtagc ccacgcaggt ttcaagatgt gtaaatcatt
     6901 aaaacaatca ttccagtaat caaccatatc tctttttaat tcaacttcta cacgccataa
     6961 atgttcagac acaacttcaa catctgcgtt atctttacgt tcttgttttt tattataaat
     7021 tctaataaat ctatcactat cacggacacc aaaatatttt gtttctggct tgccattacg
     7081 accataaaaa acagttttct taactgcttt atcagtcatt gcatagtaat cgctcaaatc
     7141 atcttcaaaa tcaaaagcta agtctaatct tgtaaaaccg tcatcttcca tgtagtcgat
     7201 aatattttgt tttaaccaaa tcatttcttc atgtgtgagt ttattgggat taaattcaac
     7261 acgcatatta cgtctatccc aagtatctgc ttttactttg tcatattcga tataaacttt
     7321 ttcttgaagt gccttagctt taaactttgt ttgaagtata tcccaaagtc gtatttgtgg
     7381 ctctacactc ataaagtcag atagcttttt agcattagtt ttgttcaaat ttccaacgat
     7441 tgtcatggca tcaaaactta atgcgggttg agattttccc aaagtttgac cacttaaccg
     7501 gctattactt aaccggctat tagagacggc cggttttttt agtgagattt ctgcagctaa
     7561 tttttcatca attttcttca ttttttatcg ctcctaaaat aatttttgga acgaaaaaag
     7621 gacgagtgga atcgcaaaat aaatttcgcc tgtatccatt cgtccatttt gttttgtatt
     7681 caaaatcaaa aattgatata ctaatcatcg aatgaataac aagtgtggtt tgttattccg
     7741 ggcgagaacg ttggtagcgt tgctcgtctt tttttatgtc tttttttcgt taaaactatt
     7801 ttaaaactaa atatatctta aagcaaataa attcaagggg ctaaaagcgc cccttaaaaa
     7861 ccccttgcct ctgaaaagtt ttgtgtttcc tttccgtcgg tttcaaaagc taatttactc
     7921 aaaaaaatag atcaagggta aatgcactca ctgcgttcgc ccttgagcta ttttatttcg
     7981 caaattttaa cgcatttgcc gacgaaaaga taaaacacat aaaaatatac gcagatatat
     8041 ttaaaacgtc ttagaacgca aaattttacg tttaaaagct atgcaaggac tgtatgaagc
     8101 aaaattattg agttatccaa gaacagatac accatttatt acagagaacg aatttgctta
     8161 tttaaaagcg aattttggca aatatagcgg ttttttagga cttgatcttg aaatggttca
     8221 aacagagcct agaaagcgtt atgtggacgg tagtaaggta caggaacacc acgccattat
     8281 cccaacaaaa caagtaccta ccgaatctgc attagcgaaa atggacgatt tacaacgaaa
     8341 aatttatgct ttagtcgtta aaacgaccgt tgccatgttt ttacctgatt acttgtatga
     8401 agaaaccaag atacaaacga aagtagctga tttgctgttt caatcgatcg gaaagacacc
     8461 aaagcaagaa gggtggaaaa ttcttttcaa acaacaaacc aaagaagaaa aagaggacgt
     8521 tcaaacgtta ccactcgtta tcattggcga acgtgccgaa gttggtgtta agagtgttga
     8581 aaaagaaacg caaccgccaa aagcttttac agagggtaca ttattaactg ctatgaaaac
     8641 ggcgaataaa acggttgatg atgaagaagc aatcaagatt ttacaagaag ttgaggggat
     8701 tggaacagaa gcgacaagag caagtattat tgaagcgtta aaacaaaaag aatatatcca
     8761 agtgattaag aataagcttg ttgtaactga aaaaggaaaa ttattgtgcc aggcagttga
     8821 aagtcagcac cttttaacga gtgctgaaat gacggctaaa tgggaaacgt atttaaaaaa
     8881 aatcggtaaa agagaaggca atcaagagaa ctttattacg aatatcaaaa aattcattgt
     8941 tcatttactg gaagctgtac ctaacgatat agaaaaacta aatttttctg attaccagga
     9001 acagaaagaa aaagaagcag aaaaaagt
//
LOCUS       DAZKOW010000112         8851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000112 DAZKOW010000000
VERSION     DAZKOW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.112"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            14..1141
                     /locus_tag="ACG912_002493"
     CDS             14..1141
                     /locus_tag="ACG912_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0513770.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1359..2501)
                     /locus_tag="ACG912_002494"
     CDS             complement(1359..2501)
                     /locus_tag="ACG912_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0513771.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2506..2691)
                     /locus_tag="ACG912_002495"
     CDS             complement(2506..2691)
                     /locus_tag="ACG912_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFQ0513772.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3045..3290)
                     /locus_tag="ACG912_002496"
     CDS             complement(3045..3290)
                     /locus_tag="ACG912_002496"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513773.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3284..3685)
                     /locus_tag="ACG912_002497"
     CDS             complement(3284..3685)
                     /locus_tag="ACG912_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0513774.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACG912_002498"
     CDS             complement(3872..5680)
                     /gene="uidA"
                     /locus_tag="ACG912_002498"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFQ0513775.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5703..7469)
                     /locus_tag="ACG912_002499"
     CDS             complement(5703..7469)
                     /locus_tag="ACG912_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HFQ0513776.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7482..8276)
                     /locus_tag="ACG912_002500"
     CDS             complement(7482..8276)
                     /locus_tag="ACG912_002500"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFQ0513777.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(8286..>8851)
                     /locus_tag="ACG912_002501"
     CDS             complement(8286..>8851)
                     /locus_tag="ACG912_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0513778.1"
                     /translation="AQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSA
                     IVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTV
                     YAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIV
                     GLILFFLYPLSKDKVLENCELLKQKHK"
BASE COUNT         2868 a         1696 c         1181 g         3106 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg
     1201 tttatttttt acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca
     1261 ttttcgatac cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata
     1321 ggctatgaca gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt
     1381 tcatttgcga gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt
     1441 ttaatagagg catgacctag acgcttagat attgcataga tatttttatt atgttcaatt
     1501 aaaaaggcaa catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt
     1561 tcagctagtt tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa
     1621 gaaaaaacca attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa
     1681 tcttcaacgg tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca
     1741 gcaaattcat aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca
     1801 tttattaagt caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta
     1861 acaaaaataa aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca
     1921 ctattatata ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt
     1981 tttattttac ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt
     2041 ttaaaaacta ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc
     2101 tcaatgccct tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga
     2161 attggtcgaa tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata
     2221 tgatctgttt tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac
     2281 tctcgataaa aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa
     2341 ctttgtttta actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg
     2401 gtcacttgaa ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg
     2461 cctctttttc tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga
     2521 aatatggaga acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata
     2581 aaaaaagtat cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata
     2641 gtactgagaa aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc
     2701 ttccttactt tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt
     2761 cacctctccc caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca
     2821 tcgcttttta ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa
     2881 gaaatcatgg cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt
     2941 taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta
     3001 gaagattctt ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat
     3061 tttaagatcg cttctattaa tttaatttct agctgactta tcatatattc atctacatag
     3121 gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa
     3181 tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata
     3241 atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc
     3301 ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat
     3361 tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct
     3421 ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata
     3481 tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc
     3541 cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa
     3601 tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag
     3661 atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata
     3721 caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat
     3781 cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag gctaggctaa
     3841 tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa ttgggtattg
     3901 atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct ctagtaaaga
     3961 ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa tctgcaaaat
     4021 tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga tttgcttcgt
     4081 aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga aatccagcta
     4141 ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt tccatccatc
     4201 catctagctc ttttttcaac agatattttg catgtgccag atcccctccc gaaacatacc
     4261 aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta caaacatctg
     4321 gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca agttctttag
     4381 ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa tctggttcat
     4441 ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc aattccttga
     4501 tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt ttttccacat
     4561 tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt acttcatcaa
     4621 taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa ggataatgtg
     4681 atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta atatttacag
     4741 cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat cctttaaaat
     4801 aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg cgaataccaa
     4861 attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt ttcatactat
     4921 ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc attcctttct
     4981 ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt aagatttttc
     5041 cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt attcttgtat
     5101 taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca ggtctatgaa
     5161 tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata tccccatttt
     5221 cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata atattgttta
     5281 cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca gactcaaatg
     5341 gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact tttgcttcat
     5401 gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta ggaagagtca
     5461 cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt tcaaataaat
     5521 cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt ggttctaaag
     5581 caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga ccgcttaagt
     5641 caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact cctatctaaa
     5701 tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac tcccttaaat
     5761 tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt tccatcataa
     5821 gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca ctgtacagat
     5881 acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc tttacttgct
     5941 ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat gatcaagtca
     6001 tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc tatattttta
     6061 attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta tttttcaact
     6121 tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg tctatttcca
     6181 gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa aatcctttta
     6241 tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt aattgcttgg
     6301 tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc cagagcttct
     6361 tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt agcttttaac
     6421 cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc tttacgatag
     6481 ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa tagatcacat
     6541 ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt cattcctaac
     6601 atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct aagtaagtca
     6661 gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag atcaggatta
     6721 aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg taatccagct
     6781 tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt acctaaacta
     6841 ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg gaaatgttta
     6901 gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact aagttctaaa
     6961 actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct ccaatttcta
     7021 ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc ttcaatccca
     7081 gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat ttttacttct
     7141 acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt attagcagca
     7201 attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata tacctcatca
     7261 gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat attactcaag
     7321 taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt ttcttccaaa
     7381 gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa attatatggt
     7441 ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt agtaagattt
     7501 ttccaaaaag tatctatata tttttgttca atatctacat aatctcccga taaacattta
     7561 aataaaacat cagttcttaa gttttcccat gattcttttt ctttattgac caaaatagga
     7621 taaaagaaaa ctttattttt atttgcagct tctagatcac caggagaatc accaatcatt
     7681 aatatcttat ccaaatcaca accattttca attacacttc gaataatatc ttcttttttt
     7741 cctttatctt gacaacacag ttcatcaact aatgataata attcatgtct ttcccattct
     7801 tcttgaacag cttccttatt agctgagctt acaataatta tcttcccata ttttttcaga
     7861 gcttttatag tatctctagc acttaaaaag ggtttatcta atccttttgc ttgactaatc
     7921 tgttgattga cttcttccga ccattttaaa gcttttaata aatcctctga ctgagaatat
     7981 tctatttccc tcttcaaaga tggattagac aattcttttg ttagagatac ccagtgcttt
     8041 aatcgattaa tcgatgttac atcatttttt tctaatgttt taactaaacc taaaaatctg
     8101 tttattccac gagtctctga atataaatta atattattcc aatcctgtag gaatttttct
     8161 ctatgatgaa catcaaaaat ctctgcagca attggtccga aaaagagatt gtgtttataa
     8221 gtcatcgtat ccataacaca gccatctgaa tcaatacaaa atatccaatt attcatattt
     8281 ctctcctatt tatgtttttg ttttaaaagc tcgcaatttt ctaagacttt atctttagat
     8341 aatggataaa ggaaaaataa aattaatcct acaatcagaa aacaaattga tggaattaat
     8401 gttgaagcgt tatataactt ttctaaaaca tctcccgttt gtacatttgc taaagagtca
     8461 tagcctataa tagaaagagt ccaacctccg acgcctccag ctagagcttg acctattttt
     8521 ctagcaaaag aatataccgc atatacggtc ccatcttctc tttgatcaga ctttacttct
     8581 tgatcatcaa taacatcagt aatatttgcc caaataacag tattaaacac acccaaaccc
     8641 atatagccaa ccgaactaag aattaagaat acatacatat tactagcttt tatcaaaaat
     8701 aagatcaaat atactattgc tgaaaagaac attcctactg atgccagttc tttttttcca
     8761 tattttttac ttaaaaacaa agttaaaggc gaaacaacta ttaaaaccaa aataggattg
     8821 ataaaattca ttaaaactat tccctgtgca t
//
LOCUS       DAZKOW010000113         8652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000113 DAZKOW010000000
VERSION     DAZKOW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.113"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(250..2631)
                     /locus_tag="ACG912_002502"
     CDS             complement(250..2631)
                     /locus_tag="ACG912_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFQ0513779.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2621..3268)
                     /gene="recU"
                     /locus_tag="ACG912_002503"
     CDS             complement(2621..3268)
                     /gene="recU"
                     /locus_tag="ACG912_002503"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFQ0513780.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3332..3871
                     /locus_tag="ACG912_002504"
     CDS             3332..3871
                     /locus_tag="ACG912_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFQ0513781.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3961..4380
                     /gene="gpsB"
                     /locus_tag="ACG912_002505"
     CDS             3961..4380
                     /gene="gpsB"
                     /locus_tag="ACG912_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFQ0513782.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4402..4769
                     /gene="rnpB"
                     /locus_tag="ACG912_002506"
     ncRNA           4402..4769
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACG912_002506"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4978..6144
                     /locus_tag="ACG912_002507"
     CDS             4978..6144
                     /locus_tag="ACG912_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFQ0513783.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6190..7686
                     /locus_tag="ACG912_002508"
     CDS             6190..7686
                     /locus_tag="ACG912_002508"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFQ0513784.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7767..8450
                     /locus_tag="ACG912_002509"
     CDS             7767..8450
                     /locus_tag="ACG912_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFQ0513785.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            8459..>8652
                     /locus_tag="ACG912_002510"
     CDS             8459..>8652
                     /locus_tag="ACG912_002510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513786.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         2720 a         1571 c         1837 g         2524 t
ORIGIN      
        1 gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag taaatggata
       61 aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa atgttagatc
      121 acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg gtacatgatt
      181 tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt agacgtggtt
      241 tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat ggtcatttgc
      301 agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag atgaagacgg
      361 tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg gctcgcttgt
      421 ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac tagtagagct
      481 agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat agctggaaga
      541 tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat ctttgtagta
      601 cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat ttgtcacgtt
      661 agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg acgattctga
      721 agtgacaggc gtcatttttt tattgtaacc tgtccacacg gaaatcgaat aatttggcgt
      781 atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt ttgcatactc
      841 gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag cgatttgcgc
      901 attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca tataagctgt
      961 ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc cgtcttggaa
     1021 gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga aagccgcata
     1081 ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt cttccgtatt
     1141 actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat tttttaagaa
     1201 agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa cattacggga
     1261 taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc agtttcctac
     1321 aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc cagtagaata
     1381 tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag aaccaaaatc
     1441 acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga tgtgacgtcc
     1501 gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg caacttgcat
     1561 ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata gtttcttttg
     1621 ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat tcttatctgt
     1681 tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct tagtattgtc
     1741 atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag cttgatcata
     1801 ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat ctcgtctttt
     1861 cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag gcatcccagc
     1921 aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt acatctcaga
     1981 agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt agtaggtcaa
     2041 gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt cttgggcctt
     2101 gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga tcaattgctg
     2161 tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg cagaaccgat
     2221 gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta ccgacacgat
     2281 cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct tttctgcgcc
     2341 taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg tagcgtctaa
     2401 ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc ctacacctgc
     2461 taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa ataatccttt
     2521 tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca cactcttttt
     2581 aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca ttcttgcttt
     2641 ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa ggaatccgcg
     2701 gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa ggtagtgatt
     2761 tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta ttcaaataaa
     2821 aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt tgttcgaggc
     2881 atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa gatgttttgt
     2941 tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca ttatagtctg
     3001 tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc ttaggatagt
     3061 ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac ccgcgggaaa
     3121 gataatattt attactttca ttgattgctt cttcaaagcg catcccccga ttgccgaatt
     3181 cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc ttataataag
     3241 gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca cattatagca
     3301 aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa tctatgtgtc
     3361 tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa tccaagttgt
     3421 taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg aatgggtact
     3481 tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg aattgaaaga
     3541 agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg gtaataactg
     3601 gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt atgtcgaagc
     3661 tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc gttttatact
     3721 tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa aaacgaagta
     3781 tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc ggctgatcag
     3841 tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt atcaactttt
     3901 ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag aggtgtaaat
     3961 atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa aacaaaaatg
     4021 cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa agattatgaa
     4081 acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag tgcaaaagta
     4141 gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac agaggcacca
     4201 aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct tgaacgtgaa
     4261 gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca acctaatcca
     4321 aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg tcaattttaa
     4381 tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat caggctgagg
     4441 aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg aaaccataag
     4501 ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct atgcttaagt
     4561 atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg gaacgcggta
     4621 aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg aagaagaaca
     4681 ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt ctccctggaa
     4741 aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt aggtcgtgac
     4801 aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc aatacttcag
     4861 atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga atttgagtaa
     4921 aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag attgtgtatg
     4981 gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga agcactggtt
     5041 ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg tgcacgtttt
     5101 gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc agatcgagta
     5161 aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga aaacgtaaaa
     5221 gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc tggaaaatcg
     5281 atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa agcaatcgtt
     5341 gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga aactggcgca
     5401 cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct ggatacgaca
     5461 ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc attgaaagaa
     5521 aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc attttatgat
     5581 cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca taatatcgca
     5641 ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca agaaatcatg
     5701 gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt agatattact
     5761 ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga agaaatcgga
     5821 ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac ggataaagaa
     5881 tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga agaaactgtt
     5941 cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg gagtaaatat
     6001 atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac caaaaaaaga
     6061 aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac tcgtccgcct
     6121 agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg atgtttggag
     6181 gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa tcttctacaa
     6241 caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa agctagtgca
     6301 taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa aatcggtcca
     6361 aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc ggaagttgga
     6421 actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt accggaagat
     6481 cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca aaaagctaga
     6541 gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga attaacaaaa
     6601 cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt gctgaatcaa
     6661 tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct taaagaagga
     6721 attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac agacttttta
     6781 aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat cgaacaactt
     6841 ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat gactggaatt
     6901 aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa aatggctgtt
     6961 tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga tgataaatat
     7021 gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca atcgttgttt
     7081 aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc tttctttcaa
     7141 gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg ttcattgaac
     7201 catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc tctccatatc
     7261 atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt tgctgaacta
     7321 cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac aaatgatgtg
     7381 gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt tccctcttat
     7441 gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga aatagatgtg
     7501 gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc agagcatatc
     7561 catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac acatgaaccg
     7621 cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt atatcaggta
     7681 aaataaaata caatttacaa agatagctga aattcatcta atctatagat gttttcagct
     7741 atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct gattatagta
     7801 tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa agagttgtca
     7861 agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg acgcttgcag
     7921 cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac gaggcaaagc
     7981 aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc tcgatgattt
     8041 tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc gaactatctc
     8101 tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca atcggcattt
     8161 tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct gaactgtatc
     8221 cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc aatcattttt
     8281 atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat gaagaagcca
     8341 aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa tggtctattg
     8401 acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga tggaggttgt
     8461 gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt acttctggaa
     8521 cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag gaacaatttc
     8581 aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat tggcaggaga
     8641 acaccgttta tt
//
LOCUS       DAZKOW010000114         8494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000114 DAZKOW010000000
VERSION     DAZKOW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.114"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..149)
                     /locus_tag="ACG912_002511"
     CDS             complement(<1..149)
                     /locus_tag="ACG912_002511"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0513787.1"
                     /translation="MENHVLETGMQKITTHASITAKPFFEKRGYKVINEQTVELRGQL
                     FTNFL"
     gene            complement(593..1714)
                     /locus_tag="ACG912_002512"
     CDS             complement(593..1714)
                     /locus_tag="ACG912_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFQ0513788.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="ACG912_002513"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="ACG912_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFQ0513789.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="ACG912_002514"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="ACG912_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HFQ0513790.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="ACG912_002515"
     CDS             complement(4304..5215)
                     /locus_tag="ACG912_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFQ0513791.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="ACG912_002516"
     CDS             complement(5232..6239)
                     /locus_tag="ACG912_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFQ0513792.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="ACG912_002517"
     CDS             complement(6236..8365)
                     /locus_tag="ACG912_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFQ0513793.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8480)
                     /locus_tag="ACG912_002518"
     CDS             complement(8370..8480)
                     /locus_tag="ACG912_002518"
                     /inference="COORDINATES: protein motif:HMM:NF024254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513794.1"
                     /translation="MMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAZKOW010000115         8441 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000115 DAZKOW010000000
VERSION     DAZKOW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.115"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..3044)
                     /locus_tag="ACG912_002519"
     CDS             complement(<1..3044)
                     /locus_tag="ACG912_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0513795.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
     gene            complement(3661..4095)
                     /locus_tag="ACG912_002520"
     CDS             complement(3661..4095)
                     /locus_tag="ACG912_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513796.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(4112..5317)
                     /locus_tag="ACG912_002521"
     CDS             complement(4112..5317)
                     /locus_tag="ACG912_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFQ0513797.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            6011..6397
                     /locus_tag="ACG912_002522"
     CDS             6011..6397
                     /locus_tag="ACG912_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFQ0513798.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            6491..6682
                     /locus_tag="ACG912_002523"
     CDS             6491..6682
                     /locus_tag="ACG912_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513799.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            7196..7528
                     /locus_tag="ACG912_002524"
     CDS             7196..7528
                     /locus_tag="ACG912_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0513800.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7438..7632
                     /locus_tag="ACG912_002525"
     CDS             7438..7632
                     /locus_tag="ACG912_002525"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0513801.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            7671..7817
                     /locus_tag="ACG912_002526"
     CDS             7671..7817
                     /locus_tag="ACG912_002526"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513802.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            8255..>8441
                     /locus_tag="ACG912_002527"
     CDS             8255..>8441
                     /locus_tag="ACG912_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0513803.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHRHYQNEQTIIK"
BASE COUNT         2713 a         1531 c         1236 g         2961 t
ORIGIN      
        1 ccttctactg ttggctcata tttatcggcg tcagaacgat tgtctgtcac tgtaacatct
       61 acttcaactt catcagatga tccatctgga tatgtcacta caacagtccc ttttttattt
      121 ccagctgcgc taacatctac tttttctttc caagtatact tagtatcttt tggtaaatct
      181 tctttgttct taattccctc aaatgcgtct ggttccttat tcaattctgt agtcactttt
      241 tgaccttcag gattatattt gtcagcgtca gatttatcat ccagttttgt tgcacttgat
      301 acaaattctg tatcatctgc tgtaattggt tgagttgctt cctctggcca tttcgcatct
      361 gtataaccat cttttgcttt caacactgga agtttatctt ctggtaagct tacgccgtct
      421 ttcactgcaa agactgttgt tccactttca atacttgttc cttctcctgc ggtgaaggtt
      481 actttgtggt agcctgcagg aatgttttct cctggatttt caatgatatc atccagtttt
      541 gttgcacttg atacaaattc tgtatcatct gctgtaattg gttgagttgc ttcttctggc
      601 catttcgcat ctgtataacc atcttttgct ttcaacactg gaagtttatc ttctggcaag
      661 cttacgccgt ctttcactgc aaagactgtt gttccacttt caatacttgt tccttcccct
      721 gcggtgaagg ttactttgtg gtagcctgca ggaatgtttt ctcctggatt ttcataaata
      781 tctttactta cagttactgc taaatcggtc gtgcctacag aaccatcttg gtaaacaatt
      841 ttaataggaa ttgttccagt aatcggctcc gattctccta cacctttagc tggagtgaat
      901 gtaacaatac cagtgtttgc atcaacactt gcattaccga agtacttatt atagacatct
      961 gaaattgaaa aggcatcttt agctaaagga acatctggtt tatcaaatgt aggttttcca
     1021 ctttgaactg tcgtttctgg agttgttgtt ataggtacgt atgttgcatc attttccata
     1081 gcttgagaag cttttacagc aaactctaca tccacgtcga aagttcgatt tccgatctta
     1141 acagttacaa tacctttcgt actatcagta acgttagaag tatcaggctc tgttttccat
     1201 gaatactctg catcttcagg taagttttta ttattccgaa ttgattcttt agcatctggt
     1261 ttaaatccaa ctaccacggt ttgtttatca accgcttttg gtgattcctt aataacggtt
     1321 gaaccttctt ctggtttatc aaaacctgga gaaacgattt ggatttttaa ttcatctttc
     1381 gcgattaatt tacttgaatc tacacccgta aattcaaatt ctacagtatc tgaaattgga
     1441 gccccatctt tttcatttgg agcgatagtt ttttctgcta atacagtacc ttctttatca
     1501 acaagttggg ctttgtgacc tgttcccgct aactcgtgga tgaatacttt ccccttaact
     1561 gttgtgtcaa catcatatac ttcttctaat tcaggtttcc cgatatatgg attcgtgtct
     1621 ccgctctctt ctttatcgtc agggtttcca tcaccatcaa tatcttgcaa ataataataa
     1681 cctgttccat aagtgttctg gattaatttt ttattcgtat ccgacaacca agaatcaaag
     1741 atgaaatctt caccaaatgg tttattttct tcaacttgtg ctgttgcttt catatcttct
     1801 aagatttcat ctaatggctt agtcaattgt aaaacatatc gaataacgac tggacgtgga
     1861 cttgcattag caaaatcgat aagtttagtt aaatcattat tagttattga acctatgcca
     1921 taattttcat gaccttcccg tcgctcgatt gctaaatcag ctacttttgt tccggattca
     1981 aacgaaacat catatgttac tttgtcagcc tgaaccatgt gcatctgtat acccgctaca
     2041 tatggaacta atcgttcatc aattttgtaa ttagcatacc aaggcgtgcc ctttgcggaa
     2101 gatgaatatg agaaattata atttttcgtt aacttatgat ctaagattat agctccatta
     2161 gcacctgtgg atcctgaacc atcttttgtt tgagtattag gtttctcata ttttgcactt
     2221 actgatccat gtttaaactg gtcaggatta ttttcagata catctaagct attatagaaa
     2281 tcatctgaat ctgttaaaaa gtagcccgta ctatcaatac ccggtacaat tttatcatcg
     2341 tcttgatgac ttaccaagta gattcgatac attaaacgat cggtcttgag attgtctatg
     2401 cctatctctt taaaaatttc atctaggctc ttttcaaacg taattacccc attaggagcg
     2461 gtttgcgtat cggttgtttc tgccccagca aataatccat cacttgcacg aatataattg
     2521 accttccaaa tatttgtagg tctacccagc gaatcattaa ttcttacaaa ggtccgtcta
     2581 gaagatccaa ttcctttctt tggatcgacc tgaatttttt ctacatattt agctaatcgt
     2641 tcatctaatt gaagacggat ttcccagttt ttagttgatg ctgcaatatt atgttttccg
     2701 taaacttcaa atccaattgt attgcctttt agtgtgtctc cagttagttc ttgaggacta
     2761 aattgtaaat ccgaaaagtt ataatttttc gtataaccag gattctcctg aaaagtattc
     2821 cccgaagcta ctttgtcacc tacttgtacg gagcttatcg ctgtttcaag tgggctgctt
     2881 ggagaaattt catcttgtgc tttaactagt tcagttgttc ccaagacaag accaacacca
     2941 atcaaaacag atgcaacccc aacacttaat tttttgatac tgtacttcaa tactcgtttc
     3001 tttgtttttt ctagaaatac tctcttatta ttcttgctaa ccattattca aatcaactcc
     3061 cttaataatt actttcctag taagaataaa agataaatct gatacgtatt attcacagaa
     3121 taggaatctt actaacctta tgttaactaa tttacttaga gaatttcatg aacattacaa
     3181 caaaaaaagt acatttcttc atgttttttt cggatatttg tgctttttaa ccattcagca
     3241 gctgaaaaca tataaaaaga tatgtatgta ttctttctga aattaagttc atttctatgc
     3301 gatgaagcat gttttcaaaa tattaccctt tcttagatta aacttcagag tattcaaata
     3361 acggaataag gagccttatc ccgcattact gaatgagact gtgacataag taaaaaggtg
     3421 acttcaaatt ccttcttcgg aatttgaaat caccttcttt ataattataa agtatcggtt
     3481 tcacagcatt tctgaatgat tcctagaatc ttaaacgaat gattggagtt atcaacattt
     3541 tttcactcgt ttttagcgaa acaaaaaatg acatttgtca cagcctcatt aaattattga
     3601 ttctttttct ttataattga attgtccata tttaaagtgt ctagtactct acatgaataa
     3661 ttaattgctt tcactcaaat acttataatc tattaacttg tagttgttta acttttctga
     3721 aaaagttaga cccacgtaat aatcttccca gctttctctt tcaattaact gatccaattt
     3781 ttgataatcc ataaattcat catgtatcaa aaagaataaa taacttgata ttttagcatc
     3841 tagcttaatc agattaaata atgtttgttt aaaattgcta ccattaatat gactgataga
     3901 ttctctagct tcttcacata aactttttac tttattaatt actactttgt catattcatt
     3961 taacacacta ttattaccat tacttagacg ctcagattct acgaaacaac tcaaatcaga
     4021 aatttcccaa aaaaattttt tgttaaaatc caatttcctc aactcctcca aactacgtaa
     4081 atgatatagt ctcataataa aataaaacct ctcagctaat gttgttgaaa ttgtccacta
     4141 aacctaattg atattgctta ggagatacac ctgcgtactt tttgaatact ctcgaaaagt
     4201 gtgaagaatc tgaaaatcct aaacctaaag aaacttcact gatagtatgg tttgatttca
     4261 acattatttt agccgtagaa atttttcgct tagagatata attataaatc gtaatatgca
     4321 tttcattctt aaagctttct cttaatttag tttcacttac agaaaaataa ttagctattt
     4381 cctttacagt aattctttta tagatattta agccgatgaa ttgaacgaca gaagaaatta
     4441 agggagaaag atgctctatc ttagtttttc cgatttcctt agtataataa acaatcccag
     4501 aatccctcac ttttaagacc tcaggtaacg atacagctaa ttcgttttct tgtatcaaag
     4561 catctctact ttgatacgct tctattatat ccattcctaa tgttatagac gtttgtgcta
     4621 acttttcaaa aacaatgata gaataatttt tttcagaccg aatcgtatct ccactaacac
     4681 ttggaattat tccattactc agattaaaaa ccatgttttc taaatttcga atatctccat
     4741 ttttaacata ttccagtatt ttattttcat aaagaaacag atatatttct ggatcgtaat
     4801 tttgtgataa cagattatct attttgattt gctcgatagt tttacttaga ttttctgtat
     4861 attcaattaa cggttcatgg aatagatctt ctatttttcc agtaaaaaaa taatgaacta
     4921 aaatcagaat atctcgaatg tctcctaaag aatacaaagt caaattttgc aatgaattat
     4981 agagtcgttc tctatcagcc attgcaacat tcaaattgtt catttttagt ttaaaaaaaa
     5041 ctttatcaat attatttgct ctaaatggac caaataagta atatctggtt ttaaacatgt
     5101 atagaagaaa catctcatta aaatcaccag taaagaaatg gaaatcttta gtttttttaa
     5161 tattatcagt tagtaacttt ttataatcat aaaaaaaata agtagttcta tctgagcgat
     5221 actcttttat aatactgaat tcggaattaa aaaccacgat aggtaacttt aaacaaacat
     5281 gaagagactt taataagact tctttatctg aaaacacttt tgtttctctc tttcctttct
     5341 acatcattaa atagtatttt atacgttatc taaaatacta ttcctctgtc aaaataagct
     5401 aacttacttc attataaaat atccacttat cgctttcatg tatattccac cgtaaaaagt
     5461 acattttatc accgagaact catcattttt catcatgatg aatactattt acatatatct
     5521 agttcaaaga caataattat tatgatccgt tattatctaa gcactataca gataatagga
     5581 atttttcgtt tctaaatatt ccaatttggg aatccaataa aaattttcgt tcaaatacgt
     5641 gtctattcga actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat
     5701 ctatacacga ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa
     5761 aaagctcaag acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa
     5821 ttttcttttc taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa
     5881 taaacatcag cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat
     5941 aattgcatta gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga
     6001 ggtgtgcaat atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga
     6061 aaaggtcatt ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca
     6121 tgaacaactt gatcaactcc caccatattt gtttactaaa gatactataa aatttaaaca
     6181 accgattaat gttttaaatg tgactctcct tgttgaagat ataaaattag caaaaatcct
     6241 tcctttgttg aaagaacctg aacaaatttt tcttatcgaa aaatttatct ttgataaaac
     6301 tgataaagaa attggaaagg ttctggggat tacaagacag ggtgcgacaa atttaaaaca
     6361 tcgactttac aaaaaaattc aaaaaatgct ggtttaatat ctgcataata actaattttg
     6421 tatgcttctt tgctcgttct cctccttccc aaagaaatag attataagtc catgggaaga
     6481 aggtagattt atgaattctt taaaagaaga tttcatttta gcaaaggctg gaaatgaaga
     6541 agctgttgag gcaattctca aaagatttag ctctttgatt cataagcaat cctggagaac
     6601 tggaaaatat gatcaagatt gttatcaaga atgtatgctt gcaatatatc tagctatctc
     6661 gaaattcgaa attaaagaat aactcaacta agggagtctg tgacataagt aaaaagatag
     6721 tttcaaattt cgaacaataa atttgaaact atcttcttta taattataaa gtatcgattt
     6781 tacagcgttc tataatgatt catagaatct aaaacgaatg attttaatta tcaacgtttt
     6841 ttaattcgtt tttagcttaa aaataacttt tgtcacagtc tcactcctaa aattacttta
     6901 tttacgtatg aagtgggcag gaggtcgaat ccagaactgg gctatggtac ttcaacgagg
     6961 acttctcctc tagaattgaa aagcatgcga tttaccttcc gtaacgacgg tagatttatt
     7021 caactatgtg tccaatcaaa atttgtctga tttcaaaaat agtcaagaga attttggttg
     7081 ttaaaaactc ttatctatca acttccacag tttagttgac aaaccctgat actaagttgg
     7141 gctatgatac acttttcttg actatcagtt acgttaccgg aaggaatgaa ctctattgag
     7201 aaaaacatat gatcgtaaat ttaaactaaa gatttcacag gatatccttg agaaaaaagt
     7261 aactaccaag aaaattgctg aagaatacaa tatctctcgt ccaacaattt cccgatgggt
     7321 ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga caaggaaaaa gattacctga
     7381 taaagcagat ttctacattc ttgaacagga aaataaacga ctaaaagagg aagacgagtg
     7441 ttctacgaac acaaagatcg atacggatct ccagggatca ctcaaaaact ttacgatgaa
     7501 ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc gtgaaatgaa tttatgcaca
     7561 agaggatttc atcgacgcaa atcttctcat ggtagagaaa aaacaatcaa agaaatggta
     7621 aaaaaatgtt gaatcgataa tttgaacaga accaaattga tgctgtttgg gtgatggata
     7681 ttacttacat tccatgtaat gacggtcgcc tctatttgtc cacctatatt gacttagcaa
     7741 ctcgtattct acgttactat atggtagatt cacacatgaa aaaagagatt gtcgtccgtc
     7801 cattacaaat ttattaagga gaattgccta cggtaattca ttccgataga ggtagccagc
     7861 atcggtcttt tatttatcaa gagctgttga acgaccacca catcacacat tcagtgagcc
     7921 aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt tcatcgatca atcaaatgag
     7981 aattaatcga ctccaacaaa tataaaagta aaatagagat gaaggtgtta atccaagatt
     8041 atttagaaga ctactaccca aacaaaagaa tccatacaaa atttatgatg actccttgac
     8101 aatttgaagc gaagaaaata agggatctat aatatctagt gttggtgacg ccttctgttg
     8161 ccaatacttt ttcttttttc taataaacgc aaacagacac cttaaaatcc tttagaataa
     8221 agtcgacgaa aaccattcaa aggagtgttt caagatgtcc tattcagagt ttaccaaaga
     8281 gttactagat attctagacc taaatcttat ctttcatgaa gatgcctttc gcaaagagcg
     8341 aatcaatgac gaaacatgtt ttgtttttga tggcacattg acctatcaac cggaggaatg
     8401 ctttcatcgc cactatcaaa atgaacaaac gatcattaaa t
//
LOCUS       DAZKOW010000116         8033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000116 DAZKOW010000000
VERSION     DAZKOW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.117"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            52..2829
                     /locus_tag="ACG912_002528"
     CDS             52..2829
                     /locus_tag="ACG912_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0513804.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            2910..3329
                     /locus_tag="ACG912_002529"
     CDS             2910..3329
                     /locus_tag="ACG912_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0513805.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            3341..3811
                     /locus_tag="ACG912_002530"
     CDS             3341..3811
                     /locus_tag="ACG912_002530"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0513806.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            3828..4607
                     /locus_tag="ACG912_002531"
     CDS             3828..4607
                     /locus_tag="ACG912_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0513807.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            4594..5415
                     /locus_tag="ACG912_002532"
     CDS             4594..5415
                     /locus_tag="ACG912_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0513808.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            5432..6481
                     /locus_tag="ACG912_002533"
     CDS             5432..6481
                     /locus_tag="ACG912_002533"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0513809.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            6494..7498
                     /locus_tag="ACG912_002534"
     CDS             6494..7498
                     /locus_tag="ACG912_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0513810.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
BASE COUNT         2661 a         1354 c         1685 g         2333 t
ORIGIN      
        1 tattaaagat tttattgaca aaatgaatcc gttttctatg atggaggagg ggtgaataag
       61 ttgttgaaga aggaaatttt atgttactta gagaaccaga cagccttttt cgatttagaa
      121 catatgaacg aggtctttac agcacaaagg ttagctgatc attttggggt aaaaagaaac
      181 acagtcagtc attatttgaa ccagttaaat gatgagcaac tactagtcaa aatcaattct
      241 cgaccagtga tttatttcca caaagaagct tttgaaaaac aattttttaa gttacgcacg
      301 aatttttatt tttctatcga tgatctcaaa gaggaacagc cttttttcgc gcaaccaaaa
      361 gacttattct cttttatgat cggatacgac gccagtttga aagaaagtat cgaacaaatc
      421 aaaacagctc tttactatcc agatggcgga ttaccacttt tgattacagg cgaaagtggc
      481 acaggtaaaa gttatttagt taatttggtc tatcagtatt gtctgcttca tgatctgcta
      541 gaagattctg caccattcat cacttttaat tgtgcacaat atgcagataa tcctgaactt
      601 ttgactagca atttgtttgg acatgttaag ggagcgtaca ctggtgcaga agaaaataaa
      661 aaaggggctt ttgaagcagc agatggtggg atcttgtttt tagacgaggt acatcgctta
      721 acaccagaag gtcaggaaaa actgttcacc tatcttgatc aaggtgtgat ttatcgtatg
      781 ggagaaccta atttgtcacg ccggatcaaa acacgtctct tttttgctac gactgaagag
      841 attactagtc atttcttaac aactttcatt cgaagaatac ctatccgaat cgaactacct
      901 gcacttaatc agcgtagtcg taatgaacgg ctagaactga tttactcttt ttttctcgaa
      961 gaacaaagaa aaatggggag ccctttgtca gtcagtggac cagctttgtc acttttaaca
     1021 agtcagaaat tatccggaaa cataggagag ctgaaaaata tcgtcaaagt ttcagcagca
     1081 aaagcatatg ctgaacagcg tgaacagcct gaaatccatg tcacgatcca tcatttgcaa
     1141 aaagaactac ttgcgcagcc tattactaaa aatacagcac agcaaggaat ctatattacg
     1201 caagaccaga ccttagagca attaatggag aagcaacagc ccgaacagca gcgaatcgtt
     1261 aaaagttttg agcaaatcct aattgatttc aagaaaaata actgtctgct atattcttgc
     1321 gaaggaaaat taaagcaaga agtgattcaa ttatttgatt ttttgctgtt tgagaccagt
     1381 agacaagaaa aacacgagct gctggtttat tggacacaat ctatccgaga gacctttcga
     1441 caaatggaaa ctgcctatca aatcaaattc gatggaaaca gtgtctatgc cttgagttac
     1501 tatctctatc agcgaagcac tatcaaatgg ttaccggaag aacatgatat tatccaacta
     1561 atcaaagaat tagaacaaca agtttctcag tcttatccat ctagttatca ctatgtccaa
     1621 cgaattctgg aattaagtaa gatcaaatta gatattgaag acacaagtat ggatcggatt
     1681 ttactgacga tctatctaaa aaaagcgaaa tggtcaaaag aaaaacgact gccaaaagcg
     1741 atgattgctg ctcacggtta tgcaactgct agcagtatgg caaatgtagt aaatcgttta
     1801 ttacaagagg atctatttga atcatttgat atgccactag atgtcacacc acagcagatt
     1861 gcttcagaaa tccttgatta ttgcgaaaac agcgatgttt caaatggatt gatcatatta
     1921 gtagacatgg gatctctgaa agaaatccat cagtttttta aaaaacagtt atccgtaccg
     1981 cttttgattt tgaataatgt gacgacacct ttagcaatca cagtagggga atgcctgcaa
     2041 aaaaatgtat cattagaaga gatagcagaa gagactgttc gacagatcca gccagaatgg
     2101 cgactgctct atccagagga aaacaaaccc aaagcactca ttactacttg cttcactgga
     2161 atcggtacgg cggtccactt aagtgaactg ctagaaaaaa gcttacctac ggcttgtcag
     2221 ttgaaaatca tcccatatga atatcagcaa ttaaaagata agaaaaacag tgacccatta
     2281 ttttccatat acgaagtcgt aggaatgatt ggcaccacag atcctgatat caccaatatc
     2341 gactatcttt cattagaaga cctcatctcg ggggaaaaga tgtctgtact agctgaatgg
     2401 ttggacacta ctatgaatgc ttcagagaag gaaatattca atcagcgcat cattcgtaat
     2461 ttttcattgg aaaaagtact ggattccgtg actattctag atacagagaa ggtgatggga
     2521 gaaatcgatc attttatgag agaattagag gtgcagttga atcgaagcat cagcaatccc
     2581 aaaaaattag cgttgtatgt ccatgtgagc tgtctcattg aacgattgat ccgacaaatg
     2641 ccaatcgaaa cctatcaagg attagagaaa tggaagcagt gtcaaaaaga agggttatct
     2701 gctattaaat ctgcgtttag tgtcattgaa gagcattata gtgtcatgat ccccaattct
     2761 gagattgcct atatctatga tattttgagt gaagatacag agtttctctc gatagaagaa
     2821 gaattttgaa cataagcaag ttgttgccac taattttggc atgcaacttg cttttttagt
     2881 atgtgtaagt gaaaagagga gatgaaaaag tggatagaga aattatctta gcttcccatg
     2941 gaaaatttgc ttcagggata ttagattcat tagagctgat ctatgggaaa aatcattcta
     3001 ttactgtatt cgattgctat actgatgaga attttgattt agcggaaaca gtaaggcagc
     3061 tttttatgag gtacaaagat aaagaaatca ttgttctgac ggatcttttt ggcggaagtg
     3121 tcaacaacga attcttacaa tatatcaatc gggatcatgt ctatctcgtt gccggattga
     3181 atttgccact attgattgag ctagtatctc ggttggacgc tgaagtagag acaactgttt
     3241 tgatccagca ggtgctaact gattcaaaaa aaatgatcca gttttgtaac gagcggctca
     3301 atcagaaaat agaagaagac gaattttagg aggaagaaaa atgattgtat tgacaagagt
     3361 cgaccatcgg ttattacacg gacaagtagc tttttcatgg acacaaacaa taggcgcaga
     3421 ctgtattttg attgctaatg atgatgtacc tacgaatgag atacgaaaga caacgatcaa
     3481 attagccaag ccacaaggag tcaaactagt gatcaaatca attgatgact cgattgccgc
     3541 gttgaaaagc ggggtgacag ataaatataa gttattcatc gtagtggaat cgatcgagga
     3601 tgcatataag ctggcagaag cgtatccaga aatcaaaacc atcaacttag gagggatcaa
     3661 ggcaaaacct ggtactcgca gcatcggaaa agcggtaaat attttaccag aagaggagga
     3721 acgcttgaaa cgattaattg acaaacaaat agaagtagaa atccgtcaag taccagcaga
     3781 taaaaaagtg gatgtaagag aagttttgta agaaaggaag aacctcaatg attgtacaag
     3841 cagtcttatt aggaattgtt gcatttgtcg cacaatcaga atatgcacta gggacatcgt
     3901 tgctttctcg tccgattgtg acggggctgt ttacaggaat cgtgttaggt gatgtaaaaa
     3961 ctgggatcat tatgggcgca actttagagt tagcatttat cggatcgttc tcagtaggtg
     4021 cttcgattcc acctgatgtt gtgaccggtg ggattttagg gacggctttt gctattacag
     4081 ctggagccgg tacagaaacc gcgcttttac taggtttgcc gattgctacg ttgacattga
     4141 tattgaaaaa tatttactta gggcttttga ttccaattat gaaccataaa gcagacaatt
     4201 atgcagaaga aggaaattat aaaggagtcg agcatatgca cttgcttgcc ggtatcggtc
     4261 tttccttgat gcttggtgtt gtggtaatgg tttcttatat ggtgggaagc aatacgatcg
     4321 gaaatctatt aaatatgatt cctgacttcg ttcaaaaggg gttgtcagta gctacagggt
     4381 tgattccagc tcttggtttt gcgatgcttg ctcgtttgct gatcaacaaa caagttgcac
     4441 catatttttt cttaggtttt gctatagctt cttacttaga gattcctgtg accggagtag
     4501 ctatttttgg tgcgatttta gcagttgtgg tagtcaatat tatgaatgtc cgtcaactac
     4561 cacttcaaac aactgcaggg gaggtagaag aagatgaaga attctgagat caaaaacagc
     4621 aaaataacga aaaaagagct aaatcacgta ttttggcgtt cttttcaaat ggagttttct
     4681 tggaattatg aacgtcagat gaatatggct tatgtttatg caatgattcc gattttgaaa
     4741 aagttatatc agtcgaaaga agaaatggca gcagctttga aacggcatct agagtttttt
     4801 aacacgaccc cacatatcgt tacactgatt ttaggaatca cggctgcaat ggaggaagaa
     4861 aataaggatg atcctgaatt tgatgtgact gcgatcgata gtatcaaaac atctttgatg
     4921 ggacctcttg cagggttagg agattctttc ttttggggaa ctttgcgatt gattgccaca
     4981 ggtgtgggta cctctttagc catgcaagga aatattttag gccctatctt gttcattttg
     5041 attttcaata ttccgcacat tctgtttcgc tatctagcaa caggttgggg atatcgctta
     5101 ggaacgggtt ttttgaaaaa gatccaggca aacggtatga tggaaagttt gacattaggc
     5161 gcgtctatta ttggactgat ggtcgttgga gcaatgactg ccacgatgat caccatcaat
     5221 attcctttaa aaatcggttc aggagaaaat gctgtgactg ttcaaagtat ttttgatgat
     5281 atcgttccaa atatcttatg tcttggagca tttggcatag tgttttatct attgaagaaa
     5341 gaagtgaaac cattaacgat cttgatcgga ctagctttct ttggaatttt tggttcgtgg
     5401 atagggattt tttaaaaaag gagcagagaa aatgtttacg atgcaagatt atatctatga
     5461 agaaaaggaa gtactaagta cgatcttgaa aaagaatgat ttttcgacaa gagaacatat
     5521 gaaaaaaaca gtgaatttac tgatcttagc aacgggttca tcttataacg cttgtttagc
     5581 agcgaagcca gcattggaat cttatggtga tcttactgtc gatattcaag aaccttttta
     5641 ttttttcaat tatggaaaac tttctccttc aatcgatact gtgattgctg tgtcacaaag
     5701 cgggaaaagt gcttctactg ttgaagcagt aaaaatgata caaaaacaag ggttacctgt
     5761 tgttgctatc acaaatgacg tgcaaagtcc acttgcttta gaagctgcac aaattattga
     5821 tttaggtgcc ggggtagaat cagtaggctt cgttacaaaa ggctattctg cgactgtttt
     5881 acagttacta ctcttaggga taggaattgg aataagtaaa aataaaatca gcaaaaaaat
     5941 agaacaagac tacatgcaac agttgcgcaa aataatcaac catttaccag cgattattca
     6001 aaaaacggaa gaattttttg atggatatca aagtttattt cgtttagcac agcgtttcgt
     6061 caccattgga tatggtccaa actggggaac agctaaagaa gcagagacga aattgactga
     6121 aacaatccgc gttccgtcac aaggatttga gttagaagct tatatgcacg gcccatacct
     6181 ggaggcagat gcttcgcatt tgctattctt tattgaaggg gaaagtgtaa ataaggaacg
     6241 ttcgcaaaag ttgcaaagat atatgagtcg gtacgttgga gagactctca ccattaccac
     6301 aaaaaaagca agaaacgaga aaacacttgg attagcaata gaatgtgatg aatatctgtc
     6361 tgtattagcg cttgtcgttc cgtttcaact attcgcttat aaaaccgcag tagctaaagg
     6421 tattgacttg aacaaaaaga tttttgaaga ctttgataca gtcttgaaaa gtaaattata
     6481 agaggtggaa atcatgctga aattcaatga agaagaacaa atcaaagcaa taaaaggcgc
     6541 actagcttta cgtccgcaag tagaagaaat catcgataaa ctttatgcgg aaaaatttga
     6601 cgctgtctat tatctaggaa tcggtggaac gtatgcttcc tcgatgcaag ctgtcactta
     6661 tatgaatggg aaaagcaact tgcctgtctt tgtacagcat gctgcagaat attacactac
     6721 tggaaacaaa agattaacaa aagattctat cgttattttg tcctctgtta ccggtacaac
     6781 tcaagaagta gtgaaagcag tagaacaaat caagaaagta ggagcgacac tgattggatt
     6841 tatcgataaa gcgaacagta agttgtctca gctatgcgat tttgtggtca cttatccagc
     6901 tcctggaaca gaacaaatca aattttttat ggcagcagat cgattgatgt atttgaatgg
     6961 agaattcgaa gcttattcag aatactatga acagctcgaa cgttatttac caacagggtt
     7021 agtggaagca gagaaaaaag cagatgcatt tggcttatca tttgcagaaa aacatcgacg
     7081 tgatagtatg cattatttta ttggagcagg gaatcaatgg ggagctgttt attcatatgc
     7141 catgtgttac tgggaagaac aaagctggct tccatcgaaa tctatccatg ctgccgagtt
     7201 tcttcacggg acgttagaga tcgtagaaga aacaacccca gtcacattat ttttaggcga
     7261 agatgaacag agagtgttat ctgaacgtgt ggctaaactt ttgccaaaaa tttgcagcaa
     7321 ttatacattg atagacacaa aagactatcc agtagaaggt atcagtgaaa aatatcgcgg
     7381 acgtgttttg tctttcttat tgatgcatgt agtgacacaa cgcatcgatg ctcatgtgga
     7441 gcagttgaac tgccatccat tagagatacg ccgctactat cgtcagtttg attattagga
     7501 tagactggat aaaaagaata taaaaaataa agagagcagc gataactgct ccctttattt
     7561 ttttaaaata ttggcatcaa ttacgttata aaacacattt tttgcattat ctacagttca
     7621 acttataatc tgtttaatgg gacatgtggg tgccaaccat tttttgttat gcaatagtac
     7681 tatttagttg ttgcatggat ccgtagtaaa agtccaagta caacgagttg cgccagttta
     7741 catagcgttt tgtatccaga atgttgacta aaacaaaagt tcctatgtca accgtcaaca
     7801 gagacaatct gtccgttgca ggacctgaca gatttagaaa tgttcaatct gtctcttatg
     7861 tattttaaat caatactcac taggagaagt ttcctaacct cctaatagta atgctggtag
     7921 taacattgtc aaaactgctt cctcttagga acaagcaaag cataacgagt gtaaaaataa
     7981 aatgtagact attgtaacat atcattgatt atttcaagta aaaacgataa aag
//
LOCUS       DAZKOW010000117         7599 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000117 DAZKOW010000000
VERSION     DAZKOW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.119"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..426)
                     /locus_tag="ACG912_002535"
     CDS             complement(136..426)
                     /locus_tag="ACG912_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513811.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(544..804)
                     /locus_tag="ACG912_002536"
     CDS             complement(544..804)
                     /locus_tag="ACG912_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513812.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(941..1354)
                     /locus_tag="ACG912_002537"
     CDS             complement(941..1354)
                     /locus_tag="ACG912_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFQ0513813.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="ACG912_002538"
     CDS             complement(1810..2295)
                     /locus_tag="ACG912_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513814.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="ACG912_002539"
     CDS             complement(2434..2649)
                     /locus_tag="ACG912_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFQ0513815.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="ACG912_002540"
     CDS             2873..3673
                     /locus_tag="ACG912_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0513816.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="ACG912_002541"
     CDS             3692..5560
                     /locus_tag="ACG912_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFQ0513817.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="ACG912_002542"
     CDS             5553..6044
                     /locus_tag="ACG912_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFQ0513818.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /locus_tag="ACG912_002543"
     CDS             6032..6586
                     /locus_tag="ACG912_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFQ0513819.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..7599
                     /locus_tag="ACG912_002544"
     CDS             6604..7599
                     /locus_tag="ACG912_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFQ0513820.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1443 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatacgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctga
//
LOCUS       DAZKOW010000118         7537 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000118 DAZKOW010000000
VERSION     DAZKOW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.120"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            107..1248
                     /locus_tag="ACG912_002545"
     CDS             join(107..335,335..1248)
                     /locus_tag="ACG912_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0513821.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="ACG912_002546"
     CDS             1483..1935
                     /locus_tag="ACG912_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513822.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="ACG912_002547"
     CDS             1949..2638
                     /locus_tag="ACG912_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513823.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="ACG912_002548"
     CDS             2763..5114
                     /locus_tag="ACG912_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFQ0513824.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="ACG912_002549"
     CDS             5194..5589
                     /locus_tag="ACG912_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513825.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="ACG912_002550"
     CDS             5914..6531
                     /locus_tag="ACG912_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0513826.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="ACG912_002551"
     CDS             6572..6871
                     /locus_tag="ACG912_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFQ0513827.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="ACG912_002552"
     CDS             6905..7072
                     /locus_tag="ACG912_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFQ0513828.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="ACG912_002553"
     CDS             complement(7117..>7537)
                     /locus_tag="ACG912_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0513829.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DAZKOW010000119         7217 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000119 DAZKOW010000000
VERSION     DAZKOW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.121"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG912_002554"
     CDS             complement(229..906)
                     /gene="phoU"
                     /locus_tag="ACG912_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFQ0513830.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG912_002555"
     CDS             complement(919..1677)
                     /gene="pstB"
                     /locus_tag="ACG912_002555"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0513831.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG912_002556"
     CDS             complement(1693..2499)
                     /gene="pstB"
                     /locus_tag="ACG912_002556"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFQ0513832.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG912_002557"
     CDS             complement(2514..3398)
                     /gene="pstA"
                     /locus_tag="ACG912_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFQ0513833.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG912_002558"
     CDS             complement(3398..4318)
                     /gene="pstC"
                     /locus_tag="ACG912_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFQ0513834.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            complement(4433..5290)
                     /locus_tag="ACG912_002559"
     CDS             complement(4433..5290)
                     /locus_tag="ACG912_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFQ0513835.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG912_002560"
     CDS             complement(5627..7075)
                     /gene="cls"
                     /locus_tag="ACG912_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFQ0513836.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT         2093 a         1589 c         1255 g         2280 t
ORIGIN      
        1 ttgcattctt tatcctttct atgaaatgat gaaaagggtt tgtgacatgt tttgtcacaa
       61 accctttccc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaaa
      121 cgaaaaatat gaggagctat tttttgtttg actgttctta ttacttgatg tagaacagct
      181 ttttcacaac ctcttttcat aaacaaacgg cataatcaca gcttcagttt agaaaatctc
      241 attgtggttc gtatttaact cagtgatttt ccctgtagcc aagtagacga tccattcaca
      301 gatgtttgtc acatagtctc cgatccgttc tagataactg gcaacattca agtagtctgt
      361 tccactgatg actgtctcag gattttcctg catatttttg attgttgcat tatagattcg
      421 attgaaatag tcattgactc tttgatccat attggcgatc gtacgtgcgt ctttttcgtc
      481 tgttttgaca taagcaacta ggacgttgtc caccattttt ttcacataat cggacatatc
      541 ggagatctct ttttcgattt ccggtattct cgtctgccct tttacgcgaa tcgtggattt
      601 agcaacagac accgcatgat cggccatacg ttctaaatcc gagctagctt tcatgaccgt
      661 aatgatcata cgcaaatcag ttgtaactgg ctgttgcaat gcgatcattt caaaactttt
      721 cttctccaag cgtgtttcca tgttattgat atttatatcg ttttcgataa cttcttgggc
      781 taagatttta tcgtgtttga tgtatgcacg aactgacttg tgcacggcgc tgctcaccat
      841 catccccatt tcgtagaact gattatggag attcagcaat tcttcttcaa attgtgtacg
      901 tagcattccg tttcctcctt atccaaatcg acctgaaata taatcttctg tctcttgttt
      961 ttctggattc atgaatatct tcttcgtatc gttgtattcg atcaagtgac catctaaaaa
     1021 gaatgccgtc ttatctgaaa tacgtgaagc ttgagacata ttatgggtca cgatgatcat
     1081 cgtataccgc tctcttaatt ctaacagcat actttcgatt ttcccagtag aaacaggatc
     1141 aagcgcactt gttggttcat ccaataagat gatttccgga ttaactgcca aaacacgtgc
     1201 gatacataca cgctgctgct gcccgccgga taaagataaa gcacttttat gcaatttgtc
     1261 tttcacatca tcccaaacag acgctgcctt caggctttct tcaactactt gatcaaggac
     1321 ttttttatct ttttcccctt ttaatttcaa tccatagatg acattttcat agatagaaaa
     1381 aggaaatggg tttggctgct ggaataccat gccgatttct tttcttagtt caacaacatc
     1441 tgtttttgga ccatagatgt ctttgtcttt atataaaaca cttccggtga tcgttacatt
     1501 cggaatcagg tcattcatcc ggttcaaaga acgcagataa gtggattttc cgcaaccaga
     1561 aggaccgatc atcgcagtga tttccccttg attgaaactt agatcgatcc cttttagtgc
     1621 ttctttcttt ccataataca gatgaagatc tttagatgaa ataatttctt ttgtcatgtt
     1681 gaggcctccg tcttaaccaa aatgaccgga cacataatct tcggttgcct gaatttttgg
     1741 tctggtaaat atcttgttag ttttgtcata ttccattact tttcctagat agaaaaaggc
     1801 agtatagtcg ctgatacgag ctgcttgttg catgttatgc gtcacgatga tgatcgtata
     1861 gtcttctttc aagtttacaa gtgtttcttc aaccgttcct gtcgaaatag ggtccaaagc
     1921 acttgccggc tcgtctagca gcaagatatc aggtttcatc gcgatcgcac gagcaatgca
     1981 taaacgttgc tgttgcccgc cagataaagc caaggcactt ttgtccaagc tgtcttttac
     2041 ttggtcccac aaggcagctt gtttcaagct tgtttcaacg atttcatcta atttcttttt
     2101 gtttttttcg ccgtgctgtt tcaaagcaaa tgtaatattg tcataaatcg acttgctgaa
     2161 cggattagga cgttgaaaaa ccatgccaat ccgtttgcgc atctcataaa catccacttg
     2221 aggcgcatta atatctacgc cttgatacat gattttccca gtgattttcg tattagcgat
     2281 cccatcattc attcgattca acgaacgaag atacgtagat tttccgcagc cggaaggacc
     2341 aatcagtgca gtgatcttat ttttttcaaa ttccagatcc actcctttga ttgcttcatt
     2401 gtcgccatac cagacatgta tatcatccgt atatagtgct acttctttct cttttggcat
     2461 ggtgatgaca tttgtttcat tccagttata ttttttcatt ttgcttcttc tccttaggct
     2521 gaagtcattt ttcggtataa tcgtttgcca atcgcacgag cgccgaagtt gaacagtaaa
     2581 acagcaatga tcaacacggc agaagcgcca gccgaaacag aaacaccatc aggcatcgtg
     2641 ccttctgtat tgatcttcca aatatggaca gctaatgttt cagcttgacg gaaaatgctg
     2701 atcgggcttg aaacacttaa tggattccag ttcgtaaaat ctaacgcagg tgcactttgt
     2761 ccagccgtat agatcaatgc agctgcttca ccaaaaattc ttccagaact taggatcaca
     2821 ccagtcaaaa tccctggtgt cgcttctgga accaccactt tcatcactgt ttcccagcgt
     2881 gacaaaccta aggccaatcc tgcttctcgt tgtgtgtaat ggaccgcttg taaagactcc
     2941 tcaactgttc tagtcaaaag aggcaagttg aatacagtca aagccaatgc accagaaata
     3001 atcgagaaac catactggaa ttgaataacg aaaaccaaga aaccgaataa accgacaacg
     3061 acagaaggca acgagctcaa gatttcaatc gacgtgcgaa tcacatctgt tagccagttt
     3121 tttccagcat attctgaaag atagataccc gcacctaaag aaataggaaa actgatgatc
     3181 atcgtgatca aaagcaaata aatcgagtta aatagctgga tcccgattcc accaccttct
     3241 tggtaggctt tggaaggttc ggtcaagaag gaccatgaaa tgtgcggtat tccccgaact
     3301 aagatataaa gtagtaaagc tgctaaaatc aatacgatga tccccgaaac tatatataaa
     3361 acacctgtag caattttatc tgagcgtttt gcgttcatta tttcagctct cctttcttcc
     3421 caatcatacg gatcacgata ttaaagaata atgacatcaa tagcaagatc agcgctaatg
     3481 accataacac attattttca actgttccca tgattgtatt tccgattccc attgttaaga
     3541 tggacgtcaa tgtagaagca ggagtagtaa gtcctgttgg catcaatgct gcattaccga
     3601 tgaccatttg gatagctaag gcttcaccaa acgcacgagc cataccaaaa acgactgctg
     3661 ttaaaatccc gggcacggct gctcgtagca cgactttgta gattgtttgc catcttgtgg
     3721 caccaagcgc taatgaagct tcccgatagt gccgtggtac tgcctttaat gcatcaacag
     3781 tcatcgttgt gacagtcggc aagatcataa cgaacaacac aaatgtacca gctaagatac
     3841 caaaccctgt cccgccaaaa atacttctga caaaaggcac gataactgat aaaccgataa
     3901 aaccataaac gacagaagga atacctacaa gcagctcgat gaccggctgt aagatcttcg
     3961 ttccgttttt aggagagatc tccgtcatga atactgctgc accaatggca aatggcgtgg
     4021 caacgatagc agataaaaag gtcacaataa acgaacctgt gatcataggc aatgccccta
     4081 ccattggttt cccatttgct ccgatttggc ttggattcca atctgtacca aacagaaaat
     4141 caaatacatt gatcttatca gtaaaaaagg tggccaatcc tttactagct acaaaataaa
     4201 aaatagataa tacaaccaac acgatcagcg cgatacataa aaaactgatg aattttccac
     4261 gctgttccat ttttgaacgt tttgattttg ttaataatac ttttttcaca tcttccacag
     4321 atgactcccc cttaatcagg tttaacatac tcactcagtg tacaaattga acatcaattt
     4381 ggttttaata acatgtaaaa gtttagctaa gtttgtaaat tttgtgtaaa gattactctt
     4441 taacgatatt tccttgccaa tcacgttcga ctttcatatg agaaacaggg atataaccta
     4501 gttctccaac gatgttatct tgtacttcat cagataagat atagtctaaa aattcttttg
     4561 tcagtttttt tggatgatct cttgtgtaca tatgctcata tgcccaaatc gtccatttgt
     4621 tcgttgtcac attttcatct gtcggttcta caccgttaac attcaacgta tctacatcat
     4681 cggaaacata agaaaaagca acatagctga ttgcaccggg tgtgtcggca acgatctggc
     4741 gcaccattcc gcttgaatct tgttcttgtg cctgaacagc tgtttctccg tccaataccc
     4801 atttttcaaa cgtgctacgg gttccgcttc ctgaagcgcg gttcaatata actactggct
     4861 gatcttttcc accgacttca ctccagtttt tgatctcgcc tgtaaatagt tttttcaggt
     4921 tttccatact gatgtctttt acgccaatat ccttgttcac aataggagtg atcccaacaa
     4981 ctgctactcg atgatcgact aatgctgagg catcgatccc cgatttttct tccgcaaaca
     5041 aatcagagtt tccgatgtcg actgctcctg actgtacttg agacaaacca gttccgcttc
     5101 cgcccccttg tacattgatg aattttccag gattttctgt ctggtaagtt tcagcaactg
     5161 tttctactaa aggctgtaaa gcagaagagc caacagccgt aatggattct cctcgatcaa
     5221 tccactttga acagcctgat aaaagaatgg ctaatcctat tattggtaag aataatttaa
     5281 gttttttcat tagaaatctc ctttaatcct aaagtatgtt tattttatca ttttcattac
     5341 ttgatgaaaa cgaatcaaaa tcattaaata acaaaataaa ttctcataat tgtaaaaaaa
     5401 actatctcat taaaagtttc tatgctatac tggctttgaa atgtgtctct tttaatagca
     5461 tatattagct tatttcttgc tttccccaaa aaacaagaga taccccaaag ttaatttcgc
     5521 tcatgcgaga cgctttctcc atttctgacg tactctcaga aatggccccg ttagaaaagg
     5581 acacgagatc tccatctgtt gatggttctc atgtcctttt tatgttttaa aggagcggcg
     5641 aaatcaatct tgctaatcct tctttcatct tgatgattat tcctcggttt tcatacattt
     5701 cttttgtgag atgcgtagaa attttcgagt cttcaaaaaa agcttttctt acacgtgaag
     5761 ccattcgttc atcataaacc agtgtattga cctcaaagtc caagcgaaat gaacgaacat
     5821 cgatatttgc tgagccaact gaaacaatgc caccatcgat aatcatcgtc tttgcatgaa
     5881 taaatccatt ctcatatgtt tcgatccttg ctccatactc gatcagctct gcagcgaaag
     5941 aatacgttgc ccagtaaacg agaagatgat ctggtttgtt aggtatttgg atatgaactt
     6001 ttacgcctga aagaagagcc aacttcagtg cttcatgaat cgatccatcc ggtatgtaat
     6061 aaggcgtttg gatcaatatc tcccgtttgg ctaacgaaat cattttcaaa taagtcattt
     6121 tgatttgttc atgttcggta tctggtccgc ttgtcacgat ttgaacagct atatcaccag
     6181 tagtgtgaat ggacgggaaa aatttgggag aatagcctac ttcttttgta tgctgagaat
     6241 tccagtccat caaaaaacgg ttttgcaagg tatacaccgc ttctccgtaa atacgcaaat
     6301 gattgtctcg ccaataacca aatttcttta ccaatcctag atattcattt ccaacattga
     6361 acccacctgt atatccgatt ttcccatcaa taactacgat tttacgatga ttgcgataat
     6421 tgatccgagg attaatatac ggaacaaata gaggaaagaa aaatgcgact tctccgcctg
     6481 cttttttcaa attctgaaaa aagctggaag aaacttgtgt ggaaccccat gcatcaagaa
     6541 ggactctgac ttttacccct cgtcttgccg cctctgtcaa tgcatctcga acttcttcac
     6601 ctaatgcatc actgcgataa atataatact ggaagtggat atggtcttct gcctgcttga
     6661 tatcttcaat cagcgcatcg aatttttcac gaccatctgt aaataaagtg atttcatttt
     6721 tagtcgtata gagcgtactt tcaaataccg tgagcatata gatcaattgt ttcacatcta
     6781 cttgtccagt aggcggatga ggataaagcc ctcgcatcaa tgcctgtttg tcttcttcaa
     6841 tttccaggtt cttaccgatt ttcccttgga tcttcaaatc aaagattttc tctttggaaa
     6901 tccctcttcc taaaaaaata tataaaataa atcctacaac tggaatgaac atcaaaacga
     6961 gcagccatgc ccatgtttga gcagtttcct ttctttctcg aaaaacgatg ataaaggaaa
     7021 gcaaaatatt cataatcaaa aggatcgtaa aaaaattgtc aacgataatt ttcacggctt
     7081 cacctcttct tctaccaact taagaatacc caagttgttt tttaaagtaa agggaaaaaa
     7141 gccgcttcct tctattctcc tagccaataa aaaaagaaag ctggaacgaa actgtccaac
     7201 tttctcattt tatttat
//
LOCUS       DAZKOW010000120         7027 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000120 DAZKOW010000000
VERSION     DAZKOW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.122"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            179..1036
                     /locus_tag="ACG912_002561"
     CDS             179..1036
                     /locus_tag="ACG912_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0513837.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1029..1931
                     /locus_tag="ACG912_002562"
     CDS             1029..1931
                     /locus_tag="ACG912_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFQ0513838.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            1964..2314
                     /locus_tag="ACG912_002563"
     CDS             1964..2314
                     /locus_tag="ACG912_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0513839.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2363..3286
                     /locus_tag="ACG912_002564"
     CDS             2363..3286
                     /locus_tag="ACG912_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFQ0513840.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3364..4257
                     /locus_tag="ACG912_002565"
     CDS             3364..4257
                     /locus_tag="ACG912_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0513841.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4295..4858
                     /gene="amaP"
                     /locus_tag="ACG912_002566"
     CDS             4295..4858
                     /gene="amaP"
                     /locus_tag="ACG912_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0513842.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4872..5060
                     /locus_tag="ACG912_002567"
     CDS             4872..5060
                     /locus_tag="ACG912_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0513843.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5073..5975
                     /locus_tag="ACG912_002568"
     CDS             5073..5975
                     /locus_tag="ACG912_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0513844.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6007..6249
                     /locus_tag="ACG912_002569"
     CDS             6007..6249
                     /locus_tag="ACG912_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0513845.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6505..6876)
                     /locus_tag="ACG912_002570"
     CDS             complement(6505..6876)
                     /locus_tag="ACG912_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513846.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2568 a         1189 c         1394 g         1876 t
ORIGIN      
        1 atacctttga ataaaaagcc atgaaaaacg aatagttagc gcttaccgat gcgttttgtg
       61 ataaaaaaat tttaaaatat tcaatgaaaa caaagcatat attttgcttt aaaaccaaat
      121 gaggaatatt ctagatgtag aacaggttag aaaaagatta gaaaaaggag tgattttgat
      181 ggagaagaga tacggcgtag ttcctgtggt aactgcttca gacgaaaatt atgctcctta
      241 cttgagcgtg atgatcgcaa ctgcattaga aaattgcaat aaaacaagac gcataaaatt
      301 ttatgtgatt gacgatggtc tatcagaata tagtaaagaa ggattggaag aaacggtaaa
      361 taaatattca agtaatgcaa gtatccaatt cctgacagtg gaaaaagata tctacgaaga
      421 cttcctagtc agtgaccata tcacaactac cgcttactta agaatttctt taccaaactt
      481 attagctaaa gaggattata agaaagtcct ttacttagat tcagatgtgt tagtactaga
      541 cgatatcgta aaactatatg atgagccgtt aaatggaaaa acaatcggcg ctattatcga
      601 tccaggccaa gtgaaagctt tggaaagatt aggaatcgat tccgatgact tgtacttcaa
      661 ttcaggagtg atggtgattg acattgatca atggaataaa aaagaaatta cagaaaaaac
      721 tatccattac ctcagtgaaa atggcgatcg aatcatctat cacgaccaag atgctttgaa
      781 tgctgtactt tatgaagatt gggaacaact tcatccaaaa tggaacatgc agacttcgct
      841 gatttttgaa cgtcatccag caccaaatga aaaatatgaa cgtcagtata aagaaggaaa
      901 tgaaaaacct tcgatcgttc actttacagg acatgataaa ccttggaata cgttgaaaga
      961 tcatccgtat accaatttat acttaaaaaa attagcacac agtactctaa cgaaagtagg
     1021 cgagatcaat gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca aaattcgtac
     1081 cacatttagc tgcattattt gtctcagtat tagataattg caacccgtct aaatttgttc
     1141 ggttctatgt gattgacgat gatatcgatt ttgaaagtaa acagctttta cgcttctcag
     1201 tgaaaaacgc acgaatgaac tcagatgtgg aattcttgaa aatcaataaa gaattcttta
     1261 caaatgtggt aatcagcgat cgtattccag aaacagctta ttatcgtatt gcgattccgg
     1321 aattgttccg cggcacagaa gttgaacgta tcttatatat ggattgcgac atgatcgctt
     1381 tacaagatat ctctaaatta tggcgtcttg acttcggtga ttcaatcgta gcagctgttg
     1441 aagacgctgg gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa tcaatgcgtt
     1501 atttcaactc aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa aatattacgc
     1561 aaaaagtatt agatttcatc gaacataatc cagaaaaatt acgattccat gaccaagatg
     1621 cattaaatgc tatcttacat gaccgttggt taccacttca tccgcgttgg aatgcacaag
     1681 gctatatcat ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt gaatacgaag
     1741 aaactcgcaa caatccttac attatccatt tttcaggaca tgtgaaaccg tggagcaagg
     1801 attttgaagg gccaacgaag aaatattacg aaaaatatgc agggatgacg gctttccgtt
     1861 gtgtagctaa attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa acagatgtag
     1921 ttgttaaata atagaagaca acaactgaag agggggagta ggaatggaaa ttacaatgtc
     1981 taaaaatgca gtagagacgt taattgaaaa ggttggggaa aatacaaaaa tcgcactcgc
     2041 gcttatcaat gattccgatc catttctaag ggacaaaggt gcttttgcaa aaggcagctt
     2101 ttttcaaatc attccttttg tttcggaatt tggagaatat gcgactaaaa tcgagcatcc
     2161 attattggac atctatacat ctaaattaga gcaaaattat ttcggtaaac gattgaacat
     2221 ggacttcaac aaacagcttg acagcttttc attagaaaat gaaatcgcag tactcgatta
     2281 taatatcaaa ttaaagaact gcttcttcag ctaatagttt cgagaaagtc caaaaaggag
     2341 gtatacattg agatactact ttgtggatga agaaaagaat gtaatagaaa cagatgaaaa
     2401 aaaatataat tggctcgtac ttgatagtgc aaagcccgaa gagattgagc aagcaatcga
     2461 agaattcgag cttcctgatg atattttcgt aggaaccgaa tatccggaag aagtttctcg
     2521 gatggagcgc ctttatgaga cgaaactaga gcatccattt tctttggtag tgattaatct
     2581 agatcagtca gataatcgta tcgaaaaaaa attaacgcca atatcttttg tactttccga
     2641 taatctcttg attacttgta tcgatagaaa aaccgaattt gtggatcggc tgctgacaaa
     2701 gcatggaaat catttaaact cgtttgaaaa gatcatcacg tatacattat tagatattta
     2761 cacccattac gtgaaagaac taagagagat gaaaaagcga attgacgctt tggataaagc
     2821 agctcgtaaa acaacggaaa atgaagaact gtataaacag gcagatcttg aaagagacat
     2881 cgtatatatc gatcatacct taagagatca acgggaaaca atggatcaat tatggaattc
     2941 tgaaactttc atcaaacgtt tagacgatga acagctactc tatgatgtcc atttgagaca
     3001 gcgacaaaca gaaaaaatga tcgagatcta tcgagattta ttagagagta ttggtgatct
     3061 attcaacggg atgatggaca ataatctaaa tcatatcatg aaatatctag attcagctgc
     3121 attaatcatt tccgttccgg cgttgttcgc agggatttgg ggaatgaata caggaggatt
     3181 accgggtaaa agttctgctc taggctttat actcgtagtc tttggctctt tgatagcagc
     3241 agtagtttta ggttaccatt tataccggaa agattataca aattaaagaa tctatttaaa
     3301 acaggtatca aacaaagaaa tttgttttgg caatacaatt taaaaactag gaggaataca
     3361 gcaatgacag aaccaaaatt aatcgacccg cgaaacttgt accatacgga gaaatttcct
     3421 aaacaagatc aggatacacc agctttacag cagaatatga ttcctgttcc tgattgtgga
     3481 gaagaatcct atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat cactggtgga
     3541 gattcaggga tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc agatatcgct
     3601 cttcaattct tcccaggaga agaaaaagat gcagaggaag tagcaaaata cgtgaaagtt
     3661 gcaggacgta agattgtctt actacctttt gatcttcgtg acgaacaagc acctaaagaa
     3721 atcatagata aagccgtgag tgaacttggc gggctagata cgatggtact aaacgcagga
     3781 cagcaaattt cttgtcagtc tattgctgag ttaccacttg agcaagtcaa agacacattt
     3841 atgataaaca tcatcgctat gtttgcttta gtgaaagaag ctgttccgca tatcccagca
     3901 ggaggagcaa tcgtaacaac aacttctgtt caagcattca atccaagcga acatttatta
     3961 gattacgcag caacaaaagc ttcaatcgct aactttacga tcggtttagc aaaacagctt
     4021 gcacctaaag gaatccgtgt aaatggtgta gcacctggtc caatctggac accattacaa
     4081 ctagacgaag gacaaccttc tgattcgatc cctgaatttg gtcagcaaag tttactagaa
     4141 cgtgcaggtc agccaggaga attagcacca gtttatgttt tcttagcatc gaataaagca
     4201 agttatgtta cagcccaaat ttacggagta actggtggag aagcgatcaa cttataagaa
     4261 gttgatcgtt tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa aaacgatcgg
     4321 agtgatcgga acattacttt tgctatctgt gcttttgttt gcgatactat taagtgcaac
     4381 ctatccattg ccttttcagc tagagaagtt tcgattcttt tccatcacta attcatacgt
     4441 gcaacaatat gtattttggc ttgccgttgc atttgctatt ttgacaatta tcacgttact
     4501 tgttttcctg ttttatccaa aaacaatcgg aaactttcta ctgaaacatg gtgatgggga
     4561 gttgacgttg gataaaaaag caattgaagg aatggttcgt tctcacttgc atgaagatga
     4621 attcatccat tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg ccgtcaatgt
     4681 aaaaggggag ttgaaacgaa cttcagcact cattggtaaa acaggaacat tgatgaaaga
     4741 aatcgaaaat gaagtacaac acatattggg aacaaaagag ccagtgaaag tatcggtgaa
     4801 atacacaggc tttgaagaac cagctaaacg aagcaatgac cgcaaacgag tagaataggg
     4861 ggggaccaaa aatgaaagaa ctattttcga catacaagct ccctatcatc tttggggtac
     4921 ttggattgat attagccgtt ttatggatca ccgtgggctt tttcaagacc ttattgatct
     4981 tgatcatggc agcgatcggc gtcgctatcg gtttttatct gaaacaaacg aaaatcttag
     5041 atcactattt taaatcttag gaggaaacat ccatgaataa caataacaaa aataataaca
     5101 ataaaatgaa caaccaaaaa ccagtgtctg cacatccaga cccaacgaca ccaaaccaat
     5161 caaatggtgt gaacgatgaa aagaacaaaa cagcaccagt atctgcacat ccagatccag
     5221 tagtccctaa cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa aagcatgcac
     5281 ctcatcatcc aggaactcca gaatctaaag ttccagaaca acatgatgca atcgatcctt
     5341 ctaaacctgt ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa gaaaaactta
     5401 gttctgacca cacaaacgga caaggaaaac ttggttctga tcatacaaat aaccaaggaa
     5461 agcatgattc tgaacaggaa gattcaatgc gcggtgagct gaattatgaa gataaagttg
     5521 tgcaaaaaat catcggaatc gcaattgaac aagtagacgg tttattatct gcaaacggtg
     5581 gtttcttctc taatgtggca ggaaaacttg tgaatacaga taatgtaacg gctgggatcg
     5641 aaacagaagt cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg gaatatggca
     5701 aagatatcga aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa gtaaaaaata
     5761 tgacacattt agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa acaaaagaag
     5821 aatttgaaaa agatcaagaa acggtgcaag acaaagtaac tgacgcagcg aaaaaaaccg
     5881 gtgaatttgc ttcgaatcaa acagataaag taaaatcagc agctggaagc ggcgcacaaa
     5941 aagtaaaaga aaatacagaa ccacgggttc agtaataagg aagtaaaaca ttaggaggaa
     6001 cgaaacatgg gatttatttg gtcacttatc gtcggaggaa ttatcggagc aattgcagga
     6061 gcaatcacga ataaaggttc ttcgatgggg attattgcaa atgtcgtagc aggtttagtt
     6121 ggatcagcac ttggtcaagc attattaggt tcttggggac caagtattgc aggaatggca
     6181 atcgtgccat cattgattgg tgccatcatt ttagtactaa tcgtatcatt ctttgctcga
     6241 aaactgtaaa aagactttta aaaagaggtc gtgaaaaagc tgttcttcat caagtaataa
     6301 gaacagccaa acaaaaatag cttctcatat ttttcatttg gttgggcata ttatcgaaga
     6361 tgcaagcttt tgaacactgt ttatacggaa taagaggctg tgacaagact tttgtcacag
     6421 cctcttattt atgcgggcaa attcccaatt agatttttaa aagtttttcc ttaaatgtgt
     6481 ctttattgat acagagaagc ttctttaata aaacaatgcc tccctaaatt ttcttgagat
     6541 aggaaaggtg aacatacaaa ataatacgac cactttgaaa acccagatga tccacaaatg
     6601 gatcgtatga tactgatgaa gtaaaaagca atggatcaga aactcaaaga gattgaaaaa
     6661 gacaaggaag aaataatatc tcttttttac tcttgtctgt tttagccaat aatagctcgt
     6721 aaagtaatct agacaaacaa atagaagtaa acaaagtttc gtaagagcag caatcgtatc
     6781 atgttgctct gctagacgat tgacgagcag gaaaaatgtt ggtaatggaa tatagatcac
     6841 gagtaatgaa agaaaaaagt aaacaataaa tggcatacta ttcctcctaa ttgtgtgagt
     6901 tcgccctcat tttaaaataa cgatatttgt ttgtgaagct ttttcgtgtc gtaacaaatg
     6961 ttatctaatg tatttttaaa taaataatca ttattatcaa aataatgtta aaaataaaaa
     7021 agaattg
//
LOCUS       DAZKOW010000121         6843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000121 DAZKOW010000000
VERSION     DAZKOW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.123"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            526..720
                     /locus_tag="ACG912_002571"
     CDS             526..720
                     /locus_tag="ACG912_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513847.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            911..1160
                     /locus_tag="ACG912_002572"
                     /pseudo
     CDS             911..1160
                     /locus_tag="ACG912_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1404..2234)
                     /locus_tag="ACG912_002573"
     CDS             complement(1404..2234)
                     /locus_tag="ACG912_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFQ0513848.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2442..3776
                     /locus_tag="ACG912_002574"
     CDS             2442..3776
                     /locus_tag="ACG912_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFQ0513849.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            3817..4728
                     /locus_tag="ACG912_002575"
     CDS             3817..4728
                     /locus_tag="ACG912_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0513850.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4790..5578
                     /locus_tag="ACG912_002576"
     CDS             4790..5578
                     /locus_tag="ACG912_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFQ0513851.1"
                     /translation="MTVIGIILIIPLLWMVFTSLKPMEEIVRYPPTFFPEKIVWENYL
                     DTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYGFAKLDFPGKKLMFALVLSTMMI
                     PGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFNIFLMRQFYLSINNELIEAAEID
                     GANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFLGPLLYIQDQEKYTLQIGLQVFQ
                     NQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGMDLTGGSKG"
     gene            5575..6191
                     /locus_tag="ACG912_002577"
                     /pseudo
     CDS             5575..6191
                     /locus_tag="ACG912_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            6193..6711
                     /locus_tag="ACG912_002578"
     CDS             6193..6711
                     /locus_tag="ACG912_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFQ0513852.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2058 a         1186 c         1372 g         2227 t
ORIGIN      
        1 tattttataa taaagatatg atgactaatt tagctcaact attccagcgt cagctgaaga
       61 tattgcaatt cccgattcct taatcattta tctggatgag acagaaagtg aagttgcaac
      121 agatgtgcat gtagttgagg ataaaaatga tagtttaaaa attaatagtt cagatacttt
      181 actaagagta agtcctatct tttcagctgt tatttagatg caagtagcaa ctaagttagg
      241 tagtactcct gcaatggata ggtatttgtg tgttcgatat ataagtggtc gaagtatcaa
      301 ggttatgtat actggatggg agatagaaga gttaaaaatt ggaggtcacc gccacaaatg
      361 gaatttagat ttagaggtac cttagatttg gtatagttat taaacacaag atggcctggt
      421 ttgtagggaa agtcgaagag tatttttcca cttctgcgcc cctttaattt ttctagtaat
      481 gccgtgaatt tgtgattaat tggaaatatg aagaaaggag agtctatgaa aacaacacta
      541 gttctattct ataaaaagca tccttatttt acacttctga taaatatatt gttggctagt
      601 gttatcggga tttctgttga atatctaata aataaagatt tcataggttc ttgtttttat
      661 acggctttat ttcttggact tcttgaagca ttttccattt ataaaaaatc aaaaaaataa
      721 aatagattgc cgtaagagat ctttttcatt tcattttttt ctgaaaaccc gttgtttctg
      781 tcagtgaagc agcgggtttt tagatttatt gtctttagac ctaattgagc gcgccgagcg
      841 attgaaatac aaaaccactt gctatgcggg tggagcaata aagggaaata gtttagcccg
      901 tacaaagtag atgtgtaaat accacattgt attcacacca aagtatagaa gaaaaataat
      961 ttataatcaa tatcgagcca atattcagga gatcatcaag agattgtgca aatctaaaat
     1021 ataaatttgg gtatcgacac ttttgggtgg aaggatacta tgtgacaaaa gtaggacaaa
     1081 atgaagcgac catcaaaaag tacattcaag aacaagaaaa acatgatagt gcgttagata
     1141 agctgagtgt acgagagtaa gacgatcctt ttaagggcta aggagtagta cataaacctt
     1201 ttaagaggtg ggcaaaactg gcaaaggcaa tatggcttga acataaaata tgaaagccag
     1261 cgtctttagg cgctggccgg tgatttgagc ttatagctct agagaaaacc acccttttga
     1321 agggtggtta tgattaatga ctttagtcag ttttgctcac aaagcgtgtg gaacaaaaaa
     1381 cgggtgtttc tacgggtaga tatttatttc ttgaatggag gtgtcccata catttttggt
     1441 gttactggtt ctaattttgg tgcagcgcct tcgatcttag gttctttcaa ccattgatat
     1501 tcgccttcgc catccagtgc tttgccacta gcccaagaac cttcactact ttggtctccc
     1561 tctgagaagt tatagaagtc ataagcaatg tcttttcgtt ctttgtcacg cgggaaagta
     1621 cttgggacta agattgaacc ttctttttct tctaattctt tcactgcaca ttcccataga
     1681 ttttgatgat agctatctct tgcaaggaga acagacagca tatcttttac gccacgatca
     1741 tcgatcattt cgtaaatacg tgctacttga agtcttcctt gtgattcggc atttaaatta
     1801 gctctaaagt ctgctaacaa gttgccactc gcaatgatat aggcaccgct ccatggattg
     1861 cccacgctgt cagcaggacg aggtcctaag ccagctacga tcgcgtgctg tggattcatt
     1921 ccagagataa tggcaccgat tgttggatct ttttgcatcg ctgcttcttg atcttcaaca
     1981 ggcgcaccct ctaataaacg agcaaccatt gtcgccaaca tttccacgtg gccgatttct
     2041 tctgttgcaa tgtccatcag catatcttta tatttttctt ctcctcggca gctccagcct
     2101 tggaacaggt actgcatagc tacactcatc tcgccatatt ggccgccgat cagctcctgc
     2161 agatatttcg cgattaatgg attcggacga tctggttttg cttcaaactg caattctttc
     2221 tggtgtctaa acatcaaaat tcctcctaat ctatttttaa tacattctca ttctgctacg
     2281 aaagattaaa aaattcaaag aaagcgctcg cgaattatat aaagtattgg tatcattaat
     2341 aattttttta gaatcatacc ttataaattg ttattttcag aacaaaatga tatcgttata
     2401 ttaaaaccgg catcggttac attaaaattt ggaggtaaaa tatgaaaaaa ttattgttta
     2461 cagggttgct ggcaagttca gcattgtttt tattaggagg atgcggatca agtaatgcaa
     2521 gcgatgccag ttcaagtgca gcaaattctt ctgaaaaaac aagtgatgga agaaaaaata
     2581 ttgatttctg gtccttttgg ggatccggtg cacgaaaaga agctatcgaa gaaatcattg
     2641 aggatttcaa tcaatcacaa aacgagatcc atgtaaatta taagtatcag ccatggggtg
     2701 acatctggac taaatcttta tctgcaataa cagctggaaa tgcacccgat gttattgtac
     2761 aggacataaa ttccgttgcc caaagagcag aagcacagca agcaactaat ctctccaaat
     2821 atgtagaaaa agacacttct aaggattttt atccgcagct ttgggataca gtagaatatg
     2881 aaggtgatcc ttatgcgatc ccattcaata cagatacaca agtcattttt tataataaaa
     2941 agatatttaa agaagctgga atcagtgaac aagatctacc gacaacttgg gatgaattag
     3001 aagaaacagc acgtaaacta gataaaaaag aaggaaaaga atttacacgc atcggttttt
     3061 atccattatg gaatttaggg gctgatgtat gggcgctaaa cgcagataac gggactagtt
     3121 ggtttgacaa agatgaaaaa gtcaagattg atactaaaaa taaagtggaa gccttggagt
     3181 ggatccaaga ctggcaagat tattatggga aaaatacgat caataaatta gaagccgaat
     3241 ttgggtctgg tgtatctgat ccatttattt ccggattagt agccatgcgt gcccaaaata
     3301 tcaattatta ctctagtttg atggaaaatg caccggatga ttttgatttt ggcgtgatgc
     3361 agctcccaga aaaagaagaa ggttcaggcc attggtcgtg gggtggcgga ttcgtcttgg
     3421 aagtaccata cggagcaaaa gatcctgagg cctcttatga attcatcaaa tatctttctt
     3481 ctccagaagt acaagaaaaa ttcggcgaaa aaagttttga tatcatggcg agcaagacag
     3541 ccaatgagaa tttagtgaat aatgacaacc taacggataa aggaaaaatg atctatcaaa
     3601 tggcggacga aaactttaaa aatacggtga tcacaccagt tcctttagat gctccggatt
     3661 acacgacatt agtgaatgaa cagattgacc agattctttt aggaacaaaa tcaccaaaag
     3721 aaggattggc agatgcacaa aaagcagtag aaaacttagt caagcaaaat caataatcca
     3781 tttttgactg tctaggtaca gaggaggcaa gaaaatttga agcgtttatc tatccgaaag
     3841 aaaaaagaag caagatgggc ctatttattt atctcgcctt ttattatcgg gttcttggta
     3901 tttatgctag ggcctatgct tttttcagtt attggtagtt tcaccgatta taatttgaca
     3961 tccaagatga atttcattgg tctagcaaat ttcaaaagaa tgttcgttca cgatgattta
     4021 ttttggaaat cgttatacaa caccgtttat ttcgtcatct tcaatgtacc gctgacgacg
     4081 attttcagtg tgtttttagc cacagtactc aatcaaaaaa tcaaaggaat cagtgtattc
     4141 cgtacagctt tctatttacc ggcagtctta tcaggagtag cagtctacat cttatggatg
     4201 cagctgcttt ctccatctgc aggattgatc aacacatttc tgggctggtt ccatatccct
     4261 ggaccagctt ggctgtttga tccacaatgg acgaaacctg cgttgatttt gatgaaaata
     4321 tggagttctg gaggagcaat gctactttac ttagctacat tacaaaatgt tcctagatct
     4381 ttatatgaat cagcagaaat cgatggagca ggcgtttggg gaaagttcaa aagtattact
     4441 ttgccattaa tcactcctat tattttttac gatgtgatca cctctacgat cggttctttc
     4501 caaatttttc aagaagctta tgtcatgacg aaaaatggaa caggcggacc tgcaaattct
     4561 ttgctttttt ataatctgca tatgtggaac aaggcgttcg tagcttttga tatgggttat
     4621 gcgatggcta tgtcaatgat cctgtttctg atcgtgttgg ttctgacgtt tgtcaatttg
     4681 aagcttgcac ctaagtgggt ctattattct ggaggtgacg gcaaatgatg gattatttca
     4741 aaaagaccag tgtgaatcgg caaacaaaaa aatactaagt tatgtcctca tgacagtaat
     4801 aggaatcatt ttgatcattc cattgctttg gatggtgttt acttcattga aaccaatgga
     4861 agaaatcgtt cgttatcctc cgaccttttt cccagaaaaa atcgtatggg aaaactatct
     4921 ggatacgatt gccgcctttc ctttttggcg ctatgcaaga aatacattat tcatcactgt
     4981 gctagtagtg atcggtaatg tcctttcaaa ttcttttatt gcttatggat ttgcgaagct
     5041 tgattttcct gggaaaaagc tgatgtttgc tttggttctt tctacgatga tgatcccggg
     5101 gtttgtaacg atgatccctc aatatgtatt attctccaag atcggctggg taggtactta
     5161 tttgcccctg atcgttccat cgttcttcgg caatgcgttc aatatatttc tgatgcgtca
     5221 attttactta tcgatcaata atgaattgat cgaagctgct gaaatcgacg gagcgaatca
     5281 tttgtatatt tggagtcatt tgatgcttcc attgacgaaa cctgccttga ttaccattgc
     5341 gatcaattcc ttcaacgcag cttggaatga tttcttaggt cctctgttat atatccaaga
     5401 tcaagaaaaa tacacgttac agattggctt gcaagttttt cagaaccaag caacgacaca
     5461 atggaattat ctgatggcag gagcaacact tgtcttgatc ccgacgattt tgctgttctt
     5521 ttttgcacaa cgttatttca ttgaagggat ggatttgaca ggaggttcaa aaggatgatg
     5581 aatcgatcga ttggtaaaaa agccaatgat tatttcttag aaatcattga atggattcca
     5641 cgtcttatcc atttacagct actatggttc ctgtgcgtct tgcctgtttt cacattagga
     5701 actgcaagtc gaacagttct gtatatgatt tatcattaca aaaaaatgaa gagaaaaagc
     5761 tcggttcact attctggaaa aagtttcgtg agaacttctc tctttatgga aagcaagata
     5821 gtttagcaag tgtttatttt cttcttctat tgatcgatta tcagattttc cgctattggg
     5881 gtgggggaat tggctataca ttgatgtata ctacgttgtt tttgcttcta ttaagcgcgc
     5941 ttctctttag ttacaaaatc ttgcttcaac tggaacaaaa agaagcttct tggattactg
     6001 catttttctt gttttttaat gatttcaaaa gcgcgctttt ctatttggca ggcacgttga
     6061 tccttcttgt cgcattctgg tttgcaggac caatctattt tgctttatta ggtttcagtt
     6121 tcttgtttta ttttcaagtc ctgcttttta tccatcgtac gcaagaaaag gttcttaaaa
     6181 aggaggaata acatgttgcg tttgattgct aaaatcgatg atagtattat caacaagttc
     6241 gccaagcaac ggaaaacgga tagtcgaaaa atcaattttt tgaaatttat cagtaatatc
     6301 tctaatggtg gttttatttg gttgctcctt gctgctgttt tagtcttgat acctgatact
     6361 agagaatatg gtctagccat tcttatcggc agtttgatct atttttttgt tggtttgctt
     6421 tttatcaaaa aaacgacaag acggaaaaga ccttatgaaa aaaatcgtcg gcttgtcctt
     6481 tacacaagta aaccgaagac ttcttctttt ccttcaggac atacagcgct agccttttta
     6541 tcagtagctg cgttgttcga attaagtcca gttgcaggaa tattagcctt tttatttgct
     6601 tgtttgatcg cttattcgag gattttttta cttttacatt atcctactga cattttagga
     6661 ggagctattt taggaagtgt tattggcatc ctatgcgctg ccgttatctg aattaattgt
     6721 acgaataaag catgagtgcg taaatgcgca tttgtgcttt atttttttgt ttcgaggaaa
     6781 gcgtagttgt ttattcattt gtcttaataa cttaagtttt gttaaatgag aacgttataa
     6841 aat
//
LOCUS       DAZKOW010000122         6810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000122 DAZKOW010000000
VERSION     DAZKOW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.124"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            106..996
                     /locus_tag="ACG912_002579"
     CDS             106..996
                     /locus_tag="ACG912_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFQ0513853.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1069..1893)
                     /gene="nadE"
                     /locus_tag="ACG912_002580"
     CDS             complement(1069..1893)
                     /gene="nadE"
                     /locus_tag="ACG912_002580"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFQ0513854.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1905..3377)
                     /locus_tag="ACG912_002581"
     CDS             complement(1905..3377)
                     /locus_tag="ACG912_002581"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFQ0513855.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(4014..4376)
                     /locus_tag="ACG912_002582"
     CDS             complement(4014..4376)
                     /locus_tag="ACG912_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFQ0513856.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4599..5351
                     /locus_tag="ACG912_002583"
     CDS             4599..5351
                     /locus_tag="ACG912_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFQ0513857.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5491..6414)
                     /locus_tag="ACG912_002584"
     CDS             complement(5491..6414)
                     /locus_tag="ACG912_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0513858.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6429..6701)
                     /locus_tag="ACG912_002585"
     CDS             complement(6429..6701)
                     /locus_tag="ACG912_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513859.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         2095 a         1352 c         1157 g         2206 t
ORIGIN      
        1 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
       61 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
      121 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
      181 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
      241 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
      301 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
      361 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
      421 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
      481 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
      541 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
      601 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
      661 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
      721 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
      781 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
      841 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
      901 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
      961 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
     1021 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
     1081 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
     1141 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
     1201 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
     1261 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
     1321 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
     1381 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
     1441 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
     1501 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
     1561 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
     1621 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
     1681 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
     1741 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
     1801 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
     1861 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
     1921 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
     1981 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
     2041 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
     2101 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
     2161 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
     2221 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
     2281 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
     2341 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
     2401 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
     2461 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
     2521 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
     2581 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
     2641 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
     2701 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
     2761 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
     2821 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
     2881 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
     2941 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
     3001 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
     3061 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
     3121 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
     3181 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
     3241 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
     3301 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
     3361 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
     3421 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
     3481 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
     3541 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
     3601 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
     3661 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
     3721 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
     3781 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
     3841 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
     3901 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
     3961 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
     4021 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
     4081 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
     4141 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
     4201 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
     4261 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
     4321 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
     4381 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
     4441 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
     4501 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
     4561 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
     4621 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
     4681 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
     4741 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
     4801 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
     4861 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
     4921 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
     4981 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
     5041 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
     5101 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
     5161 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
     5221 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
     5281 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
     5341 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
     5401 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
     5461 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
     5521 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
     5581 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
     5641 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
     5701 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
     5761 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
     5821 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
     5881 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
     5941 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
     6001 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
     6061 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
     6121 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
     6181 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
     6241 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
     6301 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
     6361 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
     6421 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
     6481 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
     6541 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
     6601 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
     6661 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
     6721 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
     6781 gttttattcc tctttcatta ttaccaaagt
//
LOCUS       DAZKOW010000123         6644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000123 DAZKOW010000000
VERSION     DAZKOW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.125"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..859)
                     /locus_tag="ACG912_002586"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACG912_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG912_002587"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="ACG912_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFQ0513860.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="ACG912_002588"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="ACG912_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="ACG912_002589"
     CDS             complement(2143..2373)
                     /locus_tag="ACG912_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFQ0513861.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="ACG912_002590"
     CDS             complement(2603..3421)
                     /locus_tag="ACG912_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFQ0513862.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="ACG912_002591"
     CDS             3582..4271
                     /locus_tag="ACG912_002591"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFQ0513863.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="ACG912_002592"
     CDS             4285..5787
                     /locus_tag="ACG912_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFQ0513864.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="ACG912_002593"
     CDS             5800..6276
                     /locus_tag="ACG912_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFQ0513865.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="ACG912_002594"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="ACG912_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2174 a         1032 c         1203 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttattatcca aaaataataa aaaattggag cgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt catgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DAZKOW010000124         6109 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000124 DAZKOW010000000
VERSION     DAZKOW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.126"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(178..825)
                     /locus_tag="ACG912_002595"
     CDS             complement(178..825)
                     /locus_tag="ACG912_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFQ0513866.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(833..1519)
                     /locus_tag="ACG912_002596"
     CDS             complement(833..1519)
                     /locus_tag="ACG912_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513867.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1525..2598)
                     /locus_tag="ACG912_002597"
     CDS             complement(1525..2598)
                     /locus_tag="ACG912_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513868.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            2820..3494
                     /locus_tag="ACG912_002598"
     CDS             2820..3494
                     /locus_tag="ACG912_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0513869.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3499..4839
                     /locus_tag="ACG912_002599"
     CDS             3499..4839
                     /locus_tag="ACG912_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFQ0513870.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(4988..5974)
                     /locus_tag="ACG912_002600"
     CDS             complement(4988..5974)
                     /locus_tag="ACG912_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFQ0513871.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         1858 a         1152 c         1078 g         2021 t
ORIGIN      
        1 gagagactcc tttggtaggg tgacgttttg gtcgactaaa ttttaccaaa agtgagtctc
       61 tttttgagtt gaaacgaaga aaaaagtgtt agtagaaaat cgctttcgat tttccaccaa
      121 cacaaaatat tatagaacca taaaaatttc aggacaaata cacatagctg tttcttttta
      181 ttgtattcct tcagcaatcc aacgagattt atagcgaaag tcttttaaca actcttcttt
      241 tgtccaatta tttgcaatga gatccgtcaa agtgttcatt acgagggtca tggaaaagta
      301 taggaaacta actgcttctt ccgacgattg aatggagaaa tgattgattc ttaataattc
      361 cagccatttt tcgttcattg gagaatcagc cgatagttct ctacgtttgg ttgtctgctg
      421 atcatttcgc tgggttaaca agtataacct ttgccaatag atactttcgt gatctaagtc
      481 gcagcaccac tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt tttcgctaat
      541 gaattttaag atatcttgat ggactatgtg agaactgctt ttgatcgtat acaaataagc
      601 atcttccaaa tcttgaaaat atttataaaa agcaccgcgc gacatcccca tcttgtcaac
      661 gatttctgag actttgactt ggctgatgtg ctgtgagcaa aatatctcca gtaataggtc
      721 atcgattctc tgtttctttg cgttcgataa attgaaaaaa gtttcttttg gcatccacat
      781 ctctccttta tctctgatta tacgtggata gcccttcatt gtcatgaagg attcattcct
      841 tctttttctc ttcttctgtc aagtacccat cttccattcg ataaacgcga tcactccatt
      901 gaatcatacg gacatcatga gtaaccataa tagtcgcttt ttgtttctca tgagcttctt
      961 taactagtaa tttgacgact tcatatgcat gatctgtatc caaactagct gttggttcgt
     1021 ctgctaagat caagcttggc tcattatata aagcgcgagc aattgccact ctctgtcttt
     1081 ctccaccaga aaggtcttta ggataactat ttttaagtga ctggatatct aaagattgca
     1141 gcaaggaatc gatttttgtt gaatcttctt tttttgaagt aacttggtca actagttcaa
     1201 attgttcctt taccgttaaa aaaggaatca aattagagct ttggagaata aatcctattt
     1261 ctttaaagcg taaatctgct cgtttttttt ctggaagctc agttagtgag tgaccgttga
     1321 tcactatttc tccggaagtc ggtgtttgca atcctcctga aatggtaagg aaagtacttt
     1381 tacctgaacc agaaggacca atgatgctga cgaattctcc ttcctttaca taaaaatcaa
     1441 ttgctttcaa agctgagacc tttgtatgac cagaaccaaa tgttttggta acttttttca
     1501 tcgttaatat ttttttcatt tttattaacc tcctattgct gtaattgggt ctactttggt
     1561 tatcgtacgt atcgaaaaca atccgcctag cattgcgaca atgatcaaaa tcccactata
     1621 tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt
     1681 caaataagca aacaaaagag caagcagaac gcctaccacc ccaacaataa aggactgtgc
     1741 tactaaagat ttagcgatga agaaattttt gatcccttgt gctttcatta ctccaaaaat
     1801 agcagtcttc tgaagtgtca tcacgtacat gaaaataccg accacggcag caaccactaa
     1861 gaacaaaaag tatatcatcg catttaaggt catattttgg gcagaatagc caggaatact
     1921 ttcaatgaat gtcccaatag ttaataactg tagacccttc gatgcttttt ctttcgaaat
     1981 tttagctgaa tcctttatta cgatcccatt gatcggctgt tcttcttcag aagcaaacgg
     2041 ctgttgtcca tacttcaaag cagtccacgt atctagggac gcatagatga cgggagaaac
     2101 tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt aactgccaat
     2161 tttgatcgta tctccaattt tatagcctga atcagccaga ttttgagaga tgatgatttc
     2221 attctttgtt ttaaattcat gccctttcgt gagttttggt aataaaaatg cttctttcgt
     2281 tgtgccgaaa actgtaacat tttctttgtt ttttcctttg attgctccgc tgtacaatcc
     2341 gattggtgct ttctctcctc cttcaatgta tttcagatct cctcttgtta attgtgaagc
     2401 tgcaaatact tgatttgcat cctccgacaa aacaatctct tgtgcgtccc aatcatcgat
     2461 tgctttttta aactcttctg aaagtccatt cgctagtcct gacaacatga atacgacata
     2521 cgcaatcaag aacataatac cgacgatcaa gccatatcgt aatttagaat agcgaatttc
     2581 tttgatagct agaaacattt gtttttcctc cctatgatag attgatagtg acaaaatgtc
     2641 acttgttgaa tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat
     2701 caataaattg tctaagacat ctttcgataa aaccttaaca tgagtcataa aaaatttatt
     2761 taagccttat ttatgctttc catgataaac taaagtgacc aaatcaatga ggtgggaaaa
     2821 tgattaaact attagtagta gaagacgata aagtattatc caataatata aaagaaatac
     2881 tcgctgcaat cggcgagata acacaagttt atgatggtag tgaaggactg ttcgagttgg
     2941 aaagtggagt ttatgatctt gcaatcttgg atttgatgat gccggaaatg aatggatatg
     3001 aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc agtattgatt ttgactgcaa
     3061 aagatagttt ggaagataag attgaaggat ttgaacgagg tgcagatgat tatctaacaa
     3121 aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag
     3181 gtctctttga tgaaaatgtt ctgaagtata aggatatcag ttgtgattta aaaagcaatg
     3241 aagtcttgta taaagaacaa gttttgccca tacaaggaaa agagtttgaa ttgctcgttt
     3301 attttttaca aaataaggga atcattttga caaaagagca gatatttgac cggatctggg
     3361 gatttgattc tgatacgacg attacagtta ttgaagttta tatgagcaac ttacgaaaac
     3421 atctgcgaag tactggattg gataaagaaa tcaaaacctt acgtaatgtc ggttatatac
     3481 tgcaaagggt gtgatgcaat gaatggagat attacacgaa agcaacgtag tcgctttttt
     3541 gcgacaaatg tagcagcatt tgcttttatc ttttttcttt tgggattcat tactttacag
     3601 atattaaacc aatctgcata cagagaaaca gatctgtcat tagagaaaat aaattttgat
     3661 tctcgaacag tacaattaga gatacaaaga tatcagcaag gcgatccctt tttaaacccg
     3721 actcaaacgg aacaaaacag aacaaacatg gaacagccat taatggaacc atcaaatgac
     3781 cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg agatattgaa taaagcagct
     3841 attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc
     3901 atccaaaata ttgaattaaa tgaagacagt ggctcatctg ccctctcttt ccgttcgatt
     3961 acgaaaaaag cggagacgaa tcaagatggc gttgcctaca tccaaatttt agaaaataca
     4021 aatcaaatca agaattcatt agcgactttt cgcacgatta tgattttgtg tatgattgtt
     4081 ttttggattc tctctattgg tatcagttat tacttgtcta gtctaagcat gcgccctatt
     4141 attctttctt ggagaagaca aaaagaattt gttgagaatg cttctcatga attgcgcaca
     4201 ccgttaacga ttattcaaaa tagtttggaa catcttttta ccaagcctaa tcatactatt
     4261 attgaagaat ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct cactggattg
     4321 acttcggatt tgctgacaat cgctcggaat gattctgatc agcagctgct ttcaaagcaa
     4381 atgatcaata cacaagaata tatcgaacaa cttgttaaac catttcaaga gatggccatc
     4441 atagatggaa aagagtttat tctccataac gatgcaacat tagaagtacc aattgatgaa
     4501 aaaaaaatcc atcaagtttt ggttattttg ctggataatg cattaaaata tacgaatcca
     4561 gaagataaga tcattttgga ttcatctgtt aaccataaag aatggctgat tcacgtaaag
     4621 aatactggcc cttctatcag tgaagaagat caagcaagga tttttgatcg attttatcgg
     4681 gaagatcaat ctcgggcgaa agagactggc ggatacgggc ttggcttggc aattgcaaaa
     4741 caggtaatcg aacagcatca tggacagatt tctgtatcca attatcttcc acaaggagtc
     4801 gattttaaag tccaattgcc gttgaagcct aaaaaataat gcttatccct aaaaaagggc
     4861 tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat aaacggtgtt ccagaagtag
     4921 cttcttcgga aataagccga aatttcttta agctgaccac ttctgtcaca acctctttag
     4981 cttacgctta cgatttttta ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat
     5041 ccaattcttc tcggctaaca tcttctgtca ctttttcaac atcttcatgt tcttctttat
     5101 gaatcccaac ttcttcacga ataactgtat gtttgcttac atctacttgc tcttctttga
     5161 ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa gattgatctg
     5221 ttactggttt gcgttcgatg acgatttctt cacgtttgac cggtacttct accgtttcag
     5281 tatcgttgac cacatgtttg tggatgtcga cttctccagt tgtcacatca tgtgtattta
     5341 catctaattt ttcttctttt agttgaattg tatcatcatc tagatttggt ttgcgatcga
     5401 tgtcttcagt cttgcttgta cccattgtat cagtctcgcc aggtacgtcc atcggatcag
     5461 ttcctggtgt catattcaat ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt
     5521 ccattggatc tgttccagtg ccagtttcta atccagtccc cattgtgctt gctgggctaa
     5581 cagtatcttg tgtatccatt gtatcttctg ttgtttctgg ctgacgatag ccttttactg
     5641 tgatgacaaa ttttccatca gaaatatctc ctctatattg atataaagga tcattttctg
     5701 gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt atcttttatt ttttcccata
     5761 aagaacggtc ttcttcacca tccatccgat tttcatctgt ttcaacatct ataccagaca
     5821 atccgtcaaa tcctagtcta gatgctgctt catcagtagt ataaataacg atatcatctc
     5881 gttcataccc ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc ctcgtactat
     5941 agcttccgat gaccgttaaa tttttctctt ccatgtaaat tcctcctagc aaatagattt
     6001 atgattggta tattgttact tataaattta ttctatagga gacgattgtt ttctacaaat
     6061 atttagattg gaatttgatt aaaacaattt aattgaaatt tgaaaacat
//
LOCUS       DAZKOW010000125         6066 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000125 DAZKOW010000000
VERSION     DAZKOW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.127"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(254..541)
                     /locus_tag="ACG912_002601"
     CDS             complement(254..541)
                     /locus_tag="ACG912_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0513872.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="ACG912_002602"
     CDS             complement(561..785)
                     /locus_tag="ACG912_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFQ0513873.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1240..1410)
                     /locus_tag="ACG912_002603"
     CDS             complement(1240..1410)
                     /locus_tag="ACG912_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513874.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1425..1958)
                     /locus_tag="ACG912_002604"
     CDS             complement(1425..1958)
                     /locus_tag="ACG912_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0513875.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="ACG912_002605"
     CDS             complement(1979..2923)
                     /locus_tag="ACG912_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFQ0513876.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="ACG912_002606"
     CDS             3560..3646
                     /locus_tag="ACG912_002606"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0513877.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="ACG912_002607"
     CDS             complement(3767..4456)
                     /locus_tag="ACG912_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513878.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4463..4741)
                     /locus_tag="ACG912_002608"
     CDS             complement(4463..4741)
                     /locus_tag="ACG912_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513879.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(4701..5615)
                     /locus_tag="ACG912_002609"
     CDS             complement(4701..5615)
                     /locus_tag="ACG912_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFQ0513880.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="ACG912_002610"
     CDS             complement(5597..5977)
                     /locus_tag="ACG912_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFQ0513881.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1606 a         1119 c         1021 g         2320 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagc
//
LOCUS       DAZKOW010000126         5874 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000126 DAZKOW010000000
VERSION     DAZKOW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.128"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            138..1292
                     /locus_tag="ACG912_002611"
     CDS             138..1292
                     /locus_tag="ACG912_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFQ0513882.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1265..1708
                     /locus_tag="ACG912_002612"
     CDS             1265..1708
                     /locus_tag="ACG912_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513883.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2820..3788
                     /locus_tag="ACG912_002613"
     CDS             2820..3788
                     /locus_tag="ACG912_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0513884.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3798..4286
                     /locus_tag="ACG912_002614"
     CDS             3798..4286
                     /locus_tag="ACG912_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFQ0513885.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4582..4863
                     /locus_tag="ACG912_002615"
     CDS             4582..4863
                     /locus_tag="ACG912_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513886.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4865..5281
                     /locus_tag="ACG912_002616"
     CDS             4865..5281
                     /locus_tag="ACG912_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513887.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         2186 a         1014 c          960 g         1714 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatttaaca caaaggcgtg attattttga aacccaaaaa gaaaatttat ctaggtctcc
      181 tcttttcctt gatgattgga atttttagta taggaattac aaatacctat gcttcagaaa
      241 attctaattt caatatcaac tcccaaccaa ttgaagaaca agcaattaat tttgcgaaaa
      301 ctgattttga acactccata acttcaattc ttttaatgga aaataacacc gaaactgata
      361 ttaactatac tcttggttcg ccatttaaaa tgctcgatgg agaaaatacg gcaaatagtt
      421 ctattttctt tccagtattt tcagacgctg ataatacgat taaatatgtt tataccgtca
      481 aaaaaaatac aaatggagaa tattcaggaa gtatttcaca atttttagca aaagaacttc
      541 aaaatctagt agatagtaat ataacagata caaccttatt ttttcaagat aatagcattt
      601 attattccga taatggacaa attaaactac tttggaaatc acctttaagt acagaaaaaa
      661 atggtgtttc ttcccttaca gaaaaaaatg atcttatatc cgtaaatata aaaaataaaa
      721 ttaaaaattt ttctaaaaaa caaaattttc ctgcggttaa acgtgtaaaa agtgtctcct
      781 ccggagataa ctttattatg attaattggc gtatagcaga aacccaaaca agttttccgt
      841 ggtgcgctgc ttacgtagaa gctgctatct tatgtaacaa aacagatgta cgtgctactt
      901 cagctagagc cattatgcat tatacctatc ctaatttaaa caacagtcaa attgaatcaa
      961 aaagtcttaa tcaagatcaa ataataaaat atgcaaatta ctgtggtgtt tatcctaaaa
     1021 aaatagcacg aatactaaac ctttcagagg tccaaacaca actaagaaaa ggaaatgcca
     1081 tttatatggg aactgctggt tatggaaatt atagtaacag ccgtcatgca cttgcattat
     1141 ttgggtgggt caatcaaggt ggttatcaaa cttactatgt gtggaatcct tggtataact
     1201 acccaactgt aataagtgca aatgcaggac cagttacaat tcctgtacct ggaggtggtt
     1261 ttacttggga caataccatt acaaattggt aaataaactc attgctttag ttatgggtct
     1321 agtgttagtt cttttcttaa ctagttgttc aaaaaaacaa gaaatacaaa agactgatct
     1381 gattcctttt ctaggaactt ggcaaactaa agatcattca gaacgtctag taatcactaa
     1441 tttaaatact tcaaaaaata tagaacaata taccatctta gtaaaagaga acaatcataa
     1501 aattcaaatc attgataaaa atttaaatat aaatgaacaa gttttcattt acaaagcaac
     1561 aaataattta ctaacactgc gctcgaataa aaaacaatta gattacactt ttagtttgga
     1621 ttctccaaaa aagctcgtat ttattttgac aacgaatgat gggacagaag ggacagctaa
     1681 accaattgaa ttttttaaag ctgattgatg aatagttgtt tgatattaca aaacaataat
     1741 ttcaccgaag gtgaaacaag cgaaacttta caaaaagtgg ctagcatttg ctagcctttt
     1801 ttgtattgtg gagcgtgggt tttgaggggg attcaagggg gagcttgcgc ccctttgaca
     1861 agcggttatt tgcaccaaat acgtaggtat ttggctagca aatatagagc ttgccaagcc
     1921 ctttagtgtg ttttgtagaa agggacctta tagtcacttt cacattacaa acgatacgtc
     1981 ctatggacgt atcaaaaggg tgggacttat gagcaaatta aagcaaaatt tggctagcaa
     2041 tccttctaaa aaatacacct attgatcaga gtcaaaataa atcagttttc gagtgagcga
     2101 atccaaattt gcaactctga agctttgcaa atgagtgtgc cggcttccgt aaaagtctag
     2161 gtaacattag tcgatatata catagggctc taatttgcgt tttaaggcgt tttaaacgtt
     2221 ctggtatgta attataccaa agcgacttta aaacgcctta aaacgaattt tagaggttct
     2281 gtagaacgtt ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttaaaaaca
     2341 agataaggga ttttatctag acttgtctta aaacgccgta gatgagcgct cagagcttct
     2401 aaaaggactc cttacgaact gtttttaagc aaaggagcct ttaggcgact gcgcagttta
     2461 atgtgagagc gtagcgatca cacctttaaa gcgaccgtag ggagcagatt cccagtttag
     2521 gaaatttcgc gaacctgttt ggagaaaaaa agtaggaaaa ttttaggcgg gaaattttgc
     2581 ccccctcttt tataatctct tttataacct ctttttaaac ctctttaccg tcggctgtat
     2641 ccttactctc ccaaggtgtg cagaatcttt taggggtctt tttgtctgtt tttaggggtc
     2701 tttttgtctg tttttagggg tctttttgtc tgtttttagg ggtctttaat tagggggttg
     2761 acccccatta gaaagctctt tataattttg ttaagtagtt aatcttgaag gaggatttta
     2821 tggcaaatga aattgtgaaa taccaccatg aattaaatac gattccttta cgcaaattta
     2881 cgtcagtaga aatgaattta ttcttctcta taatatctag aatgcgtgat gtaggcgata
     2941 aaaaagtaca atttacattc gaacaattaa aagatttaag caattataaa gcaactgcaa
     3001 atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat ttacgttttg
     3061 gtagaagaag tgctgacggt ttgcaacgtg aacgatttgt tttatttaat caatttaaaa
     3121 tcgatggaaa agctgatatt ccttttgctg aaatacaagt ccatgagaaa gccttaccct
     3181 tacttaataa tcttgaagaa tgggttcgat attctctcca acaattcaat gaattagaaa
     3241 gcagttattc caaaaccatg tttagattat taaaacaata cagaaccaaa ggaattgcca
     3301 atttttcaaa agaagacttt catgaattac ttgatatccc taaaagttat agagaaagtg
     3361 atataaataa aaaaattata aagcctattc gccaagaact aaccgcaatc ttcaaaggtt
     3421 tgacaatcca aaagagatac ggaaaaggac ggggaaagcc tgtcattggc tatcaattta
     3481 catttaaacc tgagataaaa aaatcagacg actttaacaa acatgctcca accaaagaag
     3541 aacaaaataa agctttggaa ctagctaatc aacaggttca atctgaaaaa gaacctactt
     3601 cattacaaga aaaaatcaaa gaacaaaaac gaaaattaga acttttacaa cagttagcag
     3661 aattagaaag aaaacaaaat accccaagta agaaaattaa caaacaaata gcagataaca
     3721 attccgatat taccccagaa tttaaagaaa acttactaga taatctccaa aacttattta
     3781 aagaataagg ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg
     3841 tttcaaaaga taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa
     3901 aaggaaatat tacctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag
     3961 gtaatgaatc gggtaactta cctagtgaat tacccgattc attacctacc gattacctac
     4021 tcaaggagat tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac
     4081 ttcaaatagc tttagaaacg cagcaaaaat tactcgacca gcaacaacaa ctcaccctac
     4141 aagccaatca acaaatcaaa aaacttcacg aacaactcca gctaacctat gcagaagaat
     4201 cttcagaaaa agaacccctt tctgttactg aagaaactca aaaaggacaa aacaaggaga
     4261 aaaaatggtg gcaattctgg caataaccca acaattttta ttcataaaaa ttgttgggtt
     4321 actttctttt tttgctttta caagcatagc gacatgatct cacgtcgcta ttttttaaag
     4381 agcataagga aacgcggtat cactttctta cgaaaggtgc taggtaggct gtgcctacca
     4441 cgtttctaag aaaaaaattt gctatcactt tttcgtcatt gtggatagct tatgtatagt
     4501 gtttcggcaa aaaacagcta tacaaatact atatttgtat gatatacata aattatacac
     4561 aaatgcgaaa ggaattcttc tatggacatt ttagttcgga atattgatcc aaaagttatt
     4621 tcagaaatag atagacgttg ccaagaactc acaacaaaaa ctggcaaaaa atggagccgt
     4681 aatgcgtacc taaaagttct aatcgaaaac gattttgacc aggcacttag gcattacaaa
     4741 aaagatcaat tcgatttatt actcgaaaaa tttatcgata tacaagcaaa aaatactgaa
     4801 acattgaacg actatgttca tacaaacaac caactcattg aaacacttat cggaggaata
     4861 taacatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acattgacgt
     4921 tttagattcc atcatcgaaa aaaatccaga tatcaataac tactcggaag ccattcgttt
     4981 tctatgtatg agttatgaac ctaatactaa tacgacaact accagcaacc aagcaaaact
     5041 aaatgcgatg ggaaaagaaa tttctattat gtccgaaatc attgcagaat ttgctgattt
     5101 atatctaaaa gatactggtg ttcttatcgg tacaaattct aaagtatatc aagaagtgaa
     5161 agaaaaaatt gaaaatcaac tcaatccctc ccgacgaaat caacccaaaa atcgtcaaaa
     5221 agaaaccaat caaacattca cttctagaaa ttttgaccca acaaactttc taaaaagatg
     5281 aaaaagaaac cgaaaatttt ttctatttct cagtttcttt tttagggggc agtcttccag
     5341 caagatttgc aagttaccgg taacttgcac aaaaaaagaa aaatccgctt gtgcgaatcc
     5401 ttctttttgc attttggggc gctgccccaa tctccaaata tcggttattt aatagcgtgg
     5461 taactcaaaa aaatagaacg tactctgaaa taaaaaaaga ctgctctaac gagcagccaa
     5521 taaaaaataa tgagcgttta ttcttgaaaa agcagcgcgc ttatgaagcg ctgccaaaaa
     5581 tttaccatat tttatgaaaa ataacaagtt tctaaaaagg aatcggttaa gattcacaaa
     5641 gaatcgtaaa cagacaatag cggaaaatca agcttccgaa cgtacctttt agctagacca
     5701 atgaaaaatc acaaagtgag cccctgtatc ggttgataca agggctactt ggcatactcg
     5761 acattatgtc tactagctcg ataaataacg attatgttcc caaaacaata ttttatgaac
     5821 tataacaaaa atttgttatc gcatattgta tattttaatt ataggtgtat atca
//
LOCUS       DAZKOW010000127         5792 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000127 DAZKOW010000000
VERSION     DAZKOW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.129"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            91..756
                     /locus_tag="ACG912_002617"
     CDS             91..756
                     /locus_tag="ACG912_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFQ0513888.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(1040..2125)
                     /gene="xerS"
                     /locus_tag="ACG912_002618"
     CDS             complement(1040..2125)
                     /gene="xerS"
                     /locus_tag="ACG912_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFQ0513889.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2459..2692
                     /locus_tag="ACG912_002619"
     CDS             2459..2692
                     /locus_tag="ACG912_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFQ0513890.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            2689..3096
                     /locus_tag="ACG912_002620"
     CDS             2689..3096
                     /locus_tag="ACG912_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0513891.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(4233..4751)
                     /locus_tag="ACG912_002621"
     CDS             complement(4233..4751)
                     /locus_tag="ACG912_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFQ0513892.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            5118..5366
                     /locus_tag="ACG912_002622"
     CDS             5118..5366
                     /locus_tag="ACG912_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513893.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1948 a          931 c          915 g         1998 t
ORIGIN      
        1 attcctacct tattatcaag ggaaagtaga agaaagaaca tataaaaatc ggttatctac
       61 aatcaaaaaa acttttctta tttctttaaa atggagttaa gagaaatacg tccaattgac
      121 attcaaaagt ggcaaaataa tttattgagc aaatataaaa attcttatgt aagaaacatg
      181 tatgggattt ttcagatgtc attagatcga gcagttgttt tgggactact tgaaaaaaat
      241 cctgcaaaag ttattggaaa tgtgaagaaa aggaaaacta cagttgattt ttggacaaaa
      301 gaagaattta caaaagttat ttcaactttt tatattgatg atttgtacca acattttggt
      361 tttatttgta tgtggttact ttttatgacc gttatgcgtt tgggagaagc cactgcatta
      421 acttggaaag atattgattt tgaaaataaa aacttgcgtg tgaataaaac actctattat
      481 aagagagcca aaacctatga gtttgtagaa cccgaaacgg cttctagtat tcgtacaatc
      541 gaactagacg acaatacaat taccttttta aaagcttgga aggagcgtca agtttcaata
      601 ggaggcatgt cgtttgtact ttcttataat ggaacaccta ctcagaaata tacaatccgt
      661 catattatga gaagacatgc aacgatcgtt ggtgttcata taatccgcat acacggatta
      721 aggcatagtc atgcttcttt gcttatatca caataaaaag atgcacttcg aattaaagaa
      781 aggttaaaac atgctgatgt acaaacgact ttaggaactt atggtcattt gtatctaaat
      841 tcattgagag agataacaaa tgatcccaca gattatatcc aaactaattt ttctaaaatc
      901 aaaccgacac ctatttacga taataaaaaa taaatgataa acaaaagtag gatcacacca
      961 aaagaaacac actagaaaaa ctataataca ggcttttcta gcgtgttgtt tcttatttcc
     1021 gcctatttat acattatact cataaagcat ttaatgcatc gatcgtagcc gcattgtcta
     1081 tatgggtata taaagttgtt gtattggtac ttgaatgtcc caattgttgg gcagttaata
     1141 ccaatgaatt tgtctgttgg tagagtctag tagctaatgt atgacgcagt ttgtgagggc
     1201 tgacttgaat tctaaagcct tttgaatatt ttgctaccat ccgttcgatt gcaataccgg
     1261 atatacgctt ggcttctcct tggtaaattg ctagaaataa agctttttcg ttttctgtta
     1321 ctttatatcg ttctttccga atagctagat aattagctaa atattccatt gcgaatgctg
     1381 ctatattgac agcatctctt ttatttcctt ttctagttac taccacatta ttcttatcta
     1441 aagataggtc atctaaatta atatttataa cctcggagag tctcaatcca gatcctaaaa
     1501 tcagtgcatt gatcgccagg tctctttcta catctcgttg ataatagatc aaagctcgtt
     1561 tactcaaagt atttttatat tcattggcaa tataatctag ataattttgt gtatccttat
     1621 ctagaaataa ttttttctca atggcattgg cacgtgcagc taacgtagtt ttatctgtga
     1681 tggttgctac ttgatccata ggattattgt ctaagtatgg ttttcctaga ggattattgg
     1741 aacttttcga aagatattta aatagaacct ttaatgccgt gatatttcta ttgatagtag
     1801 aagtagatag acctaagtta ttattatctt tgagcttagt ttttgttgtc tcttttttta
     1861 cattttcttt tgtgcgacgt ttcaaatgag atttgtacaa gtttaggtca taggcagtta
     1921 actgtcttat cgaggtgata gaagtatcag gaacgataga ctcactcaga agccattcat
     1981 aaaagagctt ataatttttt agatattcta aagtggttcg aggtgattga tctttatcct
     2041 cacgatcttg aacaaaatct tttatataat cgggcatatg aagtatgatt gtatctattt
     2101 tttgatatat ttctgtattt ttcatgtcat tcatcctttc aataagtagg ggagaagagt
     2161 atctctataa ttggactata ccaattatag aatgttttgt caattggtat gctctaaaat
     2221 ttattaattt gtttcaaaaa aagcatataa acaggtatac gtttaataat ttaaaataaa
     2281 tggtatacat aaatagggaa ggaaatagaa gggagcaatc taaatttttt gaattttctt
     2341 aattaattat atagaaagaa caatctttct atagagttag acatgagagg gactatgctt
     2401 gatttaaaat caacaagcgt atatactgta tatacaaaga aaaaggaggg agaagactat
     2461 ggcaattaaa gaaagaaaat ttagggaagt ggggaactca gtagtattaa ctatttcaaa
     2521 ggagtttctt aaagaaaatg ggttaaatcc cggagatacg gtttttttag atgaagaaaa
     2581 acttcgagcg gcaattacaa aaaaacaagc cttatctgat gaaattgatt tagcaatatc
     2641 tcaagcatta actgaatatg atactacttt tagagagctt gtcgataaat gagtattcat
     2701 tacctagaag ccaaagatat tataaaaatg aatgtaatcc aaataaaaaa atactctcct
     2761 aatgaacccc ttggtgtaaa ggatgcgaat gctttagaaa tgtgtgtagg gcaacttcga
     2821 gcctcagctt ttggtgaaga ggtttatcct tcgatttatg aaaaagcagc tatcctactt
     2881 atacaattga taaagaaaca tccttttcac aatgcaaata aacgtactgc tttcttagct
     2941 acttttgtct ttttgaaaat caatggacag cttttagcta ttgatcaaaa agatgctgta
     3001 aatttagcgg tatatattgc tacgtatgat aaggatttcg atcaactaaa atatgaagtg
     3061 atcaacacta taaagacaca tacgcagccc atttaaggag ttactgtatg tgtctttata
     3121 gggttattag ctgctatagc gaaataattg aagtaaatta caaatagcta taattcaaat
     3181 ccaactatat agaaaacgta aattttctat atagttggat taagataacg actatttatt
     3241 ttatcatatt tttactatac cctatacttt tacttggtat atttatgaat aatcgcaaat
     3301 ttacccctat actactagtt tgtaagttag attaataaaa atactaaagt tatttgttct
     3361 tttattaaat acacctacaa cttataaatt gatggtattt agataacacg aaagaataaa
     3421 aataacagct gatagtaaag tttgttgacg attgctactc aatcgaaagt gagttagtgc
     3481 gtatgaactt gcttctgaaa acttatgaaa tgaaagacct tttacatctt gcagaaatcg
     3541 ttactgggat taaactaagc aataaaaact aatgttcccc tcattctttg aacttctact
     3601 atttgattta tttattctga taaatgacga tttcaccttc aggatcttca aatcccgtat
     3661 agaatttatt taagatctct tcagtatcag taataggata ggcgataggc cacataccct
     3721 ttgatggtta gaataaactg ctcaaaatct gtatcgtatg ttggctttag catattaaca
     3781 aaggctatct ccaaattata aaccataagc gttgaatcgt tcttaacaat ttcaattctt
     3841 cctaacccaa agtcagaaga tataattacc ggacttgcct tacccttcat gtgggttgtt
     3901 gagattccat atttaaaggg aattgtcggt actatcggcg gtctgaaaaa catcccaaaa
     3961 ataaaaaaat ctttcccctg ctagtctcct aagtcccttt gtagcggata tggcaccata
     4021 atctcatttt atcgaagtga gtcatggttt atctgttggc caaccaaaat tgatacaatc
     4081 taacgatgca ccctctacgg cattctaggg gagtgcactt gcattcaccc aataaaaacc
     4141 ggcagttagc aaagaactta tgtagtcgta tgttcttact gttaacatac aagaggtctc
     4201 ctttccacaa ttgcttagca gtaaacggga atttacaaag ctaatctctt ccattttctt
     4261 atctttgttc taaaagtccc aaatggagca actgtattta catgaataaa tttatatact
     4321 tcccatacag ccgttttggt tgcctcgtct gcccattttc ttttgtgtgc agtgaataga
     4381 tcttcatcac tcattgaatc aatcatcatg ttaatatcat caatattacg attgagaatt
     4441 tcctctaatt ctttcaatgg tagatgtgca taggtatttg taaaccactt atataactct
     4501 cctaactgat tccatttaaa ttgatcagaa ggcgttttaa cagataatcc atttctttca
     4561 tcattttccc attttagaat taaattcgtc caaccaactt gatatgctaa attttcagca
     4621 ggtgttctat ccacttcctc aactctttta tccttcaatt cttcaggaat agtttcaaat
     4681 tctaaaatat atttagtata agtcctattt atttcttttt ttaattcttc tttattttta
     4741 tatgttctca ttatattacc tccataaatt cctaattgtc tcctttaatt catctataat
     4801 ttcatccacg atattttaat ccgttctaca atgcatatct gatatgcatt gactgtctaa
     4861 ctcatatgtg aatgatttat aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc
     4921 agcaaggagc taaaaaatac gaatatattt cttttgtagt aaaatcggac tggtttgcat
     4981 atcattttct tcgtaaatat caaaatcatg ttaactacta tcaattacta ataaaaacta
     5041 ccactttaag cgttaagaac tgttaagttt agttaagtga ggatagttca caaaatccat
     5101 tgttaaactt cacgaaagtg gtaaaactca aaatgcttta tataggatta cagcatcgct
     5161 ctttcttcga ttgcgcgttg ggtaaaaaat attatgaagt aaaaattgac gatgacacgt
     5221 tccttacggc tcatcagatg aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt
     5281 taattctaaa aaagcgattg ccatattcac gcctcgctcg acgaactatt aaaggtcaag
     5341 atttacctga agatcatcct ttttaggaaa ttgaagcttt ttatgttgca caatgcattt
     5401 ataatactac aatgatgttt tcaccagata ttcttctagg gtgcggtgta atgaaacaga
     5461 tacacttgca aaagaaaatt caccgcgaat ttactaaaat tatgaataat tatgttaatc
     5521 ttccgaaatt tttttccaaa attaagggat caagctggtg ttatagattg tttaaggttg
     5581 gcaaaaaaat aacttctttt taaacaatta tagaaaaccg atactctgtt aagttggttg
     5641 acacggtgtc gatttttatg actttcttat tctgaaactt cactaacgga cagtacctaa
     5701 aaagtctata gtatggacgt ttacgagtac tattttatca aaaatgctgc tcatttcaac
     5761 ttatatgttt tagtgaacta cccccactta gc
//
LOCUS       DAZKOW010000128         5585 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000128 DAZKOW010000000
VERSION     DAZKOW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.132"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(488..1297)
                     /locus_tag="ACG912_002623"
     CDS             complement(488..1297)
                     /locus_tag="ACG912_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFQ0513894.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACG912_002624"
     CDS             complement(1297..2043)
                     /locus_tag="ACG912_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFQ0513895.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACG912_002625"
     CDS             complement(2061..2528)
                     /locus_tag="ACG912_002625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFQ0513896.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACG912_002626"
     CDS             complement(2528..2938)
                     /locus_tag="ACG912_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFQ0513897.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACG912_002627"
     CDS             complement(2949..3962)
                     /locus_tag="ACG912_002627"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0513898.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACG912_002628"
     CDS             4179..4913
                     /locus_tag="ACG912_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0513899.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..>5585
                     /locus_tag="ACG912_002629"
     CDS             4897..>5585
                     /locus_tag="ACG912_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0513900.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1790 a         1184 c          920 g         1691 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatc
//
LOCUS       DAZKOW010000129         5583 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000129 DAZKOW010000000
VERSION     DAZKOW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.133"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..302)
                     /locus_tag="ACG912_002630"
     CDS             complement(<1..302)
                     /locus_tag="ACG912_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFQ0513901.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYV"
     gene            395..744
                     /locus_tag="ACG912_002631"
                     /pseudo
     CDS             395..744
                     /locus_tag="ACG912_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1016..1306)
                     /locus_tag="ACG912_002632"
     CDS             complement(1016..1306)
                     /locus_tag="ACG912_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513902.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1708..1944
                     /locus_tag="ACG912_002633"
     CDS             1708..1944
                     /locus_tag="ACG912_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513903.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1951..3903
                     /locus_tag="ACG912_002634"
     CDS             1951..3903
                     /locus_tag="ACG912_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0513904.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="ACG912_002635"
     CDS             complement(3975..4472)
                     /gene="dfrG"
                     /locus_tag="ACG912_002635"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0513905.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4799..5491)
                     /locus_tag="ACG912_002636"
     CDS             complement(4799..5491)
                     /locus_tag="ACG912_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0513906.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1817 a          972 c          903 g         1891 t
ORIGIN      
        1 acatacttgc aaagtaatac tacaattcct aacataatcg tagaatttaa taatttccat
       61 agcataaagt gtttagaaaa gaacattaag aatagttcta taataattct tgaactccat
      121 gtcttataat gttctatcga agcacccaaa tagccaagtt catctaatct acttgcataa
      181 actacgtcat ccgctctcat agtcggcata tacatattaa agagtaaaaa gataccccaa
      241 atccctatta gtaacaagat cagttctttg ttattcgtta tttttctatt atgtttgttc
      301 atttttatac tccttcttat atttcctaat agagtataac atagctttaa gcattactta
      361 aagataactt aaaagttatc ttttaaatac ttaattgtta aataccttat ttgatgcaaa
      421 agaaatctac gatagtcaac aaaaaaattt tcattatatc cagaaaaatt tttcttagtt
      481 aaggacctat ggattgcagt aatgcagaat tcgtcgaata aactgccaaa gacatacaat
      541 catattgctt ttaaaattga cgaacaagat atagattcgt ttgtctcaaa aatccaaatg
      601 ttaggtttaa ctgtcgaacc aggacgttct cgtgtgaaag gcgaggcttc ttcaatttac
      661 ttttacgact atgataatca cctcttcgag ttacataccg gtactctcca ggaacgttta
      721 aaatcctaca attccactac ctgagactag taatgactag tctcttttta tggcctctga
      781 tccccccatt gagcgaatac taatagggct tcgaattgat ctgttcaatc tttaagcaat
      841 atccctcaaa tgagtttgga aagtttagtg attcatcaca aatacttaaa tacatgtagt
      901 tatatataga ttgaggttct tgtgagcgta tgctcgtgtg ggttcaaatc ccatcgtctg
      961 catccatctt tccaaaacaa gcggctagga catttttgtc tcagccgctt gttttctagt
     1021 ttattctttc atcttgtcgg accttttttc ttgggtatag acttttctca tattctacca
     1081 caattttggg tttccctaac acatgatagg atctcacaac aaattgcttt ctattattat
     1141 aataacctgt aacgactaac ttcatccctt cgtctacttc agctaggaag ttcaaactat
     1201 gggaagcaat caaacaattg accccattta aactgaaacg gatcaacggt ctggcactaa
     1261 cttgcaaagt acggatctta tctactgttc catatttttt ctccataacc ttcacctcaa
     1321 taccattata cgaacataca ttccctttgt aaagaagaaa ctctgtttta tctttttgac
     1381 tttttgtcta ttacctttct taaaatcata ttggcacttc ctgcgaagat gaaggacatg
     1441 tactaaaaaa aagaggcagc agtatagatt taagtagttc agttttggag tacaaaaagt
     1501 gaaaatgtaa acctaaatga gtatcaaatt gcaactgaat ttgatactcg ttttttattg
     1561 ttaaatcaac gtttgttgtt aatttacacc acaaaatgta agttaaaatg tatataaaaa
     1621 tgcgaacgtg aaataattat tgtaggaatg caaattggtg tgttaaaatg gttacaattt
     1681 actaagcaaa aaggggtgca ttttcctatg caatttcttg agccaaaaaa taagaacgct
     1741 aagtctgtgg attgggaaat atcagagcaa gtaagggtga tagttaagca atacgctgaa
     1801 tatgcagagc gtactgaaag cgaagcagtg gatgaattct tattgaacat tttagacgat
     1861 aagaagttta ttgaatggat tgccaacaag cgaagtaata agcggattgt tgaaaaaatg
     1921 ggtatcaaag atagagtggg ttgatagaca atgaccaagc ttaaaagatt agccacgaag
     1981 gaagatgaaa ttgtagatgt taaaattccg atttcaaacg aagaattaaa agatagggct
     2041 aaacaatatg atttattaac tccaaaacga tttgcaacaa ggtataacaa aatgttgttc
     2101 ttgccaacgt cttttaaatg gaacggttct gaatatccca ttcaatataa ttactgcatc
     2161 aatccgtttt gttgtaattt tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa
     2221 ccaagtcgat ataaaatgac tggtagttcc aaagataaag gtcataaagg tatgtactgc
     2281 aatgacaatc ctattggcag aggtgtatcg caaaactgta cagtaacccc tttatctaat
     2341 tggtctgttg ttgaagaaat taaacgcctt atagaaatca atagcatcca agatgttgag
     2401 ccagattatc aatttcataa agaaggctgc tcggaagaag aatcaacccc attcaatgag
     2461 ccgaagcaat tttacaaaag ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag
     2521 gcgtgtaaga agttcactaa tgttctacca aaaagagaag aaaccactac ttatcaccaa
     2581 caaaagaaca ccattcttcc aatgttcgct aagatggttg ttggaagggt ttcagtcagt
     2641 cgtacttgtg atattttagg aattggagtt ggaacctact atcataagct ggagtggctg
     2701 tataggcgtt gtttggagtt tttagaacga tatgagacac aaccccttca aacaaagaaa
     2761 tttaacgaga tgtggcttaa tacggacaag atgcactact acctaaataa cgtccgtaag
     2821 aaaggacaag gttcaaagaa atatacgggg tttgaagacc ttaatatgca aacgtatata
     2881 gttgtatctg ctgaggtctt atcaagatat gtgttccgtt ctgatgttgc ctatgattgg
     2941 aatatttcaa tggatgaatt aaatgaagat acaagaaaat tcaaagaaga ccatctcaat
     3001 acttttagtc gtaaaaatga taggttagat tggtcttatt atccacaaga accatccgct
     3061 aacgatagcg aaaataggaa tgcttatctt cacgaattag ggaaaattac taataggagt
     3121 agatttgtcg atggtttaaa tgttgatgct ccttatacta caactgctca ctattggtta
     3181 attaaacaaa tggttaatgc cgacgaatgg cgaatgatta gtgatgatga cttctctatt
     3241 agaaacgcct tttatagagt attcacgaaa gaattaaggt tgagcgatgc acatcatttt
     3301 atttgccaag ttaacaagac gaaaagcaga aaacaatgct taaaagaatt cggacaagca
     3361 aaagcagaac tgctcgattg gggagatatc agaggattca aaacaaagtt tctacggact
     3421 ttggcatctc attatctaac tgaattacta acaagtcatc aattccacga agaggctatt
     3481 agtaaagatg gagaaagata tcgcaaatat gccgataacc ctatcaaaca tccattagcc
     3541 actaaggata aaggatttta ttcagttgat tgtagaacgg atttatctgc tctggaacca
     3601 aacgaaatcg caaagatgct attaaatgtt aatgaccatt cgaccaatag ctttattcaa
     3661 cagataagac gttatatatc atctcttgaa agaccattaa caacagctcg tggtgataag
     3721 aagagctata tttacgctaa cttcaatccg aagtatgctc aatttgcgat tacaattcta
     3781 agaacttact acaacttttg tcgccctttt aaatcagcgg ataaaaaggt tttgacacct
     3841 gctcaaagat tagggattac agataaacaa tttgattgga aagatataat ttatttcaaa
     3901 tgacaaaaag agcttggagt aatcccaagc tcttgtgctt tatctttact gttcttcttc
     3961 aattaacgca cccgttaact caataagttt tttctttcat atacatgaaa atagtagtta
     4021 tacggatttt tatcattctt tatccctttt tgggcaaata cctcattcca ttcctcataa
     4081 ttcacttctg gaaaaaaagt atctccttcg aattcatgat gtatttttgt gatgtacatt
     4141 ttctcaacat aagggaaaaa caaattataa atctgttctc ctccgaaaat aaaaatttct
     4201 tcttcgtttt tacataactc aaaaacatct tctattgaat gaacaatttc acaaccatta
     4261 aaggtaaacc ccttatctct cgtcagaata atatttcttc tgtcaggtaa ggctcttccg
     4321 attgattcaa ggttcttcct acctaatatt atcggatgtc cctttgtagt atttttaaca
     4381 tattcccagt ccttgggaat cctccaagga atgtcattct ctttgccaat cactctattc
     4441 ttatccatcg cagcaatcaa agaaactttc atttataaca cagccttttt aactaattta
     4501 ccatctaatt tcttatttct atcaaaaaac cttttctcat tatcctgccc gttagttgaa
     4561 taagcaattt tttctataag ttatattatc ataataccac aaaatcacaa attttcattc
     4621 aattatcatt ttcaattgca ttttataaat cattttgtat gtaatttggt atgtaagtta
     4681 aaatgaaaaa agtagtccat tttgaagtac attttgtcat tttgtactcc aaaactgaac
     4741 tacttagtat agattactgt tgcttcttta ctaatttagg tttacttgtt aagaaagatt
     4801 atttacgcaa atgttcttca acaatttgct gagatttatc agctgtatct tccacttcat
     4861 cttttgcttt ttctaacttt tgaatatatt gtttcaagct tgagttttca gcagttgctt
     4921 cgtctaattt cttttgtgta tctgttaact gagtttctac atttgttttt tcttgtgtca
     4981 gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa agttttgctt
     5041 gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca tcattttttt
     5101 ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt tgtgcatcct
     5161 tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt ttttggtttt
     5221 ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct tgcaatgtct
     5281 gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta tctgtcaatg
     5341 tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct ccaccccaca
     5401 aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga aatgcaacga
     5461 caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc aatactttta
     5521 catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaatta catatgtaat
     5581 ttt
//
LOCUS       DAZKOW010000130         5573 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000130 DAZKOW010000000
VERSION     DAZKOW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.134"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            129..2189
                     /locus_tag="ACG912_002637"
     CDS             129..2189
                     /locus_tag="ACG912_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFQ0513907.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2272..2841
                     /locus_tag="ACG912_002638"
     CDS             2272..2841
                     /locus_tag="ACG912_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFQ0513908.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3016..3822)
                     /locus_tag="ACG912_002639"
     CDS             complement(3016..3822)
                     /locus_tag="ACG912_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513909.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3960..5474
                     /locus_tag="ACG912_002640"
     CDS             3960..5474
                     /locus_tag="ACG912_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFQ0513910.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         1986 a          964 c         1127 g         1496 t
ORIGIN      
        1 aacaataaaa aatcaaaaaa aatactctcc taatagtcca tcttcttttg attttattta
       61 tataatgaaa tcatcttatg aaaattacta cttcatgttt agacaagagt agggaggact
      121 aagaataaat gtgttcaaaa agacgttcaa aacaactaaa taaacacgct tgtcgtttag
      181 ataaggaacg gtcgtgttct gaaaaacact acgttaagtt tatgatgcgc acgatgctga
      241 tcagtttatc tgtaggtgtc tatatgcatg tacccgttca ggcaacgaca caaaccatca
      301 aggtcgtacg tacctctgtt tctgagacca ctgattcccc tatcactatt actcatactt
      361 tcactgatgc aaatggaaaa cagctacttc ctgcattgac ttcctataat aaagtcagtt
      421 atcctccaac gataaaccac tactggttaa ttcctaacaa aacattatgg acatcggaaa
      481 ccggaagcag tgtgccgctt tctatctttt ccgatatgac tgatcctaca aaaggtgatc
      541 agttaaaaca gatcactgat attttgaatc atgctttgac agatataaag gacggtggaa
      601 gcgcacaagt ttcttatgta tatgaaaaag attattcaca gtttgaaaga aagaaaatcg
      661 atgttctggc agggacaaaa gtttcgatat ctgatctggt agaaaggctg aagaacgcag
      721 atggaacaca aggagatatg tccaaagtaa tttcaaacaa tgggaatatc gtggatacga
      781 ctaatacagg aacatctact atttccttgt cctattttga tccaattgct ttaaaaacaa
      841 tgactgcatc tgctgtgatc catgttcgtg ctgatcaaac agctatcaaa ggaaaagatc
      901 ttcacttaag aacaggagag agttacgccc ctaaagatct tgttgaatat gtaacaaaaa
      961 gcgatggaac ttcaggaaat aaagaagaaa tcaaaatgaa cgggacagta gatactacaa
     1021 ctccgggaga atatcccgtt gtattaactt atatagactc gaaaacatca aatgtggcag
     1081 aaacaacagc tgtagtttat gtagaagcat ctgctgcttc atcgactgtc atcactcatt
     1141 ttatagatag tcaaacaaat aaagaacttc aagcagaaac acatatgagt agtgtaaatc
     1201 gtattcctga tttgcgccta cctgctgaat tttctgatcg agtgccaaat atagatttat
     1261 cgacttatga acaaaatggg aaagtcactt ctatttctga ttggatgcaa gcaaacggat
     1321 tagacagaaa aagtcactgg tcggagatcc ttgaagtact tcactcagaa ctgatgaata
     1381 ataggaagca atcaggaggg gaaattacct ttacttatgt ttatatacca gacaatcgtt
     1441 cattagaagg tcatgatctt attgttcatc caaacgaaaa aataaacaaa gaagagttga
     1501 tccaatcagc aacaaattcc ttaggtaaac ccgtaccgtt aaaggatgta tcggttacaa
     1561 tcaatgaagt accattggag gatggttatt tgaccaaaca aacaggtgat tttacgatta
     1621 tgtatagtta tcatgacccg tattcactcc aagaactcca tgctcagtca ctattacatg
     1681 ttttaccatt aaacaaacag atagatcaaa aagaacaaaa aagacaagaa gaaagcttgt
     1741 cagaaaaaaa acaacaaatg gctcatgcag tttctccaac agggaaagta aaacaaacaa
     1801 gaagcctaga gttagcaaga atatctagaa aaatctcaga atctaataaa acgataaggc
     1861 aagaagctca tttggcccca caagtttctg gattggaaaa tgaaatttta gagcctaaaa
     1921 taaaacaaca atcagaaaaa gaatttgttg ctaaacagtc ggaagatcag aaggaatcag
     1981 atgaccagaa ggaaacgaag aaaaaacgct tgtcaactat tgattccacc gatgagcatc
     2041 tgcagccgca agtaccactt cctagtccgg gaacacccgc acctggtgga ggtattcctt
     2101 cttctgcttt aggagatttc atgcgaggta tggcaggaac atgggtatac gctaatcgaa
     2161 atgaatttga cgcagatgac tggttctgat ttatatctct atctgttata gtcaaataag
     2221 tagctgagtc atatgttatt tggcataatg gagacgagga gggatagaaa aatgttagat
     2281 aaaacgttac cttacgcaga aatctggatg acaagaccac ttcaacaaaa tgttccaatc
     2341 acgcctttag cggaaggttt tgaatttcga ttttatcaaa aagaagatga atctgcttgg
     2401 gcggagattg agacttccgt tggggaattt caaacgacag atgaagcact tgagtatttt
     2461 caacaaaact ttgctcctta tcctgaagaa cttgaaagaa ggatgttttt tgtagagaca
     2521 aaaaaaggag aaaaagtagc aacctgtacc gcttggcgaa aaaaatcaag agagcaagtg
     2581 tatccggttt ttcattggtt agcagtgaaa ccagattacc aaggaaaagg gctcgcaaaa
     2641 gcattgactg ccagagtctt gcaagaattt cctaccctcc atacggacgg accaatctat
     2701 cttcacacac agacatggag ccatcaggcg attgctcttt ataaaaaaat gggttttact
     2761 tttattgctg aaaatctaga cggcacacct aatccagatt atgaaaaagt tatgcaggtt
     2821 ttaagtaaat tagataaata acgaaatggt gaaataagtc ctcagtattg ggataaaaaa
     2881 cgaaatagtc attcctcata tcttactttt ttgagttaat actatcggat aactaatggc
     2941 atgccataca ttcaaaaaag aaggggcagt gacaaaagta ttgtcactgc cccttctctt
     3001 gttttttagc atgttttatc gtttgatttt tttaggaata aaattcttga tgaacgtaaa
     3061 tttttggtaa tcgtttcgac tatagaggta accaaataca ctgaaaacaa ctgctccaat
     3121 aaaattaaca aataaatcct tcatagtatc catgattccc acgtctaagt aaccattttg
     3181 gatgattgtt ttatctgttt taccatctgc attttgactt tcaatgattg tccgagtgat
     3241 atctggaaca tgatagacag tgtttccgtc tttacttaac tccacactat tgatttgctg
     3301 gatgatcgta tctttttgca tatcgaattg cagtaattta tctgctgaaa attccacaaa
     3361 ctcccacata acaccaactg tcatcgaaaa acagaaggaa acaactgcta gtaataaagg
     3421 agtcaagttc atggtttttg tatttttatt gagcaaataa acaagggaaa agccgacacc
     3481 cccagctaaa aatccgctga atgcatgtag aaaagtatcc cacattgaaa aatagccata
     3541 aaaatcactt aactctccta aaactgttga actaaaaata aaagataaca aaatgatttc
     3601 tagcatattt ggaatctgta tctttaatat cttttccaga aaataaggga tttcaaataa
     3661 aattaatgtc agtaataaaa gcattgtatt tccccagtgt tggttccaga cctgatccac
     3721 tagcataata acgacgatga tacgtaataa aatatagatt ccgtttgtga ttctacctgc
     3781 agtttttgta ctgttcgtaa aaaaggtttt caaatgattc ataaaataac tccttcatgt
     3841 atatccttaa ttatactaag aagcgtgtgt ttaccaaagc tatatgactt aaagaaaaac
     3901 agagttgata ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg gggatacaaa
     3961 tgaaaaaatt agttgctggt ttattaacag ccgttgcact tcttgctatg agcgcatgcg
     4021 gcgcagatca gaaaaataca gcgcagacaa gttctagtac gaaggaatct gccgaagtat
     4081 catcaggcgc ttctgaacaa gtatatacag atccaaaaga aatgaaagaa tcttatgatg
     4141 tcgtgattat tggagctggt ggtgcaggga tggcagcagc tatcgaagca aaagatgctg
     4201 ggttgaatcc agtaattttg gaaaagatgc ctgttgccgg aggaaacaca tctaaatcat
     4261 caagcggtat gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc aaagactcga
     4321 atgatgcctt ctatgaagaa acgctaaaag gtggtggagg aacgaatgat aaagaaatgc
     4381 ttcgatactt tgtcgatcac tctgctgatg cggtcgattg gctagatcaa aacggtatca
     4441 aactggacaa cttaacgatc actggaggga tgagtgaaaa gaggacacat cgtccgtctg
     4501 atggctcggc tattggtgga tacttagtgg aggggttatt gaataacgtc catgagcggg
     4561 aaatccctat ttttgtcaac gcagatgtga cagacattaa gaaaaatggg gaaacggtca
     4621 atgaagtgac tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga aaagcaatcg
     4681 tagttaccac aggcggtttt ggtgccagca aagaatttat tgaaaaatat cgtcccgatt
     4741 tagctgatta tattacgaca aatcaagaag gatcaactgg agatggtatc accatgattg
     4801 aaaaattagg tggacaaacc gttgatatgg ataaaataca aattcatcca actgtgcagc
     4861 aggatgaggg ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc ttggtcgatc
     4921 aagaggggaa aagatttgtc aatgaaatgg atacaaggga taaagtatct gcagcaatta
     4981 cagcattgcc tgaaaaatca gcttatttga tttttgacca aggcgtgcgt gatcgtgcca
     5041 cagctattga attttatgac caaaaaggat atgtcacaac tggaaagaca atggaagaat
     5101 tagcagaaaa aatcgattta ccaaaagaaa cactagcaaa aacagttgat ggatggaata
     5161 cggatgttgc ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg gaacatgact
     5221 tatcgtctcc gaattattat gcgatcaaaa tcgcaccagg aatccattac acgatgggcg
     5281 gtgtgaaaat caatacgaac actgaagtat tagataaaga aaaccagcca attagggggt
     5341 tatatgctgc tggtgaagtc acaggtggct tacatggaaa caaccggatc ggtggaaatt
     5401 cagttgcgga gatcattatt tttgggcgac aagcgggcat acaagcagct cgatttgctt
     5461 cggaaaatga atgaaataga acgtaaataa aaagaggttg tgaaatcagc gttttgagct
     5521 aataccgagg gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa aag
//
LOCUS       DAZKOW010000131         5481 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000131 DAZKOW010000000
VERSION     DAZKOW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.135"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG912_002641"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="ACG912_002641"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="ACG912_002642"
     CDS             complement(580..1353)
                     /locus_tag="ACG912_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0513911.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="ACG912_002643"
     CDS             1497..1847
                     /locus_tag="ACG912_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFQ0513912.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="ACG912_002644"
     CDS             1828..2544
                     /locus_tag="ACG912_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFQ0513913.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="ACG912_002645"
     CDS             2544..3992
                     /locus_tag="ACG912_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0513914.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="ACG912_002646"
     CDS             complement(4062..4571)
                     /locus_tag="ACG912_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513915.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="ACG912_002647"
     CDS             complement(4561..5286)
                     /locus_tag="ACG912_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513916.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         1864 a          955 c         1095 g         1567 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt t
//
LOCUS       DAZKOW010000132         5453 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000132 DAZKOW010000000
VERSION     DAZKOW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.136"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..847
                     /gene="purR"
                     /locus_tag="ACG912_002648"
     CDS             29..847
                     /gene="purR"
                     /locus_tag="ACG912_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFQ0513917.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1350..2723
                     /gene="glmU"
                     /locus_tag="ACG912_002649"
     CDS             1350..2723
                     /gene="glmU"
                     /locus_tag="ACG912_002649"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFQ0513918.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2817..3788
                     /locus_tag="ACG912_002650"
     CDS             2817..3788
                     /locus_tag="ACG912_002650"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFQ0513919.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3794..3896
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3962..4531
                     /gene="thiT"
                     /locus_tag="ACG912_002651"
     CDS             3962..4531
                     /gene="thiT"
                     /locus_tag="ACG912_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFQ0513920.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4612..5385)
                     /locus_tag="ACG912_002652"
     CDS             complement(4612..5385)
                     /locus_tag="ACG912_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513921.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
BASE COUNT         1782 a          967 c         1158 g         1546 t
ORIGIN      
        1 taaaatttct taaaaggagt tggaataggt gaaagtacgt cgcagtgaac gtctagttga
       61 catgacacag tatttattga atcacccgca tgaattgatt tccttaacaa gctttgccga
      121 acgttatgaa tctgctaaat cttctatcag tgaagatttg ggaatcatca agaaaacatt
      181 taaagaacga gggtatggaa ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt
      241 catccctgaa atttcatatg aagaggcgaa aaaatatatt gaatctctag cagaacgtct
      301 atcagaacaa gaccgtctgc ttccaggagg atatgtttat ctctctgatt tgttaggaga
      361 acctgaactg ctgcgtcaag ttggaagaat cattgcttct aaataccttg gtgaaaaaat
      421 cgatgctgta atgactgtag ccacaaaagg cgtacctatt gcccaagctg tgtcctatta
      481 tctgaatgcg ccattcgtta ttgtacgccg ggactcaaaa ataacagaag gatcaacagt
      541 aagtgtcaac tatgtgtctg gatcttctga aagaattgaa aagatggaac tgtctaagag
      601 aagtttgaaa cgtggttcac gtgtccttgt agtggatgat ttcatgaaag gcggcggaac
      661 agtcaacgga atgaagagct tgattgaaga attcgaatca gaacttgttg gcatcactgt
      721 atttgccgaa tcaaaattca acggtcatcg cgccatcgat gattatacgt cactgcttta
      781 tgtcaaagat gtagatactc aaacgaaaaa tattacggtt gtgccaggaa attatttttc
      841 agaataagag gttgtgaaaa agccgcttag tttcgagtag taagaaaaat tttgtgaaaa
      901 tggctttcca cattttttca taaatttggc ttaataccga gaaactggct tttgaacacc
      961 gtttatctag aggttgtgaa atcagcgtcc tgtcctgagt attagctcaa taaacggaaa
     1021 aattgctcct catattttgc cgtttattga gctaataccg aaggtcagct gattgaaaaa
     1081 cgtttaatga agaggttgta agcctaccgt ttaactctaa gtcacaagaa acgatttcaa
     1141 aaaacagctt ttcatgtttt atgagtagtg tgacttgtga ccgaggagtt ggcaggagag
     1201 caccggttac caaatagata ggcccgttat ggtctatcta tttttttatc cagaaaacgc
     1261 tgggataaaa gaaaatcact tgttttgttg aaaaaatggg tatactggga taaactgaaa
     1321 tcagcaaact atatttagga gtgacgcagt tggacgcacg ttatgcaatt attttggcgg
     1381 cagggaaagg tacccgcatg aagtcaaaat tatataaagt tttacatccc gtttctggta
     1441 aacctatggt agaacatatc attaatcggg tcagtgaaac gaaaccagat gaagtgatta
     1501 cgatcgttgg acacggagca gaacaagtta aagcacaact aggagaacgc agtaaatatg
     1561 cgttacaagc agaacaattg ggaacaggac atgctgtttt gcaagctgct tctttcttgg
     1621 aaggaaaaaa aggaacaacg ttagttatca gtggcgatac accattattg actactgaaa
     1681 cgctgaacaa tctttttgaa tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg
     1741 cacaagcaga aaacccaaca ggatatggac gaatcatccg tgaccatatt gggatcgtag
     1801 aaaaaatcgt ggaacaaaaa gatgcaacac cagaagaagc tcttgtacaa gaaatcaata
     1861 caggaactta ctgttttgac aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa
     1921 acaatgctca aggtgaatac tatctaacag atatcatcga aatcttaaaa gaagaaggcc
     1981 atacagttgc tgcttatcaa acggatgatt ttgaagaatc aatgggagtc aatgatcgga
     2041 tcgcgttagc taaagcaaat gagatcatga gaaagcgaat taaccagatg catatggtca
     2101 atggcgtaag ctttgtcgat tcagcaacga catacatcga tgcaggcgta gaaatcggtc
     2161 cagacacatt gatcgaagca ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt
     2221 gtgtcatcgg gtcacattca aaaatcgtag acagtaggat tgaggatcac gtcgtcattg
     2281 aaaactcagt cattgaaagc agccatgtca agaaacatgc agatgtggga ccttatgcac
     2341 atttacgacc aaaagctgaa attggcgaaa acgtccatat cggaaacttt gtggaagtga
     2401 aaaatgctca aatcggcaaa ggaactaaag tcggtcactt gacttatgtt ggtgatgcta
     2461 cgctgggaga agaaatcaat gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga
     2521 acaaacatca tacgactgtc ggcgaccaca gctttattgg ttctagcacg aatatcatcg
     2581 gacctgtcga ggtagctaaa aacagttcga ttgctgccgg gtcaacaatt acagacaata
     2641 ttccagaata tgcgctggca attgcgagag caagacaagt caataaagaa ggatatgcaa
     2701 aaaaactccc ttatctaaac taagactttt gcaaaatcac ttgatttatt taaaaaatat
     2761 gactactatt tattaggtag aattctagag ttgtgaaaaa gaaagtggag gtccccatgt
     2821 caaaacatta ctttgaccca agattaaaaa tttttgcgtt aaattccaat cgcccattag
     2881 cagagaaaat tgcagcagca gtcggcgtcg agttaggaaa atcatctgtt actcaattta
     2941 gtgatggaga aattcaagta aacattgaag aaagtattcg tggatcgcat gtctatgtta
     3001 ttcaatcaac gagcagccca gtcaatgata acttgatgga gttgctgatc atgattgacg
     3061 cactgaaacg agcaagtgca aaaacaatca acgtagtcat gccttattat ggctatgcgc
     3121 gtcaagaccg taaagcacgt gcacgggaac caatcacagc gaaattagta gcaaacatga
     3181 ttcaaaaagc tggtgcaaca agaatgctga ctttggacct tcatgcagtc caaatccaag
     3241 gtttctttga tattccagtc gatcatttga tgggtgcgcc acttattgcc aattatttct
     3301 tagaaaaagg aatcaaaggc gatgatgttg tcgttgtttc accagaccat ggtggtgtca
     3361 cacgggcacg taaattagcg gaatttttga aatctccgat tgcgatcata gacaaacgcc
     3421 gtccaaaagc aaatgttgca gaagtaatga atatcattgg acaagtcaaa ggcaaaactt
     3481 gtgtactgat cgatgatatg atcgatacag caggtacgat cacattggct gccaatgctt
     3541 tgaaagaagc tggtgcaact agtgtgtatg cttcatgtac acatccagta ttatccggcc
     3601 ctgctttaca aagaatagaa gattcagcta tcgagcgtct agttgttaca gactcgatct
     3661 atcttccaga agatcgtaaa atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg
     3721 gagatgcaat caaacgtatc catgaaaaca aaccagttag tccattgttt gaaacaaaaa
     3781 ataaataata aataaactca caaaggggag tccaattggg ctgagattga atctatttct
     3841 aaacccttcg tacctgtatc ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg
     3901 actattttcg aaagctgctt cctcctttgt gactttttta ttcaaaggag gaagtttttt
     3961 tatgggaaaa aatagagttt gggtagaagg aacgattgtt gcagcgttag cgatggtgtt
     4021 gtcgttgatt ccgatccaga tcggcagcag tttttctatt tcattagggc agattccatt
     4081 gactcttttt gcgttaagaa gagggacaaa ggcagggatg ctggcaggtt tcatttgggg
     4141 catcctacat tttccgttgg gacaagttta ttatttaagt gtcgttcaag tgttgattga
     4201 gtacccgatt gcctatactt ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa
     4261 aggctatgta gacgataata cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat
     4321 tggcgcactt tccagatatt tttggcattt tattgcagga gtggttttct ggggagcgta
     4381 tgcactatgg gggatgaatc cttggctctt ttctttcgta atgaatggtg ccagcgggtt
     4441 gagtacagct attgtaacga tcatcgtttt attagggatc caacaaacga ctccttcgat
     4501 ttttgtgcca gataaacgac aggagctgta aataaaaaag aagcaagaaa cttgagacag
     4561 aatcatgtcc tagtttcttg tttccttttt attttttgat aagaaataga tctacttcaa
     4621 tggcagataa gaaagatcgg catcttctac caattgccag tgtcgatcgg tgacagagta
     4681 gattaaagta gcgatgattt cctcttctcg ataccgggtg ccttttaatt tccaaatata
     4741 atcttcaatc acatagattg ggatacgata tttaccatcg atcaaaccaa cttgatcaaa
     4801 agaaaaaatg atcccttcct gcatcaaatt acgcatttct ttcaataatt ccacactgat
     4861 tttttcaaca ggtgtttctt ttttgaaaaa agattttttt acattgatct tatctgacaa
     4921 gatcgaactt aatttactag caaaaatcct ataaaattca tctaaatagc gataataaat
     4981 ccgattcagc tgatcattaa gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa
     5041 cggcgaatga agatgtgttt tcatatgacc aaaatataat aattctgaaa tttctaaagg
     5101 tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc
     5161 acgcctgtta cgagaagagg aaaagaaacg ctgaatattt tctgtaccac ggattacttt
     5221 cagccctgta tgggattcat attctccagc agaagcagat ggatcaagca agagcaagtt
     5281 ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac
     5341 agcattactt gtggtatcaa tatgtacata aactgactca gtcatcatgt tcctcctctt
     5401 atttcctctt aatctctttt attttacaac atttgcatta aaaaagcttg ttt
//
LOCUS       DAZKOW010000133         5407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000133 DAZKOW010000000
VERSION     DAZKOW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.137"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..407)
                     /gene="prgO"
                     /locus_tag="ACG912_002653"
     CDS             complement(132..407)
                     /gene="prgO"
                     /locus_tag="ACG912_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFQ0513922.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(379..1332)
                     /locus_tag="ACG912_002654"
     CDS             complement(379..1332)
                     /locus_tag="ACG912_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0513923.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1944..3437
                     /locus_tag="ACG912_002655"
     CDS             1944..3437
                     /locus_tag="ACG912_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0513924.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3572..3868
                     /locus_tag="ACG912_002656"
     CDS             3572..3868
                     /locus_tag="ACG912_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFQ0513925.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3969..4820
                     /locus_tag="ACG912_002657"
     CDS             3969..4820
                     /locus_tag="ACG912_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513926.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4841..5053
                     /locus_tag="ACG912_002658"
     CDS             4841..5053
                     /locus_tag="ACG912_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513927.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         1857 a          836 c         1011 g         1703 t
ORIGIN      
        1 gaaacaataa aatttgtgaa tgttgtatac ccccaaacaa tacacatatt ttgaaggatt
       61 aggattaata aagctaaaaa tatagtaagt atactaaata tatttactat atctagtata
      121 cttactatat tttaatcttt tgatcgtcta ttatatataa tccgttcctc gtcgtttaaa
      181 gtatcaacat aggcgtccat tgcaagtttg actatttcat acataggaat atcttttgca
      241 taactaagat cccgaatggt ttttagtacg ggaggatcta cttgaactgg ttttctttct
      301 ttcgcagtgt actttcgttc ccctaactcg acattaatat catctttaat tgaaatatct
      361 tcagcaccaa ttttcttctt atctttgttc gttggtaata atgccattta gtttaatctc
      421 ctttccaaga ggtaggatat gattggccat tgagtcagca tatttaggtg tataatcaaa
      481 tccttcaata tcaccatgtt gttcatagta ttcaattctt tcttctaatt ctgtaaagat
      541 gtctgcaaaa atagcccacc atcttcgatc tacataatca tttaattgga taccattctc
      601 accatacatt tctaaccgat ccgagccttt aacaatgctt ttaaaaatgt ttgattcacc
      661 atacttatca attgtttttt gatagtttgc gtgttgagag gaacgtcgca ctgagaaaag
      721 aacaggcaaa attccaatga tttgaagatg agactcttca aaattcgtga caatcggaac
      781 taaaacttta ttaataaaat cttctgttcc ttccattgcg taccgtttta attcttgcat
      841 tgcaataata taatctgcag cagttaagaa tgcacgaacc acattatctg ttgatggtgg
      901 gcagtcgata aagatataat cgaaattttt tctcaattct tctaaaggtt taatgaaggc
      961 caatgatctc ttacgacgat caggatattt ttcggacaag tattcattaa gttcttcaga
     1021 tccaggagag ccagccataa tatataaatg tggacttact tgagtaatac ctgaaaccca
     1081 gtcccctttt ttgattgctg cagacatgga tcgatcaaag ctagtaattt gatacgttga
     1141 tccaatgttg gaagtagtat ttctttgttg gtcataatca attagcaaca cgtctttttc
     1201 aaaaagttga ctagaaacaa tcgctaataa atcattgtct gtagtcttac caatgccgcc
     1261 ttttctacct gtaagcatta caacttttcc tagtttatct gatgttttaa atgcagtccg
     1321 taagttttcc atagttccct ccaatttcct atactaaata tatttagtat acttcatata
     1381 cttaatatat tcgatatatc taatatactc caaattgata agaattcaga ctttataagg
     1441 aatatagtgg atatactaaa tatatttgct atattcagta tatcagatat attcaaaatg
     1501 tcaaaatata tttgtgcgtt ttgcgttgac aatttagagt gaaaagaggt aaacttaatt
     1561 ctatagaaac cacagataat tcactattta caaagtagca aaagcagtgg tactctttaa
     1621 acagaaaaat tacataaaaa aaagagacgg aaataattga ttttagcaga atcaattatt
     1681 tctgaatcac atggttagga catgtgttca cgctcgtctc aagaatatgt aagtagctac
     1741 caactactta tattaaaagt tgatcggata ccttttccga ttgcctttat tctaccaaaa
     1801 aaaggcgagc aactcaacag aagaacgttt atttagtgta cttaaattaa atattcttaa
     1861 acactgaaga gagaacacat actatggctg agattaaagc taaactgtgt tctctttttg
     1921 tttgagaaag gacgttacaa tcgatgttga gcgaaattta ttcagctatt cttaaaaacg
     1981 gcttacgtac atacaaatat cgaaacagca ccatgaaacc tgcaggtcat attgattcca
     2041 gtaggagtgg tgctatcttt gcgtaccgta gcaagcaatt aatgaatgtt gggcgtggta
     2101 tggtcattac ttcggaagaa gctattttag agaacgaaaa gaaactaacg cattggacac
     2161 ctaataccta tcgctttggt acttatgcgg atgattatcg aacagtggtt aaaggtcatt
     2221 ctgagaaaaa cttaagtcaa atcaacacat ttgtagttga tatcgattca aaagagaacc
     2281 atcaaggcga aattatttta gcctgtttag atcaagtggg ctacatgcca acgctaattt
     2341 tagaatcaga tcatggttat caagtttatt ttgtactaaa gacaccaggc tatgtcacaa
     2401 agaaaagcaa tttcaaggtg atcgatgtag caaaaaaaat ttcaaaaaca attcgtctac
     2461 agttggctga aaaattgtcc ggagtggatc taggatgtaa ccacttcgga attgctcgat
     2521 tcccacaaga aaataatatt gtttttaaag aaatgaacta tcagtattct tttgctgact
     2581 ggattcaatg gtcgatgaag cttgaatcag atcaaaaaac aaaagaagat cgagaacgaa
     2641 aactaattgt ctttcctgag aaaaaagaaa ttcgtcaagt agatgaaccg tggttcgata
     2701 tgttacttca taaagcaaac atcgtcggcg gagaaggacg tcttggtcga aataatgtga
     2761 ttttcacgct ttctttagct tattactctt caggttatgc attggatact tgcgaataca
     2821 atatgttcac attcaacgaa cggttacagg aaccgctatc tgaagcagaa ttagtaaaga
     2881 ttgtacgtag tgcttattct ggtaattatc aagcagcaag cagagaattc attattgaat
     2941 tgtgccagca atgggtttca ccaaccttaa aaaataatga tctgttcatt caacgacgtg
     3001 gctggtggaa atttaaaaag ccacgagatc aaagaaaata tagtcataaa aatgaatgga
     3061 aagaagatct attggcttat ctaagcgaaa aaagtcttgc tagtgagcct tatttgaacc
     3121 tgtcaaaaaa agaattggct tcacaactaa cgatgcccaa gcgaacactt gataagatct
     3181 taaatgagtt acgagaggag aataaagtct tctacaaggt aacacgagga agaaatggcg
     3241 gaatgatcat tgcttcagtc aaaacgttgt tcgcgagggt tattcaacta agtaaagaaa
     3301 agagactaga ttattcaaca aggatcacag aggtatttgg actcaacgag agcttagtag
     3361 aagagacatt tagacaatta tcaaccccct tgaaccctct taaagaagtg aatctttttg
     3421 aagtagatac aggttgaaaa ataaaaggtg caaattgccc tatacaatat atctatgata
     3481 cttctgtagg tgttgctccc tttgcctagc tacgtgtagg tggtatactt ataactgcgt
     3541 atgcagtctt atgtatcatg gaggaagttg tatgtcgtta aaaaattatg tctattcaca
     3601 tcctgttaat gtgtttatca ttggtcgttt gggattatct gtggaagtgt tctgtgagct
     3661 ttatggtttt aagcagggga ctttgtcttc ttgggttacc agggaaaaaa cagttgcttc
     3721 tttgccgatt gagtttcttc atgggttgtc cttagcttct ggggagacta tggatcaggt
     3781 atatgattgt ttgtgtgtgt tagagcagga gtatattgag tataaaatag ctaacgagtt
     3841 aagaaaaagg aagaagtata tccagtagta gtttttattt acttaaaaaa ttggttcatt
     3901 gagcaaccta tcgctgctaa agtagcaaag gaaaacgtta aaggaaagaa tttagggaag
     3961 aaatcgaaat gaaatcggac gactggattg aacacacctg gcttcctctg aaaagaaata
     4021 gcaaacgaaa gaaattttgg gtattgcaat tatggccatt aggaaaactt tgtagttacg
     4081 gggcacgact tattcttgat ttaaccgttt actcgattgg agtatttgga acggcttatt
     4141 acttgataaa tgatcccaat aggattgcag catcagacat ttttacagtg ctaatgacgt
     4201 tgatttttgt catgctattg tctattttta ctcaattagt catgcaattt accaggcaaa
     4261 aattaacagc aggaaaactt ttcaaaagcc tattcgattt aattgttgta aatgcaattc
     4321 tagcgacttt aataaaacaa attgaacaat ctggaagaac aacaaaagga gtgaaggatt
     4381 ttttattgac gtttattcac tttgtaaatg gacacatgta tttgtttgaa gtgggtatta
     4441 ttctcttttt tgtcgggtat gtatccacga aacttattaa acgttcaatt gaaaaagaaa
     4501 ttgaaattga cctgattccg acacttcttg gttatgagta caatgttgat tttggaagat
     4561 atgacgaatt gatcaatacg gatatcgttc acacgagtat ccatgaatct actgttaagg
     4621 gcatcagatc tattttaaaa aatggccagg aaatgaaagc aatagatcga agaaaagaga
     4681 tctacaattt ttatgaagtc gaagcaagcc tacgatatgg catcggaaaa ttgtggttag
     4741 atggaagtaa tattcgtcaa aacaaaccat acattttttg gaacagtagt gatttagaac
     4801 ttccaatgaa agataaataa ttatggagaa tcgtttgaaa atgattcctc tatacaggat
     4861 tgtgcttata gttggcatgg agttaaaaac cttaaagaag gagcagttat tcaaagtgaa
     4921 agaacgtcat aatcagttac tatgtagtaa aacgggtacg atgaacattg aataccgaac
     4981 agaatattta gaattaacga atcaaactga ttttttgttg attcgaaagc aacaagagca
     5041 agaaaaaaat taatggctac ttatagccaa ttgattgatg aatgggaaca attcaaggaa
     5101 tctaaaattc cagaacgtct ctaaaagcct ttatttgtaa agagcataag gaaacgtggg
     5161 aacacttttt aaaaaaagtg ttccaatagg ctgtgcctat tacgtttctc aaaaataaat
     5221 gtgggaacaa aatatttaaa aataggatag tgaaaggata gtgaaggata gtaaaaggag
     5281 ggtagtagga tagcaaaaag gataggctcc ttttcgctat cctttttaaa ttggaggaat
     5341 taatatggaa gtaagaataa gaaatgtgga tgagaaaatc gtaagagaaa ttgatcgaag
     5401 atgtgac
//
LOCUS       DAZKOW010000134         5399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000134 DAZKOW010000000
VERSION     DAZKOW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.138"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..1022)
                     /locus_tag="ACG912_002659"
     CDS             complement(45..1022)
                     /locus_tag="ACG912_002659"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFQ0513928.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="ACG912_002660"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="ACG912_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFQ0513929.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            2296..2553
                     /locus_tag="ACG912_002661"
     CDS             2296..2553
                     /locus_tag="ACG912_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513930.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            complement(2595..4019)
                     /locus_tag="ACG912_002662"
     CDS             complement(2595..4019)
                     /locus_tag="ACG912_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFQ0513931.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(4283..5260)
                     /gene="guaC"
                     /locus_tag="ACG912_002663"
     CDS             complement(4283..5260)
                     /gene="guaC"
                     /locus_tag="ACG912_002663"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFQ0513932.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         1501 a         1150 c          923 g         1825 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaacattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaaatt taaatccaag ctgttatatt ctttaatcta
     2581 tttactttgt gaaattattc tgttaaccac ttttctagaa ttttcatctt tgaataaaac
     2641 aacggtattt ggttttcatc cagcctactg taattgattc tgattccatt tatatcttgc
     2701 ccaaaaagta gacttggtgc aattccaaga cccatatcta ctactttttc tgctagttcg
     2761 gggtctagtt ttttcccatc ccaagtaatc caggcatagt aaccacctaa aattgaatga
     2821 agcttgaaaa acgattgtga atctccccaa gcgcttatca gtcggctttt ttctttcaac
     2881 ttttttctta catcttgcat tttcaaatca attgcttcat taaatacatt agccactgtg
     2941 atttgtgtaa ggatattcat cgaaaactcc atcatttctt gcgcttgagc tagttgcgag
     3001 ataaaagtct caggtcctat cacccaaccg attttcgttg tttttcctaa aaccctagat
     3061 aacgaaccga cgtataacac attatccgga tcaatcgttc gtataggaag gacttcttct
     3121 tggtttagaa atgaaagatc cgtaaataca tcgtcttcaa taattggaag ccgatactgt
     3181 cgacacaatt ctactagctt ttctcgtcgt ttttgactca tcacttttcc tgtagggttt
     3241 tgaaaattcg ggttgataag cactgctttc aatttatttt tatggatcga ttctttcagt
     3301 ttttctaagt cgatcccttc ttcatccatc ggtatcccaa ataaacgcgt tcctgaagct
     3361 ttgaaaagag gtaatcggta aaaaaaagaa ggtgactcta cagctatccc ctctccagct
     3421 gaaagaatcg tttgtaaaat caaaaagatt gcttgctgac cgccaccagt cacaagaagc
     3481 tggttggggg aaaatgccaa ttcaaattta ctttccaaat accgtttgat tttgttacgt
     3541 aacggagtat agcctaaaag atccatttct ttttcttccg ctaagatttc ttcaagagaa
     3601 taagaaggaa actcaaaatt cggcaaaagc tcatctggca tctcgctagc gtataaatca
     3661 agaaacgatg ttttttggat ctgctttgct tctcttattt tttgctcata ccagtccgtc
     3721 tgctcataac gaggagagat catttgccgc caatcgattc ttggaacagc gaatttcccc
     3781 cattcagtct gattaacaaa ccatccgctg ccacgtttgc tcgttagtac cccaagctcc
     3841 ctaagctctt ccaatgcatg gacaacggtg gaacgattca caccaaaaag ttgccctagt
     3901 tttcgttctg attcaagttg acttccagga ttgatccttc cgttttcgat ttctctcata
     3961 atctgttcca taatttgacg ataaatcggt ccttgttttt tctctacttt ttcccacata
     4021 agcttctctt ttctattgtc tattttttct tttgatttta ccgaatttac gagaaaaaag
     4081 aaaaactttt tttgcaaaac ataagggact gtgacaaaaa tcttatcaca gtcccttatt
     4141 ctgaataatg atgttcaaaa gcttgtatct gcgataataa ttccagccaa acaaaacata
     4201 taaagaacta tttttgtttg gctgttctta ttacttaatg caggacaact ttttcacaac
     4261 atcctaatat tttatttatt ttttagattg tgtctccatt aaaaatcgaa tttttcacga
     4321 tgacatagtc aacttttcga atggcttcca gatctcttcc tccagcataa gaaatagatg
     4381 attgaaggtc ttgttgcatt tccactagtg tatctttcaa agatcctttg tgttggatcc
     4441 agattttctt tccttcaaca tttcgttttt cgcctttttg gaattctgaa gcagaaccaa
     4501 agtattcttt atacacgatt ccattttcga cttttgtttc gccaggagat tcctcgtgac
     4561 cggcaaacaa tgaaccaatc atgaccattg tcgcaccaaa acggacagat tttgcaatat
     4621 cgccatgtgt tctgatccca ccgtcagcga tgatcggctt gcgagctgct ttcgcacacc
     4681 atctaagagc tgccagttgc cagcctccag tcccaaagcc tgtcttgatt tttgtaatac
     4741 atacttttcc tggaccaatc ccgacttttg ttgcatctgc gcccgcattt tccaattcac
     4801 ggacggcttc cggtgtacca acattaccgg caatcacaaa agtttcaggc aaatattttt
     4861 taatatgctg aatcatatca ataactgagt tagcatgtcc atgcgcgatg tcgatagtga
     4921 tataatcagg atttagcgat tcttctgcca acttctctat aaaggaatat tcctctttct
     4981 tgacgccaac actgatcgag gtaatcaatc cacgcttttt catttttttg ataaacggaa
     5041 ttcgagctgc ttcatcaaac cgatgcatga tatagaaata accattttcc gctaagaatt
     5101 ctgcgattgt ttcatcaata atcgtctgca tgttggcagg cactactggc attttgaatg
     5161 tatgtttacc taatgtgacc gttgtatcac attcagaacg gctatttacg atacatttat
     5221 taggaattaa ttgaatatct tcgtaatcaa atactttcat tacattatcc tcctgatgtt
     5281 cacatccagc aaaacacgaa cattattaat ttaatcaagg ctttttttag cgccctatgt
     5341 aatttaactc aattaagcaa aaaaatcaag agcaaaaaga gaatttatac gaatggtaa
//
LOCUS       DAZKOW010000135         5313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000135 DAZKOW010000000
VERSION     DAZKOW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.139"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG912_002664"
     CDS             complement(74..1222)
                     /gene="nagA"
                     /locus_tag="ACG912_002664"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFQ0513933.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1298..2134)
                     /locus_tag="ACG912_002665"
     CDS             complement(1298..2134)
                     /locus_tag="ACG912_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513934.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2318..3535)
                     /locus_tag="ACG912_002666"
     CDS             complement(2318..3535)
                     /locus_tag="ACG912_002666"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFQ0513935.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3684..3845)
                     /locus_tag="ACG912_002667"
     CDS             complement(3684..3845)
                     /locus_tag="ACG912_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513936.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3918..4001
                     /locus_tag="ACG912_002668"
     tRNA            3918..4001
                     /locus_tag="ACG912_002668"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3952..3954,aa:Leu,seq:gag)
     gene            complement(4241..5313)
                     /locus_tag="ACG912_002669"
                     /pseudo
     CDS             complement(4241..5313)
                     /locus_tag="ACG912_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1739 t
ORIGIN      
        1 tttttactag aacacaaaaa aaaccttaaa accaaactta agaagtttag ttttaaggtt
       61 aacttaaatt atattatgcg tggaaacgtt ccacgccatc tagataagtt gcttccaatg
      121 tcatatcagg gttcaatacg ataaagtctg catcgcgtcc ttgagcgatt tttccacatg
      181 tatcatcgat tttacagctg attgctggaa tcagactagc catcatgact gcttgttctg
      241 gtgttgctaa tccccagtct acaacatttt taattgcttc tttcaatttc aatatacttc
      301 cagctaagtt gccagattct agacgtgctg ttccttcttt tactactact ggaaattcgc
      361 ctaaattgta atttccatca ggcatcccac cggccatcat acagtctgta attaatgcga
      421 catgatcatg tcctgctttt tccattagga ttcccgcagc atttggatgt acatgatggc
      481 catcacaaat caattcagaa aagacatgtt ctaaagataa taacgcaccc accatacctg
      541 gttcgcgatg attcaatcca cgcataccat tgaaagcatg gacaaataca cttgcgcctg
      601 cttcaacagc tttttgcgct tcttctagtg tagcattact gtgtcctaga gaaacaacta
      661 ctccttcttc agtcacttgt ttgacaaact cttctacacc ttgacgttcc ggtgctaaag
      721 caattttttt gattaaacca cctgaagctt cttgccattc atggaaagta tccaaatcag
      781 gatccccgaa ataacttggg ttttgtgccc ctttgtgttc ttctgtgaag aaaggacctt
      841 caaaatagat tcctttgatt ttagctcctg gtgcttcctg atacattttg ccgattgtct
      901 cagctacatc ttttaaacgt tctttgcttg acgtcaatgt tgttggtaaa aatgaagtca
      961 ctccacaaga taacagacct tcagacatca ctttgatgcc ttctgcatca ttatccatta
     1021 catcatgatt catatagccg tgaatatgtg tatctaccaa accaggagca atccatttgc
     1081 cgctttggtc aatgatgttg acttctccct caggtagttc tttggtgtat tcaccaaaaa
     1141 gtccatcggt aatttcaaga tagcctgcat gtttgacacc tgacgctaaa aagaatttgt
     1201 cagcatagat aaaagttctc attctattct ctccctttgt ttttctacct ctaaagtact
     1261 cttatttaga gaagaagtca agaatggtct acaccattta caaatttttt gaaatttgtt
     1321 tcacagcttg tgctaagcct tcatagaatg ctgctctttc tggttcttct ttacgcttgt
     1381 atattaagct tttaagcaca aataaatcat tgaggtaata gtagctattc ttctcttgag
     1441 cccaatagat tccttgattc acctgttcta acgcttcgtc gatttcattt tgatggaata
     1501 agaaatttcc ataattataa aaaatttgcg aaaattcact ttttgatcgt tcatcagctg
     1561 attgatgcag cagttggatg cttcgactca aatagtagcg tgcttcagcc gtttttcctg
     1621 ccacaccata tatttttccg atacaactaa tcagttgtat ttctgtgtca gaaacatgga
     1681 ttctttcttt ttggctagag tagcttaatg cttctttcaa ataataaagg gactgagaga
     1741 tattttgacg caacgtgaat tcgcaaacac cgagataata ataatagcgt tgaaagtcag
     1801 ttgcttgata cagattttca gaaatatcag aagattttag tacttgttct aacttccgat
     1861 aatcacgttt ttggaaacaa ctatccaaca gttcaaataa tttatcgttt gtccgagtag
     1921 tttttcgaga gagattcatc acatgatcaa tcgttacccc tagccgttga caaatctgca
     1981 tcatcaccaa tacattcggc agttcctcat tattttcgat ccgacttaat acactttgtg
     2041 aacaaatatc atgcgaaagc attttttgcg tcattttttg ttcttttcga atctctttaa
     2101 tgatcgttcc aaaggactcg attaacgttt ccataagtat cctccccaaa atatgcgtat
     2161 tcgcattcta ttcgtatttt actttaggaa gtcgcaaact tcaagaaaag acttgcaact
     2221 aaataatata ctttagttat ttaagaaaac tccactagaa agagagcctc agtgtgtttg
     2281 tctgtggccc tcctactgaa aaatttggtt tttaaaatca tttttggcga taggttgcta
     2341 ggaattctct cattttctta gctgtttgtt tcaaatcatc cattttagga agataaacga
     2401 ttcgaaaatg atctggctgg ttccaattaa atccaccacc atgtacaagt agaatatgat
     2461 gttcatgtaa aaagtccaaa acgaattttt catcatccaa aatattgaat cgctttgtat
     2521 caatttttgg gaatatataa aaagcagctt ttggttttac ggctgaaaga ccaggaatat
     2581 cattgattgc attatagata tattctcttt gttcatagat ccttccgcct ggcaggagca
     2641 gctcatcgac actttgataa ccgcctagcg cggtttggat aatttgctga gacaatacat
     2701 tggaacacaa acgcatggag gacaacatat tcagcccttc tatatagcct ttgacgtgtg
     2761 acttatctcc acttaggacc atccagccta ctcgaaatcc tgctacacga tgagatttgg
     2821 atagaccgtt cagcgtaaca acgaacaaat caggcgctaa tgtagcaatc ggaatatgtt
     2881 ctagaccatc catcactaag cgatcataga tttcgtcgga ataaataatc aggttatttt
     2941 gtcgagctac ttcaacgatt tcttctaata attcttttgg ataaagtgcc ccagtaggat
     3001 tattaggatt aatgataaca atagcttttg tccgactcgt tatttttgat ttgatatcat
     3061 caatatcagg ataccattct gcctcttcat cgcaaatata atgaacaggt gtgcctccag
     3121 ctaatgcaat agaagccgtc cacaacggat agtccggcat tggaaccagt acttcatctc
     3181 cattgtccag caacccctgc atacacatcg tgatcaattc gctcactccg ttgccggtgt
     3241 agatatcatt gatcgtcaca ttcggaaaat gtttcagttg gcaatattgc tcaattgctt
     3301 ttctagcaga aaagatcccc ttggaatctg agtagccttc tgagtcacgg gcattcatga
     3361 tcaaatcacg aatgacttcg tttggtgctt caaatccaaa aggtgcggga tttcccgtgt
     3421 tcaattttaa aatactaatg ccctcttctt gcatccgctc cgcttcttct agaacgggac
     3481 cgcgaacatc gtaactgaca ccttctaatt tgttggattt ttcaaattct ctcatcactt
     3541 agccaatccc tcatttcaaa aagttagaac tttccaaatt ttttgttatt tactatttca
     3601 atatcatact ccatcctaga aaattttgcc acttatttag gaaaaacgta agtattaaaa
     3661 aaacacaaac aagcgttgag aacttaattg tcatgatagt cattccatag attcgctgcc
     3721 caatccattg caattccaca aatgattcct agcataaaac caagcaaaaa acgtccgaat
     3781 gcaagtccaa tcaatacacc taagatcatt cccagtcctg aaaattctcc gtcatatttc
     3841 tccatcttaa taagtcctcc catttttgag cttgacaact atgtaaaacc tcgctatcat
     3901 tagtgtgtta ttattacgca gatgtgttgg aattggcaga caagcatgat tgagggtcat
     3961 gtgagcgtta gctcgtgtgg gttcgagtcc catcatctgc actacaactc gtgaagttaa
     4021 ttttgcttca cgagtttttt tgtttgaacg tttctttatg ccattatata catatcactg
     4081 tatcctttac aaaaagaacg tacaatcatg taaacagcct gaggtgagta tatgaaggtc
     4141 ggaaacaagc tactcgtaga aacttgcaag tgttaaaaca attaatcgaa aaacgagtaa
     4201 aatccctcac ctaatactaa agcgagggat tttttgtata ttatctgttt atcaccgtta
     4261 ttataaaggt acaaatacca agcacggtta atccaaattg cataaattca tttttttgta
     4321 cactcatgct tttcattagc tctttaactg ccattagtac taagatggcg ataacaacat
     4381 aaagtatata gtacgtccgc attccggaag cccatactgt gggagaaaat cccataatta
     4441 accttgaaca aaatcccatt atcatcaaaa taactaaaaa aatagaagtt ttcttattgt
     4501 caaaacttaa ataaattcca acaattaaac atagtaataa tgccaagact actaaagtag
     4561 ctatccaagt acctggataa aaaagtgaca gtttcgttcc taactctgta aagagattat
     4621 tcaggttact actactccaa atcattgccc ttttatttcc attgacgtaa gtaaagcttt
     4681 gacccattgt attaccaaaa taaataatca ggtttaagaa aaacggtaat gcagtcaata
     4741 tacgcacata atagttttga catttcctat atgttagaag gaaaattaag aaaaatagca
     4801 ataaaaataa gatattcatg tctaaaaaaa cggctttcca aatgatgaaa tacctaaatc
     4861 taacttatta agaaagttga aatttttata ttcaggaaac catttattaa tttcatgtgc
     4921 agcacgtaat gcattccctg gagttgtcaa agagaaaatt aattctgcta agcttattat
     4981 tgaaaatact gataacttat aattatatcg tccattgaca atcaaatata aactaactat
     5041 acttgttaga acaaaaaaac aggcgttgac ttgttcttga ttagttaaaa atatcaataa
     5101 aggaatactt acccaatata tttttctatt tatttcttct cctcttaaga gttgaaaaaa
     5161 cggataaaaa gttagtaaac taaatgctac tggccactga tagttcaagg tggttgctct
     5221 ccaaccggtt tcccccatta ctgtcaatgg tattaaacag tatatagagc aaaccaataa
     5281 taaattttta acatttattt tctcaaaaac ata
//
LOCUS       DAZKOW010000136         5189 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000136 DAZKOW010000000
VERSION     DAZKOW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.140"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..748
                     /locus_tag="ACG912_002670"
     CDS             137..748
                     /locus_tag="ACG912_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0513937.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="ACG912_002671"
     CDS             821..1750
                     /locus_tag="ACG912_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFQ0513938.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1887..2645
                     /locus_tag="ACG912_002672"
                     /pseudo
     CDS             1887..2645
                     /locus_tag="ACG912_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
     gene            complement(2693..3895)
                     /locus_tag="ACG912_002673"
     CDS             complement(2693..3895)
                     /locus_tag="ACG912_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0513939.1"
                     /translation="MHRKEDFHMNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKT
                     ELTAFLDYEKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYR
                     RTNDTLEETVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERE
                     LHDRYAAIYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEI
                     LIDLQERGVKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKE
                     VCDDFKMVYQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKT
                     IYSTNLIESFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTD
                     TLASMFTE"
     gene            3975..4499
                     /locus_tag="ACG912_002674"
                     /pseudo
     CDS             3975..4499
                     /locus_tag="ACG912_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4706..>5189)
                     /locus_tag="ACG912_002675"
     CDS             complement(4706..>5189)
                     /locus_tag="ACG912_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFQ0513940.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
BASE COUNT         1639 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttt
//
LOCUS       DAZKOW010000137         5176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000137 DAZKOW010000000
VERSION     DAZKOW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.141"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..1098)
                     /locus_tag="ACG912_002676"
     CDS             complement(136..1098)
                     /locus_tag="ACG912_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFQ0513941.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1100..2539)
                     /locus_tag="ACG912_002677"
     CDS             complement(1100..2539)
                     /locus_tag="ACG912_002677"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFQ0513942.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2724..4670
                     /locus_tag="ACG912_002678"
     CDS             2724..4670
                     /locus_tag="ACG912_002678"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFQ0513943.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4934..>5176
                     /locus_tag="ACG912_002679"
     CDS             4934..>5176
                     /locus_tag="ACG912_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0513944.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1666 a          878 c          855 g         1777 t
ORIGIN      
        1 tttctccact ccaaaaaagt ctaacttttg ggggtcacta caagttgaca actccttttt
       61 gtttacaaat caattggata tctacaaatt gtattttcta acgaatttta tagacattaa
      121 aaaattaatt taacgttaaa tacttcctcc agaaagtatt gagattggta aaatataatc
      181 cttatttgtc ttttcgcctt taattttttt cagaagaata tcaacaataa gacttgcaat
      241 ttcatcaatt ggttgtctta ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt
      301 accatcgtat ccaataattt ttaaatcttc tgggatatta agtttaagtt gatgagcaat
      361 tttcatagtg agaatggctg ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt
      421 tataatagtt gactttattt ccatttcacg acggatagta gacagattat taggaatttt
      481 gaaaattttt ccatttggat tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga
      541 atccgtgtta tcatttcctg taatcataat ggtattttga caaccatttt tttgtaaagt
      601 cttagctgca agttttcccc cttcaaagtt atctgaagaa acaatggaaa cattaggagc
      661 taaatttcta tcaaaagcaa caataggtgc tctcacacgc tcataatcag caattccaag
      721 attatggctt gatgagatta tgccatcaac ttggttggct tcaagcattt caatgtattc
      781 tctctcttta gcaggatcat tttgactatt gcaaataatc gttttatatc catgtttaaa
      841 taactctatt tcgagatact caatgagttc agagtaaaag atattactaa tattaggaaa
      901 aataagacca ataagctgag gtgactttcc ttgaagtcca cgtgctaaat tatttggttt
      961 ataaccaaga gttttcatag cttcatgaac tttttgtttt gttttttctg ataaatatcc
     1021 cttattatta atgacgcgtg aaacggttgt tgggctaact ccagctaatt cagcgacatc
     1081 agttaatttt gctaccattt taatatttta attcaaagta ttttccagta ggttcgcctg
     1141 atttgatttg aataccattt tggtgatttt cagggaatac acgactagta aacacttttt
     1201 ctcctttatt aataaaaatc tcaatgattg agttatctac aaaaatgtta gcagttgttt
     1261 ctttagtatc aattgagcat tcacgaatcg taccaaagtc agtggcatat tgaatgcctg
     1321 ctttgctgcg atctaaaata atttttcctt ctttggtatc tacaatcaag ctaagtccat
     1381 ttcctttact atcagagaaa agaagtaact ccgatttctg atttggagga aaagttagtt
     1441 caagctcata agtattattt gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta
     1501 atgatgtaat agcttctact ggatattggt aaagtttacc atcttttatt gaaagttctt
     1561 ttacaagact cattgctccc tgataatcat acttatcagt tggataatcg atatcaggta
     1621 gtccaatcca actgacaatc agagcacgac catcaggagt attgaatcct tgagtcgcat
     1681 acgcttcaaa tccataatca agattttgaa tttctgaagc gcccacaagt tttacttttt
     1741 ctatgtcaag cgattgacag atcttaaaag tattcggata gatattccca taatttagct
     1801 cagatttatc caacccttga ggacaataaa tgagaacagg ttttttgtct acaaatacta
     1861 gatttggaca ctcaatcatg tattctgaac cagtgccgcc aaaatcaagg ttgccaactt
     1921 cctcccatgt ttccacgtta ttgtcatccg ctttgtaaag tttgataaag cctgattttt
     1981 ctaaattttg tgctccaaca atcgcataca attgtccttc ataattaaac atttgtggat
     2041 cacgaaaatg atcagttaca tcagcaggtt tatgaataag aacattctca cctttcgtaa
     2101 tgttgtagtc tttatccatc catgctccga tttgaagtgt atcacgttcc caattttcat
     2161 ttcgaacgtt tcctgtatag agtaagaata gattcccctc tatttcatat gcagaaccgg
     2221 aataagcgcc gtggctatct cgttttgtgt ctggaagaag ttctgctcct gttacatgaa
     2281 aatgaactaa atcatctgat tcattgtgta cccattcttt caacccatga gcagctccaa
     2341 atggccaatt ttgataaaaa agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat
     2401 cgtttaacaa tcctgtttta ggttcgatat gatagctagc acgccaaggt gatcgtttta
     2461 cattatcctt aattttatcc atctcatctt tagtccaatc agaataagca cgatatcgta
     2521 tttctcgagg taagttcata ttttactcct tcaatttatc tttttacaac tagtatggta
     2581 aacgttttcc gttgaaaaat caatcatttc atttaaaaaa ataaactata tgacaaacgt
     2641 ttgacatatt tttaagatat gctaaaataa atttgcattt cagtgaaccg ttttcacgta
     2701 aaaataaaaa aggagtgatt cgaatgaatt acagtaaagt agcttccgac gtaatacaag
     2761 cggtcggaaa agataacctt attgcagctg cacactgcgc tacacgtctt cgtctcgtac
     2821 taaaagatga cacgaaagtg aatcaaaaag ctttagacga aaatccagat gtgaaaggca
     2881 catttaaaat tgatggacaa tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt
     2941 atgacgaact tatcaaaaaa actggacttt cggaaatttc aacagatgat ttaaaacaaa
     3001 ttgctagtaa agataaaaaa ttcaacccca ttatggcatt aattaaatta ttatcagata
     3061 tttttgttcc aattatacct gcccttgttg caggtggttt attaatggca ttacaaaact
     3121 ttttaacagc tgctggactt tttggaccaa aatcaattga agaaatgtat cctgcgattg
     3181 aaggtatttc ctcaatgatc caactgatgt ccgctgcacc attcatgttc ttaccaattt
     3241 tagtaggtat ttcagctgct aaacgttttg gagcaaacca atttcttggt gctgcaattg
     3301 gtatgatcat gacaacacca gatcttggtg gctcaactga atattggaat atttttggtt
     3361 atcatgtttc acaaacaaac tatgcttatc aagttattcc agttttagca gctgtttgga
     3421 tattatctat attagaaaaa ttctttcata aaaaattgcc atcatctatt gactttacat
     3481 tcacgccatt attatcagta atgattactg gatttttgac tttcacagtt attggccctg
     3541 taatgttatt gttgtctaat ggaattacag acattattgt ttggctatat aacactactg
     3601 gatttattgg aatgggtatt tttggaggaa catattcact tatagtcatg actggtttac
     3661 atcaatcatt tccagctatt gaaactcaat tactttcagc gtggacaaat ggtatcggac
     3721 atggagactt tatctttgta gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat
     3781 ttgccatttg gttcttaact aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg
     3841 tctcagcact tcttggaatt acagagcctg ccttgttcgg agttaactta aaataccgct
     3901 ttccattctt ttgtgcttta attggttcag gaattgctac agcagtagca ggattactta
     3961 aagtagttgc tgtttcactt ggttcagcag gtttcttggg atttctttca attaatgcaa
     4021 catctattcc attttacgta ttgtgtgaat tgattagctt tgtagttgct tttgcaatta
     4081 cttacttcta cggtaaaaca aaagctgctg gtatctttaa agcagaagaa attgctgaac
     4141 aaacaccagt taattcttca aaagtagcaa caacagatcc aatatctact gatgttgaat
     4201 caacacaaac aaaagtttca tcagaaacaa ttgttagtcc catttctggt aaagtgatta
     4261 gtctttcttc agtaaatgat ccagtctttt catcagaagc tatgggaaaa gggattgcca
     4321 ttaaaccaaa tggaaacacg gtccattctc cagtagatgg tactattcaa ctcacgtttg
     4381 aaactggaca tgcttatggt attaaatcaa ataatggtgc tgaagttcta attcatattg
     4441 gtattgatac tgtatcaatg ggaggaaaag gatttacaca aaaagttact actaatcaaa
     4501 aagttagaaa aggcgatatt cttggagcat ttgaccgttc aatgattgag aaagaaggtc
     4561 ttgatgatac aacaatgatt attgtgacca atacttcgga ttattcagaa gtaaatatag
     4621 tagctgaaaa tacagtcact gagggtactg atttacttga aatcaaataa atatatttcc
     4681 ctgagtaaag atggaatttt ctttccaaaa tcaaataata tagattaatc aaatatttga
     4741 gaataacatt ttatttaatc ttttcatata tcaattgtat gaaaaattat tagaatttta
     4801 aaaaatgata aaaattcaaa aagaaaaaat agtagttatt tacttgtcat gttactaaat
     4861 aaaaaaataa tttaaatatg atatatagtc atttatttag tgatttcatt ctaaaataaa
     4921 gggagaaatg tctatgctat acggtgcaat tgaagcaggc ggcacaaaat ttgtgtgtgc
     4981 cattggcgat gaagaaatga cgatcaagga aagagtgagc tttccgacaa cgacacctga
     5041 ggaaacaatg cctttagtca ttgacttctt caaacaatac caagcagatt tagcagggat
     5101 tgggattggc tcatttgggc ctatcgatat ccatcgcgat tccgccactt atgggtacat
     5161 tacctcgacc ccgaaa
//
LOCUS       DAZKOW010000138         5163 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000138 DAZKOW010000000
VERSION     DAZKOW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.142"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1371)
                     /gene="uxaC"
                     /locus_tag="ACG912_002680"
     CDS             complement(<1..1371)
                     /gene="uxaC"
                     /locus_tag="ACG912_002680"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFQ0513945.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENI"
     gene            complement(1445..2524)
                     /gene="uxuA"
                     /locus_tag="ACG912_002681"
     CDS             complement(1445..2524)
                     /gene="uxuA"
                     /locus_tag="ACG912_002681"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFQ0513946.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(2527..4158)
                     /locus_tag="ACG912_002682"
     CDS             complement(2527..4158)
                     /locus_tag="ACG912_002682"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFQ0513947.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            4462..5133
                     /locus_tag="ACG912_002683"
     CDS             4462..5133
                     /locus_tag="ACG912_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0513948.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         1651 a         1025 c          731 g         1756 t
ORIGIN      
        1 aatattctcc actatctgac taagatacat ttcatcatct ggaattcggc ctgctttaac
       61 ccactcgctt agtaattgac ataatactcg acgaaaatac tcatggcgag gatagcttag
      121 aaaacttcga gaatcagtca acatacctgt aaaattcgac aacaaacttt gttgtgccat
      181 cgtagtcaac tgttgacgca ttccttcagc tgtatcattg aaccaccaag cacatcccaa
      241 ttggattttt tgaatcatac ccccttggaa gctttgcatt gctgttgcta gtggaagcca
      301 atcagttgga tttaatgaat ataaaattgt ttttggtaag cacccactct tttcagcggt
      361 atcgaacaat gctacaacat tttgtgtcaa actagcttga tcccccatcg aatcaccacc
      421 gacatctact ccaatttgtt cctttaatat acttgaatta tttcttaaag catttaaatg
      481 catttgcatc gtccaaccat atcttgtata ctcagacatc aaatgtaatt gaatacctgt
      541 aacaaattgt gcaatttctg ataaatggaa atccgttttt cctgctaaag cttttattaa
      601 cagattgtcg agtatttttt catctgctat ttcataaaaa taagtattca aaccgtgatc
      661 tgctaatcga cagccaattt gatgaaaatg atccactcgt tggctcaatg cttgcttcaa
      721 cccattaaag tctttgattg gtatagccgc tgtttgacta agttttaaaa tatagttcga
      781 aaagcctgat gatttgatac tccagacctt atctggacgg aaagtaggta ataccttgaa
      841 agaattatcc tgtttagaaa gctcagtatg ataccacaag tcatctgtag gatcatccgt
      901 tgtacagatg gctttcacat ccatcatctc caacaattct ttcgggcgat aggaatccgt
      961 ttgaattttt tcgttagcac gtcgccaaat ttctggtgca ttcttctcag aaattgtcaa
     1021 atcaatatca aagtaacgtt gtaattccaa atgagaccat tcaaaaatag gattacctgg
     1081 cgccttctcc actgttttta caaaagctaa aaatttttca taatcatcac ctggaccact
     1141 gatcaactct tcttctatac cattagctcg catcaatcgc catttataat gatctccagc
     1201 tcctttatca tttaaccata cctgagtcaa attcttgtat tgcttatttt ctagcaaata
     1261 tgctgggtct aaatgacaat gatagtcgat aattggttgg tgttttgcaa atttatgaaa
     1321 aagtctttta gctatttcat tctctaataa aaagttctta tctaaaaaca tatttaaaca
     1381 ctcctttcct tccgttagtt tttagaagag aaaatttttc ctctaaataa actcccttat
     1441 ataattattt actcgccccc ttttcaagcg cttcccaaat accatttaaa taaactgcac
     1501 ctagagctcg gtcatataat ccataaccag gtctaccggt ttctccccaa atcatccgac
     1561 catgatctgg acgaatatag ccattaaatc cattatcgta cattgttttt aatatcttat
     1621 acatatctag agaaccatat tctgatttat gcggcgcttc gtaaaaatcg agtccattgt
     1681 cacttaaata tttgatatta cgaacatgtg caaaaggaat ccgatcccgt tgacaaaatt
     1741 cagatagaat ttcatagaca tcattatctg gtcgttctgc aatacttcca gtacataatg
     1801 taataccatt agaaggagaa tccactacat tacacaacca atctaaatcg tcacgatttt
     1861 taacaatacg aggtaatccg aaaatggaat atggtggatc atctgggtga actgccattt
     1921 taatcccaac ttcttcacat gtcgggataa ttgctttgag aaaatatgca aagttctctc
     1981 gtaatttctc ttcatcaact tctttatagg cttctaataa ttgcttaatt tgtgataacc
     2041 gatgaggttc ccaacccggc aattcaaaat tattagtaga attttctact tctctaataa
     2101 tttcctccgg atcagtaggg atattttttg aaatataggc taacgtagaa gacccatcat
     2161 ctaaacgata atccaaatcc gattttaccc aatcaaatac tggcataaaa ttataacaga
     2221 taacctcaat cccaaattct gccaaattgc gaatcgtctg tttataattc tcaatgtatt
     2281 gatctcgtgt tggtaaacca attttgatat catcatgaac attaacactc tcaataactt
     2341 tcaaggtaag tccattggct tcgacttcat cttttaaaga tttaattcgt tctctcggcc
     2401 aaacctctcc ggctggtata tccattaatg ttccaacaat tcctttcatt gctgggatct
     2461 gacgaatttc ttgtagtgag attttttctt catttgcccc ataccatcta aaagtcattt
     2521 ccatagttac tttcctccaa cttctgttat atatttattg attgttttac gaactgcttg
     2581 aggacctact agcatttcgc aaaagatttt ttcagttttc tttcctaccc caacttgata
     2641 taaatctaaa ccaaagattt ctttattttc taaaatttcc tgtaccgcac aatgaatctt
     2701 tgaaggatct tgttcaccta atttcaatac tgataattta tcctgcaact ctaccaataa
     2761 cggatcagga cttggcacaa aattttcccc ttgatcatcg actgccagta aataacgtaa
     2821 ccaagcagca atagcgatag gaataaatgt tagattttct gcatttccgt ctttttcgac
     2881 atacttttta attgtttcac catagcgaat cgctactttt tgagaagtat ctgttgcaat
     2941 tcgttgtggt gtatctggaa tatagggatt aggcaaacgt ttattaatta cttcatcaat
     3001 aaattgtttt ggatcaataa tgcctggatt ttcaactact ggtaaacctt ctaaataacc
     3061 tattcctttg attaaaccaa ccaaatcatc atcctgcatc tctgctgaaa ttgattgata
     3121 tcctagcaga caacctgtta ctgccaaagc agtatgaagt ggattcaagc ttgtcgtaac
     3181 cttcatgatg tctgctttat caaccgtctc tctatctgtc ataatcacac cagccttttc
     3241 caatttaggc cggccatttg gaaaactatc ttcaataact aaataatgaa ctacctcagt
     3301 attagtgaaa gcagcaatat tggtatgttt ttttgtgtga atgatatcta aatcctcgag
     3361 ccccatcatc aatagatctt cctttacatt ctctgaagga tttggcgtaa ttcgatcaat
     3421 catactccaa ggaaaagtca ctttttcttt atcctttaaa taagcaataa atttttcaga
     3481 aacatatcct tctttttgcc aagcttctgc aatcgtaaaa atactatctt gaaaacgttg
     3541 accattctgt gagaaattat cagtagaaac catcgcaatc ggcaactctc cagcattaaa
     3601 tctgctatat aacaaggatg ttacaatact catcgtatgt tgaggattgc taggtccaga
     3661 tttgatatca gctttgacta ccgaaaagaa ttgcccttta gaatctttta aactgtaacc
     3721 tttttcagta atagtaaacg ttgtaaactg taatttagga tcttcaaaat atttaaccac
     3781 tctttgatag tccgctatat tcttcggatt gcaatataaa ctatcagccg ttactgccaa
     3841 aagtcttttt ttgagttcac catctccact cataacaatt tccaaaatgt cattgttata
     3901 gtcatgataa gctttttcca ctacttcttc atcaaatgtt tcacacacaa ctactcctgt
     3961 ttctaattca ttttgatctg ctaagcattg agcaatttct gcatgaaatc ctcgataaag
     4021 atttcctcca ccaaaatgaa gccatactgg atgttgctgg ccttcttgct taacttcttt
     4081 gacatcaaaa cacggcagta gaatctgcat ttcattgaat tcttcgatag aattttgcca
     4141 atttttaatc gaaaccataa ttaacctcct caaactgata tgttagtttt gatacatttt
     4201 atattttaaa acccattgta accgttgtca atataaaaag ggaataaact tgcttttttc
     4261 taccatatca gaatgattgt attttttatt cagaaaaata aaatactcct tctttttgga
     4321 ctgattttat cgaaacttat tttttctttt tttcatgtat tcttttattt ttataactag
     4381 tgtattactt caaaatttga tattatatgg catgaacaga attaaaatat cagttaaaat
     4441 aaaagttaag gagcattgtg tatgacaatt aaaactagtc tccaaaatca agcttaccaa
     4501 tctattcgcc gacaaattat ctatgctgac ttagaacctg gaaaaaaagt atcagaaaaa
     4561 aaattagaag aaacactttc tattggtcgt acaccaatac gtgaagcact gattcaactc
     4621 cgacaacaag gtcttgttga tacaattcct caaagcggaa attatatctc attaattgat
     4681 ttagattcgg ccaaaaatgc tcgatttgtc agagaacatt tggaacgaca aattatgtta
     4741 gagtgttgtg ccaaaatgaa taaccaaaaa aaacggattt tagaaactat aatttcagaa
     4801 caagaaaaag ctgttagtca aaaaaatgct cgtagttttt ttcataatga taatttattc
     4861 cataaagctt gttttgaaat tgcagagcga gaagaagtat ggcaatggtt agaaaataac
     4921 aatacacact tagaacgatt tcgatggcta cgagtaaaaa cggaaggatt aaagtgggaa
     4981 aaaattatgg aacaacatta tcaactattt gaggcattaa caaaacaaga tccagaagaa
     5041 gctaactttc taacatcgtt gcatcttcat ttaatgctta atgagcaaaa tattgtttta
     5101 gctactcatc caaaatattt caaaaatata taacttttaa ctctttttaa attaaatggt
     5161 tgt
//
LOCUS       DAZKOW010000139         5137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000139 DAZKOW010000000
VERSION     DAZKOW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.143"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..447)
                     /locus_tag="ACG912_002684"
     CDS             complement(<1..447)
                     /locus_tag="ACG912_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0513949.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
     gene            complement(754..1851)
                     /locus_tag="ACG912_002685"
     CDS             complement(754..1851)
                     /locus_tag="ACG912_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFQ0513950.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1959..3986)
                     /locus_tag="ACG912_002686"
     CDS             complement(1959..3986)
                     /locus_tag="ACG912_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFQ0513951.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4131..4859)
                     /locus_tag="ACG912_002687"
     CDS             complement(4131..4859)
                     /locus_tag="ACG912_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFQ0513952.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1456 a         1040 c          813 g         1828 t
ORIGIN      
        1 caactttctc ttttcaaaaa aaatttgata tttttttaga tttcttttta gtctttgtaa
       61 tactttataa gccacagaat aactcacgtg atgctgttta gcaaaatttt ctactgagat
      121 atatttttcc tgaaagaaag ccaataccat tttcatagat aaagaatccg ttatataacg
      181 agataacaga gctttgctag aacattttcc attaatttcc agacatatat ccaattcact
      241 gacggttact cgcaaatttt tctctagatc gaacttttgt aaatcatgtt tcagcttatc
      301 gatcgttttt ttcaagagaa atgtcgatag cgacaactct tcttgaacat tcgatattga
      361 ctctgataaa tttgagcttg cttctaaata tttaagtaat gaataacttc tgtgatcttc
      421 tttgcttaaa aaaaactcat aattcattag tcatcagctc tttctacgta tattactttc
      481 catgcattca ccaactagct ggggataaaa ctgatttcct ggatccgagt cactgttgtc
      541 agcgattctt gtttgtataa tatacatcgt cctgtctttt ttttaacatt ttggagagcg
      601 ttttatttgg cctttctcgc gatcaaacct gttattagaa ttatatcatt tactagtatg
      661 catttttatt aataaaatgg tatattttat attgttataa aaagagccca accatcctac
      721 aataataaaa atttactatt tgttatctta accttacttt tctttctctt ctttttttct
      781 caaaacaaac aaaagtatgc ataataaaat aaacaataag aaaagtgaag ctactaagat
      841 ataaatccaa ttattgtctt ttttaatcgt aacatccgtc gtgttcaatt ccttggctac
      901 ttttccgtct attgtaaaat ctttttcaaa tacccattga ttggtatagt tgatcgtagt
      961 atttcctttt tttctcgaaa attttccatt ttcattttca tttcctaaaa cagtcattgt
     1021 aaggtgatat tcacctgggg ttagcggttg accatttaaa gcagttggaa aagaaaaatt
     1081 tgtattcggc gcaatctgca atccttcttt ttcctcttga tataaaactt cagaatgtcc
     1141 tttttttgta atttttgttt caataactac ttgattgata taggtttttt gatcattttg
     1201 aacgtttgct aagatgacat ttcttgcatt gatttgatct ggtttaactt catgaagagt
     1261 aaggttcgga gcaacttcgt tcagtgtctg ttgcatcaac aaagcaataa tataggtgta
     1321 ttcattctca atcgataagc tttgatcctt agtttctgtt tggtcctttt ccgtctggat
     1381 ttcttggaat gtgatcccac cagccaatac accatcaaat cgctcattcg gcatcctgac
     1441 atgaaaagca acggtttgga cacttttagg ttttagcgtc actatctgtg gatagctcac
     1501 ataatctacc agatcaaaga tcaggctcat gtctttttca atctcgtttt ttccgtattc
     1561 aaccatcaca tttgaattgg tggttgcgct attcacagaa atatcgattt tcacttcttt
     1621 ttctgtatca tttctcaatt ccgcttttac ttctacttcc tgatccggat caagttgaag
     1681 atcgaagtaa gtttttctct tgtcgatttg attctctgaa gttatcggcg tcactgaaaa
     1741 attaaatgca ctagctgaca ctgatatatt gttaaaaagt gcccctaaaa taactaggag
     1801 tgttataaat aattgttgtt ttggtctcaa gaaaatcgct cctttaatca tatgtaagag
     1861 aagactataa caaaatcttg tctctgtggg acacaagagc ctatggtacc tgtgcccttc
     1921 ttgaaactca ataattttgt tatattcttc tcttcctgtt aatttcctgg gacatctgca
     1981 agtatccaag atagtttccc ctcaaaggtt ccgattcttt ggttttctac aggaatattt
     2041 attctgattc cacgatgagc tgtagaatct aaaagagagg acaaatcgct ttcacccttt
     2101 tgatcacttt cgaaaatcgt aacagcggat gtccctataa ccgttgtatt ttcagaggta
     2161 acaaaggaaa gagcttctgg aagctcaaaa tctccatttg taagaacttt ctcttgtttc
     2221 aattgtaatt tccaacttcc aaaagcccgt tcatctataa ttttcaaccc acctgaaaac
     2281 tgtccgaatt cacttaatgg tctactggat attttatgtt ttccaaaatt gacatattga
     2341 ggagcagttg agagataaag tttgcctttt ctgtaataaa aatagatatc ctctgtattt
     2401 tcggagaaga tgccttctgt attcgctgaa tccgagtcaa aaacataccc ttcaatatca
     2461 ataggagttt ctttatactt ctctcctatt ttcccagtga ttgttttcat ttcagcaata
     2521 tcaatctgac tgtctttatc tttataataa acatggatat ctatctcctt cagctttgcg
     2581 atctttaggt ttatttcatt cgattgctca atcaaattcc cgttttcaag gtattcaaaa
     2641 tgcatagtga gtactttttc aggaattact tcctgcaaag ttgtctccaa ttcgattatt
     2701 aacgtatctc cagcaataaa ataatctgtc agatttagcg tgactcgatt tccggttatc
     2761 aagatatctt tggcatcaat ttgatttcca ttgatcaaga tatgctcggg aatagttaat
     2821 tcttgtggga ggtcgatact agtctgcatt ttcttatatt caagaaaatc cccctcgacc
     2881 ttcaatgtat ttctgatagt tatcttatcg tttcctccag cttcagcgat ggccccataa
     2941 tcactatcgt tgacttttac atcagctgtt gatgtgacat tcgatggaag tcgcgtgaca
     3001 gcgacagcat tttcttgaaa aaaagtacct gttgaagaag taaaccccca atttacttct
     3061 gtagaaccaa actgcctaat gagatcaggt ataatccgag aagagcgacc gacaccttcc
     3121 aaatcataag taagacttct ttgctccgcg tcccatttaa ttattaacaa gcgccatgtg
     3181 ccattagaaa gaaatgtagg agctaccgtc acgccagtat gctctccaga ataattatta
     3241 ttatttggtt taggcgtgac aaaagcgaca tgtccatgcc ctctattatc agcagttatc
     3301 tctcgatcca ttcgatcaga agaaccacta tttttatacg tatcaaactc aatcgagagc
     3361 gcattcgcaa tgtaaggttg gccagaacga gtagaatatg agccaattcc catacctggc
     3421 gatccaataa cttgactggg attactagcc attctaggat cgccagtcaa tgtaaaggtc
     3481 atgccatctc ccgctctatc tcgttgattt cctaagtata aataagattt aaatgtaaaa
     3541 tccgagagta aactaatctt cttatttgac caaatagctc ctctttgaga cctttctgct
     3601 gggttcaact gcagtaactt cccattttcc aatatggacg tgctagtacc gataggtgtt
     3661 tgaaaaattt tatcgataga aataatattg ggatcgtcag gcagctcaac cgcagaaatt
     3721 ggtgcatctt ctagtccttg tttgtccgaa ccctcagctt ctacgttgtc agtggtctct
     3781 ccactactcg aaatacttaa atcctcactg ctttcttgtg tctggttgga ttctattaaa
     3841 ctgtcgaatg tcgaagtgag tttgctttct tcgtctcttc tatttccatg ctccaactga
     3901 ttcaattcca aagagtaccc tatgatcgga aagatattag tcactaagag actgcttaaa
     3961 atgagcacga tcctcctctt tttcaatata tcttttcctc ctgatcgtta aattcctaga
     4021 tgaactattc gtatccaaac aaatttagcc tagcctttga caatttttgc tcgattacct
     4081 cgcaagacaa tatgactacg tatttatttt taacagttct tttcagtaaa ctagttgtta
     4141 ggtgtatctt ctaatgtcca aactaattta gtggaatatt gttgcgctaa cttgacagat
     4201 ttaccgggta ccgaaagctg taccgcatct tgattttcat tttcatttgc accaaatcga
     4261 tagacccaag tccccacacc tgttcctgat ttagcattaa ttgctaacgt attgtttcct
     4321 cccggtgtca acgttaattc agaattcacc gtttgcggcg tatatgttgg atcaaccaaa
     4381 gaaacagcct gccctttcgt aaatttcagc tgtgctccaa ctagttcatc cccactggca
     4441 gttttaaact gttctggttg agagacactt aatttccacc cagataaggt acttctccta
     4501 tctgtcactt ggacatatgt cggttttcta gacccatcgt tcatcacatc agcagctgca
     4561 taatacgttt tatccgcagt agaaattttt tgagtcccaa atttgaattt tgatccataa
     4621 tcaatcgata atgcccctcc tgttccaggg cttggaggat ctgatggatc gacaggttta
     4681 ttgggatcag gattctctgg atcaacagga ggagttacac cctcatctcc tgcttcaaat
     4741 gtaatcgttc cttctgtgtt atattccttt gggtatactt cagttgcaga aacaaaagtc
     4801 gtacttgcta ccatgctgaa taacatcatt gctgctgtta atacgatttc ttttttcatc
     4861 attgacattc ctctttcttt atttactaaa catatcagtc gattggatta atccatagca
     4921 aaattctgtt ttacgatact tatgaaataa aagaacatag acctatattt ggtcctatgt
     4981 atcgattagt tataaatgat tatcagcccg atttgatttc tagtttttat tgcaacttga
     5041 tgcaaacaca atctagaaat acagctgtca tagcctttaa aataattcgt aagtccccct
     5101 cctatctatc aaaatgtgac ttgacgagag tgtaaaa
//
LOCUS       DAZKOW010000140         5063 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000140 DAZKOW010000000
VERSION     DAZKOW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.144"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..930
                     /locus_tag="ACG912_002688"
     CDS             <1..930
                     /locus_tag="ACG912_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFQ0513953.1"
                     /translation="DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDV
                     STAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEG
                     IKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKP
                     QVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVT
                     ATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKT
                     GESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1064..2407
                     /locus_tag="ACG912_002689"
     CDS             1064..2407
                     /locus_tag="ACG912_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFQ0513954.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            2519..3922
                     /locus_tag="ACG912_002690"
     CDS             2519..3922
                     /locus_tag="ACG912_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0513955.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            4271..4903
                     /locus_tag="ACG912_002691"
     CDS             4271..4903
                     /locus_tag="ACG912_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFQ0513956.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         1959 a          680 c          965 g         1459 t
ORIGIN      
        1 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
       61 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      121 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      181 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      241 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      301 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      361 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      421 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      481 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      541 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
      601 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
      661 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
      721 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
      781 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
      841 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
      901 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
      961 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1021 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1081 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1141 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1201 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1261 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1321 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1381 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1441 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1501 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1561 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     1621 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     1681 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     1741 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     1801 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     1861 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     1921 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     1981 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2041 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2101 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2161 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2221 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2281 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2341 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2401 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2461 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2521 gaaaaaatct ttaaaacttg catcagcagt tatattaatg tcccaactcc ttatagcaac
     2581 acctttccat gtgttggcaa gcgaaaattc aacatacaaa attgcaaaag aaagaaccga
     2641 gacgactgaa atgagtaatt ccgatgtaac agcagagagt agtgaacttt tgacaacttc
     2701 atcaagtgaa agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag
     2761 tagtacgact tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc
     2821 aagtaccgaa tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac
     2881 aaaaccaaac catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa
     2941 aactttttcg acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact
     3001 atctggtaat gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa
     3061 aattggtgag tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat
     3121 tgcacaagcg atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta
     3181 caatttattt gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga
     3241 agatttgggt aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta
     3301 tgaggaaagc tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga
     3361 tttttatgca ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct
     3421 tacaggacgg tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac
     3481 ctacgattta accaactatg ataaagaagt taaaggtcca caaatgagta agactggata
     3541 ccaagtgcct attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt
     3601 tcaccgagga cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg
     3661 taaggttgtt aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga
     3721 agatggatca accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa
     3781 tgttttgcaa ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca
     3841 ccttcatttg gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa
     3901 aaccttatta ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt
     3961 tggattctta gacctttgat caggatattg agactggaat tgatatctcg attacgatgg
     4021 gtttgacaaa taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc
     4081 tcatattttg agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt
     4141 acgtatcgtt cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa
     4201 atattttttt ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag
     4261 ggtaggtgca gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa
     4321 tggatataaa aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc
     4381 aaacttacta tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa
     4441 atataaaaat ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact
     4501 gcgaagtgaa ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt
     4561 atcggcaaag cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa
     4621 gcatcacatt gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa
     4681 ctgttcatgg aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag
     4741 ttatagtaac ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac
     4801 aaggacatgt gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa
     4861 tacaagtggt gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt
     4921 aaaaaaaaag aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag
     4981 aaaagatgct gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta
     5041 attattgtgg ataataatta aca
//
LOCUS       DAZKOW010000141         4933 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000141 DAZKOW010000000
VERSION     DAZKOW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.145"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACG912_002692"
     CDS             85..972
                     /locus_tag="ACG912_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFQ0513957.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACG912_002693"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACG912_002693"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFQ0513958.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACG912_002694"
     CDS             2274..2720
                     /locus_tag="ACG912_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFQ0513959.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACG912_002695"
     CDS             2993..4345
                     /locus_tag="ACG912_002695"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFQ0513960.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACG912_002696"
     CDS             4516..4803
                     /locus_tag="ACG912_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0513961.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAZKOW010000142         4892 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000142 DAZKOW010000000
VERSION     DAZKOW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.146"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACG912_002697"
     CDS             complement(287..649)
                     /locus_tag="ACG912_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513962.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACG912_002698"
     CDS             complement(723..1103)
                     /locus_tag="ACG912_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFQ0513963.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACG912_002699"
     CDS             1185..2360
                     /locus_tag="ACG912_002699"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFQ0513964.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACG912_002700"
     CDS             complement(2437..3021)
                     /locus_tag="ACG912_002700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFQ0513965.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="ACG912_002701"
     CDS             3222..4220
                     /locus_tag="ACG912_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFQ0513966.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="ACG912_002702"
     CDS             4332..4841
                     /locus_tag="ACG912_002702"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFQ0513967.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
BASE COUNT         1553 a          914 c          851 g         1574 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt tt
//
LOCUS       DAZKOW010000143         4757 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000143 DAZKOW010000000
VERSION     DAZKOW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.147"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            51..746
                     /gene="vanR-A"
                     /locus_tag="ACG912_002703"
     CDS             51..746
                     /gene="vanR-A"
                     /locus_tag="ACG912_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0513968.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            724..1878
                     /locus_tag="ACG912_002704"
     CDS             724..1878
                     /locus_tag="ACG912_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0513969.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2093..3061
                     /locus_tag="ACG912_002705"
     CDS             2093..3061
                     /locus_tag="ACG912_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0513970.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3054..4085
                     /gene="vanA"
                     /locus_tag="ACG912_002706"
     CDS             3054..4085
                     /gene="vanA"
                     /locus_tag="ACG912_002706"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0513971.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4091..4699
                     /locus_tag="ACG912_002707"
     CDS             4091..4699
                     /locus_tag="ACG912_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0513972.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1471 a          910 c         1115 g         1261 t
ORIGIN      
        1 tataatctga taaattatta agggcaaact tatgtgaaag ggtgataact atgagcgata
       61 aaatacttat tgtggatgat gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa
      121 acgagaatta tacggttttc aaatactata ccgccaaaga agcattggaa tgtatagaca
      181 agtctgagat tgaccttgcc atattggaca tcatgcttcc cggcacaagc ggccttacta
      241 tctgtcaaaa aataagggac aagcacacct atccgattat catgctgacc gggaaagata
      301 cagaggtaga taaaattaca gggttaacaa tcggcgcgga tgattatata acgaagccct
      361 ttcgcccact ggagttaatt gctcgggtaa aggcccagtt gcgccgatac aaaaaattca
      421 gtggagtaaa ggagcagaac gaaaatgtta tcgtccactc cggccttgtc attaatgtta
      481 acacccatga gtgttatctg aacgagaagc agttatccct tactcccacc gagttttcaa
      541 tactgcgaat cctctgtgaa aacaagggga atgtggttag ctccgagctg ctatttcatg
      601 agatatgggg cgacgaatat ttcagcaaga gcaacaacac catcaccgtg catatccggc
      661 atttgcgcga aaaaatgaac gacaccattg ataatccgaa atatataaaa acggtatggg
      721 gggttggtta taaaattgaa aaataaaaaa aacgactatt ccaaactaga acgaaaactt
      781 tacatgtata tcgttgcaat tgttgtggta gcaattgtat tcgtgttgta tattcgttca
      841 atgatccgag ggaaacttgg ggattggatc ttaagtattt tggaaaacaa atatgactta
      901 aatcacctgg acgcgatgaa attatatcaa tattccatac ggaacaatat agatatcttt
      961 atttatgtgg cgattgtcat tagtattctt attctatgtc gcgtcatgct ttcaaaattc
     1021 gcaaaatact ttgacgagat aaataccggc attgatgtac ttattcagaa cgaagataaa
     1081 caaattgagc tttctgcgga aatggatgtt atggaacaaa agctcaacac attaaaacgg
     1141 actctggaaa agcgagagca ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt
     1201 atgtacttgg cgcacgatat taaaacgccc cttacatcca ttatcggtta tttgagcctg
     1261 cttgacgagg ctccagacat gccggtagat caaaaggcaa agtatgtgca tatcacgttg
     1321 gacaaagcgt atcgactcga acagctaatc gacgagtttt ttgagattac acggtataac
     1381 ctacaaacga taacgctaac aaaaacgcac atagacctat actatatgct ggtgcagatg
     1441 accgatgaat tttatcctca gctttccgca catggaaaac aggcggttat tcacgccccc
     1501 gaggatctga ccgtgtccgg cgaccctgat aaactcgcga gagtctttaa caacattttg
     1561 aaaaacgccg ctgcatacag tgaggataac agcatcattg acattaccgc gggcctctcc
     1621 ggggatgtgg tgtcaatcga attcaagaac actggaagca tcccaaaaga taagctagct
     1681 gccatatttg aaaagttcta taggctggac aatgctcgtt cttccgatac gggtggcgcg
     1741 ggacttggat tggcgattgc aaaagaaatt attgttcagc atggagggca gatttacgcg
     1801 gaaagcaatg ataactatac gacgtttagg gtagagcttc cagcgatgcc agacttggtt
     1861 gataaaagga ggtcctaaga gatgtatata attttttagg aaaatctcaa ggttatcttt
     1921 actttttctt aggaaattaa caatttaata ttaagaaacg gctcgttctt acacggtaga
     1981 cttaataccg taagaacgag ccgttttcgt tcttcagaga aagatttgac aagattacca
     2041 ttggcatccc cgttttattt ggtgcctttc acagaaaggg ttggtcttaa ttatgaataa
     2101 catcggcatt actgtttatg gatgtgagca ggatgaggca gatgcattcc atgctctttc
     2161 gcctcgcttt ggcgttatgg caacgataat taacgccaac gtgtcggaat ccaacgccaa
     2221 atccgcgcct ttcaatcaat gtatcagtgt gggacataaa tcagagattt ccgcctctat
     2281 tcttcttgcg ctgaagagag ccggtgtgaa atatatttct acccgaagca tcggctgcaa
     2341 tcatatagat acaactgctg ctaagagaat gggcatcact gtcgacaatg tggcgtactc
     2401 gccggatagc gttgccgatt atactatgat gctaattctt atggcagtac gcaacgtaaa
     2461 atcgattgtg cgctctgtgg aaaaacatga tttcaggttg gacagcgacc gtggcaaggt
     2521 actcagcgac atgacagttg gtgtggtggg aacgggccag ataggcaaag cggttattga
     2581 gcggctgcga ggatttggat gtaaagtgtt ggcttatagt cgcagccgaa gtatagaggt
     2641 aaactatgta ccgtttgatg agttgctgca aaatagcgat atcgttacgc ttcatgtgcc
     2701 gctcaatacg gatacgcact atattatcag ccacgaacaa atacagagaa tgaagcaagg
     2761 agcatttctt atcaatactg ggcgcggtcc acttgtagat acctatgagt tggttaaagc
     2821 attagaaaac gggaaactgg gcggtgccgc attggatgta ttggaaggag aggaagagtt
     2881 tttctactct gattgcaccc aaaaaccaat tgataatcaa tttttactta aacttcaaag
     2941 aatgcctaac gtgataatca caccgcatac ggcctattat accgagcaag cgttgcgtga
     3001 taccgttgaa aaaaccatta aaaactgttt ggattttgaa aggagacagg agcatgaata
     3061 gaataaaagt tgcaatactg tttgggggtt gctcagagga gcatgacgta tcggtaaaat
     3121 ctgcaataga gatagccgct aacattaata aagaaaaata cgagccgtta tacattggaa
     3181 ttacgaaatc tggtgtatgg aaaatgtgcg aaaaaccttg cgcggaatgg gaaaacgaca
     3241 attgctattc agctgtactc tcgccggata aaaaaatgca cggattactt gttaaaaaga
     3301 accatgaata tgaaatcaac catgttgatg tagcattttc agctttgcat ggcaagtcag
     3361 gtgaagatgg atccatacaa ggtctgtttg aattgtccgg tatccctttt gtaggctgcg
     3421 atattcaaag ctcagcaatt tgtatggaca aatcgttgac atacatcgtt gcgaaaaatg
     3481 ctgggatagc tactcccgcc ttttgggtta ttaataaaga tgataggccg gtggcagcta
     3541 cgtttaccta tcctgttttt gttaagccgg cgcgttcagg ctcatccttc ggtgtgaaaa
     3601 aagtcaatag cgcggacgaa ttggactacg caattgaatc ggcaagacaa tatgacagca
     3661 aaatcttaat tgagcaggct gtttcgggct gtgaggtcgg ttgtgcggta ttgggaaaca
     3721 gtgccgcgtt agttgttggc gaggtggacc aaatcaggct gcagtacgga atctttcgta
     3781 ttcatcagga agtcgagccg gaaaaaggct ctgaaaacgc agttataacc gttcccgcag
     3841 acctttcagc agaggagcga ggacggatac aggaaacggc aaaaaaaata tataaagcgc
     3901 tcggctgtag aggtctagcc cgtgtggata tgtttttaca agataacggc cgcattgtac
     3961 tgaacgaagt caatactctg cccggtttca cgtcatacag tcgttatccc cgtatgatgg
     4021 ccgctgcagg tattgcactt cccgaactga ttgaccgctt gatcgtatta gcgttaaagg
     4081 ggtgataagc atggaaatag gatttacttt tttagatgaa atagtacacg gtgttcgttg
     4141 ggacgctaaa tatgccactt gggataattt caccggaaaa ccggttgacg gttatgaagt
     4201 aaatcgcatt gtagggacat acgagttggc tgaatcgctt ttgaaggcaa aagaactggc
     4261 tgctacccaa gggtacggat tgcttctatg ggacggttac cgtcctaagc gtgctgtaaa
     4321 ctgttttatg caatgggctg cacagccgga aaataacctg acaaaggaaa gttattatcc
     4381 caatattgac cgaactgaga tgatttcaaa aggatacgtg gcttcaaaat caagccatag
     4441 ccgcggcagt gccattgatc ttacgcttta tcgattagac acgggtgagc ttgtaccaat
     4501 ggggagccga tttgatttta tggatgaacg ctctcatcat gcggcaaatg gaatatcatg
     4561 caatgaagcg caaaatcgca gacgtttgcg ctccatcatg gaaaacagtg ggtttgaagc
     4621 atatagcctc gaatggtggc actatgtatt aagagacgaa ccatacccca atagctattt
     4681 tgatttcccc gttaaataaa cttttaaccg ttgcacggac aaacggttct gttgcaaagt
     4741 tttaaataaa gaataaa
//
LOCUS       DAZKOW010000144         4434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000144 DAZKOW010000000
VERSION     DAZKOW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.148"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACG912_002708"
     CDS             complement(543..2048)
                     /locus_tag="ACG912_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0513973.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACG912_002709"
     CDS             complement(2045..2731)
                     /locus_tag="ACG912_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFQ0513974.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG912_002710"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACG912_002710"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFQ0513975.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAZKOW010000145         4425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000145 DAZKOW010000000
VERSION     DAZKOW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.149"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            690..1658
                     /locus_tag="ACG912_002711"
     CDS             690..1658
                     /locus_tag="ACG912_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0513976.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1726..2484
                     /locus_tag="ACG912_002712"
     CDS             1726..2484
                     /locus_tag="ACG912_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFQ0513977.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGDSFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="ACG912_002713"
     CDS             2552..3262
                     /locus_tag="ACG912_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFQ0513978.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="ACG912_002714"
     CDS             3298..>4425
                     /locus_tag="ACG912_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0513979.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DAZKOW010000146         4377 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000146 DAZKOW010000000
VERSION     DAZKOW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.150"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..234
                     /locus_tag="ACG912_002715"
     CDS             73..234
                     /locus_tag="ACG912_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFQ0513980.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACG912_002716"
     CDS             253..1530
                     /locus_tag="ACG912_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513981.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACG912_002717"
     CDS             1540..2196
                     /locus_tag="ACG912_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0513982.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACG912_002718"
     CDS             2196..3572
                     /locus_tag="ACG912_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0513983.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..4115
                     /locus_tag="ACG912_002719"
     CDS             3702..4115
                     /locus_tag="ACG912_002719"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0513984.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIADCRQKLFQTRNSSLEQLFIYFVSNF"
     gene            complement(4140..4377)
                     /locus_tag="ACG912_002720"
                     /pseudo
     CDS             complement(4140..4377)
                     /locus_tag="ACG912_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1699 a          522 c          761 g         1395 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAZKOW010000147         4330 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000147 DAZKOW010000000
VERSION     DAZKOW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.151"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..567
                     /locus_tag="ACG912_002721"
     CDS             130..567
                     /locus_tag="ACG912_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0513985.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWVFYLVKK"
     gene            662..1546
                     /locus_tag="ACG912_002722"
     CDS             662..1546
                     /locus_tag="ACG912_002722"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFQ0513986.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPEAINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSILLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1768..>4330
                     /locus_tag="ACG912_002723"
     CDS             1768..>4330
                     /locus_tag="ACG912_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFQ0513987.1"
                     /translation="MNQYVKKLIASILFVFPLLMVTSVFADVEKTSESTQSENQSFDK
                     TTFSEESITSETTESQSEAPTSNTRQEENKTSETSEVAEQTSDSSEEVQVRVVSADGY
                     SEAATTEELTQLLADESVAKIRLTQPLTLDRELEIKRDIVIDFGGLAHNFGTHHIYIN
                     ETPQSIEFQNFKGTAAHPGGLIPNIDGNAIIIAYMSDFWGNRYHFTGEIKFTGTLDLY
                     DGSKLGLIYAPRATVTLDGVSGVLDVQPVKEGMNAAPGKAYFCRSYQLNVINGSQLYG
                     PYLGKFYGFLDSGDETGSGKTAPGLHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVT
                     FKVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSRVAVSNDGKITINTATTGGFRLQG
                     DSSQIHVSSGGQISVKMAGDGDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSA
                     VRFENGTQKLTVTNGGSLKVRNAGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFT
                     VTGEKSNIDIRADSGATVDTTGDIDLNFMAGEKTYLVMVGKTGEDKTGIISGDVLNVD
                     LESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKAGVTFDNFEKDP
                     DYFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRV
                     PTNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSPVEITALTQGQTDE
                     NEGTGYNPWGEGEQGGLFEVQVPNEERLTTGDQVQIVAAKKVRGGQEVTELDSAKTTV
                     DVIPPEPAKLSTTLINAASRSITGTGEAGALVSLKKNQAVLSETTIDDTGKFVLSI"
BASE COUNT         1414 a          724 c          965 g         1227 t
ORIGIN      
        1 attattactt gatttagttc tttttatttt aaaaattttt tttacttaga atccagccac
       61 cctagatatt tttctgtata atcttaaata aagaaaacga tattaaaaaa tgcacgtaga
      121 agtagataaa tggagtggtt ttgcaaattt cttttatttc tagaagttat tcacgtatac
      181 ggatttgttt ttctcacaaa taaaaaggca ggaagtacaa tggatcattt gtatataaaa
      241 tatattcatc aattttccgt tttggtatta ggcacactaa ttatagcggg gattgttgca
      301 tttattattc aaggtcgtga catattaaca gtacatcaag agtttttcag ttatgcattt
      361 ggtattcctc tgatcgctat aaggtctgat aacaaaaagt ggaagaaacg ctattacatt
      421 ctcttgctat ttttgtgttt ttatctgcca cctaagattt ttactttatt aggtatacaa
      481 tgcctatcat tttcttttat ttcctatatc attgttttaa ttttcctcag tttcatagta
      541 tgggtatttt atcttgtcaa aaaataaatg aatgtttcat gaacagtact tcatcacagc
      601 ctaatttttt tctttatgct atcgttatat taaagaaatc aatcagaagg gaaagaaagt
      661 gatgaaaaaa gttttattag tggtaaaccc cagttcaggc ggtgaacaag caaaggaatt
      721 tgagcaatta gcgatagcaa aattggaatc ggtgtttgat gaagtggtcg tcttgcatac
      781 aaaaaaagca ggggatgcaa aaaactttac tcgcgaagcg gctacggaag ggtatcatag
      841 tgtgtttgtc atgggcggag atggaacagt caacgaagga atcagtggaa tcgctgaaca
      901 agaacatcgg ccaaatttcg gatttttccc attgggtacg gtgaatgact tggctcgagc
      961 attagggata ccgctagaac ctgaggaagc gatcaatcat ttttcgattg aatcagtcaa
     1021 gccattggat attggaaaaa taaatgacga ctattttatg aatgtcgtcg caattggctc
     1081 tatccctgaa gcaatcaatg atgtagatgc ggagaagaag acgaaatttg gaaaactagc
     1141 ttatttcatg tcaggaatca aacagttagc ttccacacaa tcctactcgt ttcacgtaga
     1201 agttgatgga aagaaggaag aaattgagag cagtatcttg ctgataggac taactaactc
     1261 agtaggcggt tttgaaacat tacttccaaa tgcaaaagtg gatgatggta agctccattt
     1321 ggtttattta aaagattctt ctttgatcga tacgcttaaa acattgccag acctattaaa
     1381 aggagtagat gaatctacga aaaatctagc ctatcttact tgtgagcaat tagttgtgtc
     1441 attagctgat gaggaagaat tgacgacgaa tgttgatgga gatgaagggg ataaattacc
     1501 tgtgacaatc aaagtgttac cctcacattt acaggtttat tgttaaaatg gaggttgtga
     1561 ccgaagagtt gtcaggagga catcggttgt tcggaataag ggactgtgac aagacttttg
     1621 tcacagcctt tttttgtaca aaaaagaaat tttcacctaa cagaaaaatt tgccataaaa
     1681 atacatattt taaatatgaa agtacaatta caaataaatt aaaacgttaa aataaaattg
     1741 tcaaaacgtc agagaggatg gtggagaatg aatcaatatg taaaaaagct gattgcgtcg
     1801 attttgtttg tcttccctct cttgatggtg acatccgttt ttgccgatgt agagaaaaca
     1861 agcgaatcaa cgcaatcaga gaatcaaagt tttgacaaga ctactttttc agaagaatca
     1921 attacttctg aaactacaga atcccaaagt gaggcgccta cttctaatac aaggcaagaa
     1981 gaaaataaga caagtgagac ctcagaagta gctgaacaaa cgtctgatag cagtgaagag
     2041 gtacaggtta gagttgtttc agcggatggg tacagcgaag ctgccacaac agaagaactg
     2101 acgcagcttc tcgcagacga atcagtagct aagattcgtt taactcagcc tttgacttta
     2161 gacagggaat tggagatcaa gcgagatatc gttatcgatt ttggcggact tgctcataat
     2221 ttcggcacac atcacatcta tatcaatgag acacctcagt cgatcgagtt tcagaatttt
     2281 aaaggaacgg cagctcatcc aggtgggttg ataccaaata tcgatggaaa tgccattatc
     2341 attgcttata tgtccgattt ttggggaaac agatatcatt ttacgggaga aataaaattt
     2401 accgggacac ttgacttata tgatggttct aaattaggcc tgatttacgc ccctcgtgca
     2461 actgtcacat tagatggtgt ttccggtgta ttggatgtcc aaccagtgaa agaagggatg
     2521 aatgccgctc cgggtaaagc ttatttttgt agaagctatc agctaaatgt aatcaatggt
     2581 agtcagcttt atggaccata tctaggaaaa ttctatggtt ttctagattc aggtgatgag
     2641 acgggcagtg gaaaaacagc accaggactc catatcttga gtggttcaaa ggtctctctt
     2701 gactatgatc gggcagatgg cgtcagcgat gaaggagagg ctgtcgatac gctacccggg
     2761 aatgtcacat ttaaagtcag tggctcagga agtgaatttt cggtaaatac aaagatcaat
     2821 attaccaacg acaacaatcg cggaattatc caaatgagag gctcaggatc aagagtcgct
     2881 gtgtcgaatg atgggaaaat cacaatcaat acagcgacta ctggcggatt tcgcctgcaa
     2941 ggagattcta gtcaaatcca tgtatcaagt ggtggacaaa tctcggtcaa aatggctgga
     3001 gatggcgacc aacctggaaa caatgggatg cgttttgtag ggaaaggact ttctttgatc
     3061 gtagaaggag ccggatcagt catcgatatt gataagcaaa gtggtcgttc tagcgcagtt
     3121 cgttttgaga acggtacgca aaaactaaca gtaactaatg gaggctcatt gaaggtaaga
     3181 aatgcagggg atggcaaatc ttatgtgaac agactcaatc aaggaaacca agcgatccaa
     3241 ttttatgacg cttctggttt tctagaaagt ccagggtcag ctgattttac ggttactgga
     3301 gaaaaatcaa atatcgatat acgcgcagat tccggagcta ccgtagatac aactggagat
     3361 attgatttga actttatggc tggcgaaaaa acttatttag tcatggtcgg gaaaactggg
     3421 gaagataaga ctggcatcat cagtggggat gtgttgaatg tagacttaga atctcctacc
     3481 tatttcgatt tccaaaacaa acggatcgga agcgagacta atggcatagg tggctgggta
     3541 tttcaaggaa atggcgattc ttctttgaag atcgctcatt cagaaattgc tttatggcga
     3601 aaggcaggag taacgttcga taactttgaa aaagatccgg attatttttc tgatatgaca
     3661 gacttatcga tcaatggaac cgacttaggt aattttgtat caagccctag tgcaggttta
     3721 acaaatgaat ttgctcagac aggtgccgga atgaaaaatt ataatcgaat cagtgccaat
     3781 aaccagcttc cgatgattga atccttaaga gtaccaacga atgcagataa aaaaatctat
     3841 gggcatgtcg ttgttcccga aggtgtcgag gcaaagccaa gagatgcttg gacaggcgaa
     3901 gtagaagtga ctttacgtat tacttatgca gatacgacga aatcacctgt agaaattact
     3961 gctctcacac aaggacaaac agatgaaaat gaagggacag gatataatcc gtggggagaa
     4021 ggcgaacaag gcggtttgtt tgaggttcaa gtacccaacg aagaaagact aacaacgggg
     4081 gatcaagtgc aaatagttgc tgctaaaaaa gtaagaggcg gccaagaagt gacagaactg
     4141 gactctgcca aaacgacagt cgatgtgatt cctcctgagc ctgctaaatt aagtaccaca
     4201 ttgatcaatg cagcttctcg ctcaatcaca ggtacaggcg aagcaggagc tttggtttca
     4261 ttgaaaaaaa atcaagctgt tttgtccgaa acaactattg atgatacagg taaatttgtt
     4321 ctttctatcc
//
LOCUS       DAZKOW010000148         4320 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000148 DAZKOW010000000
VERSION     DAZKOW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.152"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="ACG912_002724"
     CDS             complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="ACG912_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFQ0513988.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTE"
     gene            complement(734..1573)
                     /locus_tag="ACG912_002725"
     CDS             complement(734..1573)
                     /locus_tag="ACG912_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFQ0513989.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1758..2645
                     /locus_tag="ACG912_002726"
     CDS             1758..2645
                     /locus_tag="ACG912_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFQ0513990.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3239..3934)
                     /locus_tag="ACG912_002727"
     CDS             complement(3239..3934)
                     /locus_tag="ACG912_002727"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFQ0513991.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(3918..4316)
                     /locus_tag="ACG912_002728"
     CDS             complement(3918..4316)
                     /locus_tag="ACG912_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFQ0513992.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1430 a          854 c          640 g         1396 t
ORIGIN      
        1 tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
       61 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
      121 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
      181 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
      241 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
      301 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
      361 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
      421 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
      481 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
      541 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
      601 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
      661 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
      721 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
      781 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
      841 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
      901 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc
      961 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     1021 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     1081 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     1141 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     1201 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     1261 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     1321 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     1381 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     1441 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     1501 tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa
     1561 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
     1621 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     1681 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     1741 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     1801 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     1861 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     1921 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     1981 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     2041 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     2101 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     2161 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     2221 ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggagaaaat ttctccaagc
     2281 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     2341 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     2401 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
     2461 tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc
     2521 tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc
     2581 gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca
     2641 aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat
     2701 tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt
     2761 tgtaccaaaa caaatcactg gatttgttac aatcatgagg atataattta aaaaagtttt
     2821 ttatttctat cacatgaatc aatgcgccat taagtagatt ctttcatgct tccctatttt
     2881 ttcacttgcc gtatcgcttc aaaaacaact atcataaact caaactcgac tgaaaagtta
     2941 ttttaaaaaa gactaaaaat atcccttctg gacttgttct tcttattctg ttatactaga
     3001 gaaacacgaa agatattttt atgcgacact tttcatttgt ttttatgaaa tttattcaaa
     3061 actggcgtaa cttcagagtt acaaggatgt attagaggtt agggaataca tcgtttaaat
     3121 gagaaataag taatagttag taatagtcac ccaaacttta aattaatgaa tattgaatcg
     3181 tcagatagcc tcctcgagtt ttcgagaagg ctatcgttta ttttatggag aaattgattc
     3241 agaagtaatc agcgaccttt tttagttcac caatcgtttc tgcttggaaa gtttgcaccc
     3301 aatcttcttg gaagcccctc actgctttat caatcgaggg catctctaat acttgatcta
     3361 gtagctcttt acctaacgtt atcgcatgag ctccagatcg aatggctgta gtgacttgtt
     3421 cacaattttt gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag
     3481 tccgcaacga atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat
     3541 aaggtgcaat ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa
     3601 tagctgtacc ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg
     3661 cttgattcac cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag
     3721 catcctctat catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc
     3781 cgattatttt tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca
     3841 gaattgtagg atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac
     3901 tttttgtatt tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct
     3961 tgatcgattt taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac
     4021 attgttcctg gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat
     4081 tcattcaaat tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca
     4141 aaacagagct tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa
     4201 caataatctg ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc
     4261 ctgaatactt ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatctct
//
LOCUS       DAZKOW010000149         4297 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000149 DAZKOW010000000
VERSION     DAZKOW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.153"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..792
                     /locus_tag="ACG912_002729"
     CDS             <1..792
                     /locus_tag="ACG912_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFQ0513993.1"
                     /translation="FCGYSKTEPRHSFGPAIREQYLIHIVLEGEGYYSINDQKYFLKK
                     GQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYYLKEIGVGKNHLTFKVENLNSF
                     KGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSASYLNEGIITKKMNPFVRQTIEI
                     IRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIKEYISEIKISIANDLLSSTEY
                     SIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLGLGIINGE"
     gene            889..3138
                     /locus_tag="ACG912_002730"
     CDS             889..3138
                     /locus_tag="ACG912_002730"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFQ0513994.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3141..>4297
                     /locus_tag="ACG912_002731"
     CDS             3141..>4297
                     /locus_tag="ACG912_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0513995.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
BASE COUNT         1501 a          610 c          791 g         1395 t
ORIGIN      
        1 ttttgtgggt attcaaaaac agaacctaga catagttttg gaccagctat tcgagaacaa
       61 tacttaatac acatagtgct agaaggtgaa ggctattatt caattaatga tcaaaaatat
      121 tttctgaaga aaggccaagg atttgttatc ccaccaggag tatctacttt ttatcaagca
      181 gatgaaaaaa atccgtggtc atatgtatgg ttgggtttaa gtggcaatct tgtagaatac
      241 tatctaaaag aaattggtgt tggaaagaat catcttacct ttaaagtcga aaatttaaat
      301 tcatttaaag gattaatttt tgaaacttta tcgtatgatc aggacaatct agtcagtgaa
      361 ttgtccagac aaaaacaagt ttataaattt ttagaactcc tcttaaattc agctagttat
      421 ctgaacgaag ggataattac taaaaagatg aatccttttg ttcgtcaaac aattgaaata
      481 atacgtgaga atgcgtatca aaaattatct gttaaagata ttgctgtcag tctatcgata
      541 cacgaaaatt atttatcacg attgtttaag aatgatatgg gaatatcaat aaaagagtat
      601 attagtgaaa tcaaaatttc tattgcaaac gatctgttat catcaactga atattcaatc
      661 cagcaaatca gtgacaagat aggattctca acagtacaat ctttttctaa agcctttaag
      721 aaagcaaatg gtgtttcacc gggagtatat cgaaaacgta gattaggtct aggaatcatt
      781 aatggtgaat aaactaaaca tttgattttg ataaatattc gttactttaa acgcttacta
      841 tttatgtgag cgttttttat aatgcaataa agaaacggag gagagcttat gtataaggat
      901 aatgaaatga atattattta tcgatcagaa aaccaacaat ttcatttgtt taatcaggag
      961 ataagttaca tttttaagat atcggaagat ggtaaattat tgcatttata ttatggtgag
     1021 aacttaccag ataatgacta tagtcatcta attgagatgc atcatcggcc tatgacaacc
     1081 tatagaaatg aaaacgattt gctatattcg ttagagcact taaaacaaga atttcctgaa
     1141 tatggtacaa cagattttag acatcctgct atttcattac tacagaaaaa tggctctcgc
     1201 ttatcagatt ttgtctatca agggtacgaa gtggaagaag gaaaacctaa actagaaggt
     1261 ttacctgcta cctatgttga taataatgat gaaagcaaaa cattgaaagt ttatatttta
     1321 gattcagtta caaatgttca agtagaatta ttatatacaa tttatagaga ttatcctgtt
     1381 attactcgta gcgcgcgagt tatgaataaa ggaaaagata ttcaaataat cgagaatatt
     1441 agcagtttat cattggattt acccgatgca aattatgaat ggcaacaatt atctggtgct
     1501 tggggtagag aaagaactat taagtcgcgt actttacaac aaggaattca atcaatcgaa
     1561 tctactcgag gtatttcaag ccatcagcat aacccatttg ttactttaaa aagaaaaaat
     1621 actgatgaaa atcaaggaga agctattggt gcagcactag tttactcagg aaacttctta
     1681 attcaagcag aagtagatac atgggatgtt actagactac aagttggcat caatccattt
     1741 ggatttaatt ggaaactcaa tccaggagaa atatttacag caccagaagc gattctggtt
     1801 tattcaagta agggtttaaa tgccatgagt caaacttttc atcaactatt tagaaaacgc
     1861 ttagctcgtg gtaagtggcg agaaaaagac cgtcctgtct taattaataa ttgggaagca
     1921 acatattttg attttgacga agaaaagttg gtaaatattg ctaaaaaagc atttgatgtt
     1981 ggtattgaat tatttgtatt agatgatggc tggtttggag aaagagaaaa cgattgtgca
     2041 ggattaggag attggcatgt aaatcctaaa agactaccta gaggaatcgg cagcctttca
     2101 gaaaaaattc gtagttttgg tatgaaattt ggtttatggt ttgaacctga aatggtgaat
     2161 aaggatagtg agttatatcg cacacatccg aattggatta tttctacacc taatcgaaaa
     2221 cctaatcatg gtaggaacca atatgtatta aattttgggt tagatgaggt tgttgaaaat
     2281 attttcgatc aaatgtgtaa aatcattgat gaatcaaact tagattacat taagtgggat
     2341 atgaatcgtc cattgactga tgtatttgac agtcacttgt cagcagatca acaaggagaa
     2401 gtattccaca gatatgtact aggcgtatat cgcttatatg aaaaacttat aacaaaatat
     2461 cctaacatac tatttgagtc ttgttcatca ggtggtgggc gctttgatcc tggaatgctt
     2521 cactatgcac ctcaagcttg ggctagtgat gattcagatg caattgaaag gttaaaaatt
     2581 caatatggaa ctagtatgct gtatcctctt tcgtctattg gtgctcatgt atcaattgtt
     2641 ccaaatcatc aaacaaaccg tataacacct ataaaaacta gaggaaatgt agcttttttt
     2701 ggtgcatttg gatatgagct tgacttaaat gaattaagtg aaggcgattt gaggattgta
     2761 aaagaacaaa ttgcctttta taaaaaatat agaaccataa ttcataatgg tactttttat
     2821 agactattat caccattcga tgatgataat caaaccgcgt ggatggttgt tagtcatgat
     2881 aagaaaacgg ctattgttgc tcattataaa acattaaatg aggtaaatgc tccttaccgc
     2941 cgactgaaat taaaagggtt agataatgac ggactttata atgtaggaca aaatagtttg
     3001 cgatctggaa tggaattgat gagagcagga ctagtctgtt cagatgcatc tagtggacaa
     3061 ataaatgatc agaatcgacg cccagaaaca tttgattttg attccaagat ttgggttata
     3121 acacaggagg aaaagtagag atgaataacc aaaaaagctt acttcaaaaa ggagcaatct
     3181 taagtatttc actaatgtta acaagctctc aagcaattaa tggtgttatt ccacaaatga
     3241 aacagtcttt ggggatatca caaagtcaaa gtgaactttt aggaactgca ccaagtataa
     3301 ctgttatttt gtttatctta ttatctagtt actttgccaa aagaatagga atgaaaaaga
     3361 caataatttt aggtttatta ttagctggta taggaggaat tattccagta tttacagcta
     3421 ccttccaaac cgttcttatt agtcgagtaa tattaggtgc cggtttagga gtatataatt
     3481 cattggctgt gacatatatc agctctttat actctggcaa tactagagca acgctattag
     3541 gtattagaaa ttcaatggaa gctttaggtc agacaatcct tatttttctt gctggagttt
     3601 tagtgaacat taattggacg gcttcgtttt tagtctatgc aattgcgttt cctgtagctc
     3661 tcttattcgc gctaaaagtt cctgaaatta gagatgaaaa tagtgacata cctgaaaatc
     3721 atgtaaaaga aaagatgaat ccaatggttt ttgccctagt tctatttgca atcttactcg
     3781 taatgaatag tattgctatt tctgtacgat ttgcatcaat tgcaactgaa attaaagggg
     3841 taaactttaa tgctagtaac tatttagctc taatgcctat cttaggaata ctagcaggat
     3901 ttatgtttgg accaataaat aagtggatag gaaaaggaac cctatacttg ggaattatat
     3961 tctttataat ttcaaatttg ttaattgctg tgtcaaacgg taatatgacg ttcctgctaa
     4021 cgggtttatt tttatcaagt ataacaggat catggtgctt cccatttatc tttagtaact
     4081 tagataaagt cactacaaag aatacaatta attttgctac ttcattgatt tttattggct
     4141 gtaatattgg taattttata gctcctattg ctatgcaatt agcacaattt ctaacaagaa
     4201 gtacaaattt gactgctcca ttttttgtat tcgcaggaat atttattgtg atcctatttg
     4261 taacattttt tgcgacaaat aaaaaaaata ctagagt
//
LOCUS       DAZKOW010000150         4282 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000150 DAZKOW010000000
VERSION     DAZKOW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.154"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..1257)
                     /locus_tag="ACG912_002732"
     CDS             complement(37..1257)
                     /locus_tag="ACG912_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFQ0513996.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACG912_002733"
     CDS             complement(1277..1888)
                     /locus_tag="ACG912_002733"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFQ0513997.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACG912_002734"
     CDS             complement(1931..2863)
                     /locus_tag="ACG912_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFQ0513998.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACG912_002735"
     CDS             complement(3284..4144)
                     /locus_tag="ACG912_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0513999.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAZKOW010000151         4115 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000151 DAZKOW010000000
VERSION     DAZKOW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.155"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..452
                     /locus_tag="ACG912_002736"
     CDS             <1..452
                     /locus_tag="ACG912_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514000.1"
                     /translation="QGGNLMIKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEEL
                     KCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTD
                     VEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(513..878)
                     /locus_tag="ACG912_002737"
     CDS             complement(513..878)
                     /locus_tag="ACG912_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFQ0514001.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(954..1199)
                     /locus_tag="ACG912_002738"
     CDS             complement(954..1199)
                     /locus_tag="ACG912_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0514002.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1269..1523)
                     /locus_tag="ACG912_002739"
     CDS             complement(1269..1523)
                     /locus_tag="ACG912_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514003.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1687..2010)
                     /locus_tag="ACG912_002740"
     CDS             complement(1687..2010)
                     /locus_tag="ACG912_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514004.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2197..2270
                     /locus_tag="ACG912_002741"
     tRNA            2197..2270
                     /locus_tag="ACG912_002741"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2231..2233,aa:Ile,seq:cat)
     gene            complement(2655..3152)
                     /locus_tag="ACG912_002742"
     CDS             complement(2655..3152)
                     /locus_tag="ACG912_002742"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFQ0514005.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3192..4001)
                     /locus_tag="ACG912_002743"
     CDS             complement(3192..4001)
                     /locus_tag="ACG912_002743"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFQ0514006.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT         1379 a          727 c          722 g         1287 t
ORIGIN      
        1 ttcagggggg gaaccttatg ataaaaaaac tatttttact atcaatatta acgctagcaa
       61 tggcaggttg ttctgactct ggcttagcaa ctgaactgat cacaaaatca actgaagcca
      121 cgacagagga gttaaaatgt ggaaacgatc acagtgacta cagaggctta aatcgacgat
      181 gcatgggaat attaggcaga agcaattgta agcatcgatg tagtaatcga agaaggtgta
      241 gaagaagcaa cagaaaaatc aaaagaaaga tcaaaagaag caatctgtgc tgtgcatcgt
      301 atacagacgt cgaagatgcg gtacttttaa accacgtcag gagatgttca tttatgtcta
      361 gggaagttgg cgatgaacca aaactagagt acgcttttag ttttgcacgt gaggatgaag
      421 ataaccctaa aacttacgac attttagctt gatctaagat ggttaaagct ttttaatgaa
      481 gtaagaggag atagaaggct tttctagtta tattaggtaa gataaattcc tttttgctcc
      541 aaatttgcta aacattctgc tagataaact tcatcgtgtt ccggataaat cggcacgttg
      601 cgctctacag taggtaaatc aaaaaatgct tcgcatgatt tgccacgata cataggattt
      661 ttccactctg gtgctgggct gtaaccacgt ttttccattt catccatgac aagctgatga
      721 tattggaata atctataagg agaatagtga aaaacataat ccacagtagc atgtttcttt
      781 ccccagccat ttcctcttaa tgcacagcat tcccgatgtt gtcctaaaag ctgttgacga
      841 ggaagctttg aaatcaattt ttcatgccat aatcgcatag aaatgtctcc tttatatata
      901 gattgttaac cttagacgtt aaatgaaaaa accagcacca aggctggaaa aaatcacttc
      961 agtttgcttg ctgtttctga gcgaccaatt aggtaatcaa cggaaatatc gtaaaagtca
     1021 gcgatataga ttaattcaat gatgcttgga atttcttctc cttcttcgta cttacgatat
     1081 ttgaataaag gaatacttaa cgcgctagaa acagctactt gagataaaga tcggccagtt
     1141 cttaagaaat tcagacgaca acgtaaagta tccagtaaaa tagcttcttc gatactcatt
     1201 taatcacctt tcattatagt agaatagtta taatgactat agaaagctac acatgcaatc
     1261 aatagtactc aatgtttttt atttaaacgt atcaaataaa gaatgacgat caataaagac
     1321 aaaaccagtc ctgataattc ttctataaaa acactatcgg gttcaaagta gttgtaatta
     1381 aaaactttcc acataaagta gatagccgga agagtcaaaa tagctaaata aaaccgttct
     1441 ttgttccaat tcataagata aacaatactt gcgaccagtg cgaccgcagg cacccaaaac
     1501 aaaactctca ccaacgtaat catgtaacgg actcctcaac gattcatata ataaaagata
     1561 acatgttatt gcatacatca caaatgaaat gctaggaaaa taaaagaaga atagatcaat
     1621 gaaaaatgaa aaagcagaac agttttctgc tgataagcag gaactgttcc gctgaatgtg
     1681 tcttatctat cgagaaaaaa tcaatggatt gatatctaag taattcaata catataaaaa
     1741 gaggacgatc gcattcacta aaaaggaaag aatgatgatc ccatagcgaa tggaaacata
     1801 tttaacaaat acttttgttt gaatacgggc atatcttttt cctttatagc tgagcatcat
     1861 gacaagaagc aaaagggtaa tcacaccttg aaaaatcatt ccaataaaca gaattcctga
     1921 ggtttgataa tttaattgga gaaaataagc tgctgccgca actaaatcga ataaaatcgc
     1981 aataaatggc aaaataatca ctcctaacat tgagaataaa taaaagaata acattttttc
     2041 taaaataagc aaataaaaat taaagtaaca aaatagttat gacacaagta aaagaaataa
     2101 gaataacaat attattttac ttatattttg aaacttgtga gagtttgtca ttcgtgctat
     2161 agttgaaaag taacaaaaat aaattttatt tatgatgtac ctttagctca gttggttaga
     2221 gcagacggct cataaccgtc cggtcgtagg ttcgagtcct acaaggtaca taagaggttg
     2281 tgaaaaagct gccctgcatc aagtaataag aacagccaag taaaaacagc ttcttatgtt
     2341 tttacttggc tgttcttatt actgcagatg caagcttttg aataccgttt aaggaagagg
     2401 ttgtgaaatc agcgttttga cctgagtatt agcttaacaa acggaaaaat agttcctcat
     2461 attttgccgt ttgttaagct aataccgagg gtcagctgat tgaacaccat ttaagaaaga
     2521 ggtcgtgagc ctgccgtttc actctgtgtc ataaaaaagc caatctactt tgatagaaaa
     2581 agcacgtagg actaaaacag ttctgcgtgc tttttttatt gtgtgttaaa tatcgtaaat
     2641 gaagatagat atttttatga ttcgacgata gagacgttga aatctttcaa cagctctaaa
     2701 gcggattctc cgtgttttct actggagact aacattggat caacaaaaaa gtctgcttca
     2761 ggaaaagcac tctgcaggcc tatcagatta gagagtacac acaagtttgt ttcaatcccg
     2821 actacttcga tctctttttg ttcttctttg ctttcaaata aagtttcctg gatttcagct
     2881 aaagtttcag gtggaatcgt aaagtaatac tttttaatca ctcgttcatt ttcttgtgga
     2941 gaaagaagag ggatcaattg caaatcttct cgttcttctt ctttgtcctt cagttcaact
     3001 ggtatatccc gtgtgaagag aatatattca ttattttggc gtgctttagc taatctttga
     3061 gcaatacgtt ctgccaattg atctgactct ggaatataaa attcgctgtt tggatcaaga
     3121 atatgatttt gcatatcaat aataactaac attttctttc ctcctacatt catatcgtca
     3181 ttttattctt tttaatagaa gttttttaaa atataagcaa caccagcttc ttcattcgtc
     3241 agtgttactt ggtttgcaat ttcttttact tgtcgctgtg cattccccat cgcaatactc
     3301 aacccgctgt aattcaacat agcaatgtcg ttttcttgat cgccaaaagc catgatgtca
     3361 ctcgattcga ttccaaggta atccgccaga aaaacaagtg aatgagcttt agtgatacct
     3421 gtaggcgtga tttctaagaa atgcggatca gagaatactg attggatacc atctgcttgc
     3481 agatctcttt gtagcttctg tagtttttct tcactcttat ctgttaatgc taatttaaga
     3541 tagctaagag aaggatcttc caacatattt aaaatttgat caaaagaatg gttctgtaaa
     3601 ttgaaatgtt cataataagg gtcatctttg actagatttg attcatcgaa tgtcagctga
     3661 tcatcttggg tttctcggct agcaatgatg gatattttcg cttcttgagc atagaagaag
     3721 gcttttctca gtgtatcgct atctattttt gaacgtctga gaactttcgc catttttcca
     3781 tccttcattt cagcaatcag tgaaccattg gaagaaatca aatagccacc gattcctaat
     3841 tcggaaaaaa tatctttagc agaaatgctg ttccttccgg tagcgacggt tacgattccg
     3901 ccattttcat caaatttccg aagtgcttgg atattttctt ctggtatctt tccatttcga
     3961 tcaagcaagg taccgtctaa atcaatcgct gctagtttca ttttatcttc ctactttctt
     4021 tttacatatc tattttcctg ttaccttaag attaaatgag aatttaaaaa aatgaaacta
     4081 acttgctttg tcattatgaa ttttttcaaa aaaca
//
LOCUS       DAZKOW010000152         4033 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000152 DAZKOW010000000
VERSION     DAZKOW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.156"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(243..392)
                     /locus_tag="ACG912_002744"
     CDS             complement(243..392)
                     /locus_tag="ACG912_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514007.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            854..1066
                     /locus_tag="ACG912_002745"
     CDS             854..1066
                     /locus_tag="ACG912_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514008.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..3143)
                     /locus_tag="ACG912_002746"
     CDS             complement(1344..3143)
                     /locus_tag="ACG912_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFQ0514009.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3267..3863)
                     /locus_tag="ACG912_002747"
     CDS             complement(3267..3863)
                     /locus_tag="ACG912_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFQ0514010.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1184 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatct cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc
     2821 aaagagattg gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg
     2881 aaataaacta acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga
     2941 tgttgagaaa aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt
     3001 aaaataaaac gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc
     3061 atcagcaaga catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct
     3121 aagctacgtt tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa
     3181 tgaaaaagga aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat
     3241 caatgactaa ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca
     3301 taaatcaagg ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt
     3361 gtatatcgtt tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga
     3421 gcaactggaa gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc
     3481 cagccaatat atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca
     3541 taacgacgcc ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag
     3601 ataaatccag cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga
     3661 atgattggaa taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg
     3721 atggaatcac ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata
     3781 aaataaatcg tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt
     3841 tttcgagagg cagcaatatt cattattcat caactcctta gaggttcttt gttaactata
     3901 ctttactaga aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt
     3961 gtaagttcat cataagtata aaagattgat ctcaaaaaaa catccaacta aagacttatt
     4021 taataacttg ttt
//
LOCUS       DAZKOW010000153         4029 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000153 DAZKOW010000000
VERSION     DAZKOW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.157"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..520
                     /locus_tag="ACG912_002748"
     CDS             <1..520
                     /locus_tag="ACG912_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514011.1"
                     /translation="FLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCL
                     LLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKP
                     PKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPN
                     KNNNFYFYRNKL"
     gene            complement(646..1323)
                     /gene="nth"
                     /locus_tag="ACG912_002749"
     CDS             complement(646..1323)
                     /gene="nth"
                     /locus_tag="ACG912_002749"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFQ0514012.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1330..2028)
                     /locus_tag="ACG912_002750"
     CDS             complement(1330..2028)
                     /locus_tag="ACG912_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFQ0514013.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2296..3480
                     /locus_tag="ACG912_002751"
     CDS             2296..3480
                     /locus_tag="ACG912_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFQ0514014.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3486..3929)
                     /locus_tag="ACG912_002752"
     CDS             complement(3486..3929)
                     /locus_tag="ACG912_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFQ0514015.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1059 a          785 c          655 g         1530 t
ORIGIN      
        1 ttttttgaaa gaaggcttca ttttgagtta tctgatagta tgcttaatta tcataatttg
       61 tctgcaccat ctttttttat cgaaaacaat acctcgttat tccaagaata aaaaagctga
      121 aaaattttgt ttattattaa ataaatttac tttaattgct cctatccttg catttattat
      181 tttttctgta cttttaagca ctactcttaa aggaaaattt atggaaagaa gctctcatgc
      241 aatgattctc acttttttat ggttgttgtt caccagaatc tatatttttc tgatgagcct
      301 gaaaccgccc aaatccatta gtttatgttt agttataaat ggtatttttt tattatcact
      361 tatcattttt attacacctt tagatagata cgtcacttat ttatataatc ctttggaata
      421 ctggacttat tttataggta tattggaagg cattattttc tacatcgggt attttcccaa
      481 taaaaataac aacttttatt tttatcgcaa taaattgtaa taagttacca tcctagctac
      541 tgagtatgta catttacttt ttcatgcaaa aaagataaac gaaacagctt ttctccataa
      601 taacaagata attcatacat ttatttgcaa ttaaagaaac cacgtctaca ggcgtggttt
      661 cttttttagc gccttttctt ttagacgcat ccgatttttc ccatcttgac atattgatag
      721 caaaggacat acctcgcact tgggatttct tgccgtgcaa tgatatctac caaaaaaaat
      781 cagtgtatga tgtgtcttta cccataattc ttgtggtact ttccgcatca gtgtttcttc
      841 tacttccatg accgtagcat ctagtttgca gatcctcagg cgttttgaga cacgttccac
      901 gtgggtatct actgcgattg ctggtatccc gaatgcatcc cccaatacga catttgctgt
      961 ctttctgcct acaccaggaa gagacatcaa ttcttctctt gacgttggca cttgcccatc
     1021 gaaacgttca atcagctgct gtgcacacgc tttgatgttt tttgctttat ttcgatacaa
     1081 accaatcgtt tttattttaa gaatgatttc atcgattgaa gcttctgcta acgcatcggg
     1141 tgtcggaaaa gaggcaaaca aatctggcgt tgctttattc actgatacat ctgtcgcctg
     1201 ggcacttaag atcactgcaa tcaataactc aaatggatta ttgtgcttga gttctccatg
     1261 tgcttcggga aacattccat acatcgtttc tagagcttcc attgtttttt gtttgcttaa
     1321 catattccgc tactcctcca accaattttt cagtgagact ttcggcaatt cttcttgttg
     1381 agctgactgt tctacttctc tttgaagaag ctgctgtttt cttcgttttt ggtcttcttc
     1441 cacttgttgt tttgtacgta gattttttct ttcccacgat agcaaaatgc ggtcaacgta
     1501 attaaaacta taagcctgat ttaatacggc ttcccgcagt gctagcttca aaatttccgg
     1561 ctgataatgg tcttcttcta gccattgtcc gatccgctgg aactcgatcg aagaaagcgg
     1621 gcgtccaaat tcttgttcaa ataattgata cactgactgg atttcttttt cttgtgtttt
     1681 ttcttcgtat tttttttctt gtgtttttaa atactcacct aatagatcat agatggggta
     1741 aagattatat cgatctgctt ttttaccgtt gacaatcgtc gtttctatct tgataaatcc
     1801 atttgttacg agacgatcca gtatttgaaa aatctgatct tgtttgattc ccatatccat
     1861 agcaatcatc tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat gcaactgtag
     1921 aacaaataaa aattcttcag attgtagacc gatcctatga taatttttaa gtaaaagatt
     1981 agatatgact gtttgtccag cgcttaagta attttctaaa ttcaacatga tttctcctcc
     2041 gctcaatcac agtatacgta aaaaaaacat gggaagcaag aacaggtttc ttttctattt
     2101 acttcactga tcggtcagat ttacaatgtc gtctacttga tttttgtttg ttcatgtttt
     2161 tgatgcgtat cttcactttt cggaataaat aaattaagta gatttcgctg ttccttagaa
     2221 tgttgacaag gaaacaaaat tactatacac tatttttgtt tccaagttaa cattattgca
     2281 taaaggagtt tttacatgta cagtttcaca aataaacatt ctaaagtttt tggatacgtc
     2341 tctgtcttat tcactggatt aggatttact atcgtcagtc ctgtacttcc gtttttaacg
     2401 ctgccttatt ctcactccat acatcagcaa gctttctata tcactttgct catgtctgtt
     2461 tatgctctag ctgccttctt atctgccccc attctcggta gcttaagtga tcattttggg
     2521 cgtcatccga tccttattat cagtttatta ggatcaagta tcggatattt gctttttggt
     2581 ttaggaaatt cgatatggat gttgttcctt ggtcgtatca ttgaaggatt gacggcagga
     2641 gaaatctcca ccttatatgc ttactttgcc gacatcacgg aacctaatga aagaaccaaa
     2701 gtttttggat ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc tattataggg
     2761 ggtctattag ctgagttggg gaattctgtt cctatattta ttggagcact ctttactttc
     2821 ttaaatgccg tttatggcta cacatttatg ctagaaagcc ttccaataaa aaaacgttca
     2881 gtagatttaa gtttctccca tgtaagacct tttcaccaat tacaacaatt attcaagatt
     2941 tcatccgtta ttcctttatt gacagcaggt tttgctgttt ggttggcagc tggctctctt
     3001 caatctatct tttctcaatt ttcgattgac gcctttcagt ggaaggctgg gttggttggt
     3061 ttaagcttct ctttgattgg aatcttggat actgtctcgc aattgttcat tatgcctcga
     3121 ttgctcaaaa aattttcaga acagcagatc acaagaatag ggatgttcag tgaaatcctc
     3181 gcctatctat tcatcacatt atctggaatt cttctactgc cgattttgtt tttatttggg
     3241 attatctgtt atggtttcgg tgattctatt tttactcctg tattcaatgg ccaactatcc
     3301 aattctgtct cagataacca gcaaggtctt gtaatgggcg gtacccaaag tatacaatct
     3361 ctatcgcgcg tcatcggccc gctaatcgca ggacagcttt atgctgcagc accatttctt
     3421 ccgacggtat tcggattttt gcttatgatt tttgcctatt atcactacaa taaaaaatag
     3481 tagcttcatt tgctacttcg tataaattct ctgtatcgac cgataaaacg aaatactgtg
     3541 tcaaattctt cttttgacat attttcaaat acagtagcat ctcgctgtac ccatttttga
     3601 tggagttgct cgtgtctatt ccttattttt tgtccgattg gcgtcaattg aaaatagatt
     3661 tcttttttat tctcttcgct ttgataagtg tttatgatcc ctctagctat cagttttttt
     3721 gttagcttac tgatggctcc tctagtcatg taactagcat tcgctaattt tgtcacgtta
     3781 ggatcaggga ttttcccgat atgttcaatc acttctagtt catttagagt aaatcctgca
     3841 aattcttttt ccatttctgg tttattacga tgaataatct cattagctaa atccagatat
     3901 tcttctatta gcagttgtct tttatccatt tctacacctt ctttttcttt cattatattg
     3961 tttttttgtt tcctattcaa caaagataaa aatattttgt aggaaaaccc aatctgaaac
     4021 caagaaaaa
//
LOCUS       DAZKOW010000154         3968 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000154 DAZKOW010000000
VERSION     DAZKOW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.158"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            44..2713
                     /locus_tag="ACG912_002753"
     CDS             44..2713
                     /locus_tag="ACG912_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0514016.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(3168..3914)
                     /gene="truA"
                     /locus_tag="ACG912_002754"
     CDS             complement(3168..3914)
                     /gene="truA"
                     /locus_tag="ACG912_002754"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFQ0514017.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         1121 a          793 c          754 g         1300 t
ORIGIN      
        1 ataaactgaa tacaacttca tagaaatttg gtggaaaata aatatgaaaa aaaatttaaa
       61 gtcattcttt agagaaaacg gacgctacgc agcagctagt ttcctgattc cgtttttgat
      121 tttagcactt gtttatctaa gtatcggaat ctatcctgga agcagcagaa gtattttggc
      181 aagtgatgcc ttttcacagt tttctaattt tcatgccagc ttccgaaatg ttcttcttgg
      241 aaagcaaagt attttttata catggaatgc ttctctagga ttgaattatt tatctttaat
      301 ttcttactat ctaggcggga tcttcacgcc actggtgttc ttttttccaa accagatgat
      361 gccagatgca ctgtatgttc tgactttgct taaggtcggc tgtgccggat tggctttttg
      421 gtttcttgca cgtacgttca agattcctcg ctgggggcat gttgcactta gcgtcagtta
      481 cgcattgatc tcatttatca ctgcacattc ggagatcatc atgtggctgg atgcattttt
      541 ttatcttcca ttagtgattt tggggattca tcgtgttatg gatcttaaac ggccgaaact
      601 tttatttatc agttatttac tcttgttttt atccagtttt tatatgggtt ttatgattgg
      661 cgtattttct ttcttatatt ttatcgcccg tctattgaca aactggtcag aatataaaaa
      721 aacgattgtt ccttatggta tcacttcttt actggcaggc ggcgcttcaa tgatcattgt
      781 attgccagca gtgctggatc ttcggtcaaa tggcgaggca ctgagtcaga tcactacgtt
      841 gaaaacagaa gcgacttctt atctggatct aatcatgaaa aatatgattg gtgtttatga
      901 cacgacaaaa tacggatcga ttccgttcgt ttacgcaggt ctgctaccat tagtcctctg
      961 tctgttttat tttgccagtc ggaaagtacc gttgaaaaat aaatttttgt acggcagtct
     1021 tttcgccata cttatcgcaa gtttttatct tgttccattg aatttgttct ggcatggtat
     1081 gcatgcaccg aatatgtttt tattccgtta tgcctatctg ttctctttct tagtgctcct
     1141 gcttgcagga aaaggatggg aaaagataga acaagacgat cgtgggctgt tgttaggaat
     1201 cattttggtc gtagctgctc tctttgcggt ggcatggggc atcaaacctg aaggaagcta
     1261 cacctatgtg acgatgactt cctttgtatt gacagtggtt ttcttaggga tttatgcttt
     1321 tgctgtggga ttttatcagc ataaaaaact ttcattcaat catctgagta tattgttatt
     1381 attgatcatg tctgctgaag cactggtcaa tacgaacagt atgatccatg gcattcttga
     1441 tgattggaat tatgcttccc gcagtttata tactgatcct tatcctgact tgaaagctct
     1501 tgtaaatgaa acaaaaaaag attccgactc gttttaccgc ttggaaaatc tggatccagt
     1561 atctgcaaac gattcgatca actatggtta cagtggtatc agtttgtttt cttctataag
     1621 aaatcgtaat tcttcctctt acctggataa attaggtttc cgttcaagag ggacaaattt
     1681 gaatatccgt tataacaata atactttgct gatggatggg tttacaggaa tcaaatataa
     1741 catttctaaa aacgaaagca cgttcagtaa atatggcttc cgccaagctg gtcaaagcgg
     1801 cgactataca ctgtatcaga atgtcaatgc attgccacta gcatttactg caccgttgtc
     1861 cgttaatgat gttgagcagc cagtgacaga taatttgacc agtcagacaa acttgatcaa
     1921 tacattgtct ggtctaaatg aacagtattt tacattctat acacctactt tgaagaatca
     1981 gttgaatacg acgatcacaa agacaacgac tggcgtcact tactcagaaa cagcagataa
     2041 ccagccaaaa gagctcactt gggaagtcca tgtgcctgca aatacacaag cttacttaag
     2101 tttgttccca accaattttg cgcaattaga aagttctagt gcgacgatca ctgtcaatgg
     2161 tacatcgcga aaaacgcaaa tcaacatttc tggtcaatac tatgatcttg gctattatcc
     2221 tcaagagaca acagtgacat ttactgcaag tttttatggg acgaaagaaa taagtttcat
     2281 ggagcctaaa gtcgttggtc tagatgttgt agcctatcaa gccgcaatga atcagatcaa
     2341 agaaaaaggt gtcgaaatgg aagccactgg cagaaaagct tctgggactg taaataccgc
     2401 tgaagattcc atgttggtga caactattcc ttatgacaaa ggatggaaag ctaaaattga
     2461 tgggaaacca gtcaaggtca gcaatttcaa agacgcattc ttgatgataa aagttccagc
     2521 aggtaaacat actgtcactt tcagttactt gcctgaagga tttacgatag gtgcaatcct
     2581 ttttgtccta tgtattgcat tgtttattct ttatgtctgg taccttgacc gcaatcgccc
     2641 cttttatggc acgattgatc cagcaataga agaacaacca cgaaacacac acagaagaag
     2701 acgtagaaaa taaagaaacg atggaacctg ttcagactgt ccgcaccaag ggcaatcaaa
     2761 acaacgttcc atcgtttttt ggttacagaa aaaataggta acaaaaagat gattcactta
     2821 tcaacgcctc gtccacaagt cacgatattc atcaaagatg agaagctgtt ttttgaaatt
     2881 gctctttgtg actcagagtt aaatgacagg ctcacgacct ctttcttaaa cggtgttcaa
     2941 tcagctgacc ttcggtatta gctcaacaaa cggcaaaata tgaggagcta ttttcccgtt
     3001 tgtttctcta atactcaggt caaaacgctg atttcacgac ctcttcctta aacggtgttc
     3061 cagaagtagc ttcttcgaaa ataagccgaa attttgcaaa attttgagaa gtaattttcg
     3121 taaatacctt cttatttctt gaagctgacc acttctatca taacctctta ttcatatttc
     3181 acttcataca aataaagtcc ttcagggtgt gctgtaggtc ctgctgcatt acgatctttt
     3241 ttcgcaataa tatctgggat actatcttca ggcaatcggc cattgccaat tttcaaaagc
     3301 gttcctacta agatccgtat catcttatac aagaagccat ttcccctaaa tgtaaacaac
     3361 aattcgtctt ttgtttcatt tacctccatc ttagcctcgt agatcgtacg tattttatcc
     3421 tctatcgagc ttcctgatgc acaaaaagag gtgaaatcgt gagtccctaa taaatcagga
     3481 agcgcccgct gaattttttc caaatcaagt gggtaaggaa aataacttgc ataatggcgt
     3541 cgaaaaggac tgcgtggttt ccctatatcc actcgaaatt gatacgtctt ttctttgacc
     3601 aaatatcttg cgtggaagtc ttctgaaaca atttctactt gtcttacagc aatatcctct
     3661 ggtgactggg tatctagcgc gaaacgcatt cgttctaacg gacgctcctc tggataatca
     3721 aaatggatta cttgcccgat cgcatgtact ccggcatctg tccttcccga accaaagacc
     3781 gtgatttctt ttccatttgc cattttttgt aatgtttttt ctacttcttc ttgaacagta
     3841 cgcccatttg gctgtttttg gaatccatta aaatttgttc cgtcgtacgc aatgattgct
     3901 ttgtaacgta ccatgttttt ctctcctttg ttcctaaaag gttatctgga ataatgattt
     3961 ttctataa
//
LOCUS       DAZKOW010000155         3929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000155 DAZKOW010000000
VERSION     DAZKOW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.159"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(579..1136)
                     /locus_tag="ACG912_002755"
     CDS             complement(579..1136)
                     /locus_tag="ACG912_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514018.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1315..1926)
                     /locus_tag="ACG912_002756"
     CDS             complement(1315..1926)
                     /locus_tag="ACG912_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514019.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2039..2296)
                     /locus_tag="ACG912_002757"
     CDS             complement(2039..2296)
                     /locus_tag="ACG912_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514020.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2893..3171)
                     /locus_tag="ACG912_002758"
     CDS             complement(2893..3171)
                     /locus_tag="ACG912_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514021.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3205..3456)
                     /locus_tag="ACG912_002759"
     CDS             complement(3205..3456)
                     /locus_tag="ACG912_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514022.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          803 c          521 g         1411 t
ORIGIN      
        1 gcctccttat tccgaataaa tggtgtccaa aagcttgcat ctgccagatt atctcatttc
       61 ttatgacaag ctaagcaaat tatctccacc aaataattta ttcatatttt tgacttgcgc
      121 atactaacta attcttaaat tttcatacaa aatttctcaa tttcccttaa cgagtaatat
      181 ttatgtacgg tgattctcac tagtatccca atcacttttt aagttatccc gatttcaatt
      241 ctacttcaaa aatagttgct tttatgtatg ctgttagttg attagatggt catttgttga
      301 gctaggtcgt ttattttaaa caagttgttt ctttagctta tacctatctc gtctaccaaa
      361 cgcaagatgg caaacacaaa tgcatacgca tctgtatttg ccactttgat tcgtataaag
      421 ctaacaacta ttaactcctc tcagctaata gttgctctct tttttattca gattcatttt
      481 tgctgatcac agcctagtat ttcaatggtt ttgaacacta aaccaaattg ttccattaca
      541 acattcactg gctgatgaaa tggattagta gatttcgatt attcaaagcg attttctatt
      601 ggtggatcgt gttcccaact aataagacgg attccaaacc tatcgtacag atcttctaaa
      661 ccacttataa attttgcacc tatttcaggt gttttattgc gtggatattc tattgttacg
      721 ccattaaatg gataccaatt aggattaggc tctctttttt tctgtacatc tcccatagaa
      781 tagttagcac tttctgggac ataatatatt cttcgaccat cctctaattc atctaataag
      841 atatttcgtg atcgtctatc acttgctaaa tgtagacgcc acgtgcgata ccatgccatg
      901 ctacctgcat ctgcagaaaa tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc
      961 ctttccccat ctacttcata ttctaatcta aaatcaaact cccctcgggt aatttctggt
     1021 cttggtggct taggacccaa tattattcta cttaaaaccg cattcgttcc cagatgaaac
     1081 acagctataa caacaataat gattaatatt ttgtttctat ttgataaatt tctcattatt
     1141 cgcacatcac ctctacattc tcacatatta gttcgtaata ctcctttaat atcaaaatat
     1201 ctactggtcg taataatatt cgctatgaat tcctactact cgtataagaa aggccaaatc
     1261 atcagctttt acttgctgat gatttggctt gtttctattt atcgtaattg ttttctaatc
     1321 accaaaattg ttaacaatcg gttcagatat ttcccagttg attaaggtaa ttccgtattg
     1381 tgtcaaaact tcttgcgctt ctccgaaatt aagtacagtt cttatgtatc cactttctcc
     1441 taatataaag tctacctctc ttttaaaacc ttcctcgaga tttctaaggg ctatatgtgg
     1501 ttttaatgac attgattgta cattttcccc catataatat tgcgcacttc ctcgactaaa
     1561 atagattcct agatcgtccc caaaaggtag atcttctcta ccactagcta atctacttct
     1621 ccatcttcgt tctcttttca tagaacctgc gctgaactca cacccgttaa actcagctat
     1681 gacagtatct tcaattatgt taatctgtcc atctagttcg tattcaatcc taaatggaaa
     1741 ttctccatag gtgatttccg gttcgggtat tttaggtgag attaatgcaa agaaaggaaa
     1801 tattaagctt ggcaacatca aaaaaactcc tcctatgatc acaattcccg atgtaatcca
     1861 aaatataatt tgtacaattc tcattgatcc attgtattcc attttattcc ccctattact
     1921 attcatatcc gcctatctat atcgatcttc caatgcagag ataattgctt ccataccttg
     1981 attttttact gagttaccta tgcgagatat cattctccac taaggtgcat catattgtct
     2041 atctaacatg attaattctc caataggtgt atactcctcc aaaaacaaca tttaatgcat
     2101 ctccctcgac aacataagct accatgtttg ctgtatatgt gtttgagtct aattcataag
     2161 cagataaatt tactgtcgct ggattaaata taatcgcatt cgtatttata gctactgaat
     2221 ttgctgcttc agcaccacct tttgaatgtc cgatcattgt gatttcaata gtaggattta
     2281 cgtggacagc cctcaccgtt taccctgacc attatagaaa tcgcttactt ttttgaacta
     2341 cgcaacaaag gatacaatgc tcctaacttc actgtctcca tttttgtcca ctaaggaata
     2401 taaaaaatag gcattaattc atttaattcg gtcacaacac gttacctttt aaatcattat
     2461 actgtatctc atacattatt atcaataaac tattataaaa atgcaaaaaa tgctttatca
     2521 atctattttg taattggtca taatatcatt gaaatcatac cctgtatcga tttttcctat
     2581 gaatctcgtc tatttgtgat tgacgaaaaa gaggcaagac agacactgga atggtaattt
     2641 tacccactat tttgctccaa ctatttgtaa tcgtacccac ttttgaaata ccgagatgat
     2701 cattttaatt gatatgctga ttcccaagca aaaccccaag aaattaaatg ctctatcgca
     2761 tctagcttct tgggttttgc ttatataaat tccaacattt aaatttttta tgctgttcgg
     2821 tctcagaaat gccgtttttg cttatatcac aatatcaaga tgtcaaatta ttcgtgttgc
     2881 cgatgacttt gattaatttt tttcaaattg tttattaaga ctaatgctgt ctttttgcag
     2941 ataccaatgt tcgaactttt taacattatc atatgatcct gcaagtttta acttcttacc
     3001 attagatgtt atcaaagtta taattaacct tgcctttttc ccattaggat cttttatttt
     3061 atgaattttc tgcaggtaat ctctcctaat ggcagctatt tttacctcat cagtgctgat
     3121 aaaccattga tcatccacat acatactgcc tgttatgttg tgtttttcca tttcttccga
     3181 gaacgaaaag tttaaggctc gttcttactt ccaaagttga atgagtccat ggattggact
     3241 tagtgtgaga attgagaaca acaataagaa tccatatcct cccgcaaaca tccctaaggc
     3301 tatactgtct ctccatccat aagtaggatt agaagagaaa agagtcaccg tttgtaataa
     3361 tatgaaaagg aataatccag cacaaacacc aaagataaca actccaaaca ttgaattttt
     3421 atacaatgac catttattta taggcttttc tttcatttta cttcccccta ctgttctgtt
     3481 cacacggaat gaattcaaag ttcataagcc ctcctctttg aacatatgaa tacgacttgc
     3541 taactatact ttaaaaaagc ttgttttaag cggttatagg cgagttgtaa tctctattca
     3601 tctcattagc aaataacttg ccgaaaagtc tactgaaatt aaagcagata gtggaagaat
     3661 tatttagcca ggatagacac tatttgtgaa acacatgtct ttacttttga aattctatga
     3721 tgaaaagcct tggaatacag tagctcttaa ttggtaatac tttttccatt ataaataacc
     3781 tcttttcatt ttatatttat cccctctaaa aataatatct aatctaatat tttaaactta
     3841 tatttccctc gctgtgctaa tagagcatta ctaaataaat agaaactaga aaaaagcatt
     3901 acgcctgcca gaataaacca attatttgt
//
LOCUS       DAZKOW010000156         3863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000156 DAZKOW010000000
VERSION     DAZKOW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.160"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(245..466)
                     /locus_tag="ACG912_002760"
     CDS             complement(245..466)
                     /locus_tag="ACG912_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514023.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(564..809)
                     /locus_tag="ACG912_002761"
     CDS             complement(564..809)
                     /locus_tag="ACG912_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514024.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1049..1663
                     /locus_tag="ACG912_002762"
                     /pseudo
     CDS             1049..1663
                     /locus_tag="ACG912_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2033..3034)
                     /locus_tag="ACG912_002763"
     CDS             complement(2033..3034)
                     /locus_tag="ACG912_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFQ0514025.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3193..3459)
                     /locus_tag="ACG912_002764"
     CDS             complement(3193..3459)
                     /locus_tag="ACG912_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0514026.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3449..3805)
                     /locus_tag="ACG912_002765"
     CDS             complement(3449..3805)
                     /locus_tag="ACG912_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0514027.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1431 t
ORIGIN      
        1 ttactatata cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag
       61 ttcattgggt ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt
      121 tatgtcctat ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt
      181 gtaatatgaa gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga
      241 accattatag atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa
      301 atgtttcaaa ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt
      361 aagtccaatc aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa
      421 ctgaagccaa ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc
      481 aagacgctac ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta
      541 tagcaaacaa atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg
      601 cttctattct taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac
      661 tcatttattg ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg
      721 gcatcctgat agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga
      781 acttctttta caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat
      841 ctataatatt tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta
      901 ttataaacta tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa
      961 atagacaaaa atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat
     1021 aaacgttata gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat
     1081 cgatcaaaat gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt
     1141 tatcgaaaaa caatctggag cgacgattag tcatcggccg attttccagg aagctttaga
     1201 ttttgtcaga gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta
     1261 tgacgagatt atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac
     1321 cagtttaccg atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga
     1381 cttaatcatt taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg
     1441 ccaggcacaa ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt
     1501 atacagtgca gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga
     1561 tcttgagaat ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac
     1621 aatttaccga ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac
     1681 gtaattatta attagctttg tcatcaatac agacaatcat tttagattca tctagctaag
     1741 ttgattacat ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg
     1801 tccttattta aaggcattta acattgacaa cttagtataa tgtatcttat gtaaactaat
     1861 aagatttgaa ttttatttta tacacactcg ataatagatc aattttatgg tcccccgcat
     1921 ttacgattgc acaagagaaa cataaagtcg acctattagc atttcttttc aattcaaaca
     1981 ttaaaaaagt ttatacctct gctactaaaa aatgaaatta tctatttaaa atttaaaacg
     2041 gtaaatctga ttcatccaaa ttggaaaatg gatcagcctg tattggagct ttgtatttct
     2101 ccataataac agatatcttt ttttcatttt ttaaaaaata ccttttaaat ttcttatagt
     2161 cctctgaaat caaaatatag cgtgatggat actcatcaaa agaagaatcg tattcttcct
     2221 ttaaaattcc cgatttactt aatttttcta gtacatcaaa tactctatca ctcaagtaac
     2281 tccttaaatt gttatcactt tcccattgaa ctacttttct ataagttgaa ttatcagttg
     2341 aaaagataga attttcatcc ataaacgcat atattagcag caatctttca tcattgtcaa
     2401 attctattgg atcaaaaata tctaagttgt catcaaaatc tttatcagtt ccggtaactt
     2461 ttttgttaat tactgaaggt gtcttgtaag aaatagaatc taaatcaaag cgttttgatt
     2521 ctaaattatt ttgtgctttc tcaattaaat aattcacttt ttttgtgaga aaccttctga
     2581 tattgtcttt gctatatgtt ccataaaaac ccttatcttc taaagagtcc ctgtacttct
     2641 taacttctat atactcttca gccaaaactt gtggaactgg ttcatctaag aaaaaaaccc
     2701 ctacctcgcc tttgctattt ttataaaagt aatcaatttc tgctatagtt cccgatctat
     2761 aattatcaac tggagaaccc aacttatggc caaaaatcgc taataatata tcactatttt
     2821 ctacaatttg tttattaata acactttgcc cagagctatt atttatataa gtaggaatcg
     2881 cattcgtttc ccatcgaatt ggcatcaaaa ttacttttgt attatctgta ttttctatat
     2941 tccatctata tatggtttct tctacttcat tcctctttgc tgctatatca cctggtgatg
     3001 ctataaatac ttttattaga tttatggttt tcatttattg tacctttctc tttatgtctc
     3061 tttaaagtaa tttttgtata aaatttattg acttttacac tataggacaa cttttatcta
     3121 tttagtacaa aaaggcccac taaactaatt gattagttag gagcctatta caggtatatt
     3181 ttcaactcgc ttttaagcaa attctactac cagctttttg cctaatgccc tagcgatatc
     3241 atcaagttta tcgaaagata cgttatcccc tctctcaatt cgagcaatag tagattgagt
     3301 tgtatgtgct ttttgtgcaa gctccgcttg agttaggcct gcttcctccc gtgcatgata
     3361 aagagcaacc gcagtttcga gttttcttcc ttcacggtca tatgcttctg caaaatcttt
     3421 gtctttttgc ttttcttcaa tcaatttttt aatcatcatt tgtgaaccac tcctctctaa
     3481 gttcttttgc atgtttaagt tcacttattg gtgttttctc cgttttttta gtaaatccat
     3541 gggtaatgac ataccttcca ttgaccacat ggaaatagat agctctttgg atgtttgatc
     3601 ctaccttact tcttaattcg tacaaattag tgtctaattt tttcacccat ttcaaccgtt
     3661 gagctactag catcccattc ttttctgttt cttctattgt acgtaaaagc ttcgcactat
     3721 ctttttttgg taatgtttcc aaccattcta aaaattcatc atgaccatta ggtcttttaa
     3781 atgtttcaaa agatattttt tccataccca attatatcat atattgattt tatattatag
     3841 tatataacat aaaaattcga att
//
LOCUS       DAZKOW010000157         3603 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000157 DAZKOW010000000
VERSION     DAZKOW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.161"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..186
                     /locus_tag="ACG912_002766"
     CDS             <1..186
                     /locus_tag="ACG912_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0514028.1"
                     /translation="VEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTV
                     YEGHFVIAFKSGIEMEI"
     gene            329..553
                     /locus_tag="ACG912_002767"
     CDS             329..553
                     /locus_tag="ACG912_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514029.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            568..1437
                     /locus_tag="ACG912_002768"
     CDS             568..1437
                     /locus_tag="ACG912_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0514030.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1418..2152
                     /locus_tag="ACG912_002769"
     CDS             1418..2152
                     /locus_tag="ACG912_002769"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0514031.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            2185..3093
                     /locus_tag="ACG912_002770"
     CDS             2185..3093
                     /locus_tag="ACG912_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0514032.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            3090..3570
                     /locus_tag="ACG912_002771"
                     /pseudo
     CDS             3090..3570
                     /locus_tag="ACG912_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1251 a          521 c          790 g         1041 t
ORIGIN      
        1 gtagaagatg cctccctcag tggcgagaac gagcgaatca atgagctgat tgaatttatc
       61 cgcaagaaca aattccgcac cttagaatat gatgataagc ttgtaaggaa gataatccag
      121 agcgttacag tctatgaagg ccacttcgtc atagccttca aatctggcat cgaaatggaa
      181 atatgaaaac cagaagtaaa tagcccatga ctctacagta gagttgtggg ttttcttttg
      241 cttgctgatt ataataatat tttaataact attgtgttta tcaaccaaat ggtttataat
      301 attcttagcg taaacttgga ggtgctgtat gactacggca gaaatgatta aagaactgtg
      361 tgagcaaatg aatataagtg tttccgaact tgctagacgt attggccaga ctccacagaa
      421 tttcaataaa aaattacaac gagaaacagt aaccttggat gagttgaagg ccattgctga
      481 tgtgctaggt gtcaagtttg tgcaggcatt tattttacca gatggagaag aaataaaaat
      541 atctaacgaa taaaggagga tgtttatatg gtaggtaatg ttattcaaat agtaacagag
      601 aaattatcat ctttgccttt tatagaaggc atcgtattag ggggctcacg tgcaagaggc
      661 acccatacag aaaattctga tatagatatc ggaatctatt acaattcaga ctcatttgac
      721 ctgacagcga ttaatcaaat tgctacagaa ttggatgata aaaatagaaa caaccttgtt
      781 gtgcctcccg gagcgtgggg tgattggatt aatggcggcg gatggttatt tataaatggg
      841 tatcatgttg acttgatttt acgtgatata aaacgggtag aacaaataat caaagatacg
      901 gagcaaggaa ttgttactgc caattatcag accgggcatc cccatggtta tataagtgca
      961 atgtatcgtg gagaattagc gattagtaaa atactatatg ctaagaacga aagcttatgc
     1021 gaattaaaaa agcaggcaga aatttaccct actgctctaa agaaaagctt gatgaacttt
     1081 tttatatttg aagctgaatt ctctttaatg tttgtaaaag caaatgcggg ggtagaggat
     1141 aaatattata ttgcaggcca tgtttttcgt attatttcat gcttaaatca agtactattt
     1201 gcatgtaata atgcttattg cattaacgaa aagaaagcta taaaactgct tgaaactttt
     1261 gaatataaac ctgaaaaata tgccgaaaga gttaatcata tttttgaagt actcggtctt
     1321 tctctttttg aatgctacga tatgaccgag aaactttata aagaagtgaa tgaaattgta
     1381 tcggagataa ataacttttt aaacgaggag agttcagatg aaagaaaaca aatatgatga
     1441 taatatattt tttcaaaaat acagtcaaat gagtcgctcg cagaaaggac tggctggtgc
     1501 gggagaatgg gagactttga aaaagatgct acctgatttt aagggtaagc gtgtgcttga
     1561 tttaggatgc ggctatggat ggcactgtat atatgcgatg gaaaacggtg cttcctctgt
     1621 agtaggtgtt gatatttctc ataaaatgct cgaagtagca aaaggaaaaa cccattttcc
     1681 acagattgaa tatgaatgct gtgccataga agatgtggat ttcccagagg agagctttga
     1741 tgtaatacta agttcgcttg cgtttcatta tgtagcagac tatgagaatt taataaaaaa
     1801 gatatatagg atgctgaagg ctggtggcaa tttagttttt acagttgaac atcctgtttt
     1861 tactgctcat ggaacacaag actggtatta taacgaaaaa ggagaaatac tgcatttccc
     1921 ggtggacaat tattattatg agggcaaacg gacagctatg tttttggaag aaaaggttac
     1981 aaaatatcat agaacactga ccacatatct aaatacactg ctttcaaata gttttataat
     2041 aaatcagatt gtggagccac agccgccaga gaacatgatg gatattccgg ggatggcgga
     2101 tgaaatgcga cgcccaatga tgctgattgt atcggcaaaa aagaagatgt aataatatag
     2161 aaaaaataaa cgaggagtat gtaaatgaga tcagaaaaag aaatgatgga tttagtactt
     2221 tctttagcag aacaggatga acgtattcga attgtgaccc ttgaggggtc acgcgcaaat
     2281 attaatatac ctaaagatga atttcaggat tatgatatta catattttgt aagtgatata
     2341 gaaccgttta tatctaatga tgactggctt aatcaatttg ggaatataat aatgatgcaa
     2401 aagccggagg atatggaatt attcccacct gaagaaaagg gattttccta tcttatgcta
     2461 tttgatgatt acaataaaat tgatcttacc ttattgccct tggaagagtt agataattac
     2521 ctaaagggcg ataaattaat aaaggttcta attgataaag attgtagaat taaaagggac
     2581 atagttccga ctgatataga ttatcatgta agaaagccaa gcgcaaggga gtatgatgat
     2641 tgctgcaatg aattttggaa tgtaacacct tatgttatta aaggattgtg ccgtaaggaa
     2701 attttatttg ctattgatca ttttaatcag attgttcgcc atgagctgct gagaatgata
     2761 tcatggaagg tcggcatcga aacaggcttt aaattaagtg taggcaagaa ctataagttt
     2821 attgaaaggt atatatccga ggatttgtgg gagaaacttt tgtccaccta ccggatggat
     2881 tcctatgaaa acatatggga agcattattt ctatgccatc aattgttcag ggcggtatcc
     2941 ggtgaggtgg cggaaaggct tcattatgcc tatccggagt atgataggaa tataacaaaa
     3001 tataccaggg acatgtataa aaaatacact ggtaaaaccg gctgcctgga tagcacatat
     3061 gccgctgata tagaagagag gcgggaacag tgattacaga aatgaaagca gggcacctga
     3121 aagatatcga taaacccagc gaaccatttg aggtgatagg taagattata ccgaggtatg
     3181 aaaacgagaa ttggaccttt acagaattac tctatgaagc gccatattta aaaagctacc
     3241 aagacgaaga ggatgaagag gatgaggagg cagattgcct tgaatatatt gacaatactg
     3301 ataagataat atatctttta tatagaagat atcgccgtat gtaaggattt cagggggcaa
     3361 ggcataggca gcgcgcttat caatatatct atagaatggg caaagcataa aaacttgcat
     3421 ggactaatgc ttgaaaccca ggacaataac cttatagctt gtaaattcta tcataattgt
     3481 ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa caactttgaa
     3541 aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt ggagttcgtc
     3601 ttg
//
LOCUS       DAZKOW010000158         3528 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000158 DAZKOW010000000
VERSION     DAZKOW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.162"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="ACG912_002772"
     CDS             complement(<1..2232)
                     /locus_tag="ACG912_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFQ0514033.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACG912_002773"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACG912_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          997 a          778 c          551 g         1202 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaa
//
LOCUS       DAZKOW010000159         3489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000159 DAZKOW010000000
VERSION     DAZKOW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.163"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACG912_002774"
     CDS             complement(<1..182)
                     /locus_tag="ACG912_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFQ0514034.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACG912_002775"
     CDS             517..951
                     /locus_tag="ACG912_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFQ0514035.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACG912_002776"
     CDS             974..1294
                     /locus_tag="ACG912_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514036.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACG912_002777"
     CDS             1435..1626
                     /locus_tag="ACG912_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514037.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACG912_002778"
     CDS             complement(1697..1954)
                     /locus_tag="ACG912_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFQ0514038.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACG912_002779"
     CDS             2112..2375
                     /locus_tag="ACG912_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514039.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACG912_002780"
     CDS             2399..2587
                     /locus_tag="ACG912_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514040.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACG912_002781"
     CDS             complement(2670..2855)
                     /locus_tag="ACG912_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514041.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACG912_002782"
     CDS             3070..3441
                     /locus_tag="ACG912_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFQ0514042.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1302 a          498 c          699 g          990 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataattta
//
LOCUS       DAZKOW010000160         3336 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000160 DAZKOW010000000
VERSION     DAZKOW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.164"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            732..1064
                     /gene="traE"
                     /locus_tag="ACG912_002783"
     CDS             732..1064
                     /gene="traE"
                     /locus_tag="ACG912_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFQ0514043.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1683..2024
                     /locus_tag="ACG912_002784"
     CDS             1683..2024
                     /locus_tag="ACG912_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFQ0514044.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
     gene            complement(2261..>3336)
                     /locus_tag="ACG912_002785"
     CDS             complement(2261..>3336)
                     /locus_tag="ACG912_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFQ0514045.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         1085 a          491 c          677 g         1083 t
ORIGIN      
        1 aatagctatt ttaatagaat ttataagtta aaatattatg ataaaaatca caaacgtagc
       61 cttttacgtc ataagtgtta aaagaaataa ttaatttcaa atatagaaaa gggaacgtat
      121 gtttgcatac tccttttttt tgagttataa taatacgcga agagagataa tttcattatt
      181 taaacaagct aaaaatcttg atattacttc ttttcaggca atacgaatat tattcgctta
      241 cattttttgt ataaatttaa ttgtctgcta tgcttgtgat gaattgattt tcgttttgca
      301 cttttggatg ataaatttgg acaattgaaa atacaaaaaa aagaaaggcg gtatgaactc
      361 ggaaggctgg taaccttcgt cagcgttcat acacggcatt aagatttggc ccttaatgcg
      421 ttaacctttc agtgttacgg tattacccgc aatggattta gtataacaga tattcaaaag
      481 tgataccact ttttttggct gtgttaaggt taaaaatacg tgatattacg atgaatctat
      541 gcctaagcat aatgttataa aaatgtccgt gtggctcttt gccgtatgac gaacgctgaa
      601 attagaggga acgcatgttt ggatgcgttc cctctttttt gtttgtcatg aacgaaaatc
      661 aattctctat ttttgaaaaa taacaagaaa aacttgttaa aaatatttaa agaaattggg
      721 tggtttattt tatgtcatat acaggaattt tgagtctcaa agatatttgt cactacggta
      781 aacggtgtac cgctacagag aaaatcacga agaaactatc aactggccaa agtaaaaccg
      841 ttgtgcagtg taagaaatat ataatccaga aagacaaagt ctcagaggaa atgatttatt
      901 acattgggaa acaaaagcaa ataattttaa aagatcctat ccccttaaaa gagctatatc
      961 caacaatcaa acatgtttat gaccaaaacg gtgtactgat tggtcgaaga aaaaatggag
     1021 tcttgcgttg taccgcaaaa gggatggggc gtttgatcgg ctgaattgat cattctcata
     1081 acttgtattt tatcgttttt cgcttgcttg aaaatattgt cgagacttct aaaggaactt
     1141 agagtcacta gaaaaataag gagtagactt gatctatcaa caattccctt cggggagtga
     1201 cggggtaggt tttaacgatt ggtaacgtaa aaaattggtc aatcgagtaa atgcgcttgt
     1261 ttggatagtg gtgaaatgaa tttgtgtaaa taataagcgc agagtaaaca agagggaatt
     1321 tagaagttag acaagttaga atagcgtcta gattaatcat gagcagctac taagggatct
     1381 agactttgac gcaaactcat ggaataagga gtaggtcttc tcgtaatggg gaggacgcac
     1441 aagaaattgt gtcgtgtgct gcagtacatg tagatacttg cgaggacgtt gattattgga
     1501 tcaatcgagc atttaccgct gtctactagg gtttctcgtt tcggacgcca taaacgagtc
     1561 gaaatttttt atgtattttt aagtaaacga aaaacgataa aatatgaaca agtcctgaat
     1621 aaagtgaaaa aattaatata aagcatagag aaaatgaaat ttacgtaatg gaggaatgac
     1681 aaatgaaaaa ttatgatccg aatatacgat tgggcacaca tactattaag gtttctttcc
     1741 aacgttggga ttacaaagga tttgtgacgt ttagaagggg cggaaattgt aaaggattag
     1801 atgttttagc attagacgag gatgatttat acgatcaaac attgactgat aatccaattg
     1861 gattcggctt gctacctgaa gatgatgaag gtaatgaatg gtttaaaatg acgcttatga
     1921 atgataatgg agacgaatta tctgttgaag atacttggtc atatttgagt gattatattg
     1981 tcagctttga aatcattgaa tttgtagcgg ataaagaaga ataaaaactg gaggaataaa
     2041 ttttacagat aaaggcttag aaggagtcac gacgattaat aaaaaggtag tattacctta
     2101 ggcattgagt aaggaattta tttagtctaa tggtgataag tcaagatgaa atttaagatt
     2161 ttctttattg agtaatatgt tttgaagcgc acgaaactag acccgatcaa aatcaaattt
     2221 ttttcgggtt gattcaagga tttgcgtcaa taacggattg ttatttctct atcacactcc
     2281 ttggtggcgt atagagttgg tgtttacgta gtagcgtatc caccagacgc gtaaattttc
     2341 ttgcggttag gacgagtgca cgtttgtgtt gatgctttgg aacctcctta tatttacttt
     2401 gataaaaagc tttgtattca ggaagataat tttttacaga gttggcagct tcaactaagt
     2461 agtatctaaa atatcgattt ccttgttttg tcagaggtgt attttgcgac tgatagtttc
     2521 cagattggtt cactttccaa cttaatccag cgtattttgc caattttgtc tgatcttcaa
     2581 atctttcgat ttgaccaatt tcagcgatta aaccggcagc atagacttta ccaacgcctg
     2641 gaatactggt taagcattga tattctggaa tgacaacaat tacttgttcg atggctttat
     2701 ctaattcttt gatatttttt tcaagtgctc gtatttctcg tacgagaaca cttaaaacaa
     2761 cattaattga ttcttgtgca agagcaccta ggcggtaact catggtaatt gcacgttgaa
     2821 ttgctttagc gattttttct ggttgcttga aacggccttt tcctttttct tgaagtaaat
     2881 cacagaaagc ttctaaagga agctcagcaa gttcatccaa tgtgaaatct tccgtcatga
     2941 gtgaaatgag ggttgcacta aaaagactcg tcgattcgtc tcgcatttct cgagcaagcg
     3001 tattacattt gtacgttaga ttttcaagaa aatgttgctt tgtacgaatc aattgcttaa
     3061 taagttgata gcgagtacgt gttaaacgct gtaaggccat gtatttctct tctttgatag
     3121 gggaagttgt gaatcgttga atacgcaaga aatcagcgat tcttaatgca tcgtttcgat
     3181 cagttttatt ctcatcaaac atgcgactaa attgtttcac ccgaaaagga ttttcaatcg
     3241 ttacaagtgt gttgagcttc tttagtttct catcttcatg aaaaaacatg gaaggatgga
     3301 agctgtacat ggacgttgac tccatgccaa tcacaa
//
LOCUS       DAZKOW010000161         3221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000161 DAZKOW010000000
VERSION     DAZKOW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.166"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..198
                     /locus_tag="ACG912_002786"
     CDS             <1..198
                     /locus_tag="ACG912_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514046.1"
                     /translation="ELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADV
                     LGVKFVQAFILPDGNEIKIGN"
     gene            214..1056
                     /locus_tag="ACG912_002787"
     CDS             214..1056
                     /locus_tag="ACG912_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514047.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1119..2921
                     /locus_tag="ACG912_002788"
     CDS             1119..2921
                     /locus_tag="ACG912_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFQ0514048.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(2980..>3221)
                     /locus_tag="ACG912_002789"
     CDS             complement(2980..>3221)
                     /locus_tag="ACG912_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFQ0514049.1"
                     /translation="PPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSV
                     KGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1154 a          446 c          654 g          967 t
ORIGIN      
        1 gaactgtgtg agcaaatgaa tataagtgtt tccgaacttg ctagacgtat tggacagact
       61 ccacagaatt tcaataagaa attacaacga gaaacggtaa ccttagatga gttgaaagcg
      121 attgctgatg tgctaggtgt caagtttgtg caggctttta tattgcctga tggcaatgaa
      181 ataaagatag gtaattagga ggtgtaattt gtattgaaaa tcataacaat aaatgatgtt
      241 gagtatgctg tatttgctgc taatgaaggt acttccaaac cacaacctca tatcatagaa
      301 acgaaatctg gtactattcc agaaggaaaa caactgagtt tattaaaaga atatcttaag
      361 cagaatgata tttccccaat aaaaggagca acaacatatt ggtgcattga caaggttctt
      421 aagcttgatt caagtaaaga aaaaactata agtgaaacaa tccataaaca aaaatactta
      481 tctttaactg aagaaaatat tgagaaacag cataagtttg taggtgcaag ctctaattat
      541 gggaaagaag gtctaataat acatgatgtt ttgaatgcat ttcctcttca taatgattta
      601 aatactattg cgatgaagat tgcggtaatt gatgtaacaa attcaacaca tctttctcaa
      661 tataagagca ggttgtctct atatgatttg gctaaagtaa ttctggagat tccaaatttt
      721 gatgatcggt tggcaaaagg agatcctcag ttgataaata ttatcgcaag gaacattggt
      781 gcagttaata tgttctcatt tgcatccaag tactgtacat atcataatgt ggaagtgtgt
      841 gggagagatg attactccat ctttgatggt atcgttaaaa atactcttcc acactatatc
      901 caaggactaa ctacgaataa gattgatacg tggcgaagaa gttttgatta cgaggcattt
      961 aacgaatgtg ttggaaaact acttgatgaa aataatatac atattccttt tagaagaaga
     1021 aagttagatc acttcttgtg gtatgcgaat aggtagaata taagaaaaag gcatcgtaaa
     1081 agtgattctg gataaaatta atgggggagg aaataatagt gggattattt gattttctaa
     1141 agaaaaaaca aggaaaggtt agtaaacacg ttgattatca actggcttct agtctatttc
     1201 gtactggtaa agtttttgct gaaaaattgg aagtggacat atcaaatgta gatgagtttc
     1261 gtaaaacttt tatcgctttt gatgtggaaa caactggcct aaattcttct tcagatagaa
     1321 ttgtagagat tggtgcggtg ctatttaaag aaggagttcc aattcaatca ttcggaactc
     1381 ttgtcaatcc aaaagttaga attgcagcct cagcttcggc aataaataat ataactaatg
     1441 aaatgctaaa ctctgctcca aatgagcagg aagtttaccc ccagttggta gaatttttag
     1501 gagatgcact agatggaaaa atcactatgt gtgcacacaa tgcaagtttc gattttggat
     1561 ttttaagtaa cacattttct agacttgggt atgatgctaa aattaagtat gttgatactc
     1621 tgagtctctc tagaaaacat ataaaaggca ttgtaaatca taagcaagag actgtagcgg
     1681 aatatttcgg attaataaat gacactgccc atagagcgga atcagatgca gatatatgcg
     1741 gaaagatttt ttggggtgtt ttagatatta ttgaggagtc gttggaagaa caaaaaaagc
     1801 aaatagaaaa gatgactcca gacataaaag aacttgaagc ttgtgctttt attcaagata
     1861 ttattgagaa aaaaggatgc gacaccagtt ggatcaggta tcgaaaaaac agtaataatt
     1921 atgtcgatgt aacttgtcta tatacattct taaagtttaa gtttgctaag aagggaaatt
     1981 acatcattat tagaaaagat gctacaaatg gaattgattt acctatagaa ccttgtactg
     2041 taagtgaagg cggtacaaca aatgtgcgag tctattttcg tagccccttt gacttaacgc
     2101 ctctttcaaa ttatattttc agtgcttata gcgattgtta taattctatg aatgaatata
     2161 tgagtgttag taattacact aaaaaagaag cagagcaaag ccttagtatg ctgaaatcta
     2221 tttcaaaatc agcgatgaaa gagttattat tcgatgcaaa aaatcgtgga tatgatgaga
     2281 ctgaagttaa cctaaatatt gaaaaagtga tatctagaga agatgtaatt attaacgggt
     2341 tgcataatcg agtttcatta aacaagatac gaaatatagg gaattgggag aaaggatttg
     2401 atgcaggtta tccatattgg gaacgtggag aaactgctag gaaaaatggc aaagtagatg
     2461 aagctattgc actttttgat aaagctcgat ataacggcta tgatgctcct gcgctatatg
     2521 attcttatgc taaagcttat cgaaaaatta aggattacga taatgaaatt ctcattcttg
     2581 atgaaggaat tatgcgtaaa acacggcacg atgttggtac tttagaggct cgtagggata
     2641 aggcaattaa gctattattt gctaaacaag aggcagagcg gatagctaag gaaaaatctg
     2701 attttctaaa agcgaacaaa aaagaggatg cgattagtga gcctagcaag aagcgtggaa
     2761 gacagattat tcaaatgact gatgatggaa caataataaa agaattcgat actatagctt
     2821 ctgctgtaaa agaagttggc gttaactcaa aaagtattag ggatgctgca aatggagttc
     2881 aaaaacatgc tggaggatac tactggaaat acaaagatta gcagataatc taagataacc
     2941 taggatcttc ttaatagata cccgtggtgc tagttaattt taatatccta aagaatatct
     3001 caaagttcct tcaaaacgtc gttgtgctcg acttaataga acactgtaag ccagtaatat
     3061 gctttcaaat ctactctcta gccctttaac agaacgtgag ttgaaatttt gacaacctaa
     3121 tttttctaag gaagaaaaaa cagtcccaac tgtttttctt tgttttccaa gtaagctgtg
     3181 gtctagtttt tctgatttat ccatgttttt acgtggcgga a
//
LOCUS       DAZKOW010000162         3221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000162 DAZKOW010000000
VERSION     DAZKOW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.167"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(111..284)
                     /locus_tag="ACG912_002790"
                     /pseudo
     CDS             complement(111..284)
                     /locus_tag="ACG912_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(838..924)
                     /locus_tag="ACG912_002791"
                     /pseudo
     CDS             complement(838..924)
                     /locus_tag="ACG912_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2090..3130
                     /locus_tag="ACG912_002792"
     CDS             2090..3130
                     /locus_tag="ACG912_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFQ0514050.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT         1197 a          448 c          462 g         1114 t
ORIGIN      
        1 ggtattttta ttatatatag atttaacata tgtcggtcgg ttgattgata tagctaaagt
       61 tataactcaa ccgaagccga ttcatctaca cgaataaagt tgcgagtgtt ctaggctaat
      121 tcataaaaat tgttatgtgt tccatatagc aatactggaa taactttatt ttcttgaatc
      181 tcataagcaa ctctatacac ggtgaccgca tacttaaatc cattagacca attacctgat
      241 aaacctccag ttttttgctc ttacttaaat ggatcaatgg caatttcatc acaaacctat
      301 agaggatgag tgacttctgc ctaattacgt taaaaaatat tgaaagtaac tctatgtagc
      361 aaaaaatatc tagaatttca tttcctcaaa actgatcgat attgtatcta aaaatatata
      421 ctacctaaaa taggtagtat atattaaaat atgtgctttt gttagaaaaa tcaatacata
      481 aattatcata tctgtatatg tcatttaggt atttcagtta tgttataata gttttgctgt
      541 ctgctacccc ggcctaatct ggcaagggga ggaggagata taatgaaata cctttttgat
      601 tttgtttttg caatcaagcc acttgcagat gtagtgtgca gttacctctg caaatggctt
      661 gattgcaaaa ttgccaaaaa agacagtaag cctacccgtt aattctataa tatagaaacg
      721 taaaaaaaac cccatcagcc tgcacgctga tggggtttgg tgatataatg aaatacctct
      781 ttgttactca taattatacc ataattagtc acgaatgcaa ccaatagaaa aaactactta
      841 gaacggtaga tcatcgctaa taacaatgca ttgtccacca aatggatttt gtgaagcatt
      901 aaatacattg ttattaattg tcgctgcttt taactaggcc tctccccttc tattataaaa
      961 gaagatatat atcatttctt aattacctac taccttaaac caaaatttaa gtactaccta
     1021 atttaagtag tacttaaaca atctaaaaaa tagttatccc aattgttatg cttttataat
     1081 acgattattt gtaacaaaat gaatattata taattaaaca ctaccatatt taggtagtgt
     1141 ttaattatta gcaaaacaaa aaatatttat tctattctct taacttcaac taagttatgc
     1201 tgcacacctt tatataaatc atatgcaact cattagctag acaaactaca agcatgtagt
     1261 tttatctgtt aattatgtac tacctaaata aagtagttta taattagcaa gtatatatat
     1321 ttaaaaattt aagattaatt tttttatgac ttgtacaaat ttatctgata ttctcttatt
     1381 aagaactacc taatttgggt agttcttatt ttttacattt taagtagtat ttaattgcaa
     1441 tttggtagta tttttttatt atcttattct tagaacggta gtacttaata taaatatagg
     1501 tagtatctaa tttaagtatt aatggtagta tctaaattaa caggtagtat gtaactactt
     1561 taaagtaatt tttttcagat taacacagta gtatcttatt aaataagtta attttaatgg
     1621 tagtacataa ttattatata tatctgacat tggtagtact tattgtattt tttagtagta
     1681 tataatctat tgttttctag cctaaagcct tgtcttataa gaataatata atatatacta
     1741 ctgaaataag gtagtatata ttactagtaa tggtagtatg ttttatatgc ataaggtagt
     1801 tcttaattta ttttttgttt tgacactggt agtacttaat tatctttaat tttaaaacta
     1861 aattttattc atacgttttt ctggtagtat ttaattataa ataatattta tttaaagtag
     1921 ttcttaatga ctttaaaggt agtacataat tcattttatt tctgatttta ggtagtattt
     1981 aattaaaaaa cagttttaat gcgtttgcat tttttattta tctttgctat tttcgaaaag
     2041 agtaggtaat tttacataaa taaaacaacg tgaaagtaga ggatctacaa tgactgattt
     2101 taaatttttt aaggcggata gagtttacaa cgaattattt tatcaatttc caaaagtctt
     2161 tattgtttct gacgaataca aaaaaatgaa agattcaact aagattgcct atatgctttt
     2221 aaaagcaaga ttagagatcg caatcagcaa acggcaaatc gatgaagaag gtaatgttta
     2281 ttttacttat acgacaaatg aactatgtag agtattaaac tgccaaaaac aaaaagcgat
     2341 agcaatcaaa aaagagttgg aatcctttgg tttattatta caaaagcaga tgggatttaa
     2401 caaacagtta gggaaaaata atcctaatag actatatcta gcagaattaa aagtctcaga
     2461 aaatgatatc tacttactcg aaaaatttga tagagagaat agggaaaacg ttgataaatc
     2521 agagggtatg aaaatcatac ccaccctcga cgaaaaatca gacgctgaat cccttggggc
     2581 tcaagagggt atgaaaatca taccgtgcca aaacgttgat aaatcagagg gtatgaaaat
     2641 cataccagaa cttaataata atatattaga cactaataga cacaatatag acactgaaaa
     2701 agaccgccta caagatcaat tgttgttaga caattttgag acaattatga caaacgacag
     2761 cattgctacg tttgtccctg aacgagtatt aaatttgata aaaacatttt cttcaagtta
     2821 cagtgaagct caaaaaaccg tccagactat tcataatgca aagaaaaaag ctgaaataga
     2881 aagtgatatt tcgatagttt ttgaagaact cgatagttat tatgtcaatg cagaacaaga
     2941 attatacacg acactgttaa aagcctatca aaaattaaaa accgaaaaag tcgaaaatat
     3001 ccagaacctg atttttgtct atgtaaaaaa ttggtttatc gaaaaaccaa tagctgctaa
     3061 agtatcaagt gaaaaacgtt tgaattatgc aagctcccca agcactatta cgaaagactg
     3121 gttagagtga aactttactg acaaagtgat tgttgcttca tggagtaaca ttaaaattaa
     3181 aaatttttta ttattctaat tatattacga atattacaaa a
//
LOCUS       DAZKOW010000163         3207 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000163 DAZKOW010000000
VERSION     DAZKOW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.168"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..643
                     /locus_tag="ACG912_002793"
     CDS             <1..643
                     /locus_tag="ACG912_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFQ0514051.1"
                     /translation="KNTMINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRV
                     KEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSP
                     SRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQN
                     ISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            633..1373
                     /locus_tag="ACG912_002794"
     CDS             633..1373
                     /locus_tag="ACG912_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFQ0514052.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1370..>3207
                     /locus_tag="ACG912_002795"
     CDS             1370..>3207
                     /locus_tag="ACG912_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFQ0514053.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1203 a          390 c          623 g          991 t
ORIGIN      
        1 aaaaaatacc atgattaata catggaataa cattaaggac tctttctgga atattgttac
       61 tggaattgtt aattctgctg aaaatgcatg gacaaatcta aaaaatggtg tttcaaatgc
      121 tattaatcgg gtaaaagaaa tctttaattc gttaagggaa gtcaatttat ttgaaattgg
      181 caagaacatc attgatggac ttataaatgg tgtaaaagaa aagtggaatg ctttgaaaga
      241 tactatcaaa ggtattgcag gtaatatcaa aaattggatt aaaggagctt tagatattca
      301 ttctccatca agatggatgc gtgatatggt tggtaaaaat attgttcaag gtattatcgt
      361 tggtattgat aaagagcaaa gtaagttaga tcaaaccatg acaaatttag taaaaacacc
      421 gtctgttcaa cctgtagtaa atggaataag tcacctacca attacacaaa caaagcaaaa
      481 tatatctgct agtgaaacaa aagaaattca tttacattta aatgtttatg gagatttacc
      541 tgattcaatg gttcggaaga ttaccacaaa aattaaaaca gaattgacaa gacaaatgaa
      601 acgagatgct ggtgcagtag gaggaacatt atatgcaact taagagagga caatttttta
      661 ttaatcaaca ttattcttct gaatttaatg tgtatattca aaacaggcct gcttctgttt
      721 cagctagtcg agtaattgaa ttgagagagc gagaaggaaa tgattctatc attattgata
      781 aaggctatta caaaaatgtg actagaaaga ttgagtgtta ctataaagca ccttcaattg
      841 atatggtgca ggagtgggaa gatcgaatca ctgaatggtt agatatgtcg tcttatagtg
      901 attttattct ttactatgat gaacaatata tttatcaagc tgtagtaata gaaggacctg
      961 aatttaaagg aacaagaaaa acaggaaata ttgtcccttt tgaatttaca gtgagtattc
     1021 gaccatttaa ggaaaattac aatggtagat ttactattcg acaaacagaa acttttgaaa
     1081 tttataaccc agagaagtat tcttcaaaac cgcttattaa attgagtggt tctggagatg
     1141 cttcttttta tattaataag gataaatatg atttgaaatc gttggacaga gaattattta
     1201 tagattctaa attagaagag gcttatcgaa aattagatgg taatttagaa catcaggatc
     1261 aggtcacctt atttttagat tttccatttt tatatcctgg aaaaaatgaa attaaatgga
     1321 cgaaaaatat tcattcattt gaaataatgc ctaggtggtg gagaaaagta tgaaaccaag
     1381 aatatatagt cctactgaaa cagatttctc aacgaatggc ttaggaattt taaaggattg
     1441 tacaagatgt ggaatatatg aagtagctaa tggaaaatat gaattagagt tggattatcc
     1501 tttaggaact agatttgatg aatattttga aaatgactat caaataaaag caaagccaaa
     1561 cgatcaagaa gagtatcata tcttttttat tgatgataaa gatatagata cttttttaaa
     1621 tacagtaact atttatgctc agagtcgtac aaatcgactt ggaagacggg tagtcactct
     1681 tgtggaagtg gactctaaaa ctggtcaaga agcgatatca attattgaaa ctaaaatgga
     1741 taaaaaatct gacatacgac tttattctga tattacggct gtttctagca caatctttga
     1801 agcgagaaat gttttaaatt gtattgctgg tgaacaagga tcattgcttc aatattgggg
     1861 cggagaaatt aaacgtgaac catttaagct ttctttgtta aagcgaagag gtcgcaataa
     1921 tgttggaacg attcggtatg gaaaagatat gtctggtttg aaggtcaaat tagattggac
     1981 aggtataaaa acaagaatta ttccatatgc tgatcctcaa agtgaagtag gtacgactaa
     2041 tcgaatttat ggttcgccag tagatagtgc ctatattaat aactatcctg atgtctatac
     2101 agaacatgtt cagtttacag aagaacaagg agtaaaagac attaagagct taaataaaat
     2161 agctaaaaat tactttaaaa cgatcaatcc tggctgtgat aagcctaaag tttctattac
     2221 ggttgaattt gataagttga ctgatagtga agaagcgaaa gaatttgcga agattagaaa
     2281 ttatggtctg tttgatacat ttaaaatata tcataaaaaa tataatattt atcttgaatc
     2341 aaaggttagt ggactacaat acgattcttt atctgaaaaa actttgaaat tagaagcagg
     2401 agacattcaa gttgcttttt atcagcaaca agctgtaact attcaggata aattaaaaga
     2461 ttatgcaacg aataattata tgagtgattt taatgattat gtttcttcaa tgattacagg
     2521 tcaaggcagt gcaggaggat atgtagtttt atggccaaaa gaaaaacctt ccaacatctt
     2581 tattatggat aatccagatt tagaaaaggc aagagaagta ctaagaatga ataaaaatgg
     2641 gattgctttt tctaagaaag gatggcgggg gccttttaat tcagcttgga cattagatag
     2701 tatatttaat gccaatttta tacaaactgg attaatcaaa gcagatatct ttcaaaattc
     2761 ttttaacaag actggagatg tattgaaatt agttaatgga ctactccaaa tttggaacaa
     2821 caaaaagaaa ataatggagt taactaaaaa aggaatggaa ttttggaatt ctaagggttc
     2881 aattggaacg attggaacaa ctgattctgt tggtaatcct tttcctgggg cttctactcc
     2941 aacccctatt gaagataatt ctttagttat tcgtacaaat ggagacggca aatatatttt
     3001 gatttctcct aaagtaggaa aaggattagt tttattagga aatggtaaag caatttattt
     3061 tggagactta gatgtacaag gaaaacttac agttaaagga aaagaaataa ttcctggaca
     3121 aggtggtggt ccatcgggag gtggagaatc tactggtggg tatcctagtg aattaaaaac
     3181 agatgcagaa aagagagctt ggagaat
//
LOCUS       DAZKOW010000164         3201 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000164 DAZKOW010000000
VERSION     DAZKOW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.169"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            301..585
                     /locus_tag="ACG912_002796"
     CDS             301..585
                     /locus_tag="ACG912_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514054.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="ACG912_002797"
     CDS             588..1004
                     /locus_tag="ACG912_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514055.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="ACG912_002798"
     CDS             complement(1141..1860)
                     /locus_tag="ACG912_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFQ0514056.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2523..2609
                     /locus_tag="ACG912_002799"
     CDS             2523..2609
                     /locus_tag="ACG912_002799"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFQ0514057.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2912..>3201)
                     /locus_tag="ACG912_002800"
     CDS             complement(2912..>3201)
                     /locus_tag="ACG912_002800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514058.1"
                     /translation="EYRRQRSDFISRKIVRLEKEKMEKFPYEYFQNLEKLEQAIQAIK
                     FDLTDEKLLEYKNMTPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1066 a          597 c          499 g         1039 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaaa ctatgtcctt cgttctaaag cgccttacag
     2401 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
     2461 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
     2521 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
     2581 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
     2641 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
     2701 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
     2761 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
     2821 gatttaagac gttttattta gtttttggtg tattttcatt ggtccaagct aaaaaactct
     2881 tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga aattcgctcc
     2941 atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc attaattttt
     3001 ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt tcgtctgtta
     3061 aatcgaactt gattgcttga attgcttgct ctaatttttc taagttttga aaatattcgt
     3121 aaggaaattt ttccattttt tctttctcca gtctcacaat ttttctagaa ataaaatcac
     3181 ttctttgccg tctatactct c
//
LOCUS       DAZKOW010000165         3073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000165 DAZKOW010000000
VERSION     DAZKOW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.170"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            289..1104
                     /locus_tag="ACG912_002801"
     CDS             289..1104
                     /locus_tag="ACG912_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514059.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            1230..1487
                     /locus_tag="ACG912_002802"
     CDS             1230..1487
                     /locus_tag="ACG912_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514060.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(1739..2311)
                     /locus_tag="ACG912_002803"
     CDS             complement(1739..2311)
                     /locus_tag="ACG912_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0514061.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG912_002804"
     CDS             complement(2327..2932)
                     /gene="fic"
                     /locus_tag="ACG912_002804"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFQ0514062.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT         1020 a          539 c          473 g         1041 t
ORIGIN      
        1 aaaaataaga aattcaaaaa agaaaaaact ttccttaaaa acaaaaaata ggagtattca
       61 gtttcttaat agttttgtaa tagttgtatg tagaagtttt cttaatagtt tttgtaataa
      121 tttattaatc gtagtttata taacttattt gtaagtgact agagaattaa ataaaaaata
      181 attactatct agtacgaaat taattacata ggataatata ttaaggtgct ggactacaag
      241 taaaaaatac tgtatgatca tgacagattc aagaaaggag caataagatt ggttaagata
      301 gcaggacttt ctaaaggtgg aaaaaccgtt tcacaacttt ataaacaaaa ctacagagac
      361 tggagacagt tcagaaaaaa cattaaggat tcttacttta ttctaccaac agaacttgaa
      421 aaatatttac cgaataacaa agctattaat ttatatctgt attactgttt taaggcaaag
      481 aatgaaggcg gggattcctg gcattcggtc aataccatag cagagtctct aggagtgaca
      541 actaaaacta taaacacctg gaataaaacc ttagttgatt taggtatgat tgcacgtata
      601 gacgataacc atctcagtaa atcgacaatt ttacttcctc tctctagtta ctataaatta
      661 gcccttgata gttctttaaa ggatgttacc gagagcactg tccatgaaat tgatggcaac
      721 ttggttgcag tttatcattt gtttgaatgg agaaaaaatg aggctgatga aaattataac
      781 gttccttata acacactctg ttgtgttttt aaaagaaaaa cacaaaaaga tactatctat
      841 aaatttatct tggtccaaga tgagaaaata agtaatttcg aattaagcac tccttctagt
      901 aagatttatg acgatgcata tagatttgaa tgtcaagatc tagaagccct cctttccgca
      961 aataatctaa acaatataga acatgaaaac tttgttgtaa acactagatt taatttagtt
     1021 tctgaaaagg actcagattt gttagatttg ctgatagagc ttactaaaaa tctagataag
     1081 aaagaaaaca ttactgtact ataactagca tcggtccatc attaagtttg gactattaga
     1141 acacttgcta ttacagaaaa aggtatgcta gccacgctta ctatttattg gtatcaaaca
     1201 agtatcttaa tttgaaagga agatgattta tggctaattt atatatagag aacaaaggag
     1261 acttgaatgc cattattact ttaatcgtag ttatggtaaa atcaataggt aatatcacaa
     1321 taggaatgct gtttattata ttagcagcag tgctgatttt tatgatagtt atttggttct
     1381 taaatgaaag atctaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
     1441 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
     1501 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
     1561 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
     1621 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
     1681 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
     1741 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
     1801 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1861 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1921 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1981 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     2041 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     2101 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     2161 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     2221 tcttctctac gccgttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     2281 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     2341 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     2401 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     2461 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     2521 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     2581 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     2641 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     2701 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     2761 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     2821 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2881 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2941 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     3001 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     3061 ttgcacagtc tag
//
LOCUS       DAZKOW010000166         3038 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000166 DAZKOW010000000
VERSION     DAZKOW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.171"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG912_002805"
     CDS             complement(26..961)
                     /gene="murB"
                     /locus_tag="ACG912_002805"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFQ0514063.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1034..1954)
                     /locus_tag="ACG912_002806"
     CDS             complement(1034..1954)
                     /locus_tag="ACG912_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0514064.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2067..2819
                     /locus_tag="ACG912_002807"
     CDS             2067..2819
                     /locus_tag="ACG912_002807"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFQ0514065.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT          856 a          612 c          526 g         1044 t
ORIGIN      
        1 tacttatttt aagttccatt acttattaat tggctaaact tctgcttgtt tctgatattg
       61 cttgtttttc tccaatgatc cgcacttctg tttctaaaga cacatcaaat ttttctttga
      121 ttacttcttg gatgtgcgca atcaattcaa tataatctgt cgctgtcgca tgatcgatat
      181 tgacgatgaa tccagcatgt ttttcagaga cttgtgcacc gccccatttc aggccttgca
      241 atcctgcttg ttggatcagc tgacctgtat agtgtcctac tgggcgctta aagacacttc
      301 cgcatgaagg gtattctaat ggctgttttg attgacgcaa ttcggtcaac tcatccattc
      361 ttgctttgat tgcttcataa tcccctgttt gcaaactgaa tcgagcttcc aatacgatag
      421 cacgtaattc ttggacttta ctgtgacgat aagaaaatac catttcgtct tttgtcaaag
      481 ttttcaatgt tccatcttca agcaaaagat ccacttcagc aaaaacatct ttgatctctc
      541 cgccatacgc acctgcattc atataaaccg ctccaccgac acttcccggg atgccgcaag
      601 caaattcaaa tccagtcaat gattctgcca aagctgcata agtcgtatca atcagtgagg
      661 cacctgcttc cgcaatgacc atcgttcctt tgacatgaat ctgtttcatt tcagtcagca
      721 tgatgactac accgcgaatc ccgccatctt gaacgatcag attgcttgca tttcctaata
      781 ccatccaagg tatatcatgg attcggcaat aatcgatgat ttgctttact tcttcttgtt
      841 ttttcgggaa tgcaaggaca tcagctggtc cacctgtttt cgtaaacgta tagttcatca
      901 atggttcatc aaacaatata gtaatcgtgg ctaagctttc aactatttct tctttattca
      961 ctgaggattt tcctttcttt tctcaaacgt cctcattatt ctatcacgct taatacaatc
     1021 ttgccagtct tgttcaaagt tgatgacggt agtgatcttt aagcacttcc tcacataatt
     1081 catgacttct tacaacttct ttttgtttaa gcaattctgt ttccgattta cctaaacgga
     1141 tctgagaaaa gaaggcctct accattggct caaacccgcg tttgactagc gtgtttgtcc
     1201 agtcgccaaa ttctttcact ttttttccat cagcatcttg aatgacaagc tgggtcagat
     1261 cgtttacttc ataagtacca tttttacttg ttacttgata tttttctata ttagcgccac
     1321 ttgctaaatc catcgttaaa atagccgttt gattttgtgt ttctaattgt aaaatggcat
     1381 attccaaaaa ttcctttgtt tctcttatct ggtattttgt tctcaaaatg ggttcatcta
     1441 ataaatacac agctgtatca ataagatgta aaaacaaatc ataaaggacg aactctgtcg
     1501 tttcttttgc cgctattcga tttttttgca ggtggatcaa ccgcttatct gggatacgtt
     1561 tcaattcttc taccatagga gcaaagcgcc ggttgaaacc gatcatcaaa attatttggt
     1621 tcttatcagc taattcttgc aattcacgaa cttcatttag atggacactc agtggtttgt
     1681 caatataaac atggatacca ttttctagac attttttggc taattcataa tgtacttttg
     1741 ttgctgcgtg gatcatacat gcttcgattt tttcttctaa aagctcatcg atcgtttcat
     1801 atagatgttg gaacccgtac tctttttgaa ttctttcttt tgtttcttta tttcttgttg
     1861 caaaataaaa atcaagtgtt ccttgttttt tcgcataagt tggtaaatat gctttttctg
     1921 cgatattccc tacaccgatt acaccaatct tcatctactt tcctcctgga tcatttagtt
     1981 actttcatct tacaccattt tttactaaaa tgaggtacaa tagtatcgca aaggtaagga
     2041 aattaattcg aaggagtgtg cttttattga aatttatctc ttggaacgta aacgggcttc
     2101 gtgccatcgt aaataaaaat tttttagaag tttttcatga gttagacgcc gattttttct
     2161 gtctgcagga aacgaaactc caagcaggac aaattgattt agatcttcca ggttattatc
     2221 aatattggaa ttacgcggaa cgaaaaggct attccggcac agcgattttt gctaaaaaac
     2281 cagcattgaa tgccacttac ggaatgggta tcgatatcca tgatacagaa ggccgtctga
     2341 ttactttgga atattccgat ttctttcttg taacctgtta cacgccaaat tctcaaagcg
     2401 aattaaaacg tttagattac cgattagaat gggaagaagc cttttataat tatttggaaa
     2461 atctaaaaaa acaaaaacca gtcatcgtgt gtggggatct aaatgtcgct catcaaaaga
     2521 ttgatttgaa aaactggaaa acgaatcaaa aaaatgctgg attcactcca gaagaaagag
     2581 cggctctttc tcgactgttg gataacgggt ttatcgatac attccgttat ttttatccaa
     2641 cccaggaagg tgtctactct tggtggaact accgattcaa ttcaaggaaa aacaatgctg
     2701 gttggcggat cgattacttt ttgacgagca aagatttaga gccaggacta gctgatgcaa
     2761 agatccatac ggacattatg ggcagtgatc attgtccagt agaattagat ttgaaataaa
     2821 gaggttgtga aaaagtcgtt tagtttcgag tattaagaaa aattttgtga aaatggcttt
     2881 ccacattttg cataaatttg gcataatacc gagaaactgg cttttgaaca ctgtttatcg
     2941 agagattgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagttc
     3001 ctcatatttt tcgtttggtt gggcttatta ccgcagat
//
LOCUS       DAZKOW010000167         3017 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000167 DAZKOW010000000
VERSION     DAZKOW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.172"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            191..958
                     /locus_tag="ACG912_002808"
     CDS             191..958
                     /locus_tag="ACG912_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFQ0514066.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            948..2990
                     /locus_tag="ACG912_002809"
     CDS             948..2990
                     /locus_tag="ACG912_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFQ0514067.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT          972 a          483 c          609 g          953 t
ORIGIN      
        1 ttgtgaaaaa aggtgaaaac ttgtagctgg tggatttgtt ttttgcatat ttaattttgc
       61 tgaataaaat aattgaataa aattttttat tcagtccaag taaaagtgac aaaaacataa
      121 gagcaaatta ttcgtagata tgctactatg caaataaata gaaatgaaaa atgaaagagg
      181 aggagaagaa ttggaaaaga tgatcgaagt gaaacatcta agtaaaagct acggcagcaa
      241 actttctccc gtcaaagcct tagataatat ctcattcaca gtagaagaag gcgagtttgt
      301 tgggattatg ggcccaagtg gtgcaggaaa gacaacgctg atgaatatct tgtccacgat
      361 caatttgcct acgatgggca gtgtctcgat ccaaggaaaa gaaatcacaa aaatgaaaaa
      421 cagtgagctc agtgactttc gtcgaaaaaa actaggattc atttttcaag agttcaattt
      481 gatcgatacc ctaaatgcaa aagacaatat cctattgccc ctagcagttg aacggatgac
      541 taaagaggaa atggaaaaga gagtaagaca tgtagctcaa ttgttgaata tagaagaact
      601 gctcaaacgg tatcccgatg aactttctgt cggacagaga caacgaattg cagctgctag
      661 agcacttgtt gttcagccac aagtgatttt tgcagatgaa ccaactggct ctttggactc
      721 gaagtcggct acagaattgt tgaactactt gaagaccatg aatcaaaaag aaaaagcaac
      781 gatactgtta gtcacccatg atccatatac agcaagctac tgcgatagaa ttttatttat
      841 taaagatggt gtgatttttt ctgaagttgt cagaagaggg acaagaagag agttctttga
      901 gaaagtaata gacatgcagg cgacgattgg cggaggagga aaaatgaatg ctgtataaac
      961 tagccgttaa aagttttttt cggcagtcac gaggctatct tgtttatttt ttcagtttga
     1021 ctttatccgt aatgatctat tattccttca gcgcaatgac ctacgatcaa tctttagtca
     1081 gaagagcaag tcaagatact tcgatagatg gtggattgag ttttggtgga tttatcatta
     1141 ctgttgtttt gctttttttc gtattcagtg ccaaccgatt tttcttgaat agaaggcaaa
     1201 aggaaatagg catctatcag ctttttggta tgaataaact gcagatatca ggtatatatg
     1261 tgttagaaat catgataatt ggattgttcg cctgtatttc tgggatatta ctcggtatta
     1321 ttttttccaa actcttttca atgattttgg ttcgaatgat ggatatggat cttactagtc
     1381 cattttttat ctccattcca tcagtagtag atactttatt tgcttttttc atcatccttt
     1441 tagcagtatc tgtatattca atatggaaaa tatggcgcta tccaatgatt cgaagttttg
     1501 gagaaaaaga acaagcagat agttcaacaa tgcgcttcag gacaaggcac cgattactcg
     1561 cagtcatcgg gttactactt ttaacgagcg gatattttgg agctagtcat tttagggaaa
     1621 tcacttcatg gatgatcagt tatcattatc ttgatacatt agttttcttt tttccttttt
     1681 taattttttt cgtttgtgtg gtcggtactt atttgttttt ctgttataca tttcggttat
     1741 ttttgatcat attgagtaaa tggaagattt attactatgg tatcaatttt ttgataatgg
     1801 gtaacacgca agttcatttg ctcaaaagtt ggcgtacaaa ttctctgatt acagtcattc
     1861 tcggaatttc tttaatgaca attggcggca cggctagtat ctctactatt ttatcctacc
     1921 aggagaaagt ttcctccccg atggactatc agtttgatgt tgagacagag aaaaaaatca
     1981 agccgattct agcagaggaa gggcagaaga taactaaaga aatcaccttg cattataaag
     2041 tcattgggag tatctatagt caaaatctcg aagaacctat gtttcaaact gctaatctga
     2101 tcacggaaac acaatatcaa gccttcagaa aagttaatcc agatttgccg aacatttctt
     2161 tgaaatcacc tggtcatact gttttgcttg atcaaatgca aactcttttt cggaatattt
     2221 cattatatgg atctagtatt ggactgccag ataatcaaga attgaagatt caacaaatgc
     2281 ttccaagtgt attaggtgac gaaacaatgg tatatctaag tcctgtactg attgtagatg
     2341 ataccgtttt tgaaaaagca aaaggtgtgg attatcaagt agtcaatgta gatgtatcag
     2401 gtggaaataa tgaaaaaata gatgaacgac tgagcaatga cctgtcgata aagtggctca
     2461 atgctgttta ttattcttat gatatcgtaa atggaagtct acaaggagaa attagcacaa
     2521 agaaacttcc ggatgaccaa atgaaggaag cacaatttag ccaagaaagc tctcgtttga
     2581 atttctcttc cagctattcg aaattgcgtg cagttaatcg gcgaatcggt atcaatattt
     2641 ttgtcacact atttgtcggg atgatcgtga ttattgctac tggtagtatt ttgacagtcc
     2701 ggcaattagc agaagcagag gaagaaaaag aaaattatca cctattgact aagctgggga
     2761 ttccacaaag aaggatcaga cggatgatct tcgagcaaaa cgcacttacc ttctttccac
     2821 ccatgatatt agggatcact cacgcagtat ttgcaatcaa tgtcttcact cagtatataa
     2881 aaactgccga ttactggttg gcttatcttg tgagtggctt attgatcctt atctatcttt
     2941 tgctttatgt cattacgagt cagttatatt gtcggattat cgaggaatga tatacggaat
     3001 aagacaagat aataaat
//
LOCUS       DAZKOW010000168         2985 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000168 DAZKOW010000000
VERSION     DAZKOW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.173"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(415..699)
                     /locus_tag="ACG912_002810"
     CDS             complement(415..699)
                     /locus_tag="ACG912_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFQ0514068.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
     gene            complement(1218..1703)
                     /gene="vanZ-A"
                     /locus_tag="ACG912_002811"
     CDS             complement(1218..1703)
                     /gene="vanZ-A"
                     /locus_tag="ACG912_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0514069.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1856..2767)
                     /locus_tag="ACG912_002812"
     CDS             complement(1856..2767)
                     /locus_tag="ACG912_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0514070.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT          907 a          555 c          426 g         1097 t
ORIGIN      
        1 tctccaataa actatttttt atataagttt tacattcatc atgattcata caaactccac
       61 cttctataaa tgaatacaaa aaaagcaatc aaacgatttc cgattgattg cttaacaatt
      121 cttaaattca gtagcttaga tacttgaaaa ctctctgatt tccctatata atgatagtac
      181 ggttatatac cgtcttcaaa caaagttaat taaataactt cttacgaggg aagagttcat
      241 ctgactaact gataagcgtt ggtttggcaa tcttatcggg ctatgcattt ataaaatgtc
      301 gtcaaacatt ttataaatgt gtcatggctc ttttttcgtt tctattcagt tcgttgtttc
      361 gttatatcta gtataccgct tttaaaaaaa ataagcaacg atttcgtgca ttattcacac
      421 gaagtcattg cttttttctt cttccatttc taaatccaat gttacttgtt ctgattctgt
      481 ttctggttct ggttctgttg gctcatttgg gattaaatcc actactagcg ttgagttagt
      541 taactttgca atttgttcta gtgtttttat ggttggatct gattttcctg attctattcg
      601 tgaataattt gatctactca tttctaattc ttgggagaat ttttcttttg atttggtacc
      661 tctcatcttt tttaagcttt ctctaaatgc tagttccaag tttctccccc ctctttttgt
      721 gtaataaaat tcacgcttta tttaaattat agcttttaat atcttaataa acaagtgatt
      781 aatcacttgt gatttgtgat tattccaaaa gtaggtaatt tcaccaacct ttttcaaaag
      841 tttttatcga cgattattgg actggatcgt gtttcctaaa gcgattctaa gcgcatgttt
      901 aattttaaag tgggtaattc tctttaaaac gtcctacaaa gattcaaaaa aagcatggct
      961 gctagtgggg gtaccgtcag gaaaatgcgg atttacaacg ttaaggaaat ttattaaaga
     1021 gaccaattcc tcagcactaa aattgataat tgggctttct ttgttactat agtgttaggt
     1081 aagcttttta ctttatcacc ggttttcccc tcacttcaca cctacgggcg agtttcccca
     1141 catcccgttt tccatctaat ccaattcatt caatatcttt atgtgtatga aagatcagat
     1201 tgcttgattt agacatctta cacgtaattt attcttaaat gggtacggta aacgagcaat
     1261 aatacgagca aaagtatacc tacaaaaata ataactctgt ctaatttttt ttgattcata
     1321 tgcttattgc ttaaaccata taatttcagt ccaagaaagc ctccaacagt atttgtaatt
     1381 acatctgtta tgtctgtcgc tccaatagcg aagataaatt gaattatttc aaaagtaaga
     1441 cttaaaacca gtacaaaagc aaacttaggt aaaaatccga tttctttaaa attgacattc
     1501 aaaagcaagc caaatggaat aaagattata acattatcta tcatctctct gaaattccca
     1561 gtagcagtaa atggagtcaa gttaagactt ctttgatgat aattaaatac tgataaaata
     1621 ttgtattgta atttgaataa cactaaccag attagtgtca ctaaatataa agctagcaat
     1681 cctctagata atatttttcc caatataatt tcctctaatt cttttgttta agttgtatca
     1741 ttagtatagc aatgtcctag ttaacgtggg ttttaaatta aaaagctaac gttaccccaa
     1801 aagtgctagt acaataatgc tgttcattaa tttcgttggt ttcattccgc catccttacc
     1861 tccttgaatt agtatgtgtt gatccgggaa acactgtcac aattacaccg tctatattgt
     1921 ttcctgatat ctcataacga agattagtcg gcacatgaat ggtggtattt ttagtaacag
     1981 gataataaaa gatctcatat ttttccccat ttacactaac agaaatggtt ttttcttctt
     2041 ttaggtaatc catatattcc tcgagaacga aattcttttc tttcataatc gcactatgtg
     2101 gtaaaccaac atagcgaata tgccatggtt catattgaat tcctgttaac tctgttttgt
     2161 cctctggata acgtaaaatg aacccgtatt tccaagcatt ttcttctatc cactttcctt
     2221 caggggctcg ttccattttc gtcaagcttg atcctacatc tagtgataaa cctgaattat
     2281 gctcactata acctgctggt aaggcatact cagcccccat ttcttggtaa agcacacttt
     2341 gctcatcaaa gtctcgatag ccactattaa taataaaatg actaacgcca ccctttacag
     2401 catcattgac catctctgaa aatttttgtg ctatttcttt tgacatataa atattactat
     2461 caagcaaccc gtatccattt attaattcgt catgtttaga taaattcacg atatctgact
     2521 tcacactttc ttggcgaaca ggatatttac tattgattaa tagcagattt ccttgataaa
     2581 cctgttcttc tgtaattgtt ttctcttggg tattttcaga agtcccacta ttttctaaat
     2641 gttctttggg attttgatca taattttgaa attcgacttt ttcctgagaa aacagtgctt
     2701 cattaacgta gtcataacct aagtatatta agaataacaa taacaataaa aaaaacaact
     2761 tcttcatttt cagtctcctc cttaactata tagttggaag tatgaaatat ttctagaatc
     2821 ttccccttaa acggcacaat ctcgtttgag attgtgccgt tttgccccgt aaattacagt
     2881 caattatttc gccctactgg atttactatt atcaccaatg tagatgaatt ttcataagtg
     2941 atacccatgc tgaatttaac gaggctgaac tgcctgttga caatg
//
LOCUS       DAZKOW010000169         2929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000169 DAZKOW010000000
VERSION     DAZKOW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.174"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..981
                     /locus_tag="ACG912_002813"
     CDS             85..981
                     /locus_tag="ACG912_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0514071.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIVHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLERIIQLENEKE"
     gene            1073..1288
                     /locus_tag="ACG912_002814"
     CDS             1073..1288
                     /locus_tag="ACG912_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0514072.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            1306..1578
                     /locus_tag="ACG912_002815"
     CDS             1306..1578
                     /locus_tag="ACG912_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFQ0514073.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILTKEKVA"
     gene            1580..2443
                     /locus_tag="ACG912_002816"
     CDS             1580..2443
                     /locus_tag="ACG912_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFQ0514074.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAI
                     ISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT         1160 a          464 c          510 g          795 t
ORIGIN      
        1 gagggctttt atatattaat cacaaatcac ttatcacaaa tcacaagtga ttaatcacaa
       61 atcacttgtg atttgtgatt cttaatgata caatattact atacaaaaaa agaatggggc
      121 gtagttatgg agaaggaaaa actaaaaata cttgaagaat taagacgtat tttaaacaat
      181 aaaaatgaag caattattat cttgaataat tactttaaag gtggtgttgg aaagtccaaa
      241 ttatcgacta tgtttgctta cttgacagac aaattgaatt taaaagtttt aatgatcgat
      301 aaggacttac aggcaacatt gacaaaagac ttagcaaaaa cttttgaggt agaattgcca
      361 cgtgtcaatt tttatgaagg cttgaaaaat ggaaacttgg cttcttctat tgttcatttg
      421 actgataatt tagacttgat ccctggcacg tttgatttga tgttactgcc aaaattaact
      481 cgctcatgga cttttgaaaa tgaaagtaga ttgcttgcta ctcttttagc acctttaaaa
      541 agtgactatg atctcattat tattgatact gtaccaacgc caagcgttta tacaaataat
      601 gcaatcgtgg cgagtgatta cgttatgatc cctttacaag cagaagaaga aagtacaaac
      661 aacattcaaa actatatttc ctatttgatt gatttacaag aacaatttaa ccctggacta
      721 gatatgatcg gttttgttcc ttatttagtt gatacggaca gcgcaacgat aaaatcaaac
      781 ctggaagaac tgtacaagca acataaagaa gataacttgg ttttccaaaa tattatcaag
      841 cgaagtaata aagtaagtac ctggtctaaa aatggaatta cagaacacaa aggctatgac
      901 aaaaaagttt tatccatgta tgagaacgta ttttttgaaa tgcttgagcg aattattcaa
      961 ttagaaaacg aaaaagaata gaatcacaaa tcacaagtga ttaatcacaa atcacttgtg
     1021 atttgtgatt gttgatgata aaataagaat aagaagaaat agaaagaagt gagtgattgt
     1081 gggaaattta ggcgcacaaa aagcaaaacg aaatgataca ccaatcagtg caaaaaaaga
     1141 tataatggga gataagacgg ttcgtgttcg tgctgactta caccatatta taaaaattga
     1201 aacagcaaaa aatggcggaa acgtaaaaga agttatggat caagccttag aagaatatat
     1261 acggaaatat ttacctgata aactttaaaa aggagtgaaa atgaaatggc agttacgtat
     1321 gaaaaaacat ttgaaataga gatcattaac gaattatcgg caagcgttta taatcgagta
     1381 ttaaactatg ttttgaacca tgaactagat actaaaaata ctcgtttact agaagtgaat
     1441 cttttaaatc aattagaagt ggcacaagaa gttgatttat ttcaacaacc atttgaagaa
     1501 ttacaagcta ttcatgagta ttggcggtca atgaatcaat attcaaaaca aattttgaca
     1561 aaagagaaag tggcttaaca tggcgaatat tactgacttc accgaaaagc aatttgaaga
     1621 tcgtttagaa aagaatgttg aacgactaac taaaaataga ctagcggttg aatcgccaac
     1681 cgctttttta cttggtgggc aaccaggatc aggtaaaact agtttgcgat cggcaatttc
     1741 cgaagagaca caaggaaatg ttgttatcat tgataatgat acgttcaaac aacagcaccc
     1801 taattttgat gaactagtga aactttatga aaaagacgta gtaaaatacg ttacccctta
     1861 ttctaatcgc atgacagaag cgatcataag ccgtttgaga gataaagggt ataatttagt
     1921 gattgaaggt acaggacgaa caacagacgt tcctattcaa accgcaacaa tgcttcaagc
     1981 caaagattat gaaacaaaaa tgtatgtcat ggcagtacct aaaatcaact catatttagg
     2041 aacaattgaa cggtatgaaa ctatgtatgc agatgatcca atgacagcca gggcaacacc
     2101 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     2161 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     2221 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     2281 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     2341 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa atggaaagct tgcagccacc
     2401 tacaccacca atacccaaaa cacctaaact tccagggatt taaaccttaa aaaagtaaaa
     2461 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatttttta
     2521 aaagctgatc gagaaataaa gcacacaact gtattctttc ttttttcagt tggtacattt
     2581 tgcagtctgt aacgtgccat ttactgtttg ttaccttaac atatccagtc gaaaacacaa
     2641 gggtatataa acgagcaagc tcgcccttgt gttttctttg tgtctatgtt gcgttctcgt
     2701 cacagcaaac gacacgaaca cactggcgaa aatgaaccaa caaaacgaaa aaagaaagga
     2761 gcgtacactg taatttattt ctagctttta aaacatgagg aaaacagctt cacttcctca
     2821 aacgcctaac aaaaatggtt gtcaaaaaac taaaaatcaa aaaaagaaaa gaggaaaaga
     2881 aaatggaaaa aacaaaaaaa ttacaattag aagattttac agaaaatgg
//
LOCUS       DAZKOW010000170         2905 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000170 DAZKOW010000000
VERSION     DAZKOW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.175"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..624
                     /locus_tag="ACG912_002817"
     CDS             85..624
                     /locus_tag="ACG912_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514075.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     FIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="ACG912_002818"
     CDS             1644..2813
                     /locus_tag="ACG912_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFQ0514076.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT         1080 a          391 c          457 g          977 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taata
//
LOCUS       DAZKOW010000171         2872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000171 DAZKOW010000000
VERSION     DAZKOW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.176"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..529)
                     /locus_tag="ACG912_002819"
     CDS             complement(152..529)
                     /locus_tag="ACG912_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514077.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(829..1710)
                     /gene="galU"
                     /locus_tag="ACG912_002820"
     CDS             complement(829..1710)
                     /gene="galU"
                     /locus_tag="ACG912_002820"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFQ0514078.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(1753..2772)
                     /locus_tag="ACG912_002821"
     CDS             complement(1753..2772)
                     /locus_tag="ACG912_002821"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFQ0514079.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT          791 a          643 c          436 g         1002 t
ORIGIN      
        1 aaaggaatgg cttatcccga tacaacaagc attcctttaa aaaagattct tttatataag
       61 tgaaaaaagc ttttttccac caattaaata aatctctcat ctcaatagtt tatccattat
      121 tttatctttt tttgcgtata cctactaaat attaccgtct tcttctcgtt cttctacttt
      181 gttcgagaga atctgtacta gaaaaagtag ttgtatgtct accttttctg cctatccgag
      241 actttctttt catatatggg atataaatga ttagatacag tacagcaaaa aataaagcaa
      301 atacgcaagc ttcccaacta cctaacgcca attcaaccat caaggtagca accccaacgg
      361 atgctgcata ccagataaaa atcagaaacg tttctttttt tctgaccatg cgaaacatga
      421 taaatagact aataagcaat agtggcaaca caaatatcca attgcttcct tccatcagcc
      481 acttgagtat atcaagcagc gactgtgcaa cagcgactaa ttttcccata gtgaacacac
      541 accttttttt ataattaaaa aattcgcctt tttattctac tatgaatctt ctggcttggc
      601 tttcatttct cttatgtaat cattttcttt tatacaactt aaaaaaaaat taaaccatta
      661 gcataatatt ttctgaatct attcacgaaa gtctttttct atctttcgtt ttccacgact
      721 caactgatct agagtcaaaa aactaaaacg acagttgcgc aaatgacgca caagctgccg
      781 ctttaataac aaatagcctt tcattctcag caggccactt atttccagtt aactttctgt
      841 tcttagcttt ttactcaact ggataatata tgcacttaat gaagtcccaa tttccgaatg
      901 ttgaagtcca tattcaatat ttgtcttcat atatcctaac ttatcgccta cgtcgtatct
      961 ttttccttta aactctctcg catagaccgc ctctgtctgg ttaagagtct gaagtgcatc
     1021 cgttagctgt atctcgtttc ctgctcctgg ttcttgagtt tctaatatct caaatatttt
     1081 aggagtcagt agataacgtc cgataatggc taaattactt ggtgcttttc ccatttcagg
     1141 tttttctaca aaccctttta ctctgcaacg acccttttcg ctatctgttt taggatcaat
     1201 aattccgtat ttagatgttt cattttctgg tacacgcatg acggcaacag cggaagcttg
     1261 tgtttcttca taatccatca tcaattgttg agtcaatggg atatcatctt tcatcaaatc
     1321 atcccccagc atgacgacaa acggttcatt tcctataaat gcttttgctt gaagcacggc
     1381 atcccccagt cctcttggat gagattgacg gatgaagtga agattgactt gagtcgtttc
     1441 ttcaactaaa gcgagtaatt ctgttttttc cttttcccgc aaattgtttt ccaattcgat
     1501 atttgcatca aagtgatctt caatgggtct tttttctttt cccgttacga tcaagatgtc
     1561 ttcgattccc gaatccaatg cttcttccac aataaactgg atcgttggtt tatccacgat
     1621 tggaagcatt tcttttgcca tggcttttgt tgccggcaaa aaacgtgttc ctagcccagc
     1681 ggcaggaatg actgcttttt tgaccttcac ttgctgttcc tcctaaacga cgattaacta
     1741 ttgatcatct atttaacgaa gagaaaattc attctccatt tttccgtcac gtaacatgat
     1801 ttcttttgct gcttttttga tatcttctcc attatacaaa acattataga ttgtttgagt
     1861 aattggcatc tcaacaccta gctgttgagc tagctctact gctgcttttg tcgttgccac
     1921 accctcaacg accattccca tgttttctag aacttcttcc agcttatgtc cctgtcctaa
     1981 caagttaccg gcacgccaat tgcgagaatg aacacttgta caagtaacga tcaagtcccc
     2041 cacaccactc aaaccgataa atgtcagagg attggcaccc atcgcgacac caagacggct
     2101 gatttctgcc aatcctcgag tcatgattgc tgcttttgca tcatctccga atcctaatcc
     2161 atggatagcg ccggcaccaa ttgcaatgat atttttcagt gctgcaccag tttctacgcc
     2221 aatcacatca ttatttgtat aaatcctgaa ataatcattc atgaacagtt tttgtacata
     2281 ggctgctgct ttttgatcaa tacttgcagc tgtaattgtt gtgatatcat gtactgccac
     2341 ttcctcagca tggctaggac cagataatac aacaattgcc tgtctttttt cttctggaat
     2401 ttcttcagcc aaaacttctg agattcgttt atgcgtatct tgttctagtc ctttacttgc
     2461 atgtataatg attggcttag tattcatttt accaataagt tcctgtgcga ctgaacgaat
     2521 cgcttttgtt ggaacaacaa ataaaatagc atctgcgtta tccactgctt ctgccaattc
     2581 tttatatccg ataatcgatt ctgggatcgt caaatcaggt aaaaaatgct gatttgtatg
     2641 atacttattg atttcatcaa tctgttcagg tacatttccc caaatacgga catcatgccc
     2701 gttttctgcc aatgtttgtg ctaaagcagt gccccaagat ccaggaccaa gaactgcgat
     2761 tttttgtttc atggatattc ctccaaataa ataattgata atttccatta tagtatacat
     2821 ttcttaaagt tgggactaat ttccattgtt tttaagtgtt tgtttacttc tt
//
LOCUS       DAZKOW010000172         2868 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000172 DAZKOW010000000
VERSION     DAZKOW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.177"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..981
                     /locus_tag="ACG912_002822"
     CDS             <1..981
                     /locus_tag="ACG912_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFQ0514080.1"
                     /translation="YLTIKDYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMI
                     GGVFWNYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVF
                     IPELFIIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFT
                     IQGLLEYASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVS
                     EFYKSFGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVI
                     AIMSVFFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            978..1697
                     /locus_tag="ACG912_002823"
     CDS             978..1697
                     /locus_tag="ACG912_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 32 protein"
                     /protein_id="HFQ0514081.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1712..2797
                     /locus_tag="ACG912_002824"
     CDS             1712..2797
                     /locus_tag="ACG912_002824"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFQ0514082.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
BASE COUNT         1010 a          313 c          557 g          988 t
ORIGIN      
        1 tatttaacaa ttaaagatta ctataaagta ttcgctataa ttgaaaaatt attctggatt
       61 tcactgccac tttgtttaat tgaaagattc atggtcacct atccaactgg aacaattgta
      121 ggagatatga taggtggagt tttttggaat tattcaggtt gtaatatgcc attgaattta
      181 attttgtgtg tgacagttac tgttgttaca atcggatatt tcaagggaga aaaaaatttc
      241 atccatttta ttttatgtgt ttgtgcagca ctttttatgt ctgcattagc tgaattgaaa
      301 gtttttattc ctgaattatt tataatattt gttgcaatag tattcacttt aaaagttgat
      361 tttaaagtag tgatcaagat attatcatgc gtaatacttg tgtcgatagt attatccttt
      421 tttataacaa tatttattac attgaacgat aatggacaaa gctatgctga taatttcaca
      481 attcaaggtc ttttagaata tgcgtcaaga gattctggat atgatggtgt tggtgattta
      541 aatcgaataa ctggtatctc tagagtagta gatgaaatat ttaataataa ttttagatta
      601 gtcctaatgg gagtaggcat tggcaatgca gagtttacca atttttttgt cagtgagttt
      661 tataaatcgt ttggtaatct tcattatcag tattttcatc atatttggtt atttatagaa
      721 gttggaatat taggagtttc gatattccta ttttttataa taaagacatt tttcaatgca
      781 aaaagatctt ttatgaagga taaagatgca agtcttgtta aagttattgc aattatgtcg
      841 gtattctttt ttgtgtataa cacaacactt aggggtgaaa caacggggcc actgatttat
      901 atgattttag ccatgcctga agttgtgaaa aggaaagagc attattatag tgaaaaaaat
      961 acaaaaggag agatattatg attcctaaga agatacatta ctgttggttc gggggaaatc
     1021 cacttccgaa aaaagataga aagtgcatag aaagctggaa aaagtattgc cctgattatg
     1081 agattattga atggaatgaa tcaaattatg atttaaataa gaaccgttat atggccgatg
     1141 catataaaga acgtaaatgg ggttttgttc cagattatgc aagatttgat attatttata
     1201 atcatggtgg attttattta gatacagatg ttgagctaat taagtcgtta gatatacttc
     1261 gtgaaaatca tgcctatatg ggatttgaag gggaaatctg ggttaatggc ggtattggtt
     1321 ttggtgcaga agaaggacat tctgttatta aaggtttaag ggatatttac gattcattaa
     1381 gtttttatca aaatgatggt acattgaact tgaaaccgag tccgtattat attacagatt
     1441 atcttgaatc tattggattg gttcgcgata acagcgaaca aataataaat ggcctgcata
     1501 tctatcctac ggattatttt gctgccaagg attatgatac tggaaaaatt tataaaacta
     1561 gtaatacaat aagcattcat caatataatg catcttggaa aacacctaaa caaaaatttg
     1621 tttatagatt aaaatcaaca attggacaaa aaaaatttaa caggttactt gcactgaaaa
     1681 aatttttaca cttataaagg agaaaagtac tttgaagaaa atttgtttca ttagtggtgt
     1741 aatttctcgt tctgggggaa ctgaaagagt agggagtatt attgccaata gcttgtctga
     1801 tttaggttat gatgtcacta ttttaagctt ttggaatagt ggagagcctt attttgggtt
     1861 agataagacg attaaggttg attatatgtt aaatcctaaa acggagggaa aattatatag
     1921 aacttattta tatccggttt ggaaattgca caagtatata tgcaaaaatg aatttgatat
     1981 agtaattgat attgatactt tgttgtcttt gtatactaca agggcaatta aaggaacaaa
     2041 gtgcaaattg atttcttggg aacattttaa ttactggaca atggaaatgc tgaaagaaaa
     2101 gaagagattc aaggctaaaa aattgataaa aaaatatgca aagaggcttg tggttctcac
     2161 agaagaagat cgtcaaaaac atattgatga atatagttta tcagaagatt ttgttataac
     2221 tatgccaaat ccgtgtatat ctaatatcga gtacgaatat cattatgata aaaagagatt
     2281 tcttgcagta gggcgattag tggaagagaa aaattttgct gacatgataa gtgcttggaa
     2341 aattgtagaa aaaaaagtag atgattggga tcttatcatt gtaggaaaag gtgaattaga
     2401 aaaagatctt cgccagcaga taaaagattt aaatctgcaa agagtaacta tagcagggca
     2461 ttcagaccat gtggaggaat attataaatc tgcatcttgt tatgtgctta gttctgttta
     2521 tgaaggattt cctatggtta tattagaagc acagtcttat ggattaccag ttatttctta
     2581 tgattgtaaa actggtccga gagatttggt acatcataat tttaatggga tgttggttga
     2641 agataagaat atagaccaat tagctaagag catgataatg tttactaaga atactgattt
     2701 agcaatgaga atgtcactga acgcatataa taatgtacaa aaatttaatt tgaaagaaat
     2761 tacaaaacaa tgggttgctt tgattgaaaa tgtatagttt tttgtggttg tccaaacatg
     2821 aaaattagcg taattaatca aagagctaga agaaacctat tacatttc
//
LOCUS       DAZKOW010000173         2785 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000173 DAZKOW010000000
VERSION     DAZKOW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.179"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            231..1556
                     /locus_tag="ACG912_002825"
     CDS             231..1556
                     /locus_tag="ACG912_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFQ0514083.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            1549..1899
                     /locus_tag="ACG912_002826"
     CDS             1549..1899
                     /locus_tag="ACG912_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514084.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            1896..2066
                     /locus_tag="ACG912_002827"
     CDS             1896..2066
                     /locus_tag="ACG912_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514085.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
BASE COUNT          982 a          441 c          539 g          823 t
ORIGIN      
        1 ttttctaata taactaagca aaactggata atacgacact tattttcaaa ttttcacaag
       61 actaatagta aatatccatt taataaagtg atacaatgtg ttgattttac aaatcaacag
      121 aagaagtagg gaaataactt acctatgctt ctttttttgt acatttaatc gattcttctt
      181 tacaaaaacg aacgtatgtt tgtataatgt ggaagaatgg agtgttattt atgatggatt
      241 attcaaaaga gccagtaaat gattactttt tgatcgatat gaaaagcttt tacgcaagtg
      301 tcgaatgtat agaaaggaat ttagatccat taacgactga attagtcgtc atgagtcgag
      361 cggataatac aggatcaggg ttgattttag cttcttcacc agctgcaaag aagaagtacg
      421 gtatttctaa tgtcagtcga ccacgagatc taccgcaacc ttttcctaaa acgttacatg
      481 tcgttccacc acgcatgaat ctctatatta agcgaaatat gcaggtaaac aatattttca
      541 gaaggtatgt agcagacgaa gatttattga tttactcgat cgatgaatcg atcttaaaag
      601 tgaccagatc aatgaatctt tttacgactg aaggaacaag aagtcaacgg agaaagaagc
      661 tggctcaaat gatccaggag aatattaaag aagagctagg attgcttgct acagtaggtg
      721 tcggagataa tcccttatta gctaaacttg ctttagacaa tgaagcaaaa cataatgatg
      781 gattccttgc tgaatggacg tatcaaaatg ttcctgataa agtatggaat attcctgaaa
      841 tgacagacct ttggggtatt ggatctcgaa tgaaaaaaag actcaaccaa attggtatca
      901 tgagtattaa agacttagct aattggaatc catacactat taagagccat ttaggtgtga
      961 ttggcttaca gctgtatttt catgcgaacg ggatcgatcg aacagatata gcgatcccac
     1021 cagaaccgac aaaggaaaaa tcatatggta attcacaagt gttaccacga gattatacca
     1081 ggagaaatga aatcgaactg gtggtaaaag aaatggcgga acaagtagct attcgtatca
     1141 ggcaacataa ctgtaaaaca ggttgtgtcc atttatacat tggcacgtcg atcttagaaa
     1201 cgagaccagg tttttcacac cagatgaaga tccctataac agataataca aaggaattgc
     1261 agaattactg tcttttcttg tttgataagt attatgaagg acaagaagta aggcacgttg
     1321 gaataaccta tagcaagctt gtgtacactg attcgcttca gctggattta tttagtgatc
     1381 cacaaaagca aattgacgaa gaaaatttag ataaaattat tgacaagatt cggcaaaaat
     1441 atggttttac gtcgattgta catgcttctt ctatgttaga aggcgctcgt agtattacta
     1501 gaagtacatt ggttggtggc catgcaggcg gcgcaggagg gcttaaaaat gattgattat
     1561 aatgatcgtg gacgtttaaa gtgggcaggt ttttatttat ctgatcatac tgaaaaaatt
     1621 gatgccgaca atgaacaacg agccaatcaa aatttggcaa aagaacaaat gacgactgaa
     1681 gaggttgcgg aagtattgaa cgctgcagtc ataaaaaata gatcagtatg tattcaaaaa
     1741 gaagaacgaa acgctgaagg atactatcca gaagatattg ttagcaagat cttaggtagt
     1801 gatgagctgg ggatctacgt tggaaaaaac aaagttcact acgacgaaat acgtcatatc
     1861 gaattttacc aggataataa atggggtcga ttagaatgaa cattatccag caatatgagc
     1921 taaagtatat aacctttgat cagctatcag aagagatttg gggttatggc cagcgtttga
     1981 taaatgaggt tggtgttgaa cgcttctctt tctatgtaga agctgcagca ggataccata
     2041 aatacgaata ctatattggc tattaaagtg caataaaatt atatttcttt tatgttttac
     2101 attttgtatc aattctgata aaattaccta taggtaatta aaaaatataa aaaaactagt
     2161 tacaacctta ggacgttgta actagcttga agcatactag agctaactaa aatagcacta
     2221 gaaagcaatg ttaaacccgt ccaacaaact tgatttaaca ttgagaccag cttattcagc
     2281 taaatatagt tgaataagct tcttttgttt taaaaaaaca aaaggtgtac tacgaataac
     2341 gcaagtaaaa gaaatactat tacaaccagt aggctacaag taagtattac taaacattgc
     2401 gtagttagaa cctttgctct attgtctaaa gccacaaaat tagagatcag ctttatgatg
     2461 ctaagagcga gctgcatcca tgcggacata ggttcttttg acactattaa ttctcctttt
     2521 taggataagg gaaattgcta gtagtttttt cattagcacc atcccccttc atttgattga
     2581 gtctagtgct gctgtgatag tttgctgttt aatatgctta cattcttatt ataacataaa
     2641 tgaaacaaac acatggttag aggtcgtatc aatcaaaata gtgtctcaca aacgatttat
     2701 tacttactcc tgctgactaa cggactgttc ttcagtaact gtaactatat gtttgttaaa
     2761 accaccatta cctgtagata aaaac
//
LOCUS       DAZKOW010000174         2707 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000174 DAZKOW010000000
VERSION     DAZKOW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.180"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            171..515
                     /locus_tag="ACG912_002828"
     CDS             171..515
                     /locus_tag="ACG912_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFQ0514086.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            516..935
                     /locus_tag="ACG912_002829"
                     /pseudo
     CDS             516..935
                     /locus_tag="ACG912_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1027..1377)
                     /locus_tag="ACG912_002830"
     CDS             complement(1027..1377)
                     /locus_tag="ACG912_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514087.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1343..2674)
                     /locus_tag="ACG912_002831"
     CDS             complement(1343..2674)
                     /locus_tag="ACG912_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFQ0514088.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1002 a          490 c          522 g          693 t
ORIGIN      
        1 taaaaaaagc gttagcaact atgcttaaaa agcatatcga ctgaataaac taagcattgg
       61 acaggctgaa agactacatt taaaatgaaa tgcgaagaaa caatagaaaa taaagttgcc
      121 ttagaaccat agttgaatca cctaacagtc gtgaagggag aataagagaa atggcagaaa
      181 aacaaaccgc aggacgagat caattaggag attttgcacc aaaatttgca gaattgaatg
      241 atgatgtttt atttggacaa gtatggtcca gagaagaatt atctgctcat cagcgtagta
      301 tcatcaccat atctgcatta atttcaggag gaaattttga acaattgcca gctcatctga
      361 agattggcaa gcaaaatggc attactagtg aagaaatagc agaaatcatt acccatttag
      421 ctttttatgt aggatggcca aaagcttggt cagcttttag tttagcaaaa gaaatctatt
      481 tagaagaaaa tagcgaagat aagaaagagg cgtaaatgac gacaaaacac gaagaaatac
      541 acaatggcgt gatttttcca gcaggtgaaa aaaacgaagc ttacgctcaa gtatttaccg
      601 ggtaaagcta tctgaatatg ttagtgaatg atcccgaagt ttctgttggt gtaggtaatg
      661 taacttttga accaggttgt cgcaatcact ggcatgtcca taaagacggc tatcaaatct
      721 tacttgtgac cggaggagaa ggctggtatc aggaagaagg aaagccagca agacgattaa
      781 tggctggaga tgtcgtcgta acgcacaaag ggattaaaca ttggcatggt gcgacaaaag
      841 acagttggtt tgaacatttg gcgatcactg ctggtaattc agagttttta gaacctgtaa
      901 cagatgaagt gtatgaagga ttggaaaatc aataatgtca agcttgaaca gctcctgaaa
      961 gtagatttta atcgaacttt caggagctgc ttttttgatt cagtctttta taaatagaaa
     1021 atagggttaa taataggaat gagaagcacg tcgaaagatc ttaatattga taatcggctg
     1081 ggcatacatg atttccacgt catcaatcaa actacctaac agatgataat cttcccgatc
     1141 atgtcgggga ttacctaata gttcttgata atagatttcc atactagggt cccgaggatt
     1201 gttcagcaaa tcagcaagta catctaaatc ttctggctga gtttctgccg taccctgtat
     1261 ctctatgaaa agcccttcct cagcaaattc tactccaata cgtatttcag ttatttcatg
     1321 ggaatataaa taagtaagaa gctcatcgat gattcttttt gataattctt tttccaagtg
     1381 tctttttttc ctttctgaca gcggtaaagt tatctagtat cggtacaagg atacctgcaa
     1441 cgaaaccagt tgaaaaacca ctgttatata gatttagtcc tccgtgcaaa gtgctgacgt
     1501 tatggacaag agcaatatgt agtacaccag ctattacacc gtaaaaactg ccgtaaaaac
     1561 cgcttatggg cgcgagacct gtcccaaaca cagctgcaac aagcatgcca gtactggtga
     1621 tctcatgaaa agttccaaac aaagaagcaa taaatattcc gactagtatc ggaaaactgt
     1681 tttttaaatg acaaccaaaa gcactgaaac ccaccgcaga taaaatagca ccgatgacag
     1741 gtccatttaa ctgaccgcca accagcaaaa cataagaaga cagcatcaga ccaactaagc
     1801 ctatgttgat caaagtagca ccaataccag aattagcaat gaaatcagtt gtcaattttc
     1861 cagatgaatc gaaaatttct cgaatcccca aaagccgaaa ggaattataa tagaaaccaa
     1921 ttccgactgt cagcgaaaaa aagccaaaaa taatccaaat gagaggaaag tgatattcat
     1981 tgaaaaccaa tgtattttcg acgattgaat agctgaataa cctcaaaaca gcagtaaaag
     2041 tcatcgcaat caacccagag gtgaatccaa tattataaag tgtgaatcca cgatggaaaa
     2101 ctaaagtttg agcagctaag ggcggaagaa gtagaccaat tgctatgcca gctaaattcc
     2161 ccaataaaat acctatcagt aaaggaaaac gcataccaaa agtgatataa ctaacgaccg
     2221 gtgataatgc gctaccaaac agtccgatca tgatgtactg agaaaaaggt ttattgacaa
     2281 atacagcgta tagatacaca cctaaaatga ttgagataga attataaagg tttttgccaa
     2341 aaaaagagaa accggtaact gttagaatcg aagcaaataa gggaccgttt aaaatcacct
     2401 tgtgccgata agctaaaagc acagaaagta aggtcaaaat ccctacattt atgaatgcgc
     2461 ttccgaaacc gcctaaagca aaataatctg tcaaaagatt gcttggcgag gtaagaatag
     2521 ttagcaatcc actccaatta tcagtaggag tattagaaag caagccaata aaaagaaaaa
     2581 aaacacctgt aaaaccaaga taaaaatact ttcttttctg tgaaacgatt cgtttttgag
     2641 gatgaatata aataacgtca gtttcgtttt ccattacaca ccttcttttt cattttcttt
     2701 aactata
//
LOCUS       DAZKOW010000175         2652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000175 DAZKOW010000000
VERSION     DAZKOW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.181"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>2652)
                     /locus_tag="ACG912_002832"
     rRNA            complement(<1..>2652)
                     /locus_tag="ACG912_002832"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          551 a          802 c          557 g          742 t
ORIGIN      
        1 gggaaatctc atcttgaggt gggcttcaca cttagatgct ttcagcgttt atcccttccc
       61 tacatagcta cccagcaatg cccttggcag aacaactggt acaccagcgg taagtccatc
      121 ccggtcctct cgtactaagg acagctcctc tcaaatttcc aacgcccgcg acggataggg
      181 accgaactgt ctcacgacgt tctgaaccca gctcgcgtgc cgctttaatg ggcgaacagc
      241 ccaacccttg ggaccgacta cagccccagg atgcgacgag ccgacatcga ggtgccaaac
      301 ctccccgtcg atgtggactc ttgggggaga taagcctgtt atccccaggg tagcttttat
      361 ccgttgagcg atggcccttc catgcggaac caccggatca ctaagcccga ctttcgtccc
      421 tgctcgactt gttggtctcg cagtcaagct cccttctgcc tttacactct tcgaatgatt
      481 tccaaccatt ctgagggaac ctttgggcgc ctccgttacc ttttaggagg cgaccgcccc
      541 agtcaaactg cccatctgac actgtctccc accacgatta gtggtgcggg ttagagtggc
      601 cataacgcag gggtagtatc ccaccagcgc ctccatcgaa actagcgttc cgatttctac
      661 ggctcctacc tatcctgtac atgcggtaca gacactcaat atcaaactac agtaaagctc
      721 catggggtct ttccgtcctg tcgcgggtaa cctgcatctt cacaggtact aaaatttcac
      781 cgagtctctc gttgagacag tgcccaaatc gttacgcctt tcgtgcgggt cggaacttac
      841 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt tactggggct
      901 tcaattcgta ccttcgcttg cgctaagcac tcctcttaac cttccagcac cgggcaggcg
      961 tcagccccta tacttcatct tacgattttg cagagacctg tgtttttgat aaacagtcgc
     1021 ttgggcctat tcactgcggc tgatcgtatg atcagcaccc cttctcccga agttacgggg
     1081 tcattttgcc gagttcctta acgagagttc tctcgctcac cttaggattc tctcctcgac
     1141 tacctgtgtc ggtttgcggt acgggccgct gttttctcac tagaagcttt tctcggcagt
     1201 gtgacatcag gaacttcggt actattattt ccctccccat cacagcttgt ccttaagaaa
     1261 agaagcattt gactcctctt cagacttact gcttggacag acatttccga tcgtctgcat
     1321 tccttagcct cctgcgtccc tccattgctc aaacaaaaac aacgggtaca ggaatatcaa
     1381 cctgttatcc atcgcctacg cctgtcggcc tcggcttagg tcccgactaa ccctgggcgg
     1441 acgagccttc cccaggaaac cttagtcatt cggtggacag gattctcacc tgtctttcgc
     1501 tactcatacc ggcattctca cttctaagcg ctccagcagt cctcacgatc tgccttcaac
     1561 gcccttagaa cgctctccta ccaatacacc taaaggtgta ctccacagct tcggtaatat
     1621 gtttagcccc ggtacatttt cggcgcaggg tcactcgact agtgagctat tacgcactct
     1681 ttaaatggtg gctgcttcta agccaacatc ctagttgtct gtgcaacccc acatcctttt
     1741 ccacttaaca tatattttgg gaccttagct ggtggtctgg gctgtttccc tttcgactat
     1801 ggatcttatc actcacagtc tgactcccgg atatgaatga atggcattcg gagtttatct
     1861 gaattcggta acccgagatg ggcccctagt ccaaacagtg ctctacctcc atcattctca
     1921 attccgaggc tagccctaaa gctatttcgg agagaaccag ctatctccaa gttcgtttgg
     1981 aatttctccg ctacccacac ctcatccccg cacttttcaa cgtacgtggg ttcggtcctc
     2041 cagtgcgttt taccgcacct tcaacctgga catgggtaga tcacatggtt tcgggtctac
     2101 gactacatac tcaaacgccc tattcagact cgctttcgct gcggctccgt ctcttcaact
     2161 taacctcgca tgcaatcgta actcgccggt tcattctaca aaaggcacgc catcacccat
     2221 taacgggctt tgacttgttg taggcacacg gtttcaggtt ctatttcact ccccttccgg
     2281 ggtgcttttc acctttccct cacggtactg gttcactatc ggtcactagg gagtatttag
     2341 ccttgggaga tggtcctccc ggattccgac ggaatttctc gtgttccgcc gtactcagga
     2401 tcctcctagg tgtcttccac atttcgtcta cggggttttt accctctttg actgactttt
     2461 ccaagtcatt cgactatctg aaagaactac catattggag tcctacaacc ccaacaagca
     2521 agcttgctgg tttgggcttt tcccgtttcg ctcgccgcta ctcagggaat cgaattttct
     2581 ttctcttcct gcaggtactt agatgtttca gttctctgcg tctacctcga atgtgctatg
     2641 tattcacaca at
//
LOCUS       DAZKOW010000176         2471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000176 DAZKOW010000000
VERSION     DAZKOW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.183"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            192..710
                     /locus_tag="ACG912_002833"
     CDS             192..710
                     /locus_tag="ACG912_002833"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFQ0514089.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            838..1521
                     /locus_tag="ACG912_002834"
     CDS             838..1521
                     /locus_tag="ACG912_002834"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFQ0514090.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1830..1903
                     /locus_tag="ACG912_002835"
     tRNA            1830..1903
                     /locus_tag="ACG912_002835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1864..1866,aa:Arg,seq:tct)
     gene            2062..2421
                     /locus_tag="ACG912_002836"
     CDS             2062..2421
                     /locus_tag="ACG912_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFQ0514091.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          852 a          363 c          487 g          769 t
ORIGIN      
        1 acttgtaatg ttagggtaaa cgttttttaa actaacgtca attatttata ataaaaaatg
       61 agataaagat gaggaaatag aattagaaac atataattat taaagggaaa catttttaca
      121 gctataattt gaaaaaattt atcgttatta tatgattaag aaggattgaa taaagaaaga
      181 agttgaaaac gatacctgat tacaaaaaaa taacttttat aagttctata acgttgatta
      241 tatttttaat tcttattctt ctcgtcgtct tggttccccg aatttttggt ttgactcctt
      301 atattattga ggacaaagaa atgagtccat attatgcaca aggaaatctg atatatgtac
      361 gaccgaaaaa tacaaatgag attttagtcg gagatattat tacatattat gaaaattcag
      421 gaaaaaaaat ctcgacgcgt cgcgtaatag ccgttgaaaa caaccacgaa gatttttata
      481 caaaagctga tacaaagact tatattgagc ctggagtagt aaaaaagaga aatattatcg
      541 gtagtcccat ctttcagatt ccatatattg gactagtcct ttcaaaaacg gcatttgcat
      601 ggattcaggg gttattcttt acgattgctt tttgcctaac aggaatcaca gcatggaata
      661 cctttgtaga gttgaaaaag aagcggagac gaacacagaa atttttgtaa gaatgttaaa
      721 aacataaatg tattccagag atccaacgac tcttctgaac atgaaacacg agaaaacact
      781 catgcagctt gtagatttag tcagttgttc gtataatgtg tgaagaggag gagtagtatg
      841 cgcaatgaaa tgatgcatcg tcctgtagtc aaagaagaac tattggattt tatgcgtacc
      901 cagcaaaaaa aattaccggg tgagctaggc aaactggaag aagaagctca tgtagcagaa
      961 gtaccagtta ttccccatga aacagttgta tttttgaaat ttttacttgg acagcttcag
     1021 cctgaacgaa tcttggagat cggagcagct atcggttttt catcaagtgt catggccacg
     1081 acaatcggag aaaacgctca tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa
     1141 gctaaaaaaa actatgaacg tctaggactg acagataaag tgactttgct tgaaggtcaa
     1201 gcagcagata tcttgccgga gttgtctggt ccgtatgact ttatttttat ggatagtgca
     1261 aaatcaaaat atattgaatt tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt
     1321 ttgatggtag atgacatctt ccaaggagga acgatcttgc tcccagatga agagattccc
     1381 cgtggcaaac gagctatcca tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat
     1441 cccgatttga catcgacaat cttgccatta ggtgatggtg tgatcttaat gacaaaggaa
     1501 tcagaaacaa tcgagttgtg atacatgatt atggcagcct gtagataatc gattgaatcg
     1561 agggctcaat tcgaacttga tgtaaaagtt tctatatttg taagtcaatt aaaggataca
     1621 ggaaatctaa ataaacagga gtagcatgaa gtcaatgtag ctcctgtttt caattgataa
     1681 ccttagaaca gctgaaggag aatgtctcgt tttaaaagat tgatttagta gttgggccaa
     1741 aataattcat tatttgctaa ttaaataact atttcgtatt gacagaagca atttaattat
     1801 ctataattat tgatgttccg ctgattcttg tcatagtagc tcagctggat agagcatccg
     1861 ccttctaagc ggacggtcgg gggttcgaat ccctcctgtg acgctaaaaa accgttagaa
     1921 actgaggttt ctaacggttt ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc
     1981 aaataaaata aaaaaacatc cttatcgagt acaattataa ggagcaaaaa aataaaactc
     2041 atcagaaaag aggaagaaga tatgacgata ctatctagaa ttttagtgac tctagttgca
     2101 attgaatttt tttatatcat gtatatcgaa acggtgagaa ctgattctga cacgacaagt
     2161 agggttttta aaatgagtaa agaagagtta agcagaaaat ctgttcaaac attgtttaag
     2221 aaccaaggag tgtacaatgg actattagga gtgggactac tttatggagc ttatctttct
     2281 tccgcatcta aagaaatcac aagtatgcta ttaatatcta ttttttttgt tgcattatat
     2341 ggcagtttga ctagcgatcg ttggatttta ctcaaacaag gtgggctagc tctgttggca
     2401 ttgctttcta ttttcttata aatatagaaa aaataagaat ttatctaaca ggaaactttg
     2461 tgaaaaatat t
//
LOCUS       DAZKOW010000177         2433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000177 DAZKOW010000000
VERSION     DAZKOW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.184"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            133..2286
                     /locus_tag="ACG912_002837"
     CDS             133..2286
                     /locus_tag="ACG912_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFQ0514092.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          831 a          427 c          539 g          636 t
ORIGIN      
        1 tgctgtgaaa caaaaagttg attttatctt gaaattaggt aaagaatttc ttgctccgcc
       61 cattttttct tttttattaa gaagtttttg ggtacaatga agcatgaatg taaataggcg
      121 gaaggatagg aaatgatgaa agattttatg aaaaaaatat taaatacttc ttggttagaa
      181 aaaataagaa aaggtgcaca agaacctaag aacaaaacgt ttcgccggtc ccatgttcct
      241 tttcgcttaa attttctttt tttcattata tttacgttat ttgttgcgtt gattgctaga
      301 ttgggttatt tgcagatcgt gaacggcgaa gagatggagg cgcgagtgaa gtctacttct
      361 acgattacgg tccaagaaag tactcctcgc ggaatgatct acgactccac aggaaaagct
      421 ttagttacga ataaagcgaa tcaggccatc acatatacga gaggaacaca agttacggct
      481 aaagatttgc ttgaaatcgc aacgaagctg aactcgctga tcgatgttcc ggtagatgaa
      541 aatctgacag atagagataa aaaagatttt tggctggctg atcccaaaca tttgaaagaa
      601 gccacaaaaa gactttcggc aaaagaaaag caattaggga acagcgaaca atatagtgcg
      661 atggtcaata aagtcacagc tgatgagatt aatttaaacg aggagcaatt aaaaattgct
      721 accatcttta aacggatgaa tagtgcatat gaattaaaca ctgtctatat caaaaactct
      781 ggtgttactg atcaagagtt agctgttgtg gcagaacgcg cttctgaact tccaggagtt
      841 tcgactggca cagactggac acgagaatat aatgcagcaa gttcactgaa aagtattctt
      901 ggcagtgtga cgactgaaaa gcaaggactt ccagcagatg aagcagagaa atacctagca
      961 aaaggttatt ctcggaatga ccgtgtagga caaagttatt tggaaaaaca atatgaagat
     1021 gtcctgcaag gaaccaaaac ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg
     1081 aatcaaaaag aaatcttctc tggggaaaaa ggttctaatt taatgttatc tatgaatgca
     1141 gaattccaat caaaagtaga agaaattttg aaaaggaatt atcaaacatt gatcaacaac
     1201 ggcaaagcac agtattcgcc tggtgcttat gcagtggcta tgaatcctca gacaggagaa
     1261 gtgctagcaa tgactggttt cagtcatgaa caaggttcca aagaaatcac cgagaatgct
     1321 ttaggcacga ttactagcgc ctttgatcca gggtctgtag tcaaggctgg tactttgaca
     1381 gcaggctggg caagtaacgc gatcagcgga aatcaagttt tgattgatga gccgattcgt
     1441 ctgcaaggtg cttcagaaaa aagctctgtc tttaatcgaa gcggacaagt tgctcttgat
     1501 gcagtcaaag catcggaatt gtcttctaat acgtatatga tcaaagtggc gttaaagatg
     1561 ttaggactag actatacacc tggaatggga ctacctagtt tagatgaaga agctaaagct
     1621 tatcagcaat tgagagactc tttcaaagag tttggtcttg gtacaacgac tgggatcgat
     1681 ttgccgaatg aatctcctgg gatctcacgt tctgttgatt atatgaagaa gttcaatgcg
     1741 gataatggca aagaatggta tgctccaggt aactttaccg atttggcatt tggacagttt
     1801 gacacatata caccaatcca attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa
     1861 cgagtacagc cgcacctagt caaagcgatc tatggcaatg acgaaaatgg aaacttgggt
     1921 gaagtgaaga aagaaattgg cacaacagta gaaaatacgg tcaatatttc tgcagaaaat
     1981 atggcgatcc tccgtgaagg attccaccaa gttgtccatg gtaccgatcc ttacacaaca
     2041 gccaaaccat tagccagtgc gaaaatggac ctttccgcaa aaacagggac tgcagaaacg
     2101 gttgcggaag gtcatccaga tattgcgaca gtcaatagta atatcgtggc atacggacca
     2161 acagacaatc cggaaattgc catcagtgtt gtcttgccga acttattaga cgaacaagac
     2221 cacataaact tgacgattgc taaagaaata atggacgcgt attatgatat gttcatggca
     2281 aaataaaaaa gaggtcgtga aaaagccgct tagtttcgag tatgaagaaa aattttgtga
     2341 aaatagcttc tcacattttt gcttaaattt gacttcatac cgagaaactg gcttttgaac
     2401 accgtttact cggcaaaaaa ggttgtgaca aaa
//
LOCUS       DAZKOW010000178         2419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000178 DAZKOW010000000
VERSION     DAZKOW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.185"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..1150
                     /locus_tag="ACG912_002838"
                     /pseudo
     CDS             6..1150
                     /locus_tag="ACG912_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            1321..1779
                     /locus_tag="ACG912_002839"
     CDS             1321..1779
                     /locus_tag="ACG912_002839"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFQ0514093.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="ACG912_002840"
     CDS             1938..2408
                     /locus_tag="ACG912_002840"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFQ0514094.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          810 a          454 c          456 g          699 t
ORIGIN      
        1 gaaacatgca cattcaaaca aaattgatcc acggaggcat cagtgaagat cccacaacag
       61 gagctgtcag tgtacctatt tatcaaacat ccacttatcg gcaagatggc gtcggccagc
      121 ctaaacaata tgaatattca agatctggta atcctactcg ttttgcattg gaagaactga
      181 ttgctgattt ggaaggcggt gttcgaggat tcgctttttc ttctggtctt tctgggattc
      241 atgccgtttt ttctcttttt caagctggcg atcatatctt attaggagat gatgtctatg
      301 gaggaacatt ccgtctattt gacaaagttc ttactaaaaa tggtctcgag tacacgatca
      361 tagatacgag taatcttgat aaaatagaac aatccatcaa acccaataca aaagcacttt
      421 atcttgaaac acctagcaat ccgcttctaa agattactga tttagaaaaa agtgccactc
      481 tggctcatca acatggattg atagttattg ctgacaatac atttgctact ccttattttc
      541 aaagaccttt agatctcggt tctgatatcg ttgtccatag cggtaccaaa tatctaggcg
      601 gacacagcga tgtagtagct ggtttagtga cttctaacca aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaa
//
LOCUS       DAZKOW010000179         2397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000179 DAZKOW010000000
VERSION     DAZKOW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.186"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            206..2068
                     /locus_tag="ACG912_002841"
     CDS             206..2068
                     /locus_tag="ACG912_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFQ0514095.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2068..>2397
                     /locus_tag="ACG912_002842"
     CDS             2068..>2397
                     /locus_tag="ACG912_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFQ0514096.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          864 a          353 c          466 g          714 t
ORIGIN      
        1 aaaatttgaa aaagcgtttg attatttata ttacgactta gtgcaataaa aaacgctttt
       61 taacagtaca tgtagtcgtc tttataatag ataaactctg taatattagt taaaaagcta
      121 taagttgcac ttagttaagt gcaaacattt catattgtcg ttagtttaga aaacgcttat
      181 attagcaatg taaggagaga tgataatgca gcagctaaca aaaagagaaa ggcagctttt
      241 gatttacttg gtggaaatgt ctgaaccggt aacaatcaaa aagttggctg aaaaagaaaa
      301 tgttagtgtt cggacgataa aatacgatct agatgatata agagagtggc tgaatgagcg
      361 gaagcaagtc ctatatagca aacgtagtca gggaatttgg atgaatatta gtagctctga
      421 tcgaatcaaa ttaaaaagcg aattgatgga tgttaatcgt tttgaaatat ttgcagatca
      481 aagtctccga atcaaacgtt tactaattat gcttttagta acaaaagaag ctattactgg
      541 aatgaaaatg gccaacgcat tagatgtcag caaagatacg atcatgaatg atttagatgt
      601 gctggaaaat aattttctta aggaaggatt gaccttgaat cgtcaggcgc gaaagggttt
      661 ttggatcata ggagaagaac gtctagttcg attaacgatt gaagagattc ttcaaaaaga
      721 gttcacggat tatgacattt ataaattaat gtcattgtta ttgaatggcg gtgagactga
      781 atactttgaa atgtattctg ctactgctac acctatccaa gaggtattta atcaagtaat
      841 cattcgaatg cgacacttac tggaatttga aaatctagag aaactgaatt acgcggaatt
      901 actaaatatt ctgattcgag tgacaattgc taccgttcgc ttacgcaaag aggctacgat
      961 tggtcgttat caattggttg ctgaacaaga attagacaaa aacgatcttt cttatcgcgt
     1021 catgcagcag gtctttgatt attatcagtt gccactgttc gaggatgaat atcattatat
     1081 ctatagtgat acattcgaca cgattcacca gcaagacgtc atgaaactag ttgagaatgt
     1141 gatttataaa gtaagtaaag caatcaacca ttctttttat caagatccac aattgttgac
     1201 gaatctgtat gctcatttat ccatgcgatt atcgagaaag aaaaaatttg tcaatgaata
     1261 taatcctttt aaagaagata tcaagcagaa atacccggaa ttatttgatg cgatcaagca
     1321 agcagttcga caagaaataa gtgaacaaaa tattctgatc aatgattctt tcgtggcata
     1381 tattgcctta cactttttgg tttcctacga aagagaagaa gatctacgaa gcgttagagc
     1441 cgtctacatt tgttcaacag ggttgggtgt gactagtctg atcaagcaaa agatcaatga
     1501 agaattaaat aatatcacta tcgtggcatt tgcttcagta ttgaatgcgc aagaaatgat
     1561 tgctaccaaa gatcctgatt tggtgattag tatttttcca attaatggca tcgatcgtcc
     1621 tttcatcaaa gtccatccat tacccactga acaggacttg aaaaaaattc aacaaataat
     1681 aaaaaaaata cttgcttctt ccaatcagca ggaaaggcat aacttgaatc ttagagaaac
     1741 gtccatcaat aaagatactt tagaagactt tagcaaggaa ttgattttga aagcttatac
     1801 gatttatgaa cgtttacttg tcattttgga agcagaattg atccaagaat atcgagaagc
     1861 ttttttgctt catgtattct taatggttca tcgtatcaca tttgatcaac aatatatggc
     1921 agaaggtcgt caagtagacc agattatttt agacaaagga acactggtag gaaaaatcga
     1981 agcagttttt gcagaaaata atttaggaat caatcagtca gaaatcagtg cattattctc
     2041 atatataaaa gaaggagcgt caagttagtg aaaatgtcag acgaggcttt aaaaattatc
     2101 gaggaaagtc aaaatcggga aaagttaaaa caagtgattg atctaattga agagcgatta
     2161 gtagaacatg ctattgtacc aaccgagttg cagtggacca ttctaattaa tcacttgaat
     2221 gagatgctga aacgttctca aagaaaggaa acgattcccg tagtcgatcg ggatttgttc
     2281 aaagaagtat ccaaagaagc cataactatt tctgatgaaa tagttagaag tatcggtaat
     2341 ttaaccgatg atgaaatata tgttttgtcg attcattttg aaacagcaaa aaataat
//
LOCUS       DAZKOW010000180         2395 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000180 DAZKOW010000000
VERSION     DAZKOW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.187"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACG912_002843"
     CDS             316..570
                     /locus_tag="ACG912_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0514097.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACG912_002844"
     CDS             571..888
                     /locus_tag="ACG912_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0514098.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACG912_002845"
     CDS             1447..2382
                     /locus_tag="ACG912_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514099.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAZKOW010000181         2372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000181 DAZKOW010000000
VERSION     DAZKOW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.188"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            257..>2372
                     /gene="acm"
                     /locus_tag="ACG912_002846"
     CDS             257..>2372
                     /gene="acm"
                     /locus_tag="ACG912_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFQ0514100.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          946 a          352 c          458 g          616 t
ORIGIN      
        1 aaaattggaa atatagattg aaaaaatgaa agcaataagt aaaataaaat gaatatatgc
       61 gaaaaaataa tttacattag gaaaaacctt gtaaaaacag ttttttggca tatttatttt
      121 ttataaatat ttgtaaaata actatcaatt ttttagttaa aagattggaa atgagtgggg
      181 gaggaattga agaaaggatt tttgagagat gatatagtag gttttatttt tagttaaaat
      241 aggggagata gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt tatttattat
      301 tactaatata acatcactga ttcctgttca tgtctatgcg gatgcaggca gagatatcag
      361 cagtaatgtc acttcgttga ccgtggaccc aacgaatatt acagatggag gaaacataaa
      421 agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt atctttggat
      481 caattggcca agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa tcccattaat
      541 gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt
      601 taatgaaaat attaaaaacc tagactcagt agaaggttgg gggcaatttg agatacaggc
      661 cagaaacgta accgataccg gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga
      721 taaaacagct actgtaaatg tgacaaagcc agcttctggt tcttcttcta gcgtttttta
      781 ttataaaaca ggagacatgc tacctgaaga tactaagcac attcgatggt ttttgaatat
      841 taacaataac ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg
      901 acaaagatta gatccaagta cgtttgaaat taatcaaata cacttaggtg aacagaaggt
      961 ttatagagga gaagagggaa tccaacaatt tttacaagat ttccccagcg ctacatttaa
     1021 tttttcagta actgataatt acattgaaat aactatacca aaaaactttg tcaatcttag
     1081 gaaaataatg gtgagttata agacgatcat agaaaaccca gaacaaataa attttgagaa
     1141 tcattcggaa gcttggttta aagaatttaa taaacctgct gttgatggag aaagtttcaa
     1201 tcatacagtt aaaaatattt cagcttctgg tggagtcaac ggtacagtta gaggcgaatt
     1261 gaagattttc aaatatatca atgacacaga gattggtatc ccaaatgtga ctttcgaatt
     1321 aagacgagca gatgaacagc ctattcaagg acagtctagt atcctgttga cttcgaatga
     1381 acagggagaa gctagtatca aaggcctgca agtaggagac tatgttgtaa aagaaaaaga
     1441 agcacccaat tggattgatt ttgatccact aagttcaaat gaactcaaat tctccataaa
     1501 cgaaaatgat acagagggag tcagtttacc tatttataac aagaaaaagg ttacgaacat
     1561 cacagcaact aaaatttgga acggtggaac cactccaaga ccgagtattt attttaaatt
     1621 attcagagca agtacaaaca attgggaacc agttcctgat gcagaaacaa aacgattaga
     1681 caatggaata acttcagtga cttgggaaga tatccaacag tatgatgata gtggaaacga
     1741 atatacgttt aaagtgcaag aagtagatca aaacggaaat gattatgtcc cttcaggtta
     1801 tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat aaagaagtca tttcagtctc
     1861 aggacaaaaa acatgggaag acaatgagaa ccaagacggc aaacgaccga cgacgatcac
     1921 agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga
     1981 ttggcgctat cgtttcacaa atctgccaaa atacagagac gggcaagaaa tcatctatac
     2041 tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt gacgggtata acatcaaaaa
     2101 cagttataca ccggaaaaaa caaatatatc tataccaggt agccatataa caccgtggaa
     2161 aaattttcca gagaagacag agagagagaa aattagaaat ctattctctc aaaaattgca
     2221 aatatttgct catacaggag aatccagcga acaaagaagc aattatatta gtaagagagc
     2281 tgtacctaaa cagataacaa ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa
     2341 atctagtttt gctgtaatca ttggtttatt ga
//
LOCUS       DAZKOW010000182         2320 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000182 DAZKOW010000000
VERSION     DAZKOW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.189"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(914..1339)
                     /locus_tag="ACG912_002847"
     CDS             complement(914..1339)
                     /locus_tag="ACG912_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HFQ0514101.1"
                     /translation="MHRKVRITRKSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISI
                     EGILRIQGPAFIAQGAHIDIRKNAVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDT
                     LFMDTDSHTIFELNTHRILNKNNKIVIGKHCWIGAKSVI"
     gene            complement(1441..2058)
                     /locus_tag="ACG912_002848"
     CDS             complement(1441..2058)
                     /locus_tag="ACG912_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFQ0514102.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          757 a          442 c          250 g          871 t
ORIGIN      
        1 atatttttga gctatgtaca agaagcattt tattctagct taatttgaca aatcatataa
       61 tttaaaatta acattgattc tcctatgaaa atatctttta cctttctcct gtgaaaagga
      121 aatttccttt tttatagtgt tattgatata atcagaatat tcatcaaact ctgtatctgg
      181 agttaaaaat ctacagtaac atctataagc tttgctagta atcttcttgg gttctctctc
      241 cattgtcaag caacatcaaa acacctactt actattcgat tcttttctgc caaaaacgag
      301 tcagcatcac tcacaactta tgtgttgctg actccgacta gtcctttgtt caatatcaac
      361 ttattaaaca gatataatat ttttctttat ttcgtatact ccaagacttc catctttctt
      421 tcatcttatc tctgaaaaaa acacagcaaa gtgcccttaa taaatataca ttagaatttg
      481 caaatttttt catcttaaga tatatcctat tcatattctt tacaatccta atgggtgatt
      541 acaaatctat ttagttcaag ttctacatta taaacacctt tcttgatagt atcatctgct
      601 gcacaagcga tgtcgatcca cttattaggg gatttacgtg ctcaaaagtg gctattcctt
      661 ttgggaagtc catccataat aatggtccat ggattttttt agggtcttgt cgttcaaatt
      721 tctcaaaaac tttattatac ttccatagtt ttataattat ttggtttcac tttggctcgt
      781 tcaaacacca acttgtattt cttttcaacg ttcgaactgg agccccccct ataatctcat
      841 tattattttc atgacttttt actaccgtgg aattacaagc aacaacaata ttatctgcta
      901 aatgaacacc tttctatatc acgctttttg ctccaatcca acaatgttta cctataacta
      961 ttttattatt tttattcaaa atcctatgag tattcaattc aaaaatcgta tgtgaatcgg
     1021 tatccataaa taatgtatcc cacgatactg tacagccatc tttgaattga atttccttaa
     1081 agcaaattat actcattttt cctgtcgaat taaactttga cccaatatct aaaactgcat
     1141 ttttccttat gtcaatatga gccccttgtg cgataaacgc tggcccttgt attcttagta
     1201 ttccttcaat acttataatc gtttcttcat ttcccgcagt atttaatact ggaaagccca
     1261 aataaactat ttttccagat tgtaatacta tctcaatttt ccctctcgat ttgcgtgtaa
     1321 tccgtacttt acgatgcacc aaaataggta atctccacgc atctgaaata tgaagaaatt
     1381 tataattgaa gtatatcgtt ttaggtaaat ttaatatatt ctttattatc aatccgagtc
     1441 tcatctttca ccatctcttt ttgtaatcaa aattagttta taaaggatta cacccaatat
     1501 accgcccaac gtattatata caaaatccga aatttgaaac gttcctagcc tcaaaaaaag
     1561 ttgtaaaaat tcaattattg ctgaaaaata aaatgcaatt tttcctccat atactattga
     1621 ggaccatatt ttgggtttct ttatattttc atatttaata gacattacca tataaacata
     1681 tggaataaaa agaataatat tttctaaaca cgctgtagat aactcttttg ttccagttgt
     1741 tgtattatac ttataaatcc accagtctcc cataacatca gataaaggat ttaaccaaat
     1801 tgttcgattg attaatgttg taaataaaat catcgcagta tagaatatca aagcacaaac
     1861 ttttctatat tttctatcct tctttaaatg aagataaagt tccattagta tacgtttata
     1921 gccctcacct gcagcaatgc catctgttgc atacataaaa tagaccagtc ctaaaattgt
     1981 caataaaaca gcaaacccaa agggttcata taatttatat aatatatctg taataatttc
     2041 tgcaatcact attatcataa ttatttctcc aatatcaagt atacacttat cgcttccaat
     2101 tacacatgat catattagag tgtattatca cattctctaa tattcaattt atttttccta
     2161 aaatattcga aataatcgtc ttgataacga gtaagtattt ttatacgatt gataacatag
     2221 taacaatatt tttaattata gtaaaaaatc atctgccttt ttcatccacc taatcttatt
     2281 tttcatttta ttcaacatcc ttaaataaac ttccgttcct
//
LOCUS       DAZKOW010000183         2280 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000183 DAZKOW010000000
VERSION     DAZKOW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.191"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1561
                     /locus_tag="ACG912_002849"
     CDS             <1..1561
                     /locus_tag="ACG912_002849"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFQ0514103.1"
                     /translation="KIESLEPYDMIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMI
                     YQMGMEYAGLNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKY
                     FSIIGWIVFTLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLI
                     YAHQRNDVINVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQK
                     MYPDYTPVGELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQN
                     YYFIISAIMSIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFI
                     TLFQPFMIIWVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLIT
                     AMINIVLNIILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLI
                     SLCVIAFVASYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYI
                     LKVLTSVLVKR"
     gene            1739..1960
                     /locus_tag="ACG912_002850"
     CDS             1739..1960
                     /locus_tag="ACG912_002850"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0514104.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT          772 a          239 c          433 g          836 t
ORIGIN      
        1 aaaaatagaa agtttggagc catatgatat gataattaat cgtaaacaaa atgctaaaag
       61 gaatataatt actggaacaa tttttaagtt atatcagatt atcattccct tttttatgcg
      121 tacacttatg atttatcaga tgggaatgga gtatgctgga ttgaacaacc tgttcacatc
      181 tatatttcaa atgttaaatt tagcggaatt gggaataggt gctgcactaa ctgtaagtat
      241 gtataaacct atagcagaag acaatagaaa agagatttgt gctttactga acgcatataa
      301 aaagtacttt tcaattattg gatggatagt gtttacatta ggattgttat gcattccttt
      361 tttgagattt ttagttaaag gttcgattcc tggaaattta aatatgacag ttttgtattt
      421 aatgtatttg ataaatactg ttttgtcgta ttggttattt tcatatagaa agagtctaat
      481 atatgcacat caaagaaatg atgtaattaa cgtgtctatg ctaattactt ttacattcca
      541 atttgtaatg caggcatatg ttctgatttt cttaaaaaat tactatttgt atttattggc
      601 ttctattttg ggacaaatag ttcttaactt aatgtgtgct gttgtttctc aaaagatgta
      661 tcctgattat actcctgttg gtgagttaga gaaagaaact attgacgata ttataaaaaa
      721 agtaaaagga ttagttacaa acaaggtagg cggtacaata ttacgatctt cagattcaat
      781 tattatttca tctttcttag gtctttcagt acttgcgatt tatcagaatt attactttat
      841 tatttctgcc ataatgagta tatttgcaat aatttatgaa tcatgtatag caggtatagg
      901 taacagtctt attgtagaaa gtcaagaaaa aaattatata gatttttgtg atattacaac
      961 aattataggt tggtttctgg caattagttg tgcatgcttt attacattat ttcagccgtt
     1021 catgattatc tgggtgggaa gtgataatct tatgagtatt tcatttgtta tatttatgat
     1081 agttcatttt ttcttatatg aaattgatca gttaattggg ttatacaaag atgctgctgg
     1141 aatatggtac gcagacaggt ttagaccatt aattacagca atgataaaca ttgtattaaa
     1201 tatcatcctc gttaattggt tcggattata tggagttttg gcggcaacca ttgtttcagt
     1261 attgttggtt gatttaccct ggttattata taatgttttc caaaatatat ttaaatttga
     1321 atcggcaggt aaatatgttt ggaagctcat aagtttgtgt gtaatagctt ttgttgcttc
     1381 ttatttaagt tattttgctt gttcgtatgt taatatcgga aatttcaaag tgataagttt
     1441 agtgataaat ttagttatat cggttgtcat atcgatttta gttattcttc tgtttaatat
     1501 ccgtaataag aagtttaagt atattttgaa agttcttacg tcggtattgg taaaaagatg
     1561 agaagagaca tcagaatgat atataaagta actacaattt ttaggctgcc gaataggcga
     1621 acgatgtata gtttttgttc caacgaagac tcttgttgaa attggagatg atatgaagga
     1681 gttacaattt tgactcatgt ttatgattga tcggttttaa aagtagtatg gagaagcttt
     1741 gggatcatcg gagaggatca aaataggtaa taatgttttt ataggaatga agtctagaat
     1801 actaaagggt gtacatatag gaaataatgt agttataggt gcaaatagtt tagtaaacaa
     1861 ggacgtatca gacgattgtg ttgttgcagg aaatctagca aaagtaatta tgtccctaga
     1921 tgaatattat aaaaagataa acaatatgaa gaatccgtaa aacttgtgaa actataccga
     1981 gaataatatg ggaaaaatat tgatgaaaaa gcattatatg agtttttagg gctgtttaca
     2041 gatggagatg acaatttgtc gaagagttgg gagcaatgca aaatttagta ggggattata
     2101 aacagactga caaggtattt aaagaacatg cacaagtatt tcataatatt gaagaatttt
     2161 tgaatactgt gagataggag ttgctacatt gagaaaagta tgtatttttc caatgtaaaa
     2221 gattacgagt ttgaaaacga caccataata ttatttttta agaagatatt atagagctta
//
LOCUS       DAZKOW010000184         2278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000184 DAZKOW010000000
VERSION     DAZKOW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.192"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..447)
                     /locus_tag="ACG912_002851"
     CDS             complement(148..447)
                     /locus_tag="ACG912_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFQ0514105.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            798..1430
                     /locus_tag="ACG912_002852"
     CDS             798..1430
                     /locus_tag="ACG912_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFQ0514106.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1553..2191
                     /locus_tag="ACG912_002853"
     CDS             1553..2191
                     /locus_tag="ACG912_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFQ0514107.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT          763 a          362 c          332 g          821 t
ORIGIN      
        1 gttaattaaa ggataaaata caatataaag aaacatctgt ttctttattt agaataaaaa
       61 aatacagact ctgttcaaat aaatgaacct agcctgcatt atgagattga ctatactttt
      121 taattcattg agatgaataa ctcgttttta taaatgccca aaagtaatcg gtgccgaagc
      181 cattcctcga cctaattctt tttcaaattt tcctattgtt gatatctcca tataaaacgg
      241 tgatactggt ttagacaatt ctcccctgat tgctaacgga cgtaaactac gttccaaatt
      301 atttatgact ttaggataat actgaccttt ttctaagcct gtctttgcgt ctatcaaaag
      361 tatacgttcc ttttctttga tctctggatt taaaacgaga tcatataatt ttagtaccat
      421 gttttctatt tcttcacttt tagacatcac gatttcctcc caaaaatata tttcttccca
      481 ctacattcta taaacaaagt atacgctaca tctagatttt tttaaatcat tactggattg
      541 ttaaaacctt tcaatacact gttatggcaa cgatttttat tttaaaaaca ttctaaatta
      601 aatcagtaaa aatttttatt ctaagatttt tattagaatt acgcctaatt tgtgtctctc
      661 agggcaattc taagaacgtt tcaagagata gcgacactat cgttatcgaa aaaggcgaaa
      721 acctatctca ttttctgcta atgtaagttg gtttagcttt tgttaatatg actttatgaa
      781 aatggaggta attaaatatg atttttccta aagttaatga agcaagcttg aataaaggat
      841 ccttttttca tatctatgaa aacgaagatt ttgaactgta taatatcctt tcacaaaaag
      901 aggtcatcga aaaccaaata atctgccatc caattattta caaatctatt ttcaagtatg
      961 cattttttgc ttccaatgat tttcaaagaa tggagattac cgactgtata ttcgaaaact
     1021 gtgatttttc aggctgtata ttgacaaaaa gcatgttgca tcgcgtgaaa tttgtcaatt
     1081 gtaaattaac aggtactaaa ttgatggaaa gttatattgg gaacacgtta ttcgagaatt
     1141 gtaaaatgga ctatgtaaat ttatctggtt caaacataaa agaaagtacc tttgagcact
     1201 ctgtcttatc aagcgcagat tttgtagact gttccctcac taaaataatg ttttctacta
     1261 atgatttaga aaatgctagc ttttttgata ctgatttaaa aaatatcgat ttaagtagca
     1321 actcctttga aatgattgaa gtacgctcgg aacatctaaa aaattgtacg cttaatcagt
     1381 tgcaagcact tggttttgct agaaaatttt tacaaataaa agtagtataa tcaaaatgaa
     1441 tatataagat aaatttttag caatacaaat atccataaaa aaacattgcc tattttaact
     1501 cttcatgtat actgactcta tcttcaaaaa ctgaatagta ggagttatta tcatgaataa
     1561 cattttagga aaaaaaatag aacttaaaaa agggatattg ggcactattg tcgttgtact
     1621 cggtttgtta gggttgctat ttgcgccatc cattattagc attttttctt taactatcac
     1681 tggatttata gcatttacac ttgtttttgg taaaaaagat acgaaaaaaa tgttctcgaa
     1741 acctgtcgct cctgtcaaaa atatagccaa atattttttt atcaatgtag taatcagtgc
     1801 cgcagtctcg gtatttttgc aacaaatttt gaaatggggt ttaacaggca atccaataaa
     1861 tgaggctttc agcccgttat tatttattat tttgcctatc atgatacttg gagaagaatt
     1921 gttctcagtg tattttctcg ctattttttc tagtaagttc agcattcctg tcgctagttt
     1981 cttgtcagca atcatctttg gatttatcca ttacagtact tatgataatg gaaatatttt
     2041 acacactgta gcacacatcc ttttgataca aggggtggca agattgttat tcaaccaagc
     2101 tgccattaaa agcaactcga tcattacaag ttgggcagtt catgtgatat ttgatttcac
     2161 agctatattg ctagtcgttt tattctccta aatttttata gaaataatac atgctctatt
     2221 gctaagaaag catactttat ttcctttaaa caaaaagaca gatgtatcaa tggattaa
//
LOCUS       DAZKOW010000185         2243 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000185 DAZKOW010000000
VERSION     DAZKOW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.196"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG912_002854"
     CDS             complement(22..837)
                     /gene="thiD"
                     /locus_tag="ACG912_002854"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFQ0514108.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            1131..1406
                     /locus_tag="ACG912_002855"
     CDS             1131..1406
                     /locus_tag="ACG912_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514109.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            1833..>2243
                     /locus_tag="ACG912_002856"
     CDS             1833..>2243
                     /locus_tag="ACG912_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0514110.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLE"
BASE COUNT          726 a          435 c          338 g          744 t
ORIGIN      
        1 ctttttcaca acttctttct tttactctaa ttcaccagct gcccaatgat tgatggggcc
       61 atatttgtgc cctacattta tttcttcacg aattgcacgg tttacatagt cttttgccaa
      121 tcggatcgct tgttcaactg tttttccttt cgctaactca gctgcaatac atgcagatag
      181 cgaatcgcca gttccgttga tccggtcggt aggataaaat ggtgcattta accaaaatga
      241 ttttcctgat tcgaacaata cgtagtcatt gactgtttct tgctgcgcat ttttagcatg
      301 atgtcctttg atcataacgt tttttgcacc catatcttgc agttcttttg ctgcttgcac
      361 aaaatcttct tcagctgcta atgtccgtcc agtcagcttt tctgcttcat aaaaattagg
      421 tgtcagtact tcggccaaag ggacaagctt ttctttcaag ctttgcaaag cagattcttc
      481 tagtaaaaga tttccatgtt tcgttacgat tacgggatcc acgactaatg gtccaaaatc
      541 atatttttgg taatttttta caactgtctc aatcaacttt gaatctgcta gcatacctgt
      601 tttagctgca cgtatttgaa agtccttcgc aatcagttca aattctttgt cgataaaatc
      661 tgttggcaat gtcacacttg ctccgattcc gtaagaatta cctgcaacac atgcggtcat
      721 gacagaaaca ccgtaaacat ttcttgtaaa gaatgtatgc atatctgctt gcatccctgc
      781 gcttccatcg ctatctgatc ctgcgatcgt cattgcttgt acatactgat cttccatcaa
      841 atcgattcct ccatttttat aaaaagattt ttcataatac tgttactgtt tctatcatat
      901 gtttttcaaa gtatccctca gtaaatatac cactaccttt tcttggtaat caaatttatt
      961 tgaaaaaaca tttgcaataa atcagaaaat agtggtcgtt tatttgtatt cgctattcta
     1021 ataataaata accactattt gatttatata tatttcagta tccttcttgt tttcatataa
     1081 ccaaaggcgc tatgatttcc atatgaaaaa aataaagtca ggtgaaaaaa atgaaacttg
     1141 aggataaaaa agtcattttg aagaaaatca aacgactacc agaccaaata gatggctata
     1201 cgatctgttc tttcaaaaaa gacaggagta taaaagctta tacgcaaaac acagaaaaag
     1261 gaataatcta tttcctacat gaagaaggtt acgataccca aacctttatg tttactgaaa
     1321 aaaaagaatt tcataaacag atgaaaaaac tgattgaaaa agaatttcca agaagtcata
     1381 aactctatgt gaatcagcaa ttttaacttc taactgttct ttattaatta aaaatgttct
     1441 gtaataaaat aaaactttat aacaactata acaaaaccga atgcaaaaca gaagttctat
     1501 aagttataat catagttgtt aggtgattca tctagtaaaa acaccagatt tcaaatttca
     1561 cacaatttaa actagatcaa tcatcaacct tcttcaaatt gttagaaccc tttcccgagg
     1621 agctgcagca taagcaaaaa aacaaacagg aagccaaatg gctaagacat tttttctatt
     1681 agtctagttg cttctcgtcg caagtttaag actttagaaa gttatttcta atgatgtttg
     1741 ggttgttttg atcaatgctt atgcacagct ccttacctat tatttatctc aacagatcta
     1801 acaaattaat ggactattta aggagttttt ttatgcttac gatctattct tctcgcacac
     1861 atagttacca tctgatggca aactggctga aagaccatca aatgccttac agagaacgaa
     1921 ttttcacacc taaacagcct ttaacaagga aagagatatt ttatttattg tcattaacag
     1981 aaaacggatt tgacgatcta ttagctaaac gctcgattga ttataaaaat ttgaaagacc
     2041 aaatcgacca atgtacgacc tcagagcttg tcgatctgat cgtctcgcat tttactttgt
     2101 tacaacgtcc catcattact gacggaaaga aattattagt cggattcaat gaagatagca
     2161 tgcgtctttt tatccctgta caatggcgca gaagtcaact gaaatctgtc tataacgatt
     2221 ttacatgggt aagcaatcta gaa
//
LOCUS       DAZKOW010000186         2227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000186 DAZKOW010000000
VERSION     DAZKOW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.197"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..280)
                     /locus_tag="ACG912_002857"
     CDS             complement(<1..280)
                     /locus_tag="ACG912_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0514111.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="ACG912_002858"
                     /pseudo
     CDS             742..2124
                     /locus_tag="ACG912_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          821 a          377 c          375 g          654 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 gtaaaaaact attgcacaat acaagtagcc tacggctatc gaaatttcca taactttcga
     2221 gcaagaa
//
LOCUS       DAZKOW010000187         2057 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000187 DAZKOW010000000
VERSION     DAZKOW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.198"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..307
                     /locus_tag="ACG912_002859"
     CDS             134..307
                     /locus_tag="ACG912_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514112.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            947..1420
                     /locus_tag="ACG912_002860"
     CDS             947..1420
                     /locus_tag="ACG912_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFQ0514113.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1429..1656
                     /locus_tag="ACG912_002861"
     CDS             1429..1656
                     /locus_tag="ACG912_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514114.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          668 a          334 c          306 g          749 t
ORIGIN      
        1 ttcggagaat taaaaaatac aataaataac tttagttact ttaatgcgtt cttactttat
       61 ttttatatta tcatttttct tattcacttc tttggtttat actattctta taaataaaaa
      121 cgaggtatgt cctatgatga aaagattgta ctattcattg atcataacga ttggctattt
      181 gattgtatca aatctaggaa atatggtatt tggtatcagt aaagaattca gttggactac
      241 tacattatgg gaatctctat tctttttcat cttcgtattt ttgttacaaa attatcgaaa
      301 aaaataaaca tgtgccatct atctgttggc acatgtttta ggatttatgg tactttttat
      361 tcggcaatcc attttatttt tctacttctt cttaatacct ctaacacttt tgatttataa
      421 actaatccaa tgcatctact tgcgcttatt aagctatttc agctagatag taccacccca
      481 tgcgtcaatg cttgattttc cttttgtata acattgtata agcaataaag gacaaataag
      541 tggtgattac accaaaccat tgaataagaa aaattctgat acgtaaataa acatgaactt
      601 gaaaaaatta atgaatcggt tagctactac gtagttgtct tttttctcat acagttctgt
      661 taacttacac ttctcttcgc tagataataa aaaatcctcc aactgtattg tccaatataa
      721 tcccaggcat ctaaactagc attatagaag agacacacat agaatctaaa ataactggtt
      781 ttaaatgttt cttacaatca aaaaaagctg ttaaatgcac tccacacctt catactctat
      841 cattttttat aaacgatata tttttattgt ttttcgataa aaaatagttg ttttacaata
      901 attattctgc tatattttag ctatcaaaga taaaagaggg ataaaaatga aatttaaaac
      961 attgctatta aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact
     1021 tttttatact cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt
     1081 atttgtttct gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt
     1141 gaaactattg aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact
     1201 aaaaaaaatc gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa
     1261 atttatgaaa gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt
     1321 cgtattcatt ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca
     1381 tgcgatttta cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc
     1441 gttaatttag atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa
     1501 gtgggcatta cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt
     1561 ttttccactc tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc
     1621 tataaaaatg actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat
     1681 aaaaaataat ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc
     1741 agatttttta tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata
     1801 caaagggggc cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt
     1861 tacgttgtag ttggatctgc ttttagtata atactttatt cattccacca gactgatagc
     1921 agtttgagca gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag
     1981 tgcttaaaac ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa
     2041 gctcccatat atactgt
//
LOCUS       DAZKOW010000188         2019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000188 DAZKOW010000000
VERSION     DAZKOW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.199"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            100..570
                     /locus_tag="ACG912_002862"
     CDS             100..570
                     /locus_tag="ACG912_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFQ0514115.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="ACG912_002863"
     CDS             792..1895
                     /locus_tag="ACG912_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFQ0514116.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          545 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatac
//
LOCUS       DAZKOW010000189         2014 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000189 DAZKOW010000000
VERSION     DAZKOW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.200"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..459)
                     /locus_tag="ACG912_002864"
     CDS             complement(<1..459)
                     /locus_tag="ACG912_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0514117.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNM"
     gene            complement(460..1368)
                     /locus_tag="ACG912_002865"
     CDS             complement(460..1368)
                     /locus_tag="ACG912_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HFQ0514118.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            complement(1368..>2014)
                     /locus_tag="ACG912_002866"
     CDS             complement(1368..>2014)
                     /locus_tag="ACG912_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFQ0514119.1"
                     /translation="LLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWE
                     DYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLII
                     EHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYD
                     EVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          679 a          348 c          226 g          761 t
ORIGIN      
        1 catattaaaa tagggattac gtcttgcaga aacaactgaa aagtagacgt ctttatctaa
       61 attttttaga tgttcatcta ttaattcttc aagattagaa acttcacgta aaggtgaagt
      121 caaatctaaa tctataatta aatcaaatat attatcttct ctttctagtt gtaagaatga
      181 atcacgtatc acttcaattt tccctacagt atctgtagct aattcaggtt tacgttctac
      241 aattttcaca gacggattta acttagtcgt caattctact aactcattac tgtccgtatt
      301 caaacaagtc gtgcaattat gttcaggatg tttttgttga tagatatcta tcattgctaa
      361 agcgaaatgg actaggggct ttcctgccaa atatttcaaa tttttatttt taaacccctt
      421 agacccagct ctaccacaaa tagtaaataa aatttccatt tatttctcct cagctatttt
      481 caatacttcg tttgcatgat ataaatcatt ccagttttct ttgcctgtta ctacgttttc
      541 aaagaaatat ttcaactcct tctcatacat atcattagca gattcaggca tttctttaac
      601 aatgttttct tcaaaactta ttttattatt taataagtca acattgatac attcatctaa
      661 agtaaacaat tgaaattttc ttgtaggaac tcttccaaaa taatctaaat gaatttctat
      721 aattttatca tcgtatctca atatatatgt tgctaaatca ttactgttaa tttcaagatt
      781 cgaatattta ccaaactgtt tttttactga tttagggaaa ccaaataggt ttataatata
      841 atccaattca tgtatacagt ctaattctac tcctcccccc atagatttaa aggcagaata
      901 attatttcta taatcagtac cttttctcca atcaggaaga taagtagaac aaatagctct
      961 tacagaatat acttttttac tttgaatact attacgaaca acttccatga tatttttata
     1021 cctcagtgga gcagcaacat aaatattttt gtaatcataa ttatattgag taagatcaat
     1081 actctcagaa aaagcaggtt tttctaaaaa tatgtaatca actttttcaa caattacatt
     1141 tagtgtttcc agatgcttaa aagtcggatt tgtaataaaa gctatatcat aatgtgtatc
     1201 caaatcgtct ctattttgaa tatacgttat ttcttcaaac tctttttttc cactgctact
     1261 ataagctgtt atatcaaaat taatagaatt cttttttcca tagcttatta gattttttag
     1321 atgtctcttt ccaattgacc ctaacccaaa aaaaattact ttcatcatta atttacctca
     1381 atgtcgttaa ttaagttaat aatatactta tctttttcaa aagtatattg gggttccatt
     1441 ttcttttcca aaagcgtgaa aaattcttta atctcctcta aatatgcatc ttcaataata
     1501 gtatgtgaat aattattatc atgcataacc tcatcgtaca attgaatatc tgtcatacta
     1561 gaagtttctt cattccatga aaatagagaa tccgggttac cttcccattc tattaaaatc
     1621 ttttcaccaa ttatagttaa atttctttta gctactcttg aaacaacatc tacacttaaa
     1681 gttccaacta taccagtttc atgctcaata ataagagtga aactgtccga ataatcaata
     1741 tcaagattgg ataatttctg ggtcattaca gtaaacgatt taactttccc aaatgtatct
     1801 ataatccaag gtaattcaat agcgaatatt tctctacacc cattagtttt tttgtttgaa
     1861 acaaaaaaat tgcgataatc ttcccaagga tgccagtcgg gtaaatactg tcctacatgg
     1921 tattgataag aaattttttt attatgcgtc actttagact taatatattg aatttctttt
     1981 ctcttcaaaa atgtagaaga tagataaagt aatc
//
LOCUS       DAZKOW010000190         2003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000190 DAZKOW010000000
VERSION     DAZKOW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.201"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..378
                     /locus_tag="ACG912_002867"
     CDS             <1..378
                     /locus_tag="ACG912_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFQ0514120.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACG912_002868"
     CDS             426..1826
                     /locus_tag="ACG912_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFQ0514121.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAZKOW010000191         1979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000191 DAZKOW010000000
VERSION     DAZKOW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.202"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(346..528)
                     /locus_tag="ACG912_002869"
                     /pseudo
     CDS             complement(346..528)
                     /locus_tag="ACG912_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(728..1744)
                     /locus_tag="ACG912_002870"
     CDS             complement(728..1744)
                     /locus_tag="ACG912_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0514122.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          556 a          460 c          310 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaacg ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgaggga tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt catgcaaaat attcgcttat
      661 cgtgaattta cgtcactaaa cattgacgca aatccactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAZKOW010000192         1967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000192 DAZKOW010000000
VERSION     DAZKOW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.203"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACG912_002871"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACG912_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFQ0514123.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACG912_002872"
     CDS             852..1040
                     /locus_tag="ACG912_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFQ0514124.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACG912_002873"
     CDS             1053..1610
                     /locus_tag="ACG912_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFQ0514125.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACG912_002874"
     CDS             1642..1884
                     /locus_tag="ACG912_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFQ0514126.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          718 a          305 c          404 g          540 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatat
//
LOCUS       DAZKOW010000193         1851 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000193 DAZKOW010000000
VERSION     DAZKOW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.204"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            205..402
                     /locus_tag="ACG912_002875"
     CDS             205..402
                     /locus_tag="ACG912_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514127.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            508..990
                     /locus_tag="ACG912_002876"
     CDS             508..990
                     /locus_tag="ACG912_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514128.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1048..1644)
                     /locus_tag="ACG912_002877"
     CDS             complement(1048..1644)
                     /locus_tag="ACG912_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0514129.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT          574 a          275 c          369 g          633 t
ORIGIN      
        1 aattagtttt taaggaatat gttttaattt tttgaaaaaa attcttctaa atacaacagc
       61 tgttttaatt gtgaaaacga aaacagtacg ctataatgcc tgtaaggttg atttttaact
      121 gtaaataaaa atcaatgagt taaatctcca aagaaatagc agtggtaatg tggagaaaag
      181 ttccagcagt ttaggaggat tttgatgacg gaagaaatga ttaatttagg agaacaatat
      241 gcatgcaaac caattggttt tacaaaaacg gtcattggtg aagttgtatc aaaaatgaca
      301 aactgcgccg tggtcaaagt tgcccaatgt gctgcagagg accaagaatt attagatgaa
      361 aaagcaagca tggttgttgc aaaatatgat acatttgaat aaagaaaaca attggacgtg
      421 cggcgtgctg caagtccaat ttctttgaga aggtaaatca gtattctttt cctattttct
      481 ggagaagaaa aagcgaggcg agttgtcatg gtaacattct ttacggttct attgttttta
      541 ttcgttgttg tgatgttttt atttctttgg ctggttcgaa aagaaattat ttatcggact
      601 gtgcgaaatc gatgggtcta cttggtgata cccttcttag tgtcactagt catttggtat
      661 acactcatca gtcatcctac agcagatgaa ttggcaaaag ggatcttgtc agcaatgatt
      721 tttattagtt ttttgttgga cagtagagga atcacagaag aaggacttgt tttgaatagc
      781 tttgacaaaa aaggtgttcc tttttctgag atcaataaaa ttgtattata tcagccaaaa
      841 ggttctaaaa tcgtaaaaat gaattttttc cgaaacggat ggcgaggacc gatgctgaaa
      901 ttttctgcat cattagaaga attagttcca tttttgtccc aacgcttaaa cgatgaggca
      961 gagattgata ttatgattga tccagaataa aaacagaggt tgcgacaaga tgtttgtcac
     1021 aacctctgtt ttttatttca aataaaatta tccaaatacg cggaagcgat cggcatcgtt
     1081 catgtattct ttttcctcag ccggtgtttc tggtgaaccg aatactaatt gcgaacgtag
     1141 tttccagttt gaagggatat tccattcttt tgcaactgct tcgtcaataa ctgggttgta
     1201 atgttgcaag tttgcgccca atcctttatc aactaatgct acccaagtat tagctgttgc
     1261 gattccgttt gattgttctg accaatctgg gaagttgtct gcatataatt caaattgttc
     1321 ttgtagacct tttaccacgt cagtatcttt gaagaataat actgtaccat aaccattttt
     1381 gaaaccagct aatttgtttt gtgtatttgg gaaagcttct gctggagtca atgggcgaag
     1441 tgcttcttca gtgatttccc aaagtttttc atgtgcatct ccgaaaagga taactgcgcg
     1501 tgttgattga gcattgaaag cagttgggct ttctttaatt gcttctttga tcaaagttgt
     1561 taattcttca ttagaaagtg aaacattacg tcctagatga tagattgaac ggcggttttt
     1621 caaagtgtca gtaaaagttg tcataataaa atttctcctt tgatttgcgt tttgcatttt
     1681 tttaattgat tacgaaatga agtatagcac cttacaaaaa gtaagtaaac agatatgctc
     1741 acaaaaaata agcgaagttc aaaaaacaat tttaataaga aaaatcgtta tgtaaggatt
     1801 ttcttagtta tcgctttcat tgttgtgttt taggaatttt tgcttatttg a
//
LOCUS       DAZKOW010000194         1814 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000194 DAZKOW010000000
VERSION     DAZKOW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.205"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(188..1207)
                     /locus_tag="ACG912_002878"
     CDS             complement(188..1207)
                     /locus_tag="ACG912_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0514130.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1204..1428)
                     /locus_tag="ACG912_002879"
     CDS             complement(1204..1428)
                     /locus_tag="ACG912_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFQ0514131.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            complement(1425..1718)
                     /locus_tag="ACG912_002880"
     CDS             complement(1425..1718)
                     /locus_tag="ACG912_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514132.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
BASE COUNT          513 a          371 c          310 g          620 t
ORIGIN      
        1 ggttgttgcc acataaagcc ataaacaatc aaccagtaga atgtgtggca acaaacctgt
       61 tatcgcatag cttggagtgt gactattatg ggtgatagtg aagatatgcg ataacatcac
      121 tattttatcg aatgattttt atagttgtca atatagttat gtaatgctcc tcaacgagga
      181 gctattttta tcgtttagga atatttaaat accaacgttt gtcatggaaa tcttgcgcac
      241 cgcctttagt gtttccctct ggatcattcg ttgcccgcat catgacatag actttcttat
      301 tagggaagtt acgcatgtta aaagatacat gatagccaac atttccagaa gtattataag
      361 cttgatttac atctgatcta taaattccat cagctcttac tcgagctaat tctttcccag
      421 tattgtaatc cataatgaag atatactcgt atttatagtt agcaatgtgc catccagcta
      481 catgcaaatt tgcattttcg atttctccaa actgatcaat gtgggcgtaa ttcgttccat
      541 ctgtcagtgt aggattagct gcaccagctc gtgttggatc aatgacaggc ttgttttcag
      601 aagttgttgg attttcatcg gtaaatccat gagctaaatc atatgctaat ttttctttac
      661 ttacgcccat ttcagaaaga taaccgtaag gatctgtatg atcgccccag atgttttgtg
      721 ttacccataa atgcgatttg attcccggtt ggttataagg agtgtccaac gttaatggaa
      781 taccatattt cattgctgaa tctctagcca attcaacgta tgccttgtag tttttctcaa
      841 acgttgcttt atcatgtgtg tgttgtaact caatctgcac aggactgttg gcattagcat
      901 acgaaccagc accgtactgt acataaccag gttgaccgac ttgataaaca attccgccgt
      961 ctcccacaat ataagcagta taagcgctag tccatgaacg ttgcatatac tgcgcttcat
     1021 tgcgtcctgt tgctgtttca ttagccgttt catgcagtaa aatatactga ttatttgcta
     1081 cttgtgagct accttcattt gcgcccaaat taaattcatt gttgatagta taggcaaatc
     1141 cgttaattgg caataaaaaa agagccgtta acaggctcat cgcagtaata gtaattttct
     1201 ttttcatttg tttcctcctt cttcgctttc agccgagaac attttgtagg ttcgatttga
     1261 tacacccaac acactcccta aaaacgcgcc aaaaccagta atgatgacaa cacagatatc
     1321 tgtgtactgc caattgagcg ctttaccaac taaccccacg aaagtagcta gtgcgggaat
     1381 aattaccagt gcgaaccatt ttagtacttc gaacgtttta ttattcattt tcttctctcc
     1441 ctaaacaaag ttttgatttg ttgcgtgtgt tccaccaatt tttctgtatg tgtatctaat
     1501 ctttcatcgt gtttctttag ttcttcatga atcatcaatc gatctgattt gctcgattct
     1561 aaatctttag tcagcaaatc taaattgtgg cttacttttg aaagagtctc agtaattttc
     1621 gagaaagatg cagcaatcgg tctaattact aataaaatca aagaaacgat agcggttatt
     1681 gatcctgcta tcattcccca ttcccctaaa ttaatcatgt gacaactcct ttaccttaaa
     1741 taaaaacgca tcaattaaga tgcgctctta tctttattaa tgattttatc tgcttcttcg
     1801 tctgtaatgc ataa
//
LOCUS       DAZKOW010000195         1802 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000195 DAZKOW010000000
VERSION     DAZKOW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.206"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..898
                     /gene="erm(B)"
                     /locus_tag="ACG912_002881"
     CDS             161..898
                     /gene="erm(B)"
                     /locus_tag="ACG912_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0514133.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
     gene            1068..1328
                     /locus_tag="ACG912_002882"
                     /pseudo
     CDS             1068..1328
                     /locus_tag="ACG912_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
     gene            1462..1701
                     /locus_tag="ACG912_002883"
     CDS             1462..1701
                     /locus_tag="ACG912_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFQ0514134.1"
                     /translation="MIVGNLGAQKEKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMEIRLRSKLKSVLIVHYLKILYNRN"
BASE COUNT          707 a          273 c          311 g          511 t
ORIGIN      
        1 aaacagacta aaaacagtga ttacgcagat aaataaatac gttagattaa ttcctaccag
       61 tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc gtttaacttc
      121 tgtatttgtt tatagatgta atcacttcag gagagattac atgaacaaaa atataaaata
      181 ttctcaaaac tttttaacga gtgaaaaagt actcaaccaa ataataaaac aattgaattt
      241 aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa cgacgaaact
      301 ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat tcaacttatc
      361 gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata ttctacagtt
      421 tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc atttaagcac
      481 acaaattatt aaaaaagtgg tttttgaaag ccgtgcgtct gacatctatc tgattgttga
      541 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      601 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      661 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      721 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      781 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccattac
      841 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt
      901 ctatgagtcg cttttttaaa tttggaaagt tacacgttac taaagggaat ggagataaat
      961 tattagatat actactgaca gcttccaaga agctaaagag gtccctagcg cctacgggga
     1021 atttgtatcg ataaggggta caaattccca ctaagcgctc gggacccctt gtaggaaaat
     1081 gtcctaagtg tggcaacaat attgtattaa aaaaatcgtt ttatggttgt tcaaattatc
     1141 ctgaatgtaa gtttacttta gctgaacatt ttagaaagaa aaaactaacc aaaacgaatg
     1201 taaaagaatt actggaggga aaagaaaccc tggtaaaagg aatcaaaaac aaagagaaaa
     1261 agccctacaa tgccgttgta aaaattgggg aaaagggata tattgatttt atatctttct
     1321 caaaataaac ataaaagccc tttaaagagg gcttttatat attaatcaca aatcacttat
     1381 cacaaatcac ttatcacaaa tcacaagtga tttgtgattg ttgatgataa aataagaata
     1441 agaagaaata gaaagaagtg agtgattgtg ggaaatttag gcgcacaaaa agaaaaacga
     1501 aatgatacac caatcagtgc aaaaaaagat ataatgggag ataagacggt tcgtgttcgt
     1561 gctgacttgc accatatcat aaaaatcgaa acagcaaaga atggcggaaa cgtaaaagaa
     1621 gttatggaaa taagacttag aagcaaactt aagagtgtgt tgatagtgca ttatcttaaa
     1681 attttgtata ataggaattg aagttaaatt agatgctaaa aatttgtaat taagaaggag
     1741 ggattcgtca tgttggtatt ccaaatgcgt aatgtagata aaacatctac tgttttgaaa
     1801 ca
//
LOCUS       DAZKOW010000196         1701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000196 DAZKOW010000000
VERSION     DAZKOW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.207"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..1636
                     /locus_tag="ACG912_002884"
     CDS             2..1636
                     /locus_tag="ACG912_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFQ0514135.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT          583 a          225 c          230 g          663 t
ORIGIN      
        1 catgaggtgg tatatgaatt tgaaaaaaat tttttctgta aaaatattta tagtttatgc
       61 atttgttagt ttattatatt tatttccagc atttaaaaac atggaaatat ttggctttta
      121 tgatacggca tttcacatta atcgtgcgtt aagtttagaa tctattttta gctcccctat
      181 taattttgaa acgtttagat catatggcat gcaagtaaat aatttttacc catggttaac
      241 tctatatcct ttatttttat tgatcaagtt tactaattta gcaataggct ataacctttt
      301 tctttatatt gttacgctca ttactctatt tatatgtcat tatgtaatgt atgaaattac
      361 caaaaaacat gttactagct cctttttcgc tattatatat acaacatctt cttttaggag
      421 tgtagaaata tttcttcgag gggcaatggg ggaattactc gcaatgtcaa tacttcctct
      481 tattttactc gggtttataa agctttacga ctcaaaaaaa gaatcttggg ttatgttggc
      541 gattagcatg actttattaa tttacactca tgttctttct gtagctatga ctatggtaat
      601 gattataatt gctttaatta ttcaatttta tcggaaaaag aaaatagaaa tcttcttaat
      661 tattaaatta ataaaagctg ctatagttac tttacttctt tcactagcgt ttataggtcc
      721 tatgattgag cagacattgt atcaagatct gaatagacct tatgtggata tattacaaaa
      781 aagagcaatt tatctgggta aagagttttt gattggaagt atagattcag aattactatc
      841 atttggtatt ggcttaactt tgcttatttg tttaattata ctaacattta actttaaaaa
      901 attaggaatc ttatctaaaa tcacttatat aatgggtata atatctattc tactttgtac
      961 aaaaatattt ccatggtttg ccttacaaaa tactccttta aatattattc aagctccttg
     1021 gcgttttatg atattttcaa cattgttcat atctttttca gtatctctat ttatttctcc
     1081 acagttggaa aaattttctt atacaaggag aagaaatttt atactattat taatattagc
     1141 tatttcaatt ttaaattatt catcagtagt taacatggtg aaagaaatga attttcaagg
     1201 aacttattct aatgaacaaa tggaaaataa acttgaagaa tttgagaatt ttgattatgt
     1261 acctataggc tataaaaaag atcaattgct tttgaaaaac catcaagtta cagaagatgg
     1321 tcaagttatt tatagtagct tttctccaaa taaagatgaa attacattta cgtttaaatc
     1381 ttcaaaaggc aaaacaatag ttttacctgt attccggtat aaaggattaa cggttactgt
     1441 taattcaaaa aatgctacag tactaaaaca aggaggtcct tttgttacag taaaaagtga
     1501 aagtggaata aataaagtaa caatcaggta ttcatacaca tttttttcta aaatctcatt
     1561 atttatttct ataatcacgc taattgttct tcttttttat tgcataaaat taaaaataag
     1621 aacaagccat gaatgatttg ctagcacata ttttagcaat ctaaagatac tattttagat
     1681 ttaataggcc ctttgtcaaa t
//
LOCUS       DAZKOW010000197         1701 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000197 DAZKOW010000000
VERSION     DAZKOW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.208"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1634
                     /locus_tag="ACG912_002885"
     CDS             156..1634
                     /locus_tag="ACG912_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514136.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          621 a          233 c          305 g          542 t
ORIGIN      
        1 tccatttttt gcaatattct caaaaaaaag ataaaaaatg agaataactg atgaaaaaac
       61 aagcataatc catcataaaa tcagtatagg tgaaaagatg aactttcatc tgcatatcaa
      121 gtaattggta tgaggcattt tttgaggtgt aggatatgaa gttggattac cttatagaag
      181 aggaagatag agaaaaactg ctgctagcta gatatttaga atttgagaaa aagaattatg
      241 tagtgctgcc taagttatat gaaacattag gtttgtctaa atttaaaaca agaaattata
      301 tcatagaatt aaataaagaa tttactgaat tcaaaacaaa gccagagatc caaatttcag
      361 aagaagcaga gatccatgtc gaacgtctga atttgacgga tatcaaaaat ttaagaagaa
      421 tctactttca acgatcaaaa attgctcgac tgttaacgga gatcgtggat tcaaaagtaa
      481 cgatcgaaaa atttgcagag aatcattttt taagcatatc aagagcctat gtaaaaagaa
      541 gagaattagt ctcttttctt gaacagcatc aaataaagtt aaagaaaaac atattagttg
      601 gggaagaaat aaacatacgg aacgtattat ttggggtata tttcggggtc tttaatggtt
      661 tttctatgcc atttagagaa agcgtcatga atgaagcaaa gagtatcgtg caatattttg
      721 aatatctgtt caatttaaaa ctgtctgcaa caaaacgaat caaattcaat ttgctaacca
      781 ctattagttt gttgagggcc tatgggaatc aaccaatcgg aaaacgtttc ttttcagatg
      841 aatactttaa gagctcagaa ggaatgaaga tcatcaagga tttatcacat atttcttttc
      901 ttcatatccc taaagagcag atcattgatg agatttctta tatcatgttg tttttgatca
      961 cggaagaaga atttgaatta gatggcgcat ttggacttgc ggaagctcat tttcatgata
     1021 tccacatgat tagtcaaaaa atgtcagtgg aattgattga tcaattacca ttttcgattg
     1081 aaatctctga agaagaaaga ctatatatac gaaaagaact tcgtatgggt atgatgcatg
     1141 tcaatcgaaa aaattggata ttcgactttg agatgactag ctttacgtct aaaaagcagc
     1201 tacagttttt cttcgagacg tatccaatat tcagtacagc aatccaaaag gtcatacaag
     1261 agaataaacg ctatttcttg ggaaaagatg aacgtttcat gattcgctta ttttatgatt
     1321 atctcttttt attggtcggt atttgtccgg tatcatattt tgaagcaccg attcatgtct
     1381 gtattgattt ttcgcaagga tctgcttata ccgattacat tatttctcaa gtgaaaggat
     1441 ttaaaaatta caatattgtg attgagagtc gaatcacttc tcgtacgaat atttatttat
     1501 ctgattgttt acttgaaaaa tttaataaag aacagataat ttggaaaaat ccaccgactc
     1561 cagatgactg ggaatatttt ggaaatacca tcatcagaga aaaaaataga catttaactg
     1621 aaaaaatcag ctagtattta tggatggcag ctttgtctca catatttatt agctgattta
     1681 tgtttttttc aaagaaaagg t
//
LOCUS       DAZKOW010000198         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000198 DAZKOW010000000
VERSION     DAZKOW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.209"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACG912_002886"
     CDS             131..505
                     /locus_tag="ACG912_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFQ0514137.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACG912_002887"
     CDS             complement(619..1248)
                     /locus_tag="ACG912_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFQ0514138.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACG912_002888"
     tRNA            complement(1382..1454)
                     /locus_tag="ACG912_002888"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DAZKOW010000199         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000199 DAZKOW010000000
VERSION     DAZKOW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.210"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(192..1484)
                     /locus_tag="ACG912_002889"
     CDS             complement(192..1484)
                     /locus_tag="ACG912_002889"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFQ0514139.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          474 a          357 c          291 g          542 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcctgaacc attgtatcaa taatagatac atatttcaaa taaaatcgaa
     1561 tgatttttat ttttatttta taaaaacgct taaaaaacga actttatctt tttagaatgg
     1621 ttattaaaaa agaggttgta acagaagtgg tcagcttcaa gaaa
//
LOCUS       DAZKOW010000200         1625 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000200 DAZKOW010000000
VERSION     DAZKOW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.211"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            97..744
                     /locus_tag="ACG912_002890"
     CDS             97..744
                     /locus_tag="ACG912_002890"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFQ0514140.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            864..>1625
                     /locus_tag="ACG912_002891"
     CDS             864..>1625
                     /locus_tag="ACG912_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFQ0514141.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQ"
BASE COUNT          551 a          207 c          334 g          533 t
ORIGIN      
        1 aagtagaaaa taatacttga aataaaagct taaatttatt ctttgtaagt tagtacagct
       61 agacaaagta tgtatccata aaggaaggag atagatatga aaaaagtaga aattttaagt
      121 aagttagaag cggctggagt cattgcggtt gtacgtggga aaaataagga tgaggccatt
      181 aaagcaataa attcaattat tgcgggtgga attaaaggga ttgaactaac ttttacagtt
      241 cctaatgctg aagaaataat caaagaaatt gctaaaaaat atgctgatca aaaagatgta
      301 ataatcggag ccggtacggt tttagatgcg tttactgctc gtatcgcaat tatggctggg
      361 gcagaatata tcgttagtcc atcttttgat cgacaaacag ctgagatttg taatttatat
      421 caaattcctt atttaccagg ttgtatgaca attactgaga taaaggaggc acttactgct
      481 ggcgtagata tcattaaact attcccaggt tctgcttttg gtccatccat tgtaaaagcc
      541 tatcaagctc cattaccaca cgtaaatata atgcctactg gcggagtaag tatagagaac
      601 ttagaagatt ggtttgaagt aggagttgtt gcagttggtg taggtgggaa tttattggct
      661 tctgttgaaa aaggtgattt tgaagaagta agtcgagtag ctagtaaata tatagataag
      721 ttttatgaga taaagaatta ttaggagaaa aaacaaataa tactctgaaa tgtaaaaaat
      781 aaatactgac tctttacgct tcagagtata gtacgtactt tcatcagaat gaaaacgctt
      841 aacaacagaa gggagagaat tgtatggaaa gaaaatttgg tataagagac aaaataggat
      901 atatgtttgg agattttgga aacgatttta ctttcatttt tgctagttct tttttaatga
      961 tcttttatac aaaagtattg gggataagcg gagctgccgt tggaacatta tttttagtag
     1021 caagatttat cgatgcagtt actgatgtaa ctatggggcg tattgttgat agatcgaaag
     1081 caactaagca gggtaagttt aaaccgtgga ttttaagaat gagtggtcca gtcgctttag
     1141 caagtttttt aatgtatcaa acttctatga tttcagcttc tatgagcgtt aaggtagtat
     1201 acatgtatgt tacgtatata ttatggggga gtatttttta cacatctata aatattcctt
     1261 atggatcaat ggcatcagct attacttcta ttacagaaga aagaacaatg ctatcgacat
     1321 tccgaaatgt tggagctact ttagcaagtt tggtcattgg tgtattaaca ccattattta
     1381 tttatacaag agatgattca ggcgctcaaa tagttcgtgg tggatcaact tttacaattg
     1441 ttgcgggaat attttcgata tgtgcactta tttgttattt aatatgttat aaactaacta
     1501 ctgaacgagt aagaattgac caatctcaat acaaagtttc attgttaaaa tctttaaaag
     1561 ggttagttaa aaatagggca ttactttcta taattggtgc ggctattttc ttactccttg
     1621 ctcaa
//
LOCUS       DAZKOW010000201         1594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000201 DAZKOW010000000
VERSION     DAZKOW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.212"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            288..695
                     /locus_tag="ACG912_002892"
     CDS             288..695
                     /locus_tag="ACG912_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514142.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="ACG912_002893"
     CDS             709..1110
                     /locus_tag="ACG912_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514143.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="ACG912_002894"
     CDS             1112..1483
                     /locus_tag="ACG912_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514144.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          596 a          201 c          277 g          520 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt
     1501 tgtttgttat ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat
     1561 atagtcataa cgccgatgat cgctacgtgc gtcc
//
LOCUS       DAZKOW010000202         1540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000202 DAZKOW010000000
VERSION     DAZKOW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.213"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1490
                     /locus_tag="ACG912_002895"
     CDS             <1..1490
                     /locus_tag="ACG912_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFQ0514145.1"
                     /translation="QQQTIQNLTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNL
                     FSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGS
                     QMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSL
                     GSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGER
                     LRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPK
                     TFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQ
                     LDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACK
                     LRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAE
                     LNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          520 a          280 c          318 g          422 t
ORIGIN      
        1 cacaacaaca aaccattcag aatcttacaa ctgaaatcca actattgaat gaaaaggtca
       61 attatttaac caacaaatta tttggacgct ctaaagagac tctctttgag gaaacgaatg
      121 ggcaattgaa tctttttagt gatgaagaaa tttctgtaag tgttccagaa gcagctgcca
      181 ccattattcc agtaaaagga catcaacgag ttgtaggaac gaaagcagac aaaatcaaac
      241 acttacctat aacagagaaa gaacatctcc ttcctttgga agaacaattt tgtgaacatt
      301 gcggttcaca gatgaaagat atcggacgga caaaagtacg agaagaaatt cgtttccatc
      361 aggctatgtt ggattgtctc acccattatc aacataccta ttgttgtaaa agctgcgaaa
      421 aagaaggact ttcttctttt aaaaaagcga tcgttcctaa acctttgatt tctaatagct
      481 taggttccaa tagtttagtg gcggaaacga tccggatgaa atttggtcag aaagttcctg
      541 cctatcggca agagaactat tggaaacaaa cacatgggtt ggatatttcg agagataata
      601 tcaccaattg gcatattaaa gcagtgcaaa acgcgttaga ccctttagga gaacgattaa
      661 gagtctatct taatcaagaa gagattctac acggggatga aacaagttat cgtgtgattg
      721 aaagtgccaa aacagatacc tattactggc agttttgtac aggaaaagac agtcagcatc
      781 ccatcgttta ttatcaccac gacgaaagtc gtgctggaga tgttccgaaa acttttctca
      841 aggagtttac agggtattta cactgtgacg gctatagcgg gtataatgcc gtggaaagtg
      901 tacgacttgt ctattgtttc gcgcatgttc ggcggaaatt tttcgaagca attccaaaag
      961 gaaagaaaaa cacggatatt cccgcagctc aagcagtcaa acaattggat aaatggtttg
     1021 tcctagaaaa gaaatggaaa gacttttctc ctgagaagcg attaagctgt cggcaacaag
     1081 aactgcgtcc attatttatt gctttttatg agtggatggc aacgatagat cctgttgcaa
     1141 aatccaagtt ggatgcagcc gttcaatatg cgtgtaaact acgaagtggt tttgaaccta
     1201 tttttgaaga tggtcgttta gaactcacaa ataatcgtgc ggagcgaaat atcaaagagc
     1261 tggtgatagg acggaaaaat tggttgcatt ccacaagtct tgaaggagca cgaacttcag
     1321 gaatcattct gagtgtatat aaaacagcag aacttaacgg attgaatcct gtaaagtatc
     1381 tagagtttct atttgataag ataccaaatc ttcccgtact ttcagcagaa actttagatc
     1441 aactgttgcc ttggaacaag gatgtacaac aacatttttc tcgtaactaa aaacgccaaa
     1501 cattcccgat tctattctat aggtcgggtt atttgacgta
//
LOCUS       DAZKOW010000203         1536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000203 DAZKOW010000000
VERSION     DAZKOW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.214"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            20..1486
                     /locus_tag="ACG912_002896"
     CDS             20..1486
                     /locus_tag="ACG912_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFQ0514146.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          421 a          289 c          326 g          500 t
ORIGIN      
        1 aagaattggg gtatagatta tggaaaaaca gcaagacaag ggcttcttcg ggcagccgaa
       61 gggattgtca actttgttct tcactgagat gtgggaacga tttagttatt acggtatgcg
      121 tgcattactt atctattata tgtacgatac agttgccaac gggggacttg gattaccacg
      181 agcaacagca ttagctatca tgtcgatcta cggatctttg atctacatgt ctagtatcgt
      241 cggcggatgg gtatctgacc gagtgttagg agcaaaacgc actgtattct tcggtggagt
      301 tttcattatg cttggccaca tcgtcttggc tttaccattt ggtgtaagta cattgtttgt
      361 ttcgatggca ttgattatta tcggaaccgc ctttttgaaa ccaaatgttt ctggcatggt
      421 cggacatctt tattcaaaga ctgacttgcg aagagatgca ggattttcga ttttttacat
      481 gggtgtcaac ttaggggcat tattagcacc actaatcgtt ggtacaatcg gtcaagaata
      541 taactatcat ttagggttct ctttagctgc tattgggatg ttctttggat tattacaata
      601 catcatccaa ggacgcaaga cattagacgg agtgggtaca acaccgccga atcctttgac
      661 aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc gcacttgttg ttatcgctgc
      721 agtattcggc ggggcacaac taactggtca tctgacaatt gacttttttg tcaatacaat
      781 cagtgtattg gggattatct taccgttgta ttactttatc aaaatgctga cttcaaaaga
      841 tgtaactgct gaagaaaaac caagagtatg ggcttatatc ccattattcc ttgcagcaat
      901 cattttctgg tcattacaag aacaaggttc atctatctta gctttatttg ctagtgaacg
      961 tacacaatct acgctatttg gtgtaacgat ccaagcaagc tggtatcaat cattgaatcc
     1021 aattttcatc tttattctga caccagtatt cgtaacgatg tggacgaaac ttggtgaacg
     1081 tcaaccatct acagtagtga aattctctct tggactttta tttgcaggat tatcgtactt
     1141 gctattgatg ttgccgggta tgctttatgg tacaagtgga aaagtaagtc cattgtggtt
     1201 gttgggaagt ttcttcatcg tggttattgc tgaattgtgt ctttcaccag ttggtttgtc
     1261 tgtgacaaca aaattagcac cacgagcatt tgaatcacaa acaatcgcta tttggttctt
     1321 agctgatgct tcttcacaag caatcaatgc acaaattgca cgcttctaca caccgggaac
     1381 agaatctgct tacttcggta ttatcggtgc agtagcaatc gtcggtggta tcttgctatt
     1441 tactgtgaaa gaaccaatca aaaaactaat gggctctatt cattaatcaa tgaacataaa
     1501 aacaggaatt gcttatttgc agcagttcct gttttt
//
LOCUS       DAZKOW010000204         1509 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000204 DAZKOW010000000
VERSION     DAZKOW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.215"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(480..1094)
                     /locus_tag="ACG912_002897"
     CDS             complement(480..1094)
                     /locus_tag="ACG912_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFQ0514147.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          493 a          286 c          244 g          486 t
ORIGIN      
        1 ataataaaca cttataaata tccattatct atccttcaat ttttgtgtat tttatttcta
       61 ttagaaaaag gtatcctgtt ggacaatttt gttatatcag gtaaacttca ttgcaataat
      121 gataacaaat gtactataat tactttgtaa agagttttgg ggaagactct ttcatcaacg
      181 tgtattaaga gacaagaaag aatccatatg gattctttct tgtctctttt tccactactt
      241 tttatttttc tgacactaca tattcttata actcatttct cataactgtg aaacaactta
      301 taaaaataac aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata
      361 actaaaacaa aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa
      421 acgctccatt tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac
      481 tattttccac ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc
      541 aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc
      601 aagactgcat catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat
      661 cgcaaaccac tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg
      721 attaatggat gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt
      781 ccttcattcg aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt
      841 gcctgtcctg caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata
      901 cttggtacaa gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg
      961 ctagcatata tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct
     1021 tgaagcatca gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa
     1081 taacataaac tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc
     1141 ctaatctaag gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct
     1201 gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa
     1261 acaacgtgca gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt
     1321 acaataaatt aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg
     1381 tgtgtgactg gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta
     1441 ttcagctgtt tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaaa
     1501 atacaaaaa
//
LOCUS       DAZKOW010000205         1485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000205 DAZKOW010000000
VERSION     DAZKOW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.216"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(69..141)
                     /locus_tag="ACG912_002898"
     tRNA            complement(69..141)
                     /locus_tag="ACG912_002898"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(106..108),aa:Gln,seq:ctg)
     gene            complement(187..615)
                     /locus_tag="ACG912_002899"
     CDS             complement(187..615)
                     /locus_tag="ACG912_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514148.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(675..1433)
                     /locus_tag="ACG912_002900"
     CDS             complement(675..1433)
                     /locus_tag="ACG912_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFQ0514149.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          423 a          284 c          276 g          502 t
ORIGIN      
        1 tacaataatc cccctgcaaa aagattgttc ttttacaggg ggttcatttt ttcagcaagc
       61 acatcaaatt gggatggcag ggttcgaacc tacaattgct tgattcagag tcaagtgcct
      121 taccagtttg gctacatccc atttattcaa caaaataatc atagcacttt cctaaaaaaa
      181 cgcaagttat ttttcttgat cggatagttg ttgctttact cgtaaaaggc ggcgtactag
      241 tttcttttct aatccagcag gaagggcaat cagttctgct actgacaaga cggtgtccaa
      301 taactgatat tctttgacag gttcattcat aatatgatag atgtttgcta aacgttcaat
      361 atattcaaag cgagtatact ctgttcgagc aattagaaat ttcaataaat cggtggcttt
      421 ctcgtactct tgatctgcgt aataaacaag agattgacga tacaatttga tttccagttc
      481 attgagggta gcaggtctgc gacgctcgtt ccactcatta atagagactt taccttgaac
      541 atttcccttg cagtcacgga caaaaataat cggaatttca tttggatcca tttttttatg
      601 tgtgaatttt tccattatct acgctccctt caaaggtttg gtatcttgag tataagggat
      661 ttttgtttga attactagct tttctccaaa gttagcatct gtccacccat tgcagtgaaa
      721 atcgccgaaa aatcgttgat cggtactgct cgatttttta caccataggg atcgttgaca
      781 taaatatttt ctttgtcata tccagtgact acacaagcat gaacaagagg agtgactctt
      841 acttctccat tatttgtggt ccaagtcata aaatcagttt catctgggac ttcaaaattg
      901 atagtcacag aagtccagac aggatttcct ttagatagtg cttttttgat atcatcgagt
      961 tgagcatcgg tttttggaaa agtgtgataa tccgtttgga cgatttgctg agcaagggag
     1021 gcaactgggg gaacatagac gaccatagcc cagtctccgc cactgatatc accaacaaat
     1081 ccttcattag gatcgccatg agtcccatct gcattgaaaa cagggacgta atccaattgc
     1141 tcagctaact ggtttttagt tgtttcataa ccataaaaag ataaaagcat agaaagcgaa
     1201 gtgacttcac agccattttc taaagaagga gaatcaaact ggctctctaa tggaacatct
     1261 agtaaaactt ctgctttctc ttttgtcttt ttcgtttttt gtgttgaact tttttgtgca
     1321 gtgttcattg tatgttctgg agaatggata tgtaggtaat aacttgtacc gcttaataaa
     1381 agagcagctg tgccccctag aaacaatagt tttttgaacg tttttttctt catttgaaat
     1441 ctcccttttc agctgtttat ctttcctatt ataaatgtat tttta
//
LOCUS       DAZKOW010000206         1482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000206 DAZKOW010000000
VERSION     DAZKOW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.217"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG912_002901"
     CDS             <1..828
                     /locus_tag="ACG912_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0514150.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETKIVDA
                     KKGKINYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESDYLAWF
                     ESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRATLFTIE
                     YGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1448
                     /locus_tag="ACG912_002902"
     CDS             828..1448
                     /locus_tag="ACG912_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514151.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          529 a          212 c          337 g          404 t
ORIGIN      
        1 aatacagagg ttatggaagc gactatagtt gaaaatggtc ttccctacga tttatcagaa
       61 tgtaaggtat attttgaatc agttgtaggt gggaaatatc cagtccaatt agaaacaaaa
      121 attgtggatg ctaaaaaagg gaaaattaac tatatttttg ataaatattc catgcagtgt
      181 ttacatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cttattttgt cattaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caatcgaaga tttaattgcg gatatgacgg actttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgaacgttg gagacaagaa
      421 caggaacaca gtttccaaga ttggagagaa ggacaagaat cagactattt ggcatggttt
      481 gaatcaatca aggatatttt aaagacgatt gatccaggtg gagtcatgtt agccgaacta
      541 acggatgcgc gagtagatat tcaaggagtt cgtcatgcgt ctatttcaga gcgtttgctg
      601 gcagatatgg actatttata tcagaaattg cgagcaacgc ttttcacgat tgaatacggg
      661 gagattgaag tgactgatat tttgcaggat gatctctttt cagataatca tgaagtcgaa
      721 aaagttggaa ctgtagcatt cccgattgaa gaaggagcct tgatcatcgc aaccgttgat
      781 gatccaaagc aaaatgtgtt cactcttgag aaagttgggg tgatctaatg gctaaaacta
      841 aacgaatgat ggaaacggat gaaaaaacgg gtgtacaacg ccaattcttt ccaatcacac
      901 atgcttccgc agttcttggg ttagaagaaa taatagcagg agaagcaaca gttttatctg
      961 ttaatggaaa aattggagcc gtcgtcatta ctaaagaaga tttgggatta gagaatgttc
     1021 tcacagaatt accctatgca agtgaagaag atgacggcat tatcacggca gaaatgtatc
     1081 aaaaaatttt aaacagtgga gaaggtgact acgtgttgcc agttgccact atcgaccgtt
     1141 tgggtggcat aaaagttggt gaattattga cgattgatga gacagggaaa gtctctgcag
     1201 tcagacaatc tgatgtcaat ttttcgctgg agttaaaaga aaaactcgat tcactgaaaa
     1261 attatactgc tggagaaaac atcactattg atgaagatgg aacaattagt tctacaggtg
     1321 gtagtgggac tggtggtgtc aatcagtcat atgtagatca aaaattccaa gaagctgtaa
     1381 atcaagcaga aaattacaca aacgaaagaa ttccaaattt tacttttgaa aaaattgggg
     1441 aggtatagag aatgacagat atcgtaaaaa taaaacaaag ca
//
LOCUS       DAZKOW010000207         1479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000207 DAZKOW010000000
VERSION     DAZKOW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.218"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..828
                     /locus_tag="ACG912_002903"
     CDS             <1..828
                     /locus_tag="ACG912_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFQ0514152.1"
                     /translation="NTEVMEATIVENGLPYDLSECKVYFESVVGGKYPVQLETRIVDA
                     KKGKIKYIFDKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGEMGSYW
                     QSIEDLIADMTDFINENKGDFTDWMNERKEEFDRWREEQENSFQDWREGQESDYLSWF
                     ESIKDILKSIDPGGVMLAELMDARVDLQGVRHESISERFLSDLNYLYQKMKAALFTIE
                     YGEIEVTDILQDDLFSDNHEVEKIETVEFPIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            828..1445
                     /locus_tag="ACG912_002904"
     CDS             828..1445
                     /locus_tag="ACG912_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514153.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          544 a          192 c          311 g          432 t
ORIGIN      
        1 aacactgaag ttatggaagc tacaattgtc gaaaatggtc ttccatatga tttatcagaa
       61 tgtaaggtat attttgaatc agttgttggc ggaaaatatc cagtccaatt agaaacaaga
      121 attgtggatg ctaaaaaagg gaaaatcaaa tatatttttg ataaatattc catgcagtgt
      181 ttgcatcgac aaacagccaa tttcattata tttaaaggag aagacttgat tggaacaact
      241 caagacttct cctattttgt catcaatgct gtttcaaaaa cagaaggaga aatgggttct
      301 tattggcaat caattgaaga tttaattgct gatatgacag attttattaa tgaaaataaa
      361 ggcgatttta cggactggat gaatgaaagg aaagaagaat ttgatcgctg gagagaagaa
      421 caggagaata gtttccaaga ttggagagaa ggacaagaat cagattattt gtcatggttt
      481 gaatcaatca aggatatttt gaagtctatc gatccaggtg gagtaatgtt agccgaatta
      541 atggatgcac gtgtagatct tcaaggtgta cgccatgaat cgatttctga acgtttccta
      601 tccgatctaa attatttgta tcaaaaaatg aaagcagcac ttttcacgat tgaatacggt
      661 gaaattgaag tgactgatat tcttcaagac gatctctttt cagataatca cgaagtcgaa
      721 aaaattgaaa cagtagaatt tccaattgaa gaaggagctt tgattattgc aactgttgat
      781 gatccaaaac agaatgtttt cactcttgag aaagttgggg tgatttgatg gttaaagtaa
      841 agagaatgat ggagactgat gaatctggtg ttaaacgtca atttatgcca attacacatg
      901 tttctgcagt tttaggctta gaaaaaatga tatcaggaca atcaaaagtt ctttcggtta
      961 atggtaaata tggtgcagtc attttaacga aagctgattt aggattagag aatgcaatta
     1021 ctgaactacc ttatgcaagc gagacaagcg atggtattct tactgctgaa atgtttcaaa
     1081 agatcgtaaa tggagaaggt ggaacatata ttttgccgat tgctactcct gaacaattag
     1141 gaggtataaa agttggggaa ctcttagaag ttactgaaga aggtgtttta tctgcaacta
     1201 agcaaacaga cttcaatttc tctgaagaat taaagggaaa attagaatct ctaaaaatat
     1261 taaaagcagg tgcaaatata tcaattgcag aagatggaac aattagttct acaggtggta
     1321 gtgggactgg tggtgttaat cagtcatatg tagatcaaaa attccaagaa gctgtaaatc
     1381 aagcagaaaa ttacacaaac gaaagaattc caaattttac ttttgaaaaa atcggggagg
     1441 tatagaaaat gacagatatc gtaaaaataa aacaaagcg
//
LOCUS       DAZKOW010000208         1417 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000208 DAZKOW010000000
VERSION     DAZKOW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.219"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(150..1337)
                     /locus_tag="ACG912_002905"
     CDS             complement(150..1337)
                     /locus_tag="ACG912_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFQ0514154.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DAZKOW010000209         1397 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000209 DAZKOW010000000
VERSION     DAZKOW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.220"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            12..422
                     /locus_tag="ACG912_002906"
     CDS             12..422
                     /locus_tag="ACG912_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0514155.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            436..792
                     /locus_tag="ACG912_002907"
     CDS             436..792
                     /locus_tag="ACG912_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFQ0514156.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          511 a          209 c          263 g          414 t
ORIGIN      
        1 ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa
       61 gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt
      121 taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt caagatatta
      181 tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa
      241 agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg
      301 atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg
      361 aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat
      421 agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg
      481 aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa
      541 cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa
      601 cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta
      661 ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg
      721 attattatcg atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc
      781 ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac
      841 agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta
      901 tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa
      961 aatatattga catgtgtaaa tttacatgat atattcattt ctgttgtttt aagtagcaaa
     1021 gcaataaaat agtattttaa aaaacatata aaatagttgt tgacatgtta gaaatgacat
     1081 gataagataa ctgagttgtt aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa
     1141 acttcaaaaa agttcttgac aaataacaaa tgataagtta tgataaatga gttgctgtaa
     1201 gatttcgaaa aacgaaatca aaaaaacttt taaaaaagag ttgacaatta cttgtctctc
     1261 tgatatgata taaaagttgc tacggcagcc aaaaacgaat cggggaacac tcctgatgaa
     1321 gtagaccttt gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg tcttgattca
     1381 attcaagaca acaaaca
//
LOCUS       DAZKOW010000210         1364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000210 DAZKOW010000000
VERSION     DAZKOW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.221"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1364
                     /locus_tag="ACG912_002908"
     CDS             82..>1364
                     /locus_tag="ACG912_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFQ0514157.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAZKOW010000211         1267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000211 DAZKOW010000000
VERSION     DAZKOW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.222"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..231
                     /locus_tag="ACG912_002909"
     CDS             <1..231
                     /locus_tag="ACG912_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFQ0514158.1"
                     /translation="TPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            236..1183
                     /locus_tag="ACG912_002910"
     CDS             236..1183
                     /locus_tag="ACG912_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFQ0514159.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          414 a          182 c          263 g          408 t
ORIGIN      
        1 acacctgaag tttttgaaat gaatgcctca atgtattctt ttaaacctga atttctaaaa
       61 aaaagtaatt atatttttga tgggcaatgt gggataataa aaatgagaga ttatctaatt
      121 ttagatattg atagtgaaga agattttaaa tggttagaat ttttgtttcc taaattttta
      181 gaagattctt cggagcttca agatatctat aattatttat atcggaggta aaaggatgag
      241 ctgttatctt atcaccggtg gggctggctt cattggttct accttagcca atcatttagg
      301 agaagaacat gtggtaatcg tcgtcgatga cttatcaatg ggaaaagtag aaaatcttga
      361 tatgacgaaa aatatcactt ttattgaggg agatgtcgct gagaaatcct tgatggaaga
      421 gatcatgaaa gctaataaat ttgattatat ctttcattta gcagcagttg ctagtgtggc
      481 agattcagtt gcccgaccag tagaaacaca tcgtgtgaat tttgaaagtg tattaatgat
      541 tttagaattg atccgcaagt atcaacctga tttaaaacgg atcgtgttta gttcttcagc
      601 ggctgtttat ggggatgaac cgactttacc aaaaaaagaa gagtccgtga ttcgcccatt
      661 gacgccttat gcggttgata aattcgctgc agaacaatat gtgctagatt attgtcattt
      721 atatgatgta cccggtagcg cagttcgttt tttcaatgtc tatggtccaa atcaaaatcc
      781 gaattctcca tacagtggtg tgatttctat tctcgtagat cgctataaga aacaaatcgt
      841 aggagaagca acttcattta cattatttgg tgatggaagc cagtctagag actttgttta
      901 tatagatgat gtaatccaag cattactttt agttgcgaat gaggaagctg cactggggaa
      961 acaattcaat gttggaacgg gtaaagcaac cacacttttg gaattgattc atgcaatcaa
     1021 tgaaattctc ggtgtagaat tgacacttga atacaaagaa gagcgttcgg gtgatatcca
     1081 tgactctcta gcagatatta ctaagatcca gtcaattggc tatcaaccga aatatggtat
     1141 tttaagtgga atgactaatt atttgaaaac tgaaatcaat taaaacatac taagataaga
     1201 ttagagggta aattcaggct ctttgtcaat aaggactgat gagttttgta aaatcaaatc
     1261 tgggata
//
LOCUS       DAZKOW010000212         1263 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000212 DAZKOW010000000
VERSION     DAZKOW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.223"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..837
                     /locus_tag="ACG912_002911"
     CDS             <1..837
                     /locus_tag="ACG912_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFQ0514160.1"
                     /translation="SFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIV
                     MNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNK
                     RKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKA
                     KAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADT
                     ISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSR
                     DH"
     gene            870..>1263
                     /locus_tag="ACG912_002912"
     CDS             870..>1263
                     /locus_tag="ACG912_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HFQ0514161.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKE"
BASE COUNT          490 a          136 c          230 g          407 t
ORIGIN      
        1 tctttttcta tagttcacaa tcaggtttat tttggcgggg gaattaattt atggttagcc
       61 aaaaagaatg gaatcagaca gagaattgct catagccatg caactgaaga tggaaaatct
      121 caaaatattg taatgaatgt attgcgtaaa tttttaacca aattgttgtt acaaaatgca
      181 acagactacc ttgccgttag tcaagaagct ggtgaaagtc tgtttcaaaa ccaccccttt
      241 gagatagttc ataatggtat tgatttagag ctttatagta aaaattcaga agctaaggtt
      301 aataagagaa aagaattgga tatctcaatg agtacttttg ttgtgggaaa cattggacga
      361 ttagagaaac aaaaaaatca atcttatttg ttagagattt ttttagaaat actaaaaatg
      421 aattctgatt cttacttgat tttagtaggt gaagggtcac ttagaaataa actgaaagcc
      481 aaagcagtag aattggatat agagaaaaga gtaaaatttt taggagaaag aaacgatatt
      541 cccgagttat tatctgtgat ggatgttttt gtaatgcctt ctctttatga aggtctgcca
      601 atggttggat tagaagcaca agcagcaaaa ttaaaattag tcttagcaga tactatttcc
      661 aaagatacaa agttaacaaa taatgtggat tttgtatcat tagatgaaac accacaagaa
      721 tgggcaaaaa aagtaataaa agagccgtat gctaataaaa taacacaaga actaaagagt
      781 tatgatgttt cgtacactac tgaacaaatg gagagtatat atagtagaga tcattaaaaa
      841 tgttcaagat attttaggag tgattatttt tgaaaatatg ttatttgata ttggcataca
      901 atgatattgg caacttaaag agattggtca atcgattgaa cgaaaatgca gtttttatta
      961 ttcatataga taaaaaaact gatatagaac catttattga agcatttaaa tcatatgata
     1021 atgttgaatt tttatgtaat aaaaaccgtg ttaaaattta ttggggtggt ttttccattg
     1081 tacaagcaga aatcaattta gtaaagagag cattacaaaa tgagaaatat ttaaaatatg
     1141 tattattatc tggtgctgat tatccaatca aagataatga gtatatttat aattatttta
     1201 aaaagaatag ttcagtggag tttattcgtg gaatagatct agatcaaatt aaacataaag
     1261 aat
//
LOCUS       DAZKOW010000213         1233 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000213 DAZKOW010000000
VERSION     DAZKOW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.224"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..340)
                     /locus_tag="ACG912_002913"
     CDS             complement(41..340)
                     /locus_tag="ACG912_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0514162.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(346..576)
                     /locus_tag="ACG912_002914"
     CDS             complement(346..576)
                     /locus_tag="ACG912_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514163.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(835..948)
                     /locus_tag="ACG912_002915"
                     /pseudo
     CDS             complement(835..948)
                     /locus_tag="ACG912_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          430 a          206 c          182 g          415 t
ORIGIN      
        1 gataaaatcc gttccgggct acctttttag gtagcctact ttaatctttg tatctttctg
       61 gatcaacgaa agtatacttt atatagtcat aacgccgatg atcgcttcga gcgtctagca
      121 catcagtcac gatatcaaac aaaaaatata cgtctttctt cattctagtt ttcgcagcag
      181 ggattttaaa gtagttctta ttagaatagt agagattaat taataagcta tcttcgattg
      241 ctaaaaagaa aacttctgaa tcccacacct tataaaaatc tttgacaaat ctattcgaag
      301 gatcaaattt aaaccataat tgcgtctcat taaaaagcat aaccattact ccgatctatt
      361 tttaaactta gtctctacct ctaatatata tcgagttttt attttgcctt cagagaatac
      421 tgtttctttt tttgcagtta caggttgttt attttcggaa aaagctaata tagctaaaat
      481 tgaaacatcc atctggaatt tgtctttttc gttgctttgc tcataaaaat ctgcatattc
      541 atcactaata ttttttctaa taaattcttc catcataaag atcacctcga aaagagtata
      601 cgaacaaacg ttctttttgt aaaggtggaa tttattatac taaataaata ggtgaacaag
      661 tacttgtgcc aaattatgtg ccaaaaaaaa tcgaatttaa taaaacacaa acaaaaagaa
      721 atcctattat gataacattt cttataataa caaacacaac agaagacgtg taatagttag
      781 tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt ttgtataagt
      841 agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac
      901 tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccatcaataa acagtcgaag
      961 agaaaatagg gaaaaattga ttactaaaga aaaacttact aggtcgacgg tgtagttgat
     1021 tttcatactt caatagattc gaattaaacg atttcgattg gcttatacca gtcctatcca
     1081 acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg taaagccaat
     1141 gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg ttaatactta
     1201 caactttcct tttcaaagta ccatataaaa aaa
//
LOCUS       DAZKOW010000214         1231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000214 DAZKOW010000000
VERSION     DAZKOW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.225"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..459
                     /locus_tag="ACG912_002916"
     CDS             <1..459
                     /locus_tag="ACG912_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514164.1"
                     /translation="AKYLKTHVEAIDGKEALVQTDGDQAIAGHKNFSGSVTINNKRVL
                     TTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGNDSVILTVPEGYR
                     TPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            473..904
                     /locus_tag="ACG912_002917"
     CDS             473..904
                     /locus_tag="ACG912_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514165.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            906..1043
                     /locus_tag="ACG912_002918"
     CDS             906..1043
                     /locus_tag="ACG912_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFQ0514166.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
BASE COUNT          449 a          179 c          257 g          346 t
ORIGIN      
        1 gctaaatact taaaaacgca tgtagaagct attgatggta aagaagcatt ggtgcagaca
       61 gatggagacc aagccattgc aggacataag aatttttcag gttctgtaac aatcaataat
      121 aaacgtgttt tgacgactga tgataacaga tatgaagtgg taaatctggt tgtcactaac
      181 ggtaatacag ggacagcaaa gctttatcgt gaaggaaaaa cagtttctat atattttgtg
      241 gctttaaacg gaaaaagtag tggcgggaat gattcggtta ttttaactgt tccagaaggc
      301 tatcggacac caattagttt tgaacaactg gttgggtcaa tagaccgttc tactttgaac
      361 agtgctcaac tatctattgg cgcagacgga gccattaaat ggagaagaaa ttcaagttat
      421 ggatcagctt attcatttgt tatcacttat tcaatttaag ggaggaaatc taatgaaagt
      481 agtttacaaa tcaatcaagc cttacggatt cgagcaaatc attttgaaca atcaagaaaa
      541 tatccctgaa aactgtacag agattaaacc accagttcct aactggagac caagatttga
      601 ttttgataaa aaacagtggg ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt
      661 tgatagcgta gatgaattgg ctaatattaa ggcattatac gaaacgctga aggcagaaaa
      721 tgatgaattg aaacagctaa attctaaagc aatgctaaac aatgtagcaa tcaagcaaga
      781 aaatgtctta ttgaaagaaa agtcagaaag tttagcgcag ttgaattcaa aaacgatgct
      841 tgcttctgta caaaatacca aggaaatcga agaaatcaaa aaacaattac aaggtgggaa
      901 gtaacatgta ttcatatgaa gatatcaaac taatgtatga ctggggctgt ttcactaacg
      961 aacaagtaat ggttttcgtt ccgttgtgca ttactgaaga agaagcagat aaaatcatta
     1021 gcaaagaaga gagcgcatct taattgatgt gctttttatt ttgattcaag gagttgtcac
     1081 atgattaatt taggggaatg gggagcgata gcaggatcaa taaccgctat cgtttctttg
     1141 attttattag taataaaacc aattactgca tctttctcga agattactga gactctttca
     1201 aaagtaagtc acaatttaga tttgctgact a
//
LOCUS       DAZKOW010000215         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000215 DAZKOW010000000
VERSION     DAZKOW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.226"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            414..947
                     /locus_tag="ACG912_002919"
     CDS             414..947
                     /locus_tag="ACG912_002919"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFQ0514167.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DAZKOW010000216         1165 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000216 DAZKOW010000000
VERSION     DAZKOW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.227"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(82..171)
                     /locus_tag="ACG912_002920"
     tRNA            complement(82..171)
                     /locus_tag="ACG912_002920"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(133..135),aa:Ser,seq:tga)
     gene            complement(195..268)
                     /locus_tag="ACG912_002921"
     tRNA            complement(195..268)
                     /locus_tag="ACG912_002921"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(232..234),aa:Ile,seq:cat)
     gene            complement(282..355)
                     /locus_tag="ACG912_002922"
     tRNA            complement(282..355)
                     /locus_tag="ACG912_002922"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(319..321),aa:Met,seq:cat)
     gene            complement(383..455)
                     /locus_tag="ACG912_002923"
     tRNA            complement(383..455)
                     /locus_tag="ACG912_002923"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(420..422),aa:Ala,seq:tgc)
     gene            complement(475..548)
                     /locus_tag="ACG912_002924"
     tRNA            complement(475..548)
                     /locus_tag="ACG912_002924"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(512..514),aa:Pro,seq:tgg)
     gene            complement(558..631)
                     /locus_tag="ACG912_002925"
     tRNA            complement(558..631)
                     /locus_tag="ACG912_002925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(595..597),aa:Arg,seq:acg)
     gene            complement(642..727)
                     /locus_tag="ACG912_002926"
     tRNA            complement(642..727)
                     /locus_tag="ACG912_002926"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(691..693),aa:Leu,seq:taa)
     gene            complement(744..815)
                     /locus_tag="ACG912_002927"
     tRNA            complement(744..815)
                     /locus_tag="ACG912_002927"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(781..783),aa:Gly,seq:gcc)
     gene            complement(827..899)
                     /locus_tag="ACG912_002928"
     tRNA            complement(827..899)
                     /locus_tag="ACG912_002928"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(864..866),aa:Thr,seq:tgt)
     gene            complement(917..998)
                     /locus_tag="ACG912_002929"
     tRNA            complement(917..998)
                     /locus_tag="ACG912_002929"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(962..964),aa:Leu,seq:tag)
     gene            complement(1006..1078)
                     /locus_tag="ACG912_002930"
     tRNA            complement(1006..1078)
                     /locus_tag="ACG912_002930"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1043..1045),aa:Lys,seq:ttt)
     gene            complement(1082..1154)
                     /locus_tag="ACG912_002931"
     tRNA            complement(1082..1154)
                     /locus_tag="ACG912_002931"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1119..1121),aa:Val,seq:tac)
BASE COUNT          303 a          331 c          278 g          253 t
ORIGIN      
        1 ggagcaggat ttgaacctac gaccttcggg ttatgagccc gacgagctac cagactgctc
       61 catcccgcga taatatgaaa aaggaggata agggattcga acccttgcac ggttttaccc
      121 gcctgacggt tttcaagacc gttcccttca gccggacttg ggtaatcctc catatgaaac
      181 ttacaacgcc acaatggacc ttgtaggact cgaacctacg accggacggt tatgagccgt
      241 ctgctctaac caactgagct aaaggtccag gctcttaaaa atcgcggcgg aggggatcga
      301 acccccgacc tcccgggtat gaaccggacg ctctagccag ctgagctaca ccgcgaaaaa
      361 gatattcatt atttaaatga aatggagcct agcgggatcg aaccgctgac ctcctgcgtg
      421 caaagcaggc gctctcccag ctgagctaag gccccgtgtt taaatttata attatcggga
      481 agacaggatt cgaacctgcg accccttggt cccaaaccaa gtgctctacc aagctgagct
      541 acttcccgtt taattaacgc gcccaagagg agtcgaaccc ctaacctttt gatccgtagt
      601 caaacactct atccagttga gctatgggcg catatttgaa atgccgagga ccggaatcga
      661 accggtacgg tgatcactca ccgcaggatt ttaagtcctg tgcgtctgcc agttccgcca
      721 ccccggcgcg attgctttgg caaagcggaa aacggggttc gaacccgcga cccccacctt
      781 ggcaaggtgg tgctctacca ctgagctatt tccgcgtgtt taaagatgcc ggctaaagga
      841 cttgaaccct cgaccctctg attacaaatc agatgctcta ccaactgagc taagccggca
      901 tgtaaaactt aaattatgcg ggtgaaggga cttgaacccc cacgccgtaa ggcgctagat
      961 cctaaatcta gtgcgtctgc caattccgcc acacccgcaa aaatatgagc cgtacagggc
     1021 tcgaacctgt gaccctctga ttaaaagtca gatgctctac caactgagct aacggctcat
     1081 atggaggtta acgggatcga accgctgacc ctctgcttgt aaggcagatg ctctcccagc
     1141 tgagctaaac ctccatggat aactt
//
LOCUS       DAZKOW010000217         1088 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000217 DAZKOW010000000
VERSION     DAZKOW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.228"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            143..400
                     /locus_tag="ACG912_002932"
     CDS             143..400
                     /locus_tag="ACG912_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514168.1"
                     /translation="MSKLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="ACG912_002933"
     CDS             complement(632..1063)
                     /locus_tag="ACG912_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFQ0514169.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          414 a          142 c          139 g          393 t
ORIGIN      
        1 ttttctgtcc acgttcatat tactccaaag ttttcctcat tccaaaagaa aagcacagaa
       61 tacacaaaga agcgttttta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa attagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgacaaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaataa
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacatc ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaata
//
LOCUS       DAZKOW010000218         1087 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000218 DAZKOW010000000
VERSION     DAZKOW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.229"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..988
                     /locus_tag="ACG912_002934"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACG912_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          381 a          198 c          219 g          289 t
ORIGIN      
        1 atcttacgcc tgaacaaaaa gaattgttag aactacgtaa aagaaaccag caattagaaa
       61 tggaaaatga tattttaaag caagcagcgc tgatattcgg acgaagagac aagtaatcga
      121 tgcgaataag catctttacc ctatatcagc gatgtgcaga atattaggtc tatcacgtca
      181 gtcctattat tatcaatcaa aaccaaagaa agacgaatca gaacttgaag aagtagtcgc
      241 tgaagaattt atccgcagcc gaaaggccta cggctcaaga aaaataaaaa aagccttatc
      301 aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa atagaggatt
      361 aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca atgaagccaa
      421 aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag cgatcgtaac
      481 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
      541 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      601 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      661 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      721 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      781 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      841 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      901 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      961 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
     1021 catccgagga ggctcattgt caaggacaat cggaatagca tacggaaaga agttctgcac
     1081 cttataa
//
LOCUS       DAZKOW010000219         1018 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000219 DAZKOW010000000
VERSION     DAZKOW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.230"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(68..141)
                     /locus_tag="ACG912_002935"
     tRNA            complement(68..141)
                     /locus_tag="ACG912_002935"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(105..107),aa:Ile,seq:cat)
     gene            complement(696..1010)
                     /locus_tag="ACG912_002936"
     CDS             complement(696..1010)
                     /locus_tag="ACG912_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514170.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          377 a          160 c          157 g          324 t
ORIGIN      
        1 aaagctttag cttgtataat actcttcaaa aaattctaac acagaacgat tcaattggct
       61 acattaatgt accctgtagg actcgaacct acgaccggac ggttatgagc cgtctgctct
      121 gaccaactga gctaagggta gtggttgttg ccacataaag ccataaacaa tcaaccagta
      181 gaatgcgtgg caacaaacct gttatcgcat atcttggagt gtgactattt atgagtgata
      241 gtgaagatat gcgtctccat atatgtatgg ttgagacaga taaacatgta tgaaaaacgt
      301 tgttaaatca acgttttttt attttggtca aggagagaaa agtagagaaa tacaaaattt
      361 tttgcattga ttttgcattc aaaaaaacat tcaaacattt agaaatcccc aatacgtatt
      421 caggaaaatc tattttaagg tttacaaaaa gaacttttgt tcgtacactc tttttgaggt
      481 gaacttttat gctgatggaa ggaaaaacac aattatggtt taagcatgtt ccttcaaata
      541 agttttttaa ttataaactc tagagaaatt aattcatcct aacataaaat aattgttcac
      601 tcaaaagttt ttctagaaca attaaaacag caggcttcac attcgctata catttgtaag
      661 aagtaataat tcctgtacta aaaggcataa ttgctttaat tattttttga cttctcttca
      721 aacacaagaa gtagttgtgt aataattgca gaaataagga ttccaataat agatataaaa
      781 tttgacatgg aaacgtctat aacattaaaa gaataaagtg ttgaagaaag taaactgatt
      841 gaaataaccc ctccgtaaaa aatagtctgt gaatataccc actttttttc agaagacgta
      901 cttcgtttgg ttctaaaacc tatccctgca tttctactct tatcaaaaaa ccaaatattt
      961 agaagtaata atattgctat tattaagata aaattactgt aaaaatccaa aagctatc
//
LOCUS       DAZKOW010000220         1003 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000220 DAZKOW010000000
VERSION     DAZKOW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.231"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1003
                     /locus_tag="ACG912_002937"
                     /pseudo
     CDS             370..1003
                     /locus_tag="ACG912_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          184 g          295 t
ORIGIN      
        1 gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag aaagaaatga
       61 acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga aaaacaatct
      121 taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt tagcaagcta
      181 gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa atcattatgg
      241 atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa ttcgcaaaat
      301 ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc ccattgttat
      361 tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa gccgtcaaaa
      421 aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc aattgtggat
      481 aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat tttgcctaca
      541 cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga agcggtggaa
      601 tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt cttaccaaat
      661 gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc gttcaatgat
      721 tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga agccgagaaa
      781 gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca ctatgattac
      841 aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc aatgatgatt
      901 gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt ccatgagctg
      961 accgaagcct ttagagacaa ggaatcagac ttatttttta cct
//
LOCUS       DAZKOW010000221          974 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000221 DAZKOW010000000
VERSION     DAZKOW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.232"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..903
                     /locus_tag="ACG912_002938"
     CDS             <1..903
                     /locus_tag="ACG912_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFQ0514171.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
BASE COUNT          305 a          150 c          240 g          279 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgat
//
LOCUS       DAZKOW010000222          950 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000222 DAZKOW010000000
VERSION     DAZKOW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.234"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..>950
                     /gene="bsh"
                     /locus_tag="ACG912_002939"
     CDS             4..>950
                     /gene="bsh"
                     /locus_tag="ACG912_002939"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFQ0514172.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPII"
BASE COUNT          327 a          126 c          172 g          325 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga
//
LOCUS       DAZKOW010000223          946 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000223 DAZKOW010000000
VERSION     DAZKOW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.236"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(36..>946)
                     /locus_tag="ACG912_002940"
     CDS             complement(36..>946)
                     /locus_tag="ACG912_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514173.1"
                     /translation="KDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFS
                     DIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFEN
                     QFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYIN
                     DLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLS
                     LYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTI
                     QEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          306 a          161 c          145 g          334 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat ctttta
//
LOCUS       DAZKOW010000224          930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000224 DAZKOW010000000
VERSION     DAZKOW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.237"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..328)
                     /locus_tag="ACG912_002941"
     CDS             complement(<1..328)
                     /locus_tag="ACG912_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514174.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIK"
     gene            complement(335..883)
                     /locus_tag="ACG912_002942"
     CDS             complement(335..883)
                     /locus_tag="ACG912_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFQ0514175.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
BASE COUNT          314 a          188 c           92 g          336 t
ORIGIN      
        1 atttaatttt taaacaaaat tctccccaaa aaggaacttt ttctagtgaa aacttggtaa
       61 tctctttgta attaattctt gttaaagaat aattaaacac ttcattttta cttgttattt
      121 tttcattgac tataactagt tcatctggta aaaaaattaa ccaacaaaca tcgacaaaca
      181 gtaaatgata taatccattt ccacctccaa gcatattcaa tactggtgaa catgccttag
      241 aaacacgaat atagttatgg tttaaataga tattttcttt aaacattgct tgtttaaaag
      301 ccatatgctc ttgttctaaa cttttcataa acttttactc taatgcacag ctagcagcag
      361 ctgctgcaat tcctgctaca atatttacct tagctaactc tttagctgca gcttttccta
      421 attgtttttc tgcaattttt aataataaat ccgcagcttt ttcaaaatta aaagctttta
      481 atgcttcaat aaaatctccc tgcgtaccta tagcccaagc cactcctcct ccaggaagag
      541 aattaatggc tacacatcca gcatatcgtt tccaatcaac tttctccatt ctaggaacaa
      601 taatatgttc ataaaagact cttgctgctc ttccttgttc gttatcttga ctgattcgtt
      661 cacgaatagc accaaaatcc tttatatggt atacaccatg ttcgtctaaa aaaccaactg
      721 tatcaaaata aaaagttaat tcatctacta atttattctt tatttctaaa tcaactgtat
      781 tcactttttc tgtggcatac acacttgttc cagtactaac aaaagtactt aaacctaata
      841 agaaaacacc taaaacacca aatattttaa ttattctttt cataatttct cctccataaa
      901 aaataagtta ctttttaaat atcttgttta
//
LOCUS       DAZKOW010000225          910 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000225 DAZKOW010000000
VERSION     DAZKOW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.238"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG912_002943"
     CDS             <1..355
                     /locus_tag="ACG912_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514176.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="ACG912_002944"
                     /pseudo
     CDS             615..910
                     /locus_tag="ACG912_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          353 a          106 c          190 g          261 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa
//
LOCUS       DAZKOW010000226          899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000226 DAZKOW010000000
VERSION     DAZKOW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.239"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            256..879
                     /locus_tag="ACG912_002945"
     CDS             256..879
                     /locus_tag="ACG912_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0514177.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          270 t
ORIGIN      
        1 aataaactaa tgtccttatt tttgtgttca catttctgtt acctaagaaa tacttaaagg
       61 aggtgatgta gccagcaacg actacaactt gaacccgcta tctacagttt atttgttgtt
      121 tcttttgggt atactctaat ggtacaaatc catatttgct taaagcgttg atttttcagc
      181 ggtttttctt tattgcaaag tgtaccctaa gacctgtata ctaatagtaa atagattgga
      241 taggaatgat ggcaaatgta tttagcttat ttaagagttt cttccgacga tcaaagttta
      301 gctcggcaac ataagctaat tgaaaattgg tcagtcaaaa ataatattgc tgaagacgat
      361 ttaaaaattt tcgaggaaaa agtctctgga aaaaatattg aaaacagacc agagctgcaa
      421 gagcttattt ctttcatacg tgaacgagat acgattatta ttcctagtct tgatcgatta
      481 gcaagaaata gtaaagatac aaaagactta cttcaattgt ttcgtagtaa aggtgcttct
      541 attgaaatat tagacctacc gtcgtttaat ggggtaactg accctgcatt aagagacttg
      601 ctaacaaatt tagtcataga agtgtttagc tatgttgctg aaaacgaacg gaaaaaaatc
      661 aaagagcgtc aaaaacaagg cattgaaatt gcaaagaaaa atggtcgtta tactggtaaa
      721 aagattcaat accatgccaa tgcaacagga aaaaacaaat tgatttacga tgaaattgtt
      781 gatggtctga agaaaaaaga aactatttct aaaatagcaa agaacgcagg agttacaaga
      841 aaaaccgttt atcgagtaaa aagagattta ggaatgtaat atgttccagt tagaaagaa
//
LOCUS       DAZKOW010000227          899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000227 DAZKOW010000000
VERSION     DAZKOW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.240"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..878
                     /locus_tag="ACG912_002946"
     CDS             <1..878
                     /locus_tag="ACG912_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0514178.1"
                     /translation="SLNQPLPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENR
                     QLNPLRDYFLRYPRKVREQVRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRA
                     FTNHRIQVMKTFKKRDRRSKHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVD
                     RLLSYDDSLKRGYEVYQDFLSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRT
                     EIKRALRVPYSNGPIECLNNHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQ
                     LTKARTTTRLDKIAV"
BASE COUNT          288 a          201 c          183 g          227 t
ORIGIN      
        1 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
       61 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      121 ttgtggaaaa tcggcaacta aatcctttac gcgactattt cttgcgctac cctagaaaag
      181 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      241 aagagtgctt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      301 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa agggatcgtc
      361 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaaag
      421 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagca gagatagtcg
      481 atcggctatt atcttatgat gactccttaa agcgaggcta cgaagtctac caggattttc
      541 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
      601 aggagttgcc agagcactac caaccagtgt tcacgacctt caagaaatat cgaacagaaa
      661 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
      721 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac caagaacgaa
      781 tctttttata tcgaggaaaa tattttaaga aaactaagaa cactacccaa ctgactaaag
      841 ctagaacaac aacacggcta gacaagatag ctgtctagcc gtgtgagtat ctcgattat
//
LOCUS       DAZKOW010000228          890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000228 DAZKOW010000000
VERSION     DAZKOW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.241"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..888
                     /locus_tag="ACG912_002947"
     CDS             <1..888
                     /locus_tag="ACG912_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFQ0514179.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DAZKOW010000229          876 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000229 DAZKOW010000000
VERSION     DAZKOW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.242"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..434)
                     /locus_tag="ACG912_002948"
     CDS             complement(<1..434)
                     /locus_tag="ACG912_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514180.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG912_002949"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="ACG912_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFQ0514181.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          263 a          143 c          137 g          333 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgttat atcaactcat ttcttg
//
LOCUS       DAZKOW010000230          866 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000230 DAZKOW010000000
VERSION     DAZKOW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.243"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(202..600)
                     /locus_tag="ACG912_002950"
     CDS             complement(202..600)
                     /locus_tag="ACG912_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFQ0514182.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(597..833)
                     /locus_tag="ACG912_002951"
     CDS             complement(597..833)
                     /locus_tag="ACG912_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0514183.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          271 a          157 c          120 g          318 t
ORIGIN      
        1 ataaaaaatc agcccactag ttctaaacca ctatttccta ctattcactc ttttgtattc
       61 cttttaaatc aattatttca aaattaatcc atattttcca tatttttatg aaaaaactat
      121 tttgtatgat taaacataaa aaaatcagca tattccttaa tgacatgctg atctaattga
      181 gaaataaagt ttttaattcg ttcattttat tgcggtttgt cgtaaataac tggttatctt
      241 ggactttagt gaatcgaaat ccaatgaact agtggtaatc attatgataa aatcaacagc
      301 ttcttgacga gaaaaagcaa ctggacaatt attcaattga agaaacaaca acatggcaac
      361 aagagcagtt cgtttattgc cattatgaaa aggatgtttt ttagctaaat taattgctaa
      421 aatcgccgct ttttcataga tttcaggata gagttcttga ttgaatactg cttgtgaagg
      481 ctgtttgacg gccatatcta atgcatttgc atcttttaca ccgatcattt cgcctggcga
      541 gtgacgtaaa ataactttcg cgttgatttt gataatttct ttagtcgaaa gatacctcat
      601 aaatcaacca aatcttttaa cgcgtcttca tattgattca tgatcgaatc aactaatgaa
      661 tcaatatcag gttcttcttg tgctttgatc attcgtacag aaccatcaga ttgaaaaaca
      721 taactgattg cgtcacctgt ctgcacaccc atgaattctt ttacttctgg tgggatagtt
      781 gttacatcgc tattgccaga ttttctaatt ttatactttt tcattttttt catatttcgc
      841 caccctctat accattctca ttctat
//
LOCUS       DAZKOW010000231          820 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000231 DAZKOW010000000
VERSION     DAZKOW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.244"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(497..721)
                     /locus_tag="ACG912_002952"
     CDS             complement(497..721)
                     /locus_tag="ACG912_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514184.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT          296 a          112 c          146 g          266 t
ORIGIN      
        1 tacttctttt ttaatttgtt cttgcaatct tttttgctac gtaatttttg taaaaaagtg
       61 ggttgagaga aatctaattg attttcaaag aaggagtatg aaaatgagaa aaatatttat
      121 tcgtttattg tgggtactat cttagaggaa taaataatag tggagaaaac tatggtttgg
      181 cgcagattct atcaggtttg atggaacgaa gacaacctcc cgacatcaag tcaaaagcta
      241 cttgagattt tgatctgaaa ataaaaaaag aaaacaaggt aggaacatca atgtttctac
      301 cttgttttcg taatcaatgg gaaaaaacta agagtggtgt acgtaatcaa taaaaaggat
      361 ggtgacaaca gtacctcttt actctctgaa agcatcataa ctcgtttttc ctcattatgc
      421 caataaattg ttgttatacc acatataagg atttgtaata gctaaaaaca gacaggaaag
      481 ttgaaatctg tgaatcttat tttgtatgga aggatttata gaacgcaaaa atcaagataa
      541 agatgaataa aaatcctgtt tcggctatat attgcgtatc tacgccaata aaacgagttt
      601 gttctgtcat agccatataa agcaaaggca aaaatacaac tacagcgaga taagatccga
      661 tgatttctcg aaaagttttc caggaagtat gtttcctgtt tttatattta tccgatttca
      721 ttgaatagtc atagcctcct tattctttca tatgttgaaa ttccagtata aaaactggaa
      781 ggaactgtac gttttttaga ataaaaatta taagggataa
//
LOCUS       DAZKOW010000232          811 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000232 DAZKOW010000000
VERSION     DAZKOW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.245"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..672
                     /locus_tag="ACG912_002953"
     CDS             <1..672
                     /locus_tag="ACG912_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFQ0514185.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          298 a          126 c          152 g          235 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 ccacttcaag cgttaagaac cgtcaggttt a
//
LOCUS       DAZKOW010000233          800 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000233 DAZKOW010000000
VERSION     DAZKOW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.246"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..199
                     /locus_tag="ACG912_002954"
     CDS             <1..199
                     /locus_tag="ACG912_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514186.1"
                     /translation="KKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGI
                     IIAAIITQLFLVFGEKKRSKK"
     gene            complement(403..768)
                     /locus_tag="ACG912_002955"
     CDS             complement(403..768)
                     /locus_tag="ACG912_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514187.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          321 a           99 c          141 g          239 t
ORIGIN      
        1 taaaaaaaat tgggtatact cacaaactat tttttatgga ggaattgttt taattagttt
       61 actttcttca acactttatt ctttgaatat tatagatgtt tccacctcaa attcaatatc
      121 tattattgga atcattattg ctgcaattat tacacaactg tttttagtgt ttggagagaa
      181 gaaaagaagt aaaaaataga aaaactagtg atagttttgg tgatagtgat attaaaaatc
      241 agaaaagata gaaaaagcag gagatagata aaatgctgat atttcagcat tttttaagag
      301 tataaaagag aaaaaaagta ataaatgtat acatgatata agaaaaacat atatgtataa
      361 aaagaagaaa gtagcttctc tttgtacata tatggtttgt ttttatggat gccgagcttt
      421 tttataagat aaggctaccg cccataacga cccaaggatt tgtggaacta agtaaatacc
      481 tgcaagaagc aagatcagct gggtcggttt taaaggatga tagaagtaac gccattcagt
      541 aaaattggcg caaaataaat aggaagtgac agtagatata atattggcaa tgaataaaga
      601 tataatacgc ctgttccttt gaaaataata tccagcaaaa agagataaaa atatcaatgt
      661 gatgaacccg cttacggaat gataacgaaa atcaagcagt aaggcgaaat gcacataagg
      721 aacaaaagat aaaagcaacg gaaggttttt ccagcaagtt tttatcatct ttcatcacca
      781 cctaacaagt gttattattt
//
LOCUS       DAZKOW010000234          775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000234 DAZKOW010000000
VERSION     DAZKOW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.247"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            350..748
                     /gene="spxA"
                     /locus_tag="ACG912_002956"
     CDS             350..748
                     /gene="spxA"
                     /locus_tag="ACG912_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFQ0514188.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          289 a          108 c          137 g          241 t
ORIGIN      
        1 tttaaaatga cagaaaacat tactattaaa gaaataaaaa acataaaaaa tcttaagaaa
       61 tcggtttcat ttagcttttg agataaaata atagtacagt tttatacgct gtttcatatg
      121 tttagaaaat ttgaattaga tttgaaaact ctaattttta aatgataaaa aaagcgagaa
      181 tgctggtttt actgtatatt aaccttagat aactttcatt tttttaaaaa aattatgaaa
      241 aaaaggtagc tatttaaaag ttgattatgt ataatagaaa cagatgggag attattatga
      301 tcttctgtta caaaaaagtg attcttatca cgaaaaggag tggtgcacgt tgttaacatt
      361 atatacttcc ccaagttgta cttcctgcag aaaggctcgt gcgtggttgc aagaacatca
      421 gattccattt gttgaacgta atattttttc agaaccattg aacagttctg aattaaaagc
      481 gattttacaa atgacagaag acggaacaga agaaatcatc tcgacacgtt ccaaagtatt
      541 ccaaaaactg aacatggatt tagatgatct gccgttacaa gaattattgg aacttgtgca
      601 aaacaatcct ggattgcttc gccgtccaat catgattgat gataaacgtc tgcaagttgg
      661 ttttaatgaa gatgaaattc gtcggttcct accgcgcgat gtacgacaat tagaattacg
      721 tcaagcacaa ttgatggctg gattatagaa ggataatacg aaacttggaa aagac
//
LOCUS       DAZKOW010000235          773 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000235 DAZKOW010000000
VERSION     DAZKOW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.248"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(16..528)
                     /locus_tag="ACG912_002957"
     CDS             complement(16..528)
                     /locus_tag="ACG912_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514189.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            complement(535..>773)
                     /locus_tag="ACG912_002958"
     CDS             complement(535..>773)
                     /locus_tag="ACG912_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0514190.1"
                     /translation="DFNSSTEFKLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRG
                     TYIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
BASE COUNT          265 a          112 c           74 g          322 t
ORIGIN      
        1 ttaaaactaa catccttaat atcttaattt atccatattt tgagaaataa tacttttcca
       61 ttcagaaact aatttagtaa ggaatcttgc aatgttttgt ctcattaatt cattatttct
      121 attatctata agatctgtta gatatttttc aaattctaaa aagctatccg aattttcttc
      181 gacgattaaa tacttaagag aatttatatc ggaaaagata gattcaagtt tactaatctc
      241 attttctaaa tctatatttt cacgtgtgat tatctcatca ctttttctta aaaagctaaa
      301 aattgcatca gttgctttac acttttccat taaagaagta tattcttcaa tttttcttct
      361 ttctttatgg tcatttttta aatcagtttc aatgtctttt ttcatttgtt ctatactgag
      421 tttgttcttt ttgttctttt gagtaaatct aaaattcaaa cacgaaaaaa gtatgttagc
      481 aataacacct ataatagcaa ataaatcatt tatactattt aatttcattt atcatcacaa
      541 acttagaact ttacttatca tctcataaga caagtttcca aattgataat tattggtaga
      601 ttttaaatca ttataatttt cgagaaattt tatataagta cctctcatcg gtttatggct
      661 attaaaagtg ttaaaatcac tatcttcaac tatttcttga aacctattta atttttcaat
      721 aaaaaattgt gcataaagtg ctaacttaaa ttcggtagag ctattaaagt caa
//
LOCUS       DAZKOW010000236          771 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000236 DAZKOW010000000
VERSION     DAZKOW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.249"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..81
                     /locus_tag="ACG912_002959"
     tRNA            8..81
                     /locus_tag="ACG912_002959"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..210
                     /locus_tag="ACG912_002960"
     tRNA            121..210
                     /locus_tag="ACG912_002960"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            225..296
                     /locus_tag="ACG912_002961"
     tRNA            225..296
                     /locus_tag="ACG912_002961"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Glu,seq:ttc)
     gene            312..384
                     /locus_tag="ACG912_002962"
     tRNA            312..384
                     /locus_tag="ACG912_002962"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Val,seq:tac)
     gene            394..466
                     /locus_tag="ACG912_002963"
     tRNA            394..466
                     /locus_tag="ACG912_002963"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:427..429,aa:Asp,seq:gtc)
     gene            502..577
                     /locus_tag="ACG912_002964"
     tRNA            502..577
                     /locus_tag="ACG912_002964"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Phe,seq:gaa)
     gene            579..659
                     /locus_tag="ACG912_002965"
     tRNA            579..659
                     /locus_tag="ACG912_002965"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Tyr,seq:gta)
     gene            666..739
                     /locus_tag="ACG912_002966"
     tRNA            666..739
                     /locus_tag="ACG912_002966"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Trp,seq:cca)
BASE COUNT          173 a          174 c          213 g          211 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgccaaact
      181 gtctcaaggg ttcgaatccc ttactctccg tagcaaattt acctggcccg ttggtcaagc
      241 ggttaagaca ccgccctttc acggcggtaa cacgggttcg aatcccgtac gggtcatttt
      301 aattgaatgt tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
      361 gcggttcgat cccgttaacc tccataatga actggttccg tggtgtaggg gttaacatgc
      421 ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg gaaccgtcgt tcttgttatt
      481 caattaataa ttttaaaata tggcgcggta gctcagttgg tagagcaacg gattgaagct
      541 ccgtgtgtcg gcagttcgat tctgtcccgc gccaccatgg aggagtagcg aagtggctaa
      601 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
      661 tttatagggg catagtttaa aggtagaaca gcggtctcca aaaccgttag tgtgggttca
      721 attcctgctg cccctgccat ggcgaatgtg gcgaagtggt taacgcaccg g
//
LOCUS       DAZKOW010000237          770 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000237 DAZKOW010000000
VERSION     DAZKOW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.250"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            506..697
                     /locus_tag="ACG912_002967"
                     /pseudo
     CDS             506..697
                     /locus_tag="ACG912_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          299 a           92 c          125 g          254 t
ORIGIN      
        1 ggcagtggta tttcttcagt aagattaaaa gagaaagaat atactcaaaa gattattcat
       61 attcctaagc ctgaaattaa gcgttttgag ataaaagact attctgcttt agggctgttc
      121 tcaattggtt actggtgata aaaaataaga atactaaaac tatacgtaga atataagtaa
      181 tcggcatcaa tcctaattat gtgattgaat gccgattttt gttacgcgct atttttaatt
      241 atctgtaaga agaataattt tattttactc atattcgttt tcactttgaa gaagtaaatt
      301 gcaatttctt ataagttaag cagctatgca aaaggctatt tattctattg tgaaatataa
      361 gatgtagttg aaacaggata tatagaatca aaatacgaat cttattttga tttttctagt
      421 tttttgaaaa aagcaccctt tgatcgttgt attagtggaa aagagaaaaa ggagcatact
      481 tatcatgtca caaaactaca actaaatatc taaacaacac caatttgatg cctattgaaa
      541 aaaagtattg cgaaatgaag ccaagtctat tagaaaatga aataatagaa taaaagaaaa
      601 tgggaaaccg ctgaatgatg taaatgaagg taagtatcca cgaactaatt ttgatgaaca
      661 agatatctat tttttatttg tattggagat actagtttaa tatcaaaagt tatcagcagt
      721 cataattagt tatccaatgt aaaaagtaca aacaatagtt gttatgcaga
//
LOCUS       DAZKOW010000238          736 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000238 DAZKOW010000000
VERSION     DAZKOW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.251"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(91..>736)
                     /locus_tag="ACG912_002968"
     CDS             complement(91..>736)
                     /locus_tag="ACG912_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HFQ0514191.1"
                     /translation="MKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAI
                     VTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLF
                     HTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETL
                     AQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          201 a          171 c          140 g          224 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatg
//
LOCUS       DAZKOW010000239          719 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000239 DAZKOW010000000
VERSION     DAZKOW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.252"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..>719)
                     /locus_tag="ACG912_002969"
     CDS             complement(334..>719)
                     /locus_tag="ACG912_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HFQ0514192.1"
                     /translation="QLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLFI
                     LNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVES
                     NYKYSKKELLELLQKLNDHIEKIMP"
BASE COUNT          247 a          101 c          125 g          246 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgat
//
LOCUS       DAZKOW010000240          709 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000240 DAZKOW010000000
VERSION     DAZKOW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.253"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..600
                     /locus_tag="ACG912_002970"
     CDS             <1..600
                     /locus_tag="ACG912_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFQ0514193.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT          200 a          140 c          180 g          189 t
ORIGIN      
        1 accatgaaaa aagaatttcc gatcccgatt tcttggacaa cagatgtcaa tgcggctgcc
       61 tatggtgagt atgtcttcgg cagtggcaaa ggattgagca gtgtcgttta ctatacgatc
      121 ggtacgggtg ttggcggcgg tgccttacaa gaagggcgct ttatcgaagg gtttagtcat
      181 ccagaaatgg ggcatatgct cgtcgtccct catccaaaag atgactttgc tggcagctgt
      241 ccatttcatg gcaactgttt agaagggatg gccgctggac ccgccatcga aaaacgctta
      301 gggaaaaaag gacaagaggt tgcagaagat gatccttatt gggaaatcga agcctcctat
      361 attgctcaat gtgcctacaa tacaaccttg atgttttctc cagatgtgat catttttggc
      421 ggtggtgtga tgaaacaacg ccatatgctg caaaacgtcc acgacgcatt tgcaaaattg
      481 gtcaatgggt atgtcgaaac acctgagtta agcaaatata tcgtgacccc agcattagaa
      541 gacaacgctg gaacgctcgg ttgtttggca ttggctcgag aagcggtctt acattcataa
      601 acgaaaaaag gctcttcgac atttagtgtt ggtagagaaa gatacaactg aaaatttagt
      661 cataaccagt taggcagagt gcttaactgg tttttgtttt acttggaag
//
LOCUS       DAZKOW010000241          703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000241 DAZKOW010000000
VERSION     DAZKOW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.254"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>703
                     /locus_tag="ACG912_002971"
     CDS             <1..>703
                     /locus_tag="ACG912_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFQ0514194.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANY"
BASE COUNT          237 a          127 c          146 g          193 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ata
//
LOCUS       DAZKOW010000242          693 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000242 DAZKOW010000000
VERSION     DAZKOW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.255"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            132..344
                     /locus_tag="ACG912_002972"
     CDS             132..344
                     /locus_tag="ACG912_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514195.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>693
                     /locus_tag="ACG912_002973"
     CDS             506..>693
                     /locus_tag="ACG912_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFQ0514196.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          247 a          103 c          122 g          221 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct aga
//
LOCUS       DAZKOW010000243          690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000243 DAZKOW010000000
VERSION     DAZKOW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.256"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..461)
                     /locus_tag="ACG912_002974"
     CDS             complement(<1..461)
                     /locus_tag="ACG912_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFQ0514197.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          219 a          126 c           98 g          247 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac
//
LOCUS       DAZKOW010000244          670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000244 DAZKOW010000000
VERSION     DAZKOW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.257"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            154..417
                     /locus_tag="ACG912_002975"
     CDS             154..417
                     /locus_tag="ACG912_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFQ0514198.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..>670
                     /locus_tag="ACG912_002976"
     CDS             417..>670
                     /locus_tag="ACG912_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFQ0514199.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          253 a           88 c          140 g          189 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac
//
LOCUS       DAZKOW010000245          667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000245 DAZKOW010000000
VERSION     DAZKOW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.258"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>667
                     /locus_tag="ACG912_002977"
     CDS             <1..>667
                     /locus_tag="ACG912_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0514200.1"
                     /translation="LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYL
                     ERYFSRFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHR
                     VKETTVRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIAD
                     RLLAACPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLP
                     QIQ"
BASE COUNT          204 a          127 c          138 g          198 t
ORIGIN      
        1 ctttgccaaa ggtcttatgc tttgatgaat tcaaatcggt tcgaagtgtt gccagtgcaa
       61 tgagttttat catgatggac ggacaaagcc atgcattatt ggatattgtc gaaaatcgac
      121 gcttgcctta tttagaacgc tatttttctc ggttttcatt agcgactcgt gaagccgttc
      181 aattgattgt tattgatatg tatgcgcctt atgtttcgtt ggtgaaaaag cttttcccga
      241 atgctcagtt gatcatcgat cgtttccaca tcgtccaaca tattggcaga actttcttga
      301 atcatcgagt gaaagaaacc acggtacgac ttaaaggaaa ttcccactct caacgagggt
      361 tagggaaaaa attgaagcgc tattggaaac tcctacaaaa agaagaagca aagttaaact
      421 atgaaaaacg ggtttggcgt gccagtttta aagattattt gactgaaagt gagattgccg
      481 atcgactgtt agctgcttgt cccaacttac gccaaggcta tcagttatac caagacattc
      541 tttatgcggt aaagaaaaga gatcagtcac tatttgagga ttgtttaact agagacatta
      601 gcggtctgcc agaaacctat acaaccactt tacgaacgtt taaaaagttt ctgccacaga
      661 tccaaaa
//
LOCUS       DAZKOW010000246          656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000246 DAZKOW010000000
VERSION     DAZKOW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.259"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG912_002978"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="ACG912_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFQ0514201.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          198 a          143 c          111 g          204 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaa
//
LOCUS       DAZKOW010000247          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000247 DAZKOW010000000
VERSION     DAZKOW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.260"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..580
                     /locus_tag="ACG912_002979"
     CDS             <1..580
                     /locus_tag="ACG912_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFQ0514202.1"
                     /translation="GIKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDD
                     NPTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIA
                     NYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVY
                     VMMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          204 a          105 c          122 g          162 t
ORIGIN      
        1 aggaatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata cagatccttg
       61 cggttatctt tctgaaatgg gtgtaagtaa agaaaaacta gcctatgatt tggctcatgg
      121 ttttacggat gataatccaa ctacttcgga tgataatcca actacttcgg aggataaacc
      181 agtaattgat ccaactcgag caggtgctgc aaattctacg ctgacagatg gaacaaatta
      241 cgcccacgtt gatcagttcg gagaaatcga aaacgcaaac ttgcatgtag ctggatggca
      301 cattgctaat tataaatacg agtatatttt cattatggac tacaatactg gaaaagaact
      361 agctagagta agagctgatg ggatttatag accagacgta aatcaagctt ataatacttt
      421 aggaaatgtt ggctatcatg tatctttcaa tatgcgtgat ttccctaata agaaagtata
      481 cgtcatgatg cgtgcgacaa acgatccaga aggaaatact aagggtggag cacaagattt
      541 tcatgacaag cgttggtatt taaatattcc acaacgataa aaaattcccc cct
//
LOCUS       DAZKOW010000248          584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000248 DAZKOW010000000
VERSION     DAZKOW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.261"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(39..>584)
                     /locus_tag="ACG912_002980"
     CDS             complement(39..>584)
                     /locus_tag="ACG912_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFQ0514203.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          167 a          110 c          101 g          206 t
ORIGIN      
        1 gaaatccact ccttttttca tgctctaaaa tgatattttt acacaactgt tacggttgtt
       61 ttacgaaatt ggacgggact ttgccagttt aatttctgtt ttatccgttc attattgtag
      121 taatagatat agctatcgat ggctgatttt aattcctcga aacagttata aactttcccg
      181 tgaaaaatct cttgttttaa taagccaaaa aaattctcca taagtgaatt atctaagcag
      241 ttccctttcc gagacatgct ttgaaagatt ttgtactcct tcaatttgtt tctataagct
      301 ttcatttgat acgcccaacc ttgatctgag tgaaacgtac gacgataggg acaatcgtta
      361 gtggtctgga tagcttcctc caaaccttcc attaccgcta atgcattggg cttttcagag
      421 atacgatagg ataaaatctc actattgaac atatccataa aaggatctag atacaatttc
      481 ttttgacgga taatgccttt ggcatccact tcatggtatt taaattcagt tgtatccgtc
      541 gtgattttct gatggcagat actggtgtag aaacgacgat ggat
//
LOCUS       DAZKOW010000249          545 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000249 DAZKOW010000000
VERSION     DAZKOW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.262"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>545
                     /locus_tag="ACG912_002981"
     CDS             <1..>545
                     /locus_tag="ACG912_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFQ0514204.1"
                     /translation="HNCVPDKIKNRRNTDQLKQRDTVIIACVIWGIINGYTSQRATYR
                     AVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIKKLTKGELVGIIDSFPSPLCK
                     PVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITNPGVHDVKVLE
                     TLSEEANLPNILGDKGYISHK"
BASE COUNT          187 a          100 c           97 g          161 t
ORIGIN      
        1 ccacaattgt gtaccagata agattaaaaa ccgaagaaat actgatcaac tgaagcaacg
       61 cgatacagtg attattgctt gtgttatatg gggcataatt aatggctata ctagccaaag
      121 agccacgtat agagcggttt gttccgtctt atttcctaat ggtgactttc ctagtaggag
      181 tcgattcact cgcttaagtt caaacttagc ttacactatc aagattattc gatacttttt
      241 catcaagaag ctcaccaaag gtgaactagt cggaattata gatagttttc ctagtccatt
      301 atgtaaacca gttagaaata gacaagcaaa actattgaat caaatagcca aagttggcta
      361 taactcaaca aaaaaatctt atttttatgg tcttaaaatt catatgatcg ttactaagac
      421 tggctttccg attacttact caatcacgaa tccaggtgtc catgacgtga aagtgttaga
      481 gactttatcg gaagaagcga atcttcccaa tatcctaggg gacaaaggat atattagcca
      541 taaaa
//
LOCUS       DAZKOW010000250          527 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000250 DAZKOW010000000
VERSION     DAZKOW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.263"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(45..347)
                     /locus_tag="ACG912_002982"
     CDS             complement(45..347)
                     /locus_tag="ACG912_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFQ0514205.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          181 a           92 c           74 g          180 t
ORIGIN      
        1 ggtgataaaa tccgttccgg gctacctttt ttaggtagcc tactttaatc tttatacctt
       61 tctggatcaa cgaaagtata cttcaaatag tcataacgcc gatgatcgct ccgtgcgtct
      121 ggcacatcag tcacgatatc aaacaaaaaa tacacatctt tcttcattct ggtttttgtt
      181 gccggaattt taaagtagtt cttattagag taatagagat tgattaataa gctatcttcg
      241 attgctaaaa agaaaacttc tgaatcccac accttataaa aatctttgac aaatctattc
      301 gaaggatcaa atttaaacca taattgtgtt tttccttcca tcaacataat gttcacctca
      361 aaaagagtat acgaactaat gttctttttg taaagtagga atatcattga taatatggat
      421 ggaacatata tattaaaaag tgttcttttt gaatgctatt ttgaatgcca ttttcattca
      481 tttaacgaaa atctgcgaaa ataaaaaatg ctcaaaccct tataaaa
//
LOCUS       DAZKOW010000251          526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000251 DAZKOW010000000
VERSION     DAZKOW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.264"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..354
                     /locus_tag="ACG912_002983"
     CDS             <1..354
                     /locus_tag="ACG912_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFQ0514206.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
BASE COUNT          209 a           75 c          115 g          127 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacaga
//
LOCUS       DAZKOW010000252          524 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000252 DAZKOW010000000
VERSION     DAZKOW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.265"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(135..>524)
                     /locus_tag="ACG912_002984"
     CDS             complement(135..>524)
                     /locus_tag="ACG912_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0514207.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          157 a           79 c           89 g          199 t
ORIGIN      
        1 gaaaaaatta actagcacca cgggttatag ttttattttt atatccatgt atgcaaccta
       61 aacatctaat tataaaaatt atgggagcgt caataatttt gtgtaaataa attgtcctcc
      121 tgcaaaataa ttagttactc agtaaacatt gaaactaatg tatcggttac ctgttgaaaa
      181 cctttatggc ttctgtttag aaatttttga ttgtatgtat caaaaatgct gactagaaag
      241 cgttctagtg attcttcatt ttgaaactgc tcttttctac ggctgtatct tttaatttgc
      301 ttattgaaag actcgattag attggttgag taaatggttc tacgaatgct aggtggaaaa
      361 tcataaaaag ttaataagtc ttggttttct atgagtgact gcgtcacttt aggatagttt
      421 ttcttccatt tctcaatcat gccggataag aaggtattcg cttcttcttt tgagttagct
      481 tgataaacag ccttaaagtc atcacagatt tcttttcggt cttt
//
LOCUS       DAZKOW010000253          512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000253 DAZKOW010000000
VERSION     DAZKOW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.266"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..343
                     /locus_tag="ACG912_002985"
     CDS             <1..343
                     /locus_tag="ACG912_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514208.1"
                     /translation="DNIRDKKASLKSLKDTWLDLSEDNPYSQFLITVMAGVNQLERDL
                     IRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKLYKEGNMTVNQICEITNVSRA
                     SLYRKLSEVNN"
BASE COUNT          206 a           69 c           93 g          144 t
ORIGIN      
        1 cgataacata cgagataaaa aggcaagttt aaaatcacta aaagatacat ggcttgattt
       61 atcagaagat aatccataca gccaattctt aattactgta atggctggtg ttaaccaatt
      121 agagcgagat cttattcgga tgagacaacg tgaagggatt gaattggcta agaaagaagg
      181 aaagtttaaa ggtcgattaa agaagtatca taaaaatcac gcaggaatga attatgcggt
      241 aaagctatat aaagaaggaa atatgactgt aaatcaaatt tgtgaaatta ctaatgtatc
      301 tagggcttca ttatacagga aattatcaga agtgaataat tagccattct gtattccgct
      361 aatgggcaat atttttaaag aagaaaagga aactataaaa tattaacagc ctcctagcga
      421 tgccgaaaag ccctttgata aaaaaagaat catcatctta agaaattctt agtcatttat
      481 tatgtaaatg cttataaatt cggccctata at
//
LOCUS       DAZKOW010000254          500 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000254 DAZKOW010000000
VERSION     DAZKOW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.267"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          168 a           96 c           58 g          178 t
ORIGIN      
        1 aaataagaat gctaaatcac tctacaataa cggcgaaagc acaaaaaata aaaaaattat
       61 tacgttatta aaaaaacaga tgaaagatca ggttttatta gatctcattc tttcatcact
      121 tgaagggatt taatgaacta ctttttcctc tttgtcgttt ttatatattc gtattccttc
      181 aaacagtagg atagcctctt tcaatgcttg atatttgaac tgtccgttac ttaaaaattt
      241 agatgtgccg ccctcttcct agaacatatt cttttttact ttttcattaa ttgacagacc
      301 aatcgaacga aactgtaaat ttcgatagga taatgattgg tcaaactcat tcattttcaa
      361 gacatctcga ttcatattca agataggaat ctttttatca acatcacttt attcgtattt
      421 tcctctacca taaaagcttc acgctccaca aaattgtttt atacactact attcagccca
      481 ttcaacgacc aatgaatttt
//
LOCUS       DAZKOW010000255          495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000255 DAZKOW010000000
VERSION     DAZKOW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.268"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..165
                     /locus_tag="ACG912_002986"
     CDS             43..165
                     /locus_tag="ACG912_002986"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514209.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
BASE COUNT          185 a           73 c           87 g          150 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc catcgccgat tgtaaaatta
      361 agctagacaa ctaaaaaagt catttgtgct acactcaaaa tagttccgac caaagaatta
      421 taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg taataataga
      481 gtcttatttc aaaat
//
LOCUS       DAZKOW010000256          476 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000256 DAZKOW010000000
VERSION     DAZKOW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.269"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            22..>476
                     /locus_tag="ACG912_002987"
     CDS             22..>476
                     /locus_tag="ACG912_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0514210.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNISERKK"
BASE COUNT          178 a           87 c           95 g          116 t
ORIGIN      
        1 atacaaggag agtgaacata aatgacttat acccatctta caccaaacga gcttgtaatg
       61 atagaagcat attttcatca agaaactccg gttgctatcg ttgcgaagca gcttaaacgt
      121 ggacgccaaa caatttacaa tgtctataac tttctcaaat gtggtggaac agcacttgaa
      181 tactttgaac aatacaaaga aaataagcga cgttgtggga gaaccgaaat catttttcct
      241 gctgaggaaa aagaatacat tgaaaaaaga tcaactgaag gttggacccc agacgtcatt
      301 attggccgtg cagagcgaac cttctcttgt tcagtaagta ccctctatcg ccggtttaag
      361 acgggagaat tcaatgtttt acatttaccg atgcaaggaa aacgaaaacc aaatggttat
      421 aaggaaaaac gtggaaaaca ggcattcaaa agaaatattt ctgaacgtaa aaaaga
//
LOCUS       DAZKOW010000257          460 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000257 DAZKOW010000000
VERSION     DAZKOW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.270"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACG912_002988"
     tRNA            complement(20..91)
                     /locus_tag="ACG912_002988"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACG912_002989"
     tRNA            complement(113..200)
                     /locus_tag="ACG912_002989"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACG912_002990"
     tRNA            complement(225..298)
                     /locus_tag="ACG912_002990"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACG912_002991"
     tRNA            complement(312..382)
                     /locus_tag="ACG912_002991"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACG912_002992"
     tRNA            complement(384..459)
                     /locus_tag="ACG912_002992"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAZKOW010000258          439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000258 DAZKOW010000000
VERSION     DAZKOW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.271"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..422
                     /locus_tag="ACG912_002993"
     CDS             93..422
                     /locus_tag="ACG912_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFQ0514211.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          169 a           52 c           79 g          139 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaac
//
LOCUS       DAZKOW010000259          421 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000259 DAZKOW010000000
VERSION     DAZKOW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.272"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(170..355)
                     /locus_tag="ACG912_002994"
                     /pseudo
     CDS             complement(170..355)
                     /locus_tag="ACG912_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
BASE COUNT          120 a           85 c           62 g          154 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagaggtt
      361 ctgttgcgaa gtttgaaaat caaacgcgcc ctctctgaac tccaaatatc ttaggctgtt
      421 a
//
LOCUS       DAZKOW010000260          411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000260 DAZKOW010000000
VERSION     DAZKOW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.273"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>411
                     /locus_tag="ACG912_002995"
     rRNA            <1..>411
                     /locus_tag="ACG912_002995"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAZKOW010000261          410 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000261 DAZKOW010000000
VERSION     DAZKOW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.274"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          152 a           49 c           51 g          158 t
ORIGIN      
        1 ttacgcttta aactctttta tatttgaatt cactaagaag cataccatat ttttgaaaaa
       61 aagtgagaaa aaaggcttat aattaggatt tagagtaatt aattagatgt ttattttata
      121 ggactgatat tatagattac aaatagtatc gtatttcgca atcttaaaat tatcttctta
      181 ctatgaataa gataataaaa tttgtatttg tcctttttag gtaacttatt ttaatctata
      241 tacctttctg gatcaacgaa agtatacttt atatagtcat aacgccgatg gtaactatgc
      301 actgtaacga taatgctgct aaaatatctt tttacgaaaa acatcacaag gaaaaactta
      361 actatggtat tatctaaaca taattttttc ggtatttaat atttaattag
//
LOCUS       DAZKOW010000262          392 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000262 DAZKOW010000000
VERSION     DAZKOW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.275"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            185..>392
                     /locus_tag="ACG912_002996"
     CDS             185..>392
                     /locus_tag="ACG912_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514212.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
BASE COUNT          127 a           38 c           67 g          160 t
ORIGIN      
        1 tgatgctctt acattcggta aaactacttt ttctagtttt aattttcagt ttctattaaa
       61 aaataatttt tatggtttat tgaaataagg ttgcgaagga agatgtagag ttatttgcac
      121 tgttgtgttg gtatatctct taatgctgtg tctttttggg gagattaatt agtggagtga
      181 ttatatgagt atagatgatt gtttagaaga acaaataaga ttatttttaa agtttttaaa
      241 agttaaagtt caaaataact acttcacatg ttatagttat gcacaccctg ttttaaatag
      301 tcttcctttt aattctttag tgcaaggatt gattacaaat attgttttat tagtctgtac
      361 accaaatgaa atatttgtaa aaaagatagg ca
//
LOCUS       DAZKOW010000263          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000263 DAZKOW010000000
VERSION     DAZKOW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.276"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG912_002997"
     CDS             <1..>386
                     /locus_tag="ACG912_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0514213.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLIKQSKRLFPEINCVVADA
                     EYKTPKFVHFLTHLNLRPCLPYSRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTRERYREYKSNPKECVNCPLLNQCT"
BASE COUNT          120 a           80 c           67 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctca tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgagtata agacacctaa gtttgttcac tttttgactc atttaaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gggctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaaa ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKOW010000264          386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000264 DAZKOW010000000
VERSION     DAZKOW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.277"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>386
                     /locus_tag="ACG912_002998"
     CDS             <1..>386
                     /locus_tag="ACG912_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0514214.1"
                     /translation="CDDNGFILSTIITPRNIHDSQVAFQLVKQSKRLFPEINCVVADA
                     GYKTPKFVHFLTHLNLRPCLPYSRPKGKKELLSKNEFLYDEYFDCYICPQDQMLAFST
                     VTREGYREYKSNPKECVNCPLLNQCT"
BASE COUNT          117 a           80 c           70 g          119 t
ORIGIN      
        1 atgcgacgac aatggattta ttcttagcac aatcatcact cctagaaata ttcatgacag
       61 tcaagtcgcc tttcagctcg tcaaacaatc gaagagactt tttcctgaaa tcaattgtgt
      121 tgtcgctgat gctgggtata agacacctaa gtttgttcac tttttgactc atttgaacct
      181 tcgaccttgt ttgccttatt caaggcccaa agggaaaaag gagctattat caaaaaatga
      241 gtttctctat gacgagtatt ttgattgtta tatttgtccg caagatcaaa tgctcgcttt
      301 ttcaacggtt actcgagaag ggtaccgaga atacaaatcc aatccaaaag aatgtgtgaa
      361 ctgtcctctg ctaaaccaat gtacca
//
LOCUS       DAZKOW010000265          382 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000265 DAZKOW010000000
VERSION     DAZKOW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.278"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..277
                     /locus_tag="ACG912_002999"
     CDS             <1..277
                     /locus_tag="ACG912_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFQ0514215.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
BASE COUNT          132 a           66 c           79 g          105 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tg
//
LOCUS       DAZKOW010000266          376 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000266 DAZKOW010000000
VERSION     DAZKOW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.279"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..376
                     /locus_tag="ACG912_003000"
                     /pseudo
     CDS             166..376
                     /locus_tag="ACG912_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT          142 a           48 c           71 g          115 t
ORIGIN      
        1 taatcttctt tatagagaat tgggctattt ttaatggcaa agaggggaag aagatgtttc
       61 aaaacaaagg acaacgctat gttgtaaaag gagtaaagga atcttactgg aatcaaaaat
      121 ctttattgag aaaattatct ttgaatttaa gcacaaagaa atgaaggtac actagatagc
      181 aatgaagata tctttgtcat taaacgtcgc tattttacac aagtaagtga tgatttgatt
      241 catgtctttt ccaatgagtg gtttgtttct gagaaaaaat tacatgcttc taatctgcaa
      301 tatatgccag gtgaagatcc aattcctaat atgaaagcaa ttatcaacag taaggattac
      361 gaaggatata aagcca
//
LOCUS       DAZKOW010000267          370 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000267 DAZKOW010000000
VERSION     DAZKOW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.280"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>370
                     /locus_tag="ACG912_003001"
     CDS             82..>370
                     /locus_tag="ACG912_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFQ0514216.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCR"
BASE COUNT          132 a           59 c           76 g          103 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa
//
LOCUS       DAZKOW010000268          362 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000268 DAZKOW010000000
VERSION     DAZKOW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.281"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>362)
                     /locus_tag="ACG912_003002"
     rRNA            complement(<1..>362)
                     /locus_tag="ACG912_003002"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAZKOW010000269          358 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000269 DAZKOW010000000
VERSION     DAZKOW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.282"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>358
                     /locus_tag="ACG912_003003"
     CDS             <1..>358
                     /locus_tag="ACG912_003003"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFQ0514217.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDY"
BASE COUNT          160 a           51 c           52 g           95 t
ORIGIN      
        1 aatgtggtta gaaaaaataa atgacaaaaa atttaagtac accgaacgtt atacggatcc
       61 cttaactgaa aaaaagagaa aagtgtccgt tactttatct agcaattcac gccaagcttg
      121 gaatcaagct aacttacttt tgaatgaaaa aatcgcagaa aaaataaaac gaaatgaaga
      181 attaccacta acttttggag aattaaaaac taaatgggat gaaaaataca agccaactgt
      241 aaaagaaagt tcctatcgaa caactcaggt ttatctatca ttaatttcaa aatatataaa
      301 agatgatgta cttgtaaaaa acgttaatag taatcttata caagatatgc tagattac
//
LOCUS       DAZKOW010000270          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000270 DAZKOW010000000
VERSION     DAZKOW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.283"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            73..>321
                     /locus_tag="ACG912_003004"
     CDS             73..>321
                     /locus_tag="ACG912_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0514218.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLE"
BASE COUNT          106 a           59 c           60 g           96 t
ORIGIN      
        1 aaaaaaatat agaaaagaca ccctgaaatc ctttagaatt aatttggcta ccaattcata
       61 ggaggattca agatgtctca tactcagctt atcaaagata ccctaaatat tctagaccta
      121 aatatctatt tcgaggaaaa ttgtttaact aaagaaaaat acaaagggca aatttgcttg
      181 atttacaaag gaactcttct gtacaagcca gaagagtgtt cccattgtct ttgtgtcgtc
      241 ccctctagga tcattcgttg ggggacgaca accgttcggt tgttactgaa tgatgtgtct
      301 gagtatcgca cttatctgga g
//
LOCUS       DAZKOW010000271          321 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000271 DAZKOW010000000
VERSION     DAZKOW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.284"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>321
                     /locus_tag="ACG912_003005"
     CDS             <1..>321
                     /locus_tag="ACG912_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HFQ0514219.1"
                     /translation="TQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTK
                     TEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFG
                     FSVST"
BASE COUNT          107 a           69 c           63 g           82 t
ORIGIN      
        1 acgcaagcag cctatgcttt cttaaaacga ctccataaac agtttggtga gccgaaagca
       61 attgtgaccg ataaagcacc ttctcttggc tccgccttta gaaagttaca gagtgtgggt
      121 ttatatacta agacagagca ccgaactgtg aagtatctta acaatttaat agaacaagac
      181 catcgaccta ttaaacgacg aaataaattt tatcaaagtc tccgtacagc ctcttccacg
      241 attaagggca tggagaccat tcgaggaata tataaaaaga accgaagaaa tggaacgctc
      301 ttcggctttt cggtgtctac t
//
LOCUS       DAZKOW010000272          318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000272 DAZKOW010000000
VERSION     DAZKOW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.285"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(86..>318)
                     /locus_tag="ACG912_003006"
     CDS             complement(86..>318)
                     /locus_tag="ACG912_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0514220.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT          101 a           47 c           57 g          113 t
ORIGIN      
        1 tacaaaaaag atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac
       61 tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa
      121 tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga
      181 tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa
      241 taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt
      301 caggaagatt ttttaata
//
LOCUS       DAZKOW010000273          315 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000273 DAZKOW010000000
VERSION     DAZKOW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.286"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>315
                     /locus_tag="ACG912_003007"
     CDS             <1..>315
                     /locus_tag="ACG912_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514221.1"
                     /translation="HWNAIEGKPTTVKGDKGDPGQAATITIGTVSSGSTASVTNVGTS
                     SAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDKTKLDGIAAGAQKNPGNAT
                     TTT"
BASE COUNT          109 a           62 c           72 g           72 t
ORIGIN      
        1 tcattggaat gctatagaag gcaaaccaac aacggtgaag ggagataaag gcgatcctgg
       61 ccaagcagca acaattacca taggaaccgt ttctagtggt tcaaccgctt ctgttacgaa
      121 tgtaggaaca tcatctgcag ctagatttaa ttttgtttta ccaaagggag ataaaggtga
      181 tcctggaata aatgccacaa ctacagctgt agctacaact acagcaaatg ggttgatgtc
      241 gtccacagat aaaactaagt tggatggaat tgcagctgga gcacagaaaa atccaggtaa
      301 tgctacaact acgac
//
LOCUS       DAZKOW010000274          291 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000274 DAZKOW010000000
VERSION     DAZKOW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.287"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>291
                     /locus_tag="ACG912_003008"
     CDS             <1..>291
                     /locus_tag="ACG912_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFQ0514222.1"
                     /translation="QQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQE
                     YGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLR"
BASE COUNT           98 a           43 c           58 g           92 t
ORIGIN      
        1 tcagcaggat gtgattattg tagccgtggg ctactatctt cgttataacc ttagctatcg
       61 tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt tctcatacga cgatttatcg
      121 ttgggtgcaa gaatatggca aactactcta tcaaatttgg aaaaagaaaa ataaaaaatc
      181 cttttattca tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggcattattt
      241 gtatcgagcc atcgatgcag atggtttaac cttggatatt tggttacgta a
//
LOCUS       DAZKOW010000275          285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000275 DAZKOW010000000
VERSION     DAZKOW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.288"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..285
                     /locus_tag="ACG912_003009"
                     /pseudo
     CDS             1..285
                     /locus_tag="ACG912_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          110 a           58 c           56 g           61 t
ORIGIN      
        1 acaacaaatc gtcgatctct atctcgctgg taagcctcgc gcagaaatta ttcgagagta
       61 tgagcttacg ccttcttctt tcgataaatg gatgaagcaa gcacaatcaa cgggctcatt
      121 caaagaaaga gacaacttaa caccagaaca agcagaattg atcgcactaa gaaagaaaaa
      181 taagcaactc gaaatggaga atgatatttt aaagcaagcg gcgctgatat tcggacgaaa
      241 agacaagtaa ttgatgccaa caagcataaa tattccatat cagcg
//
LOCUS       DAZKOW010000276          244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000276 DAZKOW010000000
VERSION     DAZKOW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.289"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..227
                     /locus_tag="ACG912_003010"
     CDS             <1..227
                     /locus_tag="ACG912_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008376682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFQ0514223.1"
                     /translation="PLKEKFLNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYR
                     VAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT           86 a           26 c           52 g           80 t
ORIGIN      
        1 agccattgaa agaaaaattt ttgaatttaa tttatgatga aattgcgatt gatccatata
       61 agggtgaaca aaaaactggg gatttatcag gtatttggtc tagtgggttc aaatatgcgg
      121 gcacaacata tagagtagct tacgagatta tagaaaacaa ggttattcca gtattgttat
      181 gtggaacgca tgaaaacttt tatgaacagt tgaaaaaatt aatctagtga tttgttgtcg
      241 ttct
//
LOCUS       DAZKOW010000277          232 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000277 DAZKOW010000000
VERSION     DAZKOW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.290"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>232)
                     /locus_tag="ACG912_003011"
     CDS             complement(<1..>232)
                     /locus_tag="ACG912_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514224.1"
                     /translation="LLNDVSNYKTILRINKQRFKCKHCGKTFLAEDSVSDRRCSIARR
                     VKQAILELLSEPLSMSLIARMKHISPTTVIRIL"
BASE COUNT           70 a           42 c           53 g           67 t
ORIGIN      
        1 aagtattcga atgacagtgg tcggagaaat gtgcttcata cgtgcgatca aagacataga
       61 aagaggttcg cttaatagct caagaatggc ttgtttgact ctgcgagcaa tggagcagcg
      121 acgatcacta actgaatctt ctgctaaaaa agtctttcca cagtgtttac atttgaagcg
      181 ctgtttgtta atccgcagaa tggtcttgta gttactgaca tcattcaata ag
//
LOCUS       DAZKOW010000278          230 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000278 DAZKOW010000000
VERSION     DAZKOW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.291"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           79 a           35 c           45 g           71 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac
//
LOCUS       DAZKOW010000279          224 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000279 DAZKOW010000000
VERSION     DAZKOW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.292"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>224
                     /locus_tag="ACG912_003012"
     CDS             <1..>224
                     /locus_tag="ACG912_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFQ0514225.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAZKOW010000280          222 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000280 DAZKOW010000000
VERSION     DAZKOW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.293"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>222
                     /locus_tag="ACG912_003013"
     CDS             <1..>222
                     /locus_tag="ACG912_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFQ0514226.1"
                     /translation="SFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRS
                     HASIEDKMSFICADGETGQLIDVLPTRKL"
BASE COUNT           67 a           40 c           45 g           70 t
ORIGIN      
        1 tatcttttat tgcgaaaaat tgtcaggtat ctttgaccac cgttattcgt acgttgaaag
       61 agtttaaaag ctacttacca aagcaatcta agaggattct ccccagagta ttgatggttg
      121 atgaatttcg ttcgcacgct tccatagaag ataaaatgag ctttatttgc gcagatggcg
      181 aaacaggaca attaattgat gtcttgccta cgcgtaaatt gc
//
LOCUS       DAZKOW010000281          220 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000281 DAZKOW010000000
VERSION     DAZKOW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.294"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..175)
                     /locus_tag="ACG912_003014"
     rRNA            complement(<1..175)
                     /locus_tag="ACG912_003014"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           48 a           60 c           49 g           63 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc
//
LOCUS       DAZKOW010000282          206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000282 DAZKOW010000000
VERSION     DAZKOW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.295"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>206)
                     /locus_tag="ACG912_003015"
     CDS             complement(<1..>206)
                     /locus_tag="ACG912_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFQ0514227.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DAZKOW010000283          206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000283 DAZKOW010000000
VERSION     DAZKOW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.296"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>206
                     /locus_tag="ACG912_003016"
     CDS             <1..>206
                     /locus_tag="ACG912_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFQ0514228.1"
                     /translation="VKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNN
                     LIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT           74 a           44 c           37 g           51 t
ORIGIN      
        1 gtgaagcagt ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt
       61 gcctttaaga aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa
      121 tacctgaata atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat
      181 cgaagtttac gcactgcctc taccac
//
LOCUS       DAZKOW010000284          206 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000284 DAZKOW010000000
VERSION     DAZKOW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.denovo.297"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT           93 a           30 c           29 g           54 t
ORIGIN      
        1 tcacagcatg cttaaaaggt ctatggatta agttatgtaa aaaagtgcct ctttaaacgt
       61 cctaaaatcc aaacaaggat agcataatat acactatcgt tgatccagaa aacaggtaaa
      121 taaattttgc aaaagcaata agttaaaaac attacaaaaa agacaaggat atcacatcaa
      181 gaaaaaatgt tttgaaaata tctttg
//
LOCUS       DAZKOW010000285         8373 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.116, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000285 DAZKOW010000000
VERSION     DAZKOW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.116"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..729)
                     /locus_tag="ACG912_003017"
     CDS             complement(<1..729)
                     /locus_tag="ACG912_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFQ0514229.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGI"
     gene            857..1426
                     /locus_tag="ACG912_003018"
     CDS             857..1426
                     /locus_tag="ACG912_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFQ0514230.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            1493..2230
                     /locus_tag="ACG912_003019"
     CDS             1493..2230
                     /locus_tag="ACG912_003019"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFQ0514231.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            2461..3729
                     /locus_tag="ACG912_003020"
     CDS             2461..3729
                     /locus_tag="ACG912_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFQ0514232.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4216..4680
                     /locus_tag="ACG912_003021"
     CDS             4216..4680
                     /locus_tag="ACG912_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFQ0514233.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            4664..5293
                     /locus_tag="ACG912_003022"
     CDS             4664..5293
                     /locus_tag="ACG912_003022"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFQ0514234.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            5647..7125
                     /gene="msr(C)"
                     /locus_tag="ACG912_003023"
     CDS             5647..7125
                     /gene="msr(C)"
                     /locus_tag="ACG912_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFQ0514235.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7172..7618
                     /locus_tag="ACG912_003024"
     CDS             7172..7618
                     /locus_tag="ACG912_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFQ0514236.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(7817..8332)
                     /locus_tag="ACG912_003025"
     CDS             complement(7817..8332)
                     /locus_tag="ACG912_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFQ0514237.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
BASE COUNT         2742 a         1496 c         1701 g         2434 t
ORIGIN      
        1 gatcccatct agagctaatg caccaaaagt cccgttatcg accatcaaag aagcattgta
       61 caataagtta tatgttccga cgatcgataa ctgatctatg ctgaaaccta gccattcagc
      121 tagttgactg tccactgacg tttgccggga aatgatcaaa ggaacagttt tcagttcctg
      181 cggatggatg actgtttgct ttgctaaagg atgattccga tgtatcaaaa gtccccaacg
      241 atcttgggct ggcagtcgaa atgactggta tttttgtttg tttgtcggtt cgattacgag
      301 tccaaaatca agtaatccgc tatccagttt gtctatgata tcatctgcat tcccgctata
      361 taaatgaaac tggatatcag gatgcgcctt gatcaacgaa tgaaggctat cagaaatgaa
      421 agagattgct ttagtttcgc ctccgccaat gtagatttct ccagagatga ttgcgtcatt
      481 tgttaaattg ttcgccgttt tttctaccaa cgacaagatt tcttttcctt ttgaataaag
      541 atatcgacca gaatccgtca actgtatctc tcgggagcct cgagtgaaaa gtgtcacacc
      601 aagttcttct tctaaatctt tcagttgttt tgagagagta ggttgcgtga tatgcagaga
      661 ttttgctgct cggctgatat ttttttctct agctactgct aaaaaatacg tcagtatacg
      721 aatatccatt ttttccctcc tgtttttaaa aaagcatacc actaagtata gttaaaagga
      781 atagttaagt atgttttata agtattagac atcataagcc gtccgttata aagtaaggat
      841 aaaaggaggg agcaaaatga caagcctcat cgaaaaacaa atcgtcaata aagagattcg
      901 agtaacggat ccgctctttg aagtgatcca tcagatccaa gcggaaaacg aagagccttt
      961 agcagcgctg aatactgggt accatgaacc agcagatatc cgcaaacggc tagaaaatat
     1021 cattagtgac aaagtagatg atacggtgac tgttctgctg cctttttata cagatttcgg
     1081 caaacatatt agtataggga aaaatgtctt tatcaatcgt caagtgatgt tcgttgactt
     1141 aggtggcatc tgtttagaag attcagtatt gattggtcct cgtgtcaacc tcatcacggt
     1201 gaaccatcta accgatccaa aagaacgaag aggcttgtca gtaaagccaa tccacatcaa
     1261 gaaaaatgct tggatcggag caggagcaac tattctgcca ggcgtaacga ttggcgagaa
     1321 tgccattgtt gcagcgaatg caactgttac gaaagatgtc ccagacaata ccatcgttgc
     1381 aggaatccct gccaaaatcg tcaaacctgt cgaacggatt atttgaacag taagatgaaa
     1441 taagagatag tgagtataag caagagattt ttcaaaacag gaggagaata gaatgaatga
     1501 tttatcagaa aaagtgattg tcatcatggg cgcttcaagc ggaatcggtg aagcaacaac
     1561 gaagctatta gcagaaaaag gggctaaact agttatagct gcacgccgag aagatcgcct
     1621 aaaagcaata aaagaatctt taccagaagc tgagctatat attcaaacag cagatgtaag
     1681 agattttgca caagtccaag cagttatcga tttagcgatg gaaaagttcg gtcgaatcga
     1741 tgtattgtac aataatgcag gaatcatgcc aaccgctcca ttagttgaag gtcaccgtga
     1801 tgaatggcaa aatatgcttg atatcaacat catgggtgtg ttaaatggaa tttccgccgt
     1861 acttccaatc atggaaaaac aaaaatctgg gcatatcatt tcaactgatt ctgtggcggg
     1921 acatgttgtt tatcctgatt ccgccgttta ttgcggaaca aaatttgctg tccgtgcaat
     1981 catggaagga ttacgtcaag agcaacggca aaataacatc aaatctacga tcatctctcc
     2041 aggagctgtt caaactgaat tatatcaaac catcagcgac aaaaaggctg ctcttgaact
     2101 tcatgaagcg caaaaagaat ggggattaac atctgaagat atagcttctg ctgtagcatt
     2161 tgcgatagaa acgccagacc ggatgtctgt cagcaacatg attatccgtc cgacgacaca
     2221 ggaagtataa tccataacgg ataatatcca gagagaatta cttgttaaat agttattcaa
     2281 acgaccatca atttaaggcg tgaatggaga agaatattct tcatttacgt ctttttatgc
     2341 agaaaaagca aatttattaa aaagaaacaa atgttattta aatatggaca ttgacttgct
     2401 tttacttttt tagttacact gttatttagg aaaagtttca taattcaaag gagaaaaaag
     2461 atgaaagcaa agaatttatt atttagcgtt attcttttag gaaatctagc agttgccaac
     2521 acaacagtgc tagctgaaga atcgacagaa actgtcgcaa ctcagacatc gagttcttca
     2581 gaaacaggaa ttgaaaacga tacagaaagt actttgcagt cagctactgt ctcagaaaat
     2641 acaatttcaa ccgaagaacc aacaactgaa acaacagaac aaacacaaac cgaagacagt
     2701 acatcagcag aagctcagaa caatccagag aatgcagaag aaatcacgaa ccaattgaca
     2761 gatgggcaaa cattgacaca tacccatcaa gcagatttgt ctgaattcga aaactatact
     2821 ggctatgctt tttatgctcg ctcatctgct aattctcaac aagcgttcat tgatagtatt
     2881 gcctcaactg cacaaagttt ggcaagtgcc aatgacttat atgcatcagt catgattgct
     2941 caagccatcg ttgaaagtgg ctgggggaac agtactttgg cttctgcccc taattacaat
     3001 ctatttggga ttaaaggaag ctataatggc caatctgtta cgatgcctac ttcggaatat
     3061 gtgaatgggc aatggattac ggtgaatgcc gcttttagaa aatatccttc gtacaaagaa
     3121 tcattgcaag ataacgtaac ggtattgaag acgacttctt ttcagccagg cgtttactac
     3181 tattccggtg catggaaaag caatacgaac tcttacaaag atgctacggc ttggctaaca
     3241 ggtcgttatg ccactgcgcc taattatggt tctacattga acaatgtaat cgaaacctat
     3301 aatctgacac agtatgatac agcaccaact tcaacttctc cgatgtatcg tctgtataac
     3361 cgtcatacag gagaacattt atatacattg aatgccggag aaaaaaatta tctgccaact
     3421 gtaggctggg aatatgaagg gattgcttgg caagcaccaa acagcggaca acctgtctac
     3481 cgtttataca atccttacag tggagaccat cattacacaa tggcacaatc tgagatcaac
     3541 tctttaacaa aaatcggttg gcgttatgaa ggtctaagtt tttattctgg tggttccaag
     3601 ccaatttatc gactatttaa tccaaatgaa aaaactggta cccatcacta taccttgagt
     3661 gctaaggaaa gagattggct aacaccgatg ggctggagat atgaaggaat aggattttat
     3721 ggttattaaa aagaggtagt gaaaaagctg ccctgcatca agtagtaaga acagccaaac
     3781 aaaaacagat tttcatgttt tttgtttggt tgaacttatt accgcagatg caagtttttg
     3841 aacaccgttt actcaaaaaa ggttgtgaca acatcttgtc ataacttttt tattactgtt
     3901 taagcatact gatccaattt ctaatattcg acgggattct tgcaaaaggc gatttcggaa
     3961 atttttggcg gtgtaaaatt atctactgca gaagagctat cttctatagt ttgataagaa
     4021 tcaataatct tggttttagc gttgtgcttt ctttatcatt ttttccgttc attctacaaa
     4081 aaatttcttt tctctatact tttctattga gagcgttttt tttattacac agctgattct
     4141 ttgacttttt tcttttttta ttctattatt gacaagtcaa tcgttgacga gtcaattatt
     4201 agaaaggagg aaattatgtc tttagaagag attagtcacc ttttatatca aatcaaatta
     4261 gccgatcaaa aaatcgtcca gatctttgaa gaaaatatcg gtataagtct tactagatac
     4321 gagatcatga tgagactgaa agaagaaaat ccattgctac aaagcagttt gcaagaaaag
     4381 ttgcagattg atcaaggagc agttactcgt catctgaagg tattggaagc aaatggctat
     4441 gtttcgcgtc agagaaatcc tcagaacaac cgggaagtgt atgtagcgct aacagaaaaa
     4501 gcccagaaga aaatcaagca ttgtgaaatg gaccataaag atacaggttc cgtgctatcc
     4561 ccggctttca gcgatgtaga aatgaaacaa tttctaacac ttttagataa attgaatacc
     4621 caactttgca atataacaac agaaacgaaa gaggttaata ataatggaac aaaaaaatga
     4681 tttttcaaca attatgactg gtagaagatc cgttcgcgta tatgatgaaa catacaaaat
     4741 cccacacgaa gaaatgctgg aaatgatcaa agaagcgacg actgcacctt cttctgtcaa
     4801 tatgaaacca tggcgctttg tggtcgctga aagcgaggaa gcaaaagaaa tcttgcgtcc
     4861 tttgatccgt ttcaatacga gacaaaatga tacttcttct gcaatggtca tgatttttgg
     4921 cgatatgcaa tgttacgaat atggcgaaga aatctacaac caagcttacg aatcagggaa
     4981 gatgccaaaa gaagtcagag atcaacaatt agctgcgatc attccttatt acaagagttt
     5041 ttcaagagaa gaaatgaacg atgtagtcaa agtcgattct agcttagcgg ctatgcaatt
     5101 catgttagtt gcgcgtgatc atggctatga aacaaatcca attggtggat tcgaagcaga
     5161 tcagttggca gaggcatttg gtttggataa agaccgttat gttccagtta ttattttatc
     5221 aataggaaaa gcggtagaag aaggttacga gtctgttcgt ttagcaccag aaaagatcac
     5281 tatgttcgaa taaaaggttt tcagttacta ttcagtagct attgcttttt ttgaatcgtg
     5341 tgtggtaacc taataaaaaa ggaggtcgga aaaatgactg catcgatgaa attacgtttc
     5401 gaacttttga ataacaacta aataaattca aaagacatgt ataaacaaca atttcatgga
     5461 tggagtatgt ccattttttc gaacgacagt agaataggtg aaagctatga cagatttggt
     5521 gatttagtca tagttatttc gttcagagta ggaaaagatg tgatgcagtt tttttgcaaa
     5581 cagatttttc tgctcttttt tagtatcgat aattgttgtt ttacatgaaa taggagtgta
     5641 aaacatatgg aaaatttagc agtaaatata acaaatctac aagtcagctt tggcaaccag
     5701 ctagaactat ctattgattc tcttcgtgtc tatcagcaag atcgtatagg gatcatagga
     5761 gaaaatggtg tcggtaaatc gactttgctc aaactaatag ccggtgaact ttttcccgat
     5821 catgggaaaa tccaaacaga gatcaccttc aactacctgc ctcaattaac ctatctcgct
     5881 gaagcaaagg acctaaattt ggaattagcc agtcatttcc agttaagact ggaagaaact
     5941 tcagagcgga aatggagcgg aggggaagaa cgaaagatcg agttgatacg tcttctttct
     6001 tcttatgaac aagggatgct tctagacgag ccaacaaccc atctagatag aaaaagtatt
     6061 gatcgactga ttgaagagct tcgttattat tatggtacgc tggtttttgt tagtcatgat
     6121 cgctattttc tagatgagct agcatcgaaa atctgggaag taaaagacgg agaaatccga
     6181 gagttttcgg ggaattatag tgcctatctc actcaaaagg aattggagaa aaagactcag
     6241 ctacgagaag cagagtcgat catgaaagag aaaaaacgat tggaaaaatc gatccaagaa
     6301 aagaaaaaac aagcggaaaa gttggaaaaa gtgtccagta aaaagaaaaa gcaacaaatc
     6361 agactggatc ggttgtcttc ctctaaacaa aaagacagtg tacaaaaagc catccaaaag
     6421 aatgcgaaaa cattagagag aagactccaa aaaataggag aaacaaccaa gccgcagcag
     6481 atgaaacaaa tccgttttcc agtaccaaaa tctcttgaac tccacagccg ctatcccatc
     6541 atgggacaaa atgtccaatt ggaaagaagt gggagaacat tactggtaaa tggtgatttt
     6601 cagttttctt taggtaaaaa aatcgcgatt gtcggtgaaa atggttcagg taagacaacc
     6661 ttattagaac atatccgcaa acaaggagaa ggaatccttc tctctccgaa agtaagcttt
     6721 caagtatatc agcaaaaggg ttatcaaatg acatctgaag aatccatcat tcgttttgtc
     6781 atgagacaaa cagagttttc agaatcgctt gtccgtagtt tgctgaatca cttagggttt
     6841 gctcaggaaa ctttgacgaa accgttatgt acattaagtg ggggagaagc gacccgtctg
     6901 acgattgctt tgctttttac taagccaagt aatgtgttgc tgttagatga accgactaat
     6961 tttattgata tggcaacgat cgaagcttta gagaagctaa tgcaaatata tccgggaacg
     7021 attttgttta cttctcatga ttcttacttt gtcgagcgta cggctgatga agtttatgaa
     7081 ataaaagggc agaaaataaa aaaagtactt acgagaaatt tttaaatgtg ctacgatgat
     7141 tttcgtgaca aatttaaagg agtggtacag attgcctaca aataaaaaag aaggaataat
     7201 ttttacaaca atgatgtgtt ttttgatggt cttggggatg agcgcgtata atttatggct
     7261 gcatcaagat cttcatttat caagtttgtt gattggtcta gtgcctggat tcgtggtggc
     7321 tttcattttg gatgtcttca tcatcggtgt gctggcgaaa aaaattgcct ttaaactacc
     7381 agtaaatcca gaaagcaagg tccaaatgat cttaactatt tcatgcttga tggttttagg
     7441 aatggtgaca tgtatgtctt tattcggtgt gatcatggaa caaggaatac ctgatcaatt
     7501 attctctgct tatatgaatg cttggaaaat gaatgtgatt gccgctttac cgttgcagtt
     7561 gatcatcgtt ggcccgcttt caagaagtgt gctaaaagca attcaaagta atgaatgatg
     7621 gaacactgtt atttgaaaaa aggccttggc atgctttttg tcatagtctc ttgaatgcag
     7681 ttctagaaaa atagtgatcc gaacgatagg ttttggcctg tcgttcggat cgctttttct
     7741 tttccaactg tattgctctt ttattgaaga taatttgctc ttcaatagat atttctagaa
     7801 aggttctgtt tatcccttac tcaaaaaact gctgcatttg ttctcttact tgctgacacc
     7861 attggagttc ctgctgacaa agcataattc tgcgctggat cacaacataa cgtccaaatt
     7921 cttgtttctt gtctagcgag aggtcattca attttttttg atttttttgc atgattttgt
     7981 tcagctgttg ttctctagtg aatagcagtc cagtagctgt tgttttatcg agcgtcgaaa
     8041 aagcatagat ttttgctaga aattcatctt tttgcgtagg agcgggagtt tcctctttga
     8101 tccattcctc tacaagagca cgtccttcat cagtcaagtg gtagatcttt ttttgagagt
     8161 gctcgtcttt gtccaaaacg gtcacataac cctgctggtc taacttggca agcgttgtat
     8221 agatctgact gtgatgggct tcccaaaaaa ttgtgatcag ctgcttcaat tcatacccag
     8281 aacgtggttt ggataaaagc acgcataata atatataggc taaagtattc ataaaaatcc
     8341 tacctctcgt cttattttct ttattatatc aca
//
LOCUS       DAZKOW010000286         7810 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.118, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000286 DAZKOW010000000
VERSION     DAZKOW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.118"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..998)
                     /locus_tag="ACG912_003026"
                     /pseudo
     CDS             complement(1..998)
                     /locus_tag="ACG912_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            1489..2427
                     /locus_tag="ACG912_003027"
     CDS             1489..2427
                     /locus_tag="ACG912_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFQ0514238.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            2557..3204
                     /locus_tag="ACG912_003028"
     CDS             2557..3204
                     /locus_tag="ACG912_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HFQ0514239.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(3897..4835)
                     /locus_tag="ACG912_003029"
                     /pseudo
     CDS             complement(3897..4835)
                     /locus_tag="ACG912_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(4835..5452)
                     /locus_tag="ACG912_003030"
     CDS             complement(4835..5452)
                     /locus_tag="ACG912_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFQ0514240.1"
                     /translation="MAKIGYARVSSKEQNLDRQLQALQGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVIVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINSLVIELYKYQAESERKKIKERQAQGIEIAKKKGKFKGRQHKFKENDPRLKHAF
                     DLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVDQRKNNEKRDS"
     gene            complement(5466..5636)
                     /locus_tag="ACG912_003031"
     CDS             complement(5466..5636)
                     /locus_tag="ACG912_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514241.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            complement(5984..7474)
                     /locus_tag="ACG912_003032"
     CDS             complement(5984..7474)
                     /locus_tag="ACG912_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFQ0514242.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
BASE COUNT         2431 a         1328 c         1240 g         2811 t
ORIGIN      
        1 aatacaatat tgttgccaca cttaggacat tttcctacaa tacttttttc tgcttctttt
       61 tctttctgtt cctggtaatc agaaaaattt agtttttcta tatcgttagg tacagcttcc
      121 agtaaatgaa caatgaattt tttgatattc gtaataaagt tctcttgatt gccttctctt
      181 ttaccgattt tttttaaata cgtttcccat ttagccgtca tttcagcact cgttaaaagg
      241 tgctgacttt caactgcctg gcacaataat tttccttttt cagttacaac aagcttattc
      301 ttaatcactt ggatatattc tttttgtttt aacgcttcaa taatacttgc tcttgtcgct
      361 tctgttccaa tcccctcaac ttcttgtaaa atcttgattg cttcttcatc atcaaccgtt
      421 ttattcgccg ttttcatagc agttaataat gtaccctctg taaaagcttt tggcggttgc
      481 gtttcttttt caacactctt aacaccaact tcggcacgtt cgccaatgat aacgagtggt
      541 aacgtttgaa cgtcctcttt ttcttctttg gtttgttgtt tgaaaagaat tttccaccct
      601 tcttgctttg gtgtctttcc gatcgattga aacagcaaat cagctacttt cgtttgtatc
      661 ttggtttctt catacaagta atcaggtaaa aacatggcaa cggtcgtttt aacgactaaa
      721 gcataaattt ttcgttgtaa atcgtccatt ttcgctaatg cagattcggt aggtacttgt
      781 tttgttggga taatggcgtg gtgttcctgt accttactac cgtccacata acgctttcta
      841 ggctctgttt gaaccatttc aagatcaagt cctaaaaaac cgctatattt gccaaaattc
      901 gcttttaaat aagcaaattc gttctctgta ataaatggtg tatctgttct tggataactc
      961 aataattttg cttcatacag tccttgcata gcttttaaac gtaaaatttt gcgttctaag
     1021 acgttttaaa tatatctgcg tatattttta tgtgttttat cttttcgtcg gcaaatgcgt
     1081 taaaatttgc gaaataaaat agctcaaggg cgaacgcagt gagtgcattt acccttgatc
     1141 tatttttttg agtaaattag cttttgaaac cgacggaaag gaaacacaaa acttttcaga
     1201 ggcaaggggt ttttaagggg cgcttttagc cccttgaatt tatttgcttt aagatatatt
     1261 tagttttaaa atagttttaa cgaaaaaaag acataaaaaa agacgagcaa cgctaccaac
     1321 gttctcgccc ggaataacaa accacacttg ttattcattc gatgattagt atatcaattt
     1381 ttgattttga atacaaaaca aaatggacga atggatacag gcgaaattta ttttgcgatt
     1441 ccactcgtcc ttttttcgtt ccaaaaatta ttttaggagc gataaaaaat gaagaaaatt
     1501 gatgaaaaat tagctgcaga aatctcacta aaaaaaccgg ccgtctctaa tagccggtta
     1561 agtaatagcc ggttaagtgg tcaaactttg ggaaaatctc aacccgcatt aagttttgat
     1621 gccatgacaa tcgttggaaa tttgaacaaa actaatgcta aaaagctatc tgactttatg
     1681 agtgtagagc cacaaatacg actttgggat atacttcaaa caaagtttaa agctaaggca
     1741 cttcaagaaa aagtttatat cgaatatgac aaagtaaaag cagatacttg ggatagacgt
     1801 aatatgcgtg ttgaatttaa tcccaataaa ctcacacatg aagaaatgat ttggttaaaa
     1861 caaaatatta tcgactacat ggaagatgac ggttttacaa gattagactt agcttttgat
     1921 tttgaagatg atttgagcga ttactatgca atgactgata aagcagttaa gaaaactgtt
     1981 ttttatggtc gtaatggcaa gccagaaaca aaatattttg gtgtccgtga tagtgataga
     2041 tttattagaa tttataataa aaaacaagaa cgtaaagata acgcagatgt tgaagttgtg
     2101 tctgaacatt tatggcgtgt agaagttgaa ttaaaaagag atatggttga ttactggaat
     2161 gattgtttta atgatttaca catcttgaaa cctgcgtggg ctactttaga aaaaattaat
     2221 gagcaagcta tggtttatac tttgttgcat gaagaaagta tgtggggaaa gctaagtaag
     2281 aatactaaga ctaaatttaa aaaattgatt agagaaatat ctccaattga tttaacggaa
     2341 ttaatgaaat cgactttaaa agcgaacgaa aaacaattgc aaaagcagat tgatttttgg
     2401 caacgtgaat ttaggttttg gaagtaaaat aagttttatt tgataaaaat tgctaattca
     2461 gtataattaa tatttacgag gtgacataac gtatgaaaaa atcagaggat tattcctcct
     2521 aaatataaaa atttaaaatt taggaggaag ttatatatga cttttaatat tattgaatta
     2581 gaaaattggg atagaaaaga atattttgaa cactatttta atcagcaaac tacttatagc
     2641 attactaaag aaattgatat tactttgttt aaagatatga taaaaaagaa aggatatgaa
     2701 atttatccct ctttaattta tgcaattatg gaagttgtaa ataaaaataa agtgtttaga
     2761 acaggaatta atagtgagaa taaattaggt tattgggata agttaaatcc tttgtataca
     2821 gtttttaata agcaaactga aaaatttact aacatttgga ctgaatctga taaaaacttc
     2881 atttcttttt ataataatta taaaaatgac ttgcttgaat ataaagataa agaagaaatg
     2941 tttcctaaaa aaccgatacc tgaaaacacc ataccgattt caatgattcc ttggattgat
     3001 tttagttcat ttaatttaaa tattggtaac aatagcagct ttttattgcc tattattacg
     3061 ataggtaaat tttatagtga gaataataaa atttatatac cagttgctct gcaacttcat
     3121 cattctgtat gtgatggtta ccatgcttca ctatttatga atgaatttca agatataatt
     3181 catagggtag atgattggat ttagttttta gattttgaaa gtgaatttaa ttttatacac
     3241 gtaagtgatc ataaaattta tgaacgtata acaaccacat tttttggttg cttgtggttt
     3301 tgattttgaa tttggttttg aacttatgga ctgatttatt cagtccattt tttgtgcttg
     3361 cacaaaaact agcctcgcag agcacacgca ttaatgactt atgaaacgta gtaaataagt
     3421 ctagtgtgtt atactttact tggaagatgc accgaataaa aaatattgaa gaacaactag
     3481 caaaagattt taaagagtta ttttatttta agtctttata acatgagtga agcgaatttt
     3541 taaatttcga tagaaatttt tacatcaaaa agccccctgt caaaattgac gaagggggtt
     3601 ttttggcgca cgcttttcgt tagaaatata caagattgaa aatcgtgtat aagtgcgccc
     3661 tttgttttga acttagcacg ttacatcaat tttttaaaat gatgtataag tgcgcccttt
     3721 taaattttga gtgattatat tttttgagtt agaaaaaggg attgggaaaa tttcccaaaa
     3781 taatttaaaa aataagcaaa aattttcgat agagaatgtg ctattttttg tcaaaggtgt
     3841 ataccttgac tgtgcttgct gttacattaa gtttattttt aagttattaa aaaagaaata
     3901 gcttttaaag tttggctcgc tgtcgcttta taaagctgat tgacttttga ttgcaaacta
     3961 cttaaagaaa acaaactcgg actattcgtt ttcttctctt tggtttgaac atcagcaatt
     4021 atcccctctt gattgcctat tttagcttgt ttagaagaaa caaaagctaa aagctcctct
     4081 tgggttttaa aacgctgtgt ggggcttaga acgcccttaa acgacccttg gtttactttt
     4141 atactagctt ccacctcgaa aaaaggttct tttttaaaat tctctatggc ttcctggcgc
     4201 tgaaaaataa ggtataaggt gggcgtttga acacgtccta gtgaaaatgt accttgtacg
     4261 cccttctgtt gtaaatttaa cgtatacaaa gggcttgcgt tcatgccgat caaccaatcg
     4321 gcaatttggc gtgtttgcgc ttcttgataa aagggatagt aattcattcc aggttgcaaa
     4381 ttttgaaaac cgcttcggat tacatctttt tctaagctat tgatccatag tcttttaaat
     4441 gttttatctt ttgaaaaggc atttgcttta tggataatcg accaggcgat attttcacct
     4501 tctctgtcgc tatctgttgc aacaataatt gtatttgcct ttttgagaag ttctgcaaca
     4561 attttaaact gctttccctt atcttttgca acttcaaaat cgtatcgatc aggaaaaatc
     4621 ggcaaagatt caagtttcca attttgccac ttttcgtcat aatgacctgg ttctgctaat
     4681 tccactaaat gcccaaaacc aaaggtgata aacgtttcat ctgtaaatag tgggtctttg
     4741 atctcaaaat aaccgtcttt tttggtgctt tgttttaaag cacttgcgta ggctaatgcc
     4801 tggcttggtt tttcagctaa aataaccgta ctcattaact atccctcttt tcattgtttt
     4861 ttctttgatc gactgtcacg ttatatcttg ctcgatacct tctaaacgtt cggcgattga
     4921 ttccagtttg ttcttcaact tctttatcgg ataaaccatt caaaaacaaa tcgaaagcat
     4981 gctttaaacg tggatcattt tctttaaatt tatgctgacg acctttgaat ttgccttttt
     5041 tcttagcaat ttcgattcct tgtgcctgac gttccttaat tttttttcgt tctgattctg
     5101 cttgatactt gtacaattca atgacaaggc tattaatcaa acgccttaaa ttttcatctt
     5161 caataccatt cattgagggt aaatttaaga cttccagggt tgccccctta atttgaattt
     5221 gattcatcaa ttctgttaat tctttattat ttcgtcctaa tcgatctaat tcagtaacaa
     5281 taacaatatc cccttcacga atatagttaa gcatagcttg taattgtggg cgttcgaccg
     5341 attgaccgct taatttgtct gaaaagacct tagaaacgcc ctgtaacgct tgtaattgcc
     5401 gatctaagtt ctgttctttg ctactgacac gtgcataacc aattttagcc attttcaacc
     5461 aacctctaaa attctctcgg ttgcaataac caatcagcaa tatctacttt ttcaatttca
     5521 aattgcttat cagaaattgt cttttcgtaa gcgataaaat cttgcgcata ttgttgctca
     5581 ttaaaaatag ccaccacttc gtcattttct aaaactcgat aaataaattt tttcatttta
     5641 ctcctcctat tatgcccaac ttaaatgacc tattcaccaa gtcaattata ctgctaaaat
     5701 catattagga caaataggta tactctaatg acctataaaa gatagaaaat taaaaaatca
     5761 agtgttcgct tcgctctcac tgcccctcga cgttttagta gcctttccct cacttcgttc
     5821 agtccaagcc aactaaaagt tttcgggcta ctctctcctt ctccccctaa taattaatta
     5881 aaatcttact ctgtatattt ctgctaatca ttcgctaaac agcaaagaaa aaacaaacac
     5941 gtatcataga tataaatgta atggcatagt gcgggtttta ttttcagcct gtatcatagc
     6001 taaacaaatc gagttgtgtg tccgttttag ggcgttctgc tagcttgttt aaagtctctt
     6061 gaatgaatgt atgctctaag tcaaaagaat ttgtcagcgc ctttatatag ctttcttttt
     6121 cttctttttt tactttaatg atcgatagca acaatgattt aacactagca agttgaatgc
     6181 caccatttct tcctggttta atcttaaaga aaatttcctg attcgccttc agtaccttca
     6241 gcaatttatc taatgtccgt tcaggaatgc ctagcacttc tctaatctct tttttggtcg
     6301 tcactaaata aggcttgtat acatcgcttt tttcgctaat ataagccatt aaatcttctt
     6361 tccattctga caaatgaaca cgttgacgtt cgcttctttt tttcttgaat ttaaaccacc
     6421 cttgacggac aaataaatct ttactggtta aatcacttga tacccaagct ttgcaaagaa
     6481 tggtaatgta ttccctatta gccccttgat agttttctga ataggcactt ctaacaattt
     6541 tgattacttc tttttcttct aagggttgat ctaatcgatt attaaactca aacatattat
     6601 attcgcacgt ttcgattgaa tagcctgaac taaagtaggc taaagagagg gtaaacatga
     6661 cgttattacg ccctattaaa cccttttctc ctgaaaattt cgtttcgtgc aataagagat
     6721 taaaccaggg ttcatctact tgttttttgc cttctgtacc gcttaaaacc gttagacttg
     6781 aacgagtaaa gcccttatta tctgtttgtt tgaaagacca atcttgccat tctttgaaag
     6841 aataacggta attaggatca aaaaattcta cattgtccgt tcttggtatg cgagcaatac
     6901 caaaatgatt acacgttaga tcaactggca aagactttcc aaaatattct cggatatttt
     6961 gcgaaattat tttggctgct ttgacagatt taaattctga ttttgaagtc acatagactg
     7021 gcgtttctaa aacaaaatat gcttgataac ctttatcaga tttgataatc atagtaggca
     7081 taaaacctaa atcaatagcg gttgttaaaa tatcgcttgc tgaaatagtt tcttttgccg
     7141 tgtgaatatc aaaatcaata aagaaggtat tgatttgtct taaattgttt tcagaatgtc
     7201 ctttcgtgta tgaacggttt tcgtctgcat acgttccata acgataaacg ttgggtgtcc
     7261 aatgtgtaaa tgtatcttga ttttcttgaa tcgcttcctc ggaagtcaga acaacaccac
     7321 gaccgccaat catgcttgat tttgagcgat acgcaaaaat agcccctttg cttttacctg
     7381 gcttggtagt gattgagcga attttactat ttttaaattt gtactttaac aagccgtcat
     7441 gaagcacagt ttctacaaca aaagggatat tcattcagct gttctccttt cctataaatc
     7501 ctataaaata ggttgtttaa ttaacttggt ttgctttttc attcaactgt ttcaatattg
     7561 catgttttga aaaagatttt tttcctttat aagtcaattt ttttccacta atcgaataaa
     7621 ttattttgtt attttctatt aacttatata tataatcttc cccctccgaa gaaaaatact
     7681 tatctgattt tgtttctaag tagatatttc tcttttctaa ctctttctta aacgtttcta
     7741 gtgtatagat atttgctaat tttcttatct ccaagtcaag tccataatcc tgtgtaaaat
     7801 actatacaat
//
LOCUS       DAZKOW010000287         5682 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.130, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000287 DAZKOW010000000
VERSION     DAZKOW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.130"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            164..211
                     /locus_tag="ACG912_003033"
                     /pseudo
     CDS             164..211
                     /locus_tag="ACG912_003033"
                     /inference="COORDINATES: protein motif:HMM:NF011335.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            227..2146
                     /gene="tet(M)"
                     /locus_tag="ACG912_003034"
     CDS             227..2146
                     /gene="tet(M)"
                     /locus_tag="ACG912_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HFQ0514243.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2265..2432
                     /locus_tag="ACG912_003035"
     CDS             2265..2432
                     /locus_tag="ACG912_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFQ0514244.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2492..2845)
                     /locus_tag="ACG912_003036"
     CDS             complement(2492..2845)
                     /locus_tag="ACG912_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514245.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            3349..3768
                     /locus_tag="ACG912_003037"
     CDS             3349..3768
                     /locus_tag="ACG912_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFQ0514246.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            3794..4000
                     /locus_tag="ACG912_003038"
     CDS             3794..4000
                     /locus_tag="ACG912_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514247.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(4228..4401)
                     /locus_tag="ACG912_003039"
     CDS             complement(4228..4401)
                     /locus_tag="ACG912_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514248.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            4413..5042
                     /locus_tag="ACG912_003040"
     CDS             4413..5042
                     /locus_tag="ACG912_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514249.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            complement(5124..>5682)
                     /locus_tag="ACG912_003041"
     CDS             complement(5124..>5682)
                     /locus_tag="ACG912_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HFQ0514250.1"
                     /translation="GKEIKEPCPTCHGSGHEKKAHSVKVNVPAGVEEGQQMRLAGQGE
                     AGENGGPFGDLYVVFRVEESDIFDREGSEIYYELPLSFVQAALGDEVQVPTVHGDVKL
                     KIPAGTQTGTNFRLRGKGAPKLRGGATGDQHVKVKLITPKNLNEQQREALRAFAEAGG
                     IKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         1846 a          969 c         1068 g         1799 t
ORIGIN      
        1 taaactgtca atttgatagt gggaacaaat aattggatgt ccttttttag gagggcttag
       61 ttttttgtac ccagtttaag aataccttta tcatgtgatt ctaaagtatc cggagaatat
      121 ctgtatgctt tgtatgccta tggttatgca taaaaatccc agtgataaaa gtatttatca
      181 ctgggatttt tatgcccttt tgggtttttg aatggaggaa aatcacatga aaattattaa
      241 tattggagtt ttagctcatg ttgatgcagg aaaaactacc ttaacagaaa gcttattata
      301 taacagtgga gcgattacag aattaggaag cgtggacaaa ggtacaacga ggacggataa
      361 tacgctttta gaacgtcaga gaggaattac aattcagaca ggaataacct cttttcagtg
      421 ggaaaatacg aaggtgaaca tcatagacac gccaggacat atggatttct tagcagaagt
      481 atatcgttca ttatcagttt tagatggggc aattctactg atttctgcaa aagatggcgt
      541 acaagcacaa actcgtatat tatttcatgc acttaggaaa atggggattc ccacaatctt
      601 ttttatcaat aagattgacc aaaatggaat tgatttatca acggtttatc aggatattaa
      661 agagaaactt tctgccgaaa ttgtaatcaa acagaaggta gaactgtatc ctaatatgtg
      721 tgtgacgaac tttaccgaat ctgaacaatg ggatacggta atagagggaa acgatgacct
      781 tttagagaaa tatatgtccg gtaaatcatt agaagcattg gaactcgaac aagaggaaag
      841 cataagattt cagaattgtt ctctgttccc tctttatcat ggaagtgcaa aaagtaatat
      901 agggattgat aaccttatag aagttattac taataaattt tattcatcaa cacatcgagg
      961 tccgtctgaa ctttgcggaa atgttttcaa aattgaatat acaaaaaaaa gacaacgtct
     1021 tgcatatata cgcctttata gtggagtact acatttacga gattcggtta gagtatcaga
     1081 aaaagaaaaa ataaaagtta cagaaatgta tacttcaata aatggtgaat tatgtaagat
     1141 tgatagagct tattctggag aaattgttat tttgcaaaat gagtttttga agttaaatag
     1201 tgttcttgga gatacaaaac tattgccaca gagaaaaaag attgaaaatc cgcaccctct
     1261 actacaaaca actgttgaac cgagtaaacc tgaacagaga gaaatgttgc ttgatgccct
     1321 tttggaaatc tcagatagtg atccgcttct acgatattac gtggattcta cgacacatga
     1381 aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac tgttgcaaga
     1441 aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg agagaccgtt
     1501 aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct gggcttccat
     1561 tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga gctcggtttc
     1621 tcttggatac ttaaatcaat cgtttcaaaa tgcagttatg gaggggatac gctatggctg
     1681 tgaacaagga ttgtatggtt ggaatgtgac ggactgtaaa atctgtttta agtatggctt
     1741 atactatagc cctgttagta ccccagcaga ttttcggatg cttgctccta ttgtattgga
     1801 acaagtctta aaaaaagctg gaacagaatt gttagagcca tatcttagtt ttaaaattta
     1861 tgcgccacag gaatatcttt cacgagcata caacgatgct cctaaatatt gtgcgaacat
     1921 cgtagacact caattgaaaa ataatgaggt cattcttagt ggagaaatcc ctgctcggtg
     1981 tattcaagaa tatcgtagtg atttaacttt ctttacaaat ggacgtagtg tttgtttaac
     2041 agagttaaaa gggtaccatg ttactaccgg tgaacctgtt tgccagcccc gtcgtccaaa
     2101 tagtcggata gataaagtac gatatatgtt caataaaata acttagtgta ttttatgttg
     2161 ttatataaat atggtttctt gttaaataag atgaaatatt ttttaataaa gatttgaatt
     2221 aaagtgtaaa ggaggagata gttattataa actacaagtg gatattgtgt cctgtatgtg
     2281 gaaataaaac acgattaaag ataagggaag atactgaatt aaaaaaattc cccctctatt
     2341 gtccgaaatg cagacaagaa aatttaattg aaataaagca gttcaaagta actgtgatta
     2401 cagagccaga cgcaaagacg cagagccgat aaaatgagat taatacaatc tcattttatc
     2461 ggctctttcc gttatgtatg gattctttta attagtcttc gatgtttctt gcttcgttga
     2521 taccgctggc taaagattcc attaaggata gttctttgtc tgtaaagcta tccatgtatt
     2581 tctctatctg taatcgtcgg gtgcttttta ccaagttatt agcaggtaag aaaaattcat
     2641 caacggaaac atgaagtaac gatacaaggt cataaagaac ttgtatgctg gggtgttgcc
     2701 ctttattttc aatattagtt aagtaccgtg ggtcaatttc aatcaatgct cccacttgtt
     2761 cacgagttaa acctcgtttc aatcgagctt ctttaatggc taaaccaaag gctctaaaat
     2821 catatttatc ttctttttta cgcatagtag accacctcta tacattttat tgttcctact
     2881 gaattaaaaa caggtataga aaaacgtgtt atatggttta taggtttata tttaataaaa
     2941 agcactacta aacgccaata aaaaaaaccg ttatatggta gtgctattta cgctgttaaa
     3001 atattgtata ttacttccaa atggcggttt gttggaggtc aacgtcgcca tgaagtacat
     3061 catatacaat aaatttcctt acattgggtt cttgtcaaaa aaagtcgtct atctgcaata
     3121 gataagtacg tccaccaatg tggttttata aatcatatag atagaataac agaagcatgt
     3181 aaacagagaa ataaatctgt ttatatgctt ttttggctat tcagaacttt tttacaaagt
     3241 ttatttatca gtaatgcaac aaatccccct ttcacattgg gactaagagt gaaaggagat
     3301 aaacgagcaa ggctcacttc ctttcctaga cagaaagggg gtgagattat gaggacatct
     3361 tctttcgagc atatcgtcag attacagttc aattctttaa tgctgattgt cattaagaac
     3421 aaagtgaaaa gtcgaaacag acagcttgca agacattcta aacgtgaagt attgttttgt
     3481 gaaatagccg aaacgaaaca agttgaacgt ggaacgattg atacctgttt ttatgattgt
     3541 gtatcattta aagtcttgca ctttacaatt tatgtatctg atgaaacact tggtacagtt
     3601 ttacatagac tatcagaaaa acagcgtagt gcgattttat tacgttattt tcaaggaatg
     3661 aacgacagag aaatttcaga actgtaccat gtatcacgtt ctgctatttg cagtagaaga
     3721 actagaggac taaggaagtt aaaaatgctt ttgagcgaaa ggaagtaaag attatggcta
     3781 tccctctttt gaattgattt gcaaagcgtc aagcggagat gaaatggcag ttaaagagat
     3841 tttgaagttt tatgacggtt atatttctaa attatgcttg cgtccttttt accactctga
     3901 aagtggtaaa atcattatgc aggttgatga agaactaaaa ggagaaattc atacagacat
     3961 gatgaaagca atattgaaat ttgaaatcgg agtgaagtaa tcaactgtat aaaaaaatcg
     4021 ggagatacac agtttcgtgt tatctccctt agtagtttta cggtcatatc aaggctcatt
     4081 caatcgtggt aaattcgtgg taagatgagt gtttttctat acttcaaaat gcttgaatgt
     4141 atagtgttta tgcggataat tgaaaattag atataaaatt taataaaaac caaaaatgca
     4201 cggtcgccat gaagggaccg tgtattttta tcttcttatt ttctcaccta atcgtatcag
     4261 ccgcatcgct tgttttcctg cacaaacaag caatcgctct acctgttcca tcgcataaga
     4321 aagtaccatc ggttcattga tgatatcaag tacatagtcg ataccaccct cacttaattg
     4381 agcttgattt ttcttctcca tattatgtag ttgtgggaca actttctgac aatcagcaat
     4441 tgtgtcaaga acggatcaat cctttattgg agttgctcga aagggtcttc tcttattacg
     4501 gacaggcttt gctcactgta catagacaac aaatcattat cctagtcaat cggatttcca
     4561 gagcctcatt gctatcattg cttgataaaa tccagacaaa gtttaataaa atgtatcagt
     4621 tacagctaaa ctttggaatt gggtcgcttt gttatacaga gcaagagaca ccgcaatctt
     4681 tccttcatgc caaacaagtt tgcgaatgga tagctttcca tcaatctgtg aacgagatcc
     4741 gcttttttga agatttagac ctcgggatcg ttttaccagc aatcccgtca gatcaacgaa
     4801 cattatatgt aaaacgtatt ttgaaaagcc tgacagaaga agaagtacat ttgttcaaga
     4861 agactttggc atgcttcagt aaaaataacg gaagcatcaa aaattgctca gaagaattat
     4921 ttattcataa aaacaccttg caatatcgat tgaacaagtt ccattctctc actggttata
     4981 cccctagaaa ctatgacgat tatcatattc taaagctggc ttttttgttg gtacaaacat
     5041 gacaactaca aaaaaaccag gcgaacatat gattgttcgt ctggttttta attattaatg
     5101 acttagcgtt tttactagaa aaactacttc cgctttttcc cgccaaaagc atctttaaac
     5161 ttatcgaaga aaccgtcttc ttgctgttct tcaactttga tgccgccagc ttcagcgaat
     5221 gcacgtaatg cttctctttg ttgttcattt agatttttcg gtgtgatcag ttttactttg
     5281 acatgttggt cgcctgttgc accaccacgt aacttcggag cgccttttcc acgtaaacgg
     5341 aagttcgtac ctgtctgtgt tccagcaggg atcttcaatt tcacatctcc atggacagtt
     5401 ggtacttgga cttcatcccc caaagctgct tgtacaaaac ttaaaggcaa ttcatagtaa
     5461 atttcagaac cttcacgatc aaagatatca ctttcttcta cacggaagac cacatacaaa
     5521 tcaccaaatg gtccgccgtt ttctccagct tctccttgac cagctaaacg catttgttgt
     5581 ccttcttcta caccagctgg tacattcact ttaactgaat gtgctttctt ctcatgtcct
     5641 gagccatgac aggttggaca aggttctttg atttctttcc ct
//
LOCUS       DAZKOW010000288         5666 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.131, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000288 DAZKOW010000000
VERSION     DAZKOW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.131"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            137..829
                     /locus_tag="ACG912_003042"
     CDS             137..829
                     /locus_tag="ACG912_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFQ0514251.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1156..1653
                     /gene="dfrG"
                     /locus_tag="ACG912_003043"
     CDS             1156..1653
                     /gene="dfrG"
                     /locus_tag="ACG912_003043"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFQ0514252.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1725..3677)
                     /locus_tag="ACG912_003044"
     CDS             complement(1725..3677)
                     /locus_tag="ACG912_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFQ0514253.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3684..3920)
                     /locus_tag="ACG912_003045"
     CDS             complement(3684..3920)
                     /locus_tag="ACG912_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514254.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4322..4612
                     /locus_tag="ACG912_003046"
     CDS             4322..4612
                     /locus_tag="ACG912_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFQ0514255.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(4884..5233)
                     /locus_tag="ACG912_003047"
                     /pseudo
     CDS             complement(4884..5233)
                     /locus_tag="ACG912_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5326..5628
                     /locus_tag="ACG912_003048"
                     /pseudo
     CDS             5326..5628
                     /locus_tag="ACG912_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
BASE COUNT         1928 a          905 c          987 g         1846 t
ORIGIN      
        1 gataaaaaag gaggattaaa tgagtatttg atagtattta tattaaaatt acatatgtaa
       61 tttaaaaacg cttgacattg tgtttgagat cacttacttt tagcatgtaa aagtattgga
      121 ttaaggagct atgcaaatga aaaagaaatt tattactggt gggattgtcg ttgcatttct
      181 atgtctaatt ggctacacag gcacgcggta cgcaaatgaa attattttgt ggggtggaga
      241 gacagatatt gatacaatca atgagaatct gacacagctt gatcatacat tgacagataa
      301 agagcaagcg attacagatc ttaccgctcg actagcacaa aaagaacaga cattgcaaga
      361 ttcaaaggca gatgtccaag aataccaaac aaaagtaaat gaattagaaa accaaaaaaa
      421 tcaatcggca gcagaaaaac aaaacttaga aagtcaatta gtcagcaagg atgcacaact
      481 acaaagcaaa caaaatgaaa tcaatgtcaa actagatgag atcaaccaaa aaaatgatga
      541 aatcaatcat ctgaatgcag attggacagc aagactacaa gaagcacaag caaaactttc
      601 agaattacaa aatactgtaa atggtttgaa tcaacaaatc gcaacactga cacaagaaaa
      661 aacaaatgta gaaactcagt taacagatac acaaaagaaa ttagacgaag caactgctga
      721 aaactcaagc ttgaaacaat atattcaaaa gttagaaaaa gcaaaagatg aagtggaaga
      781 tacagctgat aaatctcagc aaattgttga agaacatttg cgtaaataat ctttcttaac
      841 aagtaaacct aaattagtaa agaagcaaca gtaatctata ctaagtagtt cagttttgga
      901 gtacaaaatg acaaaatgta cttcaaaatg gactactttt ttcattttaa cttacatacc
      961 aaattacata caaaatgatt tataaaatgc aattgaaaat gataattgaa tgaaaatttg
     1021 tgattttgtg gtattatgat aatataactt atagaaaaaa ttgcttattc aactaacggg
     1081 caggataatg agaaaaggtt ttttgataga aataagaaat tagatggtaa attagttaaa
     1141 aaggctgtgt tataaatgaa agtttctttg attgctgcga tggataagaa tagagtgatt
     1201 ggcaaagaga atgacattcc ttggaggatt cccaaggact gggaatatgt taaaaatact
     1261 acaaagggac atccgataat attaggtagg aagaaccttg aatcaatcgg aagagcctta
     1321 cctgacagaa gaaatattat tctgacgaga gataaggggt ttacctttaa tggttgtgaa
     1381 attgttcatt caatagaaga tgtttttgag ttatgtaaaa acgaagaaga aatttttatt
     1441 ttcggaggag aacagattta taatttgttt ttcccttatg ttgagaaaat gtacatcaca
     1501 aaaatacatc atgaattcga aggagatact ttttttccag aagtgaatta tgaggaatgg
     1561 aatgaggtat ttgcccaaaa agggataaag aatgataaaa atccgtataa ctactatttt
     1621 catgtatatg aaagaaaaaa cttattgagt taacgggtgc gttaattgaa gaagaacagt
     1681 aaagataaag cacaagagct tgggattact ccaagctctt tttgtcattt gaaataaatt
     1741 atatctttcc aatcaaattg tttatctgta atccctaatc tttgagcagg tgtcaaaacc
     1801 tttttatccg ctgatttaaa agggcgacaa aagttgtagt aagttcttag aattgtaatc
     1861 gcaaattgag catacttcgg attgaagtta gcgtaaatat agctcttctt atcaccacga
     1921 gctgttgtta atggtctttc aagagatgat atataacgtc ttatctgttg aataaagcta
     1981 ttggtcgaat ggtcattaac atttaatagc atctttgcga tttcgtttgg ttccagagca
     2041 gataaatccg ttctacaatc aactgaataa aatcctttat ccttagtggc taatggatgt
     2101 ttgatagggt tatcggcata tttgcgatat ctttctccat ctttactaat agcctcttcg
     2161 tggaattgat gacttgttag taattcagtt agataatgag atgccaaagt ccgtagaaac
     2221 tttgttttga atcctctgat atctccccaa tcgagcagtt ctgcttttgc ttgtccgaat
     2281 tcttttaagc attgttttct gcttttcgtc ttgttaactt ggcaaataaa atgatgtgca
     2341 tcgctcaacc ttaattcttt cgtgaatact ctataaaagg cgtttctaat agagaagtca
     2401 tcatcactaa tcattcgcca ttcgtcggca ttaaccattt gtttaattaa ccaatagtga
     2461 gcagttgtag tataaggagc atcaacattt aaaccatcga caaatctact cctattagta
     2521 attttcccta attcgtgaag ataagcattc ctattttcgc tatcgttagc ggatggttct
     2581 tgtggataat aagaccaatc taacctatca tttttacgac taaaagtatt gagatggtct
     2641 tctttgaatt ttcttgtatc ttcatttaat tcatccattg aaatattcca atcataggca
     2701 acatcagaac ggaacacata tcttgataag acctcagcag atacaactat atacgtttgc
     2761 atattaaggt cttcaaaccc cgtatatttc tttgaacctt gtcctttctt acggacgtta
     2821 tttaggtagt agtgcatctt gtccgtatta agccacatct cgttaaattt ctttgtttga
     2881 aggggttgtg tctcatatcg ttctaaaaac tccaaacaac gcctatacag ccactccagc
     2941 ttatgatagt aggttccaac tccaattcct aaaatatcac aagtacgact gactgaaacc
     3001 cttccaacaa ccatcttagc gaacattgga agaatggtgt tcttttgttg gtgataagta
     3061 gtggtttctt ctctttttgg tagaacatta gtgaacttct tacacgcctt acattgatag
     3121 cgttgagatt tgcccctact tttccccctt ttgtaaaatt gcttcggctc attgaatggg
     3181 gttgattctt cttccgagca gccttcttta tgaaattgat aatctggctc aacatcttgg
     3241 atgctattga tttctataag gcgtttaatt tcttcaacaa cagaccaatt agataaaggg
     3301 gttactgtac agttttgcga tacacctctg ccaataggat tgtcattgca gtacatacct
     3361 ttatgacctt tatctttgga actaccagtc attttatatc gacttggttt cccttttaca
     3421 tctttgaatt tgtgctgttc tttcccaaaa ttacaacaaa acggattgat gcagtaatta
     3481 tattgaatgg gatattcaga accgttccat ttaaaagacg ttggcaagaa caacattttg
     3541 ttataccttg ttgcaaatcg ttttggagtt aataaatcat attgtttagc cctatctttt
     3601 aattcttcgt ttgaaatcgg aattttaaca tctacaattt catcttcctt cgtggctaat
     3661 cttttaagct tggtcattgt ctatcaaccc actctatctt tgatacccat tttttcaaca
     3721 atccgcttat tacttcgctt gttggcaatc cattcaataa acttcttatc gtctaaaatg
     3781 ttcaataaga attcatccac tgcttcgctt tcagtacgct ctgcatattc agcgtattgc
     3841 ttaactatca cccttacttg ctctgatatt tcccaatcca cagacttagc gttcttattt
     3901 tttggctcaa gaaattgcat aggaaaatgc accccttttt gcttagtaaa ttgtaaccat
     3961 tttaacacac caatttgcat tcctacaata attatttcac gttcgcattt ttatatacat
     4021 tttaacttac attttgtggt gtaaattaac aacaaacgtt gatttaacaa taaaaaacga
     4081 gtatcaaatt cagttgcaat ttgatactca tttaggttta cattttcact ttttgtactc
     4141 caaaactgaa ctacttaaat ctatactgct gcctcttttt tttagtacat gtccttcatc
     4201 ttcgcaggaa gtgccaatat gattttaaga aaggtaatag acaaaaagtc aaaaagataa
     4261 aacagagttt cttctttaca aagggaatgt atgttcgtat aatggtattg aggtgaaggt
     4321 tatggagaaa aaatatggaa cagtagataa gatccgtact ttgcaagtta gtgccagacc
     4381 gttgatccgt ttcagtttaa atggggtcaa ttgtttgatt gcttcccata gtttgaactt
     4441 cctagctgaa gtagacgaag ggatgaagtt agtcgttaca ggttattata ataatagaaa
     4501 gcaatttgtt gtgagatcct atcatgtgtt agggaaaccc aaaattgtgg tagaatatga
     4561 gaaaagtcta tacccaagaa aaaaggtccg acaagatgaa agaataaact agaaaacaag
     4621 cggctgagac aaaaatgtcc tagccgcttg ttttggaaag atggatgcag acgatgggat
     4681 ttgaacccac acgagcatac gctcacaaga acctcaatct atatataact acatgtattt
     4741 aagtatttgt gatgaatcac taaactttcc aaactcattt gagggatatt gcttaaagat
     4801 tgaacagatc aattcgaagc cctattagta ttcgctcaat ggggggatca gaggccataa
     4861 aaagagacta gtcattacta gtctcaggta gtggaattgt aggattttaa acgttcctgg
     4921 agagtaccgg tatgtaactc gaagaggtga ttatcatagt cgtaaaagta aattgaagaa
     4981 gcctcgcctt tcacacgaga acgtcctggt tcgacagtta aacctaacat ttggattttt
     5041 gagacaaacg aatctatatc ttgttcgtca attttaaaag caatatgatt gtatgtcttt
     5101 ggcagtttat tcgacgaatt ctgcattact gcaatccata ggtccttaac taagaaaaat
     5161 ttttctggat ataatgaaaa tttttttgtt gactatcgta gatttctttt gcatcaaata
     5221 aggtatttaa caattaagta tttaaaagat aacttttaag ttatctttaa gtaatgctta
     5281 aagctatgtt atactctatt aggaaatata agaaggagta taaaaatgaa caaacataat
     5341 agaaaaataa cgaataacaa agaactgatc ttgttactaa tagggatttg gggtatcttt
     5401 ttactcttta atatgtatat gccgactatg agagcggatg acgtagttta tgcaagtaga
     5461 ttagatgaac ttggctattt gggtgcttcg atagaacatt ataagacatg gagttcaaga
     5521 attattatag aactattctt aatgttcttt tctaaacact ttatgctatg gaaattatta
     5581 aattctacga ttatgttagg aattgtagta ttactttgca agtatgttga gagtataaaa
     5641 tattttgtgt aaatgaaaaa atccat
//
LOCUS       DAZKOW010000289         3278 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.165, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000289 DAZKOW010000000
VERSION     DAZKOW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.165"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..188
                     /locus_tag="ACG912_003049"
     CDS             3..188
                     /locus_tag="ACG912_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFQ0514256.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="ACG912_003050"
     CDS             203..1072
                     /locus_tag="ACG912_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFQ0514257.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="ACG912_003051"
     CDS             1053..1787
                     /locus_tag="ACG912_003051"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFQ0514258.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="ACG912_003052"
     CDS             1820..2728
                     /locus_tag="ACG912_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFQ0514259.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3205
                     /locus_tag="ACG912_003053"
                     /pseudo
     CDS             2725..3205
                     /locus_tag="ACG912_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1146 a          465 c          718 g          949 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagaattgga tgataaaaat agaaacaacc ttgttgtgcc
      421 tcccggagcg tggggtgatt ggattaatgg cggcggatgg ttatttataa atgggtatca
      481 tgttgacttg attttacgtg atataaaacg ggtagaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgcaatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggtag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgatatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     3001 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     3061 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     3121 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     3181 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtg
     3241 gttctgttgc aaagttttaa ataaagaata aaatccct
//
LOCUS       DAZKOW010000290         2839 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.178, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000290 DAZKOW010000000
VERSION     DAZKOW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.178"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..308
                     /locus_tag="ACG912_003054"
     CDS             <1..308
                     /locus_tag="ACG912_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFQ0514260.1"
                     /translation="EKKIEQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAI
                     CGSRDQELEGLSDFLPIVLSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            566..2068
                     /gene="eat(A)"
                     /locus_tag="ACG912_003055"
     CDS             566..2068
                     /gene="eat(A)"
                     /locus_tag="ACG912_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFQ0514261.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            2135..2662
                     /locus_tag="ACG912_003056"
     CDS             2135..2662
                     /locus_tag="ACG912_003056"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFQ0514262.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT          981 a          491 c          610 g          757 t
ORIGIN      
        1 tggaaaaaaa gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc
       61 aaacgatcca cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac
      121 caataatcgc tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc
      181 caattgtctt aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc
      241 aaacattaga aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc
      301 cgaagtagtt gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt
      361 gagggatccg tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct
      421 tgtggagaca aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg
      481 ggtctttttg tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg
      541 gtcttttttt acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg
      601 ctacgacagc caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg
      661 gaaactagga cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca
      721 aaacaaacta ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca
      781 acagacaaaa gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga
      841 gatatgggaa atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag
      901 agaattcagc acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt
      961 ggatgacact catttcccgt taatcgatga accaacgaat catttggata tctctggtag
     1021 aaaacaagta gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga
     1081 ccggggattt atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga
     1141 actttatcaa gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga
     1201 aatggctcaa aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga
     1261 aaaagccgaa tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat
     1321 cgatactgaa tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat
     1381 gaaacgatcc aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact
     1441 attaaaagat atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa
     1501 gcgactttta agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat
     1561 tcattttaca atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa
     1621 gtcatccatt atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata
     1681 ccagccaaaa catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac
     1741 gttggcggaa tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg
     1801 aaagcttggg atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca
     1861 acggaaaaaa gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga
     1921 tgaaccattg aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa
     1981 cgtgaaacct gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac
     2041 tgagattatt tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt
     2101 taaggggaac tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga
     2161 aacaacttgg aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa
     2221 aggacagatg caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac
     2281 gaaaatcatc cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg
     2341 tctcagcttg ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga
     2401 tggactgcca agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt
     2461 cccaaatggg gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg
     2521 catcgaagac gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat
     2581 caatgcttat tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac
     2641 agaattggtt tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc
     2701 actgcttttg cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact
     2761 caacctttag aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag
     2821 atatatactc tagtcatag
//
LOCUS       DAZKOW010000291         2546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.182, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000291 DAZKOW010000000
VERSION     DAZKOW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.182"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..785
                     /gene="vanR-A"
                     /locus_tag="ACG912_003057"
     CDS             90..785
                     /gene="vanR-A"
                     /locus_tag="ACG912_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFQ0514263.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            763..1917
                     /locus_tag="ACG912_003058"
     CDS             763..1917
                     /locus_tag="ACG912_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFQ0514264.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2132..>2546
                     /locus_tag="ACG912_003059"
     CDS             2132..>2546
                     /locus_tag="ACG912_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0514265.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDS"
BASE COUNT          802 a          494 c          556 g          694 t
ORIGIN      
        1 tggcctttta tcttttacac aattatatgg actttatcct ataatctgat aaattattaa
       61 gggcaaactt atgtgaaagg gtgataacta tgagcgataa aatacttatt gtggatgatg
      121 aacatgaaat tgccgatttg gttgaattat acttaaaaaa cgagaattat acggttttca
      181 aatactatac cgccaaagaa gcattggaat gtatagacaa gtctgagatt gaccttgcca
      241 tattggacat catgcttccc ggcacaagcg gccttactat ctgtcaaaaa ataagggaca
      301 agcacaccta tccgattatc atgctgaccg ggaaagatac agaggtagat aaaattacag
      361 ggttaacaat cggcgcggat gattatataa cgaagccctt tcgcccactg gagttaattg
      421 ctcgggtaaa ggcccagttg cgccgataca aaaaattcag tggagtaaag gagcagaacg
      481 aaaatgttat cgtccactcc ggccttgtca ttaatgttaa cacccatgag tgttatctga
      541 acgagaagca gttatccctt actcccaccg agttttcaat actgcgaatc ctctgtgaaa
      601 acaaggggaa tgtggttagc tccgagctgc tatttcatga gatatggggc gacgaatatt
      661 tcagcaagag caacaacacc atcaccgtgc atatccggca tttgcgcgaa aaaatgaacg
      721 acaccattga taatccgaaa tatataaaaa cggtatgggg ggttggttat aaaattgaaa
      781 aataaaaaaa acgactattc caaactagaa cgaaaacttt acatgtatat cgttgcaatt
      841 gttgtggtag caattgtatt cgtgttgtat attcgttcaa tgatccgagg gaaacttggg
      901 gattggatct taagtatttt ggaaaacaaa tatgacttaa atcacctgga cgcgatgaaa
      961 ttatatcaat attccatacg gaacaatata gatatcttta tttatgtggc gattgtcatt
     1021 agtattctta ttctatgtcg cgtcatgctt tcaaaattcg caaaatactt tgacgagata
     1081 aataccggca ttgatgtact tattcagaac gaagataaac aaattgagct ttctgcggaa
     1141 atggatgtta tggaacaaaa gctcaacaca ttaaaacgga ctctggaaaa gcgagagcag
     1201 gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta tgtacttggc gcacgatatt
     1261 aaaacgcccc ttacatccat tatcggttat ttgagcctgc ttgacgaggc tccagacatg
     1321 ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg acaaagcgta tcgactcgaa
     1381 cagctaatcg acgagttttt tgagattaca cggtataacc tacaaacgat aacgctaaca
     1441 aaaacgcaca tagacctata ctatatgctg gtgcagatga ccgatgaatt ttatcctcag
     1501 ctttccgcac atggaaaaca ggcggttatt cacgcccccg aggatctgac cgtgtccggc
     1561 gaccctgata aactcgcgag agtctttaac aacattttga aaaacgccgc tgcatacagt
     1621 gaggataaca gcatcattga cattaccgcg ggcctctccg gggatgtggt gtcaatcgaa
     1681 ttcaagaaca ctggaagcat cccaaaagat aagctagctg ccatatttga aaagttctat
     1741 aggctggaca atgctcgttc ttccgatacg ggtggcgcgg gacttggatt ggcgattgca
     1801 aaagaaatta ttgttcagca tggagggcag atttacgcgg aaagcaatga taactatacg
     1861 acgtttaggg tagagcttcc agcgatgcca gacttggttg ataaaaggag gtcctaagag
     1921 atgtatataa ttttttagga aaatctcaag gttatcttta ctttttctta ggaaattaac
     1981 aatttaatat taagaaacgg ctcgttctta cacggtagac ttaataccgt aagaacgagc
     2041 cgttttcgtt cttcagagaa agatttgaca agattaccat tggcatcccc gttttatttg
     2101 gtgcctttca cagaaagggt tggtcttaat tatgaataac atcggcatta ctgtttatgg
     2161 atgtgagcag gatgaggcag atgcattcca tgctctttcg cctcgctttg gcgttatggc
     2221 aacgataatt aacgccaacg tgtcggaatc caacgccaaa tccgcgcctt tcaatcaatg
     2281 tatcagtgtg ggacataaat cagagatttc cgcctctatt cttcttgcgc tgaagagagc
     2341 cggtgtgaaa tatatttcta cccgaagcat cggctgcaat catatagata caactgctgc
     2401 taagagaatg ggcatcactg tcgacaatgt ggcgtactcg ccggatagcg ttgccgatta
     2461 tactatgatg ctaattctta tggcagtacg caacgtaaaa tcgattgtgc gctctgtgga
     2521 aaaacatgat ttcaggttgg acagcg
//
LOCUS       DAZKOW010000292         2311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.190, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000292 DAZKOW010000000
VERSION     DAZKOW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.190"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            260..1171
                     /locus_tag="ACG912_003060"
     CDS             260..1171
                     /locus_tag="ACG912_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFQ0514266.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG912_003061"
     CDS             1324..1809
                     /gene="vanZ-A"
                     /locus_tag="ACG912_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFQ0514267.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          817 a          335 c          431 g          728 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt caaggaggta aggatggcgg aatgaaacca acgaaattaa
     1201 tgaacagcat tattgtacta gcacttttgg ggtaacgtta gctttttaat ttaaaaccca
     1261 cgttaactag gacattgcta tactaatgat acaacttaaa caaaagaatt agaggaaatt
     1321 atattgggaa aaatattatc tagaggattg ctagctttat atttagtgac actaatctgg
     1381 ttagtgttat tcaaattaca atacaatatt ttatcagtat ttaattatca tcaaagaagt
     1441 cttaacttga ctccatttac tgctactggg aatttcagag agatgataga taatgttata
     1501 atctttattc catttggctt gcttttgaat gtcaatttta aagaaatcgg atttttacct
     1561 aagtttgctt ttgtactggt tttaagtctt acttttgaaa taattcaatt tatcttcgct
     1621 attggagcga cagacataac agatgtaatt acaaatactg ttggaggctt tcttggactg
     1681 aaattatatg gtttaagcaa taagcatatg aatcaaaaaa aattagacag agttattatt
     1741 tttgtaggta tacttttgct cgtattattg ctcgtttacc gtacccattt aagaataaat
     1801 tacgtgtaag atgtctaaat caagcaatct gatctttcat acacataaag atattgaatg
     1861 aattggatta gatggaaaac gggatgtggg gaaactcgcc cgtaggtgtg aagtgagggg
     1921 aaaaccggtg ataaagtaaa aagcttacct aacactatag taacaaagaa agcccaatta
     1981 tcaattttag tgctgaggaa ttggtctctt taataaattt ccttaacgtt gtaaatccgc
     2041 attttcctga cggtaccccc actagcagcc atgctttttt tgaatctttg taggacgttt
     2101 taaagagaat tacccacttt aaaattaaac atgcgcttag aatcgcttta ggaaacacga
     2161 tccagtccaa taatcgtcga taaaaacttt tgaaaaaggt tggtgaaatt acctactttt
     2221 ggaataatca caaatcacaa gtgattaatc acttgtttat taagatatta aaagctataa
     2281 tttaaataaa gcgtgaattt tattacacaa a
//
LOCUS       DAZKOW010000293         2255 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.193, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000293 DAZKOW010000000
VERSION     DAZKOW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.193"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..553
                     /locus_tag="ACG912_003062"
     CDS             <1..553
                     /locus_tag="ACG912_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFQ0514268.1"
                     /translation="RGKVLSDMTVGVVGTGQIGKAVIERLRGFGCKVLAYSRSRSIEV
                     NYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQRMKQGAFLINTGRGPLVDTYELV
                     KALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQFLLKLQRMPNVIITPHTAYYTEQ
                     ALRDTVEKTIKNCLDFERRQEHE"
     gene            546..1577
                     /gene="vanA"
                     /locus_tag="ACG912_003063"
     CDS             546..1577
                     /gene="vanA"
                     /locus_tag="ACG912_003063"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0514269.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            1583..2191
                     /locus_tag="ACG912_003064"
     CDS             1583..2191
                     /locus_tag="ACG912_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFQ0514270.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          678 a          427 c          563 g          587 t
ORIGIN      
        1 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
       61 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      121 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      181 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      241 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      301 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
      361 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
      421 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
      481 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
      541 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
      601 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
      661 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
      721 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
      781 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
      841 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
      901 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
      961 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1021 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1081 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1141 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1201 tattgggaaa cagtgccgcg ttagttgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1261 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1321 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     1381 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     1441 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     1501 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     1561 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     1621 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     1681 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     1741 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     1801 gcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     1861 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     1921 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     1981 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2041 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2101 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2161 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaacggtt
     2221 ctgttgcaaa gttttaaata aagaataaaa tccct
//
LOCUS       DAZKOW010000294         2246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.194, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000294 DAZKOW010000000
VERSION     DAZKOW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.194"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG912_003065"
     CDS             <1..355
                     /locus_tag="ACG912_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0514271.1"
                     /translation="PDKLARVFNNILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTG
                     SIPKDKLAAIFEKFYRLDNARSSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFR
                     VELPAMPDLVDKRRS"
     gene            570..1538
                     /locus_tag="ACG912_003066"
     CDS             570..1538
                     /locus_tag="ACG912_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFQ0514272.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1531..>2246
                     /locus_tag="ACG912_003067"
     CDS             1531..>2246
                     /locus_tag="ACG912_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0514273.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYG"
BASE COUNT          665 a          423 c          554 g          604 t
ORIGIN      
        1 ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg catacagtga
       61 ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt caatcgaatt
      121 caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa agttctatag
      181 gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg cgattgcaaa
      241 agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata actatacgac
      301 gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt cctaagagat
      361 gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg aaattaacaa
      421 tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa gaacgagccg
      481 ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt tttatttggt
      541 gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact gtttatggat
      601 gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc gttatggcaa
      661 cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc aatcaatgta
      721 tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg aagagagccg
      781 gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca actgctgcta
      841 agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt gccgattata
      901 ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc tctgtggaaa
      961 aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg acagttggtg
     1021 tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga tttggatgta
     1081 aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg tttgatgagt
     1141 tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat acgcactata
     1201 ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc aatactgggc
     1261 gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg aaactgggcg
     1321 gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat tgcacccaaa
     1381 aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg ataatcacac
     1441 cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa accattaaaa
     1501 actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc aatactgttt
     1561 gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat agccgctaac
     1621 attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg tgtatggaaa
     1681 atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc tgtactctcg
     1741 ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga aatcaaccat
     1801 gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc catacaaggt
     1861 ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc agcaatttgt
     1921 atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac tcccgccttt
     1981 tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc tgtttttgtt
     2041 aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc ggacgaattg
     2101 gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga gcaggctgtt
     2161 tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt tgttggcgag
     2221 gtggaccaaa tcaggctgca gtacgg
//
LOCUS       DAZKOW010000295         2246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.195, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000295 DAZKOW010000000
VERSION     DAZKOW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.195"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACG912_003068"
     CDS             <1..355
                     /locus_tag="ACG912_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFQ0514274.1"
                     /translation="PDKLARVFNNILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTG
                     SIPKDKLAAIFEKFYRLDNARSSDTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFR
                     VELPAMPDLVDKRRS"
     gene            570..1538
                     /gene="vanH"
                     /locus_tag="ACG912_003069"
     CDS             570..1538
                     /gene="vanH"
                     /locus_tag="ACG912_003069"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanH-A/VanH-Pt family D-lactate dehydrogenase"
                     /protein_id="HFQ0514275.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSARGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1531..>2246
                     /locus_tag="ACG912_003070"
     CDS             1531..>2246
                     /locus_tag="ACG912_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFQ0514276.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYG"
BASE COUNT          664 a          424 c          554 g          604 t
ORIGIN      
        1 ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg catacagtga
       61 ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt caatcgaatt
      121 caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa agttctatag
      181 gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg cgattgcaaa
      241 agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata actatacgac
      301 gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt cctaagagat
      361 gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg aaattaacaa
      421 tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa gaacgagccg
      481 ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt tttatttggt
      541 gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact gtttatggat
      601 gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc gttatggcaa
      661 cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc aatcaatgta
      721 tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg aagagagccg
      781 gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca actgctgcta
      841 agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt gccgattata
      901 ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc tctgtggaaa
      961 aacatgattt caggttggac agcgcccgtg gcaaggtact cagcgacatg acagttggtg
     1021 tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga tttggatgta
     1081 aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg tttgatgagt
     1141 tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat acgcactata
     1201 ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc aatactgggc
     1261 gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg aaactgggcg
     1321 gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat tgcacccaaa
     1381 aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg ataatcacac
     1441 cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa accattaaaa
     1501 actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc aatactgttt
     1561 gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat agccgctaac
     1621 attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg tgtatggaaa
     1681 atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc tgtactctcg
     1741 ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga aatcaaccat
     1801 gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc catacaaggt
     1861 ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc agcaatttgt
     1921 atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac tcccgccttt
     1981 tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc tgtttttgtt
     2041 aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc ggacgaattg
     2101 gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga gcaggctgtt
     2161 tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt tgttggcgag
     2221 gtggaccaaa tcaggctgca gtacgg
//
LOCUS       DAZKOW010000296          966 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.233, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000296 DAZKOW010000000
VERSION     DAZKOW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.233"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            250..798
                     /gene="aac(6')"
                     /locus_tag="ACG912_003071"
     CDS             250..798
                     /gene="aac(6')"
                     /locus_tag="ACG912_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFQ0514277.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          340 a          142 c          204 g          280 t
ORIGIN      
        1 taattggttt tttttaaagc aaaagataat ctatatcatg atgaacaaac agagtttttt
       61 cctccaattg atggctttcc tgatctttcc tattttgaat aaagtgtttc tgtataattt
      121 ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg tagctaaaga
      181 tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca agaacaaggg
      241 ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa agatcagctt
      301 tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc agaagaagta
      361 gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga tgagttagta
      421 ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca tccattagtt
      481 gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta cttagaaaaa
      541 gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt agaccatgga
      601 acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc ttccatccag
      661 aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat cgtaggtgtc
      721 ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac gattattcct
      781 cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt caagaaataa
      841 gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa ttccaactta tttccgaaga
      901 agctactttt ggaacaccgt gtaccagagg ttgtgaaaaa gtcgcttagt ttcgagtagt
      961 aagaaa
//
LOCUS       DAZKOW010000297          949 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3397
            SAMEA115706687-rid22584263.guided.235, whole genome shotgun
            sequence.
ACCESSION   DAZKOW010000297 DAZKOW010000000
VERSION     DAZKOW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115706687
            Sequence Read Archive: ERR13196422
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 04-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002287734.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002287734.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 12:58:44
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,072
            CDSs (total)                      :: 3,014
            Genes (coding)                    :: 2,920
            CDSs (with protein)               :: 2,920
            Genes (RNA)                       :: 58
            rRNAs                             :: 3, 1 (16S, 23S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 39 of 94
            Pseudo Genes (incomplete)         :: 51 of 94
            Pseudo Genes (internal stop)      :: 22 of 94
            Pseudo Genes (multiple problems)  :: 15 of 94
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115706687-rid22584263.guided.235"
                     /isolate="VRE3397"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            2..739
                     /gene="erm(B)"
                     /locus_tag="ACG912_003072"
     CDS             2..739
                     /gene="erm(B)"
                     /locus_tag="ACG912_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFQ0514278.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          346 a          169 c          160 g          274 t
ORIGIN      
        1 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
       61 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      121 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      181 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      241 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      301 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      361 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
      421 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
      481 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
      541 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
      601 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
      661 aaacaattta agtaccatta cttatgagca agtattgtct atttttaata gttatctatt
      721 atttaacggg aggaaataat tctatgagtc gcttttttaa atttggaaag ttacacgtta
      781 ctaaagggaa tggagataaa ttattagata tactactgac agcttccaag aagctaaaga
      841 ggtccctagc gcctacgggg aatttgtatc gataaggggt acaaattccc actaagcgct
      901 cgggacccct tgtaggaaaa tgtcctaagt gtggcaacaa tattgtatt
//
